LOCUS       DAOSHM010000001       121158 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_107_length_121158_cov_23.031031, whole genome shotgun
            sequence.
ACCESSION   DAOSHM010000001 DAOSHM010000000
VERSION     DAOSHM010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 121158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 121158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..121158
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107_length_121158_cov_23.031031"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1150
                     /locus_tag="PK839_00005"
     CDS             <1..1150
                     /locus_tag="PK839_00005"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOG71149.1"
                     /translation="GGAPEGGIYSGTGVTGGTLDPAAAGAGTHTITYSYTDANGCSAS
                     ASEDMVVNGLPTANINTTDPTDWCEGTPINVNLTADVAESYQWLLDGQPITGETGQTL
                     IATQAGTYSVAATTNGCNSTGNTITITVISTPSVAITTEDPTTWCAGDEVSVQLTAIP
                     SSGDSYRWIKDEVTIAGETQSNYTANEPGNYRVEALFAGGCSSVSEIFSLTENPVPTV
                     SIATDTIQIDTSSTYTFDAGAGFVSYLWSDTSTGQTLLVDGAVTGTGEFTYWVTVTNE
                     FSCSASDTAVVVVSPVGGVPMQISWEISIYPNPSSGEFKLKVSGIEPGTYTLTILNAG
                     GQIVYGSTFSSSDTEITRAINIQTLSKGLYYVRFENTNKGFTRKIILK"
     gene            complement(1206..2324)
                     /locus_tag="PK839_00010"
     CDS             complement(1206..2324)
                     /locus_tag="PK839_00010"
                     /inference="COORDINATES: protein motif:HMM:NF020874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1972 domain-containing protein"
                     /protein_id="HOG71150.1"
                     /translation="MKIAILGTRGIPNSYGGFERFAEVLSQILVARGHLVYVLSPTFN
                     GKGRSVTRLSSMLFLVNVGVGKHLPHNFQTLHYDLKSLCWANTEEIDVILECGHSFAP
                     WLPLFKKTFRQKIVVNLDGLEHKRSKWSKPAKLFLRFSEYMAVHFVARLVCDNQALVS
                     YYAASYGVKPKVIPYGAFPLGEIPPRDVLAMYGIFEPYYLMVSRLTPENNIEMIARSF
                     VALGENLIIIGRYDTRFGESLYRKFEHFKNIRFLGPNFNQDDLNALRYHSLAYIHGHT
                     VGGTNPSLLEAMACKCFVIAHDNPFNREVLGDEGWYFGSTTELNDRIREFGRLPAMSV
                     LKAKQSNYTRIKNLYSWEAVADSYEELFSSINSQQDQG"
     gene            complement(2559..3785)
                     /locus_tag="PK839_00015"
     CDS             complement(2559..3785)
                     /locus_tag="PK839_00015"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG71151.1"
                     /translation="MLFKVFNRGKDESLYAGYESLLRSIPLFMGVDSEGLGKIAKSLV
                     SKSIKAGEVLFNKGDYDFALYIVVSGQVKAHDGNHTFATFGPRQFFGEYSMLDSLARS
                     TTITALRDTELLRLDQGQFNLLLDKNPLLSRALLKTLVWRLRDYNVIEAELTAKNQEI
                     KRQKEEMDVQRKELVSLNQTKDKFFAIIAHDLKNPFGAVLSLSELLAREFESFEPEKL
                     HMFIQQIYKYSNNTFNLLENLLQWSLLQTGRTVVNLKSLNMAEIINENIDLLKANAQQ
                     KGIELAWVNPDDHFVYCDRDMITTVLRNLISNAIKFTPAKGRIEVEVVKDSEYLKVMV
                     RDSGVGISPENQQKLFRIDSNPSTPGTNEEEGTGLGLILCKEFVEKNRGTIAVESTEG
                     KGSTFFFTVPRFENKS"
     gene            4341..6629
                     /locus_tag="PK839_00020"
     CDS             4341..6629
                     /locus_tag="PK839_00020"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HOG71152.1"
                     /translation="MKLKLNIRQKILVYILGPSIVVFAGIFTYTTRTSRDIAYNQAIK
                     LTDSYAHQYALNIEGWLNQDLAITRTLAAAFLEYKQMPFEQWQRLIYPMYDRIILTTP
                     HIDAYWDSWEMSNLDPTWDKPYGRYFYIVYKENGVYKTKAELRSLTGDPDTYGAMKKA
                     AKEIINEPYVSALQRGQLMTTISSPLVENGKFIGLIGIDLLLTRFQNLVNGIKPYPNS
                     YAFLLSNKGNFIAHPDSTIYQKNIAERLPELNSRFSILSNVQKGKAFSFTQTGENGQR
                     LYYTFSPINIGNTQTPWSVGIAVPINDILYEANKNYNISIAILILGLILLIAIIFFVS
                     SSITRPIKSITHLLQDLANGKVDSTLAIKHDVHDEIGKMALALSESIKGINDKTEFAR
                     EIGSGNLKAEVSLLSEDDHLGKSLLEMRDNLQKAKEDEEKRKAEDEKKRWTNEGLAKF
                     GDILRQNNNNLSKLSDEIIKNLVWYLNASLGGIFVLNENRETGDKTFDLVAAFAYDRK
                     RILNKSYQFAEGLVGACAAEKDIIMLTEIPQEYIEITSGLGGTNPNFLLLTPLVNEDE
                     VMGVIEIASLRRFEEHEILFVKEIAKSIATTLHSVKVNSLTAELLMKSKEQAEIMAAQ
                     EEEMRQNMEELQATQEEAARKTFELEGLANALSASSLIMEYDPAGVITSINEKYSELL
                     SISREDIINKHYSDIATEAIDKEKLDKLWQEIRSGKTKYRKVVLNIKGKEHTLVETYT
                     PILDTQGRLYKVLKIATDITNL"
     gene            7028..7588
                     /locus_tag="PK839_00025"
     CDS             7028..7588
                     /locus_tag="PK839_00025"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HOG71153.1"
                     /translation="MKKTLTVSLNGRSYELEEDAFKMLDEYLKQLESYFAEDAGKQEI
                     LSDIESRIADHFDESNRVRGVVIGADEVRRVIEIMGTPSDIADGEKVPKAEKPHRSYR
                     KLYRNMDDKVLGGVCSGMGCYWNVEPLLFRIIFIISIFWGGLGLLVYAILWIAVPPAL
                     TTTQKLEMRGEEVTAENIGKSFENKK"
     gene            7599..7922
                     /locus_tag="PK839_00030"
     CDS             7599..7922
                     /locus_tag="PK839_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019149489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HOG71154.1"
                     /translation="MEVENAKAQMRKGILELCILSVLSKGDAYANDLITKLKEAKMIV
                     VEGTLYPLLTRQKNLGLLSYRWEESPQGPPRKYYSLTDKGRTYLNELHRSWDELVETV
                     NTLRN"
     gene            8157..9722
                     /locus_tag="PK839_00035"
     CDS             8157..9722
                     /locus_tag="PK839_00035"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HOG71155.1"
                     /translation="MKKTVRVSLSGFSYNLEEDAYRLLDHYLEEIRVRLGSNADAEET
                     LRDIEERISELLTARMGQRETVPADMVQDIINQIGMPEEVTGNAEYSKDQWETQPPKK
                     RLYRDTENPVIAGVCSGVAAFFGVDPIVIRIIFIALLFARGFGLLLYIILWIVVPRAL
                     TPKQKLEMRGQPVTLSNMGKNISEEMSSVKSSVSQKVPRNFFERLGKFLGQVGFWLLK
                     FIMVLVKVVAIAIGIVLIVSMLFAFIVLVGAFFFSGITLPWLFPGTVVSSLNEFITSM
                     IDISSGLWITIPTFLILAIPIVALIYIGIRVLFRFKARDGKIAIIATGIWVASVLTLA
                     ISIFIQLRSLSFSGADRQVVQLSSQLPRNQQTIYLKAFPQYDEATLPSVYKFFDYSIT
                     TVQGEKVISGQPKLVIEKSDSDSISLILSKNARGFSSTNAAKNAADIIYPYSVKDSTI
                     FVDSRFTLPASVTWKGQTLTLSLLLPEGYSIYLDSSICGILDTDQPYSSHWPDEMVGQ
                     TWTMTRNGLRVKR"
     gene            9912..9982
                     /locus_tag="PK839_00040"
     tRNA            9912..9982
                     /locus_tag="PK839_00040"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9944..9946,aa:Gln,seq:ttg)
     gene            complement(10045..10893)
                     /gene="nfo"
                     /locus_tag="PK839_00045"
     CDS             complement(10045..10893)
                     /gene="nfo"
                     /locus_tag="PK839_00045"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HOG71156.1"
                     /translation="MKFVGAHVSAAGGVENAPVNANAIGATAFALFTKNQRQWVAPPL
                     SPKSIDLFRRRCEEFSYSPAQVLPHDSYLINLGNPDRDALEKSRNAFTDEMARCELLG
                     LDRLNFHPGSHLNKTTPEECLNLIAESINMALDKTHGVTAVIENTSGQGSNLGFTFEQ
                     LRYIIDRVDDKTRIGVCIDTCHAFTAGYDLLTPEAFTKTFSEFDRIIGFGYLRGVHLN
                     DSKKGLGSRVDRHENIGLGLLGAEPFRMLMGDPRFDGIPLILETPDESKWPEEIALLK
                     GFDREI"
     gene            11143..11589
                     /locus_tag="PK839_00050"
     CDS             11143..11589
                     /locus_tag="PK839_00050"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HOG71157.1"
                     /translation="MPAIRLTKEFRFEMAHVLEGYDGPCRNIHGHSYKLKVTVIGNPI
                     SQPGHPKLGMVIDFGDLKQVVETLITSRFDHSIAVLENSNIHRKLAGSDLEKVEALPF
                     QPTCENMVEYFARLLAPSLPEGVSLHHLLLHETATSYAEWYADDNP"
     gene            11968..12273
                     /locus_tag="PK839_00055"
     CDS             11968..12273
                     /locus_tag="PK839_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007655564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOG71158.1"
                     /translation="MKSIILDNIESIKALCLRYNVKSLYAFGSVCTEKFNDNSDIDLL
                     ISFYPMDYGDYADSYFELAEKLEDVFQRPVDLVTDKSLGNPYFIESINQTKTPIYEA"
     gene            12358..12594
                     /locus_tag="PK839_00060"
     CDS             12358..12594
                     /locus_tag="PK839_00060"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HOG71159.1"
                     /translation="MENKMLRRAVEREFEIIGEAMNQINKLDSTINISGKYQIINMRN
                     RVIHGYDKIDNEIIWGTIVRHLPKLKQEVEALLK"
     gene            13527..16298
                     /gene="polA"
                     /locus_tag="PK839_00065"
     CDS             13527..16298
                     /gene="polA"
                     /locus_tag="PK839_00065"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HOG71160.1"
                     /translation="MKKKLLLIDAYALIYRSYYAFIKRPIRTSKGLNTSAIYGFTRAV
                     LDAIRRFKPTHVAVAFDVSGPTFRDEIYPDYKAQRQQTPEDIKISVPIIKQLLDALQI
                     AVVELPGYEADDVVGTLATQANPDEFDVIMLTPDKDYGQLLRKNVSIAKPGRSGNDMQ
                     LVTADEFCRMYDIQNPKQFVDILALWGDVSDNIKGVEKVGEKIAAKLISDFGSIDNIL
                     NNTSKLNDSLRKKIEDSREKLALNKKLVTIVTDLDLKVDIDKDLVSREPNTNELLKLF
                     QELEFRSLIREFDRQPVAAKKEYIQGSLFGEDPEQKVTVIQNLTTIKDIKADYTAVTT
                     AEERKLVVEEISGCTEFAFDTETTGLDPISDSLVGLSVATKPLKAWWIPVPDDRESVS
                     KILAEFKPLFENPQVAKIGQNIKFDMQMLANYGISVKGELYDTMLAHYLLDPDSRHNL
                     NHLSEIYLGYKPIEIEELIGKKGSKQANMRNVPLEKITPYACEDADIALQLKLRLFAE
                     LESQGLTKLYFLLEAPLVEVLAQMERNGVCLDVQSLQEYGKILNSELLKLDAEIKAMA
                     GVPDLNISSPKQLGEVLFDKLRIATDVKMTKTKQYSTSEEELLKYQSAHPIVGKILEF
                     RSIKKLLSTYIETLPGLVNTKTGRIHTSFNQAVAATGRLSSNNPNLQNIPIREENGRE
                     IRRAFIPSPGSYSMLSADYSQIELRLMAHMSGDSAMMEAFRNGEDIHAATAAKIFKVP
                     LEQVSREQRSRAKTANFGMIYGISAFGLSQRLGISRTEAKELIDEYFRTYSGVKRYMD
                     SCIAQARERGWVETLFGRKRYLNDIKSSNQTVRGMAERNAINAPIQGSAADIIKLAMI
                     GIHSEFKRLKLQSKMIIQVHDELVFDVYQPELEQVKEIARSQMEGAAALQIPLVVDIG
                     VGKNWLEAH"
     gene            complement(16327..17355)
                     /gene="dusB"
                     /locus_tag="PK839_00070"
     CDS             complement(16327..17355)
                     /gene="dusB"
                     /locus_tag="PK839_00070"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018697161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HOG71161.1"
                     /translation="MRIGEVELGEKPLFLAPMEDVTDPSFRYVCKQFGADVMYTEFIN
                     ADGLIRDAGSSVAKLNIYDYERPIGIQLYGHIIESMVEAARIATACRPDIIDINFGCP
                     VKKIANRGAGAGMLRNINAMVEMTKRIVEATSIPVTVKTRLGWDDESKNIVEIAERLQ
                     DVGIKAITIHGRTRAQLYKGKADWTLIGAVKANPRITIPVIGNGDIDGPVIAGEMFEK
                     HGVDGIMVGRASFGRPWIFREIKHYLSTGQLMEPLTASQKVEIAKLHFQKSLEVKGHK
                     TGILEMRRHFSAYFKGLPNFKEIRLKLLTSTEPEVIEGLLELIRSTYGDFVADGSINP
                     SPWADSAD"
     gene            17609..18526
                     /locus_tag="PK839_00075"
     CDS             17609..18526
                     /locus_tag="PK839_00075"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOG71162.1"
                     /translation="MRAGILSNLSDAKKIVALIILVLLGSFLFTLLGYLVIMAVYGTD
                     SLGWLMNVTISNDTIGALKFLQIVQTIGMFVVPGFTAAWLFSSHPFGYLGFKPSTVQN
                     FITVAVMMVISIPAINLLASINELIPLSGWMVEMERNAEILTEAFLKTDSVGTFLLNL
                     LMIAILPAVGEEIIFRGILQKHLVSATRSRWLGILITAALFSAFHLQFRGFIPRFALG
                     VIFGYLYEWSRSLWLPMLAHFTNNAIAVIAFLLMGSEISVNEIEEVGSLQDIWAIGIV
                     SFLAVALLLRFIWKTNTRQSPVSQNMVKS"
     gene            complement(18589..19323)
                     /locus_tag="PK839_00080"
     CDS             complement(18589..19323)
                     /locus_tag="PK839_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG71163.1"
                     /translation="MPYRRLPNTDSARIRALKAAYSKGKLNPPSQLAFSQSTFSKVEL
                     FINSFQNALAHYKVAYNQQVESGKEYSQILKKAKLYLSHFIQVLNMAIARGELPVSVR
                     EIYGMDLDENRMPSLTTEKELIEWGKKIIDGEMQRLQKGQTPITNPTIAVVRVRYEQF
                     LEAYNHHKILQQNTRRLLNDVNNLRVTADSIILSIWNEVEDYFKDLPDEERRLKASEY
                     GVVYVYRKNEIKDFRLEDRTLLKLFN"
     gene            19559..20755
                     /locus_tag="PK839_00085"
     CDS             19559..20755
                     /locus_tag="PK839_00085"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HOG71164.1"
                     /translation="MYGKIQKDLQKQLEAIVNDGLYKTERAISTPQGAEIKANGKKVL
                     NFCANNYLGLSSHPAVLEAGKKAIDEWGFGMSSVRFICGTLELHKQLEAKLTDFLGTE
                     DTILYSSAFDANGGVFEPILGEEDAIISDELNHASIIDGVRLCKAQRFRYKNNDMDDL
                     ERVLKEASGCRYKMIVTDGVFSMDGIIAQVDKICDLADKHDALVMVDDSHATGFVGKT
                     GRGTSEYCNVMGRVDVISTTFGKALGGASGGCISGRKEIVDMLRQRSRPYLFSNSLMP
                     AIAGSTLMVLNMLSKSTELRDKLWENTALFRKGMTDAGFRIVPGVHPITPILLGHLPN
                     DAKLSQDFADMLFDEGIYVTGFFYPVVPKGKARIRVQLSAAHSRENVLFAIDKFTKIG
                     KKLGVI"
     gene            complement(20944..22056)
                     /locus_tag="PK839_00090"
     CDS             complement(20944..22056)
                     /locus_tag="PK839_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOG71165.1"
                     /translation="MPKRKRHYSENPARRIKVGTAIIPVIIGLGVVFLMLFREFNPQA
                     FLQISFTWRSFFWILVAALLMAGRDMGYTFRLMTLSEGKFKFWKAFRVIMLWEFTSAI
                     TPSAVGGTSVAVVYVNKEGLTVGRSSAVVMITSFLDELYFVVMFPILVLLTGPHELFT
                     LASSPAGGMAKDLIIFSIVGYSLKLVWVLGLSYGLFINPRGLKWLIMMIFKLPLLRRW
                     KQGANKAGSDIITSSEEFKKKPFSFWAKAVASSFLSWSSRFLVINALLLAFFDVNHHL
                     LIFTRQLVMWIIMLVSPTPGGSGFAEFVFTRYLSDFIPVGAESLTSIAVGMAMLWRLI
                     SYYPYLFIGAIVFPRWVRKKFGREKGHRSREIATTK"
     gene            complement(22158..23738)
                     /gene="nadB"
                     /locus_tag="PK839_00095"
     CDS             complement(22158..23738)
                     /gene="nadB"
                     /locus_tag="PK839_00095"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008143367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HOG71166.1"
                     /translation="MSTFQCDFLVIGSGIGGLSYALKVADYGKVILVTKAGIDDTNTS
                     MAQGGIAAVTYPPDTFEKHVNDTLICGDGYCNEEVVRMVASEAPAQINQLIQWGVKFD
                     RKPNGQYDLAREGGHSEHRILHHKDITGAEIQRALSAGVRQHPNIQVFENHFAVELIT
                     QHHLGRVIHRGDPDITCFGAYVLDQKTQAVHTFLGRSVVVATGGSGNIYHTTTNPRTA
                     TGDGIAMVYRAKGAVENMAFVQFHPTSLYHPGERPSFLISEAMRGYGAVLKTSDGKEF
                     MQKYDPRGSLAPRDITARAIDSEMKQRGDDFVYLDVTFKDPDDTINHFPNIYQKCLSV
                     GIDVTKDMIPVVPAAHYLCGGIKVDMNGQTAIKRLYAIGECSSTGLHGANRLASNSLM
                     EAIVYAERAARHSLATFKSINNSTRIPDWDYEGTTHNEEMVLITQSYKEMQQIMTNYV
                     GIVRSNLRLSRALNRLDIIFEETEELYKKSILTRELCELRNLIQIGYLIIKDAQNMHE
                     SRGLHYSIDYPRQMGTEF"
     gene            complement(23830..24603)
                     /locus_tag="PK839_00100"
     CDS             complement(23830..24603)
                     /locus_tag="PK839_00100"
                     /inference="COORDINATES: protein motif:HMM:NF005596.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HOG71167.1"
                     /translation="MYPDYHTHTNLSDGRDTHEEMFASAQIRDVSEIGFSDHICVKPV
                     SWAMDVRNIPAMVEAVQMLKQKEGVVRVKQGAEVDFIPGRETETRELLSFIPLDYCIG
                     SVHFIDDWNFDTDILPYDQISINAFYKTYFKLVCQSAKSGLFDIIGHCDLAKKFAYYP
                     TISLDRIFEETARVFSDYGVTVELNTSGKNKPCAEFYPSLRLLEILHHYKVPVTLGSD
                     AHVEQNIGQYFTDAIALLRYVGYKEVSTFSERKRSAIRI"
     gene            24864..25661
                     /locus_tag="PK839_00105"
     CDS             24864..25661
                     /locus_tag="PK839_00105"
                     /inference="COORDINATES: protein motif:HMM:TIGR01533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase, lipoprotein e(P4) family"
                     /protein_id="HOG71168.1"
                     /translation="MRTNLLGIIAISGVISLMVSCQPQAEKQEYNQDHMLYSTIWYQH
                     SPEMQALYYQAFNIAGERVRQQSSHKTSKPKAVVVDIDETMLDNSPFQAQEILDNREY
                     STEFWKEWTSLARAKALPGAVEFSKLCDSLKVTLFYITNRSADEAASTLKNLDSLGFA
                     FAKPEYLLPKENESSKKQRRERIAEQYDIILLVGDNLNDFSEIFENRGDDWGSGLVGK
                     NKSDFGSRFIILPNPMYGDWEKNIYHTRGLSPQQKDSLRRSLLNGFR"
     gene            complement(25888..25962)
                     /locus_tag="PK839_00110"
     tRNA            complement(25888..25962)
                     /locus_tag="PK839_00110"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(25927..25929),aa:Arg,seq:cct)
     gene            26219..27496
                     /locus_tag="PK839_00115"
     CDS             26219..27496
                     /locus_tag="PK839_00115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HOG71169.1"
                     /translation="MSKELMHETLNTLGLEEDDEPPGSGQLSPNYGLITATSHQHKSP
                     SCNWPFPISKRSLEFLIKYFPNATVEDWMSWKWQLKNSFTTSEKLKCFLDLSREECEA
                     IDHPKHSLPLRITPYYASLLNGKNSQDPLRRTMIPTVNELTTSAGESCDPLNEGHSSP
                     VPNLVHRYPDRVLFLSTGFCSAYCRYCTRSHMVAKDKCHVGVNAWLPAINYIREHTEV
                     RDVLISGGDPLTMSDAHIEYLLSQLRSIPHVEMIRIGTKVPVVLPHRITPKLIKILRK
                     FHPLYISIHFTHPDELTPETAEACRRLADAGIPLGSQTVLLKGINDNVDTMKSLFQGL
                     LKIRVRPYYLYQCDPIPGSSHFRTSVEKGLEIIKGLRGWTTGYAIPHFVIDAPGGGGK
                     IPLLPEYYQGRNEEGYVVLKNYEGKTFYYPDLH"
     gene            27671..28654
                     /locus_tag="PK839_00120"
     CDS             27671..28654
                     /locus_tag="PK839_00120"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOG71170.1"
                     /translation="MTVGLTYDLKSDYLKNGYSHEEVAEFDSDETIDSIESTLQSLGY
                     FTERVGNIINLTHALANGKRWDMVFNIAEGLHGIGREAQVPAILDAFQIPYVFSDPLV
                     LALTLHKGQAKRVVRDCGVPTADFAVITSLSEVEKIALPFPLFVKPAAEGTGKGINGQ
                     SKINSKEVLYATTARLLEEYRQPILVETYLPGREFTVGIVGTGDKARCLGVMEIVISN
                     RAKDGTYSLHTKENWKGIVDYYMATGEIESLCAETALMAWNCLGCRDGGRVDLKMDAS
                     GKPNFIEVNPLAGLNPFHSDFPMLAQKNGIDFTMLIQLIMDSAMERFNHTA"
     gene            28651..29637
                     /locus_tag="PK839_00125"
     CDS             28651..29637
                     /locus_tag="PK839_00125"
                     /inference="COORDINATES: protein motif:HMM:NF014686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOG71171.1"
                     /translation="MNRQTIHAVILYNELSESAGPDERDVLDQVALVAEMLNKLGIES
                     QRAEFSLNLQKVRDELSRLKPDFVFNLVESVNNRGNLIFLAPSLLNSMGIPFTGGSLE
                     TIFLTSSKTLTKQLMQDAGIPTPFWYQGNGSFIPDRDKRYIVKPIWEDGSLGLDEDSI
                     FEGSNPKLAEILNSIDYNTHFIEEFVDGREFNISILAGQNGPQLLPPAEIIFRNYAPD
                     KPHVVGYSAKWIEDSFEYINTNRTFDLSPNDQPLLSTMGDICLHCWKTFRVNGYARVD
                     FRVDNAGNPWVLEINVNPCISRDSGFYAACMQAGIPFDEAVRSIIADIPFQI"
     gene            29654..30136
                     /locus_tag="PK839_00130"
     CDS             29654..30136
                     /locus_tag="PK839_00130"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOG71172.1"
                     /translation="MDNVTFRSTPTPADKAGIREILSSSGFFHDFEIDVAIELVEEAL
                     AKGTESGYYFVFADHMGTTISYACFGPIPCTRHSFDLYWIGTHQDYRGKGLGKQVLQR
                     VENEIKQLNGRKIYIETSSKPMYEPTRQFYLSNAYENEAILKNFYDEGDDKVVYSKSL
                     "
     gene            30521..31012
                     /locus_tag="PK839_00135"
     CDS             30521..31012
                     /locus_tag="PK839_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HOG71173.1"
                     /translation="MQEKFRVDVIDQKILSYLVKNARIPFLEIARECGISGAAIHQRV
                     KKMEDAGIIAGSRMIVKPKALGFDVCAFVGVQLVSTNLYSGVIEALKLMPEVVECHFI
                     TGEYALLLKIYCRDNEHLMDVLVNTIQNIPGISRTETFISLDQAIERPFYVKDKSVVH
                     NEK"
     gene            complement(31081..31299)
                     /locus_tag="PK839_00140"
     CDS             complement(31081..31299)
                     /locus_tag="PK839_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG71174.1"
                     /translation="MKSKKDFRKPVKNDSYDSDEVKMVKKLKPVKKEKNAKRSIFSEI
                     DELDDLVDFKGTVLDEKFEDYDEEEEEL"
     gene            31708..32220
                     /locus_tag="PK839_00145"
     CDS             31708..32220
                     /locus_tag="PK839_00145"
                     /inference="COORDINATES: protein motif:HMM:NF033644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UpxY family transcription antiterminator"
                     /protein_id="HOG71175.1"
                     /translation="MDKGEANKRWYALYTKPRNEKKIYSRLVEKGVEAFLPLQKRLKQ
                     WSDRKKLVEEPLFRSYIFVRIDAREYYEVLNTPGIVRYITFEGKAVPIPDRQIEQVKQ
                     LLVQDLEIEPVEEQIPPGALVEVRFGNLMGITGELVEHAGKKKVIIRIDHVSHSLLVT
                     LPKDYVARAV"
     gene            complement(32265..32993)
                     /gene="pepE"
                     /locus_tag="PK839_00150"
     CDS             complement(32265..32993)
                     /gene="pepE"
                     /locus_tag="PK839_00150"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HOG71176.1"
                     /translation="MDSQSKKTVKMLLISNSTNAGEAFLEHPKEELKRFLNGTKRVLF
                     IPWAAVTFSYDEFERKVQERFSEFGVEVDSIHHYRNVKTAVQEAEAIVVGGGNTFKLL
                     KTIQQHDIVETVRNKVLDGTPYIGWSAGANVACPTICTTNDMPITEPDNFGAFSLIRF
                     QINPHYLDANPAGHAGETREQRIMEFLEMDPYVYVVGLREGSMILLENVALTLKGPKS
                     ARIFKKGIPPFEVNPGDDMDFLFE"
     gene            complement(33030..34979)
                     /locus_tag="PK839_00155"
     CDS             complement(33030..34979)
                     /locus_tag="PK839_00155"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG71177.1"
                     /translation="MKGSLAAILLAGSLSLVTPRLWGQPLSLDSLLHELSTVKSSEGK
                     YKILSSIAISYSDSSYSKSLEYWLEALEVAKKLESSKYTADILHQIGFTYLKMGEPKS
                     ALANFQNAAELYVSLNDNADLAGVYNDMGLIYRSWGRYDSALEFYLKSYKLYERLANA
                     EGMAMASNSIGQIYYYRENYTKAVDYFSSYLEINQGLGNYRAVAGACNNIASAYMELN
                     HLDRALDYYMKAFKIYDSLGVKIGVGIIQDNIGSLLYSKGDYRNALLYHFNALQIFNE
                     LKSDTRKCLTLKNIGSVYLKQNRPDVAVAYLNQSLDMAVGTGQKDVARDVHQTLANAY
                     EVIGSFPKAYYHLKNYQVLKDSMLNAETIEKIETLQAQLEMENQAAQVSLVQHKLKLQ
                     RQVAWTVSGLLAMMGGLLVLLYRENKKKNAALAEEQKFKESTVKRLDAFLQNTLKVAD
                     DGTVGGFFSDSWKLQPANSTQGTCKLLSFKVNNSTLAFMQVSDSQRVSPNLITLCLCC
                     WVKSNAHRLMISWVNFMKEMRENFAADPLTSDLDPSQYCILPFTINGDFVLNLTEEGF
                     AVWHSNKLIFPKAFQCPELVKGDTLYLYFTTIGLPLPDREQREMEKIIGSVASMEFSL
                     QPQAMQNLLKTFELENFTYIFALRI"
     gene            complement(35293..37833)
                     /locus_tag="PK839_00160"
     CDS             complement(35293..37833)
                     /locus_tag="PK839_00160"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HOG71178.1"
                     /translation="MSTTIHPESLQGLSKAEAARRLIEEGYNELPSSKPKNVFQIALG
                     VVKEPMFILLVACGTLYLILGDVNEGLMLLSFVFVIMGIEFYQEKKTERALDALKNLA
                     SPRALVIRDGETVRIAGREVVTGDYVVLQEGDRVPADGVILSSINLSADESLLTGESV
                     PVRKREWLEGDGSFIPGGDDIPMVFSGSMVVNGNGVFRVTATGVNTEIGKIGKALESV
                     KEEPTRLKREMAVLVKRLAVIGVILCLLVVIIYTITRGDLLNGFLAGITLAMAMLPEE
                     FPVVLAVFMALGAWRISRNNVLTRQPAAIETLGSVTVLCTDKTGTLTQNRMTVTQLYN
                     GTNHIAVTSGDTFTEPFHQVIEYAILSSQVNPYDPMERAIISIGDKYLQNSEHIHKDW
                     VMEKEYPLSKELLAMSRVFSNTGTGRRVIAAKGAPEAIFDLCHLPAAEVGKHEKAVAE
                     MASRGLRVLGVARAELSTENLPTIQHDFNFEFMGLIGLSDPIRENVPEAVAECYRAGI
                     RVIMITGDYPVTATNIGREIGIKNPELYITGQDLETMTEEELGERIKQVNIFARVVPE
                     QKLKLVNALKRNDEIVAMTGDGVNDAPALKAAHIGIAMGEKGTDVAREASSLVLMDDN
                     FASIVKAVRMGRKISDNLQKALAYIFAIHVPIAGLSLIPVFFANLPLILWPVHIVFLE
                     LIIDPACSIIFEAEKEEKNVMTRPPRNINQPFFGVGKILMSCTQGLGILAVSLLVYFI
                     GFRMGYAEKAVRAFTFITLIVSNIAVILSNRSWSDSIFKIIMTPNKTVKWIVGGAVLF
                     LLLIVNIPALQNLFLFDKIGWLELGICTLAGLSTIIWFELYKHFKRII"
     gene            complement(38018..38431)
                     /locus_tag="PK839_00165"
     CDS             complement(38018..38431)
                     /locus_tag="PK839_00165"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOG71179.1"
                     /translation="MESMEKVIAGLSNMIGDAEEKAKEQFNTLNLTITQMHYLETINL
                     LGNPNITELSAQLKLSKPTVTVSVDKLIEKGCVYKIQSDEDRRSAHIHLTEKGKLVNQ
                     MHDYAHRKIAEMVKNRLSAEELTLLERLLSKVLVE"
     gene            38734..39237
                     /locus_tag="PK839_00170"
     CDS             38734..39237
                     /locus_tag="PK839_00170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG71180.1"
                     /translation="MNNAIATPQDNHERLLRMVEEIFATSGDPRQLNITPEVIDRLKQ
                     LHPSTVLEHVSENGTVAWVVLIPTTLELMNRFVAGEISEKELFELTPLGIKYEALYLC
                     SALVLEEYRRQGIVKQMVTAAAQSIRKEHPLKAIFVWPFTKEGELAAESIATTLNLPL
                     YKFQHDK"
     gene            complement(39238..40596)
                     /locus_tag="PK839_00175"
     CDS             complement(39238..40596)
                     /locus_tag="PK839_00175"
                     /inference="COORDINATES: protein motif:HMM:NF017396.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S28 family serine protease"
                     /protein_id="HOG71181.1"
                     /translation="MKRMPQQQRPLRIRGLILSIFFPVLMVVILSGNPKPITPTEVLS
                     SIALVDSFRAIPSDNPFSETYEVWFRVPLDHTNPHSQYFPLRVYYSHNGFNKPMVVVI
                     DGYTMYTSKANEITSIVDGNQITIEHRFFSQSRPLDSIPWSYLNIRQAAADAHLVISA
                     FKPFYHGKWISTGISKSGQATIFHRRFYPADVNVSIPYVAPLNFSSEDERVYKFLDSV
                     GTPECRKKIYDFQIQLFERKSEIMPLFKELSASKGWKFTMGFEKAYDLSVLEYSFAFW
                     QWGFNCSSIPASSSTAKELFEHWLEVNPFSFFEENEVDKVRPFFYQAMTEIGMYGYRV
                     KPFSRYLSDTANITFSFAMPRGHRAIFNASAMSDIDRWVKDSGSYMLYIYGQNDAWSA
                     TAVDPGRKTNAVKMVCPGGSHSSRIKDFPPELQDSIYKVLGRWLDLKLPANGYTNSSS
                     GL"
     gene            complement(40598..42031)
                     /locus_tag="PK839_00180"
     CDS             complement(40598..42031)
                     /locus_tag="PK839_00180"
                     /inference="COORDINATES: protein motif:HMM:NF012372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HOG71182.1"
                     /translation="MDASSNSYLSRINSWCKRGKSFDLAVITHYLQNYTQNISAGYSG
                     EAVRIFDNYSMHNFIEHGEPDNGSDLLAILLDEGVKVPFPSPKQPSFTFIDLFAGIGG
                     FRLAMQELNGKCVFSSEWNTESQATYMVNFGEFPFGDITLQEVKDLIPYGFDLLCAGF
                     PCQPFSKGGHRQGFDDTRGTLFFDICQIVMKHRPKFLLLENVANLAGHDGGNTYSVIT
                     RSLDELGYYFPRNPMILSPDKFGVPILRPRLYIPCVRKDLAAGKEDFIWNFEEEIWKH
                     PSENQLTVNDIVDPNVDDCISPYEEKVLTAWDEFYRGIDQKVIGFPVWMDFFRFEGDL
                     SQFPVWKATFIRKNIDLYRRNRSFIDKWLAKWDNLEWCSKTHRKFEWQAGDGYSSLFD
                     CLIQFRPSGIRVKRPDKFSTLVAMNHRQIIGRFRRRISVEETKLLQSFPKEYNLIGPP
                     ATALRQLGNSVNVRVVKNIFGLVIKNF"
     assembly_gap    42230..42329
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            42363..42451
                     /locus_tag="PK839_00185"
     tRNA            42363..42451
                     /locus_tag="PK839_00185"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42397..42399,aa:Ser,seq:gct)
     gene            42458..42534
                     /locus_tag="PK839_00190"
     tRNA            42458..42534
                     /locus_tag="PK839_00190"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42492..42494,aa:Pro,seq:tgg)
     gene            42793..43890
                     /gene="meaB"
                     /locus_tag="PK839_00195"
     CDS             42793..43890
                     /gene="meaB"
                     /locus_tag="PK839_00195"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008764290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HOG71183.1"
                     /translation="MKQRKNKDDESALFVAPGIDQPPVVNEQAVQKFRQQKRRQPSID
                     EFVGGILARNNTILSQAITLVESSLPRHYELAQQLIERCLPYSGNSIRVGITGIPGVG
                     KSTFIESLGTMLTSQGHRLAVLAIDPSSTRSKGSILGDKTRMESLSADANAFIRPSPS
                     AGSLGGVARKTRETIILCEAAGFDTVFIETVGVGQSEVSVHSMVDFFLLLMLAGAGDE
                     LQGIKRGIMEMADLIAITKADGANISRSKLAQAEFQNALHLFPMPESGWVPKVVTCSA
                     LNKVGIDEIWREMVAYADFTRKNGYFVSRRNRQAKYWMHETINEALKNKFYADRLIHE
                     QVRELEKEVVANRLSPFAAAQLLLDRYFSLK"
     gene            44029..44556
                     /locus_tag="PK839_00200"
     CDS             44029..44556
                     /locus_tag="PK839_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018451909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOG71184.1"
                     /translation="MDKKQFLSTILNRKSVRSFTGKPLGQDDMELLLRAGMAAPSAHN
                     LQPWKFIAIVDRQILNSLAEKLPYAKMLFEAPAAIVVCGDLTKTGSSPKDYWVQDCSA
                     ASENILLAAEALGLGAVWTGVYPRAERVETVQGILNLPSEIVPLNVIPVGTPKGEHQA
                     KDKFKSENIQWNRWP"
     gene            complement(44553..45929)
                     /locus_tag="PK839_00205"
     CDS             complement(44553..45929)
                     /locus_tag="PK839_00205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HOG71185.1"
                     /translation="MVKTAKPKKEKKESRIQRLKKKFEQIYSFITHDIWVLQVDELSP
                     KLKAFYKYLRVGLLALRGFNEKKVSVKASALTYYTLMSIVPIFAMVFGIAKGFGLDKF
                     LEQQIRSSLEGQEEVMNKLITFSSSLLERTGGGIIAGIGVVLLFWSVVMVLSSIENAF
                     NDIWQIEKPRTYVRKFTDYLSIMVIAPILLLAASGIQIYLSTQVSTIAEKSQIIHIIS
                     PVLLSGFKLVPYLLIWILFSFLFITLPNTKVSIVSGIIAGILSGTAFLIVQWAYVYLQ
                     IGVSKYNAIYGSFAALPLFLVWVHTSWLIVLFGAEVSFAYQNVEMYEFEKETDQISHF
                     NRKVLSILILNTIIKRFNNGEEPLTSLDLSKQLKIPQRLMRNLLTQMVECGFLNEVVT
                     PIAKNYAYQPARHIDHLTLAYIEDKLDNHGLKIEPNSDDYKHIAEVYRNFNEKYKALS
                     KETLIGSL"
     gene            complement(46044..47168)
                     /gene="dprA"
                     /locus_tag="PK839_00210"
     CDS             complement(46044..47168)
                     /gene="dprA"
                     /locus_tag="PK839_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOG71186.1"
                     /translation="MSADPELVYKIGIELIPKVGSISAKKLIAYCGSAEAVFKQKKGS
                     LLKIPGIGEATAAEIVAQTVLSRAENELLFIEKFGIKVLYFTDAAYPYRLKQCEDGPV
                     VLYVKSNINIDFNTDKVLSIVGTRRATEYGKAMCEDLVSGLAEKGYRPIIISGLAYGI
                     DVTAHRAALKRNLPTVAVLGHGLNTIYPQSHRSVAKAILNQGALVSDFTSDAVFDRNN
                     FLRRNRIIAGMADATLVVESALEGGALVTADIANSYNRDVFAVPGNVNSVYSQGCNQL
                     IKQNKAALVESADDLEFLMGWTTSKVNSSRQYSINFDKFSADEQKILNFLKEKGEETV
                     DVIAMDTGLQVSKVLSLLLNLEFAGVVRSMPGKVFALAIG"
     gene            complement(47165..47647)
                     /locus_tag="PK839_00215"
     CDS             complement(47165..47647)
                     /locus_tag="PK839_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG71187.1"
                     /translation="MKFDNDDTVTGIHSAKLIMAIIAIILIVLLIATGLSDFLLRTTG
                     IDRDWAIAIILLLYILFSGYFYLRRASYFSYNDEGKKIIIRYFLLRPFKAPKISIEVD
                     KSEFYRFTIEKKFLRRELSIFVRNGNKVSRYPAISISSLTGKQLEQLVAALNSYVKGK
                     "
     gene            complement(47819..48967)
                     /locus_tag="PK839_00220"
     CDS             complement(47819..48967)
                     /locus_tag="PK839_00220"
                     /inference="COORDINATES: protein motif:HMM:NF013761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HOG71188.1"
                     /translation="MIALDNTGNAFQYLSNKELKRACRLFTLLSKLGGMNRSGKANSW
                     ASIPPVNFLLKNRIFDFFSGGLTLETCERVGDELWGYGVYSSPAPLVECNDKQLVFDE
                     YLRAIRIARDFPNHAFTLVKLSTLVEPRILRLVSQNAMLNHADKGIYTEFANRVRLLC
                     NEAFSSGKSIIIDAEHVDYQPAIDSIASEMMMLHNGDRATVYQTFRFDMVETFERLVE
                     QLRIASKNGYILGAKLLRGTLTHREVGESEKILFQNTQNDINHSLNEAIKFLLNNLAS
                     ASFIFGTHNHDTILYILSLMENLGVSPGDPQVFFAHHLGLADHLSFNLALSGYNVVKT
                     IPYGPKNIVEKGIVSSIIQHYSASHLREERHFLRKEVKRREKLVEGET"
     gene            complement(49164..51071)
                     /locus_tag="PK839_00225"
     CDS             complement(49164..51071)
                     /locus_tag="PK839_00225"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HOG71189.1"
                     /translation="MKRILNLTMLLGLLWACNPMEDTYNNLDEARKPYKENITYTLDA
                     TDYSNASKYALADAQNHADSASAQLIKDQQAFNQRFTAKNYVGKVLANRFVALNLGST
                     ANVTYNVNLENTGYLTAYSSPTLYTLTSDNYQSVGGAVANTGYFIPSMPAEEFIPQIL
                     TAAISSPTANQQCLVSYKSSDFEPSSTTTRTDTLFKENFNENNLDKFDTVSVKGAQKW
                     AASAYSGNYFAKISGYSGGVNNENEDWLIIPAIDLSGVQSATLNFTTAWKYDGNDLLL
                     KYSTNYSGSGDPNAASWTNLSFDLGTGNFVFCPSGNVPLPKAKIYIAFVYTSTTSASR
                     TWEVDDVVITAKVLAKGKSVTADLFKNYNDYYRFTGSKWEKSKGVLCVQPFEYSLMGL
                     TGNAFTNTNPASRYIPTYLSQKYPYAQDGDTCIMVYNYPNANTKVAGKYQNSAGSWMV
                     YNGIVQQSGQFIHTGEHWIFDPTVHLTPSASDLQLLVDYVYLTYGRQYGSSYGNDEFY
                     FGASAYYKNFDLRLSTRSQYNVPGFAELATDEEKIALTWQRLQEGLTILLKLKYANDA
                     VLEVDGIPVYYWVTFNTYENDLAKKTYVGIFKCTQPAPDPIFERDQDAEDLAVSDGKL
                     LQSAVSWNREK"
     gene            complement(51195..53753)
                     /locus_tag="PK839_00230"
     CDS             complement(51195..53753)
                     /locus_tag="PK839_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013760737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOG71190.1"
                     /translation="MMKVIKKILLTVCIVGTAGVAFSQGIVKGVVVDSNSGEALVGAA
                     VAVENTTIGTLTSLDGSFVLKVPVEKGTIKVTFVGYSDKTIEVTIPSGTEKDLGQIQL
                     TASSIGLEEVLVVSSFVRDRTTPVAVSTIEPKIIAEKLGNKEFPEILKSTPSIYATKS
                     GGGFGDSRVYVRGFDSNNVGVLINGIPVNGMEDGKVYWSNWAGLSDVTSNQQVQRGLG
                     ASKLALSSVGGTINIITRSTDAEKGGSVYAGIGNDGYMKQTFTVSTGMLDNGWAMTLS
                     GGHTYGNGYVQATNFEGWSYFVNVSKKINSKHSIVFNAFGAPQWHNQRSNKHTIQEFR
                     DHPMEAKWNSDFGYRNGDIYNIGYAYNYYHKPQISLNHYWKISDNAMLSTQAYASIAT
                     GGGRRVYGPNSTWLSLNYPSGTPYDNSALTPEGYFDFDWVIAQNAASTTGSKAIIANA
                     INDHQWYGVLSTFTTSISDFKITAGFDGRYYSGVHAYKIDDLLGGVYYLDNKNINRPA
                     SQPLHKGDYVNYNYRGQVLWLGLYAQAEYIKNSYSGFLSVAAASNNYRRVDFFQYAPG
                     NQKSPWHDFLPWNIKGGFNYKISESQNVFINGGYIKKAPIFSNSFLNNTNDFNKGVKY
                     EKIITAEVGYGFNLRNFNAKLNVFRTSWLDKGLVRSLNGQTANILGINALHQGVELEC
                     YYKPINTLNVKGMFSYGDYRWTDDVNFTLYNEDQSVAGTYNAYIADVHVGNSAQITAA
                     VGVDYEVLPKLKVGVDFNWFGKNYADFDPTARTTIESKVDAWEMPDYYLLDIDANYRF
                     KMGSFNATIYGNVSNLLDTEYIADATDGTYHDEFSSYVFYGFGRTWSAGLRINF"
     gene            complement(54342..57623)
                     /locus_tag="PK839_00235"
     CDS             complement(54342..57623)
                     /locus_tag="PK839_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015531242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HOG71191.1"
                     /translation="MKKIRFFIAMLFTVVGLWANAQVTTSEINGKVVDEKGMALPFAT
                     VVAIHEPSGTQYVTTTDNAGNYRLTNLRVGGPYKLSISFVGYSTGVYTDINLNLGESY
                     LQNVKLKESTTSLEEVVVSAGMGNPILSSERTGAQTNISRGDINTLPSISRSITDFSK
                     LTPQAQGNSFVGRDGRFNTITVDGAAFNNNFGLSSNPLPGGNAQPISLDAIDQVSVNI
                     APFDVRVSQFTGASINAVTRSGDNSFKGSAYSYIRPKSFTGDKVDGNDVEGANDRNSQ
                     NFGLSFGGPIIKDKLFFFINGEIENEDIPGVTWKPSTDGVANPDQMISRTTEADMIRV
                     RNHLISTYNYDPGKYKNFDPFQNKNTKILARVDWNISKNHKFTIRYNDVVGTSDVETN
                     FNSGPPNNARNSGRISSQSLAFSNSFYGFENTVRSITGELNSTFLTNISNKFLGSYTH
                     IQDSRTSPSKLFPFVDIWEDGDQYMSFGYELFSYNNDVINNTLSLLNNTTINLKKHTL
                     TLGVSFDRLYFKNSYIREGTTYYRYASVDDFINGADPIGFGVTYGYNGEDAPGAELTF
                     GMGAFYAQDEWKVNTNLKVTYGIRLELPFYFDDMKDNPAISALTFANSQKIDVGSWPD
                     PQLLFSPRLGFNWDVKGDRSLQVRGGTGIFTGLLPFVWFTNQPTNSGMIQSPEIGWGP
                     GNANLMGLQFNPNFKEFIASNSTLFPQTPGTLPNNAGLAEVSKDFKMPQVWRSNLAVD
                     VELPYNFVFTGEALFGKDINAVQQININEANPTGNMVGPDNRPFWATANDRKVVSTIA
                     NAVVLSNTDKGYQYSFTAQLTKNFSKGFSGMFAYTYTMAKDISSNPGSAAYSAYSSNT
                     AVGSLNNPGLSYSNFATPHQLIGHASYRIEYGGMFATTISLAYRGFQQGRWSYTYSND
                     LNNDGISSDLIYVPKDKDEALAMFVPYTPTGGVEMTAQQQADAFWAYVNGNDYLKDRK
                     GKYAERFGDVQPWLHRFDVKVLQDIFSNFGTSRRYTLQISLDLLNVGNMLNDKWGAYT
                     YNPLASYDNVRPLTRVALPTATTAPTFRLNAKTIEEFETKTTLSKNISTTSTWGALLG
                     IRLIF"
     gene            58109..58342
                     /locus_tag="PK839_00240"
     CDS             58109..58342
                     /locus_tag="PK839_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG71192.1"
                     /translation="MLGVLKYANSSPSYLFATALIASACRPQAPPIGRSLPATLIVPA
                     QPLPCHSAGSHQPTGSIAEVKFRFLTRRLPVVN"
     gene            complement(58583..60700)
                     /locus_tag="PK839_00245"
     CDS             complement(58583..60700)
                     /locus_tag="PK839_00245"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG71193.1"
                     /translation="MRKLISAILAILPMFTTFGQRSDIDSLLKQLNEAPNTSVKAETA
                     IDLAQRYINIDTDSAMAFNQIAHQLATDLSDTALMIRCENTYGFIFNVNSSFEKARER
                     FSRVLEMAISAHDSSLIASAYGNIGNTYMFEQKYSMAVESFLKALDIFEKRNNQKGVA
                     TVYGVLGNLYLSLDKLDQSLEYYQKAQSMFHTLGWADYEATALMNIGVIYKNKKDYKK
                     ALEYFSRAQKFETNQGNYYKSAQCLANIGEVEFALKNYEKSIAYQEKAFDAFEKVKAK
                     NDAALCLMSIGCAYDSLNKSAMAKSYYAKTLSYIDSLNQPNLKMTLAGQIYSLNKKLN
                     NFEEALKYYEIYTMWKDTILARDNQKNLAELLTKYETVQKEKEIELQKERVKRRDIIL
                     LSELLLSVLLIGLVGVLVSSYRRKKRDNEILEQSNSLISQQKEEIQTQRDEVVSQRDR
                     IIRQNRLITDSIEYARHIQSTILPSREQLSAFFPEFFIFYLPRDIVSGDFYWFKLQEN
                     AGILAFADCTGHGVPGAFMSILGITLLDDIVTPKSSISPSQILEELRVNVKNSLKQSG
                     KRVETKDGMDMAICRFNPASTYLEYSGANMPLTIIRNGEVLEFRPTRNPIGYYPKEMP
                     FENHQVELRKHDMLYLFTDGFSDQLGGPNYQKYKRQNLINLLKSIAGLSMTQQENILA
                     ASHSDWKGTNEQVDDILVMGIKF"
     gene            61007..62053
                     /locus_tag="PK839_00250"
     CDS             61007..62053
                     /locus_tag="PK839_00250"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOG71194.1"
                     /translation="MKKVTLNDIAKKVGVSKTLVSLVVNGKARGYGISGQTCDKVMAA
                     VRAYGYNPNQTARSLRTGMSNTLGLIVSDISNNFYASLARQLEDLAWELGYNMIIGST
                     DESVGKELKLLEIFRNRQVDGIILSSSQQNPTELETLVKSGFPLVLIDRYFPTVPIPS
                     AVVNNKVGGELLAQHLLNQGFRNPVVLSLTPSYISSVAERVEGFVSVFENAAIHTEVH
                     NIPFLCLVDMVEDFFTTRLHDKNMPDAVFAVNNNLATATLRACHVHNIVIPKDIALVC
                     FDELPYFDFSKPSITSIRQPVAELCSVAFSLLLGQITNNGETKPGHVEMMPVELLVRE
                     SSTQISDNNGWRSP"
     gene            62411..63559
                     /locus_tag="PK839_00255"
     CDS             62411..63559
                     /locus_tag="PK839_00255"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOG71195.1"
                     /translation="MNRKTFTILLAFLCMGFGDAVGPFVGLAKEQFELSNFEAQLIAF
                     MGFIMFGFLSVPMGIFQDRRGKKVVLMLGLAVALAGLTIPLFGLGSYALFLLTILLLG
                     AGAAILQVAGNPIMRDVSEPGMYSRNLSFGQFIKAIGSLSGSIIPFAAAKWMGADWQV
                     LFPIYVSALLLTLVAVGFTKVEEEKSDKKPATLNSCFSLLKNRFVLLMVLGIFIYVGI
                     EICLSSGVPILLSTHFGIDLKTWGLLGNAFFFIAILIGRLLGSIILSWTKPAAFFRVS
                     CLIALMGILLIFSNNQITTLVGIFTTGLGFANIFPLIFSITVDAMPERANEISGLMVT
                     AIAGGAFIPPVMGLVADMSSVRTGFLVPLLGLGFIIVLSFLKPQPSRQ"
     gene            63556..64683
                     /locus_tag="PK839_00260"
     CDS             63556..64683
                     /locus_tag="PK839_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOG71196.1"
                     /translation="MSYSDDSRIVLTLDAGGTNFVFSALQGIKPIADTITLPSNGNNL
                     EKCLQGIVEGFTKLGAKLKGKPSAISFAFPGPADYPKGIIGDLGNLPGFRGGVALGPM
                     LQEKFGIPVFINNDGDLFAFGEAIAGFLPWVNSMLKNAGSPKNFYNLLGVTIGTGLGG
                     GLVTNGKLFTGDNSAGTEVWLLRDGVNPTCFAEEEISIRAVQRTYHAHTRKNESTLLS
                     PKEIAEIAYGKHEGDMVAAKLAYQSLGASLGDALANIITITDSIVVVGGGLSNAYHLF
                     APAMLAQLNGSISTVTGEQVNRLESKVFDLENEGQCNLFVKGENRKIKVPLSSKEVDY
                     DPLKRVGIGVSRLGTSHAVAIGAYVYAINMLDHNTLHSQAI"
     gene            64889..67840
                     /locus_tag="PK839_00265"
     CDS             64889..67840
                     /locus_tag="PK839_00265"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HOG71197.1"
                     /translation="MKKPQLLTVVMLISIELFSQVKDPVDYVNPFIGTDFHGHTYPGA
                     TVPFGMVQLSPDTRLTGWDGCSGYHYSDRVIYGFSHTHLSGTGASDYGDILIAPVSGK
                     PVFENTRYSSPFKKENEMAVAGYYSVFLDKYKVQAELTATRRVGFHRYTFKNPNKAAL
                     VIDLEHRDRVIESWLKVVGKNELHGFRRSSHWANDQQLYFVIRFSQPFEKGPNFVDAS
                     EQLSITNGENGEVLAEGTRLKAWLQFPKLPNGKLLVKVGLSAVSEEGALRNLEAEVPD
                     WDFDSVAAQAKNDWNIELSKIKVDGGTPDQLTTFYTALYHAMVVPNVFSDVDGSYRAM
                     DNQVHHAEGFTPYTVFSLWDTYRAWHPLMTIIDTRRTTDYINTFLSDYRFGGRLPVWE
                     LAANETECMIGYHSVPVIADAWIKGIRNFDPDLALKAMLHSANLNHFGLKEYRQYGFI
                     PGDMEHESVSKTLEYAYDDWCIAQFAKSIADSSTYQKFIMRAQSYKNLFDPSTHFMRP
                     RINGGWKDNFNPSEVDQNFTEANSWQYSFYVPHDVSGLIELHGGKKNFIGRLDELFST
                     SSQLMGKNQVDITGLIGQYAHGNEPSHHMAYLYCYAGQPWKTQKLVRKIMDEMYTSKP
                     DGLCGNEDCGQMSAWYILSAAGFYPVCPGSTQLVIGSPLFSKITFNLENGKYFSVVAE
                     NNSPENIYINSASLNGKPITRCWIDYNEIMAGGELRLAMSPTPNSGWGSTDADMPTSS
                     IDEHFIATAPAFNTSTKTYMDSLQLTLTTVQPQASTFYSVAEINHPEAIPIWTQGNTL
                     NLKSSKRVKAYSVLPGGESSAIVEGSFIRIEGIKNVELKSIPSPLYDGGGKLALVDGQ
                     RGSTNFRLGGWQGYQNQDFEAIVDLGHERELHYLGAEFLQDASPWIMMPRQVTFSVSS
                     DGTSYSNIGVVNNDIPDTLEKPIVKELGVAVNVEARYVKVFAKSYGKLPSSHESAGES
                     SWLFIDEIVIR"
     gene            68035..68433
                     /locus_tag="PK839_00270"
     CDS             68035..68433
                     /locus_tag="PK839_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG71198.1"
                     /translation="MNNDFICPKCEGYLVVGGSVILSISMQSGLRGLILLSPELGNYA
                     KVSSPHVKIENGEMLDYYCPICHAKLAASDVHPRLVRLIMIDDKSKRHEIFFSGIVGE
                     HCTYKISETVYEKFGESSATYDQYFKTRRI"
     gene            68931..70304
                     /locus_tag="PK839_00275"
     CDS             68931..70304
                     /locus_tag="PK839_00275"
                     /inference="COORDINATES: protein motif:HMM:NF018583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HOG71199.1"
                     /translation="MITVIIPTLNEQNTISQVIELVEKSANVSEILVVDDKSLDSTVK
                     IARAKNVKIYTSTKLGKGSSMYEGMLLATNGIIVYLDADITTYPENIVNLLTDPIINN
                     TADFVKATFNRQAGRVTELVAKPLLSLLFPELAHFSQPLSGMIAAKKSILEKATFEND
                     YGVDVGLLIDMFQEKARIVEVNIGYIENRMQSWEQLAKMSKEVSRAILKRAELIKHSN
                     LETLGNFAMIRDQMDFAIKESLLGLNKMLIFDMDNTILTKSFILTLATNYKFKEKLIE
                     IVAENSSPYVRTKLIAQLLKGLKINELLETLDGIPTVNDFERIVKEFKQKGYICGIIS
                     DSYDCITNHLVNKFDLDFSIANELEFSNGMATGEVKIPSAFLRTKDSTCNHDFCKSNA
                     MREVAHRYHIDLHNIIAVGDDENDICMIKDCGIGVAFCSSNSTLNFIADRVIQERTFA
                     PLIELLK"
     regulatory      70556..70646
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            70767..73220
                     /locus_tag="PK839_00280"
     CDS             70767..73220
                     /locus_tag="PK839_00280"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOG71200.1"
                     /translation="MKKFFLLLLMQLAAAWAFGNPTDFSGSLKVSGRVTDSEGNPLVG
                     ATVALEGTLLGTSTNPDGSFVLNRLRPGTYVLIASFVGFETARLNLVLQENVVINITL
                     KESTLMFDEVVVSATRASGRMPIAQTTISKEQIDGNNTGFDVPYLLELQPSVVATSEG
                     GTGIGNTAIRIRGTDMSRINVTVNSVPLNDAESQGVFWVDLPDFTASVDNLQVQRGVG
                     TSTNGAGAFGATINFQTVSLSQTPFTSFDIFAGSYNTWKLSARFGTGLVKEKFSFEGR
                     YSQLGSDGYIDRAGSDHRSMFMTAAWHGAKSLIRVNIIHGEEHTGITWEGTPDYMLQI
                     NRRYNPAGEYTDEFGNTRYYKDQKDNYWQTHYQLISTFSLGSNLTLNATVHLTDGKGY
                     YEEFKEDRKFSKYALPNLVLGIDTIRTSDIIQRKWMENQFYGAVASVNYRKKSLEVAV
                     GGGWNRYDGDHFGRILWSAYNYGLPKDYEWYRNNGLKTDWNIFAKSVWQPGKMLSLFA
                     DIQLRGIQYDLKGPDSDLLPVDQEHSWHFVNPKIGTTFRFSPASELYVSFGVANREPS
                     RSDLKDALKGATGFAPKPERLYDWELGYGLRHQDFAINTNIYYMKYRDQLVLTGELTD
                     VGYALMDNVPDSYRAGVEILLGLKPFQWLNVEANTTFSRNIIEHYTNYVNLTDNPTDW
                     NETGLTEAEYLGNTTISYSPSVVGSAKITVTPVKALYFSWIGKYVGKQYIDNTESSSR
                     SLDAYFVNNIVGEYKFSPKMAKSISLQVVVNNLFNLKYEANAWVYRTKFQSGDPEYVS
                     IGYYPQATRNFMLRLAVGF"
     gene            73493..74116
                     /gene="pnuC"
                     /locus_tag="PK839_00285"
     CDS             73493..74116
                     /gene="pnuC"
                     /locus_tag="PK839_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HOG71201.1"
                     /translation="MSWLIGNYIELLGATIGLIYLYFEIKQKIWLWPLGIATSALYIY
                     IYFSSKFYADMGLQVYYLIISIYGWYHWLHGNVDNTSSSKPLPVTRITLRLLVVLTTI
                     SLLLFVILWFVLDRYTDSPVPVGDAFTTALSITATWMLTRKIIEHWWVWIVVDGVSMG
                     LYIYKGLYPTTVLFAFYTILSVVGYYQWKREITPSQVKTTAGSSAKE"
     gene            74151..74765
                     /locus_tag="PK839_00290"
     CDS             74151..74765
                     /locus_tag="PK839_00290"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HOG71202.1"
                     /translation="MNAVIVANGKFPEHPKPLRLIHEAKTLLCCDGSIEHLVKLGITP
                     TAIVGDLDSVSPELRERFKNILFHDPDQNSNDLTKAVSWCAERNINSISIVGATGKRE
                     DHTVGNIGLLVRYARMGLQVEMVTDTGTIKPLLESAKLTSFPGQQISIFSPVNQTTIT
                     TNNLKYPIDNGQIPEWWMGTLNESLGSWFELNFSPGPLLVYQMY"
     gene            74885..75961
                     /locus_tag="PK839_00295"
     CDS             74885..75961
                     /locus_tag="PK839_00295"
                     /inference="COORDINATES: protein motif:HMM:NF012336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HOG71203.1"
                     /translation="MKYLNSVITGTGSYIPSVKISNREFARNTFFKKDESAIETLVDE
                     VIAKFKDITGIEERRWVTDDLCASHIAAIAAEEAIATAGIDRETIDQIIVSHNFGDVL
                     QGTIQSDTIPSVASRVKHLLKIKNPACIPYDVIFGCPGWVQGVIQADSYIKSGLAKRC
                     LVIGAETLSRVIDKHDRDCMIFADGAGATIVEAVEADEKAGVIASSMMSHTEQEVFYL
                     SLGKSFHPDSDSRIRYIKMDGRKIYEYSLINVPGAMREAIEKSGINPSELKKIIIHQA
                     NEKMDEAIVARFIKQLKIQTPISDLMPMSIKELGNSSVATIPTLYDLILKGKVPNHTI
                     AKGDIIMFASVGAGMNINAIVYRQ"
     gene            complement(76049..77632)
                     /locus_tag="PK839_00300"
     CDS             complement(76049..77632)
                     /locus_tag="PK839_00300"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF006006.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HOG71204.1"
                     /translation="MEKLTKIAACLAGLVILIAVATGCHEREQAKVGETEYLEEITIK
                     QLQQGYREGKFTVAQVVNDYLTRIEELDKNGPVLNSIIAINPEAMRIAQELDSELAAG
                     KSRGPLHGIPVILKDNIESKDSMPTTAGAVVLKNSYVWRDSHVAKKLREAGAVIIAKA
                     NLSEWANFRASLSSSGWSGVGGQTRNPYDTERNPCGSSSGSGVAVAANLCAFAIGTET
                     NGSIVCPSNNNGIVGLKPTVGLISRTGIIPISFTQDTPGPMARTVEDVAVCLGIMVGV
                     DSTDAKTLNPDAVFYSDYSKFLKRDGLKGKRIGLLTNVSGFHFKVDTLMKQSVDAMKQ
                     AGAEVVAIDFPVSREVDNASFEVLLYEFKDGLNRYFASLGESARVKNISELIKFNLND
                     SVELMYFDQQLLEMAQSKGDLNSPEYKKNLEMMQTGMRKQGIDRIMNEYKLDALVAPT
                     GSPAWKTDLVDGDHFLGGSSSYAAIAGYPSISVPMGFIDGLPVNISFIGKAWSEAELL
                     SIAYSYEQATHQRKKPMLE"
     gene            complement(77880..78767)
                     /locus_tag="PK839_00305"
     CDS             complement(77880..78767)
                     /locus_tag="PK839_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG71205.1"
                     /translation="MENNEKSAKTIKTLRVIMVMLLLIIAGLTVVYFQQKQQAKDTQE
                     ILQAQKDSISNNLLRLMNDYSELETTNDTLNQKLQKEQEHAKKLYAELQSVKQISYAK
                     IKEYQKELGTLRSIMKDMLHDIDSLNALNKELIAENVKVKEEAATAKQTVIELEQKTE
                     ELNTQVAKGSIVKARDIVAMGVNQKGNEVTRARRVNKIRACFTLSENSIAKPGTRNVY
                     MRIVGPDDFVLTKSEADLFDFEGEKIVFSASREVDYQNSDLDVCIFFDNNGELLTGVY
                     KVTLYMDGYMIGYSEFSLK"
     gene            complement(78807..79730)
                     /locus_tag="PK839_00310"
     CDS             complement(78807..79730)
                     /locus_tag="PK839_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOG71206.1"
                     /translation="MSIGISHITKQFGEQKALNDVSFNISTGQVVGLLGPNGAGKSTL
                     MRIITGYLFQDSGSVDINGHNTQDTRFDFRREIGYLPENNPLYTDMYVKEYLSWVASI
                     YKLGKEKRRRLSELIGITGIGDEQGKKIGSLSKGYRQRVGLAQALIHNPSVLVLDEPT
                     SGLDPNQIVEIRNVIEEIGRDKTVIFSTHILQEVEAICSRIIIINKGLVVADGTNTEV
                     KALSSGLRQNIVVEFAEKVSREAIMAIEGVETATLIDTNCWWITSGRLEDLRGKIFNF
                     AKNAGFTLLTLQKRDLQLEEVFQNLTLGNRN"
     gene            complement(79791..81062)
                     /gene="metK"
                     /locus_tag="PK839_00315"
     CDS             complement(79791..81062)
                     /gene="metK"
                     /locus_tag="PK839_00315"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HOG71207.1"
                     /translation="MGYLFTSESVSEGHPDKIADQISDALLDEFLRRDSEAKVACETL
                     VTTGLVVLSGEVKTKAYVDVQEVARRVIKRIGYTNDQYRFDSESCGVISTIHEQSPDI
                     TRGVVRQSEEEQGAGDQGMMFGYACRETDDYMPLALVLSHVLLMELAGIRREGRQMTY
                     LRPDAKSQVTLEYEDNNQPVRIHTIVVSTQHDEFDTDDARMLSRIYEDVKNILIPRAK
                     KRFPERVQKLFKDDYILHVNPTGKFVIGGPHGDTGLTGRKIIVDTYGGKGAHGGGAFS
                     GKDSSKVDRSGAYAARHIAKNMVAAGIADEVLIQVAYAIGVARPINLYVNTYGTAKVS
                     LSDGEIAQKILTIFDLRPAAIVKRLGLKNPIFEETAAYGHFGRDHYKRNVDFIENSNC
                     NGEKIVKREVEFFTWEKLDYVDRLKSEFGIK"
     gene            complement(81319..81717)
                     /locus_tag="PK839_00320"
     CDS             complement(81319..81717)
                     /locus_tag="PK839_00320"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HOG71208.1"
                     /translation="MMAKALKFFSKNRNSILWGLVILILCTMPPSGFPKVSVSGIDKL
                     VHFGLFAVLTLLVISENNTLRLSGSVLQKTRWVGIVVSIIYGGLVELLQLKVFTYRGA
                     DWFDLIADVVGTLIAAWLYIPISRLTRRLV"
     gene            82054..83442
                     /locus_tag="PK839_00325"
     CDS             82054..83442
                     /locus_tag="PK839_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016269978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HOG71209.1"
                     /translation="MFDKQIYVDRRLKLRKKVKSGIVLILGNTESPINYQGNTYNFRQ
                     DSNFLYFFGLDTPGLAGVIDIEEGTDCLYGDDFDIDDIIWMGPQPKLKDMGEKVGVLS
                     THPFRELSTTLNRAIRQGRKIHFLPPYRADNTLLLEKLLGINQTIIKTYSSIELIKAI
                     VSLRSVKEPCEVEEIEKACSVGYKMHVTAMKMAKPGALEREIAGELEGIAHSFGYMTS
                     FPTILSQHGETLHNHDHSLTLEEGRLLLVDAGAEGISHYASDFTRTFPVGGHFSQKQK
                     DIYSIVLAANNKTAELAKPGVTYFNVHKAAARVIVEGLSALGIMKGNSDEAVENGAHA
                     LFMPHGLGHMMGLDVHDMEDLGEDFVGYDDEISRSEQFGTSSLRMARKLQKGFVLTNE
                     PGIYFIPALIEKWKSEKINQAFINYDVLSSYLTFGGIRLEDDLLITDNGCRILGHRIP
                     ITPEEVEKTMNS"
     gene            83973..84953
                     /gene="nadA"
                     /locus_tag="PK839_00330"
     CDS             83973..84953
                     /gene="nadA"
                     /locus_tag="PK839_00330"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008126055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HOG71210.1"
                     /translation="MNYSIVDKLGYIDEDIDSNIDLKIAIRKIKEEKNAVILAHYYQR
                     PEVQDIADFVGDSLALSLKAASLNSSLILFAGVKFMAETVKILRPNSQVLIPDLNAGC
                     SLADSCQFSDFKKFIDLHPNHIVVTYVNSSAEVKTISDICCTSSNAIKVVKSLNNSTP
                     VIFAPDINLGSYVKSTLNRSNMVLWNGSCHIHNQFMLEDIIALKAKHPNAKVIAHPEC
                     QNSILLISDFIGSTKSLLDFTYNSNNTDFIVVTEPGIIHQMKKLSPQKNFYFPYVQNE
                     ECYCNECSYMKLNNLKKIYLTLKYEYPIINLEPDVISNAAIPINRMLKIN"
     gene            84962..85957
                     /gene="ruvB"
                     /locus_tag="PK839_00335"
     CDS             84962..85957
                     /gene="ruvB"
                     /locus_tag="PK839_00335"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HOG71211.1"
                     /translation="MENQISDKEFENNIRPSEFKQFNGQDDIIKNLKIFVQAAKIRSE
                     SLDHVLLHGPPGLGKTTLAYIIANELGVSIKTTSGPVLEKPGDLAGILTNLDKFDILF
                     IDEIHRLSPIVEEYLYSAMEDFRIDILIDKGPSARSIQIELNPFTLVGATTRSGLLTS
                     PLRARFGINFHLEYYDASTIKSIIKRSSYILNIDIDDDASMEIALRSRGTPRIANSLL
                     RRVRDFAQVVGNGRINLDIAKSSLDALKIDSLGLDEMDNKILTTIINKFNGGPVGINT
                     IATAISEDPGTIEEVYEPFLIKEGLLKRTPRGRQTTDLAYSHLKIEKNFRQGTLF"
     gene            complement(85949..86590)
                     /locus_tag="PK839_00340"
     CDS             complement(85949..86590)
                     /locus_tag="PK839_00340"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOG71212.1"
                     /translation="MTTEESEQLEANLECYEYKKGEIIYKEGSRITGFYCVKKGVFKI
                     YKTGIDSKPQIVAFAQKGDITGYRSVLSNEPACTTVESLEDSEVCYIPSSLIFEFIRK
                     NSNFALSLIQLTCKELNTANDLIKDIAQKTVRERLAETLLMLDQTFGKDEEGFINVNL
                     TREDISSIIGTATESVIRILSEFKTDGLISLKGKKIGIGNYSKLESISNSFLK"
     gene            complement(86679..87698)
                     /gene="pheS"
                     /locus_tag="PK839_00345"
     CDS             complement(86679..87698)
                     /gene="pheS"
                     /locus_tag="PK839_00345"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOG71213.1"
                     /translation="MQEKINNILELIDKFTPQNYEEVESFRISLIGKKGELTKLFDDL
                     KNLGTDEKKYYGKLLNDLKKKLNEKLDILKDTYKSKGKKEDKIDLTLQEPQLNFGTLH
                     PITIVKNEIFNIFRKIGFSIYEGQEIEDDWHVFSALNFPPEHPARDMQDTFFIQKNPD
                     VLLRTHTSSVQVRSMENEKLPIRVICPGRVFRNEAISSRAHCIFHQVEGLYIDKTVSF
                     ADLKQTLEFFVKEMFGSKTSIRLRPSYFPFTEPSAELDISCNLCGGKGCSVCKHTGWL
                     EILGCGMVDPNVLELNNIDSKKYSGFAFGMGIERIAMLKFRVNDLRNYFENDIRFLNQ
                     FTNFY"
     gene            complement(87718..88530)
                     /locus_tag="PK839_00350"
     CDS             complement(87718..88530)
                     /locus_tag="PK839_00350"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOG71214.1"
                     /translation="MNKNILLYFSIAALLLLTGCKNATVDNRHLVKISTNYGDIEVVL
                     YNETPKHTLNFINLTDQHFYDGQIFHRVIKNFVIQAGDPETKNSQPGKEYGEKDSGYL
                     IDPEFCDTIIHKYGAIGMARESDDINPDKLSSGSQFYIVIGKVYTNEELDNLEKKINF
                     EKLNKIKQDLYKKYLNENTAVSDKNIKDILILIENMVDSVKSSEKPFIFTNKQREIYT
                     TIGGIPHLDGNYTVFGEVSKGMDIVKKISEVKTDQNDRPLVDIVINEVKKIR"
     gene            88653..90146
                     /locus_tag="PK839_00355"
     CDS             88653..90146
                     /locus_tag="PK839_00355"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOG71215.1"
                     /translation="MKYFNFVRQILFMASVRQLYSQSVVYGLSTVVPRLLNYLLVPLH
                     TTVLTNPEQYGVITEMYAYISFILILLTFGLETGFFRFSSKFKNNESVYSTIFYFLIF
                     TSSLFFLIISIFSFDISNFLGSFYKPIYITTLAGVVSIDAVLALPFAKLRNENKPFLF
                     SSLKILGVVINVFCNILFYLIIPRNSLYTLFPNVDLLYFVFLSNLIQNIFVLILLLIF
                     TKIPKLIFSPKLLKSILIYSLPLLIAGLSGTTNEAFDRIFIKYLYPIKDEALYQLGIY
                     GANVKLAVFLVLFIQMYRFAAEPFFFKLQNESNNKEIFAKSFKYFLIFCFFITILITF
                     NIPILKYFVGSNYRHNLYIVPILLIANSLFGIYFNLSFWFKLSDKTLYGLKYVGIGAF
                     TTIISNVILIPLIGIFGAAVSRIISYTIMTLLCYIDGKRSGYISIDRNNLKIFLLFLL
                     FISFISTVLLIYSKPLFSLIFANLFLILFIFFFLKKENIKILNLRKC"
     gene            90140..90574
                     /gene="dut"
                     /locus_tag="PK839_00360"
     CDS             90140..90574
                     /gene="dut"
                     /locus_tag="PK839_00360"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004330378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HOG71216.1"
                     /translation="MLKVKIVNKSSFPLPGYATSHSSGIDLYANISSSIKILSLQRIL
                     IPTGIFVEIPDGYEAQIRPRSGLALKHGLTVLNTPGTIDSDYRGEISIILINLGSDEF
                     ILEPGIKIAQMVFSRYERVHFIEVNELTETSRGAGGFGHTGI"
     gene            90650..91831
                     /locus_tag="PK839_00365"
     CDS             90650..91831
                     /locus_tag="PK839_00365"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOG71217.1"
                     /translation="MKYIFKYCLFFILCYCITFPIKSQSLEDLNSTKRKIENQIQQLN
                     VLINENANLKSETINNLNLVSQRIKLKNSLINQLELEILYLNNNILQSQSNIDSLSFL
                     LNSRKKELEKILQFYQKYHNSYNILIFILSSNSFNQSFLRIKFYNSIIKYYSNTIKSI
                     STDINKISEIKVSLENQKKELYLKQLQKIDEIKTLRKDLDLYNSKLKKLKSNEKNLRK
                     QIEIEKRRSNEIISKINELIEEETRKKIISTNKSNENVKLSNDFLKNYGKLPHPVANA
                     FVINNFGETNHPVLKGVKIKNNGVDFACKTGSEIRSIFNGEVKKVFNLPMNGIAVIIR
                     HGNYLSVYSNLTNVYVKVGDKVITEQVIGKIVSNDNSGSILHFEIWNEKNPENPLMWI
                     Y"
     gene            91891..92286
                     /locus_tag="PK839_00370"
     CDS             91891..92286
                     /locus_tag="PK839_00370"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG71218.1"
                     /translation="MQTIIELNSSLDSISKVEKFIDEISANVNISSEIYGKILIATIE
                     AVNNAIVHGNKLNSNKIVRVKIESEGNRLKVIVEDEGPGFDFYNLPDPTTPQNIENIS
                     GRGVFLMRKLSDEINFFNNGSLVELIFYL"
     gene            92286..92708
                     /gene="ybeY"
                     /locus_tag="PK839_00375"
     CDS             92286..92708
                     /gene="ybeY"
                     /locus_tag="PK839_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HOG71219.1"
                     /translation="MAISFQSNINFNLSNKRKVKTWIKNVVTKYGFKIGDVTILFTDD
                     EYIKELNIKYLNHHYFTDILTFDYSSGLVLNGDIVISIDTVRSNSILYSTSIDDELLR
                     VIIHGFLHLLGFNDKSSEESRKMRELEDGALNIFYSND"
     gene            92701..94572
                     /gene="mnmG"
                     /locus_tag="PK839_00380"
     CDS             92701..94572
                     /gene="mnmG"
                     /locus_tag="PK839_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019151899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HOG71220.1"
                     /translation="MTDFFDIIVIGGGHAGCEAASIAAKLGSSVLLISLDLTKLAQMS
                     CNPSIGGIGKGQIVKEIDVLGGYTAKVTDLTTIQFRMLNKSKGPAMWSPRAQCDRMLF
                     SYEWRKYLESIDNLHLFQDEVVDIDFDVKNQLVSGVTTQSGIKFKCNVLILTNGTFLK
                     GKIHIGSNNFSGGRFGEKASIMLSDKLEDMGLHIGRMKTGTPVRIDGRSVDFSKLLEQ
                     KPDTYGKFSLDPKYTSTLQQKSCYVAHTNSVVHDIIREGFDKSPLFSGKIKGIGPRYC
                     PSIEDKVRTFYTKDSHQLFLEPEGVNTYEMYLNGFSSSLPVDIQLKALKNIEGFENVS
                     ILRPGYAIEYDYFDPMQLNHTLAHKSIKNLFFAGQVNGTTGYEEAAGQGLLAGINAHY
                     FINDSSKQLILGRNEAYIGVLIDDLVTKGVDEPYRMFTSRAEYRILIRQDNVDERLLP
                     IAQKLKLASNEDIDRFNVKSKKLESFKNLISSITVSPQDVNDYLVKVNSSPIEHKRKL
                     NDILARPDVNFFELLDSCPNLIGILGDVDKNIAENYIIKIKYSSYEEREQFMVDKTQK
                     LEALKIPNNFNYESIRSLSTESRQKLSKVKPKTIGQAKNIPGVSPSDINVLLVYLGR"
     gene            94814..96430
                     /gene="ade"
                     /locus_tag="PK839_00385"
     CDS             94814..96430
                     /gene="ade"
                     /locus_tag="PK839_00385"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HOG71221.1"
                     /translation="MNRSGIVKGVVYNPILHESFGALVFIENGKILKIERDGNIKEPL
                     ILPGFIDSHVHIESSMLTPAQFGRTAAAHGTVAAVADPHEIANVAGVEGINFMLSSAI
                     ESPIKLFFGAPSCVPASPFDECFQPFSPGIIKELIDRSDIFFLGEMMNFPGVIGGNAE
                     VMEKINIAKSAGKPIDGHAPSLDKNDLKKYISAGISTDHESFSLEEALDKLSFGMKVI
                     IREGSAAKNFDALHSLIPRFHDNLMFCTDDCHPDDLIVGHINKLVKKAINAGYNIFDI
                     LQVSSVNPVMHYNLNVGLLQPGDPADFIVVDGLSSLNIISTYINGDDVLKEPRPTTNV
                     KIPTYTFPNSFDSNLLVKKLDTKRVKVIDVVKDELITNWHVENSNTDLLEANPKEDLL
                     KIAVVSRYKKNEASVGLIRGFGINKGAIAASIAHDSHHIIAIGCDDNSITQSINYIVK
                     CGGGICYFDGDSIYGLPLPAFGLMTNESAEVAGKAYHELTQRVIADGCKLQAPFMTMS
                     FMSLSVIPHLKITPTSLFDVDKFSFTNICL"
     gene            96466..98262
                     /gene="uvrC"
                     /locus_tag="PK839_00390"
     CDS             96466..98262
                     /gene="uvrC"
                     /locus_tag="PK839_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HOG71222.1"
                     /translation="MNDILSNIVTNLPDSPGVYQFFDINGILIYIGKAKNLKKRVSSY
                     FHQSAYESAKLRVLVSKICDIKFIVVDSESDALLLENILIKKHKPRYNVLLKDDKTYP
                     WIVIKNEPFPRVYKTRKYIDDGSSYFGPYTSGRLVFELLDLIRKLYPVRTCSLNLTTA
                     NILAKKFKPCLEYHIGNCLAPCIGMQSHQSYLQNIDSIKRILQGNLSEIKNHLKAEMV
                     KYSNQYKYEEAQLIKEKIDILTNFQSKTTIVNPKYKDIEVFTILTRHNIVGVNFIRIN
                     KGQVVQSYNLHLKSFVNESLPELFSSAIAEIRQRLKANSTRILVNIMPEFTINGVSFS
                     VPQRGDRKKFVELSLRNLEIFLDDLYKNEAAKSPVLAVDSILTKIKDDLNLSDLPVHM
                     ECFDNSNIQGSNPVASCVVFKNAKPSKKDYRHYNIKTVQGPDDFASMKEVVYRRYSRM
                     LSDGETLPQLIVIDGGKGQLNAAMESIVELGIQNRVHLIGIAKRLEEIFVPGDPVPVY
                     LDKRSETLRIIQQIRNEAHRFGIKFHRNKRSNSAFESSLEQIEGIGPKTIQLLFTRYL
                     SLDGIRNAPESELIDLIGTKRTNLIKNYFKKV"
     gene            98329..99174
                     /locus_tag="PK839_00395"
     CDS             98329..99174
                     /locus_tag="PK839_00395"
                     /inference="COORDINATES: protein motif:HMM:NF025459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4271 domain-containing protein"
                     /protein_id="HOG71223.1"
                     /translation="MDSLNLRINLYDNLKQAFFSPDTLNGGCTISPSADTALASSTKL
                     NNVVSSLSGDANVMVEPILRKTNNIPNSYIIPFLAGLVVLVVIVLRYRKYLASIVESA
                     LYSFFAEKFVLETNVPFRRTFALLDALSVVSFSCFSLVALKLLNTEFFYESKPIILIA
                     IAIASFSLFRLFISIYNYLINSILSNNPIFSLFQYNYFIFIRIVGFIILPISFVAFYA
                     PTSVGLYLLYFMFGILLVTLFYRLYRLTLIFVKNRISILYFILYLCGLEVVPILIVIK
                     EVQRI"
     gene            99199..99951
                     /locus_tag="PK839_00400"
     CDS             99199..99951
                     /locus_tag="PK839_00400"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HOG71224.1"
                     /translation="MKISRIVVAQQEPVNEKSPYLDISNRYGVKIDFRPFIHVEGISA
                     KDFRAQKINISEHTAVIFTSKTAIDHYFRMCEELRVTIPETMKYFCITESVALYLQKY
                     IVYRKRKVFFGNNTFPDLLEIIQKHKEEYFLVPLSDIHKPEIPKALEKAKLKFRKGVF
                     YKTVSSDLSDLNPFPYDMVVFYSPSAVKALFDNFPQFKQNDVVIGGFGKNTAKAIKDA
                     GLRLDISAPTTTCTSMTLAIDNFLKNLSKKSK"
     gene            99977..100381
                     /locus_tag="PK839_00405"
     CDS             99977..100381
                     /locus_tag="PK839_00405"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HOG71225.1"
                     /translation="MKYFLRKNNRLTLQHQVDLLFSHGYGYFIYPIRAKVLLYDRNVE
                     EDTWFKVMVVVPKRHVKKSSSRNLIKRRIKESFRVAIHSLKGSCPSNKIILIAISFVS
                     GDIKPYDRIKVSIEKLMNEIENDIEKLKSIKD"
     gene            100448..100684
                     /gene="yidD"
                     /locus_tag="PK839_00410"
     CDS             100448..100684
                     /gene="yidD"
                     /locus_tag="PK839_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009437163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HOG71226.1"
                     /translation="MNKNKIRDVLILLPLGLIKFYQLFISPHIPNSCRYTPTCSVYSV
                     EALKKHGLFKGSWLSIKRILRCNPWGGHGYDPVP"
     gene            complement(100686..101837)
                     /locus_tag="PK839_00415"
     CDS             complement(100686..101837)
                     /locus_tag="PK839_00415"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOG71227.1"
                     /translation="MSISGNTLKNFALIIFAGFIVIFSLLVSNKLVKNLSEEEHAKID
                     LWAKGVRFLSTTQDSCQDLTFVSEVIQNNRTLPVLLTDESGRIISHLNISERQIKDSV
                     AVYRLLRKMTSEHEPIEIMLSDSTKNYVYYRDSTTLRRLEFFPYIQIVIILLFFLISY
                     YAYSQSRKAHENSIWYGMAKETAHQLGTPTSSLMGCLALLEEKIPNDPIIMELEKDIV
                     RLEKIAGRFSKIGSTPILQEANLIELLNSTIDYLGARVLKNVTVVKKYNVQEKLNIRM
                     NGILIEWVIENIVKNSADAMMGKGEITFKVTDNTQVVFIDISDTGRGIQKGKFEEIFR
                     AGFTTKDRGWGLGLTLSKRIIEQHHGGKIFVLNSEPGVGTTFRIVLKKR"
     gene            complement(101849..104029)
                     /locus_tag="PK839_00420"
     CDS             complement(101849..104029)
                     /locus_tag="PK839_00420"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HOG71228.1"
                     /translation="MSAQNLDLKINAARLLLGNFEINSCKAENGRLNLKTNLLKKNDI
                     NPANGQAALLQNEFFINRFTLSSFKALIEDTLSRQKSLIGIDRLTLNIRAKKILTLNL
                     DCQLKEINLGSKFMLNEQTQVNAIASYDGKSVFLRNVKISLSGSRLELQGLYRLENST
                     LGLGFKLNRTNINRLIQVANIKGLPADITGKVAMSGKLSYNGGKKNLTGLWIDYDAQN
                     VSIIAKGKIIEIKEINGLSYFTNNLQTHSGQVSNAVVRYENIEAAFSGRTYGTNRIII
                     LAEGLLKLGKNEPSFTNKLTGLEGNGTFKALLELRKGNGLQSKHFGFLLHDVASEISF
                     SLDSISAFKNLSNISGKATIRDKAIISATCYLDSSKIDFTLCQNNAIYYLNNPLNFSP
                     SIYLNSSYLNLDSLIKSVSTVNKGGKKRVYTTVETSINTKKLIYTNYAFENVTGVMNI
                     VGDSIYVKGLNAKGFEGKIDGDLMVVGDKYSINASMEGIDITELFRHYNSWNQKYITH
                     NNIEGHLSGHCNFDFELDSANNIRWDRAKMNSEITINDGKLRGMNNIKKLSKWLKLDQ
                     VKSIDFTTIHNNILISNGCIYIPSMDLNSNVMGLNLSGKHFLTNDYEYHARINFSSLL
                     AQRFLNYSADYNQPKTSSGAINLYLTLKGNSKGYEVFLDKKSSYEAIKNTINTEGQSL
                     KEIISNEFKQTFEDSLKKQHAIQQQPTSQKFQVEWDEYDTTKTK"
     gene            104973..107180
                     /locus_tag="PK839_00425"
     CDS             104973..107180
                     /locus_tag="PK839_00425"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG71229.1"
                     /translation="MKFRIVSLCLLFAFRVGISQNIDSLLLKLYQESNDRSRIEILLN
                     ISSFYDQHDRNFIQAENYLYEALQIAQTAGDNTLLANTYNELGKFYRNQSKYPEALKY
                     HHLADSLAVKMGDCILQATTLNNIGVVYRRLDNHAQASEYHLRALNIAEGCKDTFNIS
                     VACNSLGNIFSLNGRYQQALSYFNRALALSISQNNLLGQAMNFNNIGEVYEFMGDYRN
                     AKYYYTKSLDINFKLKNLKGISINYNALGKIEMFNGNPENAYRLFQNALDIDRKLGDK
                     KFLADSYVNLAKACLALKKIDKAKIYLDSTITIAQQIGSPWHLQSAYEAYSDYFVQKS
                     DYLSALLSYKNASRFKDSLLNEKNARHLSTIQTIYETEKKEKEISYLLQEQALKQKEL
                     EKNNAQKNALIIGLVLSSLVVIVSLFAWRTKVKSNIVLSDQIKEIEDKNRLLEIQKEE
                     IEQQKIKIESNQAFIEQKNKNLEEAHKIIESYIENITDSIRYAEKIHESIQPSLSDML
                     SIFLDAFIYSKPKDIVSGDFCWLTRRDNLVFLALADCTGHGVPGAFMSTIGINILNQA
                     INEQHLYSPDKILTFINRTLINRLNKSTGEQVLKNSMDIAVCTFDTESLLLSYSGALI
                     PIFIQKGNSVEEVKPDSITLGTNFSHSASGFSLQNIQLNHGDWVYLSSDGFFDQLGGE
                     SRKKFTRKQFMQIVADSPNMKGNVKVDIIEKKFLQWMNRTPQLDDVLVWGFQV"
     gene            107215..107904
                     /locus_tag="PK839_00430"
     CDS             107215..107904
                     /locus_tag="PK839_00430"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOG71230.1"
                     /translation="MKIPDTTSLQLKISNTHTINMNENRSIERKRFCISLFVSLIFIV
                     ALWCIKIFEIVEGISLYRLGVFPRQISGLPGIIFSPLIHSDFNHLISNSLSLLFLLIG
                     LLYFYRDLSYKVILFIWITSGLMVWVGARDSYHIGASGLIYGLASFLFFSGVVRHDQR
                     LMAISLIVVFFYGGLIWGILPVYPAISWEYHLFGGISGLIAAILFRNQGPPRKVWSWE
                     QEEDENTPENL"
     gene            108196..109146
                     /locus_tag="PK839_00435"
     CDS             108196..109146
                     /locus_tag="PK839_00435"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HOG71231.1"
                     /translation="MESHIIINQLLAFIVLAVIGAIASWRKIINDEGKDFLSRLIIDV
                     TLPLLIVSTFLKLESNPRLLVNGLWLFVLTFVNIGVLYMAGTLSARAQKLSPSDSTVH
                     VLHTMFGNIVFLGFPLFDALFPNGVGVFYASVYQLASNFITYTYGTYKLSAGVQKSKL
                     GNFFNINTIALLLGILLMVLKVKLPAFILEPISSLGKCTSPLSMVYIGALLAGMNLKS
                     TFKTTSIYVLTINKLIIVPLLLGFIYSFLRRSFNLNLSIEAFVVLVMQAAMPCQTIVV
                     VMSRRYGGSHVLATGNLFISTIFSIISLPLVYHFLVSIYS"
     gene            109281..109736
                     /gene="dtd"
                     /locus_tag="PK839_00440"
     CDS             109281..109736
                     /gene="dtd"
                     /locus_tag="PK839_00440"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HOG71232.1"
                     /translation="MRVVIQRVKSAKVEVNNHEVGCIHKGLLVLCGFEVEESEEDLQW
                     MASKICKLRIFDDETETMNLSIKDISGEILVVSQFTLHASTKKGCRPSYVRAAPSSLA
                     ILFYNRFLNLLEIELGSKIAAGVFGKMMEVSLVNSGPVTIVIDSKNKEF"
     gene            109739..110065
                     /locus_tag="PK839_00445"
     CDS             109739..110065
                     /locus_tag="PK839_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HOG71233.1"
                     /translation="MTFEEAQKEVDSWIRDHGVRYFSELTNTVLLMEEVGELARIIAR
                     KFGEQSFKDNESADGLSDEMADVLFVLICLANQTGVSLSEAFEKNIEKKTQRDSQRHK
                     NNPKLF"
     gene            110104..110979
                     /gene="deoC"
                     /locus_tag="PK839_00450"
     CDS             110104..110979
                     /gene="deoC"
                     /locus_tag="PK839_00450"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HOG71234.1"
                     /translation="MDFFVEDKFNLEVNGEEVGRHLARIKDLALSRLGDTDKLKACFG
                     SIDLTTLNTTDTAERGKQFAEKVNGFTVDFPEMPSVAAICVYPTLVSSVKENLKAKGV
                     KIASVAAGFPSSQTFLEIKLSECRMAIGQGADEIDVVIPVGSFLQGDLYSVFSEVKQI
                     KETAGDIHVKVILETGALPSLKEIKLASFLAMEAGADFIKTSTGKLEPAATPEAVFVM
                     CTAIKEYFEKTNRKVGIKPAGGVVTSSEALVYYTIVEQVLGSSWLSPDLFRLGASRLA
                     NNLLSDIYGRAVSYF"
     gene            complement(111082..111984)
                     /locus_tag="PK839_00455"
     CDS             complement(111082..111984)
                     /locus_tag="PK839_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005867990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOG71235.1"
                     /translation="MKPTLIILAAGMGSRYGGLKQVDGLGPYGETIIDYSVYDAIRAG
                     FGKVIFVIRRSIEEDFMKIFGNRFSRDVNFDIVFQELNMLPDNITCPPEREKPWGTAH
                     AVWVARKMVNEPFAVINADDFYGYDTFKTLANELSRSNLKNGQYFMVGFRLNNTLSEQ
                     GSVSRGACEVDNASFLKSIVEFTRIQRVDGKIFNIDDSGNRIELDGSMLVSMNFWGFT
                     QDFFTLTETLLKEFLNGIPDHKKSEIYIPTVVNRAIQDGLATCKVLSTTSEWFGVTYP
                     GDKIHVVDQLRKLTLNGVYPSPLW"
     gene            112238..113485
                     /locus_tag="PK839_00460"
     CDS             112238..113485
                     /locus_tag="PK839_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008823384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HOG71236.1"
                     /translation="MMRRSDIEVMAPAGNFESLMAAIQGGADAVYFGVGKLNMRAKSS
                     VNFLEDDLYEIVRICSASNVKTYLTVNTVLYDEDIDDMHRLIDTAKKARVSAVIVSDQ
                     AAMEYARQVGVEVHLSTQLNISNTHSLRFYSQWADVVVLARELNLQQVKLISEGIHKH
                     NINGPSGNKVRLELFVHGALCMAISGKCYLSLHQKNYSANRGECLQICRRSYTVTDNE
                     TGSQLEIDNEYIMSPKDLCTISFLDQILDAGIRVLKIEGRARGPEYVKTVTSCYAEAV
                     EAIVSGQYTPDKVEGWTKRLTSVFNRGFWDGYYLGRRLGEWSNSYGNIATERKQYVGK
                     ISNYFSDMGVAELNVEATELYLNDKVLIIGPTTGVIETLVSEIRVDNQQASFAPKGLP
                     CSIPVPSKVRRSDKLYRIIYSKP"
     gene            113752..114375
                     /locus_tag="PK839_00465"
     CDS             113752..114375
                     /locus_tag="PK839_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4254 domain-containing protein"
                     /protein_id="HOG71237.1"
                     /translation="MVKNFSELCNNIFDECTKYYHTMDNVDANPNVPYKSNTIEHSLF
                     IKNWIDAVQWHLEDLIRDPNINPEKALLLKRRIDKSNQDRTDLVEQIDSYFLEYFKDV
                     RPQNGATINTESPAWAVDRLSILALKIYHMEQEVSRTNASADHVATCRQKLTILLEQR
                     KDLSMAIDQLLDDIANGRKYMKVYRQMKMYNDPSLNPILYGKNETNP"
     gene            114350..115426
                     /locus_tag="PK839_00470"
     CDS             114350..115426
                     /locus_tag="PK839_00470"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HOG71238.1"
                     /translation="MARMKPTLKENKPKKLLVIRLSAIGDVAMTVPVLWSVVKHNPDV
                     RVTFVSQSFARDIVSQIPGIQFYEADTKNRHKGFWGIFKLFRELKNLDRFDAVADLHN
                     VIRSRVIGVLFRIGGIRYEVIDKGRKEKKQLCSVKNKKLVELKPTTERYADVFRHLNL
                     VVSSDFDYLFDEARLPDDISAYLGYKSGTWIGVAPFAKHKGKIYPLNLMEAVVSRLAA
                     NKSYKIVLIGGRNFEKELLEEWAKKYTNTFSVAGKYSILNELKIISNLDVLISMDSAN
                     MHLASLVNTPVVSIWGATHRFAGFYGWKQNADNTVEVELSCRPCSVYGNKPCFRKDYA
                     CLNRITPEMIISKVNSVVDRYGRI"
     gene            116036..116947
                     /locus_tag="PK839_00475"
     CDS             116036..116947
                     /locus_tag="PK839_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HOG71239.1"
                     /translation="MSYVTKEKLFSKAWFKAYTLITIGAFVLAAGFVLFIDPHRIAPG
                     GVYGIGIIVHYLTQGVFSWAPEGFPIGTVGLLLNIPLTILGIKILGPRFGVKTVVGFI
                     LSSVFIDTLHMIYGYHPLVDDMLLSSIFGGVLIGFGLGLIFKSKATSGGSDIIAMIIA
                     KYTRLPLGQLMIYVDSTIVLIALVAFKDWKIPLYSWVVIYITGKVIDMTIEGASYDKA
                     LFIISDRYEEVRSKIINDLERGGTLISVKGMYSGEEKKLIFTNVSRREVFMLKDYIRE
                     IDPKAFITVIDANEILGEGFKPISEAL"
     gene            117126..117209
                     /locus_tag="PK839_00480"
     tRNA            117126..117209
                     /locus_tag="PK839_00480"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:117160..117162,aa:Leu,seq:caa)
     gene            117317..117766
                     /locus_tag="PK839_00485"
     CDS             117317..117766
                     /locus_tag="PK839_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018695246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase family protein"
                     /protein_id="HOG71240.1"
                     /translation="MNKTTSFNDPKQLQFDRRYLEMAKIWAKNSYCKRRQVGALIVKN
                     RMIISDGYNGTPSGFENICEDENGITKPYVLHAEANAITKVAKSGNSSEGATLYVTSS
                     PCMECAKLIIQAGIKRVVFCDEYHNPDGLSLLSRAGIDVVKLDIEEL"
     gene            117848..119521
                     /locus_tag="PK839_00490"
     CDS             117848..119521
                     /locus_tag="PK839_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOG71241.1"
                     /translation="MAYQNSKKDIYYPIVIALVLAAGILIGQRFNGKSQKPGTLVYNR
                     TDKLSSVISYIEEEYVDSISVSTLVENTIPAILKNLDPHSIYIPASELQSVSEPLEGG
                     FDGIGIQFNMSNDTAVVVNTIKGGPSERLGILAGDRIVKVNGKLIAGVKFPMDSIPIL
                     LKGPTGTKVKVEIKRKDVDELIDFEITRDKIPISSVDIAYMVTPEIGYIKITTFAKTT
                     YKEFLDAMAKLESQGMKKLILDLRSNGGGYMDAATKIANEFLKPNQLIVYTQGKSRTR
                     QDVYSTGTGKYQDIPLQVLIDEFSASASEIFAGAIQDNDRGQIVGRRSFGKGLVQEQI
                     FFSDGSALRLTIARYYTPSGRCIQKPYTPGDDNYFNDITSRYLHGEFEVADSIQFADS
                     LKYYTVGGRTVYGGGGIMPDIFVPLDTNGITPYLQQVSRRNLIYRFAFNFTDQHRKET
                     RGLKDFTSVDRFLNKFDLLSDFIKFAEQNGVPRNEKEISISKKILDAQVKATIARNII
                     DNEGFYPYIRNIDETLLKAIGLMEQSVSQPTTSALHRSVNSRVLNQAQL"
     gene            complement(119512..120312)
                     /locus_tag="PK839_00495"
     CDS             complement(119512..120312)
                     /locus_tag="PK839_00495"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOG71242.1"
                     /translation="MEDYVELPICTIRYRLSGQGPAVILVHGYLENLEIWEDIVPDLE
                     KNYTVLRVDLPGHGKSACNHQTISMDLMADSISAVMTTLDIKKAFMVGHSMGGYTTLA
                     FAERYPNWLNGFCLLHSSANADSSEKRQSRMNDIETVKNGGKRDLVYLSIPRLFANQN
                     LEKMDDIVDKFIKIGLSTPDQGIIGALNGMAKRPDRNYVIENANIPVFFIFGKHDNLI
                     SMEVAEALMERHKKARTVILNNSGHMGFVEEKDQTTAAITSFLTQVYS"
     gene            complement(120543..>121158)
                     /locus_tag="PK839_00500"
     CDS             complement(120543..>121158)
                     /locus_tag="PK839_00500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG71243.1"
                     /translation="TGQGLYQKSWLVDVSAGTPLLEKGGTMPIQARISGGYEIQFTES
                     GILGLAIRGGYASTRGHRSLTDETGVVYDGGESLYSYTGNSLFVGVSPMIIFEQYDSN
                     FDRLFIELELGLASHNVDMKFYEPTKTSTSNWVHFNGYFALKAGFRLAWKKEEGYFRA
                     MSIWGSLHNQSPINAIEKSIPKNWPYEFKEDLKSQWQIGISVYI"
BASE COUNT        35123 a        25878 c        24809 g        35248 t
ORIGIN      
        1 cggcggagcg ccggagggcg ggatttactc cggaacgggc gttaccggtg gaacccttga
       61 ccccgcagca gctggtgccg gaacgcatac tatcacctac tcctacaccg atgccaatgg
      121 atgctctgca agtgcttccg aggatatggt ggtgaacggc ctaccaactg ccaacattaa
      181 cacaacagac ccaaccgatt ggtgcgaggg tactcctata aatgtaaatt taacggccga
      241 tgttgccgaa tcctaccagt ggttgctaga tgggcaaccc attacgggcg aaaccgggca
      301 aacgctgatt gccacacagg cagggactta cagcgtagct gctactacta atggttgtaa
      361 ctcaactggt aacacaatta ccataaccgt tatatcaaca ccctcagtgg ccattactac
      421 tgaggatccg acaacatggt gtgccggtga tgaggtttcg gttcagctta cagcaatacc
      481 ctcgagtggc gatagctacc ggtggattaa ggacgaggta accattgctg gtgaaacaca
      541 gtccaactac acggcaaatg agccaggcaa ctatagggtt gaagccttat ttgccggggg
      601 atgcagctcc gtttcggaaa ttttctccct aaccgagaac cccgttccaa cggttagcat
      661 agctaccgat accattcaga ttgacacctc atcaacctac acatttgatg cgggggcagg
      721 attcgtatca tacctttgga gcgatacctc taccggacaa acccttctgg tagatggagc
      781 cgttaccgga acaggtgagt tcacatactg ggtaaccgta accaacgagt ttagctgttc
      841 agcttccgac acggcagtcg tggttgtttc acctgtgggt ggcgttccta tgcaaatttc
      901 gtgggaaatt tcaatctacc ccaacccgtc atcgggtgag ttcaagctga aggtttcagg
      961 cattgaaccc ggaacataca cccttacaat tttaaatgcc ggtggtcaaa ttgtctatgg
     1021 atcaaccttt agctcaagtg atacagaaat cacacgggca attaacattc aaaccctatc
     1081 taaaggattg tactacgtaa gatttgaaaa cacgaataag ggatttaccc gaaaaataat
     1141 tctgaaataa tagctttact tgaatttata gataaaagga gggttcattt accctccttt
     1201 tacatttaac cttgatcttg ctggctattg attgaagaaa atagctcctc gtagctatcg
     1261 gccacagctt cccagctgta taggttttta atcctagtat aattcgactg cttagccttt
     1321 aaaacagaca tggcaggaag acggccaaac tccctaatcc tgtcattcaa ctccgtggtg
     1381 ctgccgaaat accacccttc atcaccaaga acctcccggt taaaagggtt gtcatgcgct
     1441 ataacaaaac atttacaggc cattgcctcc agcaacgagg ggttagtgcc tccaaccgta
     1501 tgcccatgta tataggccaa tgaatggtaa cgcaaggcat tcaaatcatc ctggttaaaa
     1561 tttggaccta aaaaacgaat attcttgaaa tgttcaaatt ttctatataa actttcacca
     1621 aaacgagtgt cgtacctgcc aataataatc agattttcgc ctagagcaac aaaagagcga
     1681 gcaatcatct cgatgttgtt ttcgggggtc agccttgaaa ccataaggta gtagggttca
     1741 aagattccgt acattgccaa tacatcccgt gggggaatct ctccaagcgg aaatgcaccg
     1801 tagggtatga ctttaggctt aacgccatag gaagcggcgt agtaggacac cagggcttgg
     1861 ttatcacaaa ccaaccgggc tacaaagtgc actgccatat actctgaaaa cctaaggaat
     1921 agctttgcgg gtttgctcca tttagacctt ttatgctcaa gcccatcgag atttacaaca
     1981 atcttttgtc taaacgtttt tttaaaaagg ggtagccagg gagcaaatga atggccacat
     2041 tccagaataa catcaatttc ttcagtattt gcccagcata gcgattttag gtcgtaatga
     2101 agggtttgga agttgtgggg aagatgtttc cctacaccta cattaaccag gaacaacatg
     2161 gagcttagcc tggtaacgct tctacctttt ccattaaaag ttggagaaag aacgtagaca
     2221 aggtgtccac gagctaccag aatttgagac agcacctctg caaaacgttc aaaaccgcca
     2281 tagctgttag gtattcccct agttccaagt attgcaattt tcacaagaaa gtggattacg
     2341 gataaattaa aatcctatac tgccgccatt attcttcctg ccaaccatat tttaagaaga
     2401 gaaagagacc cctgtaacta taagctgtca acaagcatca actaaaacaa atgatacaat
     2461 tgttttagct aaccttaaaa ccatttcaca agccaaatca actgcattgt gcgggtaatc
     2521 tatcttatag ctaaaggatt ggggtagcaa aaacttttct atgatttgtt ttcaaaccgc
     2581 gggactgtga agaagaatgt ggatcccttc ccttcggtgc tctcaactgc gatggttcca
     2641 cgattttttt caacaaattc cttacataaa atgagcccaa gcccggttcc ttcctcctca
     2701 tttgttcccg gagttgacgg atttgaatca atcctaaaaa gcttttgctg attttcggga
     2761 gatattccaa caccggaatc cctaaccatc actttaaggt attcggaatc cttgacaacc
     2821 tcaacctcta ttctcccctt agctggggta aactttatgg cattgctgat gaggttgcgt
     2881 aaaaccgtgg taatcatatc cctatcgcaa taaacaaaat gatcgtccgg attaacccaa
     2941 gctaactcta tccccttctg ctgagcgttg gccttaagca gatctatatt ttcatttatg
     3001 atttcagcca tattaagcga tttcaagttg acaaccgttc ggccagtttg aagcagcgac
     3061 cattgaagca ggttttcgag aagattaaag gtgttgttgg agtacttata tatctgctga
     3121 ataaacatgt ggagcttttc gggttcaaaa ctttcaaact cccttgcaag cagctcagaa
     3181 aggctaagca cagcaccaaa gggatttttt aaatcatggg caattattgc aaagaacttg
     3241 tctttagtct gattaagtga aacaagctcc tttcgctgca catccatctc ttccttttgc
     3301 cttttaattt cctgattttt agctgtaagc tccgcttcaa taacattgta atcgcgtaac
     3361 cgccacacca gggtcttgag caacgctctc gataaaaggg gattcttgtc aagcagcagg
     3421 ttgaattgcc cctggtccag cctaagtaat tctgtatccc tgagtgcggt aatagttgtt
     3481 gacctggcta aagaatcgag catagagtac tcaccaaaaa actgtcgggg gccaaaagtg
     3541 gcaaatgtat ggttgccgtc atgagctttc acctgtccac taacaactat ataaagggca
     3601 aaatcatagt cccccttgtt gaaaaggact tcgcccgcct ttatgctttt tgaaactaaa
     3661 cttttggcta ttttgcccaa cccctcgcta tcaacaccca tgaatagggg tattgatctg
     3721 agaagtgatt cgtaacctgc atataacgac tcgtcctttc ccctattaaa aactttaaac
     3781 agcatactga ataatttatg aacaaatata atgtatatta tttatacata gaggtttaca
     3841 actaatattt tttaccatac ctgataaata ctggctctta aaaccattca taatgaattt
     3901 accatcaaat atcatcaata aatatctaaa gagtattacg taattataac ctaatatgtt
     3961 gaattttatt aatattgtga ttgtatttat tgcttttgaa aaggggaatt gttttgaccg
     4021 gcatcacatg aaaacctata gaaacagcac ggttgttatt atgttaactt aaaagaggaa
     4081 cctgaaacct ggaccaaaat gtgaaaaaat acttgggcat acatgctgca ctcgattatg
     4141 gtagagagct aaaaacaatg tgtgcctata tgcaatttat ttaaccttat aatccttcta
     4201 agcaggtggt atgactatta gtttacaatg ttgtaggtag aaaaattata tttacgacca
     4261 aaccagggcc gatggttgca gcatgttgcc attaataata ttgacatatt gctatataac
     4321 aagaaaataa atagaagcag atgaaactta agctaaacat tcgccaaaaa atactggttt
     4381 acattctagg tccatcgata gtagtatttg ctggcatatt cacatatact acccggacct
     4441 caagggatat tgcttacaac caggcaatca agctaaccga tagctatgcg catcagtatg
     4501 ccttgaacat tgaaggatgg cttaaccagg atttagcgat tacccgtaca ctggccgcag
     4561 cttttctgga atacaagcag atgccgtttg agcaatggca acggttgata tacccaatgt
     4621 acgacagaat aatcctaacc actccgcata ttgacgccta ctgggatagc tgggaaatga
     4681 gcaatcttga ccccacatgg gacaaaccct acggccggta cttttacatt gtttacaagg
     4741 aaaacggggt ttacaaaacc aaagcagaac ttagaagtct tacgggcgac cctgatacct
     4801 atggagccat gaagaaagcg gcaaaagaaa ttatcaacga accctatgtc tcggctctac
     4861 aaagaggcca gctgatgaca accatttcgt cgccacttgt tgaaaatgga aaatttatcg
     4921 gactaatcgg tattgacttg ctgctcacca gatttcaaaa tttggttaac ggcattaaac
     4981 cctaccccaa cagctatgcc ttcctgcttt caaacaaagg aaactttata gcccaccccg
     5041 attcaacaat ttatcaaaaa aatattgccg aacgccttcc ggaactgaat agtagattca
     5101 gcattctttc caacgttcag aaaggaaagg cgtttagctt cacccaaacc ggtgaaaatg
     5161 gccaacgtct ttactacaca ttctcgccta tcaacattgg caatacacaa actccatggt
     5221 ctgttggcat agcggtacct atcaacgata ttctttacga agccaacaaa aactacaaca
     5281 ttagtattgc catcctgatt ttaggcttga ttcttcttat agcaattata tttttcgttt
     5341 cgagcagtat taccagacca attaagagca ttacccatct gctccaggat ttagctaacg
     5401 gtaaggttga tagcaccctg gcaatcaagc acgacgtcca tgacgaaatt gggaaaatgg
     5461 cgctagcgtt aagcgagagc attaagggaa taaatgacaa aacagaattt gcaagagaaa
     5521 tcggttcggg taacctcaag gcagaggttt cgcttctaag cgaggatgac caccttggaa
     5581 aatccctttt agagatgcgt gataacctgc aaaaagcaaa ggaggacgaa gaaaaacgca
     5641 aggctgaaga cgagaaaaag aggtggacca acgaggggtt ggcaaaattt ggcgatatac
     5701 tccgccaaaa caacaacaac ctatccaaac taagcgacga aattatcaaa aaccttgttt
     5761 ggtacctcaa cgcttcgctg ggcggaatat ttgttctcaa cgagaatagg gaaaccgggg
     5821 ataaaacatt cgatctggtt gctgcttttg catacgaccg caagcgcatt cttaacaagt
     5881 cttaccagtt tgccgaaggg ctagttgggg cctgcgctgc cgaaaaggac atcatcatgt
     5941 taaccgaaat accccaggag tatatcgaaa taacctccgg acttggcggg actaacccaa
     6001 acttcctgct ccttactcct ttggttaacg aggatgaggt tatgggagtt attgaaattg
     6061 cctcgcttag aaggtttgaa gagcatgaaa ttctttttgt gaaagaaata gcgaagagta
     6121 ttgctaccac actacactcg gttaaggtaa actcgctaac agccgaactc cttatgaaat
     6181 ccaaggaaca ggctgaaatc atggcagctc aggaagagga gatgcgacag aacatggagg
     6241 aactacaggc cacccaggag gaagctgcca gaaaaacttt tgagcttgag ggtttggcca
     6301 acgccctaag cgcttcatcc ctgataatgg agtacgaccc tgcaggagta atcaccagca
     6361 ttaacgaaaa gtactccgag ctactaagca tttccaggga ggatataatc aacaaacatt
     6421 actccgacat agctaccgag gcaatagata aggaaaagct ggataaactc tggcaggaga
     6481 ttcgctccgg aaaaaccaaa tatagaaaag tcgtactcaa tataaaaggt aaagagcata
     6541 ccctggtcga aacctacacg ccaatcctcg acacgcaggg tcggctttac aaggtgttaa
     6601 aaatagcaac cgatataacc aacctataga aaataaaggg ctgttgaaaa tgcagccctt
     6661 tccattttaa ttctatgctg atttagcccc agcgcagaaa atgctgctgt caataaattt
     6721 ggcattcaat atcataaacg attttgcata acatagctcc ctccttgcta tagccatctt
     6781 ggcatcctca ttctggcaga agatgacgac tcgatagaat gtacttcact tttctaccaa
     6841 ttattttttg gaccgccttc gggtagttta gaaataacag gcaatttcca caattaggta
     6901 actctggttt ctcttatcat aaaatattta aaaaataatt tatctatgta ttgcatagta
     6961 gtaattttag tactatattt gcatcgtatt aataccataa tttacaaggt ataaattttg
     7021 ccatcgtatg aaaaaaactc taacagtaag cctaaacggc aggtcgtacg agctggagga
     7081 agatgccttc aaaatgctcg atgagtacct taagcagctg gaatcctact ttgccgagga
     7141 tgccgggaaa caggaaatat taagcgacat agaatcgcga attgcagacc actttgacga
     7201 atccaacagg gttcggggcg tggttatagg ggcagatgag gtgaggcggg taattgaaat
     7261 tatgggaacg cctagcgata ttgccgatgg agaaaaagtg ccgaaagcag aaaagcccca
     7321 taggagctac cgtaagctct accgcaacat ggacgataag gttctgggcg gtgtgtgctc
     7381 gggcatggga tgctactgga atgtggagcc ccttctcttc cgaatcatct tcatcatttc
     7441 aatcttctgg ggtgggcttg gtcttttggt gtacgcaatt ttatggatag ctgtacctcc
     7501 ggctctaacc acaacccaaa agcttgaaat gcgtggtgag gaggttaccg ccgagaatat
     7561 tggtaaatcg tttgaaaaca aaaaataacc ctacggatat ggaagtcgaa aatgccaagg
     7621 cgcagatgcg caaaggaata ctggagctat gcatactctc ggtgctttca aaaggtgatg
     7681 cctacgccaa cgatcttata accaagttaa aagaagccaa aatgattgtg gttgagggaa
     7741 ccctctatcc actgcttacc cggcagaaaa acttgggatt acttagctac cgctgggagg
     7801 aatcgccaca ggggccaccg cgcaagtact actccctcac cgacaagggg agaacttacc
     7861 taaacgagct gcatcgcagc tgggacgagc tggtggagac agtgaataca cttcgaaact
     7921 aataataaca agaaatacct gccgtaactt ttaaccttaa cctatgatgc tgaacttcaa
     7981 cacgacactt aatcgtccat actttcgcca gcatgtcata ccgacgataa aaagtagccg
     8041 taattcagct ttcgtttagg tgaggtgagg cagaagttta gcccaaggat ggtttaacca
     8101 accattttac cataaaaaca ctactttgtt gccaaccgta aagtaaatta aagcaaatga
     8161 aaaaaacagt aagagtgagc ctttcgggct tttcgtataa cctggaagaa gatgcataca
     8221 ggctgctcga ccattatctt gaagaaatca gggtgaggtt gggttcaaat gctgacgccg
     8281 aagaaacctt aagggatatt gaggagcgga tttccgagct gcttactgcc cgaatgggcc
     8341 agcgtgagac tgttccggcc gacatggtgc aggatattat caaccagatt ggtatgcccg
     8401 aggaagttac cggaaatgct gaatacagta aagatcagtg ggagactcag ccacccaaaa
     8461 agaggcttta ccgtgatact gaaaaccctg taattgcagg tgtttgctcg ggcgttgctg
     8521 ccttcttcgg ggttgatcca attgtaatca gaatcatttt tattgcgcta ctgtttgcca
     8581 gagggtttgg gctgctcctg tacatcattc tctggattgt tgttcccaga gcgctaacac
     8641 ctaagcagaa actcgaaatg aggggacaac cggttacgct gtcgaatatg ggcaaaaata
     8701 tttccgagga gatgagcagc gttaagagct ccgttagcca aaaggttcca aggaactttt
     8761 ttgaacgtct tggaaagttt ctgggtcagg taggcttctg gctgttgaaa tttatcatgg
     8821 ttctcgtaaa ggttgtagcg attgccatag gcattgtgct gatagtttcc atgctctttg
     8881 ctttcattgt actggtggga gcattcttct ttagcggaat taccctgccc tggctgttcc
     8941 ccggaacagt tgtaagctcg ctaaacgagt ttatcacctc gatgattgac ataagtagcg
     9001 gcctatggat cactattccc accttcctta ttctagccat tcccattgtc gccctaatct
     9061 acataggcat tcgggttttg ttcaggttca aggcacgtga cgggaaaatt gcgattattg
     9121 caacagggat ctgggtcgcc tccgtgctaa cccttgccat cagcattttc attcaactac
     9181 gaagcctaag tttttccgga gcagacaggc aggttgtgca gctgagcagc cagctgccca
     9241 gaaaccaaca aaccatctac ctgaaagcat tcccgcaata cgatgaagca acgctaccct
     9301 cggtatataa attcttcgac tattccataa caacagtaca aggggaaaag gtaatttccg
     9361 ggcaaccgaa gctggttatt gaaaaatcgg attccgatag tatcagccta attttatcaa
     9421 aaaatgcacg cggattcagc agcacaaatg cagctaaaaa tgcagcggat atcatttacc
     9481 catactcggt gaaagattcc accatatttg ttgacagccg gtttactcta ccagcttcgg
     9541 ttacgtggaa gggtcaaaca ctaactttat cccttttact tccggaagga tacagcattt
     9601 accttgactc tagcatttgc ggcatcctgg acaccgacca accctacagc agccactggc
     9661 ccgatgaaat ggtcgggcaa acctggacta tgacccgcaa tggattacgt gtaaaaagat
     9721 aggtagcaat tgcccatacg atgagttgtt gcttaattgc tcctggtgaa ggctgcgtgt
     9781 attagataca acaatgttat gctatgcgca aagactacct ttgcccataa tgagatggcg
     9841 aatgaatagc caaaattttt tggcgggtga taaaaaatcc attacttttg ccccactata
     9901 actaggggta ttgacctatg gtgtaatggt agcacagcag attttggttc tgttagtcta
     9961 ggttcgaatc ctggtaggtc aactttaaaa gcattcctat gaaaacggaa tgcttttatt
    10021 tttaagccga gctggaggca atcctcaaat ttccctgtca aagcccttga gcagggctat
    10081 ttcctcgggc cacttcgatt cgtcgggagt ttcgaggata agcggaatac cgtcgaaacg
    10141 gggatcgccc attagcattc ggaatggttc agcacctagc aaaccaagac caatattctc
    10201 atggcggtca accctgcttc caagcccctt tttggaatcg tttaggtgaa ctcccctcaa
    10261 gtagccaaac ccgataatcc tatcaaattc agaaaatgtt ttggtaaatg cctcgggggt
    10321 aagcaggtcg tatcctgccg taaatgcgtg gcaggtatca atacaaacgc caatgcgggt
    10381 tttgtcatca accctgtcga tgatgtaccg cagctgttcg aaggtgaacc ccaggttgct
    10441 cccctgcccc gatgtattct cgataaccgc cgtaacgccg tgcgttttat ccagtgccat
    10501 gtttatagac tcggcaatca ggttgaggca ttcctccggg gtagtcttat ttaggtggct
    10561 tcctgggtgg aagttgagcc tgtcaagacc cagcagctca caccttgcca tctcgtcagt
    10621 aaaggcattt ctggatttct ctagcgcatc cctgtcgggg tttcctaaat ttatcaagta
    10681 gctatcgtgg ggtaaaacct gtgcagggct gtagctaaac tcctcacaac gcctacgaaa
    10741 caaatctatg ctcttgggcg ataagggggg ggcaacccat tgcctttggt ttttggtaaa
    10801 cagcgcaaag gcagtagctc caatggcgtt ggcgtttacc ggtgcatttt ccaccccgcc
    10861 tgctgcgcta acgtgtgctc ctacaaattt catatcctga tggattttaa cataagcaat
    10921 ctgaagaaat gcctttacca aatacctgtt ttctccccca ccctaaaaac ctcaggttga
    10981 ttaccatttc catgggaagc ttgctgtcat cggactaaag gccagaacat cctgatcatc
    11041 aaaagtcgaa aatatctgac aatttaacaa acttcgtcga cgaacgttca ctatatttgt
    11101 aaaaatgatt accgtaatgc ggtaacggct aacttttcta caatgccagc aattcgtcta
    11161 accaaagagt ttcgctttga gatggcccat gttcttgagg gttacgatgg accatgccgg
    11221 aacatacatg ggcactccta taagctaaaa gtaacagtaa tcggcaaccc catcagccaa
    11281 cccggacacc ccaagctggg gatggttata gactttggcg atctgaagca agttgttgaa
    11341 accctcatta ccagccgttt cgaccattca attgcggttt tggaaaattc caacattcat
    11401 aggaagctcg ctggctccga cctcgagaaa gttgaggctt taccttttca gcccacctgc
    11461 gagaacatgg tggagtattt tgcccgcctt ctggcaccca gcctacccga aggcgtttcc
    11521 cttcaccacc tcttgctgca cgaaacggct acctcttacg ccgaatggta tgccgatgat
    11581 aacccttgaa ccgcttccgg tgaaaaaagc atttcgtttg tttcatctgt tctgttagag
    11641 caagcagcaa gcttgctcca agtgtcctat taggttaacg ctataactaa attcctcgcc
    11701 cgacgaggtt gggcgtagta ttgcgaagca cacctgcgtc cagtttttca ggcagtgcct
    11761 cagtttgtgc attcaggctt aacagccttt tctcgctcgt cgtaagctgc cggaggatag
    11821 gatcgagacc gcataagttt caacttttat ctgcatgatt ttggcaaaat ttgctaactt
    11881 gcgtctgtgt tatacggtca ggagaccgga attcatggac aacgaagtcg caggcttcgt
    11941 cgagcggtgg ggacaaaaga acggggtatg aaaagtataa tccttgataa tattgaaagc
    12001 ataaaagcat tatgtttaag atacaatgtc aaatccttgt atgcttttgg ctctgtttgc
    12061 acagaaaaat tcaatgacaa cagtgatatc gatttattaa tttccttcta tccaatggat
    12121 tatggcgact atgccgattc gtattttgaa ttggctgaaa agctggagga tgtatttcaa
    12181 cgtccagttg acttggttac agataaatca ttagggaatc catattttat tgaatcaatt
    12241 aatcaaacca aaaccccaat atatgaggct tgaaataaaa aagttcctgt tcgatataaa
    12301 gaatcaatag aatcaatcga aagctatctt ggggatgaaa gagatttcag catctacatt
    12361 gaaaacaaaa tgcttaggag agccgttgaa agagagtttg aaattattgg agaagcaatg
    12421 aatcaaatta acaagctaga ttcaacgatt aacatttctg gcaaatatca aattatcaat
    12481 atgcgaaatc gggtaattca tggttatgat aaaattgaca atgagataat ttggggaaca
    12541 atagtaaggc acctaccaaa attaaagcaa gaagtcgaag ccttattaaa ataaaaagca
    12601 accggttaca acatacggtt caatctacca gggtcattaa tacagacact aaacttttta
    12661 gtagcctagg agggatctta aagtggaata tcgtcactgg tttttaactt cccggcaatt
    12721 tccagccagc ctcgaaactg tacctaaaat tagataatat cgggttgggc gtagtattgc
    12781 gaagcacacc tgcgtccagt ttttcaggca gtgcctcagt ttgtgcattc aggcttaaca
    12841 gccttttctc gctcgtcgta agctgccggc gaactaacta cagtgggcag aacagtcagt
    12901 ataacttatt tgcgcagtta tcctggtacg ataaggattc ggagtaagcc ggatgcgggg
    12961 taggcaggtt gacaagttta atcatacaag gcaatgcttg ttgcattaat tgtgtttgta
    13021 cactactatc tgttattgcc gttgtaggtg aaatgacatt ttaaaatttc gtacgggaaa
    13081 gaaatgccgt ggtggatatg ctatggcatc cctgcattta ttacatacgc tttgccacca
    13141 attgcactac gcatgacaag gcgagagttg ctattttata ttccatatca ttgctgatgg
    13201 ctccaactat acatgtattc ctttcatttt tctttggttg gcatgaatta atgccacttt
    13261 tctacgtgcc ttatgctttt gaattattat gagactcgaa aacgctattc caatatacga
    13321 gcataaaaat gaccatttcg ctgcggtttc tgtcgttttg accaccccgc tgttcaattc
    13381 aaacctcgca ttttttcaga aactcctgtt gcgtcatcac ctaaaaggtg tacctttgct
    13441 cttgggttag ccgcttcggc ctaagcacat caagtgaaaa agggtagcac ttacaactaa
    13501 aacccagtaa gaatacacca atacggatga aaaaaaaact cctgctaatt gatgcctacg
    13561 cactgattta ccggtcatac tacgctttta ttaagcgacc aattagaacc agcaaaggcc
    13621 ttaacacctc cgccatttac gggtttaccc gagccgtgct tgatgccatc agaagattta
    13681 agccaacgca tgttgccgta gcttttgacg tatcgggacc cacctttcgg gacgaaatat
    13741 atcctgacta taaggcccaa cgacagcaaa caccagagga tataaagatt tcagtcccca
    13801 tcataaagca gctgctggac gccttgcaaa tagcggtagt tgagttgccg ggctatgagg
    13861 cagacgatgt ggttggcacc cttgccaccc aggccaatcc cgatgagttt gacgtgatta
    13921 tgctgacacc cgacaaggat tatggccagc tgcttcgtaa aaatgtttct attgccaagc
    13981 ccggccgaag cggaaatgat atgcagcttg taaccgccga tgagttctgc cgcatgtacg
    14041 acattcaaaa tcccaaacag tttgttgata ttctggcgct ctggggcgat gtctccgata
    14101 acatcaaggg agttgaaaaa gttggcgaaa aaattgccgc caaactcatt tccgactttg
    14161 gcagcattga taacattctg aataacacct caaagctaaa cgactcgcta cgcaaaaaaa
    14221 ttgaagattc acgtgaaaaa cttgcactca acaagaagct ggttaccatt gttaccgatc
    14281 tcgacctaaa ggttgatatt gacaaggact tggtgagcag ggaacccaac accaacgaac
    14341 tcctgaagct atttcaggaa ctggaattca ggtcgctaat cagggaattc gaccggcaac
    14401 cggtagccgc aaaaaaggag tatattcagg gtagtctgtt tggggaagat ccagagcaaa
    14461 aggttaccgt aatccaaaac cttacaacca taaaagatat aaaggcagat tacacagccg
    14521 ttacaacagc agaggagagg aagcttgtgg tagaggaaat ttcggggtgt accgaatttg
    14581 ccttcgacac cgaaacaacc gggttagatc ctatctccga tagcctggtt ggcctgtcgg
    14641 ttgccacaaa acccctaaag gcatggtgga tacccgttcc tgacgatcgt gagtcggtta
    14701 gcaaaatttt agccgagttc aaacccctgt ttgaaaatcc ccaagtggca aaaattgggc
    14761 agaatataaa atttgacatg caaatgctgg ccaactatgg aatttccgtc aagggagaac
    14821 tctacgacac catgctggcc cactacctgc tcgatcctga ctcgcggcat aacctgaacc
    14881 acctgtcgga aatctacctc ggttacaagc ctattgaaat tgaggagctt atcgggaaaa
    14941 aaggctcaaa gcaggcaaac atgcgaaacg tccccctgga gaaaatcacc ccatacgcct
    15001 gcgaggatgc cgacattgcc ctacagctga aattgaggct tttcgccgaa cttgagtcgc
    15061 agggtcttac gaaactctac ttcctactcg aggcaccatt ggttgaagtg cttgctcaaa
    15121 tggagcgcaa tggtgtttgc ctagacgtgc aaagcctaca ggagtatgga aagattctta
    15181 actccgaatt gcttaagctg gatgccgaaa ttaaggccat ggcgggtgtc cccgacctca
    15241 acatctcctc gcccaaacag ctaggcgagg tgcttttcga caaacttcgg attgctactg
    15301 atgtgaaaat gaccaagacc aagcagtact ccaccagcga ggaggagctg ctaaagtacc
    15361 agagcgccca tccaattgtc ggaaaaatac tggagtttcg ctccatcaaa aagctcctgt
    15421 ccacttacat cgaaacgctt cccgggttgg tgaacacgaa aaccgggaga attcatacct
    15481 cgttcaacca ggcggttgcc gcaaccggac gcctaagctc caacaacccc aacctgcaaa
    15541 acatacccat acgcgaggag aatggccgtg agatacggag agccttcatc ccatcaccgg
    15601 gtagctattc catgctctcg gccgactatt cccaaattga gcttaggctt atggcccaca
    15661 tgagcggcga ttcggccatg atggaggctt ttcgaaacgg cgaggatatt catgccgcaa
    15721 cggctgccaa aatattcaag gttccccttg aacaggtgag ccgtgagcaa cggagccggg
    15781 caaaaacagc caacttcgga atgatttacg gcatctcggc ctttggtctc tcccagcggc
    15841 tcggcatctc gcgcaccgaa gcaaaggagc tgattgacga gtactttcga acctactccg
    15901 gggttaagcg ctacatggac agctgcattg cccaggcacg cgaacggggc tgggttgaaa
    15961 ccctttttgg ccgcaagcgc tatctaaacg acattaaatc gagcaaccag acagttcgcg
    16021 gaatggccga acgtaacgct attaacgccc caatacaggg ttctgcagcc gatataatca
    16081 agctggccat gattggcatt cactccgagt ttaaacggtt gaagctgcag tcgaaaatga
    16141 ttatacaggt tcacgacgaa ctggtgtttg acgtttacca accggagctg gagcaggtta
    16201 aggaaattgc caggtcgcaa atggagggtg ctgccgcctt acaaattccc ctggtggtgg
    16261 atattggggt aggcaaaaat tggctggagg cccactagct gcatttctcg aaacggagaa
    16321 aaatgtctaa tcggccgaat ctgcccaggg tgaggggttt atactgccat ctgccacaaa
    16381 atcaccataa gttgacctga tcagttcaag taagccttct atcacctctg gctcggtaga
    16441 ggtaagcagc tttagcctga tttctttaaa attgggaagt cccttgaagt aggctgaaaa
    16501 atgacgccgc atttcgagga ttccggtttt atgacctttt acctcgagcg atttttgaaa
    16561 atgcagcttg gcaatttcca ctttttggct ggctgtaagg ggctccatga gttggccggt
    16621 agaaaggtag tgcttaatct ccctgaatat ccatggcctt ccaaatgatg ccctgccaac
    16681 catgatgcca tctacaccgt gtttttcgaa catctcaccg gctatcaccg gaccgtcaat
    16741 atcgccgtta cctatcaccg gaatggttat tcgggggttg gctttcaccg caccaataag
    16801 agtccaatcg gctttgcctt tgtagagctg agctcgggtg cgcccatgaa tggttatagc
    16861 cttgattcca acatcctgca gccgctcggc aatctcaaca atgttttttg attcgtcgtc
    16921 ccaccctaga cgggtcttca cggtaactgg aatgctagtg gcctccacaa ttcgtttggt
    16981 catctcaacc atggcattga tattcctaag cattccggct ccagcacccc gattggctat
    17041 ttttttaacc ggacagccaa agttaatgtc gataatatcg ggacgacagg ctgtagcaat
    17101 ccttgcggcc tctaccatac tttcaataat atgaccgtaa agctggatgc ctattggtct
    17161 ttcgtagtcg taaatattca actttgccac gctgctgccg gcgtcacgta taagcccatc
    17221 ggcattaata aactcggtgt acatcacatc agcgccgaac tgcttgcata cataccgaaa
    17281 cgacggatcg gtaacatcct ccattggtgc taaaaaaaga ggcttttcgc ctagctctac
    17341 ttcgccaata cgcatttgtc atgcagttga gctgcaaaag taaaaaatgt ttttccctct
    17401 gcataaccaa cgagccgaaa gcgctggtct gcaaatgagt aaaaatagat tttatcggct
    17461 tctgattatc tggcattttg acaagtaaat ccacagttta ccgaaaactt ctacctttgc
    17521 aactgcactt taaccagttg ggttttgctt gagttttttt tcgctatggt atccattttt
    17581 tccctgcact aattctaaca accatcgtat gagagccgga attctatcga atctttctga
    17641 tgctaaaaaa attgttgcgc tgattatcct tgttctatta gggtcgtttc tttttaccct
    17701 actaggctac ctggttataa tggcagtgta tggaaccgat tcgcttggtt ggctaatgaa
    17761 cgtaacaata tcgaacgata caattggtgc gttaaagttt ttgcaaattg tacaaacaat
    17821 aggcatgttc gttgtgcccg gattcacagc agcatggcta ttttccagtc atccatttgg
    17881 ctacctgggt tttaaaccat ccaccgttca gaattttata actgtagccg taatgatggt
    17941 tatctcaata ccggccatca acctgctggc ctccattaac gagctgatac ccctatcggg
    18001 ctggatggta gaaatggaga gaaacgccga gattctaact gaggcatttc tgaaaaccga
    18061 cagcgttgga acgtttctgc taaacctgct gatgattgcc attcttcctg ccgttgggga
    18121 ggagataatt tttcgaggaa ttttgcaaaa acacctggta tcagctaccc gcagcaggtg
    18181 gctcgggata ctgataacag cagcgctatt cagcgcattc cacctccaat ttagagggtt
    18241 cattccccgt tttgcgctgg gtgtcatttt tggctacctg tatgagtgga gtcgcagcct
    18301 gtggctcccc atgctggccc atttcaccaa caatgcaatt gcggtaattg ccttcctgct
    18361 tatgggttcg gaaatcagcg taaacgaaat agaagaggtt ggtagccttc aggacatttg
    18421 ggctattgga attgttagct tcctggcagt agcgttgctg cttcgattta tatggaaaac
    18481 caacacccgg cagtcgccgg taagtcaaaa catggtaaaa agctaactgc taaaaacatg
    18541 cagcttcccg gtagcgtcca tgtggatcag tgtgaaggtg aatgcgcgtt agttgaatag
    18601 ctttagcagc gtcctgtctt ctaaccggaa atcctttatc tcgttcttgc ggtaaacgta
    18661 aactacccca tactccgaag ctttcaaccg gcgttcttcg tcgggtaaat ccttaaagta
    18721 gtcttccacc tcgttccaaa tacttaggat gatgctatcg gcagtaaccc taaggttgtt
    18781 aacatcgtta agcagcctgc gcgtgttctg ctgcaatatt ttatggtggt tgtaagcctc
    18841 taggaactgc tcgtagcgaa ctcgcacaac cgcaatggtt gggttggtga ttggtgtctg
    18901 tcccttttgc aacctctgca tctcgccgtc aataatcttt ttaccccatt cgatcagctc
    18961 cttctcggtt gtcaggctcg gcattctgtt ttcgtccaaa tccatcccgt aaatctccct
    19021 caccgaaaca ggaagctcgc ctcgggcaat tgccatgttt aaaacctgta tgaagtgcga
    19081 aaggtaaagc tttgcctttt tcaaaatttg agagtactcc ttgccgctct ccacctgctg
    19141 gttgtaagcc accttgtaat gagccagcgc attctggaaa gagttaatga atagctcaac
    19201 cttgctgaag gttgactgcg aaaaggccag ctgcgatgga ggatttaatt ttcccttgct
    19261 gtatgcagcc tttaaagcgc gtatccttgc gctatctgtg ttgggtaacc ggcggtaggg
    19321 cataatttaa tcggttgttg ggtttttcgc tctgagaatt aactacctac aatttgcgaa
    19381 tataaaaaat catgattaca aaagccatct attcgcatta aataattttt aaattgcatg
    19441 gcaaatattt tgcttagtag atattttttt gagaaatgaa gaaaagaaag gcgatttcat
    19501 agctcgcccc gcataaaatt cttctacttt tgtaaagaga atttaaaatg attataccat
    19561 gtacggtaaa attcagaaag atttacagaa gcagctcgag gctattgtaa acgacggcct
    19621 ctacaaaacc gagcgtgcaa tctctacacc acagggcgct gaaattaagg ctaacgggaa
    19681 aaaagttttg aacttttgtg ccaacaacta ccttgggctt tcatcgcatc ctgctgtgct
    19741 ggaagccgga aagaaagcca tagacgaatg gggttttggc atgtcgtcgg ttcggtttat
    19801 atgcggcacc cttgagctgc acaagcagct cgaagccaag cttaccgact tcctcggcac
    19861 cgaggatacc attctttact cttcggcttt cgatgccaac ggaggggttt ttgaacccat
    19921 tttgggggaa gaggatgcca ttatctccga cgagctcaac cacgcctcca ttattgacgg
    19981 ggtaaggctg tgcaaggctc agcgcttccg gtataagaac aacgacatgg acgatctgga
    20041 gcgcgtgctg aaggaggcct ccggttgccg ctataaaatg atcgtaaccg atggcgtttt
    20101 ttccatggac ggcataattg cccaggtaga taaaatttgc gaccttgccg ataagcacga
    20161 tgcgctggtt atggttgacg actcccatgc cacaggattt gttggcaaaa ccggaagggg
    20221 aacttccgag tactgcaacg taatgggcag ggttgacgtc atctctacca cttttggtaa
    20281 ggctcttgga ggagcttcgg gcggttgcat ttcgggacgg aaggaaattg ttgacatgct
    20341 tcgccagcgc tccagaccct accttttctc caactcgcta atgccggcca ttgccggatc
    20401 aacactcatg gttcttaaca tgctcagcaa aagcaccgag ctgcgcgata agctttggga
    20461 gaacaccgcc cttttccgaa aaggtatgac cgatgccggt tttagaatcg tacctggtgt
    20521 tcaccccatt acccccatcc tgcttggaca cctacccaac gatgccaagt tgtcgcagga
    20581 ttttgccgat atgctttttg acgagggcat ttacgttacg gggttctttt atccggttgt
    20641 tccaaaggga aaggcccgca tacgtgttca gcttagtgct gcccacagta gggaaaatgt
    20701 cctattcgcc attgataagt ttactaaaat tggaaaaaaa ctcggtgtta tatagccctg
    20761 ttcacaatgc caatcaaaat aagaacggac ctgctaaact aacagcaggt cttttttatt
    20821 acagcacagc gtaggtatga ttcaacgtga aatgggtatg actcgttatg ttgtgctgcc
    20881 taaccagaat gctgacttaa caagacgaac ctagggtatg aattccacaa aaatcaaccc
    20941 taattatttt gtggttgcaa tttctcggct tctgtggcct ttttccctcc caaacttttt
    21001 tcttacccag cgcggaaaaa ctattgcacc aatgaaaagg taggggtagt agcttattaa
    21061 ccgccagagc atggccattc caacagcaat ggaagtgagc gattctgctc ctacgggaat
    21121 aaaatctgac aggtagcggg tgaatacaaa ttcggcaaaa ccgcttcccc caggcgtagg
    21181 gcttaccagc atgataatcc acataaccag ctgtcgggta aaaattagca agtggtggtt
    21241 tacatcaaaa aatgcaagta gaagtgcgtt tatcaccaaa aacctcgacg accatgaaag
    21301 gaatgaagaa gcaacggctt tagcccaaaa agagaatggc tttttcttga actcctccga
    21361 gctcgttatt atatcgcttc cggccttgtt tgcaccctgt ttccacctgc gcagcaacgg
    21421 cagcttaaaa atcatcatga taagccattt cagcccccgt gggtttataa aaaggccata
    21481 cgataagcct aaaacccaaa ccaactttaa cgaatagcca actatgctga aaattataag
    21541 gtctttcgcc ataccaccag cgggagaaga agcaagggta aaaagttcgt gagggccggt
    21601 taaaagaaca agaatcggga acattactac aaaatatagc tcgtccagaa aggaggttat
    21661 cataacaact gccgagctgc gacccaccgt aagaccttcc ttattgacgt agacaacagc
    21721 tacgctggtt ccgccaacag cagaaggcgt aatggccgag gtaaactccc atagcattat
    21781 tacccggaag gctttccaga acttgaactt cccttcggaa agggtcataa gcctgaaggt
    21841 atagcccata tcccttcctg ccatcaaaag tgcggctaca agtatccaaa agaatgaacg
    21901 ccaggtaaag cttatctgca aaaaggcttg agggttaaac tccctgaaaa gcattaaaaa
    21961 aaccaccccc agaccaatta ttacggggat gatagcagtt ccaaccttta tgcgtcgggc
    22021 tggattttca gaatagtggc gttttctttt cggcatgagc agtctggagg taaaggctac
    22081 ttggtgctaa cgctattcac aatcataaca aacttttaac taccactcca tttcaataaa
    22141 ggtgttgttg cttcctgcta aaactctgta cccatctggc gtgggtagtc aattgagtag
    22201 tgaagccccc tgctttcgtg catattttgg gcatccttaa taattaggta gccaatttga
    22261 atgaggttgc gcagctcgca taactccctg gtgagtatgg attttttgta aagttcctca
    22321 gtttcttcaa atatgatgtc taaacggtta agagctctcg aaagccgaag gttagaacga
    22381 acaataccca catagttggt cataatttgc tgcatttcct tgtagctttg agtgataagc
    22441 accatttcct cgttgtgggt ggttccctcg taatcccagt cggggattct ggtgctgttg
    22501 ttaatactct taaaagttgc cagtgaatgc cgggcagccc tttcggcata aacaatagct
    22561 tccatcagcg agttggaagc aagtcggtta gccccatgca gcccggttga cgagcattcg
    22621 ccaatggcat aaagcctttt aatggctgtt tgaccattca tgtcaacctt aattccaccg
    22681 caaaggtagt gggctgcagg cacaacaggt atcatatcct tggtaacatc aataccaacc
    22741 gataggcatt tctggtaaat gttggggaag tggttgatgg tgtcatctgg atccttaaag
    22801 gtaacgtcga ggtaaacgaa gtcatcgcca cgctgcttca tttctgaatc tatggcccgg
    22861 gcagtaatat cgcggggagc aagagaaccg cgagggtcgt acttctgcat gaactcctta
    22921 ccgtcggatg ttttgagcac tgcaccgtag cctcgcatag cttccgatat gagaaaagac
    22981 ggacgttccc ccgggtggta gagggatgtg gggtgaaact gcacgaaagc catattttca
    23041 acggcgccct ttgccctgta aaccatggca attccatcgc cggttgcagt tctgggatta
    23101 gtggttgtgt ggtagatatt gccagaaccg ccagttgcca ccaccactga cctgcctaaa
    23161 aatgtatgaa ccgcttgagt tttttgatcc agcacatagg ctccaaaaca ggtaatgtcg
    23221 ggatcgcccc ggtgaataac ccgccccaaa tggtgctggg ttatgagttc aacggcaaag
    23281 tgattttcaa aaacctgtat gttgggatgc tgacgaactc cagcactcag cgccctctga
    23341 atttcagcgc cggttatatc cttgtgatga agtattcggt gctccgaatg acctccctcg
    23401 cgggctaaat catactgccc gttaggcttt ctgtcaaatt ttacgcccca ctggattagc
    23461 tggtttatct gggcgggggc ctctgaggca accatgcgaa ccacctcctc gttgcagtat
    23521 ccatcgccgc atatcaaggt atcgtttaca tgcttctcaa aagtgtcggg agggtaggta
    23581 actgctgcaa ttccaccttg agccatggag gtgttggtat cgtctatccc cgctttagtt
    23641 accagaatta ccttgccata atctgcaact tttagggcat agcttaagcc cccaattcct
    23701 gagccaataa ctaaaaaatc acactgaaag gttgacatgg tagttaaatt tcatctaacc
    23761 tgtagtactg aatgattgat aaaaaaacgg gtaaaaatac aaataaaccg actagtgtgt
    23821 catagttagt taaattcgaa tggcgcttcg cttacgctcg gagaaggttg aaacttcctt
    23881 atatcccaca taccgtagca gggcaatggc atcggtaaag tactgcccta tgttttgctc
    23941 cacatgggca tcagagccca gcgtaaccgg caccttgtag tgatgcagaa tttccagtag
    24001 ccgaagggag gggtaaaact cggcacaggg cttgtttttc ccgctggtgt tcagctcaac
    24061 ggttaccccg taatccgaaa agactcttgc cgtttcctca aagattcggt caaggctgat
    24121 ggtggggtaa taggcaaatt tcttggccag gtcgcagtgg ccaataatat cgaaaagtcc
    24181 cgattttgcc gactggcaaa ccagcttgaa gtaggtttta taaaaagcgt ttatgctgat
    24241 ctgatcgtag ggcagaatat cggtatcgaa attccaatcg tctataaaat gaacagaacc
    24301 aatgcagtag tcaagcggaa taaagctgag cagctcccga gtttcggttt cccgcccggg
    24361 tatgaaatcg acctctgcac cctgtttaac cctaaccact ccttcttttt gcttaagcat
    24421 ttgaaccgct tcaaccatag caggaatatt tctgacatcc attgcccagc ttacgggttt
    24481 tacgcagatg tgatcggaaa atccaatttc agaaacatct ctaatctggg ccgaagcgaa
    24541 catttcctcg tgagtgtcgc gcccatcgga taggttggtg tgagtgtggt aatcgggata
    24601 catgatataa atttcaacaa ttatgggtaa aaaatgttgc caactaccaa aagtatgcaa
    24661 ttttttacca aaatttttcc atgcaaaatc cactactggt aaaacgctta actttacagg
    24721 ctgcaactgc tctaaagcca gctgtgattc ctttgcaaat atgttaaagt ttgaacgatt
    24781 agatggaaag cccatttagt tagcataacc ttaattatta agtagttcgc taccgcataa
    24841 ccattaaatt atcaaggtaa accatgagaa caaatctact agggataatc gctatttcag
    24901 gcgtaatatc cttgatggtg tcgtgccaac cccaggctga aaagcaggag tacaaccagg
    24961 atcacatgct gtactccaca atttggtacc agcattcacc cgaaatgcag gcgctatact
    25021 accaggcttt caacatagcc ggcgaacgtg ttagacagca atcgtcgcac aagacctcaa
    25081 agccaaaagc agtggtggtt gatattgatg aaacaatgct ggacaacagc ccatttcagg
    25141 cacaggaaat attagacaat agggaatact ccactgaatt ctggaaggaa tggacatccc
    25201 ttgcacgggc aaaagccctt ccgggtgccg ttgagttcag caaactttgc gattcgctca
    25261 aagtgaccct tttctacata accaaccgct ctgctgacga ggcagcatca accctaaaaa
    25321 atctagactc gttaggtttt gcatttgcca aacccgagta ccttctgcca aaggagaacg
    25381 aaagcagtaa gaagcagcgc cgtgaaagga ttgcggaaca gtacgatatt atactgctgg
    25441 ttggcgacaa cctcaacgat ttctccgaaa tatttgagaa tcgcggcgac gattgggggt
    25501 ctgggttggt aggcaagaat aaaagtgatt ttggcagccg gttcatcatc cttccaaacc
    25561 caatgtatgg cgattgggaa aaaaacattt accatacaag ggggctttct cctcagcaaa
    25621 aggacagctt gcgtcgctcg cttttaaatg gattcaggta acattcaacc tttgtttcta
    25681 cccaactagc tagtaggata tttgcatcat tctgtgtggc tgctggagtg ctgtatataa
    25741 aattagccat gctgcaaatt ctttagcatg aaaatgttgc tgttagtgtg taatgcgact
    25801 aacaacagta tagtctttaa gtttgtgtct ccacaatccg gaggtaataa aaaaagcctt
    25861 tatgagctta aaggcttgtt gttaggttgg cggccttacc aggactcgaa cctggattta
    25921 aagtttagga aacttccgtt ctatcccttg aactataagg ccataaccag ctgcaaagat
    25981 aggaatattt ttatatgtta accaataatc caaggggttg catctttacg cctgttgata
    26041 actcatgcaa actgttaatt actatcgcct tccccatgaa taaaaatgct gatagcaact
    26101 taatttcgtc gaaattttta aaaacaccgg cattaatgat tttttagcta aaaaaattaa
    26161 gaagcctgaa caaggtattg aataaataac aacatttgcg acgctaaatt ttaaagtcat
    26221 gagcaaagaa ctaatgcatg aaacgcttaa cacgctaggc ttagaagaag atgatgaacc
    26281 tccgggttcg gggcagctaa gcccaaatta cggtttaatt accgcaactt cccatcaaca
    26341 caaatcaccc tcctgcaatt ggccatttcc aatcagcaaa cgttcgctgg aattcttgat
    26401 aaagtacttc cccaacgcca cggtggaaga ctggatgagc tggaaatggc agctgaaaaa
    26461 cagcttcaca acttccgaaa aactcaaatg cttccttgat ctttccaggg aggagtgcga
    26521 ggcaattgac caccccaaac attcgctgcc attacgaata acaccctact acgccagcct
    26581 gctgaatggg aaaaactccc aggacccgct acgtcggacc atgataccca ccgttaacga
    26641 gcttaccacc tcggcaggtg agtcgtgcga cccattaaac gagggacatt ccagccccgt
    26701 tccaaacctg gtacatcgct acccggacag ggtgctgttt ctttcaaccg gattctgctc
    26761 ggcctactgt cgttactgca cccgttcgca catggtggcc aaggataagt gccatgttgg
    26821 ggtcaacgcc tggctaccag caattaacta tattagggag cacaccgagg ttagggacgt
    26881 cctcatctcg ggtggcgacc cgcttaccat gtccgatgca cacatcgagt acctcctttc
    26941 ccagcttaga agcattccac atgttgaaat gatacggatt ggcaccaagg ttccggtcgt
    27001 tcttccccat agaattactc ctaagctcat aaaaattctc cggaaatttc atcccctcta
    27061 catcagcatc cattttaccc atcccgatga gcttaccccg gaaacagccg aggcatgcag
    27121 gcggcttgcc gatgctggta ttccgcttgg tagccaaact gttcttctta aaggtattaa
    27181 cgacaatgtg gacaccatga agtccctatt tcaaggactg ctaaaaatta gggtccgacc
    27241 ttactacctt taccagtgcg atcccatacc cggctcttcc cactttagaa catctgttga
    27301 aaagggcctt gagataatta aaggacttag gggctggacc accggatacg ccattcccca
    27361 ctttgttatt gacgcacctg gtggcggtgg aaaaattccc cttctgcctg aatactacca
    27421 ggggagaaat gaggagggat acgttgtgct taaaaactac gaggggaaaa ccttttacta
    27481 ccctgattta cattaaaaca atggcaattg tatagttcaa ccacctgtcc ctttgctttc
    27541 acttacttcg gacaggtggt ttttaccata acgctattta ctgcacacct ttttcaaaaa
    27601 aataaacttg atgcgctcaa atggctttcc caagagtttt taatgctcac gctgataaac
    27661 acaaaaatag atgacagttg gcttaacata cgacctgaaa agcgactacc tgaaaaatgg
    27721 ctactcacat gaggaagtgg ccgagtttga ctctgacgaa acaattgaca gcattgagag
    27781 tacactgcag tctcttggct acttcaccga gcgggttgga aacatcatca acctcaccca
    27841 tgcgctggcc aacggcaaac gttgggacat ggtttttaat attgccgaag ggctgcacgg
    27901 aattggaaga gaagcacagg ttcctgccat tctggatgcg tttcaaattc cctacgtgtt
    27961 ttccgacccc ctggtgctgg cacttaccct acacaagggg caggcaaagc gtgtggttcg
    28021 cgactgcggc gtgcctacag ccgatttcgc cgttattaca agcctaagcg aggttgagaa
    28081 aatagctctg cccttccccc tgtttgtaaa gccagctgca gagggaacag gaaaagggat
    28141 taatggccag tcgaagataa actcaaaaga agtgctctat gcaaccacag cacggttgct
    28201 cgaagaatat cgtcaaccta ttcttgtcga gacctacctt cccgggcgtg aattcaccgt
    28261 tgggatagtt ggtacaggcg ataaggcacg ctgcctaggg gttatggaga tagtgatttc
    28321 caacagagca aaagatggta cctactccct acacaccaag gagaattgga agggcattgt
    28381 ggactactac atggcaaccg gcgaaataga atcgctttgt gccgaaactg ctctaatggc
    28441 atggaattgc ctgggttgtc gcgatggtgg aagggttgac ttaaaaatgg atgcttcggg
    28501 caaacccaac ttcattgagg ttaatcctct tgcaggcctg aatccattcc actctgattt
    28561 ccccatgctc gctcaaaaaa atggcattga ttttaccatg cttattcagc tgataatgga
    28621 ttcggcaatg gaacggttta atcatacggc atgaacaggc aaacaataca tgcggtaatc
    28681 ctatacaacg agctttcgga gagcgccggt cccgacgaaa gggatgttct ggaccaggtt
    28741 gcccttgttg ccgagatgct caacaagctg ggtattgaga gccagcgcgc ggaattttcc
    28801 cttaaccttc aaaaggttcg tgatgagctc agcaggctaa agcccgactt tgttttcaac
    28861 ctggttgaat cggtgaacaa caggggaaac ctgattttcc ttgccccttc cctgcttaac
    28921 tctatgggaa ttccattcac cggaggctcg ctcgaaacca tattcctcac atcatccaaa
    28981 acgctaacca agcagctgat gcaggatgca ggcatcccaa cgcccttttg gtaccaggga
    29041 aacggttcat ttattcccga tcgggataaa cgttacattg taaaaccaat ctgggaggat
    29101 ggatcgctgg gcctcgatga ggattcaatt tttgagggaa gcaacccgaa actagccgaa
    29161 attttaaata gcattgacta caacacccat tttatagagg agtttgtgga cggccgcgag
    29221 tttaacatat ctattttggc cggacaaaac ggtccacaac tattaccgcc agccgaaatc
    29281 atatttcgta actatgctcc cgacaagccg catgttgttg gctactccgc caaatggatt
    29341 gaggatagct ttgagtatat caacacaaac agaacttttg acctgtctcc aaatgaccaa
    29401 ccgttgctca gcaccatggg tgatatttgc cttcactgct ggaaaacctt tagggtgaat
    29461 ggctacgcca gggtagattt tcgggttgat aacgcaggaa acccgtgggt gctggaaatc
    29521 aacgtcaacc cttgtatttc gagagatagc ggattctatg cggcttgtat gcaggcagga
    29581 attccgtttg atgaagcggt aaggagcatt atcgccgata tcccctttca aatttaacat
    29641 aacgttcagc cttatggaca atgtaacttt tcgctccacc ccaacgcccg ccgacaaagc
    29701 cggtatcaga gaaatactat cctcatctgg attttttcac gattttgaaa ttgatgtagc
    29761 catagagctg gtggaggagg ctttggcaaa gggaacggaa agcggttact actttgtttt
    29821 tgccgaccac atgggcacta cgatatccta cgcctgtttt ggtccaattc cttgcacacg
    29881 ccacagcttc gatctgtact ggattggaac ccaccaggat tacaggggga aaggccttgg
    29941 caaacaggta ctgcaaaggg tagaaaatga gattaaacag ctgaatgggc gaaaaatata
    30001 cattgaaacc tcatcaaaac caatgtacga gcccacccgg caattttacc ttagcaatgc
    30061 ctacgagaac gaggcaatac ttaaaaactt ttatgacgaa ggcgacgata aggttgtata
    30121 ttcaaaaagc ctttaaacat aaaccagcat attctaagcc ctttttgact ttttatcttt
    30181 gcaaagcaaa ccagtcagga aatgaatcag agagatgcat ctactaaaaa tcaaccttca
    30241 ttttttctca aacccgacat ggagaaagga gttgccatgt catcaattgt ccttcatgtc
    30301 atcatttaaa atagaagcaa cttgttttaa tactgccaag taccgttcga aattaagcac
    30361 cgtttttttt gcaacacaac aaactccctg aatatgtaag cgttttagga aatcattgca
    30421 aaaaaattaa tcagataagg ttaattttta taatttattt tgtcttttta acaatacatt
    30481 tttaatttta tcacccaaaa cgtatcaatt caaaacaacc atgcaggaaa aatttagggt
    30541 agatgtaatc gatcagaaga tactttcgta cctggtaaaa aacgccagga taccatttct
    30601 tgagattgca agggagtgtg ggatatcggg agcagccata catcagcgag taaaaaagat
    30661 ggaagatgcc ggaataattg ccggatcgcg aatgattgtt aagcccaaag ccttagggtt
    30721 tgacgtatgt gcatttgtag gcgtgcagct tgtttccacc aacctgtaca gcggcgtcat
    30781 tgaagccctg aagctaatgc ccgaggtggt agaatgccat tttataacag gagagtatgc
    30841 tttgctgctc aaaatctact gtagggataa cgagcacctg atggacgtgc tggttaacac
    30901 cattcagaat atacccggca tatcccgaac cgaaactttt atctcgctcg accaggcaat
    30961 tgaaagaccc ttctatgtta aggataagag cgttgtgcac aacgaaaagt aaaagaaaaa
    31021 taaaaaaggc tgcccttccg gcagccttac ttgattatct atagccctga gtattaccta
    31081 ctacagctct tcctcctctt cgtcatagtc ttcaaatttt tcgtccaaca ctgtcccctt
    31141 gaaatctacc aggtcatcaa gctcatcaat ttcactgaag attgaacgct tagcattttt
    31201 ttcctttttc acgggtttta gctttttaac cattttcacc tcatcggaat cgtaggagtc
    31261 attctttacg ggtttccgaa aatctttctt tgacttcatc acatgagatc tgttaaaagt
    31321 taattctcat ttagttttaa tttgctgatt aatagattat agttaaagtg attaaacgca
    31381 agtttaggta attttcttaa tagaagctta gccttttcga caaaaattag ttttttttag
    31441 acacgttcat tggtggcggt aaaaaaatcg ttttttatca cttaaacgaa attatgaaat
    31501 tttagtttta ccagtacgcc agatgatttt accttatgcc ttaggttgga caataatcga
    31561 acaaatatgc cctacaaaaa gggagaattt gaagtggggt ttattgcagg tcttcctcac
    31621 tcgagcaagt cacagaacgc ggtattttta aaatgctacc tgaacgtata aaaccaaaag
    31681 tagtaatttt gctaaaaatc ccaacagatg gataaaggtg aagccaataa gcgttggtat
    31741 gccctgtaca ctaaaccgcg gaatgaaaaa aaaatatata gccggttggt ggaaaaaggc
    31801 gtggaagcct ttctgccctt acagaaaagg ctaaagcagt ggagcgatag gaaaaagttg
    31861 gtggaggagc ctttgttccg gtcatacatt tttgttcgga ttgatgcccg ggagtactac
    31921 gaggtgttga acactccagg aattgttagg tacatcacct ttgagggaaa ggccgttccc
    31981 attcccgaca ggcagattga gcaggtaaaa cagctgctgg ttcaggattt ggaaattgaa
    32041 ccggttgaag agcaaattcc accaggagcc ttggttgaag ttagatttgg taacctgatg
    32101 gggattaccg gtgagctggt tgaacatgcc ggaaaaaaga aggttattat ccgtatcgat
    32161 catgtaagcc actccctgct ggtaaccctt cctaaggatt atgtagcacg agccgtgtag
    32221 gaactatttc ccccgtattg cttttctcaa cagggctggt agtgctattc gaatagaaaa
    32281 tccatgtcat cacccgggtt aacctcaaat gggggaatac cttttttgaa tattctggca
    32341 gattttggtc cttttagggt cagagcaaca ttctccagca gaatcatcga accctcccgc
    32401 aaaccaacca cgtaaacgta tggatccatt tcgaggaact ccataatgcg ctgctcccgg
    32461 gtttcgccag catgtcctgc ggggtttgca tccaggtagt gggggttaat ctgaaagcga
    32521 atcaggctaa aggcaccaaa gttatcgggt tcggtgatgg gcatatcgtt ggttgtgcaa
    32581 atggtggggc aggccacgtt ggctccagca ctccagccta tgtagggggt gccatccaaa
    32641 accttattcc gaacagtttc aactatatca tgctgctgta tagtcttaag cagcttaaag
    32701 gtgttaccac caccaacaac aatggcttca gcctcctgaa ccgccgtttt aacatttcga
    32761 tagtggtgaa tggagtcaac ctcaacgcca aactcgctga agcgttcttg taccttacgt
    32821 tcaaactcat cgtagctaaa agttacggct gcccacggaa tgaaaagcac ccttttagtg
    32881 ccattcagaa accttttaag ctcttccttt ggatgttcca gaaacgcttc ccctgcattt
    32941 gttgagttgc ttattagcag cattttaacg gtcttctttg actggctatc catagtaatt
    33001 agttgttata agaattaatt ctcaaatttt taaattctaa gggcaaatat ataggtaaaa
    33061 ttttccagct caaacgtttt tagcaagttt tgcattgcct ggggttgcag ggaaaattcc
    33121 atagacgcaa cactgcctat tattttttcc atttctcgct gctctctatc tggcaagggt
    33181 aaacctattg tagtaaagta aaggtaaaga gtatcaccct tcacaagttc tggacattgg
    33241 aacgctttag ggaatatcag cttattgcta tgccatacgg caaaaccctc ctctgtaagg
    33301 ttcaaaacaa agtctccatt aatagtaaaa ggcaaaatac agtattgtga agggtcaaga
    33361 tcactagtaa gcgggtcagc tgcaaaattc tccctcatct ctttcataaa atttacccac
    33421 gatatcatga gccgatgggc attgctttta acccagcagc acaggcatag agtaatgagg
    33481 ttggggctaa ctctttggct atcacttacc tgcatgaaag caagggtaga attatttacc
    33541 ttaaagctga gcaacttgca ggtaccctgg gtgctgttag ctggttgcaa tttccagcta
    33601 tcggaaaaaa atcctcccac tgtgccatca tcagccacct ttaaagtgtt ttgaagaaat
    33661 gcatctagcc gttttactgt gctttcttta aatttttgtt cctcggcaag agctgcgttt
    33721 ttcttcttgt tttccctgta aagcaaaaca agcaaacccc ccatcatggc taataagcca
    33781 ctcaccgtcc aggcaacctg ccgctgtagc tttaacttgt gctgaaccag agaaacctgc
    33841 gctgcctgat tttccatttc aagttgtgcc tgcagggttt cgattttctc aatggtttca
    33901 gcatttagca tggaatcctt taaaacctga tagtttttta agtggtaata ggctttagga
    33961 aagcttccta ttacctcata tgcgtttgct agcgtttggt gcacatctcg tgcaacatct
    34021 ttttgacctg ttccaacagc catatccagc gactggttaa ggtaggctac cgcaacatcg
    34081 gggcggtttt gcttcaggta aacacttccg atgtttttaa gggtaaggca tttacgcgta
    34141 tcgcttttca gctcattaaa gatttgaaga gcgttaaaat ggtaaagaag ggcgttacgg
    34201 taatctccct tactatacaa gagcgatccg atattatcct gtatgatacc aacacctatt
    34261 tttaccccca acgaatcata gattttgaat gctttcatgt agtaatcaag cgctctatcg
    34321 aggtggttga gctccatgta agcgctggca atgttgttgc atgcccccgc aacggctcta
    34381 tagtttccta aaccttggtt aatctccagg tagctggaaa agtaatcaac tgccttagtg
    34441 tagttttcgc ggtagtagta tatttggcca atgctgttgg aagccatagc catcccttcg
    34501 gcgttggcta acctttcgta aagcttatag gatttcaaat agaactccag cgccgaatcg
    34561 taccttcccc agctgcggta aatcaagccc atatcgttgt aaactcctgc cagatcggca
    34621 ttgtcattta gagagacgta cagctcagcg gcattttgaa agttggcaag cgcacttttg
    34681 ggctcgccca tttttaggta ggtaaatcct atttgatgta ggatgtcggc agtatattta
    34741 ctgctttcaa gtttttttgc cacctccagc gcttctaacc agtactccaa gctcttgctg
    34801 tagctgctat cggaatagga aattgcaatg ctgctaagga ttttatactt tccctcgctg
    34861 gattttacgg ttgaaagttc atggagaagg ctatctaaag acaatggctg tccccatagc
    34921 cttggtgtaa ccaaggagag cgatccggca agcaatatgg ctgctaaact ccctttcaag
    34981 gcatcccttc acaagataaa gacaggttaa ctatttaata agcggcttgg cttgaaagtt
    35041 actagtttca aaggaaaaaa caaatttaaa gtatagcgat aaacagcacc atcctaaaac
    35101 aaatacggta agcacccttg gttgggcagc acaattcaaa gaaagtcctt taaattcagc
    35161 ctccaactgc caaagtttaa aaattatgca ctaacggcta cacataaaag ccacagtaca
    35221 attcaatcct tatagcatat tacaaataaa aagattgagc agtaaccatg tggcataccc
    35281 cccgaaaggt gcctatatta tccttttgaa atgcttgtag agttcgaacc agataattgt
    35341 tgataatcct gccaacgtgc aaatccccaa ctcaagccat ccaattttat cgaacaggaa
    35401 gaggttttgc agtgcaggaa tgttaacaat cagcaataaa aacagaacag caccacctac
    35461 aatccactta accgttttgt tgggagtcat aataatcttg aaaatagaat ccgaccatga
    35521 gcggtttgat aggataactg cgatgttgga aacaataagg gtgataaagg taaatgctct
    35581 tacggctttt tcggcatacc ccattcgaaa gcctatgaag taaaccagca agctaactgc
    35641 aagaattccc aacccttggg tgcagctcat caggattttc ccgacaccaa agaagggctg
    35701 attgatgtta cgtggaggac gtgtcatgac attcttttcc tccttttcgg cttcaaatat
    35761 tatggagcag gccgggtcaa tgattagctc caggaatacg atgtgcacag gccaaagaat
    35821 aagtggcagg ttggcaaaga aaaccggaat aagcgatagc cccgcaatgg gcacatggat
    35881 ggcaaaaata tacgccaggg ctttttgaag gttgtcagaa atttttcggc ccatccgcac
    35941 cgccttaaca atcgaggcaa aattatcgtc catcagcacc agcgaggagg cttcccgggc
    36001 aacatctgtt cctttctccc ccatggcaat tccgatatgg gcggctttga gcgcaggggc
    36061 atcgttgacc ccgtcgccgg tcatggcaac aatttcatca ttccgcttaa gggcatttac
    36121 tagcttaagc ttctgttctg gaacaacacg ggcaaagatg ttaacctgtt taatccgctc
    36181 accaagctct tcctccgtca tggtttccag atcctgaccg gtgatgtaaa gttcagggtt
    36241 tttgatgcct atttccctac caatattggt agccgtaaca ggatagtcgc ccgttatcat
    36301 aatcacccgg attccggctc tgtagcactc ggcaaccgcc tcgggaacat tctccctaat
    36361 tgggtcggag agcccaatta gccccataaa ctcaaagttg aagtcatgct gaattgtggg
    36421 caggttttct gttgaaagtt cagcacgagc aactccaagt actcgcagcc ctctagaggc
    36481 catttcggcc accgcctttt cgtgcttgcc aacttcagca gcagggagat ggcataagtc
    36541 aaatatggcc tccggagcgc cttttgccgc aataaccctt ctgcctgttc cggtatttga
    36601 aaacacccgc gacatggcca acagctcctt tgatagcggg tactccttct ccatcaccca
    36661 atccttgtgg atgtgctcag aattctgcaa gtacttatcg ccaatgctga taattgccct
    36721 ttccatcggg tcataggggt tcacctggct cgagagtatg gcatactcaa tcacctgatg
    36781 aaagggttcg gtaaatgtgt cgcccgatgt tacagcaata tggttagtgc cattgtagag
    36841 ctgggtaacc gtcatccggt tttgggtaag agtccctgtt ttatcggtgc agagaactgt
    36901 taccgagcca agtgtttcga tggcagcagg ttgacgtgtc aatacgttgt tcctggatat
    36961 gcgccaggca ccaagcgcca taaaaacggc taaaacaacc ggaaattcct cgggaagcat
    37021 ggccatagcc agggttattc cggccaaaaa cccgttgaga aggtcgccac gggtaatggt
    37081 gtatataatc accaccagca agcacaggat gacaccgatt actgccagcc ttttcaccag
    37141 aactgccatc tccctcttca acctggtggg ctcttctttc actgattcca acgccttccc
    37201 aattttccct atctcggtat tgaccccggt ggcggttacc cgaaacactc cattgccgtt
    37261 caccaccatg gagcccgaaa aaaccattgg aatatcgtcc cctcccggaa taaaagagcc
    37321 atctccttcg agccattccc tttttctcac cggaacagac tctccggtta acaacgactc
    37381 atctgctgaa agattaatgg agctgagaat aaccccatcg gccggaaccc tatcgccctc
    37441 ctgcaaaaca acgtaatcac cggttacaac ttcccgtccg gcaatcctta ctgtttcgcc
    37501 atctcttatc accaacgcac gtggactagc caggtttttt aacgcatcca gcgccctttc
    37561 ggtttttttc tcctggtaga attcaattcc catgataaca aacacaaagc tgaggagcat
    37621 aaggccttca ttaacatctc ctaaaataag atacaaagtg ccacaggcaa ccagaaggat
    37681 gaacattggc tcctttacca ccccaagggc tatctgaaaa acattcttgg gttttgagga
    37741 gggcagctcg ttgtaccctt cttctataag tcttctggcg gcttccgctt ttgaaaggcc
    37801 ttgcaggctt tcaggatgaa tagttgtgct cattccttgt caacttaagt caacgtgtat
    37861 ataaaaagtc catgcttatc cgtgttgttc gcttgaacaa cgcttaactt gttagattaa
    37921 tctaactaat cgggtaaaag tgtcaagcat atcaattaat atttcatatc aaattctggc
    37981 aataaaacaa aagagtcctg ttttcaaaac atggccgtta ctctaccagc acttttgaaa
    38041 gtaagcgttc aagcagggtt aactcctccg ccgataagcg gtttttaacc atttcggcaa
    38101 tttttcggtg tgcataatcg tgcatctggt ttaccagctt ccccttctcg gtgaggtgga
    38161 tgtgcgcgct tcgcctatcc tcgtcggact gaattttata aacacatccc ttctcaatta
    38221 acttgtccac cgatacggtt acggtgggct tcgagagctt cagctgggcc gatagttcag
    38281 tgatgttggg gtttcctaac aggttaattg tttccaggta gtgcatctgg gtaatggtca
    38341 ggttgagcgt gttgaactgc tccttggcct tctcctccgc atcgccaatc atgttggaca
    38401 aacccgcaat aactttctcc atgctttcca tacagcaaat atagttagtt tgtacaaact
    38461 aaaattaatg ctaaataaaa tttcggttaa gcgtaggcat aaaatgataa ttttttgagc
    38521 aaaaaaatca gctgaacttt cacattccat tttttacact actctgcaca ccccaaatat
    38581 aatgaaatct accttaagtg cttaatttct ggctgttacg ttttcaaggg ttgccatata
    38641 ttaccataat aacaaaaagt taggataccg tcacagctga atatacgctc gaaagctata
    38701 tttgcaatgt ttaataaccg gatttcaaac cacatgaata acgctattgc aaccccgcag
    38761 gataaccatg aaaggttgct acgaatggtt gaggaaatat ttgccacttc tggcgatccc
    38821 cgccagctga atattacccc cgaggtaatt gaccggctga aacagcttca tccctctacg
    38881 gttttggagc atgttagcga gaatggaacc gtggcctggg ttgtgctgat tcccaccacg
    38941 ctggagctta tgaaccgatt tgtggcaggc gaaatttcgg aaaaggagct ttttgagcta
    39001 acgccattag gcataaagta cgaggcgctt tacctctgct ctgcactggt gctggaggag
    39061 tataggcgac aaggaattgt gaagcaaatg gttactgctg ctgcccaaag cattcgaaag
    39121 gaacatccct taaaagccat ctttgtttgg ccatttacca aagaaggaga gctggcagca
    39181 gaaagcattg ctactacact gaatttgccc ctgtacaaat ttcaacatga taaatgatta
    39241 caacccggag ctagaattag tgtagccatt ggcgggtaac tttaggtcga gccacctacc
    39301 cagcaccttg taaatgctat cctgaagttc gggcggaaaa tctttaatcc ttgacgagtg
    39361 cgaacctccg gggcatacca tttttactgc attggttttt ctacccggat caacagcagt
    39421 agccgaccat gcatcgtttt gcccgtatat gtatagcatg tagctacctg aatcctttac
    39481 ccaacggtca atgtcgctca tggcggaggc gttgaagatc gcccggtgcc ctcttggcat
    39541 ggcaaagcta aaggttatgt tggcggtatc gctaaggtag cggctgaacg gcttcaccct
    39601 gtagccatac atgcctatct cggtcatagc ctgatagaaa aatgggcgaa ccttgtcaac
    39661 ctcattctcc tcaaaaaagg agaaggggtt aacctccagc caatgctcaa aaagttcctt
    39721 tgcggttgaa ctgcttgcag gaattgacga gcaattaaat ccccactgcc agaaagcaaa
    39781 ggagtactca agcacgctaa ggtcgtacgc cttttcgaat cccatagtga atttccaccc
    39841 cttgcttgct gaaagttcct tgaagagtgg cataatttca cttttccttt cgaaaagttg
    39901 aatctgaaaa tcatatattt ttttacgaca ttcgggcgtc ccaacactat caagaaattt
    39961 gtacaccctt tcgtcttcac ttgaaaagtt cagcggagcc acgtagggta tgctaacatt
    40021 tacatcagcg ggatagaagc gccggtggaa aattgtagct tgtccactct tgcttatccc
    40081 ggttgaaatc cacttcccat ggtagaacgg tttaaatgct gatattacca aatgtgcatc
    40141 agctgccgct tgcctaatat tgaggtaact ccagggaatg gagtccaatg gtctgctttg
    40201 agaaaaaaag cgatgctcta tggttatctg atttccatca acaatactgg ttatctcgtt
    40261 agctttcgaa gtgtacattg tgtagccatc aataacaaca accatgggtt tgttaaaacc
    40321 attatgggag tagtaaaccc ttaagggaaa atattggctg tgagggttgg tgtggtcaag
    40381 cggtacccta aaccaaacct cgtaggtttc cgaaaagggg ttgtcagaag gaattgctct
    40441 gaagctatca accagcgcta tggacgacag cacttctgta ggagttatgg gcttggggtt
    40501 gccggaaagt ataaccacca tcaaaaccgg gaagaatata ctcaaaataa ggcctcgtat
    40561 cctcaggggt ctttgttgct gcggcattcg tttcatgcta aaaattttta attaccaatc
    40621 cgaaaatgtt ctttacaacc ctaacgttta cggaattacc tagttgccta agcgcagtag
    40681 ccggcggtcc aataaggttg tattccttgg gaaaagattg aagaagcttg gtttcctcaa
    40741 cagaaattct tcgtctgaac ctgccaataa tctgccggtg gttcatggca accagggttg
    40801 aaaacttatc gggacgctta accctaatcc ccgacggacg aaattgtatg aggcaatcaa
    40861 acaggctgct ataaccatct ccagcctgcc actcgaactt gcgatgagtt tttgaacacc
    40921 attccaggtt atcccatttt gccagccatt tatcaataaa actacgattt cgtctataca
    40981 agtcaatatt ctttctaata aatgtggcct tccatactgg gaattgggat aaatcgcctt
    41041 caaatctaaa aaagtccatc cacacgggaa acccaatcac tttctggtca attcccctgt
    41101 aaaactcatc ccaagcagta agaacttttt cttcgtatgg agaaatacaa tcatcaacgt
    41161 ttggatcaac aatatcgttc accgttaact ggttttcaga cggatgcttc cagatttctt
    41221 cttcaaaatt ccaaataaaa tcctccttcc ctgctgccaa gtccttccta acacagggaa
    41281 tatataatcg tggacgaaga atgggaactc caaatttgtc tggagagaga atcatcgggt
    41341 ttcgtggaaa ataataccct aactcatcga ggcttcgggt aataacgcta taggtattcc
    41401 cgccatcgtg ccccgcaagg ttggccacat tctcgagtaa taaaaatttt ggcctatgct
    41461 tcattacaat ttggcagatg tcaaaaaaaa gcgttccacg tgtatcgtca aatccttgcc
    41521 tgtgtccccc ttttgaaaaa ggctggcagg gaaaacccgc acaaagcaaa tcaaaaccgt
    41581 aaggtattag atccttaacc tcctgtagag taatatcgcc aaaaggaaac tctccaaagt
    41641 tgaccatata ggttgcttgc gattcggtgt tccactcgct gctaaacacg catttaccat
    41701 taagctcctg catggccaaa cggaaaccac caattcctgc aaataaatca ataaatgtga
    41761 atgatggttg cttgggcgag ggaaagggaa ctttaacccc ctcatctaaa aggatcgcta
    41821 gcaaatcgct tccattgtca ggttctccat gttcaataaa attatgcatg ctgtagttat
    41881 caaaaatcct taccgcctct ccactatagc ctgctgaaat gttttgggta taattttgca
    41941 ggtagtgagt gatgactgcg aggtcaaatg attttcctct cttacaccat gaattaattc
    42001 gacttaaata gctattacta gaggcgtcca ttggttcatt gtatcgaata ggcaaatata
    42061 gagccaattc ttcaggatta acgcttgggc atatttgaaa tgtttttaga accgatattt
    42121 tgaatattcc ataggtttta atacttttgt ccggcatatt gaaggaggga taacaaccat
    42181 ctatcttata aagtttacct gacttgattg taagagttca gccagattan nnnnnnnnnn
    42241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    42301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt ggaaaattcg tgagttcagc cagattatac
    42361 acggagaggt gggtgagtgg ctgaaaccaa cggtttgcta aaccgtcgta cttcgcaagg
    42421 ggtaccgggg gttcgaatcc ccccttctcc gcaccatcgg ggtgtggcgc agttggttag
    42481 cgcacctgct ttgggagcag ggggtcgtgc gttcgagtcg caccaccccg accaggaggc
    42541 ctaaacggcc tccttttttt tgcttttatc gctgcatgtt ccattgttta tgaaaacaag
    42601 caactcgatg aatactagtt atttatgtgg atagccattg ccatacatcc agctggtgtc
    42661 agagcctggc ttgcttcaat ctgataaaaa aacgatattt gtcattctct tttagcgcaa
    42721 caccatgact gctgtttttt taacctgtta ccattaacgg taacagaaca tgtgtcattt
    42781 aagcaatggt tcatgaaaca aaggaaaaac aaggatgatg aaagtgcgct ttttgtggct
    42841 ccaggaattg accagccgcc tgttgtaaac gagcaggccg ttcaaaagtt taggcagcaa
    42901 aagcgccggc aaccgagtat agacgagttt gttggcggaa tacttgctcg caacaacacc
    42961 atcctgagcc aggcaattac attagtcgag agttcactac cacgacacta cgagctggcc
    43021 cagcagctaa ttgaacgttg ccttccctat tcgggtaatt cgattcgtgt tggaattaca
    43081 ggaattccag gggttggcaa aagcacattc atcgaatcgc ttggaacaat gctaacatcg
    43141 cagggacaca ggttggctgt tcttgccatt gacccgagca gtacgcgaag caaggggagc
    43201 attcttggcg ataaaacccg gatggaatcg ctcagcgccg atgccaatgc tttcattcgc
    43261 ccttcgccat cagccggttc tcttggaggc gtagcacgaa agacaaggga aaccattatc
    43321 ctttgcgagg ctgccggatt tgatacggtg tttattgaaa cggttggcgt tggtcaaagc
    43381 gaggtttcgg ttcactccat ggtggatttc ttcctgctgc tcatgcttgc aggagcaggt
    43441 gatgagcttc agggcattaa acgaggcata atggaaatgg ccgacctgat agcaataact
    43501 aaagccgatg gagcaaacat aagcaggtca aaattggcgc aggctgaatt tcaaaatgcc
    43561 ctacacctat ttcccatgcc cgaatcgggt tgggttccaa aagtggttac ctgctcggct
    43621 ctgaataaag ttggaattga cgaaatttgg cgtgagatgg tagcttatgc cgatttcacc
    43681 aggaaaaacg gctactttgt tagccgtaga aaccgacagg caaagtactg gatgcatgag
    43741 actataaacg aagcgcttaa aaacaagttc tacgccgaca ggttaataca tgaacaagta
    43801 agagaattag aaaaggaggt ggttgccaac aggttaagcc catttgccgc cgcacaactc
    43861 cttcttgacc ggtatttcag ccttaagtag ctactctggc aagcgggtca acttgagctg
    43921 ctcaaacgat gaggaatgga taggattatt gcaaagttga ttgacattgg ctatccgtaa
    43981 tagagtgagt aaaaaaattg cacaaccagt aaaacaaact atgcgcaaat ggataagaaa
    44041 cagtttctca gcaccatact aaaccgaaag agtgttagaa gttttacagg aaaaccgctc
    44101 gggcaggatg atatggagct tcttttacga gccggtatgg ctgctccctc tgcacataac
    44161 ctgcaaccct ggaagtttat agcaatagtt gacaggcaga tattgaactc ccttgccgaa
    44221 aaacttccct acgccaaaat gctttttgaa gcccctgccg ctatagttgt atgtggcgat
    44281 ttaaccaaaa caggaagcag tcccaaagat tactgggtgc aggattgttc tgcggcaagc
    44341 gaaaacattc ttcttgcagc tgaagcgcta ggcttgggtg ctgtgtggac aggtgtttac
    44401 cctcgtgcag aaagggttga aaccgttcag ggaattctta accttccttc cgaaattgtt
    44461 ccacttaatg ttatccccgt cggaacccca aaaggagaac accaggctaa ggataagttt
    44521 aaatcggaaa atattcagtg gaatcgctgg ccctaaaggc tcccaatcag ggtttccttt
    44581 gaaagagctt tgtacttttc gttaaagttc cggtaaacct ctgctatatg cttgtagtcg
    44641 tcgctgttcg gctcaatttt tagtccatgg ttgtccagct tatcttctat gtaggcaagt
    44701 gttaggtgat caatatgcct ggcaggctgg taggcgtagt ttttggctat gggcgttacc
    44761 acttcgttca gaaagccgca ctccaccatt tgggtgagaa gattcctcat cagcctctga
    44821 ggaattttca actgctttga aaggtccagg cttgtcaacg gctcttcccc attgttaaat
    44881 cttttgatga tggtattcag tattaaaatg gaaagaactt tcctgttgaa gtggcttatc
    44941 tggtcggttt ccttttcaaa ttcgtacatc tcgacattct ggtaggcaaa agaaacctcg
    45001 gcaccaaaaa gtacaattag ccagctggta tgcacccata ccaggaacag gggtagtgca
    45061 gcaaaactcc cgtaaatggc attatacttt gatactccta tctgtaggta aacataagcc
    45121 cattgcacaa taagaaacgc tgtgcctgaa agtatgccgg caattatccc cgaaacaatc
    45181 gagacctttg tgttgggaag cgtaataaac aaaaagctga aaagtatcca aatcaacagg
    45241 tagggtacaa gcttaaaccc cgaaagtagt accgggctta taatgtgtat gatttggctt
    45301 ttttcggcta ttgtgctcac ctgggtagaa aggtaaattt ggattccgct ggcagcaaga
    45361 agcagaatgg gcgctataac cataatcgaa aggtaatcgg taaattttcg cacgtaggtt
    45421 cggggttttt ctatctgcca gatgtcgtta aaggcgttct cgatgctcga tagcaccatc
    45481 accactgacc agaaaagtaa caccactcca atacccgcaa taattccacc gcctgtgcgc
    45541 tcaagcagcg aagacgaaaa ggtaatgagc ttgttcataa cctcctcctg tccctcaagg
    45601 ctgcttctaa tttgctgctc cagaaacttg tcaagcccaa aacccttggc aattccaaac
    45661 accattgcaa aaatggggac tatggacata agggtgtagt aggtaagggc cgacgcttta
    45721 acgctaacct ttttctcgtt gaaaccacgt agcgccagca gccccactcg aaggtacttg
    45781 tagaaggcct taagcttagg cgaaagctca tcaacctgta aaacccaaat atcatgggtg
    45841 atgaatgagt atatttgctc aaacttcttt ttaagccgct ggattctact ttcctttttc
    45901 tctttcttcg gttttgcagt ttttaccata accttacaaa ataaatttta taaaggcacc
    45961 tagctccggg agcaggtgtt aaggtactca cgatttttac tgccaaatat ttcggctcga
    46021 attattacat caaagtctca taatcatcca attgctaagg caaacacctt tcctggcata
    46081 cttctaacaa ccccagcaaa ttcaagattc agcagcagcg ataaaacctt agaaacctgc
    46141 aaacctgtgt ccatagcaat tacatcaacc gtttcctcac ctttttcctt tagaaagtta
    46201 agaatttttt gctcatctgc agaaaactta tcaaagttaa tagaatactg gcgtgatgaa
    46261 tttacctttg aggtggtcca gcccatcaga aattctaaat cgtcggcgga ctcaaccagt
    46321 gctgccttat tttgcttaat cagctgatta catccctgcg aatataccga gtttacatta
    46381 ccaggcactg caaaaacatc acggttatag gagttggcaa tatcggcagt aaccagcgca
    46441 ccgccctcca gcgcgctttc aaccacaagt gtggcatcgg ccatgccagc aataatccga
    46501 ttccgacgta aaaagttatt cctgtcgaat accgcatcgg aggtgaaatc gctaaccagc
    46561 gctccctggt ttagtatagc cttggccacg cttcggtgcg attgcggata aatggtgttc
    46621 aggccatgcc ccagcaccgc aacagtaggc aggttgcgct ttagtgccgc cctgtgagcg
    46681 gtaacgtcaa ttccataagc caacccactt ataattattg gcctataccc cttttctgcc
    46741 aatcccgaga ctaaatcctc acacatcgcc tttccatatt ctgtagcccg ccgggttcct
    46801 acaatgctta gcactttatc tgtgttaaaa tcaatgttaa tatttgattt tacgtaaaga
    46861 acaacaggac catcctcaca ttgctttagc cggtaagggt atgccgcatc agtaaaataa
    46921 agtactttga tgccgaattt ttcaataaac agtagctcgt tttcagctcg cgatagaact
    46981 gtttgggcta caatttcggc ggcagtggcc tcgcctattc ccggtatttt aagcaggctg
    47041 ccttttttct gcttaaaaac ggcttcagca cttccgcagt atgcaatgag ttttttggcg
    47101 ctaatacttc caacttttgg tattagttcg atgccgattt tatataccag ttcgggatcg
    47161 gcgctcattt acctttcaca tagctgttaa gcgcagcaac cagctgctca agttgttttc
    47221 cggtaagcga ggaaattgaa atagccggat acctagaaac tttatttccg ttccggacaa
    47281 aaatgctaag ttcgcgacga aggaactttt tctcgatggt gaagcggtaa aactcgctct
    47341 tgtcaacctc gatagaaatt ttgggtgctt taaatgggcg aagcagaaaa tacctgatga
    47401 tgattttttt cccctcatcg ttgtacgaaa agtagctggc tcgcctcagg tagaaatagc
    47461 ccgagaaaag gatgtagagt agcagaatga tggcaattgc ccaatcccgg tctatccctg
    47521 ttgtccttag taaaaaatcg ctaaggccgg tggctatcaa tagaacaatt aaaataatgg
    47581 ctatgatggc cattatgagt ttggccgaat ggataccagt tacggtgtcg tcgttatcaa
    47641 atttcatccg ttagccttat ttttcaaatt ttatccgaaa aattaatata catacggaac
    47701 tcaaccaagt tgttggctat gtaaaaatag aataatcatc caacatgccc aacatttata
    47761 tcattattta ataatgccaa cgcctcggtt tgtgagattt tagtttttag ggattctttc
    47821 aggtttctcc ttccaccaat ttttcccttc gctttacctc tttccttaaa aaatgacgct
    47881 cttccctcag gtggctggct gagtaatgct gaattatgct gctaacgatt cccttctcta
    47941 ctatattttt tggcccatag ggaattgttt taacaacatt gtagcctgaa agggcaaggt
    48001 taaagcttaa atgatcggct aggccaaggt gatgggcaaa aaatacttga gggtcgcctg
    48061 gactaacccc gaggttttcc attaaagata gaatgtagag aatggtgtca tggttatggg
    48121 taccaaatat aaatgaagca gaggcaaggt tgttgagtag aaatttgatt gcctcgttta
    48181 agctatggtt gatgtcattt tgagtatttt gaaacaatat cttctcagat tcgcccacct
    48241 cccggtgtgt tagtgtgcct ctaagaagtt ttgccccaag tatgtaacca ttcttggacg
    48301 caatccgaag ctgttcaact agcctttcaa acgtctccac catgtcaaac ctaaacgttt
    48361 gatacacggt tgccctgtct ccgttatgga gcatcatcat ttcgctggca atactatcaa
    48421 tagcgggttg ataatcaaca tgttcggcgt caataataat ggattttcct gaactgaaag
    48481 cttcgttgca aagtagcctt accctattgg cgaattctgt gtaaatacct ttgtctgcat
    48541 ggtttagcat ggcgttttga gataccagcc gtagtatcct gggttcaact aaagtagaaa
    48601 gtttaaccag cgtaaaggcg tggttaggaa aatccctggc aattcttata gcccttaggt
    48661 actcatcaaa aaccaactgc ttatcattgc attcaacaag gggcgctggt gaagagtaaa
    48721 ctccgtagcc ccatagttcg tctccaaccc tttcgcaggt ttccagcgtt aagcctccgg
    48781 aaaaaaagtc gaatattcta tttttgagca ggaagtttac gggaggaatt gatgcccacg
    48841 aattagcctt gccagaacga ttcattccac ccaattttga tagcagggtg aataacctgc
    48901 atgctctttt caactctttg tttgacaagt actggaaagc attaccggtg ttatcaagcg
    48961 ctatcatctg actgcaatat ttaactcata aagttaaaac aattgtggga atgtgtgaaa
    49021 aacagaaaaa acacctataa gaaaaagtgt tgaaaatggt ttgccgctcc ttggcagaaa
    49081 agaaacgtct aaatggccat ggatacaata aaagcgcccg atgttaattc gggcgcttta
    49141 ttactactag ctattagcta aaactacttt tccctattcc aacttacagc tgattgtaaa
    49201 agcttgccat cacttaccgc cagatcctca gcatcttgat cgcgttcaaa aattggatca
    49261 ggtgcgggtt gggtacattt aaaaataccc acataggttt ttttggccaa gtcattttcg
    49321 taggtgttga atgtaaccca gtagtaaaca ggaattccat caacctctaa tactgcatcg
    49381 tttgcgtact tcaactttag caaaatagtc agaccttcct ggagcctttg ccacgttaac
    49441 gcaatttttt cctcatcggt ggcaagttcg gcaaatcccg gcacgttgta ctggcttctt
    49501 gtagaaagcc taaggtcaaa atttttgtag tatgcgctgg ctccaaagta aaactcgtcg
    49561 ttaccatagc tgcttccgta ctggcgtcca taagtaaggt aaacataatc aactaacagc
    49621 tgcaggtcgg aggcactagg agtcaaatgt acggttggat caaaaatcca atgctctcca
    49681 gtgtggatga actgtcccga ctgctgcaca ataccattgt acaccatcca actccctgca
    49741 ctattctggt atttaccggc cactttggta ttggcgttcg gatagttgta aaccattatg
    49801 caggtatccc cgtcctgtgc ataggggtat ttttgggaaa ggtaggttgg aatataccgg
    49861 ctagcgggat tagtatttgt gaaggcgttc cctgtaagcc ccattaggct gtattcgaat
    49921 ggttgaacgc aaagcacccc ttttgatttt tcccacttgg agcctgtaaa cctatagtaa
    49981 tcattgtagt ttttgaaaag gtcggctgta acacttttcc ctttagccaa caccttggct
    50041 gtaataacca catcatctac ctcccatgtt ctacttgccg atgtggttga agtgtacaca
    50101 aatgcaatgt aaattttggc tttaggaagg ggaacatttc ccgaaggaca aaatacaaag
    50161 ttgcctgttc ccaggtcaaa actcaggttt gtccatgagg ccgcatttgg atcgccgcta
    50221 ccgctgtagt tggtcgaata cttcagcagt aaatcgtttc catcgtactt ccatgctgta
    50281 gtaaagttga gcgttgcgct ttgcacgccc gagaggtcaa ttgccggaat gataagccaa
    50341 tcctcatttt cgttgtttac tccaccggag taaccagata ttttggcaaa gtagttcccg
    50401 ctatatgctg aagcagccca cttttgcgcc cctttgacgc taacagtgtc aaacttatcc
    50461 aggttgttct cgttaaaatt ttccttaaaa agggtatcag tgcgtgttgt tgtgctagat
    50521 ggctcaaaat cagacgattt ataggaaacc aggcattgct ggttagctgt gggcgacgaa
    50581 attgcagctg tcagaatctg tggaatgaat tcctcggcag gcatggatgg tatgaaataa
    50641 ccggtatttg caacagcacc tcctaccgat tgatagttgt cggaggtaag ggtgtaaagt
    50701 gttgggctgc tgtaggcagt aaggtaaccg gtgttttcaa gattaacgtt gtaggtcaca
    50761 tttgcagtag agcctaggtt tagcgcaaca aatctgttgg caagcacttt cccgacatag
    50821 tttttggctg taaacctttg gttgaatgcc tgctggtcct taatcagctg tgccgatgcg
    50881 ctgtcggcat gattttgggc atcagccaaa gcgtacttgc ttgcattaga gtaatcggtt
    50941 gcatcaagag tataggtgat gttttcctta tagggtttcc tggcttcgtc aagattgttg
    51001 taggtgtcct ccatggggtt acatgcccac aaaaggccca acagcatggt aagattgaga
    51061 atccttttca tcggtgttat catttattgt ttatttaaat tcgtttggtt aaattattac
    51121 ggttatgcaa cggcttgcaa atcttcaaca tatcaactca actcctaaaa tttgcgcgtg
    51181 cactatccat cgtgttaaaa gttgattcta agtccggcag accacgttct accaaagcca
    51241 tagaaaacat acgatgaaaa ctcatcatga tatgttccat ccgttgcatc ggcaatatac
    51301 tcggtgtcca gcaagtttga cacgttgcca tagatggtag cattgaagga tcccattttg
    51361 aaacggtagt tggcgtctat atcaagaagg tagtagtcgg gcatttccca tgcatccacc
    51421 ttgctttcaa tagtcgttct ggcagttgga tcaaaatcgg catagttttt accaaaccag
    51481 ttaaagtcaa cgcccacctt tagctttggc agcacctcat agtcaacccc aacagctgct
    51541 gttatttggg cagagttccc cacatgaaca tcagcaatgt atgcgttata tgtaccggct
    51601 accgattggt cttcgttgta aagggtaaag ttaacatcat ctgtccaacg gtaatctcca
    51661 taggagaaca ttcccttaac attcagggta ttaattggtt tgtagtagca ttccaactcg
    51721 actccctgat gaagcgcatt aattcccaga atgttggcgg tttggccatt caatgatctt
    51781 acaagccctt tatccaacca gctggtacgg aacacgttca gcttggcatt gaaatttcgt
    51841 aagttaaacc catagcctac ctcggcagta ataatctttt cgtacttaac gcccttgttg
    51901 aaatcgttcg tattattcag gaaggagttg gaaaaaattg gggctttttt aatgtaccca
    51961 ccgttaataa acacattttg actttcactt atcttgtagt taaaacctcc tttaatattc
    52021 cagggcagaa agtcatgcca tggggatttt tgatttccag gagcatactg gaaaaaatca
    52081 accctacggt agttgttgct agcagcagca acagaaagga atcccgagta gctattcttt
    52141 atatactctg cctgtgcata aagtcctaac cacagcacct gtcccctata gttatagtta
    52201 acataatcgc ccttatgaag cggctgtgat gcaggacgat ttatgttctt attatcaagg
    52261 tagtaaaccc caccaagcaa atcgtcaatt ttatatgcat gtacaccgct gtagtaccgt
    52321 ccgtcaaaac cggcggtaat cttaaaatct gaaatagatg tggtaaaagt tgaaagcact
    52381 ccataccact gatggtcatt aattgcattg gctattatgg cttttgaacc ggttgttgaa
    52441 gcggcatttt gtgctataac ccagtcaaaa tcaaaatacc cctcgggagt gagcgcagag
    52501 ttatcataag gtgttccgct ggggtagttt aagctcaacc aggtagaatt cggaccgtag
    52561 actcttcttc cgcctccggt tgctattgag gcataggcct gggtagatag catggcgtta
    52621 tcactaattt tccagtagtg gtttaatgaa atttgcggtt tgtggtagta gttgtaggca
    52681 tacccaatgt tataaatatc gccattgcgg tacccgaaat ccgaattcca cttagcctcc
    52741 atggggtgat cgcggaattc ctgaatagta tgcttgttgc tgcgctggtt atgccactga
    52801 ggtgctccaa atgcattgaa aactatgcta tgcttactgt taattttttt ggaaacatta
    52861 acaaaatatg accaaccttc aaaattagta gcctgaacat agccattgcc gtatgtatgt
    52921 ccccccgaaa gagtcatagc ccaaccgtta tccaacatgc cggtggatac cgtaaaagtt
    52981 tgcttcatat acccatcgtt cccaatgccc gcatataccg acccgccctt ttcagcgtct
    53041 gttgacctgg tgataatgtt gattgttcct ccaaccgagg aaagggcaag tttggatgca
    53101 cccagaccac gttgaacctg ctggttggaa gtaacatccg aaagaccagc ccagttactc
    53161 cagtacacct tgccatcctc catgccattt accggaattc cattaattag cacgcctacg
    53221 ttgttggagt caaaacccct aacgtaaacg cgggaatcgc caaaacctcc accgcttttg
    53281 gttgcataaa ttgaaggcgt tgatttaaga atttcgggaa attctttatt cccaagcttt
    53341 tcggcaatga ttttgggctc aatggttgaa acggcaaccg gtgtggttcg gtcacgaaca
    53401 aacgacgaaa ccaccagcac ctcttccagc cctattgagc tggccgtaag ctgtatttgc
    53461 cctaaatcct tttctgttcc cgagggaatg gttacctcaa ttgttttatc ggaataacca
    53521 acaaatgtaa ccttgatggt tcctttctct acaggtactt ttaatacaaa agaaccatcc
    53581 aacgaggtta acgttccaat tgttgtgttt tcgactgcta ctgcagcgcc aactaacgct
    53641 tcgccactgt tgctgtcaac aacaacacct ttcacaatac cctgtgaaaa ggctactcct
    53701 gctgttccta ctatacaaac ggtaagtagg atttttttaa taactttcat catactgtta
    53761 tgaatttggt tagggttaag tgtagctatt gattgtgatc tacaattttg tttgcgtatt
    53821 gaagttgtga aaaataacct gatggcttaa gggtgaatat taataaaata ctaacctcca
    53881 cttctcgctt gcaggaaacc gacccccgtt taaccgaaag cgttgttcct ttagtgttta
    53941 agctgaattt ttgtgcacca aattacaaac atgaatttca aagtgtatta tacggctaac
    54001 ctttctttac catcaactaa aatttggaat aaactttaac agtaattcca atatggttga
    54061 tttattttct aggtcaagcc cttagaacat ggcgttttga aaacatttaa actatgaggg
    54121 taaatgctaa cacgcctaga ttcaaataat taaaacaaca tatatgagtc tattagtact
    54181 taatgcacaa tgcaaaaatt taagaggtta cgtttaactc ataaggttaa agcaatcatt
    54241 ggtgtgagca tataggataa gccggataaa tgattgggta aaatgataaa aaaatccgtc
    54301 cagaaaaccc ggacggatta tttattaagt aaacatggct tttagaaaat caatcttata
    54361 ccaagtagag caccccatgt actggttgtg ctgatgttct tgctgagggt tgttttagtc
    54421 tcaaattctt caattgtttt ggcattaagc cggaatgtag gagcggtagt tgcggttggc
    54481 agggcaaccc tggttaaagg acggacattg tcgtagctag ccagcgggtt gtaggtatag
    54541 gcgccccact tgtcgtttag catgttacca acgttgagca agtcaaggct tatttgcagt
    54601 gtatatctcc tgctggttcc aaagttggag aaaatatcct gcagcacctt tacatcgaac
    54661 ctgtgaagcc atggttgaac atcaccaaaa cgttctgcat acttgccctt acgatctttc
    54721 aggtaatcat taccgttaac gtaagcccaa aaagcatcag cctgttgttg tgcagtcatt
    54781 tcaacacctc ctgttggggt atagggaaca aacatggcca gagcttcgtc cttgtccttt
    54841 ggaacataga tcaggtcgga ggatatgcca tcgttattta agtcgttgga gtaggtatag
    54901 gaccagcgtc cttgctgaaa tcctctatag gctaacgaaa tagtagtggc aaacattcca
    54961 ccatactcaa ttctgtagga ggcatggcca atcaactggt gtggagtggc aaagtttgag
    55021 tagcttaggc caggattgtt aagacttccc acagcagtat ttgaggagta ggcggagtag
    55081 gcggcagaac ctgggttaga ggagatatcc ttggccatgg tgtaggtgta ggcaaacatt
    55141 ccggaaaaac ccttgctgaa gtttttggtt agctgggcag tgaatgagta ctggtaacct
    55201 ttgtcggtat tagaaagaac aacagcattg gctattgtgg aaaccacctt tctatcgttt
    55261 gctgttgccc aaaacggcct attatctgga ccaaccatat tgccagttgg attggcttcg
    55321 ttgatgttaa tctgctgcac ggcattaata tctttcccaa ataatgcctc accggtaaac
    55381 acaaagttat agggtagctc aacatcaacc gccaggttgg aacgccaaac ctggggcatc
    55441 ttaaagtctt ttgaaacttc agccaatccg gcattattag gcaatgttcc gggtgtttga
    55501 gggaaaagcg tagaattgct tgctatgaat tccttgaagt tgggattaaa ctgcaagccc
    55561 attaaattcg cattgccagg gccccatcca atctcgggtg attgaatcat acccgagttt
    55621 gttggctggt tggtgaacca aacgaagggt aaaagccctg taaaaatacc ggtacctcct
    55681 cgaacctgca acgagcgatc gcctttaaca tcccagttaa atcctaaacg aggggagaaa
    55741 agcagctgcg gatcgggcca gctacccaca tcaatctttt gactatttgc gaaggtaagt
    55801 gcagaaattg cagggttatc cttcatgtcg tcgaagtaga agggaagctc caaacgaatg
    55861 ccataggtaa cttttaggtt ggtattcacc ttccactcat cctgagcata aaaagctccc
    55921 attccaaaag taagctcagc tccgggagcg tcttcaccat tatacccgta ggttactcca
    55981 aaccctatgg gatcagcacc atttatgaaa tcatcaaccg atgcataccg gtagtaggtt
    56041 gtcccctccc ggatgtatga attcttaaag taaagccggt caaaggaaac cccaagggtg
    56101 agagtgtgtt tttttaggtt aattgtggtg ttgttgagca aacttaacgt attgttgatc
    56161 acgtcgttgt tatatgaaaa caactcgtat ccaaaggaca tatactgatc gccgtcctcc
    56221 catatatcta caaaggggaa cagctttgat ggagatgttc tggaatcctg aatgtgggtg
    56281 tagctaccta aaaacttgtt tgaaatattc gttaaaaagg tgctgtttag ctcaccagta
    56341 atcgaacgaa cagtgttctc aaaaccgtag aacgagttag agaaagccag ggattggcta
    56401 ctaattcttc ctgaatttct agcgttgttg ggaggtccac tgttaaagtt ggtttcaacg
    56461 tccgaagtac ccactacgtc gttatagcga atggtaaact tatgattttt gctgatgttc
    56521 cagtcaacac gcgctaaaat tttggtgttt ttgttctgga agggatcaaa gtttttatac
    56581 ttacccgggt catagttata tgtgctgatt agatgattcc ttaccctaat catatcggct
    56641 tcggttgtac gcgaaatcat ctgatcgggg ttggcaaccc catcggtgct gggtttccat
    56701 gtaaccccag gaatatcttc attttcaatt tctccattta taaaaaagaa cagcttatcc
    56761 tttataattg gtccgccaaa acttaaccca aaattctggc tgttcctgtc gtttgcccct
    56821 tctacgtcgt ttccgtcaac cttatcgccg gtaaatgatt ttgggcgaat gtaggagtaa
    56881 gccgaaccct taaaggagtt gtctccacta cgggtaacag catttatgct ggcaccggta
    56941 aactgggaaa ctctaacatc aaaaggagca atattcacag aaacctggtc aatggcgtcc
    57001 aaggatattg gctgtgcatt tcctcccgga agggggttgc tgcttaaacc aaaattgttg
    57061 ttgaaggctg caccatcaac agtaatcgtg ttaaaacgac catctcttcc aacaaaagag
    57121 tttccctggg cttgaggtgt taactttgaa aagtcggtga tgctgcggga aattgaaggc
    57181 agtgtgttta tgtcacccct gctgatgttg gtttgtgcac cggttctttc cgaactcaaa
    57241 ataggattcc ccattcctgc cgaaacgaca acctcctcta gggatgttgt tgattctttt
    57301 aactttacat tttgcaggta cgattcaccc aggttaaggt taatgtccgt ataaactccc
    57361 gtagagtacc ctacaaacga aatgcttaac ttgtagggtc caccaacacg caggttggtt
    57421 aaacgatagt taccagcatt gtcagtagtg gtaacatact gcgttcccga aggctcatgg
    57481 atggccacaa cagtggcaaa tggcaatgcc attccttttt catctactac cttaccgttt
    57541 atttccgaag ttgttacctg ggcgtttgcc cataagccaa caacggtaaa caacatggcg
    57601 ataaaaaacc tgattttttt cattaaatta aaatttggtt agtaatggtt taattattgg
    57661 caaataaatt aacacataag gtgcatgtaa aacaaaactt tatataatag gtttaacttt
    57721 tgctactgca ttcgaaatat ctggacttac ccaaaaatca ctttccaaca tcatactact
    57781 tgaatatgtt tcggatttgc agctggtaat acggatactt ttttgtcatg ccaaagatga
    57841 gtcaaaataa tctaacggct gatagctata tattaaaaaa tgattaagtc taaacggtca
    57901 aagggtttaa atctcttttt tgaacgaaat taaggttttg cccttcttct tgcctgaaaa
    57961 taactgcact aagttataaa aatatgcctt atagtgtttt caatgttaaa cttatctcag
    58021 cgtaattcta gagttttgga ataaaaacaa gccatttcaa aataagtgtt tgcattattt
    58081 ccatataaat ttattataat aggatttttt gcttggtgtc ttgaaatatg ctaacagtag
    58141 ccccagctac ctttttgcca cagcgctgat agcttctgcc tgtcgccctc aggcacctcc
    58201 cataggtcga agtcttcctg caacccttat agttccagca caaccccttc cctgtcattc
    58261 tgcaggttct catcagccca ccggtagcat tgcagaggta aagtttcgat tcctcaccag
    58321 aaggcttcct gttgtcaact gaaagggatt aaacccaaac ctccgacatg ttgctagcga
    58381 cggagctcgg ctaccacctc ctcgttgggt attcatccac agctatgcgc ctctgagaat
    58441 gctacacagc aattgttgat aaatttggca aatctactag tagcgttaac actcaacgcc
    58501 catctagtgg ttctaagtaa cgggattaaa ctaccggctt tgagatcata tccttaattt
    58561 gaagataccc aggcgactga ccctagaatt ttattcccat taccagaatg tcgtcaacct
    58621 gctcgttggt acccttccaa tcggaatggc tggcagccaa aatgttttcc tgttgggtca
    58681 tggaaagccc agcaatggat tttaaaaggt tgataagatt ttgcctcttg tacttttggt
    58741 agttggggcc gcctagctga tccgaaaagc catctgtaaa aaggtatagc atatcgtgtt
    58801 tgcgtagctc tacctggtgg ttttcaaaag gcatttcctt tgggtagtat ccaatggggt
    58861 ttcgtgtagg cctgaattcc agcacctcgc cattcctgat tattgttaac ggcatgttag
    58921 ctcctgaata ctcaagatat gttgaggctg ggttgaacct gcaaatagcc atatccatac
    58981 catccttggt ttcaacccgt ttacccgact gtttgaggga gttttttacg tttaccctta
    59041 actcctcaag aatttggctt ggagatatcg aactcttcgg ggtaacaata tcgtccagaa
    59101 gggttatgcc caaaatgctc ataaacgccc cgggtacacc atgccccgtg caatcagcaa
    59161 atgccagaat acctgcgttt tcttgaagct taaaccagta aaaatcgccg ctgacaatat
    59221 ctcggggaag ataaaaaata aaaaattcag ggaaaaatgc tgaaagttgc tccctggatg
    59281 ggagaatggt cgactggatg tgccgggcat actcaatgct atcggtaatc agcctgttct
    59341 gacggattat tctatcgcgc tggctaacca cctcatctcg ttgtgtttgg atttcttcct
    59401 tttgctgcga aatcaagcta ttactttgtt ccagaatttc gttgtcgcgt tttttcctgc
    59461 ggtagctcga taccagcacc ccaactaacc caataagaag aacagaaagc agaagttcag
    59521 acaagagaat aatatccctc ctttttaccc tttccttttg aagttcgatt tccttttcct
    59581 tttgaaccgt ttcatacttg gtgaggagct ctgccaggtt tttctggtta tccctggcca
    59641 gaatcgtgtc cttccacatg gtgtaaattt cgtagtactt tagggcttct tcaaaattat
    59701 ttaacttttt attcaggcta taaatttgtc cggccagcgt cattttaagg tttggctggt
    59761 taagagagtc aatatagctc aaggttttag cgtagtaact ttttgccatt gcgcttttat
    59821 tcaagctatc gtaagcacag ccgatgctca tcagacagag ggctgcatcg ttttttgcct
    59881 tgaccttttc aaaagcatcg aaggcctttt cctggtaggc tattgacttt tcgtagttct
    59941 tgagggcaaa ctctacttct ccaatattag caaggcattg ggccgattta tagtagtttc
    60001 cctggttggt ttcaaatttt tgagccctgc tgaagtattc gagtgccttt ttgtaatcct
    60061 ttttattctt ataaataacg ccaatgttca tgagcgctgt tgcctcgtaa tcggcccatc
    60121 ccagtgtatg gaacatgctt tgcgcctttt ggtagtactc caacgactgg tccagcttat
    60181 caaggcttaa gtacaggttt cccagcacgc cgtaaacggt tgcaactccc ttttggttgt
    60241 ttcgtttttc gaagatatcc agcgccttaa ggaaactttc aaccgccatg ctgtattttt
    60301 gctcgaacat gtaggtgttg ccaatatttc cataggcaga ggcaataagg cttgaatcgt
    60361 gagcgcttat ggccatctca agcacccggc taaagcgctc tcttgccttt tcaaaacttg
    60421 agttcacgtt aaaaataaag ccataagtgt tttcgcaccg aatcattagg gctgtgtcgg
    60481 aaagatccgt ggctagctgg tgggcaattt ggttgaacgc catagctgag tcagtatcga
    60541 tgtttatata tctttgagcc aaatctatgg ctgtttcggc ctttaccgat gtgttcggtg
    60601 cttcattcag ctgttttagc aggctatcaa tgtccgacct ttggccaaaa gtcgtaaaca
    60661 taggtagaat agcgagtatg gctgaaatta atttcctcat aatcagattt ttcaataaat
    60721 ttatgccttt ttacctatat gatgtaaaaa ttcaccataa atttttacat cctaactcag
    60781 aataaatctt ctactgcaga ttgctgcttt actgccattc caagccagga aaagctattc
    60841 taaacaaaac ctacttgtag aatgctcctc aggaacaatg ccaaactaaa aaatcggcag
    60901 caatcagagc tgcaataaaa ctttgatcct acaccattaa attttccatt ggcggctaaa
    60961 tcgttttaga tatttacagt atatttgtgg aaaaggctca aacacggtga agaaggtaac
    61021 ccttaacgac attgcaaaaa aggttggcgt atcgaaaact ctggtatccc ttgtggtaaa
    61081 cgggaaggct aggggttacg gaatttctgg acaaacctgc gacaaagtta tggcagcagt
    61141 tagggcgtat ggatacaatc ccaaccaaac agcacgaagc ctgcgtaccg ggatgagcaa
    61201 cactttgggc ctaattgttt cggatatctc caacaacttt tatgcctctt tggcaagaca
    61261 actggaggat ttggcctggg agcttggcta caacatgata ataggcagca ccgacgaaag
    61321 cgtgggcaaa gagcttaaac tccttgaaat ttttcgtaac cggcaggtgg atggaattat
    61381 tctctcctct tcgcagcaaa acccaaccga gctggaaact ttggtaaaat ccggattccc
    61441 gctggtactc attgacaggt actttcctac tgtacctata ccgtcggctg ttgttaacaa
    61501 caaggttggt ggagaattac tggcacagca cctgctaaat cagggttttc gcaacccagt
    61561 ggtactctcc ctaaccccaa gctacatttc atccgttgcc gaacgggttg agggtttcgt
    61621 aagtgttttt gagaatgcag ccattcatac tgaagtgcac aatattccct tcctctgcct
    61681 ggtcgacatg gttgaggatt ttttcacgac tcgtctccat gataagaata tgcccgatgc
    61741 agtttttgcc gttaacaata acctggcaac agcgactctc cgagcctgtc atgttcacaa
    61801 cattgttatt cctaaggata ttgcgctggt ttgttttgac gaacttcctt actttgattt
    61861 ctccaaacca tccatcacct ctataaggca acctgttgcc gagctgtgca gcgtagcatt
    61921 ttcgttgttg ctagggcaaa ttacaaataa cggggaaaca aaacccggcc atgtagaaat
    61981 gatgcccgtt gagcttttgg tgcgggaatc gtcaactcaa atttctgata ataatggatg
    62041 gcggtcacct taatgcaaag gacacaaacc tatgattgta aaccaactta aaccatgata
    62101 caaccagccc ataaaatgac tataattcac tttatatcag gtgtttcatt gctttttcat
    62161 aaatcgcatt tggcaatgtc gcagtacaaa tcaccagccg atttcatatt aaggtgtgct
    62221 aaatcagtaa ttttaaacac tcatgtaagg cagactgcac aaacacgcat gaataagcga
    62281 acgttacttg caagttcggc aaagtcaaaa tatgggggtt ccattgctct accccgataa
    62341 cgaaaagctg tgggctagcg tgcttgtaat taaaaaacct aaaaaacttt ttcagcttaa
    62401 accttgagtt atgaatagaa aaacatttac aattcttttg gccttcctat gtatgggatt
    62461 tggcgatgcc gttggaccat ttgtgggtct ggcaaaagag cagttcgagc tttcaaactt
    62521 tgaagcgcag ctaattgcct tcatgggatt catcatgttt ggctttttgt ctgtaccaat
    62581 gggaatattc caagatcgac gggggaagaa agttgtgctg atgctgggtc tggcagttgc
    62641 cctagccggg ttaaccattc cgttatttgg actgggcagc tatgcgttgt tcctcctgac
    62701 aattctgctt ctgggcgcag gtgctgcaat tctgcaggta gcaggaaatc ccatcatgcg
    62761 tgatgtttct gaacccggga tgtactcccg aaaccttagc ttcggccagt tcattaaagc
    62821 tattggatcg ctgtcgggtt ccattattcc atttgccgcc gcaaaatgga tgggggccga
    62881 ttggcaagtt ttatttccta tttacgtgtc agccctgctg ttaacgttgg tcgccgtggg
    62941 attcacgaag gtagaggagg agaagtccga taagaaacct gcaacactca acagttgctt
    63001 ttcgctatta aaaaatcgct ttgtcctttt aatggtgctg ggcattttta tctacgtcgg
    63061 aattgaaatt tgcctcagct cgggtgttcc tatcctgctt agcacccatt tcgggatcga
    63121 cctaaaaaca tgggggttgc ttggtaatgc ctttttcttc atcgccattc ttattggtag
    63181 gcttcttggt tccattattc tcagctggac aaaaccggca gcatttttca gggtttcatg
    63241 tctcattgcg ctaatgggta tcctgctcat tttttccaac aaccagatta ccacattggt
    63301 aggaatattc actaccggtc ttgggtttgc aaacattttc cctttgattt tttcaattac
    63361 ggtcgatgcc atgccagagc gggctaacga aatatcgggt ttaatggtta ccgcaatcgc
    63421 gggtggcgca tttattcccc cagttatggg cttggtggcc gatatgtcaa gcgttagaac
    63481 aggctttttg gttcctttgc tgggtcttgg atttattatt gttctatcat ttttaaaacc
    63541 tcaaccctcc agacaatgag ctactccgac gatagcagaa ttgtgctaac ccttgacgca
    63601 ggcggaacca acttcgtatt ttcggctcta caggggataa aacccattgc cgatacaatt
    63661 acactaccca gcaacggtaa caacctcgaa aagtgtcttc agggaatcgt ggaagggttc
    63721 accaaactag gagctaagct aaaagggaaa ccttcggcca tcagctttgc cttccccgga
    63781 cctgcagact atcccaaagg aattattggc gatttgggga atcttccagg attccggggt
    63841 ggcgttgccc tgggaccaat gctacaggag aagttcggaa ttcctgtctt tatcaataac
    63901 gatggcgatc tctttgcctt cggagaggcc atcgcaggtt ttctgccctg ggtaaacagc
    63961 atgcttaaaa atgccggaag cccaaaaaat ttctacaacc tacttggagt aactattggc
    64021 acgggtttag gaggcggtct ggtgacgaat ggtaagcttt ttaccggcga taactctgcc
    64081 ggaacagaag tttggcttct gcgcgatggt gtcaacccaa cctgctttgc cgaggaagaa
    64141 atcagcatta gggcagtgca acgaacatac catgcacata cgaggaaaaa tgaaagcaca
    64201 ctgctttcac ctaaagaaat tgctgaaata gcctacggaa aacacgaggg cgatatggtt
    64261 gctgccaagt tagcctacca gtcgcttggc gcctccctcg gcgatgctct ggcaaatatc
    64321 ataactataa ccgattcgat tgtagttgtt ggaggtggat tgtcaaacgc ctaccatctt
    64381 tttgcccctg ccatgcttgc ccagctaaat ggaagcattt caacagttac cggagagcag
    64441 gtaaaccgct tggagtcaaa ggtgtttgac cttgagaatg aagggcagtg caaccttttt
    64501 gttaagggtg aaaatcggaa aatcaaagtt cccctaagca gcaaagaggt tgactacgat
    64561 cctcttaaac gggtaggcat tggcgtttca cgtttaggga ccagccatgc cgttgctata
    64621 ggagcctatg tttatgccat taacatgctt gaccataaca cattacatag ccaggctatt
    64681 taatgcttta atcctccatc atctaataaa tttaaggcga acgcttaatg ctaatgtatg
    64741 aaagcaaatc caaggtaata ggagtaatcc ccaccccatg tttcagggtg ttggtttttt
    64801 tagacaaaaa atgcaaacac caacaagttt ggaataaaag taaactaggc cataataaaa
    64861 taacaccact tataaatacc tataaacaat gaaaaagccg caactattga cagttgtaat
    64921 gctcatcagc attgagctgt tttcacaggt gaaagacccc gtggattacg ttaatccatt
    64981 tattggtacc gattttcacg gacacaccta tccgggggca accgttccct ttggaatggt
    65041 tcagcttagt cccgatacaa ggctaaccgg ttgggatggc tgttcggggt atcattactc
    65101 cgatagggta atctatgggt tctctcatac tcaccttagc ggtaccgggg cttctgatta
    65161 tggcgatatt ctcatagccc ctgtttctgg caaacccgtc tttgaaaaca cgcggtattc
    65221 ttctccattt aaaaaggaaa atgaaatggc ggttgccggt tactactcgg tttttctcga
    65281 caagtacaag gttcaggctg aacttaccgc aacccgtagg gttggcttcc accgctacac
    65341 ctttaagaat cccaataaag cggcgctggt tattgatctc gagcatcgcg atagggtcat
    65401 cgaatcttgg ttaaaggtag tagggaagaa cgagctgcat gggtttcgcc gatctagcca
    65461 ctgggcaaac gaccagcagc tatactttgt tatccgattt tcccaaccct ttgaaaaggg
    65521 gccaaacttc gtggatgcca gcgaacagct aagcataacg aacggcgaaa atggtgaagt
    65581 tttggctgag ggaaccaggc ttaaggcatg gcttcagttt cctaaacttc caaacggtaa
    65641 acttcttgta aaggtcggac tttcagccgt gagcgaggag ggggctttac gcaacctgga
    65701 agccgaggtg cccgattggg atttcgatag cgttgccgct caggccaaaa acgactggaa
    65761 tatcgagctg tcaaaaatta aagtggacgg cggcactccc gaccagctga ccaccttcta
    65821 cacagctttg taccacgcta tggttgtccc caatgtgttc tctgatgttg atgggagcta
    65881 ccgtgccatg gataaccagg tgcaccatgc cgaaggtttt actccctata ccgttttttc
    65941 gctttgggat acctacaggg cttggcaccc gctaatgaca attattgata ctcgacgtac
    66001 taccgattat attaacactt tcctgtctga ttaccgcttc ggtggtcgct tgcccgtgtg
    66061 ggagctggct gccaacgaaa ccgagtgcat gattgggtac cattcggttc ccgttatagc
    66121 cgacgcatgg ataaaaggaa tcagaaattt tgatcccgat ctagccctga aggcaatgct
    66181 ccatagcgca aatctcaacc attttgggct aaaggagtac aggcagtacg gttttattcc
    66241 tggcgatatg gagcatgagt cggtttccaa aacactggag tatgcctatg acgactggtg
    66301 tattgctcaa tttgcgaaat ccattgccga tagctctacc taccaaaagt tcattatgcg
    66361 ggcacagtca tacaagaatc tcttcgaccc atccactcac ttcatgcgac cacggataaa
    66421 cggcggttgg aaggataact ttaacccatc cgaggttgac caaaacttca ccgaggcaaa
    66481 cagctggcag tacagcttct atgtacctca tgatgtttcc ggccttattg aactccacgg
    66541 cggaaagaaa aatttcatcg gtaggttgga tgagctattt tcaacctcat cccagctgat
    66601 ggggaaaaat caagttgata taaccggcct tattgggcag tatgctcatg gaaacgaacc
    66661 cagccaccat atggcatacc tttactgcta tgccgggcaa ccgtggaaaa cacaaaagct
    66721 tgtacgcaag attatggacg agatgtacac ctcaaaaccc gatggccttt gtggtaacga
    66781 ggattgcgga caaatgagcg catggtacat tttaagtgca gcaggatttt atccggtctg
    66841 cccgggaagt acacaactcg tcattggttc tccgctcttc agtaaaatta cctttaacct
    66901 ggaaaacgga aagtattttt cggtggtagc cgagaacaac tcgcctgaaa atatttacat
    66961 caactctgct tcgcttaatg gtaaacccat tacccgctgc tggattgact acaacgagat
    67021 aatggcaggg ggcgaactcc gtttagctat gtcgcctact cctaatagcg gctggggcag
    67081 caccgatgct gatatgccaa cctcatcaat tgatgagcat tttatagcta ctgctccggc
    67141 cttcaacacc tcaactaaaa catacatgga cagcttgcag ctaaccctca caactgtcca
    67201 gccccaagcc tcaacttttt attcggtggc agaaataaac caccccgaag ccattccaat
    67261 atggacacaa gggaatacgc tgaatcttaa gagctccaaa cgggttaaag cctactccgt
    67321 tcttccgggt ggcgaatcca gcgccatagt tgagggttcc tttatccgaa tcgagggaat
    67381 taaaaatgtt gagctgaaaa gcatccctag ccctctttac gatggtggtg gaaaacttgc
    67441 gctggttgac gggcaaagag gctctacaaa tttcaggttg ggaggctggc agggctacca
    67501 aaatcaggat tttgaggcaa ttgttgacct gggccatgaa cgtgaacttc actatcttgg
    67561 agcagagttc cttcaggatg cctcgccatg gattatgatg ccccggcaag ttaccttctc
    67621 tgtttcgtcc gatggtacta gctactccaa cattggagtg gtcaacaacg acatccccga
    67681 tactttagaa aagcccatag taaaagaact cggagtagct gttaatgtcg aggctcgcta
    67741 cgtaaaggta tttgcaaaat cgtatggaaa actgccatcg tcgcatgaaa gtgccggcga
    67801 atcatcctgg ctatttattg acgaaatagt aatccgttag ctaaatgttc agcttatgaa
    67861 cccacaagac gcataaacat accaacacac attttagccg ccaacctgct gaaccacaaa
    67921 atcatggtca tttaaggctg gtccttctcc tatttttttg taataatttt ggcttttgct
    67981 ttacttcagg aaatttttgt tgtatcttat agctgtaatc caacaccatg taaaatgaat
    68041 aatgatttta tctgtccaaa atgtgaggga tacctggttg taggaggcag cgtgattctt
    68101 tctattagca tgcaaagcgg attacgaggt cttatcctgc ttagtcctga acttggaaac
    68161 tatgccaagg tgagtagccc tcatgttaaa attgaaaatg gcgaaatgct ggactactac
    68221 tgtcccattt gccatgccaa gctggctgct tcagatgtac accctcgtct ggtaaggctt
    68281 atcatgattg acgacaaatc caagcggcat gagattttct tctcgggaat tgtgggggaa
    68341 cactgcacct acaagatttc ggagactgta tatgagaaat ttggcgaatc atctgccact
    68401 tacgatcagt acttcaaaac ccgtagaata taggctgaca tccatgtgta gaaaaatata
    68461 gcctagccag ggcggttgca ctcgggttat gcttcgtggt ttatctttca cgcgagacct
    68521 cctatggggc aaatttttga tggggcatgt tgtgttggta tactaaactt ccatacctaa
    68581 acctattgct attccctgtt gcgccggagt tataggggga ttatcattaa aaatgaaata
    68641 tgtaaagctg gtaactaggc aattccgggc ttgcctatct atccgaagat gccctgcctg
    68701 catttttaac tgaaagcaac atcggccggg ttgcgcccta agcgatgtat tcagttgagg
    68761 cgctcctcct cagtcaaatg gagaaattaa tacctcgaat aaatttatta tatttgtatt
    68821 ttggctagta tcacgacgtt ttccctgcag ttttaactag cattctgttg cttgcaacca
    68881 caataagcat ttttacaaaa tccgattccg ctcatttaac cctttacaaa atgataacag
    68941 taattatacc cacactcaac gaacaaaata caatctcgca ggtaattgaa cttgtggaaa
    69001 aatctgcaaa cgtatcagaa attttggtag ttgatgataa atctttggat agcacagtaa
    69061 aaattgccag ggcaaaaaac gtaaagattt atacaagcac aaaattgggt aagggttctt
    69121 caatgtatga gggaatgcta ctcgccacaa atggtataat tgtatattta gacgctgaca
    69181 taactaccta tcctgaaaat attgttaatt tactaaccga cccaataata aacaatactg
    69241 cagattttgt aaaagcaaca tttaataggc aagccggcag ggttactgaa ttagtggcaa
    69301 aacctttatt aagccttctt ttccctgaac ttgctcattt ttctcaaccc ttaagtggaa
    69361 tgattgcagc aaaaaaaagt attctggaaa aggctacttt cgaaaacgat tatggtgtcg
    69421 acgttgggct gcttattgat atgttccagg aaaaagcacg aattgtagaa gtaaatatag
    69481 gatatataga aaataggatg cagtcgtggg aacaacttgc taaaatgtct aaagaagttt
    69541 caagggcaat cttaaaacgt gcagaactaa taaaacactc aaatttggag acacttggta
    69601 attttgctat gattagagac caaatggatt ttgctattaa ggaaagtctg cttggtttaa
    69661 acaaaatgtt aattttcgac atggataaca ccattcttac aaaaagtttc attttaacac
    69721 tggctacaaa ttataaattt aaagaaaaat taattgaaat tgtagctgaa aatagtagcc
    69781 cttatgtccg aacaaaatta attgcacaat tacttaaagg tttaaaaata aacgaattgc
    69841 tggaaacatt agatggcatt cctactgtta atgattttga aagaattgtt aaggagttta
    69901 aacaaaaagg ttatatatgc ggtataataa gtgatagcta cgattgcata acaaatcatc
    69961 ttgtaaataa atttgatttg gacttttcta tcgcaaacga attagaattt tcaaacggca
    70021 tggcaacagg ggaagtcaaa attccttctg cgtttttacg tactaaagat agtacatgta
    70081 atcatgattt ttgtaaatcc aacgccatgc gagaagttgc acaccgctat catattgatt
    70141 tacacaatat aattgctgtg ggtgacgatg aaaatgatat ttgcatgata aaggattgtg
    70201 gaatcggcgt tgcgttttgt tcttcaaatt caacattgaa ttttatagca gatagagtaa
    70261 tccaagaaag aacatttgcc cctttgatag agcttctgaa ataaaaaata aatataaagc
    70321 tttgcttttt atcaatttaa taaagtaaat tctaaaaagg aggataagct tttagacatt
    70381 ttgctaccta tattgaaaag atgtcctttt tcaaaatata cccactccgt ttcaacatag
    70441 tccgaatccc gatggaatag cctcaaaatg gaacatttga gttcggctgc tgataacatg
    70501 cctagccaaa cccttaaaaa aatttttcac atcatttaaa atggttacct ttacccccga
    70561 cttttggggg tgcctttaag gctgagatta caccctttga acctgatccg ggtaataccg
    70621 gcgaagggaa aaagagtttt aggtatcaat ctcaagtttt tttacttccc ccttttttaa
    70681 aaggctactc aagagcatca accggggtgc acaccgctat tgcttttggt ggcccgatgt
    70741 ttaacactta aaaaagggta aaaaccatga aaaaattttt tcttttgttg ctcatgcagc
    70801 ttgcagctgc atgggctttt ggtaatccaa ccgatttttc aggctcatta aaagttagcg
    70861 gtagggttac cgattctgaa ggtaatccgc tagttggagc aaccgtagct cttgagggta
    70921 ccctgctggg aacctcaacc aaccccgatg gcagctttgt ccttaaccgc ttgcgacccg
    70981 gaacctatgt gctgatcgcc tcatttgtcg gtttcgaaac cgccaggctc aacctggtgc
    71041 tgcaagaaaa tgtagttatc aacattactc ttaaggagtc aacgctgatg ttcgacgagg
    71101 ttgtagtaag cgctactcgg gcttccggac gtatgccaat tgcccaaact accataagca
    71161 aggagcaaat tgacgggaac aacactggtt ttgatgttcc ttatctcctg gaacttcaac
    71221 cctccgttgt tgcaacctcc gaggggggga ctggcattgg caacacggct attcgcattc
    71281 gaggaactga tatgtcgcga ataaacgtta cggttaacag cgtgcccctg aacgatgccg
    71341 aatcgcaggg cgttttttgg gtcgatttgc ccgactttac tgcttcggta gataaccttc
    71401 aggtgcaacg cggggtgggt acttcaacaa acggtgccgg ggcatttgga gcaactatca
    71461 actttcaaac ggtttcccta tcgcaaacac catttacctc attcgacatt tttgcgggct
    71521 cctacaacac ctggaaactc tctgctcggt tcggaacggg tttggtaaaa gaaaaattta
    71581 gctttgaggg tcgttactcg cagcttggct ccgatggata tattgatcgc gcgggctccg
    71641 accaccggtc aatgttcatg accgcagcat ggcacggcgc taaaagtctg attagggtta
    71701 acatcatcca tggcgaggag cacacgggaa taacatggga gggcacaccc gattacatgt
    71761 tacagattaa ccgccgctac aaccccgccg gtgagtacac cgatgaattc ggcaataccc
    71821 gctactataa agaccagaag gacaactact ggcaaaccca ctaccagctt atttcaacct
    71881 tctccctggg ttctaactta acacttaacg ctaccgttca tctgacggat ggaaaagggt
    71941 actacgagga gttcaaggag gacagaaagt tttcaaaata tgcgctgcct aacctcgttc
    72001 ttggaattga caccattcgc acaagcgata tcatccagcg caaatggatg gaaaaccagt
    72061 tctacggtgc ggttgcctcg gtaaactacc gaaaaaaatc tcttgaagtt gctgtcggtg
    72121 gtggttggaa ccggtacgat ggcgaccact ttggccggat tctctggagt gcttataact
    72181 acgggttacc taaagattac gaatggtacc gaaacaacgg cctaaaaacc gactggaata
    72241 tctttgccaa atcggtttgg caacccggta aaatgcttag cctttttgcc gatattcagc
    72301 tgcgcggcat acaatatgac ctaaagggtc cggactcaga ccttctgccg gttgaccagg
    72361 aacatagctg gcattttgtt aacccaaaga ttggtacaac atttaggttt tctcctgcaa
    72421 gcgaactcta cgtttcattt ggagttgcca accgtgaacc gtcgcgaagc gacctcaagg
    72481 atgccctaaa gggcgcaaca ggctttgccc caaaacctga aagactttac gactgggagt
    72541 tgggatatgg ccttaggcat caggattttg cgattaacac aaatatttac tacatgaagt
    72601 acagggacca gctggtgcta accggtgagc ttacagatgt tggctatgcg cttatggaca
    72661 acgtccctga tagctaccgg gccggagttg agattcttct tggtttaaag ccatttcaat
    72721 ggctcaacgt ggaggccaac acaactttta gccgcaacat tattgaacac tacactaact
    72781 acgtcaattt aaccgataac ccaaccgatt ggaatgaaac agggttaacc gaagcagaat
    72841 atcttggtaa cacaaccatt tcctactccc cctccgtggt gggttcagct aaaattacgg
    72901 ttaccccggt taaagcactt tacttttcat ggattggtaa gtatgtgggc aagcagtaca
    72961 tagataacac cgaaagcagt agcagaagtt tggatgctta ctttgtaaac aacattgttg
    73021 gtgagtataa attttcaccc aaaatggcca aaagcatttc actgcaggtt gtagttaata
    73081 accttttcaa cctgaaatat gaggctaacg catgggtgta ccgcacaaaa tttcaaagtg
    73141 gcgatccgga gtatgtcagc ataggctact atccccaggc tacccgaaat ttcatgctcc
    73201 gacttgcggt tgggttctaa aattatatca acaataatga agagcatcat gcgatgataa
    73261 taggggtggg cagcagcaag cccgcccctg cttgccaaaa agattaggaa taagttgaaa
    73321 gaaaatgaat taattgaacc attaagcgtg agaagccaaa tgcttgtcct aaatctttag
    73381 aaggaacttg cccgtttgcc ggttgagctt gccgaagggt gaatcaacca gcaggcgtac
    73441 aagttcttga attttgaatc tgaaatcttc aattctttat ccctgaaata aaatgagctg
    73501 gctgataggt aactacatcg aacttctggg tgccaccatt ggcttgattt atctatactt
    73561 cgaaataaag cagaaaattt ggctatggcc attgggaatt gccacctcgg ctctctacat
    73621 atacatctac ttttcatcaa aattttacgc cgacatgggg cttcaggttt actatttgat
    73681 aataagtata tacggatggt accactggct gcatggaaat gttgacaata cctccagctc
    73741 aaagcccctg ccggttaccc gaataacatt gaggctacta gtagttctta cgactatttc
    73801 gcttttacta ttcgttattt tatggtttgt gctcgacagg tacaccgatt cccctgttcc
    73861 ggttggcgat gcctttacaa cggcgctcag catcactgcc acctggatgt tgacacgaaa
    73921 aattatcgaa cactggtggg tttggattgt agttgatggg gtctctatgg gactctacat
    73981 ctataaagga ctttacccaa ccaccgtgct ttttgctttt tacaccattt tgtcggttgt
    74041 ggggtactac caatggaaaa gggaaattac cccatctcaa gtaaaaacta ccgctggctc
    74101 ttccgcaaaa gagtaaattt gctagtccat ataactcaaa caagtgccct atgaacgctg
    74161 taattgttgc caacggcaaa tttccggaac accctaaacc gcttcgtctt atacatgaag
    74221 ctaaaacctt actttgctgc gatgggtcca tagagcatct tgttaaactc ggtataaccc
    74281 ctaccgccat agttggcgac cttgattcgg ttagccctga actcagggag cgtttcaaaa
    74341 acatactttt tcacgacccc gaccaaaaca gcaacgacct aaccaaggct gtttcctggt
    74401 gtgctgagcg taacatcaac tcaatttcca ttgtaggagc aactggaaaa cgtgaggatc
    74461 acacagtggg aaacatcggg ctacttgtta ggtatgccag aatgggctta caggttgaaa
    74521 tggtcacaga caccggcact atcaagcctc ttttagaatc agctaaatta accagctttc
    74581 ccggacagca aatttcaatc ttttctcccg ttaaccaaac tacaattacc acaaataacc
    74641 ttaaatatcc tattgataat ggccagattc ccgagtggtg gatgggtacg ctgaatgaat
    74701 cgctgggcag ttggttcgaa ctcaattttt ctcctggtcc ccttctcgtt tatcaaatgt
    74761 actaataaac tgtaatatat actagtttct gaatatcaat atattataaa ttttatttac
    74821 aatatcattg ctttttgatt gcatattgtt agctttgtgg tgggactaat taaatctcca
    74881 catcatgaag tatctaaatt ccgttattac cggaacaggt agctacatcc cttccgttaa
    74941 gattagcaac cgcgaatttg cccgtaacac cttctttaaa aaagatgaat cggcaattga
    75001 aaccctggtt gatgaggtta ttgctaaatt taaagatatt accggtattg aagagcggcg
    75061 ctgggtaacc gacgatttgt gcgcctcgca cattgcagcc attgctgctg aagaagctat
    75121 tgccactgcg ggcattgacc gcgaaactat tgaccaaatc atagtttcgc acaactttgg
    75181 ggatgttctt cagggtacca ttcagtccga caccatcccc agcgttgcat cacgtgttaa
    75241 gcacctgttg aaaattaaaa atcctgcttg cattccttac gatgttatat ttggttgtcc
    75301 gggttgggtt caaggggtca ttcaggccga ttcatatatt aaatcagggt tggccaaacg
    75361 atgcctggtg attggtgccg aaaccctttc gagggtcatt gacaagcacg accgcgactg
    75421 catgattttt gctgatggcg ctggtgctac cattgttgag gcggttgaag ctgacgaaaa
    75481 agccggggtc atagcctcct caatgatgtc gcacaccgag caggaagtct tctacctttc
    75541 actagggaaa tccttccatc ccgattcgga ttcccgcata cggtacataa aaatggatgg
    75601 tcgtaaaatc tacgagtatt ctcttataaa cgtaccaggt gccatgcgcg aagccatcga
    75661 aaaatcaggg attaacccaa gcgaacttaa aaaaattatc attcatcagg ctaacgaaaa
    75721 gatggatgag gccattgtag cacgtttcat caaacagcta aaaattcaaa cccccatttc
    75781 cgatctcatg ccaatgagca ttaaggagtt aggcaatagc tcggtggcaa ccattccaac
    75841 actttacgac ctaatactaa agggaaaagt gcccaaccat accatagcca aaggcgatat
    75901 cataatgttt gcttcggttg gtgccgggat gaacatcaat gcgattgttt accgacagta
    75961 atttagcaac acacgatgaa cttttgactg aaaataacca gctaacgcaa atggctaatg
    76021 cttgttttgc aggcctcttt tatggtggtt attccagcat aggttttttc ctttgatggg
    76081 tagcttgctc gtaggagtag gcaatgctta atagttccgc ctcgctccag gctttcccga
    76141 taaacgaaat atttacgggg aggccatcta taaatcccat aggaacgctt atgcttggat
    76201 aaccagcaat cgctgcatac gacgaacttc cccctaaaaa gtgatcgcca tcaaccaaat
    76261 cggttttcca ggctggtgaa ccagtaggtg caactagtgc atccagcttg tattcgttca
    76321 ttatacggtc gattccttgc ttgcgcatcc cagtttgcat catctcaaga ttttttttgt
    76381 actcggggct gttaagatct ccttttgatt gtgccatttc cagtagctgc tggtcgaagt
    76441 acattaactc cacagaatcg ttcaggttaa acttaatcag ttcgctaata ttctttaccc
    76501 gagcgctctc ccccaggctg gcaaagtagc ggttcaaccc atctttaaac tcatacagta
    76561 gaacctcgaa cgaagcgtta tcaacttccc tgcttaccgg aaaatcaata gctaccactt
    76621 cggctccagc ctgcttcatg gcgtcaacac tttgtttcat aagggtgtca accttaaaat
    76681 gaaatcctga tacgttggta agcaaaccta ttcgtttacc tttcagccca tctcttttta
    76741 aaaattttga atagtcagaa tagaataccg catccgggtt tagggttttt gcatcggtag
    76801 aatcgacacc caccataatt cctaaacata ctgccacatc ctcaactgta cgcgccatgg
    76861 gtcctggtgt gtcctgggta aatgaaattg gtataattcc cgtccggcta atcaggccta
    76921 ctgtgggctt cagcccaaca atgccgttgt tattggacgg gcacactata gacccgtttg
    76981 tttcggttcc aattgcaaaa gcacaaaggt tggctgctac ggcaacccct gaaccggaac
    77041 ttgacccgca tgggtttctt tcggtatcgt aaggattgcg ggtttgccca ccaactccgc
    77101 tccaaccact tgatgagaga ctggctctaa agtttgccca ttcgctaaga tttgccttgg
    77161 caataataac tgccccggct tcgcgaagtt ttttggcaac atggctatcc cgccaaacgt
    77221 atgaattctt taagacaact gcaccagcag tagtcggcat tgaatctttt gattctatat
    77281 tatccttcag tattaccggt atgccatgca agggaccacg gcttttccct gcggctagct
    77341 cgctgtcgag ctcctgggct attctcatgg cctcgggatt aatagcaatt attgagttaa
    77401 gaacgggacc atttttatcc agctcctcta ttcttgtcag gtaatcgttt accacttggg
    77461 ccaccgtgaa ctttccctcc cggtatccct gttgaagctg ttttatagta atttcctcaa
    77521 ggtattcggt ttcccctact tttgcctgtt cccgctcgtg acaacccgta gctaccgcta
    77581 taagtatgac cagcccggct agacatgcgg caatttttgt caacttttcc atttttgcaa
    77641 attttttaat agtgttagga cttgcttttc atatctactt tacacaaaca atgctgtgct
    77701 gagtgttgct gcggcaccct ttttcgtatg gcataactat gtcatatgct ataagtatgc
    77761 tttaattttg accaagttag ctcaactagg tttaaaatca aatacatatc cagggtgggg
    77821 gtaataggaa aaggcggttt gctgaaccgc cttttccgaa ttatcccagg ttaaacgctt
    77881 tactttaaag aaaattcaga gtagccaatc atatagccat ccatatacag tgttacctta
    77941 taaacgcccg tcaaaagttc tccattgtta tcaaaaaata tgcacacatc aagatcgctg
    78001 ttctggtaat cgacctccct acttgccgag aaaacaattt tttcgccctc aaaatcgaac
    78061 aaatcggctt ccgacttggt cagcacaaag tcatcgggtc caacaatccg catgtaaaca
    78121 ttccgggttc ccggtttagc aatagaattt tcgcttaggg taaagcatgc gcgaatttta
    78181 ttcaccctgc gtgctcgggt tacctcgttg cctttctggt ttacacccat ggctacaata
    78241 tcgcgtgcct taactattga accctttgca acctgggtat tgagctcttc cgttttttgc
    78301 tcaagctcta ttactgtttg tttggcggtg gctgcctcct ctttcacctt aacattctct
    78361 gcaattaact ccttgttcaa agcattcaag gaatctatat catggagcat atccttcata
    78421 attgaacgta gggtgcctag ctccttttgg tactccttaa ttttggcata gctaatctgc
    78481 ttcacactct gaagctcggc atacaacttt ttggcatgtt cctgctcctt ttgaagtttt
    78541 tgatttagcg tgtcgttggt agtctccagc tcgctgtagt cattcatcaa cctcagcaaa
    78601 ttattagaaa ttgagtcctt ttgtgcctgg agtatttcct gtgtatcttt tgcttgctgc
    78661 ttttgctgga agtacacaac agttaatcca gctataatca acagaagcat aaccataatt
    78721 actcttagag ttttaatggt tttggctgat ttttcattat tctccatttg tcttttatta
    78781 tttagttaaa actgttaggt ttagtgctag tttctgttac caagggtaag attttgaaat
    78841 acttcctcaa gttgaaggtc tcttttttga agggtaagta gcgtgaatcc tgcatttttg
    78901 gcaaaattaa atattttgcc tcgtaagtct tcgagcctgc cagaggtaat ccaccagcag
    78961 ttagtatcaa tgagggttgc cgtttccacc ccttcaatag ccatgatagc ttcacgacta
    79021 accttttcgg caaactctac aactatgttt tgcctaagcc ccgacgacaa agctttaact
    79081 tcggtattgg ttccatcggc aactactaaa cccttgttaa tgattatgat tctgctgcag
    79141 attgcttcaa cttcttgtag gatatgggtt gagaaaataa cagtcttgtc tctgcctatt
    79201 tcttcaataa cgttacgaat ttcgacaatc tgattaggat ccaaaccgct ggttggctca
    79261 tcgagcacca gcacggaggg gttgtgaatt aatgcttgcg ccaagcctac ccgttgcctg
    79321 taccccttgg agaggctgcc gatcttttta ccctgctcat cgcctatacc cgtaatgcca
    79381 ataagctccg ataagcgccg gcgcttttcc tttcccaact tgtagatgga ggccacccac
    79441 gataggtatt ccttcacgta catatccgta taaagaggat tattttcggg caaataccca
    79501 atctcccttc taaaatcaaa tcgggtatcc tgcgtgttat gaccattaat atcaacactt
    79561 ccgctgtctt ggaaaaggta gcccgtgata attctcatca acgttgactt tccggcgccg
    79621 ttcggcccta aaagtccaac cacctgacct gttgaaatat tgaacgaaac atcgttgagc
    79681 gctttctgct ctccaaactg cttggttatg tggcttattc ctattgacat cgttatacta
    79741 aacccgtaaa tgctagttct tcgttcaaat aaacggaatt gggaatgggt ttattttatt
    79801 ccaaactcac ttttaagcct atcaacatag tcgagctttt cccacgtaaa aaattccact
    79861 tcccgcttaa caattttttc tccgttgcag ttgctattct caataaaatc aacattacgt
    79921 ttataatggt cgcgcccaaa atgcccgtag gcggcagttt cttcaaatat gggattttta
    79981 agtcccagcc gtttaactat tgctgctggt cgcagatcga aaatggtcaa tattttttgg
    80041 gcaatttcac catcactgag gcttacttta gctgtgccat atgtattaac atatagattg
    80101 ataggcctgg ctactccgat agcgtacgct acctgtatta gcacttcgtc ggcaatacca
    80161 gctgccacca tattcttcgc aatgtgacga gcggcataag ctcccgacct gtcaactttt
    80221 gaagaatctt ttcctgaaaa agctccaccg ccatgcgctc cttttccacc gtaagtatca
    80281 acaatgattt tcctgccggt tagcccggta tcgccatgag gcccgccaat aacaaacttg
    80341 cccgttgggt taacatgcag aatataatcg tccttaaata gtttttgaac ccgctcggga
    80401 aaacgtttct ttgctcttgg aatcagaata ttctttacat cctcgtaaat tctgctgagc
    80461 atacgggcat catcggtatc aaattcgtcg tgctgggttg ataccacaat ggtgtgaatg
    80521 cggacgggtt ggttgttatc ttcatactca agggtaacct gcgacttagc atcaggccga
    80581 agataggtca tttgccgccc ctcacggcga atgccggcca gctccattag tagcacatgc
    80641 gaaaggacta aagccaatgg catatagtca tcggtctcgc ggcacgcata gccaaacatc
    80701 atcccctggt cgccggcgcc ctgttcctcc tccgactggc gaactacacc ccttgttatg
    80761 tcgggcgatt gctcatggat tgttgaaata accccacacg attcagaatc gaaccggtac
    80821 tggtcattcg tgtagccaat cctttttatc acccttcggg caacctcttg gacatcaaca
    80881 taggccttag ttttaacctc tccgctaagc actaccaagc ccgttgttac gagggtttca
    80941 catgccacct tggcctccga atccctccgt aaaaactcat caaggagagc atctgaaatt
    81001 tgatcggcta tcttatcggg atgaccctcc gatacgctct cggacgtaaa taaatacccc
    81061 attttaataa aatttagatg gttacttaat taaataacaa gggaaggttt gaacagttgc
    81121 ggcaaacagc acgttttagc atttattatt gtggttgcaa tcagtcaaat ctctccacat
    81181 tctaccggca aagataccac attatttatt tttactctat aatttatgtt cctctaatgg
    81241 gcaatatcca agttgaagta tataaaaggc ttttgtttag tctatcctga tggatttacc
    81301 caaaaattag acatttggct aaactaacct tctggttaag cggctaatcg gtatgtatag
    81361 ccatgcagcg attagcgtac ccacaacatc agctatcaaa tcaaaccagt ctgcccctcg
    81421 ataggtgaat actttaagct gaagcagctc aaccaatcct ccatatatga tggaaacaac
    81481 tatcccaacc cacctggtct tttgcaatac gcttcccgat agtctaagcg tgttgttttc
    81541 cgagatcacc agaagtgtta gcacggcaaa aaggccaaaa tgaaccaact tgtctatacc
    81601 cgaaacgctt acctttggaa atccggaggg tggcattgtg catagaatca aaataaccaa
    81661 cccccaaaga atcgaattcc tattcttgct gaaaaatttt agtgccttcg ccatcattac
    81721 cagtatagtt tggttgtcat tcttgtagca agtataaagc tagtaataat ttggtaaaca
    81781 aattgaacca tacctgcggc tctgcctttt accccttacc accaacgaat aaaatattgt
    81841 attgctattt atcaacaccc caatgaatta gaatctattt ggcttccgta ttaaattatg
    81901 ttaaattatc tattgttgac cctgtaaaaa ttgctaagca ccttgtagaa taagacttct
    81961 atagacttat ctacaaaatg acaacagagc ttagtttgat tatatcgggc aagtagctat
    82021 ttttatacca taaaattact aaaactcatg gccatgtttg acaagcagat ttatgttgac
    82081 cgcaggctta agctgcgaaa aaaggtaaaa tctggcatag ttcttatttt gggaaatacc
    82141 gaaagcccaa tcaactacca gggtaacacg tataactttc ggcaggatag taattttctt
    82201 tacttttttg gacttgacac cccggggctt gctggggtaa ttgacattga agagggtacc
    82261 gattgccttt acggcgatga ttttgacatt gacgacatta tttggatggg accccagcca
    82321 aagttgaagg atatgggtga aaaggtaggc gttctttcta cccatccatt tagagaactc
    82381 tctactacct tgaacagagc cataaggcag ggccgaaaaa ttcatttcct cccgccatac
    82441 agggcagata ataccctcct cctggaaaaa ttattgggaa ttaaccaaac cattattaaa
    82501 acctactcct ccattgaact cataaaagcc attgttagcc ttcgctcagt aaaagaaccc
    82561 tgcgaggttg aagaaataga aaaggcgtgc tctgtgggtt acaaaatgca tgttaccgcc
    82621 atgaaaatgg caaagcccgg cgctttggag cgcgaaattg ctggcgaact tgaaggaata
    82681 gctcactctt tcggatatat gacctctttc ccaacaattc tctcccagca tggtgaaact
    82741 cttcacaacc acgatcactc acttaccctt gaggaaggac gattattact ggttgatgcc
    82801 ggagccgagg ggattagcca ttacgcttcc gatttcacca gaacttttcc ggttggcggg
    82861 catttttcac aaaagcaaaa ggatatttat tctatagtgc tggctgccaa caataaaact
    82921 gcagaactag caaagcctgg cgttacttac ttcaacgtgc ataaggctgc agcaagggtt
    82981 attgttgagg gtctttctgc tcttggaatt atgaagggga attccgacga ggctgtagaa
    83041 aatggcgctc acgcactttt tatgccccac ggattaggac acatgatggg gcttgatgtt
    83101 catgatatgg aggacctcgg cgaagacttt gttggctacg atgacgaaat ttcacgctcc
    83161 gagcaatttg gaacttcttc acttcgcatg gcccgcaagc ttcagaaggg ctttgtcctg
    83221 actaacgaac ccggcatcta ctttattcct gcccttatcg aaaaatggaa atccgaaaag
    83281 attaatcagg catttatcaa ctacgatgta ctgtccagct atctaacctt tggaggcatt
    83341 cgcctggaag acgatttact aattaccgat aacggatgcc gtattctagg ccaccgaatc
    83401 cctattaccc ccgaagaggt tgaaaaaaca atgaattcat agcaaaattg ctgcatagcc
    83461 gattcgaaag gagcgttggc aacatccacg ctcctttttt gtttcataaa tagatagcgg
    83521 acgtagtttt gcatgtaacc tccactaccg taatgctcat tacggtaaaa atccagttaa
    83581 catcttaccg tttactgtta agttctttat tattgactgt tgaaaactag taatgctttt
    83641 ttgttgtcat gcattccaat ttaactactt aataaattct cgatacgtgt caagagtatc
    83701 ttacaaatct atacctgaag ttttaaacag cttagctaac taaatcgggt ggtttaattc
    83761 ggaaaatgtt ttaacattgc ccggcgttac ccgatgttaa ttacttcttt tatttgctga
    83821 ttaccagcaa taaccatctt ttctttgtgt taataatttg ttatttgcct tttaataccg
    83881 aattttgcat tattgttttc cgttttttac caacaatata aacaacaaat aatcataatt
    83941 taaatttttt ttaaatactt aaataaatat taatgaatta ttctattgtt gataagttag
    84001 ggtatattga tgaagatatt gatagcaaca ttgacttgaa aattgctata cgcaaaatca
    84061 aggaggaaaa aaatgcggtt attcttgcgc actactacca acgacccgaa gttcaggata
    84121 ttgccgattt cgttggtgat agcctagccc tttctttaaa agctgcaagt cttaatagta
    84181 gcttaatttt gtttgcagga gtgaaattta tggctgagac tgtaaaaatt ttacgaccaa
    84241 attcacaagt tctaatacct gatctaaatg ctggttgtag tcttgctgat tcatgtcaat
    84301 tttctgattt taaaaaattt atcgatttac acccaaatca tattgtagtt acctatgtta
    84361 actcttcagc tgaggtaaaa acgatttctg acatttgttg cacttcatct aatgccataa
    84421 aggttgttaa atcgttaaat aactctaccc cggttatttt tgcacctgat ataaatttag
    84481 gcagctacgt aaaatctacc ctaaacagat ctaatatggt tctatggaac ggttcttgcc
    84541 atattcataa tcagtttatg ttagaggata ttattgctct taaagctaaa catcctaatg
    84601 ctaaagttat agcgcatcca gaatgccaaa attcaatttt acttatttct gattttattg
    84661 gttcaactaa aagcctcctt gattttacat acaattcaaa taatactgat tttattgttg
    84721 ttactgaacc aggtataatt caccagatga agaaattatc accacaaaaa aatttctatt
    84781 tcccctatgt tcaaaatgaa gaatgttatt gtaatgaatg tagctatatg aaattaaata
    84841 acttaaaaaa aatttactta actttaaaat atgaatatcc aattataaat ttagaacccg
    84901 atgttatttc taatgcagct attcctatta atcgaatgtt aaaaattaac tagttaaaca
    84961 tatggaaaat caaatttccg acaaagaatt tgaaaacaat attaggccat ccgaattcaa
    85021 acaatttaat ggtcaggatg atataataaa aaaccttaaa attttcgtac aagctgccaa
    85081 aattaggtct gaatcattag accatgtgct gctacatggt cctcctggac tcggaaaaac
    85141 tactctggct tatattatag cgaatgaact tggagtttct ataaaaacta catcaggacc
    85201 tgttcttgaa aagcctggcg atttggccgg aatacttaca aatttagata aattcgatat
    85261 acttttcatt gatgaaattc atcgtttgag cccaatagta gaagaatacc tttactctgc
    85321 catggaggat tttagaatag atattttaat agataagggt ccaagtgcca gaagcatcca
    85381 aattgaattg aatccattta ctcttgtcgg tgccactact cgaagcggtt tacttactag
    85441 tcctcttaga gcacggtttg gaattaattt tcatctggag tactatgacg ctagtactat
    85501 aaaatctatc attaaacgat cctcttatat tttgaatatc gatatagatg atgatgcttc
    85561 tatggaaatt gcactcagga gtagaggaac tccccgaata gccaacagtt tactacgtag
    85621 agttcgcgat tttgctcagg tggtaggaaa cggccgaatt aatcttgata tagcaaaaag
    85681 ttctttagat gctttaaaaa ttgattcgtt aggtcttgat gaaatggata ataagatatt
    85741 aacaaccata atcaataaat ttaatggtgg acctgtagga ataaatacta tcgcaactgc
    85801 tattagcgaa gaccctggaa ctattgaaga agtttatgaa ccttttctga ttaaggaagg
    85861 tttactaaag cgtactccaa gaggaagaca gacgacagat ttagcatatt cccatttgaa
    85921 aattgaaaaa aattttaggc agggaaccct attttaaaaa tgaattagaa atactttcta
    85981 gtttagaata attaccaatt cctatttttt taccctttaa gctaattagc ccatctgttt
    86041 taaactcgct tagtattctt ataactgatt ctgttgccgt tcctattatg cttgaaatat
    86101 cctctctggt gaggttaaca tttataaatc cctcctcatc cttaccaaat gtttgatcaa
    86161 gcattagtag ggtttctgct agcctttccc ttactgtttt ttgagctata tcttttatca
    86221 ggtcattggc agtattcaat tccttacagg ttagctggat tagtgataag gcaaaatttg
    86281 aatttttcct aataaactca aatatcaagc tggatggtat gtagcatact tcgctatctt
    86341 ctagtgattc tacagtagta catgctggtt cattactcaa aactgatctg tagcccgtta
    86401 tatcgccttt ttgagcaaaa gctactattt gaggttttga gtctattccg gtcttataaa
    86461 ttttaaatac tcctttcttt acacagtaaa accctgtaat tctgctacct tctttataaa
    86521 ttatttctcc ctttttgtat tcgtaacatt ccaaatttgc ttctagttgt tctgattctt
    86581 cggtagtcaa agaaccaaaa accgttttcg acgacaggct acaggttttg caaacgttca
    86641 tgcttttcat tacctaccaa atttttgcct atagaaaatc agtaaaaatt cgtaaattga
    86701 tttaagaacc ttatatcatt ttcaaaataa tttcttaagt cattaaccct aaattttaac
    86761 atggctattc gctcaattcc cataccaaat gcaaaacctg aatatttttt actatcaatg
    86821 ttattaagtt ccaacacatt cggatcgacc attccacaac ccaaaatttc gagccatcct
    86881 gtatgtttac atacagaaca tcctttacca ccacataaat tgcagcttat atctaattct
    86941 gctgaaggtt ctgtaaatgg gaaatatgat ggcctcaatc ttatacttgt tttacttcca
    87001 aacatctctt taacaaagaa ctctagtgtt tgtttcaaat cggcaaacga tacggtttta
    87061 tctatataca gaccttctac ctgatgaaat atgcagtgtg ctcttgatga aattgcctca
    87121 tttctaaata ctcttcctgg acaaattacc cttattggca atttttcatt ttccattgat
    87181 ctcacctgaa cggatgaagt atgagtacgt agaagtacat ctggattttt ttgtataaaa
    87241 aatgtatcct gcatatcacg tgctggatgt tctggtggaa aattcaatgc tgagaaaaca
    87301 tgccaatcat cctcaatttc ctgtccttca tatatcgaaa agccaatttt cctaaaaata
    87361 ttgaaaattt catttttaac tattgttatt ggatgtagtg ttccgaagtt tagctgaggt
    87421 tcctgaagtg ttaaatctat tttatcttct ttttttcctt tagatttata tgtatctttt
    87481 aaaatatcta atttttcatt taactttttt ttcaagtcat ttagcaattt cccgtagtat
    87541 tttttttcat cagtgcctag attctttaga tcgtcaaata gttttgtcaa ttcacccttt
    87601 ttccctataa ggctaattct aaaactttca acttcctcat agttttgagg agtaaatttg
    87661 tcaattaatt ctaaaatatt atttattttt tcctgcatag ttaaaaatta tttagattta
    87721 acgtattttt ttgacttcgt ttattacaat atcaaccagc ggtctgtcgt tttgatcagt
    87781 ttttacctcc gatatttttt taacaatgtc catacctttt gaaacttcac caaaaacagt
    87841 ataatttcca tcaaggtgtg gtatgcctcc tatcgtggtg tatatctctc tctgtttatt
    87901 tgtaaatata aagggttttt ctgacgattt tacagaatcg accatgtttt ctattagaat
    87961 tagaatatct ttaatattct tatccgaaac agctgtattt tcattcaggt attttttata
    88021 taaatcttgt tttattttat ttaatttttc aaaatttatt tttttctcta aattatcgag
    88081 ttcctcgttt gtgtaaactt ttcctataac tatataaaat tgcgagcccg atgacaattt
    88141 atctggatta atatcatctg actctctagc cattcctatt gcaccatatt tatgaataat
    88201 agtatcgcaa aattcgggat caatcagata tcctgaatct ttttccccat attctttacc
    88261 tggttgggaa tttttagttt cagggtcccc tgcttgtatt acaaaatttt taataaccct
    88321 atggaaaatt tgcccatcgt agaaatgttg gtccgtaaga ttaataaaat taagtgtatg
    88381 tttaggtgtt tcgttgtata gtacaacttc aatatcacca taatttgttg aaattttaac
    88441 aagatgtcga ttatcaactg tggcattttt acagcctgtt agtaggagca aagcagctat
    88501 tgagaaatac agcaaaatat ttttgttcat agtagtaata ttaaaatttg ttcaatatta
    88561 atgaagtaaa ttccaaattc attcattaaa aaaatttagt tgaataaatt taagattgcg
    88621 cgcatatagt actttatatt tcgcaaaatt atatgaaata ttttaatttt gtgcgtcaaa
    88681 ttttatttat ggcttctgtt cgtcaacttt actcacaatc tgttgtttat ggtttaagca
    88741 ctgttgttcc aaggctgctt aactatctgt tggtaccact tcacacaact gttcttacta
    88801 atcctgaaca gtatggggtg attacagaaa tgtatgcgta tatttcgttt attcttatac
    88861 ttttaacttt tggccttgaa actggttttt ttagattctc ttccaaattt aaaaataacg
    88921 agtctgttta ttctactatt ttttattttt taattttcac ctcttcatta ttttttttaa
    88981 taatttctat attttcattc gatatttcaa actttttggg tagtttttat aaacctattt
    89041 atataactac acttgccggg gttgttagta ttgatgctgt tttagctctc ccatttgcaa
    89101 aattgagaaa tgaaaacaag ccatttttat tttctagttt aaaaatatta ggtgtagtta
    89161 ttaatgtatt ttgtaatatt cttttttatc tcatcattcc tagaaattct ctatatactt
    89221 tgttcccaaa tgtcgacctg ttatattttg tttttttatc taacttaata caaaatattt
    89281 ttgtattaat tttattgcta atttttacca agattccaaa attaattttt agtcccaagc
    89341 ttcttaaatc aattttaatt tactcattac ctcttctaat tgctggttta tcaggaacta
    89401 ctaatgaagc atttgataga atttttatta agtatttata tccaataaaa gacgaagcac
    89461 tttatcaact tggcatttat ggagcaaatg taaagttagc agttttttta gtattgttta
    89521 tacaaatgta taggtttgct gctgagcctt ttttctttaa attgcaaaat gaaagtaata
    89581 ataaggaaat atttgctaaa tccttcaagt attttttaat attttgtttt tttatcacaa
    89641 tacttataac ttttaatatc cctattctaa aatattttgt tggttcaaat tatcgtcata
    89701 atctttatat tgtccctatt ttacttatag caaattcact ttttggtata tactttaatc
    89761 tatcattttg gtttaaactc tccgataaaa ctttatacgg tcttaaatat gtaggtatag
    89821 gtgcttttac aacaataatt tcaaatgtta ttcttattcc tttaattggt attttcggtg
    89881 ctgcagtctc tagaataatt agctacacta ttatgacgtt actttgctat atagatggaa
    89941 aacgttcagg atatatttcc attgatagaa ataatttaaa gatattttta ttgtttttat
    90001 tatttatttc atttatctct acggttttat taatatatag caaaccttta ttttctttaa
    90061 tttttgcaaa tttattttta atattattta tatttttctt tttaaagaag gagaatataa
    90121 aaattttaaa tttacgaaaa tgctaaaagt taaaattgta aataaatcta gcttccctct
    90181 tcctggttat gctacatcac attcaagtgg gatcgattta tatgctaata ttagtagttc
    90241 aatcaaaatt ttatctcttc agagaatttt aataccaact ggaatttttg ttgaaattcc
    90301 tgacggatat gaagcgcaaa ttcgtccgcg gagcggatta gcccttaaac atggattaac
    90361 tgttttaaac acgcctggca caattgattc tgattatcgc ggcgaaattt caataatact
    90421 tataaattta ggtagtgatg aatttatact tgagcctggt ataaaaattg cccaaatggt
    90481 tttttccagg tatgaaagag tacactttat tgaagtaaat gagttgaccg aaacatcccg
    90541 tggagccggg ggattcgggc acaccgggat ttaattattt taaattcttc agtaatttag
    90601 aaggttacag ctgaacagaa ataaatttat taatgtcata ttttaaactg tgaaatatat
    90661 ttttaaatat tgtttgttct ttattttatg ctactgcatt acatttccta ttaaatcgca
    90721 gtctttagag gatttaaatt ctactaagag aaaaattgaa aatcaaatac agcagctaaa
    90781 tgttttaatt aatgaaaatg caaacctaaa gtccgaaact attaacaatc ttaatttagt
    90841 tagccaaaga ataaagttaa aaaattcatt aatcaaccaa ttagaattag aaatattata
    90901 cctcaataat aatattcttc aaagccagtc aaatatcgat agtttatcct ttttactgaa
    90961 tagcagaaag aaagaacttg agaaaattct tcaattttac caaaaatatc ataatagcta
    91021 caacatctta atatttattt tatcttccaa ctcttttaat cagtcgtttt taagaataaa
    91081 attttataat tctatcatta aatactattc aaataccata aaaagcatat ctacggatat
    91141 taataaaatt agtgaaataa aagtatctct cgaaaatcaa aaaaaggagc tttacctaaa
    91201 gcaacttcaa aagattgatg aaattaaaac tcttcgaaaa gacttagatt tatacaattc
    91261 aaaactaaaa aaattaaaat ctaatgagaa aaatttgaga aagcaaattg aaatagaaaa
    91321 aagacgaagt aatgaaataa tatctaaaat aaatgagctg attgaagaag aaaccagaaa
    91381 aaaaattatt tctaccaata aatcaaacga aaatgttaaa ttaagcaacg actttttaaa
    91441 aaattatgga aagttacccc acccggttgc aaatgctttt gttattaaca attttggaga
    91501 aaccaatcat cctgttttaa aaggagtaaa aatcaaaaat aatggtgtag attttgcatg
    91561 caaaactggc tccgaaattc gttcaatatt taatggagag gtaaaaaaag tatttaatct
    91621 acctatgaac ggaattgctg taattattcg ccatggtaac tatttatcgg tatactcgaa
    91681 tttaactaat gtatatgtaa aagttggtga taaggttatt actgagcagg ttataggtaa
    91741 aattgtttca aatgataatt ccggctcaat tttacatttt gaaatttgga atgaaaaaaa
    91801 tccagaaaat cctcttatgt ggatttacta acttttccta tttttgtaac cttttagaat
    91861 acatttagta taacgaataa aatattttgt atgcaaacta ttatagagtt aaatagtagt
    91921 ttagatagta tctcaaaagt tgaaaaattt atagacgaaa tttcagcgaa tgttaacatt
    91981 tcatctgaga tttatggtaa aattcttatt gctaccatcg aagcagttaa taatgcaata
    92041 gttcatggga ataaacttaa ttctaataaa attgttaggg ttaaaatcga gtccgaaggg
    92101 aataggttaa aagttatagt agaagacgag gggccaggat ttgatttcta taatttaccc
    92161 gatcctacaa cgccacagaa tatcgaaaat atctctggca gaggagtttt tttaatgaga
    92221 aaattgtctg atgaaataaa tttttttaat aatggtagtc tagtagagct aattttttac
    92281 ttgtgatggc aatttcattt caatcaaata taaactttaa cttaagcaat aaacgaaaag
    92341 ttaaaacctg gattaaaaat gttgttacta agtatggatt taaaattgga gatgtaacta
    92401 ttttatttac cgatgatgag tatataaaag aattaaatat taaatattta aatcatcact
    92461 attttaccga tatccttacc tttgactact cttcaggttt agttttaaat ggtgatattg
    92521 ttatttcaat cgatactgta aggtcaaata gtatcttata ctctacttca atcgacgatg
    92581 agctactccg tgttataata cacggatttt tacatctttt gggatttaat gataagtcta
    92641 gtgaagaaag tagaaaaatg agggagctag aggatggtgc tttaaatatt ttttattcga
    92701 atgactgatt tttttgatat tattgttatt ggaggcggac atgctggatg tgaagctgct
    92761 tctatcgcag caaaacttgg atcttctgta ttgcttattt ctctggattt aaccaagctg
    92821 gcccaaatga gctgtaaccc atctattggt ggaataggta agggacaaat agtaaaagaa
    92881 atcgatgttc ttggcggata taccgctaaa gtaacagatc ttaccaccat tcaattcagg
    92941 atgcttaaca aatccaaggg tcctgccatg tggagcccca gagcgcaatg cgatagaatg
    93001 ctattttcgt atgaatggcg aaaatattta gaatctattg ataacctcca tttattccaa
    93061 gacgaagttg ttgatattga ttttgatgtt aaaaaccaac ttgttagtgg tgttaccacc
    93121 caaagcggca ttaaatttaa atgtaatgtt cttatcctta caaatggaac ttttctaaaa
    93181 ggaaaaatcc acattggtag caataatttt tccggtggta gatttggtga aaaggcatcc
    93241 atcatgcttt cggacaaatt ggaagatatg ggattgcata ttggtagaat gaaaaccgga
    93301 acacctgtaa gaatagatgg gcgaagtgtt gatttttcaa aacttttaga acaaaaaccc
    93361 gatacttatg gcaagttctc tttagatcct aaatacactt ctaccttgca acaaaagtca
    93421 tgctatgtag cacacaccaa tagtgttgtt cacgatatta ttagagaagg tttcgataaa
    93481 tcgccactct tttctggaaa aattaagggg ataggaccaa ggtactgccc aagtattgag
    93541 gataaagtca gaacatttta tactaaagat tctcaccaac ttttcttaga acccgaggga
    93601 gtcaacactt atgaaatgta ccttaatgga ttttcttctt cactacctgt agatattcag
    93661 ctaaaggcac taaaaaatat cgagggtttt gaaaatgtat ctatacttcg acccggatat
    93721 gcaatagagt acgactattt tgatccaatg cagctaaatc atacattggc tcataaaagt
    93781 attaagaacc ttttttttgc aggacaggta aatggaacta caggctatga agaagctgca
    93841 ggacagggat tgcttgctgg gattaatgca cattacttta ttaatgatag ctcgaaacag
    93901 ttgatattgg gaagaaatga agcatatatt ggagttttga ttgacgatct tgttaccaaa
    93961 ggagttgatg agccttacag aatgtttact agtcgtgctg aatacagaat tctgattcga
    94021 caggataatg ttgatgaaag gttacttcct attgcgcaaa aactcaagct ggcttcaaat
    94081 gaagacatag ataggtttaa tgtaaaatct aaaaaattag aatcattcaa aaatttaatt
    94141 agcagcataa cggtttctcc tcaagatgta aatgattacc tggttaaagt taactcttca
    94201 cccattgaac ataaacgcaa gctaaacgat atattggctc gacccgatgt aaattttttc
    94261 gaactgttgg atagttgccc gaatttaatc ggaattttag gagacgttga taaaaatata
    94321 gccgaaaatt atattattaa aatcaaatat tccagctatg aggaaagaga gcaatttatg
    94381 gttgataaaa cccaaaagtt agaagcgctt aaaattccta ataacttcaa ttacgaatct
    94441 atacgctcct taagtactga gtcaagacaa aaattatcta aggttaaacc gaaaacaata
    94501 gggcaagcta agaatattcc cggtgtgagc cccagcgaca ttaatgttct gcttgtatat
    94561 ttgggaagat aattgttcca cgtggaacaa atgttttgaa atttgttcca cgtggaacaa
    94621 cttaaggcat tatgccgatt tgttttaggg atttcttctt aaatttttta tgttaaagaa
    94681 acacttgtgg agcgcccctg aaatgctgaa cacacaagcg ccctgcgata atcaagtagc
    94741 caactgcata aacttgatgt ctaaaacgaa ctggtcaaag tatttgaaat cagaaacata
    94801 aaaatttgta ataatgaata gatctggcat tgtgaaaggt gttgtgtaca atccgatact
    94861 acacgaatcg tttggggcat tggtctttat tgaaaatgga aaaatcctga aaatcgagag
    94921 ggatgggaat attaaggaac cgctaatact tcccggtttt atcgactccc acgtgcatat
    94981 tgaaagctca atgctcaccc ctgcgcaatt cgggagaacc gcagcagctc atggaaccgt
    95041 agccgctgtt gccgatcctc atgagattgc caatgttgca ggggtcgagg gcattaattt
    95101 tatgctgagt agcgcaatag agtcacccat aaaactattt tttggcgcac cgtcgtgcgt
    95161 accagcttca ccattcgatg aatgttttca gccttttagc ccaggtataa tcaaggagct
    95221 tatcgataga agcgatattt tctttttggg tgagatgatg aatttccctg gtgttatcgg
    95281 tggtaacgca gaggttatgg aaaaaatcaa cattgctaaa agcgcaggta aacccatcga
    95341 tggacatgcg ccctcgttgg acaaaaacga cctaaaaaaa tacatttcag ctggaatttc
    95401 taccgatcat gagagttttt ctctcgaaga ggcacttgac aagctatcgt ttggaatgaa
    95461 ggtaattatc cgcgaaggta gtgccgctaa aaattttgac gccctgcatt cgctaattcc
    95521 tcggtttcat gataacctta tgttttgcac cgacgactgt catccggacg atcttatagt
    95581 tggacatata aacaagcttg taaaaaaagc catcaacgcg gggtataata tcttcgacat
    95641 tctccaggtt tcttctgtta atcctgtaat gcattacaac ttaaatgtgg gattacttca
    95701 gcccggagat cctgccgatt ttattgtggt cgatggttta agtagcctta acattatttc
    95761 tacatacata aacggtgatg acgtgcttaa ggagccaagg ccgacaacaa atgtcaaaat
    95821 tccaacttac acttttccta attcgttcga ttcaaacctg ctcgttaaaa agctggacac
    95881 taagcgtgtt aaggttattg atgtagttaa agatgagctt ataaccaact ggcatgttga
    95941 gaactctaac accgatctgc tggaagcaaa cccaaaggaa gatctcttga agattgctgt
    96001 tgtaagccgt tataagaaaa atgaagcatc tgttggtcta atcaggggtt ttggtatcaa
    96061 caaaggtgcc attgcagcaa gcattgccca tgatagccat catatcattg ccattggttg
    96121 tgacgataac tccatcacac aaagtataaa ttatattgtt aagtgcggag gtggaatttg
    96181 ctatttcgat ggcgatagta tttatggtct cccccttccg gctttcggat taatgactaa
    96241 tgaatcagcc gaggtggctg gaaaagccta ccacgaatta acccaacgtg ttattgctga
    96301 tggctgtaaa ctgcaagctc cctttatgac catgtcgttt atgtcgcttt cggttatacc
    96361 ccatcttaaa attaccccca cctccctgtt cgatgttgat aaatttagct ttaccaatat
    96421 atgcctatag cttattaaat tagcatttat aactttatct actagatgaa tgacatttta
    96481 tcgaatatcg ttactaacct cccggactcg ccgggtgttt accaattttt cgatataaat
    96541 ggaattctaa tttacattgg gaaagcaaaa aaccttaaaa aacgggtttc atcctatttt
    96601 catcaatcgg cttacgaatc ggcaaaactt agagttctcg ttagcaaaat atgtgatatc
    96661 aagttcattg ttgttgattc tgagtcagat gcactcctat tggagaatat tctgattaag
    96721 aaacacaagc cccggtacaa tgttttgctt aaagacgata agacctatcc ctggattgtt
    96781 attaagaacg aaccatttcc ccgtgtttat aaaacccgca agtatattga tgatggaagt
    96841 tcttactttg gcccatacac atcgggaagg ctcgtgtttg aactgctcga tctaattaga
    96901 aagctttacc ctgtacgtac ctgctcgctc aaccttacta ctgctaacat acttgcaaaa
    96961 aaatttaaac cttgccttga ataccatatt gggaactgtc ttgctccttg catcggcatg
    97021 cagagccatc aaagttatct tcaaaatatt gatagtataa aacgaatttt acagggcaat
    97081 ctttccgaaa taaagaatca tttaaaggct gaaatggtta aatattcaaa tcagtataag
    97141 tacgaagaag cacagctgat aaaggaaaag atcgacatat taacgaattt tcaaagcaaa
    97201 accactattg ttaacccaaa atataaggat atcgaagttt ttactatact taccaggcat
    97261 aacattgtag gagtaaattt tattagaata aataaaggcc aggttgttca atcctataac
    97321 ctacatctga aatccttcgt aaacgaatcg cttcccgaat tattttcttc tgcaatagcc
    97381 gaaatcaggc aacgtctcaa ggctaattct actagaatac ttgtgaatat catgcctgaa
    97441 tttactatta atggggtttc cttttcagtc ccacagcgtg gagatagaaa aaaattcgtt
    97501 gaattaagct taagaaattt agagatattt cttgatgacc tctataaaaa tgaagcagct
    97561 aaaagccctg tcttagccgt tgactcgatt ttgaccaaaa ttaaagacga cctaaacctg
    97621 agcgatttgc ccgtccacat ggagtgtttc gataattcga atattcaggg aagcaatccg
    97681 gttgcatcat gtgtggtttt taaaaatgct aagccttcaa aaaaggatta ccgtcactat
    97741 aacataaaaa cagtccaagg ccccgacgat tttgcctcta tgaaggaggt tgtttacaga
    97801 agatattcac gaatgctttc tgatggcgaa acccttcccc aacttattgt tatagatggg
    97861 ggtaaaggac agcttaacgc cgccatggaa agtattgttg agctcggaat tcaaaatagg
    97921 gtacatttaa ttggaatcgc aaaaagactt gaggagattt ttgttcccgg ggatcctgtt
    97981 ccggtgtacc ttgacaagcg ctcagaaact cttcggatta ttcaacaaat aagaaatgaa
    98041 gcacaccgtt tcggaataaa attccaccgt aacaagcgaa gcaattcagc atttgaatct
    98101 agtctcgagc aaattgaagg aattggaccc aaaaccatcc agctactctt tacccgttac
    98161 ctctctcttg atggaattag gaatgcgcca gaatctgagc ttattgactt aatcgggact
    98221 aagcgaacga accttatcaa gaattacttc aaaaaagttt agtatttttg gggattaagt
    98281 attattaaca accatatacc atatactcct tactgccata tatttgtaat ggatagtttg
    98341 aatttacgca ttaatttata cgacaaccta aaacaagcgt ttttttctcc tgatacgctt
    98401 aacggtgggt gtacaattag tccatcagcc gacacagcct tagcctcgtc aacaaagcta
    98461 aacaatgttg tttcttcatt gagtggcgat gcaaatgtga tggtcgagcc aattctccgc
    98521 aaaactaaca acattccaaa ttcctacatt attccatttt tagcagggct agtggtttta
    98581 gtagtcatcg tgttgcgtta ccgaaagtat ctggcctcca tagttgaatc tgctttatat
    98641 tcattttttg cggagaaatt tgttttggaa acaaatgttc cattccgccg aacttttgcg
    98701 ctgctcgatg ctctgtctgt ggtttcgttt agctgctttt ctttggttgc attgaaactg
    98761 cttaacactg aattttttta tgaatcaaaa cctataattc ttattgccat tgctatagcc
    98821 tctttttctc tattcagatt attcattagt atatataact acttgataaa tagcatatta
    98881 tccaacaatc cgattttttc tttatttcaa tataactact ttattttcat aagaatagta
    98941 ggatttatta ttcttccaat ttcattcgtt gctttttatg cccctacttc ggtaggactt
    99001 tacctcctct attttatgtt tggtattcta ctagttaccc tattctatag gctataccgg
    99061 cttactttaa tatttgtaaa aaatcgtatt tcaattttgt atttcatttt atacctttgt
    99121 ggcctggaag ttgtcccaat tcttatcgtt attaaggagg tacaacgcat ttaattcgtg
    99181 ttttaatatt ataaggcttt gaaaatcagc agaattgtcg tagcccagca agaacctgtt
    99241 aacgagaaat ctccatatct tgatatttct aataggtatg gagttaaaat cgactttcgt
    99301 ccatttatac atgttgaagg aatctcagct aaagattttc gagctcaaaa aattaatatt
    99361 tccgagcaca ctgcagtaat ttttaccagt aaaaccgcta tagaccatta ctttaggatg
    99421 tgtgaagagt taagagtcac cattccggaa acaatgaagt acttttgcat tacagaatcg
    99481 gttgcgcttt acctgcaaaa atatatagtg tatagaaaac gaaaggtgtt ttttggaaac
    99541 aatacttttc ccgatctgct cgaaataatt caaaagcata aagaggaata ttttctggta
    99601 cccctttcgg atattcataa gcccgaaatt cccaaggcgc tcgagaaagc aaaattaaaa
    99661 ttccggaaag gggtatttta taaaactgtt agtagcgatt tatccgacct aaaccctttt
    99721 ccttacgata tggttgtttt ttatagccca agcgctgtta aagccctatt tgataatttt
    99781 cctcagttca agcagaatga tgttgtcatc ggtggctttg gaaaaaatac ggcaaaggct
    99841 atcaaggacg ccggtttaag gctggacatt agcgcgccta ccactacctg cactagtatg
    99901 accctggcta tagacaattt tttaaaaaat ttaagcaaga aatctaagta gttaaggcac
    99961 ttcctactta atttgtatga aatatttctt gcgaaagaac aatcgcttaa cgctacagca
   100021 ccaggttgac cttcttttta gccatggtta tgggtatttt atttacccca tcagggcaaa
   100081 agttctgcta tacgatcgga atgttgaaga agacacatgg tttaaagtaa tggttgttgt
   100141 accaaagagg catgtcaaaa aatcaagcag caggaatctt ataaaacgaa gaataaagga
   100201 atcatttcgg gtggcaatac attcattaaa aggttcttgt ccttcaaaca aaattatctt
   100261 aatagctatt tctttcgttt caggcgatat taagccctac gatagaataa aggttagcat
   100321 tgaaaaattg atgaatgaaa tcgaaaacga catagaaaag ttaaaatcca taaaggatta
   100381 aacatttttc aaggcgaatg ctttaatagt tgtttattaa gaattatttt gtgttgaatt
   100441 tcatgatatg aacaagaata aaatccgtga tgttttaatt cttttacctt tagggttaat
   100501 taagttttac caacttttta tttcacccca catccccaat agctgccgtt atactccaac
   100561 ctgctcggtt tattcggtag aagcgctaaa aaagcatggc ctttttaaag gaagctggct
   100621 ttccataaag agaattttaa ggtgtaatcc gtggggcggt catgggtatg accccgttcc
   100681 ttaatctacc gcttttttaa aactatccta aatgtagttc caactccagg ctctgaattc
   100741 aggacaaaaa ttttcccccc gtggtgctgt tcaataatac gcttagaaag ggttaaccct
   100801 agtccccagc ctctatcttt tgtggtgaag cctgcccgaa atatttcctc aaattttccc
   100861 ttctgtattc cccttcccgt atccgaaata tcaataaata caacctgggt attgtccgta
   100921 actttaaagg ttatctcacc ctttcccatc atggcatctg cagaattctt aacaatattc
   100981 tctataaccc attcaatcag aatgccattc atgcggatgt tcaatttttc ctgaacatta
   101041 tattttttta caacagtcac attttttaat actctcgctc ccagatagtc aatagtgcta
   101101 ttaagcagct ctattaggtt tgcttcctga agtattggag ttgagccaat tttagagaat
   101161 cgcccagcta ttttttccaa acgcacaata tctttttcaa gttccattat gatagggtca
   101221 tttggtattt tttcttccag aagagctaaa cagcccatta acgaagaggt aggggtgcca
   101281 agctgatggg ccgtttcctt tgccatccca taccaaatgc tgttctcgtg tgctttgcgc
   101341 gattgactat aggcgtaata tgaaatgaga aaaaagagaa gaattatgac gatttggatg
   101401 taggggaaaa attctagcct tcggagggtg gtgctatccc ggtagtacac atagtttttc
   101461 gtgctatcgg atagcataat ttcaatgggc tcatgctctg atgtcatttt tctaagtagc
   101521 ctataaaccg ctactgagtc ttttatttgc ctctcggaaa tgttcaggtg cgaaattatt
   101581 cttccgcttt cgtctgtaag caaaacgggc aaggttctat tattttgtat aacctctgaa
   101641 acaaaggtta aatcttggca tgaatcctgg gtagtggaga gaaaccttac gcctttggcc
   101701 cataaatcta ttttggcatg ctcctcttcc gataaatttt ttacaagctt attgctgacc
   101761 agtagcgaaa aaatcacgat aaaaccggca aatataataa gcgcaaaatt ttttaaggta
   101821 ttacccgaaa tactcataaa cattatggct atttagtttt agttgtatca tattcatccc
   101881 actcaacttg aaatttttga gatgttggtt gttgctgtat agcgtgttgc ttctttaaag
   101941 aatcttcaaa ggtttgctta aattcgttgc ttatgatttc ttttaacgat tgaccctctg
   102001 tattaattgt gtttttaatg gcttcgtagc tgcttttttt gtcaagaaaa acctcatagc
   102061 ccttactatt acccttcagg gttaagtata ggtttattgc gccgctgcta gttttaggct
   102121 ggttatagtc tgctgaataa tttaagaacc tctgggccag taagctggaa aagttaatac
   102181 gagcatggta ttcataatcg tttgtcagga agtgctttcc cgaaaggttt aaccccataa
   102241 cgtttgaatt taaatccatt gaggggatgt aaatacagcc attcgaaatc aaaatgttat
   102301 tgtgaatagt ggtaaaatct atcgatttta cctggtcgag tttaagccat tttgaaagct
   102361 tttttatgtt attcatgcct ctcaatttgc catcgtttat agtaatctcc gaattcattt
   102421 ttgccctgtc ccacctaatg ttgttagcac tatccagctc aaaatcaaag ttgcaatgcc
   102481 ccgagagatg cccttctatg ttgttatggg taatgtactt ttggttccag ctgttataat
   102541 gtctgaaaag ttcggtaatg tctataccct ccatcgatgc gtttatagag tacttatcac
   102601 caacgaccat taaatcacca tcaattttcc cttcgaaacc cttagcattt agccctttaa
   102661 catatatact atccccgact atattcatta ctcccgttac attttcaaag gcatagttgg
   102721 tatatataag ttttttagtg ttgatgcttg tttcaactgt cgtataaacc ctttttttac
   102781 cacctttatt tacggtagaa actgatttta ttaagctatc taagtttaaa tagctagaat
   102841 ttaaatatat tgatggagaa aaatttaacg gattatttaa atagtaaatg gcattattct
   102901 gacacaatgt aaaatcaatt tttgagctat ccaaatagca cgtagcgcta ataattgcct
   102961 tatcgcgaat tgtcgctttt cccgaaatgt tagacaaatt tttgaatgca gaaatcgaat
   103021 caaggctgaa gctaatctcg ctggctacat catgcaacaa aaatccaaaa tgtttacttt
   103081 gtagcccgtt accctttctt agctctagta aagccttaaa ggtaccgttg ccttcaagcc
   103141 cggttagttt attagtaaat gaaggttcat tttttcccag ctttaataac ccctcggcga
   103201 gtattattat tcgatttgtt ccataggttc tacccgaaaa agcagcttcg atattctcgt
   103261 aacgaaccac tgcatttgat acttgcccgg aatgtgtttg caggttgttg gtaaagtaac
   103321 ttaagccgtt aatttcttta atctcaataa ttttaccttt agcaataata ctaacattct
   103381 gggcatcgta gtctatccat aggccggtta aatttttttt acctccatta tacgacagct
   103441 tgcccgacat tgcgacctta cccgttatgt cggcaggcaa tccctttata ttggcaactt
   103501 ggattaaccg gttaatgttt gtcctgttca gtttaaaacc caggccaagg gtgctgtttt
   103561 ccaggcgata caatccctga agttctaatc tgctaccact taggctaatt tttacatttc
   103621 gcaggaagac gcttttccca tcatagctag ctattgcatt tacctgtgtt tgttcattta
   103681 gcataaactt acttcctaaa tttatttcct taagctggca atcaaggttt aacgttaaaa
   103741 ttttttttgc tcttatgtta agagtaagcc tatctattcc gattaatgat ttttgcctgc
   103801 taagtgtatc ctctattaat gctttaaagc tcgaaagagt aaacctattg atgaagaact
   103861 cgttttggag aagggctgct tgtccgttag caggatttat atcgttcttt tttagtaggt
   103921 ttgtttttag atttagcctt ccattttctg ctttacatga attaatttcg aaattcccca
   103981 gcagtagcct tgctgcatta atttttaaat ccaagttttg cgccgacatg agcgtatcat
   104041 tattcccatt ttctggtagt gtaagtatga agtctgaaaa agaaaaggtt gcaaatggaa
   104101 aatttttaaa cgtatcaaac ttaatactac tgtagtatat gcttactacc tcatattcat
   104161 taaactgttt taataacaaa tcaatcaatt tgctctgatt tataaataca taagttgtaa
   104221 gcaatattag tgctaaaatt ataccactta aaaagtaaag ggtaaaaagt aatcctttgc
   104281 gagcaaatcg gcctaaaaaa ttaaacagcg gtttcattct tgctatgttt tcagttttcc
   104341 ggttacaaac acttccgctc ttaaccttat tttaaaaggc gttgagcgga atgaacatcc
   104401 atattttggt ttcacccagc cagcaggtaa cgccaggtag tttgaccatt gttaagcctg
   104461 ctacccaata ttgatagcgg tatggcttta gtatagaaaa tatgtatttg tttataattg
   104521 aacggtgact ctagcgagat gttttataat ctaatatttt gacataccac gctaatagca
   104581 accggcatat tataaatact tcctaatcct atacattttg gcaaaatccc taaatgttaa
   104641 atagatgttg ggagatgcaa gcaaagggaa aggctttagc accagtttac acgcccatta
   104701 aaaatccaca aatttatttt tttgctggta aatacccata ttttttctat acgactagta
   104761 attaagtata aataccttta agataacgaa taagcacatt ccgataaatt aatagtttag
   104821 gtagtggtat atttttcggt cgtctgaaac tgttaaaagg ttataagaaa ttattatgtg
   104881 ctttgtagat cgattaaaat atataaattt acctgtttct tttaggtgaa tatcaacgtg
   104941 aagcatttgc caggaaagca aaattaagat ttatgaaatt tcggatagtt tctctctgtt
   105001 tattatttgc cttcagggta ggcatatcac aaaatatcga ttccctgctg ctaaagctgt
   105061 accaggagtc aaatgatagg tcgaggatcg aaatcttact aaacatatca tctttttacg
   105121 atcagcatga cagaaatttc atacaggccg aaaattatct atacgaggca ttacagatag
   105181 cccagacagc tggagacaat actcttttag ccaacaccta taacgagctg ggcaagtttt
   105241 atagaaatca gtcaaaatat ccggaagcac tcaaatacca tcatcttgct gactctttgg
   105301 ccgttaagat gggtgattgt attcttcagg cgacaacatt aaacaacata ggtgttgtct
   105361 atcgaagact cgacaaccat gcccaagcat ctgaatacca tctaagggca cttaatattg
   105421 ccgagggatg taaggataca ttcaatattt ccgttgcctg taatagctta ggaaatattt
   105481 tttcacttaa tggacgttac cagcaggcac tttcatattt taacagggcg ctggccctat
   105541 ctattagcca gaataaccta cttggccaag cgatgaactt caacaatata ggtgaagttt
   105601 atgagtttat gggagattat agaaatgcca agtactacta caccaagtcg ttggatatca
   105661 atttcaagtt aaaaaatcta aagggaatct caatcaacta taatgcgctt ggaaaaatag
   105721 aaatgtttaa tggtaatccc gaaaatgcgt acagactttt ccaaaacgct cttgacatag
   105781 atagaaagct aggtgataaa aagtttcttg ccgatagcta tgtaaacctg gctaaagcct
   105841 gtctggccct aaaaaaaatt gacaaggcaa aaatctacct ggacagcaca attaccatag
   105901 cccaacaaat aggaagccct tggcatctcc agtctgccta tgaagcatac tcggattact
   105961 ttgttcaaaa atcagattat ttatcagcgt tacttagcta taagaacgct tcacggttca
   106021 aagacagcct tttaaacgag aaaaatgcac gacatctttc aacaatacag acaatttacg
   106081 aaaccgaaaa aaaggaaaag gaaattagct acttgctgca agaacaggca ctaaagcaaa
   106141 aagaacttga aaagaataac gcacagaaaa atgctctcat tattggactt gtactaagct
   106201 ctttagttgt tattgtttct ctgtttgcat ggagaacaaa ggtaaaatca aacattgttc
   106261 tatccgatca aattaaggag attgaggata aaaatcggct ccttgaaatt caaaaggaag
   106321 aaattgaaca acaaaaaatt aaaatagaaa gcaaccaggc ctttatcgag caaaaaaata
   106381 aaaatcttga agaggcgcat aagattattg agagctacat cgaaaatatt acagatagca
   106441 ttaggtatgc agaaaaaata catgagtcaa ttcaacccag cttgagcgat atgctatcca
   106501 ttttcttaga tgcgtttatt tacagcaagc ctaaagatat tgttagtgga gatttttgct
   106561 ggttaacccg aagggataac ctggtttttc tggctctggc cgactgcact ggacatggcg
   106621 tcccgggtgc ttttatgagc acaataggta ttaacattct gaatcaagcc attaacgaac
   106681 aacaccttta ctctcctgat aaaatactaa ctttcataaa tcgtactctc attaatagat
   106741 taaataagtc aacaggagag caggttctga aaaacagcat ggacatagct gtttgtactt
   106801 ttgataccga atcgctttta ctgagctatt ctggagcgct tattcctatt tttatccaaa
   106861 aggggaattc cgttgaagaa gtaaaacccg actctattac ccttggcaca aactttagcc
   106921 actcagcatc gggcttttca cttcaaaaca ttcagcttaa tcatggggac tgggtttact
   106981 tatcctccga tggttttttt gaccaacttg gtggcgaatc acgtaaaaaa ttcacgcgaa
   107041 agcaattcat gcagatagtt gcggatagtc ctaatatgaa gggtaatgtt aaggtagata
   107101 taatcgaaaa gaaatttttg cagtggatga atagaacccc gcaactcgac gatgtgcttg
   107161 tttggggatt tcaggtatag agcccaaata ttatttaaga aaattttcgg actgttgaaa
   107221 atccccgata caacatccct acaacttaaa atttccaata cccacaccat caacatgaac
   107281 gaaaacagga gcatcgaacg caaacgtttt tgcatcagcc tttttgtatc gcttattttc
   107341 attgtagcgc tttggtgcat taaaatcttt gaaatagttg agggtatttc actctatagg
   107401 ctgggtgttt ttcctagaca aataagcgga ctgcctggaa taattttttc accattaatc
   107461 cattcagatt tcaaccatct gataagtaat tcgttatcgc ttttattctt acttattgga
   107521 ttactatatt tttatagaga cctatcatat aaggtaatat tgtttatttg gattacaagt
   107581 gggctaatgg tttgggttgg agccagggac agctaccaca ttggggctag cggtctcatt
   107641 tatggattgg cctcgtttct tttctttagt ggtgtggtaa ggcatgatca aagacttatg
   107701 gcaatttcgt tgattgtggt gtttttttat ggtgggttaa tatggggaat tcttccggtt
   107761 tatccagcaa tttcgtggga gtatcatctt tttggcggta ttagcggtct aattgctgca
   107821 atattatttc gaaatcaggg accacctaga aaagtctgga gctgggaaca ggaagaggat
   107881 gaaaatacac cagaaaattt gtagtatttt ttctgtacaa aaattggttt ttggttacaa
   107941 atacatttgt tcgaatgctt taaacatcgg ggttaagaaa attgaacagc cattttagct
   108001 tctacaaact tgcgttaaaa accggatgtt cctgcgggtt tgtgcaagtt gacttgcagg
   108061 agtgtttcct gtcttgtcat caacctaacg ctttatagaa tattcaacat ggatgaccct
   108121 tccctaattt cccaataatt ccaaacgaga gaatcccact ttttaaatac ttttgcctaa
   108181 ccagaaaatt atggaatgga atcccatatt ataattaatc aactccttgc ctttatagtt
   108241 ttagctgtaa ttggagcaat tgcctcgtgg aggaaaatta ttaatgacga ggggaaagat
   108301 ttcctatcac gattaattat tgatgttact cttcccttat tgattgtttc cacctttctc
   108361 aaactcgaaa gcaacccccg acttcttgta aacggtttat ggctatttgt tctgactttt
   108421 gtaaatattg gtgtcctgta tatggcagga acgttgtctg ccagagcaca aaagttatct
   108481 ccatctgact caaccgtgca tgttcttcat accatgtttg gaaatattgt ttttctgggt
   108541 tttcctttat ttgatgccct ctttcctaac ggggtaggag ttttttatgc ttccgtgtac
   108601 cagctggctt ccaactttat tacctatacc tatggaacct ataagctaag tgcgggagtt
   108661 caaaaatcca aattgggtaa tttttttaat ataaacacaa ttgccctgct gctcggcatc
   108721 ctgctcatgg tgttaaaggt taaactacct gcttttatcc tagagccaat ttcgagtctt
   108781 ggcaaatgta ccagcccgct ctccatggtt tatattggag cgctgctggc cggcatgaac
   108841 ttgaaatcaa cttttaaaac tacaagtatt tatgttctta caatcaacaa gttaataatt
   108901 gtacctttac tactaggttt tatatactct tttctcagaa ggtcgtttaa tttaaaccta
   108961 tcaatagagg catttgtcgt tttggttatg caggcagcta tgccttgcca gacgattgtt
   109021 gtagtaatgt ctagacgtta cggcggaagc cacgtactag caacagggaa cttatttatc
   109081 tctaccatct ttagcatcat ctcactacct ttggtttacc attttttggt aagtatttat
   109141 tcttagttaa atatattatt aaattataaa acgataaggt gcatataatg aatcaaatat
   109201 aaaaaatcaa aacgatatac atagtttttg tgcgtagatt cacaaatttt caaatggaat
   109261 aatttttaac tattattacc atgagagtag ttattcagcg tgttaaaagt gctaaggtgg
   109321 aagtaaacaa tcatgaagtt ggctgcatac acaagggctt attggtcctt tgtggttttg
   109381 aagtggagga atcggaagag gatttacaat ggatggcctc aaaaatttgc aagctgcgaa
   109441 tatttgatga tgaaactgaa accatgaacc ttagcattaa ggatatatcc ggagaaatac
   109501 tagtggtgag tcagtttacc cttcacgcca gcactaaaaa gggttgccga ccatcatatg
   109561 tgcgcgccgc tccatcttcg ttggccatat tgttttacaa cagattctta aatttattgg
   109621 agatcgagct gggctctaaa atcgctgctg gggtttttgg aaaaatgatg gaggtttcat
   109681 tagtaaacag tggccctgta actatagtga tagattctaa aaataaagaa ttttgaaaat
   109741 gacattcgaa gaggcacaga aggaagttga ctcctggatt agggatcacg gcgtgaggta
   109801 ctttagcgag ctgacaaaca ccgtgctttt gatggaagaa gtcggcgagc tggcaaggat
   109861 tattgcccgt aaatttggcg agcagtcttt taaagataac gaaagcgccg atggcttatc
   109921 cgacgaaatg gcggatgttc tttttgtttt aatttgcctg gccaatcaaa caggggtatc
   109981 cctttctgaa gcatttgaga aaaatattga aaaaaaaaca caaagagact cccagaggca
   110041 caaaaataac cccaagttat tttaactttg tagcataacc gaacttttaa tattttatac
   110101 agtatggatt tttttgttga agataaattt aaccttgagg ttaatgggga agaagtcggg
   110161 cgccatttgg cacgaattaa ggatctcgca ctttcccggc ttggtgatac cgataagttg
   110221 aaggcttgtt ttggctcgat tgacctaaca accttaaaca caacagacac tgctgaaaga
   110281 gggaaacaat ttgctgaaaa agttaacggc ttcactgttg attttcctga aatgccaagc
   110341 gtggctgcaa tttgcgtata tccaacacta gtatcatccg taaaggagaa cctaaaagct
   110401 aaaggggtta agattgcctc cgttgccgca ggatttcctt cgtcgcaaac ctttttggag
   110461 attaaacttt ctgaatgtag gatggcaatt ggccaggggg ctgacgagat tgatgtggtt
   110521 attccggtgg gatcttttct acagggagat ctgtatagcg ttttcagcga ggtaaaacaa
   110581 ataaaggaaa ctgctggcga tattcatgtt aaggtaatac tggaaacggg agcccttcct
   110641 tcgctgaaag aaataaagct ggcttctttt ttagctatgg aggctggagc cgatttcata
   110701 aaaacatcta caggaaagct tgaacccgca gctacacccg aggccgtttt tgttatgtgt
   110761 accgcaatta aagagtactt tgaaaaaaca aaccgtaaag ttggcattaa gcctgcaggc
   110821 ggcgttgtaa cttcttccga agccctggtt tactatacca tcgttgagca ggttttgggt
   110881 tcgtcttggc tatcccccga cctgttcagg ctgggagcaa gcagactggc aaacaacctt
   110941 ctttcagata tttatggtcg tgctgtaagc tacttttaag tttcagaaca tacagaacaa
   111001 gtttctgtta tataattgaa gaaagcgctt atttagccaa aggttgcttt atccctgggc
   111061 taggctcctg aaaacctctt tttaccataa gggcgacgga tatacgccat taagcgttag
   111121 cttcctgagc tgatcaacca catgaatttt atcgccagga taggtaaccc caaaccattc
   111181 ggaagtagta gaaagaacct tacatgttgc caatccatcc tgaatggcac ggttgacaac
   111241 tgttggtata tatatttccg attttttgtg atcggggatc ccgtttaaaa actctttaag
   111301 caatgtttct gtaagggtaa aaaagtcctg agtaaacccc cagaaattca tcgataccag
   111361 catgctgccg tctagctcga tgcggttacc cgagtcgtct atattaaata ttttcccatc
   111421 aaccctttga atcctggtaa actcaacaat actcttaaga aatgatgcgt tgtcaacttc
   111481 acaagcgccc ctagacacag acccttgttc agagagtgta ttgttgagcc tgaatcccac
   111541 cataaagtat tggccgtttt taagattgct tcttgagagc tcgtttgcca gagttttaaa
   111601 tgtatcgtaa ccataaaaat catcagcatt tataacggca aaaggctcgt taaccatttt
   111661 acgggctacc caaacagcat gagctgtacc ccagggtttt tccctttccg gaggacaggt
   111721 aatgttatcg ggaagcatgt taagctcctg aaagactata tcaaaattaa catcccggct
   111781 aaacctgttt ccaaaaattt tcataaaatc ttcctcgata ctgcggcgga taacaaaaat
   111841 aacttttcca aaaccagccc gaatggcatc gtaaaccgag taatcaatta tagtttcgcc
   111901 gtatggcccc agaccatcaa cctgctttaa accaccatat cggctgccca ttccggcagc
   111961 caggataatt agcgttggct tcatcatggt atgctttaca aacttaaatt tttagggttg
   112021 acaaaagtag catttatggc acagctatgg taaaccaacc attcgaattt tgatattttt
   112081 tgcaatataa tttgataata ataacgatat agtccatatg tatagccaca tagaatcagc
   112141 aattaaacta aaagtttaaa cattgaattt ctttctatac aaatagctta aaggtattaa
   112201 cagttaaaat acctactttt gcagaaacaa gggcttaatg atgagacgtt cggatataga
   112261 agttatggct cctgcgggaa actttgaatc gctgatggct gcaattcaag ggggtgccga
   112321 tgcagtttac ttcggagtcg gtaaattaaa tatgcgggct aaatccagcg tcaactttct
   112381 tgaagacgac ctttacgaaa ttgttcgcat ttgctcagca agcaatgtta aaacctacct
   112441 gaccgtaaat accgtgcttt atgatgagga catagacgac atgcaccggt taattgacac
   112501 tgccaaaaag gccagggttt ccgcggtaat tgtttcagac caggccgcaa tggagtatgc
   112561 ccgtcaggta ggagtcgaag ttcatctttc aacgcagcta aatataagca atacccactc
   112621 gttaaggttt tactcgcagt gggccgatgt ggtagtgctt gccagggagc tcaaccttca
   112681 gcaggtcaaa ttaatttcgg aaggaattca taagcataac attaatggtc cctcggggaa
   112741 caaggttagg cttgaacttt ttgttcatgg agcactttgt atggccatct ccgggaagtg
   112801 ctaccttagc ctccaccaaa agaattattc tgccaatcga ggcgaatgcc tgcaaatttg
   112861 caggagaagc tatacggtaa ccgataacga aaccggctcg cagctcgaaa tagacaacga
   112921 atacatcatg tcacctaaag acctctgcac tatttccttt ctcgatcaaa ttcttgatgc
   112981 gggcattagg gtgcttaaaa ttgaaggacg ggctaggggt cccgagtatg taaaaaccgt
   113041 aacatcctgc tacgctgagg cagttgaagc aattgtttca ggccaatata cacccgacaa
   113101 ggttgaaggt tggactaaac ggcttacatc ggtattcaat aggggctttt gggatggtta
   113161 ctaccttggg cgcaggttgg gggagtggag caattcctat ggaaatatcg caacggagcg
   113221 caaacagtat gtgggaaaaa ttagcaacta cttttccgac atgggagttg ccgagctaaa
   113281 cgttgaggca acggagctat accttaatga taaggtttta ataatagggc ctactaccgg
   113341 cgttattgaa acactggtat ccgaaatccg tgttgataac cagcaggcca gctttgctcc
   113401 aaagggactt ccgtgctcta ttcctgtccc atcaaaggtt cgtcgctccg ataagcttta
   113461 caggattatt tattctaagc cttgattgca gcctatacca cccaagcgtt aacttaggaa
   113521 tgggtagaat gttttaagtg atattttcag aaacatcaat acatgattga taaacaaaat
   113581 tataaatgct caagttgaaa tttttatttt agatgatggt ttaaagattt ttacaaggat
   113641 ttcggcacat tgcttaaatc ggtgattgta ataaccacaa aagatctttt tgaattgtta
   113701 atcattgaat ttatttaaat atatcattaa gtaacttaat ataaattaac tatggttaag
   113761 aattttagcg agttgtgcaa taacattttt gatgaatgca caaaatacta ccacactatg
   113821 gataatgtgg atgctaaccc aaacgttcct tataaaagta acaccatcga gcactccctc
   113881 ttcattaaga attggattga tgcagttcag tggcacctgg aagatttgat tcgcgacccg
   113941 aacatcaatc ccgaaaaagc gcttctgcta aaacgaagaa tcgacaagtc aaatcaagat
   114001 agaaccgatt tggttgagca aattgacagc tactttttag aatactttaa ggatgttcgc
   114061 ccccaaaatg gggctacaat caataccgaa agcccggcat gggcagttga caggctttcc
   114121 attctagccc taaaaattta ccacatggaa caggaggtta gcagaactaa tgcttcagct
   114181 gatcatgtgg ctacttgccg acaaaaatta actattttac tggagcaacg aaaagatctc
   114241 tccatggcaa tagatcagtt gttggacgac attgccaatg gcagaaagta tatgaaggta
   114301 tacaggcaaa tgaaaatgta taacgatccc tctctgaacc ccattcttta tggcaagaat
   114361 gaaaccaacc cttaaggaga acaaaccgaa gaaacttttg gtaatccgcc tcagcgccat
   114421 tggtgatgtt gccatgaccg ttcctgttct ctggtcggta gtaaagcaca accctgacgt
   114481 tcgcgttact tttgtatcgc agagctttgc tcgcgacata gttagccaaa ttcctggaat
   114541 tcaattttat gaagccgaca ccaagaatcg ccataagggt ttttggggga tttttaaact
   114601 tttcagggag cttaaaaatc ttgataggtt tgatgctgta gccgatttgc acaatgtaat
   114661 tagaagccgg gttattggcg ttttgtttag gattggtgga atacgctatg aggtaataga
   114721 caagggaaga aaggagaaaa aacaactatg tagcgtaaag aataaaaaat tggtagagtt
   114781 gaagcctact accgaacgct atgcagatgt ttttcgtcac ttgaacctgg tcgttagtag
   114841 cgatttcgat tacttatttg acgaggcccg gttgcctgat gatattagtg catacctggg
   114901 gtataaaagt ggcacctgga taggtgtagc tccttttgca aagcataaag ggaagatata
   114961 tccgctcaac ctgatggaag ccgttgtgtc gagattggca gcaaataaga gttataagat
   115021 agtgcttatt ggtggtagaa attttgaaaa ggagttgctg gaggagtggg caaaaaaata
   115081 cacaaataca ttttcagttg ccggtaaata cagtatttta aacgagctta aaattatttc
   115141 taatttggac gtgcttatat ccatggactc tgccaatatg caccttgctt cgcttgttaa
   115201 cactccggtt gtgagcattt ggggcgccac ccatcgtttt gcgggatttt acgggtggaa
   115261 gcagaacgcc gataacaccg tagaagttga gttgtcctgt cgcccttgct cggtgtatgg
   115321 gaacaagcct tgtttccgta aggactacgc atgtttaaac cgaatcaccc ccgaaatgat
   115381 tattagtaag gtaaattcag tggttgatag atatggaaga atttgaaaaa tccatgattt
   115441 agcctaaagt tgacagctga atgggtttat cgttaaattt aattttatac tgttgaatga
   115501 taaagagata tataacttta attaatataa atgagaagaa aaatattgat tatctgaacc
   115561 caaacatcat ataaaaatgt taatattaca ggttatgagc atattataat gtaattatga
   115621 tttatagcac tccgttgtct ttctgattag tttacccatc accaccatgg ttggtattgt
   115681 ttaaaagtgc taatattttg tgaaaacaat ttcagacggg atgctcatat aatcgaacta
   115741 acggagggat tacggttcga gggttaccac aaaaaaataa aatttctgaa tggatggata
   115801 gcgcactaag tggctcaacc agcgattaac ccaacacctt tattacccgt tctttaaatg
   115861 taaatattgg agataagctt ttcaaacact gtagtaaata tttaatcata aatcacaaca
   115921 accagaaaat aacccgaata aaaatatttt aatttttact taagcgggca agttttaatg
   115981 ccacagccac ttgcatggtg ttttttactt acctttgcag caaaataaat attgtatgtc
   116041 gtacgttacg aaagaaaagt tgtttagtaa ggcttggttt aaagcctaca ccctaataac
   116101 aattggtgca tttgtgttag ctgctgggtt cgtgctattt atcgatcccc atagaattgc
   116161 acctggaggt gtttacggaa tcggaataat tgtacactac cttacccagg gcgtgttttc
   116221 ctgggcgccc gaaggatttc cgattgggac ggtaggcctg ctactcaaca ttccgcttac
   116281 cattttagga attaaaattc ttgggccacg ttttggtgtt aaaacggtgg tgggctttat
   116341 tttgtcgtcg gtttttatag atacccttca catgatttac ggctaccatc ctcttgtgga
   116401 cgatatgcta ctttcgtcaa tttttggagg ggttctaatt ggcttcgggc ttgggctgat
   116461 ttttaaatca aaggcaacca gtggcggttc cgacattatt gctatgataa ttgcaaagta
   116521 caccaggtta ccattaggac agctgatgat ttatgttgac tctaccatcg ttctaatagc
   116581 acttgtagca tttaaggatt ggaaaatccc tctctactca tgggtggtga tctacataac
   116641 ggggaaggtg atagatatga ccatagaggg agccagctac gataaagccc tgtttatcat
   116701 tagcgaccgt tacgaggagg tgaggtcaaa aatcatcaac gatctcgaaa gaggcggaac
   116761 gcttattagc gttaagggaa tgtatagcgg tgaggaaaaa aagttgattt tcacgaacgt
   116821 ttcccgcaga gaggttttta tgcttaagga ttacattcgt gaaatagatc ctaaggcttt
   116881 tattacagtt attgatgcca acgagattct tggcgagggg tttaaaccaa tcagcgaggc
   116941 tctttagttc tgcaggcaaa tgttaaacaa ttcaaccagt tggtttataa tccatcacat
   117001 aatttaaagg tcagcaacag tgaatcaatt actttaaatt aagaatggct acagaataag
   117061 ccctgttatt gtgctgtgaa ataaacttta tatctttgaa gcctcaaatt ctttgaaacg
   117121 gtattgcccg ggtggcggaa ttggtagacg cgctggtctc aaacaccagt ggtagcaata
   117181 ccatgccggt tcgatcccgg ccccgggtac ttatccaacc accagtttta cttaaaagtt
   117241 gctttatttt ctccgtttac tgtacagcta taattcaaat ctatagtagc aaaaacgctg
   117301 caaacgttag ccctacatga ataagacgac cagttttaat gaccccaaac agcttcagtt
   117361 tgacagacgt tatctggaaa tggctaaaat ttgggcaaaa aactcttact gtaagcgtag
   117421 gcaggtggga gcgcttattg tcaaaaatag aatgataata tctgatgggt acaatggaac
   117481 accctcaggt tttgaaaaca tctgtgagga tgagaatggt attactaagc cttatgttct
   117541 tcatgccgag gcaaatgcaa ttaccaaggt ggctaaatca ggaaatagct cagaaggagc
   117601 aaccctttac gtaacctcgt cgccgtgcat ggaatgcgcc aagctaatca ttcaggcggg
   117661 cataaaacgg gtagttttct gcgacgaata ccacaatccc gatggccttt cgcttttgag
   117721 tagagcagga attgatgtgg taaaactcga catagaggaa ctttaggctt tttaaagtta
   117781 tatttctaaa agagtacaac ctgatggttt taataccaat aacttcagaa taaattactt
   117841 gaatagaatg gcataccaaa atagcaaaaa ggatatttac tatcctatag tcatagccct
   117901 agtgctggcg gcaggaatac ttattggtca acgatttaat gggaaaagcc aaaagcccgg
   117961 aacgctggtt tataaccgaa ctgataagct gagcagcgtg ataagctaca tagaagagga
   118021 gtatgtagat tcaatttctg tatctaccct ggtcgaaaac actatacctg ctatacttaa
   118081 aaacctagac ccacactcca tttacatacc agcttcggag ctgcaatctg taagcgaacc
   118141 tctcgaaggg ggctttgacg gaattggaat tcaattcaac atgtcgaatg acacagcagt
   118201 agttgttaat accataaagg gaggcccctc ggaaaggctc ggaattttgg ccggtgacag
   118261 gattgtaaag gtcaacggaa agctgattgc gggagtaaaa tttccgatgg acagcatccc
   118321 cattctcttg aaaggcccta ccgggacaaa ggttaaggtt gaaatcaaaa gaaaagacgt
   118381 tgacgagctt attgatttcg agataacaag ggataaaatt ccaataagta gcgtggacat
   118441 tgcctatatg gtaacacccg agatagggta tattaaaatt acaacctttg ccaaaacaac
   118501 ctataaggaa tttctagatg caatggctaa gctcgaaagc cagggaatga aaaaacttat
   118561 acttgatttg cgaagcaatg gtggagggta catggatgct gccaccaaaa ttgccaacga
   118621 gttcctaaag ccaaatcagc tgattgttta cacacagggt aaaagccgaa cccgtcagga
   118681 tgtttactct actggaacag gaaagtacca ggatattcct ttacaggttt taattgacga
   118741 attttccgct tcggcaagtg aaatttttgc cggagcgata caggataacg acaggggaca
   118801 gatagttgga aggcgttcat ttgggaaagg cctcgttcag gagcagatat ttttttccga
   118861 tggatctgcc ctaaggctca ccattgcccg ctactacacc ccatcgggta gatgcattca
   118921 aaaaccctac acacctggcg acgataacta cttcaacgat atcacaagcc gatacctgca
   118981 tggagaattt gaggttgcag atagcataca gttcgccgac tcgctaaaat actacacagt
   119041 aggcggaagg actgtttatg gaggaggagg cattatgccc gatatttttg tcccactcga
   119101 taccaacgga attaccccct acctgcagca agtatcccga agaaatctca tttaccggtt
   119161 tgccttcaac ttcaccgatc agcatcggaa agaaactcga ggtttaaagg actttacctc
   119221 ggttgatagg ttcctgaata aatttgacct gctctccgac tttataaagt ttgccgagca
   119281 aaacggtgtg cccagaaacg aaaaggaaat atcaatttca aaaaagattc tggatgcaca
   119341 agtaaaagca actatcgccc gaaatattat tgataatgaa ggattttatc catacattag
   119401 gaatatagac gaaacattgc taaaagcaat tgggctaatg gagcaaagcg ttagtcaacc
   119461 tacaacctca gctcttcata ggagtgttaa ttcacgtgtg cttaatcagg ctcagctata
   119521 aacctgggta agaaacgatg taatggcagc ggttgtttga tccttctcct caacaaaacc
   119581 catatggccc gagttgttta gaataacggt tcgggctttc ttgtgccttt ccattaaggc
   119641 ctctgcaacc tccatagaaa tcaggttgtc gtgttttcca aagatgaaga aaacaggaat
   119701 gtttgcattc tcaataacat aattcctatc ggggcgtttt gccattccgt tcagcgcccc
   119761 gattattccc tggtcaggtg tacttagtcc tattttgatg aatttatcaa caatgtcgtc
   119821 cattttctct aaattctggt tggcgaaaag gcgaggaata cttaggtaaa caaggtcgcg
   119881 ctttccacca tttttaacgg tttcaatgtc attcatcctg ctctgacgct tttccgacga
   119941 gtctgcgtta gccgatgagt gaagcaagca aaagccgttt aaccagttag ggtatcgttc
   120001 tgcaaaggca agcgtggtat aaccacccat tgagtgtcca accataaagg cttttttaat
   120061 gtcgagggtg gtcataacag cgctaatact atcggccata agatccatac taatggtttg
   120121 atgattacaa gccgactttc cgtgaccggg caaatcgacc cgtagaacgg tatagttttt
   120181 ctccaaatcg ggaacaatat cttcccaaat ctcaaggttt tccagatagc cgtggacaag
   120241 aataaccgcc gggccttgtc cgcttaacct gtagcgaatg gtgcaaattg gtaactcaac
   120301 gtagtcttcc ataattcaga aaatgatttt atacaaagtc taaacttatt tatcctaacg
   120361 ctgccagggt tctaaaccct gacagcgttc aatatagcaa attgattcca gataaaacta
   120421 atacaaacca attaaaaaac aaattgcaac aagatctaat gggtttaatt tttgaactca
   120481 atgtcaaaaa aacgccacta atctaaatat tagcggcgtt ttaaaatata acgaactatt
   120541 tgttaaatat aaacagaaat accaatttgc cattgcgatt tcaaatcctc tttaaattca
   120601 tatggccaat tctttggaat cgatttctct attgcattta ttggactttg attgtgtaaa
   120661 gaaccccaaa ttgacattgc cctaaaatag ccctcctcct tcttccaggc aagtctaaaa
   120721 cctgccttaa gcgcaaaata accattaaag tggacccaat ttgaagtgct agttttggtg
   120781 ggttcataaa atttcatatc aacattatgg ctagccaatc ctaattccaa ttcaataaat
   120841 aacctatcga aattgctatc gtattgctca aaaattatca taggcgatac accaacaaac
   120901 aaactatttc cagtgtagct atatagcgat tcgcctccat cataaacaac accagtttca
   120961 tctgtaagac ttctatggcc tcttgttgaa gcatatcctc cacgaattgc aagtcccaaa
   121021 attccagact cagtaaactg tatttcatat ccacccgata ttcttgcttg tataggcata
   121081 gttccacctt tctcaagcaa cggggttcct gcactaacat ctaccaacca agatttttgg
   121141 tacagccctt ggcctgtt
//
LOCUS       DAOSHM010000002        91650 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_188_length_91650_cov_19.989104, whole genome shotgun sequence.
ACCESSION   DAOSHM010000002 DAOSHM010000000
VERSION     DAOSHM010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 91650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 91650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..91650
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_188_length_91650_cov_19.989104"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            421..1305
                     /locus_tag="PK839_00505"
     CDS             421..1305
                     /locus_tag="PK839_00505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG71244.1"
                     /translation="MLYLPLCILSSAVILVVFKIAERMKINTLSIILINYIIACLAGF
                     FLFRGEGAFSGQFSPGSIAFMVCLGVLLVVVFRLVGLSTQRAGIAATSVASKMSVVLP
                     ILFSIWIDPHDKLTSTKLAGIVVALVAVILSVYRNDNPRDSLRKIYLPVLIFFGIGTI
                     DSSVKYAQATFVTGELNPLFNFVIFGVAFIAGFAMVAFSKPALKDLQHLKTWLVGVVL
                     GLANFGSMYFMIAALNHINPATGKPAQGSVVFGINNIGVVVLGVLLGYTFFKERPTRL
                     NWSGIALSVVAIFILMNS"
     gene            1305..1916
                     /locus_tag="PK839_00510"
     CDS             1305..1916
                     /locus_tag="PK839_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HOG71245.1"
                     /translation="MQADDTYQTIELKAEGLYKEKGSRFIALAFPVSSEVQVKAILDD
                     LRQEYHDARHHSFAYIVGYKGERWRANDDGEPSGTAGKPIYGQLLSFNLTNVLVVVVR
                     YFGGTKLGVSGLINAYKTAACNALNNARIVTKTIDQTFSVFVDYPLVGEVMRVIKDLS
                     IQVIEQQFDTRCMVRVSVRQSLCGEFTSRINKVESAWIDSVDQ"
     gene            1980..2888
                     /gene="pyrB"
                     /locus_tag="PK839_00515"
     CDS             1980..2888
                     /gene="pyrB"
                     /locus_tag="PK839_00515"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HOG71246.1"
                     /translation="MKSKSLVSINDLSRDEMLRVLDLAEEFERNPSQPILQGKVVATL
                     FFEPSTRTRLSFESAVNRLSGRVIGFSEASSSSVSKGESLKDTILTVANYSDLIVMRH
                     PVEGSARYASEVSPVPIINAGDGANQHPTQTLLDLYSIRKTQGTLNDLKIVMVGDLKY
                     GRTVHSLLMAISQFNASFVFIAPPELQMPQEYKLFLKESGLKFEEHTDLADNIVDADI
                     VYMTRVQRERFSDPMEYEKVKNVYVLRNHMLANTKPNMKVMHPLPRVNEISIDVDDSS
                     KAYYFTQALNGVYTRMAIMSLILGLK"
     gene            2974..3432
                     /gene="pyrI"
                     /locus_tag="PK839_00520"
     CDS             2974..3432
                     /gene="pyrI"
                     /locus_tag="PK839_00520"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009127487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HOG71247.1"
                     /translation="MKDKKHLSVSAIENGTVIDHIPAQNLFKVISILGLDRIPNQITF
                     GTNLESQRLGRKAIVKIADKYFEREEINRIAIVAPHAKLNIIRDYEVVEKMLVEVPDH
                     ITGIVRCVNPKCITNFENVAPKFEVVDKKNVSLKCHYCEKITDQEHMEYR"
     gene            complement(3575..4909)
                     /locus_tag="PK839_00525"
     CDS             complement(3575..4909)
                     /locus_tag="PK839_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF389 domain-containing protein"
                     /protein_id="HOG71248.1"
                     /translation="MKPQSVISKFFHYFNLESELDSYDRIHESIVKDVVFRGTNLWIL
                     MFAIIIASVGLNMNSTAVIIGAMLISPLMGPINGMGYSIATYNMKLFKISLKNYGFAV
                     LAGLFASTLYFSISPISTAHSELLSRTTPTIYDVIIALFGGLAGIVAISSKQKGNVIP
                     GVAIATALMPPLCTAGYGLATGQFNFFFGALYLFTINTVFIGISSVAISQILKFPIIA
                     DMDVRQKKSINRWISTVIIVVLLPSIYFGYRLVQNEKFNLNASRYISNVSSFEGNYLL
                     RNNIDVRKKIITLTYGGVSLTEKQKEAITQKASNFDLDDAKILIEQGLALNSSNETDS
                     EVIKLRSELTRLNMIIRDKDAQIDSISRKRQLGKTLLLEIQAFFPKISSCSYAETFQY
                     KTDSSQPDKTTVVFFGITSGITPAEKKQISNWLNARLNQDDIKVFWEYVPKN"
     gene            5437..6558
                     /locus_tag="PK839_00530"
     CDS             5437..6558
                     /locus_tag="PK839_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005633330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOG71249.1"
                     /translation="MTAFSKRLIRISTTVAIALLLVGIILYPKVKDASNSDSKDKVPG
                     RMAMRGGGGQPLFANGFIIVPTSMSELISTTGSLLPDEEVDLSFETSGKVVAIYFEEG
                     TKVKKGDLLAKINDAPLQAQLQKLQAQRKLAEEKLFRQKQLLERDAISRESYDQVSTE
                     LQAIEADIMLIKARIAETELRAPFDGIVGLRLISEGAYTSTQTPIAHLVKVSPLKIEF
                     SIPERYAGEVTPGYDISFVIDGIESKFTGKVYAVAPKVDMATRAIVVRAMYPNPNEEL
                     KPGRYASITLQLSKIENAVSIPTEALIPEMEGEKVFIYRNGKAEQVRVTTGLRTESRI
                     QVRTGLNFGDTLLTSAILQLRQGIPVQLDTIITNAINLQ"
     gene            6564..9632
                     /locus_tag="PK839_00535"
     CDS             6564..9632
                     /locus_tag="PK839_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOG71250.1"
                     /translation="MSLSSLSINRPVLSTVFSLIILLLGAIGITFLGVREFPSVDPPI
                     ISVSTSYPGANSDVIETQITEPLEQSINGIPGIRTLTSRSSQGSSRITVEFELSVDME
                     TAANDVRDKVSQAQRLLPRDCDPPTVSKADADARPIMNIAINSKKRSLLELSEIADLT
                     LKEQLQTISGVSSVGIWGEKRYAMRIWLDPAKLAGYQMTPLDVRNALNRENVELPAGS
                     IEGANTELTIRVLGLMSTPEEFNNLILKRSGEQLIRVRDIGRAELAPEDLRSIMKMNG
                     VPMVSVVVIPQPGANQIEIVDEVYKRIDFIKKDLPDDVNIVVGFDNTQYIRSSIKEVQ
                     TTIYIAFFLVVVIIFAFLRDWRTTLIPILVIPVSLVGVFFIMYLAGFSINILTLLAIV
                     LSIGLVVDDAIIMMENIYVKIEQGMTPLEAGVKGSNEIFFAIIATTITLISVFFPIVF
                     LEGMTGRLFREFSIVIAGAVAISAFVALTFTPMLSTKLLKTRHTRSKAYSYTEKFFTS
                     LNSGYRNSLSSFLKHRYLPVVILVVAAGLIVLLWKSIPAEMAPMEDRSQVSLSVTFPE
                     GTTYDYSAAYVEDVAALVDKLVPEAEKATSRAWRGGASMDVVLIPPSERDRSQQDIAN
                     MLTAELRKKTKARVNVIQQSTFGGQRASLPIQYVLQAQNIDKLREILPAFMAEVQDNP
                     TFTMADVNLRFTKPELQVEINRDKANMLGVSTQNIGQTLQLALSGQRFGYFIMNGKQY
                     QILGELPRATRNEPLDLKSLYVRSDAGQMVQLDNFVEVKESTAPPQLYRYNRFVSATI
                     SAGLAEGKTISQGIEEMDKIAAKTLDDTFRTALAGDSKDFTESSSSLMFAFAMAIVLI
                     FLVLAAQFESFKDPVIVMMTVPLALAGALIFMWYFNITMNIFSQIGIIMLIGLVSKNG
                     ILIVEFSNQRKNAGMSKLDAVKYAAAARFRPILMTSLSTILGILPLALGLGEGAQSRV
                     AMGTAVVGGLTVATFLTLYVIPGVYLLISSESAAGKAANSKGREVEGIEQ"
     gene            9744..11120
                     /locus_tag="PK839_00540"
     CDS             9744..11120
                     /locus_tag="PK839_00540"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOG71251.1"
                     /translation="MNGKFDLMNQTTPSALATPMAKRIFTITLLICLCSGLSKSQELF
                     DLSSCIKAGLEQNFSILIARNQEKIASNNYTVGNAGYLPTLSGSGRYGGTINTTHQSF
                     TDGSESEANGVNNTSGVVGVDLNMTIFNGFKVKATYQKLNEMKQLGILNTQLEMENLA
                     AEIASEYFFFIQQLNLYNNMAYAVELSHERVRIDEERYLLGSSSKVDLLQSIVYLNSD
                     SSRFAKQKEVLRASQVRLFKLMGNVDLGYDIRPKDTVISINDGLLYDDLLASTMDQNT
                     SLQIARRNTTISQYDYKIIASRSYPYLNLTSGYDYSFNDYETGTVKSQQIHGLSYGLT
                     LGVDIFDGFNRKRERTNAAIEIENREHEYSDVVQGVKADLLTIYYAYQNNLKLLKLEE
                     QNLSVARENLEIAMERYRLGDLSGLELREVQKSLLDAEERLISVKFQAKLAEISLFQI
                     SGKIMSYL"
     gene            complement(11363..12133)
                     /locus_tag="PK839_00545"
     CDS             complement(11363..12133)
                     /locus_tag="PK839_00545"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HOG71252.1"
                     /translation="MSFIQSIIIAIVEGITEFLPVSSTGHMILASTLMKIQENDFVKT
                     FEIFIQLGAIMAIVMLYAKKFLQSFSIYVKLGIAFIPTAVVGYIAYDFIKGYLFNSIV
                     VAVSLIVGGIVLVLIDQKLTTKKSKVEVVEDISYKNSFFIGLIQCFSMIPGVSRAAAT
                     IIGGVFNGLDKKQATEFSFLLAVPTMIAASGYDLLKTPISFTSHEVVLLATGFVVAFF
                     TAWLAVKVFLKIVENYGFKYFGYYRILVGAIFLLFFRF"
     gene            complement(12400..13035)
                     /gene="msrA"
                     /locus_tag="PK839_00550"
     CDS             complement(12400..13035)
                     /gene="msrA"
                     /locus_tag="PK839_00550"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:NF013766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HOG71253.1"
                     /translation="MKRVAIVASWLLITQFSMACESQTNQNHKEMKASASETITLGAG
                     CFWCVEAIFSRIDGVLKVESGYSGGSAVNPTYREVCNGNTGHTEVVQVTFDPEVIPLA
                     KILEIYFKTHDPTTLNRQGADVGFQYRSVIFYHSQEQKKIAEEIKAELEKARIWNSPV
                     VTAIEPFKNFYKAEDYHQEYYENNKNQPYCRMVITPKVDKLEKVFADYLKH"
     gene            complement(13167..14357)
                     /gene="mnmA"
                     /locus_tag="PK839_00555"
     CDS             complement(13167..14357)
                     /gene="mnmA"
                     /locus_tag="PK839_00555"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HOG71254.1"
                     /translation="MKKTEMERVVVGLSGGVDSSVAAYLLKEQGYEVVGMFMRNWHDT
                     TGVLKGNCPFDDDRDFAEMVARKLRIPFEFVDLSNEYRQRVVDYMFNEYSKGRTPNPD
                     VLCNREIKFDAFVKAAFELGADYVATGHYCRKQEIEVNGQTYYRLLTGTDSNKDQSYF
                     LCQLSQQQLSRALFPIGHLLKPEVRKIASELGLATAERKDSQGICFVGKVDLPVFLQQ
                     KIKAVEGDIVEIPRAFYSSQPVISPDDLEALCKPTFYSKADGNLVGQHHGAQFFTIGQ
                     RKGLNVGGKSEPLFVIATDVLQNVVYTGMGDDHPGLYRKGLFVSQNDVHYIRPDQKLR
                     PGQTARLMVRIRYRQPLQKATLLSRDNGIYIIFDNPQRGVTAGQFAAWYDNEELIGSG
                     VIAM"
     gene            complement(14482..16728)
                     /locus_tag="PK839_00560"
     CDS             complement(14482..16728)
                     /locus_tag="PK839_00560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG71255.1"
                     /translation="MYNLRHLSYLYFLILIFFPTKSFSQDWNLLFTLETDAEFYMADG
                     NYSRAADTYLKALKKYPESCNLMFKVGYCMLKTDDRKAEALSYLEEAAKKVSDKYDAR
                     SLKEANSPPEVLYHLGISYMHQNNFDKAKAQFEAYKKYVNPNDAYVLQQVEQNIKSCE
                     TAKAMMDSPIGLNLNRLEEGVNNDQSNFDAVLSGDGKTMAFTSRSTSGNKVFISFMDK
                     GKWSIPKDITRNLGSSYLTTSFLSYNGEELYLIDNDLKNSEIVVSFLQKNKWSNPVKA
                     AKPINSKANETHVCVTRDGNTAYFTSDRKGGQGGFDIYKVTISGDKWGEPVNLGPNIN
                     TPLDEATPFLTPDEKYLFFSSQGHNSMGGYDIFYVNLEGSSNVKNIGYPLNTPDDNLF
                     YFPTSLTSGYISTFSPNGLGGLDICSVEIIPQVDVKINIMLADKAPADKSYTVSIIDT
                     KDGKPVSSVNQNGKKQILQKLSPGTFTVAASGVEFDSTSSLLEIPSKPDKNEYQISLL
                     LNPSQPKPEPVAEVSQPANDIPIQKEVKPVPVVVEEPKPEKPVPASVEKPKQEKSTAA
                     SVPVNPSPKPEVKKREMEKPRPVHVAVTNLSLAAGTSTSLAHPTYSVQLMACKQPVDY
                     DYFKLDSIFVSISPEGYYRYSVGYTLSVQQAEELLARVKTLGYTNAYVRINKEQPSYT
                     IQLMALTKPKRLNQLNVIGSTMVYRGCDGIYRYCTGSYSTPEEAQNDLASFKNAGYSD
                     AFIRHLGR"
     gene            complement(17044..18225)
                     /locus_tag="PK839_00565"
     CDS             complement(17044..18225)
                     /locus_tag="PK839_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOG71256.1"
                     /translation="MSEKFFIEVNCQQKNHDGERICGDVFYSKRLQDEERTIIVLSDG
                     MGHGVKANVLATLTSTMAFNFTREHKEVNTIAEIIMNTLPVCSERKMSYSTFSIVDIE
                     HNGDTRILEYDNPECFIMRGTEPLIPEWQCIILNSEQNAGKELRYCSFTPQKEDRIIL
                     WTDGVSQSGMGTPDYPFGWSDGAREFVEKMVKNEPDISARKLSAKVVNMAYVNDGYHP
                     KDDTTCATIYFRHPRKLLICTGPPFEKENDSMLASIFNSYEGKKVICGATTVDIIARE
                     LKREVVDSMEFTDPDLPPTSQMDGADLVTEGILTLSKVNTILDSYNETVTLGNGPAEQ
                     LVKLILESDIIDFVIGTCINIAHQDPNLPVELEIRRTVVKRIANLLQEKFLKEVKLKF
                     L"
     gene            complement(18252..20132)
                     /locus_tag="PK839_00570"
     CDS             complement(18252..20132)
                     /locus_tag="PK839_00570"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HOG71257.1"
                     /translation="MSQLFEISEETCINCYACVRVCPVKAIEVKAGQDFARILQNRCV
                     GCGSCLNICPVNAVSYRSSKKNVLELLKGNEPVAAICSPSISGEFVDITDYRKFVQMI
                     KTLGFTYVCEVTFGVDLVAREYKKLFDDFKGKYFITSMCPVVVSMVEKYHPEMVNNLA
                     PIISPIIATAQAVHNQYGSNVRIVHIGPCIQSKEEAKLYNGKSQIDEVLTFVELRQLF
                     EEFNINESNLEYSEFDEPIGYKGSLYPIGRGILQAVDISEDLLTGTVISANGPNNTLQ
                     ALEQFETSAEMINRHFNLFYCEGCLMGPGTSKGGKKFIRRTMVVNYANKRLLTFNRKK
                     WEENISRNEKNSYTRDFTPDDQRLPLPSQQKLEEVLKVIDKEFVGKETGCEACGYASC
                     YDFASAVASGLAHTDMCLNFTLKNRQEYIKSLRSTNEKLAKTQEALRESEKKARIEQE
                     AAREASETVTTMLKKLPAAVVLIDKKLKIIQSNQSFIDLLGDDALEINEIVPGLVGAD
                     LKSLVPYNIHNLFTYVLQNNSDILNRDVHYRDKLLNISVFTIRPNSIVGAVIRDMYAP
                     AVQKEEVVKRVTEAIDRNLAMVQKIGFLLGEGASEIEQMLNSIIQTYNEGVAKAENSK
                     TK"
     gene            complement(20151..20402)
                     /locus_tag="PK839_00575"
     CDS             complement(20151..20402)
                     /locus_tag="PK839_00575"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HOG71258.1"
                     /translation="MEKKNEIVICLGSSCFARGNKATLKTIQKYIDDHHLREKVYFHG
                     GHCFGRCAEGPTVTVNGKIFTGVDEYNILDILNSELSEY"
     gene            20682..21143
                     /locus_tag="PK839_00580"
     CDS             20682..21143
                     /locus_tag="PK839_00580"
                     /inference="COORDINATES: protein motif:HMM:TIGR01958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOG71259.1"
                     /translation="MKRSIEAILEQYDHAGMDSLIPILQEIQNKTGCLNEESIVMVGK
                     HLGIPTSKVYGLATFYNQFKFEPKGKFHILVCNGTSCRLNGSKWIIDFIEKNLKIKNG
                     ETSRDGTFSLEVISCMGACESGPLISINEEYYCNLTPAKLGEVLAGLAESE"
     gene            21243..23189
                     /locus_tag="PK839_00585"
     CDS             21243..23189
                     /locus_tag="PK839_00585"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HOG71260.1"
                     /translation="MNHSYTLADKTFFCEQVLPLLHAELNSDTQKHLSTYRHERVKKT
                     VIYIGITTSSVVAGALKTLKAIEGYLEENSVDAIIVPVGSYGLCSFEPIVEVQLPGKK
                     RVAFRNVTYDRVEFLLDGAFNSFVQPDTALFQHNSDILEPWPDIPVKDDFPFFAKQDR
                     LLLRNIGIINPENLLEYISFGGYRALAYTLRNQTSDSVCRIVELSGLRGRGGGGYPTG
                     LKWRKTLSVAATERIMICNADESDPGAFMDRLLVESDPFRIIEGLTISAYAIGANKAI
                     IYIRNRNKLAVDRLTLAMEQAYDFGLLGYNILDSGYSLDISIFQGPGAYVCGEETALI
                     QSLEGKRGTPSIKPPYPNEVGLNGKPTVVNNVETLASIPDILLNGADWFMKIGTATSK
                     GTKIFSVNGKASLTCVVEISMGRPLSAIVELAGGVKPDCTLKAIHLGGPSGVSVTPDE
                     LYLPVDFDELKVHGIPLGSGGVQILDQSVCMVDLTKFFMHFIRNESCGKCIPCREGSS
                     RMYQILNEISRRPDSSDKHHTLERFKGVIQLETLAEVMHDTSLCGLGQTASKPLLKAL
                     KAFRDEFEEHIFDRSCRAGVCRDLRTFYIDTEKCTGCAACAKKCPTNAIIGTPRSPYF
                     IVEDKCTGCGSCMEVCKFASVFYR"
     gene            23340..25064
                     /locus_tag="PK839_00590"
     CDS             23340..25064
                     /locus_tag="PK839_00590"
                     /inference="COORDINATES: protein motif:HMM:TIGR02512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016695 - oxidoreductase activity, acting
                     on hydrogen as donor [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase, group A"
                     /protein_id="HOG71261.1"
                     /translation="MNFNIEVNNRQILVHRGETILNALRGNGIKIPTLCNMKELSPTG
                     ACRLCVVEVEGRERLVTSCSQLVEEGMRIKTHSSRVVKARKTLVELLLANHPDDCLYC
                     ERNGRCELQSLAVELHVRERHAQNKTFSQKLDLSSHSIIRDPSKCVLCGRCVRICNEL
                     QSVSTFEFVKRGCNMYIGTAMSRDLTFSNCIACGQCVMSCPTGALSERSNVDEVIDAL
                     NNPNLIPVVQVAPSISVSLASELGLKPGKDLNGVLNAILRKIGFRYVFDSAAAADIAV
                     METATELLKRRENGEKHPLISSCCPAWVKFMEQYYPEHLSWLSTTKSPQQVMGALIKS
                     YFAPSYGINPAGIFSVSVMPCLAKKIEARREEMTTKGVSDVDTVISTRELVQLVELYG
                     IDVQNVESQQPDSPFDIRSSAGKLFGVSGGSSEALARTLHYMNTDTELDNFKIAELRS
                     SSGIREFKLTTGKQEFVFVVLSGMKSIHSFMAELINGNVRADYVEVMACPGGCVNGGG
                     QPFAMDPKEIRQRTKSIYELDEADVVKSSHKNPVVKSVYDDFLKEPCGELAQKFLHTS
                     YYKRDVLL"
     gene            25143..27131
                     /locus_tag="PK839_00595"
     CDS             25143..27131
                     /locus_tag="PK839_00595"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HOG71262.1"
                     /translation="MQEENRKLVYTIKELCRVCYTCVRECPAKAIRIINGQAEVIAER
                     CIGCGNCVKVCSQDAKVYLQTRMEVRDLLQSEDKVAALVAPSFPAEFSEIDDYRKFVG
                     MLRQLGFSYVFEVAFGADLVARRYKELLEDRSGKGYISSDCPAIVTYIRYYHPDLTEY
                     LAPVVSPMIAISRVVRKKLGNGVKLVFIGPCIAKKAETDEVDEALTFRELRGMLEYAG
                     VTPEAATPSEFDLPRAGRGAIFPVSRGLIQTVRVNEDLVEGNVTVAEGRMGFQEAIKE
                     WEIGNLKGQNLELLCCDGCIMGPGMSPGGKRFERKTQISRYARQKLSELEHDEWLYSM
                     NEFRDIDVSRSFQRDDRRMPIPSDDQINRVLASMGKFSPRDQLNCGACGYETCVEHAI
                     AIIDGLAEVEMCLPYAIEELHISVDNLAISNEKLAKMQQALKQSEKLAHMGQLSAGIA
                     HELNNPLGVVLMYSNILMDECKPDDPLRKDLELIATQAERCKKIVGGLLNFARKNQVK
                     VERVDIRKLAAESVTSVVIPKTISTQVVCKANNTEAYLDYEQMMQVLTNLNKNAVDAM
                     PNGGVLTVEVDENESDVIFRVSDTGTGIPEENMDKLFTPFFTTKGTGKGTGLGLATTY
                     GIVKMHSGKIEVDSNTDPAKGPTGTTFTIVLPRKPTLG"
     gene            27158..27556
                     /locus_tag="PK839_00600"
     CDS             27158..27556
                     /locus_tag="PK839_00600"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOG71263.1"
                     /translation="MPTPAKKTILIVDDDVDYLYQLKFQVEKMGFETVTAESQHEAET
                     LLNTIKPDLAILDLMMESEDSGFILSYKLKRKYPDVPIIIATAVSAETGMTFGINTEE
                     ERKWIKADLYLEKGIRPDQLHREIVKLLKL"
     gene            27563..28699
                     /locus_tag="PK839_00605"
     CDS             27563..28699
                     /locus_tag="PK839_00605"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HOG71264.1"
                     /translation="MEVVDVLVVDDEPGIRSGVSRILKNYTINYPFLEDDISFNVVEA
                     ATGEEAIEIIQRKPPAIVLLDNKLPGIQGIDVLQFISAHEPNILVMMITSYASLELAV
                     KATNIGAHDFVPKPFTPQELRSSIENITKHYFLRQMTRKLHREGKQVRFEFLTMLSHE
                     LKAPLNAIEGYLRMMQEKQLGESLDDYQEIIDRSLSRIQSMRNLIMDLLDLTRVESGK
                     RERDLQDIDLTLVARLVVETMMPMAIQRDVTLTLESDEPVVVKADADDMEILLNNLIS
                     NAIKYNVEGGWVKCKFSKTPTYATCSVSDSGVGISADDIPKLFHEFTRIKNPLSRNVS
                     GSGLGLSIVKKIADQYGAEIKVESTLGKGSTFTLQFPVSAENPA"
     gene            28973..29596
                     /locus_tag="PK839_00610"
     CDS             28973..29596
                     /locus_tag="PK839_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HOG71265.1"
                     /translation="MKLPEKTVERLSEYRRTLLSCIAKGKTHIYSHELAGIHNITAVQ
                     VRRDLMLIGYSSIKKKGYDTQELADVIGALIDHEKGLNVAVVGMGNMGRAITSYFNGK
                     RPKLNIVAAFDVDPNKVNRVIAGVNCFHMKDLLEISKRYEISIAIITSPPDSATQVAE
                     QLVSAGIKGILNFTTIPLNVPDTVYLEEFDMITSLEKVAYFVKTKDE"
     gene            29632..30576
                     /locus_tag="PK839_00615"
     CDS             29632..30576
                     /locus_tag="PK839_00615"
                     /inference="COORDINATES: protein motif:HMM:NF018299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin kinase"
                     /protein_id="HOG71266.1"
                     /translation="MQVHYGFDNVGYIKNPVVTTGSFDGVHIGHKAILKRINDIARGI
                     DGESVLITFYPHPRKVLYPENAGKNLVFILSQKEKIELLSKTGLDHLIIVRFTLEFSH
                     ISSVDFIQHFLIAKLHARFVVVGFNHHFGHNREGDFEELKRLGLQYNFGVEEIPEQDI
                     QQETVSSTAIRKALLEGKIQRANAYLDHHFIIMGTLGKGAHIFQRAGFPTLTVQIEEA
                     GKLIPHEGIYAVTLEWNSTHYRAMAIIWLDNYGSNEPVLGPLNVDVHIMEFDNILSED
                     DAKLFFQKQLADHVDIADEERLTSQLSAACSSLDELIF"
     gene            30749..30994
                     /locus_tag="PK839_00620"
     CDS             30749..30994
                     /locus_tag="PK839_00620"
                     /inference="COORDINATES: protein motif:HMM:TIGR03959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-only hydrogenase system regulator"
                     /protein_id="HOG71267.1"
                     /translation="MERRIGAALILVENRGSVEKLNQLFSAHSSVIVARQGIPLQQRG
                     VSVISLVLDGTSDEISALTGPVGKLDGVQVKTLMMKG"
     gene            31226..32653
                     /gene="hydG"
                     /locus_tag="PK839_00625"
     CDS             31226..32653
                     /gene="hydG"
                     /locus_tag="PK839_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HOG71268.1"
                     /translation="MKFNPEVYSIPDERMKPFIDPDEIWGYINSAKPTRERVREIIAK
                     SLDKHRLNLEEVAVLVNTSEPELVEEILQGARTLKQKVYGNRIVLFAPLYIGNKCTNN
                     CKYCGFRTSNTEAVRVTLSDEEITKEVEALEDNGQKRLILVYGEHPEYSADYIAHTVR
                     LVYGVKKDRGEIRRVNINAAPLSVEDFLKVKKAGIGTYQIFQETYNPEAYSWYHQAGK
                     KRDFEWRLTSLDRAQEAGLDDVGIGALMGLYDWRFEVLGLVRHTNHLEACYNVGPHTI
                     SFPRIKSASGLDITNQYFVSDDDFVKLVAILRLAVPYTGMILTAREPVHVRQKVLQFG
                     VSQIDGGTKLELGAYIESKNEKQNLNREQFEIGDSRSLGEVVDDLVEHDYIPSFCTAC
                     YRLGRTGEHFMEFSVPGFIKRYCTPNAVLTLTEYLVDYATPEVKDKGYRLIDKTLTEL
                     DGNQNVEEIKRRMERIKAGERDLYF"
     gene            complement(32954..34378)
                     /gene="pyk"
                     /locus_tag="PK839_00630"
     CDS             complement(32954..34378)
                     /gene="pyk"
                     /locus_tag="PK839_00630"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HOG71269.1"
                     /translation="MLKRTKIVATISDQRCEVSFIKSLYDAGMNVARLNTAHMNPSGA
                     KILIDNIRKVSDHIAILVDTKGPEIRTSSKGAEVKVQQGMTVKISGDPNGESGGDNIF
                     VSYPNIHEEVPVGNAVLIDDGEIELRVRSVSHDALVCEVENGGVIKLRKSVNLPGVHI
                     NLPSLTQKDKDFVQFAVENRVDFIAHSFVRNKHDVLEIKEILQQQKSPIKVIAKIENQ
                     QGVDNIDEILDHAYGIMVARGDLGIEIPSEKLPIIQNRIVRKCIESKKPVIIATQMLH
                     TMIEHPRPTRAEISDIANAIYQRADAIMLSGETAYGAYPVEAVNVMTRVANEVEDVLE
                     TDSSINLARINNEVTAALAKSAVRACSMLPVRAIIVDTLTGRTGRYLSAFRGQIPIYA
                     ICYNEHVMRQLALSYGIEAFYMEPRQSRDHFLSDAISYMLEQRKIASEDMVLIIGGSF
                     GPSNGATFMEIGKVKNLAKKKGEF"
     gene            34621..35517
                     /gene="xerD"
                     /locus_tag="PK839_00635"
     CDS             34621..35517
                     /gene="xerD"
                     /locus_tag="PK839_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HOG71270.1"
                     /translation="MGWSAAIEDYINFLKLEKSLSKNSVAAYHTDMMKLYGFAEIIGV
                     EPESVTGEQIQDFIAHLHDSGLSKRSQARIVSSVRGFYKYLLIEEVVDSDPTELIELP
                     KTGRKLPEVLSVEEIDAMEAAVDLSMPDGHRNKAIIETLYSCGLRVSELVNLRLTDLF
                     FDDGYVRVLGKGNKERLVPIGRKAIADINMYLSQRIMVSITVDAKSGNIVFLNRRGKQ
                     LTREMVFLIIKKYAKAAGITRNISPHTLRHSFATHLVEGGADLRAVQEMLGHESILTT
                     EIYTHLDKEFLRETILKFHPRG"
     gene            35549..35728
                     /locus_tag="PK839_00640"
     CDS             35549..35728
                     /locus_tag="PK839_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009132993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOG71271.1"
                     /translation="MDTGEKIVKTLSSAGKPMKAGEIAEAAGIDKKEVDKVIKKLVAD
                     GKLHSPQRCFYDIKK"
     gene            complement(35878..36645)
                     /locus_tag="PK839_00645"
     CDS             complement(35878..36645)
                     /locus_tag="PK839_00645"
                     /inference="COORDINATES: protein motif:HMM:NF007757.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOG71272.1"
                     /translation="MKISIIQASPFWESIEENLQLFTAKIESTSTDTNLILLPEMFTT
                     GFTMKPRECDASEAERTLDWMLQTSMVRKAAIVGSIIARKENKLVNRMAFVMPNGQVV
                     FYDKRHLFRMAGEHNQYSAGNQRVVVSYMGWRILLTICYDLRFPVWCRNRNDYDLMLN
                     VANFPDERSYAWNSLLVARAIENQCFVAACNRVGIDGSGANYSGDSQVVDPMGQIVAK
                     ANHNSESTIEASISLEQLHEIRASFPAHLDADRFEIL"
     gene            complement(36819..37673)
                     /locus_tag="PK839_00650"
     CDS             complement(36819..37673)
                     /locus_tag="PK839_00650"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HOG71273.1"
                     /translation="MNFPADLTERFFVHNGQLLPTTNFTDSMVEGETVVYEVVRVAGS
                     TPIFAIEHLLRLQASIKNSNLPALANNSAASSLRMLTEKNPVAEKNVRITYSVSTNHP
                     VRNLTVYYIPSRYPTRIEKQKGVVLECIDAERPNPTVKVENKSLRELTNEIIESHGCY
                     EVLLVNAQGEITEGSRSNVFFISRDRLFTAPETKVLSGITRQKVVQICHSLNVELVER
                     CIGIKELAFMDGCFLTGTSPGVLPVRQVGNVSMNASIPVINAISMEYERLVEKSKAEW
                     HSFTGSSK"
     gene            complement(38028..39173)
                     /locus_tag="PK839_00655"
     CDS             complement(38028..39173)
                     /locus_tag="PK839_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG71274.1"
                     /translation="MKKIYKGVMVAISVIGLVAILLFIFWQLQPSKKLNIYILDKTVT
                     RSNRPEHRALVWVLNQDRIVGPDGKTYRVNEDYWGFFPIDLNQQLFDFKAIRLNEVDA
                     YAAAYDAAYYADCYGVYSFEWYKNDAQPIRSSKVYGGLNQNDYLLLKSMKDMGKLIIG
                     EYNMFSTPTNALMRSKAEELFNVSWTGWSGVFCRNLTPNEAGGPAPWMPKLYESQHLK
                     PWPSEGKGIILLSNDGLVDVLVDGEDLNSAFPVIISDNTGISRFGLISEIPFAGWFEF
                     VVPGSNAVTPSTFKINTTQQGLQKLRAIGLTNEFPAVIQAKSNEFTFYFAGDFTENKV
                     TMFTSKMMGGKFLNQLLEGKSENSQFFYKFYSPLMENILNSYINKTQ"
     gene            complement(39592..41598)
                     /locus_tag="PK839_00660"
     CDS             complement(39592..41598)
                     /locus_tag="PK839_00660"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOG71275.1"
                     /translation="MFDKPNKLANEVSPYLLQHSLNPVSWYPWGNEAFEVAKRENKLV
                     VISIGYSSCHWCHIMEKESFTDEQVARLMNEKYVSIKVDREERPDIDHIYMSAVQIIT
                     RRGGWPLNCIALPDGRPVYGGTYFPKDQWLNILESMDATWKSEPERVYEVAEDLAEGI
                     ANTEIIHVKTKLTELDFTGIVMGNLQSIEPALDYGLGGTRGAPKFPMPGLLGYLMQSA
                     QATGNLKLKSFVSTTLDNIANGGVYDHVGGGFFRYAVDSRWHTPHFEKMLYDNAQLVE
                     LYSLAYRINPNPSYLNIVFNTVDFLDRELHSPDGGFYCSLDADANGLEGGFYTWTKQE
                     LDEVLEFDSEIFCSAYGVTPSGDINGRNGLKRCASDDQLSSLFDIPPQEIQFRLQRSL
                     KTLASIRNKRIPPLIDDKVLASWNGLLVSALAQAFITFKNPRFIGLASEIASYLETKH
                     FVEGNLKRVFCKGKTYGNALLDDYANYIRALIMLYQATLEEKWLFKANSLAQEAIRDF
                     WDEGKGMFYYTPGSSDLMVRKMELIDGVIPSSSSIMTGNLLFLAKHIENPLYSKIAMQ
                     VLANLAENTKNNGVFLYGWGNLMLSLALPEVSITIFEGTSNDVNDILGRVIYPNIYFE
                     LIAKKVDHKFQLCVGSSCRLPMDSAADVAQAVNGIGLHNNSSNF"
     gene            41777..43189
                     /locus_tag="PK839_00665"
     CDS             41777..43189
                     /locus_tag="PK839_00665"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HOG71276.1"
                     /translation="MKNLLFALSFFFLSFGVFAQNHNGYRIEIKIDGLKDTTLLLGYH
                     FGDKKFVSDTARIDSNGVAIFEGDSLLKGGIYLVILPDKTYFEVLLSNNQRFGVQTTA
                     KNMVENLKFTGSKENEEFIHFQQFMMGVQAQSNELSAKMKDALTDSTRSNQLRKEMNE
                     LDEKVKAYWLEVENNNRGTFLASIIKAMRSVEVPEFTIPANTPAYDSLKWVHSYNFNR
                     DHYFDNIDFSDSRLLRTPILENKINIYFDRVLIPHPDSIAAAAVNLVEKSKANREMFQ
                     YILSYLINKFQTSERMGMDGVFVVLAEKYYLGGQAWWADQKLLDKISERVNALKPNLI
                     GKVAPDLWLPDPNGKYFRLSNINSKITVIFFWDPECSHCKKEMPKLKNVYDNYKKYGL
                     EVYAVYTQGDQPKWMESISKNDLTWINVWDPTFSSNFRNLYDIYSTPVIYVLDSGKKI
                     IAKRISVETLESILKQELKL"
     gene            43473..44951
                     /locus_tag="PK839_00670"
     CDS             43473..44951
                     /locus_tag="PK839_00670"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HOG71277.1"
                     /translation="MESLLTNTYFWVVLAYFAVVLFISFFTKKLASRSSADFLVAGRN
                     LGLLVCSMVVAAEWLGGLSTVGVSEKAFDTLTLQPILYNFSTAIGMVILGFTLAKAYR
                     ERKVNTVSEMVESIFGKRARSISAIAFLIAFITLAYVELTTFATVLSPIFNINWVWAV
                     IIAATITTIYTYMGGMHALTIVAVFNLVVRYVGLGIALIIGLVAIGDFSNLTNRLVEI
                     GAPQNLYNPFSIGWNAALSLILGGIFGGMASQASIQPIFAAKNPITAKRAAILSAVFI
                     APFGIITGILGLIARSGLFFEVTPGFNPKMALPRLLMTPEFVHPVIGGIAIAAILAAI
                     LSTIGPVSFSIVTIATKDIYHGLINKKASDEKILQFAKRLVILVNLVTVPLALFMKGA
                     ILDAGYFSYAIRTIGAIVILLGIYKLKWISTLSVQLSFIGGTLAIIISIVAKKFGWFD
                     IDKTYASIIATVFFIIIGNIYTKYFTGKQKSSELNINESATK"
     gene            44932..46143
                     /locus_tag="PK839_00675"
     CDS             44932..46143
                     /locus_tag="PK839_00675"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HOG71278.1"
                     /translation="MNQLLNDIRVLELANVLAGPSVGMALAELGARVVKVENLLTLGD
                     VTRTWKLPSEDPDTNISGYFSCVNWGKKSLAIDLNKPEGLDIVYRLTEVSDIVLVSYK
                     PGDAEKLKVDYNTLKQYNKSLIYAHLTGYGLKNERAGYDAIIQAESGFTYMNGEQGGK
                     PVKMPVALMDILAGHQLKEAILLALLNRFRKGEGTYIEASLIKSGVAALANQATNWLV
                     GNSIPQRMGSDHPNIVPYGRIFYTRDEKPVVLAVGSDKQFAELCSILGKPELAQDPKY
                     STNFNRVKNRNEIYPILQDLVAQIDRDPFLNELARRRIPAGGVYNMQEVFDQPEAQEL
                     VMSGTTSAGTPIKGLRTVAFSYNENSKPLELAAPPYYGEHTIQILKETLGFSNDCIEG
                     LINKNVVYAPR"
     gene            46130..47254
                     /locus_tag="PK839_00680"
     CDS             46130..47254
                     /locus_tag="PK839_00680"
                     /inference="COORDINATES: protein motif:HMM:NF013974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HOG71279.1"
                     /translation="MHPGKTSLAFIDGSRLITESLVRAGADTFIGYPITPANLLYLYA
                     TKRYPTMLAAPDEITTLQWMSGFAIAGHVPVTATSFPGYALMLESINMAAMFELPMVI
                     VLVQRLGPATGTATCGAQGDLSLVHGTLSGGYTLPTFSPSNLNDCWDISAKALHTAIR
                     LRTPVVLLTSKEMVMTLQAFDWSHLAEIETLKVKTFSGSDTYIPYKVDESLVPDFMPV
                     SQNNHAVRLTASTHDTKGFLQNSTPEAIENTKRLEAKVTKHLQEYLLFDLDEQAGAQH
                     LVVSYGISAQASREAVRQLRARGEKVSLLVAKTLLPVSRDYVDIANRYERVVVVEENL
                     TGQLRQLLFGAAGRNGLSGVNAIARMISPSEIVDEVMRKF"
     gene            47342..48610
                     /locus_tag="PK839_00685"
     CDS             47342..48610
                     /locus_tag="PK839_00685"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOG71280.1"
                     /translation="MNNVKFLTGGQLPFCKGCGHVAVAQNTEKALQKLDFNPLDVVLV
                     TDIGCHGIVDKNFLTHNVHGLHGRSSALAAGISAGLSNPNKKVIVFTGDGGATIGMQH
                     LVGGAHLGFDMTVVVHNNMLYGMTGGQPSEFTPCGFKTPTLPEGSTKSGYDICELMLA
                     AGAAYVERVVGIGDFSDSLARAFSTKGFSLVEVMEICTSYGVKSNPGMKLPKLVEEAG
                     WKVKVFTEAKQRLFQTPQNSNPTSLLSEKLEVEPKYSGAISKPVSIMLSGSAGEGVQL
                     AAEFLARAAMLSGLYATKKGSYPVTVGVGYSAAEVIISHEPILYTGSPVPDILAITSA
                     DGLGYARAAAGRMKGGTLYIDQSLDIPQTGAETVVIPFREKVGAQNSSLYSVFYMVGS
                     QHFFPMEALRDIFMANKISQRVSVDIFMQL"
     gene            complement(49187..49861)
                     /locus_tag="PK839_00690"
     CDS             complement(49187..49861)
                     /locus_tag="PK839_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013617848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HOG71281.1"
                     /translation="MGIYLIMIIFFVLSFLISNQLKVRFARYSKIDLPQGMTGKEVAE
                     MMLKQHGIFDVRVISTTGQLTDHYNPTDRTINLSPAVYNGHNIAAAAVAAHECGHAVQ
                     HAQSYAFLQLRSSLVPVVSVASRWVQWILLAGVILLQSMPAILVVGVALLALTTLFSL
                     ITLPVEFDASRRALQWLGSTGILYGQNVAYARDALKWAALTYVIAALGSLATLFYYLG
                     FLNNND"
     gene            complement(49908..50996)
                     /locus_tag="PK839_00695"
     CDS             complement(49908..50996)
                     /locus_tag="PK839_00695"
                     /inference="COORDINATES: protein motif:HMM:NF014653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HOG71282.1"
                     /translation="MIDYLKLKSYISLLFERMGCSEMDAKSVADLLVAAELRGIPSHG
                     VMRVKDYYQMVAAGRINTRPSVKTIHETPSTATVDGDNALGPVAGLYSMRLAIEKAKV
                     AGTGWVATQNSNHYGIAGYYAMMALEQDMIGISMTNANPLVVPTFSTSKLLGTNPIAV
                     AIPALEQPPFVADFATAPIARGKLALMAKKGEKAPIGLVQDGLGKPTDDPDTLLHGGG
                     ILPLGGDYEHGSHKGYCLTSIVDIFSAVLSGANFGPFVPPQVPYLPIPEHSVGKGLGH
                     FFGAIRIDAFRPANEFKLMMDEWIKTFRAAKSGVNQPPVLIPGDPERENERIKSKEGI
                     TILPQVLNDLNEVAEKLAVEPLSVFSSK"
     gene            51424..52707
                     /locus_tag="PK839_00700"
     CDS             51424..52707
                     /locus_tag="PK839_00700"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HOG71283.1"
                     /translation="MTYNEALDYMVSQLPMFHRVGKAAYRADLGNALVLDEFFGHPHQ
                     KFASIHIAGTNGKGSTSHMLASVLQQAGYKVGLYTSPHLKDFRERIRINGEMISQVEV
                     TRFVEEYRALFDRVKPSFFEMTVAMAFDYFARRNVEVAVVEVGLGGRLDSTNIITPIL
                     SVITSIGLDHTDLLGSTLPQIAGEKAGIIKRGIPVVVSQLQPEIADVFTSKAGEMQSP
                     LVFADSEYKVREMSYSASGTQTFLVDHGGNPEQWDTDLMGSYQRLNLPGVLAALDVLR
                     NEGYRITSDAQKQGLRMVQQLTGLMGRWQKLQDRPLIYCDTGHNVDGITMVMEQIHRM
                     SYAKLHVVLGMVADKDIDGMLSLLPADAIYYFTRAALPRALDQNILKTKAKGFGLKGE
                     AYPTVAQALEAAKGSANEDDLIYVGGSTFVVAEVV"
     gene            52800..52874
                     /locus_tag="PK839_00705"
     tRNA            52800..52874
                     /locus_tag="PK839_00705"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:52835..52837,aa:Val,seq:tac)
     gene            complement(52960..54249)
                     /gene="murF"
                     /locus_tag="PK839_00710"
     CDS             complement(52960..54249)
                     /gene="murF"
                     /locus_tag="PK839_00710"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009016809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HOG71284.1"
                     /translation="MLEELYHHYLNHGSVATDSRSIPKGAIFFGLKGDKFDGNKFAEQ
                     ALNNGASLAVIDNPTFMTHDCILVENTLKTLQELANHHRKQLKAKIIGITGTNGKTTT
                     KELIQCVLSKKYHAVATKGNLNNQIGVPLTLLSLTADVEVAIVEMGASHPGDIAELAS
                     IAAPDIGIITNIGKAHLEGFGSYEGVVRTKGELYDYIARHKGLIIYNQHNEVLKQLAM
                     GIGIANKLEYSNYCKSIKVTNQHDTPFISVNVEMAGTDKPMDIKTKLVGSYNIENIEA
                     AIAVGLHFGVKPELIKNAIEEYNPSNSRSQLIQTKRNTVIMDAYNANPTSMEAAVRNF
                     TSLESPNKIIILGEMLELGSYAESEHKKVVKLLESLGATQVFLIGQNFSGINSSYGWF
                     ADAKACAEYFLSHEVANATILLKGSRGVGLERVLDVL"
     gene            complement(54495..56006)
                     /gene="gldJ"
                     /locus_tag="PK839_00715"
     CDS             complement(54495..56006)
                     /gene="gldJ"
                     /locus_tag="PK839_00715"
                     /inference="COORDINATES: protein motif:HMM:TIGR03524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldJ"
                     /protein_id="HOG71285.1"
                     /translation="MNLKNKPLSLILVAMLGLTSCGISGKGGSNVSSKTGWAYNSPDE
                     GGFEVVTDWKPEAGPGLVFIEGGTYIMGRVEQDLMYDWNAKPRRVTVTSFYMDETEVS
                     NVDWREYIYWLKRVYSDYPQVIKQALPDTLVWRSPLGYNEPFVTDYFRHPAYNDYPVV
                     GVNWLQANDYCQWRTDRVNEMLLVQKGILEIELGQKGDDVFNTEAYLAGQYVGKVKEN
                     LPSLNPEQPEGRPVRFEDGILLPKYRLPTEAEWEFAASGLVGNTYEERMVERRIYPWN
                     GHNVRNSESKNRGQMMANFVRGRGDYMGLAGNLNDQNTFPGPVKSYWPNDYGLYNMAG
                     NVNEWVLDVYRPLSYEDFDEIRPFRGNVFKTPYDKEGNFAKKDSLGRIPYREVTEEEA
                     ANRRNYNKAYYINYKDGDVASTLNYVEGETLKTKDSNAMYDQGKPDAKGAGMTSLIND
                     HVRVYKGGSWKDRAYWLSPATRRYLDEASSTNDIGFRCAMEAVGPQSARQRGE"
     gene            complement(56086..57066)
                     /locus_tag="PK839_00720"
     CDS             complement(56086..57066)
                     /locus_tag="PK839_00720"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HOG71286.1"
                     /translation="MFRKFFVAIGITMVTSIRVFGQDPIFSQLFFNPLYLNPAYAGAS
                     KYMRLGVVYRNQWTMIDMPYSTYGVSFDRTIQSIKSGVGLNIVADVEGKGVFSRTTVD
                     LVYAYGIQPSYNSHLRFGIQTSGIVRTRNYSGLVFPDMIDINGDVTGTYNTSGITTWN
                     YDFSLGVAGDFENFYGGIAVHHLLEPVEATYQDTKAYIPRKYTVHIGADFNLYKWYKF
                     KEVLLLSPNIIYIQQAKFNQLNIGFYLSRHWLVGGFWLRENLSLNSHSFIFTIGYTDD
                     LFRMGYSYDFSIMKYGLRGLPTSSHEVTLGWNFEYKKGKSKYKYIKCPKF"
     gene            57200..61006
                     /gene="porU"
                     /locus_tag="PK839_00725"
     CDS             57200..61006
                     /gene="porU"
                     /locus_tag="PK839_00725"
                     /inference="COORDINATES: protein motif:HMM:NF033707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system sortase PorU"
                     /protein_id="HOG71287.1"
                     /translation="MFRRTFFLSLLCISNGIFAQDTLERAICWNYRQVPTAQAEQTIL
                     WFDNAVYTNPQTMIPSYFELIPIENNGAYNSTPDVLVADEQWVLVANDEMNLLQGVNN
                     FINESVDCRTAIQRGRMMIQLTVPAVRLNSDNKFEKLVSFKIILKRAEKTNDERSYAP
                     ARLQVKSSVLSSGSWVRVSVREGGVYKISYNDLQSYGLSNLENVSLWGNGGHCLPYMN
                     SEQAPDDLNPIPILMSKGDDGIFNQGDYILFYAEGPKTISYNATMDMWEEKSHPYAQQ
                     ISYFITTSLPQSTIETIEESAAPLNRTANSYDAFLTIERNDTNMVKSGREWFGDVFDL
                     TTSRTYTTDLSLPVVGLPLKVWLRAAARSSVASNFTVKANTTTLGTLPLNSVVIGDEY
                     ADVVAVNEQVYTSALNEGNLTIELTYSKPNSTATGLLDFITINTRQQLKYNGSQLIFR
                     DKQSVGSGNITEFSILNSNQNVQVWDITSFNHPYRLNSRYSGGVVAFKQATDTLRHFI
                     AFEPEKAKTVTFVGAVANQNIHGAGQPDFVIVAHPLFMPQAQELAQLHRSLDGLDVVV
                     FSTDEVYNEFSSGNPDLSAIRNMMRYFYRCAATTEQQPKYLLLFGDGSYDNLSDRNGN
                     TNYILTYQSPRSINKVGSFQSDDYFGLLDDEEGEAVGLLDVGVGRLPVSTTEQANAVL
                     AKIQHYVSGSNTGDWQSKLCFIGDDEDGNIHMTDANTLADYLSTSNPEYNIQKIFFDA
                     YRQESSSGGASYPEVTNAINTTVNNGVFLVNYTGHGNERWLAHEKVLMLSDVSSWKNT
                     KQLPLFVTATCEFSRFDDYHLTSTGEQILLSPDGGGIALVSTTRLVYSSPNFTLNYKF
                     INNLFKFNEDSTSHYRLGDLIKISKNLSGTGNNKRNFTLLGDPALMLRYPELGAEITS
                     INGKPVDEEADTLKALSVANLSGRIFTPIGQVASDFSGNATITVFDKKSEIKTLANDG
                     GMPMTFFSWDNVLYKGRAIVNNGLFSIDFVVPKDIRLSFGTGRISIFADDLARVAMGY
                     NESVIVGGVFNSPYTDNEGPEIDVYLNDTLFNGGGISDSSPKLLIRLRDESGINTSGI
                     GIGHDLTATLTSPGGDEVNVILNMYYQSEPNSYQEGYVNYQLADLSEGRYWLRVKAWD
                     TYNNSSEKIISFVVVGAGNLQLEKFYSYPNPFSEGTSFYFEHNMPEDEFNLTIQIFDV
                     AGRLVSTITQSQAYSGGYRVGPIYWNGTDSRGSRLGRGIYICRLSVKTSSGYAASLQH
                     KLVILR"
     gene            61082..62299
                     /gene="porV"
                     /locus_tag="PK839_00730"
     CDS             61082..62299
                     /gene="porV"
                     /locus_tag="PK839_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007482517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system outer membrane channel
                     protein PorV"
                     /protein_id="HOG71288.1"
                     /translation="MRRILFTLLSFVGFIFFSQVSSAQFDSTVSLTGGLNALKIAVPF
                     LTIAPDSRAGGMGDAGVATSPDINSQHWNPAKFPFIESKKWGVAFSYTPWLRNLVNDI
                     NLTYLTGYYQVDKMQAISSSLLYFAMGEIQFTTDNPNDPPVKFFNPNEFAIDIAYSRK
                     FSDYISGAIAFRYIRSDLSGGYSQPGQASAKAGTSFAADIAMYYQHPVKLSDKFGQMA
                     FGFNISNIGTKLSYSDDPMKDFIPINLRLGGRLTMDIDSYNSFSFTVDANKLLVPTPP
                     VYGVVDEQTVIVKGMNPNVSVVQGMLQSFYDAPGGFKEELNEIYYATGIEYWYARQFA
                     LRAGYFYENENKGNRKYFTVGAGLKYNIFNIDFSYLIPTGGFNSPMANTVRFSLSFDF
                     EATRMRGQKVNNN"
     gene            62303..62782
                     /gene="ispF"
                     /locus_tag="PK839_00735"
     CDS             62303..62782
                     /gene="ispF"
                     /locus_tag="PK839_00735"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008764145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HOG71289.1"
                     /translation="MKVRVGFGYDVHKLESNTPFHLGGIFIEHSKGAVGHSDADVLIH
                     AICDAMLGAANLRDIGYHFSDTNPNLKGIDSKILLAETLKLLDSKGFRVVNIDSVVCL
                     QRPKLMDIIPNMQLTLASVLKMDMEDISIKATTTEKLGFVGNEEGVTAYAVVLIQKD"
     gene            62788..63177
                     /locus_tag="PK839_00740"
     CDS             62788..63177
                     /locus_tag="PK839_00740"
                     /inference="COORDINATES: protein motif:HMM:NF024773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HOG71290.1"
                     /translation="MVPENAETFSTLVLGASCKAGRFSYLAIKSLVKHGVRVYAIGSR
                     PCFVDGVQVHAEKINLTNLHTVTLYLNSQNQTTYYDYILSLKPKRIIFNPGAENPELV
                     RLAHENRIDYVFGCTLVMLNDGSFFKS"
     gene            63424..64017
                     /locus_tag="PK839_00745"
     CDS             63424..64017
                     /locus_tag="PK839_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG71291.1"
                     /translation="MKKTVLLGFAALSMVAVLSGCGKVPQEKIDAANAAIDSAKMVEA
                     DVYVAADFAAVQDSMSAVMADIEVQKSKLFKKFGPANEKLDRILSQAHQIQANAVTKK
                     QEVKVEVEGLMTEVTTLIDQDKTLMKKAPRGKEGAAVLEQIKTEIAAIEASVAEAKTM
                     YDNGKYMDASNKLKAAKDSATKINTELTDAIAKVKKR"
     gene            64304..65449
                     /locus_tag="PK839_00750"
     CDS             64304..65449
                     /locus_tag="PK839_00750"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HOG71292.1"
                     /translation="MSRKSKRIAVIILASLVVATGISVLFYKLIPEPPIGDVDYARLC
                     LQQASKSKAQSYSKKSFKQAKADYDSAMKCWQKENEKFILFRDYSMVAKLAQKSAKEA
                     RNAMNNSQNLRKNMVVAVKDKIELLNGLILNVNELFNRVPLPQEVRDKISRGKLLLKE
                     AQLYFERGEYLSAQDKIVLAEDMLMSSYNRTLDDLEEYFNSQPYWRKLVNSTIENSAK
                     TNGNAIIIDKFARKLYIYQSGVKKLEFEAEFGKNWMGRKRLKGDKATPEGIYLVKTKI
                     PQNKTKYYRALLLNYPNNDDVEMFRREKQQGKLPANAQIGGLIEIHGNGGKGTDWTDG
                     CIALSDNDMLKVYNFIQVGTPVVIVGSLADFKSITENFKLRKHTERS"
     gene            65456..66181
                     /locus_tag="PK839_00755"
     CDS             65456..66181
                     /locus_tag="PK839_00755"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HOG71293.1"
                     /translation="MDAVNIALATTDKDKSIGKVSKFNNILAGLLIVILSIILFFAIL
                     IYAIPPLTELVMKVNGFAPPSSEIFSPTPKDYAKIKLLTGKMQQKYVALTPPRPYIVV
                     NTTYNRFKLYKNRRLIREGFCSTGSYTLLKSHFNREWIFQTPKGFYRIQGKITNPVWR
                     RPDWSFVEEGLPIPSQNDPSRWEAGVLGDYAMSIGDGYLIHGTIYKRFLGMPVTHGCI
                     RMNDDDLEAVYKTLELGSKVYIY"
     gene            66193..66978
                     /locus_tag="PK839_00760"
     CDS             66193..66978
                     /locus_tag="PK839_00760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG71294.1"
                     /translation="MQKKFLKIFLLACLPIFALALVYFILKLVAVNGAVDRFQKFYSQ
                     LDGDDKSNAVFQLTNDSAYNAEQNSYAYYHAQLEAANSDSVVLCINFPDSTATLLIKG
                     VTVHTAKIDRYRVSNFFTKIDQRILYKALSYPFRVERQTSSIPKVPLMIKIAPKDTSE
                     YTPDIVPDTTDVNPVYFLLEAKPGMLVYFYETPKNQSRISFNGVITDIKDRTAILLDD
                     LQRIVHFRKLDYKPSITLRLNRKDAKIIFRAIPNDALIILKLS"
     gene            complement(67107..67184)
                     /locus_tag="PK839_00765"
     tRNA            complement(67107..67184)
                     /locus_tag="PK839_00765"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(67147..67149),aa:Pro,seq:cgg)
     gene            67369..68649
                     /locus_tag="PK839_00770"
     CDS             67369..68649
                     /locus_tag="PK839_00770"
                     /inference="COORDINATES: protein motif:HMM:NF022466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HOG71295.1"
                     /translation="MVESFIHFLWQYKSFAFSGLNTSDNCQLEVLDPGTYNSDAGPDF
                     FNAKVRLNGTTWVGNVEMHINSSEWFAHNHHTDRAYDNVVLHVVVNNDKPAINSRGVP
                     IPTLAISYPFGLEREYSRLTSSGSYIPCGEVLPNVDNLTKRALLDRMMVERLQAKTDE
                     IGKSLEKCHGSWEEAFYRAVTRTFGLKVNAFPAEILAQETPLKVLAKHKNNLFQLESL
                     IFGQAGLLNRFDVEDEYLLSLRMEYSFLKKKFNLTPIDGSLWKFLRMRPVSFPTIRLA
                     QLAMLIHHSTSLFSKAVEASSLKQLQELFQVETSPYWYNHYLFGFPVKSRKKSLGTAT
                     INVIIINAVIPFMFAYGGWRGNQKLKEKAISILEEIPPEENHIIKKFDSMGVKSCNSS
                     DSQAVIHLKSTFCDARKCIFCPVGIKLLLKAAYI"
     gene            68755..69657
                     /locus_tag="PK839_00775"
     CDS             68755..69657
                     /locus_tag="PK839_00775"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOG71296.1"
                     /translation="MRILVTGANGLVGKKITRQLLSGGKHEVYATSLKKLQIPGAVTF
                     TSNLLNADINFMVDETHPDVIIHCAALSSPDACEVDRFACKRLNVDLTARIAMACNDY
                     GVQLILMSTDFVFDGLKGQYAEEDRANPICYYGMSKAEAEQVVASMSAPWAIARTSLV
                     FGYEQRLSRSNIVLRVAENLIKGNPFRVAFDQIRTPTLAEDLARGIEAIATNRATGIF
                     HLAGSEIISVADLAARTAEVFGLNPELLLPVSTEMLTEPARRPLNTSLCCHKAIEQLG
                     YSPKGLTEALRLVYSQMKLDGYNP"
     gene            70065..71327
                     /locus_tag="PK839_00780"
     CDS             70065..71327
                     /locus_tag="PK839_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HOG71297.1"
                     /translation="MKKISVNIQSEIGELEGVIIHTPGPEVENMTPENAERALYSDIL
                     NLSVARKEHAQLSGVLSKVTQTFEVGDLLEQVLSNPSAKEELIRRICLTENALWLMDD
                     LLAVSPKELGKFLIEGVPLVRNNLTNFLSHERFSLKPLYNLYFTRDASFAISRSVVIA
                     KMASTVRHRESIIMEAIFSMSDIFDTSTVNPSAFTLASNATIEGGDVLVVRDDVLLIG
                     NGARTNTHGIDFILNRLQLNSDARKKYILVQELPYKPESFIHLDMVFTLLDADKCMVY
                     DPIIIQPNRYQTVQITIANGRVEEIKTVDNLLVALKKLGMDLKPVYCGGTTDRWVQER
                     EQWHSGANFVAIGPGKVIGYARNVHTIEEMNKNGFEVIRAKDVIAGRVDLGSYSSYVV
                     TIDGSELPRGGGGARCMTMPVRRKKVNW"
     gene            complement(71336..72535)
                     /gene="sucC"
                     /locus_tag="PK839_00785"
     CDS             complement(71336..72535)
                     /gene="sucC"
                     /locus_tag="PK839_00785"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF001913.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HOG71298.1"
                     /translation="MNLHEYQSKALLKKSGVNVPLGSVAFTPDEAVKVAESVIEATGT
                     DVIAVKAQIHAGGRGKGGGVKIAKSTEEVRRYAHDIIGMRLVTHQTGPKGKLVGKVLV
                     EQGIYQPGPAEVKEFYISILLDRSTSRNMVVYSPQGGMDIEAVASQSPQLIFKEEINP
                     LGLQPYQARKIAFNLGLTGKAYSQMVKFLANLYNTYEANDFSLLEINPVVKTSDDDIY
                     AADAKCTVDDSALFRHPDIAGQRDLDEEEPAEVEAGKNNLNFVKLDGNVGCMVNGAGL
                     AMATMDIIKLSGGEPANFLDVGGSANAKTVAAGFRIILKDPNVRVILINIFGGIVRCD
                     RVAQGVVEAYREIGNIPVPVIVRLQGTNALEGRKLIDQSGLRVYTATSFQEAANLVKE
                     MVEKQAF"
     gene            72611..73153
                     /locus_tag="PK839_00790"
     CDS             72611..73153
                     /locus_tag="PK839_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOG71299.1"
                     /translation="MAKNRKLSNEELHRKTVDEYKMVEKFPIVVVLDNVRSLNNIGSV
                     FRTSDALMVERVALCGICGKPPHPEIHRTALGAENSVLWEYFDKTEMAIEKLRGEGYR
                     IISFEQTEHSVNLVDYMPMPGVKYALVFGNELRGVGQDIVDLSDECVEIPQFGTKHSF
                     NISITAAIVLWDFFAKLKKH"
     gene            complement(73237..74940)
                     /locus_tag="PK839_00795"
     CDS             complement(73237..74940)
                     /locus_tag="PK839_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG71300.1"
                     /translation="MMKGLKFAIVALAGVVLAGCGGAEKMKELPQGTSFKVTPNPLEV
                     HANKVKATIEGTVPAKYFNKKATLEITPVLVYNGGELALPSKILQGEDVQANNQVINY
                     EAGGSWKHDVEFDYKDEMFRAEMELRFVITYKDKQIPFDAPQKIGIGTMATYKLVDLD
                     PKPVMLKDKFVRVTPEEKEAQINFVIQKSDIRKSELTKDEVKMLNDFLLDAAKTSNKQ
                     LKEIKVSAYASPDGPETLNDKLSKERGKNTDSYLADVFKKGKVTNPATVSVETTSEDW
                     EGFQKQVQASNIEDKDLILRVLSMYSDPEVREKEIKNLSKVYKVLAEKVLPELRRSRM
                     IAKVDKVGYSDDTLKYMVENNAYDSLNVEEMLYAATLVDNVDKKIEVYMKAGEKFNCA
                     RGYNNAAYYYILNKNTAAAKAALDKIPADQKIPAVTNNRGCIALMEGNYEAAEKLFAD
                     AADAGNEVKYNLGIIAILKGQYPAAAEYLGGTDSFNQGLALLLSGKVDAAKNTFAKVE
                     TAKGYYGRAIVGARMQEEATTLDNLRTAIGKDASLKARAKDDVEFRNFFQNEAFKAIA
                     E"
     gene            complement(75050..75679)
                     /locus_tag="PK839_00800"
     CDS             complement(75050..75679)
                     /locus_tag="PK839_00800"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HOG71301.1"
                     /translation="MQYLVIEGNIGAGKTTLATMLANDLNANLITEQFADNPFLPKFY
                     ENPERYSFALELSFLAERYRQLNAELRMGSLFHSLTIADYYFMKSLIFAQNTLSGDEL
                     QLYKQIFQIIYNTLPKPNLYVYLHLPVQMLLRNIKKRGREYEQSITGEYLESIAQGYF
                     DFFKQHPDYTFLIIETSKLDFVANQHDFNKIKSAIFDKTYPNGVNRLTF"
     gene            complement(75854..77926)
                     /locus_tag="PK839_00805"
     CDS             complement(75854..77926)
                     /locus_tag="PK839_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007667840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOG71302.1"
                     /translation="MLFYACQKNRIQYPETKRVDVVDNYFGTKVPDPYRWLEDENSPE
                     VAEWVSKQNELTSSYLAKIPFREKIRERLTGIWNYTTMGVPFKEGGRYFFFKKDGRQN
                     QSVLYVMETLNSEPRVLLDPNTFSADGTIALSEVSPSRDGKYLAYAVNDGGSDWQKIK
                     VIALPEGKELKDEISWVKFSSIAWYANGFYYSRYDEPKKGEALSTLNQYHKVYYHTMG
                     TDSNEDKLIFANSQHPLRNYSAQTTENEKYLIIYETESTYGNSIYIKNLTDKRGEFSQ
                     LTTGFEYEYSVLENVDDNLYVLTNYKAPKYKLIKININSMDVGNWKDILPERKDVLTS
                     CQLVGGKIIANYVVDAKSKAEVYTLSGEKTDEIHLPTIGTISGFSGSYSDTLAFYSFS
                     SFTVPPAVYKFNVAKNESTEFFKPDIKFNFDDYETKQVFYTSKDGTIVPMFIVHKKGI
                     KLDGSNPTLLYGYGGFNISMMPGFSTSRLFWLENGGVFALANIRGGGEYGENWYRAGT
                     KLNKQNVFDDFIAAAEYLIKEKYTSPKKLVIQGGSNGGLLIGAVINQRPDLFKVAIPE
                     VGVMDMLRFHKFTIGWAWVNDYGSSEDSIQFQNLYSYSPLHNIKPNTKYPAILVTTAD
                     HDDRVVPAHSFKYIATMQEKNPNPQNPALIRIQTRAGHGAGTPTSLIIDEYTDIYAFI
                     LYNLGLSI"
     gene            78788..79252
                     /gene="nuoE"
                     /locus_tag="PK839_00810"
     CDS             78788..79252
                     /gene="nuoE"
                     /locus_tag="PK839_00810"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005722.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HOG71303.1"
                     /translation="MTETVELVKKLADKHGRSRESLIPILQGVVQHERYISSEAMTEI
                     ARELDISAAKVYGTATFYSFLDTQPRGKYVIRVCKTISCAMKGKNPILQELEDILKIK
                     VGETTQNRKFSLLETNCLGWCHKGPAMLVNEEAYTELTPEKVREIINDYMKK"
     gene            79267..80430
                     /locus_tag="PK839_00815"
     CDS             79267..80430
                     /locus_tag="PK839_00815"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HOG71304.1"
                     /translation="MASNFLRRVDLIFDQDCNFKEVLKSAYQHSNDEIINQLLESGLK
                     GRGGAGFPTALKWKFTSEQADPEKYVVCNADEGEPGTFKDREILFKVPLKVFGGMAIC
                     AKVINAKEGFIYLRGEYRFLLSELENQLKLFNEILKDLKIDFKIKIIVGSGAYICGEE
                     SALFESMEGDRGEPRNKPPYPTVSGFRKKPTVINNVETLVYAFMIFRYGARKFKELGT
                     ADSRGSKVFSVSGDTPKPGIYELELGMTVEQFVDEFGDGDTKAVQVGGASGFCVPRKK
                     FKDTIIGFEGVPTGGSMMLFNSSRSMYNVLKNYLDFFSEESCGQCTPCRVGCQQLILG
                     IEAVKKGDRHAAYLDQLQKLAQTMKITAKCGLGQSVANSFFSIVENFKEEMIY"
     gene            80448..82238
                     /locus_tag="PK839_00820"
     CDS             80448..82238
                     /locus_tag="PK839_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005641930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HOG71305.1"
                     /translation="MAKQINLTIDGFPITVEEGTTILQAAKKLNIHIPTLCYHDDLCV
                     AGNCRVCVVEMKGSKTLPASCAMPAAEGMEIFTNTIKVRTARKHIIDLLLSEHNAKCT
                     TCYKNGNCELQNLASEYKISEPMFIDLVPYKNYQMDAYSPSIIKDDSKCIRCQRCVRT
                     CEELQHVSALGVAYKGANMKISTFFENPMFEVVCTNCGQCVNRCPTGALVERNYVEEV
                     WDAIYNPNKHVVVQTAPAVRVALGEALGMKPGQIVTGKMVTALRRLGFDSVLDTDFSA
                     DLTIMEEGFELLGRLKKALVDKDSTVKIPMATSCSPGWIKFIEHTYPEYLDNLSTCKS
                     PQQMFGALAKTYYAKKRNIDPASIVSVSIMPCTAKKFEADRPEMHSSGYKDVDYVLTT
                     RELAIMIKQAGIEFDSLPETNYDSIMGDSTGAAVIFGATGGVMEAALRTAYEVVTGRE
                     VPFDGLNITPVRGMEGVREAAIKIENTKPEWKFLDGVVLKTVVAHGLTNAKKVMDVVK
                     SGKADWHFIEIMACPGGCIGGGGQPIPTNEEIRKKRAEAIYREDANKKIRKSHENPEV
                     IAIYKEFLDEPNSHKAHELLHTHYKERQRY"
     gene            82665..83009
                     /locus_tag="PK839_00825"
     CDS             82665..83009
                     /locus_tag="PK839_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG71306.1"
                     /translation="MRIGELVKHIDGKVLCCEECLDSEVVYAFASDLMSDVLTVKCEN
                     FLLITGLSNIQAVRTAEMSDIQVIVFVRNKRVSEDMIRLARENSIVLVECSCTMFKVS
                     GILYGLGIKAVY"
     gene            83015..83434
                     /locus_tag="PK839_00830"
     CDS             83015..83434
                     /locus_tag="PK839_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG71307.1"
                     /translation="MKFEYKVQGGDFVRAGHASSEVKKILKQLNVEPSLVKRVVVALY
                     EAEVNIVAHAYNGMAYVDIDASRVLVRLDDNGPGISDIELAMQPGFSTASSTVREMGF
                     GAGMGLPNIKSNSDEFSISSEVGKGTHLEFVVYLNSK"
     gene            83453..84826
                     /locus_tag="PK839_00835"
     CDS             83453..84826
                     /locus_tag="PK839_00835"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HOG71308.1"
                     /translation="METSTFHHALKIQSDVCIGCAHCMRVCPTEALRVRGGKALLQSN
                     RCIDCGECYRVCPTGAIIVEQDDFNSIFNYKYRVALVPSVLLAQYPEDVSVDMAIHAL
                     LRIGFTKVVIVEDSVDYLITRINAYIINNPSRKPIISSFCPAIVRLVQVRFPWLVGNI
                     MLVKPPIDLTSSFIRRKLTEEGIPEQEVGIFYITPCAAKIAAVKSPEGEDVSEINGVI
                     NMDLIYNRTLRDIRSGGTKRTVEDGAVNISPKSITWALTGGEALSIEAKGLAIDGISN
                     VIEFLEKMETSEVHGVDFLELRACDESCAGGILTVGNSFLSAERLRKQARYIPESKIG
                     FHTQIKISEYSSVLNGKVGLNRLEPRSIVKLDDSMVKAMNKMERVNRYLCYLPGIDCG
                     VCGTPSCQALAEDIVQGEGSLSQCVFLQKIMEQNKLDPKHSFRIMKKIWGENRFIKDC
                     DKPKHKK"
     gene            84833..85174
                     /locus_tag="PK839_00840"
     CDS             84833..85174
                     /locus_tag="PK839_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine kinase"
                     /protein_id="HOG71309.1"
                     /translation="MTVLDIVKSLGLKVYTETVGMDREITGGYTSDLLSDVMGNAQAG
                     TVWITLQTHINIVAVASLKDIAAILLVKGLEPEPNTLQKSLEEGIPILGTSKQAFEIS
                     GMLYQLLYSNR"
     gene            85171..85911
                     /locus_tag="PK839_00845"
     CDS             85171..85911
                     /locus_tag="PK839_00845"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOG71310.1"
                     /translation="MNLYQADLHIHSVLSPCADLEMSPGNIVSMAKNMGLSIIAITDH
                     NSTLHAPLMRRLAENAGILAIYGAEVTTREEVHCVCLFADEDSRADFQNFLEVHIQKV
                     PNKPDILGYQVVVNEKEEIIEEVEYSLHGALNVGINELEAVVHAMNGIFIPAHIDRPK
                     YSLTSQLGFVPPDLNYDALEVSINSSIESICIHNPSLKRKRFIRSSDAHFIEQVGSVT
                     TTFRMENLNFDGLLKAIKGIDGYDIMLP"
     gene            85957..86505
                     /locus_tag="PK839_00850"
     CDS             85957..86505
                     /locus_tag="PK839_00850"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG71311.1"
                     /translation="MRDLSLHLLDIAQNSIAAQATKIDVHLEINPKLDYLALIISDNG
                     KGMNAEFLQQITSPFTTTRTTRKVGLGLPLLMQNAERTGGYLEITSAVGKGTTLRAVF
                     HPNHIDCPPMGDIAGVAAQLICSFPNISFSFSFKTDTSEFSITTLEIAQALEGIPIND
                     TSVFQLVKQVIESSLEELKFPV"
     gene            86620..87021
                     /locus_tag="PK839_00855"
     CDS             86620..87021
                     /locus_tag="PK839_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HOG71312.1"
                     /translation="MTKVKSLADLKMMKDSLKSKISLREKGENSENLVQIKVAMATCG
                     IASGAKVVMDFLLEELDKRGINSVVTQTGCMGYCYAEPTIEVKRPGQEPVVFGYVDTK
                     KADEIIEKYIKNGELVDGIIPTNYETIDGEK"
     gene            87043..88836
                     /locus_tag="PK839_00860"
     CDS             87043..88836
                     /locus_tag="PK839_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010803102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HOG71313.1"
                     /translation="MAKYKMHLLVCGGTGCRASSSDLIVDRLKAEIASNGLENDVQVI
                     MTGCFGFCEKGPIVKVLPDNTFYTQVKPEDAAEIVREHALKGRKVTRLLYTDPVKKEH
                     VSDSKHMGFYRKQIRIALRNCGFINPENIDEYIARDGYQALGKVLTEMDPQKTIDIIK
                     KSGLRGRGGGGFPTGLKWEIASKNKADQKYVVCNADEGDPGAFMDRSVLEGDPHSVLE
                     AMAICGYSIGASKGLIYIRAEYPLAIKRLKVAIDQAHEYGLLGNDIMGTGFNFDIELR
                     YGAGAFVCGEETALIHSMEGLRGEPTNKPPFPAESGYLGKPTNVNNVETFANVAPIIL
                     KGAEWYTSIGTEKSKGTKVFALAGKINNVGLIEVPMGTTLREIIFEIGGGIRNGKKFK
                     AVQTGGPSGGCLTEKHLDIPIDFDNLVAAGSMMGSGGMIVMDEDDCMVSVAKFYLDFT
                     VDESCGKCTPCRVGNKRLSELLGKITDGNGELEDLERLRNLSNVIKDTALCGLGQTSP
                     NPVLSTLDNFYDEYLSHVVDKKCKAGRCKSLMQYVIDPEACVGCTACARNCPVNAISG
                     ERKKPHEINTAICIKCGACIEKCKFDAISIK"
     gene            88848..90626
                     /locus_tag="PK839_00865"
     CDS             88848..90626
                     /locus_tag="PK839_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HOG71314.1"
                     /translation="METVKLTIDSKQVEVPKGTTIYKAAKSMGIDIPVLCYMDLHDLG
                     VENKPAGCRICVVEIAGRRNLAPSCSTVCMEGMEVKTHTTRVINARRTVMELLLSDHP
                     KDCLTCPKSGRCDLQDMSVRLGIREIPGQEFAAMSTYKKDFSPSIIRDVDKCIMCRRC
                     ETMCNQVQTVGALSAVNRGFMAVVAPAFEQDLVKSPCTYCGQCVAVCPTGALTEVDHT
                     AKVIRALADPTKTVMVQTAPAVRAALGEEFGLEPGTLVTGKMVSALRKIGFNYIFDTD
                     FAADLTIMEEGTELLDRLTRHLNGDKSVKLPILTSCCPAWVNFFEHNYTDMLDVPSSA
                     KSPQQMFGAIAKSYFAEKLGKKREDLVVVSVMPCLAKKYECQRDEFKHEGNPDVDLSL
                     STRELANLIKQFNINFMELPDEDFDRPLGESTGAAVIFGATGGVIEAAVRTAYELHTK
                     KALPRVDFTELRGMDGIRTATIDFDGLPIKIGIAHGLGNARKLLDQVRSGQADFHAIE
                     IMACPGGCIGGGGQPLHHGDSSIIKARMAAIYREDAGKSIRKSHENPYIIQLYEEFLG
                     KPMSEKSHHLLHTQYFDKGKKDIYIK"
     gene            90641..91126
                     /gene="nuoE"
                     /locus_tag="PK839_00870"
     CDS             90641..91126
                     /gene="nuoE"
                     /locus_tag="PK839_00870"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005836594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HOG71315.1"
                     /translation="MSHIKVELKPDQVAKIKEICKSFGNNPGELINVLHQTQGTFGYL
                     PAEVQEVVAAELNIPVAKVYGVVTFYSFFTMIPKGKHPISVCTGTACYVRGAEKVLDE
                     MKRILNVKVGETTPDGKFSLNCLRCVGACGLAPVVLIGEKVYGRVSPDGVKDILKEYE
                     D"
BASE COUNT        24988 a        19534 c        21021 g        26107 t
ORIGIN      
        1 agatcagctt tgagaagttt tatgcctttg attcgaccac gataacgctg ttttcagacg
       61 tcatgaaagg ggttggccgt aaccttggcc gggcgaagtt tatcctccct atctccgttg
      121 cagaataagc aaaacaacag cacagcgaag cctcctgcat acagcgtttt gccgtctact
      181 gctaaaaaat tgcttaacca cttaccagca gctacaagca cgatttcgtg caatcaggag
      241 ccgagttttt ttggcgagtt tgccgaatta aaccgctgca cagctgcaac gtttatatgt
      301 caagctgtcc gggcgcatta tgcatgctcg ttgagggcag aatatgggtg tttcataaat
      361 tttggctata acattttggt tgtattcaaa attcttgtaa ttttgccaaa acctgatgcg
      421 atgttatacc tacctctctg catcctttcc tcggctgtaa tactggttgt tttcaaaatt
      481 gctgaaagga tgaaaatcaa caccctcagc atcattctca tcaactacat cattgcctgc
      541 ctagcaggtt tttttttgtt caggggtgag ggtgcctttt ctgggcaatt tagccctggc
      601 agtattgcct tcatggtttg tctgggagta cttctcgtgg tggtttttag attggtgggc
      661 ttaagcaccc agcgtgcggg aattgcagct acctcggttg cctccaagat gtcggtcgtt
      721 ctccccatcc tgttctctat ctggattgat ccacacgata agcttacctc cacaaagctt
      781 gccggcatag tggttgcgtt ggtagcagtt atcctttctg tttaccgaaa cgataacccc
      841 agggattcac ttcggaaaat ataccttcct gtcctcattt tttttgggat aggaactatt
      901 gactcgtcgg tgaagtatgc ccaggccacc tttgttaccg gcgagctgaa cccgcttttc
      961 aactttgtaa tttttggagt tgcgtttatt gccggtttcg caatggtggc tttttcgaag
     1021 ccagcgctga aagatttgca gcaccttaaa acgtggctag tcggagtagt actgggacta
     1081 gccaactttg gctcgatgta cttcatgatt gctgctctca accacatcaa cccggctaca
     1141 ggcaagcctg cccagggttc ggttgtgttt ggaattaaca atataggcgt ggtggttctc
     1201 ggagtgcttc tgggatatac attctttaag gagcgcccaa cccgtctcaa ctggtcggga
     1261 atcgccctgt cggttgttgc aatttttatt ctaatgaata gctgatgcag gctgacgata
     1321 cttaccaaac tattgagctg aaagccgaag gcctttacaa ggaaaagggt agccggttta
     1381 ttgcgctggc ttttcccgta tcttctgagg tgcaggtaaa ggctattctc gatgacctta
     1441 ggcaggagta ccatgatgcc cgccatcaca gctttgcata catagttggc tataaaggtg
     1501 agaggtggag agctaacgac gatggtgaac cctccgggac tgctggcaag ccaatatacg
     1561 gacagctcct cagttttaac ctgaccaacg tactggtggt ggtggtacga tactttggcg
     1621 ggacaaagct tggcgtttcg ggtctgatta acgcatataa aactgccgcc tgcaacgccc
     1681 tcaacaatgc ccggattgta accaaaacaa tagaccaaac attttcagtt tttgttgact
     1741 accccttggt gggtgaggtg atgagggtta ttaaggattt aagtattcag gtgattgagc
     1801 agcagttcga tacccggtgc atggtgcgcg tatcggtgcg ccaaagcctt tgcggggagt
     1861 ttacctccag aatcaacaag gtggaatcgg catggataga cagcgttgac cagtaattgt
     1921 ttgtaaagcc ctgttaaccg gggctttttt tctacatata acctaaaaac aacatgagca
     1981 tgaaaagtaa aagtttagtt tcaattaacg acctgagtag ggatgaaatg ctcagggtac
     2041 ttgacctggc ggaggaattc gagagaaacc ctagccaacc aattctacag ggcaaagtgg
     2101 ttgctacact tttctttgag ccatctaccc gtacccgtct gagcttcgag agcgcagtta
     2161 acaggttgag cggcagggtt attgggtttt ccgaggcctc gtcgagtagc gtttcaaagg
     2221 gtgaaagcct caaggacacc attctaacag tagccaacta ctccgacctg attgtaatgc
     2281 ggcatccggt ggagggtagc gcccgttatg ccagcgaggt atcgccggtg ccgattatta
     2341 atgcaggcga tggcgccaac cagcacccca cccaaacgct gcttgacctg tactccatcc
     2401 gcaaaacaca gggaaccctc aacgacctaa agattgtgat ggtgggtgac cttaagtatg
     2461 gccgaactgt tcactcacta ctgatggcca tttcgcagtt taacgcctcc tttgttttca
     2521 ttgcccctcc cgagctgcaa atgcctcagg agtacaagct gtttcttaag gaatcgggcc
     2581 tgaagtttga ggagcacacc gacctggccg ataatattgt tgatgccgac attgtgtata
     2641 tgacccgtgt gcagcgcgag cgtttttcgg accccatgga gtacgaaaag gtaaaaaacg
     2701 tttacgtgct tcgcaaccac atgctagcca acaccaagcc caacatgaag gtgatgcatc
     2761 cgctgccgcg ggtaaacgaa attagcattg atgtggacga cagctccaaa gcctactact
     2821 ttacccaggc cctaaacggg gtgtacaccc gaatggccat catgtcgcta attcttggac
     2881 tgaagtagct acgaggtgcc atgacggaat gcatggcgtg gtagtgtata ttttataatt
     2941 cgttgcaatt taaccagcta ataaattaat tcgatgaaag acaaaaaaca tttaagtgta
     3001 agtgcaattg aaaacggaac ggttattgac cacattcccg ctcagaacct atttaaggtt
     3061 atctcaattc ttggacttga cagaatcccc aaccagatta cctttggaac caatctggaa
     3121 agccagcggt tgggtagaaa ggccatagtg aaaattgccg acaagtactt tgaacgcgag
     3181 gagattaacc gaatagccat tgtggcgccc catgccaagc tcaacatcat cagggattat
     3241 gaggtggttg agaaaatgct ggttgaggtt cccgaccata ttacgggcat agttaggtgt
     3301 gtgaacccta aatgcattac caactttgag aatgttgccc caaaatttga ggtggttgac
     3361 aagaaaaatg ttagcctgaa atgccattac tgcgaaaaaa tcaccgatca ggaacatatg
     3421 gaataccgct agggcgggaa ttatagatgg aatactttgg aaggaggttt cgccgctaat
     3481 cgaaggctgt agaaaattat gctgaacttc tggcccgagc agtagtggta gaaagcatca
     3541 tgtagtgcga aacaatatgc caatgaatta aaaattaatt tttaggcaca tactcccaga
     3601 aaaccttgat gtcgtcctga ttcaacctgg cattgagcca gttggaaatc tgcttttttt
     3661 cggcaggggt tatcccggag gttataccga aaaatactac cgtggtttta tcgggttgag
     3721 aactgtcggt tttatactgg aaggtttcgg cataggagca gctggaaatc ttagggaaaa
     3781 aagcttgaat ctccagcagg agggttttgc ctagctgcct tttcctggag atactatcaa
     3841 tttgggcatc tttatccctg ataatcatat ttaagcgggt tagctcagac ctaagcttaa
     3901 taacctcact gtcggtttca tttgagctat tcaaggctaa accttgctca ataagtattt
     3961 tggcatcatc gagatcaaaa ttgctcgcct tttgggtaat cgcttctttt tgcttttcag
     4021 ttaagcttac acctccatag gttagcgtga tgattttttt acgtacatca atgttatttc
     4081 taagtaggta gttgccctca aaggaactta cattcgaaat gtaccggcta gcattcaggt
     4141 taaatttctc attttgcacc agcctgtagc caaaataaat gctcggaagc agcaccacga
     4201 ttattaccgt agaaatccat ctgtttatgc ttttcttttg cctgacatcc atgtcagcaa
     4261 ttatcggaaa tttaagaatt tgagaaatgg caacagatga aatacctata aaaaccgtat
     4321 taatggtgaa gaggtagagc gccccaaaga aaaagttgaa ctgccctgtg gccaatccgt
     4381 agcctgccgt gcaaagagga ggcataagcg ctgtagcaat agcaacaccg gggataacat
     4441 ttcccttttg cttactgcta atggcaacga ttccggccag cccgccaaaa agagcaatta
     4501 ttacgtcata gattgtggga gttgttcggg atagcagctc tgaatgtgca gttgatattg
     4561 gacttattga aaaatagagg gttgaggcaa aaagcccggc aagcacggca aaaccataat
     4621 tttttaaaga aattttaaag agcttcatgt tgtaggttgc tatgctgtag cccataccat
     4681 ttataggccc cattagtggc gatatcaaca tagcgcctat aattaccgct gtagagttca
     4741 tattcaggcc aaccgatgct ataatgatag caaacattaa aatccacagg ttggttcccc
     4801 tgaacaccac gtccttcacg atgctttcat gaatgcgatc atagctgtcg agctcggact
     4861 cgaggttgaa gtagtgaaaa aatttgctga ttacactttg tggtttcatc cgtacaaaat
     4921 ttttaaattt ctgattacaa ataccttgac ccataccttt aaggcatcgg ggttgggcgg
     4981 atggaacacc catattttgc taacgcgtgt ttgagcaagc tacattataa gtatgtttca
     5041 tatcattgaa agcttacttt tacctgtatc ggtgattgcc taacaagctc cccgaaatta
     5101 ataaaaaaat cctaaaggtt gaatgcctgg cataaccctg tcgaagcgta aggaattaaa
     5161 gggaaaagga taaatgttag cacgtgtgct gatgagcatt agactcgatt tctcgatttc
     5221 ttcctgttat tagcaaggta acttcttgat ttttattgtt ccatgtaatt gagaaccctg
     5281 accatgtgaa aggttaattt ttttatccct tatattttga ttctctttgt taaattttgt
     5341 tgaaaaatga atttctgttt ttcaaccttt tttaactttt gctgtcatac taggcaaagg
     5401 tgttaactag taaacttaaa ataaaatcag catcaaatga cagcattttc taaaaggctt
     5461 atcaggatat ctactacggt tgctattgct ctacttttag ttgggattat tctgtatccc
     5521 aaggtaaaag atgcttcaaa tagtgattct aaagataaag taccaggacg aatggccatg
     5581 cggggtgggg gaggccagcc gctttttgcc aacggtttta ttattgttcc aacatccatg
     5641 agcgagttga tctctaccac cggcagctta ctcccggatg aggaggttga cctttcattt
     5701 gaaacatccg ggaaggttgt agccatttac tttgaggagg gaacaaaagt gaaaaagggc
     5761 gatttgttgg ccaagataaa cgatgccccg ctgcaggctc agcttcagaa gcttcaggct
     5821 caacgcaagc tggccgagga aaagctgttc cgccagaagc agctacttga gcgcgatgcc
     5881 atcagtcggg agagctacga ccaggtatct acggagctac aggccattga ggctgatatc
     5941 atgctcatta aagcgcgtat tgccgaaacg gagctacggg caccatttga tggaattgtc
     6001 gggttgcgct tgattagcga gggtgcctac acttcaaccc aaaccccaat agcccatctg
     6061 gttaaggtta gcccccttaa aattgaattt tccattcccg agcggtacgc cggggaggta
     6121 accccgggct atgacattag ctttgttatt gacggaattg aaagtaagtt taccggaaag
     6181 gtttacgcgg tagcacccaa ggttgacatg gctacacggg ccatcgtggt tagggctatg
     6241 tacccaaatc ccaacgaaga gctgaagcct ggtcgttatg ccagcatcac cctgcagctt
     6301 tcaaaaattg aaaacgctgt ttcaatcccc accgaggcgc taatccccga aatggagggt
     6361 gaaaaggtat tcatctacag gaatggaaaa gccgaacagg ttagggttac caccgggcta
     6421 cgcaccgagt cgcgcattca agtgcgcaca ggcctcaact ttggcgacac tctgcttacc
     6481 tccgccattc ttcagctgcg acagggcatt cctgttcagc ttgatacaat tattactaat
     6541 gcaataaatc tccagtaaag cacatgagtc tttcatcatt aagtataaat cgcccggtac
     6601 tttccaccgt attttccctc atcatcctgc tacttggcgc aataggtata acctttctgg
     6661 gtgttaggga atttcctagc gtagacccgc caatcatctc ggtgagcaca tcctaccccg
     6721 gagccaactc cgatgttata gaaacccaga tcaccgaacc cctggagcag tcaatcaacg
     6781 gcattcccgg aattcgcacc cttaccagcc ggagtagtca gggaagcagc aggattaccg
     6841 tggagtttga actttcagtt gacatggaga ctgctgcaaa tgacgtgcgt gataaggttt
     6901 cgcaggcaca gcgtcttttg ccacgcgatt gcgacccgcc aactgtttcg aaggccgatg
     6961 ctgacgcgcg tcccataatg aatattgcca ttaacagcaa gaagcgctcg ctgctcgagc
     7021 tttccgaaat tgccgatctt actctgaaag agcagctgca aaccatttcg ggcgttagct
     7081 cggttggcat ttggggcgag aaaaggtacg ccatgcgtat atggctcgac cctgcgaagc
     7141 tggccgggta ccaaatgact ccgcttgacg ttagaaatgc gctgaatcgt gaaaatgtgg
     7201 agcttcctgc gggcagtatt gaaggggcaa acaccgaact taccattcgg gtgctgggcc
     7261 ttatgtcaac acccgaggaa tttaacaatt taattcttaa acgttctggc gagcagctga
     7321 ttagggttcg tgatatcgga agggctgagc tggcccccga agacctacga agcattatga
     7381 aaatgaacgg tgtgcctatg gtttcggtag ttgttattcc acagcccggg gcaaaccaaa
     7441 ttgaaattgt ggatgaggtt tacaagcgta tcgattttat taaaaaagac ctgcccgatg
     7501 atgtaaatat tgtggtgggt ttcgacaaca cccagtatat aaggtcgtca attaaggagg
     7561 tgcaaaccac catttacatt gcattttttc tcgtggtggt tatcattttt gccttccttc
     7621 gcgattggcg aaccaccctt atccccatcc tggtaatccc cgtttcgctg gttggcgtat
     7681 tcttcataat gtaccttgca gggttcagca tcaacatttt aaccctgctg gcaatagtgc
     7741 tctccattgg tttggtggtt gacgatgcca tcatcatgat ggagaatatc tacgttaaga
     7801 ttgagcaggg tatgaccccg ttggaggcgg gtgttaaggg atcgaacgaa atattttttg
     7861 ccatcattgc cacaaccatt accttgattt ccgtattctt tcccattgtt tttcttgagg
     7921 gtatgacggg gaggctgttt cgggagttta gtattgtaat tgcaggagca gttgccattt
     7981 cggcttttgt tgccctgact ttcaccccca tgctttccac caagctcctt aaaacccggc
     8041 ataccaggag caaggcgtat agctacaccg aaaagttttt tacctcgttg aatagtgggt
     8101 acaggaattc gctaagcagc tttctgaagc ataggtacct gccagttgtt attcttgtgg
     8161 tagctgctgg cctgatagtt ctactttgga aaagtatccc tgccgaaatg gctcctatgg
     8221 aggatagatc gcaggtaagc cttagcgtta cattccccga ggggacaacc tacgactata
     8281 gcgcagcgta tgttgaggat gtggctgctt tggttgataa gctggttcct gaagctgaga
     8341 aggccacttc gcgtgcatgg agaggaggtg cttccatgga tgtagttctg ataccgccat
     8401 cggagagaga ccgctcgcag caggatattg ccaacatgct aaccgctgaa ctcagaaaaa
     8461 agaccaaagc cagggtgaat gtaattcaac agtcaacatt tggtggacag cgggccagct
     8521 tgccaatcca gtacgtgctt caagcccaaa acattgacaa gctgcgtgaa atactccctg
     8581 ctttcatggc cgaggtgcag gataatccaa cttttaccat ggccgatgta aacctgaggt
     8641 ttaccaaacc cgaacttcag gttgaaatca accgtgacaa ggccaacatg cttggggttt
     8701 caacccaaaa tatcggtcaa acccttcaac tcgcccttag cgggcagcgt tttgggtact
     8761 tcatcatgaa cgggaagcaa taccaaattc ttggcgagct gccacgtgct acccgcaatg
     8821 aacccctcga tttgaaatcg ctttacgttc gcagcgatgc aggacaaatg gtacagctcg
     8881 ataattttgt tgaggtgaag gagtcgaccg ctccccctca gctttaccgc tacaacaggt
     8941 ttgtttcggc aaccatatcg gccgggctgg cagagggaaa gaccattagc cagggaattg
     9001 aggagatgga taaaattgca gccaaaactc tcgacgatac ttttagaaca gctctggctg
     9061 gtgactcgaa ggacttcaca gaaagttcat caagccttat gtttgcattt gccatggcca
     9121 ttgtgctcat tttccttgtt ctagctgccc agttcgaaag ctttaaggac cctgtaatcg
     9181 taatgatgac cgttcccctt gccttggccg gagctttaat cttcatgtgg tactttaaca
     9241 tcaccatgaa catattcagc cagattggaa tcatcatgct tattgggttg gtttcaaaaa
     9301 acggaattct tattgttgag ttttcgaatc aacgtaaaaa tgccggcatg agcaagcttg
     9361 atgctgtaaa gtatgctgct gcagcacgtt ttcgtcctat tctgatgacg agcctatcga
     9421 caattttggg aattctaccc ctggcgttag gtctgggcga gggagcgcaa agtcgcgttg
     9481 caatgggaac tgctgtggtt ggaggtctta ccgttgctac cttccttaca ctttacgtta
     9541 tacccggtgt ttacctgctg atttccagcg aatctgctgc aggtaaagcg gcaaactcaa
     9601 aagggcggga agtcgaaggt attgagcagt agccgctaat ttggttaatg ttttgatggg
     9661 gtttccacac ttggacgtag ctacttttat agatttgcat tctgaaacca attgatattc
     9721 aaactgataa atattaaata ctgatgaatg gaaagtttga tttaatgaac caaacaacac
     9781 cctcagcatt ggcaactcca atggccaaaa ggatttttac tattaccctt ctgatatgcc
     9841 tgtgtagcgg cttatccaaa tcacaggagc tattcgattt gagctcatgc attaaggctg
     9901 gcctcgagca aaatttttct atactgatag cccgcaatca ggaaaaaatt gcctcaaata
     9961 actataccgt agggaatgcc ggatacctgc ctacgctcag cggctcggga agatacggag
    10021 gaactatcaa caccactcac cagtcgttta ccgatggatc ggagagcgag gccaatggtg
    10081 tgaacaatac atcgggggtg gttggggttg acctgaacat gaccattttt aacggcttca
    10141 aggtgaaggc aacctaccag aagcttaacg aaatgaagca gctgggcata ctgaacactc
    10201 agctggaaat ggaaaatctt gcagcagaaa ttgcttccga gtatttcttt ttcatccagc
    10261 agctcaacct ttacaataac atggcatacg ccgtggagct ttcgcacgag cgggttagaa
    10321 ttgatgagga gaggtacctg ctgggttctt cttcaaaagt tgacctgctg cagtccatag
    10381 tgtatcttaa ttccgacagc tccagatttg ccaagcagaa ggaggtgctt agagcttcgc
    10441 aggtgcgact tttcaagcta atgggcaacg ttgacttggg atatgacatt aggccaaagg
    10501 atacggtcat ttcaattaat gatgggttgc tttacgatga tttgctggcc tcaaccatgg
    10561 atcaaaacac ctccctgcaa attgcccgaa gaaatacaac aatatcgcag tacgactaca
    10621 aaattatcgc ttcccgttcg tacccctacc ttaaccttac atctggatat gactattcat
    10681 tcaacgatta tgaaaccgga accgtaaaaa gtcagcaaat tcatggctta agctatggtc
    10741 ttactcttgg agttgatatt tttgacggct tcaaccgcaa gcgcgaacgg acaaacgcag
    10801 ctattgaaat agaaaatcgg gagcatgagt atagcgatgt ggtgcaggga gtaaaagccg
    10861 acttgctaac catttactat gcctatcaga acaacctcaa gctgctgaaa cttgaagagc
    10921 aaaacctttc tgtagcccgt gaaaacctgg aaattgccat ggaacgctat aggctgggcg
    10981 atttgtcggg gcttgaacta agggaagtcc agaaaagttt actcgatgcc gaagagaggc
    11041 ttatctccgt taagttccaa gcaaagctag ccgaaatttc tctattccaa atttcaggga
    11101 aaatcatgag ctacctttaa atttcagctc ccagggctgt ggcatgatca aagacgttct
    11161 agcagatcga tcggctttac aggttgaatt ttgagttaac ctgttaatta tcaggatgaa
    11221 ggtttcgtag aatgctgctt ataaagggtg ttagcagtaa cacccatgga aaatacccgg
    11281 taggtatagc tatcctttca gagcatttct tgttttgtag agtctaaggc tattaccatg
    11341 caggggggga gctgctggtt tgctaaaacc taaaaaacag caaaaaaata gcgcctacga
    11401 gtatccggta gtagccaaaa tacttaaagc cgtaattttc tactattttt aagaagactt
    11461 ttacggcaag ccatgcggta aaaaaggcta ccacaaatcc cgttgccagc aaaacaacct
    11521 cgtgagaggt gaagctaatt ggagttttca gcaaatcata cccagaggcg gcaatcatgg
    11581 ttggaacggc aagtagaaac gagaattctg ttgcctgttt tttgtcaagt ccgttaaaaa
    11641 ctcccccaat aattgtggct gctgcccttg acactccagg aatcatggag aagcactgaa
    11701 ttaatcctat gaaaaaagaa tttttatacg aaatatcttc caccacctca actttactct
    11761 tttttgtagt aagcttttgg tcaataagca ccagaacaat tcctccaaca attagtgaaa
    11821 ctgcaactac aatagagttg aatagatatc ctttgataaa gtcgtaggct atatacccca
    11881 ccaccgcagt gggaataaat gctattccaa gctttacata aatagaaaaa ctttgcaaaa
    11941 actttttggc gtaaagcatt acgatggcca ttattgcgcc tagctgaatg aatatttcaa
    12001 aggtttttac aaaatcattt tcctgaattt tcattaatgt tgaagccaga atcatgtggc
    12061 cggttgacga cacgggtaga aactccgtta ttccttcaac aatagctatg ataattgatt
    12121 gtataaaact catcgtcttt caggattggt tgagtgcttt ttacttttag cctgcaaaag
    12181 taggctcatt ttccataatt gaatcctttt accggaaatt ttttgaaaac ggtatcccta
    12241 agggttaatg ccagcagggt gtatttatga tggatatgcc tacatggctg ccatgcctga
    12301 gggcatgatt tttttacccg tttgcttttt tagaaacgga ataaatgata acgcaggaat
    12361 gttctggcta gtggttcccc gtggagtttc tttgcccttt cagtgtttca ggtaatcggc
    12421 aaaaaccttc tcgagtttgt caacttttgg ggttatcacc atccgacagt agggttggtt
    12481 cttgttgttt tcgtagtatt cctgatggta atcttcggcc ttgtagaagt ttttaaacgg
    12541 ttcaatagcc gtaacaacag gtgaattcca tatccttgct ttttccagct cagctttaat
    12601 ttcctccgct attttttttt gctcctggga gtggtaaaaa attaccgagc ggtactgaaa
    12661 tcctacatct gccccttgcc tgttgagggt tgtggggtcg tgggttttaa agtagatttc
    12721 caaaatttta gccaagggta ttacctcagg gtcgaaagta acctgaacaa cctcggtgtg
    12781 accggtatta ccgttgcaga cttccctgta ggttgggttt actgctgagc cacctgagta
    12841 ccccgattcc actttcaaaa ccccatcaat cctgctaaat attgcctcta cacaccaaaa
    12901 acatcctgcc ccgagggtta tagtttcgct agcgcttgct ttcatctctt tgtggttttg
    12961 gttggtttga ctttcacatg ccatggaaaa ttgcgtaatc agcaaccatg atgcaacaat
    13021 ggcaactcgc ttcatcgtgt agtaatttta accagtaaac agctacatgc cttattttgt
    13081 tttgcagcag gttggattgc atagttatgg ccagcagctc gattccctat ttgaggtaaa
    13141 gggggtgagt gaattcgggt tgcaacctac atggcaatca caccggaacc aattagctcc
    13201 tcgttatcgt accatgcagc aaattgtccc gctgtaaccc ctctttgggg gttatcaaaa
    13261 atgatataga ttccattatc cctggaaagc agcgtagctt tctggagtgg ttgccggtag
    13321 cgtattctaa ccatcaacct tgctgtttgt ccgggcctta gcttttggtc gggacggatg
    13381 tagtgaacgt cgttttggct gacaaacaga cctttacggt ataggccggg gtgatcgtca
    13441 cccatacccg tgtaaaccac gttctgcaga acatcagtgg caataacgaa aagaggttct
    13501 gatttccccc ccacatttaa gcctttgcgt tggcctatgg taaagaattg agccccatgg
    13561 tgttgtccaa cgaggttccc gtcggctttg ctgtagaaag tgggcttgca gagtgcttct
    13621 agatcatcag gagaaatcac cggttgggag ctgtaaaatg ccctgggaat ttcaactata
    13681 tcgccctcaa ctgctttaat tttttgctga agaaaaacag gcaggtcaac cttgccaaca
    13741 aagcaaattc cttgagaatc cttacgttcg gcagtagcca aacccagctc agaagcaatt
    13801 tttcgcactt cgggcttgag caggtgtcca atggggaaca atgccctgct gagctgttgc
    13861 tgtgaaagct ggcacaggaa gtagctttgg tccttgttgc tatcggttcc ggtcaatagt
    13921 cggtagtatg tttgcccatt aacctctatc tcctgcttac ggcagtaatg accggtagcc
    13981 acatagtcag cacccagctc gaatgcggct ttcacgaagg cgtcaaattt tatctcccgg
    14041 ttgcatagca catcgggatt gggcgtgcga ccctttgagt attcgttaaa catgtagtca
    14101 acaacacgct ggcgatattc gttgcttaag tcaacaaact caaagggaat tcttagcttg
    14161 cgtgcaacca tttcggcaaa atccctgtcg tcgtcaaaag ggcaattccc tttgagcact
    14221 cctgttgtgt cgtgccagtt tctcatgaac atgcctacca cctcatatcc ttgctcctta
    14281 agtaggtatg ctgctacgct tgaatcaacg cctcccgaaa gtccaacaac tacacgttcc
    14341 atttctgttt ttttcagctg gcaaagttac aaataagtac ggtttggtgg cagtgcccca
    14401 aatggcaatg tataatacta gggcggtttc agggttaagc ggtttggctg tgttggaaaa
    14461 ttttttctga acgggttaat tttatctccc caaatgcctg atgaaggcat cggagtatcc
    14521 ggcattttta aagctagcca ggtcattctg cgcctcttcg ggcgtagagt agctaccggt
    14581 gcagtaacga tatattccat cacatccacg gtaaaccata gtgctaccaa ttacgtttag
    14641 ctggtttaac cgtttgggtt ttgtgagtgc cataagttga atggtgtagc ttggctgctc
    14701 cttgttaatt ctaacatagg cgttggtgta gcctaatgtc tttaccctgg caaggagttc
    14761 ttcggcttgc tggacgctga gagtataacc aacgctgtaa cggtagtagc cttcgggcga
    14821 tatggaaaca aatatgctgt cgagtttaaa gtagtcgtag tccacaggct gcttgcaagc
    14881 cataagctgc acggaatagg tagggtgggc taatgatgta gaggttccag ctgctaatga
    14941 aaggtttgta acggcaacat gaactggcct gggcttttcc atctcacgtt ttttaacttc
    15001 aggcttggga gatgggttaa caggtacact ggcagctgtt gatttttcct gctttggctt
    15061 ctctaccgaa gcaggaacag gcttttcggg cttaggctct tcaaccacta ctggtacggg
    15121 ctttacttcc ttttgaatcg gtatatcatt tgccggttgg gatacctctg caacaggttc
    15181 gggtttaggt tgggaggggt tcagcaatag gctaatctgg tattcgtttt tgtcgggttt
    15241 tgagggaatt tcgagaaggc tcgaagtaga gtcaaattct acacccgaag cagcaacagt
    15301 aaacgttccc ggggataatt tttgtaaaat ttgttttttc ccgttttggt ttacactgct
    15361 aaccggcttg ccatctttcg tatcaatgat gctaacggtg tagcttttat cggctggtgc
    15421 cttgtcggca agcattatgt tgattttaac atcaacctgg ggaatgattt ctaccgagca
    15481 aatatccaga ccgcccagcc cattgggaga aaatgttgaa atatagccgc tggtcaggct
    15541 ggttgggaag tagaaaaggt tgtcatcggg ggtgtttagg gggtacccaa tattcttgac
    15601 gttagagctt ccttccaggt tcacataaaa gatatcgtat ccacccatgc tgttgtgccc
    15661 ctgggagctg aagaacaggt acttctcatc gggggtaagg aatggtgttg cttcatctaa
    15721 aggggtgttg atgttgggac caaggtttac gggttcgccc catttgtcgc cgctaatggt
    15781 taccttgtag atgtcaaatc ctccttgccc cccttttctg tcgctggtga agtatgcggt
    15841 gttgccatcg cgggttacgc aaacatgggt ctcattagcc ttagagttaa ttggttttgc
    15901 agcctttacc gggttcgacc atttattttt ttgtaaaaag ctaactacaa tttctgaatt
    15961 cttcaaatcg ttatctatca ggtagagctc ttcgccattg taagatagga atgaggtggt
    16021 gaggtagctg ctaccaaggt tccgggtaat atcctttggg attgaccatt tacccttatc
    16081 catgaaggag atgaacacct tgttacccga ggtggatcgg ctggtgaagg ccatcgtttt
    16141 tccatcgccc gaaagaacgg catcgaagtt cgactggtcg ttgtttacac cttcctcaag
    16201 tcggtttagg tttagcccaa ttgggctgtc catcatagcc ttggctgttt cacagctctt
    16261 tatgttttgt tcaacctgct gcaaaacata cgcatcattt gggttgacgt actttttata
    16321 ggcttcaaac tgagctttgg ctttatcaaa attattttga tgcatgtagg agatgcctag
    16381 gtggtagaga acttcagggg gtgagttggc ctccttaagc gaacgggcgt cgtacttgtc
    16441 ggataccttt tttgcagctt cctcaaggta ggagagggct tcagccttcc tgtcatctgt
    16501 tttcagcatg cagtatccta ctttaaacat caggttacag ctctcggggt atttttttag
    16561 ggctttaagg taggtatcgg ccgctctgga gtagttacca tcggccatgt aaaattccgc
    16621 atcagtttca agggtgaaca ggagattcca atcctgtgaa aaggatttcg ttgggaagaa
    16681 tatcaaaatt aaaaagtaga ggtaggaaag atgcctaagg ttatacatag gaattatgta
    16741 aaatcaaaat gttaattttt ctacgaaaat atacacaatg ggttgattca tgatcgccgt
    16801 ttttgcttta agctgctagc aaatttgacc aacatcattt tttccttgat gtagatagca
    16861 tggttatatg acaaaagcgc aaggatgaat ttgtgatgaa aggtacaaaa cttttttatt
    16921 ccatcttcaa ttttgtgggg agttgtttta aatttcacct aatctattac caatgttggc
    16981 caaaactcct taccccgtaa gggcttaagc tgtaaaattt tggtttgaag ttgtgtattt
    17041 agcttaaaga aacttcaact ttacctcttt taaaaatttt tcctgaagga ggttggctat
    17101 acgcttaacc acggttcgtc tgatttccaa ctcaaccggt aggttggggt cctggtgggc
    17161 aatattgatg caggtgccta ttacgaagtc aattatatca ctttcgagaa tcagcttaac
    17221 cagctgctcg gccggtccgt tgccaagggt aacggtttcg ttgtaggaat ccagaatggt
    17281 gttcaccttg ctcagggtga gtattccctc ggtaaccagg tcagcaccat ccatctggct
    17341 ggttgggggt aagtcggggt cggtaaactc catgctgtca acgacttcac gtttgagctc
    17401 acgggcaata atatcaactg tagtggcgcc acagattacc tttttaccct cgtagctatt
    17461 gaaaatactt gccagcatgg agtcattttc cttttcaaaa ggggggccgg tgcaaattag
    17521 tagcttacgg ggatgccgga agtatatggt tgcacaggta gtatcatcct ttgggtggta
    17581 gccgtcatta acgtaagcca tgtttaccac cttggcactt agcttacggg ccgatatgtc
    17641 gggctcgttt tttaccattt tttctacaaa ctctcttgcc ccatcactcc agccaaatgg
    17701 ataatcgggt gttcccatgc ccgattggct tacgccatcg gtccatagta ttatcctgtc
    17761 ctccttttgc ggggtaaagc tgcagtacct caactccttt ccggcatttt gctcgctgtt
    17821 gagtattatg cactgccatt cgggtattag cggttcggtg ccccgcatga taaagcattc
    17881 cgggttatca tactcaagaa tccgggtatc gccgttgtgc tcaatgtcca caatggaaaa
    17941 ggtggagtag ctcatttttc tttcggagca aacggggagg gtgttcatga ttatttcagc
    18001 aatagtgttg acttccttat gttcgcgggt gaagttgaat gccattgttg aggtgagtgt
    18061 ggccagcacg ttggccttca cgccatgacc cattccgtcc gaaagcacta ttatggttcg
    18121 ctcttcatcc tgaagacgtt tggagtaaaa cacatcaccg caaattcgct caccatcgtg
    18181 attcttctgc tggcagttga cttctataaa aaatttctcg gacatggggt gaaggctgtt
    18241 tgcttaaacc attattttgt tttgctgttt tctgctttgg caaccccctc gttgtaggtt
    18301 tgaataatcg agttgagcat ttgctcaatt tctgaggcac cttcgcctag taaaaagcca
    18361 atcttttgaa ccattgccag gttcctgtcg atggcttcgg ttacccgctt aacaacctct
    18421 tctttctgca cggccggagc atacatatcc ctgataacag caccaacaat gctgttgggc
    18481 ctgatggtga atacggagat gttcagcagc ttatcgcggt agtgaacatc acggtttaat
    18541 atatcgctat tattttgaag tacgtaggta aacaggttgt gaatattgta gggtaccagg
    18601 ctttttaggt ctgcaccaac aagtccggga acaatctcgt ttatttctag ggcgtcatcg
    18661 ccaagcaaat caataaagct ttggttgctc tgtatgattt ttagcttctt atcaataagc
    18721 acaactgcag caggtagctt cttaagcatg gtggtaaccg tttcggaagc ctcacgagct
    18781 gcttcctgct ctattcgcgc ctttttttcc gattccctca aagcctcctg ggttttagca
    18841 agcttctcgt tagttgaacg aagggattta atatactcct gcctgttttt tagggtgaag
    18901 ttcaagcaca tgtccgtatg ggcaagcccc gatgccactg cagaggcaaa gtcgtagcat
    18961 gaggcatatc cacaagcttc acaacctgtt tcctttccaa caaattcctt gtcaattacc
    19021 tttagcacct cctcaagttt ttgctgtgag gggagtggca gcctttggtc atcaggggtg
    19081 aaatcccttg tatagctatt cttttcattt cgggatatgt tttcctccca ttttttacgg
    19141 ttaaacgtta gcaatctttt gttggcatag ttaaccacca ttgttcggcg aatgaatttt
    19201 tttccaccct tcgatgtacc cggtcccata aggcagccct cgcagtagaa aaggttaaaa
    19261 tgccggttaa tcatttcggc gctggtttcg aattgctcca gggcttgaag tgtgttgttg
    19321 ggcccgttag cgctgataac tgttccggtt agcaagtctt cgctgatgtc aacggcttgc
    19381 agtatgcctc tgccaatggg gtaaagcgat cccttgtagc caattggctc gtcaaactcg
    19441 gagtactcca ggttgctctc gtttatgtta aattcctcga atagctggcg gagctccaca
    19501 aaggtaagca cctcgtcaat ctggcttttc ccgttgtata gctttgcttc ctccttgctt
    19561 tgtatgcagg ggcctatatg aacaatccgg acattacttc catactggtt gtgtacagcc
    19621 tgggcggtag caatgatagg cgaaatgata ggggcaaggt tgttgaccat ttcgggatgg
    19681 tacttttcaa ccattgatac aaccaccggg cacatgctgg tgatgaagta ctttccctta
    19741 aagtcatcga acagcttttt atactccctg gctacaaggt caacaccaaa agtaacttcg
    19801 caaacgtaag taaaccctaa ggtttttatc atctgaacaa actttcggta gtcagttata
    19861 tccacaaatt cacccgaaat gcttggcgag caaatagcag caaccggttc gttgccctta
    19921 agtagctcca gaacgttttt cttggaggaa cggtagctta ccgcattcac cgggcaaata
    19981 ttgaggcagc ttccacaacc cacacacctg ttttggagga ttcgggcaaa gtcctgaccg
    20041 gctttaactt cgatggcctt taccgggcaa acccgcacac aagcataaca gtttatgcac
    20101 gtttcctcac ttatttcgaa gagctgcgac atttgtttta atcgtttgga ttagtactcg
    20161 ctcaactcgc tatttaggat atccagaata ttgtactcgt caacgcctgt aaaaattttc
    20221 ccattaacgg ttactgtagg cccttcagcg catctgccaa agcaatgccc cccgtgaaag
    20281 taaacctttt cccttaggtg atgatcgtct atgtatttct gtatggtctt tagggttgct
    20341 ttatttcccc tcgcaaaaca ggagctaccc aagcatatca cgatttcgtt ttttttctcc
    20401 atcttattgt ctggctgggc tttataatac ctttcccatg ttctcctgcc aatcctttaa
    20461 attgtttcct ctgagcgttt aatgattaac cctcttgctg gcttacttaa aaaaacttct
    20521 gttaattcaa tcacaaattt aagtaaagtt agattatcta aatttctttt attaatttgt
    20581 tcgggaatta acaggtgaaa ataccaactt aaatatctgt tatacaggaa aatagttaat
    20641 tgttattgag ttaacaattt ttaaaaattt cttgatgggt aatgaaaaga agcattgaag
    20701 ctatacttga acagtatgac catgccggaa tggacagctt aatccccatt ttgcaggaaa
    20761 ttcagaataa aaccggatgc ttaaatgagg agtcaattgt catggttggt aagcatttgg
    20821 gcatacccac ttccaaagtt tatggtttgg ctacatttta caaccagttt aaatttgagc
    20881 caaagggtaa gttccatata ttggtatgca atggcacttc gtgcaggctg aacggctcaa
    20941 aatggatcat tgatttcatt gagaagaatt taaaaattaa aaacggagag actagtcgtg
    21001 atggcacatt tagcctggag gtaatttcct gcatgggagc ttgtgagtcg ggaccgctta
    21061 tttcgattaa tgaagagtac tattgcaacc tcacaccggc taagcttggt gaagttcttg
    21121 ccgggttagc cgagtcggag tagtgccatt tggatattca acattttcat ccacaatggg
    21181 ttaagttgtg atgctatatt tgatatactt ggagttttga acatagctgt tattgaaagg
    21241 ttatgaacca cagttacact ttagccgata aaactttttt ttgtgaacag gtgctaccct
    21301 tgcttcatgc cgagctaaat agtgataccc aaaaacactt atcaacatac aggcacgaaa
    21361 gggtaaaaaa aacagtcata tacattggca ttacaacttc atcggttgtg gctggcgccc
    21421 tgaaaactct gaaagcaata gagggctatc ttgaagaaaa ttcagtagat gccataattg
    21481 tacccgttgg cagctatggc ctttgctcct tcgagccaat tgttgaggtt cagctgccgg
    21541 ggaaaaagcg ggtggccttc agaaacgtaa catacgatag agttgaattc ctgctcgatg
    21601 gtgcatttaa cagctttgtt caacccgata cggcactttt tcagcacaat tccgatattc
    21661 ttgaaccgtg gcccgatatt ccagttaagg atgatttccc tttttttgca aaacaggata
    21721 ggctacttct tagaaatatt ggaattataa atcccgaaaa tctactggag tacatctcat
    21781 tcgggggtta ccgggcactt gcctataccc tgcggaatca aacatccgat tccgtttgcc
    21841 gtattgttga gctaagcggg ttgaggggac gtggaggcgg aggctatcct accggattga
    21901 aatggcgtaa aaccctttcg gttgctgcta ccgaaaggat tatgatttgt aatgctgacg
    21961 aaagcgatcc cggcgctttt atggataggc tcttagtgga aagcgatccc tttcgtatca
    22021 ttgaaggttt aacaataagc gcttatgcca ttggtgcaaa caaagcaatt atctacatta
    22081 gaaaccgtaa caagctggcc gttgaccggc ttaccctggc catggagcaa gcttacgatt
    22141 ttggtttgct tgggtacaac attttagata gcggctatag cctcgatatt tcaatttttc
    22201 aaggccctgg agcttacgtt tgcggggagg aaactgctct aatccaaagc ctcgaaggga
    22261 aacgtggtac gccttctatt aaacctccct accctaacga ggtggggtta aatggtaagc
    22321 ctaccgtggt taacaatgtc gaaacccttg ccagtatccc agatattctc cttaatggag
    22381 ctgactggtt tatgaaaata gggacggcca catcaaaagg aactaaaatt ttctctgtaa
    22441 atggtaaggc ttcccttacc tgtgtggtgg aaatatccat gggcaggcca ctctcagcca
    22501 ttgtagagct ggcaggaggg gttaagcccg actgcacgtt aaaggcaatc catctgggag
    22561 gcccatctgg ggttagcgtt acccctgacg agctatacct ccctgttgac tttgatgagc
    22621 tgaaagtcca cggcatacca cttggttcgg ggggagtcca gatactcgac cagtcggttt
    22681 gcatggttga cctgaccaag tttttcatgc acttcatccg gaacgagagc tgcggaaagt
    22741 gtatcccttg cagggagggc tctagccgta tgtaccaaat tttgaatgag atttcccgtc
    22801 gtcccgattc ctccgacaag caccacaccc tcgagcgctt taagggtgta attcagctcg
    22861 aaacgcttgc tgaggttatg cacgatacct ccctttgcgg gctaggtcaa accgcttcca
    22921 agccattgtt aaaagcttta aaggcattta gagacgagtt tgaggagcac atttttgacc
    22981 gtagctgtag ggccggagtt tgccgcgacc ttagaacttt ttacattgat actgagaaat
    23041 gcacgggctg tgccgcctgc gccaaaaagt gtccaactaa cgccattatt ggaactcctc
    23101 gctctcccta ctttattgta gaggataaat gcacgggttg cggcagctgc atggaggttt
    23161 gcaagtttgc atcggttttc tatagataac ctgcattttt ggttaaaatt atgaaatatt
    23221 cccaactaag agcgaaacct aaaggtgcga ggaatgaaga agcgttattg gaagctaagc
    23281 cttttgtttt atttttttga tacagattga ttactgactt gtttgaaata atttaacaca
    23341 tgaatttcaa cattgaggtt aacaaccgtc aaattctagt tcatcgagga gaaaccattt
    23401 taaacgccct aagggggaat gggattaaaa ttccaaccct ttgcaacatg aaggagttgt
    23461 cgcctaccgg tgcctgccgt ctatgcgttg ttgaggttga gggacgtgaa aggctggtta
    23521 cgtcatgttc gcaacttgtt gaggagggca tgcggattaa aacccattca tcaagagtgg
    23581 taaaagcacg taaaacgttg gttgaactgc tacttgccaa ccacccggac gattgccttt
    23641 actgcgaaag aaacggacgc tgcgagctac aatcccttgc cgttgagctg catgttcgcg
    23701 aacggcatgc ccaaaacaaa accttctccc aaaagctcga cctatcaagc cacagcatca
    23761 ttcgtgatcc atccaagtgt gtgctttgtg ggcgttgtgt tcgaatttgc aatgagcttc
    23821 agtctgtatc cacgtttgaa tttgttaagc ggggttgcaa tatgtacata ggcacagcca
    23881 tgtcgcgcga tttaaccttc tcaaactgca ttgcttgtgg gcagtgtgta atgtcatgtc
    23941 ctaccggtgc gttaagcgaa cgctcaaacg ttgatgaggt tatagatgcc cttaacaacc
    24001 ccaaccttat tccggttgtt caggtggccc catctatttc cgtatcgctt gctagcgagc
    24061 tggggctcaa accaggtaaa gatttgaatg gggtgctaaa cgccattcta cgtaaaattg
    24121 gctttagata cgttttcgac agtgctgctg cggccgatat tgcggtgatg gaaacagcta
    24181 ctgaactctt gaagcggagg gaaaatggag agaagcatcc gcttatatcg tcatgctgtc
    24241 cggcatgggt aaagtttatg gagcagtact accccgaaca cctttcatgg ctatccacta
    24301 ctaaatcacc ccagcaggtg atgggggcgc ttattaaatc gtattttgca ccatcctacg
    24361 gcattaatcc ggcaggtatt ttctcggttt cagtaatgcc ctgcctggct aagaaaattg
    24421 aagcccgacg ggaggagatg actaccaagg gtgtttcgga tgttgatacg gttatttcca
    24481 cccgtgagct tgtgcagctg gtagaactgt acggaattga cgtacagaat gttgaatccc
    24541 aacaacccga ttcccccttc gatattcgta gttcggcagg taagcttttc ggcgtttcag
    24601 gaggatcgtc cgaagctctt gcccgcaccc tgcactacat gaatacggac acggagctgg
    24661 acaactttaa aattgcagaa ctacgttcat cttccggcat tagagagttt aaacttacca
    24721 caggtaaaca ggaatttgtg tttgtagtgc tgagcggcat gaaatccatt catagcttta
    24781 tggctgagct tatcaatgga aatgttaggg ccgattatgt tgaggtaatg gcctgtcctg
    24841 gaggatgtgt taatggtggc gggcagcctt ttgcaatgga ccctaaggag ataaggcaaa
    24901 gaaccaaatc aatatacgaa ctcgatgagg ccgatgtggt aaaatcatcc cacaaaaacc
    24961 ccgttgtgaa aagtgtttac gatgattttt tgaaagaacc atgcggtgaa cttgcccaaa
    25021 agtttcttca tacttcctat tacaagcgcg acgtgctact atgacaagct agctaagttg
    25081 ggaggtctgg ccgatgctaa ttcgaaacgt gtaggtaaaa aagataatta atagtaaagt
    25141 ccatgcagga ggaaaatcgc aagctggtat atacaataaa ggaactttgt agggtttgct
    25201 atacctgtgt gagggagtgt ccggctaaag ctatcagaat aatcaatggt caggctgagg
    25261 tgattgccga acgctgtata ggatgcggga attgtgtgaa ggtgtgcagt caggatgcaa
    25321 aagtttacct gcaaacacga atggaggtca gggatttact gcagtcggag gataaggtag
    25381 ccgcccttgt agctccaagt tttccggctg agttcagcga aattgatgat taccggaagt
    25441 ttgtaggcat gctccggcag cttggatttt cctatgtttt cgaggtagcc tttggtgccg
    25501 atttggttgc tcgaaggtat aaggagttgc ttgaagaccg aagcggcaaa ggctacatct
    25561 cctcggattg cccggcaatt gtaacctaca tacgatacta tcatcccgat ttaaccgaat
    25621 acctggcccc ggtggtatca cccatgattg ctataagccg cgtagttcga aaaaagttgg
    25681 ggaatggggt taagttggta tttattggcc catgtattgc caaaaaagcg gaaaccgatg
    25741 aggttgatga ggcgctaact tttcgggagc tacgaggtat gctggaatac gcaggtgtaa
    25801 cccccgaagc agcaaccccc tcggaatttg acctaccccg tgccggacgg ggtgctatat
    25861 tcccggtaag ccgggggtta attcaaacgg tacgggtaaa cgaggatttg gtagaaggta
    25921 acgttacggt ggctgaaggt cgtatgggat ttcaggaggc tattaaggag tgggaaattg
    25981 gaaaccttaa ggggcagaac ctcgagctac tctgctgcga tggctgcata atgggtcccg
    26041 gtatgtcgcc tggtggtaaa cgatttgaac gtaaaaccca gattagcagg tatgcccggc
    26101 aaaagctttc ggagcttgag catgatgagt ggctttatag catgaacgaa tttagggata
    26161 ttgatgtttc cagatctttc cagcgtgatg ataggcgaat gcctatacca tcagacgatc
    26221 agataaacag ggtgctggcc agtatgggaa aattctcgcc ccgcgaccag ctaaattgcg
    26281 gagcttgtgg atatgaaacc tgcgttgagc atgccattgc cataattgat gggttagccg
    26341 aggtggaaat gtgcttaccc tacgccatcg aagaacttca catttctgtt gacaatcttg
    26401 ccatttcaaa cgaaaagttg gcaaaaatgc agcaagcgtt gaagcaatca gaaaaacttg
    26461 cccatatggg gcagctttcg gctggaattg cccatgagct aaacaaccct ttaggtgtag
    26521 tgctgatgta cagcaatatt cttatggacg aatgcaagcc cgacgatcct ctacgcaagg
    26581 atttggagct gatagccaca caggccgagc gatgtaaaaa aattgtgggt gggctgctca
    26641 actttgcccg taaaaatcag gttaaagtag aaagggttga tatacgaaaa cttgctgcag
    26701 aaagcgtaac ctctgtagtt atccctaaga ccatttcaac tcaggtagta tgcaaggcta
    26761 acaataccga ggcctatctc gattacgaac aaatgatgca ggttctaacc aatctaaata
    26821 agaatgctgt agatgccatg ccaaacggag gggtgcttac cgttgaggtg gatgagaatg
    26881 aaagcgatgt aatttttagg gtgagcgaca ccggaacggg gatacctgag gagaatatgg
    26941 ataaactttt tacccctttc ttcaccacca agggaactgg caaaggaacc ggtttggggc
    27001 tggccactac ctatggaatt gttaagatgc acagcgggaa aatagaggtg gactccaaca
    27061 ccgatcccgc caaaggcccc actggtacca cttttaccat tgtgcttccc cggaaaccta
    27121 ctttaggtta aatgttcaat ttttaaattg atggatcatg cctactcctg caaagaaaac
    27181 cattttaatt gttgacgatg acgttgacta cctgtaccag ctgaaatttc aggttgaaaa
    27241 aatggggttt gaaacggtaa ctgccgaaag ccagcacgaa gcggaaacgc tgcttaacac
    27301 cattaaaccc gacctggcca ttctcgacct aatgatggag agcgaggata gtggttttat
    27361 attaagctac aagctaaaaa ggaagtaccc cgatgtgccc attatcattg caactgctgt
    27421 ttcggccgaa actgggatga cattcggaat caacaccgag gaggaacgga agtggattaa
    27481 ggccgatctt tacctggaaa agggcattcg ccccgaccaa cttcacaggg agattgttaa
    27541 actgttaaaa ctttagcacg atatggaggt tgttgacgtt ttggtggttg acgatgagcc
    27601 aggaattcgc tcaggtgtct cacgaatact taaaaactac accattaact atccattttt
    27661 ggaggacgac attagcttta atgtggttga agccgccaca ggtgaggagg ctattgagat
    27721 tatccagcgc aagccgcctg caatagttct tcttgacaat aaacttccgg gaattcaagg
    27781 aattgatgtg ctccagttca tcagtgctca tgaacccaac atcctggtta tgatgattac
    27841 ttcctatgct tcgttggagc tggcggttaa agccaccaac attggcgctc acgattttgt
    27901 ccccaaaccc tttactccgc aggaacttcg ctcgtcaatc gagaatatta ccaagcacta
    27961 cttcctccgt caaatgaccc gaaaactcca tcgcgaaggc aaacaggtga ggtttgagtt
    28021 tttaacgatg ctgtcgcatg agctcaaagc cccactgaat gccattgagg gttaccttag
    28081 aatgatgcag gagaaacagc ttggcgaaag tttggatgac taccaggaaa ttatcgaccg
    28141 ctcgctttcc cgtattcagt ccatgcgcaa cctgattatg gacctgcttg accttacacg
    28201 ggtagaatcg ggtaagcggg aaagagactt gcaggatatt gaccttaccc tggtagctcg
    28261 gctggtggtt gaaaccatga tgcctatggc catacagcgc gatgttactc ttaccctcga
    28321 atctgatgag ccggtagttg ttaaagccga tgccgacgac atggaaattc ttcttaacaa
    28381 ccttatatcc aacgcaataa agtataacgt agagggggga tgggtgaaat gtaaattcag
    28441 caaaaccccc acctatgcaa cctgcagtgt ttctgactcg ggtgttggta ttagcgctga
    28501 tgacattcca aagctattcc acgagtttac ccgcatcaaa aaccccctta gccggaacgt
    28561 tagcggaagt gggctagggc tttccattgt aaaaaaaatt gccgatcagt acggcgccga
    28621 gataaaggtg gaaagcacgt tgggaaaagg ctcaaccttt accctccaat ttccggtatc
    28681 tgccgaaaac cctgcctagc tagcactgac ctttgttaaa catatatctt tcttttactt
    28741 cataacctaa agaaaagcgt aacattttta aaaagatatt tagttttgtg catgattcta
    28801 cattttcgct attttcaata cgagttgaag ttggcgaaga aaatagttta ctagtccaaa
    28861 gctatataaa tttaccttta aaacatgcat agccttatgt tcataggcag gctaaggatt
    28921 gtgatgaata taaagctgaa cttgctaagt caatttaaat tttattaata gaatgaagct
    28981 tcccgagaaa accgttgaaa ggctgagtga atatcgtcgt accctgctaa gttgtatagc
    29041 taaagggaaa acacacattt actcccacga gttggccgga attcataaca tcacagctgt
    29101 tcaggtaagg cgcgatttaa tgcttatcgg ctactccagc ataaagaaaa aggggtacga
    29161 cacccaggag ttggccgatg taataggcgc ccttattgac cacgagaaag gcttaaatgt
    29221 tgccgtagta ggaatgggca acatggggcg tgctatcact tcatacttca acggtaagcg
    29281 gccaaagctg aatattgttg ctgcattcga cgttgatccg aataaggtta accgtgttat
    29341 agctggtgtt aactgctttc acatgaagga tttacttgaa atttccaaac ggtatgagat
    29401 ttcaattgct atcattactt ccccgcccga ctcggcaacc caggtggctg aacagctggt
    29461 ttctgccggg attaaaggaa tccttaactt taccaccatt ccattaaatg tccccgatac
    29521 agtttatctt gaagaatttg atatgattac ttcattggaa aaggttgcct atttcgttaa
    29581 aactaaggat gagtagccgg taaaacttca accttaaacg ttgtctccta aatgcaggta
    29641 cactatggtt ttgataatgt tggctatatt aagaatcctg tggttacaac gggctctttt
    29701 gatggcgtgc atataggcca taaagccatt cttaagagaa taaacgatat tgccagaggt
    29761 attgacggtg aatcggtgct tattactttt tatccgcacc cccgcaaggt tctgtacccc
    29821 gaaaatgctg gtaagaatct ggtcttcata ctttcacaaa aggaaaaaat tgaactttta
    29881 agcaaaacag gtcttgatca cctgattatc gtcaggttta ccctggagtt ttcccatatc
    29941 tcgtcggttg actttataca gcatttttta atagcaaagc ttcacgcccg gttcgtagtt
    30001 gtcgggttta accaccattt tggccataac cgcgagggag attttgaaga gctgaaacgc
    30061 cttggcctgc agtacaactt tggagtagag gaaattccag agcaggatat tcagcaggaa
    30121 accgttagct ccactgcaat ccgtaaggct ttgctcgagg gtaaaattca acgggccaac
    30181 gcctacctcg accatcactt tattattatg ggtactttgg gcaaaggtgc tcatattttc
    30241 cagagagcag gattccccac ccttaccgtt caaattgagg aagcgggaaa gctgataccc
    30301 cacgagggta tttacgctgt caccctcgaa tggaatagca cccattaccg cgccatggcc
    30361 attatttggc tcgacaatta cggcagtaat gaacccgttt tagggccact caacgttgac
    30421 gttcatataa tggagtttga taatatcctt tccgaggatg atgcaaagtt gtttttccaa
    30481 aaacaacttg ccgatcatgt tgacatagct gatgaggaac gcctgactag tcagctttcg
    30541 gcagcttgca gctccttgga tgagctcatt ttttaaggag cagctccgat aaattttaaa
    30601 gctttttgtg ctgaagcata gcgtaactat ttgtaacttt acgacgtaat taaacttgta
    30661 tttcaaggtt aaaaatctgt atccgggaaa ggaattttaa ttgctttcga ggattcagct
    30721 aacagttcta atccaaagga gaactctgat ggaaaggaga atcggggcgg cgcttatcct
    30781 ggtggagaac cggggaagcg tggagaagtt aaaccagcta ttttcggccc attccagcgt
    30841 gattgtcgcc cggcagggga ttccgcttca gcagcggggt gttagtgtta tctcgttggt
    30901 gctcgatggc accagtgatg aaattagcgc actaaccggt ccggtaggca agctcgatgg
    30961 cgtacaggta aaaaccctca tgatgaaagg gtaattctac ctattttctc tcctttacat
    31021 tagaattgcg ctattctaac ctatcattag gttggagatt atgggtatgc aaagcatatt
    31081 cattcccatg aagtatatat gttatgggga agatttagta cgaagcgtga aaaaaatgat
    31141 gaattactta aaattatgaa atgaggggca ttgcaactct ttgcatgcat taccaactaa
    31201 atattaacaa ttaatttgca aacaaatgaa atttaatcct gaagtttaca gcattcccga
    31261 tgagcgaatg aaacctttca tcgatcccga tgaaatttgg ggttacatca acagcgccaa
    31321 gcccacccgc gaaagggtga gggaaatcat tgccaaatcg ctcgacaagc atcggctaaa
    31381 ccttgaggag gttgcagttc tggtaaatac ctccgagcct gagcttgttg aggaaatcct
    31441 gcagggagct cgtacactta aacaaaaggt ttacggcaat agaattgtcc tatttgcgcc
    31501 gctttacatc gggaataagt gcacaaacaa ctgtaagtat tgcgggttca gaacctccaa
    31561 caccgaagct gtgagggtaa ccctttcgga tgaggaaatt accaaggagg ttgaggcgct
    31621 tgaggataac ggtcagaaaa ggctgatact ggtttacgga gaacacccgg agtactccgc
    31681 cgactacatt gcccacaccg ttcgcctggt ttacggggtt aaaaaggata gaggtgaaat
    31741 tcggcgggta aacattaatg ctgcaccgct aagcgttgaa gattttttga aagtgaaaaa
    31801 ggcagggatt ggcacctacc agatttttca ggaaacatac aaccctgaag cttatagttg
    31861 gtaccaccag gcaggtaaaa agcgggattt tgaatggcgg ctcacctcgc tcgatagggc
    31921 acaggaggct ggtttagacg atgttggaat tggtgcattg atgggcctgt atgactggcg
    31981 ttttgaggtg ttggggctgg ttaggcatac caaccacctt gaggcttgtt acaacgttgg
    32041 cccccacacc atatcttttc cgcgtataaa gtcggcttca ggtctggata ttaccaacca
    32101 gtattttgtt tctgacgatg actttgtcaa gttggtggca atcctgcggc tagctgtccc
    32161 ctacaccggc atgattctta ccgctcgtga acccgtgcat gttaggcaaa aggttttgca
    32221 gtttggcgtc tctcaaattg atggaggaac taagctggag ctgggcgctt atattgagtc
    32281 taaaaacgag aagcaaaatc ttaatcgtga gcagtttgaa attggcgaca gccgatcttt
    32341 gggcgaggtg gttgacgatt tggtggaaca tgattatatc ccctcgtttt gcacggcatg
    32401 ttaccgcctg gggcgaaccg gcgagcattt tatggagttt tctgtaccag ggtttatcaa
    32461 acgttactgt accccaaatg cagttctcac cctaaccgag tatctggttg actatgctac
    32521 acccgaggtt aaggataagg ggtacaggtt gattgataaa accttgaccg agctggatgg
    32581 aaatcagaat gtagaggaaa taaaacgtcg tatggagcga ataaaggcag gtgagaggga
    32641 tttatacttt tagcaaaggt tccttccgtt tttactagaa ttatgtgatg cacagcttat
    32701 aaccctgtat agcccatcta ccttaatagc agatgggttt ttttatgtaa agtgttaacc
    32761 atagctgtca gctaatccca ttcattttgc gcgtagcaag tgcggtttta ggaacctgcc
    32821 atctgtccac gacgcgaatc ggagtataac atcaacccat ctaaaaataa gcaagatatg
    32881 tagaactctg gaaaaattgt agagaattaa tctgaaaggc ttaaaaatgg ttgaattggc
    32941 tatttaaact ttattagaac tcaccctttt tttttgccag atttttcacc ttgccaattt
    33001 ccataaaggt tgcgccattg ctcgggccaa agctgccccc aataataagc accatgtcct
    33061 ccgaggcaat tttacgctgt tcaagcatgt agcttattgc gtcgctgagg aaatggtcgc
    33121 gtgattgacg cggttccatg tagaatgcct caattccgta tgaaagtgca agttggcgca
    33181 taacatgctc gttgtagcaa attgcataaa taggaatttg gcctctgaat gccgataggt
    33241 aacgtccggt gcgcccggta agcgtgtcca caataattgc cctaacgggc agcattgaac
    33301 atgccctaac cgctgatttt gcaagagctg cagtaacttc attgttaatt ctagccagat
    33361 taattgacga gtcggtctcc aaaacatctt caacttcatt tgccactcgg gtcatcacgt
    33421 taacagcctc aacaggatag gccccataag ccgtttcacc gctaagcata atggcatcag
    33481 cccgttggta tatggcgttg gctatatcgc taatttcggc acgggtggga cggggatgct
    33541 caatcatggt gtgtagcatt tgggtggcga tgataaccgg ctttttgctt tcaatgcatt
    33601 ttcgtacaat ccggttctgg ataatgggta gtttttcgga aggtatctca atgcctaaat
    33661 cgccccgcgc taccattatg ccgtaggcat ggtcgagtat ttcgtcaata ttatcaactc
    33721 cttgctgatt ttcaatcttg gctataactt ttattgggct tttttgctgt tgaagtattt
    33781 cctttatttc aagcacatcg tgcttattcc tcacaaagct gtgggcaata aaatcaaccc
    33841 tattttcaac ggcaaactgc acaaaatcct tatccttttg ggtaagcgag ggaaggttga
    33901 tatgcacacc aggaaggttt acgcttttcc gtagctttat tacacctcca ttttcaactt
    33961 cgcataccag ggcatcgtgg ctcacactcc ttaccctaag ttcaatttct ccatcatcaa
    34021 tcaacactgc gtttccaaca ggtacctcct cgtgtatgtt tgggtagcta acaaaaatgt
    34081 tgtcgccacc cgattctccg ttcgggtcgc ccgatatctt taccgtcata ccttgctgaa
    34141 ccttaacctc agctccctta gatgaagtcc ggatttctgg acccttggtg tcaaccagaa
    34201 tggcaatatg gtcggatact tttcggatgt tatctatcaa tattttagcc ccgctgggat
    34261 tcatgtgggc ggtgttaagc ctggcaacgt tcattccggc atcatacaac gacttgatga
    34321 aggagacctc acaccgctgg tcggatatgg tggccacgat ttttgtcctt ttaagcatac
    34381 tcgctaaatt tttcggtgca aaactactca atatttagag aaacataaat tttttaaata
    34441 gcgcccagaa ccatatacga gggcgataac gtataaattt aaaagaaatt cagaaaactt
    34501 tgtaaaattg ccttttcgtt atgtttattt gcagcaaact ctgagtatgg cgatttgtat
    34561 tttcgttata cttataaatc tggttgtatt gtggtgaaag aattgtaaaa gaatggtttt
    34621 atgggatgga gcgccgctat tgaggattat attaattttt taaagctgga aaaatctctc
    34681 tctaaaaatt cggtggcagc ttaccataca gacatgatga agttgtatgg ttttgcagag
    34741 attatagggg tagagcccga atcggttacc ggagagcaaa ttcaggactt tattgcacac
    34801 cttcacgata gcgggttgag taaacgctct caggccagaa tagtttcaag tgtaaggggc
    34861 ttctacaaat acttgcttat tgaggaggtg gttgacagcg atccaactga gcttatagag
    34921 ctgcctaaaa ccgggcgaaa gctgcctgaa gttttaagcg ttgaggagat tgatgccatg
    34981 gaggcagccg ttgatttgag tatgcccgat gggcatcgca acaaggcaat catcgaaacc
    35041 ctttacagct gtgggttacg tgtttcagaa ctggtaaacc taaggcttac cgaccttttt
    35101 tttgatgatg gttacgtgcg ggtattggga aagggaaaca aggagaggct ggtacccatt
    35161 ggccgtaagg ctatagcgga cattaacatg tacttgtcgc agcgcataat ggtaagcatt
    35221 actgttgatg ccaagtctgg gaacatcgtt tttctaaatc gtaggggaaa acagctaacc
    35281 cgcgaaatgg tttttctcat cattaaaaaa tatgccaagg cagcagggat aaccaggaat
    35341 attagcccgc atactttaag acactccttt gccacccact tggtggaggg aggtgccgat
    35401 ttgcgtgcag ttcaggaaat gctggggcat gaatccattc tgactaccga aatctatacc
    35461 cacctagaca aggaatttct ccgtgaaacc attttgaaat ttcacccaag aggttaactt
    35521 tgtgcaaaca tccttaaatc atttagctat ggatactgga gaaaaaattg ttaaaaccct
    35581 atcatctgct ggaaaaccga tgaaggccgg agaaattgcc gaggctgccg gaattgataa
    35641 gaaggaggtt gacaaggtga ttaagaagct ggttgccgat ggcaagctcc attcgcccca
    35701 acggtgcttt tatgatatta aaaagtagct ttaaaccggg ttagccgcta acccggtttt
    35761 tttaatcttt cgggtgattt ttgacaccct taacatacaa gggtttacat gaatttcggg
    35821 gaagaaaagt taagcgaaaa ctttacaata aatgtcttgc aggcatggtt ttctccttta
    35881 aagaatttca aacctgtcgg catcaagatg agccggaaaa cttgcacgaa tttcatggag
    35941 ttgctccaat gagattgaag cttcaatagt tgattcgctg ttgtggtttg ccttagctac
    36001 aatttgtccc attgggtcaa ctacctgaga atcaccagaa taattcgccc cgctgccatc
    36061 aatgcccact cgattgcagg cggctacaaa gcactgattt tcgatagccc gtgcaaccag
    36121 tagggaattc cacgcatagc ttcgttcatc cggaaagtta gctacgttga gcatcaggtc
    36181 gtagtcattc ctgtttcgac accaaacagg gaaacgcaaa tcgtagcaaa tggtaagaag
    36241 tatccgccag cccatgtagc ttactactac cctttggtta ccggcggagt attggttgtg
    36301 ctcaccggcc atcctgaaga ggtggcgttt atcatagaaa acaacctgtc cgttgggcat
    36361 aacaaaagcc attctgttca cgagtttgtt ttctttccgg gcaataatgc ttccaacaat
    36421 agcggctttc cttaccatgg aggtttgcaa catccaatca agagttcgct cggcttcact
    36481 tgcatcgcat tccctgggct tcatggtgaa accggtggta aacatttcgg gaagcagaat
    36541 caagttggta tctgttgaag tgctttcaat tttggcggta aaaagctgaa ggttttcctc
    36601 tatgctctcc caaaacggcg atgcttgaat gatggatatt ttcatcgtgc agcttaggtg
    36661 ttggctattc ggtataatgc tgcgaatata cctaaatttt tttctgggtt aagttttagg
    36721 cttggtatag gcacttggct gttgtgaaaa agcgcttggt caactgctca atgcttaatt
    36781 gaacactccc cggagatttc tacctttctt tgcagggctt atttgctgct tcctgtgaaa
    36841 ctatgccatt cggcttttga tttctcaacc agcctttcat actccattga tatagcgtta
    36901 attacaggta tggaggcgtt catgctcacg ttaccaacct gtctaacggg tagaactcct
    36961 ggtgaagttc ctgtaagaaa gcaaccatcc ataaacgcca gctccttaat gcctatacat
    37021 ctttctacta gttcaacgtt caagctatgg catatttgca caactttttg tctggtaatt
    37081 ccacttagca ctttagtttc tggcgcagtg aataatctat cacggctgat aaagaaaaca
    37141 ttggaacggc ttccttcggt aatttccccc tgggcgttta ccaaaagaac ctcgtagcat
    37201 ccatgacttt ctatgatctc gttggtaagc tcgcgcagcg atttgttctc aactttaacc
    37261 gtcgggttag gcctttcggc atcaatacat tccagaacaa cgcctttctg tttttcaatt
    37321 ctggtgggat accttgaagg aatgtagtaa accgttaaat tccggaccgg atgattggtg
    37381 gatacgctgt aggttatacg gacattcttt tcagctacag ggtttttctc agtgagcatt
    37441 ctaagggaac tggcagctga attatttgcc aatgcaggca ggttggaatt tttgattgag
    37501 gcttgcaggc gcaataaatg ttcaattgca aaaatgggtg ttgagccagc aacccttact
    37561 acttcgtaaa ccaccgtttc gccctcaacc atgctatccg taaaattggt agttgggagg
    37621 agttggccgt tatgaacaaa aaatcgctcg gttaaatcag ctggaaaatt caaggcttta
    37681 atttgctagt agtactataa ctatcaacca tttagctcag gatgatctcg gatttctggg
    37741 aatgtccagg aaagttgatt cttgctttat actggtaacc ttgcaaaaat agcaggaaaa
    37801 actaatattc agcatggata tttctagttt gtttcagcgg agttgggtaa aatccgaagg
    37861 ggtgggtaaa ttaattcctc atagtctcga aagttgggaa aagtgttaat ttccctgttt
    37921 tctcagccta taaatttggt aacggggaat tgatagcaga atctatgtgc attggtttgt
    37981 tcctcctgtt cgaattaaaa gttgacctgt gctgaggtat ggactagcta ctgcgtctta
    38041 tttatatagc tgttcagaat gttttccatt agcggggagt aaaacttgta gaagaattga
    38101 gaattttccg atttaccttc aagcagctgg ttcaggaatt tgccccccat cattttagag
    38161 gtgaacatgg taactttgtt ttcggtaaag tctccggcaa agtagaaggt gaactcattt
    38221 gatttagctt gtattactgc ggggaattca ttagtcaatc caattgccct gagtttttgt
    38281 aagccctgtt gggtagtgtt aatcttaaaa gttgaagggg taacagcgtt actaccggga
    38341 acaacaaatt caaaccaacc cgcgaatgga atttccgaaa tcaaaccaaa gcgagatatt
    38401 cctgtattgt ctgaaataat taccgggaag gcagaattta aatcttcacc atcaactagc
    38461 acatcaacta gcccatcatt gctgagtaag ataatgccct taccttctga tggccatggt
    38521 ttaaggtgtt gtgattcata caacttgggc atccatggtg cagggccacc agcctcgtta
    38581 ggggttaagt tgcggcagaa tactcccgac caacccgtcc agctgacatt gaatagctct
    38641 tcggctttac ttctcattag tgcgttggtt ggagtgctga acatattata ttctcctatg
    38701 atgagttttc ccatatcctt catgctcttt agaagaaggt agtcgttctg gttaaggcca
    38761 ccataaactt tagaggaacg tataggttgg gcatcgtttt tataccattc aaaagagtaa
    38821 acaccataac aatcggcgta gtatgctgca tcgtatgcgg cagcataggc atcaacctca
    38881 tttagccgta tagccttaaa gtcgaatagc tgttggttca aatcaatggg gaaaaatccc
    38941 cagtaatcct cattaacccg ataggtcttg ccatcgggac caacaatcct gtcctggtta
    39001 agcacccaaa ccaaggccct atgctctggc cgattgctcc gggtaactgt tttgtccagg
    39061 atatagatat ttagtttttt tgagggctga agctgccaga atatgaacag caagattgcc
    39121 actagcccaa taacgctaat agcaaccatt actcctttat aaatcttttt catctatcta
    39181 attatcttat ggttagctgg ttgagggtga aaatgtttgt caattctgat gtgctagagc
    39241 ggcctactta gcgctgttcg ttctgactgt cggttatttg tcttacatcc aaacgccata
    39301 aatggttaac tttaggtagc ttatgcctgt atattgatta tttttaaaag ttaaactcca
    39361 aaacgtttaa atccgcatgg tttaagtacg atacggccaa tttacgaaaa attaattatt
    39421 cggtggccaa attgttgatg gctaagacga ttttttactt atacttttaa ttttacagcc
    39481 cgaagagcca atacgcctgc atgtgattag cctttataca ggttttgcca cagctaaccc
    39541 tcgggtaagt gcaaaaagtt ggatgagtgg ttggtgcgga aatattattt cttagaagtt
    39601 cgatgagtta ttatgcaagc caatgccatt aacagcttga gccacatcag cggccgagtc
    39661 catagggagc ctgcaggagc tacctacaca tagctgaaat ttatggtcaa cctttttagc
    39721 gatcaactca aaataaatgt tagggtatat taccctgcct aaaatgtcgt taacatcgtt
    39781 tgatgtgccc tcgaatatgg ttatgctaac ttcaggaagt gcaagcgaaa gcataaggtt
    39841 tccccagcca tacaagaata ctccgttgtt tttagtattt tcagccaaat tggccagcac
    39901 ctgcatggct attttgctgt aaagggggtt ttcaatatgc ttagccagaa aaagcaaatt
    39961 tccggtcatt atggaggatg aagatggaat aaccccgtca atcagctcca tttttcgaac
    40021 catcagatca ctgcttcccg gggtgtaata gaacatacct ttaccctcat cccaaaaatc
    40081 ccgtatggcc tcctgcgcta atgaatttgc tttaaacaac catttttctt ccagcgttgc
    40141 ctggtaaagc ataatcaggg cgcgtatgta gttggcataa tcgtcgagca gggcgttacc
    40201 ataggtttta cccttgcaga aaacccgttt caggttgcct tcaacaaaat gtttggtttc
    40261 aagatagctg gcaatttcgc tggcaagccc aatgaaacgt ggatttttaa aggtgatgaa
    40321 agcctgcgcc agggctgaaa ctaataaacc attccatgat gctagaactt tatcatctat
    40381 tagcggagga atacgcttgt ttcttatgct ggcaagggtt tttaggctgc gctgcagcct
    40441 gaactgaatc tcctgcggag ggatatcgaa taagctgctt agctgatcat ccgaagcgca
    40501 acgtttaagc ccatttctgc cattaatatc tcccgaaggc gttaccccat atgccgagca
    40561 aaaaatttcg ctatcaaatt ccaggacttc gtcaagttcc tgtttggtcc atgtgtagaa
    40621 gcctccctcc agtccattcg catctgcatc aagcgagcag taaaaaccac catcgggtga
    40681 atgtagctcc ctgtcgagaa aatcaaccgt gttaaacact atgttcaggt atgatgggtt
    40741 tgggtttatc cggtaggcca gggagtagag ctcaacaagc tgagcgttgt cgtagagcat
    40801 tttctcaaag tggggtgtgt gccacctgga gtcaacggca tacctaaaaa aacctcctcc
    40861 aacatggtcg taaactccgc cattggcaat attatctagc gtagtggaaa cgaagctttt
    40921 aagttttaga ttaccggtag cctgtgcgct ctgcatcaga tagcctagca gccccggcat
    40981 cgggaatttc ggggcaccac gcgttccacc caggccgtaa tctaaagcag gttcaatgga
    41041 ttgcaggttc cccattacta ttccggtaaa atccagctcc gtcagtttcg tcttgacatg
    41101 gattatctcg gtgttggcaa ttccttctgc caagtcttcc gctacctcgt aaaccctttc
    41161 gggttcgctc ttccaggtgg catccatgct ttcaagaata ttcaaccatt gatcttttgg
    41221 gaagtaggtt ccgccgtaaa caggccgccc gtcgggaagg gctatgcagt ttaatggcca
    41281 tccaccccgg cgggttatta tctgcacggc gctcatgtag atgtggtcga tatcgggacg
    41341 ctcctcccgg tcaaccttaa tggaaacata cttctcgttc atcaacctgg ctacctgctc
    41401 gtcggtaaat gattcttttt ccatgatatg gcaccagtgg cagctgctat accctatgct
    41461 gatcaccacc agcttatttt ccctttttgc aacctcaaaa gcttcgtttc cccatggata
    41521 ccagctgact gggtttaacg agtgctgtaa caggtagggg cttacctcat ttgcaagttt
    41581 attgggctta tcaaacatta acttttagga atgtgggttt agtaactttt ggtattgcat
    41641 ttgttccatt tatttcaaaa caactacccg tgcacaatgt tgatgtatgt tccgagaatt
    41701 attcattttg caagtttatt tttctttttt ctatttttga ggtgaaaata ttggtcgtta
    41761 ataaattcat gtaaccatga aaaatttgct atttgcccta tcgttttttt tcctttcgtt
    41821 tggtgtattt gcccaaaacc acaacgggta tagaattgag attaaaattg atgggctgaa
    41881 ggataccacc ctgcttttgg ggtaccactt tggtgataag aaatttgtga gcgatacagc
    41941 acgtatagat tctaacggtg tagccatttt tgagggtgat agcttgctaa aaggtggaat
    42001 ttacctggta atcctaccgg acaaaaccta ctttgaagtt cttttaagca acaaccagcg
    42061 ttttggagtt caaacaacgg ctaaaaacat ggttgaaaat ctgaagttta caggctcaaa
    42121 ggagaatgaa gaatttattc attttcaaca atttatgatg ggtgttcagg ctcaaagcaa
    42181 tgagctatcg gccaaaatga aggatgccct taccgattca accaggtcaa accagcttcg
    42241 gaaagaaatg aatgagctgg acgagaaggt gaaggcctac tggctggagg tcgagaacaa
    42301 caacaggggc acatttctgg catcaattat caaagccatg agaagtgttg aggttcctga
    42361 atttacgatt cctgctaaca cgcctgctta cgattcctta aagtgggttc acagctataa
    42421 ttttaaccgc gatcactact tcgacaatat tgatttcagc gattcacggc ttcttcgcac
    42481 cccaattctg gagaacaaga taaacattta cttcgatagg gtgcttatac cccatcccga
    42541 tagcattgca gcggcagcag ttaacctggt tgaaaaatcg aaggcaaaca gggagatgtt
    42601 ccagtacatt ctttcatacc taattaacaa gtttcaaacc tcagaaagga tgggcatgga
    42661 tggcgtgttt gttgtattag ccgaaaagta ctatcttgga ggccaagctt ggtgggccga
    42721 ccagaaactt ctcgataaaa tttccgaaag ggtaaatgca ctaaagccca accttattgg
    42781 taaagttgcc cccgacttat ggctacccga tccgaatggc aagtacttta ggcttagcaa
    42841 tattaattct aaaattactg taattttctt ttgggatcct gagtgcagcc attgtaaaaa
    42901 ggagatgccg aagctcaaaa acgtttacga caactacaaa aagtatggcc ttgaggtgta
    42961 tgccgtttac acccagggcg accagcccaa atggatggaa agcatttcta agaacgactt
    43021 aacctggatt aatgtttggg accctacatt cagctctaat ttccgaaacc tctacgacat
    43081 ttactccacg cctgttattt acgtcctcga ttccggtaaa aaaattatag ccaaacggat
    43141 tagcgttgaa accctcgaat cgattctaaa gcaggaactc aagctgtagt gattttttcc
    43201 tcagtaaaat ttcagcttaa gggttgaaaa atcctgaaca ttcccgtaaa tgattttgac
    43261 caagattagc agacccttca ctgttgtgtc tggttattgg tcatggttgt cacggtatat
    43321 ttgttgataa atccgaacag aacgggttgt tttactttca tattgttagc caatatccga
    43381 aatctataaa aattataagc tgttttaggc tagaggatgg aatttttact atcttggccg
    43441 caaaactcta atcaacttaa catttaagat ttatggagag cttactgacc aatacctact
    43501 tttgggtagt gttagcctat tttgccgtgg ttttgtttat ctcgtttttt acaaaaaagc
    43561 ttgctagccg atcgtcagcc gacttcttag ttgccggtcg aaacctgggg cttctggtgt
    43621 gttcaatggt ggtagccgcc gaatggctgg gtgggttgag cacagttggc gttagcgaaa
    43681 aggcattcga cacccttacc ctgcaaccta ttctctataa tttttccaca gctattggaa
    43741 tggtgatact cggctttact ttagccaaag cttaccgcga acggaaggtg aatacggtga
    43801 gcgaaatggt agagagcatt tttggaaaac gtgcccgaag catatcggct attgcctttc
    43861 taattgcttt tataacgctt gcctacgtgg agctaaccac ttttgcaact gttctttcgc
    43921 caatttttaa cataaattgg gtttgggccg tcataattgc agctactata accacaattt
    43981 acacttacat gggcggaatg cacgccctca ctattgttgc tgtgttcaac cttgttgttc
    44041 ggtatgttgg gttgggaatt gccctaatta ttgggcttgt tgcaattggc gatttttcaa
    44101 atttaaccaa caggcttgtt gagattggcg ctcctcaaaa tttgtacaat ccttttagca
    44161 taggatggaa tgctgcgttg agccttatac tgggtggaat ttttggtgga atggcttccc
    44221 aagccagcat tcaacctatt tttgcagcta aaaatcctat taccgcaaaa agggctgcca
    44281 ttttatcggc agtttttata gctccttttg gaattatcac aggaattctt ggtcttattg
    44341 cccgaagtgg tttgtttttt gaagttaccc ccgggtttaa ccccaaaatg gcccttccac
    44401 gcttgctaat gacccccgaa tttgttcatc ccgtgattgg gggaattgca attgcggcaa
    44461 ttcttgcggc aattttatct accatcgggc cggtgagctt ttcaattgtt accattgcta
    44521 ctaaggatat ataccatggg ttgataaaca aaaaggcatc cgatgagaaa atcctccagt
    44581 ttgctaaaag gctcgtaata ctggtaaatc tggtaaccgt tcctttagcc ctgtttatga
    44641 agggcgcaat tcttgatgca gggtattttt cctatgccat tcgaacaatt ggtgcaatag
    44701 tgatactttt gggtatttac aaattgaagt ggatttctac cctgtctgtt cagctttcct
    44761 ttattggcgg cacgttggcc atcattatta gcattgtagc caaaaagttt ggttggtttg
    44821 acattgataa aacctatgcc agcatcattg caacagtatt tttcattatt ataggcaaca
    44881 tctacacgaa gtactttaca ggtaaacaaa aatcatcgga gttaaatatc aatgaatcag
    44941 ctactaaatg acataagggt tcttgagctg gccaacgtgc tggccgggcc aagtgttgga
    45001 atggcgttgg ctgagctggg cgccagagtg gtaaaggttg agaacttact taccttgggt
    45061 gatgttaccc gaacctggaa attaccatcc gaagatcccg atacaaatat ttcgggatac
    45121 ttcagctgtg ttaactgggg gaaaaagtcg ttggccatcg atctcaacaa acccgagggg
    45181 cttgacattg tttacaggct aaccgaggtt tcggacatcg tgctggttag ctataaaccc
    45241 ggcgatgccg aaaaactgaa ggtggactac aataccctta aacaatacaa caaatcgctg
    45301 atatacgccc atctaaccgg gtatggatta aagaatgaac gcgctggata cgacgcaatc
    45361 attcaggccg aaagtggctt tacctacatg aatggtgaac aaggagggaa acctgtgaaa
    45421 atgcccgttg cactgatgga tatacttgcc ggacatcagc taaaagaggc aattttgctg
    45481 gctctgctga atcgctttag aaaaggtgaa gggacttata ttgaggcgag ccttattaaa
    45541 tcaggagttg ccgctttggc aaaccaagcc accaactggt tggtgggaaa ttctatcccc
    45601 cagcgcatgg gttccgatca tcccaacatt gtgccctatg gaagaatttt ttatacccgc
    45661 gacgagaaac ctgtagtgct ggctgttggc agcgataaac agtttgccga gctgtgctca
    45721 atcttgggga agcccgaatt ggctcaggat cctaagtact ctaccaactt caaccgcgtc
    45781 aagaatcgca acgagattta cccaatttta caggatttag ttgcccaaat agaccgtgat
    45841 cctttcttaa atgaactcgc cagaaggcgg atacctgcag gtggagttta caacatgcag
    45901 gaggtgttcg accaacccga agcgcaggag ctggttatga gcggaacaac ttcagccgga
    45961 accccgataa agggactaag aacggtggct ttttcttaca atgagaattc caagccccta
    46021 gagttggctg ctcctccata ttacggtgag catacaattc aaattctaaa ggaaacgctt
    46081 ggtttctcaa atgattgtat tgaaggttta atcaataaaa atgttgtgta tgcacccagg
    46141 taaaaccagc ttggctttta tagatggatc gaggcttatt accgaatcgc ttgttcgtgc
    46201 cggcgccgat accttcatag gttacccaat tactcctgca aacttgctat acctctacgc
    46261 cacaaagaga tacccaacaa tgcttgctgc tcccgatgaa attacgacac ttcaatggat
    46321 gagcggcttt gccattgctg gacatgtacc agttacagcc acttcattcc caggttatgc
    46381 cctgatgctg gaatctataa acatggcggc catgtttgag ctgcccatgg taattgttct
    46441 tgttcaacgc ttaggaccgg caacaggtac ggctacttgt ggggcgcagg gcgatctttc
    46501 actggttcac ggtacccttt ccggcggtta caccctgcca acattctcgc caagcaactt
    46561 aaacgattgc tgggatattt cagctaaagc ccttcatact gccatcaggc tgagaactcc
    46621 tgttgtgcta ctcacatcaa aggaaatggt gatgaccctt caggctttcg actggtcaca
    46681 tttggcggag attgaaacac ttaaggttaa aacgtttagt ggcagcgata cctacattcc
    46741 ctacaaggtt gatgaatcgc ttgttcccga ttttatgcca gttagccaaa acaaccatgc
    46801 agtgcggtta accgcttcaa cacacgacac aaagggattc cttcaaaatt caactcccga
    46861 ggctatagaa aacacgaagc ggttggaggc taaggttacc aagcaccttc aggaatacct
    46921 tttatttgac cttgatgagc aagcaggagc tcaacatctg gttgtctcgt atggaatttc
    46981 tgcacaagct tcacgcgaag cagttaggca gctaagagca aggggcgaaa aggtgtcgct
    47041 gctggttgcc aagacccttc tgcccgtttc acgtgattat gttgacattg ccaaccgtta
    47101 cgaacgcgta gtggtggttg aggaaaacct taccggacag ctgcgccagc tgctttttgg
    47161 cgctgctggt cgtaatgggt taagcggtgt taatgctatt gcccgtatga taagcccttc
    47221 cgaaattgtt gacgaggtaa tgagaaagtt ttagaaatta tttacttatg caaaccctaa
    47281 tatttgaatt tcggaaatac tagaatgaat cggttatatt taaatcataa cgatttttag
    47341 aatgaacaac gtaaaatttt taaccggagg acaactaccc ttttgtaagg gctgcggaca
    47401 tgttgctgtt gcccagaaca ccgaaaaggc cttacaaaag ctagatttta acccccttga
    47461 cgtagttctg gttaccgata ttggttgcca tggcattgtt gataagaatt ttctcaccca
    47521 caacgtgcac ggccttcatg gacgctcttc ggccttagca gcaggaatat cggcaggatt
    47581 aagtaacccg aataagaagg tcatagtttt tacgggcgat ggaggtgcaa ctatcgggat
    47641 gcagcattta gtcggcggag ctcatcttgg attcgacatg acggtggttg tgcataacaa
    47701 catgctttac ggcatgaccg gtggacaacc ctctgagttt acaccctgtg gcttcaaaac
    47761 tccaaccctt cctgaaggca gcacaaagtc cggctacgat atttgcgaat taatgcttgc
    47821 tgctggcgca gcttatgttg agagggtggt tggaataggc gatttttctg attctcttgc
    47881 acgcgctttc tctaccaaag gcttctcgct ggttgaggtg atggaaattt gcaccagcta
    47941 cggggtaaaa tcaaaccccg gcatgaagct tcccaagctg gttgaggaag ccggatggaa
    48001 ggtgaaagtt tttacggagg ctaaacagcg cctctttcaa accccacaaa atagtaatcc
    48061 taccagcctg ctttccgaaa aacttgaggt agagccaaaa tactcagggg caataagtaa
    48121 gcctgtaagc attatgctgt caggatcagc aggcgaaggg gttcaactag cagctgaatt
    48181 tctggccaga gcagccatgc tgtcggggtt gtatgctact aaaaaaggaa gttacccggt
    48241 tactgtcgga gttgggtatt cagctgcaga ggtgataatt tcacacgaac ccatacttta
    48301 tactggctct cctgttcccg atatcctggc tattacttct gccgatggac ttgggtatgc
    48361 cagagctgca gcaggaagaa tgaaaggggg gacactttac atagaccaaa gtcttgatat
    48421 accccaaaca ggtgctgaaa ccgtcgtgat tccctttaga gaaaaggtgg gcgctcaaaa
    48481 tagctcgctg tacagcgttt tttacatggt tggctcccag cattttttcc ccatggaggc
    48541 tttaagggat atttttatgg ctaataaaat ttcgcaacgg gttagcgtgg atatctttat
    48601 gcagctataa ttcagaatga tgcaaagctt gttttggtgg aaataccttt aagttgtttc
    48661 cagtggtatg gtatgtcggg catatacaca ctactggcaa gatgcaagtt cttgaacacg
    48721 ccgggaatgg atgaagtgct agcgaagggc aggggtgttg ctgatggctg caaatctgga
    48781 aaaagccata agcaagggca gcgatgcagc atttaccctg caaaaagcca gcagaggcca
    48841 tagcagccta caagggctga atcttttgat gtagggagca gccggtacaa aagtaaatcc
    48901 ctatggagcg gaagcaagct atagcgcatc cgcaggagtt gtttttcaac agcagcggga
    48961 acgctatact ccagtgaagc cgtaagcggg gctgggggca gtaaagtttc agcaggaggg
    49021 tttagcagga gtacagcgac aagacttaac ccacggattt atgagaagtt acacgtttaa
    49081 aggaaccgcc gtgtccacct caggcggatg ccgggtggtg taagagggag ggggagtacc
    49141 cccctaccta accgattaat aaggtatgca gatcttgctt gcgcttttag tcgttattgt
    49201 ttagaaaccc aaggtagtag aaaagggtgg ccaacgagcc tagggctgca ataacatacg
    49261 tgagggctgc ccattttaga gcatcacgtg cgtaagctac attttgaccg tacagaattc
    49321 cggttgagcc tagccactgg agcgccctac gcgatgcatc gaactcaaca ggcaaagtaa
    49381 tcaggctaaa cagtgttgta agggccagca atgccacgcc tacaaccagt atggccggca
    49441 tagattgaag cagaatgact ccggcaagca aaatccattg aacccatcgt gaagctacac
    49501 ttacaacagg tacaagggac gatcgtagct gaagaaaggc gtagctttgt gcatgttgaa
    49561 ccgcgtggcc gcactcgtga gcggcaaccg cagcagcggc aatgttgtgt ccgttgtaca
    49621 ccgcggggct taaatttata gtcctgtcag ttgggttgta atggtcggtt agctgacccg
    49681 tggttgatat tacgcgcaca tcaaaaatac catgctgctt tagcatcatc tcggccacct
    49741 ccttgccggt cataccttgg gggaggtcaa tcttcgagta ccgtgcaaac cttaccttta
    49801 gctggttgga tatcaggaag ctaagcacga aaaaaataat cattatcagg tatatgccca
    49861 tggtgtttaa tttttatttt ggttagaatt tcatccttta cctacgttta tttactacta
    49921 aaaacagaaa gtggctcaac ggcaagtttc tcggccactt cgttaagatc gttgagaact
    49981 tgaggcaata ttgtaatacc ttcctttgat tttatcctct cgttttccct ttctggatca
    50041 ccggggatga gtactggcgg ctggtttacc ccgctttttg ctgcacggaa tgttttaatc
    50101 cattcgtcca tcattagctt aaactcgttg gctgggcgaa aggcatcaat acgaatagca
    50161 ccgaaaaaat gcccaagtcc tttgccaacg gaatgttcag gaatggggag gtagggaacc
    50221 tgtggaggca caaatgggcc aaagttggca cctgaaagta cggccgaaaa aatatcaact
    50281 attgaggtca ggcagtatcc cttgtggctt ccatgctcat agtctcctcc aagcggtagt
    50341 attccaccgc cgtgaagcaa ggtgtccgga tcgtcggtgg gctttccaag tccatcctga
    50401 acaagtccga taggtgcttt ttcgcccttt ttagccatta gagccagctt gccgcgggct
    50461 attggggctg tggcaaaatc ggccacaaag ggtggctgtt ctaatgctgg aatggccact
    50521 gctatgggat ttgttcctaa aagcttgctc gttgaaaagg ttggaactac caggggattg
    50581 gcattggtca tgctaatacc aatcatatcc tgctcaagcg ccatcatggc gtagtatcca
    50641 gcaattccat agtggttgct gttttgggtt gcaacccatc ccgttccggc aacttttgcc
    50701 ttttctattg ccaggcgcat ggagtaaagt cctgctacag gccccagtgc attatcacca
    50761 tcaactgtag cagttgatgg agtttcatgt atcgttttta cagatggcct ggtgtttatc
    50821 ctcccggcag ccaccatttg gtagtagtcc ttcacccgca ttactccatg tgatggaatt
    50881 cctcgaagtt ctgctgctac cagtaggtct gcaacggatt ttgcatccat ttccgaacaa
    50941 cccatccgct caaacagcag gctaatgtag ctcttaagtt taagataatc gatcataaat
    51001 ggcaatttat cttttacaaa gataatgaat gtaagtattt ttagaggtct agctattggt
    51061 tccgtatagc caactgtgta acaaaacaaa ccgtaataag cttactgtca aggtataata
    51121 ttttgttaag tgtttgccgg ttttttaatt taacaaaatt taaatcgctt tgaaaggtta
    51181 aacgtgcaat acactcaaaa aggcataatg gaaagcaaat cacaagcact atagaagttt
    51241 tcgatattac aacctgtagc tgtttgcggg cgttttttaa tatgattgat ggtgagtgga
    51301 tttgatgcaa aggatattag gttcaataca tctttgagca acacttgaag aatgtttata
    51361 ccctggctaa ccaagagaac atagctataa ttttttgatt aaaattgtgc gaaatttttt
    51421 gttatgacct ataatgaagc gttagactac atggtgtcgc agctacccat gtttcatcgc
    51481 gttggcaaag ctgcctacag ggcagacctg ggtaatgcgt tggtgctgga tgaatttttt
    51541 ggtcatcctc atcaaaagtt tgcatccatt catattgcgg gaaccaatgg aaagggcagc
    51601 acatcccaca tgctcgcatc ggttcttcag caagctggat acaaggttgg gctttacact
    51661 tctcctcacc ttaaggattt ccgtgagcga ataagaatca atggtgaaat gatttcacag
    51721 gttgaggtta ctcgattcgt ggaggagtat cgggcgcttt tcgacagggt aaaaccttcg
    51781 ttttttgaga tgactgttgc catggcattc gactacttcg cacgtcgaaa cgttgaggtt
    51841 gctgtggtgg aggtgggact tggtggaagg cttgattcaa ccaatatcat taccccaatc
    51901 ctttcggtta tcacgagcat tggtctcgac cacaccgatt tactgggaag tacacttcct
    51961 caaattgccg gggaaaaggc aggtataata aaaaggggta ttccggttgt cgtaagccaa
    52021 cttcagcccg aaattgctga tgtttttact agtaaggctg gtgaaatgca atcacctctt
    52081 gtttttgccg actcagaata taaagttcga gaaatgagct attccgcttc tggcacgcaa
    52141 acctttctgg tggaccatgg gggaaatcct gagcagtggg atacagatct catggggagc
    52201 taccagcgat taaatctacc aggggttctt gccgcgcttg atgttttaag gaatgaagga
    52261 tacaggatta catccgatgc ccagaagcag ggtctcagga tggtgcaaca gctcaccggc
    52321 ctgatgggtc gatggcaaaa acttcaggat aggccattaa tatactgtga tacaggacat
    52381 aacgtagatg gtataaccat ggtgatggag caaatccacc ggatgagcta cgctaagctt
    52441 catgttgttt tgggtatggt tgccgataag gacattgatg gcatgcttag cttactgcct
    52501 gccgatgcca tttactactt tacccgtgca gcccttcccc gtgctctgga ccagaatata
    52561 ttgaagacta aagccaaggg ttttggattg aaaggtgaag catatcctac cgttgcccag
    52621 gcccttgagg cagccaaggg aagtgctaat gaggacgatc ttatctatgt tggcggaagc
    52681 acttttgtag tggccgaagt ggtgtagtga tattttttca aaaaaaaatt tgctgattta
    52741 aaattttagg ctacctttgc tccgcatttc aaacgagagt gcagtcaaaa taaagtattg
    52801 ggagcttagc tcagctggtt tagagcacct gccctacaag cagggggtca taggttcgaa
    52861 ccctatagct cccacaaaaa aaaattaaac cgcatacatt ctttcaatgt aggcggtttt
    52921 tttattggat gatttttttt ttggttacgg aagggtattc tacaaaacgt ccagtacacg
    52981 ttcaagccca acaccccgcg aaccctttaa aagaatggtt gcatttgcca cttcgtggct
    53041 taggaagtac tcagcacatg ctttggcgtc tgcgaaccag ccgtagctgc tatttattcc
    53101 cgaaaagttt tgtccaatga gaaaaacctg tgttgcccca agcgattcaa gcagtttaac
    53161 cactttctta tgttcacttt cggcgtagct gcctagctcc agcatttcgc caaggataat
    53221 tatcttattg ggactctcaa gagaggtgaa gttgcgtacg gccgcctcca tgctggttgg
    53281 gttggcatta taggcatcca ttatcaccgt attgcgcttt gtttgtataa gctgtgaacg
    53341 gctgttagaa ggattatact cctcaatggc atttttaata agttccggct ttactccaaa
    53401 gtgaaggcca acagcaatag ctgcttcaat attttcaatg ttgtagcttc ccaccagctt
    53461 tgttttaatg tccatgggct tatctgtacc tgccatttca acattaacag aaatgaatgg
    53521 tgtatcatgc tggtttgtta cctttatgct tttacaatag ttggaatact ccagcttgtt
    53581 ggctatgccg attcccatgg caagctgttt aagcacttca ttgtgctggt tgtagataat
    53641 caaacccttg tgccgggcga tgtagtcata caactcgccc tttgtcctta ccactccttc
    53701 gtacgacccg aaaccttcga ggtgtgcctt tccaatgttg gtgattatcc cgatatcggg
    53761 tgctgctatt gaggctaact ctgcaatatc cccgggatgg ctagcaccca tttccacaat
    53821 ggcaacttct acatcagccg taagggaaag aagcgttagc ggaaccccaa tttggttatt
    53881 caaatttcct tttgtagcaa ccgcatggta tttttttgag agaacgcatt gaatcagctc
    53941 cttggtggtg gttttaccgt tggtcccggt tataccaatt attttggctt ttagttgttt
    54001 ccggtgatgg ttagcgagct cctgtagggt ttttagggtg ttttcaacca aaatgcaatc
    54061 atgcgtcata aaggttggat tgtcgattac agccaaggat gctccattat ttaatgcttg
    54121 ttctgcaaac ttattcccat caaatttgtc tcctttcaag ccaaaaaata ttgcaccttt
    54181 aggaatgctt cggctgtcgg tggcaactga gccatgattt aggtagtggt ggtagagttc
    54241 ttcaagcatg ggttgggttt ttatatcctg ttttttatta ttaagttgat taacttaggt
    54301 tctttactgg gtgtgtatgt ggttgatggt gatggtaaac ttttatagct tggttgtagg
    54361 gtaggggtaa tatacaaatg taaggcttgt caatggcatc ggaaattctt ttaaaggtta
    54421 taccttcaaa aagcagaaaa cctcatgttt atgaggtttt ctcgatatta attgaagtat
    54481 ttccaaaaaa aacgttactc gcctctttga cgggccgact gggggccaac agcctccatt
    54541 gcgcaacgaa atccgatatc gttggttgaa gaggcttcgt ccagatacct tctggtggcg
    54601 ggactcaacc agtaggctct atccttccag gaaccaccct tgtaaactct aacatggtcg
    54661 ttaatgaggc tggtcatgcc ggcacctttt gcatcaggtt taccctgatc gtacatggcg
    54721 ttagaatctt tggttttaag ggtttcccct tcgacatagt ttagagtaga agcaacgtcg
    54781 ccatctttat agttaatata gtaggccttg ttatagtttc gcctgttggc agcttcttcc
    54841 tcggtaacct ccctgtatgg aattctaccc aggctatcct tttttgcaaa gttgccttct
    54901 ttatcatagg gggttttgaa tacatttcca cgaaatggtc taatctcgtc aaagtcttcg
    54961 tagcttaaag gacggtaaac gtcgagcacc cactcgttga cattaccggc catgttgtaa
    55021 agcccgtaat cgttaggcca gtaggactta acaggaccag ggaaggtatt ctggtcgttt
    55081 aggtttccag caagtcccat gtagtcgccg cgtcccctga caaagtttgc catcatttgt
    55141 cccctgttct tagattcaga gtttctaacg ttgtggccat tccatgggta gatgcggcgc
    55201 tcaaccattc gttcttcgta ggtattccca actaatcctg aagctgcaaa ttcccactcg
    55261 gcttcggttg ggaggcggta ttttggcagc agaatgccat cttcaaagcg aaccggacgg
    55321 ccttcgggct gctcggggtt aaggcttggc aaattttcct taacttttcc cacatattga
    55381 ccggcaaggt aggcttcggt gttgaaaaca tcatcaccct tttgcccgag ttctatttca
    55441 agaattccct tttgaacaag cagcatttca tttacccgat cggtacgcca ctggcagtag
    55501 tcatttgcct gaagccaatt tacgccaaca acgggatagt cgttatatgc agggtgacgg
    55561 aagtaatcgg taacgaatgg ttcgttatag cccaatgggg atcgccaaac cagcgtatct
    55621 ggaagagcct gctttattac ctgcgggtag tccgaataaa cgcgtttaag ccagtatatg
    55681 tattcccgcc agtcaacgtt actaacctct gtttcatcca tgtagaaaga ggttacggtt
    55741 acccttctgg gcttggcatt ccaatcgtac attaagtcct gttcaacccg acccataatg
    55801 taggttccac cctcaatgaa caccaatcct gggccggctt ccggtttcca gtcggtaaca
    55861 acttcaaaac caccttcgtc gggcgaattg tatgcccatc cggtttttga tgatacgttt
    55921 gaccccccct taccagaaat tccacatgaa gtaagcccca acatggctac caaaataagg
    55981 cttaatggtt tattctttaa gttcataact atttattttt acccttaaaa taagttcaaa
    56041 tatagtgata aaaaattgtt taccacaatg tagtaacgta aaaaattaaa atttcggaca
    56101 tttaatgtac ttatatttac tttttccctt tttatactca aaattccatc ccagggttac
    56161 ctcatgcgat gaggttggta atcctctaag gccatacttc attatgctga agtcgtagct
    56221 atagcccatt ctaaacaaat cgtcggtgta cccgatcgtg aaaataaatg aatggctgtt
    56281 caggcttagg ttttccctta gccaaaatcc tcccaccaac cagtgccggg aaaggtagaa
    56341 cccaatgttt agctggttaa acttggcttg ttgaatgtat attatgttcg gtgacagcag
    56401 gagaacctct ttaaatttgt accatttata caggttgaag tcagcgccaa tatgaacggt
    56461 gtacttccgt ggtatgtaag cttttgtgtc ctggtaggtt gcctccacgg gctccagaag
    56521 gtggtgaaca gctattcctc cataaaaatt ttcaaaatcc cccgctactc caagactgaa
    56581 atcatagttc catgttgtta ttcccgaagt attataagta ccggttacat ctccatttat
    56641 atcgatcata tccggaaata caagccccga gtagttacgg gtacgcacaa tgccacttgt
    56701 ttgaatacca aaacgcaaat gtgagttata ggaaggctga atcccgtaag catagaccaa
    56761 atctaccgtg gtgcggctaa acactccctt gccctccaca tcggctacaa tattgagtcc
    56821 cacaccgctt tttatagatt gaattgtcct gtcaaagcta accccatagg tagagtaggg
    56881 catatcaatc atggtccatt gatttctgta aacaactcct agtcgcatgt atttgctagc
    56941 gccggcgtaa gcggggttta gataaagagg gttgaaaaat agctgggaaa atattggatc
    57001 ttgcccaaat acccttattg atgtaaccat tgttatacct atagcaacaa aaaattttct
    57061 aaacatttta ttatcagtag ctaaatattt tgttgataat aattatgaat cctttgccgt
    57121 ttttattgtt cttgtaactc gtaaaagtaa aataaagttt ttatttattt gcaacagcaa
    57181 aatcaatttg gtgatgtgca tgtttagaag aacgtttttt ctttcgcttt tgtgtatttc
    57241 aaatggcatt tttgcacagg atactctgga gcgtgcaatt tgctggaatt acaggcaagt
    57301 acccactgct caggctgagc agaccatact gtggttcgat aacgccgttt acacaaatcc
    57361 acagacaatg attccttctt actttgagct aattccaatc gagaataatg gtgcatacaa
    57421 ttcaacaccg gatgttttgg tggctgacga gcaatgggtt ttggtggcta atgacgaaat
    57481 gaacctgttg caaggcgtca ataactttat aaatgaaagt gttgactgca gaacagccat
    57541 tcaaagggga aggatgatga ttcaacttac tgttccggcg gtgaggctta attctgacaa
    57601 caagtttgag aagctggttt cttttaaaat tatactaaaa agggcagaaa aaacaaatga
    57661 tgaacgcagc tatgcccctg ctcgtctcca ggtcaaatcg tcggtgctaa gttccggtag
    57721 ctgggtaagg gttagcgttc gggagggtgg tgtatataaa atttcataca acgatcttca
    57781 atcttatggg ctgtcaaacc tcgaaaatgt tagcttatgg ggaaatggag gacactgtct
    57841 tccctatatg aatagcgagc aggcacctga cgatttaaat ccaattccta ttctcatgag
    57901 taagggtgat gacggaattt ttaaccaggg cgattacatc cttttttatg ccgaaggccc
    57961 caaaactatt tcctacaatg ctaccatgga catgtgggag gagaaaagcc atccttatgc
    58021 ccaacaaatt tcatacttca tcacaacttc tttgcctcaa tcaacaattg aaaccataga
    58081 agagtcagct gctccattaa accgcacggc taattcatat gatgcattct taaccattga
    58141 aagaaacgat accaacatgg ttaaatcggg tagggagtgg tttggcgatg ttttcgattt
    58201 aactacatcc cgaacatata ctaccgacct ttccctacct gttgtgggct tacctttaaa
    58261 agtttggctt agggcggcag cacgctcttc tgtagctagc aactttacgg taaaggccaa
    58321 cacaaccacc cttggaactt tgcctttaaa cagcgtagta attggtgatg agtacgccga
    58381 tgtggtagca gtgaatgagc aggtttacac ctctgcactt aacgaaggca acctgacaat
    58441 cgaattaacc tatagtaaac ctaattctac tgcaaccggc ctgttggatt ttatcacaat
    58501 aaatacccgc cagcagctta aatataatgg ttcacagctg attttcaggg ataaacaatc
    58561 ggtcggaagc ggtaatataa cggaattcag catacttaat tcaaaccaaa atgttcaggt
    58621 ttgggatatc actagtttca accatcccta caggctcaac tccaggtatt cgggaggagt
    58681 agtagcattt aaacaggcca ccgataccct acgccatttc attgcttttg aacctgaaaa
    58741 ggctaagacc gttacttttg ttggagctgt ggctaaccag aacattcatg gagctggaca
    58801 gcccgatttc gtgatagtag cccatccgct attcatgcca caggcacagg agctggctca
    58861 acttcaccgc tcacttgatg ggctggatgt tgtggttttt tcaactgatg aggtctataa
    58921 cgaattttcc tcagggaatc ccgatttgtc ggctattcgc aacatgatgc gatattttta
    58981 caggtgtgct gctacaaccg agcaacaacc caagtacctt cttttgtttg gcgatggatc
    59041 atacgataac ctatccgaca ggaatggcaa caccaactat attctaacct accagtcgcc
    59101 acgttcaatc aacaaagttg gttcgtttca gtccgacgac tactttgggc tgctcgatga
    59161 cgaagagggt gaagctgttg gcctgcttga tgttggagta ggccggttgc ctgttagcac
    59221 taccgagcag gctaatgctg ttttagcaaa aattcagcac tatgtaagcg gcagtaacac
    59281 tggtgactgg caaagtaagc tttgttttat aggtgatgat gaggatggaa atattcacat
    59341 gacggatgcc aacacccttg ctgattacct ttcaaccagt aatccagaat acaacattca
    59401 aaaaatattt tttgacgcct acaggcagga atcttcatcg gggggagcta gctacccgga
    59461 ggtaaccaac gctataaaca caacggtgaa caacggggta ttccttgtaa actacacggg
    59521 gcatgggaac gaaaggtggc ttgcccacga gaaagtgctg atgctatctg atgtatcatc
    59581 atggaaaaac acaaaacagc tgcctctttt tgtaacggcc acctgcgagt ttagccggtt
    59641 cgacgattac cacctcacct caaccggtga gcagattcta ctttcgcctg acggtggtgg
    59701 tattgccctg gtttcaacta cacgtctggt ttactccagc cctaacttta ctttaaatta
    59761 taaatttatc aacaatcttt tcaagtttaa tgaggactca acctcccatt accgcttagg
    59821 cgacctcatc aaaatatcca aaaacctttc cggaacgggt aacaacaagc gcaactttac
    59881 cctgctgggc gatccggctt taatgcttcg ataccccgaa cttggagctg aaattaccag
    59941 cattaatgga aaacctgttg atgaggaggc cgataccctc aaagctttat cggttgcaaa
    60001 cctctcggga aggatattta ccccgattgg ccaggttgca tcagacttta gcggaaatgc
    60061 caccataact gtttttgata agaaaagcga aattaaaacg ctggcaaatg atggcgggat
    60121 gccaatgacc ttttttagtt gggacaatgt gctctataaa ggcagagcta ttgtcaataa
    60181 cggattattc agcattgatt ttgttgtacc aaaggatatt cgcctatcgt ttggcacggg
    60241 taggataagt atttttgctg acgacttggc aagggtggca atgggctaca acgaatcggt
    60301 aattgttggc ggggttttca actcacctta taccgataac gaaggaccgg agattgacgt
    60361 ttaccttaac gatacacttt tcaacggggg aggaatatcc gatagcagcc ctaagctgct
    60421 tatccggtta agggacgaga gtggtattaa tacttccgga attgggattg gacacgattt
    60481 aacggcaacc cttacttcgc ctggtggcga tgaggttaac gtaattctaa acatgtacta
    60541 ccagtccgaa cctaacagct accaggaggg gtatgttaac taccagctag ctgacctttc
    60601 cgaaggaaga tactggctta gggtcaaggc atgggatacc tataataata gctctgaaaa
    60661 aattatttcg ttcgttgtgg ttggagcagg aaaccttcag ctggaaaaat tctactctta
    60721 ccctaaccca ttttctgagg gaacatcttt ctactttgaa cataacatgc cggaagatga
    60781 gttcaacctt acaattcaaa tttttgacgt ggcaggacgg ctggttagca caattacaca
    60841 gtcacaggct tactctgggg gataccgggt tggacctatt tactggaatg gaactgacag
    60901 caggggaagt cggcttggaa ggggcatata catctgccgc ttatccgtta aaacttctag
    60961 cggatatgca gccagcttac agcataaact tgtcattttg aggtaaaata taattccgag
    61021 ttttacgttc gctagaatat atttcacaat cagctaaatt tgcacctaaa atttatatca
    61081 gatgcgtaga atactgttta cacttctttc ttttgttggc ttcatttttt tttcacaagt
    61141 ctcaagtgca cagttcgata gcactgtttc gcttaccggc ggactgaacg ctctcaagat
    61201 tgccgttcct tttctaacta ttgcacccga ttcaagagct ggcggaatgg gcgatgctgg
    61261 tgttgccact tcacccgata tcaactccca gcactggaat ccggctaagt tcccatttat
    61321 cgaaagtaaa aaatggggtg ttgccttttc ctatacccct tggcttagaa atttggtgaa
    61381 tgatatcaac cttacctacc tcaccggtta ctaccaggtg gacaagatgc aggccatcag
    61441 cagctcgctg ctatacttcg ccatgggcga aatccagttt actaccgaca accccaatga
    61501 ccccccggtt aaatttttta accctaacga gtttgccatt gacattgctt actcccgcaa
    61561 attttccgat tacatttcgg gtgctattgc cttcaggtac atccgttccg acttgagcgg
    61621 tggctactcg caacccgggc aagcctcggc taaagctgga acttcctttg cagccgatat
    61681 tgctatgtac tatcaacatc ctgtaaagct aagcgataaa tttgggcaaa tggcttttgg
    61741 tttcaacatc tccaacatcg gcacaaaact ttcatacagc gatgacccca tgaaggattt
    61801 tataccgatt aacttgcggc ttggaggaag gttgaccatg gatatcgaca gctacaactc
    61861 attctccttt actgtagatg ccaataaact actggttcca actccacccg tttacggagt
    61921 tgtagatgag caaacagtaa tagttaaggg gatgaacccc aacgtgtccg tcgtacaggg
    61981 aatgctacaa tcgttctacg atgcccctgg tggttttaaa gaggagctga acgaaattta
    62041 ctatgctaca ggcattgagt actggtatgc ccgtcagttt gcccttagag cagggtattt
    62101 ttatgagaat gaaaataagg gtaaccgtaa gtactttact gttggtgcag ggctgaagta
    62161 caacatcttt aatattgatt tttcctacct tattccgacc ggtggattta acagcccaat
    62221 ggcaaacacg gttaggtttt cgctatcctt tgactttgag gctacacgta tgcgtggcca
    62281 aaaagtcaac aacaactagc ccatgaaagt tcgagtggga tttggctatg atgttcataa
    62341 gctggagagc aacacccctt tccacctggg tggaatattt atcgagcatt ctaaaggagc
    62401 agtaggccat tctgatgctg acgtgcttat ccatgctatt tgtgatgcca tgctaggcgc
    62461 agcaaacctc agggacattg gctaccattt ttccgacact aaccccaact taaagggtat
    62521 tgatagcaaa attttgcttg ctgaaaccct aaaactactt gatagcaagg gtttcagggt
    62581 agttaacatt gattcggtag tgtgtctcca acgccctaag cttatggaca tcattcccaa
    62641 catgcagcta accctggcat ccgtccttaa aatggacatg gaggatattt ctattaaggc
    62701 cacaaccacc gaaaagttgg gatttgttgg aaatgaagag ggcgttactg cctatgccgt
    62761 tgtcctgatt caaaaggatt gacccacatg gtacctgaaa atgcagaaac gttttctacc
    62821 cttgttctgg gggccagctg taaagcaggg aggttttcct atctggccat taaaagtctt
    62881 gtgaaacacg gggttagggt ctatgcaatt ggctcgaggc catgctttgt tgacggagtt
    62941 caagtacatg cagaaaaaat taacctcacc aacctccata cggtgaccct ttacctgaac
    63001 agtcaaaatc agacaaccta ctacgactat attttaagcc taaaacccaa acggattatt
    63061 tttaatcccg gtgctgaaaa ccccgagctg gtgagattag cacatgaaaa cagaatagac
    63121 tatgtttttg gatgcacctt agttatgctt aacgatggga gtttttttaa atcatagatt
    63181 cccattaaac attctgcagc aaccctgctg gcaaattcca tgctactaat tattaaaatt
    63241 gataaataac ttaatttcag ctatttacga atagcacttg ccaaggagaa aagccatgtt
    63301 tttaaaattt tgttaaaata tttatgtttt tttaatacat aagcttaaaa cttatgtaaa
    63361 tttgcatcgg taaataacaa ttaccaatag atatttaact tcattaaccc aaacaattcg
    63421 attatgaaaa agacagtttt actaggattc gcagccttaa gcatggttgc agttctttct
    63481 ggttgtggca aggtgcccca ggagaaaatt gatgctgcca atgcagctat tgactctgcc
    63541 aaaatggttg aagctgatgt atatgttgca gccgattttg cagcagttca agattcgatg
    63601 agcgctgtta tggccgacat cgaagttcaa aaatccaaac ttttcaagaa atttggacct
    63661 gctaacgaaa agcttgacag aattctgagc caggctcatc agattcaagc taacgccgtt
    63721 accaagaagc aggaagttaa ggtagaagtt gaaggtttaa tgaccgaagt aaccacctta
    63781 atcgaccagg acaaaacttt gatgaaaaaa gctcctcgtg gaaaggaagg cgctgctgtt
    63841 cttgaacaaa tcaaaaccga aattgccgct atcgaagcta gcgttgctga agccaaaaca
    63901 atgtacgaca atggaaagta catggatgct tccaacaagc tgaaagctgc taaggatagc
    63961 gctactaaaa tcaacaccga acttaccgat gctattgcta aggtaaaaaa gagatagtat
    64021 aattcgattt tccgaaaccc cggggttgct aaacttcggg gttttttata ggctattctt
    64081 atttagttac catgttttaa ttttggacga ggattttcac ctcaacgaat atgctagtaa
    64141 tattgataga ttagccaatg tatgctaatt ttgcatactg taactgatgg tttttacata
    64201 gtggcttgtt catgttgctc ggaaatagtt ggtttactta ggttttataa atcatttttc
    64261 tataacctaa tttattcgtt atcagcctat taaataggag tttatgagta gaaaatcgaa
    64321 aagaatagct gttattatac tggcgagttt ggtcgtggct accggcattt ctgtgctatt
    64381 ctacaagtta atacctgaac ctccgattgg agatgttgac tatgcccgtt tatgcctgca
    64441 gcaagcctcc aagagtaaag cacagagcta ctctaaaaaa agttttaaac aagccaaggc
    64501 cgactacgat tctgccatga agtgctggca aaaggaaaat gaaaagttta tcctgttccg
    64561 tgactattcc atggttgcaa aacttgccca aaaatcggcg aaagaggcaa gaaatgccat
    64621 gaacaattcc caaaacttaa ggaagaacat ggttgtagct gttaaggata aaattgaact
    64681 cctgaatggc ctgatactaa atgtaaatga gcttttcaac agggtgccac ttccccagga
    64741 ggtaagggat aagatttcac gggggaagct gctgcttaag gaagcccagc tatattttga
    64801 aaggggtgag tacctgtcag cccaggacaa aattgttctt gcagaagata tgctcatgtc
    64861 atcctacaat agaacacttg acgacttgga agagtacttt aactcccaac catactggcg
    64921 taagctggta aatagcacca ttgagaactc tgcaaaaacc aacggaaacg ccattattat
    64981 tgataagttt gcccgaaaac tttatatcta ccagtcgggt gtgaaaaaac ttgagtttga
    65041 agctgagttt ggtaaaaact ggatgggtag aaaaaggctt aaaggcgata aggcaacccc
    65101 tgagggtatt taccttgtaa aaaccaaaat cccccagaat aaaacgaagt actacagggc
    65161 tcttctctta aactacccga acaatgacga tgttgaaatg tttagaaggg aaaagcagca
    65221 aggtaaactt cctgcaaatg ctcaaatcgg cggcttaatt gaaattcatg gtaacggcgg
    65281 taagggaaca gactggactg acggatgcat tgctcttagt gataatgata tgcttaaggt
    65341 ttacaacttt attcaggttg gaacaccggt tgttattgta ggctctttag ccgattttaa
    65401 aagcattacc gaaaatttca agttgagaaa acatacagaa aggagctaat tatatatgga
    65461 tgccgtaaat atagcccttg ccactaccga taaagacaaa tcgattggaa aagtatcaaa
    65521 atttaacaat atcctagccg gactgttaat tgtgattctt tcaattatcc ttttttttgc
    65581 catacttatt tacgctattc ccccattaac cgagctggta atgaaggtta atggttttgc
    65641 tccccccagc tctgagattt tttcacccac acctaaggat tatgccaaaa tcaaactttt
    65701 gaccggcaaa atgcagcaaa aatatgttgc tttaacaccc ccaaggccat acattgttgt
    65761 taataccacc tataataggt ttaaactata taagaatagg aggttaattc gtgagggatt
    65821 ctgcagcact ggaagctata cactcctcaa atcgcacttt aatcgggaat ggatttttca
    65881 aacccccaag gggttttacc gaattcaagg aaagataacg aatcctgttt ggcggaggcc
    65941 ggactggtcg tttgtggaag aggggcttcc aattccttcc cagaacgatc cttcccgatg
    66001 ggaagcagga gttttaggcg attacgccat gagcattggt gatggctacc tcattcacgg
    66061 aactatctat aagcgttttc ttggaatgcc tgttacgcat gggtgcatcc gaatgaacga
    66121 tgatgacctg gaagccgtat ataaaaccct cgaactaggc tcaaaggttt atatctacta
    66181 atagctgatc taatgcaaaa aaaattcctt aaaatttttc ttctggcttg ccttcccatc
    66241 ttcgctttgg ccctggttta ctttatacta aaattggttg ctgttaacgg agcggttgat
    66301 cgcttccaaa aattttactc gcagctagat ggagatgata agagtaatgc tgtttttcag
    66361 cttaccaacg attctgccta taatgctgag cagaacagct acgcctacta ccatgcccaa
    66421 cttgaggcgg caaattccga ttccgttgtg ctttgcatta acttcccgga tagcactgct
    66481 acccttttaa tcaagggtgt tacggttcat accgcaaaaa tcgaccgcta tcgggtaagt
    66541 aattttttta ctaaaataga tcaacgaatc ctctacaaag ctctttcata cccatttagg
    66601 gttgaacgcc aaacatcctc aatcccaaag gttcccctaa tgattaaaat tgcccccaag
    66661 gatacttcag aatatacgcc cgatatcgtc cccgatacta ccgacgttaa ccctgtctat
    66721 ttcttgctgg aagctaagcc cggcatgtta gtttattttt acgagactcc aaaaaatcaa
    66781 tcccggatta gcttcaacgg ggtgattact gatattaaag atagaactgc tattctgttg
    66841 gatgatctac agagaatagt acacttccga aagctggatt acaagccatc tatcacgcta
    66901 aggttgaaca ggaaggatgc aaaaattatc ttcagggcta ttcctaatga tgcccttatt
    66961 attttaaagc tatcgtaggg ggaaaaatgc cctgcaaact tgaaagcagg ctttgtaact
    67021 aatcaaaatc tttaagagag aattttttat caggttgttt gatagtaaca atagctaagg
    67081 aagaaaattt aaatcccctg agttcttggt cggggtggtg agattcgaac tcacgacctc
    67141 tacgtcccga acgtagcgcg ctaaccgggc tgcgctacac cccgattgtt tacctcttta
    67201 gaacaggcct actagaaaat tgattgtgaa aggttaatcc ttttttaaat agctttaaac
    67261 ccccaccaat ccatgcaaag gtatttattt tttgtatcct aaatgccttc ggttaatttt
    67321 ttgcctgttt tttttgtaac ttgttgccat aagaagcagc ttgatactat ggttgagtct
    67381 tttattcatt tcttatggca gtataaatca tttgcctttt cgggcttaaa tacttcagac
    67441 aactgccagc ttgaggtgtt agaccccggt acatataatt ctgatgccgg tcccgatttt
    67501 tttaatgcaa aggttaggtt gaacggaaca acctgggtag gaaatgttga gatgcacatt
    67561 aattcttccg aatggtttgc ccataaccac cacactgata gagcctacga taacgttgtg
    67621 ctgcacgtgg ttgttaacaa cgacaagccg gctataaatt cccgcggtgt gcctattccc
    67681 actcttgcca taagctaccc gttcgggttg gaaagagaat attcaagatt gacgagttct
    67741 ggaagctaca tcccatgcgg tgaggtgcta ccaaatgttg ataacctaac aaaaagagcc
    67801 ctcctcgaca gaatgatggt ggagcgttta caggccaaaa ccgatgaaat aggtaaatcg
    67861 ctcgaaaaat gtcatggaag ctgggaggaa gctttctatc gggcggtcac cagaaccttt
    67921 ggccttaagg tcaacgcttt tccggccgaa atcttagccc aggaaacccc tttaaaggtg
    67981 ctggcaaagc ataagaataa cctttttcaa ctcgaatcgc ttatatttgg gcaggccgga
    68041 ttactaaacc gttttgatgt agaggacgag tacctacttt cgctcaggat ggagtactcc
    68101 tttcttaaga aaaaatttaa ccttactccg atcgatggta gcttatggaa atttttacga
    68161 atgaggcctg tttcatttcc cactatccgc cttgcgcagc ttgccatgtt aatacatcat
    68221 tctacctctc ttttttccaa agcggtggag gcaagctcct taaagcagct gcaagaactt
    68281 ttccaagttg aaacatcgcc ttactggtat aatcattact tatttggatt tccggttaaa
    68341 tcacgaaaaa aaagtttggg cactgctact atcaacgtga ttattattaa tgccgtcatt
    68401 cccttcatgt ttgcctatgg tggttggagg ggtaaccaga aactaaagga aaaagcaatt
    68461 tcaattttgg aggagattcc ccccgaggaa aatcatatta taaaaaaatt cgattctatg
    68521 ggggttaagt catgtaattc gtctgatagc caggctgtta tacacttaaa gtcaacattc
    68581 tgcgatgctc gaaagtgcat attttgccct gttggcataa aattattgct taaagcagcc
    68641 tatatttaga gtcttaaaac gaacgtaaaa atacacccta caacaaactt tataacattt
    68701 gcggaaatcg gggaattgct ggagttaaag aaaatacacg tatttaattg caatatgaga
    68761 atactggtta ctggggcaaa cggacttgtt gggaaaaaaa ttacccggca gctactttca
    68821 ggcggaaagc atgaggttta tgctacatcc ctaaaaaagc tgcaaattcc cggcgctgtt
    68881 acctttacat caaacctgct aaatgcagac attaacttta tggttgatga aacccatccc
    68941 gatgttatta tacattgtgc tgcactttcc agtcccgatg catgtgaagt tgatcgtttt
    69001 gcctgtaaaa ggctaaatgt agatctcacc gcccgaatag ccatggcttg caatgactat
    69061 ggagttcaac ttattctgat gtccaccgat tttgtttttg acggattgaa aggtcaatat
    69121 gcggaagagg atcgggctaa tccaatctgc tactacggga tgtcaaaagc tgaggccgag
    69181 caggtggtag cgtcaatgtc agcaccatgg gctatcgcta gaacctcact ggtattcgga
    69241 tacgagcaaa ggctgtcccg gtcaaacatt gttttacgtg ttgccgagaa cttgataaag
    69301 ggtaacccat ttagggttgc tttcgatcaa attcgaacgc caacccttgc tgaggatttg
    69361 gctcgaggta tcgaggctat agctaccaac agggcaacag gaatatttca ccttgcagga
    69421 agcgagataa tctcagtcgc tgatttggcg gctaggacgg ctgaagtatt tggattgaat
    69481 ccagaacttc ttttgccagt gagcactgaa atgttaactg agccagccag gagacccctt
    69541 aacacttcgt tatgttgtca taaggcaatt gagcagctgg gctattcccc taagggttta
    69601 accgaggctc taaggttggt ttactcgcaa atgaaattag atgggtataa tccataatgc
    69661 gcttgatgcg ttgaatctct ggtagaaatt tgagcagcgg tttcaagaaa tttgcccatg
    69721 caaccttaat ataacacttg atacactcag ccagcgttat tgccggtttc taccatttta
    69781 tatttccggc aattaaaacc tgaatttttt atttgtaaac atgtatgcat acccccttag
    69841 ccggcatatc ccatttgttc tgttgagatt tgttaatttt gtaagtttct acctttatta
    69901 ccatgaagca cccatgtaag aaagcttgcc taagtatgtt tgtacaagcg ggggttacct
    69961 acgagctcgg cgaattcaaa atgtggatgt ttttttcgac caaccctaat gactagaagg
    70021 tagggaataa agtatttata atcagaaaca taaattttgt attgatgaaa aaaatttcgg
    70081 ttaacattca gtcggaaatt ggtgaacttg agggagttat tatccacacg ccgggcccgg
    70141 aagtagagaa tatgacaccc gaaaatgccg aacgagccct ctatagcgac attctcaacc
    70201 ttagtgttgc tcgaaaggag catgcccagc ttagcggtgt tctttcaaaa gttactcaaa
    70261 cctttgaggt tggcgatttg ttggaacaag tgctgtcgaa tccatctgcc aaggaggaat
    70321 tgattaggag aatttgcctt accgagaatg ccctgtggct aatggacgat cttctggctg
    70381 ttagtccgaa ggagttggga aaatttctta ttgagggggt tccattggta aggaataacc
    70441 tgaccaactt tttgagccac gaacgctttt ccctaaagcc cctttacaac ctttacttca
    70501 cccgcgacgc ttcatttgcc atatccagaa gcgtagtcat agccaaaatg gcaagcacgg
    70561 ttaggcatcg cgaaagcata ataatggagg caatcttttc aatgtcggat atttttgata
    70621 cctcaacggt taatccttct gcttttacac ttgcaagcaa cgctaccatt gagggtggcg
    70681 atgtgctggt tgttagggat gatgttttac tcattggtaa cggtgcccga accaacactc
    70741 atggtattga tttcattttg aataggttac aattaaacag tgatgctagg aaaaagtata
    70801 ttcttgttca ggagctaccc tataagcccg agtcctttat tcatctcgat atggttttta
    70861 ctttactgga tgcagataag tgtatggttt acgatccaat tattattcag cccaaccggt
    70921 accaaacagt ccaaattacc attgcgaatg gaagggttga ggaaataaaa acggttgaca
    70981 acctgttggt tgcgctaaag aagttgggaa tggacctcaa gccggtttac tgcggaggca
    71041 ccaccgacag gtgggttcag gagcgggagc aatggcatag cggggctaat tttgtagcca
    71101 tagggcccgg caaggtaatt ggctacgctc gcaacgtaca caccattgag gagatgaaca
    71161 aaaacgggtt tgaggttatt agggctaaag atgtaattgc tggaagggtt gatctaggca
    71221 gttactccag ctatgtagta accatcgatg gaagcgaact tccacgaggt gggggtggag
    71281 ccaggtgtat gacaatgcct gtgcggcgca aaaaagttaa ctggtaggat tccgtttaaa
    71341 aagcctgctt ttcaaccatc tcctttacca aattggctgc ttcctgaaac gatgttgcag
    71401 tatataccct taatcctgac tgatcgataa gctttcggcc ttccaaggca tttgttcctt
    71461 gaagccttac tattaccgga actgggatat tgcctatttc cctgtaggcc tccacaactc
    71521 cctgggctac cctgtcgcaa cgaactattc cgccaaaaat gtttatcagg ataaccctta
    71581 cgttgggatc cttaagtatg attctaaacc ccgcagcaac ggtttttgca ttagcactgc
    71641 cacccacatc cagaaaattt gccggttccc ctcctgaaag tttaataata tccatagttg
    71701 ccatggccag cccagcccca ttcaccatgc agccaacgtt accatcgagt ttaacaaagt
    71761 taaggttatt ctttcctgct tcaacttcgg caggttcctc ctcgtcaaga tcgcgttgcc
    71821 ctgctatatc gggatgacgg aaaagggcgc tatcatcaac tgtacatttg gcatcggctg
    71881 catatatatc atcgtcggat gttttgacta cagggtttat ctcgagtaga gaaaaatcgt
    71941 tggcttcata ggtgttataa agattcgcta aaaatttcac catctgggag tatgcttttc
    72001 cggttaggcc aaggttaaag gcaattttac gggcttggta tggttggaga cccagagggt
    72061 ttatttcctc cttgaatatg agttgggggc tttgtgaggc aactgcctct atgtccattc
    72121 ctccctgggg cgagtaaacc accatgtttc ggctggttga tctatccaat agtatgctta
    72181 tgtaaaactc ctttacctcc gctggccctg gctggtagat gccctgttct accaatacct
    72241 ttccaaccag tttgccctta ggccctgttt gatgcgtaac tagcctcatc ccaatgatgt
    72301 cgtgtgcata tcttcttacc tcctcggttg atttggcaat ctttacccca cctcccttac
    72361 ctcttccacc tgcatgtatt tgggccttaa ctgctataac atctgttcct gtggcttcta
    72421 tcacactttc agctacctta acagcttcgt cgggcgtaaa tgccacacta cccaatggta
    72481 cattaactcc cgatttcttt agtaatgcct tagactggta ctcgtgtagg ttcatactcg
    72541 gctttctatt ttgatttaag ttgttaaata taaaaaaaat gattgagtaa ttttgtcgaa
    72601 aattcaactt atggctaaaa acagaaaact ttccaacgaa gagcttcaca ggaaaactgt
    72661 tgacgagtat aaaatggttg aaaagtttcc tattgtcgtt gttctggaca atgtcaggag
    72721 cctaaataat attgggtcgg tttttagaac ttccgatgcc ttgatggttg agagggtagc
    72781 cctttgtgga atatgcggaa aaccaccaca tcccgaaatt catcgtactg ctcttggagc
    72841 cgaaaattct gttttatggg agtattttga taaaactgaa atggctattg agaagttgag
    72901 aggggaggga taccgcatca tctcttttga gcaaaccgag cattctgtca acttagttga
    72961 ctatatgccc atgccaggag tgaaatatgc tttagtattt ggcaatgagc ttcgtggggt
    73021 agggcaggat attgttgacc tttcagatga atgcgtagag ataccgcagt ttggcaccaa
    73081 gcattcgttc aacatttcta taactgctgc catagttctt tgggacttct tcgctaagct
    73141 taagaagcat taaggcagag cggcaaacca acaaccgtag caccaataaa aaaacctgcc
    73201 aacggcaggt tcttttataa actttttact gttcagctat tcagcaatag ccttgaaagc
    73261 ttcgttctgg aagaagttac gaaattcaac atcatccttt gcacgagcct tcaacgaggc
    73321 atccttgcca atagcggtgc gtaggttatc aagtgtggta gcttcctcct gcatgcgggc
    73381 acctacaata gcacggccat aataaccctt agctgtttca acctttgcaa aagtattctt
    73441 tgctgcatca acctttccgc taagaagcag ggcaagtccc tggttgaacg agtcagttcc
    73501 gccaaggtat tcagcagcag caggatactg acctttaaga atggctatta taccaaggtt
    73561 gtacttaacc tcgttaccgg cgtcggcagc atcagcaaat agtttttcag cagcttcata
    73621 gttcccctcc atcagcgcaa tgcaaccacg gttgttggta acggcaggaa ttttctgatc
    73681 ggcaggaatt ttatccaggg cagcctttgc agcagcagtg ttcttgttga ggatatagta
    73741 gtatgcagca ttgttataac cgcgagcgca gttgaacttt tcgcctgcct tcatgtaaac
    73801 ctcaatcttt ttatcaacgt tatctacaag ggttgctgcg taaagcattt cctcaacatt
    73861 aagtgaatcg taagcattgt tctcaaccat atactttaac gtatcgtcag agtagcctac
    73921 cttgtcaacc ttggcaatca ttctcgaacg acgaagttca gggagtacct tttcggccag
    73981 caccttgtaa accttcgata gatttttaat ttctttctcg cgaacctcag gatcggagta
    74041 catggaaaga actcgaagaa ttaagtcctt atcttcaatg ttcgaggcct gaacttgctt
    74101 ctggaaccct tcccagtctt cagaagtggt ttcaacgctt accgtagcgg gatttgttac
    74161 cttacccttt ttgaacacat cggcgaggta tgaatcggtg ttcttcccac gttccttcga
    74221 aagcttatcg ttaagagttt ctggtccatc gggcgaagca taggctgata ctttaatctc
    74281 tttaagctgc ttgttgctag ttttggcagc atcgagtaga aaatcgttta gcattttaac
    74341 ctcatccttg gtaagttcgg atttgcgaat atccgacttt tggattacga agttgatctg
    74401 agcttctttt tcctcggggg taacccttac aaatttatcc ttcagcataa ctggcttggg
    74461 gtctaggtca accagcttgt aggttgccat ggttccaata ccaattttct gaggagcatc
    74521 aaaaggaatc tgcttgtcct tgtaggtgat tacaaagcgc agctccattt cggcacgaaa
    74581 catttcgtcc ttgtagtcaa attcaacatc atgcttccat gaaccgcctg cttcgtagtt
    74641 aattacctgg ttgttggcct gcacatcttc cccctgaaga attttggaag gaagagccag
    74701 ctcaccaccg ttataaacta gtacaggggt aatttccagt gtggcttttt tgttgaaata
    74761 ctttgcggga actgtaccct caatagtggc ctttaccttg ttggcgtgaa cctcaagtgg
    74821 gtttggggta accttaaagc tggtgccctg gggtaattct ttcatctttt cggcaccgcc
    74881 gcatcctgcc agaacaactc ctgccagtgc tacgattgca aattttaatc ctttcatcat
    74941 tgtatcaaat ttggttaata gttaataaca tcgatttttt cagtaaatca tctacaaact
    75001 tagaaatttt ttttacaaat aaaaaatatg ccctaaaaat acatttatcc taaaatgtaa
    75061 gccggttaac accatttgga taggttttgt caaaaattgc acttttaatt ttattaaaat
    75121 cgtgctgatt tgcaacaaaa tccaactttg aggtttcgat gattagaaag gtgtagtcag
    75181 ggtgctgctt gaaaaaatcg aagtacccct gtgctatgct ttcgaggtac tcgccggtaa
    75241 tggattgctc atactctctg ccgcgctttt ttatatttcg tagtagcatc tgaacgggaa
    75301 gatgcaggta aacgtaaaga tttggcttgg gaagggtgtt gtatatgatt tggaaaattt
    75361 gcttgtagag ttgaagttca tcacccgaaa gggtgttctg ggcaaaaatg agcgacttca
    75421 taaagtagta gtcggcaatg gtaagactat ggaataatga acccattcgt agctcggcat
    75481 tcagctgcct gtagcgttcg gccagaaacg atagctccag agcaaagcta tacctttcag
    75541 gattttcgta aaatttaggt aaaaatgggt tatcggcaaa ttgctctgta atgagattgg
    75601 catttaaatc gtttgccaac atggtggcca gggtagtttt ccctgcacca atattccctt
    75661 ctattacaag gtattgcata ttcctacttg cttatttata actacttggc taagttagca
    75721 tattctgttc cgcaattcgt catcaataat tgtcactatg tttcggaatt taccgttgtt
    75781 gtacagcaat atgaatgggg gataagctgt tgctcaaccc ccatggttaa aaaagtattc
    75841 attcataaaa gagctagatg gatagcccca ggttgtaaag tataaaagcg taaatgtcgg
    75901 tgtactcgtc aatgatcagg gatgttggtg ttccagcacc atggcctgca cgggtttgaa
    75961 ttctgataag tgcaggattc tgcgggttgg gatttttttc ctgcatggtg gcaatgtact
    76021 tgaacgagtg cgctggaact accctgtcgt catggtcggc cgttgttacc aatatggctg
    76081 ggtattttgt gtttggttta atgttgtgca gcggggagta gctgtagaga ttctgaaact
    76141 gaatggaatc ctcgcttgat ccatagtcgt taacccatgc ccagcctatg gtaaatttgt
    76201 gaaaacgcag catatccatc actccaactt ccgggatggc tactttaaat aaatcgggac
    76261 gctggttaat aaccgctcct atcaggaggc caccatttga cccaccctgt atgaccagct
    76321 ttttgggtga ggtatacttt tccttaataa ggtattcagc agctgctata aagtcgtcaa
    76381 acacattttg cttgttgagt tttgttccgg cacggtacca gttttcacca tattcacccc
    76441 cgccacgaat attcgctagg gcaaaaacac cgccattttc gagccagaac aatctcgatg
    76501 tgctaaaacc cggcatcatg ctaatgttga aacctccata gccatataaa agggttgggt
    76561 tgctaccgtc aagtttgatt ccttttttgt gaacaatgaa catgggcaca attgtaccgt
    76621 ccttgctggt gtagaaaacc tgcttggttt cgtaatcatc aaagttgaat ttgatatcgg
    76681 gcttgaaaaa ttcggttgat tcatttttgg caacgttgaa cttgtaaaca gccggaggaa
    76741 cagtaaacga ggagaagctg tagaaggcca acgtgtcgct ataggagccg ctgaaaccgg
    76801 aaattgttcc gatggttggt aggtgaattt catcggtttt ttcaccactg agggtgtaaa
    76861 cctcggcctt gctcttggca tctaccacgt agttagcgat aattttgcca cccactagct
    76921 ggcaggaggt aagcacatcc ttacgttcag gcaggatgtc tttccagttt ccaacatcca
    76981 tgctgttaat gttgatttta ataagcttgt attttggagc tttgtagttg gttagaacgt
    77041 aaaggttgtc gtcaacattt tccaacacgc tgtactcata ctcgaaacca gttgtgagtt
    77101 gagagaattc cccgcgcttg tcggtgaggt ttttaatgta gatggagtta ccgtaggtcg
    77161 attcagtttc gtagataatc aggtattttt cgttctcggt agtttgggcg ctatagttcc
    77221 ttaggggatg ctgactgttt gcaaaaatca gcttgtcttc atttgaatcg gtccccatgg
    77281 tgtggtagta aactttatgg tactggttaa gggttgagag cgcttcacct tttttaggct
    77341 cgtcgtaacg gctgtagtaa aaaccgttgg cataccacgc aattgacgag aacttaaccc
    77401 aggaaatttc gtctttcagc tcttttcctt caggtaacgc aatcacttta atcttttgcc
    77461 agtcagaacc tccgtcgttc actgcatagg ccaggtattt accatcgcgc gatgggctta
    77521 cttccgaaag ggctatggta ccatccgcac taaaggtatt ggggtcaagt agaactcttg
    77581 gttcagagtt tagagtttcc ataacgtaga gcacactctg gttctgccgt ccatccttct
    77641 tgaaaaagaa gtatcgcccc ccttccttaa agggaacccc catggtggta taattccaga
    77701 tgccggttag cctttcccga atcttctcac ggaatggaat tttagcaaga tacgatgagg
    77761 ttagctcgtt ctgcttcgaa acccactcgg ctacctcagg cgaattttca tcttccaacc
    77821 agcggtaggg atcgggaacc ttagttccaa agtagttgtc tactacatca acccttttgg
    77881 tttcgggata ctgaatccga tttttttgac aagcgtaaaa cagcatgggt attgttactg
    77941 caagtatgat taattttttt tccacggata caataattta gttgaacata tttgaatgta
    78001 atcgtattgg catcaaacta cctgaataca ttgtagtgtt aataggatat taaaatgact
    78061 acctttatac tagcccaaaa ttatattaaa ttcatcagct tctcatagat ttgtcttcaa
    78121 gttaactttc aagaactcta cccggggcat aaaacttcat cccaatcagg ctttccataa
    78181 gaaattgaag cggttaaagg atatataaag ttagccatgt ttaagtacgc ctatgttatt
    78241 caacaaaagt ataatatttt taacaaagta ttattattga gcaccattga ttaaagtatt
    78301 ttttaggcat aaggatagtt ttgttatccg acaattatta agtatcacgt tcccatttat
    78361 ttaatctaat aacatataag tatttaatat tgaatgaatt atctgcatgg ttatttttaa
    78421 aattgttgaa acagaatata gaggctatgt gctagggtgt tgaaaaggtt aggcatactc
    78481 gcataaagct ttagtcagta attcatatag atagtatttc gtgtagggca gtaatagatc
    78541 gcaacggtta taacctttgt ccgggtttat tatctctcaa aattccatta aaagttatgg
    78601 tagagcacca atcattaacc tgaaattttt atccatcatg gttatattta tgacaaaaat
    78661 cagttttttg gaacctaaat agtttttata tttaggcaag tcaaatgtta ctgtgtgaat
    78721 gaaatttagt gttattattt taacaatgtt atcgttataa caataaaaac tattaaattt
    78781 ttggattatg acggaaaccg ttgagttagt taaaaagctt gctgacaagc atgggcgatc
    78841 aagggagagc ttaataccca tattacaggg tgtggtgcag catgaacggt acatttctag
    78901 cgaagcgatg accgaaattg ctagggaact ggacatttcg gctgctaaag tttatggaac
    78961 agccaccttc tacagctttc tcgataccca gcctcgggga aagtatgtaa ttagggtttg
    79021 caagacaatt tcatgcgcca tgaagggtaa aaacccaatt ttacaggagt tggaggatat
    79081 tttgaagata aaggtaggtg aaactacgca aaaccgtaag ttttcgctgc ttgaaaccaa
    79141 ctgcctgggc tggtgccata agggcccggc catgcttgtt aacgaggagg cttacaccga
    79201 gctaacgccc gagaaagtta gagaaatcat taatgattac atgaaaaagt agaggagact
    79261 ataatcatgg ccagtaattt tttgcgacgt gttgacctga tatttgatca ggattgcaac
    79321 tttaaggagg tgttgaaatc ggcataccag cattccaacg acgagattat caaccagcta
    79381 cttgaatcag ggcttaaagg aaggggaggt gccggtttcc caactgccct taaatggaag
    79441 tttaccagcg agcaggctga tccggaaaag tacgtggttt gcaatgccga cgagggcgaa
    79501 cctggaacct ttaaggaccg tgagattctc tttaaggttc cccttaaggt gtttggcgga
    79561 atggctatct gtgccaaggt tatcaacgcc aaggaaggat ttatctacct tagaggagag
    79621 tatcgctttt tactttcgga gctggaaaat cagctaaagc tgttcaacga gattttgaaa
    79681 gatttgaaaa ttgattttaa aataaaaatt atagtgggta gtggcgccta tatatgcggt
    79741 gaggaaagcg ctcttttcga atcgatggaa ggcgacaggg gcgaacctcg caacaaacct
    79801 ccatacccta ccgtatcggg attccggaaa aaacctaccg ttatcaacaa cgttgaaacg
    79861 ctggtttatg cctttatgat tttccgctat ggcgcccgca aattcaagga actgggaact
    79921 gcagattcac gcgggtctaa ggtgttttct gtatctggag atactccaaa acctggaatt
    79981 tatgagttgg agctgggtat gaccgtcgag caatttgttg acgagtttgg agatggtgat
    80041 accaaagcgg ttcaggtggg tggcgcttca gggttttgcg ttccccgaaa aaagttcaag
    80101 gataccataa tcggatttga gggtgttcct actggaggtt ccatgatgct ctttaacagc
    80161 tcgcgatcca tgtacaacgt gcttaaaaat tacctggatt tcttttccga ggagtcatgt
    80221 ggacagtgca ctccgtgtcg cgttggatgc cagcagctta tacttggtat tgaagcggtt
    80281 aagaagggcg atcgccatgc tgcatacctc gatcagctgc agaaactcgc ccaaacaatg
    80341 aagataaccg caaaatgcgg tttggggcaa agcgtggcaa attcgttctt ctctattgtg
    80401 gagaacttta aggaagagat gatttactaa attaaaattt caaattcatg gctaaacaaa
    80461 taaacctcac catagatggt ttcccaatca ctgttgaaga gggaactaca attctacagg
    80521 ctgctaaaaa actaaatatc cacattccaa cgctatgtta tcatgacgat ttatgcgtgg
    80581 caggaaattg tcgtgtttgc gtggttgaaa tgaagggatc gaaaacactt cctgcttcat
    80641 gcgccatgcc tgctgcggag ggaatggaaa tttttacaaa tacaattaag gttcgcaccg
    80701 cccgtaagca catcattgac ctcttgctct ccgagcacaa cgccaagtgc accacatgct
    80761 acaagaacgg taattgcgag ctgcaaaacc tggcctcgga atacaaaatt tccgagccca
    80821 tgtttatcga tctggttcca tacaaaaatt accagatgga cgcctattca ccatcaataa
    80881 ttaaggatga cagcaagtgc atccgttgtc agcgttgcgt gcgtacctgc gaagaactcc
    80941 agcatgttag cgcactgggg gttgcatata aaggtgccaa catgaaaatt tccacctttt
    81001 tcgaaaatcc catgttcgag gtggtttgta ccaactgcgg ccaatgcgta aaccgctgtc
    81061 caaccggagc gctggttgaa cgcaactacg tggaggaggt ttgggatgcc atttataacc
    81121 ctaacaagca tgttgttgtt caaactgcac cggctgtaag ggttgccctt ggagaggcgc
    81181 tggggatgaa acctggtcaa atcgttaccg gcaaaatggt aaccgccctt agaaggctag
    81241 ggtttgattc agttttggat actgattttt cagccgacct aaccattatg gaggagggat
    81301 tcgagttgct tggcaggctc aaaaaggcat tggttgacaa ggatagtact gtgaagattc
    81361 ccatggcaac gagctgctct cccggttgga ttaagtttat tgaacatacc tatccggagt
    81421 atcttgacaa cctttccacc tgtaaatcac cccaacaaat gtttggggct ctggccaaaa
    81481 cctactacgc taaaaaacgt aacattgatc cggccagtat agtttcggtt tccattatgc
    81541 cctgcacggc caagaaattc gaggctgacc gaccggaaat gcactccagc ggttataagg
    81601 atgtggacta tgtgctcacc acccgtgagc tggctattat gatcaagcag gcaggtatag
    81661 agtttgacag cctaccggaa acaaactacg atagcattat gggagattcc acaggtgctg
    81721 ctgttatttt tggtgctacc ggaggggtta tggaggctgc cctacgaacc gcctacgagg
    81781 tagttaccgg tagagaggtt ccatttgatg gcctcaacat tacacctgtt aggggaatgg
    81841 agggtgttcg tgaggctgcc attaaaattg agaataccaa acccgaatgg aagttccttg
    81901 atggcgtggt tcttaaaacc gttgtagctc acggattgac caatgccaaa aaggtgatgg
    81961 atgtggttaa gtcgggaaaa gccgactggc acttcattga gattatggct tgccctgggg
    82021 ggtgcatcgg aggtggaggt cagcctattc ccaccaatga agaaatacgt aaaaagcgtg
    82081 ccgaagcaat ttaccgcgag gatgctaata agaaaatcag aaagtcgcat gaaaatcctg
    82141 aggtgattgc tatttataag gaatttcttg atgagcccaa cagccataag gctcatgaac
    82201 tactgcatac ccactacaag gaaaggcaga ggtactaaaa actagtgagt acctggctgt
    82261 atgcttaaag gctgtctctt aaaaggacag cctctttttt ttcacagtac gagtacaacc
    82321 ttctttattg catttagctt atctctgata aattcttagc tttctgattg taaatatttt
    82381 ggcatacaat tataggcgac ggggctgagc ggatggatcg ccagcacaaa ggctttgcag
    82441 aactcgcaat tagcaaccgg ttgttcatac gcccttgtac aatttgccca gcatgagcaa
    82501 caaaagtagg agaatgagta aaaatggatt tttcatcagc cgttagtcaa tgggtataat
    82561 cgttagccaa atagcgtatg tattatacat gtttaataat acatgttata ttaataacaa
    82621 taaatatatt tgtggcgtat aattattgta gtaaaaaata ttttatgcgg ataggtgagc
    82681 tggtaaagca tatagatgga aaggtgctat gctgtgagga gtgcctcgac agcgaggtag
    82741 tttatgcttt tgcctcggat ctgatgagcg atgtgctaac tgttaaatgt gagaattttt
    82801 tgctgattac agggttgtcc aacattcaag ctgtacgcac cgccgagatg tccgatattc
    82861 aagttatcgt atttgttcgg aacaagcggg tgagcgagga tatgattagg ttggcaaggg
    82921 aaaatagcat agttttggtg gagtgcagct gcaccatgtt taaggtttct ggaattttat
    82981 acggcttggg catcaaagca gtttactgat aacgatgaaa tttgaatata aagtacaggg
    83041 tggtgatttt gtgagagccg gccacgcatc aagtgaggtt aaaaaaattt taaaacagct
    83101 gaatgtagaa ccttcactgg tgaaacgggt ggtggtggca ctttatgagg cagaggttaa
    83161 tattgtagct catgcgtata acggaatggc atatgttgat attgatgcaa gtagggtgct
    83221 ggttaggctt gatgataatg gcccaggaat ttcggatatt gagctggcca tgcagcccgg
    83281 tttttcaacc gcatcttcaa ccgtaaggga gatgggcttt ggagcaggaa tggggttgcc
    83341 aaatattaaa agtaattccg atgaatttag catttccagc gaggtgggga aaggaactca
    83401 tttggaattt gtagtttacc taaacagtaa ataacgtgag aggaggttag agttggaaac
    83461 ttctacattt caccacgctt taaaaattca atccgatgtc tgcattggct gtgcacattg
    83521 tatgagggta tgcccaaccg aggctcttag agtaagagga ggaaaagcct tacttcaaag
    83581 caacaggtgt attgattgtg gtgaatgcta cagggtttgt ccaaccggag caattattgt
    83641 agaacaggat gactttaata gcattttcaa ctacaagtat agggtggctc ttgtaccatc
    83701 agtgcttctt gcccagtatc ccgaagatgt ttcggttgat atggccattc acgctctact
    83761 aaggatagga tttactaagg tagtaattgt tgaagactcc gtcgattacc taattaccag
    83821 gataaacgct tatattataa acaaccctag ccgaaagccc ataatctctt ctttttgccc
    83881 ggcaattgtc aggttggttc aggtccgatt tccctggctt gtaggaaaca ttatgttggt
    83941 aaagccccca atagatctta cttcatcatt cataagaagg aagcttacgg aggaaggaat
    84001 tcctgaacag gaggttggaa ttttttacat tactccatgt gcggccaaaa ttgccgcagt
    84061 taaaagcccc gaaggggagg atgtatccga aataaatggg gttattaaca tggatttaat
    84121 ttacaaccga acactaaggg atattcgatc tgggggtact aagcgaacgg ttgaagatgg
    84181 tgccgttaac atttctccga aaagcatcac ttgggcactg acaggtggtg aagctcttag
    84241 catagaagct aaaggccttg ccattgatgg catttccaat gttattgagt ttctagagaa
    84301 gatggaaacc agcgaggttc acggggttga tttcttggag cttagggcat gcgatgaaag
    84361 ttgtgccgga ggaatactta ctgttggaaa cagcttcctc tctgccgaac ggcttcggaa
    84421 gcaggccagg tatatcccag aatctaaaat aggttttcat actcaaataa aaatttcaga
    84481 gtacagtagt gtgctgaacg gcaaggttgg cttaaaccga ctggagccac gctccattgt
    84541 caagctagat gacagcatgg ttaaagccat gaacaagatg gaaagggtca accggtatct
    84601 ttgctacctg cctggcattg actgtggagt atgcggtaca ccatcgtgcc aggctctggc
    84661 tgaagatatt gttcagggag aaggctcgct ctcacaatgc gtatttttac agaaaatcat
    84721 ggaacagaac aagctggacc ctaagcattc cttccgtata atgaaaaaaa tttggggcga
    84781 aaacagattt attaaggatt gcgataaacc taaacataaa aaataattgg ttatgaccgt
    84841 actagatatt gtaaagtctc ttggcttaaa ggtttataca gaaacggtgg gtatggacag
    84901 ggagattacc ggtggctaca cctccgatct tctaagcgat gtaatgggca atgcacaagc
    84961 tggtaccgtt tggattaccc ttcaaacgca cataaatatt gtggctgtag cttcacttaa
    85021 ggatattgca gcaattttac tggtaaaagg acttgagcca gaacccaaca cacttcagaa
    85081 aagccttgag gagggaatac ccattctggg gacctccaaa caagcgtttg aaatttccgg
    85141 aatgctctac cagctgcttt acagtaaccg atgaaccttt accaggccga tttacatatt
    85201 cacagcgttt tatccccgtg tgccgatctt gaaatgagcc ctggcaatat agtttccatg
    85261 gcaaagaata tggggcttag cattattgca attactgatc acaactccac gcttcatgcg
    85321 ccattaatgc gtcgtttggc cgaaaatgca ggaatactcg ctatttacgg tgccgaggta
    85381 accacccgtg aggaggttca ttgcgtttgc ctttttgccg acgaggatag cagagctgat
    85441 tttcaaaatt ttctggaagt acatatccag aaggtaccta ataaacctga tattctagga
    85501 taccaggtag ttgttaacga gaaggaggaa attattgagg aagttgagta ctcactccat
    85561 ggggcgttaa atgttggcat caacgaactt gaggctgttg tgcatgcaat gaatggaatt
    85621 tttataccgg ctcatattga caggccgaag tatagcttga ccagccagct gggttttgta
    85681 ccccccgacc taaactatga tgcgctggag gtatccataa attcttcaat tgaaagtatc
    85741 tgcatacaca acccatcgct taaacgaaaa agatttataa ggagttccga tgctcacttc
    85801 atagaacaag ttgggtctgt aaccactacc ttcaggatgg aaaacctgaa cttcgatggc
    85861 ttgttgaagg caattaaagg gattgacggc tacgatatta tgctcccctg agggagctag
    85921 cctgatgagg ctgcctatac tttagatgat tttgatatga gggatttatc gttgcacctg
    85981 ctcgacatag ctcaaaattc aattgctgcc caggcaacca aaattgatgt tcatcttgaa
    86041 attaacccaa aacttgatta ccttgcactt atcatatccg ataatggaaa aggtatgaat
    86101 gccgagtttc ttcaacagat aacgagtcct tttacaacaa cccgtacaac ccgcaaggtt
    86161 ggattaggat tacccctttt aatgcaaaat gctgagcgca cgggtggtta cctggaaatt
    86221 acctcagctg ttggtaaggg aacaacgctt agagctgttt ttcatcctaa ccacatagat
    86281 tgccctccta tgggcgatat agcgggtgtt gctgctcagc ttatttgttc gttccctaac
    86341 atttcgttta gcttcagttt taaaacagat acttcagagt ttagcattac cacacttgaa
    86401 atagctcaag cactggaggg cattcctatt aatgatacca gtgtttttca gctagttaag
    86461 caggtgatag aatcctccct ggaggagctc aaatttcccg tttaggttat taaacatata
    86521 atttgttgtt agtttgttaa catttaaaat gtaattttgt caccgaaaag ttcaaaacga
    86581 tatataccca ataaaaatga atgtttaagg agtattgcta tgacaaaagt aaaatcatta
    86641 gctgatctga aaatgatgaa ggactccctg aagtcgaaaa tctcactacg ggaaaagggg
    86701 gaaaattctg aaaatctcgt tcagattaag gtggcaatgg ccacatgtgg cattgcttct
    86761 ggagcaaaag tggttatgga ctttttgctc gaggagctgg ataagcgggg gataaactcc
    86821 gtagtgaccc aaactggatg catgggttac tgctatgctg aacccaccat agaggttaag
    86881 cgtcccggcc aagaacccgt ggtttttggg tacgttgata ctaaaaaggc tgatgaaata
    86941 attgaaaagt acatcaaaaa tggcgagttg gtggatggca tcatccccac caactacgaa
    87001 accattgatg gtgaaaaata accctcttaa aagacagaag taatggcaaa gtataaaatg
    87061 cacctgctgg tttgtggtgg aacgggctgc agggcttcgt cgagcgattt gattgttgac
    87121 aggcttaaag ctgagattgc ttccaacggc ctggagaatg atgttcaggt tataatgact
    87181 ggttgcttcg ggttttgtga gaaaggccct attgtcaagg tgcttcctga taacaccttt
    87241 tacacccagg ttaaacctga agatgccgcc gaaattgtta gggagcacgc cctgaaggga
    87301 aggaaggtta cccgcctgct ctacaccgat ccggtaaaaa aggaacatgt atccgactca
    87361 aaacacatgg gtttctaccg caagcaaatt agaattgccc taagaaactg tggttttatt
    87421 aatcccgaaa acattgacga gtacattgcc cgtgatggct accaggcgct tggaaaagtg
    87481 cttaccgaaa tggacccgca gaaaacaatt gatatcatta agaaatcggg attaagggga
    87541 cgtggtggtg gcggctttcc taccggttta aagtgggaaa ttgccagcaa gaacaaagcc
    87601 gatcagaagt acgtggtttg caacgccgac gagggcgacc caggtgcttt tatggatcgt
    87661 tctgtcctag agggcgaccc acactcagta ctagaagcaa tggcaatttg cggttatagt
    87721 attggtgctt caaaagggct catttacatt cgggcggagt acccattggc tattaaacgg
    87781 ctaaaggtcg ccattgatca ggctcatgag tacggcttgc tcggcaacga catcatggga
    87841 acaggcttta attttgatat tgagcttcgc tatggtgccg gtgcttttgt ttgcggtgag
    87901 gaaactgctc ttattcattc catggagggt cttaggggcg agccaaccaa taaaccacca
    87961 ttccccgctg aaagcggata ccttgggaag cctaccaacg ttaacaacgt ggaaacgttt
    88021 gccaacgtag ctccaatcat tttgaaggga gccgaatggt acacctcgat tggaactgaa
    88081 aaatcaaaag gaacaaaggt ttttgcgcta gcagggaaaa ttaacaatgt agggttaatt
    88141 gaggtgccca tgggaactac cctacgagaa attatatttg aaattggagg gggcataagg
    88201 aacggtaaaa agtttaaggc agttcaaaca ggcggtcctt cgggcggatg ccttacggaa
    88261 aagcacctgg atattcccat agactttgac aacctggttg cagctggctc aatgatgggc
    88321 tcgggcggga tgattgttat ggatgaggac gactgcatgg tttctgtggc caagttttac
    88381 cttgacttta cagttgatga gagctgcgga aaatgcactc catgtcgtgt aggaaacaaa
    88441 cgactttcgg agttgcttgg taaaattact gatggcaacg gggagcttga agatcttgag
    88501 cgtttgcgca acctttcaaa tgttatcaag gatactgcgc tttgcggctt aggtcaaact
    88561 tcacccaacc cggtactttc aacccttgat aatttttacg acgagtacct ttcacacgta
    88621 gttgataaga aatgcaaagc cgggcggtgc aaatcgctta tgcagtatgt tattgatcct
    88681 gaggcatgtg ttggttgcac ggcctgcgca cgcaactgtc ccgttaatgc cataagcggt
    88741 gagagaaaaa aaccacacga gattaacact gccatctgca ttaaatgtgg agcatgtatt
    88801 gaaaaatgta aattcgatgc tattagcatt aaataacagg ctgaacaatg gaaacagtaa
    88861 aacttacaat agatagcaag caggttgaag tacctaaagg tactacgata tacaaagccg
    88921 caaaaagcat gggaatcgat atcccggtgc tttgctacat ggatcttcat gacctgggtg
    88981 ttgagaataa acctgcaggt tgtagaattt gcgtagttga aattgctggt cgtcgtaatc
    89041 tggctcccag ctgttcaacc gtttgcatgg agggcatgga ggttaagacc catacaacgc
    89101 gggtgataaa tgcccgccgg acggttatgg agctgctact ttccgatcat ccaaaggatt
    89161 gccttacatg tcctaaaagt ggtaggtgtg accttcagga tatgtctgtt aggttgggta
    89221 ttcgtgaaat acccggacag gaatttgccg ccatgtcaac ctacaaaaaa gatttttcgc
    89281 cctcaattat ccgcgatgtt gataagtgca tcatgtgccg taggtgcgag accatgtgca
    89341 accaggtgca aacagttggt gcactaagcg ccgtgaatag gggttttatg gctgttgtgg
    89401 cccctgcttt tgagcaggat ctggtaaaat cgccctgtac atattgcggc caatgcgttg
    89461 ctgtgtgccc tactggcgct ttaaccgagg ttgaccacac tgccaaggta ataagggcgc
    89521 ttgccgatcc caccaaaacc gttatggttc aaacagcccc tgcagttaga gctgctcttg
    89581 gcgaggagtt tggcctggag ccaggaactc ttgttacggg aaaaatggtg tcggcacttc
    89641 gaaaaattgg gttcaactat atttttgata ctgattttgc ggccgatttg accataatgg
    89701 aggagggaac cgagcttctc gacaggttaa cccgccacct caatggcgat aagagcgtta
    89761 agcttcctat tcttaccagc tgctgccccg catgggtaaa cttctttgag cataactaca
    89821 ccgatatgct cgatgttccc tcaagcgcaa aatcgcccca gcaaatgttt ggagctatag
    89881 ccaaatcgta ctttgctgaa aaacttggca agaaacgtga ggatttggtg gttgtatcgg
    89941 tgatgccctg cctggcaaag aagtatgaat gccagcgtga tgagtttaag catgagggca
    90001 accctgacgt tgacctatcg ctgtcaaccc gcgagctggc caaccttatc aagcagttca
    90061 acatcaactt tatggagctg cccgatgagg atttcgatcg accactgggc gaatcaacgg
    90121 gagccgcagt gatatttggc gcaaccggtg gcgtaattga ggctgctgtt cgtaccgctt
    90181 atgagctgca taccaagaaa gcacttccca gggttgactt taccgagctt cgcggtatgg
    90241 atggcataag gactgcaaca attgattttg atgggcttcc gataaagatt ggaattgcgc
    90301 atgggctggg taacgcccgc aagctgcttg accaagttcg ttcaggacag gctgatttcc
    90361 atgccattga aattatggca tgccctggag gctgcatagg tggaggtgga caacctctac
    90421 accatggcga ctcatcaatt atcaaggctc gtatggccgc aatttacagg gaggatgccg
    90481 gtaaatcaat ccgaaaatcg catgaaaatc cctacatcat acagctttac gaggagtttt
    90541 tgggtaaacc tatgagtgag aaatcacacc atctacttca tacccaatac tttgataaag
    90601 gtaaaaagga tatttatatt aaatagggag gaagaaaccc atgtcgcaca ttaaagtaga
    90661 acttaagccc gatcaggtgg ctaaaataaa ggaaatctgc aaatcctttg ggaataatcc
    90721 cggtgagctt atcaacgtac tccatcaaac tcagggcaca tttggctacc ttccggcgga
    90781 agttcaggaa gttgttgctg cagagctaaa tattccggtt gccaaggttt acggagtagt
    90841 tacattctac tcgtttttca ccatgattcc caagggtaag cacccgatta gtgtttgtac
    90901 cggcacagcc tgctatgttc gtggagccga aaaggttctg gacgaaatga agcgcatact
    90961 aaacgtcaaa gtgggcgaaa caactcctga tggaaaattt tcgttgaact gcctgcgttg
    91021 cgttggggca tgcgggctag ctcctgttgt ccttataggt gaaaaggttt acggaagagt
    91081 ttcccccgat ggggtaaagg acattcttaa ggagtacgaa gactagttca aatttcaatt
    91141 atctttcata gctttaggtt taggttggtt gaaaagaagg ggaaagccat ttaaattggc
    91201 actccttctt tttttgttac ttcacatatt caataaaaat tcccagggaa acaccaaaat
    91261 tcaggttgat gctttccgca taatatgcct aattgtatct tcggaaatgc tgggtgtgag
    91321 tttttccatt gcagccaaaa gactttttag ggcaaaagac tcctgtattg gcggaaggga
    91381 atgctttact gatttctttg catgttttga taagccagag gtattgttgg ctaattgggt
    91441 aaagctattt gtcataggca ggaaacaatt ttatctaata acggcaacca gccactatta
    91501 ttacacgaag tagaggataa attgagaccc ctatttcccc ttgatggaag agaaggagga
    91561 agcaaggata aagggtaaaa aaattcaaaa ttcaaaattc aagaacttgt ccgcctgctg
    91621 gcggaacttg tccggctcct gacggattcc
//
LOCUS       DAOSHM010000003        75423 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_271_length_75423_cov_20.803697, whole genome shotgun sequence.
ACCESSION   DAOSHM010000003 DAOSHM010000000
VERSION     DAOSHM010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 75423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 75423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..75423
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_271_length_75423_cov_20.803697"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3274)
                     /gene="ygfK"
                     /locus_tag="PK839_00875"
     CDS             complement(<1..3274)
                     /gene="ygfK"
                     /locus_tag="PK839_00875"
                     /inference="COORDINATES: protein motif:HMM:TIGR03315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HOG71316.1"
                     /translation="MSDKFFPIPIEQLLGIILHELDSNQSILGIPSKLFFNPQKSNRL
                     ATEIFGQKIATPLGVAAGPHSQMAQNIIAAWLMGCRYIELKTIQTLDELEVSKPCIDM
                     QDEGYNCEWSQELKVEQSFNEYLNAWVAIHILNHKLGLGSSPQTIFNMSVGYNLEGIK
                     KPNVQWFLDRMTSCETELKTKINLVRPIYPAIDSILIPPQLSNSITLSTMHGCPANEI
                     EGIARYLLTERKLHTLVKLNPTLLGRQELRRILNDELKFKTSVPDAAFEHDLKYPDAV
                     QIIRSLQGDAERMGLQFGLKLTNTLESVNNRGTFKSEVEMMYMSGRALHPISINLASK
                     LQNEFDGQLLISFSAGVDAFNVVDTLKCGFRTVTVSSDLLKPGGYMRLNQYIEAISAE
                     LLKNKLDSVGGLAHLKNGAAPTDYLNSYAGRVIENKRYQRSYLHEPTIKTTRELDYFD
                     CISAPCRDTCAANQDIPDYLHHAANSQFDKALEVILQTNPFPSVTGMVCDHLCQNKCT
                     RINYDNPLLIREVKRFVAENASIKLSQKPLNGKQVAIVGAGPSGLTCAYYLAQAGFKV
                     DVYESESKAGGMVRYAIPGFRLTSEALERDIARILQLGVTIAYNHRITSEEFSKLKST
                     YDFLYISAGAQKATPLNIDGIEAAGVYDSLAFLKDVKAGNIKGVGKNVVVIGGGNTAM
                     DAARTAHRLVGTNGKVTVVYRRTIDEMPADEGEIKAVMAEGAEIVELSAPERLIIEKG
                     NVCGLVCSRMKLEGTDAKGRPKPVRVNNSEFTIPCDTVIPAIGQEVDIDFVPKELLRA
                     HTTTYKTLLHNVYIGGDALRGAATAIKAIGDGRKVAEQIINQAGIQRSIKTPTGRSHS
                     KEELIMKRAKRSYAPELPELSPSESRTFSLVSQTLDEQSIVSESGRCLQCDEICNICT
                     TVCPNLANFSYDISPVKYNMQRVLVGEDGQLKFEPDGVFEVKQQYQILNIANFCNECG
                     NCATFCPTSSAPYLNKPKLHLTTKSFNSSDEGYMLSKLEDRNVLIYKNEGGIKTLSET
                     AGELIYETDHVAARFDHDTFELKEVNVKTPCVRQIQFQHAAEMSIILKGAKARIKDNT
                     RIK"
     gene            3368..4042
                     /locus_tag="PK839_00880"
     CDS             3368..4042
                     /locus_tag="PK839_00880"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HOG71317.1"
                     /translation="MTIKVKKNTSAIILAAGKSERMGYPKFMLRCADGQTFLQKLVSG
                     FLLFGCRQVVVVLNEHEYQKVLLQIPEFPSEVLLVENSHPEWGRFYSLKLAVDRIKPI
                     DYIYVGNIDNPFVDAELLGLLYHSAAQADYLMPEYKGRGGHPILISQKIAEDIKTRTT
                     YDYHLKEFLSSYSSLRVEVNNPLVLANINTIDDYRQYFGDWKLSKYQAPIQNSYDGFD
                     VKKLTE"
     gene            complement(4627..5520)
                     /locus_tag="PK839_00885"
     CDS             complement(4627..5520)
                     /locus_tag="PK839_00885"
                     /inference="COORDINATES: protein motif:HMM:NF039989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOG71318.1"
                     /translation="MKPNLTAQSTNKLIDEKDITKLSRFFKGKAGEKFLKFLMHLLAI
                     DKVNWVYDHSFDYSGPEFTSRLLSDIGVDYKVGNPERLEKLPKGAFITIANHPYGGLD
                     GIMLIDILARIRPDFKIMANKQLSLIKTLASNFISVLPKTDANKGYSPFNFQSIRDTL
                     NLIRSGHAVGFFPSGAISDFKFRNLRVSDREWQDSIIRLIQKAQVPILPIRFFDRNSI
                     FFYFLGLIDWRIRSLRLPHEIFNKQGQCQRIGVGEIITVGEQAKYPDSEMFGMFLRNM
                     VYGMPLPSAFIQKSALCLPTK"
     gene            complement(5673..7946)
                     /locus_tag="PK839_00890"
     CDS             complement(5673..7946)
                     /locus_tag="PK839_00890"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOG71319.1"
                     /translation="MNNFNVNPMNNIDSINHVTGKSIYLDDITTLKGTLHLEIFGSAV
                     AHAKILSIDLTYAARVEGVVGIFTAKDIPGENQIGGIVEDEVLLAETEVHYQGQPIAI
                     VAATTEIAARKAISLIRVDYEELPAVTDPRVAQKNGMLIIPPRTFSMGNTDEAWCKCT
                     HIFDGRVEIGGQEHLYIETQGAYAYPTEGGFLRVHSSTQGPTAVQRTIARVLGLPMHQ
                     IEVDVQRIGGGFGGKEDQPSQFAALAALAAHLLKQPVKLSLSRHDDMRMTGKRHPYSA
                     DFKIGLDKNLKIIAYETTLYQNGGASADLSPAIMERTLFHSTASYFIPNTRVTVYSCR
                     TNLPPNTAFRGFGAPQGMFVMESAIAKAAHELGVAAQTIQELNLISEKDEFQYGQVAE
                     NAYATKCFERAKEQFKIEVLQKEIKLYNSKNRFSKRGMAVMPLCFGISFTNITMNQAR
                     ALVHIYQDGSVSVSTGAVEMGQGVNTKLAQVAAITLGIGIERVKIYSTNTSRVANTSP
                     TAASTGADINGNALLIAATRLLERLKQVAAEMLSCRASEVEIKNEEVLCRGERSSTSW
                     QKLIQTAYLKRICLTENGHYSTPKIYFDKTKEKGHPFAYMVYGTGIVTTTVDCIRGTY
                     SIDEVLVVHDFGNSLNPTIDTGQLEGAIVQGIGWATIEELMFNNKGTLLSNSLSTYKV
                     PDIHFAPKSIRCIPLEADGPEQAIMKSKAIGEPPFMYGIAAYFAIQNAIMEFNPSYQP
                     SYDLPLTPEKVLMGLYS"
     gene            complement(7968..9368)
                     /locus_tag="PK839_00895"
     CDS             complement(7968..9368)
                     /locus_tag="PK839_00895"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HOG71320.1"
                     /translation="MVTFLLNNRLVETYKNPGMSLLDYIRYEADLPSTKVGCREGDCG
                     ACTVMLGELTGEHVEYKSIVSCLTPLVNVHGKHIVTVEGINLDKELTPVQKVMVDNAA
                     TQCGFCTPGFVMSFTSYCLSHERPSIEGAINAVSGNICRCTGYKSIERAAADLNQIIR
                     DKNLVNPVGWMVEHGYLPAYFLDVPRLLGGIHPLDYTAPENSTYIGGATDLMVQKPNH
                     ILNDPIMPVGALRGLKGIELDGSRCVIGAATTFNELTHSDLLCSIIPTLRNIMPFVAS
                     DQVRNSATIAGNIVNASPIADLSIILLSLNAEISTSLNGNMRIQPLREFYTGYKKLSL
                     YEGERVESISFEIPSPKSIFNFEKISKRKYLDIASVNSSILACTEGNVITSCHVSAGG
                     VAPIPTYLFNTSMFMVGKTINQETFEKANTIMQAEISPISDIRGSADYKRLLLRQLLF
                     AHLSKLFPELLKVTWL"
     gene            complement(9598..10998)
                     /gene="mnmE"
                     /locus_tag="PK839_00900"
     CDS             complement(9598..10998)
                     /gene="mnmE"
                     /locus_tag="PK839_00900"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004320322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HOG71321.1"
                     /translation="MLDDSTIVAIATPPGMGAIAVIRVSGVDSITLTDGVFKPVMAGK
                     SLLKAKPYTLHYGHIVDMERVIDEVLVSIFHAPHSYTGENAVEISCHGSVVIQQQILE
                     LLIRRGARLAKPGEFTLRAFLNGKMDLSQAEAVADLIASTSEAERRVALQQMRGGFSD
                     KLKLLRDQLLRFTALIELELDFSEEDVEFADRKQLEALVGNIHEEVSRLADSFSMGNA
                     IKQGIPVTIAGKPNVGKSTLLNRLLNEEKAIVSEIAGTTRDAIEDTIQLAGMNFRFID
                     TAGLRHTTDEVESLGIARTHSKIEQAKVVLYLIDAAESMSVGLTEVEKFQQKLTNQQT
                     LMVLLNKADRAEAQHTELLAQNIESHLHCITLIISAKSGQNINELTQNLVNIGMSAQP
                     DGNNVVVTNARHYESLVNARDSLARVLSGLKSNLTGDLLAVDIHQALYHLGSITGEIT
                     NDEVLGYIFSKFCIGK"
     gene            11652..13733
                     /locus_tag="PK839_00905"
     CDS             11652..13733
                     /locus_tag="PK839_00905"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010992235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HOG71322.1"
                     /translation="MIVILVAASCKNNSSEVKNPLLAEFDTPFQTPPFDKIRHEHYAP
                     AIDSAIAEAKAEVEAITSNPAKSTFENTIEALDRSGRRLNLVSNILFNLNESETDSTL
                     QGIARDAAIKLTEFSNDVNLNPELFKRVKQVWEMRDSLKLTTEQQTLLSNTYKGFIRN
                     GANLSDSDKEKFRAISKELADLSLRFNENLLAETNSYQLHITNESDLAGLPASLKDAA
                     AYTAQQKGKGGWIITLDYPMYGPFMKYSENRELRKQLYMAYGSRCFKGNEYDNQAIAK
                     KIANLRLEIANLLGYPNYASFVLQDRMAENPERVNNFLKELVDASMPAARREVEEVRG
                     FAKELGFNENIQPWDWSFYSEKLKNSRYSYNEEELKPYFELNRVINGVFTLANKLYGL
                     TFTPNNQIPVYHPDVKAYEVYDATHKFMAVLYLDFFPREGKSGGAWMTSFRSQYRDGS
                     TDVRPFVSLVTNFTKPTATQPSLLTFYEVSTFMHEFGHALHGMLSECTYSSLSGTSVY
                     RDFVELPSQLMENWAVEKEFLDMFASHYQTGEKIPQELVQKIIDSRNFLAGYSSIRQL
                     SFGVLDMAWHTVEKPVTISVGEFEKRAIANLEVLPTVEGTNQSVGFGHIFDGGYAAGY
                     YGYKWAEVLDADAFEMFKEHGIFNPEVAASFRTNILSKGGSEHPMELYKRFRGHEPSI
                     DPLLRRSGLKN"
     regulatory      13831..14018
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            14191..16041
                     /locus_tag="PK839_00910"
     CDS             14191..16041
                     /locus_tag="PK839_00910"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOG71323.1"
                     /translation="MSLSDTIRMDEVKVYGNLRESHSIGATIYKMDSVKLKIFQNRSA
                     AELLAMGSVNLKSYGVGGLSSLTMRGGNSSQTIVVWNGVNIQNPMNGGANLSLFPVNM
                     FSSIRLQYGGSGTIYGSGAISGILILNSNALFSQPNGGELSLSYGSGNTRSLSSQVKY
                     GDKSRAISVKVFGQLADNDFEFRNIYKIGSPKERITHAESKQLGILTDVNMRLHEKLF
                     WSFSGWGQLGDKNLQPIMSSTQPSYANQVDRSLALVSNLSLSPSKRILFNLKNAASLG
                     TIDYSDKGVNIFTNNSYASILTELESKVDVLNNGEVSMGINHTLETASSDDYVGSAKR
                     NRVSGYASFRKPLFSSRFLSVLSMRGELVDGDFKPVIYSVGAEWLPTKKLIFKMNASK
                     NYRIPTLNDLYWASTTYTEGNPNLEPESGWGGDVGFQVKSISSDRAINVSNSLFYSNI
                     ERWIVWLSIPTVSDSKWKPFNMSKGESWGWESQVDWSLRVNSLTIRVEGFYTFTHSVF
                     SEQPGSRAQPMIYTPTHRFSGSLNLQYEKYGLGYYHGFTGKRYRDFQSQLPAFNLANL
                     FISYKFNLLWKVVTAGLSVNNLWNQDYQLIAGYAMPLRSYMFTINIDFTR"
     gene            16070..17143
                     /locus_tag="PK839_00915"
     CDS             16070..17143
                     /locus_tag="PK839_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG71324.1"
                     /translation="MKKLVKLMMAMLVLAFAFASCGGDDETQYAEFENGAIILNEGAY
                     GNSNASITFIGYGSDIADNQIFYRINGRELGDVLQHAAIYNGKIYMVLNNSGKVEVAS
                     SATFIQLQTITGLVNPRYVAAKDGKIYVTQWGSWGEQGTVEVFDANSFSHSNTIKVGT
                     GAEGLLCTDGKIWVANGGGYGLDSTVMVVDAATGAVSDTVTVGYNPKELVEDASGNIW
                     VICSGADIYDDSWEVVGHLPSKLVMIDRQSRQVVHSLIISNTSHPAHIDVSSGKETVY
                     FGGGFGVNGIFSVSITQPVIPQTPVIDGFFYGFNIRPSNGDIYCMVAPNFTSAGELKI
                     YSSAGAALKSYSVGIAPSTALFN"
     gene            complement(17216..17890)
                     /locus_tag="PK839_00920"
     CDS             complement(17216..17890)
                     /locus_tag="PK839_00920"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HOG71325.1"
                     /translation="MPMCFSINLNIEKSALEKSYHAKFSASYGYTPFYFASAFENPMV
                     PVIANRDSSLIVPMVWGLIPHWVTTPEQANLIRSKTYNARIETIHEKPSFRYAARSNR
                     CIIPVTGFFEWQEVNGKKVSWLIKPRADATFSIAGLWNSWTNVETGELIDTFTIITQP
                     ANELMSQIHNTKKRMPAIVDKDLVKSWLDAKLPTLEYDAILRPLDSELMDAHVVPSPL
                     RRPKSQ"
     gene            18286..19899
                     /locus_tag="PK839_00925"
     CDS             18286..19899
                     /locus_tag="PK839_00925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG71326.1"
                     /translation="MPVSSKGVLELTLDYDCDYIFSFEKEGYISKKIAIDTRIPDKLK
                     ESFDKVTAFDVELFKQSENEVKEYKNPVARIQFSSQISDFVFDTDYSLRVQQEIAQAN
                     KDLVEERKKQESLDLAKKEEEKRQQLLAEAQKRVEAEAQKKAAEEKARQVEAEQLKKE
                     AEERRLAEAKAKEEALKREEELRLKAREEARIKAEQEALRIAEQKRIEEEKLKQEQER
                     IRQEEEAKRKAAEEARIKEEQRIREEAERIRVAEEAKKKADEEARLKELEQQKKEAEE
                     RRLAEAKAKEEALKREEELRLKAQEEARIKAEQEALQIAEQKRIEEEKLKQEQERIRQ
                     EEEAKRKAAEEARIKEEQRIREEAERIRVAEEAKKKADEEARLKELEQQKKEAEERAI
                     ADAKLKEEQRRIELEKAQKEAERKKFEEEERLKAEQQQKLLFEEQKKRDKELRLKLVA
                     EKMSAKIGTEDAERVVTLLTPEDVTKKYTRERTDEVIRLPRIVITRTVYNRNDKITFY
                     TRVDHEYGAIFYFKDGYIISEEQYKAETK"
     gene            19961..22009
                     /locus_tag="PK839_00930"
     CDS             19961..22009
                     /locus_tag="PK839_00930"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOG71327.1"
                     /translation="MKEFVTACPRNCYSTCSFIVQVEKDKVVGIKPLSANKATPTGVC
                     LKGLSYVERANSPDRILYPYKKNGSGDFIRISWDEALSTIAGKLRYFKKTFGSQSVLF
                     YAASGMSGLINEFSGRFWREIYGGVTTTYGNLCWPAGLEATRLTLGENKHNAPWDLEN
                     ARLIVLWGKNPAESNIHEMIPLSTALDKGAKLAVVDPRRTSSSQRADLLVQPLPSTDA
                     ALALAVANILIKNSWIDEDFISSYVNGFEEFKAKAMSCTPEWASSICDISVESIEKLA
                     WLIGNVKPMTIIPGYGMQRYTNGGQTIRAILALSVITGNIGKPGACWHYANLQSYVFD
                     EVLEPICYYPPEEPDSTTRRSISTALLGEDMLSTTNPQLKMIWVERGNPLTQNPNTGS
                     VLEAFRKLDFRVVVEQFFTDTALEADIVLPAKNMFEQSDIIGSYWNPYIQLKQKVVEP
                     AGEVKPETEIYWLLAKQLGFNENTLKNILLPPGNDEVEKFLAQKLAKFPGLSLELLKM
                     GPVVPPGHQEIAFSDFRFATPSGKIELLSEQVASLWNANPLPEFLPAMEISDANSTYP
                     LLLLTPNTKNRIHSQFGNLKIIKFINGNPVVQMNPSDAMHRGIKNGSLVKVYNARGSI
                     QVSAQPTYEVRKGCVVIYNGWWKSQGAALNTLSAGRETDMGHGAAFHNNRVEVNLVKK
                     "
     gene            22013..23350
                     /locus_tag="PK839_00935"
     CDS             22013..23350
                     /locus_tag="PK839_00935"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF016838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HOG71328.1"
                     /translation="MQYNGFIFDYARCVGCHACVVACYNENGVQPPVLWRQVVGVNPE
                     KVPLYGFVSLSMACNHCTDAPCLKACPASAYYRDQETGAILHSNKRCIGCKYCTWACP
                     FDAPKYNPLRGVVEKCTMCNERLKSGEIPACTEACPTGALSFGSIETAASIQAPGISA
                     KPFSPRIVTKNEDIVNHIPHIDEQVTGYSPLQHTGMKHFAPSKINPIHELPLAIFTLI
                     CSLMVGWVGSMPWVGIASRGITLFILLGIVGALLSTFHLGKPFRALRSVANVKSSWLS
                     REILLFTLFFSLSALNLIFFNLIVLSVIAVILGLLLLVAVEMVYSVTDKKYKIHVHSA
                     NVVITALTIFSIFNSFERLLVLVLVLKAALYILRHANYSVSSLEFIFSFIRVFLGILI
                     PLGSIFFSNQHISMLMILFILIGELIDRIEFYGEQQVDTPRLLANNFWDKSSV"
     gene            23500..23889
                     /locus_tag="PK839_00940"
     CDS             23500..23889
                     /locus_tag="PK839_00940"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HOG71329.1"
                     /translation="MDKFYIKGTAETPTVSFDSTGNTFEISGKSLPEDVKEFYHPIIK
                     WLTEYCQSPNDATTLKVRMEYFNTASSKMILELFELLNKLHLSGRNAAIEWYYQEDDE
                     DMMDAGNDYADMLDLPFKIFGYQTLLK"
     gene            23905..24288
                     /locus_tag="PK839_00945"
     CDS             23905..24288
                     /locus_tag="PK839_00945"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HOG71330.1"
                     /translation="MVERFFNEPSAVTPLVEFDQQQGIFKIQGKSLPEDVKAFYMPLL
                     QWWDEYRNSPNDTTNLVLDFDYFNTASSKMILLLLSKLKDLSLLGKNVRVTWCFPSHD
                     SEMEEAGEELAEILNIPFDFIPKNS"
     gene            24306..24713
                     /locus_tag="PK839_00950"
     CDS             24306..24713
                     /locus_tag="PK839_00950"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HOG71331.1"
                     /translation="MEKLYIEPKKDSPEVIFDPKNGNFSITGISHPENVATFFEPIFS
                     WLEAYFVELGRTDSIRMKPINVRLFFKYINSATFKQLVTLMQKFKQFVETGYKVNITW
                     QFEPDDEDMKEAGYELIEISGINTTFSCEVCNN"
     gene            complement(24797..25891)
                     /locus_tag="PK839_00955"
     CDS             complement(24797..25891)
                     /locus_tag="PK839_00955"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HOG71332.1"
                     /translation="MNRVLVATGGGDCPGLNAVIRAVVKRASQEPDWEVIGSIQSFDG
                     ILREPTEIRVLDEKAVSGIHVQGGTIIGTTNKGGPFAWPIKNKDGSWGAVDRSDEMIR
                     KLQYLGIDAVISIGGDGSQKISQQLYEKGLNIIGVPKTIDNDLSATDFTFGFQTAVQI
                     ATDAVDKLVTTASSHNRVLILEVMGRFAGWIALHAAIAGGAEVCLIPEIPYDINKVAE
                     KLNSRYNKGKGHAIVVIAEGAKPIGGTLTSEQSDEVGYNNLRLGGVAHSLTNDLKRIG
                     FEADIRETVLGHLQRGGIPVAYDRVLATQFGVKAFEMVLQGKFGEMVAYRHPDIISVP
                     LIDAINRPNYVDPNCDLVRTARGVGISFGD"
     gene            26187..27566
                     /locus_tag="PK839_00960"
     CDS             26187..27566
                     /locus_tag="PK839_00960"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOG71333.1"
                     /translation="MRNSKNFTGHQLLMAILLAFILVFSSCKYSAKEEIPFQVQHADW
                     VYNATIYEVNVRQYTPEGTFKAFESHMPRLKELGVEILWFMPIHPIGEVNRKGALGSY
                     YSVKDYYGVNPEFGTLDDFKSVVAQAHQLGMKVIIDMVANHTSHDAVLVKEHPDWYVH
                     DSLGNLVSPFDWTDVAKLDYTKPELRKYMIDMFKYWIKDIDIDGFRCDVAAEVPVDFW
                     NEARAELNRIKPVFMLAEASNVELQKFAFDADYDWELHHTLNALAQGKKNVNDLEQYL
                     LKKYATYPRNTIKMNFITNHDENSWNGTEFERMGDAVKAMAAFTFVIDGMPLIYTGQE
                     VGFNRRLKFFDKDEVSWKDSLNLFPFYKKLVELKKSTPVLAAGDRGADIFRIKSNADS
                     AVFAFTRKNESQALFAIFNLSANQQIATLTGNDYAGTYTNVMTGEKFVFQGGEEISLK
                     PWEFYIYCN"
     gene            27819..29009
                     /locus_tag="PK839_00965"
     CDS             27819..29009
                     /locus_tag="PK839_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HOG71334.1"
                     /translation="MRYSVPVSNKIHWIGVNDRRKRLFENLWPLERGVSYNSFLIVDE
                     KTALVDTIDGTAVGNYIERIEQLLNGRPLDYLIVNHMEPDHSGEVKAIIDRFKGVKLV
                     GNVKTFKIVEAYWGITEGLIDVEDGRTLDLGYHKLKFLMTPWVHWPETMMTYDTTDQI
                     LFTGDAFGSFGAFEGGIFDDELDPLYFEDEARRYYSNIVGKYSNMVQKAIAKLEGIPV
                     RTIAPTHGPVWRSNPTIIMDWYNRWSRFEAAKGAVIIFASMYGNTEDIADYLARRLCE
                     NGVRNIRVFDVSKTHISYLISEIWKFNGLVLGSCAYNSQMFPLMEQLTHELLHIGLKS
                     KHLALFGSYSWNGGGVKNLAKFAGESELNLVSEPLEIYGKPTPQKLKPFDTLAETLAH
                     KILE"
     gene            29075..29464
                     /locus_tag="PK839_00970"
     CDS             29075..29464
                     /locus_tag="PK839_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016270902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HOG71335.1"
                     /translation="MEFTIPIGAECIVPEVVSSDNIASKYGSGMVEVFATPAMVALME
                     KAAMIAVRPFLPEGYSTVGVEVCVKHTKATPIGMEVWSKAVLKEMDGKRLTFEVKAWD
                     ANGEIGTGTHIRYIIDSRRFMEKIAAR"
     gene            29512..30279
                     /locus_tag="PK839_00975"
     CDS             29512..30279
                     /locus_tag="PK839_00975"
                     /inference="COORDINATES: protein motif:HMM:NF013782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HOG71336.1"
                     /translation="MDLNSYSLPQYLTRSMVVQSYEVDFNQRFSITSIFNHLQEIAWE
                     HADKLSYGYNHLREKGWFWALSRVEVEFYRFPVWQEAVTLVTWPRGADGIFALRDFEF
                     YDSNGNKLIAATSSWLVVGVENRRPVRIAEWYLESDFASRSALNRNAAKIPESIVQPA
                     FTEQFEVRAGDIDMNLHVNNVRYIDWAYNTFTISSLRKYFPKKISVNYNSEAKEGDSI
                     LINRKNIPGNSTLININSRLEGEKNYCRIQFDWRNLE"
     gene            30332..31870
                     /locus_tag="PK839_00980"
     CDS             30332..31870
                     /locus_tag="PK839_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008672909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4301 family protein"
                     /protein_id="HOG71337.1"
                     /translation="MLTKLDLKQLEERGITPQQVEGYMRMFREGFPFLPIERPATVGD
                     GIISLEDDEVDDLVEMFKDFQGSKVKFVPASGAATRMFKHLFEFRDEYPVKGLECFND
                     KGFNSAYSFFQNIKRFPFYDKLYNELWSVGYRLESLLEKKDYLPVVNALLDSNGLNYG
                     SLPKALIHFHRYGEIARTALEEHLVEAAKYARDQHGVANLHFTVSPEHLKGFQELVER
                     VKSFYENHYEVTLNVAYSVQKPTTDTLAVDMENNPFRNPDGSLHFRPGGHGALIENLN
                     ELNEDIIFIKNIDNVTPDHLKAATIRYKKALAGLLVSYKNLVNGYIERLLNEPVSEEQ
                     LALMLEFTTDELCILPPANLNRQNSDAVKKYLMEILDRPIRVCGMVKNQGEPGGGPFW
                     AKNPDGTTSLQIVEQSQVDMGNPRVKEIFGRSTHFNPVDIVCSIRNYKGEKFNLSNFL
                     DPQAGFISIKSKNGKQLKALELPGLWNGAMSRWITFFVEVPIATFTPVKTVNDLLRSE
                     HQPE"
     gene            complement(31970..32737)
                     /gene="truA"
                     /locus_tag="PK839_00985"
     CDS             complement(31970..32737)
                     /gene="truA"
                     /locus_tag="PK839_00985"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HOG71338.1"
                     /translation="MEQSAIYRYFVELKYRGTRYHGWQVQANANSVQGEVNRALSTLL
                     EAPTETFGAGRTDTGVHARFYVAHFDSTAAGLDADPTLLYHLNRILPADIAITRIIRV
                     RQDANARFDAIYRGYEYLICREKSPFLNGLAWMYYGHLNVEWMQLATQQLVGYSDFTS
                     FSKLGSNSKNNTCKIYHASWHQQNDMLIFSIKANRFLRNMVRAIVGTLVDVGRGKITP
                     EEFASIVEARDRSLAGTSAPAEGLYLTEVKYPKEMFL"
     gene            33059..34060
                     /locus_tag="PK839_00990"
     CDS             33059..34060
                     /locus_tag="PK839_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008149530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOG71339.1"
                     /translation="MSDTAAVNIKELNERIRLESSFVDIINIEMGKVIVGQKHLVESL
                     LVGLLADGHILLEGVPGLAKTLAITTLSNIIDVKFNRIQFTPDLLPADLIGTLIYSPR
                     SEQFNVKKGPIFANFILADEINRAPAKVQSALLEAMQERQVTIGDQTYNLEEPFLVLA
                     TQNPIEQEGTYPLPEAQVDRFMLKVNISYPKQEEERMIMRENLLQTFPKPERVLKPAD
                     IINARKVVREVYMDEKIEKYILDIVFATRFPADVNLQKFAPMISYGASPRATISLAIA
                     SKAYAFIKRRGYVIPEDVRAVCHDVLRHRIGLTYEAEAESITSEDIITEILNSVEVP"
     gene            34062..34481
                     /locus_tag="PK839_00995"
     CDS             34062..34481
                     /locus_tag="PK839_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG71340.1"
                     /translation="MSIFRILPLALLLVNLSIPMQGQNLIGLTKGEILKLVPTSCKGF
                     NFVKDIQNGDKSFIKFENPTDEQTLLFMLNSKEICTSVSRMYNTWLYDKVYKDLCCEY
                     KQVSMDTWIDSKNGNDYEVKLKRGEWFITVTIRPLIK"
     gene            34487..35374
                     /locus_tag="PK839_01000"
     CDS             34487..35374
                     /locus_tag="PK839_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012055625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOG71341.1"
                     /translation="MEASEILKRVRKIEIKTRGLSQHIFAGQYHSAFKGKGMAFSEVR
                     EYRYGDDIRSIDWNVTARYNQPYVKVFEEERELTVMLLVDVSGSQQFGTDFLLKRQAL
                     TEVAAVIAFSAILNNDKIGAILFSDKVEKFIPPQKGRRHILRIISELLNFKPEGTGTN
                     ITEALKFLTNGIKKRCTAFLLSDFLDVERLSLKPLFADALTIAGNKHDLIGIRIFDKR
                     ESDFPSVGLVKVRDNETGKYIWVDSNAPAVRSNFSNRWSQLSDNLKSTFTKASVDWVS
                     IRTDEDYVKPLISLFKHRS"
     gene            35374..36399
                     /locus_tag="PK839_01005"
     CDS             35374..36399
                     /locus_tag="PK839_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG71342.1"
                     /translation="MFRRFCLLVVSFLFIYVKSGFPQPQETEPSITSRLQRDTILIGD
                     QITFSIEVKSIKPLRVVFPNFTDSLGNGVEVVGIPTADSLAKANYHHYSMNMLITAFD
                     SGTLVVPSIPVVLASGHTTDTLFTQPHALHVNLVPRDTTLKEIYDIKPPIAEPLTFAE
                     VAPLVGSGLLLAALVVLLVLYVRSRKQNKPFLNLFKPKEPPHLVALRELEQLRNEKLW
                     ANENHKLFYTRLVDILRKYMEGRFGINAPEQTTSEILSELERIEYDFGHLKEELRELL
                     VISDLVKFAKHVPLIQENEKNMNFAFDFVNSTRPVETPEIPATTDDTGQQLQDAQSEN
                     QTTNKLS"
     gene            36410..37393
                     /locus_tag="PK839_01010"
     CDS             36410..37393
                     /locus_tag="PK839_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOG71343.1"
                     /translation="MVFAAPKMFYLLLLVPVLVAWYIFRQKDSRASLHISTSIGFENV
                     KPGFKVYMRHAPFILRMVAIILIITALARPQSTNIQKNISTEGIDIVLSMDVSGSMLA
                     RDFKPDRLGAAKEIGIKFISGRPNDRIGLVVFAGESFTLCPLTTDHATLINMFHNVNM
                     GILEDGTAIGSGLATAVTRLKDSHAKSRVIILLTDGVNNRGEIAPLTAAEIAKSFGIR
                     VYTVGVGTMGTAPYPVQTPFGTQYQNMKVEIDEDLLTSISKLTGGEYFRATNNTTLEE
                     VYGKIDKLEKSKIEVAEYRSREEKFRGFLLAAFAILVVEFTLRRTLLQGIQ"
     gene            37503..38543
                     /locus_tag="PK839_01015"
     CDS             37503..38543
                     /locus_tag="PK839_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOG71344.1"
                     /translation="MFRFAEPKYFYLLLVIPLMVGIYLYSIHRQKKQIAIFGRTDTVM
                     GLLPNLSFTRAWVKFIILLVACVFVVLSLARPQVGSKLTEVKRKGIEIIIALDVSNSM
                     LAEDIQPNRLERAKQSIAQLVDKLTNDRIGLIVFSGDAYVQLPVTSDFASAKMFLSSI
                     SPEMVSKQGTAIGRAIDLASNSFTPQGNTSKALIIISDGENHEDDPVESAKLAAEKGI
                     VIYTIGIGSPSGAPIPIGANQNYLKDENGNIVVSRLDEETLSKIAIEGNGKYIRASNS
                     QFGLLPLFENLSKMSKTEIKEKIYSEYSEQFQYFLAFALALLLIDSLILERKNPKLSR
                     FKLVDTKNGGRQ"
     gene            38540..39235
                     /locus_tag="PK839_01020"
     CDS             38540..39235
                     /locus_tag="PK839_01020"
                     /inference="COORDINATES: protein motif:HMM:NF012725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG71345.1"
                     /translation="MKVFLMSTLMLTAVISTAQSERKFIRKGNHDYKKNDFVESEIQY
                     RNALEQNSHSFKGQFNLADALYKQNKFNESLETLNNLNLTGLSNDQKASVLHNKGNAL
                     FKQNKLKESIEAYKSALKFNPNDKETKYNLSEAIRLFQQQQQNQQDNNNKDKDKDKQN
                     QNQNQPDNPKDEQNNNPDNRDSQGNNQQQPKLSKEDAERILEAIQNHEMQIQDRLKEE
                     KAKQAKVRVGKNW"
     gene            39253..41100
                     /locus_tag="PK839_01025"
     CDS             39253..41100
                     /locus_tag="PK839_01025"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HOG71346.1"
                     /translation="MSKRFLLVAFIFVSSIIQAQNVQFEASAPNMVEVGEQFRLTYSL
                     NSKPSNFNPPTIAGFEVIAGPSTSSSTSIEIINGKVTQQQSVSYTYILEATAVGKYNI
                     PPAEAMVGKVTYQSNPVSIEVIKASSGSARQANQPQPTQPQQIAAGEENLPDDDLFVT
                     IELNRKTAYMGEPVEAAIKIYTRVNILGFEDAKFPTFDGFWSQELESSSNVQFERANV
                     NGKIYNVGIIRRYLLFPQKNEKLKIDPFELVVVYQGNAARPRSIFDEFFGGGFENYRK
                     RLVSKPVSVVVKSLPQGAPSSFYGGVGNFKINVGVDKNKLKTNEAVTLKLVVTGTGNL
                     KLMGTPKVEFPSTFEVFEPKVSDNINPSQQNASGSKTFEYVAIPRASGSYEIGPVDFT
                     YFDPSAEKYVSVQSKTVNLEVIPDSSATGAVVLTSGLSKEDVKFIGKDIRFIKTNPVR
                     LKPINSFVVGSLMYLFSYILVVILFAVFLFVFRKYRKQIQDTGFVRNKHAQKIAQKRL
                     QVAQEMLRLDEGEKFYEEIHKALWGYASDKLNLPVASLTTESVREALSGKGVSESAID
                     EFIRIIEACEYARFAPSSEHLQLETIYKDTYSLILQLEENLGKVKSRLW"
     gene            41115..41873
                     /locus_tag="PK839_01030"
     CDS             41115..41873
                     /locus_tag="PK839_01030"
                     /inference="COORDINATES: protein motif:HMM:NF019339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG71347.1"
                     /translation="MKRLLNIVLLVCAPLWSLLAQNPSQLMKQANELYTSGKFKEAAQ
                     RYEAIIGQGYTSAEVYYNLGNAYFKMKDIPAAILNYERANLLSPKDEDIQFNLDLART
                     YVVDKIEPLPEFMLVTAAKNFRSWFSSNTWAYIAFFAFLLVMVAVAFYIFTNRYTIKR
                     LTFAGSILLTIVFVISLTCSISLRNQSIQRNHAILFAPVVSVKSSPASTGSDIFILHA
                     GTKVMVTRSLGEWSEIKIADGSKGWVLSSTFERI"
     gene            41939..42478
                     /locus_tag="PK839_01035"
     CDS             41939..42478
                     /locus_tag="PK839_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HOG71348.1"
                     /translation="MRILVFCVLMTVVGTAAGQTGSFYDFTVKDIDGNDFPMSSLKGK
                     KIMVVNTASKCGYTPQYADLQALYEKYQDQNFVVIGFPANNFMKQEPGSNKEIKEFCT
                     STYKVSFPMMGKISVKGKDMHPLYGWLTSKSLNGVMDSEVKWNFQKYLIDERGILVMV
                     IDPGEKPNSERVIKWIEGK"
     gene            complement(42552..44018)
                     /locus_tag="PK839_01040"
     CDS             complement(42552..44018)
                     /locus_tag="PK839_01040"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOG71349.1"
                     /translation="MVVLLQPGETAFSQNLENFGAGIIKQEELIQTVGFLSSSRFHGR
                     LPGSMEYEQAARYVATRFKNAGIKPVGQPSMLQYFNDEVNLIHDASCFILNSNGRPII
                     PLTLGEDFVCRGFTGSGHVKGEAVFAGFGMKQPNYDDYRNINVKGKIVVAFKSAPPWT
                     SETSSWGDTSPRAKARTAKEQGAAGIIFIADPKVFPPTMIYGSIACGDGPHLFDFPMI
                     QLSDRATDSLFAELPVKAPNLYEQICQDKAPHSIELGRSVLVNVKAEYFAEKPTANVV
                     GIVEGSDSLLKNEYVVLGAHLDHVGYQGEKLYFPGANDNASGVAAIIAIAEALKKSKV
                     EIKRSIIFIAFSSEESGLRGSKYFVDHSPVDLSEVVAMLNFDCVGQGDSIAIGGRLSF
                     PKLWKKVKKIDKHTTKLLSTRTFGGGGADAEAFYRAGISTLYFNTTNGYKHLHSPSDR
                     AETINPELIEKVAKLGYLVTVDLANGRYKGERDRQRKK"
     gene            44210..44794
                     /locus_tag="PK839_01045"
     CDS             44210..44794
                     /locus_tag="PK839_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HOG71350.1"
                     /translation="MTDFLDSNLYSYLVLPFLIFCARICDVTIGTLRIIFVSKGHKKV
                     APILGFFEVFIWVIAISQIMSNLNNFACYFGYAAGFATGNYIGMLVEERIAVGKLFIR
                     IITSADGTTLVKLLNSNGFGATTVKAEGGRGGVNMVYSVINRKRLDEIQKILLDFDPK
                     LFYTIEDVRKANAGIFPESFSSKIPLFSRWRRGK"
     gene            complement(44841..45182)
                     /locus_tag="PK839_01050"
     CDS             complement(44841..45182)
                     /locus_tag="PK839_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005836465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS sensor protein"
                     /protein_id="HOG71351.1"
                     /translation="MINGIDWAIGLGCAITVSAADGTILYMNEKSQKVFEKWGGKELI
                     GQRLWGCHSEKSVETIKRLIENKESNCYTIEKNGVKKLIYQTPWFDGEELGGLVEISI
                     ELPASIPHHVR"
     gene            45279..46457
                     /locus_tag="PK839_01055"
     CDS             45279..46457
                     /locus_tag="PK839_01055"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HOG71352.1"
                     /translation="MKTYNVKSYRTPLYRDAGFLLKDIPTAQKAFSDEQSLPITPELY
                     IYSRYRNPTVVAAEEYLASIEGSQWSLLCQSGMAAIDIALSVFQRANHKCKWAFFTEI
                     YGGTNSYIDSILVSRRGVDAVRFSPSGEASSNVEFDSFMTTHKPDVVFFEVISNPMLI
                     VADAVEIIRIAKKYGAAVVVDNTFATPYLFKPLDFGADLVIHSATKYLSGHGNLSAGV
                     VSGNSPELARSAIEYRKLVGHMLSPDDAYRLHDMLKTFNLRINRHFENAATVAKFLSA
                     SSKVEKVIFPGMDYHPSHEVASKLFEGRGFGGIVTFDLKGASFEDKQKKCNEFINAVS
                     DSIPLIPTLGDADTILMPVEPVWGDKYPFPGMIRLSLGIENGQEITRVISQGLDAVGS
                     "
     gene            complement(46551..48293)
                     /locus_tag="PK839_01060"
     CDS             complement(46551..48293)
                     /locus_tag="PK839_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HOG71353.1"
                     /translation="MMNSVFLPTSLKEINALGWESADIILFTGDAYVDHPAFGAAVIG
                     RVLENEGYKVAIVPQPNWRDDLRDFRKLGRPKLFFAVTSGNMDSMVNHYTANLRLRST
                     DAYSAGGKAGFRPDYAITTYCNILKKLYPEVPIVIGGIEASLRRLTHYDYWSDTLKPS
                     ILIDSHADILVYGMGEQPIVEIARRIANGKAPSELKNIPQTAFFVANIPEVAADSSLI
                     ELHSYEDCIKSKKYFAENFKLFERESNKLVQKTIVEPVGDGFVVVNPPYPPPAVGEID
                     KWYALPYTRLPHPRYKGKDIPAWEMIKFSVNIHRGCFGGCSFCTISAHQGKFIASRSE
                     KSVLREVEQVIEMPGFKGYLSDIGGPSANMYMMGGIKREKCRECTRYSCIYPSICLNL
                     NASHSAANNLYIKIRSMHGVKKAFVSSGVRYDLFTGRNTQAEKDYFENLCRFHISGRL
                     KVAPEHTSEKVLKLMRKPSFRKFRQFKTEFDLINKKYALNQQLIPYFISSHPGSTEAD
                     MQELAREIKELNIRPEQVQDFTPTPMTLASAMYYTGINPYTGEKIYVARSRKEKLKQN
                     SYFFWYKAQNTKGS"
     gene            complement(48297..49505)
                     /locus_tag="PK839_01065"
     CDS             complement(48297..49505)
                     /locus_tag="PK839_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008760889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HOG71354.1"
                     /translation="MMFTPGNISISDFEYSLPTDRIASHPLSKRDESKLLIYRDGEIG
                     SDVFKNIGNYLPEGSRLVLNNTKVVSARIIFLKETGATIEVFLLEPVFPADYSVSFSS
                     HEPVVWKCMVGNQKRWKTDTLERTVPQTDTKLKVRKSKKVSDGFEVEFSWTNTDLCFA
                     QVVELCGEVPIPPYLNRQSEEVDKYRYQTIYAQPEGSVAAPTAGLHFTPEVFSNLKKL
                     GITPDYITLHVGAGTFKPVKADVIINHEMHTEHFLADIDLIKGMSECDNFRVVVGTTT
                     LRTLESLYWLGVKASQNQLCPPYFISQWEPYKTEPIPFKAAFYELWRHMEANGLETLM
                     ASTQIIIAPGYNVQTADALVTNFHQPRSTLLLLVSAFIGEQWRDVYAYALRNDFRFLS
                     YGDSSLLFRS"
     gene            49628..50704
                     /locus_tag="PK839_01070"
     CDS             49628..50704
                     /locus_tag="PK839_01070"
                     /inference="COORDINATES: protein motif:HMM:NF021179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2027 domain-containing protein"
                     /protein_id="HOG71355.1"
                     /translation="MKVEVGDRVRFLNEVGGGKVLRILDKRMVLVETDDGFEIPVLAA
                     EVVVINRNADSQIIGDDSLPMPTLPKRTDKHPKESNQKAGSAAGISMQVATERQGDDI
                     MLLLGFVPDSLYTLASSGLKLFLINDSSYKIFYTLSSWNEGKLNPIISGLLEADTKEQ
                     VAYLVNDEARSAKTLNLQAIYHKGMPFKAYQPEFYDVEIASLKLQLAASYVENDYFDD
                     KACLVTIADTQKEVLLERMDRESIQGIIQEKENQHVKNKPKKSLPVNEIEEVDLHIEE
                     LVDSWSNLSPGEILNIQLARFETALQGGIKSNTKRMVFIHGVGNGKLKYEIHKLLDTR
                     FAGLKYQDASFKEYGFGATLVYLK"
     gene            50720..51101
                     /gene="rnpB"
                     /locus_tag="PK839_01075"
     ncRNA           50720..51101
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PK839_01075"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            51860..52324
                     /locus_tag="PK839_01080"
     CDS             51860..52324
                     /locus_tag="PK839_01080"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOG71356.1"
                     /translation="MEMKDRIRLLLDAEKINSAKFAEAIEVQRSAVSHILAGRNNPSF
                     DIIQRIAQKFPKINLEWLITGKGSMYKIPVQRSIFDTDDSINQPGKTAPTANLTESRT
                     ITNVTNRGEGGKILSGRYEGADSEDTSKELTVQKIVIFYSDRTFVEYRPSIS"
     gene            52367..53422
                     /locus_tag="PK839_01085"
     CDS             52367..53422
                     /locus_tag="PK839_01085"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HOG71357.1"
                     /translation="MYKSIFRKLLFIFPPETVHKLVVSLIKFFFKIPGARNAFRRIFS
                     NRNEACLEREVIGLKFSNPVGLAAGFDKNAEFFNEFSSFGFSFIEIGTVTPEPQPGNS
                     RPRLFRLLPDNALVNRMGLNNKGVNYVKSRLQHKENQGLIIGGNIGKNSSTPNSNAVS
                     DYIACFNELYSFVDYVAVNLSCPNVHNLSDLQNPDSVKLIIESLVNERAKFPSKKPIL
                     LKVSPDLSPEQLNQTIDAAEEAGVDGYIATNTTTGRNNLKTKKSELEKIGRGGLSGKP
                     LRDVSTEVIRFINQKLDGKKPIIGVGGIFTPMDALEKLEAGASLVQVYTGFIYEGPFI
                     VKRINKLLINKMFNTYI"
     gene            53429..54049
                     /gene="yedF"
                     /locus_tag="PK839_01090"
     CDS             53429..54049
                     /gene="yedF"
                     /locus_tag="PK839_01090"
                     /inference="COORDINATES: protein motif:HMM:TIGR03527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HOG71358.1"
                     /translation="MAKLVDCRGMVCPQPLVETRREIKKSNPGEAITVVVDNSTSLKN
                     ILRFLEDNGVECVVKQEAESSSITFNVPLQMTQPDKRAEEYCDVSPKPDVNSGFIVLL
                     NSATMGKGNDELGKILMKGFLSTLLELKATPTEIICYNSAVTLALKSSDTAATLQQIS
                     ERGIKITLCGTCVDFYNLRDDLAVGQISNMLYIAERLSSGVRIIQP"
     gene            54129..55253
                     /locus_tag="PK839_01095"
     CDS             54129..55253
                     /locus_tag="PK839_01095"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOG71359.1"
                     /translation="MNLYFDNSSTSFPKPEGVSQSINEYFSHGGTYGRAAYNRVIEVS
                     RKVESTRSLLANLIGTSLAPNVVFTSNATHAINLFIRGMRYPKRRVIISPLEHNAVCR
                     PLQYLFQTAGVGVDTFPHHPDGRIDIQKAAKVNFASYDLGIINHVSNVNGVIQPIKEI
                     KRLMGNTPLLVDASQSAGRVEIRADEWKLDALALTGHKGIMGPTGTGALFIRNPDMAD
                     PLIFGGTGSNSESYLQPIDLPDRFEAGTPNIVGIYGLWGALSSGMGYSFKREHFVELL
                     HEISTFSSFKLLAADDTEFQSDVFSIKPLGMEVSELARILFEKFSVETRSGLHCAPLA
                     HRTLGTFPTGAVRFSLSKFHTDNDLDYLHSCLKKIDEGRS"
     gene            55237..55476
                     /locus_tag="PK839_01100"
     CDS             55237..55476
                     /locus_tag="PK839_01100"
                     /inference="COORDINATES: protein motif:HMM:NF023251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HOG71360.1"
                     /translation="MREEANTGFTILIFSNIHQVMHAEKIIADKGYPYRIVPVPSSLS
                     SECGLCIRIESSYLDSILSDLQNIKVEIHTLNNEP"
     gene            55506..56543
                     /gene="selD"
                     /locus_tag="PK839_01105"
     CDS             55506..56543
                     /gene="selD"
                     /locus_tag="PK839_01105"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018359714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HOG71361.1"
                     /translation="MMEEFDLLSTVEQGGCSSKIPAALLESLLPALPKLVDENILVDF
                     SSHDDAGVYRISDDFALILTTDFFPPVCSDPFEFGQISAANSISDVYAMGGEPFLALN
                     ISMFPSSRIPIDVYKSILEGAASKALEANVAIIGGHTIDDFPPKFGLAVVGRVDPKRL
                     VTNSGLRPGDKLILTKPLGTGITVAAKRLKMITDESYRIAIQWMKTLNDRACSVMKKY
                     HVTGATDITGFGLLGHLLRMARSSRVNISIQAGTLPVLPGVTSLVNDGCIPGAAFRNL
                     EYVENDVTFTRVDYDLKMIMVDAQTSGGLLMGVQDEFANQTLQELTDAGLCYSRIIGE
                     VGEGNGKIFVS"
     gene            56733..57623
                     /locus_tag="PK839_01110"
     CDS             56733..57623
                     /locus_tag="PK839_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG71362.1"
                     /translation="MKLTKDSWITFTIERLVEIPQAGYYYRLLHESGQKFLLPAQYYT
                     HYNLVVGSTVSCRVDKINCMGKVFLEPKHPYYEVNMVYNFDFVAMNNSFVKGVAELVV
                     RDIFNETYTLFLPAEVCSKISNMVPLRVLNIRKGRLQLQLAGATGSCKEFEENEIIDL
                     TIETVVEINGEKFFFLSHSSGCFALLKVKYYLHYNFEVGKQVSCQYIGIKPDGTLRVE
                     PPNPFYRVGDTYNFTVSSVGTESNSDGETASYATVFDIYGMKCGFVVPAGSKVQPDQV
                     ILCKVVGYKKGRPMLMIVQK"
     gene            complement(57647..57979)
                     /locus_tag="PK839_01115"
     CDS             complement(57647..57979)
                     /locus_tag="PK839_01115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG71363.1"
                     /translation="MTKKRVVIKFGDLSPELLELFKQKYPHGYQNHVIKVEKPGGDYF
                     FAVTLDSDDASYLVKVNVKIDSKIKDEEDEKEFFGGLSDDIGSENDAFPDDMNEEDEP
                     SEEMSDDL"
     gene            58182..58646
                     /locus_tag="PK839_01120"
     CDS             58182..58646
                     /locus_tag="PK839_01120"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOG71364.1"
                     /translation="MTCVVPILFGYNAMAQVQIYNPNADAIKDLNQAIERAGVQGKHV
                     LVQVGGNWCPWCVKLNKIFTTDSTIMKVLNANYVLVKVNYSKENKNEAVLEKLEFPQR
                     FGFPVLVILDDHGKRIHTQDSGLLESGDGYDTKKIVSFLKNWTKAAIGKPAN"
     gene            complement(58716..60026)
                     /locus_tag="PK839_01125"
     CDS             complement(58716..60026)
                     /locus_tag="PK839_01125"
                     /inference="COORDINATES: protein motif:HMM:NF024806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG71365.1"
                     /translation="MKRLTTVLLFSVLGMGMLNAQTGVQINFDQFGNKIEKSNATIQD
                     PKKNAKPDAWIARAELFMDVFDAQIYRAYPGMDMKTFSLLIGKPTEQTQEVVEGITVD
                     KFVMDRAEFYFANGSLDHWQVTKPVVENPLEVAYESLIKAIELDASKKKAKPIMEDLL
                     KLKNYLINEGSSCYSKKDYGCSYKCFSKVVEIGKNPLVNQKDTAIYYYAGLSAQLDGK
                     FEECIPYYEESIKLGFTSEGSVYFNIFDAYKSLGKADEGAKYLEDGFIKYPKNTNVLF
                     SLISYYIDKGEDPSKILVYIDQAIASEPNNPSLFFAKGTLHDRLGDTDEALTSYQKAI
                     EIDPNYFDAYYNIGALFFNVGVKYVEEANKIPAKEIEKYDELMSKANVEFKKSLPYME
                     NALKINPASKEVMDALKNIYFRFRNESPEMSAKYKEIIDKLNNM"
     gene            complement(60088..62580)
                     /gene="gyrA"
                     /locus_tag="PK839_01130"
     CDS             complement(60088..62580)
                     /gene="gyrA"
                     /locus_tag="PK839_01130"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOG71366.1"
                     /translation="MSETERIIRINIEEEMKSSYIDYSMSVIVSRALPDVRDGLKPVH
                     RRVLFGMSDLGLNAGNPYKKSARIVGEVLGKYHPHGDSSVYDAMVRMAQDWSMRYPLV
                     DGQGNFGSIDGDSPAAMRYTEARLAKIAEDVLADLDKQTVDFNLNFDDTLEEPSVLPT
                     KIPLLLVNGTSGIAVGMATNMPPHNLGEVVDAICATIDSPDITTDELLKYVKGPDFPT
                     GGVIYGTQGVKEAYRTGKGKIVVRSKIEIEQTESGRSKIIVTEIPYMVNKAEMIRRIA
                     DLINEKKIDGISYINDESDRDGMRVVIILKKEAVANVVVNNLFKHSQLQSSFAINNIA
                     LVDGRPKLLNLKDLIVHFIKHRHDVIIRRSRFELEEAEKRAHILEGLLIAIDHIDEVI
                     NLIRSAQSPAEAKEKLVERFGLSDIQSQAIIDMRLRSLTGLEREKVKEEYNELQKLIA
                     YLKQVLEDKALQMKIIKDELVEVKQKYGDARKTEIIPDAEEFNPEDFYADEEVVITIS
                     HMGYIKRTPLYEYKLQSRGGVGAKGSDTRDEDFIEHLYVATMHNTMLFFTENGKCFWL
                     KVYEIPEGTKSSKGRAIQNLINIENDDVVRAYINVKGLDDTDYLKNNYIVLCTKRGII
                     KKTPLEAYSRPRQNGVIAVTVKEGDQLIEACLTDGKSHILIAARNGKAIRFPEHLVRP
                     IGRTGSGVRAISISPDDEVVGMVCVEENASKDILVVSENGYGKRSRLEDYRITNRGGK
                     GVKTINITPKTGKLISIKAVDENNDLMIINRSGLTIRLKISELRVMGRNAQGVRLINL
                     KKNDSIAAIANVESSENEPDEQNLVQEGESEQ"
     gene            62789..65338
                     /locus_tag="PK839_01135"
     CDS             62789..65338
                     /locus_tag="PK839_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HOG71367.1"
                     /translation="MNQKFSQKIRDVLSYSREEAIRLGSSAISSDHLFLGILRDGEGE
                     AINILLAEGVNLSELKKELDVHLLQIPSKAEEINDSSEIAIYKSAERALKIVILEARA
                     LKSESINTAHLLLAILKDDTNYVTQLLKEKDIDYERVRKNLTILNSDDKGDFGSGDED
                     EGDSGFFGNMGKSNQPSTGKSASDTPVLDNFGIDLTKAAEDGRLDPIVGREREIERIA
                     QILSRRKKNNPILIGEPGVGKSAIAEGLALRIVKKKVSRVLFNKRVITLDLASIVAGT
                     KYRGQFEERMKAILNELSRTPNIILFIDEIHTIVGAGGATGSLDAANMLKPALARGEL
                     QCIGATTLDEYREHIEKDGALERRFQKVMIEPTSPEETIEILNNIKDRYEEHHNVTYT
                     PQAIEACVKLTMRYISDRNLPDKAIDAMDEAGSRVHISNIRVPDTIVNLEKLIEETKK
                     EKIKAVKNQQFELAANLRDKEKKLIEQLEEEQSRWEKELAKHREIVDEEKVAEVVAMM
                     TGVPVQRIAQAEGARLLKMADELRNSVIGQDEAIQKVVKAIQRNRAGLKDPNKPIGTF
                     IFLGPTGVGKTQLAKVLAKYLFDTTDNLIRIDMSEYMEKFSVSRLVGAPPGYIGYEEG
                     GQLTEKVRRKPYSVVLLDEVEKAHPDVFHILLQVLDEGQLTDSLGRRVDFKNTIIIMT
                     SNIGSRELKDFGQGVGFTTKAKEQSSSSDYSRSIIEKALKKAFAPEFLNRIDDIVMFN
                     SLSRADIHKIIDIELSGLYERIRNLGFTIRISPAAKDFIAEKGYDVQYGARPLKRAIQ
                     KYLEDALAEVLIKSDTKPGCTISVGYNKKKDEISIKVLKGEEEVTSASEEE"
     gene            65414..65914
                     /gene="tpx"
                     /locus_tag="PK839_01140"
     CDS             65414..65914
                     /gene="tpx"
                     /locus_tag="PK839_01140"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HOG71368.1"
                     /translation="MNVTFGGKTVTLEGEQVKVGSTAPDFTALGTDLKPVKLSDFGKK
                     VKLISVFPSIDTEICSIQNHRFNNEASSFGDKVAFIAISNDLPFALKRFCGAEKITNL
                     TTLSDHKDVDFGKKFGFLIKELRLLTRGVIILDKDNKVQYVEIVPEIASEPNYTEAIA
                     RLKQLV"
     gene            complement(66034..67347)
                     /gene="rimO"
                     /locus_tag="PK839_01145"
     CDS             complement(66034..67347)
                     /gene="rimO"
                     /locus_tag="PK839_01145"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009229376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HOG71369.1"
                     /translation="MQQPGKGKINVVTLGCSKNLVDSEVLMNQLKMGGWEVVHDSNDT
                     EAKVVIINTCGFIADAKEESVDTIMGFVDAKNKGIIDKVFVMGCLAQRYRNELQDEVP
                     EVDGFFGVSELPAVLHALNTPVFSGCLTTRLLTTPGHYAYLKISEGCSWGCSYCAIPL
                     IRGKHISRTVESLVEEATILSEKGVKELILIAQDSTYYGMDIYGERRIAQLIDRLSQI
                     KGIEWIRLHYAYPAHFPDDLIATIANNPKVCKYVDLPLQHISDRVLKAMHRGISRSET
                     IALVEKLRQNIPGVAIRTTFLVGHPGEDQEDFDELMSFVDEFRLERVGVFTYSEEEGT
                     YAQQYLADTIPPDEKERRASELMELQRNISFMINHSRVGSIIRVIVDSVEDEIIICRS
                     EFDSPEVDQYVNIHLHGYSQNLKPGDFVNVKIVDADDYDLIGELC"
     gene            complement(67361..68314)
                     /gene="ftsY"
                     /locus_tag="PK839_01150"
     CDS             complement(67361..68314)
                     /gene="ftsY"
                     /locus_tag="PK839_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HOG71370.1"
                     /translation="MGIPNIFSTGKGNSRENLDKGLEKTRESLLKKVSRAVVGKSRID
                     DEVLDKLEEALISSDVGVETTLKIIQQIQQRAAKEKYLNANELNTILKTEIISLLADT
                     PVNSGAITELPSKPFVIMVVGVNGVGKTTTIGKLAYLYKLAGKKVLLGAADTFRAAAV
                     DQLTVWSERVDVPIVRQQMGADPSAVAYDTLNSAKANDSDVVIIDTAGRLHNKVNLMN
                     ELTKIRRVMQKVIPDAPHEVLLILDGSTGQNAFAQAREFIAATEVTGIVLTKLDGTAK
                     GGVVLGISDQFKIPVKYIGVGEKLADLQVFDKNAFVSSLFS"
     gene            complement(68427..68579)
                     /locus_tag="PK839_01155"
     CDS             complement(68427..68579)
                     /locus_tag="PK839_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004331849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4295 domain-containing protein"
                     /protein_id="HOG71371.1"
                     /translation="MAKKVVASMQQKGAKNFTRCIKMVKSPKTGAYTFKEEMVANTEI
                     DAYLKK"
     gene            complement(68587..68769)
                     /gene="rpmG"
                     /locus_tag="PK839_01160"
     CDS             complement(68587..68769)
                     /gene="rpmG"
                     /locus_tag="PK839_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HOG71372.1"
                     /translation="MAKKGNRVQVILECTEHKESGMPGTSRYITTKNKKNTPDRLERR
                     KYNPILKRVTVHREIK"
     gene            complement(68782..69024)
                     /gene="rpmB"
                     /locus_tag="PK839_01165"
     CDS             complement(68782..69024)
                     /gene="rpmB"
                     /locus_tag="PK839_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HOG71373.1"
                     /translation="MSRVCQITGKKKMVGNNVSHSKRRTKRRFYPNLKKKRFFLEEEN
                     RWVTLKVSAAAIRTINKNGLKATLNKAVEKGILKTY"
     gene            complement(69088..70362)
                     /locus_tag="PK839_01170"
     CDS             complement(69088..70362)
                     /locus_tag="PK839_01170"
                     /inference="COORDINATES: protein motif:HMM:NF001813.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HOG71374.1"
                     /translation="MNAGIITIGDELLIGQVVDTNSAWIATRLNSLGFAVERILSVSD
                     TEQAICESVNEAIDRYDLTIVTGGLGPTSDDVTKESLRRIFGGEMITDAQSLELIEIF
                     CSKRGIAVTETNRKQADVPSSCTVLPNYFGTAPGMLFKKGIHMLVSLPGVPFEMKALF
                     ENEVTPLLKQLPVEGVIIHKVIHTFGIAESNLSDVLNEFEKSISPSIKLAYLPDPSGI
                     RLRLSAYGNDRKLLSQQVDEAIEKLRATIPQYIFGYGETSLPEVVGKMLLSCNATVSV
                     AESCTGGKISHLIVEIPGASSYFNGGVVAYSNQVKENLLGVKHQTLIKYGAVSREAVE
                     EMAVGSLNLLSTDYSIAVSGIAGPDGGTPDKPAGLVWIAAASVNSLEVEKYIFSNDRS
                     INIQRASYSALNMLRKLLLKEHPELKNIFEKV"
     gene            complement(70422..70497)
                     /locus_tag="PK839_01175"
     tRNA            complement(70422..70497)
                     /locus_tag="PK839_01175"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(70462..70464),aa:Thr,seq:cgt)
     gene            complement(70741..71727)
                     /locus_tag="PK839_01180"
     CDS             complement(70741..71727)
                     /locus_tag="PK839_01180"
                     /inference="COORDINATES: protein motif:HMM:NF040069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6340 family protein"
                     /protein_id="HOG71375.1"
                     /translation="MGRIRFCGLFLLLFVSACIPYRQIDIEYYDSSTKISIPTGNGKV
                     LILTNYQGKSHSSGGETLAWALDSVAATEATLSLVKVLNSSPAFSQTSFSHYAVYRKD
                     TSNMILPLPWTQLKNLSEINDSAGLILSLDYMNVKPTAGSYTLWQGSVNAYHGYIYVP
                     VYCYWRVYDITNQKITNSFLHRDTLVWEADDWREVKPGDQLPGYFAAVAYSGADAAEK
                     FAAQIAPFWENSTRILYNSGSKQMVEAEKLALKGDWRGAAEIWQQVIKNGGKALPSRA
                     AFNMALANEMLGNFNVSLEWLMQAKRLNPNLEGLNDYMEIINYRIKANSTKP"
     gene            complement(71841..72587)
                     /gene="fabG"
                     /locus_tag="PK839_01185"
     CDS             complement(71841..72587)
                     /gene="fabG"
                     /locus_tag="PK839_01185"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HOG71376.1"
                     /translation="MKLLEGKTSLITGAARGIGRAIALAYAREGSNVAFTDMVEDDNM
                     KSLERELNALGVRAKGYVSDASNHENSQMVVDQIATEFGRIDILVNNAGITRDTLLMR
                     MSEEQWDLVIRVNLKSVFNLTKAVQKYMIKQKSGSIINMSSVVGVSGNAGQANYAASK
                     AGIIGFTKSVAKELGSRNIRSNAVAPGFIITDMTHQLSEEVRQDWIKKIPLQRGGTPE
                     DVANVCIFLGSDLSSYVSGQVIHVCGGMNT"
     gene            73032..75368
                     /locus_tag="PK839_01190"
     CDS             73032..75368
                     /locus_tag="PK839_01190"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOG71377.1"
                     /translation="MKVRLKLRSKLLLSVLSTAIVVFLVSFGYLTNKLEDITLRSAFR
                     YVDATTREHSNAVKANFDEEMGMARAMAQGFQNYKDLNYEEINKNSLRVLQGIAVNNP
                     QFLSVWASWELNSIDRTWDKPYGRVRYTYFWENGALKYVEDRVNLTGDDPNSSYYHIK
                     STKREFIIEPYWFTYTGTNMKVLETSICVPLVDNDRFVALFGFDIELERYHEFIDSVE
                     IFPGSNSMLISQDGLIVAHSDSSLTGIRVDSIFNQQKIGFNIIDKLKKESDFSFNVNM
                     NGSEYYASFQSFAISGTTNRWTFASLSPLSIIVEESKDVATRSRIVILAGLLILALVV
                     WIISYSITHPLVKATRTLRELAGGEIDTSKKLAINSGDEVEDIGSSINILIDSLYKTT
                     NFAREIGKGNFNVDYSKLSENDKLGEALLDMRKSLEHARLQEVERKLGEDKNRWANEG
                     VAKFAEILRQNSDDLNEFCYSFIYNLVKYVDANIGAIYLVNNDNPNEVYFELMATYAY
                     ERRKYEEKRIDFGEGLVGRCAKEAETIYMTDLPKDYIKISSGLGEESPTCLLLVPIKL
                     NENVHGVIEMASFEPIEDYKIRFIEKIGESLAATISNVKINIRTGKLLDESRVKSEEL
                     ASQEEEMRQNMEELQATQEEAARKTAEMESLIGALHASSFVIEYDLNGNITSVNQAYL
                     TLTGQSENEVIGTHHSDNLEMTDEQKKNYQKFWNDLRNGMVKKETSRIKLGKKTLTFI
                     ETYSPIFNQNRQVVKILKIAHNITDFIEDEKVKGRKTK"
BASE COUNT        21393 a        16049 c        16413 g        21568 t
ORIGIN      
        1 cctttatcct tgtattatcc tttatccttg cttttgctcc cttcagaatg atactcattt
       61 cggcagcatg ctggaattgg atttgtctaa cgcacggagt ttttacatta acctccttaa
      121 gttcaaatgt atcatggtca aatctggcag ctacatgatc ggtttcatat attagttcac
      181 cagctgtttc ggaaagggtt ttaatccctc cttcattttt gtatatcaac acatttctat
      241 cttccagttt tgagagcatg tagccctcat cgctgctatt gaacgacttg gtagtgaggt
      301 gaagttttgg cttgttaagg tagggtgcgc tgctggtagg acaaaaggtt gcacagttgc
      361 cacactcgtt acagaagttg gcaatgttta aaatttgata ctgctgctta acctcaaaca
      421 ccccatcggg ttcaaacttt agctgtccat cttcgccaac caacaccctt tgcatgttgt
      481 actttacggg cgaaatatcg taggagaaat tggccaggtt ggggcacacc gttgtgcaga
      541 tgttgcaaat ctcatcgcac tgaaggcacc tgcctgattc tgaaacaatt gattgttcgt
      601 caagagtttg gctaaccagg ctgaatgtac gactctcgga tggtgatagt tctggaagct
      661 caggggcata cgaccgtttt gcccttttca taatgagctc ctctttagaa tggctcctgc
      721 cggttggagt ttttatgctt ctttgaatac cggcctggtt gattatttgc tctgctactt
      781 tccgtccatc accaatggcc ttgattgccg tagcagctcc gcgtagggca tcaccgccaa
      841 tgtaaacatt atgtagcagc gttttatagg tagttgtatg ggcccgtagg agttctttgg
      901 ggacaaaatc aatatctacc tcctggccta tagccggaat aaccgtatcg cagggtatgg
      961 taaattcgga gttattcacc ctaaccggct tggggcgacc ctttgcatcg gttccctcaa
     1021 gtttcattcg gctacatacc aacccgcaaa cgttaccctt ttctataata agcctttcgg
     1081 gagctgacag ctcaacaatt tcggctccct cagccatcac cgctttaatc tcaccttcat
     1141 cagccggcat ttcgtcaatg gttcgtctgt aaacgacggt taccttgccg ttggttccta
     1201 ccagcctatg agcagttcga gcagcatcca ttgcggtgtt tccaccgccg attacaacca
     1261 cattttttcc cactcccttg atgttccctg ctttaacatc cttcagaaaa gctaacgagt
     1321 cgtaaactcc ggctgcctct attccatcaa tattcaaagg ggttgctttt tgtgctcctg
     1381 cagaaatgta aaggaaatcg tacgtgcttt ttagcttgga aaattcctcg ctggtaatgc
     1441 gatggttata tgcaatagta acacccagct gcaggatgcg cgcaatgtcg cgctcaagag
     1501 cctcgctggt taatcggaag ccaggaatgg cgtatcgaac cattccgccg gctttagatt
     1561 ccgattcgta aacatcaact ttaaaaccag cttgcgccag gtagtaggcg cacgttagtc
     1621 ctgatgggcc cgctccaaca atggctacct gctttccgtt caaaggcttt tggcttagct
     1681 taattgaggc attctcagca acaaatcgtt ttacctccct gattagcaaa ggattgtcgt
     1741 agttaatccg tgtgcacttg ttctggcaca aatggtcgca aaccattcct gttacggatg
     1801 ggaaagggtt tgtttgaagg attacctcta gggccttgtc aaattggctg ttggcagcgt
     1861 ggtgcaggta atcgggaata tcctgattgg cagcgcaagt atcacggcat ggagccgata
     1921 tgcaatcgaa gtaatcaagc tccctggtag ttttaattgt cggctcatgt aggtagctac
     1981 gctggtagcg tttattttca ataaccctgc cggcgtagct gttcaaatag tcggtaggcg
     2041 cggctccatt tttcaggtgg gcaagcccgc caacactatc aagcttgttt ttcaatagtt
     2101 ctgctgaaat agcctcaatg tactggttta acctcatgta gccacccggt ttaagaaggt
     2161 cggaacttac tgtaacagtc ctgaagccgc acttcagggt atcaactacg ttgaaggcgt
     2221 caacgcctgc tgagaatgag ataagtagct gcccgtcaaa ttcgttttgc agcttgctgg
     2281 ccaggtttat tgagatgggg tgtagcgcac gtccgctcat gtacatcatt tcaacttccg
     2341 acttgaaagt gccgcgattg ttaaccgatt ccagcgtgtt ggtgagcttt agaccgaatt
     2401 gcaaccccat tctttctgca tctccctgta gcgaacgaat aatttgaact gcatcggggt
     2461 acttaaggtc atgctcaaag gcagcatcag gtacagaagt cttgaatttc agctcatcat
     2521 tcagtatcct tctaagttcc tggcggccga gtagtgttgg attcaacttg acaagagtgt
     2581 gcaactttcg ttcagtgagc agatagcggg caataccttc tatttcattg gcagggcatc
     2641 cgtgcatggt tgatagggta atgctgttcg aaagctgggg aggaattaaa atgctgtcaa
     2701 ttgccgggta gatgggccta accaaattaa tttttgtttt aagttctgtt tcgcagctgg
     2761 tcatcctgtc gagaaaccac tgaacgtttg gcttttttat gccctccagg ttgtagccaa
     2821 cgctcatgtt aaaaatagtt tgcggacttg agccaagccc gagcttatgg ttaaggatat
     2881 gaatggctac ccaggcattg aggtactcgt taaaactttg ttctacctta agctcctgcg
     2941 accactcaca gttgtacccc tcgtcctgca tgtcgatgca tggttttgaa acctccagct
     3001 cgtcgagcgt ctggatggtt ttgagctcta tgtacctgca ccccataagc cacgcagcaa
     3061 ttatgttctg cgccatttgg gagtgtggcc cagctgcaac cccaaggggg gtagctattt
     3121 tttgcccgaa tatttccgtg gccaaccggt tgcttttttg agggttgaag aatagctttg
     3181 atggaatccc caaaattgat tgattgctgt ccagctcgtg cagaattatg ccaagtagct
     3241 gctctattgg aatcgggaaa aatttatcgc tcatatcaca tgattattac ccacaaagct
     3301 aaaaaataaa cgttgaatga gctatttttg cccttaatca agctgaataa ttagaaaaaa
     3361 tcgacagttg accatcaagg taaagaaaaa tacatcggca attatactag ctgctggaaa
     3421 atctgaaagg atggggtacc ccaaatttat gcttaggtgt gccgatggcc aaactttttt
     3481 gcaaaaattg gtatcggggt ttttgttgtt tgggtgcagg caggtggttg tagtgttgaa
     3541 cgagcatgaa taccagaagg tattactcca aatccccgaa tttcccagcg aagtgctgct
     3601 ggttgagaac agccacccgg aatggggacg gttttactcc cttaagctgg cagttgatag
     3661 aattaaaccc attgattata tatatgttgg caacatcgac aacccttttg tagatgctga
     3721 gttacttggc ctgctttacc attcagcagc acaggccgac tatcttatgc cggagtataa
     3781 gggtagggga ggacacccca ttctcatctc tcagaaaatt gctgaggata tcaaaacacg
     3841 cacaacttac gactaccacc taaaggagtt cctttcaagc tattctagct tgcgggttga
     3901 ggtgaacaat ccgcttgttc tggctaatat caataccatc gacgattacc gtcagtattt
     3961 tggtgattgg aagctgagca aataccaagc accaatccaa aattcttatg atggttttga
     4021 tgtgaaaaag ttaacggaat aggcagccta aaggttattc cagttgcccc atccttttac
     4081 attacttcac aaataaacat gttaattctg atgccccacg ggtgaaaatg ggatgggggc
     4141 agggtgctag tacaaacgct gggatggtgt gtcctgggat ttgcactgag atttccagca
     4201 gagccgcctc gttgaaaatg gcaaccattg gcacgataat aacagcactg ggtgatcgga
     4261 tttttatcac ggcgcatata acgactgcac gtcataatat tcccttttcg cgccatttgt
     4321 agctggccga aatgaggatt accagcccga tggctgtggg gatgctacca ctgaacggaa
     4381 tttttaggtt atgcaggaag ctacctagaa ctatttccga tgatgcccac attgtgccaa
     4441 gctatgaagc cctaacttac ttgctactta gctgcatgga agtgttttgt agccagctgc
     4501 tagctaaaaa agtggcggat ttttcccttt gggccatgct gtaatgaaat ttttatcttt
     4561 taaccagcca agctgccatt actattgaat tgcctattgg gttgataaat aaaattgatt
     4621 gcagtcctac tttgtaggaa gacatagcgc tgatttttgt ataaatgctg agggtaaagg
     4681 cataccgtac accatgtttc gaagaaacat gccgaacatt tcggagtcgg ggtatttggc
     4741 ttgttcgcca acagtaatta tttctccaac cccgatgcgt tggcactgac cctgcttatt
     4801 aaatatttcg tggggtagcc ttaagctacg aattcgccag tcaatcagcc caagaaagta
     4861 gaagaaaatc gaattacgat caaaaaaacg tatgggtaag attggaacct gtgccttttg
     4921 tattaagcgg ataatgctat cttgccactc gcggtcgctt actcgaaggt tacggaactt
     4981 gaaatcggaa atagcccccg aagggaaaaa acccacagca tggccacttc taattaagtt
     5041 caacgtgtcg cgaatactct gaaagttaaa cggtgagtag cccttatttg catctgtttt
     5101 aggaagaacg gaaataaagt ttgaggctag tgttttgatt agcgaaagct gcttgttggc
     5161 cataatctta aaatcgggac gaattcttgc caaaatatca atcagcataa ttccatccag
     5221 gcctccatat ggatggttgg caatggtgat gaaagctcct ttgggcaatt tttcaagccg
     5281 ttcggggtta ccaaccttgt agtcaacccc aatgtcgcta agcagcctgc tggtaaactc
     5341 tggccctgag tagtcgaagg agtgatcata aacccagtta accttatcaa ttgcaagcag
     5401 gtgcattaag aatttcaaga acttttctcc agctttaccc ttgaaaaatc tggatagttt
     5461 agtaatgtct ttttcgtcaa tgagcttatt agtcgactgg gcggtgaggt taggcttcat
     5521 tttagaacaa aatcagttag aggaaaacaa aaatacacat attatctgca tttggaaaat
     5581 ggacaacgag tgtgtaagac ttccgcttaa ctttcgactc agcctgatat ttgggcaata
     5641 cagccttgct cattggttga tgaatacgat ttctaggagt acagccccat cagcaccttt
     5701 tcaggtgtaa ggggtaaatc gtagctgggc tggtagctag ggttgaactc cataatggca
     5761 ttctgaatgg cgaagtaggc ggcaattccg tacatgaagg gtggttcgcc aatagctttc
     5821 gatttcataa tggcctgctc tggaccatcc gcttcaagcg gaatgcacct gatacttttc
     5881 ggggcaaaat ggatgtcagg aaccttgtag gttgataggc tattggacag gagggttcct
     5941 ttgttgttga acataagctc ttcaatggta gcccatccaa ttccctgaac aatagcgcct
     6001 tcgagctgtc cggtatcaat ggtggggtta aggctattgc caaaatcgtg aactaccagc
     6061 acctcgtcaa tagaatatgt gcccctgata cagtcaacgg tggtagtaac gatgcctgtc
     6121 ccgtaaacca tgtaggcaaa aggatggcct ttctccttgg tcttgtcaaa gtatattttt
     6181 ggggtagagt agtgcccgtt ttcggtgagg catatccttt tcaggtaggc agtttgaatc
     6241 aacttttgcc aggatgtact gctgcgctct ccccgacata aaacttcctc atttttaatt
     6301 tccacctcgc tggccctgca gctgagcatt tctgcggcca cctgctttag cctttcaaga
     6361 agccgggttg cagctatcag cagggcattt ccattgatat cggcacctgt acttgcggcg
     6421 gtaggcgacg tgttggcaac cctggatgtg ttggtagagt atattttcac cctttcaatt
     6481 cctatgccta aagtaatggc agcaacctgg gcgagtttgg tatttacacc ctgtcccatc
     6541 tctacagctc ctgtgctaac cgatacgctt ccatcctggt atatatgcac caaggccctt
     6601 gcctggttca ttgttatgtt ggtaaatgat atgccgaagc acaggggcat gacggccatg
     6661 cctcgcttgc tgaacctatt tttgctgttg taaagtttaa tttccttttg gagcacttcg
     6721 attttgaact gttcttttgc cctttcaaag cattttgtag cataagcgtt ctcggccacc
     6781 tgtccatact gaaactcgtc tttttcacta atcaggttca gctcctgtat ggtttgtgct
     6841 gctacaccca gctcgtgagc agctttagca atggccgatt ccattacaaa cattccctga
     6901 ggtgccccaa acccacgaaa tgctgtgttg ggtggcaggt ttgtccggca gctgtaaacg
     6961 gttacccgcg tattggggat aaagtaggag gcagttgaat ggaatagcgt tcgctccatg
     7021 atggcgggtg atagatcggc cgaagcccca ccgttctggt aaagagtggt ttcgtaggca
     7081 ataattttaa ggtttttgtc gagtccaatt ttaaaatcgg cgctgtaggg atgtcttttc
     7141 cctgtcatcc gcatgtcgtc gtggcgcgag agcgaaagct ttacaggctg cttaagcagg
     7201 tgtgcggcca gtgctgccag tgctgcaaac tgcgaaggct gatcctcctt gccgccgaat
     7261 ccacctccaa tgcgttgaac gtcaacttca atctggtgca tgggcaatcc caacaccctg
     7321 gcaatggttc gttgaaccgc tgtaggcccc tgggttgatg agtgaactcg tagaaatccc
     7381 ccctctgttg ggtaagcgta agcaccctgc gtttcaatgt ataggtgctc ctgtccgcca
     7441 atttccactc taccatcaaa tatatgggtg catttgcacc atgcttcatc ggtgtttccc
     7501 atgctaaagg ttcggggcgg aatgatgagc attccatttt tctgagctac gcggggatcg
     7561 gtaactgcag gaagttcctc gtagtcaacc ctaattaatg aaattgcctt gcgtgcagca
     7621 atctctgttg tggctgcaac aatggcaata ggctgtccct gataatgcac ctcggtttcg
     7681 gccagcaaga cttcgtcctc aactattccg ccaatctggt tctcgccggg tatatctttt
     7741 gccgtgaata ttcctacaac accctccacc cttgcagcat aagtaaggtc aatgcttaaa
     7801 attttcgcat gggccactgc tgaaccaaat atttctaaat gaagtgtccc cttcagggtg
     7861 gttatatcat ccaggtaaat tgatttgccg gttacatgat taatggagtc tatgttgttc
     7921 atcgggttca cgttgaagtt attcatccaa tgttcattca aaatatgcta aagccatgtc
     7981 acctttaaca gttcagggaa aagcttcgag agatgtgcaa aaaggagctg cctcagaagc
     8041 aaccttttgt aatcagcact ccctcttatg tcggaaattg gcgaaatttc ggcctgcatg
     8101 atggtgttgg ccttctcaaa agtctcctgg ttaatggttt ttcctaccat gaacatggag
     8161 gtattaaaaa gataggtggg tattggagcc acccctcctg ctgaaacatg acagcttgtg
     8221 atgacgttcc cctcagtaca ggcaagaatg gatgagttga cgctggcaat atcaagatac
     8281 tttcgcttgc tgattttctc aaagttgaat atagattttg gtgatggaat ctcaaagctg
     8341 atgctctcaa cacgctcgcc ttcatacaaa ctcaactttt tgtagccggt gtagaattcc
     8401 ctaaggggtt gaatcctcat atttccattt aacgaggtgg aaatttctgc atttagcgaa
     8461 agcaggatga tgctgaggtc ggcaataggg gaggcgttta caatgttacc agcaatggtt
     8521 gcactgtttc ttacctgatc ggaagctaca aagggcatga tgtttctaag ggttggaatg
     8581 atgctgcata aaaggtcaga gtgtgtaagt tcgttaaatg ttgtggcagc gccaatcacg
     8641 catctgcttc catccaattc gataccctta agccccctaa gagcgccaac tggcattata
     8701 ggatcattta gaatatgatt gggcttttgc accatcaaat cggttgcacc gccaatgtag
     8761 gtagaatttt cgggagcggt ataatcaagc ggatgaattc cgccaagcag cctcgggaca
     8821 tcaaggaagt atgctggcag gtaaccatgc tctaccatcc agcccaccgg gttgaccagg
     8881 tttttgtccc gaataatttg atttaaatcg gctgctgccc tctctattga tttgtagccg
     8941 gtgcatcggc aaatgttacc gcttacagcg tttatagcgc cttcgatgct cggacgctca
     9001 tgacttaggc agtaggaggt gaacgacatc acaaaacccg gagtgcaaaa cccgcactgc
     9061 gttgccgcat tgtccaccat cactttttgc accggtgtta gctccttatc gaggttgatt
     9121 ccctcaacgg taactatgtg cttaccatgc acgttgacca aaggggtgag gcacgataca
     9181 atgcttttgt attccacatg ctcacctgtc agttcgccca gcattacggt gcatgccccg
     9241 caatcgccct cgcggcagcc aactttagta cttggcaaat cggcctcgta gcgaatataa
     9301 tccagcaggc tcatgcccgg attcttgtaa gtttcaacca gccggttgtt cagtaaaaag
     9361 gtaaccattt tgttggagta aaaaatccct acaagatacg taatggttaa gggtagctgt
     9421 aatgaagctg gtaaaaaata gtaaaaattt ctacatattc gctaaatcat ccgaaaaaat
     9481 ataagtaaaa tttactttac ctggcatgtt tactaaattt ggtatggatt ggaaaaaggg
     9541 tggggcaacc tggtgttgaa tgttgttgtc ctactgaata tttttgctga aagaagatta
     9601 ctttccgata cagaattttg agaatatgta gcctagcacc tcatcgttag ttatttcgcc
     9661 ggttatgctt cccagatggt agagagcctg atgtatatcc actgccaaca ggtcgccggt
     9721 taggtttgat tttagtccag ataataccct agcaaggcta tcgcgtgcgt ttacaagcga
     9781 ttcgtagtgg cgtgcgtttg taaccaccac gttgttacca tcgggctgtg ctgacatacc
     9841 aatatttaca aggttttgtg ttagttcgtt tatgttttgt cctgattttg ctgaaattat
     9901 gagggttata cagtgaagat ggctttcaat gttctgggct agcagctcgg tatgttgggc
     9961 ttcggctctg tctgccttgt tcagcagcac cataagggtt tgctggttgg tgagtttttg
    10021 ctggaatttc tcaacttcgg tcaggccaac gctcatgctt tctgcggcgt caatcaggta
    10081 gagcaccact ttggcctgtt caattttgga atgagttcgg gctatgccaa gcgactcaac
    10141 ctcgtcggtg gtgtgcctga gtccggcagt gtcaatgaac ctgaagttca ttccggccag
    10201 ctgtatggtg tcctcaatgg catcgcgggt ggtgccggct atttccgaaa caattgcctt
    10261 ttcttcattc aataggcggt tgagcagggt tgacttcccg acgttgggct tccctgctat
    10321 ggtaacgggg atgccctgct taatggcgtt gcccatgctg aacgaatcgg ccaaacggct
    10381 aacctcttca tgaatgttgc caacgagggc ttcgagctgt ttgcggtcgg caaactccac
    10441 atcctcctcg ctaaagtcga gttctagctc aatgagagcg gtaaacctca gcagctgatc
    10501 gcgcagcagc ttcaacttat ccgaaaaccc tccccgcatt tgctgcaagg ccacgcggcg
    10561 ctcagcttca ctagttgacg ctataaggtc tgcaaccgct tcggcctgcg acaggtccat
    10621 ctttccattc aggaacgccc gcagggtgaa ctccccgggt ttggcaagtc ttgccccacg
    10681 gcgaatcagc aactccagaa tttgctgttg aataacaacc gagccatggc acgaaatttc
    10741 aaccgcattc tcgccggtat aggagtgggg cgcatgaaaa atgcttacca gcacctcgtc
    10801 aatcacccgt tccatatcaa caatgtgtcc atagtgaagg gtgtagggtt tagcctttag
    10861 gagacttttc ccggccatca caggtttgaa cacaccatcg gtcagggtga tggaatccac
    10921 accgctaacc ctaatcacag caatggcccc cattccgggg ggtgtggcaa tggccactat
    10981 ggttgagtcg tcgagcatcg tatatttttt gcaaagttac accatttggc atcttagatt
    11041 ttcattgcaa atgcaacttt tagggacatt agcttaaggg gcagacccaa aaaatctaat
    11101 ttttcaagaa cgagagtgaa agagctaatc cttccctcgt cgttcctgac ggttaggtgg
    11161 ctatcctcgc cccctaaatc tcttaagaaa taagctgcag gatggtacac taaacgcaag
    11221 aacaaaggta cactatttca aagatgttgg gaaccgggta caaaaaaatt gagatagcca
    11281 gggtcgttgg ggttgacaag agcgccatta cccagtaaat taagcataac accgacgggc
    11341 ataatgcgtt taccggtatg ggttagcccg gcgcaaggcc aagggtcgct agcggcggtg
    11401 ctcaccccag ggtagtagcc tatattgagc ggcggcttag ttaggatcga tggctgcccg
    11461 agcagatttc taaacgggcg aagctggaag gtattggctg cgtgtccacc actaccatgt
    11521 ttaacaaaaa agttgcattt gtaacttaga tctaccaatt actcgtgctc taagtttaaa
    11581 ttttctacct ttgcggccaa cacttttaaa tcgtttttaa aatgaatatc tacggcaaac
    11641 ttactggaac aatgatagtt atactagttg cagcatcctg caaaaataat agcagcgagg
    11701 tgaaaaaccc cctgctggct gaatttgaca caccattcca aactccgccc ttcgataaga
    11761 taaggcatga gcattatgca ccggcaattg attcggccat tgccgaggca aaggctgagg
    11821 ttgaggctat tactagtaat cctgccaaaa gtacattcga aaacaccatt gaagcgctcg
    11881 accgaagcgg taggcgtttg aacctggtgt cgaacattct gttcaacctc aatgagtccg
    11941 aaaccgattc gaccctgcag ggcattgctc gtgatgcggc catcaagctc accgagttta
    12001 gcaacgatgt caacctcaac ccagaacttt ttaaaagggt taagcaggtg tgggaaatgc
    12061 gtgactccct gaagcttacc accgaacagc aaacgctgct cagcaatacc tacaagggat
    12121 ttatcagaaa cggggcaaac ctttccgata gcgacaagga gaagtttcgt gccataagta
    12181 aggagttggc cgacttatcg ctccgtttta acgaaaacct gcttgccgaa acaaacagct
    12241 accagcttca tattaccaac gaaagcgact tggcaggact tccggctagt ttaaaggatg
    12301 ccgctgccta cacagctcag caaaagggga aaggtggatg gataattacg cttgattacc
    12361 caatgtatgg tccattcatg aagtattccg aaaataggga gctgcgcaaa caactctata
    12421 tggcttatgg tagccgttgc tttaagggaa atgagtacga caaccaggct attgctaaga
    12481 aaattgccaa cctacggtta gaaattgcca acctgttggg ctatcctaac tatgcttcgt
    12541 ttgttcttca ggacagaatg gccgaaaacc ccgaaagggt gaataacttc ctgaaggagt
    12601 tggttgatgc ttctatgcct gctgccagac gtgaagttga agaggttcgg ggttttgcca
    12661 aagagctggg ttttaatgaa aatattcaac cctgggattg gtcgttttac tccgagaagt
    12721 tgaaaaatag tcgctatagc tacaacgagg aggaacttaa gccatacttt gaactcaata
    12781 gggtgattaa cggtgttttt acacttgcca acaagcttta cggcttaacc tttacaccca
    12841 acaaccaaat accggtttac catcctgatg ttaaggccta cgaggtttat gatgccacgc
    12901 ataagttcat ggcggttctt tacctcgact ttttcccccg tgaagggaag agcggtggcg
    12961 cctggatgac ttctttccgt tcacagtata gggatgggag cactgacgtt agaccttttg
    13021 tttcgctagt taccaacttt accaaaccca cggcaaccca accctcgctg ctgacatttt
    13081 acgaggtttc aacattcatg cacgagtttg gccatgccct gcacggaatg ctttcggaat
    13141 gcacttactc ctcacttagc ggaacatccg tttaccgtga ttttgttgag ctgccatcac
    13201 aattaatgga aaactgggcg gttgaaaagg aattcctcga catgtttgcc agccactacc
    13261 aaaccggcga aaagataccc caggagctgg ttcagaaaat tattgatagc cgtaacttcc
    13321 tggcgggcta cagctcaatt cgccagctca gctttggcgt gctggatatg gcttggcaca
    13381 ccgttgaaaa acccgtaacg ataagcgttg gtgagtttga aaaaagagca attgccaacc
    13441 ttgaagtttt acctactgtt gagggaacca accagagtgt gggttttgga cacatatttg
    13501 atggcggtta tgctgccggt tactatggct acaaatgggc agaagttctc gatgccgatg
    13561 cctttgaaat gttcaaggag catggaattt ttaacccaga agttgctgct agctttagaa
    13621 caaacatact atctaaaggt gggagcgagc atcccatgga gctctacaag cgctttagag
    13681 gacacgaacc aagcatagat ccactgctcc gaagaagcgg attgaaaaat taaggtaaaa
    13741 gcagtagctt aataaaatat tataccctaa atcaacgggt ggtgttttta agcatactaa
    13801 atttacttga cggtttaaaa atctaatcat acatttgtaa caattattgg ctcccgtatg
    13861 ggattaaaag ggaatccggt gtaaaaccgg agctgtcccc gcagctgtaa gctcaatgta
    13921 atggcctttt tacccctgcc actgaccgcc aacctgcggt tgggaaggct taaaagaccg
    13981 agcaagccag aagacctgcc aagttgtatt ttcgtagctt tcgggtgaaa ggcgaagatt
    14041 agaacgctgt tccattcagt tctacttttc actatttccg agtgctgcga atgattgatt
    14101 gcctattaat catttgtagc tatgcatctt aaaagggttt gctcgatcgt ttttcttcag
    14161 cttgtgctgg tcggttattt gtgtgctcaa atgtcgttat ccgacaccat tcgcatggac
    14221 gaagtgaagg tttacggcaa tttacgcgaa agccattcta taggtgccac catttacaaa
    14281 atggattctg taaagctgaa aatctttcaa aatcgatcag ctgccgagtt gctggcaatg
    14341 ggttcggtga acctcaaatc gtacggtgtt ggcggccttt catcgctaac catgcgaggc
    14401 ggaaactcct cacaaactat cgtggtttgg aatggggtga acattcaaaa cccgatgaat
    14461 gggggtgcta acctttcgct attccctgta aacatgttca gcagcatcag attgcagtat
    14521 ggcgggtcag gaaccattta tgggagtggg gccatatcgg gaatactaat tttgaacagc
    14581 aatgcattat tttctcagcc taatggtggg gagctatccc tttcatacgg aagcggaaat
    14641 acccgcagcc tttcatcaca ggttaagtat ggcgacaaaa gccgggcgat ttcggttaag
    14701 gtttttggac aattagctga taatgacttc gagtttcgta atatttacaa aattggcagc
    14761 ccaaaggaac gcattacaca tgctgagtcg aaacagctgg gaattttgac cgatgtcaac
    14821 atgcggctgc atgaaaaatt attttggtcg ttctcgggat ggggccagct aggcgacaaa
    14881 aatctgcaac ccattatgag cagcacgcag cccagctacg ccaaccaggt tgaccgaagc
    14941 ctggcgttgg tttccaacct tagcttatca ccttctaaaa ggattctttt caacctgaaa
    15001 aatgctgcaa gtttgggaac tatagactac tcagataagg gcgttaatat ttttaccaac
    15061 aacagctacg cctctattct cactgagctg gaatcaaaag tggatgttct gaataatggg
    15121 gaggtaagca tggggattaa ccacaccctc gaaacagcca gttccgacga ttatgtagga
    15181 agtgcaaaaa gaaacagagt ttcaggatat gcctcgtttc gtaagccttt gtttagctca
    15241 agatttttat cagtgctgag catgcgtggt gagttggttg atggtgattt caaacctgtc
    15301 atctattctg taggagcgga atggcttccc acaaaaaagt taatctttaa aatgaatgcc
    15361 tccaaaaatt atcgaatacc tacacttaac gacctctact gggccagcac aacatatacc
    15421 gagggaaatc ccaatcttga acctgaatcg ggttggggtg gcgatgtggg atttcaggta
    15481 aagagcatca gcagcgatag ggcaataaac gtttcaaatt cgctattcta cagcaatatt
    15541 gagcgttgga ttgtatggct cagcattcct acggtttccg atagtaaatg gaagcctttc
    15601 aacatgagca aaggcgaatc gtggggttgg gagtctcagg tcgattggag tttaagggtt
    15661 aacagcctta ctattcgtgt ggaaggattt tacactttta cccattccgt attcagtgag
    15721 caaccgggta gtagggctca accaatgatt tacacaccaa cccacaggtt ttccggctct
    15781 cttaacctac agtatgaaaa gtatgggctg ggatactatc acggttttac cggcaaacgc
    15841 tatagggatt ttcaaagcca gttgccggca tttaaccttg caaatctctt tattagctac
    15901 aagttcaacc tactttggaa ggttgtaaca gccggacttt cggtgaataa cttgtggaat
    15961 caagactacc agctaattgc cggatacgcc atgccactta ggagttatat gttcaccata
    16021 aatattgact ttaccaggta aattgattca ttaacttaaa cctttaacca tgaagaaatt
    16081 agtaaaatta atgatggcga tgcttgtgct tgcctttgcc tttgcctctt gcgggggtga
    16141 tgatgaaact cagtatgctg agtttgaaaa cggtgcaatt attctgaacg agggagctta
    16201 cggtaactcc aatgcttcga ttaccttcat tggttacggt tcagatattg ccgataacca
    16261 aatattttac cgcataaatg gccgtgagct gggcgatgtg cttcagcatg ccgctatcta
    16321 taatggaaag atttacatgg ttttgaacaa ctctggcaag gtggaggtgg ccagcagcgc
    16381 tacattcatt caacttcaaa caattactgg actggtaaac ccacgttatg tggctgcaaa
    16441 agatggaaaa atttatgtta cccaatgggg tagttggggc gagcagggca ctgtcgaggt
    16501 gttcgatgcg aatagctttt cacattctaa tactatcaag gttggaacag gcgcagaggg
    16561 tttactttgt accgatggga aaatttgggt cgccaatgga ggagggtatg gcttagactc
    16621 cacggttatg gtggttgatg ctgcaaccgg cgctgtttcg gacaccgtaa ctgttggcta
    16681 caatcccaaa gagttggttg aggatgccag cgggaatatc tgggttattt gttccggtgc
    16741 cgatatctac gatgacagct gggaagttgt aggtcatttg ccttccaagc tggtaatgat
    16801 agaccgtcag agccggcaag tagttcatag cctgattatt tccaatacca gccaccctgc
    16861 tcatatcgat gtaagttcgg gaaaagaaac cgtgtacttt ggcggaggat tcggggtaaa
    16921 tggaattttc tctgtaagca ttactcaacc cgtgattccc caaacaccgg ttattgatgg
    16981 tttcttttat gggttcaaca ttaggcccag caatggtgat atctactgca tggttgcacc
    17041 aaattttacc tcggcgggtg agctgaaaat atatagctct gctggcgctg ccctaaaaag
    17101 ttatagtgta ggaattgcac ccagcactgc cttgttcaac taatgaaata tcaggtatta
    17161 tgcaaaaaaa cctgtcgaaa gtacatgaaa cataagcgac aggttttttt aagcattact
    17221 gtgatttagg ccttcttagg ggtgaaggca caacatgggc atccataagt tcgctgtcca
    17281 gcggtctaag aatggcatca tactccaagg ttggtagctt agcatcaagc caactcttca
    17341 ccaggtcttt gtcaacaatc gcaggcattc gtttttttgt gttgtggatt tggctcatca
    17401 gctcatttgc aggttgggtg atgatggtaa aggtatcaat caactcccct gtttctacat
    17461 tagtccatga gttccatagt ccggctatgg agaatgtagc atcagccctt ggcttaatta
    17521 accaggacac ctttttcccg tttacttctt gccattcgaa aaatccggta acgggtataa
    17581 tgcacctgtt agaccttgca gcataacgga acgaaggttt ttcatgtatt gtttcaattc
    17641 tagcgttata ggttttactt ctaatcaggt tggcttgttc tggtgtggta acccagtggg
    17701 gaattaatcc ccaaaccata gggacaataa ggctactatc ccgattggct attacgggaa
    17761 ccattggatt ctcaaaggct gaggcaaagt aaaatggagt atatccatac gaagcactaa
    17821 atttcgcatg gtagcttttc tccaacgccg acttctcaat atttagattt atggaaaagc
    17881 acataggcat tttttcaatc acccaatttt ctacctttaa agcaaaagta aacgaatatt
    17941 tctgaaatca ggtttacgat tgcatttact acgaaaatac ttaagcaggt acttgccatg
    18001 ctatggagtc atgccgatga agcgaacaga attgctcaaa ttttcgtagt aatatcaaat
    18061 ttttttactt tttttgttag aaaatttggg ttaatgctta cggaattgaa acaaaaggcc
    18121 aaatcaacca tactcatagt atggcttgcc ctatgcttag tagcaattcc aagcatattg
    18181 ctttcacaaa acggcttaca aatatctgcc cgattgatta ttaaaaaggg cgaattacaa
    18241 gggtctaaga tatcaatata caaaaatggc aatcttgaaa ggcaaatgcc tgttagcagt
    18301 aagggtgtac ttgaactgac tctcgattac gactgcgact acattttttc ctttgaaaag
    18361 gaaggatata tttccaaaaa aatcgccatt gatacccgaa tacccgataa gctaaaagaa
    18421 agttttgata aggttactgc attcgatgtt gaacttttca agcaaagtga gaatgaagtt
    18481 aaggagtaca aaaatccggt agcgcggatt caattttctt cccaaatttc ggattttgta
    18541 ttcgataccg actactcctt acgcgttcaa caggaaattg cccaggcaaa caaggacttg
    18601 gtggaggaac gtaaaaagca ggaaagtttg gatttggcta aaaaagaaga ggaaaaacgt
    18661 cagcagcttt tagccgaggc tcaaaagaga gtggaagcag aagcccaaaa aaaggcagca
    18721 gaagaaaagg cacgtcaagt tgaagccgaa cagttgaaaa aggaagcgga ggaacgccgc
    18781 ttagcagaag ccaaagctaa ggaagaagct ttaaaacgtg aggaggagtt aaggttaaaa
    18841 gcacgggaag aagccaggat taaagccgaa caggaagccc tgcgtattgc cgaacagaaa
    18901 agaattgagg aggaaaagtt aaaacaggag caggagcgca ttaggcagga ggaggaggca
    18961 aaaaggaaag ccgccgagga agcccgtatt aaggaggaac aacgcatacg cgaggaggca
    19021 gagcgcattc gggtagccga agaggccaaa aagaaagccg atgaggaggc caggctgaag
    19081 gaactcgagc aacaaaaaaa ggaagctgag gagcgccgct tggcagaagc caaagctaag
    19141 gaagaagctc taaaacgaga ggaggagtta aggttaaagg cacaggaaga agccaggatt
    19201 aaagccgaac aggaagccct gcaaatagcc gaacaaaaaa gaattgagga ggaaaagtta
    19261 aaacaggagc aggagcgcat taggcaggag gaggaggcaa aaaggaaagc cgccgaggaa
    19321 gcccgtatta aggaggaaca acgcatacgc gaggaggcag agcgcattcg ggtagccgaa
    19381 gaggccaaaa agaaagccga tgaggaggcc agactgaagg agttggagca gcagaaaaag
    19441 gaagccgagg agcgtgccat tgctgatgct aagctaaagg aagaacaacg aaggattgag
    19501 ctggaaaagg cacaaaagga agctgaaaga aaaaagtttg aggaggagga gcgtttaaaa
    19561 gccgaacagc aacaaaagct gctttttgaa gagcagaaaa agagggataa ggagctgcgc
    19621 ttgaagctgg ttgccgaaaa aatgagtgct aaaatcggaa cagaggatgc cgaacgtgtg
    19681 gtaacccttc ttacccccga agatgttact aagaaatata cccgagaacg aaccgatgag
    19741 gtaattcgtc tcccaaggat tgtgattacc cgcacggttt acaaccgaaa tgataagatt
    19801 accttttaca cccgcgttga ccatgagtat ggcgcaatat tttactttaa ggacggctac
    19861 attatatcag aagagcagta taaagccgaa acaaagtagc ataattcatt ctcagccggt
    19921 aagtaagttc ccatttttta gattgtaact ataataattc gtgaaggagt ttgttacagc
    19981 atgcccacgg aactgctaca gtacttgcag cttcattgtt caagttgaaa aggacaaggt
    20041 tgttggcatc aaaccgttat ctgcaaacaa agcaacccca acgggggtat gtcttaaagg
    20101 gttaagctac gttgaaaggg caaattctcc cgataggata ctttatccct acaagaaaaa
    20161 tggcagtggt gattttatca gaattagctg ggatgaagca ctttcgacta ttgcagggaa
    20221 gctccgctat ttcaagaaaa cctttggttc gcaaagtgtt ctcttttacg ccgccagcgg
    20281 tatgtcggga cttataaacg agtttagcgg aaggttctgg cgcgaaatat acggaggtgt
    20341 aactaccacc tatggaaatt tatgctggcc agctgggctt gaagccacac ggttaacttt
    20401 gggcgagaac aagcacaacg cgccatggga tttggaaaat gccaggctaa ttgtactctg
    20461 gggcaaaaat ccagccgaaa gcaacatcca cgagatgatc cctttaagca ctgctctcga
    20521 caagggggca aagcttgctg tggttgatcc ccgccgaaca agttcatccc agcgagcaga
    20581 cctgctggtt caaccgcttc catccaccga tgctgctttg gctctagctg tggctaatat
    20641 cctcataaag aacagctgga ttgacgaaga ttttattagt agctacgtga atggatttga
    20701 ggagtttaaa gcaaaagcga tgagctgcac acccgaatgg gcttcttcaa tttgcgacat
    20761 ctcagtagaa tccattgaga agctggcatg gctaataggg aatgttaagc ctatgaccat
    20821 tattcccgga tacggaatgc agcgctacac caatggaggg caaaccatca gggcaatact
    20881 tgcactctct gtaattacag gaaatattgg taaacccggc gcttgttggc actatgcaaa
    20941 tcttcaaagc tacgtgtttg atgaagtgct ggaacccatt tgctactatc cacccgaaga
    21001 acccgacagc actacccgtc gttccatctc tacagccctg ttaggcgagg acatgctcag
    21061 cactacgaat ccccagctaa aaatgatttg ggtggaacgg ggcaacccgt taacccaaaa
    21121 tcccaatact ggatcggttt tggaggcatt ccgaaagcta gacttccggg tggttgttga
    21181 gcaattcttc accgatactg cccttgaagc cgatattgtt ttaccagcaa agaacatgtt
    21241 tgaacagtcg gatataatcg gatcctactg gaatccttat attcagttaa aacaaaaagt
    21301 agttgaaccc gcaggagagg tgaaacccga aacggaaata tactggctac tggctaaaca
    21361 gctgggtttt aatgaaaata ctcttaaaaa tattctttta cctcccggaa atgatgaggt
    21421 tgaaaaattc ttggcccaga agcttgccaa atttcccgga ttatcattag agttactgaa
    21481 aatgggtcca gttgttcctc ctggacatca ggaaattgcc ttttccgatt ttcgctttgc
    21541 gaccccgtcg ggtaaaattg agctgctttc ggaacaggtt gcttctctct ggaatgcaaa
    21601 tccactaccg gaatttttgc ctgccatgga aataagcgat gccaatagta cttacccatt
    21661 actgcttttg actccaaata caaaaaacag gattcattcc caatttggca acctgaaaat
    21721 tataaaattc atcaacggca atcctgtagt gcaaatgaac ccatcagatg ctatgcatag
    21781 aggcattaaa aatggtagcc tggtaaaagt ttacaatgcc cgcggtagta ttcaagttag
    21841 tgcacagccg acctacgagg taagaaaggg ctgcgtggtg atttataatg gttggtggaa
    21901 atctcaggga gctgcactca acacgctttc tgccggtcgc gaaaccgata tgggacatgg
    21961 ggctgctttt cataataaca gggtggaggt taacctggta aagaagtaag caatgcagta
    22021 caacggtttt atattcgatt acgccagatg tgtagggtgc catgcatgtg tggtagcctg
    22081 ttataacgaa aacggagttc aaccacctgt tttgtggagg caggtggttg gtgtgaaccc
    22141 cgaaaaagtt cccctttatg gatttgttag cttatcaatg gcatgtaacc attgtactga
    22201 tgccccatgc ctgaaggcct gccccgcttc tgcttactat cgggatcagg aaactggggc
    22261 tatacttcat agtaacaaaa gatgcattgg ttgtaaatac tgcacatggg cttgcccctt
    22321 tgatgcgcca aagtataatc ccttaagagg ggttgttgaa aagtgcacca tgtgtaacga
    22381 aaggttgaaa agtggagaaa taccggcctg tactgaagca tgccctacag gtgctctctc
    22441 atttggcagc attgaaactg cagctagcat tcaggcaccc ggcatttccg ctaaaccatt
    22501 ttctcctaga attgttacta aaaacgagga tattgtaaac catatccccc atatcgacga
    22561 acaagttacg ggctatagcc ccttgcaaca tacagggatg aagcattttg ccccttctaa
    22621 aattaacccc attcatgagt tgcctttagc catctttacg ctaatttgct cgcttatggt
    22681 tgggtgggtg ggttcgatgc catgggtagg catcgcttca agggggataa ccctgtttat
    22741 tctgttagga attgtagggg ctctactgag caccttccac ctaggcaagc cctttagggc
    22801 attacgttcc gttgctaatg ttaagagcag ctggttgagc cgtgaaatcc tgctttttac
    22861 cctctttttt tcgctgtcag cattaaacct gatttttttt aacctcatcg ttctatcagt
    22921 gattgctgtt attttggggt tgcttctttt agttgctgtt gagatggttt actccgttac
    22981 tgataagaag tataaaattc atgttcatag cgcaaatgtt gtgattacag ctctgacaat
    23041 cttttcaatt ttcaacagct ttgagcgcct acttgtccta gtgcttgttc tcaaggcagc
    23101 cttatacata cttcgacatg caaactattc tgtaagttca ttagaattta tttttagctt
    23161 cattcgggta tttctgggaa ttcttattcc cctcggctca atatttttct caaaccaaca
    23221 catttctatg ttgatgattt tgttcatttt gataggagag ctgattgata ggattgaatt
    23281 ttatggggag cagcaggtag atactccacg gttacttgcc aataactttt gggataagtc
    23341 ttccgtatag tatatatgtt gctgataaag tgtacgttac attactttgg tattactttg
    23401 agtgttaaat tttttgttga agttggattt ctttaaaatt tcctcatata tttgttacag
    23461 gagtgataag tttttttgtt ggtgataaat actagaataa tggataagtt ttatattaag
    23521 ggtactgctg aaacgcctac tgtgagtttt gattcaacgg gaaacacgtt tgaaatttca
    23581 ggtaaatcgc tgcctgaaga tgtgaaggaa ttttaccatc cgataattaa gtggcttacc
    23641 gagtactgtc aatctcctaa cgacgcaaca acattgaagg tacgaatgga gtacttcaat
    23701 acggcctcat ccaaaatgat actggagctc tttgaactgc tcaataagct acatttgtca
    23761 gggcgaaacg cagctatcga atggtactac caggaggatg acgaggacat gatggatgca
    23821 ggcaacgatt atgccgatat gctcgattta ccatttaaga tttttggcta ccaaacatta
    23881 ctgaagtaga acgggtaaaa tattatggtt gaacgttttt ttaatgagcc ttcagcagtt
    23941 acacctcttg tagaatttga ccagcagcag ggaattttca aaattcaggg aaaatcactt
    24001 ccagaggatg tgaaggcatt ttacatgcct ttgcttcagt ggtgggacga gtacagaaat
    24061 agcccaaatg ataccactaa cctggttctg gattttgact acttcaacac ggcctcatca
    24121 aagatgatac tcctcctgct cagcaagctt aaagatttat ccctacttgg caaaaacgtt
    24181 cgggttacct ggtgcttccc ttctcatgat tctgaaatgg aggaagcagg tgaggagctg
    24241 gccgaaattc ttaacattcc ttttgatttt attccaaaaa acagctagcc tggaactaat
    24301 ttaaaatgga aaagttatat atagaaccta aaaaggattc accggaggtt atctttgacc
    24361 ccaaaaatgg aaatttctcc attaccggta tttcccaccc cgagaatgtg gctacttttt
    24421 ttgagccaat attttcatgg ctcgaagctt attttgtaga attgggaagg accgattcca
    24481 tccggatgaa acccataaac gttaggttat ttttcaagta catcaactcc gctaccttca
    24541 agcagctggt tacgcttatg cagaaattta aacagtttgt tgaaacggga tacaaggtaa
    24601 acattacctg gcagtttgag cctgacgatg aggatatgaa ggaggctggt tacgagttga
    24661 tcgaaatatc aggcattaat actacctttt cctgcgaggt ttgcaacaac tagataattt
    24721 ttattcttca tgtagaacat aaagaaaaaa aggaacttag ccaaagttcc ttttactgtt
    24781 gctgttataa ttcaaattaa tctccaaaac taataccaac acccctggca gtgcgaacca
    24841 aatcgcagtt gggatcaacg tagtttggac ggttaatagc gtcaataagg ggtaccgaaa
    24901 taatgtcggg gtgacggtag gctaccattt caccaaattt tccctgaagc accatttcaa
    24961 aagctttaac cccaaactgg gttgccagta cacgatcgta tgctactggg attcctcctc
    25021 tttgaagatg tccaagcact gtttccctaa tatctgcctc aaagccaatc cttttcaaat
    25081 cgttagttag cgagtgggct actccaccaa gacgtaggtt gttgtatccc acctcatcgc
    25141 tctgctccga agttagagtg ccgcctattg gttttgcccc ttcggcaata accacaatgg
    25201 cgtgaccttt ccccttatta tagcggctgt taagcttctc tgcaacctta ttgatgtcat
    25261 agggaatctc tggaataagg caaacctctg ctcctcctgc tatggctgca tgaagggcta
    25321 tccatccggc aaagcgaccc ataacctcaa gaataagcac cctgttatga ctcgaggcag
    25381 ttgtaaccag cttatcaacc gcatctgtag caatttgaac ggcagtttga aatccgaagg
    25441 tgaaatcggt ggccgaaagg tcgttatcta tggttttggg aacaccaata atatttagcc
    25501 ccttttcata cagttgctgc gatattttct gcgaaccatc acctcctata cttattacag
    25561 catcaatacc taaatactgc agcttcctaa tcatttcatc ggaacggtca acagcacccc
    25621 aagaaccatc tttgttttta ataggccatg caaatgggcc tcccttattg gttgtaccga
    25681 taatagttcc tccttgaaca tgaatccctg atactgcctt ttcgtccaat actcttattt
    25741 ctgttggctc acgaagtatt ccatcaaaag actgaatgct tccaataacc tcccagtcgg
    25801 gttcctgaga ggctcgcttt acaaccgccc ttatcactgc atttaaccca ggacaatcgc
    25861 cgcctccagt ggctactaaa actctattca tctgctaaaa tttagttatc ttattgttat
    25921 acagaacatc tcataatctg gttcaggtta aacatggact tccatggttc cacctataag
    25981 tttcagtgct ggaaaaccaa tttttcccac ttttgcaaaa gtataaaaaa aaacaatgtt
    26041 atctttgcta tgaggcttaa tgctactgta tttattaagt acgccatgtt gcaatcgttt
    26101 gaaatttgat ggttttcctt ttcatttttt catttttttg ggaatataat gataagttat
    26161 aagtaaccct actaaaattt acatctatga gaaattctaa aaattttaca ggccatcagc
    26221 tactaatggc tattttgctt gcttttattc tggttttctc ttcgtgcaag tacagcgcca
    26281 aggaagaaat cccctttcag gtacaacatg ccgattgggt ttacaatgcc accatctatg
    26341 aggttaatgt taggcagtac actcccgagg gtacttttaa ggcctttgaa agccacatgc
    26401 ccaggctgaa agaacttggg gttgagattc tatggtttat gcctattcat cccattggcg
    26461 aggtaaaccg taaaggagcg cttggtagct actattccgt taaggactac tatggagtta
    26521 accctgagtt tggtaccctc gacgacttta agtcggttgt tgcccaagct caccagctgg
    26581 gaatgaaggt aataattgac atggtagcca accacacctc ccacgatgcc gtgctggtga
    26641 aggagcatcc cgattggtac gttcacgaca gcttggggaa ccttgtttca ccattcgact
    26701 ggacagatgt tgctaagctt gactacacca agccggaact tcgaaagtac atgattgata
    26761 tgttcaagta ctggattaag gatattgata ttgacggctt tcgctgtgat gtagcagctg
    26821 aggtcccggt tgacttttgg aatgaagctc gagcagagct caaccggatt aaacctgtat
    26881 ttatgctggc cgaagcctca aatgtggagc ttcaaaaatt tgcctttgat gccgattacg
    26941 actgggagct gcaccatacc ctgaatgcgt tagctcaagg caaaaagaat gttaacgatt
    27001 tagagcagta cttattaaaa aagtatgcta catatcccag aaacaccatc aaaatgaatt
    27061 ttattacaaa ccatgatgaa aattcatgga atggaactga atttgaaagg atgggcgatg
    27121 ccgttaaagc aatggcagcc tttacttttg ttattgatgg catgccatta atttacaccg
    27181 ggcaggaagt tggatttaac cgcaggctca aatttttcga caaggatgag gttagctgga
    27241 aggactcgct caaccttttc cctttttata aaaagttggt tgagcttaaa aaatctacgc
    27301 ctgttctagc cgccggggac aggggtgctg atatattccg aatcaaaagc aacgccgaca
    27361 gcgctgtatt cgcttttaca cggaagaatg aaagtcaagc tctatttgcc atttttaacc
    27421 tctcggctaa tcagcaaatc gcaactctta ctggcaacga ctacgctggt acctacacca
    27481 acgtaatgac aggtgaaaaa tttgttttcc aaggcgggga ggagatttca ttaaaaccat
    27541 gggaatttta tatctactgt aattaaaatt tcaaatattt gtgattcagg gcgttagtag
    27601 taacgccctt tttctttagg taaattgccg catcaacatg aacgtaatat attgtagagt
    27661 ttggtgcata tactgcaatg acccatttat cataaaccca caccgtgcta aaatcttctt
    27721 tcccttacaa cataaaataa ctttgtcctc aaatcaatag agcctccagc taaaattgct
    27781 atttttgtgc ttcagaatga tattaaactc tatttcttat gcgctattca gttcctgtaa
    27841 gcaataaaat ccattggatt ggtgttaacg accgccgaaa gaggttgttt gagaatttat
    27901 ggcctctcga aaggggtgta tcctacaact catttttaat agttgacgaa aaaacagcgc
    27961 tagttgatac aattgacggt acagccgtag gcaactatat cgaacgcatt gagcagctgc
    28021 taaacggacg ccctctcgac tatttaattg taaaccacat ggaacccgac cattcgggcg
    28081 aggtgaaagc cattatagat cgctttaagg gcgttaagct ggtagggaac gttaaaacct
    28141 ttaaaattgt tgaagcatac tggggaatta cagaaggttt gattgatgtt gaggatggta
    28201 gaactcttga tttgggatac cataagctga aattcctgat gacaccttgg gtacattggc
    28261 ccgaaaccat gatgacctac gatacaaccg atcaaatact ttttacgggt gatgcctttg
    28321 gtagttttgg cgcttttgaa ggtggaattt ttgacgatga gcttgaccct ctttactttg
    28381 aggatgaagc tcgccgctac tactccaata ttgttgggaa gtacagcaat atggttcaaa
    28441 aagccattgc caaacttgaa ggaatccctg ttagaaccat tgcaccaacc catgggcctg
    28501 tctggcgttc aaacccaacc attattatgg actggtataa taggtggagt cgctttgaag
    28561 ctgctaaagg agccgttatc atatttgcct caatgtatgg caacactgag gatattgccg
    28621 attacctggc ccgcaggctt tgtgagaatg gtgttagaaa tattagggtc ttcgatgtct
    28681 ccaaaacaca cattagctac ttgattagcg agatatggaa gtttaacggt cttgttttag
    28741 gatcgtgtgc ctacaactct caaatgtttc cactcatgga acagcttaca cacgaactgc
    28801 ttcacattgg actgaaaagc aaacatttgg ccctatttgg cagctacagc tggaatggag
    28861 gtggggttaa gaacctggct aaatttgccg gggaatcaga acttaacttg gtgtcagaac
    28921 cgctagaaat atacggaaaa cctacacctc aaaagttaaa gccttttgac acattggcag
    28981 aaacgcttgc tcacaaaatt ttggagtaac atgccttttc gcagcctttt aaaacatgag
    29041 cttctacttc atataacctt aaaactacct gataatggag tttactattc caattggagc
    29101 tgaatgcatt gtaccagagg tggttagttc tgacaatatt gcctcaaaat acggctcagg
    29161 catggttgaa gtttttgcta cccctgccat ggtagcctta atggaaaaag cagccatgat
    29221 agccgtccga ccttttctac ccgagggtta tagtacagtt ggggtggagg tatgcgtaaa
    29281 acacacaaag gctactccta ttggaatgga ggtttggagc aaagcggtat tgaaagaaat
    29341 ggatggaaag cgccttacct ttgaggtgaa ggcgtgggat gccaacggcg aaattggaac
    29401 cggaactcat atccgctata ttatagattc tagaaggttt atggaaaaaa ttgccgccag
    29461 atagcctcgc gaatattttg tagaataaac tatctcaact ctccttaaag tatggattta
    29521 aacagctata gcttgccaca gtacctcact cgaagtatgg tggtccaatc gtatgaggtt
    29581 gattttaacc agcgtttctc cattacttca atttttaacc atctgcagga gatagcctgg
    29641 gagcatgccg ataagctcag ctatggttat aatcatttaa gagaaaaggg ttggttctgg
    29701 gcgctttcaa gagtggaggt agagttttac cgcttccccg tatggcagga ggcggttacg
    29761 ctggttacct ggcctcgtgg tgccgatgga atttttgcac ttcgtgattt tgagttctat
    29821 gattcgaatg gtaataagct aatcgctgct actagcagct ggttggtggt tggtgttgaa
    29881 aaccgtcgcc ctgtgagaat tgccgaatgg tatcttgaat ctgattttgc ctcccgctcg
    29941 gctttaaatc gtaatgctgc caaaattccc gaatctatcg ttcagcctgc ctttaccgag
    30001 cagtttgaag tcagagcagg tgatatagac atgaacctgc atgttaacaa tgttcgctac
    30061 attgattggg catataacac attcaccatc agttctctaa gaaaatattt tccaaagaaa
    30121 atttcggtta actataattc agaggcaaag gagggcgatt ccatcttgat aaatcgaaaa
    30181 aatataccag gcaattccac cttgataaat atcaacagta ggctggaagg tgaaaaaaat
    30241 tactgtagaa ttcaatttga ctggagaaat ttggagtgaa tgtgaattgt tagtaaattg
    30301 acgctgttta ttaacggtaa accgaaccat tatgctcacc aagttggatt tgaagcagct
    30361 ggaggaaagg gggattactc ctcaacaggt agaagggtac atgaggatgt ttcgagaggg
    30421 atttcccttt ttgcctatcg agaggcctgc aaccgttggc gatggtatta ttagccttga
    30481 agatgatgag gtggacgatt tggttgagat gttcaaggat ttccagggca gcaaggtgaa
    30541 gtttgttcca gcctcgggag cggcaacccg tatgttcaaa catctttttg aatttcgcga
    30601 tgagtatcct gttaaaggtc tagaatgctt taacgataag ggttttaact ccgcatacag
    30661 cttctttcaa aacatcaaac gatttccctt ttacgacaag ctttataacg agctttggag
    30721 cgtaggttac cggctggaaa gcttacttga aaaaaaggat tacctgcctg tagttaacgc
    30781 cttattggat tccaacggtt taaactatgg ttctttacca aaagcgctga ttcattttca
    30841 ccgctatggc gaaattgccc gaacagcgct tgaggagcac ctggttgagg cagccaagta
    30901 cgcccgagac cagcatggag tagccaacct tcattttacc gtttcgccag agcatctaaa
    30961 agggtttcag gagctggttg aacgtgtcaa gtcattctac gaaaaccact acgaggttac
    31021 ccttaacgtg gcctactccg ttcaaaaacc gactaccgat actcttgctg ttgacatgga
    31081 gaataacccc tttcgcaatc ccgatggttc acttcacttc cgccccggtg ggcatggtgc
    31141 gctcatcgaa aatttgaatg agctgaacga ggatatcatc ttcattaaaa atatagataa
    31201 tgtaaccccc gaccatctta aagcagctac aatacgctac aaaaaggcgc ttgctggatt
    31261 acttgtcagc tataagaatt tggtgaatgg ttatatcgaa cgattgctca acgaaccggt
    31321 ttccgaggag cagctcgccc taatgcttga gttcacaacc gatgagcttt gcattttgcc
    31381 ccctgctaat ctcaataggc aaaatagcga tgctgttaaa aaatacctta tggaaattct
    31441 tgaccggcca atccgggttt gcggtatggt gaaaaaccaa ggcgagcctg gcggtggacc
    31501 gttctgggca aaaaatcctg atggaaccac ctcgctgcag attgttgaac agtcgcaggt
    31561 tgacatgggc aatccccgtg taaaggagat ttttggcagg tcaacccact tcaacccggt
    31621 tgatattgtt tgcagcataa ggaattataa aggcgaaaag tttaacctgt ctaattttct
    31681 tgatcctcaa gctggcttta tttcgattaa aagtaaaaac ggcaagcagc ttaaagcgct
    31741 tgagctgcca ggcctatgga atggagccat gagccgatgg ataaccttct ttgttgaggt
    31801 gccaattgct acctttaccc cggttaaaac ggttaacgat ttacttcgaa gtgagcatca
    31861 gcctgaataa cttgggaaag ttgctcctaa atcattttga ctgaagtttt tatttgaagg
    31921 aatgctttgg ctaatattaa tcgtttttac agaattcttc tccgtaagct cacaaaaaca
    31981 tttctttggg gtacttaact tctgtgaggt atagcccctc agcaggtgca gacgttcctg
    32041 ctaagctgcg gtcccgcgct tcaacaattg atgcaaattc ttcaggggtg atttttcccc
    32101 gcccaacatc taccagcgtt ccaacaattg cccgaaccat gttccgtaaa aacctgttgg
    32161 ctttaatact gaaaatcagc atgtcgtttt gttgatgcca gctggcatga taaattttgc
    32221 aggtgttgtt tttactatta gaacccagct tactgaagct ggtgaaatcg gaatacccaa
    32281 ctagctgctg cgttgccagc tgcatccatt ccacgtttaa atgcccatag tacatccacg
    32341 caagcccatt taagaaaggg cttttttccc tacaaatcag gtactcatac ccccgatata
    32401 ttgcatcaaa gcgtgcattc gcatcctgtc ttaccctgat tattcgtgtg atggctatat
    32461 cagcaggaag aattctattc agatggtaaa gcagagttgg gtcagcatct agtccagctg
    32521 ctgttgaatc aaaatgagcc acgtagaaac gggcatgcac accggtatcg gtacggccgg
    32581 cgccaaaggt ttcggttggg gcttccagca atgtcgaaag agcccggtta acctcaccct
    32641 gaacactgtt ggcatttgcc tgaacctgcc agccatgata gcgtgttccc ctgtacttaa
    32701 gctcgacgaa atacctgtaa atagctgatt gttccatttc aatgcgaatt tagcattatc
    32761 aattacaagg ttaaatatag cacaacttga caactcgggt taaatggctt ttgcaattct
    32821 tgtttaaggc tatgcatgga cattcagatg cgaaccggtg gttctggaca ttactacctg
    32881 taacagctca tgctagattt taaaattctt attttcaacc ggcaacccat taacttcctt
    32941 tattatatca ctcattttga tttaactttt tttaacacca ttggccttca tttattatca
    33001 tatttgcaaa cattcgtttt aattcataac cactacaata ctaattaaac tgacttatat
    33061 gagcgataca gctgcagtta acattaagga gctaaatgag cgaatccgtc tggaaagttc
    33121 atttgtagat attattaata tagaaatggg caaggtaata gttggccaga aacaccttgt
    33181 agaatctttg ctggttggcc ttttagccga tggacatatc ctgttggagg gtgttccggg
    33241 attagcaaaa acattagcca ttacaaccct gtcaaacatt atcgacgtta agtttaaccg
    33301 cattcagttt acccccgatt tacttccggc cgaccttatt ggtaccttaa tatacagccc
    33361 aaggagcgaa cagttcaacg taaaaaaagg acctattttc gccaacttca tcttagccga
    33421 tgaaattaat cgtgccccgg ccaaagttca aagtgctcta cttgaggcaa tgcaggaacg
    33481 ccaggttact ataggcgacc aaacctataa ccttgaagaa cctttcctgg ttttggctac
    33541 ccaaaatcct attgaacagg aaggaactta tcccttgccc gaggctcagg ttgatcgctt
    33601 tatgcttaag gttaacatat cctaccctaa acaggaggag gagcgaatga ttatgaggga
    33661 aaacctgctt caaacttttc ctaaacccga aagagtttta aaacctgccg atattatcaa
    33721 tgccagaaag gttgtgcgcg aggtttacat ggacgaaaaa attgagaagt acattctcga
    33781 cattgtgttc gccactcgat tccctgccga tgttaacctg cagaagtttg ctcctatgat
    33841 tagctacgga gcctcgccac gtgcaactat tagcctggct atagcttcta aggcatatgc
    33901 ttttattaaa cgtcggggtt acgttattcc cgaagacgta agagctgtgt gtcacgatgt
    33961 tctgcgccat agaattgggc ttacctacga agcggaggcc gaaagcatca cttctgagga
    34021 tattattacc gaaattctta attcggttga agtaccctag aatgagcata ttcagaattt
    34081 tgcctctggc tctactgctt gtgaatctca gcattcccat gcaggggcaa aaccttatag
    34141 gcttaaccaa gggtgaaatt ctgaagcttg tacctaccag ctgtaaggga tttaattttg
    34201 ttaaggatat ccagaatgga gataaaagtt ttataaagtt cgagaatccc accgatgagc
    34261 aaactctcct tttcatgctc aacagcaagg aaatatgcac atcggtctca agaatgtaca
    34321 atacatggct ctacgacaag gtttataaag atttgtgttg tgagtataaa caggtaagca
    34381 tggatacctg gattgacagt aaaaacggaa acgattacga ggtgaagctc aagcggggtg
    34441 agtggtttat tacagttact attcgaccgt tgataaaata ggctgtatgg aagcatctga
    34501 aatactgaaa cgggttcgaa aaattgagat taagactcgc ggtctatccc agcatatctt
    34561 tgcaggtcaa tatcatagcg catttaaggg taagggtatg gctttcagcg aagttaggga
    34621 ataccggtat ggggatgata ttcgaagcat tgactggaac gttacggcgc gctataatca
    34681 accctatgta aaagtttttg aagaggagcg ggagcttact gttatgttgc tggttgatgt
    34741 tagcggctcg cagcaatttg gaacagattt tttacttaaa cgtcaagctt tgactgaagt
    34801 agctgccgtt attgcttttt ctgcaattct taataatgat aaaattggcg ctattctttt
    34861 tagcgataaa gttgaaaagt ttattccacc ccaaaagggc agaaggcata tcctgcgaat
    34921 aatcagcgag ctgcttaact ttaaacccga gggcaccggc acgaatatta ccgaagccct
    34981 taaattcctt accaacggga tcaaaaaacg gtgtaccgcc tttctactga gcgactttct
    35041 tgatgttgaa aggctttccc taaaaccttt gtttgccgac gctcttacca tcgcaggtaa
    35101 caaacacgat ttaattggca ttcgcatatt cgataagcgc gaaagcgatt tcccttcagt
    35161 tgggttagtc aaagtgaggg acaacgaaac cggcaagtac atctgggttg actcaaacgc
    35221 tccagctgta agaagcaatt ttagcaaccg atggagtcaa ctttccgaca atcttaaatc
    35281 aacatttact aaggctagtg ttgattgggt ttctataaga actgatgagg attacgtaaa
    35341 acctttaatt tcccttttta aacatagaag ctaatgttta ggagattttg tttactggtt
    35401 gtttcttttc ttttcattta tgtaaaaagt ggtttccctc aaccgcagga gactgaacca
    35461 tccatcacct cgcggcttca aagggataca attttaattg gcgatcaaat tacctttagc
    35521 attgaggtta aaagcataaa gcccctacgg gttgtcttcc cgaacttcac cgattccctt
    35581 ggcaacgggg ttgaggtggt tggcatccca acagccgact cgctcgccaa agctaattac
    35641 caccactata gcatgaacat gctcataact gcatttgata gtggcactct cgttgtcccc
    35701 tcaatcccgg tagtgttggc atcgggccac acaaccgaca cactttttac ccagcctcat
    35761 gcgctacatg taaacctggt tcctcgcgat actacactta aggaaattta tgatatcaag
    35821 ccacccattg ctgaaccttt gacattcgcc gaggttgccc cgctggttgg ttctggttta
    35881 ctactggctg ctctagttgt tttgctggtt ttatacgtta gaagtcgtaa gcagaataaa
    35941 ccattcctga atctattcaa acctaaagaa ccccctcacc tggttgcgct cagagaactc
    36001 gaacagctgc gaaacgaaaa actttgggca aatgaaaatc ataaactttt ctacaccagg
    36061 ctagtagata ttcttcgtaa gtacatggag ggtaggtttg gtataaatgc tcccgaacaa
    36121 accacctcag aaattttaag cgaacttgaa cgaattgagt atgattttgg ccatttaaag
    36181 gaagaactgc gtgagttact ggtaatttcc gatttggtaa agtttgccaa gcatgttcct
    36241 ctaattcagg aaaatgaaaa gaatatgaac tttgcattcg attttgtgaa tagcaccaga
    36301 cccgttgaaa cgcccgaaat tcctgcaaca acggatgaca cgggacagca acttcaagat
    36361 gctcaatccg aaaatcaaac tactaataaa ttgagttgat atgtcggaaa ttgtttttgc
    36421 agcccccaag atgttttacc tgctcctttt ggtgccggtt ttggttgcat ggtacatctt
    36481 tcgtcagaaa gattccaggg ccagcctgca tatttcaact tcaataggct ttgagaatgt
    36541 aaagcccggc tttaaggttt acatgcggca tgctcccttt attcttagaa tggttgcaat
    36601 tattctgatc atcacggcat tggcaaggcc tcaatcaact aacatacaaa agaacatctc
    36661 tactgagggt attgatattg tcctttccat ggatgtctcg ggcagcatgc tggctcgtga
    36721 ttttaagccc gacaggcttg gagcggcaaa ggaaataggt ataaaattta ttagtggaag
    36781 gcctaatgac aggatcggac tggtagtttt cgccggcgag agctttaccc tttgtccgct
    36841 tactaccgac catgccaccc tgattaacat gtttcataat gttaacatgg gcattctgga
    36901 ggatggaaca gccataggct ctggtttggc tacagcagtt acgcggttga aggatagcca
    36961 tgctaaaagc agggtaatta ttttacttac cgatggggtt aacaacaggg gcgaaattgc
    37021 ccctcttaca gctgccgaaa tagccaaaag ttttggtatt agggtttaca cggttggagt
    37081 aggcaccatg ggaactgcac catacccggt gcaaacccct tttggtacac agtaccagaa
    37141 catgaaggtt gaaatagatg aggatctgtt aaccagcatc tcaaagctaa cgggaggtga
    37201 atatttccgt gccactaaca acacaaccct tgaggaggtg tatggtaaaa ttgataagct
    37261 cgaaaaatct aaaattgagg tggctgagta tagaagcagg gaggaaaaat ttcgtggatt
    37321 tttattggct gcttttgcca tactagttgt tgagtttaca ttacgcagaa ctttactgca
    37381 aggaattcag taaatgctaa ttgagccatt catgagaagt agttacaaac acataggtaa
    37441 actgttacat tgcaaaaatg tgcctttgcc ccttttgtta acaacaaatt gattgttgag
    37501 ctatgttcag gtttgcagaa ccaaaatact tttacttgct tctagtgata cctttgatgg
    37561 tgggtatata cctctactcc atacaccgtc aaaaaaaaca aattgcaatt tttggtagga
    37621 cagacacagt gatgggttta cttcccaacc tttcctttac tagggcatgg gtaaaattta
    37681 tcattttgct ggttgcttgc gtgttcgttg ttctatcgtt agcacgcccg caagtaggat
    37741 caaagctgac tgaggtaaag cgtaagggca tcgaaattat tatcgccctc gatgtttcaa
    37801 acagcatgct ggcggaggat attcaaccta acaggctgga aagagcaaag cagtcaattg
    37861 cccaacttgt tgacaagctg actaacgatc gtatagggtt gatagttttc tcaggcgatg
    37921 cctatgttca actccccgta accagcgatt tcgcctcagc taagatgttt ctttcctcta
    37981 taagtcccga gatggtatca aaacaaggca ccgccattgg gcgggcaatt gatcttgcct
    38041 ccaactcgtt cacccctcag ggtaacacca gtaaggcgct cataattatt tcggatggcg
    38101 aaaatcatga ggatgaccct gttgagtctg ccaagctcgc tgcagagaaa ggtattgtta
    38161 tatatactat tggcataggc tcgccttcgg gagcgcctat ccctattggc gcaaatcaga
    38221 actaccttaa ggacgaaaat ggaaacatcg tagtctcaag actcgatgag gaaacccttt
    38281 ccaaaattgc catcgaggga aatggtaagt acattagagc atcgaactca caatttgggc
    38341 tgctgcctct atttgaaaac ctaagcaaga tgagcaagac cgaaattaaa gaaaaaatat
    38401 actcggagta cagcgagcaa ttccaatatt tcttagcatt cgccttggca ctgctgctta
    38461 ttgattcctt gattctcgaa aggaaaaatc caaagctatc ccgttttaag ctagttgaca
    38521 ccaaaaatgg aggcaggcaa tgaaagtatt cttgatgtcc acattaatgc taacagctgt
    38581 aatttctact gcccagtcag agcgcaagtt catacgcaag ggtaaccacg attataaaaa
    38641 aaatgatttc gtggaatccg aaattcagta ccgcaacgcg ctggaacaaa actctcattc
    38701 ctttaagggc cagtttaacc tggctgatgc gctctataag cagaacaagt ttaacgaatc
    38761 gcttgagacc ctcaataatt tgaatttaac cggcttatca aacgaccaga aagcatcggt
    38821 acttcataat aagggaaacg cacttttcaa acagaataag ctaaaggaga gcattgaggc
    38881 ctataaaagt gccctgaaat ttaaccccaa cgacaaggaa accaagtata acctttcgga
    38941 ggcaataagg ttgttccagc agcaacagca gaaccagcag gataataaca acaaggataa
    39001 ggataaggat aaacaaaatc aaaaccagaa tcagccagat aacccgaagg atgaacagaa
    39061 taacaacccc gataacaggg attctcaggg gaataaccag cagcagccca aactttcaaa
    39121 agaggatgcc gaacgcattt tggaggccat tcaaaaccac gaaatgcaaa tacaggatcg
    39181 cttgaaggaa gaaaaagcaa aacaagcaaa ggttcgagtt ggaaaaaatt ggtaggctaa
    39241 ccgaagttga atatgagcaa aagatttcta ctagttgcat ttatttttgt atcaagcatc
    39301 atccaagccc agaatgtcca gtttgaggct tctgctccca atatggttga agttggtgag
    39361 caattccgct tgacatactc tttaaattca aaaccatcga attttaatcc tcctactatt
    39421 gctggctttg aagttattgc tgggccaagc acctctagca gcacaagtat tgaaataata
    39481 aacggaaagg ttacccagca gcaaagcgtt tcttacacct atattctgga agccaccgct
    39541 gtgggaaagt acaacatacc acccgctgag gcaatggttg gaaaggttac ctaccaatct
    39601 aaccctgtaa gcatagaggt aattaaagca tcatccggca gtgcgcgaca agccaatcaa
    39661 ccacaaccta ctcaacccca acaaattgcc gctggtgagg agaatttgcc cgatgatgac
    39721 ctgtttgtta ctattgagct gaatcgaaag actgcataca tgggcgagcc agttgaagcg
    39781 gccatcaaaa tatatacccg cgtcaacatt ttgggttttg aggatgctaa atttcccact
    39841 ttcgatgggt tctggagtca ggagttggaa tcttcctcca acgtgcaatt tgagcgagcc
    39901 aatgtaaacg ggaagattta taatgttggc attatacgac gatacctgct tttcccccag
    39961 aaaaatgaga agttaaaaat cgatcccttt gagctggttg tagtttatca gggaaacgca
    40021 gcaagacccc ggtcaatttt tgacgagttt tttggtggcg gctttgagaa ttaccgcaaa
    40081 cggctggtta gcaaacctgt atcggttgta gtaaagagct tacctcaagg tgcaccctcc
    40141 tcgttttatg ggggtgtggg caatttcaag attaatgtgg gtgttgataa aaataagctt
    40201 aaaaccaacg aggctgttac cctaaagctg gttgtaactg gcaccggtaa cctcaagcta
    40261 atgggaacac ctaaggttga gtttccttct acctttgaag tttttgaacc caaggtttca
    40321 gacaacatca atccatccca gcaaaacgct tcaggcagta aaacttttga atatgttgcc
    40381 atccctagag cctcgggtag ctatgagata gggccagttg attttaccta ttttgatcct
    40441 tcggccgaaa aatatgtatc agtacagtca aaaactgtaa atcttgaagt tattcccgat
    40501 tcctcggcaa caggggcagt ggtgcttaca agtggtttaa gcaaggaaga tgtgaagttt
    40561 ataggcaagg atattaggtt tattaagaca aaccctgtaa gattaaagcc tattaactcc
    40621 ttcgttgtag gttctctaat gtacctgttc tcctatattt tggttgtaat cctctttgct
    40681 gtttttctgt ttgtattcag aaaataccgc aagcaaatac aggatactgg ttttgttaga
    40741 aacaagcatg ctcagaaaat tgctcagaaa aggttgcagg tagctcaaga aatgctcagg
    40801 ttggatgaag gagaaaaatt ttacgaggaa attcacaagg ccttatgggg ttatgcatcc
    40861 gataagctta atttacccgt cgcctcgctt actaccgaaa gcgttaggga agcactttcc
    40921 ggtaaaggtg tgagtgaatc tgcaattgat gagtttatta ggattattga agcttgtgag
    40981 tatgctcgtt ttgctccatc atccgagcac cttcaattgg aaaccattta taaagatacc
    41041 tactcgctta ttttgcagct ggaggaaaat ctcggaaaag ttaagtcccg actttggtaa
    41101 aatcttaata acgtatgaag cgattactta atatagtgct gctggtttgt gctccattat
    41161 ggagtttgct tgcgcaaaat cccagccagc ttatgaagca ggcaaacgag ctttacacct
    41221 ctgggaaatt taaagaagct gctcaacgct acgaagctat cattggacag ggttacacca
    41281 gtgctgaagt ttactacaac cttggcaacg cttacttcaa aatgaaggac attcctgctg
    41341 ccattcttaa ctatgaacgg gctaaccttt taagtcctaa agacgaggat atacagttca
    41401 accttgattt agcccgtact tatgtagttg acaagattga acccctgcct gagtttatgc
    41461 tggtgacagc cgctaaaaat ttcagaagtt ggttttcctc taatacatgg gcttatattg
    41521 ccttttttgc attcctttta gtgatggtcg cggttgcgtt ttatatattt accaacaggt
    41581 acaccatcaa acgcttaact tttgccggat ctatactgct tactattgtt tttgttattt
    41641 cccttacctg cagtattagc cttcgtaatc agtccattca aagaaatcat gctattctat
    41701 ttgcccctgt tgtttcagtt aaaagttctc cagcctcaac cggtagtgat atttttatcc
    41761 ttcatgcagg aacaaaggta atggttaccc gttcgcttgg tgaatggagc gaaatcaaaa
    41821 ttgccgatgg aagcaagggt tgggtgctta gctcaacctt tgaaaggata tagcttattc
    41881 gaacattttc tgcacgcagc tgttttagta tgcagatagt actaaaactt ccaatccgat
    41941 gagaatatta gttttttgcg tgctcatgac tgttgttggc actgcagctg gacaaacagg
    42001 atcattttac gattttaccg taaaggatat tgatggtaac gattttccca tgtcaagcct
    42061 aaagggcaaa aaaatcatgg tagtcaatac tgcttctaag tgtggctata ccccacagta
    42121 tgccgatctg caagcgttat acgaaaagta tcaggatcaa aacttcgtag tgattggttt
    42181 tccagcgaat aattttatga agcaggagcc tggttcaaat aaggagataa aggagttttg
    42241 cacctcaacc tacaaagttt cctttccgat gatgggcaaa atatctgtta aggggaagga
    42301 tatgcatccc ttatatggct ggcttacaag taaaagcttg aatggagtga tggatagcga
    42361 ggtgaagtgg aattttcaga aataccttat tgacgagagg ggcatattgg taatggttat
    42421 agatcctgga gaaaaaccca attctgaaag ggtaatcaaa tggattgaag gaaaataatt
    42481 ataacaggaa ggtctgccag ttgatttaca cagaaggatg atgaattttt tacagattca
    42541 taaataggga tttacttctt tcgttgcctg tctctttcac ctttatacct accatttgcc
    42601 aaatcaactg taactaaata tcctagtttt gctacttttt ctatcagttc aggatttatt
    42661 gtttcagccc tgtcgcttgg agaatggaga tgcttgtacc cattagtagt attgaagtac
    42721 agcgtagaga tacccgccct gtaaaatgcc tctgcatcgg ctccaccccc tccaaacgtt
    42781 ctggttgaaa gtagttttgt tgtgtgcttg tctatttttt taactttttt ccacagttta
    42841 ggaaaactca accttcctcc aatggcaatg ctatcgccct gtccaacaca gtcgaagtta
    42901 agcatggcaa ccacctctga aaggtcaact ggggaatggt caacaaaata tttagaaccg
    42961 cgcaaccctg attcttcaga tgaaaaggca ataaatatta tggagcgttt aatttccacc
    43021 ttcgattttt ttaaagcctc ggcaattgca attatggctg ctactcctga agcgttatca
    43081 tttgcacccg gaaagtagag cttttcacct tggtacccca catgatcgag atgtgctccc
    43141 agcacaacgt actcgttttt aagcagcgaa tcgcttccct caacaattcc aactacattg
    43201 gcagtgggtt tttcagcaaa gtattcagct ttaacattta ctaaaacgct tctacccagc
    43261 tcaattgagt gcggagcttt atcttgacaa atttgctcat acaagttagg ggcttttact
    43321 ggcagctcgg caaacaagct gtccgttgct ctatcgctaa gttgaatcat tggaaaatca
    43381 aacaggtggg ggccatcgcc acatgctatg cttccatata tcatggtagg cggaaagacc
    43441 tttggatcgg ctataaaaat gattcccgct gctccttgtt ctttagcagt tctcgctttt
    43501 gctctcgggg aggtgtcgcc ccagctgctc gtttctgacg tccaaggagg agccgatttg
    43561 aatgctacca ctatcttgcc tttgacgttt atgttgcggt aatcatcgta gttgggttgc
    43621 ttcatgccaa atccggcaaa aacagcttcg cccttcacat gacccgatcc tgtaaaaccc
    43681 cggcacacaa agtcttcacc aagagtaagc gggataatgg ggcgtccatt actatttagg
    43741 ataaagcagc tagcgtcatg tatgaggttt acctcatcat taaagtactg gagcatgctt
    43801 ggctgtccaa ccggtttgat tcctgcattt ttaaaccggg ttgcaacata tctcgcagcc
    43861 tgctcgtact ccatgctccc gggtaacctg ccatggaacc gcgatgagga aaggaagcca
    43921 accgtttgaa tcagctcttc ctgttttata atgccagctc caaaattctc caaattttgc
    43981 gaaaaggctg tttcgccagg ttgtagcaaa acaaccatgc aaatcgctat tttaaatcct
    44041 gcattcatcg aataactcct taaaatcttg atttcctttt caaaatatct tcattttaag
    44101 tacctaaacc gcattttcaa aggtagatat aaaaatagga gtataataca cgtgcatata
    44161 aaaactgttg gttttaacag ctacttttgt actaaaacat tctatcaata tgacagattt
    44221 tctcgactca aacctgtatt catacctggt actcccattt ctgattttct gtgcaagaat
    44281 ttgcgacgtt actattggca cacttagaat tatatttgtc tccaagggac ataaaaaagt
    44341 cgctcccatt cttggctttt ttgaggtatt tatatgggtg attgccataa gccagataat
    44401 gagtaacctt aacaactttg cctgctactt tggatatgcg gcaggattcg ctacaggcaa
    44461 ctacattggg atgctggttg aggaaagaat tgccgttgga aagcttttca ttagaatcat
    44521 tacttctgct gatggaacaa ccctggttaa gcttctgaat tcaaatggct ttggagctac
    44581 tacagtaaag gcagaaggtg gcaggggagg ggtgaatatg gtttactcgg ttatcaacag
    44641 gaagcggctc gatgaaattc agaaaatcct tctggacttc gatcccaagc ttttctatac
    44701 aatagaagat gtccgaaagg caaatgccgg aatatttccc gagtcttttt catccaaaat
    44761 tcctttattt tcccgctgga gaagagggaa gtagtttcta gagcaaaagg attcccccat
    44821 tcctgctgat tgtatactta ttaacggacg tgatgtggaa tgctggcggg cagctcaatg
    44881 gaaatttcca ctaatcctcc aagttcctct ccatcaaacc aaggcgtctg gtagattagt
    44941 ttttttactc cgtttttctc aatagtatag caattacttt ccttgttttc tattaaacgt
    45001 ttaatggttt ccaccgactt ttcgctatga cacccccaaa ggcgttgccc aataagttcc
    45061 tttccacccc acttttcaaa aactttttgc gatttctcat tcatgtagag aatggttcca
    45121 tcggctgcgc ttacagttat tgcacaacct aacccaatcg cccagtctat gccattgatc
    45181 ataaccttga tttttgggca aaataagtta ttacttttca tccagcgcaa attacccacc
    45241 aattttttac cttgcagcat aaataaatta ttcctgcaat gaaaacatac aatgtaaaat
    45301 cttaccgtac acctctttat agagatgccg gctttctttt gaaggatatt ccaacagctc
    45361 aaaaagcctt ttctgatgaa caatccctcc caattacccc cgaactgtac atttactccc
    45421 gctataggaa tcctactgtt gttgctgccg aggagtattt ggcttctatc gaaggctccc
    45481 agtggtcact tttatgccag tcgggaatgg ctgcaatcga tattgcccta tcggttttcc
    45541 aaagggcaaa tcataaatgc aaatgggcat ttttcaccga aatttatggt ggtacaaatt
    45601 catatattga tagcatcctg gtttccagaa ggggtgttga cgcagtacgt tttagtccta
    45661 gtggcgaagc gtcttcaaat gtagaattcg attcgtttat gaccactcat aaacctgatg
    45721 tggtattttt tgaggtcatt tccaacccta tgcttatcgt tgccgatgcg gttgagatta
    45781 ttcgcatagc aaagaaatat ggagctgcag ttgttgttga taatactttt gcaaccccat
    45841 acctcttcaa accactcgac tttggtgccg atctggtaat tcatagcgct acaaaatacc
    45901 tttctggtca tggtaacctt tctgcaggcg tagtttccgg aaatagcccg gagctagcca
    45961 ggtcagcgat tgaatatcgt aagctggttg gccacatgct ttctccagat gatgcctacc
    46021 gccttcatga tatgctgaaa accttcaacc ttcggattaa tcgccatttt gagaatgcgg
    46081 ctaccgttgc caagtttctt tctgcaagca gcaaggttga gaaggttata ttcccaggaa
    46141 tggattatca cccttcgcac gaggttgcct ccaaactttt tgagggtagg ggatttggtg
    46201 gtatagttac cttcgatctt aagggcgctt cgtttgaaga taagcagaaa aaatgcaatg
    46261 agtttattaa tgccgtttcg gatagcatac ccctaatccc caccttgggg gatgctgata
    46321 ctatcctcat gcctgttgag cctgtttggg gcgataaata cccattcccc ggtatgatta
    46381 ggttatcgtt gggcattgaa aacggtcaag aaatcacccg ggtgatttcg caagggctcg
    46441 atgcagttgg aagttgattc ccctggtatg ggctaatgac caaccttttg gtgaggttac
    46501 atcaaccttt gtcaggataa ttttttaaga ctgaccaccc tgtttgtgta ttagcttcct
    46561 tttgtgtttt gggctttata ccagaagaag tagctattct gcttgagctt ttcctttcgg
    46621 cttcgggcca cataaatttt ttcaccagta tagggattta ttcctgtgta gtacattgcc
    46681 gaagctaggg tcattggcgt tggagtgaaa tcttgcactt gctcgggtct tatatttagc
    46741 tccttaatct ccctggctaa ctcctgcatg tctgcttcgg tacttcccgg atggctcgaa
    46801 ataaagtagg gtataagctg ttgatttaaa gcatattttt tatttataag gtcaaattcg
    46861 gtcttaaatt gccgaaactt cctgaagctg ggttttctca tcagtttaag aactttttcg
    46921 gacgtgtgct ccggggctac ctttaaccta cccgagatgt gaaaacgaca aaggttttcg
    46981 aaataatcct tttcggcctg ggtgtttctg cctgtaaaca aatcatacct taccccgctg
    47041 ctaacaaacg ccttcttaac cccatgcatg ctgcgaattt taatgtacag gttgtttgct
    47101 gcgctatggc tggcatttag gtttaagcaa atggaaggat agatgcagga gtaacgagtg
    47161 cactccctgc atttttctcg ctttattcca cccatcatgt acatatttgc cgaaggccct
    47221 ccaatatcgc taaggtatcc cttaaagcca ggcatttcaa ttacctgctc tacctctcgt
    47281 agtacagatt tttccgacct tgaagcaatg aatttacctt gatgagctga tatggtgcaa
    47341 aagctgcaac ccccaaagca cccgcgatga atgttgaccg aaaattttat catttcccat
    47401 gccggaatat cttttccctt atacctggga tgtggtaatc gggtgtaggg taatgcatac
    47461 cacttgtcta tttcacctac agcaggcggt ggatagggtg ggtttaccac aacaaaacca
    47521 tcaccaacag gctctactat cgttttctga acaagcttgt tggattccct ttcaaaaagt
    47581 ttaaaatttt cggcaaaata ttttttactc tttatgcaat cttcatagga atgtagttcg
    47641 atgaggctac tatctgcagc aacttctgga atattagcga cgaaaaaagc ggtttggggt
    47701 atattcttta gttcggatgg tgctttacca ttagctatcc gtcgggctat ttccacaatt
    47761 ggctgctcac ccattccgta cactagtata tcggcatgac tatcaatcaa aatgctgggt
    47821 ttcagcgtgt ccgaccagta atcgtagtga gtcagtcgcc ttaaagatgc ttcaattccc
    47881 ccaatcacga taggcacttc agggtagagt ttcttaagta tgttgcagta ggttgttatg
    47941 gcgtagtcgg ggcgaaaccc tgctttccct cctgctgagt atgcatctgt gctcctaagc
    48001 ctcaggttgg ccgtatagtg gttaaccatc gaatccatat tgcccgaggt aacggcaaaa
    48061 aaaagttttg gcctgccaag cttgcggaaa tcccttaaat cgtctctcca gtttggctgt
    48121 ggcacaatgg ccaccttgta tccctcgttc tccagcaccc gcccaataac cgctgcccca
    48181 aaagcaggat ggtcaacgta ggcatctcct gtaaataaaa ttatatcagc gctctcccaa
    48241 cccagagcat ttatctcctt taggcttgta ggtagaaata cgctgttcat catattttat
    48301 gacctgaaaa gtaagctgga gtctccatag ctaagaaaac gaaaatcatt cctgagcgca
    48361 taggcataaa catcacgcca ttgctcacca ataaatgccg ataccagcag tagaagagtc
    48421 gaacggggct ggtgaaagtt ggtaacaagc gcgtctgccg tttgaacgtt atatcctggt
    48481 gctataatta tttgtgtcga ggccatcagg gtctctaggc cattagcctc catgtggcgc
    48541 caaagttcat aaaaggctgc tttgaaggga ataggttcag ttttataggg ttcccattgc
    48601 gaaatgaagt atggagggca tagctggttt tgggaggcct ttacgccaag ccaataaaga
    48661 ctttcaagtg ttctaagggt tgttgttccc accaccactc taaaattgtc gcattcggac
    48721 atacccttta tgaggtcaat atcggcaagg aaatgctcgg tgtgcatttc gtggttgatt
    48781 attacatcgg ccttaactgg cttaaatgtc cctgcaccaa cgtgaagcgt tatgtaatca
    48841 ggagtaattc ctaacttttt taaattgcta aatacctcgg gagtgaagtg caaccctgct
    48901 gttggtgctg ccactgaccc ttcgggttgg gcatatatgg tttgataccg gtacttgtca
    48961 acttcttccg attgcctatt caggtagggg ggtatgggca cttcaccaca aagctctaca
    49021 acttgtgcaa agcataggtc ggtgttggtc cagctgaact caacctcaaa tccatcggac
    49081 acctttttcg attttctcac ctttaattta gtgtcagttt gaggtactgt tcgctctagc
    49141 gtatctgttt tccatcgctt ttgatttcca accatgcatt tccatactac tggttcgtgg
    49201 cttgaaaagg aaaccgaata atctgccggg aatacaggtt ctagtaagaa aacctcaatt
    49261 gtagccccgg tttctttcag gaagattatc cgggcactaa ctaccttggt gttatttaaa
    49321 actaaacgac tcccttcagg aaggtagttg cctatatttt tgaaaacatc gcttccaatt
    49381 tccccatccc tatatatcaa caacttcgac tcatcccttt tacttaatgg gtggctggct
    49441 attcggtcgg ttggtaagct atactcaaaa tcggaaatgc taatattgcc tggagtaaac
    49501 atcacattag aattcaaact cacgctgttg tatccctatt cccttgaaat ggcaaaatta
    49561 gctaattttg cagttctttt aactttaatg gattgggtta aatcatgagt ttaaaaataa
    49621 atagagtatg aaagttgagg ttggagatag ggtgcgtttt ctgaatgagg ttggcggggg
    49681 taaggttcta aggattcttg ataagcgaat ggttttggtt gaaactgatg acgggtttga
    49741 aataccggtt ctggcagcag aagttgttgt tattaaccgg aatgccgaca gtcagattat
    49801 tggtgatgat tcattaccaa tgcccactct tcccaaacga acagataagc atcctaaaga
    49861 aagcaatcaa aaggcaggta gtgcagcagg cataagcatg caagtagcaa cggagagaca
    49921 gggtgatgat ataatgcttt tacttgggtt tgttcctgat agcctataca cgcttgcaag
    49981 cagtggctta aagttgtttc taattaacga cagcagctat aagatttttt acaccttatc
    50041 atcatggaat gaaggaaagc taaatccaat tatttcggga ttgcttgaag ccgatactaa
    50101 agaacaggtt gcataccttg taaacgatga ggctagaagc gcgaaaacgt tgaacttgca
    50161 ggctatttac cacaaaggaa tgccatttaa agcctatcag ccagaattct acgatgttga
    50221 gatagcctcg ctcaagcttc aacttgcagc cagctacgtg gaaaatgatt atttcgatga
    50281 taaagcctgt cttgttacta ttgcagatac ccaaaaagag gtgctgttgg aaagaatgga
    50341 cagggaatct atacagggaa tcatacaaga gaaggaaaac cagcacgtta agaataagcc
    50401 taaaaaatca cttccggtca atgaaattga agaggttgat ttgcatattg aagagctggt
    50461 tgatagctgg agtaaccttt ctccaggaga aattcttaat attcagcttg cccgctttga
    50521 aacagcgctt cagggaggca ttaagtccaa caccaaaaga atggtgttca ttcacggcgt
    50581 tggtaacggc aagctgaagt acgaaattca caagctactt gacaccaggt ttgctggttt
    50641 aaaatatcag gatgcctcat ttaaagagta tggatttggt gcaaccttgg tttacctaaa
    50701 gtagtataat cttctatagc aagttatccg ctctatcgct tcttgaaata caggagagga
    50761 aagtccgggc agcaaagggt gccatgcttc ctaacaggaa gatgctcgtg agggcatagt
    50821 agtgtaacag aaaataaccg ctctgctgct ggcagggtaa gggtgaaaag gtggggtaag
    50881 agcccaccgg gtttcgtagc aatacggaat gccgtacacc tcatgggctg caagaccatg
    50941 tatattccga tctcgtaaga gacagggtta cccgccttgc tccactcata ggtggtatcg
    51001 gaaggggtag gtcgctttag gctgtaggca actacagtca cagatgaatg gtagaggcga
    51061 ttaacatcgt acagaacccg gcttacagga taacttgcta tttttttact ccccaacagt
    51121 tttaattatg ttaacctgat attggcctgt atggtgttat ataaatttat aaggttacag
    51181 atgtaacttt aatataatta aaacttttta aataggacag ttatttttga tctttaagca
    51241 taactaaata ttaaataata ttctaataaa caacatttta gataatactt aaaattaaag
    51301 tgattacaca tataaacttt aatttttcgt tttgattttg ttctacgctg attaaattac
    51361 gtgcggcaac atctatcatt cgtaacaacg ccagaaagct cattttacag gacatacaag
    51421 ttgatatatc aacgctattt tatttggttt tataataaac tgataatctt atgtttatta
    51481 atatatgtta atctaatatt ttataaagaa ataaattgtg gctgtatttt gaacagtaaa
    51541 atagttaaaa taacattata aatacctact atccagatat attgttctat ttaaataatg
    51601 tttaaataga actgtaggag taggtagcct aaagtgctat aaaatatccc gattcaagca
    51661 ggttaagcgt ggctaaaata agcgtagctt catttttagg cataaagaat agagtttacc
    51721 ccgctactat gggaactaac tatttttaaa aacttcagcc ctaaattttt attggttaca
    51781 tatgtaattg ttttaaaatt ttgatttgcc ggttataaaa tttacatttg tatgctcaac
    51841 gttaaaatca gatcgctaaa tggagatgaa ggatagaata cggttattgc tggatgcaga
    51901 aaaaattaac tcggctaaat ttgccgaagc aatagaagtg caaagaagcg ctgtttcaca
    51961 catactagca ggcagaaaca accccagctt tgatattatc cagcgaatcg cccaaaagtt
    52021 ccccaaaatt aatcttgagt ggttgattac cggaaaggga agcatgtaca aaatccccgt
    52081 tcaacgatca atttttgata cggacgattc aattaatcaa ccaggtaaaa cagcccctac
    52141 ggcaaatttg actgaaagtc gaacgattac aaatgtaacc aacaggggtg agggtggaaa
    52201 aattttatcc ggtcgatatg aaggtgccga ttcggaggat acctcaaaag aacttacggt
    52261 gcaaaaaatt gttatatttt attctgaccg cacgtttgtg gagtataggc catcaatatc
    52321 ctgaattttc tctttcaatt ttcttacttt tgtagaaaat ttggtcatgt acaaatccat
    52381 ttttaggaag ttgctattta tttttccacc cgaaaccgtt cacaagctgg ttgtttccct
    52441 cataaaattt tttttcaaaa ttcctggagc aagaaatgct tttaggagaa ttttttccaa
    52501 tagaaatgag gcatgtttag aaagagaagt aataggctta aaattttcta acccggtagg
    52561 acttgctgct ggattcgata aaaatgccga attttttaac gaattttctt catttggatt
    52621 ttcgtttatc gaaatcggca cggttacgcc agaaccccaa cccgggaaca gcagacctcg
    52681 cctttttaga cttttacccg ataatgcgtt ggttaaccga atggggctaa acaataaggg
    52741 agttaactat gttaagtcga gattgcagca caaggaaaac caaggtttaa ttattggtgg
    52801 aaatattgga aagaactcat ctacgcctaa tagtaatgcc gtttccgact acattgcatg
    52861 ctttaatgag ctttactcat ttgttgacta tgttgccgtt aaccttagct gcccgaatgt
    52921 tcataacctc agcgacctac aaaatcccga tagcgtgaag ctaattattg aatcgctggt
    52981 taatgaaagg gcaaaatttc cttcgaaaaa gcctattctc ctcaaggtct cgcccgactt
    53041 gagccctgaa cagcttaacc aaacaattga tgcagctgaa gaggctggtg tggatggcta
    53101 tatcgccacc aataccacta ctggacgtaa caaccttaaa actaaaaaaa gcgaactcga
    53161 gaagattggg agaggaggac ttagcggcaa gccgctccgc gatgtttcaa ctgaggttat
    53221 ccgcttcata aaccaaaaac ttgacgggaa aaagccgata attggcgttg ggggtatttt
    53281 caccccaatg gatgcgttgg aaaaacttga agctggagca tcgctggttc aagtatacac
    53341 aggtttcata tatgagggtc catttattgt taagagaata aataagctat tgataaataa
    53401 aatgtttaac acatatatat aaatagaaat ggctaaactt gttgattgca ggggaatggt
    53461 ctgcccccaa cccctggttg aaaccaggcg ggaaatcaag aaatctaatc ctggtgaggc
    53521 cataacggtg gtggtggata atagcacttc acttaaaaat atcctccgtt ttttagagga
    53581 caatggggtt gaatgcgttg tgaagcaaga agcagaaagc agctcaatta cctttaatgt
    53641 tcctctacaa atgactcaac cggataagag agcagaggaa tactgcgatg tttctcccaa
    53701 acctgatgtc aattccggtt ttatcgtact gctgaatagc gccacaatgg gtaagggaaa
    53761 cgacgagttg ggtaaaattc ttatgaaagg gtttctcagc actcttctgg agcttaaagc
    53821 tacaccaacc gaaataatct gttataatag tgcggtaacc cttgccctta aaagttccga
    53881 cactgctgca actcttcaac aaatttctga aaggggtata aaaattactc tttgtggaac
    53941 ctgcgttgac ttctacaact taagggacga tttggccgtg ggccaaattt ccaatatgct
    54001 ctatattgcc gaaaggctgt catcgggtgt gaggataatt caaccctaat attattcata
    54061 tcatttttat ttctagttga ttaccttatt aaactgaaag tagttaatta ggtagattaa
    54121 gtaataaaat gaacctgtac ttcgataact catcaacaag cttccctaaa cccgaagggg
    54181 ttagccaatc catcaacgaa tactttagcc atggtggtac ctatggaaga gcagcctaca
    54241 atcgtgttat cgaggtttcc cgtaaggttg aatctacccg tagcctattg gccaacctta
    54301 ttggaaccag ccttgccccc aatgtggttt ttacttcaaa tgccactcat gctattaact
    54361 tgtttatcag aggaatgcga taccccaaga ggagggtaat tatttctcca cttgagcata
    54421 acgccgtttg caggcctcta caatacttat tccagactgc aggggtcgga gttgatactt
    54481 tccctcatca tcccgatggg agaattgata ttcaaaaagc cgctaaggtt aactttgcaa
    54541 gctacgattt gggaattatc aaccatgtaa gcaacgtgaa tggggtaatt cagcccataa
    54601 aggagataaa aaggttaatg ggcaacaccc cactcctggt tgacgccagc cagtcggccg
    54661 ggagggtcga gataagagct gacgaatgga agctagatgc tttagctctt accggccata
    54721 agggtatcat ggggccaact ggaaccggtg ccctattcat ccgcaatccc gacatggccg
    54781 atcctttaat tttcggtgga acaggatcaa attccgagag ctacctgcaa cccatcgacc
    54841 tacccgatag gtttgaagcc ggaacaccta atatcgtcgg aatctacggt ttatggggag
    54901 ctttatcatc gggtatgggc tactctttta aacgcgagca cttcgtagag ctcctgcacg
    54961 aaatttctac attttcttcc tttaaacttt tggcggctga cgacacggaa ttccaatcgg
    55021 acgtcttctc cattaaaccc cttggaatgg aggtgtcaga acttgctcgc attctctttg
    55081 aaaaattctc ggttgaaacc cgaagtgggc tacactgtgc acctcttgcc catcgcacgt
    55141 tgggaacttt tcctaccgga gccgtacggt tttccctgtc aaagtttcat actgataacg
    55201 acctcgacta cttgcatagc tgcttaaaaa aaattgatga gggaagaagc taacacagga
    55261 tttacaattc tcatattcag caacatccac caggtaatgc atgccgaaaa gattattgct
    55321 gacaagggtt atccgtacag aatagtaccc gttccatcaa gccttagctc ggaatgtggt
    55381 ttatgtataa gaattgaaag ttcatatttg gatagcattc tatcagattt acagaatatt
    55441 aaggtcgaga ttcataccct aaataatgaa ccttaaccac ctttaaaaag aatataaatt
    55501 acaagatgat ggaagaattt gacttactat caaccgtgga gcagggggga tgttcctcca
    55561 agattcctgc tgcattgctc gaatcccttc ttcccgcatt gccaaagctt gttgatgaaa
    55621 acattctggt tgattttagc tcccatgatg atgccggggt ttaccggatt tcagacgatt
    55681 ttgcgctgat tctaactacc gattttttcc ctccggtttg ttccgatcct tttgagtttg
    55741 gccaaatatc cgcagcaaat tctattagcg atgtatacgc gatgggggga gagccattcc
    55801 tcgcccttaa catttctatg ttcccctctt ccaggatacc cattgatgtt tacaagtcta
    55861 tacttgaagg ggccgcttca aaagcgctgg aggcaaatgt tgcaatcatc ggaggtcata
    55921 ccattgatga tttcccccca aaatttggct tggctgttgt tggccgtgtt gatcctaagc
    55981 ggttggttac caactccggg cttcgccctg gtgataagct gattctcacc aagcccctgg
    56041 gaaccggcat taccgtagcg gcaaaacggc ttaaaatgat tacagacgaa tcgtaccgaa
    56101 ttgccattca atggatgaaa acattaaacg acagagcctg cagcgttatg aaaaaatatc
    56161 atgttaccgg ggcaaccgat attaccggct ttgggctttt aggtcatttg cttcgaatgg
    56221 ccaggtccag cagagtaaac atttcaatcc aagccggcac gcttccggtt ctgcccggag
    56281 ttaccagcct ggtgaacgat ggctgcattc ccggtgcagc cttccgcaac ctggagtatg
    56341 tagaaaacga tgtaaccttc acccgcgttg actacgactt aaaaatgata atggttgatg
    56401 ctcaaacatc aggaggcctg ctgatgggag ttcaggacga atttgccaat caaacccttc
    56461 aggagctgac tgatgctgga ctttgctact ccagaattat tggcgaggtg ggagagggga
    56521 atggtaaaat tttcgtttct tgatggttat tggtaatcat aacaaacata cattttataa
    56581 taaatatttg aatatcaata taatatgaat ttttttcgaa gtagaaatgg atattgtagc
    56641 agcaggtagt aaaatttgaa tttacagtgc aatgttgtca tgcaataatg ctggaataag
    56701 aggtcattag gtcgtttact aaatcaaatt ttatgaagct tacaaaggat tcctggatta
    56761 cgtttacaat cgaacgactc gttgaaattc ctcaggcagg atattactac cgtttgcttc
    56821 atgaatcggg acaaaaattt cttctgcctg cccagtacta cactcattat aaccttgtag
    56881 ttggaagcac agtcagctgc cgggttgata agattaactg catggggaaa gtattccttg
    56941 aaccaaaaca tccttactat gaagttaaca tggtttacaa cttcgatttt gtagccatga
    57001 ataattcttt tgttaagggt gttgccgagc tggttgtaag ggacattttt aacgagacct
    57061 acacgttatt ccttcctgct gaggtctgct caaaaatatc aaacatggtt cctcttaggg
    57121 tactcaacat aaggaagggg aggttacagc tgcagcttgc aggagcaaca ggtagctgca
    57181 aggaatttga ggaaaacgaa atcattgacc taactattga gacggttgtt gaaattaatg
    57241 gagaaaaatt cttttttcta agccatagca gcgggtgttt tgctttatta aaagtaaagt
    57301 actaccttca ttacaatttt gaagttggaa agcaagttag ctgccagtat attgggataa
    57361 aaccagacgg aaccttaaga gttgagcctc caaacccttt ctacagggta ggagatacct
    57421 ataactttac ggttagcagt gtaggtacag aaagcaattc tgatggtgaa acagctagct
    57481 atgcaacagt tttcgatata tatgggatga agtgtggttt tgttgttcct gcaggcagta
    57541 aggtgcaacc cgatcaagtc atactatgta aagttgtagg gtacaaaaaa ggacgaccca
    57601 tgcttatgat cgtccaaaaa tgagagcata taccagttaa gtcaacctat aaatcatcgc
    57661 tcatttcctc ggatggctca tcctcttcat tcatatcgtc cggaaaggca tcgttttcgc
    57721 taccaatgtc atcgcttaag cctccaaaaa attccttttc gtcttcctca tcctttattt
    57781 tagagtcgat tttaacattt acttttacca ggtagctagc atcatcggaa tcgagggtaa
    57841 cagcaaaaaa ataatcacct cccggctttt ccaccttaat tacatgattc tgatatccat
    57901 gtggatattt ttgcttaaaa agttccaata gctccggtga aagatcacca aatttgataa
    57961 caactctttt cttagtcata ccctgaaaaa aatttttgca attatacacc aaaattaaat
    58021 aatgcaaacg agccctgtaa aaataatttc attttttaat agaagcacaa tcagtaaacg
    58081 attactacct ttgggtagaa ttttatagca atgctttaat gcattttttc tcaacattaa
    58141 ctatttaaca tatcgtgatg ataaacaaat tattgctagt tgtgacttgc gttgttccta
    58201 tactgtttgg gtacaacgct atggctcagg ttcaaattta caatcccaat gctgatgcca
    58261 tcaaggattt aaatcaggct attgaaaggg ctggcgttca gggtaagcat gttctggtgc
    58321 aggtaggtgg aaattggtgc ccttggtgtg tgaagctgaa taagattttc acgacagatt
    58381 caaccattat gaaggtgttg aacgcaaatt acgtgctggt taaggttaac tacagcaagg
    58441 aaaataagaa cgaagcggtt cttgaaaagc tggaatttcc tcagcgtttt ggatttcccg
    58501 tgctggtaat acttgacgat catggcaaga gaattcacac tcaagattcc ggattgctgg
    58561 aatccggaga tggttacgat acaaaaaaga tagtaagctt tcttaaaaac tggacaaaag
    58621 cggccatcgg aaaaccggca aattagtcct ataattaata ttatgttaaa tataggtatt
    58681 taaaaaagac cccgacttta tcggggtctt ttatactaca tattgtttag cttgtcgata
    58741 atctccttat actttgcact catttccgga ctttcatttc taaagcggaa gtaaatgttc
    58801 tttagtgcat ccataacctc cttgctggcg gggttgattt tcagagcgtt ttccatataa
    58861 ggaagagatt ttttgaattc aacattagcc tttgacataa gttcatcata cttttcaatc
    58921 tccttagctg gaatcttatt ggcttcttct acatacttta cacctacatt gaagaaaagc
    58981 gctccaatgt tgtagtaggc atcaaagtag ttggggtcaa tttctattgc cttttggtat
    59041 gaagttaagg cttcgtcagt atcgccaagc ctatcatgca gggtcccttt ggcaaagaaa
    59101 agtgaagggt tattaggttc gcttgcaata gcttggtcta tgtaaaccaa aattttcgaa
    59161 gggtcttccc ccttatcaat gtagtagcta ataagtgaaa aaagaacatt ggtgttttta
    59221 ggatatttaa taaatccatc ttccaggtat ttagcgcctt cgtcagcctt acctagtgat
    59281 ttgtatgcgt caaaaatgtt gaagtaaacg ctaccctccg aggtgaaacc cagcttaata
    59341 gactcttcat agtagggaat gcattcttca aatttcccat cgagctgtgc agataaccca
    59401 gcatagtagt atattgcggt gtctttctga ttaactaagg ggtttttgcc aatttcaaca
    59461 accttactaa agcatttata tgagcagcca taatcttttt tgctgtagca gcttgaccct
    59521 tcgttgatta agtagttctt caattttagc aagtcttcca ttattggctt ggcctttttc
    59581 ttgcttgcat caagctcaat ggctttgatc aagctttcgt atgctacctc caaaggattc
    59641 tcaacaacag gtttagttac ctgccagtga tctaatgagc cgttagcaaa gtaaaattca
    59701 gctctatcca tcacgaactt atccacggta atgccttcaa caacttcctg agtttgctca
    59761 gttggtttac caattagtaa tgaaaatgtt ttcatgtcca ttccagggta tgcccggtaa
    59821 atctgagcat caaatacatc cataaacaac tctgccctag caatccaagc atcgggtttc
    59881 gcattttttt tgggatcctg aatggtggcg ttacttttct caattttatt accaaactga
    59941 tcaaagttaa tttgtacgcc agtttgtgcg ttgagcattc ccatacccag cacagaaaat
    60001 agtagtacag ttgttaatcg tttcatttca tgtaaattag ttaaagttaa aaattaaatt
    60061 caaaaataaa ttatgtgttt gggtttttca ttgttcgctt tctccttctt gaactaaatt
    60121 ttgctcatct ggttcatttt cagatgattc aacattggct attgcagcga tactatcatt
    60181 cttttttagg tttatcagcc taactccctg ggcatttcga cccataaccc taagttcgct
    60241 gattttaagc cttatggtaa gcccgctgcg attaataatc attaagtcgt tattttcatc
    60301 aaccgcttta atgctaataa gcttccctgt tttaggggtg atgttaatgg ttttcacccc
    60361 tttcccaccc ctgttggtta ttctatagtc ctccaacctg gatcgcttgc cgtaaccatt
    60421 ttcagaaaca acaaggatat cttttgatgc attttcctca acacaaacca tgccaaccac
    60481 ttcgtcgtca ggggaaatag aaattgccct aaccccacta ccagttctac ctattgggcg
    60541 gacaagatgt tcggggaatc gtatggcctt tccatttctt gcagcaatca gtatgtggct
    60601 tttgccatct gtgaggcaag cctcaataag ctggtcgccc tccttaacgg ttaccgcaat
    60661 aacaccgttt tgacgtggcc ggctgtaagc ctcaagaggc gtttttttaa taatgcctct
    60721 tttggtacac agcacgatgt agttgttttt gagataatct gtgtcatcaa gccctttgac
    60781 attgatgtag gctctaacta catcgtcatt ttcaatgttt atgagattct gaatagctct
    60841 tccttttgat gatttagtac cctcaggaat ttcataaact tttagccaga aacatttgcc
    60901 gttttcggta aaaaacagca tggtattgtg catagtagca acatacaggt gctcaataaa
    60961 atcctcatca cgtgtatcgc tacccttagc ccctacacca cccctcgatt ggagcttata
    61021 ctcataaagc ggggttcgtt taatgtagcc catgtgcgag atggttatga caacttcctc
    61081 atcggcataa aaatcttcag ggttaaactc ctcggcgtct ggaattatct ctgtttttcg
    61141 ggcatctcca tacttttgct ttacctcaac cagctcatcc ttaataatct tcatttgcag
    61201 ggctttatcc tcaagcacct gtttaagata ggctatcagc ttttggagct cattgtactc
    61261 ctcctttact ttctcacgtt caagtcctgt aaggctgcgg agccgcatat caataatagc
    61321 ctgggactga atatctgaaa ggccaaatcg ttcaactagt ttttccttcg cttcggccgg
    61381 tgattgggct gaacggataa ggttaataac ctcgtcaata tgatcaatgg ctataagcag
    61441 gccttcaagt atgtgggctc ttttttcggc ctcctccagt tcgaaccttg acctgcgaat
    61501 aattacgtca tgtcggtgct tgataaagtg aacaattagg tccttaaggt tcaacagctt
    61561 cggccttcca tcaaccaggg caatgttgtt gatggcaaat gatgactgca gctgtgaatg
    61621 cttaaaaagg ttgtttacta cgacattagc aaccgcttcc tttttaagaa ttattacaac
    61681 cctcatccca tccctatccg actcatcgtt gatgtagcta ataccatcaa tttttttctc
    61741 gtttataagg tcggcaatcc gacggatcat ctcggcctta tttaccatgt agggtatttc
    61801 ggttacaatt attttggacc ttcccgactc ggtttgctca atttctattt ttgaacgaac
    61861 cacaatttta cccttgccgg tccggtaggc ttccttaacg ccctgcgtgc cataaataac
    61921 ccctccggtt gggaaatcag gacctttaac atactttaac agctcatcag tagttatatc
    61981 aggactatct attgtagcac aaatagcgtc aacaacttcg cccaggttat gaggaggcat
    62041 gttagtagcc attccaacgg caatacccga tgtgccgtta accagcaaca agggaatttt
    62101 agttggtaaa acagatggct cctcaagggt atcgtcaaag ttaaggttga aatctacagt
    62161 ttgtttatcc aggtcagcta gaacatcctc tgcaatttta gccaggcggg cttcagtgta
    62221 gcgcattgcc gctgggctat ccccgtcaat agacccgaaa ttaccctgcc catcaactag
    62281 cgggtaccgc atggaccaat cctgggccat cctcaccata gcatcgtaaa ccgaagaatc
    62341 gccatgggga tgatactttc ctaaaacctc ccctactata cgtgctgatt tcttgtaggg
    62401 atttcctgca ttcaaaccta aatcggacat tccgaacaat acccttcgat gaaccggctt
    62461 aagtccatcc cgtacgtcgg gcagtgctct tgatacgatc accgacattg agtagtcaat
    62521 gtacgatgac ttcatctcct cctcaatgtt aattctaata atcctctctg tttctgacat
    62581 atatactttt tataaaaagt aaattctgaa tatcgaacca aaaaaatttg aaagtgctaa
    62641 ggtagtaatt taatccattg tatagccctg aaaccctttt tcgtttttga gaaaactcac
    62701 gataatttaa taatttttat caacaaatcg cttgcttttc cgtttatcac tattatttta
    62761 gtaccaaatt taacttaagc aagtttttat gaaccaaaag ttttcacaaa aaatcagaga
    62821 tgtactttca tatagccgag aagaggctat aagacttggc agttccgcaa ttagctcaga
    62881 tcaccttttc ttgggtattc tgcgcgatgg agaaggcgaa gcaataaata ttttgctggc
    62941 cgaaggtgtt aatttaagcg agctgaaaaa ggaactggat gtccatttgc tgcaaatccc
    63001 aagtaaggca gaggaaataa acgactcatc ggaaattgca atttataagt cggccgaaag
    63061 ggcgttgaaa atagttattc ttgaggccag ggcattaaaa agtgagtcaa tcaatacagc
    63121 ccatctcctg cttgccatac ttaaggatga taccaactat gtaacccagc ttttaaagga
    63181 aaaggatatt gattacgaaa gggttaggaa aaatctgaca atattgaact cggacgataa
    63241 aggtgatttt ggttctggag atgaagatga gggggatagt ggttttttcg gtaacatggg
    63301 caaatccaac cagccctcaa caggaaaatc agcctccgac acccccgttc ttgacaattt
    63361 tggaattgac ttaactaaag ctgccgagga tggacgtctt gatcctatag ttggccgtga
    63421 aagggaaatt gaaagaattg cccaaatcct aagccgtcga aaaaagaata acccaattct
    63481 tattggagag cccggagttg gcaaatcagc tatagccgaa gggctggctc ttcgaattgt
    63541 aaagaaaaaa gtatccaggg ttcttttcaa caaacgggta atcacacttg atctggcttc
    63601 tattgttgcc gggaccaagt accgtggcca gttcgaagaa cggatgaagg cgatactcaa
    63661 cgagctatcg aggactccca atatcatcct gtttattgat gaaattcata ccattgttgg
    63721 tgcaggtgga gctacgggct ctctcgatgc tgccaatatg cttaaaccgg cacttgcaag
    63781 gggtgagctt cagtgcatag gtgcaactac acttgacgag tatagggagc atattgaaaa
    63841 agacggggct ttagagcgtc ggttccagaa agtaatgatt gaacctacct ctcctgagga
    63901 aacaatcgaa attcttaaca acatcaaaga tcggtacgag gagcaccata atgttaccta
    63961 cacacctcag gcaattgagg cttgtgtaaa gcttaccatg cgctacattt ccgacaggaa
    64021 tctaccggat aaggctatag atgccatgga cgaagctggc tcacgggttc atatctcgaa
    64081 tattcgtgta cccgatacta ttgttaacct agagaagctg attgaggaaa ccaaaaagga
    64141 gaaaataaaa gccgttaaga atcaacaatt tgagttggct gctaatctta gggataagga
    64201 gaaaaagctg attgagcagc ttgaggagga gcagtcgaga tgggaaaagg agctggctaa
    64261 gcatcgtgaa attgttgacg aggaaaaggt agccgaagtg gttgccatga tgacaggagt
    64321 ccctgtgcaa cgaatagcac aagctgaggg agccagactg ctcaaaatgg cagatgagct
    64381 aaggaattcg gtaattgggc aggatgaagc cattcagaag gtagttaagg ctatccaacg
    64441 aaaccgtgct ggacttaaag atcccaacaa acccatcggg acatttatct ttttggggcc
    64501 aacaggcgtt ggcaaaaccc agctagctaa agtgctcgcc aagtatcttt ttgatactac
    64561 cgataacctt attaggattg acatgagcga gtacatggaa aaattttccg tgtcacgttt
    64621 ggttggtgca cctccaggtt acataggata tgaggagggt gggcagctga ccgaaaaggt
    64681 tagaagaaag ccctactcgg ttgtgctgct agatgaggtt gaaaaagctc atcccgacgt
    64741 ttttcatatc ctgctccagg ttctggatga aggccagctt acagatagcc tgggcagacg
    64801 ggttgatttt aagaatacca tcatcatcat gacttctaac attggctccc gggagcttaa
    64861 ggattttggt caaggcgtgg gttttaccac caaagccaaa gaacaaagca gcagcagcga
    64921 ttattcaaga agtataattg aaaaagcgct taaaaaggcc tttgcaccgg agtttctgaa
    64981 tagaattgat gatattgtaa tgttcaactc gctaagtcgt gctgatattc ataaaattat
    65041 tgatattgag cttagcggcc tttatgaacg gattcgtaac ctgggattca caattagaat
    65101 ttctccggca gccaaggatt ttattgctga gaagggatat gatgttcagt atggtgctcg
    65161 ccctcttaaa cgagccatcc aaaaatacct tgaggatgcc ctggccgaag tgctcattaa
    65221 aagtgatacc aagcccggat gcacaatctc cgttggatat aacaaaaaga aggatgagat
    65281 ttccatcaaa gttcttaagg gggaagagga ggtaaccagc gctagtgaag aggagtagaa
    65341 cctgaaggct aatgaacaaa aaagtttcgg ctgtgttact accaaaatta catcactaaa
    65401 caccataaaa accatgaacg ttacttttgg aggaaaaaca gttacccttg aaggtgaaca
    65461 agttaaagtg ggctctactg ctcctgattt tacagcactc ggcaccgacc ttaaacctgt
    65521 caagctctct gattttggca aaaaagttaa gctaatttcc gtgttccctt ctatagatac
    65581 ggagatttgc tcaattcaaa atcatcgctt caataacgaa gcatcttcat ttggcgataa
    65641 ggttgctttt attgccattt cgaatgactt accctttgcc ttgaagcgat tctgcggagc
    65701 cgaaaaaatt accaacctta ccaccctatc cgatcacaag gatgttgatt ttggtaagaa
    65761 atttggtttt ttgataaagg agctacgctt gctgactaga ggggtgatta ttctggacaa
    65821 ggataacaaa gttcaatatg tggagatagt ccccgaaata gcctccgagc ccaactatac
    65881 tgaagcaata gctcgcctaa agcagctagt ttaaaaaaag ctcgacttgg aggtgctttt
    65941 ccaataaaag gaaattccca caagcaaaag aaaagcctta gaaaggtaat cattctaagg
    66001 cttatttttt atccacacca acctcacaag agctcagcac aactccccta tcaagtcata
    66061 atcgtctgca tcaacaattt taacattcac aaagtcgcct ggtttgagat tttgtgaata
    66121 gccatgaaga tggatattta catattggtc aacttcaggg gagtcaaact ccgatcggca
    66181 aataattatt tcatcttcta ccgaatcaac tataacacgt ataatgcttc caactctact
    66241 atgattgatc atgaacgaaa tgttgcgttg aagctccata agctcggagg cacgccgttc
    66301 cttttcgtcc gggggtatgg tatcggctaa atactgctgc gcataggtcc cctcctcctc
    66361 tgagtaggtg aataccccta ccctctctag cctaaactca tccacaaagc tcatcaactc
    66421 gtcaaaatcc tcttggtcct cacctgggtg gccaacaaga aacgttgttc taattgccac
    66481 acctggaata ttttgtcgca acttttcgac aagtgcaatg gtttccgatc gggagattcc
    66541 ccggtgcatg gctttcagca ccctgtcact aatatgttgt aggggtaaat caacatattt
    66601 gcaaaccttt ggattgtttg caatagtggc tataagatcg tctggaaaat gagcaggata
    66661 ggcatagtga agcctaatcc actcaattcc tttgatttgg ctcaaccgat cgattagctg
    66721 agcaattctc cgttccccat agatgtccat accatagtaa gttgaatcct gggcgataag
    66781 tatgagttct ttcacccctt tttctgatag gatggtagct tcttcaacaa gggattcgac
    66841 ggttcttgaa atatgcttac ctctaattaa cggaatggca cagtatgagc agccccagct
    66901 acatccctct gaaattttca ggtaagcata atggccgggt gttgtgagta acctggtggt
    66961 aagacacccg ctaaaaacgg gggtgttgag agcatggaga acggcgggga gctcggaaac
    67021 tccaaaaaat ccatccacct ccggaacttc atcctgcagc tcatttctgt atctttgagc
    67081 aagacaaccc ataacgaaaa ccttgtcaat gatgccctta tttttcgcat ctacaaagcc
    67141 catgatggta tcaacggact cttccttggc atcggcaata aacccgcaag tatttattat
    67201 caccacctta gcctcagtat cgttcgaatc gtgaacgacc tcccatcccc ccattttaag
    67261 ctgattcatg agtacttcgg aatcaaccaa atttttagag catcctaggg taaccacatt
    67321 aatttttccc tttcccggtt gttgcatact gctaattttt ctagctgaaa agggatgaaa
    67381 caaatgcgtt cttgtcaaaa acctgcaggt cagccagctt ttctccaacg ccaatgtact
    67441 taactggtat tttaaactgg tcggaaatgc ccagcacaac acctcccttg gctgttccat
    67501 cgagcttggt taggacgata ccggtaactt cagtagcagc aataaattcc ctggcttgag
    67561 cgaatgcgtt ttgtccggta gagccatcaa gaattaacag cacttcgtgt ggtgcatcgg
    67621 gaataacctt ctgcataact cgcctgattt tagtgagctc attcatcagg ttaaccttgt
    67681 tgtgcaacct acctgctgtg tcaataataa ctacgtcaga atcatttgcc tttgcagaat
    67741 tcaacgtgtc ataggctacc gccgatggat cggcgcccat ttgttgcctc acaatgggaa
    67801 catctaccct ctccgaccat actgtaagct ggtcaacggc tgcagcccta aacgtatctg
    67861 ctgcacctag taacaccttt ttgccagcta atttgtaaag atacgccagc ttgcctatgg
    67921 tggtagtttt acccactcca ttgacaccaa ccaccataat gacaaatggt tttgaaggca
    67981 gctccgtaat tgcacctgaa ttcaccggag tatcagctag caaagaaata atttcggttt
    68041 taagtattgt atttagctca ttggcattca ggtatttttc cttagccgct cgttgctgga
    68101 tctgctgaat aattttcaag gtggtctcaa ctccaacatc cgaggatatt agagcttcct
    68161 ccagcttatc gagcacttcg tcgtcaattc tagattttcc aacaacagcc cttgaaactt
    68221 tcttcaacaa gctttctcgg gttttttcca gccccttatc gagattttcc cggctgttgc
    68281 ccttacccgt tgaaaaaata tttggtattc ccatttcaaa ccttgttaag tgctatgaat
    68341 atggtattgg ttataaaaga taaaaaaagc tttccttttg caagaaaagc ctaaatacgg
    68401 tgatacattt ggagaagcac acaggcttat ttcttcaaat atgcatcgat ttcggtattg
    68461 gcaaccattt cttccttaaa ggtgtaagca cctgttttgg gtgattttac cattttaatg
    68521 caacgggtaa aatttttagc acccttttgc tgcatagaag caactacttt ttttgccatg
    68581 gctctactat tttatttcac gatgaacagt taccctctta agaataggat tgtacttgcg
    68641 acgttcaagc ctatcagggg tgtttttctt attctttgta gttatatacc tggaagttcc
    68701 cggcattccg ctttccttgt gctcagtaca ttcaagtatg acctgaaccc tgttaccctt
    68761 cttagccatt tgttaacaga tttagtaggt tttaagaatt cctttttcta cagctttatt
    68821 aagcgtagct ttcagtccat tcttattgat tgttcgtatt gcagcagcag aaaccttgag
    68881 ggtaacccaa cgattttcct cctcaaggaa aaaccttttt ttcttgagat tagggtaaaa
    68941 tctgcgctta gtcctccttt tagaatggga tacattgttt cccaccattt tcttctttcc
    69001 ggttatttga caaactctcg acatcgtaat attgttctaa gtaagttttc taaacaggtc
    69061 gcaaatttcg gcaaaattat gaatttatca aactttttca aaaatatttt tcaactccgg
    69121 gtgttctttt agcagtaact ttcgaagcat gttcagcgct gagtatgatg ccctttggat
    69181 atttatggat cggtcgttgc tgaaaatata tttttcaact tccagggagt ttaccgaggc
    69241 agctgcaatc catacaagcc cagctggttt atccggtgtt cctccatcag gacctgctat
    69301 tcccgaaacg gctatggagt aatctgtgga aagtagattt aaagagccaa ccgccatttc
    69361 ttctactgcc tcacgactaa cagcaccata cttaattagt gtttgatgtt tcactccaag
    69421 gagattttcc tttacctggt tggagtaggc gacaacaccg ccattaaaat agctagaagc
    69481 acccggaatt tctacgataa ggtgtgaaat ttttcctcct gtgcagctct ccgctacaga
    69541 aaccgttgca ttgcagctta atagcatttt cccaaccact tcaggaagtg aagtttcacc
    69601 atacccaaag atatactgag gaattgttgc ccggagtttt tctatagctt catcaacctg
    69661 ttgtgataag agcttcctat cgtttccata agccgaaagg cgaagtcgaa tgcccgaagg
    69721 gtcaggcaga taggcaagct taatggaagg tgaaatgctc ttttcgaact cattaagcac
    69781 atccgaaagg tttgactcgg caatgccaaa ggtatgtata accttatgaa taataacacc
    69841 ttcaactggg agttgcttta gcaagggagt aacctcattt tcaaaaagtg ccttcatttc
    69901 gaagggtaca cctggaaggg aaaccagcat atggattccc tttttaaaca acatgccagg
    69961 agctgttcca aaatagttag gtagtacagt gcaggaagat ggaacatccg cctgcttacg
    70021 gtttgtttcg gttaccgcta taccacgttt agagcagaaa atttcaataa gttctaacga
    70081 ttgggcgtcg gttatcatct cgccaccaaa tattcttctc aaactttcct tggttacatc
    70141 atcgctggtt ggccctagcc ctccggtaac tatggttaaa tcgtaccggt caatagcttc
    70201 atttaccgat tcgcatatag cctgctcggt gtccgacacg gatagtatcc tttcaacagc
    70261 aaatcccagg ctattgagcc ttgtggcaat ccacgcagag tttgtatcaa caacctgccc
    70321 tattagtagc tcgtcgccta tggtaattat cccggcattc ataaggccaa tgaaatttat
    70381 tcaggtagaa ataaaaaccg gacaacggtt tgctgtccga ctggagcctc tcacgggact
    70441 tgaacccgcg acctgcggtt tacgaaaccg ctgctctacc agctgagcta aagaggcatt
    70501 tgctgcaaaa atacagcttt tttagagact tccaacttga agcgaccttc catcttttaa
    70561 aaatgaaacc ctgccaaaac agctaaaatg gagaaagaag taggggttag tttttcaatt
    70621 tcgaaaacac tattaatcat ataacactta ctatcaactg aatataccga tttatacaaa
    70681 ctaatagtta aataatttaa tgggaagtgc agaacctgtg gtagcttacc aaagctaaaa
    70741 tcaaggtttt gtcgaattgg ctttaattct atagttaatt atttccatgt aatcatttaa
    70801 gccttcgaga ttgggattta gacgcttagc ctgcatcagc cattctaagg aaacgttaaa
    70861 atttccgagc atttcatttg caagagccat gttgaatgca gccctggatg gaagtgcttt
    70921 gcccccattt ttaattactt gctgccatat ttcagctgct ccacgccaat cacccttaag
    70981 ggcaagtttt tctgcctcca ccatttgctt tgaaccgctg ttgtaaagga ttcgggtact
    71041 attttcccag aaaggggcga tttgggcagc aaacttttct gcagcatcag cgccactata
    71101 ggccacggcg gcaaagtatc ccggcagctg atctccgggt ttaacctcac gccaatcgtc
    71161 agcttcccat accagcgtgt cccgatgcag gaaggaatta gtaatttttt ggtttgtaat
    71221 atcgtaaacc ctccagtagc agtaaacggg aacatagata taaccatgat aggcattcac
    71281 gctaccctgc cataacgtat agctgcctgc tgtaggctta acattcatgt aatcaagtga
    71341 aaggataagc cctgctgaat cgtttatttc agataagttt ttcagctgtg tccaaggcag
    71401 gggtaaaatc atattggaag tatccttacg gtaaaccgca tagtggctga agcttgtctg
    71461 gctgaatgct ggcgaagagt tcaatacctt aaccagcgat aaggttgcct cagtagcggc
    71521 tacactatct aatgcccatg caagtgtttc tcccccacta ctatggcttt taccttgata
    71581 gtttgtaagg ataaggactt tgccattacc ggttggtatc gatattttgg tagacgagtc
    71641 gtagtactcg atatcaatct gcctgtaggg aatacatgcc gaaacgaaaa gtagtagaaa
    71701 aagtccgcaa aacctaattc ttcccattgg taagaatatt actaaaaata gctgaattga
    71761 tgctatttag cggagatgtg acttccaaac aaaaaataaa cagcatcaat tcaagcagaa
    71821 ttcgatatta acaagtggta ctatgtattc attccaccac agacatgaat aacctgtccg
    71881 ctaacatagg acgaaagatc tgaaccaagg aaaatgcaaa cattggccac atcctcagga
    71941 gttccacccc gctgaagtgg aattttcttg atccaatcct gacgaacctc ctcggaaagc
    72001 tggtgggtca tatcggtgat gatgaacccg ggagcaacgg catttgagcg gatatttctt
    72061 gagccaagct cctttgcaac cgacttggta aatccgatta taccagcttt tgaagcggca
    72121 tagtttgcct gacccgcgtt acccgaaacg cccactaccg agctcatatt aataattgaa
    72181 ccgctttttt gcttaatcat gtacttctga acagccttgg tcaggttaaa tactgacttc
    72241 aggttgaccc taatcactaa atcccactgt tcttcagaca ttctcataag tagggtatca
    72301 cgggtaatac cagcattgtt aaccagaatg tcaattctgc caaactcggt tgcaatctga
    72361 tcaacaacca tttgggaatt ttcatggtta cttgcatctg aaacgtaacc cttagcccta
    72421 acaccaagag catttagttc gcgttcaagg ctcttcatgt tgtcatcttc caccatatcg
    72481 gtaaaggcta catttgaccc ctcacgtgca taagcaagtg caattgccct gccaattccc
    72541 ctggcagcac ctgtaattag gctggttttt ccttcaagta atttcatggt taaatgaatt
    72601 tttgttgtcc cgacaaagtt aaaaaaatcc cctattatgg aagagctgta gcaagcaaca
    72661 tgccataaca gtatttgaaa attttgttac agcaatgacg gaatttacaa tatttatgag
    72721 tatgtgcttt agtttcaggc ttcaactatt gagttataat tattttacca catgagatga
    72781 atgggaaact gcatatggat aggttcgaaa aataagcaca tagcaaataa gcttatttta
    72841 cccagcttgc gagttcaata tttttttttg aaatgtaaat atcctatatt cacgctttaa
    72901 acgttttgaa aaacattggt tatagactat tgataatggc atacatacct gccattttta
    72961 aggtaaccct tcaatggatc tgtagcggta ttttgatacg aacaagtagt aatgcataaa
    73021 gagtataaaa aatgaaagtt aggttgaagc taaggagcaa actgcttctt tcagtgttat
    73081 cgactgccat agtggtattt ctagtttcat ttggatacct gactaataaa cttgaagaca
    73141 tcacccttcg tagtgctttc cgctatgtgg atgcaacaac aagagagcac tccaatgccg
    73201 ttaaggcgaa ctttgatgag gagatgggca tggcaagagc aatggctcaa gggtttcaga
    73261 attacaagga tttaaactat gaggagataa ataaaaattc gctaagagta ctacagggga
    73321 tagcagttaa taatccccaa tttttatcgg tttgggccag ctgggagtta aactctatag
    73381 ataggacatg ggataagcca tacggaagag tacgatacac ctatttctgg gaaaatgggg
    73441 cgcttaagta tgtagaagat agggtaaatt tgacagggga cgatcctaac agctcatact
    73501 accacattaa gtcaaccaaa agagaattta taattgaacc gtactggttt acctatactg
    73561 ggactaatat gaaagtgctc gaaaccagca tttgtgtacc gctggttgac aatgaccggt
    73621 tcgttgctct atttggattt gatattgagc ttgaacgtta ccatgaattt attgacagcg
    73681 ttgaaatatt ccctggtagc aattcgatgt tgatttccca agatggtttg atagttgcgc
    73741 atagcgattc aagtctgacc gggattcgtg tagatagcat ttttaaccaa caaaaaattg
    73801 gctttaacat catagataag cttaaaaagg aatctgattt ctcctttaac gttaacatga
    73861 atgggagtga atactatgca tcttttcaat catttgcaat tagtggaaca acaaaccggt
    73921 ggaccttcgc ctcactttca cctctatcca ttattgttga agagtcgaag gatgtagcaa
    73981 cacgctccag aatagtcata ttagccgggt tgctgattct tgcactggtt gtatggatta
    74041 tttcctacag cattacccac ccattggtaa aagccaccag aaccctcaga gaacttgctg
    74101 gaggtgaaat agacacgagt aaaaagctag ctatcaactc tggagatgag gttgaggata
    74161 taggaagctc aattaatatt ctaattgata gcctatataa aacaaccaac tttgcaaggg
    74221 aaatagggaa aggcaatttt aatgttgact acagcaagtt aagcgaaaac gataagcttg
    74281 gtgaagccct actagatatg aggaaaagtc tggagcatgc cagactacag gaagttgagc
    74341 gtaagctagg tgaagataag aacagatggg caaatgaagg cgtggctaaa tttgccgaga
    74401 tacttcgtca aaatagcgat gatctgaacg aattttgcta ctcgtttatc tacaaccttg
    74461 ttaagtatgt tgatgctaac ataggtgcaa tatacctggt aaataatgac aaccctaatg
    74521 aggtttactt cgagctaatg gctacctatg catacgagcg taggaagtac gaggaaaaaa
    74581 gaatagattt cggtgaaggg ttagttggaa gatgcgctaa ggaagccgaa accatttaca
    74641 tgaccgacct gccaaaagat tacataaaaa tttcctccgg acttggggag gaatccccta
    74701 catgtctcct tctagtgcca ataaagctaa atgaaaatgt ccatggggtt attgaaatgg
    74761 cttcatttga gcctatagag gattataaaa tcaggtttat agagaagatt ggagaaagct
    74821 tggctgcaac aatatcaaat gtaaaaatca acatccgtac aggaaagttg ctggatgagt
    74881 cccgggttaa atcagaagag ctggcttctc aggaggagga aatgaggcaa aacatggagg
    74941 aacttcaagc cacccaggag gaagcagcta gaaaaacagc cgaaatggaa agcctcatcg
    75001 gagcattaca tgcctcaagc tttgtaattg agtatgattt gaacggcaat attacttctg
    75061 taaaccaggc ttaccttaca ctaactggac agtcagagaa tgaagttatt ggcacccacc
    75121 attctgataa tctggaaatg actgatgagc aaaagaaaaa ctatcagaag ttctggaacg
    75181 atttgcgtaa cgggatggtt aaaaaggaaa ccagccgtat taagcttgga aagaaaacgc
    75241 ttacctttat tgaaacctac tcgccaatct tcaaccaaaa ccggcaggtt gttaaaatcc
    75301 tgaagattgc acacaacata accgacttta tagaggacga gaaagttaaa ggtcgaaaaa
    75361 cgaaataaaa aaaagagagt cgcttatcag cgactctctt ttttagtact atccattagc
    75421 aag
//
LOCUS       DAOSHM010000004        70536 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_303_length_70536_cov_20.559413, whole genome shotgun sequence.
ACCESSION   DAOSHM010000004 DAOSHM010000000
VERSION     DAOSHM010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 70536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 70536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70536
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_303_length_70536_cov_20.559413"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            367..1242
                     /locus_tag="PK839_01195"
     CDS             367..1242
                     /locus_tag="PK839_01195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG71378.1"
                     /translation="MIHVATVHWNTDRWVDLQLNQLNRYIHQPFRVYAFLSGEAAKQR
                     SKYFYSSCQPITEHAVKLNLLAETIMLLAQSDEDPLIFIDGDAFPVTPLDDFIGMQLE
                     RYPLAAVQRLENVGDIQPHPSFCLTTVGFWKSISGDWLKGYEWTNSRGVKRTDVGGNL
                     LKILNDLGVEWKPLLRTGQLSEHPLMFGIYENAIYHHGAGFRQPLSMYDRLKPTSHVV
                     KNKAVAGGIDLVLDKLPLKNRFFLQRHLGLKKNILRQNVELSEAIYHDFASIISKGEA
                     YDFISKSRRFPVTGS"
     gene            complement(1267..1788)
                     /locus_tag="PK839_01200"
     CDS             complement(1267..1788)
                     /locus_tag="PK839_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018335856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HOG71379.1"
                     /translation="MDTLQDFVPVSPYELKDNFFDAVDNGWMLITAGDEQKFNTMTAS
                     WGSFGILWNMPIAQVFIRPQRYTFQFVEQHSYYTLSFFDEKWRKALNFCGAKSGRKFD
                     KPKETGLVPATTGHGIAFQQARMVLECRKLYADNLKPEQFIDRDIIRKNYPKSDFHRF
                     FIGEIVNVYLARG"
     gene            complement(1799..2680)
                     /locus_tag="PK839_01205"
     CDS             complement(1799..2680)
                     /locus_tag="PK839_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - cellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HOG71380.1"
                     /translation="MNETLSKEEFSNLILKVQRDEINGFKIYSKLAKIEKDASNREIL
                     SRIAAEEYSHYNFFKSISGREVGHNRLTTWFYFTLTHLFGLTFGIKLMERQEDKAQRV
                     YPQLLPYIPEIDGILRAEEEHESELIEMIKEEKLDYAGSIVLGLNDALVELTGALAGF
                     SFAMQNTRLIAIAGLITGIAASLSMAASQYISVSTENSGQNALKSSIYTGIAYVITVI
                     ILIAPYLILQSYFAALALTLTFAILIILVFNYYISVAKDLNFKRRFSEMAIISLGVAA
                     LTFGIGFLIRKYLGVDI"
     gene            complement(2829..3587)
                     /locus_tag="PK839_01210"
     CDS             complement(2829..3587)
                     /locus_tag="PK839_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOG71381.1"
                     /translation="MKIALITGATSGIGRATALRLAQDNFNLIVTGRRLTQLEQLKKE
                     VEVKFKKEVHILNFDIRFREQVDSAIDSLPEHWRKIDVLVNNAGLALGLNHLQDGMVE
                     DWEQMIDTNIKGVLYITRKVAPLMIERGGGHIVNIGSTAGTQVYENGNVYCATKHAMH
                     ALSQSMRQDMLKHNIRVTEVRPGLLETEFSLVRFKGDKKKAKDTYAGLTPLSAEDIAE
                     VIYFALTRPPHVCINDIEVTPTAQANSYYTNRKV"
     gene            4547..5902
                     /locus_tag="PK839_01215"
     CDS             4547..5902
                     /locus_tag="PK839_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit A"
                     /protein_id="HOG71382.1"
                     /translation="MSTIVKVRRGLNIPMEGRAENVLVRKNMSETYGVKPLDFPGLLP
                     KLEVQEGDVVRAGTPLFYDKYRPEVKFSSPVSGEVVEIRRGERRQILEVVVKANGSVE
                     YQVFGAAQPAALTRNQVVEKLLVSGVWPYIRQRPFGIVANPNDTPKAIFISCFDTAPL
                     APDMDFSIQGEDENFQAGIDALSKLTPGKIHLGLNADYPAASAFAKANGVEHHYFRGP
                     HPAGNVGVHIHHIDPIGKGDVVWVVNPLDVVIIGRLFLKGIYDASRVIALAGSEVTSP
                     RYYRVISGAQIDSITSGNLNSNIELRYISGNVLTGTKVEPFGYLGFYDNMVTVIPEGN
                     HYEFFGWMSPGFNKFSASRTFLSRLTPWRKFKFDTNLHGGKRAYVITGQYEKVMPMDI
                     LPVHLIKAILANDIERMEQLGIYEVVEEDMALCEYVCTSKTDVQQILRKGIESLIKEL
                     S"
     gene            5925..7088
                     /locus_tag="PK839_01220"
     CDS             5925..7088
                     /locus_tag="PK839_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HOG71383.1"
                     /translation="MGLRSYIDKIKPNFQKGAKYGMFHSTFDAFETFLFVPNRVTTKG
                     SHIRDSIDMKRTMTVVIVALLPALFFGMWNVGYQHALSIGESWPFWQTFFFGFLRVLP
                     LLVVSYGVGLSVEFAFAQARGHEVNEGFLVSGLLIPLILPVDVPLWMVAIATAFAVIV
                     GKEVFGGTGMNIFNPALLARAFLFFAYPSHMSGDKVWIDGLAKGQQVVDGFSGATPLA
                     NAAAYQLDKIPSAYDMFMGFIPGSIGETSKLAILIGAILLIITGIGSWKIIISVFGGG
                     LVAGLLFNTLAINPYMSIPPWQHFLMGGFAFGAVFMATDPVSASQTERGKWIYGFLVG
                     FMAILVRVLNPAYPEGMMLSILLMNAFAPLIDYYVIQGNIRRRLKRTNRVAKS"
     gene            7127..7864
                     /gene="nqrC"
                     /locus_tag="PK839_01225"
     CDS             7127..7864
                     /gene="nqrC"
                     /locus_tag="PK839_01225"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit C"
                     /protein_id="HOG71384.1"
                     /translation="MDKNSNLYTILYASGLVVLVAVLLALAATLLKPAQQRNVETEKK
                     IDILKSIGKADGVEKAANKHKFVASNFERYIVEQLVVNSLGERIQGVDAFNVDLKVEL
                     SKPLENRYLPVFVAKLDSGSTKFIFPLRGKGLWGPIWGYISLNDDFNTIYGATFSHQG
                     ETPGLGAEIATPQFQKQFDGKSIFDNQNQLVSIKVVKGGADKSDPHGVDAVSGGTITS
                     KGLEAMLYDCLSIYMPFINNQKQQSHE"
     gene            7857..8516
                     /locus_tag="PK839_01230"
     CDS             7857..8516
                     /locus_tag="PK839_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HOG71385.1"
                     /translation="MSDVKEKEPWFSAKNIELLTGPLNSKNPITVLVLGICSALAVTV
                     KLEPAIVMAISVTVVTAFSNLIISLIRNTIPSRIRIIVQLVVIAALVILVDEVLKAYV
                     YDVSKQLSVFVGLIITNCIVMGRLEAFALGNKPWPAFLDGIGNGLGYGLILVVLAFFR
                     ELLGSGTIFGYRVIPESFYYSNGGMYENNGLMILPPMALILVGLIIWIQRGRNRKLIE
                     E"
     gene            8536..9153
                     /gene="nqrE"
                     /locus_tag="PK839_01235"
     CDS             8536..9153
                     /gene="nqrE"
                     /locus_tag="PK839_01235"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HOG71386.1"
                     /translation="MENLINIFVRSIFIDNMIFAYFLGMCSYLAVSKTVKTSTGLGIA
                     VIFVMLITVPVNYLLNRFVLLPGALQWLDESFASLDLSFLSFIMFIAVIASIVQLVEM
                     VVEKYSPALYNSLGIFLPLIAVNCAIMGGSLFMQQREYETLGEATVFGLGSGVGWFLA
                     IISIAAIRERLKYSDIPKPLRGLGISFILTGLMGIAFMTFMGIKL"
     gene            9173..10432
                     /gene="nqrF"
                     /locus_tag="PK839_01240"
     CDS             9173..10432
                     /gene="nqrF"
                     /locus_tag="PK839_01240"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HOG71387.1"
                     /translation="MILLAGNSSIILGAVIVFLIVTLLLVLLLLYAKAKLTPKGKVKL
                     EINNEKVLEVEPGSSILSTLSNNKIFLPSACGGGGTCGLCRCRVVEGGGSMLPTEANF
                     FNRRQQREHWRLGCQVKVREDLSIIVPEEVLGIKKWECEVISNRNVATYIKEFVVKLP
                     DGEKLNFRSGGYIQIDVPKIEVDFSKDIFVEPEFHDDWDKTNMWSLKMKNTEETFRAY
                     SMANHPAENNIIMLNVRIATPPWDRERNTFLKVNPGVCSSYIFSRKPGDKVTISGPYG
                     DFFIKDTQNEIMFIGGGAGMAPMRSHIFHLFNTLKTNRKVTFWYGARSRREIFYEEHF
                     RKIAQEFPNFNFTIALSEPKPEDSWDGPVGFIHQVIFEQYLSRHNAPEDIEYYLCGPP
                     AMIAAVQKMLYNLGVPDEMIAFDDFGG"
     gene            10692..10928
                     /locus_tag="PK839_01245"
     CDS             10692..10928
                     /locus_tag="PK839_01245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG71388.1"
                     /translation="MAIVKLIGITVGFLMLGMLGLGIRVIFHRSHKFPETSAGHNPEL
                     RKRGISCPKSDEQTCWGGENSYTGCSDCLERSKH"
     gene            11021..11206
                     /locus_tag="PK839_01250"
     CDS             11021..11206
                     /locus_tag="PK839_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG71389.1"
                     /translation="MGTLKLILISAAMLAVVIVGLSLKLIFGKTANKSCKSNDVGYSC
                     GCGGSCGINSPNIGEQL"
     gene            11740..12033
                     /locus_tag="PK839_01255"
     CDS             11740..12033
                     /locus_tag="PK839_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG71390.1"
                     /translation="MNVDSSEIYDRIKQKVQVLVVAYERAKQENEQLKAQKAELEKQL
                     KAKEQAYVELEERYSRLKLAKALKVSEADAHEAKIKVNRIVREIDKCIAFLNK"
     gene            12049..12363
                     /locus_tag="PK839_01260"
     CDS             12049..12363
                     /locus_tag="PK839_01260"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HOG71391.1"
                     /translation="MEDSENKFSIRVNIADRYYPLKIDWDDEEKIRRAAKLINDKVLQ
                     YKQRYTDKDIQDFLAMASLQFVIRLIECEEKFSGAAFDEELSKLDDWLAAYIEKQNIR
                     SF"
     gene            12600..14135
                     /gene="rny"
                     /locus_tag="PK839_01265"
     CDS             12600..14135
                     /gene="rny"
                     /locus_tag="PK839_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005790424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HOG71392.1"
                     /translation="MELTIIVAVIAFILGGAISYLLWNKLLASKRSKIIQDAETEAEV
                     IKKEKILQAKEKFLQLKAEHEKIVSERNSRIAQAENKIKQRETMVAQKYEELQRKQKE
                     VDAIRDTLTTQMELVDKRNEELERMHRESVEKLEALSGLSAEEAKTQLIDSLKAEAKT
                     QAMSYINEIVDEAKMTAAKESKRIVVQTIQRVATEAAVENSVTVFNIDSDEIKGRIIG
                     REGRNIRALEAATGVEIIVDDTPEAIILSAFDPVRREIARLALHQLVTDGRIHPARIE
                     EVVQKVQKQIEDEIVEIGKRTTIDLGIHGLHPELIRLVGKMKYRSSYGQNLLQHSREV
                     ANLCAIMASELGLNAKAAKRAGLLHDIGKVPDDEPELPHAILGMKLAEKYKEKPEICN
                     AIGSHHDEVEMTTLIAPIVQVCDAISGARPGARREVVESYIKRLKEMEDLALSYPGVL
                     KTYAIQAGRELRVIVGSEKVSDEEANKLSFEIARKIQDEMTYPGQVKITVIRETRAIS
                     YAK"
     gene            14336..15475
                     /locus_tag="PK839_01270"
     CDS             14336..15475
                     /locus_tag="PK839_01270"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOG71393.1"
                     /translation="MNNSSIQMCDLRGQYLKIKPEIDAAIQQVIDSTAFIKGKEVGDF
                     QDELARYLGVKHVIGCANGTDALQVALMALGLKPGDEVITPDFTFIATVEVVALLGLT
                     PILVDVDPFTFTIDTKKLEEAITPKTKAIIPVHLFGQCANMGEIMRIAQIHNLYVVED
                     AAQALGASYYHSDGHVSKAGTIGHIGATSFFPSKNLGCYGDGGALFTNDDSLAAEIRS
                     ITNHGMRVRYHHDRIGVNSRLDTVQAAILRVKLKYLDSYNEARMAAAQLYDTRLKVCA
                     NLVIPSRATWSNHIFHQYTLRVNEANREELSKFLAGKGIPSMVYYPIPLHSQKAFEAY
                     RLQNSNSTFPVSNSLSTSVLSIPMHTELDVDTINFITESILSFFK"
     gene            15505..16083
                     /locus_tag="PK839_01275"
     CDS             15505..16083
                     /locus_tag="PK839_01275"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008149867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOG71394.1"
                     /translation="MEVHYFAHETAVIDSDAKIGQGTKIWHFSHIMSGSVIGENCNIG
                     QNVVVSPGVTLGRNVKVQNNVSIYSGVTCEDDVFLGPSMVFTNVINPRSAINRKNEYM
                     PTIVKRAATIGANATIVCGVTLGEFCFVGAGAVVTKDVKPYALVVGNPARQIGWMSEF
                     GHRLEFNSDNLATCPESGQVYRLDNNQVNKIS"
     gene            16087..17025
                     /locus_tag="PK839_01280"
     CDS             16087..17025
                     /locus_tag="PK839_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018965270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOG71395.1"
                     /translation="MKNFALIGAAGFVAPRHLKAIKETGNTLLAALDKHDCVGIMDSY
                     FPQADFFVEYERFDRHIDKLKRQGIKVDYVSICTPNYLHDSHIRFALRQGADAICEKP
                     VVLNPWNIDALSEIEHETGRHIYTILQLRLHPAIIEFKKKIDNGPKDKIYDVDLTYIT
                     SRGRWYFISWKGDVQKSGGVATNIGVHFFDMLTWIFGDVKSGVVHMSMPDKAAGYLEL
                     QKARVRWFLSIDYNDIPDTVKQQGKRTYRSITVGNDELEFSEGFTDLHTTSYRDILSG
                     NGFGLNDARSSIETVYAIRNAVPVGLKGDYHPFCKK"
     gene            17049..18341
                     /locus_tag="PK839_01285"
     CDS             17049..18341
                     /locus_tag="PK839_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008771971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HOG71396.1"
                     /translation="MFKELVSKNEKMAVIGLGYVGLPIALEFAKKISVVGFDIKPDRV
                     ELMKRGVDPSHELESTSFQNCDITFTSDINDLRKASFYVVAVPTPIDEHNLPDLKPLL
                     AATRTVGQVLKKGDYVVYESTVYPGCTEEDCVPILEELSGLKFIQDFKVGFSPERINP
                     GDKNHTLTTIKKITSGCDPESAEEIAKTYELIIKAGIHRASSIKVAEAAKIIENTQRD
                     INIAFMNELSIIFSRMGINTYEVLEAAGTKWNFLNFSPGLVGGHCIGVDPYYLVYKAK
                     ELGYHPQIISAGRYINDTMGGYVAKQTVKKIIAAGTNPMDARVLVMGVTFKENVSDIR
                     NSKVVDIYRELRSFGVNYIDIIDPYADAHDVKHEYGFDMATAPSGRYDAIILAVGHSQ
                     YAGLKEEWFKRLAAEKCVFVDVKGIFRSKINNLTYWSL"
     gene            18533..20473
                     /gene="thrS"
                     /locus_tag="PK839_01290"
     CDS             18533..20473
                     /gene="thrS"
                     /locus_tag="PK839_01290"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HOG71397.1"
                     /translation="MVKITFPDGSIREYPLGVTGIEIARSISERLAAEVLSITVNGEV
                     WDLTRGITTDATVKLHKWDDEEGKHTFWHTSAHLMAQALEALYPGIKLGIGPSIDNGF
                     YYDIDFDSYTISDDDFPKIEQKMAELAHQKLDVVRKDVSKDEALATYKAKGDPYKVEL
                     ISDLQDGTITFYTQGDFTDLCRGPHLPNTGYIKAIKILSIAGAYWRGNEKNKMLTRIY
                     GITFPQKKLLDEYLVMLEEAKKRDHRKLGKEMELFTFSQHVGQGLPLWLPRGAQLRFQ
                     LEMFLRKVQQKYGYEQVITPHIGQKELYEISGHWAKYGKDSFRPITTPQEGEEFLLKP
                     MNCPHHCEIYKSKPRSYKDLPVRLAEFGTVYRYEQSGELHGLTRVRGFTQDDAHIFCR
                     PDQLKQEFVKVIDIVLYIFKTLNFKEYTAQVSLRDPKDREKYIGTDENWEKAESAIIE
                     AAAERGLKTTTVVGEAAFYGPKLDFMVNDAIGRKWQLGTIQVDYNLPERFQLEYIGSD
                     DKRYRPVMIHRAPFGSMERFVAVLLEHTGGRLPIWLAPDQVVVLPISEKYSDYAKKVF
                     NYLNNSDIRTLIDDRNEKIGKKIRDNELRHIPYLLIVGEKEEENGTVAVRVQGEGDKG
                     QMSLDDFIMLVRNNIESQLTEI"
     gene            20503..21057
                     /gene="infC"
                     /locus_tag="PK839_01295"
     CDS             20503..21057
                     /gene="infC"
                     /locus_tag="PK839_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008860711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HOG71398.1"
                     /translation="MATPLHSQVKKDVDNKFLVNNEIRNKVVRLVGENIENPGIFSIE
                     EARRLANELELDLVMISDKADPPVCRIIDYQKFLYQQKKKQKEIKANAQKIVVKEIRF
                     GPNTDEHDYNFKLKHAQNFLKEGAKVKAYVFFKGRSILFKEQGEILLLRFAQDLEDLG
                     KVEQLPRLEGKRMIMLISPKGAKK"
     gene            21084..21281
                     /gene="rpmI"
                     /locus_tag="PK839_01300"
     CDS             21084..21281
                     /gene="rpmI"
                     /locus_tag="PK839_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004584226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HOG71399.1"
                     /translation="MPKIKTHSGAKKRFSLTGTGKIKRKHAYKSHILTKKETKQKRNL
                     THFTLVDKADEKRVKKMLGLR"
     gene            21390..21734
                     /gene="rplT"
                     /locus_tag="PK839_01305"
     CDS             21390..21734
                     /gene="rplT"
                     /locus_tag="PK839_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004333375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HOG71400.1"
                     /translation="MPRSVNAVASRKRRKKILKQTKGNFLARRNVYTVAKNTLEKGLT
                     YAYRDRKKKKIEFRALWIQRINAAVRPYGLTYSEFMGKINKKGIAINRKVLADLAMNN
                     PEAFKSIVDSVK"
     gene            21870..22589
                     /gene="dapB"
                     /locus_tag="PK839_01310"
     CDS             21870..22589
                     /gene="dapB"
                     /locus_tag="PK839_01310"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HOG71401.1"
                     /translation="MRLAIIGYGKMGHEVESMAMERGHEVTLRIDRDNTHELTPENLK
                     LVDVAVEFTSPKSAKANVEVCLNAGVPVVCGTTGWGNELEAVIERVKVGGGTLFYASN
                     YSVGVNVMFKISALLARYLDKIGGYSVGIKEIHHLQKLDAPSGTAITIAGKITEEIPS
                     ITGWTLLPKREDGKIPIEAIREGTVPGTHTISFSSDQDSILLTHEAKSRRGFALGAVM
                     AAEFLHNKVGFFTMDDLLAFD"
     gene            22769..24094
                     /gene="lepB"
                     /locus_tag="PK839_01315"
     CDS             22769..24094
                     /gene="lepB"
                     /locus_tag="PK839_01315"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOG71402.1"
                     /translation="MRTLFKNRYFNLGLSSLIYILMVIWIGNYWLLLGLAVIFDFYIT
                     RKVNWTFWKKRGQKRTVLIEWVDAIVFAVIAALLIRMFLIEAYTIPTSSMEKTLMVGD
                     YLFVSKVSYGPKMPNTPISFPFVHNTLPFSKKAKSYVEWPRWPYKRLAGLGSIKRNDV
                     VVFNFPEGDTVVLQDPNSSYYTLARFYGRDYLWQNFDIVYRPVDKRDNYIKRCVAIPG
                     DTVRVVHGQLYVNGKEQVKIGERQYNYRIITNGGTINPKRFEELGIAKADQNYNPAES
                     EYIIPLTEGMLDRIKDYGNIVKIERMENTDSTRMWQMIFPHNPSYPWTEDNFGPLWIP
                     SKGATVGLNLDNLPLYERIIDVYENNDLAVRNGAIYINGKPSNSYTFKMDYYFMMGDN
                     RHNSADSRFWGFVPEDHVVGKGSFIWLSLDKDRHFPFNIRWKRIFSGIH"
     gene            24209..24844
                     /locus_tag="PK839_01320"
     CDS             24209..24844
                     /locus_tag="PK839_01320"
                     /inference="COORDINATES: protein motif:HMM:NF020463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HOG71403.1"
                     /translation="MDRKVVLSTAYLPPIQYFAKLLAYDVVYIESAENYAKQSYRNRC
                     TILSANGPLNLSVPAVKSLGNHTPITKVGIDNSSEWQKNHWRAVESAYRSSPFFDFGA
                     DLLLPFYTQRFENLFELNMELLKVLLGFIGVVVDVRFTTTYLTSYPEEYHDCRYFIHP
                     KRSFKADDHMVWVEYYQVFSDRFGFVPNLSVIDLIFNEGLGATSILKSMNK"
     gene            complement(24898..26433)
                     /locus_tag="PK839_01325"
     CDS             complement(24898..26433)
                     /locus_tag="PK839_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG71404.1"
                     /translation="MIRHISRFFLVLMASGTVTQLFSQTIEDGIRFSQTLNGGTARSI
                     SLGGAIGALGGDFTSLSINPAGLGVYKSGELTVTPSFKNQKVSSTYNGIKATDTRNRL
                     YFDNVGVVMSLKPNGSDDKGLVNLNMGVGYTRTNDFFSNSIATGDNPNNSIMDFFAVK
                     AKGYYYNDLLVDDNYNPFSESSAPWDVIMAWNTYLLYDTIAGTNGTEYDPALLIGDGV
                     VQKNAVSTKGGSGEFDISFAANISNKLYLGLTVGIADYSFTYNATYSEDAFQSNPARS
                     NGDRFYYSDYMQYFETSGTGYNIKLGAIYTPIPSIRIGAAIHTPTFFSFSDSYSYTMK
                     SNFDIAGVESNFTSNSPLGKYDYDFETPFKLVGSFAYIIKEYGLVSLDVEHLDYSAMR
                     FRDGGNGDNFTDLNQSIDGHFRNVTNIRVGGEARINDFSVRAGYAYYPSPYKSGFLND
                     NSNTNQLSFGLGYRIGNFTIDAAYLQTLQNSKYKFYDLTDLNVVSTKTRDGKFLITLG
                     FRF"
     gene            complement(26544..27917)
                     /locus_tag="PK839_01330"
     CDS             complement(26544..27917)
                     /locus_tag="PK839_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG71405.1"
                     /translation="MKTKNYIPMLALLGFTTLSCSSSLQVSNTSSWEDEIYRANTKTS
                     KTELAQDSKDSTPVQTNLNDYAQLDKKYADELAVNQETTQNDTISSDKENVNPYESIL
                     SDSYQESYERRLKGMEDPMYGLNDWSVYYSDSYRYAQAYDPNYYRLVVMGSQVWVEPW
                     YIYNSFSWPRTSFYFGFGYGWGYNPWSWYYDPFFYSPFNYYNPYYYSAWNFPGNYWIN
                     HGIEYSYLNSGNFYYGRRPGESSFNTSSRRTASGVTPTSSQAVTRQRGDVQSSSINPI
                     QSTKNTQTHVTPTYKNNRGSVEGTQRSGEQITAPRRETGTTLSNPSSTRGSAVSSGTR
                     SEKPTRSIGISEPTGRAYNPGYDKPRTSSSGTQSIQRPSTGRGTTTSPSGRSSSSPTY
                     NSPGRVSAPSNGSATRSGNSVQSKPEGSRSSGNSSGTYTPRTESSSSGSRGNSNSGSS
                     GAKTRGR"
     gene            28016..29491
                     /gene="proS"
                     /locus_tag="PK839_01335"
     CDS             28016..29491
                     /gene="proS"
                     /locus_tag="PK839_01335"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010262587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HOG71406.1"
                     /translation="MAKEITSRNENYSQWYNDIVIKAGLADYSAVRGCMIIKPYGYAV
                     WEKIQAQLDRMFKETGHQNAYFPLFIPKSFFSREASHVEGFAKECAVVTHYRLKNDAS
                     GMGIVVDEDAKLEEELIVRPTSETIIWNTYKSWIQSYRDLPILINQWANVVRWEMRTR
                     LFLRTTEFLWQEGHTAHATRQEAVEEAERMINVYAQFAREWMAMPVIMGRKSEAERFA
                     GAVDTYSIEALMQDGKALQAGTSHFLGQNFAKAFDVKFLNSEGALDYVWATSWGVSTR
                     LIGALIMAHSDDNGLVLPPKLAPTQVVFVPIYKSREELDRISSVVSQLRNELHAVGVT
                     THYDDRDNYKPGFKFAEYEMKGFPIRIAIGPKDLENGTVEVARRDTLQKQIVSSNSLG
                     KFIIGLLDDIQNNLFSKALEFQRQNTTVVNSYDEFKSVIAGKGGFVLAHWDGTPETEA
                     IIKDETKATIRCIPFDSKPEEGKCIVTGKPSMQRALFAQAY"
     gene            29548..30255
                     /locus_tag="PK839_01340"
     CDS             29548..30255
                     /locus_tag="PK839_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009599067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG71407.1"
                     /translation="MAEEQQPTKEQIFDLLNGKSVLKQKIYDNTFEVLGKIKLVLRDL
                     ANEYNANLKNTDRRVRLEYRDRGKFEAEIRVAGDLLIFSMHTNVFEFDRDHAIWKLSY
                     VKDDKFASYCGVINIYNFLVDSFRYNRIDDLGYLVGRIFINRDFHYFVEGKRQMGFLY
                     NNFGTAVVDMKSLRQIVETAIMYSLEFDLLVPPYDLVKIASVQQMNSKIESSNMQTGK
                     RLGFKFNSDDVLEEENK"
     gene            30284..31915
                     /locus_tag="PK839_01345"
     CDS             30284..31915
                     /locus_tag="PK839_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007558685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HOG71408.1"
                     /translation="MNRLCITFFAALMALSSCGTKHNKEVKSEMQKKLDEFAPVELRS
                     DITHLSTREIQMLPYLFDAAQIMDDLFWLQAFGSKEEFLDTITDKATFEFALVNYGPW
                     ERLNDNKPFIQGFGEKPLGAQFYPPDMTIEEFEAFSSPDKTSQYTLIRRNSDGKLESV
                     PYHITYKDRIDKAAELMVQAANLAEDAGLKRYLNLRAKALQTDEYFESDMAWMNMKNN
                     NIDFVVGPIENYEDALFNYKAAYEAYILIKDLEWSQKISHYAALLPKLQESLPVDPAY
                     KKEVPGLDSDLGVYDVVFYAGDCNAGSKTIAINLPNDPKVQLKKGSRKLQLKNAMRYK
                     FDKILLPIGNLLIDEEQREHIKFDAFFENVMFHEVAHGLGIKNRVDGKGGVQESLKEM
                     HSALEEGKADILGLYMVSQLAAMGELPDKDMMDNYVTFVAGIFRSVRFGAASSHGKAN
                     MVCFYYLKERGAFERNPETGTYRVNFDKMTEAMNSLAHDILILQGNGDYEGVKKLFAD
                     KGFIAEELQHDLDRVSEASIPRDIRCIQGKEIFGL"
     gene            32014..32583
                     /locus_tag="PK839_01350"
     CDS             32014..32583
                     /locus_tag="PK839_01350"
                     /inference="COORDINATES: protein motif:HMM:NF038262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SiaB family protein kinase"
                     /protein_id="HOG71409.1"
                     /translation="MNLTFNFIKSVSNIYDEMIDNGFSLVYLGEFSHEITKMFTSMAE
                     SDMEKNSEERSVQRKVYHVMVETLQNMNKHSDEIKERNIGNGLFIIGKKHDTYYVITS
                     NKVARKHKDHLEQSLITVNNASPEELKEMYKRQIREGTLSEKGGAGLGLIDIARKTGE
                     PLNYQFLQLDEDNYFFILKVEINARKFYK"
     gene            32624..34528
                     /locus_tag="PK839_01355"
     CDS             32624..34528
                     /locus_tag="PK839_01355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG71410.1"
                     /translation="MSLKKSFLQLTLRLGLAITVHLSFLLFTSTSFALTTSKFDSVQL
                     FWNPDTLSRSDTNLFNRRGNEVLKSNNAIVNNLLDLIYCEEEDTRQKEIYAEQAKAYQ
                     KFSRYAGRIIADIQIVRLKPFGEYINDTTLKPENSLQKFGNSLHSLTLRRSVENNLLF
                     SEGQELDPVDLVETEQLLRNLLYIEDVGINIEPLQDTNYVKATVVTKDKWTLNMGVRI
                     ESSAKGRITLSERNFAGIGIGATGRVYYDEEQKEKWGYMGELNIPNIAGTFITSDLWF
                     REGVGYKSYCVDVKRDFYASKTKYALGGTYVKSQEPYKIFPVDSIVSIDYSLMSFWVG
                     RSFRVGKPVIIASPYIFTLALKYRQVDFYKSLPTSMNLNPYFHSNYQVLLAAGFSNQN
                     FAQDNFIYSFGATEDIPVGFIVQLIGGVEDSEYQRRLVAGGEISAAEITPAGYLFIAA
                     RTGGYMAEGPRAEQGMINFKSTFISNKFTLGKYFGRQHVGVSFTRGFSRFEGEREYLA
                     LNKDNGITGLRSNSLVGQTRLIVNFETTLFSPHLYYGFRPSCFAFCDLGMVGGSNEVI
                     FGNSLFSGFGLGFKVKNESLIFPSFIFRLGYYPNVPQNADVAYWIITSQDRKKFEQFR
                     MKEPALLPFE"
     gene            34687..35322
                     /locus_tag="PK839_01360"
     CDS             34687..35322
                     /locus_tag="PK839_01360"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HOG71411.1"
                     /translation="MGVLVGQKAPVFKASAVIKGHEIVENFSLEQFIGKKYVVFFFYP
                     ADFTFVCPTELLAFQDKLEEFEKRNTQVVACSVDSEFSHWKWLQTEQKDGGIKGVTYP
                     IVSDLSKTISENFDVLAGHYDYDDNNGDAVFVGVPQAFRGLFLIDKSGVVRHQVVNDM
                     PLGRNVDEVIRMVDALQYFEEHGEVCPANWKKGEKAMKATQDGVSDYLSHR"
     gene            35417..36745
                     /locus_tag="PK839_01365"
     CDS             35417..36745
                     /locus_tag="PK839_01365"
                     /inference="COORDINATES: protein motif:HMM:NF012640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HOG71412.1"
                     /translation="MRKRLTYLVENPEVVTDNMAKALLSWDVGIVLRGKDAMPVPGTD
                     MVSQFDCAAGFGVAASCKPADNFFKNFEYFTDANNDWILGFLGYDLKNELENLQSGNF
                     DGLAFPEMFFFVPEVFMSIADHNLCVYYYPGKTSDLTVSKLVDDLLRVELVANPQQPT
                     ITFKSRYSRYEYLTQVQRIKNHIGRGDIYEMNFCQEFYTEGVSIQPARVFNRLYSNSP
                     TPFGAFLKIGEKFLMAASPERYLRKNGNVVVSQPIKGTAPRMALPDMDSQVVEKLKSD
                     AKEISENVMIVDLVRNDLSRVAVPGSVKVNELCGIYTFRQVHQMISTVSCIVPEKLNL
                     VEPIKATFPMGSMTGAPKVRAMQLIEEFEKSRRGIFSGAVGYITPNKNFDFNVVIRSL
                     LYCGQRKYLSFSVGGAITAKSDPENEYNECLLKAKAILQTLNARIENVAG"
     gene            36732..38051
                     /gene="tilS"
                     /locus_tag="PK839_01370"
     CDS             36732..38051
                     /gene="tilS"
                     /locus_tag="PK839_01370"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HOG71413.1"
                     /translation="MLQDKFINFIRVHRLADENDSILLGVSGGIDSMVMLHLFQKTGF
                     KFAVAHCNFGLRGEESDGDEEFTRTYCREKGVVFHTTKFSTVQYATDKSVSIQVAARE
                     LRYDYFNELCREHGYNKIAIAHNMNDSAETVLLNLIRGAGIRGLTGIRPVHGNIIRPL
                     LFATRPEIENYSKQLGISYREDSSNATLKYKRNFIRHRVIPLLQELNPSAVETIYDSS
                     YHVNEAISLVDRQLDEIRRKVVSFRKDAVLFSVEELKREPAARIFLVEELRHYGFSGN
                     MATQVFDLLESTPGKYVDSPTHTLLRDRKYLILSAKISTSNACIEVGEDCSRIEKPFP
                     MRFERIVEVKIDSSPQVAILNYSKLRFPLTLRCWQPGDRFIPLGMKGYKKVSDYLVDC
                     KVPLNQKNEVYVLLSNNEIAWVVGYRIDDRYKISTETAEALKISVLL"
     gene            complement(38143..38598)
                     /locus_tag="PK839_01375"
     CDS             complement(38143..38598)
                     /locus_tag="PK839_01375"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG71414.1"
                     /translation="MKRLTLLLLLVPFLSGAQNNVTEKNGLYLDSNGKPFTGTYQEYF
                     ENGQVKSQMEIGQGLLNGQVKVYFPSGELNEIRSYIKGEKSGRWETWNAQGVKVAEAN
                     FLNGKKNGKWYVWDEKGTLRYDMTYRMGEKSGTWIIYNDKGLETSRKEY"
     assembly_gap    38627..38726
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(39043..40404)
                     /locus_tag="PK839_01380"
     CDS             complement(39043..40404)
                     /locus_tag="PK839_01380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG71415.1"
                     /translation="MKKMSLLTSTKGLAFAAILLGLGMASCNDDEDEPKPPTGQKVTV
                     SGDITANTTWSANDTIMLEGFVYVNNGVTLTIEPGALIKGLSDTKAALIVERGGKIMA
                     NGTQDRPVVFTSDKPAGQRKPGDWAGIIICGKAPINITGGEAEIEGGTGAIYGGTDPA
                     DNSGSLQYVRIEFAGYEVSSGNEINGLTLGGVGSGTVIEHVQVSFGRDDSFEWFGGTV
                     NAKYLVSYRGGDDDFDTDNGFSGNVQYGLILRGPEEADKSDASNGFESDNDANGSSNT
                     PLTSAKFSNITILGPYATKTQPNVNPNHKHALRLRRNTRLNLYNSILAGFATGPRIEG
                     PSMALYNTTGGPEIKNCIMAGMIKDFQVNTDGTTLTQEQYDAMWTAGNNQTFAENADI
                     KLTNAWSWGNVNPMPQSGSPVLTGASFSGLSGFETVAFIGGFGTTNWTSGWCSWDPQN
                     ARY"
     gene            complement(40597..40962)
                     /locus_tag="PK839_01385"
     CDS             complement(40597..40962)
                     /locus_tag="PK839_01385"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOG71416.1"
                     /translation="MKRILVLLIAALTLNVFGATTDDKTKGESTTSLPVKSITINGKV
                     TDKHTGEALAGVAVKTDGSTTCYSDFDGNFSIQVNPEKLENLSAELISYSTSVVKVDI
                     NKTINPTLKIKIELEPIAE"
     gene            complement(41072..43885)
                     /locus_tag="PK839_01390"
     CDS             complement(41072..43885)
                     /locus_tag="PK839_01390"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOG71417.1"
                     /translation="MRTNNRRIAYLFTLLCFLLATSASAQTGSIVGTVVDMGKDETLV
                     GASVIIEGTFKGTITDFDGKFKLENLEPGIYSVQASFISYKPTILDGIRVEANKTTTV
                     SIELEEITTELEGVTVTATRKTSTNLAMISSIKASLMVATGISAQQINNSQDKDASEV
                     IRRVPGASIIDDRFVVVRGLNQRYNSVWINNASTPSSETDQKAFSFDVIPSAMIDNMM
                     VSKSMSPELPADFTGGFIKIFTKNMPESNFMSISYTTGYNTRAHYGDFQLITTGKTDW
                     LGVDGGTRNLPSGFPNSLKGYPDAELADLGKRLNTSWAPKGVSAFPEQKLNFSFGRKI
                     QKGNRLLGNITSISYSHTYSSDELVNKGYQVQLNPGEIPPYSYNYTDSTYSRSVRLGI
                     LHNWSLFPGKGTKIEFRNLFNLMGNNTAVIRNGLNGYEGYTIRSYQDKYMSRITYSGQ
                     LGGEHKLGSNSDNTLDWVLGFAYSGRNEPDLKQLRTTLQSEPLLPHYNEYYASVGLTP
                     SVSDAGRLFMNLHEYIATAGTNYEAKIKIGSWKPVIKTGLYSEYKNRTFDARILGFAK
                     NVSYTESVWLPVETIFSLENINTTPSGFILKEMTSRSDSYDATNLLLAGYLTINTNIT
                     QKLRFYGGARFEMNNMRLNSYDMYNKPVDVKLDHIDIFPSANLTYNFNEKNLLRLAGG
                     LSVNRPEFREIAPFYFYNFDEEADYVGNVNLKNAYVWNFDFRYELYPNAGEIISIGIF
                     YKHFTSSIESVFRPAGNRLTFTYDNADNAQSAGAEVELRKSLEFIPLLKDFSAVLNAS
                     YIYSRVNFPANSIFRDRPMQGQSPYLVNAAVFYSNTKHKLNLSLQYNLIGDRIIAVGL
                     PFQNSNQDIPDITEKHSHLVDFTASKGIGSKMEIKAGIKNLLNQKSIKYQAFKQADGG
                     DLNLSSREMQPGITFSLGFSLNF"
     gene            44076..44768
                     /locus_tag="PK839_01395"
     CDS             44076..44768
                     /locus_tag="PK839_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOG71418.1"
                     /translation="MSKPGYRILIVDDEPDIIEFLSYNLTKEGYEVDTATNGRRAIEK
                     ATEFSPDLILLDVMMPDMDGIETCQELRANPLTKDCLIAFLTARSEDYSQIAGFDAGG
                     DDYISKPIKPKVLISRIKALLKRSGSLEEAPEPTPEIPEEGIYINRERYLVYLDGREL
                     VLPKKEFELLALLQSKPQKVFTRDEIYQAIWGDDIVVGDRTIDVHIRKLREKLGDQYI
                     RTVKGVGYKFTV"
     gene            44834..45241
                     /locus_tag="PK839_01400"
     CDS             44834..45241
                     /locus_tag="PK839_01400"
                     /inference="COORDINATES: protein motif:HMM:NF033749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HOG71419.1"
                     /translation="MELFSWKNDYSVKVGSIDSDHQKLFDIINSLFTAMSKGEGRQVI
                     SGLVKELQSYTIYHFNREETFFRVTSYPNAANHLEQHKIFVSKVKEYKEKIDKGNNDF
                     SPEMLTYLKDWLLNHILVTDKAYSDHFTKNGIV"
     gene            45467..45874
                     /locus_tag="PK839_01405"
     CDS             45467..45874
                     /locus_tag="PK839_01405"
                     /inference="COORDINATES: protein motif:HMM:NF033749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HOG71420.1"
                     /translation="MELISWESNYSVKVGSIDADHKKLVEILNSLFMAMSKGEGSKII
                     SDLVKELQRYTIYHFSREESFFRITGYPNTQKHIEEHKQFIGKVKEYKEKIDKGNNNF
                     SSEMLAYLKDWLLNHILVTDKAYSEHFVKHGIA"
     gene            complement(45974..47587)
                     /locus_tag="PK839_01410"
     CDS             complement(45974..47587)
                     /locus_tag="PK839_01410"
                     /inference="COORDINATES: protein motif:HMM:NF024500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OstA-like protein"
                     /protein_id="HOG71421.1"
                     /translation="MQKASTSTTRIFLFILGFTSLHPTFSAAQQKTTIKLEGAKAMKN
                     LNLGDQKIVRYIGNVRFFYDNTRVRCDSAYYSQNENWFDAFNGVVVSQGSSNIYGDKL
                     HYDGRTSEGRLTGKEVKMVDKDFTLVTDALDFNSKTSLAFYTTGGVATSNDSSTLTSI
                     KGYYNNNDNLISFANKVVLKSPDGNAFTDSLSYHTKNEVAYFFGPTFIYDGENFIYCE
                     KGWHNRKLQQSNFQNNAYLISNSQKLFGENIYYDKQNGYARGIGNVVMVDTVNRTYIY
                     GQKASYWQEKEEAEITEQPYMMMIDDSDSLFLRTDKLLMITAKTGDNQKADSSYRLVK
                     AIGRVMYYRHDIQGVCDSMIYNTRDSVLEMYGNPVMWNEANQMTADFIRIHISNKKIS
                     QADFLGNAFISSREDSAYFSQMRGKTILARFSDGQLTRMDITGNGQVVYFVRDKGQIT
                     MVNRAEGSNVDVHIKNNKVSRVVFKQKPTSNLIPIEQVEPEDMTLKGFKWLGQLRPES
                     KYSVIPTSLTLVPLEKGTQRRMEMVANFK"
     gene            complement(47600..48949)
                     /locus_tag="PK839_01415"
     CDS             complement(47600..48949)
                     /locus_tag="PK839_01415"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOG71422.1"
                     /translation="MKPARLAITALLLQLFIVNAYSQEAQVIDQVVAVVGGEHILLSD
                     IEQELLRQKMEGSPTGENNRCNVLEQILIQKLLLTQAKIDSLKTNESSVEMEIDRRLK
                     YFTSQLGSEKALESYFNKPIYEVKNDLREAIGEQLLTQQMRQKIVDKVKITPAEVKSF
                     YKNIPTDSLPTVPEQYELQQIMIYPPSTEEAKLQVREKLLEIRNRILQGERFSTLAVA
                     YSEDRASAIRGGELGFRYREELVKAFADAAFSLKEGQISQIVETEYGFHIIQLIEKRD
                     NQVNVRHILMKPQFSGDMLAQAMSKLDSIATLIKADSLTFEAAAMRFSQDKKSFLNGG
                     LVINSYNNTSLFEKEQLQPADFYAIRELKEGEMSSPFESRDEHANVVFKIIKVKRIIP
                     PHKANLKEDYAIIQNIAKVSMENEILQEWLDKKIQSTYIRVDEPFRNCSFRSKGWIK"
     gene            complement(48988..49845)
                     /locus_tag="PK839_01420"
     CDS             complement(48988..49845)
                     /locus_tag="PK839_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG71423.1"
                     /translation="MKSFRLVTFSLVGLTVVLASCFNSGKKPMPLARVYNSYLYPSDI
                     ANLIVGNVSKEDSALIVDAYIDKWIQTQLMLKIAENNLSDSQKNVTRQLEDYRTSLLI
                     FKYEQAYLNQKLDTVIKMADLKAYYDDNKDNFVLNETIVKALYIKLRREAPQIKKIRE
                     LYLSNSDEDIKQLDNIAYQAATKYDYFNDKWIPLSFILRELPFPIANPQREVFEKRYV
                     EMEDGDFVYLVRFMDAIPSGSTAPFDYITDDLKQIVLNARKQKLISELEQRIYSKGQD
                     DENFQVYKR"
     gene            complement(49854..51839)
                     /locus_tag="PK839_01425"
     CDS             complement(49854..51839)
                     /locus_tag="PK839_01425"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOG71424.1"
                     /translation="MNKIIRNTLAGFMVITCGIAAVAQPTEQKILLTINNEPVTAGEF
                     QGLYLKSTSIENAKPTREVLEDYLNLFINYKLKVKAAKDNGIDTTAAFKNEYQGYVEQ
                     LAQSYLIDNQVLNNLIEEAYNRMQYEVSASHILISIPENASPSDTLKCFQKAMEARSR
                     ILKGETFGSVAVAMSNDPSVSRNSGFLGYFTAFQMVYPFESAAYSTPVDSISMPVRTR
                     FGYHIIKVHDKRPARGQVKVAHIMIRVPPNPSPQAAEDARLKILGIYQRLTAGADFAQ
                     LAKDESQDPSTSKSGGELPWIRPGQVIPEFEAVAFSLQHDGEISQPFQSSAGWHIVKR
                     LAKKELGNREELIPEIRAMLMRDSRGMLPRTAFIENLKKEYSFKQDTSKLKLLIPLLD
                     SSLYKGTWKSPNLKSNSTLISFNGNELKLSDFCNFIEQNQGLIGKHSIPAFLMNAYTE
                     WVNLTLTSYEKSKLPDKYPEFAQLTREYYEGMLLFEISNREVWQKSSDSLGIANFYNE
                     HQDSYKWGKRIHFAVYTLTDPKIKKAFVSGIEKRSKKGLTPLDYANRFNSPAKQVATV
                     VEKSGDPTLPEVADYNSWPKKFSVRDTQNGEVVVVEVLDTTIGDVKPLTDCKGEVISD
                     YQNSLEREWLEKLKKHYTVIVNRSVFDEISAWQNNNK"
     gene            complement(52026..53507)
                     /gene="guaB"
                     /locus_tag="PK839_01430"
     CDS             complement(52026..53507)
                     /gene="guaB"
                     /locus_tag="PK839_01430"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HOG71425.1"
                     /translation="MSFQSEKVIAEGLTFDDVLLVPAYSEVLPRNVNLSTKFSRNIDL
                     MAPIVSAAMDTVTESTLAIAIAREGGIGVIHKNMSIEAQARQVREVKRAENGMIFEPI
                     TISPDATIGDALTLMRENHIGGIPVIDSLGFLIGIVTNRDLRFHEDVSTPIRQVMTSD
                     NLITTSKSIDLENATEILKKYKIEKLPVVDENNKLVGLLTYKDITKVKDNPNACKDKM
                     GRLRVAAGVGVTRDTLERVRALCEANVDAIVIDTAHGHSKGVVDMLKLVKREFPNIDV
                     VVGNIATAEAAKMLAGAGADAVKVGIGPGSICTTRIIAGVGVPQLNAIWDVSHALEGS
                     GIPVIADGGIRYSGDIVKALAAGADSIMTGSLFAGVEESPGETIIYNGRKFKSYRGMG
                     SIDAMQQGSKDRYFQDVEDDIKKLVPEGIVARVPYKGTLSEVFYQLVGGLRAGMGYCG
                     TTSIEMLKKARFVKITHAGIIESHPHDVAITREAPNYSRDTDY"
     gene            53664..55673
                     /locus_tag="PK839_01435"
     CDS             53664..55673
                     /locus_tag="PK839_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002562571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HOG71426.1"
                     /translation="MTLIFDLQVIDRLFAKFAPADFTTLDRFEEILKQYWGYNIFRSI
                     QRDIIESVYNGNDTLALMPTGGGKSITFQVPALALEGTCLVITPLIALMKDQVENLKK
                     RDIRAVAVHSGLTRHEVDVALDNCAYGDVKFLYLSPERTTTELFRERLRKIKVSMVAV
                     DESHCISQWGYDFRPSYLRIAELREIISEPVPFLALTATATPEVVEDIQQKLKFRNGK
                     VFSMSFARENLVYLVRNVEDKPKHLLKITHGIPGSGIVYVRSRSKTREIAQMLQKEGI
                     SADFYHAGLTMEVRNAKQNDWQVGKTRVIVATNAFGMGIDKPDVRFVVHVDLPDSPEA
                     YFQEAGRAGRDQKLAYAVLLFNNADSLKIEQRVESNFPPIEEIKRTYQALGSYLNVAI
                     GAGKGETYDFNLMDFCSAYKLSSVRAYSSLKILEREGYLELTDELDNPSRIMFLAGKQ
                     DLYKFQVAHAELDGFIKAILRNFTGVFSQYVRIDEVYLARLAGITVDKVIENLKTLGR
                     YKIISYIPKKRTPLLVLVEERLDDKNLRIEPSSYRLLRSRFEDRVKAMLSYANNQSKC
                     RSQMLSEYFGQKDSYRCGKCDVCTSRNELDMSKYEFDMILDGLKRRLQAGPCSEEELI
                     DAIKRNPDRVARVIKYLMDTGKIFHNEKGMLAWHHRKEGGFEVEE"
     assembly_gap    55929..56028
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            56088..58196
                     /gene="recG"
                     /locus_tag="PK839_01440"
     CDS             56088..58196
                     /gene="recG"
                     /locus_tag="PK839_01440"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HOG71427.1"
                     /translation="MADLILNDIKFLPGVGPKRAELLQKELNISTFGDLLYFFPFRHI
                     DRSKIYKISEINEDLPYIQIVGRILRIEEVGVGRNRRLTAYLSDGSGSVELVWFRGVS
                     WMAKWLKPNIEYLVFGKANVFGGHFSIAHPEMEEYKTGQEKIMGTLQPVYSTTEKLKS
                     NRITSKTILGLQQTLIQAVNGKIEETLPPYLIEQQKLMTLPEALRNIHFPQSVLLLRK
                     AEHRLKFEELFFIQLDILRKQTIRQSKINGHLFSRVGEMFNRFYSERLPFPLTNAQKR
                     VIREIRRDMGSGKQMNRLLQGDVGSGKTIVALMSMLIAVDNGFQACLMAPTEILANQH
                     FDSITSLLGDLPVTVGLLTGSTRQSERNTLHKSLEAGELQIIIGTHALIEDVVKFKNL
                     GFVVIDEQQRFGVAQRAKLWEKNNNPPHVLVMTATPIPRTLAMTVYGDLDVSVIDELP
                     PGRKPVKTLYYTDLKRNVVFGLMRQQLKEGRQVYVVYPLIKESEKMENLKDLQDGYES
                     IVRAFPPPEYVTVVVHGQMKPEEKESSMDIFVKGKANIMVATTVIEVGVNVPNATVMV
                     IESAERFGLSQLHQLRGRVGRGADESYCILVSSLKISSESRKRLQIMVNTSNGFEIAE
                     MDLKLRGPGDIEGTLQSGIPFDLKIANLSTDGNLLQHARAVARDVLNDDPLLEHPRNA
                     LLSQRLDELANSRSNFGRIS"
     gene            58364..59197
                     /locus_tag="PK839_01445"
     CDS             58364..59197
                     /locus_tag="PK839_01445"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HOG71428.1"
                     /translation="MKHKPIIHVVFTLLLLVVSEATLSQSVNPCGIVNRSFASGEKLV
                     FEISYTWLFVWTDVGMAELTVSAEKRFGRDLLHIKASGKTHTFYNWFFEVHDLYETWV
                     DPVTVEPVYFNRDIYEGGFTKENEYFYDWNKNQVAIRVRRKKGPNRYDTLSVSRCTYD
                     VISAVYAARNLDFSGIKPGKTFPVEVLMDEEVYHIGYRFLGREEKRVSGLGRFRCLKF
                     QIDVVAGDIFSGDQKLYLWVTDDANKLPVAVETPIQVGSIKARLNTYEGLRHPLSSKI
                     P"
     gene            59258..59668
                     /locus_tag="PK839_01450"
     CDS             59258..59668
                     /locus_tag="PK839_01450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG71429.1"
                     /translation="MSRAISILITFLLLFTIESCLDNENTVRYTDVIQMEAGPVPDTM
                     VVNETYSIQFRMGVPNSCWHSLALNQEEFNDSTYRFWATAVYENHGENCAQVVVTKDT
                     VIAFKPTLAKPHILVFFNDPASDPRVDTVVVTPN"
     gene            59880..60821
                     /locus_tag="PK839_01455"
     CDS             59880..60821
                     /locus_tag="PK839_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOG71430.1"
                     /translation="MKKILVTGGAGFIGSHLCERLLNEGNEVVCLDNYFSGKKENVVA
                     LMSNPYFELVRHDVTMPFFAEVDEIYNLACPASPIYYQYNPIKTVKTSVMGAINMLGL
                     AKRVKAKILQASTSEVYGDPLVHPQPETYWGNVNPIGARSSYDEGKRCAETLFVNYHS
                     QNGVKIKIARIFNTYGPRMSVNDGRVISNFIVQALRNEDITIYGDGKQTRSFMYVDDL
                     VDALIRLMDSPDEFTGPVNLGNPCEYSMLELASKVIELTKSRSKLVYFPLPEDDPLQR
                     KPDISLAKKFLGGWEPKVMLEDGLKKTIDSFEHILIR"
     gene            60826..61701
                     /gene="rfbA"
                     /locus_tag="PK839_01460"
     CDS             60826..61701
                     /gene="rfbA"
                     /locus_tag="PK839_01460"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005863000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HOG71431.1"
                     /translation="MKGIILAGGAGTRLYPITKSISKQIIPIYDKPMIYYPLSVLMLA
                     QIRDILIISTPKDLHLYQDLLGDGAQLGISISYAGQPSPDGLAQAFIIGENFIGSDSV
                     CLVLGDNIFYGGNLTEMLLESAKLQDGAIVFGYNVKDPQRYGVVGFDSDGKVNSLEEK
                     PTNPKSNYAVTGLYFYSNDVVAKAKGLKPSKRGELEITDLNRLYLEEGRLKVKLMGRG
                     MAWLDTGTHESLLQASIFIQTIEERQGLKIACLEEIAYRNGYINLEQLRKLATPLENN
                     QYGEYLKRLASGNFS"
     gene            61741..62310
                     /gene="rfbC"
                     /locus_tag="PK839_01465"
     CDS             61741..62310
                     /gene="rfbC"
                     /locus_tag="PK839_01465"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007653212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HOG71432.1"
                     /translation="MLTVEKTSIPDLLILKPNIFEDSRGYFFESFNAKLFDDFAITSN
                     FVQDNESFSKYGVIRGLHYQLSPYSQTKLVRVVYGKILDIALDIRRNSPTFGRYVALE
                     ISAENKAQFYIPKGFAHGFSVLSDSAIVCYKCDAYYSKEAERGIRFNDPGLKIDWGIP
                     DGSMVVSEKDLKLPFFSEADINFNYGSIT"
     gene            62294..63166
                     /gene="rfbD"
                     /locus_tag="PK839_01470"
     CDS             62294..63166
                     /gene="rfbD"
                     /locus_tag="PK839_01470"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007569087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HOG71433.1"
                     /translation="MAASPSRVLVTGANGQLGTALRKRATKPANHLVATFTDFNELDI
                     TSDQQVDNFFQQNHFDYLVNCAAYTAVDRAEDEPEKAFLINGYAPGLLAEACKSHGTK
                     LFHISTDYVFDGMSNSPYHESSPVNPLSVYGKSKLMGENKVLESGVGMVIRTSWLYST
                     TGNNFVKTILRNASSKAQLNVVFDQVGTPTLADDLAAVLLQIIEKGDSSFRPEIFHYS
                     NEGVCSWYDFAYEIVAIAGLPCRINPIETNDYPTRAIRPRYSVLSKEKIRNLLPIEIP
                     YWRDSLKTFLKEML"
     gene            63184..64932
                     /locus_tag="PK839_01475"
     CDS             63184..64932
                     /locus_tag="PK839_01475"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HOG71434.1"
                     /translation="MNADSSDSQVMERAKQWLSPVFDMETREQVQQLISGNHDELVES
                     FYRDLEFGTGGLRGIMGVGTNRMNIYTVGIATQGLANYLKKQLPGIPEVRAAIAFDSR
                     NNSIKFAQIAAKVLSANGVRVYLFDSLRPTPELSFAIRTLKCHCGIVVTASHNPKEYN
                     GYKVYWEDGAQVVPPHDSGIIAEVQAIRNISEVNFNGNESLISTIGPEMDEIYSNTLS
                     SLSLSPKIIKKHKEIVIVYTPIHGTGVKLVPKTLRKFGFTNIVNVPEQDVVDGNFPTV
                     SSPNPEEPSAMRLALEKAKEVNADVVMATDPDADRVGLGVRTPEGKYTLLNGNQAASI
                     LIYYVLQKWHEANRIKGREFIVKTIVTTDLLAEIARKYGVECYEVLTGFKYIAEKIRQ
                     LEGEKKFIAGGEESYGYLCGDFVRDKDAVISCALFAEATAWAKEQGKSLYEVLAGIYR
                     EFGLYSEKQLSITLKGKEGLEEISRMMVGFRKNPPLQIDGLPVITIHDYLSQETKDVP
                     SGSRRPLQLPKSDVLQFLTADGTKVSVRPSGTEPKIKFYIAIRGDFNPNAGIDEQLAL
                     LDERISRIAAELKITR"
     gene            64963..65379
                     /locus_tag="PK839_01480"
     CDS             64963..65379
                     /locus_tag="PK839_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOG71435.1"
                     /translation="MKSYRKELWFETRNRRELINITGEVEKCLAESGISEGLALVNAM
                     HITSSVFINDDESGLHHDFEIWLEKLAPEKPYAQYRHNGFEDNADAHLKRTIMGREVV
                     VAITNGKFDFGPWEQIFYGEFDGKRRKRVLVKMIGE"
     gene            complement(65422..65796)
                     /locus_tag="PK839_01485"
     CDS             complement(65422..65796)
                     /locus_tag="PK839_01485"
                     /inference="COORDINATES: protein motif:HMM:NF014248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPP20 family lipoprotein"
                     /protein_id="HOG71436.1"
                     /translation="MKKKLMVILLLTTALAASAQDFVGSAPEWVNNPPKSVRKFYGVG
                     VGTSTTPEMAEEKAKMDAKTAIATQAGAVKVENIKSDKGVTQRKIVNATLSEVKMVKK
                     ACMKDGERYTVWVLMEMKGKRK"
     gene            66037..68673
                     /locus_tag="PK839_01490"
     CDS             66037..68673
                     /locus_tag="PK839_01490"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HOG71437.1"
                     /translation="MDSVRELSAKYSPAGIEDKWYRYWLEKGFFSSTPDEREPYCIVI
                     PPPNVTGVLHMGHMLNNTLQDVLIRRARLMGKNACWVPGTDHASIATEAKVVAKLKAD
                     GIEKSQLSREEFLKHAWEWKEKHGGIILEQLKKLGASCDWSRTRFTMDETLSASVIHV
                     FVDLFNKGYIYRGVRMVNWDPQAKTAVSDEEVVYREVKGKLYFVRYRIEGEDSFVTIA
                     TTRPETILGDTALCVHPDDPRYTHLKGKRVIVPLVNRSVPIIYDDYVDREFGTGALKI
                     TPAHDVNDYLIGQKHNLDSIDIFNDDGTISSAAKLYVGLDRFEVRRRIVDDLESSGNL
                     ERVDDYVNSVGFSERTDAIIEPKLSMQWFLRMKELAEPALKAVVEGDIQLYPPKFVNT
                     YRHWMENVKDWCLSRQLWWGHRIPAWYLPNGGFVVAHNVEEAVELARKAAGNSGLVAD
                     DLRQDEDVLDTWFSSWLWPISVFNGILEPNNPDIRYYYPTNDLVTAPEILFFWVARMI
                     MAGYEYMGDKPFRNVYLTGIVRDHLHRKMSKQLGNSPDPLKLIEKYGADGVRVGMLLC
                     SPAGNDLLFDENLTEQGRNFCNKIWNAFRLVKGWQVDESAVQPQSAIAAIEWFNSLLN
                     KSIEQADDDFAKYRISEALMNLYKLFWDDFASWYLEIIKPAYGKPVDAVTYNATVSIF
                     EKLLIMLHPFMPFITEELWQHIRQRGDGESIMVQQLPHSTGFNQDILIGFDRVKEVVV
                     GVRNIRQENNISPKQPLQLSIRNAAKLDAGLEEIISKLASINSLTYIDQKIEGAATFM
                     VGTTEIYIPLGGLINTEEEREKILSELEYTKGFLEKVMKKLNNERFTSNAPKAVVELE
                     HKKQADALAKIEALQERLKSLG"
     gene            68965..70470
                     /locus_tag="PK839_01495"
     CDS             68965..70470
                     /locus_tag="PK839_01495"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010538104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HOG71438.1"
                     /translation="MNDSEILMNPNELVRFLKKPSAEFTKNDIIRFCEAKNIEMVNFR
                     YVADDGRLKTLNFAISSKEYLDTILSAGERVDGSSLFSFIGAGSSDLYVIPRYRTAFV
                     NPFSEVPAIDILCSFYDSEGKPLESSPENVLRKAHNLFKSETGCTFKALAELEYYVIS
                     ERDDFFPGTDQKGYHSSAPFAKWEDLRKEALICITRAGGMVKYGHAEVGCFMTEDTLY
                     EQHEIEFLPMPVEDAVDQLVIAKWMVRMLGYRYGVDISFAPKITVGKAGSGLHFHLMV
                     EKDGKNLVFENGRISNTSKKMIAGILDAADAITAFGNTIPTSYLRLVPHQEAPTNICW
                     GERNRSVVIRIPLGWVGTEQMVTHANPADTSAQPAGLNRQTFEYRVADCSADPYLTVA
                     ALVVASLNGLRMKDALQLADKLYVDVNIFSPEYKEKLAALKQLPASCWESAEALEAKR
                     HIFEADGIFPKGMIDSRINNLKSFQDKGLSEKIYGKTEEIRQLVERFIHIG"
BASE COUNT        18933 a        15003 c        16646 g        19754 t
ORIGIN      
        1 cagccttgat tttctttggt tctttcttgt atcaagacaa gaaagaacac ttagctggct
       61 cttcgccaca attttgcatt gcttttcggc tcgccggacg atgccttttt tttaaacctt
      121 tttaatgtca ggtgaatccg ccaggaggca tacaagttcc gccggtaggc ggacaagttc
      181 tgccagtgat gccggacaag tttactttag cctttatcct tatttgcacc gtgaaccaac
      241 aagggttgaa agtttgtcct gggataaagt ggaagccagt aggatagagt atgttttacc
      301 atttaatgtt tccttaatcc ctatattttt tctaaacttg tacctctcat tgctaagcca
      361 agcgccatga ttcacgtagc aacggttcac tggaataccg acaggtgggt tgaccttcag
      421 ctcaaccagc ttaaccgtta cattcatcag ccattcaggg tttacgcctt tcttagcgga
      481 gaagcggcca agcagaggtc gaagtatttc tacagctcct gtcaacccat cacggagcat
      541 gccgtaaagc tcaacctgct ggccgaaacc atcatgctgc tggcacaatc ggacgaagac
      601 ccgcttatct ttatcgatgg cgatgccttt ccggtaacac ctcttgatga ttttatcggc
      661 atgcagcttg agcgctaccc tctggctgca gtgcaacgct tggaaaacgt gggcgatatc
      721 cagccccatc cttcattttg cctcaccacg gtcgggtttt ggaaaagcat aagtggagac
      781 tggcttaagg gatacgagtg gactaactcc aggggagtta agcgcaccga cgtgggtggt
      841 aacctcctta aaatactgaa cgatttaggt gttgagtgga aaccgctact gcgaaccggg
      901 cagctgagcg agcaccccct gatgtttggc atatacgaaa atgccattta ccaccacggg
      961 gcggggttta ggcaacccct ctccatgtac gacaggctta aacctacatc gcacgtagtg
     1021 aaaaacaagg ctgttgccgg tggaattgac ctcgtccttg ataagctacc cctgaagaat
     1081 cggtttttcc tacagcgtca cttaggcctg aaaaagaata ttctcaggca gaacgttgag
     1141 ctaagcgagg caatctacca tgattttgct tctatcatat ccaaaggcga agcctacgat
     1201 tttatttcca aatcgaggcg ttttccggtt accggcagct gaatttttta gaatggacag
     1261 aggaagttag cctcttgcca ggtaaacatt cacaatttcg ccgatgaaaa accggtgaaa
     1321 gtcgcttttg gggtagtttt tcctgatgat atccctgtct atgaattgct caggtttcag
     1381 gttatcggca tacagctttc tgcattcaag caccatgcgg gcctgctgaa aggctatacc
     1441 atgcccggtg gttgccggta ccaatcctgt ttcctttggc ttgtcgaact ttcggcccga
     1501 ttttgccccg caaaagttga gtgccttgcg ccatttttca tcaaaaaagc taagggtgta
     1561 gtagctgtgt tgctcaacaa actggaaggt gtagcgttgg ggtcggataa aaacctgtgc
     1621 aattggcata ttccatagta tgccgaagct accccacgaa gccgtcatgg tgttaaattt
     1681 ctgctcgtcg cctgccgtga ttagcatcca gccgttatca acagcatcga agaagttatc
     1741 cttcagctcg tagggtgata ccggaacaaa atcctgaagt gtgtccatgc aatccgaatt
     1801 aaatatcaac tcccaaatac ttgcgaataa ggaatcctat tccaaaggtg agggcagcta
     1861 ctcccaggct gattatggcc atttcgctga agcgcctttt gaaattcaaa tccttagcca
     1921 ccgatatgta gtagttgaac accagtataa tcaggatggc aaaggttagg gtaagggcaa
     1981 gcgcagcgaa gtaggattgt agtatgaggt agggagctat caggatgata accgttatga
     2041 cgtatgcaat gccggtgtaa attgatgatt tcagggcatt ttggccgcta ttctcagttg
     2101 ataccgagat gtactgtgag gccgccattg atagcgaggc agcaataccg gttatcaatc
     2161 cggcaatggc aattagcctg gtgttctgca tggcaaagct gaatccggca agcgccccgg
     2221 tcagctcaac caaggcatcg tttaggccca gcacgatgga gcctgcgtag tcgagcttct
     2281 cctccttaat catctcgatt agctcgcttt cgtgttcctc ttctgccctg agaatgccgt
     2341 caatttcggg aatgtagggt agaagctggg ggtaaaccct ttgagcctta tcctcctggc
     2401 gctccatgag cttaatgccg aatgttagtc caaaaaggtg ggtgagggtg aagtagaacc
     2461 aggtggttag cctgttgtgt cctacctccc tgcccgaaat gctcttgaaa aagttgtagt
     2521 ggctgtactc ctcagcggca attcgggata ggatttcccg attcgaagca tccttttcta
     2581 ttttggccag cttgctgtaa atcttaaatc cgtttatttc atcccgttgt actttcaaaa
     2641 tgaggttgct aaactcctcc ttgctcaacg tttcattcat agtgcattag gtttgagaaa
     2701 tattttcgtt taaaagggat ttcaaccctc aacaatagta tgaatcaatt cttgatgtaa
     2761 aagtagggta aaattagcta ttttctgaag tgacagcacg accggcaaaa aaaagtctga
     2821 gccgaagctc agacttttct atttgtgtag tatgaatttg cctgggcggt aggggtaacc
     2881 tcaatatcgt tgatgcaaac atgaggtgga cgtgtaagcg caaagtagat aacctctgcg
     2941 atatcctcgg ccgaaagagg ggtgagaccg gcataggtgt cctttgcctt tttcttatcg
     3001 cccttaaacc gcacgagcga gaactcggtt tccagaaggc ccggcctaac ctcggttacc
     3061 ctgatgttat gctttagcat atcctgcctc atggactgtg agagggcgtg catggcgtgc
     3121 ttggtagcgc agtaaacatt gccattctcg taaacctggg ttccggccgt ggagccaatg
     3181 ttgacaatgt gtccacctcc ccgttcaatc atcaaaggcg ctactttgcg ggtgatgtac
     3241 agaacaccct tgatgttggt atcgatcatc tgctcccagt cctccaccat gccatcctgc
     3301 aggtgattca accccagcgc cagaccggca ttgttaacca gcacgtcaat ctttcgccag
     3361 tgctccggca ggctgtcaat tgcgctatca acctgctccc tgaagcgaat gtcaaaattc
     3421 aggatgtgca cctccttttt aaacttcacc tcaacttcct ttttcagctg ttccagctgg
     3481 gtaagccggc gcccggttac tatcaggttg aagttgtcct gggctagtcg tagggctgtt
     3541 gcacggccaa ttcctgaggt tgctcctgtg attaatgcta ttttcatccg tacaaaattt
     3601 tagtattatt aattagcaat caattagctg cgacctggta ttctacactc gttaacgttt
     3661 ggataaaacc tcaatggcag caggtaaaca aacccctaat tgtttagctt agcaaagctc
     3721 gcaattgacg ctcgcttaat cctctttttt aaccaggcta tcctataggg gtattccatt
     3781 gttatgttgt aatgctttgc atatcaatat gtagacacaa ttttatgtaa aatttaagcg
     3841 gtgtcaaacg tactgtaaaa ttttgtattg caggctgctt ttcgtcattt tttacctatt
     3901 catgttcggt ggatacagcg tttaaaaggt aaatggtttg gtttatatgg aggaagtaat
     3961 gtctggttgg ctgagtctgg gttaaaagta tatacaagtg gatgttgatg tacagcttga
     4021 ttagaattct ttgctaaaag ccttgtttgt agctgctgtg gttgcagctg cctcgtttat
     4081 cccggtttgg cgcagggcat gaacttccag tgcacttcta cattcggctg cttctctagg
     4141 gggccagact tacaacattt agctgggaag ttttaaagga ttgattgaat gacagaaaaa
     4201 aaataatttt gggaaccact attttttgcg aaacaatttt aacggttgta ttgttaatgc
     4261 ggtaacagtg tggggtgtat tagaaaagtc caaaatttct ggtataagat aataatacat
     4321 ctatacatat tgaaaaatat caatacgtat ttttcgatga atagctaaaa aggaattatc
     4381 tttacatttt tttcaaagga tagaaatata aaataagctt ggctaaatac ataacctgtt
     4441 gctagggaag tttttgatag ttaattaatt gagttgagta agcaatggag taaaattctc
     4501 ctggtatgga atgtgaaact attcagcatt taataacata ctatacatgt caactatagt
     4561 aaaagttcgc cgaggcttga atatccctat ggagggtagg gcagaaaatg tgcttgttcg
     4621 caaaaatatg tctgaaacct atggagttaa acctctggat tttccagggt tgcttccaaa
     4681 attggaagtt caggagggag acgtggtgag ggctggcaca ccgctttttt atgataagta
     4741 ccgtccggag gtcaagtttt catcgccggt tagcggcgag gtggtagaga tacgccgtgg
     4801 cgaaaggcgc cagatactgg aagtagtagt aaaggcgaat ggtagtgtcg agtatcaggt
     4861 gtttggagca gctcaacccg ctgcgcttac acgcaaccag gttgttgaga agttactcgt
     4921 ctctggtgta tggccttaca ttcgccagcg tccctttgga atagtagcaa atcctaacga
     4981 cacccctaag gcgattttta tttcatgttt cgataccgcc ccccttgcac ccgatatgga
     5041 cttctccata cagggtgagg atgagaattt tcaggcggga attgatgcgc tgtcgaagct
     5101 tactcctgga aaaattcacc tggggttaaa tgccgattat cctgctgcca gtgcctttgc
     5161 aaaggcaaat ggggttgagc atcactactt cagaggacct catcctgctg gtaatgtcgg
     5221 ggtgcatata caccatatcg atcctattgg gaagggagat gttgtttggg tggttaaccc
     5281 cctggatgtg gttattatag gccgtttatt tttgaagggc atttatgatg cctccagggt
     5341 aatagccctg gccggctctg aggttaccag tccacgctac tatagggtga tttctggtgc
     5401 tcaaattgat agtattacaa gtggaaatct gaattccaac atcgaactaa ggtacattag
     5461 tggaaacgtt ctaaccggaa caaaggtaga gccgtttggg tatctggggt tttatgataa
     5521 catggttacc gtgattcccg agggtaacca ctacgagttt tttggatgga tgtctcccgg
     5581 ttttaataag ttcagcgctt cgcgtacatt cctttcgcgg ttaaccccat ggagaaaatt
     5641 caaattcgat accaatttac atggtggaaa gagagcctac gttataaccg gacagtacga
     5701 aaaggttatg ccaatggata tccttcctgt tcacctaatt aaggccatcc tggccaacga
     5761 tattgagcgg atggagcagc tgggtattta cgaggtggtt gaggaggata tggcactctg
     5821 tgaatatgtt tgcacctcta aaaccgacgt gcagcagatt cttcgaaagg gcatcgagtc
     5881 gttaattaag gagctaagct agacatttaa ttatacctca aacaatggga ttacgaagct
     5941 acattgataa aattaaacct aactttcaaa agggagcaaa gtacgggatg ttccattcta
     6001 cctttgacgc ttttgaaacc tttctttttg ttcccaatag ggtaaccacc aagggaagtc
     6061 acataaggga ctctattgac atgaagcgta ccatgacggt ggtgatagta gcgctactgc
     6121 ctgccctctt ttttggtatg tggaatgttg gctaccagca cgctctctcc attggagaaa
     6181 gctggccttt ctggcagacc ttcttttttg gctttttacg tgtgctaccc cttttagtgg
     6241 taagctatgg cgtggggctt tcagttgaat ttgcatttgc ccaagctcgt ggacacgagg
     6301 tcaacgaggg atttctggtt tccggtttgc ttattccctt gattttgccc gttgatgttc
     6361 ccctttggat ggttgctatt gctaccgcct ttgcggtgat tgttggcaag gaggtgtttg
     6421 gcggtaccgg gatgaatatt tttaatcctg cactgcttgc cagggcattc ctcttttttg
     6481 cctatccctc gcacatgtcg ggcgataagg tttggattga tgggctagcc aaagggcaac
     6541 aggtagtaga tggtttttcc ggggctacgc ctttggctaa tgccgctgcc taccagctcg
     6601 acaaaattcc ttctgcctat gatatgttta tgggatttat tcccggttcc ataggcgaaa
     6661 cctccaagct ggccattctg atcggggcaa ttctacttat tatcaccgga ataggcagct
     6721 ggaagattat catatcggtg tttggtggcg ggctggtagc aggcctgctg ttcaatacct
     6781 tggccattaa tccttacatg agcataccgc catggcaaca cttcctcatg ggtggttttg
     6841 cgtttggtgc agtttttatg gcaaccgatc cggtaagcgc ttctcaaacc gagcgtggca
     6901 aatggatata cggattcctg gtaggcttta tggccatcct ggtcagggtg ttaaaccctg
     6961 cctatcctga agggatgatg ctttccattc tgcttatgaa tgcctttgcc cctctgattg
     7021 actactacgt aattcagggg aatattcgcc gacgactgaa aagaactaat agggttgcta
     7081 aatcataatt tttttcacta tgagcatttt tggaaaattg aagagtatgg acaagaacag
     7141 caacctgtac accatacttt acgcttccgg gcttgtggtg ctggttgcag tactcctggc
     7201 gttagcagct actcttctga aacctgcaca gcaacggaat gtcgaaaccg aaaagaagat
     7261 cgatattttg aagtcgattg gcaaggccga tggggttgag aaggcagcca acaagcataa
     7321 gtttgtagcc agtaattttg aacgctacat tgttgagcag cttgttgtga actcgctggg
     7381 tgagagaatt cagggagttg atgcctttaa cgttgattta aaagtcgaat tatctaagcc
     7441 attagaaaat agatatttgc ctgtttttgt ggcaaaactt gatagcggtt ccaccaagtt
     7501 tatctttccg cttaggggca aagggctgtg ggggccaatc tggggttaca tctccctgaa
     7561 cgatgatttt aacactattt acggggctac attctcgcat cagggcgaaa cacccgggct
     7621 tggtgccgaa attgcaacgc cacagttcca aaaacagttt gacgggaaat caatttttga
     7681 caaccaaaat cagcttgtct ccattaaggt ggtaaagggt ggtgcagaca agagcgatcc
     7741 ccatggagtt gacgccgttt cgggtggcac aattacaagc aaagggcttg aggcaatgct
     7801 ctacgattgc ctgagcatct atatgccatt tattaacaac caaaaacagc agagtcatga
     7861 gtgatgtgaa ggaaaaagaa ccctggtttt cggctaaaaa tattgagctc ctaacaggtc
     7921 cgttaaattc caagaacccc ataacagtgc tggtgctggg tatatgctcc gctctggcgg
     7981 ttactgttaa gcttgagcct gccattgtta tggccatatc agttacggtg gtaactgcct
     8041 tttcgaacct gattatttct ctaatccgaa acactatccc cagccggatt agaatcattg
     8101 ttcagctggt tgttattgca gcattggtta tactggttga tgaggttctg aaggcttatg
     8161 tttacgacgt gagcaagcag ctctcggtat ttgttggtct tattatcacc aactgcatag
     8221 tcatgggaag gctcgaagcc tttgctctgg gcaacaaacc ctggcctgcc tttctcgatg
     8281 gcattggaaa cgggctgggt tacgggctga tactggtagt tttagcattt ttcagggagc
     8341 tgcttggatc cggaactatt ttcggctaca gggtcattcc tgagtcgttt tactacagca
     8401 atggcgggat gtatgaaaat aatggtctga tgattctgcc cccaatggcg cttattctgg
     8461 tgggcctaat catttggatt caaagaggtc gaaaccgtaa gctaattgag gagtaaaagc
     8521 taaacaatac ggattatgga aaatctgatc aacatatttg ttcgctccat ctttattgat
     8581 aacatgattt ttgcatactt tctgggaatg tgttcctacc tggcagtatc aaaaacggtt
     8641 aaaacatcta ccggattggg catagcggta atatttgtga tgctaatcac cgttcccgtg
     8701 aactacctgc tgaataggtt tgttctactt cccggcgccc tacaatggct cgacgagagt
     8761 ttcgcttcgc ttgaccttag ctttctttcc ttcatcatgt ttattgctgt aattgcttca
     8821 atagttcagc tggtggagat ggtggtggaa aagtactcgc cggcactcta caactccttg
     8881 ggtatttttc tcccgttaat tgcggtaaac tgtgccatta tggggggatc actatttatg
     8941 cagcagcgcg aatatgaaac cctgggtgag gctactgtat tcggattggg ctctggagtc
     9001 ggttggtttt tggcaattat tagcattgct gccattcgcg agaggctcaa gtactccgat
     9061 atacctaagc ccctaagggg tcttggaatt tcatttatcc tgacagggtt gatgggtatt
     9121 gcctttatga cttttatggg tattaagctg taaatggaaa atttttcgag ttatgatact
     9181 acttgccggc aatagttcaa taattttggg tgctgttatt gtatttctca ttgtaacgct
     9241 tttactggta ttgttgctgc tttacgccaa ggcaaagctt actcctaagg gcaaggtgaa
     9301 gctggaaatt aacaacgaga aagtactaga ggtggaacct ggtagctcca tactttcaac
     9361 cctgtccaac aacaaaattt ttctcccctc ggcgtgcgga ggaggcggaa cctgcgggtt
     9421 gtgcaggtgc agagtggtgg agggcggagg ctcaatgctg ccaaccgagg caaacttttt
     9481 caaccgtagg cagcagcggg aacactggcg cctgggttgt caggttaagg ttcgcgaaga
     9541 cctgagcatc atagtgccag aggaagtgct tggaattaaa aagtgggagt gcgaggtgat
     9601 ttctaaccgg aatgtggcca catacatcaa ggagttcgtg gtgaagctac ccgatggtga
     9661 gaaacttaac ttccgctccg gcggctacat tcaaatcgat gttcctaaaa tcgaggtaga
     9721 tttttctaag gatatattcg tggagcccga gtttcacgac gactgggaca agacgaatat
     9781 gtggagcctg aaaatgaaaa ataccgagga aaccttcagg gcatactcca tggctaacca
     9841 tccggcagag aacaacatca ttatgttgaa cgtgcgcatt gccactccac catgggacag
     9901 ggagcggaat acctttttaa aggttaaccc cggcgtttgc tccagctaca tattttcccg
     9961 aaagcctggc gataaggtca ccatttccgg gccatatggt gattttttta ttaaagacac
    10021 acagaacgaa attatgttta ttgggggagg agccggaatg gcccccatgc gttcgcatat
    10081 ctttcatctg ttcaacacgc tcaaaaccaa ccgtaaggtt accttttggt acggagcccg
    10141 ctccaggcgc gaaatattct acgaggagca tttccggaag attgcccagg agttccccaa
    10201 ctttaacttt accattgccc tgtccgagcc caaacccgag gatagctggg atggccctgt
    10261 ggggtttata catcaggtta tatttgagca atacctctcc aggcacaatg cccccgagga
    10321 cattgagtac tacctttgcg gacccccggc catgattgct gccgttcaga aaatgcttta
    10381 caaccttgga gtccccgacg agatgattgc ttttgatgat tttggaggat aaccagcctt
    10441 taaacttatt ggccgaacta tagcatgaga cacatgatat ccggatttca gaaaaaaacc
    10501 tgttgttaaa ccattgtttc ttatataccc tcagtgtttc attgcaaagc agccgttctt
    10561 ttggatgaaa ataacaaaat gcagtagaaa tcctacttac gaacaacttg taattagaat
    10621 gccgaaaacg tcaacggtaa agtcaggcac ctaaactcaa ccattggctt ttcaggaaaa
    10681 gtagtaacgt gatggcaata gttaaactaa taggaattac ggtcggattt ctgatgctgg
    10741 gtatgctagg cttgggaatt agagtaatct ttcatagaag ccacaaattt ccggaaacct
    10801 cagccggtca taacccggag ctaagaaagc gtggaatatc ctgtcctaaa agcgacgaac
    10861 aaacgtgctg gggcggagag aatagctaca caggttgttc tgattgcctc gaacgttcaa
    10921 aacactgaag ctgtgttaga atacctaatg caagtttaac cttaatgaga aacaatttga
    10981 attttttaaa ataaacttat tacgagtagg tgatatcatc atgggaacat taaaattgat
    11041 acttatttca gctgccatgc tggcagttgt gattgtggga ctttcgttga agttgatttt
    11101 cggaaaaaca gccaacaaat cctgcaaaag caacgatgtg ggctacagct gcggatgtgg
    11161 tggaagttgt ggcatcaact ctccgaatat aggcgaacag ctataagttg ttcattagtg
    11221 ttcggattat ccggattcat aaaatacatt ctgttgcatg tgcaacttct ggggtttatg
    11281 gttttattta cttccccgaa gcagccaata caccaacttt tactgccgga taaacggatc
    11341 tgtctatttc ggattattgt ttaatgcgct gatatatagt ttattaatgt gtataattta
    11401 agaattgaag catttttcga cctaaagcta cccattgtaa tcctcgctgt actgtatcgc
    11461 atttcgtctg gtaacatgtt tcaaacctga acaattgctg gcgtaggcaa gttggctata
    11521 aatgattaaa aaacctaatg cctgtaggta atccttctca cttatattct cccggaattt
    11581 ggactgcacc taagcaacgc caaatagtag gctgtagcca atggacattt taacttatcc
    11641 atagtttcct gctaatttga catttacgtt aggtttatgt tgcaataatt ttgcattttg
    11701 gaaaatctat agctaacttt gtagaactta aatgtggaaa tgaacgtcga ttcaagcgaa
    11761 atatatgaca gaattaagca aaaggttcag gttcttgttg ttgcatacga gcgagctaaa
    11821 caggaaaatg aacagcttaa ggctcagaaa gccgaactcg aaaaacagct gaaggctaaa
    11881 gagcaggcat acgttgaact tgaagaacgt tattcccgtt taaagctggc taaggcgctt
    11941 aaggtttctg aagctgatgc gcatgaagcc aaaattaaag tgaacagaat tgtgcgggaa
    12001 attgacaagt gtattgcttt tcttaacaaa taggccagat acagttaaat ggaggatagc
    12061 gagaataaat tctcaattag agttaacata gccgataggt attatccctt aaaaattgat
    12121 tgggatgatg aggagaaaat acgcagggcg gctaagttaa taaatgataa ggtactgcag
    12181 tacaagcaac gatacaccga taaggatatt caggattttt tggctatggc atccttgcag
    12241 tttgttatca ggctaattga gtgtgaagaa aaattctccg gcgctgcttt cgacgaggag
    12301 ctctccaagc tggacgactg gttggccgca tacattgaaa agcaaaacat acgttctttc
    12361 taatagcatt gcccgcatta ctgctctaat attttgcgaa actcaacatt actaaatttg
    12421 gagttggact ttattcgata aggacaggcc tttttaccgc ttagctcggg attcctttag
    12481 tagggacgtt ggaagtccga cccggatggc cgctccaccc atgtaaatct ggggtttcta
    12541 cataaagtta gagtgtaatg cgggtttttt tatattaacc cactaataaa taatatgtta
    12601 tggagttaac aataatagtt gccgtcatcg ccttcatttt aggtggtgct atatcatacc
    12661 tgctatggaa caagctgctg gccagcaaga gaagtaaaat catacaggat gctgaaaccg
    12721 aagctgaagt tattaaaaag gagaaaattc ttcaggccaa ggaaaaattt ctgcagctca
    12781 aggcggagca cgaaaaaatt gtatccgaaa ggaactcaag gattgcgcag gccgaaaata
    12841 aaattaagca gcgcgagaca atggtagccc agaaatatga ggagctgcaa cgtaagcaaa
    12901 aggaggttga tgcaattcgg gacaccctta ccacccaaat ggagctggtt gataagcgaa
    12961 acgaggagct ggagagaatg cacagggaat cagttgaaaa gctggaagct ctttcgggtc
    13021 tctcggctga ggaggccaaa acccagctca ttgactccct taaggctgaa gccaaaactc
    13081 aggccatgtc gtacatcaac gagattgttg acgaggccaa aatgactgct gccaaggaat
    13141 caaaacggat agtggttcaa accattcaac gtgttgcaac cgaagcagca gttgaaaatt
    13201 cggttaccgt gttcaacatt gactccgatg agattaaagg gcgtatcatt ggccgtgagg
    13261 gccgaaacat ccgagctctc gaagcagcca cgggcgttga aattattgtt gacgataccc
    13321 ccgaagccat catcctttcc gccttcgacc cagttaggcg cgagattgcc cggctggcgc
    13381 ttcaccagct cgtaaccgac ggccgcatac atcctgcccg aatcgaggag gtggtgcaaa
    13441 aggttcagaa gcagatagag gatgaaatag ttgaaattgg aaaacgtacc accatagacc
    13501 tgggcattca cgggcttcac ccggagctca tccggttggt tggcaagatg aagtaccgtt
    13561 cgtcgtacgg ccaaaacctg cttcagcact cccgcgaggt ggccaacctt tgcgccatta
    13621 tggcctccga acttggcctt aatgctaagg ctgccaagcg tgcagggttg cttcacgata
    13681 ttggcaaggt tccagacgat gagcccgagt tgccacatgc cattctggga atgaagctgg
    13741 ccgaaaagta caaggaaaaa ccggagattt gtaacgctat tggctctcat cacgacgagg
    13801 tggagatgac aacccttatc gcacccattg ttcaggtttg cgatgccatt tcaggagcgc
    13861 gcccaggcgc ccgaagagag gtagtggagt cttacatcaa gcggcttaag gagatggagg
    13921 atcttgcgct ttcctatccc ggcgtactga aaacttatgc cattcaagcg ggtagagaac
    13981 tacgggtaat tgttggcagc gaaaaggttt ccgatgagga ggccaacaag ctttccttcg
    14041 agattgcccg taaaatccag gatgagatga cctatcccgg tcaggtgaaa attaccgtta
    14101 tccgcgagac cagggctatt agctatgcaa agtagcagcg aaatcttctg gctgatagta
    14161 ttcacacttt gtccattccc atttttttta taaggcagat tcataagtga tggccttatg
    14221 ggatggccat tttgaaatga gttgcagcaa tgattcgaaa gggcaatatt atggtttgac
    14281 tttcggcacg atatgggtaa taagctattc ataaatacat gaaaaatata ccggaatgaa
    14341 caattcatca attcagatgt gcgaccttcg agggcagtac ctgaaaatca aacccgaaat
    14401 tgatgctgcc attcagcagg ttatagatag cacggctttc attaagggta aggaggtagg
    14461 ggatttccag gacgaacttg cccgctatct gggggtaaag catgtaattg gatgcgccaa
    14521 tggcaccgat gcccttcagg ttgcccttat ggcgttgggg cttaaacccg gagatgaggt
    14581 tattacccct gactttacat ttattgccac cgttgaggtg gtagccttgc tggggttaac
    14641 gccaatctta gtggatgttg acccatttac ctttacaatt gatacaaaaa aacttgaaga
    14701 ggcaataacg cctaaaacaa aggccattat tcctgttcac ttgtttgggc agtgcgccaa
    14761 catgggcgaa ataatgcgta ttgcgcaaat tcacaacctc tatgtggtag aggatgcagc
    14821 ccaggcactt ggtgccagct actaccattc tgatgggcat gtttcaaaag ccggaacaat
    14881 tggccatata ggcgctacct cgtttttccc atcaaaaaat cttggatgct atggcgatgg
    14941 cggtgcgctt tttactaacg atgattcgct ggcagcggag attcgttcca ttaccaacca
    15001 tgggatgagg gtacgatacc atcacgacag gattggagtt aactccaggc tcgacaccgt
    15061 tcaggcagcc attctgcggg ttaagctgaa gtacctggac agctataacg aagcgcgaat
    15121 ggccgcagct cagctttacg atacccgcct taaggtatgt gcaaacctgg ttatcccgtc
    15181 aagggctaca tggagcaacc atatctttca ccagtacacc ctaagggtta acgaggctaa
    15241 ccgcgaggag ctgtcaaaat ttcttgccgg caaaggtatt ccctcaatgg tttactaccc
    15301 aattcccctt catagccaaa aggcttttga ggcttacagg ttgcaaaact caaacagcac
    15361 atttccggta tccaactcgc tatcaactag cgttttatcc attccaatgc acaccgagct
    15421 ggatgttgat actattaatt ttattaccga aagcattttg tcattcttca aataagctac
    15481 ttttacattc aaaattttta caggatggaa gtccactatt tcgcgcatga aacagcggtt
    15541 atcgacagtg atgcaaaaat tggacaggga acaaaaatat ggcattttag ccatattatg
    15601 tcgggttcgg taataggtga gaattgcaat attggccaaa acgtggtggt gtctcccggt
    15661 gttaccctgg gccggaatgt taaagtgcag aacaacgtat caatttattc aggggttacc
    15721 tgtgaggatg atgttttttt aggcccctca atggttttta caaatgtgat aaatccccgc
    15781 agcgccatca accgaaaaaa tgagtacatg cccacaattg ttaaacgagc agccaccatt
    15841 ggtgctaatg ccactattgt ttgtggcgtt accttgggtg aattttgctt tgtaggggca
    15901 ggagcggttg taaccaaaga tgtgaaaccc tatgcgctgg tggtgggaaa tcctgccagg
    15961 cagattggct ggatgagcga gtttggccac aggctggaat tcaatagcga taaccttgct
    16021 acctgccctg agagcgggca ggtttaccgg ttggataata atcaagttaa caaaattagt
    16081 taaaaaatga aaaactttgc attgattggc gctgcaggct ttgtggcgcc acgccatttg
    16141 aaggccataa aggaaactgg aaataccctg ctggcagccc tcgacaagca cgactgtgtg
    16201 ggcataatgg acagctactt tcctcaagcc gatttttttg tggagtatga gcgcttcgat
    16261 cgccacattg ataagcttaa acggcagggc ataaaagttg actacgtgag catttgcacc
    16321 cctaactacc tccacgactc ccacataagg tttgccctgc gacagggagc cgatgcaata
    16381 tgcgagaaac ccgtggttct gaacccatgg aatattgatg cccttagtga aattgagcat
    16441 gaaaccggca ggcatatcta cactattctt cagcttaggc ttcatccggc cattattgag
    16501 tttaaaaaga aaattgacaa tggccccaag gataaaattt acgatgttga cttaacctac
    16561 atcaccagcc gtgggcgttg gtatttcatt tcgtggaagg gcgacgttca aaaatctggt
    16621 ggagttgcca ccaacattgg agtgcacttt tttgatatgc ttacttggat ttttggggat
    16681 gtcaaaagtg gcgttgttca tatgtctatg cccgataagg ctgccggtta ccttgagtta
    16741 caaaaagctc gcgtccgttg gtttctaagc attgattaca acgatattcc cgacacagtg
    16801 aagcagcagg ggaaacgaac ctaccggtcc attaccgtag ggaatgatga gcttgagttt
    16861 agcgagggtt ttaccgattt gcacaccacc agctaccgcg atattctttc tggaaacgga
    16921 tttggcctga atgatgcccg tagctcaatt gaaacggttt atgcgattcg caacgctgtt
    16981 ccggtcgggc tcaagggcga ttatcatcct ttttgcaaga agtaatagaa tataacaaca
    17041 ttcactatat gttcaaggag ttagtttcaa agaatgaaaa aatggcggtt ataggactag
    17101 gttatgtcgg ccttccgatc gccctggaat tcgccaaaaa aatttcggta gttggattcg
    17161 atattaaacc cgacagggtt gagctgatga agcgtggagt tgacccaagc catgagcttg
    17221 aatctacgtc gtttcagaac tgcgacataa catttacttc tgatattaac gatttacgaa
    17281 aggcgtcatt ttacgttgtg gccgttccca cacccattga tgagcataac cttcccgatt
    17341 tgaaaccatt acttgccgct acccgaacgg taggtcaggt gctgaaaaaa ggtgactacg
    17401 tggtttacga gtcaacagtt tatccgggtt gcaccgagga agattgtgtc cccattcttg
    17461 aggagctttc cgggttaaaa tttattcagg actttaaggt tggattttcg cccgagcgaa
    17521 taaacccggg cgataagaat cacaccctta caaccataaa aaaaattacc tcgggctgcg
    17581 atcctgaatc agccgaggaa atagctaaaa cttatgagtt gattattaaa gcgggcattc
    17641 accgtgccag ctccattaag gtggccgaag ctgcaaaaat tattgagaac acccaacgcg
    17701 atattaacat tgcgtttatg aatgagctct ccatcatatt cagccgcatg ggtataaaca
    17761 cctacgaggt gctcgaagcg gcaggaacca agtggaactt tcttaacttc tcacccgggc
    17821 ttgttggagg ccattgcatt ggagttgatc catactacct ggtttacaag gccaaggagc
    17881 tgggctatca tccgcaaatt atcagcgctg gtcgttacat caacgatacc atggggggat
    17941 atgtggccaa gcaaaccgtt aagaaaatta ttgctgccgg aacaaatcct atggatgcaa
    18001 gggtgctggt tatgggtgtt accttcaagg aaaatgtaag cgatattcgg aactccaagg
    18061 tagtcgacat ctaccgtgaa ttaagatcgt ttggagttaa ctatatcgat attattgatc
    18121 cctatgccga tgcccatgac gtaaagcacg agtatggttt tgacatggca actgctccat
    18181 cgggcaggta tgacgcaatc attctagccg ttgggcatag ccagtatgct ggactcaagg
    18241 aggaatggtt taagcgcctt gctgccgaaa agtgcgtttt tgtagatgta aagggtattt
    18301 ttaggagcaa aattaacaac ctcacctact ggagccttta ggtggtttga tggatagttt
    18361 ggctggtatt tctactaatc cgtactaaac atggcataat aatctggcca tgtttgcctt
    18421 aacgggctaa tgccataaac cagccccatt cgtgaatgcc ttgcaaattt ggagttaata
    18481 gtattacttt gcaatcttta acttttatta caagtatatc aagtttataa tgatggtaaa
    18541 gattactttt cctgatggta gcatacggga gtatcccctt ggggtaaccg ggattgaaat
    18601 tgcccgaagc atttcggaac gactggctgc cgaggttctc tcaatcactg ttaatggtga
    18661 ggtttgggat ttgactcgtg gaattactac cgatgccacc gtaaagctgc acaagtggga
    18721 tgacgaagag ggaaagcata ccttttggca cacatccgct caccttatgg cgcaggcact
    18781 cgaggccctt tacccgggca ttaagttggg aattggccct agcattgaca acggttttta
    18841 ctacgatata gattttgata gctacactat ttctgatgac gatttcccaa aaattgagca
    18901 gaaaatggcc gagctggccc atcaaaaact tgatgttgtt aggaaggatg tttcaaaaga
    18961 cgaggcacta gccacataca aggctaaagg cgacccatac aaagttgagc ttatcagcga
    19021 cctacaggac ggcaccatta ccttctacac ccagggcgat ttcaccgacc tttgccgtgg
    19081 gccacacctt ccaaacacag gctacattaa ggctattaaa attttaagta ttgcaggagc
    19141 atactggcgt gggaacgaga aaaacaaaat gctaacccgt atctatggca taacctttcc
    19201 acagaaaaaa ttgctcgacg agtacctggt aatgctggaa gaggccaaaa agcgcgacca
    19261 tcgtaagctg gggaaagaaa tggagctatt taccttttcg cagcatgttg gtcaggggtt
    19321 acccctatgg ctgccccgtg gcgcccagct tcgctttcaa ctcgagatgt tccttcgaaa
    19381 agtacagcag aagtacggct acgaacaggt aataactcct cacatagggc aaaaggaact
    19441 ttacgagatt tctgggcact gggcaaaata cggcaaggat agctttagac ccattactac
    19501 ccctcaggag ggggaggagt ttctccttaa acccatgaat tgcccacatc actgtgaaat
    19561 ttataaatct aaaccccgtt cctataagga cttaccggtt aggttagccg aatttgggac
    19621 ggtttaccgc tacgagcaaa gcggcgagct tcacgggtta acacgcgtta gggggtttac
    19681 tcaggacgat gcccatatct tctgtcgccc cgaccagctc aagcaggagt ttgtcaaggt
    19741 gattgatatt gttttgtata tttttaaaac cctcaatttt aaggagtaca ccgcacaggt
    19801 ttcactccgt gacccaaagg atagggaaaa atacattgga acggacgaga actgggaaaa
    19861 agccgaaagc gccattattg aggctgctgc cgaacgggga cttaaaacta ccaccgttgt
    19921 tggcgaggcg gcattctacg gacctaagct tgatttcatg gttaacgacg ccattggccg
    19981 aaaatggcag ctggggacta ttcaggttga ctacaacctg cctgagcgct ttcagctgga
    20041 gtacattggc tccgacgaca agcgctaccg cccggtgatg atacatcgtg ccccatttgg
    20101 ctcaatggaa cgctttgtgg ctgtgcttct tgagcatacc ggagggaggc tccctatttg
    20161 gcttgcaccc gaccaggttg tagtgctgcc catcagcgaa aagtacagcg attacgcaaa
    20221 aaaagttttt aattatctaa ataattccga tattcgcacc ctgattgacg accgtaacga
    20281 gaaaattggt aagaagataa gggacaacga gttacgccat attccctacc tgcttattgt
    20341 gggcgaaaag gaggaggaaa acggcacggt tgccgttcgg gttcaaggtg aaggcgataa
    20401 aggacaaatg tccctcgatg attttattat gctggttagg aataatattg aaagtcagct
    20461 tactgaaata tagttaacta taaccaatag gaggacaaag ctatagcaac accattacat
    20521 tcccaggtta aaaaggacgt agataataaa ttcctggtaa acaatgaaat aagaaacaag
    20581 gtagtgcggc ttgtcgggga aaatatcgag aacccgggta ttttttccat cgaagaggct
    20641 aggcggctgg ctaacgagct ggagttagac cttgtgatga tttctgataa ggccgatcca
    20701 ccggtgtgcc gcattattga ttaccagaag tttctctacc agcaaaaaaa gaaacagaag
    20761 gaaatcaagg ctaatgcaca gaaaatcgtt gttaaggaaa ttcgttttgg cccaaatacc
    20821 gatgagcatg actacaactt taagctgaaa catgcccaga atttccttaa agaaggggca
    20881 aaagtgaaag cctacgtatt ttttaaggga cgttcaatac tttttaagga gcagggcgaa
    20941 atattactcc tacgatttgc ccaggattta gaagaccttg gaaaggttga gcagctaccc
    21001 aggttggaag gcaagcgaat gatcatgtta atatctccaa aaggagcaaa gaagtaaata
    21061 aacctaaata atttcagttt agaatgccga aaattaagac tcattccggt gccaagaaaa
    21121 ggttttcttt gaccggaaca ggaaaaatta aaaggaaaca tgcttacaag agccatatcc
    21181 ttaccaagaa ggaaaccaaa caaaaaagaa accttaccca ctttaccctt gttgacaagg
    21241 cagatgagaa aagggtcaaa aaaatgcttg ggctcaggta gccatgactt tagcctttta
    21301 tttacagcta cttttaatta ctaaccagaa tgaattagcg cctaagtgtc cgggttgcgg
    21361 aacgctaacc attcaaaaat tttaaaacta tgccacgttc agtcaatgct gttgcatcaa
    21421 gaaagagaag gaaaaaaatt ctgaagcaga ccaagggtaa ttttcttgcc cgtcgcaacg
    21481 tttacaccgt tgctaagaac acccttgaga aaggtttaac ctatgcctat cgggatcgaa
    21541 agaagaagaa aattgagttt cgcgctttgt ggattcagcg tattaacgct gctgttaggc
    21601 cttatgggtt aacctactcg gaatttatgg gtaaaatcaa caagaagggt attgcgatta
    21661 accgtaaggt tctggccgac ctggccatga acaaccccga agctttcaag tcaattgttg
    21721 attctgttaa gtaggtttcg ccagcatatt cagcataacg ttttggaggg gtaagcaccc
    21781 ctcctttatt ttaacccatt ccctcgacga ttgcctgtag caatgataac aaatattata
    21841 gctatattgc agaaaaatta ggctttatgt tgaggctggc tataatcgga tacgggaaga
    21901 tggggcatga ggttgaatcc atggcgatgg agagaggcca tgaggttact ttaagaattg
    21961 atagggataa tactcatgag ctaactcccg aaaacctgaa attggtggat gttgctgttg
    22021 agtttacctc gcccaagtcg gcaaaagcaa atgtggaggt atgtctgaat gccggagttc
    22081 ctgtggtttg tggaacaaca ggttggggta atgagttgga ggcggttata gagcgggtta
    22141 aagtgggtgg gggaacgctt ttttacgcct caaactatag tgttggcgtt aatgttatgt
    22201 ttaagattag tgccttgctg gcccgatacc tcgataaaat tggcggttac tcggtgggca
    22261 ttaaggaaat acatcacctc caaaaactgg atgctccaag cggcacagct attaccattg
    22321 ccggtaaaat tactgaagaa atcccctcaa ttaccggttg gacgttactt cccaagcgtg
    22381 aggatggcaa aatacctatt gaagccataa gggaagggac ggttccagga actcacacca
    22441 tttcttttag ctccgatcag gatagcattt tattaaccca cgaagctaaa agtaggcgtg
    22501 gttttgcgct gggagccgtg atggctgcag aattcctgca caacaaagtg ggttttttta
    22561 ccatggacga tctgcttgcc tttgattaag agggtaacgg tatgcaatct ttttctttta
    22621 tatcaacaaa taattatagc ttaaagcggc atgcttaaaa taactaatgt taatatgagg
    22681 atattgtaac ccctttaggc tacaaccagt tcaattaaat aatagttttg cagcgtaaac
    22741 ttcagcttaa ggtaatatga ccacgaaaat tcgaaccttg ttcaaaaaca gatacttcaa
    22801 cctagggttg tcgtccctca tctacatttt aatggttatc tggataggta actattggct
    22861 actactagga ttagccgtaa tttttgattt ttacatcacc cgtaaggtta actggacatt
    22921 ttggaaaaaa cgggggcaaa agaggacagt tcttattgaa tgggttgatg ctattgtttt
    22981 tgcggttatc gcagccctgc ttatccgcat gttcctgatc gaagcctata ctattccaac
    23041 ctcatcaatg gaaaagaccc tgatggttgg cgattatctt tttgtcagca aggttagcta
    23101 cggtcctaaa atgcccaata ctccaatttc attccccttt gttcacaaca ccctgccgtt
    23161 ttcaaagaag gccaaatcgt atgtggagtg gcctcgatgg ccatataagc ggctggccgg
    23221 gctgggtagt ataaagcgta acgacgtggt ggtattcaac tttccagagg gggatactgt
    23281 tgtgctgcag gatcccaact ctagctacta caccctggcc aggttttacg gccgcgatta
    23341 cctttggcaa aactttgata tcgtttaccg gccagttgac aaacgcgaca actacataaa
    23401 acggtgcgtt gccattccgg gcgataccgt tagggttgtc catggccagc tttacgtgaa
    23461 tggaaaagag caggtaaaaa tcggcgaaag gcaatacaac taccgtatta taacgaatgg
    23521 gggaacgatt aatcctaaac gttttgaaga gttgggcatt gcaaaagccg atcaaaacta
    23581 caaccctgct gaatcggagt atataatccc attaactgaa ggtatgctcg atcgaattaa
    23641 ggactacggc aatattgtaa agattgagag gatggagaat accgattcaa ccaggatgtg
    23701 gcaaatgatt tttcctcata acccaagcta tccatggaca gaggataact tcggcccgtt
    23761 gtggataccg tccaaaggag ctactgtcgg cctaaacttg gataacctgc ctctgtatga
    23821 gcggataatt gatgtgtacg agaataacga cctggctgtt cgtaatggag ctatctatat
    23881 caacggaaag ccttccaatt cctacacttt taaaatggac tactacttta tgatgggcga
    23941 caaccgccac aactcggccg atagccgatt ctggggtttt gttcccgaag atcatgtggt
    24001 gggtaagggt tctttcatct ggttatcact cgataaggat aggcatttcc ccttcaacat
    24061 ccggtggaaa aggatttttt ccggcattca ctaaccttca atcgttataa tgtgtttgcc
    24121 atcagcatca ttttacctcg cttaatcatc taatggtgga atatttactg gttgatgtag
    24181 agggcataat aataactcaa ggaaatagtt ggaccgtaaa gtagttttat caacagccta
    24241 cctgccgccc attcagtact ttgccaagct cctggcatat gatgttgtat atattgaatc
    24301 agcagaaaat tacgccaaac agtcgtaccg taaccgttgc accattctga gcgctaacgg
    24361 ccccttaaac ttatcggtgc ctgccgttaa gtcgctggga aaccatacac caatcactaa
    24421 ggttggaatt gacaactcgt ctgaatggca gaaaaatcac tggcgggcag ttgaatcggc
    24481 ctaccgttct tcaccctttt ttgattttgg tgccgattta ctactccctt tctacaccca
    24541 aaggttcgaa aatctttttg agctgaacat ggagctgctt aaagttctac tcgggttcat
    24601 aggtgtggtt gttgatgttc ggtttactac aacctatttg acatcctatc cggaggaata
    24661 ccatgattgc cggtatttta tccacccaaa gcgttcattc aaagcagacg accacatggt
    24721 ttgggttgag tactaccagg ttttctcgga caggtttggc tttgtgccca atcttagcgt
    24781 tattgatttg atttttaacg agggtttagg cgcaacttcc attctgaagt ccatgaataa
    24841 gtaaacctgc ccgggtgttg ggcaggttgt acgggttaga tggtgatatt tgtaatctta
    24901 gaatctgaat cccagggtta tcaggaattt tccatccctg gttttggtgg aaaccacatt
    24961 gagatcggtc aaatcgtaaa atttatactt agaattctgg agagtttgca ggtatgctgc
    25021 atcaatggta aagttaccta tccggtaacc aaggccgaat gaaagctggt tggtgttgga
    25081 attatcgttt aaaaaacccg acttgtaggg cgatgggtag taagcataac cggcccttac
    25141 ggaaaaatcg ttaattcgtg cctcgccgcc tacacgtatg ttggtaacat ttctgaaatg
    25201 accgtcgatg ctctggttta aatcagtaaa gttatcgcca ttccccccat cccgaaatct
    25261 catggctgag tagtctaaat gttccacatc caggcttaca agcccgtatt cttttataat
    25321 ataggcaaag cttccaacga gtttgaatgg ggtttcaaag tcgtagtcat atttacccag
    25381 aggcgaattg ctggtaaagt tactctcaac tccggcaatg tcgaagttgg atttcatggt
    25441 atatgagtag ctgtcgctga acgagaagaa agtgggagtg tggatggctg caccaattct
    25501 tatacttggt ataggggtgt atatagcccc gagtttaata ttgtagcctg ttccgctagt
    25561 ttcaaagtac tgcatgtagt cggagtagta gaagcgatcg ccgttggatc gtgcagggtt
    25621 ggattgaaag gcatcctcac tgtaggtcgc attgtaggtg aagctgtaat cggcaatacc
    25681 tactgttaat ccaaggtaga gcttatttga aatgtttgca gcaaatgaaa tgtcgaactc
    25741 accggaaccg cccttagtgg atacagcgtt tttctgaact actccgtcac caataagtaa
    25801 tgctgggtcg tactctgtac cattcgtacc cgctatcgtg tcgtatagca agtatgtgtt
    25861 ccatgccata atcacatccc agggagcact gctttcgcta aatggattgt aattatcatc
    25921 aaccagcaaa tcgttatagt agtacccctt agccttaaca gcaaagaagt ccataatgga
    25981 gttgttggga ttatcgccgg tggcaatgga gttggagaaa aagtcgtttg tgcgggtgta
    26041 cccaactccc atgtttaggt taaccaagcc cttatcgtcg cttccgttcg gtttaagcga
    26101 cattacaaca cccacgttat cgaagtacag gcgattcctt gtgtccgtag ctttaatgcc
    26161 gttataggtt gaagaaacct tttggttttt aaatgaaggg gtaacggtta gctctcctga
    26221 cttgtaaaca cccaatcctg ccggattgat gcttagggag gtgaaatcgc cccccaaggc
    26281 accaatggct cctcccagcg aaatgctgcg ggctgttcct ccattaaggg tttgcgaaaa
    26341 tcgaattcca tcctctatgg tttgtgaaaa aagttgagtg acagtacctg aagccattaa
    26401 taccagaaaa aatctcgaaa tatgccttat cattgtaata ggtttatgta gatgctaatg
    26461 attgtttttt caccacagtg tataaattgc tggataaagg gaggaatttt gttattcctc
    26521 cctgctacac agctaagaga tttttatcgg ccacgtgttt tggctccaga ggatcccgag
    26581 ttgctatttc ctcttgaacc tgatgacgag ctttcagttc ttggagtgta agttccgctg
    26641 gaatttcccg aagacctgga accctcaggt tttgactgca ccgaatttcc tgatcgtgtt
    26701 gctgagccat ttgaaggtgc tgaaaccctg ccggggctat tgtatgtggg agaggaagac
    26761 gatctacccg atggtgatgt ggttgtacct ctgccagtgc ttggacgctg gatagactgt
    26821 gttccggatg agctggttcg gggtttatcg tagccagggt tgtatgctct cccggttggc
    26881 tcggatattc caatggagcg cgtaggcttt tcgctccgcg ttccagaact tacagctgaa
    26941 cctcttgtag atgaagggtt tgatagagta gtgccggttt ctctgcgggg agcagtaatc
    27001 tgttcgcccg aacgttgagt accttctaca cttcctctgt tgtttttata ggtgggtgtt
    27061 acgtgtgttt gggtgttttt ggtagattga ataggattta tggagctgct ttgcacgtca
    27121 cctctctggc gggtaacagc ctggctgcta gttggcgtaa ctcctgaagc tgttcggcgg
    27181 gaagaagtgt tgaaggatga ttcacccggc cggcgaccat agtagaagtt tccgctgtta
    27241 aggtagctgt attcaatacc gtggtttatc cagtagtttc ctggaaaatt ccatgctgag
    27301 tagtagtaag ggttgtagta gttgaatggg ctatagaaga aggggtcgta gtaccacgac
    27361 catggattat agccccatcc atacccgaag ccgaaataga atgaggttct gggccacgag
    27421 aaggagttgt agatgtacca gggttcaacc caaacctgac tacccattac cactagcctg
    27481 tagtagttgg ggtcataggc ctgagcgtag cgatagctat ccgagtagta caccgaccag
    27541 tcgttcagcc cgtacatagg gtcttccatt ccctttaacc tacgttcata cgactcctga
    27601 taggaatcgg agagaatgct ttcgtatggg ttgacgtttt ccttgtcgga tgaaatggtg
    27661 tcgttttggg tggtttcctg gtttacggcc agctcgtcgg catacttttt gtccagctgg
    27721 gcgtaatcat ttaaattagt ttgaacagga gtgctatcct tactatcctg ggcaagttct
    27781 gttttggaag ttttggtgtt tgccctgtaa atctcatcct cccacgatga ggtattgcta
    27841 acctgcaggc tcgacgagca ggacagcgta gtaaatccca gtagggcaag cattggaata
    27901 tagttttttg ttttcatggc gcacctcctg tttgaaaatt atgttattat tatggttact
    27961 tttgctagct gaaaaatcat ctaaatatca acaaaaatca taccaataga acgatatggc
    28021 aaaggaaatt actagtagaa acgagaatta ttctcaatgg tataacgata tagtgatcaa
    28081 ggctgggttg gccgactact cggcagttag gggttgtatg attattaaac cttacggcta
    28141 cgctgtttgg gaaaaaattc aggcacaact cgacaggatg tttaaggaaa ccggccatca
    28201 gaatgcctac ttcccacttt tcatccccaa gtcgtttttt agccgtgagg catcgcacgt
    28261 tgagggtttt gccaaggagt gtgctgtggt tacacattac cgcttaaaaa atgatgcaag
    28321 cggtatgggc atagtggttg acgaggatgc caagctggag gaggagctga tagttaggcc
    28381 aacatccgaa accattattt ggaacaccta caagtcgtgg attcaatcgt acagagatct
    28441 gccaatcttg ataaaccagt gggctaacgt ggtgcggtgg gagatgcgta caaggctatt
    28501 ccttcgtacc accgaatttc tttggcagga ggggcataca gctcatgcca cccgacagga
    28561 ggcagtagaa gaagcggaaa ggatgattaa cgtgtatgcc caatttgcac gtgagtggat
    28621 ggccatgccg gttatcatgg gtcggaaatc ggaagccgag cggtttgccg gagccgttga
    28681 cacctactca atagaggctt taatgcagga tggaaaagcc ttacaagccg gtacatctca
    28741 cttcctgggg cagaattttg ccaaagcttt cgatgttaag ttccttaaca gcgagggagc
    28801 gcttgattac gtttgggcta cctcgtgggg agtttccacc cggcttattg gtgcgctaat
    28861 catggcccac tccgacgata acgggcttgt acttcctcca aaactggctc ctactcaggt
    28921 tgtctttgtc ccaatctata aaagcaggga ggaacttgat agaattagca gtgttgtgtc
    28981 gcagcttaga aatgaacttc atgcagtggg ggttactacc cactacgatg atagggataa
    29041 ctataagcct ggattcaagt ttgccgaata tgaaatgaag ggtttcccca ttagaattgc
    29101 cattggccca aaggatttgg aaaatggaac tgtagaggtt gcccgcaggg atacacttca
    29161 aaaacaaatc gttagcagca attcattagg aaagtttatt atagggttgc ttgatgatat
    29221 ccagaataac ctgtttagta aagcgcttga atttcaaagg caaaacacta ccgttgtcaa
    29281 cagctatgat gagtttaaga gtgtaattgc aggtaagggg ggatttgttc tggctcactg
    29341 ggatggaact cccgaaacag aagccattat taaggacgaa acgaaggcaa ccatacgctg
    29401 catccccttc gacagcaagc ccgaggaggg aaagtgtatt gtaaccggta aaccctccat
    29461 gcaaagggct ctttttgccc aggcatacta gggttgaatt atttaaaatg cttttgtata
    29521 tttgattgtt tttttaatac atggggtatg gccgaagaac agcaaccaac caaggagcaa
    29581 atattcgatt tactaaacgg taagtcggtt ttaaagcaaa aaatctatga caacaccttt
    29641 gaggtgttgg gtaaaattaa gcttgtgctt cgcgatttgg ccaatgagta caacgccaac
    29701 cttaaaaata cggacaggcg ggttaggctc gagtaccgcg atagaggtaa gtttgaggca
    29761 gaaatccggg tggcaggcga tcttctcatt ttcagcatgc acaccaacgt ctttgagttc
    29821 gatagagacc acgccatctg gaagctttcc tacgtgaagg atgacaagtt tgcctcctac
    29881 tgtggcgtta tcaatatcta caacttttta gttgactctt ttagatacaa cagaattgac
    29941 gatttgggct accttgttgg ccgaattttt atcaacaggg attttcacta ctttgtagag
    30001 gggaaacggc aaatgggttt cctttacaat aactttggaa ctgccgtagt tgacatgaaa
    30061 tcgcttcgac aaatcgttga aaccgccatt atgtactcgc tggaattcga tttgttggta
    30121 ccaccctacg acctggttaa aattgcatcg gttcagcaga tgaacagcaa gattgaaagt
    30181 tcgaacatgc aaaccggcaa acgtttggga ttcaagttta actccgacga tgtgttggag
    30241 gaagaaaaca agtaggctat aaaccagcta atttacaacc accatgaaca gattgtgtat
    30301 tacgtttttt gccgcactga tggctctctc atcctgtggt acaaaacaca acaaggaggt
    30361 taaatccgag atgcagaaaa aattggacga atttgcgcct gttgagctga ggtcggacat
    30421 cactcatttg tcaactcgcg aaattcaaat gctgccctac ctatttgatg ccgctcagat
    30481 aatggacgat ttattctggt tgcaggcatt tggaagtaag gaagagtttc tcgacacaat
    30541 taccgataag gccacctttg aatttgccct ggttaactat gggccgtggg agcggttgaa
    30601 cgacaataag ccctttattc aaggttttgg cgaaaaaccc ctaggcgccc aattttatcc
    30661 tcccgacatg accattgagg agttcgaagc ctttagctct cccgacaaaa ccagccagta
    30721 caccctcatc aggcgcaact ctgatgggaa gcttgaatcg gttccctacc atattactta
    30781 taaggatagg atagacaagg cggctgagct aatggtgcag gcagccaact tagccgagga
    30841 tgcaggcttg aaacgatatt taaatcttag agctaaagcg ctgcaaaccg acgagtactt
    30901 tgaaagcgat atggcctgga tgaacatgaa aaataacaac atagattttg ttgttgggcc
    30961 aattgaaaac tacgaggacg cactgttcaa ctacaaggcc gcctacgaag cctacatcct
    31021 gattaaggat cttgaatggt cacaaaaaat tagccattac gccgctctgc ttcccaagct
    31081 gcaggagagc ctgccggttg atcctgcata taagaaggaa gttcctgggc ttgattccga
    31141 tttgggcgtg tacgatgttg tgttctatgc aggcgactgc aatgcaggaa gcaaaacaat
    31201 tgccattaac ctaccaaacg atccaaaagt tcagctcaag aagggtagcc gaaagctgca
    31261 gctgaaaaat gccatgaggt acaagttcga taagattctc ctgcccattg gcaacttgct
    31321 gatagacgag gagcaacgcg agcatatcaa atttgatgct ttttttgaga acgtaatgtt
    31381 tcatgaggtt gcccacggct tgggaatcaa aaaccgggtt gatgggaaag gtggtgtgca
    31441 ggaatcgctt aaggagatgc acagcgcact ggaagagggg aaggccgata tacttggtct
    31501 ttacatggtg tcgcagctgg ctgccatggg agaactcccg gataaggaca tgatggacaa
    31561 ctatgtaacc tttgttgccg gtattttccg atctgtacgt tttggagctg cctcttcgca
    31621 tggaaaagcg aacatggttt gcttttacta cctcaaggag cgtggtgcct ttgagcgaaa
    31681 tcccgaaacc ggaacctacc gggttaactt cgataaaatg accgaagcaa tgaactccct
    31741 tgcccacgat atcctaatac ttcaggggaa cggggattat gagggtgtta aaaaactttt
    31801 tgccgataag ggatttatag ccgaggagct acagcatgac ctggatcggg tttcggaggc
    31861 tagcattcca cgagacatta gatgtattca aggcaaagaa atatttggct tatagcttgt
    31921 aaaggtcggg ttctccgacc ttttttttga gttcatattg aaatatttta attttaacgg
    31981 ctacatttat ccaaattttg gagacctaaa gctatgaacc taaccttcaa ctttatcaag
    32041 tcggtgtcga acatctacga tgagatgatt gataacgggt tttcactggt gtatctgggg
    32101 gaatttagcc atgaaataac caagatgttt acctccatgg ccgaatcgga tatggagaag
    32161 aacagcgagg aaagatcggt tcagcgtaag gtgtaccatg ttatggttga aacccttcag
    32221 aatatgaaca agcattccga tgaaatcaaa gagcgaaaca ttggcaacgg gctcttcatc
    32281 atcgggaaaa aacatgacac ctactacgtg attacctcca ataaggtagc tcgcaagcat
    32341 aaagaccatc ttgagcaatc gctgatcact gttaataacg cttcgcccga agagttaaaa
    32401 gaaatgtata aacggcaaat tcgcgaagga acgttatccg aaaagggtgg tgccgggtta
    32461 ggactaattg atattgcccg caaaacaggc gaaccattga actaccaatt ccttcaactc
    32521 gatgaggata actatttttt tatacttaaa gtcgaaatta atgcccgtaa gttctacaag
    32581 taagtggtta aaggagtaat cctatgtctt attaatgggt attttgtcgc ttaaaaaaag
    32641 ctttttacag ctaaccttac gcttaggatt agcaataacg gttcatttat catttctgct
    32701 tttcacctcc acttcttttg cattaacaac cagcaagttt gattctgttc agctattttg
    32761 gaatcccgac acccttagta gaagcgacac caacttgttc aatcgtcgag ggaatgaggt
    32821 tcttaagtcc aataatgcca tagtaaataa cctgttagac ctaatttact gtgaggaaga
    32881 ggatactagg caaaaagaaa tttatgccga acaggcaaag gcatatcaga agttctcaag
    32941 atatgctgga agaataatag ccgatattca aattgtacgg ttaaagcctt ttggggagta
    33001 cattaacgat actacactaa aacccgaaaa cagccttcag aaatttggga actcgctaca
    33061 ctcgcttaca ttacgccggt cggttgaaaa caacctgtta ttttccgagg ggcaagaact
    33121 tgacccggtt gacttagtgg aaaccgaaca gctccttcgc aaccttctct atattgaaga
    33181 tgtaggtatt aacattgaac ccctgcagga cactaactat gtgaaagcta cggttgttac
    33241 gaaggataag tggactctga acatgggggt acggattgaa agttcagcga agggacggat
    33301 aaccctttcg gagagaaact ttgccggaat aggtataggg gcaaccgggc gtgtttacta
    33361 cgacgaggaa caaaaggaga aatgggggta catgggtgag ctgaatatcc ctaacattgc
    33421 tgggactttc attaccagcg acttgtggtt ccgtgaaggc gtcggctaca aatcttactg
    33481 tgtagatgtg aagcgtgact tttacgcctc aaaaaccaag tatgcactag gcggaaccta
    33541 tgttaaaagt caagaaccct acaaaatttt tcctgttgat tcaatagttt cgatagacta
    33601 tagcctaatg agcttttggg taggccgttc atttagggtt ggtaaaccgg ttataattgc
    33661 ttcgccttac atttttaccc ttgctctaaa gtaccggcag gttgatttct acaaatccct
    33721 accaacatct atgaacctaa acccgtactt tcactctaac taccaagttt tgttagcagc
    33781 cggattttct aaccaaaatt ttgctcagga taacttcatc tactcttttg gcgcaaccga
    33841 ggatattccg gttggattta tagtgcagct tattggaggg gttgaggata gcgagtacca
    33901 gcgacgattg gtggcaggtg gtgaaatttc ggctgccgag ataacacctg ccggatacct
    33961 ttttattgct gctcgtaccg gaggctacat ggccgaaggc ccacgagccg agcaaggaat
    34021 gatcaacttc aaatctactt ttatttctaa taaatttacc cttggaaaat attttggacg
    34081 gcaacatgtt ggtgttagct ttacaagggg cttttcgcgg tttgaggggg aaagggaata
    34141 ccttgcactt aacaaggata acgggattac cggtctaaga agcaattccc ttgtgggtca
    34201 aacacgccta atagttaatt ttgaaacaac tttgtttagc ccacatttat actatggttt
    34261 tagaccatcg tgttttgcct tctgtgattt gggaatggtg ggaggttcaa acgaagtaat
    34321 ttttggcaat tctcttttta gcggtttcgg tctgggtttt aaggtgaaaa atgaaagttt
    34381 aatctttccg tcatttattt ttcgtcttgg ttactaccct aatgtccccc aaaatgccga
    34441 tgttgcctat tggattatta cctctcagga tcgtaagaag tttgagcagt tcaggatgaa
    34501 agagcctgcc ctacttccct tcgagtagcc tggcatctgc gaacaatttt catttcacgt
    34561 tgtttaccat gaaaatttac aacccggcat aattgaacat ttctggttaa ttgcatggtt
    34621 gtattaagtg gcagctaaag tcatttttga ttttacatta ttaaaatacc taaaaattat
    34681 tgaactatgg gagtactagt aggacagaaa gcgcctgttt tcaaggctag cgcagttata
    34741 aagggtcacg aaattgttga gaatttttct cttgagcagt tcatcggcaa gaagtatgtg
    34801 gtattctttt tctaccctgc cgattttact tttgtttgcc caacagaact tttagcattt
    34861 caggataagc tggaggagtt tgaaaaaaga aacacacagg tggttgcatg ctcggttgat
    34921 tcggagtttt cgcactggaa atggctgcaa accgaacaaa aagatggcgg cattaagggg
    34981 gttacctacc caattgtttc ggatttatcc aaaacaatct ccgagaattt tgatgtgctg
    35041 gccggtcatt acgactacga tgacaataac ggagatgctg tatttgttgg cgttccacag
    35101 gcattcaggg gattgtttct gattgacaag tccggggttg tccgccatca ggtggttaac
    35161 gatatgcctc tgggaaggaa tgtggacgag gtaatccgta tggttgacgc acttcagtat
    35221 ttcgaggagc atggcgaggt ttgccctgcc aactggaaaa aaggtgaaaa ggccatgaaa
    35281 gcaacccagg acggcgtttc ggactaccta agccataggt agtaagccta cttgcatatc
    35341 cacttaactt gctgaaccgc ctctaggggc ggtttttttg tgctaaatcc ttcatcttta
    35401 atgctaattt tgtggcatga ggaaacggtt aacatacctt gtcgaaaacc ctgaagtggt
    35461 taccgacaac atggctaaag ccctgctaag ctgggatgtt ggcattgttt taagaggaaa
    35521 ggatgctatg ccggttccgg gaacagacat ggtaagccag ttcgattgtg cagcaggatt
    35581 tggtgtggct gccagctgca aacctgcgga taattttttt aaaaacttcg aatactttac
    35641 cgatgctaat aacgattgga ttttaggttt tttaggttac gaccttaaaa atgagctgga
    35701 gaatcttcaa tcggggaatt tcgatgggct ggcatttccc gaaatgttct tcttcgttcc
    35761 ggaagtattt atgagtattg ctgaccacaa tctttgcgtg tactactatc ccggtaaaac
    35821 ctctgaccta actgttagca agcttgttga cgacctgcta agagttgaat tggttgctaa
    35881 ccctcaacag cctaccatca cttttaagtc cagatactcg agatatgaat accttaccca
    35941 ggttcagcgc attaagaacc atatcggcag gggtgatatt tacgagatga atttttgcca
    36001 ggagttttac accgaagggg tatcaattca gcctgcccgg gtgtttaacc gcttatatag
    36061 caactcacct acaccgtttg gcgcattttt aaaaattggc gaaaagtttt taatggcagc
    36121 ttctcctgaa cgttacctta ggaaaaacgg gaatgtagtg gtaagccagc caattaaagg
    36181 cactgcgcca agaatggctt tgccagacat ggattcgcag gtggtggaaa agctaaaatc
    36241 ggacgccaag gagatatccg agaatgtgat gatagttgat ttggtccgaa atgacctgtc
    36301 gagggttgcg gttcccggtt cagttaaggt taacgagctc tgcggaatct acaccttccg
    36361 gcaggttcac cagatgattt ccaccgtttc gtgcatcgtg cctgagaagc taaacctggt
    36421 agaacccata aaagccacat tccctatggg atctatgacc ggtgccccaa aggttagggc
    36481 aatgcagctg attgaagagt ttgaaaaatc caggagggga attttttcag gcgcagtggg
    36541 ctacatcact cctaacaaaa attttgattt caacgtggtt atccgtagct tgctttactg
    36601 cgggcaaagg aagtaccttt ccttttcggt gggaggtgca attaccgcca agtccgaccc
    36661 tgaaaatgaa tacaacgaat gcttgctaaa agccaaagcc atacttcaaa cgttaaacgc
    36721 caggattgaa aatgttgcag gataaattta tcaatttcat tcgggtacac cggttggccg
    36781 acgagaacga ctccatcctg ctgggtgtta gcggtggcat tgattccatg gtaatgctgc
    36841 atctttttca aaagactggt ttcaaatttg ccgtggccca ctgcaatttt gggttaaggg
    36901 gtgaggagtc tgatggcgac gaggagttta cccgaaccta ctgccgtgaa aaaggagtgg
    36961 tgttccacac tactaaattt agtacggttc aatacgctac tgataagagt gtttccattc
    37021 aggtggctgc cagggagttg aggtatgact acttcaacga gctttgtcgg gagcacggct
    37081 acaacaaaat tgccattgcc cataacatga acgacagcgc agaaacggtt ttgctcaacc
    37141 taatccgagg ggcaggtatt agagggttaa caggtatcag gcctgttcat ggaaatataa
    37201 ttcgaccgtt gctctttgcc actcgccctg agattgaaaa ctactcaaag cagctgggaa
    37261 ttagctaccg tgaggattcc agcaacgcaa ccctaaagta caaacgaaat ttcataaggc
    37321 atagggtcat tccgctgcta caagagttaa acccttcagc ggtggaaaca atttacgatt
    37381 cgtcttatca tgtcaatgag gctatatcgc tggttgacag gcagctggac gagataagaa
    37441 ggaaagttgt ttcattcaga aaggatgctg tgctattttc agttgaagaa ctaaagcgtg
    37501 aaccggcagc ccgtatattt ctggttgagg agctacggca ttacgggttt tcgggaaata
    37561 tggcaacaca agtctttgac ctgctggagt caactccagg aaagtatgtt gactcgccga
    37621 ctcataccct gctaagggat agaaaatacc tgattctgtc ggctaaaatt tctacaagta
    37681 atgcctgcat agaggttggc gaagattgca gccggattga gaaacccttt cccatgaggt
    37741 ttgagcgtat tgttgaggtt aagattgatt caagtccgca agtagccatc cttaactact
    37801 ctaagctaag gtttccgctt acacttcgat gctggcaacc tggcgatagg tttatacctt
    37861 taggcatgaa aggctataaa aaggtaagcg attaccttgt tgactgtaag gttcctttaa
    37921 accaaaaaaa cgaggtgtat gttctgcttt ccaataatga aattgcctgg gtggttggct
    37981 acaggattga cgacaggtat aaaatatcca ctgaaacagc tgaggccttg aaaatttcgg
    38041 tgcttttgta gcaatacaac ggcagaaatg caaacacaac cacccccctt taaggcagcg
    38101 gtacctgatt acgtgaagtt taaccttttg aagagtggga gactagtatt cttttcggga
    38161 agtttctaag cctttatcgt tgtaaattat ccatgttccg gatttctccc ccatccggta
    38221 ggtcatatcg tagcgtaaag tccccttctc gtcccacaca taccatttac catttttctt
    38281 tccgttgaga aagttggctt cagcaacttt taccccctga gcattccatg tttcccatct
    38341 tccgcttttt tcccctttta tgtacgaccg aatttcattc agctcacccg acggaaaata
    38401 gacttttacc tgcccgttga gtaagccctg gccgatttcc atttgagatt ttacctgtcc
    38461 gttttcaaaa tactcctgat atgtgccagt gaagggttta ccattgctgt caaggtatag
    38521 cccattcttt tctgtaacat tattttgggc accggacaga aaaggaacta aaagtaaaag
    38581 taacgtaagt cttttcatat tatggtgttt agcaggttga tagttannnn nnnnnnnnnn
    38641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    38701 nnnnnnnnnn nnnnnnnnnn nnnnnntaag tatcggtgtt aaagctgatg gaacatccat
    38761 attttggctt caccaatccc gtaagaagcg tacggttgtt catgtatgct tgtgcatgaa
    38821 gctctacgag ggtatttcat aagaaatcat ttttatttat tatttacagg caaaaagcaa
    38881 aagggttaaa aaggccaggc atgtactggc taaaacaagt tttacctatt tgtaattttg
    38941 actaaaaaat caaatgcagt ataaacgatt agaaaggtag tagtatattc aaaaaaaact
    39001 tctcccggat taagggagaa gcttttaagg ttggttataa cattagtatc tagcgttctg
    39061 tggatcccag ctgcaccacc cgctggtcca gtttgtagtt ccaaaaccac caatgaaggc
    39121 aactgtttcg aagccagaca acccgctaaa ggaagcgcct gtgagaacag gagaaccact
    39181 ttggggcata ggattaacat ttccccagct ccatgcattg gtaagtttta tatctgcatt
    39241 ttcagcaaag gtctgattgt tacctgctgt ccacatggcg tcatactgct cctgggtaag
    39301 ggtagtccca tcggtgttaa cctgaaaatc ctttatcatg ccggccatta tgcagttctt
    39361 aatttcagga ccccctgtag tgttgtaaag cgccatgcta gggccttcaa tacgagggcc
    39421 agttgcaaaa cctgccagga tagagttata aaggttaaga cgtgtattac gacgaagacg
    39481 aagggcatgc ttgtggttgg ggttaacatt tggttgggtc ttagtggcat aggggccaag
    39541 aatagtgatg ttgctgaact ttgcagaggt taatggagta tttgatgagc cgtttgcatc
    39601 gttatcggat tcaaacccgt tggatgcatc ggacttgtca gcttcctctg gaccacgaag
    39661 tattaaacca tactgaacat ttccgctaaa gccattgtcg gtgtcaaaat cgtcatcacc
    39721 accacggtac gaaaccaggt acttggcatt tacggttccg ccaaaccact cgaaagagtc
    39781 atcgcggcca aaagaaacct gcacgtgttc aattacagtt ccacttccta cgccccccaa
    39841 ggtaaggccg ttaatttcat ttccgctgga aacctcgtat cctgcaaact ctatgcgaac
    39901 atactgaagc gatccggagt tgtcggcagg atcagtaccc ccgtaaatag cgccggttcc
    39961 tccttcaatc tcagcctcac caccggtaat attaatagga gccttgccac aaattattat
    40021 tcctgcccag tcgccaggtt ttctctgacc ggccggttta tcgctagtaa atacaacagg
    40081 tctatcttgt gttccattgg ccataatttt accgccacgc tcaactatca gcgctgcctt
    40141 ggtgtcgctt aaacccttaa ttagcgctcc cggctcaatg gtaagcgtaa ccccattgtt
    40201 cacgtaaaca aacccttcca gcatgatagt atcgtttgcc gaccaggttg tgttggctgt
    40261 gatatcgcca gaaaccgtta ccttttgtcc tgtgggaggc ttgggttcgt cttcatcatc
    40321 attacatgat gccatgccca gaccaagtag gattgccgca aaggctaaac cctttgtgct
    40381 tgttaagaga ctcatttttt tcattttgat tgattttaga attatctggc acaaaagtag
    40441 ggctgagcag ctaaagcgca gctaagcaaa aattaccctt tcattagctg tttgttaggt
    40501 atctgttaac agggaagtgg tagtgtttct agaatgaaaa agccacacct gggtgaaggt
    40561 gtggcttagt ggtgaactta gctacgatga gttactctac tcagcaatgg gttccagctc
    40621 aatctttatc ttcagtgtag ggttgatagt tttatttatg tcaaccttta caactgaagt
    40681 agaataggag ataagttcgg cactaaggtt ttcgagtttc tccggattaa cttgtatgct
    40741 gaagttccca tcaaaatcgg agtagcaagt tgtgcttcca tcagttttga cggcaacccc
    40801 tgccagtgcc tcccctgtat gcttgtcggt aactttcccg ttaatggtga ttgacttaac
    40861 gggcaagctg gttgtgcttt caccttttgt tttatcatca gtggttgctc caaaaacatt
    40921 caaggttaaa gcagctatta acagcactag aattcttttc atgggtattt gttttacttt
    40981 tgtttctgaa gcaaaggtag tatggtggta tttccttacc attatatcaa tatgattaaa
    41041 atgttaagtt gatgttaaac aggttttggt gctaaaaatt cagtgaaaag cccaggctaa
    41101 atgttatccc gggctgcatt tccctactgc ttaaatttaa atcacctcca tcagcctgtt
    41161 taaacgcttg gtacttgatg ctcttttgat tcaagagatt tttgatacct gcttttattt
    41221 ccattttgct gccaattccc ttgctggcgg tgaagtcaac caggtggcta tgtttttcgg
    41281 tgatatcggg gatgtcctgg ttggagtttt ggaatggtag ccccacagct ataatcctat
    41341 cacctataag attatactgc aacgatagat ttagcttatg cttggtgttg ctgtaaaaca
    41401 ctgctgcatt aaccaggtag ggcgattgtc cctgcatggg tctatcacga aatatagagt
    41461 ttgcaggaaa gtttacccgg ctgtatatgt atgaggcatt aagcacagcg ctgaaatcct
    41521 ttagcagggg aataaactcc agtgattttc gtagctcaac ttctgctcct gcgctttggg
    41581 cattatctgc attatcgtag gtaaatgtta agcggtttcc ggcgggtctg aacaccgatt
    41641 caattgagga tgtaaagtgc ttatagaaga ttcctataga aataatttca ccggcattcg
    41701 ggtatagctc gtagcggaaa tcgaagttcc acacatatgc gttttttaga tttacatttc
    41761 ccacgtaatc ggcttcctcg tcaaagttgt agaagtaaaa tggtgcaatt tccctaaatt
    41821 ctgggcgatt aacggatagc ccgccggcta gacgcagcaa atttttctca ttgaagttgt
    41881 aggtaaggtt cgccgatggg aaaatatcta tgtggtcgag tttaacgtca acgggcttgt
    41941 tgtacatgtc gtagctgttg aggcgcatgt tgttcatttc gaatctcgct cctccgtaaa
    42001 atctcagctt ttgggttata ttcgtgttga tggtcaggta tcctgcaagg agcaggttgg
    42061 tggcgtcgta tgagtctgat ctggaagtca tctcctttag tataaaaccg cttggggtag
    42121 tgttgatgtt ttccaggcta aaaattgttt caaccggaag ccagaccgat tcggtgtagg
    42181 agacattttt ggcgaaacct agtattctgg cgtcaaatgt tctattttta tattcagagt
    42241 aaagtccggt tttaataaca ggtttccagc tcccaatttt tattttagct tcgtagtttg
    42301 tccctgcagt ggctatgtat tcgtgaaggt tcatgaatag cctgccggcg tcgctcactg
    42361 aaggtgttaa cccaactgag gcatagtatt cgttgtagtg gggaagtagc ggctcagact
    42421 gtagggtggt tcgaagttgt tttaagtcgg gttcgttacg tcccgagtag gcaaatccta
    42481 aaacccagtc aagggtatta tctgagttgc tgcccagctt atgctctcca cccagctgtc
    42541 cggagtaggt tatcctgctc atgtatttat cctggtatga ccggatggta taaccttcat
    42601 agccatttag cccattccga ataacggcag tattgtttcc catcaggttg aagagatttc
    42661 taaactcgat ttttgttcct tttccaggga acagcgacca gttatgtaga atccccagcc
    42721 tgaccgagcg ggagtatgtg ctgtcggtat agttatagct gtatggagga atttcgcctg
    42781 ggttaagctg cacctggtaa cctttgttta ccagctcatc gctactgtag gtatggctgt
    42841 agcttatgga tgttatgttg cccagcaaac ggttaccctt ttgaattttt ctgccaaagc
    42901 tgaagttgag cttttgctca ggaaaagcgc ttacaccttt tggtgcccag ctggtgttta
    42961 gcctttttcc caaatcggcc agctcagcat cagggtaacc ttttagggaa tttggaaatc
    43021 ctgagggtag gtttcgggtt ccgccatcca cccctaacca atctgttttc ccggtggtta
    43081 tgagctgaaa atcgccatag tgtgcccggg tattgtaacc cgttgtgtag cttatgctca
    43141 tgaagttgct ttcgggcata tttttggtaa aaattttaat gaatccaccg gtaaaatcgg
    43201 ccggtagctc aggcgacatg ctcttggata ccatcatgtt atcaatcatg gcgctgggaa
    43261 taacatcaaa cgagaaggct ttttgatcgg tctcgctcga aggcgttgat gcgttgttta
    43321 tccagacgct gttgtatcgc tggttcagcc ccctaactac aacaaatcta tcatcaatta
    43381 ttgaagctcc cgggacacga cggataacct cggaggcatc cttgtcctgt gaattgttta
    43441 tttgctgcgc cgatatgcct gttgccacca ttaggctcgc ctttatagag ctaatcatgg
    43501 ctaggttggt gctggttttt cgggtggctg ttaccgtaac accctccaac tcagtagtga
    43561 tttcctcaag ctctatgcta acggtggtgg ttttattggc ttcaaccctt atcccatcaa
    43621 gtattgttgg cttataggat ataaatgatg cctggacgct gtaaattccc ggctccaaat
    43681 tttccagctt aaactttccg tcaaagtcgg taatggtacc cttaaaagta ccctcgatga
    43741 taacgctggc ccctaccagg gtttcgtctt ttcccatatc gacaaccgtt cccacaatag
    43801 atccggtctg ggccgatgct gatgttgcaa gtaagaaaca tagaagagtg aacaaatagg
    43861 ctattcgtct gttattagtt ctcatagttt ataaacggtg gtttatattt tttgctgcaa
    43921 agaaactggt aaacttcaac ctgaagatta acgcaacttt aatatattgt taaccgccat
    43981 caggaatgag gccggatttt ttaatgttaa attaacttta ccattttatt ttataaatat
    44041 ctttgtatca ccataaaatg cttaaaacgg gataaatgag taaacctggt taccgaattc
    44101 ttattgtaga tgatgagccc gacattattg agtttttaag ctacaacctg accaaggagg
    44161 gctatgaggt tgatactgct acaaatggaa ggcgggctat agagaaagca acagaattca
    44221 gtcccgatct tattttgctt gatgtcatga tgcccgatat ggatggaatt gaaacttgcc
    44281 aggagttacg ggcaaatccg ttaacaaaag actgcctcat tgctttttta actgcacgta
    44341 gcgaggacta tagccagata gcaggctttg acgcaggggg cgacgactac atttcaaagc
    44401 caattaagcc caaagtgcta attagccgta ttaaggccct gcttaaacga tctggctcgc
    44461 tggaggaagc acctgaacca acacccgaaa ttcctgagga gggaatctac atcaacaggg
    44521 aacggtatct tgtttacctc gacggcaggg agctggttct tccaaaaaag gagttcgagc
    44581 tgctggccct gcttcaaagt aaaccccaaa aggtttttac ccgtgatgaa atttaccagg
    44641 caatttgggg cgacgacatt gttgtgggtg acagaactat cgacgtgcat attcgcaagc
    44701 tgcgcgagaa gcttggagac caatacatta gaacggtaaa gggtgttgga tacaagttca
    44761 ccgtctaggc agcatctcct caggtagcta taagggaaat ttcttggaag caacctaaat
    44821 taattttacc tatatggagc ttttttcgtg gaaaaacgac tacagcgtta aggttggaag
    44881 cattgactcc gatcatcaga agctgttcga cattattaac tcactgttta ctgccatgtc
    44941 gaagggtgag ggcaggcagg ttatctccgg tttggttaaa gagctgcaga gctacaccat
    45001 ttaccatttc aaccgggagg agacattttt cagggttacc agctacccca atgctgccaa
    45061 ccaccttgag cagcataaaa tttttgttag caaggtgaaa gagtataagg aaaaaattga
    45121 caagggaaac aatgactttt cgcccgaaat gttgacctac ctaaaggatt ggttgcttaa
    45181 ccatatcctt gttaccgaca aggcctactc tgatcacttc acaaagaacg gcattgtata
    45241 gaatccactc tccaacaagg gagggggtat tctttaagct acaatttttc ttaccacagc
    45301 taattttagg ctgataatgg gaaggattat acactttgaa cagggtatca atatttcaga
    45361 ttcttctaat ttcaaccagg agtggtaatt ttatattagt agcattacgg ttataatttg
    45421 atattttcta cctttctttc aactattttc aaaaagaata tttgccatgg agctaatttc
    45481 gtgggaaagc aactacagcg ttaaggttgg cagcatagat gccgatcata agaaacttgt
    45541 ggaaatactc aactcgcttt ttatggccat gtcgaagggt gaggggagta agattatctc
    45601 cgacttagtt aaggaacttc agcgttatac aatttaccat tttagcaggg aagagtcttt
    45661 ttttaggatt accggctacc cgaataccca aaaacatatt gaggagcata agcagtttat
    45721 tggtaaggtg aaagagtata aggaaaaaat tgacaaggga aacaataact tctcgtccga
    45781 aatgttggcc tacctaaagg attggttgct taaccatatc cttgttaccg acaaggccta
    45841 ctcggagcat tttgtaaaac acggaatagc ctgaattgct gatatgaaga agtttggagg
    45901 acgaaatgac ttccaaatag gcttggatag tgtttgcctt tagggcaaat atcacttcta
    45961 tcaatatctc gtcctacttg aagttggcca ccatttccat tcttcgttgg gttcccttct
    46021 caagtggaac gagggttaag ctggtgggta taaccgaata cttgctttcc ggtctaagct
    46081 ggccaagcca cttaaatcct tttagggtca tatcttcggg ttccacttgt tcaataggaa
    46141 tgaggtttga agttggcttc tgcttgaata ccacccggct gaccttattg ttcttaatgt
    46201 ggacgtcaac attagaacct tcagccctgt tcaccatggt gatttgaccc ttgtccctaa
    46261 cgaagtaaac cacctgtccg tttcccgtaa tatccattcg ggtaagctgg ccatcggaaa
    46321 acctggctaa aatggtttta cccctcatct ggctaaagta tgcggagtcc tcacgggatg
    46381 atatgaaggc attgcccaaa aagtcagcct ggcttatctt tttgttgctt atatggatac
    46441 ggatgaaatc ggcagtcatc tggtttgcct cgttccacat aaccgggttg ccatacatct
    46501 ccaaaacaga gtcgcgggtg ttgtagatca tggaatcgca aacaccctga atatcgtggc
    46561 ggtagtacat caccctgcct attgccttta ccaatctata ggatgagtcg gctttctgat
    46621 tatcacctgt tttggctgta atcatgagca gcttgtcggt gcgaaggaat aggctgtcgg
    46681 aatcatcaat catcatcatg tagggttgct cggtaatttc ggcttcctct ttttcctgcc
    46741 agtagctggc cttttggccg taaatgtagg tgcggtttac cgtgtcaacc atcacaacat
    46801 ttcctattcc acgggcatac ccattttgct tatcgtagta aatattttcg ccaaagagtt
    46861 tttgagagtt gcttatgaga taggcgttat tttggaagtt cgattgttgt agttttcggt
    46921 tatgccagcc tttttcgcag tatataaagt tttctccgtc atagataaag gttggtccaa
    46981 aaaagtaggc aacctcattt ttggtgtggt acgaaaggga gtcagtaaag gcattaccat
    47041 cgggactttt taggactact ttattggcaa aggatatcag gttgtcgttg ttgttgtagt
    47101 agccttttat gctggttaag gtggatgagt cgttggaggt tgccactcct ccggtggtgt
    47161 agaaagccag gcttgttttc gagttgaaat ccagggcgtc ggtaaccagt gtaaaatctt
    47221 tgtcaaccat tttaacctcc ttgccggtta gccttccctc ggaggttctc ccgtcatagt
    47281 gcagcttgtc gccataaata ttcgaacttc cctgcgaaac taccacgcca ttaaaggcat
    47341 cgaaccagtt ttcattttgg ctgtagtatg cgctatcgca cctaacacgg gtgttatcgt
    47401 agaaaaaacg gacgttgcca atgtacctga caattttctg atctcccagg ttcaggtttt
    47461 tcatggcttt ggcgccctcc agcttaatgg tggttttctg ctgggcagct gaaaatgttg
    47521 ggtgcaggga cgtaaaccct aaaataaata ggaaaattct ggttgtgctg gttgaagctt
    47581 tttgcattac cacttcttgc tacttaatcc atcctttaga gcgaaagctg cagtttctaa
    47641 atggctcatc aaccctgatg taggttgatt gaattttctt gtccaaccac tcttgcagaa
    47701 tttcgttttc catactaacc ttagctatat tctgaatgat ggcgtagtct tccttaaggt
    47761 tggctttgtg gggtggaatt attcgcttca ccttgataat tttgaatacc acgttggcat
    47821 gttcgtctcg tgattcgaat gggctgctca tttccccctc cttgagctcc ctaatggcgt
    47881 agaagtcggc aggttgaagc tgctcctttt caaaaagaga agtattgttg taggagttga
    47941 tgaccagccc tccgtttaga aacgattttt tatcctgaga aaatctcatg gctgcagctt
    48001 caaaggtcaa gctatcggct ttaatgaggg tggcaatgct atcgagcttt gacatggctt
    48061 gtgccaacat atcacctgag aattggggtt tcatcagaat atgcctaaca tttacctgat
    48121 tgtcgcgttt ttcaattagc tggatgatgt ggaagccata ctcggtttca actatttggc
    48181 ttatttgacc ctcctttagg ctaaacgctg catcggcaaa ggccttcacc agctcctcgc
    48241 ggtaccgaaa gcccagctct ccgcccctga ttgccgatgc cctatcctca gagtaggcaa
    48301 cggccagggt tgaaaagcgc tcgccctgga gtatgcggtt tctgatttcc agtagttttt
    48361 ccctcacctg tagctttgcc tcctcggtag agggtgggta aatcataatt tgttgtagtt
    48421 catactgctc gggaactgtg ggtaggctgt cggttggtat gtttttgtaa aaacttttca
    48481 cctcagcggg tgttatcttc accttatcaa caattttttg ccgcatttgc tgggtaagca
    48541 gctgctcgcc aatagcttca cgcaaatcgt tcttaacttc gtaaatgggt ttgttgaagt
    48601 agctttcaag cgccttctcc gatcccagct ggctggtaaa gtacttcaac cttcggtcaa
    48661 tctccatttc taccgacgac tcatttgtct ttaagctgtc aattttggcc tgggttaaca
    48721 gcaatttctg gataagaatt tgctccagca cgttgcagcg gttgttttcg ccggttggtg
    48781 aaccctccat tttctggcga agaagttcct gctcaatatc ggaaagcagg atatgctcac
    48841 cacctactac tgctacaacc tgatctatta cttgcgcttc ctgcgagtag gcgttaacta
    48901 tgaaaagttg aagtaacaaa gccgttattg ctaatcgtgc tggtttcatt tgtagtattg
    48961 tttaatttct aatgagtagc ttgacattta ccttttataa acttgaaagt tttcatcatc
    49021 ctgcccttta ctgtatatcc gctgctcaag ttcggagata agtttctgct ttcgggcgtt
    49081 cagcacgatt tgcttcaaat cgtctgtgat gtaatcgaaa ggggctgttg agccggaagg
    49141 tatggcatcc ataaacctta ccaggtaaac gaagtcgcca tcctccattt caacatatct
    49201 tttctcaaaa acctcccttt gcggatttgc tattggaaaa ggaagttctc gaaggatgaa
    49261 tgagagggga atccacttat cattaaagta gtcgtacttt gttgcagcct gataggctat
    49321 gttatcgagc tgcttaatat cttcgtcact gtttgacagg tagagctccc ttattttctt
    49381 tatttgtgga gcctccctgc gcagcttgat gtacaaagcc ttgacaattg tctcgtttag
    49441 cacaaaatta tccttgttgt catcgtagta agccttcaga tcggccattt taattacggt
    49501 atccagcttc tggttgaggt aggcctgttc gtacttgaaa attagaagtg aagtacggta
    49561 gtcctccagc tggcgggtaa cattcttttg agaatcggat agattgtttt cggcaatttt
    49621 cagcataagc tgggtttgaa tccatttatc aatgtaggca tctacaatca gggcgctgtc
    49681 ttccttcgat acgttgccta caattaagtt ggcaatgtcc gaagggtaaa ggtagctgtt
    49741 gtaaaccctt gccaggggca tgggcttttt acctgagttg aagcacgatg ccaaaacaac
    49801 tgtcaaccca accagcgaaa aagttactaa acgaaaactt ttcatgtttt atctcattta
    49861 ttgttgtttt gccatgcaga aatctcgtcg aacacactcc tattaactat aacggtgtag
    49921 tgttttttca acttttcgag ccattccctt tcaagcgagt tttggtaatc ggatatcacc
    49981 tctcctttgc agtcggtcag aggttttacg tcaccaattg ttgtgtccaa cacctcaaca
    50041 acaaccacct cgccgttttg tgtatcccta acactgaact ttttaggcca ggaattgtag
    50101 tccgcgacct caggcaaggt gggatcacct gatttttcga ctacggtggc cacttgttta
    50161 gccggcgagt tgaaccgatt cgcataatcc agaggcgtaa gacccttttt ggaacgtttt
    50221 tctataccgc tgacaaacgc ctttttaatt ttcggatcgg taagagtata aacagcgaag
    50281 tgaatgcgtt tgccccactt gtagctatcc tggtgttcgt tgtaaaagtt tgctattccc
    50341 aggctatcag aacttttctg ccacacctcc cggttggaaa tttcgaacag cagcataccc
    50401 tcgtagtact cgcgggtaag ctgggcaaat tcggggtact tatcaggtag cttagacttc
    50461 tcgtagctgg taagagttag gtttacccat tcggtgtagg cattcatgag aaaggcagga
    50521 atagaatgct tgcctattaa cccttggttt tgttcaatga aattgcaaaa gtcagaaagt
    50581 tttagctcgt ttccattgaa gctaataagg gtagagtttg atttgaggtt gggcgatttc
    50641 catgttccct tgtagagtga tgaatccagt aacggaatca gtagcttcaa ctttgaagta
    50701 tcctgcttaa aggaatactc ctttttgaga ttctctataa atgctgttcg gggaagcatt
    50761 cctcgtgaat cgcgcattag catagctctt atttcaggaa taagttcttc cctgttcccc
    50821 agctcctttt ttgcaagtct tttcacaatg tgccaaccag ctgaactttg gaagggttgc
    50881 gatatttccc catcatgctg cagggagaag gcaacagctt caaactctgg aattacctga
    50941 ccgggacgta tccatggcaa ctctcctccc gattttgagg tgctggggtc ttgcgattcg
    51001 tctttggcta gctgggcaaa atcggcaccg gcagtaaggc gttggtaaat acccagaatt
    51061 tttaacctgg catcctcggc tgcttgaggg gaaggattgg gtggaactcg tatcatgata
    51121 tgggcaacct ttacctgtcc gcgagcaggt cgcttgtcgt gcaccttgat gatgtggtat
    51181 ccaaagcggg ttctcacggg catcgagatg gaatcaacag gggtggagta ggcagctgat
    51241 tcgaaagggt aaaccatttg aaatgcggtg aagtagccta gaaagccact gttcctcgaa
    51301 accgagggat cgttgctcat tgcaactgct actgagccaa atgtttctcc ctttagaatt
    51361 cttgaacgag cctccatggc cttctggaag cactttaacg tgtcggaggg tgaagcattt
    51421 tcgggaattg atatgagtat gtggctggcg ctaacctcat actgcatccg gttgtatgct
    51481 tcctcaataa ggttgtttaa tacctgattg tcaattaggt atgactgtgc cagctgttct
    51541 acatatccct ggtactcatt tttaaaagct gcagttgtat ctatcccgtt gtctttggct
    51601 gctttaactt ttaacttata gttaatgaag aggttaaggt aatcttctag aacttcgcgg
    51661 gttggttttg cgttttctat ggatgtactt ttaaggtaaa gtccctgaaa ttctcctgca
    51721 gtaacaggtt cgttgtttat agtaagtaaa attttttgtt cggttggttg ggcaacagca
    51781 gctataccgc aggttattac catgaaaccc gccagagtat tcctaataat tttattcata
    51841 cgaatacttg tttaaagttt actgccaaca aattgcagca gtttcgtttt catcttagct
    51901 attttacacc ttcgccatat tccttaaatt gatggtacag ccaacaacct ttgcgtagtt
    51961 atttttgatt tttaatgctt ctgctggggc atggctgcgg gcaaaataat aaaacgtgca
    52021 agctactagt agtccgtatc gcggctgtag ttaggagcct cacgagtaat ggcaacatcg
    52081 tggggatggc tctcaattat accggcgtgg gtaatcttga caaaccgggc tttttttagc
    52141 atctcaatgc tggttgtacc acagtagccc attccagccc ttaatccacc caccagctgg
    52201 tagaatactt cagagagcgt tcccttgtag ggaactcggg caactatacc ctcgggaacc
    52261 agttttttta tgtcgtcctc aacatcctga aagtacctgt cctttgaacc ctgctgcata
    52321 gcgtcaattg agcccatgcc acggtacgac ttgaatttac gcccgttgta aataatggtt
    52381 tcgcctggag attcctcaac gccggcaaag agcgagccgg tcattatgga atcggcccca
    52441 gctgctaatg ccttaacaat atcgcctgag tagcggatac caccatcggc aatcaccgga
    52501 ataccgcttc cctcaagggc gtgcgagacg tcccaaatcg cattaagctg tggaacgccc
    52561 acgccggcaa taatacgggt tgtgcaaatg gaacctggtc caatacctac cttaaccgca
    52621 tcggctcctg caccggctag catcttggct gcctcggccg ttgcaatatt tccaacaaca
    52681 acgtcaatgt ttggaaattc tcgctttacc agcttgagca tatccaccac acctttcgag
    52741 tgtccgtggg ctgtatcaat cactatggcg tccacatttg cttcgcagag tgcccttacg
    52801 cgttccagcg tatctctggt tacaccaacc ccagcagcca cccttaacct tcccatttta
    52861 tccttgcagg catttgggtt gtccttaacc ttggttatgt ccttgtaggt aagcagtcct
    52921 acaagcttgt tgttctcatc aacaacgggc aacttctcta ttttgtactt tttgagtatc
    52981 tcagttgcat tttcgaggtc tattgatttt gaggtggtta tgaggttgtc ggaggtcatc
    53041 acctgccgga ttggagtgct tacatcttcg tgaaagcgaa gatctctgtt ggttacaata
    53101 cctattaaaa atcccaggct gtcaataacc gggattcctc caatatggtt ttcgcgcatg
    53161 agcgttagtg cgtctccaat agtagcgtct gggctaatag ttatgggttc gaaaatcatg
    53221 ccattttctg cgcgcttaac ctcacgcacc tgtctggcct gtgcttcgat gctcatattt
    53281 ttatggataa cacctattcc cccttccctg gcaatagcaa tggcaagggt tgactccgta
    53341 actgtatcca ttgcagccga aacaatagga gccatgaggt caatattcct ggaaaacttt
    53401 gttgaaaggt tcacattccg tggaagcacc tcggagtagg ccggtactaa aagcacatca
    53461 tcgaatgtaa gcccctcagc tattactttt tccgattgaa acgacatatt catagtttgg
    53521 ttaggttgta aaaatgcggc aagttacaaa aaaatgacaa atagccaatt accatcaacc
    53581 tgcatgcagc ccatggtgaa cttttaaaat ggcaacgccg ttgctcgaag taagcccgtt
    53641 agcccaatta atatttaagc taaatgacct taatttttga tttgcaggtt atagaccggt
    53701 tatttgctaa atttgctcca gcagatttta caactttgga taggtttgag gaaatactaa
    53761 agcagtactg ggggtataac attttccgtt cgattcagcg ggacattatt gaatcggttt
    53821 acaatggaaa tgatacacta gcgcttatgc ccaccggagg tggtaaatct attacttttc
    53881 aagttcctgc tcttgccctg gagggaacct gcctggttat tactccgctt attgccttaa
    53941 tgaaggatca ggttgaaaat ctgaagaaaa gggatattag ggcggttgcc gtgcattctg
    54001 gacttacccg gcatgaggtt gatgtagctc ttgataactg tgcatatgga gatgtgaagt
    54061 ttctttacct ttcaccggag agaacaacca cggagctatt ccgcgaaagg ctcagaaaaa
    54121 taaaggtgag catggtggcc gtagatgagt cgcactgcat ttcgcagtgg ggctacgatt
    54181 ttcgtccttc atacctgcgc attgccgagt tgagggagat tatttctgag cctgttcctt
    54241 ttcttgctct cactgcaacg gctacacccg aggtggttga ggatattcag caaaagctga
    54301 agtttcgcaa tggcaaggta ttcagcatga gttttgccag ggaaaatctt gtttacctgg
    54361 ttaggaatgt ggaggataag ccaaagcacc tgctcaaaat aacgcatggc attccgggaa
    54421 gtggcattgt ttacgtccgt agccggagta aaacccgaga aattgcccag atgcttcaaa
    54481 aagaaggcat ttcggccgat ttttatcatg cgggcttaac catggaggtg cgtaatgcca
    54541 agcaaaacga ctggcaagtt ggcaaaacca gggttattgt tgccaccaat gcttttggca
    54601 tgggtattga taagcccgac gtccggtttg tagtccatgt tgatcttccc gattcgcccg
    54661 aagcatactt ccaggaagcc gggcgtgccg gccgagatca aaagttggcc tatgcggttt
    54721 tacttttcaa caatgccgat tcactgaaaa ttgagcagcg tgttgagtca aactttcctc
    54781 ctattgagga gattaagcga acctaccagg cattaggttc gtacctgaac gttgccattg
    54841 gtgccggcaa gggcgaaacc tacgatttca accttatgga cttttgctca gcctataagc
    54901 ttagctccgt tagggcttat agctcgctaa aaattcttga gcgtgaaggg taccttgaac
    54961 ttaccgacga gcttgataac cccagccgca tcatgtttct tgccggtaag caggatctct
    55021 acaagttcca ggttgctcat gcagaactgg atgggtttat taaggcgata ctccgaaatt
    55081 ttaccggagt tttctcccaa tacgtccgaa ttgacgaggt ttaccttgcc cggctggcgg
    55141 gtattactgt tgataaagtg atcgaaaacc ttaaaaccct tggcaggtat aaaatcataa
    55201 gctacattcc caagaaaaga actccgctgc tggtactcgt tgaggagagg cttgacgata
    55261 aaaatttgcg cattgaacca agctcataca ggttgcttag gtcacgcttt gaggataggg
    55321 taaaggctat gctttcttat gcaaataacc aatccaagtg cagaagccaa atgcttagcg
    55381 aatactttgg ccagaaggac tcctaccgct gtgggaaatg cgatgtatgc acatcgcgca
    55441 acgagttgga catgagcaag tatgagtttg atatgattct ggatgggctt aaaaggcgct
    55501 tgcaagctgg gccatgtagc gaggaggagc ttatagatgc aattaaacgt aatcctgata
    55561 gagtagccag ggtgatcaag taccttatgg ataccggaaa aattttccat aacgaaaagg
    55621 gtatgctggc atggcaccat cgaaaggaag gcggattcga ggtagaggag tgagcaccta
    55681 agcgactatt ggcttcaact taaagttaag aagaatcaat taggcgtgaa aaacctagtt
    55741 ttgacacgaa aagtgttttt agtaagtatg ttaaaagtta attggataaa gaatactttt
    55801 aagccatcaa cggttgaaca agttccaccg ggaggcggct ttcaaattat actttattct
    55861 ttatccttta ccctctttaa tctggaatcc gccagtagtc ggacaagttc cgccagtagt
    55921 cggacaagnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    55981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntc cgccagtagt
    56041 cggacaagtt ccgccagtag tcggacaagc tctggaattt aacgtatatg gctgatttga
    56101 ttttaaatga tataaaattt ctaccgggtg tggggcctaa gcgcgccgaa ctactccaaa
    56161 aggagttgaa tatcagcaca tttggcgatt tgctctattt ctttcccttc aggcatattg
    56221 accggtctaa gatttataag atctcggaga taaatgagga tttaccctac atccaaatag
    56281 ttggtcgaat acttcggatt gaggaggttg gtgtggggcg caacaggcgg ctgacagcct
    56341 atctgagcga tggaagtgga agtgtagagc tggtatggtt tcggggtgtt tcatggatgg
    56401 caaagtggtt aaaacccaac atcgagtacc tggtatttgg caaggctaat gtatttggag
    56461 gtcatttcag cattgcccac cccgaaatgg aggagtacaa aaccggtcag gagaaaatta
    56521 tggggaccct gcaacccgtt tactccacca ccgaaaagct aaaaagcaac aggattacct
    56581 ccaaaaccat actgggactt cagcaaaccc ttattcaagc tgtcaatgga aaaattgagg
    56641 aaaccctacc tccctacctg attgagcagc aaaagctcat gacacttccc gaggctctca
    56701 ggaacattca ttttccccaa agcgtcttac ttttgaggaa agccgaacat cggcttaagt
    56761 ttgaggagct tttcttcata cagctcgata tccttagaaa acaaaccata cggcaaagca
    56821 agatcaacgg gcatttgttt tcgcgagttg gtgaaatgtt caaccgtttt tacagcgaaa
    56881 ggctgccatt ccctttaacc aatgctcaga aacgcgttat tcgtgaaatt cgtcgcgata
    56941 tgggcagtgg aaagcaaatg aataggcttt tacaaggaga tgttggtagc ggcaaaacca
    57001 tagttgcatt gatgtccatg cttattgctg tcgataatgg ttttcaggca tgcctcatgg
    57061 cacctactga gattttagcc aaccagcact ttgactccat tacctctctt ttaggagact
    57121 tacccgttac tgttgggctt cttacgggca gcacccgcca gtcggaacgc aacacgcttc
    57181 ataaatccct tgaggcaggt gaactgcaga taattattgg cactcatgcg cttattgagg
    57241 acgttgtaaa gttcaaaaac cttggttttg tggtgattga tgagcagcaa cggtttggtg
    57301 ttgcccaaag ggcaaagcta tgggagaaaa acaataatcc accccatgtg ctggttatga
    57361 ctgccacccc aattccgaga accttggcca tgacagttta cggtgatttg gatgttagcg
    57421 tgattgatga gctgccacct ggccgaaagc ctgttaaaac gctgtactac accgatttga
    57481 agcgaaatgt ggtttttggc ctgatgcgcc agcagctgaa ggagggccgg caggtttacg
    57541 tggtttaccc gctgataaag gaatcggaaa aaatggaaaa cctgaaagac cttcaggatg
    57601 gctatgaaag catcgtacgg gcatttccgc cccccgaata cgttacagtt gtggttcatg
    57661 gccagatgaa acccgaggaa aaggaatcct ccatggacat ttttgttaaa ggcaaggcca
    57721 acatcatggt ggcaacaact gttatcgagg taggggttaa tgttcccaat gcaaccgtta
    57781 tggtaattga gagtgccgag aggtttggcc tgtcgcagct gcaccagctg cgcggtaggg
    57841 tagggcgggg agccgacgaa agctactgca tacttgtctc atccttaaaa atttcttctg
    57901 aatcgcgtaa gaggctacag attatggtta ataccagcaa tggttttgaa attgccgaaa
    57961 tggacctcaa acttcgcggg cccggcgaca ttgaagggac tcttcaaagc ggaattccct
    58021 ttgacctgaa aatagccaat cttagcaccg atggaaatct acttcagcat gcaagggctg
    58081 ttgccaggga tgtacttaat gatgacccgc tattagagca tccccggaat gcacttcttt
    58141 cacaacggct tgatgaactt gccaactcac ggagtaactt cggaagaatt agctagcagc
    58201 taatcgttac gcgggaaaat tttagcttag tagtaatttt tatcgtatta tttttggggt
    58261 gtaatgaacc ttcatttcca atatactttt tagtgttgaa gtaactagat gttcataatc
    58321 tagcttacaa taaaatagtt gattgctgat aataagaatc gtaatgaaac acaaacccat
    58381 catacatgtt gtttttactc ttttgctttt ggttgtcagc gaggctaccc tatcgcaatc
    58441 ggtcaatccc tgtggaattg tcaacaggtc ttttgcatcg ggtgagaagt tggtctttga
    58501 gataagctat acatggcttt ttgtatggac agatgtagga atggccgagc ttacagtcag
    58561 cgccgagaaa cgttttggca gagatttact gcacattaag gcttcgggga aaacgcatac
    58621 attctacaac tggtttttcg aggtccacga cctttacgaa acatgggtcg atccggttac
    58681 tgttgagccg gtttacttta accgcgacat atatgagggc ggattcacca aggagaacga
    58741 gtacttctat gattggaaca aaaaccaggt agccataagg gtacgtcgta aaaaagggcc
    58801 taaccgatac gataccttga gtgttagcag gtgcacttac gatgtgattt cggctgttta
    58861 tgcagctaga aatttggact tctcaggtat taagccgggt aaaacatttc ctgttgaagt
    58921 tttgatggat gaggaggttt accatatcgg gtacaggttt cttggacgcg aagaaaaacg
    58981 ggtttcaggg cttggacgtt tcaggtgcct taaatttcaa attgacgtgg tagccggcga
    59041 catcttttcg ggcgaccaaa agctctacct gtgggttacc gatgatgcaa acaagctgcc
    59101 tgttgcggtg gaaactccaa tccaggtggg aagcataaag gccaggctta acacatacga
    59161 gggattaagg catccgttat catcaaaaat tccctaagtt gtatgggtat gacaactatt
    59221 ttccataaat tagcggcata atcaaaaaat aaatgccatg tcaagagcta tttccatact
    59281 aatcacattt ttactgcttt ttaccataga atcatgcttg gataacgaaa acacagtccg
    59341 atatactgat gttattcaga tggaagcagg tcccgttccc gataccatgg tagttaacga
    59401 aacctattct atacaattca gaatgggcgt tccaaattcc tgctggcatt cgctcgctct
    59461 caaccaggaa gagtttaacg attcaaccta ccggttttgg gccactgcgg tttacgaaaa
    59521 tcatggggaa aattgtgctc aggttgtagt aaccaaggat accgtaatag cctttaaacc
    59581 cacattggct aagccccata tattggtatt cttcaacgat cccgccagtg atccaagagt
    59641 tgatacggtt gtggttacgc ccaactaacc ccaaaaaatg ctggcataca cgccctgccg
    59701 tgataggttg gcttgcgggt ttttcagctg caaaaactgt tgagtggcac atttactttt
    59761 tgttgtgaag gatgttgctg gttttgccat catactaatt tgttaaacca ctcatattta
    59821 ttattttacc gttttgatgg ttcattttag ttaacccggg ttactacata ccgttaccta
    59881 tgaagaaaat actggttacc ggaggtgccg gatttattgg gtcacacctc tgtgaacgtt
    59941 tactgaatga gggaaacgag gtggtttgcc ttgataacta cttttccggg aaaaaggaaa
    60001 atgtggttgc cctgatgtca aacccatact ttgagctggt tcgccacgat gtaaccatgc
    60061 ccttttttgc cgaggtagat gaaatttaca accttgcctg tcctgcttcc cccatttact
    60121 accagtataa ccctattaaa acagtaaaaa catccgtgat gggcgccatc aacatgcttg
    60181 ggctggctaa acgggtaaag gcaaaaattc ttcaggcttc aaccagtgaa gtttacggcg
    60241 atccgctcgt gcacccccag ccggaaacat actggggcaa tgtgaatcct atcggcgctc
    60301 gctccagcta cgacgagggg aaaaggtgtg ccgaaacgct ttttgttaac taccactccc
    60361 agaacggggt taaaattaaa attgcccgta tttttaacac ctacggccca cgaatgagcg
    60421 taaacgatgg gcgggtaatc tccaacttta tagtccaggc tctcagaaat gaggatataa
    60481 ccatttacgg ggacgggaag caaacccgta gcttcatgta tgttgacgat ttggttgacg
    60541 cacttattcg gctgatggat tctcccgatg agtttaccgg tcctgttaac ctgggaaatc
    60601 cctgtgagta ctccatgctt gagctggcca gtaaggtgat tgagcttacc aaaagcaggt
    60661 cgaagctggt ttacttccca ctgcctgaag atgatcctct tcagcgtaaa ccggacatca
    60721 gcctggctaa aaaatttctt ggcggttggg aaccaaaggt tatgctggag gatggactta
    60781 aaaagacaat tgattcgttt gagcatatac taattagata gaaggatgaa gggaataatt
    60841 ttggcgggcg gtgccggtac tcgtttgtac ccaataacca aaagtatatc caagcagata
    60901 ataccgattt acgacaagcc catgatttac tatcccttgt cggtgctgat gcttgcccag
    60961 attagggata tcctgattat ttcaactccc aaggatttgc acctctacca ggatttgctg
    61021 ggcgatggtg ctcagctggg aatttcaatc agctatgccg ggcagccctc acccgatggg
    61081 cttgcacagg ctttcatcat tggcgaaaac tttattggct ccgacagcgt ttgcctggtc
    61141 ctgggcgata atattttcta tggcggaaac cttactgaaa tgctcctcga aagtgccaaa
    61201 cttcaggatg gagctattgt ttttggctac aacgttaagg atccccagcg ctatggtgtt
    61261 gtgggtttcg atagcgacgg aaaagtgaat agcctggagg aaaaacccac caacccaaag
    61321 tcgaactacg ccgtaacagg gctttacttc tacagcaacg atgtggtggc caaagctaaa
    61381 ggtttaaaac cttcaaaacg gggcgagctg gaaataaccg acctgaacag gctttacctc
    61441 gaagagggca ggctaaaggt taagctgatg ggacgcggta tggcctggct cgacaccggt
    61501 acccacgaaa gcctgctgca agcctccatt tttattcaga ccattgagga acgccagggg
    61561 cttaaaattg cctgtctgga ggaaatagct tatcgcaacg gctacatcaa cctggagcaa
    61621 ctccggaaat tggcgacgcc tctggagaac aaccagtacg gtgagtacct caagaggctg
    61681 gcttctggta atttttccta accgctaacc cgtttctttt ttaacgaaag ctgataagtg
    61741 atgctcacag tcgaaaaaac ctcaataccc gatctactga tacttaaacc caatattttt
    61801 gaggattcca gaggatattt ttttgaaagc tttaacgcca agctatttga tgattttgcc
    61861 attacttcta attttgttca ggacaacgag tcgttttcga agtatggcgt catcaggggg
    61921 ttacactacc agctgtcacc ctactcccaa acaaagctgg taagagtggt ttacggaaaa
    61981 attttggata ttgctctgga tataaggaga aattctccca cttttggcag gtatgtagct
    62041 ctagaaatct ctgctgagaa caaggcacag ttttacattc caaaaggatt tgcacatggt
    62101 ttttcagtgc tgagcgattc agctatagtt tgctacaagt gcgatgccta ctacagtaaa
    62161 gaagcggaga ggggaatacg cttcaacgat ccgggtttga agattgactg gggcattccc
    62221 gatggtagca tggttgtttc ggaaaaggac ctcaagcttc cctttttctc tgaagcagat
    62281 attaatttta attatggcag catcacctag cagggttttg gttacaggtg caaacgggca
    62341 gcttggaacc gctcttcgta aacgggcaac caagccggcg aatcatctcg tagctacatt
    62401 tacagacttt aacgagctgg atataaccag cgaccaacag gttgacaatt tctttcagca
    62461 aaaccatttt gattaccttg taaattgtgc tgcctacact gcggttgatc gtgccgagga
    62521 tgagcctgaa aaggcatttc tgattaacgg ttatgcgccc gggttgctgg ccgaggcatg
    62581 caagagccat ggaaccaaac tctttcacat atccaccgac tacgttttcg atggaatgtc
    62641 caactcgccc taccatgaat cttccccggt caatccgctt tcggtttatg gcaaaagcaa
    62701 gctcatgggc gaaaacaaag tgttagaatc gggagttggc atggtaattc gcacttcgtg
    62761 gttatactcc actacgggga acaactttgt taaaaccata ctcaggaatg cctcatcaaa
    62821 ggcacagctc aacgtggttt tcgaccaggt tggcacccca actcttgccg atgatttggc
    62881 agcagtattg ctccaaatta ttgaaaaggg cgatagctcc tttcgtcccg aaatatttca
    62941 ttactcgaat gaaggtgttt gcagctggta tgacttcgcc tatgaaattg tggcaattgc
    63001 cggcctacct tgtcgtataa atcccatcga aacaaatgat tacccaactc gtgccattcg
    63061 accccggtat agcgtgttga gtaaagaaaa aattcgtaac ttgctgccaa tcgaaattcc
    63121 ctactggagg gatagcctta aaactttcct aaaagaaatg ctataaccct aaaatgaaat
    63181 gccatgaatg ctgactcatc ggatagccaa gtgatggaaa gggcaaagca atggctaagc
    63241 cctgtttttg atatggaaac gagggagcaa gtgcagcagc ttattagtgg taaccacgac
    63301 gagctggttg agtcgtttta cagagatttg gaatttggta cgggaggact aagggggatt
    63361 atgggtgttg gtacaaaccg aatgaatata tacaccgtgg gcattgccac gcaagggctt
    63421 gcaaactatc tgaaaaagca acttcctggt attcctgagg ttagggctgc cattgccttt
    63481 gattcccgaa acaacagcat aaaatttgcc caaattgctg ccaaagtcct tagcgcaaat
    63541 ggggtgcggg tttacctttt cgattccctc aggcctactc ccgagctgag ttttgctatt
    63601 cgaacgctta aatgccattg tggcattgtt gtaaccgctt cgcataaccc caaggaatac
    63661 aacggataca aggtttactg ggaggatggc gctcaggttg ttccacctca cgactcagga
    63721 atcattgcag aagttcaggc aattaggaac atttcggagg tgaatttcaa cggcaacgaa
    63781 agcctgatta gcactatagg accggagatg gatgagattt actccaatac cctttcgtcg
    63841 ctttcgctct ctcccaaaat cattaaaaaa cataaggaaa tcgtcattgt ttatacccca
    63901 atacatggca ccggggtgaa gctggttccc aaaacccttc gtaagtttgg tttcaccaac
    63961 atagtgaatg ttcccgagca ggatgtggtt gacggcaact ttcccactgt ttcatcaccc
    64021 aaccccgagg aaccatcggc catgaggttg gcacttgaaa aagccaagga ggtaaatgct
    64081 gatgtggtca tggctaccga ccccgatgcc gacagggtag ggcttggagt tagaactcct
    64141 gaaggcaagt acacccttct caacggcaat caggctgcat caattcttat ttactacgta
    64201 cttcaaaaat ggcacgaagc taaccgaata aagggcaggg agtttatagt aaaaacgatt
    64261 gttacaaccg atttgctagc cgaaattgcc agaaaatacg gagtggagtg ctacgaggtg
    64321 cttaccggtt tcaagtacat tgctgaaaag ataaggcagc tggagggtga aaaaaagttt
    64381 attgccggtg gcgaggagag ctacggctac ctgtgcggtg attttgttcg cgataaggat
    64441 gccgttatca gctgcgcctt gtttgctgag gccactgcct gggctaagga acaggggaaa
    64501 tcgctctatg aggttcttgc tggtatttac agggaattcg gactttacag cgaaaagcag
    64561 ctctccatca cccttaaagg caaggagggg ttggaggaaa tatccagaat gatggttggc
    64621 ttcaggaaaa acccaccgct gcaaattgat ggcttgccag ttattaccat ccacgactac
    64681 ctgtcccagg aaaccaaaga tgtcccgagc ggttcgcgta ggcctttaca acttcccaaa
    64741 tcggatgtgc tgcaattcct tactgccgat ggaacaaagg tttcggttcg tccttcgggc
    64801 accgagccca aaataaagtt ctacattgcg attaggggcg attttaaccc caatgctggc
    64861 attgacgagc agcttgcgct tttggatgaa agaatttcac ggattgctgc cgagctaaag
    64921 attacccggt aattttgcct atgtgcaggt taaatcttat ttatgaagtc gtacagaaag
    64981 gaattatggt ttgaaacacg caacaggcgt gagctgatta acattaccgg ggaagtggag
    65041 aagtgcctgg ctgagagcgg tatttccgag gggctggcgt tggtcaacgc catgcacatc
    65101 acttccagcg tattcataaa tgatgatgag agcgggcttc atcacgattt tgagatttgg
    65161 cttgagaagc tggctccgga aaaaccatac gcccagtacc ggcacaacgg ttttgaggat
    65221 aatgccgatg cacatttaaa gcgaaccatc atgggacgcg aggtggtggt agccataacc
    65281 aatggtaagt tcgattttgg accctgggag caaatttttt acggcgagtt tgacggtaaa
    65341 cgacgaaaaa gggtgctggt taagatgatt ggggagtaat ccagattaca gctctttgat
    65401 ttccgaagtg tcctttagcc gctattttcg ttttcccttc atttccatca aaacccaaac
    65461 ggtgtaccgc tcaccatcct tcatgcaagc cttttttacc attttaacct ccgaaagggt
    65521 ggcgttaaca atttttcttt gggtaacccc cttatccgat ttaatatttt caacctttac
    65581 tgcaccagct tgtgtggcga tagcggtttt ggcatccatt tttgcctttt cctcggccat
    65641 ttccggcgta gtagatgttc caacaccaac accatagaat tttcttaccg attttggtgg
    65701 gttattaacc cattcaggcg ctgaacctac aaaatcctgt gctgaggcag ctagggctgt
    65761 cgttagtagg agtataacca ttagtttctt tttcattccg gttaatatta aaggttataa
    65821 gccatttact agttgtacaa ccactaattt tttttaaaat ttactcaaac ctatttccga
    65881 tttttataca tcacattttg tgccattgca ttgtgccagg ctctagtttc cccaatcctt
    65941 ggaaaatttt aattgctcgg gtaggtagga tgctccatgc gttgagcttt caaaatttag
    66001 ctatttttac cgttcgattt ttttttgatc tagcctatgg atagcgtaag agaactgtcg
    66061 gccaagtaca gtccggccgg aattgaggat aagtggtaca ggtactggtt agaaaagggt
    66121 ttctttagct caacgcccga tgagcgtgaa ccctactgca ttgtaattcc tccaccaaat
    66181 gttacgggtg ttttgcacat ggggcacatg ctcaacaaca cccttcagga tgttctcatc
    66241 cggcgggcga ggttgatggg taagaatgcc tgctgggtgc cgggtaccga ccatgcctca
    66301 attgccaccg aggcaaaagt tgtggctaag ctgaaagccg atggaattga aaaatcacag
    66361 ctaagccgtg aggagttcct taaacatgcg tgggagtgga aggaaaaaca cggggggatt
    66421 attcttgagc agctgaaaaa gttgggggcc tcatgcgact ggtcccgcac ccgctttacc
    66481 atggacgaaa ccctttcggc tagtgttatc catgtttttg ttgacctttt caataaggga
    66541 tacatatacc gcggcgttcg catggtgaac tgggacccgc aggcaaaaac ggcggtttca
    66601 gacgaggagg tggtttaccg cgaagtgaag ggaaagcttt acttcgtccg ctacaggatt
    66661 gagggtgaag attcgtttgt tactattgca accacaagac ccgaaaccat tctgggtgat
    66721 acagcccttt gtgttcaccc cgatgacccc cgatacaccc atctgaaagg taaaagggtg
    66781 attgttccgc tggtaaaccg tagcgttcca attatttacg atgattatgt tgacagggag
    66841 tttggcaccg gcgctctcaa gattactccc gctcacgatg taaacgacta cctgattggt
    66901 caaaaacata acctggattc tattgacata ttcaacgatg acggcacaat tagttccgca
    66961 gcaaaactat acgtgggttt ggatagattt gaggtgcgaa ggagaattgt tgatgattta
    67021 gaaagctcgg gaaaccttga acgggtagat gactatgtaa acagcgttgg ctttagcgag
    67081 cgaaccgatg ccataattga gcctaagctt tcgatgcagt ggttccttcg catgaaggag
    67141 ttggctgaac cagccctcaa ggctgttgtg gagggcgata ttcagctcta tcctcctaag
    67201 tttgtcaaca cctaccgtca ctggatggaa aatgtaaagg attggtgtct ttcacgccag
    67261 ctttggtggg ggcatagaat tcctgcatgg taccttccca atgggggctt tgtggttgct
    67321 cataatgttg aggaagcggt agagctggca cggaaagcag ccggtaattc cggtctagtg
    67381 gccgacgatc ttagacagga cgaggatgtg ctggatacat ggtttagctc gtggctctgg
    67441 ccaatctcgg ttttcaatgg tattcttgag ccaaacaacc ccgacatcag gtactactac
    67501 ccaaccaacg acctggttac cgcacccgaa atacttttct tctgggttgc ccgaatgatt
    67561 atggccggtt acgagtacat gggcgataaa cctttccgga atgtttacct caccgggatt
    67621 gttcgtgatc acctgcatcg caaaatgagc aagcagctcg gaaattcacc cgatccgcta
    67681 aagttgatcg agaagtacgg ggccgatggg gttagggtgg gaatgctttt atgctcacct
    67741 gccggaaacg acctgctttt cgacgaaaac ctgactgagc agggtcgtaa cttctgcaac
    67801 aaaatatgga acgccttccg gttggttaag ggctggcagg ttgacgaatc tgcagttcaa
    67861 ccgcagtcgg ctattgctgc cattgaatgg tttaactccc tgctgaacaa gtcaattgag
    67921 caggctgatg atgactttgc aaagtaccgc atttcggaag cactcatgaa cctctacaag
    67981 cttttctggg atgattttgc gtcatggtac ctcgaaatta ttaaacctgc atatggtaaa
    68041 cccgtagatg ccgttaccta taacgcaaca gtaagcatat tcgagaagtt gctgattatg
    68101 cttcatccat tcatgccatt tattaccgag gagctttggc aacacatacg ccagcgaggg
    68161 gatggagaaa gcattatggt tcaacaatta ccccattcaa caggatttaa ccaggatatt
    68221 ctaatcggtt tcgatagggt gaaagaggtt gtcgtgggcg taaggaatat acgccaggaa
    68281 aacaacattt cacccaagca acccctacag ctgagcatac gaaatgccgc taagctcgat
    68341 gctggtcttg aggagatcat ttctaagctg gcctcgataa actcgttgac ctacattgac
    68401 cagaaaattg agggtgcagc tacgttcatg gtgggcacta cggagattta catcccgcta
    68461 ggagggttaa ttaacaccga ggaggaacgg gaaaaaatac tttccgagct cgagtacacc
    68521 aaaggatttc tcgaaaaagt aatgaaaaag cttaacaacg aacgttttac aagcaatgcg
    68581 cctaaagccg ttgttgagtt ggaacacaaa aaacaagccg atgccctggc aaagattgaa
    68641 gctctacagg aacgcttgaa gtcattgggg tagtgccctc gaactttaga atattactaa
    68701 ttcatagcgc aatcattctc tgcctgtaac aagctgtaat gatttacagc tttgagtgga
    68761 atatcatttt ggaatattca tccaaaatgc tcacctcttt ttttgaattt acttctatct
    68821 gccctttaaa atgtaaagtt tggttacggt ttttccattt tgagcctgcg gatttgaaat
    68881 tatgttatga aacatccgaa atcctttaaa tctgtaatct gtaaaatcgt aattttgtaa
    68941 aatattaatc taagaccggt taaaatgaac gattctgaaa ttttgatgaa ccccaatgag
    69001 ctggttcggt ttcttaaaaa accttcagct gagtttacca agaatgacat catccgcttt
    69061 tgtgaggcta agaatattga gatggtcaac tttcgctatg tggccgacga tggcaggtta
    69121 aaaaccctga actttgccat ctcctcaaag gagtatctgg atactattct ttcggctggc
    69181 gaaagggttg atggtagcag cttgttttcg ttcatcggtg ctggtagtag cgatctatac
    69241 gttattcccc gctaccggac agcctttgta aatcctttct ctgaagttcc agcaattgat
    69301 attctatgct ctttctacga tagcgagggc aaaccactcg aaagttcacc tgaaaatgtg
    69361 ctccggaaag ctcataatct ttttaagtct gagacaggtt gtacctttaa ggctcttgcc
    69421 gagttggagt actacgtaat tagcgagcgc gatgattttt tccccggaac agatcaaaag
    69481 ggctaccact catctgctcc ttttgcgaag tgggaggatc tccgaaagga ggctttaatc
    69541 tgcattactc gtgccggtgg tatggtgaag tatggccacg ccgaggtggg atgcttcatg
    69601 acggaggaca cactttatga gcagcacgag atagaatttc tgcccatgcc ggttgaggat
    69661 gccgttgacc agcttgtaat tgccaagtgg atggtgcgca tgcttggtta ccggtacggc
    69721 gttgatataa gttttgctcc aaaaatcact gtggggaagg ctgggagcgg cctccacttt
    69781 cacctgatgg tcgaaaagga tgggaaaaat ctggtgtttg aaaatggaag gataagcaat
    69841 acttcaaaaa aaatgattgc gggaattcta gacgcagccg acgccataac cgcttttggg
    69901 aacacaattc ctacttccta tttacggcta gtaccccatc aggaagcgcc aacaaatata
    69961 tgctggggtg agcgtaaccg ttcggtggtt atccgtattc cacttggctg ggttggcacc
    70021 gaacagatgg ttacccatgc aaatcctgcc gatacaagcg cacagccagc aggattgaac
    70081 aggcaaacct ttgagtacag ggtggccgat tgttcagccg atccctacct aaccgttgct
    70141 gcgctggtgg ttgcatcgct caatggccta cgtatgaaag atgccctgca gcttgccgat
    70201 aagctctacg tagatgtaaa tatttttagt cctgaataca aggagaagct tgccgcctta
    70261 aagcagcttc cggcttcctg ttgggaatcg gctgaagcgc tcgaggctaa aaggcatatc
    70321 tttgaagccg atggaatttt ccccaaaggg atgatagatt cccgaattaa caatttgaaa
    70381 tcattccagg ataagggcct tagcgaaaag atttatggta aaaccgaaga aataaggcag
    70441 ctggtggagc gatttatcca cattggatag aaaaaggcag aggacaggct cagacaagga
    70501 taaaggataa aataattcaa gaacttgtcc gcctac
//
LOCUS       DAOSHM010000005        68243 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_323_length_68243_cov_19.468895, whole genome shotgun sequence.
ACCESSION   DAOSHM010000005 DAOSHM010000000
VERSION     DAOSHM010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 68243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 68243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68243
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_323_length_68243_cov_19.468895"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2930
                     /locus_tag="PK839_01500"
     CDS             <1..2930
                     /locus_tag="PK839_01500"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOG71439.1"
                     /translation="VSGRVTDAETGETLPGVNVIVKGTTRGTTTDVNGNYKIELIAED
                     KILSFSFVGYEDVELEVLNQKVVDVALKVKSTLMQEVVVVGYGTQKAKDLTAPIVTVK
                     GNDLSKQVTSNPLNALQGKVSGVQIINSGVPGSGASIKIRGVGSVGDYANPLYVVDGV
                     FVDNIDFISPSDVEELTILKDASAAAIYGVRAANGVIIITTRKGKSGLPVVSYDGYVG
                     VQMPVNILKMASKNQYITLLNEANANIPGYIPKNPSDYPTSTDWYNELVRTAGINNHN
                     IDITGATDKTSYSIGGNFLYQNGIMDAQNNYQRYNIRGRLEQQVNKNLKIGINTIISN
                     YNKNIPNSDAFFGAYVNPPVYTVYDPTNAEAYPVKFGAPQKYGFGNQYGNPVAAAYYA
                     DNFEKGHKEVFSIFGEVDIVKDILSYRVAYNQDQDSWNQRYYTPQFYVGGSQGVTKSN
                     LTKTFGNSSKQIIDNLLTLKRTKGKTSYTVLFGQSTRIEKRETLSGYANSVPGYDDQS
                     KYLVNGSFRDRNAYDAAERYNGLSFFTRGTLNFREKYLATVTFRADASSKYQKKWGYF
                     PSVGFAWNISQEGFSKLNAAFSNLKLRLSWGMLGNDNVPSNSSVILGQTGIGSSGVFG
                     DNLVDGMGAQTVLQNYLRWETVTEFDVGIDYTLKNEKLSGEVDYYHRVTNNVVFYAPI
                     ATGGGVAELLGNNGKVLNTGLEFTLNWKDEISEDFNYFISFNATFNHNEVLELNGREY
                     IPSGYIRGNYTTRTAVGHPIGSFYGYEIDGVYKSEGDALKDPVSQAIKDKGYFKYKDQ
                     NGDMVIDDKDKVYLGSAIPWLISGLDMGINFKKFDISLSIAGQYGNKILNAKRMNRDV
                     FSDGNYDEDFYQNRWTSSNKSSEYPSAEAYNSSFIQQANDFFVENGFYIRIQNIQLGY
                     TTDKISFIPKFRVFITAQRPFTFFTYKGFTPEVGGSPISSGIDNNVYPMQAIYTLGLK
                     MMF"
     gene            2951..4438
                     /locus_tag="PK839_01505"
     CDS             2951..4438
                     /locus_tag="PK839_01505"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOG71440.1"
                     /translation="MKRTRLTLLLIAALLAISSCDDFLDIRQEATLPTTGIDYTKPEN
                     IFLSVSAAYSKLRSWDAHTFPYIGAFEIASDNADKGSTPEDNPEMKDIDNLNFQPTNG
                     LINGLWTGYFDIVSGANYAIHQMPLFVETLPSQTDRDYAIQCQGEAKAIRAYAYFNLT
                     RLFGRVPIVDTVLSSEQLASLKQASTTELYNFIENDLQEAIAVLPDGYTKEWGGRISK
                     YTAMAIKAKVHLYQAEWDSVASLTDKIIASGRFKLLDNFREVFSLDGENSKESLFEIQ
                     SSTLGKSSGDAAYLDYGYVQGPRGNSPANMQGWGFCTPSQNLIDFYADRGEVVRPATT
                     FLYRGTVTPEGDSIKSNCTNPVYNGKVYTPSSQNRWVYNGYGFDHNIRVIRYSDILLM
                     YAEALIQGAAVPLSSGLSADDAVNLVRFRAGLDNITGVTLLDIWNERRAELALEEDRY
                     FDLVRTNQAATVLASKGFVVGKHEHYPIPAAQMQLNPNLTQNTGY"
     gene            4484..4996
                     /locus_tag="PK839_01510"
     CDS             4484..4996
                     /locus_tag="PK839_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG71441.1"
                     /translation="MKNKFRIFGTLLLGVMVAGTLTFSSCSSDDDDDNNNNGKVDPGT
                     IAEANLVAYFPFDGNATEKIANLTPTQQPGVTYVAGRRGQAYQGADQAHLLYTIPDGS
                     KLKTLTSFSLAMWLKSPLVTGDPEPTIFEIGKSDDLFWGNLKFALNRLSATADSLQLK
                     TFFLKSDATT"
     gene            5009..5365
                     /locus_tag="PK839_01515"
     CDS             5009..5365
                     /locus_tag="PK839_01515"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG71442.1"
                     /translation="MSYSNPSFQINKWMHIVVQYDGTTSKFMIYKDGVKVNIPANIEN
                     RIDGENGPALGPLAFENVDKLNFGAWRTKSEGGLSDAWMGWYLGNIDEFRVYDKALTA
                     TEVKALYDAEVSQITE"
     gene            5883..7163
                     /locus_tag="PK839_01520"
     CDS             5883..7163
                     /locus_tag="PK839_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016272419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HOG71443.1"
                     /translation="MGGNIINGFKAKFYTRVDSSEKFPFIPDDDLLTLIQQQTFRYFW
                     DFGHPSCGMARERDNSGDIVTIGGSGFGLMAIPVAIERGFITRSEGIERIATMVNFLK
                     NADRFHGAWAHWYNGATGHVVPFSTYDNGGDLVETSYMAQALLTVRQYLSAADPAEAE
                     LINNINLLLDGIEWSWYNRDNLNMLSWHWSPNYGWAMNMFVRGWNEALITYIMAATSS
                     TFPISAEVYHNGWAGNGAIVNGRSFYGYTLPLGHDYGGPLFFAQYSFLGLNPQNLSDQ
                     YASYWTQNVNHTLINRQYCITNPRGYLLYSPDCWGLTASDDNNGYGAHEPLNDNGTIT
                     PTAALSSMPYTPEESMRVARFLYYTLGDKLWGDYGFYDAFNPTAGWWGKSYLAIDQGP
                     IIIMIENYRTGLCWNLFMSAPEVDGALSKLGFSH"
     gene            7179..9410
                     /gene="bglX"
                     /locus_tag="PK839_01525"
     CDS             7179..9410
                     /gene="bglX"
                     /locus_tag="PK839_01525"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HOG71444.1"
                     /translation="MRIRKLSEITLAAIMSYTILSCSKTTMPYSDVVDQKVDSILMLM
                     TLEEKIGQLNLVTSDMDVTGPFIRKGYEEDIAKGRVGAIFNAYGAGYTRKLQELAVNN
                     TRLRIPLLFGYDVIHGHKTIFPVPIGEASSWDLEAIERSARVAAEEASAQGLHWTFAP
                     MCDIARDPRWGRVVEGSGEDPLLGSLIAQARVRGFQGTDLSQTNTILACVKHFAAYGA
                     AQAGRDYHSVDMSERQLREVYLPPYKAAIDAGALSVMSSFNDLNGIPATASKYLMTDI
                     LRNEWGFQGFVVTDYTSIMELLHHGVAADTLDAARLALNAGIDMDMQSGFYNSALLKL
                     LNEKKINEEQINQAVRRILRIKFLLGLFDDPYRYCSISREKETLLSNDNLLAARDMAK
                     KSIVLLKNQNNLLPLDKNIKTIAVVGPLANSQRDMMGSWSAAGDWPNSVTLLEGIKNK
                     IPGSQILYAQGCAIDDSNTGMIDEAVKIAKRADVVILAVGEAAWMTGEASSRASLDLP
                     GVQQELVERIYSTGKPIVAVLMNGRPLTIGWIDQHIPSILETWFLGTQAGNAIADVIF
                     GDYNPSGKLPITFPRSVGQIPIYYSMRSTGRPFDANNKYTSKYLDESNDPLYPFGYGL
                     SYTTFDYGEVELSSSEITETDTLDISVSVTNTGKYYGREMVQLYIHDNVASVAPPVKL
                     LKQFQFVDLKPGESKKMVFKLTVNDLMFYRDDMSYGFEKGQFTVYIGGNSRDVRTAFF
                     SLN"
     gene            complement(9558..10265)
                     /locus_tag="PK839_01530"
     CDS             complement(9558..10265)
                     /locus_tag="PK839_01530"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOG71445.1"
                     /translation="MATYIISDIHGCIQTLRMLIEGKLKVSLQDRLFFLGDYIDRGPD
                     SSAVVEYIIGLMRSGYTISCLIGNHEQMMLDSVKGESELCLWQANSGQATIKSYVKMF
                     GFDDDGHLGFLPKAHLDFFTSLKQYFIVDDKYILVHGGLNTTAANPLEDTEAMLWSRL
                     DDAPNDFMQDYRIIHGHTPEALDVIADKLKQPNRRIFGLDAGCVYRGMLFPGTGYLVA
                     LDLDKWELNFVECLDKW"
     gene            10550..10975
                     /locus_tag="PK839_01535"
     CDS             10550..10975
                     /locus_tag="PK839_01535"
                     /inference="COORDINATES: protein motif:HMM:NF016160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HOG71446.1"
                     /translation="MPSGNNVVEHLGRVDAITNNDIRVVITSQSACESCHARGACLAS
                     ESKEKFIVVTIPNHNFIVGEPVKVLLKESLGFRALFLGYVLPLLVVLTTLFTLSAAGM
                     SEKDAGLLSLAVLAPYYLGLYIFRKKIARKFEFGIEKIS"
     gene            10988..11854
                     /locus_tag="PK839_01540"
     CDS             10988..11854
                     /locus_tag="PK839_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010538335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster domain-containing protein"
                     /protein_id="HOG71447.1"
                     /translation="MSSVLTTSILMLTILGVVLAVILYVVAQKFKVEEDPRIDEVEAL
                     LPGANCGGCGYPGCRGLADALVKADDISAIFCPVGGNETMRKVAAILGKAVEDKDPQI
                     AVVRCSGSPQNRTRVVEYNGISSCKFASTLYSGDTGCQFGCLGLGDCVAVCKFDAIYI
                     DPVSLLPVIDEEKCTACGACVKACPKLLIELRKKGPKGKRIFVSCMNTDKGGVARKYC
                     SVACIGCFKCVKVCPYEAITLENNLAYIDANKCKLCRKCVEECPTDSILEVNFPPRKP
                     KVEEQAQNAVTS"
     gene            11897..13228
                     /gene="rsxC"
                     /locus_tag="PK839_01545"
     CDS             11897..13228
                     /gene="rsxC"
                     /locus_tag="PK839_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005641291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HOG71448.1"
                     /translation="MLKTFKKGGVHPPENKISAAQPVEVLPLPAKVIIPLSQHIGAPA
                     EAMVAKGDRVKTGQLIAKSAGFVSANIHSTVSGIVESLEPVMDSSGYKRPAITINVEG
                     DDWEPSVDRSPNLKKEITLSAQDIIKKIAEAGIVGLGGATFPTHVKLSVPAGKKAEML
                     VINGVECEPFLTSDHRLMLEKGHELMIGIQILMKALNVPKAMVGIEANKPDAVSHLQS
                     LAKSYSGIEVQPLKVKYPQGGEKQLIKALTGREVPSGGLPIDIGAVVHNVGTALAVYE
                     AVQKNKPLVERIVTVTGKNLAHPSNFLVRIGTPVSALIAAAGGLPDDTGKVVSGGPMM
                     GKAVSSIDVPVTKGTSGILVIPAAESKRKPEGVCIRCAKCVSVCPMGLEPYLLYRLGQ
                     RMQYSQMEVERVLDCMECGSCSFECPANLFLLDFIRLGKVEVGKIVRERKN"
     gene            13266..14270
                     /locus_tag="PK839_01550"
     CDS             13266..14270
                     /locus_tag="PK839_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010800186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HOG71449.1"
                     /translation="MDNRFVVSPSPHVHSGKSTSQLMLNVVLALMPAFAVSIYYFGVG
                     AIIVTLISVLSCVLFEYLIQKFIFKVKPTICDCSAVVTGLLLAFNVPSNLPWYIIVLG
                     SLFAIGVAKMSFGGLGNNIFNPAIAARIFLLISFPVQMTSWPLPVTSRLQYIDAITGP
                     TPLAIIKEGVKNGQTISQMADSIPSHMQLFMGLMGGSLGEIAAFALLIGFAWLLITRV
                     ITWHIPVSIFTTIFVFTGILWLINPGIYADPVFHLLTGGVMLGAIFMATDYVTSPMHP
                     KGMWIYGIGIGALTVIIRVWGSYPEGVSFAILIMNAFVPLINRFVKPKRFGAAAISGK
                     "
     gene            14295..14873
                     /locus_tag="PK839_01555"
     CDS             14295..14873
                     /locus_tag="PK839_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009017738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HOG71450.1"
                     /translation="MSGVESSFKNMTISLLAITLVASAGLGLVYKSTKDPIAAAQVAK
                     VNNAIKAVVPEFDNQPSNEAYTVKADGDDLFFYPASKNGQLVGVAVKTFSNNGFGGRI
                     ELMVGFLPDGTINKITVISHKETPGLGDKIDPGKSDFSVQFEGKNPETFKVMVKKDGG
                     DVDAITASTISSRAFCDAVNRAYKAFKEGGRK"
     gene            14870..15451
                     /locus_tag="PK839_01560"
     CDS             14870..15451
                     /locus_tag="PK839_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HOG71451.1"
                     /translation="MSAWSNLTRGIIRENPTFVIVLGMCPTLATTTSAINGLGMGLAT
                     TFVLLGSNVVISMIARFIPDKVRIPSYIVVIATFVTIVDLLMQAYTQSLYETLGIFIP
                     LIVVNCIVLGRAEAFARKNSVLDSAMDGLGMGIGFTMALTLLGSIREMLGNGSLFNYK
                     FIPGDAILVFILSPGAFIALAYLIALFNKFVKK"
     gene            15461..16036
                     /locus_tag="PK839_01565"
     CDS             15461..16036
                     /locus_tag="PK839_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex protein RnfA"
                     /protein_id="HOG71452.1"
                     /translation="MEYLIIIIAAIFVNNVVLSQFLGICPFLGVSSKINTSVGMGGAV
                     VFVMVLSTIVTYLLQQYVLNPLNIGYMQTIVFILVIAALVQMVEIILKKISPSLYQAL
                     GIFLPLISTNCAVLGTAILVIKHNYNLLEGVVFTAATAVGFGFALVIMAGIREQMELA
                     NIPKGMKGIPIALLTAGILSLAFMGFAGIVK"
     gene            16310..17122
                     /locus_tag="PK839_01570"
     CDS             16310..17122
                     /locus_tag="PK839_01570"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOG71453.1"
                     /translation="MRTGFIGQLLNYLRFRVNCKRRGGYGIHSPYVYSLYTETLNADS
                     KNNKVFERIEQYRKDLRRCGLSIVRQRLGDNPENGFPGDKVKLARIARNVSVPPHLGR
                     LLFRLCSRFNPEIIVELGTSVGISTMYMAAANPNSKVYTIEGEESILAIARNRFKQMG
                     FTNIEPIAGNFDVQLPELIKRISTIDFAFIDGDHNGRALLQNFNRMLPRVADTSVFVI
                     DDIRWSPGMEAAWKQICSNPKVTISIDLFRCGILFFRTGIAKQHFLLRYGPY"
     gene            17189..17920
                     /locus_tag="PK839_01575"
     CDS             17189..17920
                     /locus_tag="PK839_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG71454.1"
                     /translation="MSSTNLITLNEIKKFYQVGSVTVKALKGINLTIDQNEYVAIMGP
                     SGSGKSTLMNILGCLDTPTDGTYILNGTDVSRLSDDELADIRNKEIGFVFQTFNLLPR
                     YTSLDNVILPLIYAGVPKHERVEKAKQVLYNVGLGDRMEHKPNELSGGQRQRVAVARA
                     LVNNPSIILADEPTGNLDSKTSIDIMNLFEDIYSQGNTIIIVTHEEDIARHARRIVRI
                     LDGMIESDEVNPHPTLRQKVVLTEQ"
     gene            complement(18057..20144)
                     /locus_tag="PK839_01580"
     CDS             complement(18057..20144)
                     /locus_tag="PK839_01580"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018108199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HOG71455.1"
                     /translation="MQTKKILMALALPALVLASCKTQQPNNEAIVGPVKPVVTDSTLT
                     PEILWSFGRIGEFAVSPDGQKVAYTTAWYSIDQNRGNSEIYIMNTDGTGKRQLTHTNG
                     SEFNLQWVDSLNLAYLATESGKSQIWQMNVENQKTSMVEGFENDISGFSISPTGSKIL
                     LTSDVKVGASIADIYPDLPKSSGQIYTTLMYRHWDTWEDSLFNHVMVANFDGKSLSKI
                     IDIMANEPYDTPLKPFDGMEDIAWSPDGNQIAYTCKKLTGKEAAFSTNSDIYLYDLRN
                     GKTQNLTEGLPGYDKQPVFSPDGEKIAWLSMERAGFEADKDRLLVMDLQTKKTSYLTR
                     DFDYSVSNVIWNASSDMVYFIAGVEATYQIYRLALQDAKPVAITHGWHDYHAFALAGD
                     RLIGSKASIVKPVEIYSINPQNGEEVELSFTNQELLSQLKMPTVRQRWIKSTDGKLVH
                     TWIILPPDFDSTKSYPALLYCEGGPQSAVSQFWSYRWNFSIMASGGYVVVAPSRRGTL
                     TFGQKWTDEISKNHGGQDMKDLLSAIDEVKKEPWVDENRLGAVGASYGGYSVFWLAGN
                     HNKRFKAFIAHCGVFHSEMEYLTTEEMFFDTWEMGGAPWEKNNPVAMKSYAQSPHNFV
                     TSWDTPILIISGGNDFRIPYTQGMAAFNAAQILGVPSKFIFFPDENHWVLKPQNGILW
                     QREFRSWLDRWLK"
     gene            complement(20209..23559)
                     /locus_tag="PK839_01585"
     CDS             complement(20209..23559)
                     /locus_tag="PK839_01585"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOG71456.1"
                     /translation="MAKKRDLLRTSFILLLVLISQVNAIWAQTTGRFPVMNFHHREYN
                     GHAQSWAITQDNRGLIYIANNVGVIEYDGSEWRHISINGALARCLDTDKNGRIWVGGQ
                     DEFGYLAADSTNTLSFHSLSQHIPETAKPLGMIRQLYASDSSIYFSSFTTLFRLNKDL
                     TVSEWSPKTQFHRSYLTDGVLFIHQRDYGLCYLSNDSLRLVPGGDYFAPQLIYAILPF
                     NQKYFLICTQNDGFYLLSRQALDPNFTGYTKPAIVKFSTSDDNFFASSKVYAGLKLDD
                     NTFAISTYSGGVVFIDTTGNITRRIDTSVGLQDNTAWYLYMDNQKNLWIALNNGISYT
                     PIFSQLTSWTENDGIKGVLQSVVRFKNHIYFTTNVGIFWFSNGRIEQVTGLSDLSWKL
                     RTIHTMNGDEALLATTSSGIFQVEGGKATLIENGKYHSYCLLQSKLYPKITYVGLREG
                     VGVIAENNGRFKFLGELTGTKGEVYSLAEDLNGDLWYSERYKGINYVDMVNPYQLVSE
                     KPILYTLPFNPRYDDIAVVMIDGEVKASAERGLSKFSHQSNMFEPDSSLGLEFANGTT
                     GLRILNQDQNGNIWFEAYNFSANRWLERAVKYPKNIYRREPAQFRTIPETIFYDIFPE
                     DNNKVVWIACTDGLFRFDEDAGNLKNPIFRVLIRQVSAGPDHILFNGVYYDGNEGITF
                     PTSLPYNEVKSTTLPYRNNSLTFTYSAPFFGQQQKLLYSYMLVGFDKTWSDWQPNQRK
                     EYTNLPFGTYQFNVKAKNLFNIESPAETFQFTIQKPLYLQWWAITGYVVALLLIIWLS
                     IRINTQLLRNSNIKLQQLVDERTLELSKSEQALIEKNVELQRQKEEILTQRDELEEHN
                     RHINDSIIYAQTIQQAILPDLSQSLGNIFEHFLIYKPKELVSGDFYWISHAGPKSNPN
                     EKIFIAVVDCTGHGVPGAFMSLIGSRLLSEIVNEKKIYNPADILTELSASIKVALHQN
                     VSESFDGMDIALCLIESKPNDMFSVTFAGANRPIFCYSKAENRIQTLKGNRKSIGGLL
                     PDVDLEFTNWRVPMHKGDMIIMYTDGLTDQNNSSKRKFTTARFQTYLLANIEKPMEEI
                     SSVLLQEFNEFTGSEPQRDDITILGIRL"
     gene            23696..23827
                     /locus_tag="PK839_01590"
     CDS             23696..23827
                     /locus_tag="PK839_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG71457.1"
                     /translation="MNECIKATIRKVGSIEERWLHITLATGAMFFQCWNLFWQRDGM"
     gene            complement(23858..24835)
                     /locus_tag="PK839_01595"
     CDS             complement(23858..24835)
                     /locus_tag="PK839_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG71458.1"
                     /translation="MKRYLATLCLATTCTIVLAQSRSELMSREKIQKNKIASVSQWTH
                     KFNKDKVDPKGYVTVVTNYDKNGNEVEIINYKQNGSVSSRHTYKYDNQNRKIEYLQYQ
                     DYRQKGIELAFRQVFTYDKNGNKASELGFDGRTTYRIAYSYFPNGKQKEIIRYNAANQ
                     VEEKWIYEHNGNNTKISIFKPVGTLSKITERKYDSAGNLLDEKNTSGDGKELARTVFT
                     YNANNLPISKSEYYAGELRASYSYVYDGQNQLIETYLIKPNNTRLLYSSYKYDGQGNI
                     VEEKWYEDNATDYSHRNYKLDSKGIVDEVDTYYSDYNFRVVYRYEYKFF"
     gene            complement(25064..25651)
                     /locus_tag="PK839_01600"
     CDS             complement(25064..25651)
                     /locus_tag="PK839_01600"
                     /inference="COORDINATES: protein motif:HMM:NF038262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SiaB family protein kinase"
                     /protein_id="HOG71459.1"
                     /translation="MDNIIYFIKSTLASEIASSGFPLIYKGEMNHQIMRSFAFMANRK
                     ISDMDVPTSIRKRVFHIMIECLQNITKHSDDYDEKERQIGNGLFVVGREKDSFFIVTG
                     NLIRNDKMAPLEERLISLNNASPTELKSLFLQQMIGGELTEKGGAGLGLIDIARKSKR
                     KLYYHFVPYENNRHFFILAVTIPIEIDEDDNDENL"
     gene            complement(25782..26771)
                     /locus_tag="PK839_01605"
     CDS             complement(25782..26771)
                     /locus_tag="PK839_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HOG71460.1"
                     /translation="MENKGLYPLRFKPIFKPRIWGGNKLRQRLNKNIPKNISNCGESW
                     EVSAVGDNISVIANGFLEGNNLEELVEIYMGDLVGNSVYEKFGIELPLLVKFIDASDN
                     LSIQVHPNDELARERHHAYGKTEMWYVIDADPGAKIITGFNRDVTREEYLESLSTGKL
                     TDLLNVETALAGDVYYIPAGRVHAIGRGVLLLEIQQTSDITYRIYDWDRLDETGKPRE
                     LHTSLALDAIDFSKVSNPKRNITESLNTPIELANCPYFTVNKLTLTQPLKRDYFMIDS
                     FVLYICLAGDVLVNYPNGKEKIATGETMLIPAEIKNISLQPVSTCTIIEVFVG"
     gene            26862..27485
                     /gene="udk"
                     /locus_tag="PK839_01610"
     CDS             26862..27485
                     /gene="udk"
                     /locus_tag="PK839_01610"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007759125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HOG71461.1"
                     /translation="MLIVGIAGGTGSGKTTVVNRIIDLLPKGEVVVLPQDAYYRDSSH
                     LPLEQRQEINFDHPDSIEFELLVEHVKLLKEGKAVEQPIYSYLTCTRAKETITIKPRH
                     VVIIEGILIFTCPELRNLLDIKVFVDADADDRLSRVIARDIVERGRSVNKVLDRYEKT
                     VKPMHLQFIEPTKRYADIIVPQGGNNAVAISILASIIEKTLRNHNLV"
     gene            27488..28210
                     /locus_tag="PK839_01615"
     CDS             27488..28210
                     /locus_tag="PK839_01615"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOG71462.1"
                     /translation="MLKDVSPENILFLDIETVPAYPAYTDLPEADRKLWDKKAAQLVK
                     DHETAADIYGRAGIYAEFGKIICISTGLVVSKAEERFFRIKSFFGIDEKILLADFAEM
                     LSRFEKRKDVNLCAHNGKEFDFPYIARRMLVNDIRIPDILDVSGKKPWEVRFLDTMEL
                     WKFGDNKHYTSLNLLTHIFGIPSPKDDIDGSQVAECYYLQNDLNRIATYCEKDTLAVA
                     QLFMRYKGLPQIAPENVERVNG"
     gene            complement(28207..29010)
                     /locus_tag="PK839_01620"
     CDS             complement(28207..29010)
                     /locus_tag="PK839_01620"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOG71463.1"
                     /translation="MKLFYRELGKGFPLVILHGLYGSSDNWMGIAKRLSTSHRIILPD
                     QRNHGQSPHSSSHTYPDMANDLAELVDNLGIDNFHLLGHSMGGRTAMLYQSLFPNMVK
                     SLVVADIAPWPYQPNDEVFRLSLAEHSSIIKGMLSLPLTSIKSRYEAEKMLTGSIPSQ
                     KLRQFLLKNLKRDASGSYSWCLNLPVLESSLRELITGVNLECGKKSPTRVLFVKGENS
                     NYLPENSEVRLTDYYPDCRLVTIRNATHWLHAEQPEAFTKSILEFLQKG"
     gene            complement(29010..29726)
                     /locus_tag="PK839_01625"
     CDS             complement(29010..29726)
                     /locus_tag="PK839_01625"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004297247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HOG71464.1"
                     /translation="MTRLSVNINKIATLRNARGGNIPDVLKAAIDCQRFGAEGITVHP
                     RPDERHIRYQDVRDIKPIITTEFNIEGYPVESFIELVLSVKPQQVTLVPDPPDAITSS
                     SGWDTINNKDLLKRMVATFKREGIRTSIFVNADPEMVKHAADTGTDRVELYTEPYASM
                     FKVNPEKAIEPFITAADAAKQVGLGLNAGHDLNLENLSYFNRHIPNLLEVSIGHALIS
                     DALYYGLENTIQLYLQQLRS"
     gene            29788..30453
                     /locus_tag="PK839_01630"
     CDS             29788..30453
                     /locus_tag="PK839_01630"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOG71465.1"
                     /translation="MIALEMISDVIPALKTSDTGQTALNWMDIFKVSHLPIVNNKEFL
                     GLVSESDIYDLNMPDEPLGNHYLSLNSPYVIEDQHIFEVMEVLSRLKLSLVPVLDSGK
                     NYLGVITLMELLHYFAVESGISLQGGIVVLEMNANDFSMSQVCQIVEGNDAKILAAYV
                     TPHPNSTRMELTLKLNVTDLTSIRQTFNRYNYTVKASFLKQDDEADMLNDRINFLFRY
                     LDI"
     gene            30472..31350
                     /locus_tag="PK839_01635"
     CDS             30472..31350
                     /locus_tag="PK839_01635"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF002521.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HOG71466.1"
                     /translation="MVVAIYGKAVETEYLPSLGELFNQLVEHNVEVWVYRPFMEYLLE
                     TFSAKLPDFEVFNTGSDINGKANILISIGGDGTFLDSVSFVRSSGLPIMGINFGRLGF
                     LANIAIPDMHNAIDLLIAGRFKVEHRSMLEVVSTLEQFNTFPFGLNDFTMQKASSGFL
                     KVNAYVNGEYLCAYWADGLIVSTPTGSTAYSLSVGGPIVSPNLSALILSAIAPHNLTV
                     RPLVISDDNILLLEAVGRDGSVMLSLDSRSITCKSPVTVTVKKAPFFANVVSMEGSNF
                     YHTLRGKLLWGMDKRN"
     gene            31434..32192
                     /locus_tag="PK839_01640"
     CDS             31434..32192
                     /locus_tag="PK839_01640"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HOG71467.1"
                     /translation="MKKSFIVVGIILFLCLPGHSQVWKRNRIEIFLGVPITHYFGDIG
                     GTSDPSSMMGIKDISIRAIRPGFSGGLGYRLTERFYAQGTLTLGLFGNSDKGSRNEAR
                     NYAFSTFGTELAAVAQFYIIPESDQNYFYSIMQVRGGLRKINKPFSLYLFAGGGGCFY
                     RVKPLNDLVDSDRFDDSQHTAVVVPFGLGFKYQIHSRMLLGVELGGRYVLSDYLDGFS
                     PEPSKYNDFYYVFNFKVHYRLPSNKILRKPQWTF"
     gene            32180..32857
                     /locus_tag="PK839_01645"
     CDS             32180..32857
                     /locus_tag="PK839_01645"
                     /inference="COORDINATES: protein motif:HMM:NF039345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HOG71468.1"
                     /translation="MDILANRIFFSFLSSLLVLSFFLLLPFKSHGQDKRDFGVQAGST
                     YYYGDFNEAKPLYQPSFAMGVIFRYNFNTQYSIRASALYGAIKGESPSGFFLPLSAST
                     TFSNTLLEAEVMGEFNFVSFSPLSTRKKKLSPYVNIGLGVGQLSGSVLFHIPFGVGLK
                     FSPGKRHTLAMEWRFHKTFNDELDSYAAPDDGKTPFLHNNDWFSYIGLIYTYRLYNSG
                     NVCPVYK"
     gene            32870..33607
                     /locus_tag="PK839_01650"
     CDS             32870..33607
                     /locus_tag="PK839_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005637341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HOG71469.1"
                     /translation="MDIKNQIDKNNVPQHVAIIMDGNGRWAEKRGLPRIFGHKNGVGA
                     VRAATEAAGEIGVKFLTLYAFSTENWKRPKIEIDTLMNLLIETIDNEIGNLNKNDVRL
                     ITIGNTSALPKNVQTKLKYAIDKTSQGKTLTTILALNYGARAEITEATRKILTEYRNG
                     KLDEMSLSPEVFGGYLQTAGIPDPELLIRTSGEIRLSNFLLWQLAYAELYFTPVLWPD
                     FSKDDFFNAIVEYQHRERRFGMTSEQL"
     gene            33745..36222
                     /gene="bamA"
                     /locus_tag="PK839_01655"
     CDS             33745..36222
                     /gene="bamA"
                     /locus_tag="PK839_01655"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HOG71470.1"
                     /translation="MLKSKIFLLLLLLSSVSALAQEPSFSYEVPKKYCVKEVRVSGIK
                     FLNPDVLVSVSGISVGDSILVPGDAVTKAIKKLWSQGLFSDIKISATKIENSDIYLDI
                     FLQEQPRISSINFVGVRKGEVSDLKEKLKLRNGSQLTESILENSVEIIRKHYRTKGFL
                     NVDVNPIQENDTALANMVKLTFDIRKNERVKIDQITFDGNTAFTDARLRKSLKKIHRR
                     DINIFKSAKFIQSDYDESKDNLIAFYNENGYRDSKILKDSVFTLNNKRIGINFLVHEG
                     PQYHIRSISWIGNTKLPSEALTAILGMKKGDVYDKTLLEKRLFTDDNSISTVYMDDGY
                     LFFNLDPVESQIQNDSVDLEMRIYEGDQATINKVLIVGNTKTNEHVIRRELWTKPGYL
                     FSKSEITRTIRELGQMGHFDPEKLDVQPMPNPAEGTVDLKYVVEEKANDQLEISGGWG
                     NKMFVGTVGIRFTNFSVRRLFDKEAWRPVPSGDSQSLSLRASTNGTYYKAFSFSFTEP
                     WLGGRKPTNFSFSVYHTIQNGGVDYFYQTSDKYFKVTGASVGIGTRLKWPDDYFSIYN
                     EISYQNYNLKDWTGYFLFSNGQSNNLSYKISLTRNSTDQVIYPRTGSNLSLSLQITPP
                     YSLFNGKNYSAFSGSEKYKWIEYHKWTGRIQWYVPLVEKLVLYSNFQFGIMGYFNSDI
                     GHSPFEGFDVGGDGMSGYNLYGKETIGLRGYKNGSLTPYTLIDGSYVGDGHVYDKYTV
                     EIRYPLTLNPQAAIYLLTFFEAGNAWRDASEFNPFNVHRSAGIGARMFLPMLGMLGID
                     WGYGFDKIPDNPDAHKGQFHFIIGMPF"
     gene            36409..36918
                     /locus_tag="PK839_01660"
     CDS             36409..36918
                     /locus_tag="PK839_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOG71471.1"
                     /translation="MKKILLLPIVLLISTSLWAQKFAFVDSEYILNKIPSYKAAQEQL
                     DKLSEQYQKEIEDKYAEIDKMYKDYQTEKVLLTQEMQKKREDEIISRERQTKELQMKY
                     FGRDGLLFKKREELVKPIQDQVFNAVKEIAIEGGYAIIFDAAASPNMLYTNPRYDKSE
                     DVLLKLGYK"
     gene            36957..37475
                     /locus_tag="PK839_01665"
     CDS             36957..37475
                     /locus_tag="PK839_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010266810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOG71472.1"
                     /translation="MRKISTIAFILFLSATGVKTYAQSYKFGHINSQEILSVMPERDS
                     AVAKLDKFTKTLQDQVEELQVEFNKKYQDYLQKRSTFTDAIREMKEKELTEMQQRAQE
                     FQQVAEQDYQRYQGEVMKPVIEKADSAIKKVAKANGFTYIFDTSTGVILYFSEQSVDI
                     GPLVKKELGITK"
     gene            37652..38473
                     /gene="murI"
                     /locus_tag="PK839_01670"
     CDS             37652..38473
                     /gene="murI"
                     /locus_tag="PK839_01670"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HOG71473.1"
                     /translation="MLGSNPIGIFDSGSGGLTVLYELLKVLPRESFVYFADSANCPYG
                     PKPQSEVIELSDCITRFLLEQGCKLVVVACNTATAAAIEHLRSNFKIPFIGMEPAIKP
                     ASLNSKTKSIGVLATAGTFKGKLYIETSKKYASDVNVCYQVGEGLVELVEAGKADSPE
                     AAELLHRYVDPMVDCNIDHLVLGCTHYPFLMNSLSRILPRGVEIVNPAPAVARQTLRV
                     LSENNLLNDGSSLSKVSFYSSASVEILKFLAEKLGVISNHGPAVNFTGGVKLCCR"
     gene            38514..40184
                     /locus_tag="PK839_01675"
     CDS             38514..40184
                     /locus_tag="PK839_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008763594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HOG71474.1"
                     /translation="MRREVEIVLENTNQKVRITPGLSLMELTGIFNIKLEHQVLGAMV
                     NNQLKELDYEIFTPKTIRFIDITHPAGMRMYQRSLFFLLHKAVGDILPGCKVRIEHSV
                     SKGFYCEIDGFEQPLDLPLVFSICDKMREVVAADIPFRRDKILTTDAVKLFEDLGYTE
                     QAKLFDTRPKLYTSIYYLGDLPGYFFGNLVPSTGYLKVFDLVKYYQGMLLRIPKRSKP
                     DEVEDVVIQNKMFDIFQEYKDWTHILGVGNVVAINEQVEQGNASELIKVSEALHEKKV
                     AYIADKIHSLGDKVKVILISGPSSSGKTTFAKRLAVQLRVNGIRPYTISLDNYFVDRD
                     RTPRDENGDFDFEALEALDIEYFNSDLLRLLNGETVELPKFSFEKGARFFDNTLMRID
                     PNGMIIIEGIHALNPKLTPLIPSDKKFKIYVSALTSMSYDGINRIPTTDNRLIRRIVR
                     DYRYRGYNALQTIARWESVRRGEDRNIFPYQEEADEMFNSALLFEFGLLKSYVEPLLQ
                     EVPPNRPEFDEADRLLKFLYYFNTISDSEVPPTSIIREFLGGSAFSYS"
     gene            40368..41741
                     /locus_tag="PK839_01680"
     CDS             40368..41741
                     /locus_tag="PK839_01680"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOG71475.1"
                     /translation="MARLFIVIINIIGITLAGILGGGNVLVTMKAPSRVDAGSEFDVE
                     VLLKKAGVDGFARFQQELPLGLTAELIESSLGDFTFEDQKVKVVWLKLPAAKELRFVY
                     RIRVDERLKGSFNLGGVFSYIENNERKTSSVQAAQISITPSTRINPSLIVDINEFQNV
                     IPVQAPVSMLASNVKCIRQTPIPTGEANDLLVKLLVNKGNAQKFAKIEEDIPQGFQAE
                     VVDAKDAIFTFKDQKVKFLWMSMPPDPRFIVSYRLIPINGNGEAVVSLSGKFSYLVGE
                     ATRVVDIIQKDVDLGNLDPKSIEGLLASIPGAEIAAPSSSSFSSSFTSAGSDGGIDIP
                     VQYQVIPEKSKKIAQPEQNMMLYMLEPEKGVYYRVQVAAGHKPINIKRYFLRHNVTYE
                     VRTEKHEGWYKYSIGSFKEYKEARDFRVQIWNTTKINDAFVAAYNNGVRITVQEALMI
                     TNQTWFR"
     gene            41760..43214
                     /locus_tag="PK839_01685"
     CDS             41760..43214
                     /locus_tag="PK839_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HOG71476.1"
                     /translation="MRRFIARNKITLFTLCISGMLLCTYNSAHAQDDEEYYKNLLDTV
                     VEVINPVYKPVLSFGAGIMSFWGDVQNPVTNPVNGKYGFKFNVSTLFGKNYFKGNIFA
                     IYGQFQGQNFDITRQMQLQPLPLDDIGNPIYPNSSFQTEFWQVGITAEYGFGHLFGLV
                     KRFKPFIAVGVSTMFFTPKGNVRYGPTGDFYYAWSDGSLRDFPENGPNAYLAQIIKLD
                     NSYETDLSSADLYRIGSYSQNTFTIPLDLGFDFYLSDRVNFRVGATVNYAFSDLLDNY
                     SSKTASTMGYPNKNSYNDIFAFTYFSMNLDLFSDPKTMLVERVFAELSGTFDYGVFFA
                     DQDGDNVLDRFDACPDTPPGVAVDSLGCPPDYDNDGVFDYMDDESSTPEGAIVNERGV
                     QLSADVLSDMFSKNSMAVPRSEVKVIPLANVWTRNATFTPGAIPDKFRIVDADADGYI
                     SFQELLKAINDFFDEKNTFTPDEIYELNEYFFSQ"
     gene            43457..43933
                     /gene="bcp"
                     /locus_tag="PK839_01690"
     CDS             43457..43933
                     /gene="bcp"
                     /locus_tag="PK839_01690"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HOG71477.1"
                     /translation="MKTTFLKPGDTAPDFTAVDQDGVEFSLSEYQGRAVVLYFYPKDN
                     TTGCTEEACSLRDGYTELKQLGLDVIGVSPDSTRSHRSFIQKHSLPFRLAADTDHRIA
                     ELYGVWAEKSMYGKTYMGIVRTTFIINGDRKITHVFDKVNTKAHAMQILEELKKTL"
     gene            43946..44977
                     /gene="recA"
                     /locus_tag="PK839_01695"
     CDS             43946..44977
                     /gene="recA"
                     /locus_tag="PK839_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005636551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HOG71478.1"
                     /translation="MEKVEKEKEKKERKEISQDKLKALQSTLDKIEKDFGKGSIMKMG
                     DKAVADVASISSGSIALDIALGIGGYPRGRVVEIFGPESSGKTTLAIHAIAEAQKLGG
                     IAAIIDAEHAFDRSYAEKLGVDVETLLISQPDNGEQALEIADNLIRSGAIDIIVIDSV
                     AALTPKAEIEGEMGDSKMGLQARLMSQALRKLTGTISKTNTCCIFINQLREKIGVMFG
                     NPETTTGGNALKFYASVRVDIRKLNQLKDGEESTGNRVRVKIVKNKLAPPFKKAEFDI
                     IFGEGISRTGEIVDLGVECNIIKKSGSWFSYGETKLGQGRDSVRQLLQDNPELASEIE
                     AKIRETLKG"
     gene            complement(45095..46645)
                     /locus_tag="PK839_01700"
     CDS             complement(45095..46645)
                     /locus_tag="PK839_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007563673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HOG71479.1"
                     /translation="MNTELVIDVQEAEIAIALLENKQLVELNKEKGNVQFSVGDIYLG
                     KVKKIMPGLNAAFVDVGYEKDAFLHYLDLGPQFKTLQQYVQAHYARRTKTIPVPKFKL
                     EPDIDKNGKITDVLSSGQAVLVQIAKEPISTKGPRLTSEISIAGRNLVLIPFSDKVSI
                     SQKISSNEERKRLKRLMESIIPKNYGVIVRTAAEGKKVAVLDAELRSLIKKWETATEK
                     LKTVSFPGQLASELKRTSAILRDMLNGTFNSIHVNDEIVFNDIHEYIETIAPEKEKIV
                     KLYTGNVPIFDQFGIEKQIKSLFGKTVSIKSGAYLIIEHTEALHVIDVNSGNRSKSGN
                     DQETNALDVNLAAADEIVRQLRLRDMGGIIVVDFIDMHQNENRNMLFERMKELMSKDR
                     AKHNILPLSKFGLMQITRQRVRPEMHINTTEKCPTCKGTGEVSPTVLLDEQIENQLST
                     LVTSHKLKSITLKLHPYIAAFLNKGFVSKRMRWSFRYHCRINVTPMSSYHFLEYHFFD
                     SKDTEIGI"
     gene            complement(46882..47169)
                     /locus_tag="PK839_01705"
     CDS             complement(46882..47169)
                     /locus_tag="PK839_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOG71480.1"
                     /translation="MTKADIVNEISKTTGIEKVEAQKVVEAFMEIVKDSLAKEKNVYL
                     RGFGSFIVKKRAQKTARNISKNTTIIIPEHYIPSFKPAKTFVNKVKNHVKK"
     gene            47305..48378
                     /gene="mutY"
                     /locus_tag="PK839_01710"
     CDS             47305..48378
                     /gene="mutY"
                     /locus_tag="PK839_01710"
                     /inference="COORDINATES: protein motif:HMM:TIGR01084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HOG71481.1"
                     /translation="MDFSALLLEWFSVHHRDLPWRNTSDPYHIWVSEVILQQTRVNQG
                     TEYYQRFIHIFPDIPALAEAPLDAVMKAWQGLGYYTRARNLHAGAKQVMSEFGGKLPT
                     SYSELLRIAGLGPYSAAAVASFAFNEAVPAVDGNVYRILARVFGVFTPVNSSQARREF
                     FNLAGELIDRNRPAEFNQAIIDLGALVCTPRRPSCSACPFSDYCYAYLNNLTGLLPLK
                     NRKNPPRSRYFTYLMINFNGTTFIRKREGNDIWHSLYEFPLIETPEQLDTTQLVETEA
                     WKELMGDSSAEIIHVSDPIKHQLSHITLHARFIIVQMRNPTYALRTGYRNISIGNLQE
                     LSVPRLIDSYMASEPAERYFTGK"
     gene            48442..48879
                     /gene="ssb"
                     /locus_tag="PK839_01715"
     CDS             48442..48879
                     /gene="ssb"
                     /locus_tag="PK839_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002561410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOG71482.1"
                     /translation="MSVNKVILVGNVGKDPDVRHLEGGVTVARFPLATNESYTDKSGK
                     KVTQTEWHNIVVWRGLADVAEKYVRSGKLLYVEGRIRTSSYDDKEGNKRYSTEIVCDN
                     FRFLGPNVNQQEHSTPYPEQVSDAKSNLSDVENIPSPDDDLPF"
     gene            49043..50350
                     /gene="gldE"
                     /locus_tag="PK839_01720"
     CDS             49043..50350
                     /gene="gldE"
                     /locus_tag="PK839_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated protein GldE"
                     /protein_id="HOG71483.1"
                     /translation="MDIAFHPITVGTVVGLLVLILLLIISGLMSGSESAYFSLSPADL
                     KKLESKSHQRAQSFVLINLNESERLLATILIVDNLVNVGIVILSTYLTNSLIDFSNAP
                     VAGFIFQVIVITFILLLFGEVMPKILATNRALKFALFMAYPLHLLVRLFRPLSSLLLS
                     STSRIGKRASTHNQISMDELSNALEITSDSIAEEKQILQGIVTFGSTIVSEIMKPRID
                     VVAVEYDTSFNHLKNVVVDSGYSRIPVYSETFDSIRGILYVKDLIPYIDREDDFHWQN
                     LIRTPYFVPESKRINDLLSEFQLKRIHMAIVVDEYGGTCGIITLEDILEEIVGEISDE
                     SDDETPLYTVVDSSTYIFEGKILLNDFCKVIGCSDDVFDDVRGEAETLAGLILEITGQ
                     FPSRNDVVTCKQFEFTVDMVDSRRIKRVKVKVHDREDEQSNSI"
     gene            50328..50906
                     /gene="gldD"
                     /locus_tag="PK839_01725"
     CDS             50328..50906
                     /gene="gldD"
                     /locus_tag="PK839_01725"
                     /inference="COORDINATES: protein motif:HMM:TIGR03512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldD"
                     /protein_id="HOG71484.1"
                     /translation="MSNRTPFSLLAILFLSVMLFGCADPPVPKPRGYFRIDFPAKSYR
                     LFDSIYPFRFMYPTYSVIVPKESADSGVWLNVDFTGYKATLHLSYKNVHGDISKMIED
                     ARSLAYKHTVKADAIDEQFFSNQQEKVYGVLYDIRGNSASSVQFFVTDSTQHYLRGAL
                     YFRCVPNKDSLAPAVDFFKKDVVRLLETLSWK"
     gene            50922..51533
                     /locus_tag="PK839_01730"
     CDS             50922..51533
                     /locus_tag="PK839_01730"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HOG71485.1"
                     /translation="MPIIKEILNEGNRIVVWQFHDGEFESPALQHESISDSVAHPKRI
                     LERQAVLAILKYMGFANGYSYTPESRPFIPGSNQHISISHSGSVAVVAVNKSTPVGVD
                     VEQLDRPFDRVAKKFLSKEEHLVVNVHDKSELALIWCVKEATYKLPWAKPCSLADDMV
                     VTLDRKQMESGWCRVKVRNYGSVAELKANFTFNGGYCLAWVNR"
     gene            51545..52258
                     /locus_tag="PK839_01735"
     CDS             51545..52258
                     /locus_tag="PK839_01735"
                     /inference="COORDINATES: protein motif:HMM:NF003198.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047294 - phosphoglycerol
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0006650 - glycerophospholipid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglyceryl/heptaprenylglyceryl
                     phosphate synthase"
                     /protein_id="HOG71486.1"
                     /translation="MTNIYSSLFSGGKSRQIALLLDPDKGNELSLSKTLELANQTRVS
                     MILVGGSLISGDINSFVLRVKEHTALPVILFPGSAFQFTSHADAILLLSLISGRNPEL
                     LIGSHVQVAMRIKQSGMEVIPTGYILVDGGAVTSVQYMSNTMPIPSDKPDIVVATALA
                     GQQLGLKLIYLEAGSGAKNSVPAELVRQVRSQISIPLIVGGGLRSARQVDAVFAAGAD
                     MVVVGNSVENNPAILEELG"
     gene            complement(52282..53937)
                     /locus_tag="PK839_01740"
     CDS             complement(52282..53937)
                     /locus_tag="PK839_01740"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HOG71487.1"
                     /translation="MHLKRLSLLILFTAGLRLSLWAQPNRQIPSEKPRLIVEIVASQM
                     RFDYLTRYWDKFSDGGFKMLVNDGASCKNARYSYLLTQAYPGIATISTGANPSIHGII
                     SDSWFSRVNGEVVDAVADEKTTTTGGSFASGRASAKNLVTSTLGDEICLNNPQSKVVS
                     IALDAGSAILMAGHSASGIYWLDTEKGGWVSSSYFSDSLPAWVDTLNAKSLAKLYAEK
                     EWKAINPIQAYEETDTLTARTPEGKKRVSDKIKLLIDGIIGKSEPATSYKKLLETPFG
                     NSLTKDMAIASILGESLGSDENTDLLCITFSANRYITRKFGPHSIEMEDTYLRLDREL
                     AHFLQFLATQVGKENVLVVLTSDQGVSSTPEFLEKSKIPGGYFDPKKAMVLLGSYLNA
                     LYGQGSWITGYHEKQIYLNRRLIEDSNINLAEMQQKVADFMLEFSGVANSATAHTLQS
                     ANFANGIMVKFQNSYNQRRSGDVIINLEPGWVERNGSITSGNSAYDYDTHVPLIWYGW
                     KVKRRVITSPVNIYDIAPTLSSLLGITWPNGSMGSPVRELVEP"
     gene            complement(54011..56833)
                     /locus_tag="PK839_01745"
     CDS             complement(54011..56833)
                     /locus_tag="PK839_01745"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HOG71488.1"
                     /translation="MKYLHITQLIVFTCLCTTLHAQHPERYFRFVEPSKETINTKITR
                     IISIDRVKADTVWAYANDEEFARFSDLGYKVELLTPPSLLPGKVINMATTIGQMASWD
                     RYPTYPVYRELMKKFEQDYPQLCKLDSIGTTVGGRKLYVLKISGNVQANEAEPEVLYS
                     STIHGDEVTGFVLMLRLADYLLSQYGSNSRITNLLNGTAIYINPNANPDGTYYSGDST
                     VYGARRYNQNNVDLNRNFPDPRDGDHPDGYSWQPETQAMMSFANEHHFILSANFHGGA
                     EVANYPWDTWTSAENTHADNSWYYTISRAYADSVHAHSPAGYFTDENNGVTNGGDWYV
                     ITGGRQDYMNYWHHCREITLEISSTKLLSSDELPAYWGYNRESLIGYLEHALTGFYGT
                     VTNPDGQPLDATITIPGHDRDSSHVVTNPEHGSYYRMIAPGTYEVLYQSDGYISQIHT
                     VSVPSYSSRIMKDVVLEPAQQVNLSGLATDSLTGNPIEGAKVELVGSSYTPVYTNAQG
                     IYVFSAIYEGYYQVRASKAGYIPSTQELNLTVSSNTLNFILAPSEAESFELQLPSTLT
                     FTGGSWSRTNQTAYTGSYSLRSASIGHSSSTAMTATQNFNGPGEVAFARKVSSEQGYD
                     FLRFYIDGTKKGEWSGEQSWGEESFSITSGTHTLKWEYKKDEMVIGGSDCAWVDDIIL
                     SPTLQNATFTVLVNGNEYEGLTVNLDGMTANTGSDGKVNFVNVPTGLAKPVSVQYMGN
                     TLADSLVNVVWTGQNSYDMDISAYFEVTFHVTSEGNPVEEATVTLNDLQLQTNSQGIA
                     TFTGIPFAPNIHVDISRSGYNTYEGDISIASDSTYTISLNPNASEPIIGNTQSLRIFP
                     NPFTDRVNIEIETSEYSQVQLEVYSILGCRMDNVFNGMLTPGIHNFTWKYDNMPKTQS
                     SGNIFMVWVRVNDTIIVRKIVQIQQK"
     gene            57199..57432
                     /locus_tag="PK839_01750"
     CDS             57199..57432
                     /locus_tag="PK839_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG71489.1"
                     /translation="MSTGKMYDKVFAIFGVFMVFFYIGLGVFIIFHPLLDHLDKALRL
                     IFGITLIIYGIYRIMYSYEKIKKVFFSKDDDDE"
     gene            57751..58713
                     /locus_tag="PK839_01755"
     CDS             57751..58713
                     /locus_tag="PK839_01755"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOG71490.1"
                     /translation="MGKISTFKQVVQLAILAVAIVAVLPACKNKGAKVPAETPTSGSI
                     KIEADESFKPIVDAEVSTFTSLYNLAHIQTVYKPERELIADFLNDSVQVIVSSWTPTE
                     EQRKTLLDAQTVVRTTLVAYDALAIVLNKGNNDSLLTYDNVKDIFTGKITDWNQLNTR
                     SKLGKISIIFDNIHSGNIRYFREKFNLPQQLPPNFYAVNSNAEVVNYVAKSPGALGIV
                     SVNWISDTDDSTSFDLGSKVKLAAISQQYFDKDSYYLPVQGSIYQKTYPFIREVNINS
                     RESFYGLGSGFISFVAGEKGQRIILKSGLVPATMPIRIVEVKNE"
     gene            58735..60924
                     /locus_tag="PK839_01760"
     CDS             58735..60924
                     /locus_tag="PK839_01760"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOG71491.1"
                     /translation="MRRINRALLPVALLSLTCGVGLQAQTLQEAITLTKNEQYDKAEA
                     MFQQLIKNEPGNSKVYFFYGENVLLDYFADTISNSLNVATKKAADIFNNGITAGPNEP
                     LNYVGLARVSFYNRDDAKAEEFRLKAKSLLPPYKKVSKIPNPKDYAFTLAKIGESYIR
                     FGQVDTSKAMPYIRQALQIDSRNSEVYIIAGDLYILVNDGSKAIKNYNLAQDWDPTSP
                     TANMKIGSIYVKGRNLMAAIPFYEQAIALDQNYAPAYRELGQLYSMAGRFNDSKKYFE
                     KYLELTKGNIPAKIRYVNALFYAKEYKEVIKNVEEIFAIDDSRAYLNRIAGYSAFEEG
                     NYELAKNYMDKLFASLDPERLIKKDYLYYARILSRKNQNLGKLTIEADNMEADLTKLQ
                     TKYASAKPGPAKDKAKIEIDALKAELDSVKTLVERSEAEITMALEAYDKAALFDGEED
                     LNITYEKGTVQYAAKRYADAAKTWMHLIDLGRNSENDYIQVGKAFYQAKEFDKADSVF
                     NAMVTKYPDNLQGYLWMANIASAKDPDSELGLAKPKFQLVLQKAAVDSVKYVKEMYDA
                     LRYLGYEALQNKRYDEAKAYYYRMMNLSPDYVIRAHSSLSTMYISMGDYANAIAENNK
                     ILAIDPNNEVAKSSIQYITQLQKSALPKASPNEISGVITDSAGEPIPGASVRVKDTAA
                     EAWTNAQGAYKFVMPEASEVLVVSAKGYKTVEIPVTKKRVYNVSLEK"
     gene            61030..61845
                     /locus_tag="PK839_01765"
     CDS             61030..61845
                     /locus_tag="PK839_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOG71492.1"
                     /translation="MSKNSKSSGGVLQSLMAPLTIILAFIVAYLIFTRVLGNPVNFEG
                     GNPKGHPLQGNLLGMMYKGGVIVPVLITVIITLLTYVIERYITLTRVKGKSRLNVFVS
                     NIQKMLAEDKIEEAIEACDKQKGSLANVMKAGLSRYRALENDKELEKDQKITSMKQAI
                     EEATALELPVLSRNMVFLSTIASISVLIGLIGTVLGMIRAFAALAQSGAPDALALSTG
                     ISEALVNTAFGITGSTLAIIFFNMYSTMIDNYVFKIDEAGFSLTQTFAASIRK"
     gene            61888..62526
                     /locus_tag="PK839_01770"
     CDS             61888..62526
                     /locus_tag="PK839_01770"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOG71493.1"
                     /translation="MPKIKLGAKSPRIDMTPMVDLFTLLLTFFILTATFRPQESAPVD
                     TPFSVSEKPIMDANVMTVLIAKDKRVFFNIDNGPDTILKFRPKVLAEMGKRYNIEFNE
                     AELRQFEKSGTAVGVPIQKMKEYLNTKNSDERRVLETGVPIDSTDNQLALWILFARQV
                     NPAVQTMIKGDADLEFPVVREILSILQDKNVNRYSLVTNLRAVEVKEDEIAE"
     gene            62528..63187
                     /locus_tag="PK839_01775"
     CDS             62528..63187
                     /locus_tag="PK839_01775"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOG71494.1"
                     /translation="MAEVQVQENTKGGKKKPKKGDAHVDMTPMVDLMSLLITFFMLTA
                     AFSKPKVMEIVLPEKIKKGEKVEPPKIAESRTLNVILGPNDKVYWYPGKADPEVTVLQ
                     ETDFSATGIRKVLLDRNRALFRKIDDFQKKVISGEIEISQDSLRSAISQLKKDDDTGP
                     IVLIKAYKTSKYKNFVDIIDEMSIVGIARYTVTDINWVEEKLVEKALGIAPVATTQTA
                     K"
     gene            63198..>63729
                     /locus_tag="PK839_01780"
     CDS             63198..>63729
                     /locus_tag="PK839_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG71495.1"
                     /translation="MTRTYYPSFDDIVFANRNKEYGAYVLRKKYTRTMALSIILGVLI
                     IVMAAIIPFIRAKSIAAAKARDANEVIAQMANDIQLDQAAPPPPPPPPPPTEQQTVVK
                     YVAPVVVDTVKPEEQSKLMIAEEAVETVVDKEVVEVVEVVEAPKEEVEYEAPAEVFVA
                     VEEMPSFPGGDAELFKF"
     assembly_gap    63730..63829
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <63830..64132
                     /locus_tag="PK839_01785"
     CDS             <63830..64132
                     /locus_tag="PK839_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005867932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HOG71496.1"
                     /translation="AVEEMPSFPGGDAELFKFIYENVKYPDIAMENNIQGKVIVRFCV
                     TYKGTVDHVSVLRGVDPTLDEEAIRVIKMLPKWKPGKQGGKPVNVWYTVPISFQLK"
     gene            64275..64577
                     /locus_tag="PK839_01790"
     CDS             64275..64577
                     /locus_tag="PK839_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG71497.1"
                     /translation="MAIRITELFGAKAGDDWGRHYMGIIKHCVLPNGQQGVFGRIDIK
                     DSEHNEVACASANSREQLGVKLDLLVLMVLDYGILDLKANGMNSDTFGTNMTIGLS"
     gene            65285..67534
                     /locus_tag="PK839_01795"
     CDS             65285..67534
                     /locus_tag="PK839_01795"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOG71498.1"
                     /translation="MRRILIVVAMILSYHILNAQNKITGKISDQANLPLIGVSVFVPD
                     MSKGTTSGTNGSYELSGLPNGKIKIQFSLLGYAGRIETVVLSGEGLELNIVLNQTALE
                     LEEIVVSGGYSSTQHENAVKIDVLKLDPRTGTTTPNFTETLTKVPGVDMISKGSGVSK
                     PVIRGLSMNDILVLNNGVRFENYQYSSHHPLGIDEFGVEDVEIIKGPASLLYGSDAIG
                     GVINFIKEKPAPIGSITGDYNMQLFSNTLGATNNFGIKGASNKFFGGLRVGQKTNSDF
                     LQGGGAFVPNSRFNEMSAKLNAGFTDKVGTFKLFYDFNNQKLGLVEDEAIEEISQRGR
                     KNEIFYQELNTHLLSSQNKLYLGKFKLDINSAFQRTELTHFGDAGVYEIQMKLATFTY
                     DAKLHLPSRVNSEYIIGLQGFNQTNTNLNNRETKLLPDATTSNYSAFGLLQYTFFRKL
                     KLQTGIRYDYKTIATKGIGVATDSMTYRAPLDKSYGSFSGSLGATYKVTEELLFRANI
                     AAAYRTPNIAELTSNGPHELRFEIGDRNLDPENSYETDVSLHYHKDNFIVDVAGFYNI
                     VNSYIFISPTGDTTASGIYIFSYKQANSILFGGEAGLHVHPKSLKWLHFETTFSSVIG
                     KQRNDLYLPFVPANKLRFELRGEKEKLALINNAFASISINTAFKQNNAAPDETPTAGY
                     TLIDLSIGGQIKVQNQLISFSISANNLTDKKYIDHLSTLKEVDLYNPGRNISLSIHVP
                     FELKASSEN"
     gene            67874..68089
                     /locus_tag="PK839_01800"
     CDS             67874..68089
                     /locus_tag="PK839_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG71499.1"
                     /translation="MVRLKGGNFNEIRKGETNELFVIVKNGLTEVAVIRINKENGCYI
                     VEKASVMRKAYVNRKELLWQRERTLLT"
BASE COUNT        18387 a        14730 c        15688 g        19338 t
ORIGIN      
        1 cagtgagcgg aagggttacc gatgccgaaa ctggcgaaac gctgcccgga gtaaatgtaa
       61 ttgtcaaggg tacaactcgt ggtacaacaa ccgatgtaaa tggaaattat aaaatcgagc
      121 tgattgcaga ggacaaaatt ttatcattct catttgtggg atatgaagat gttgagttgg
      181 aggtgcttaa ccaaaaggta gtggatgttg ctttaaaggt aaaatccact ttaatgcaag
      241 aggtggttgt tgttggctat ggaacgcaaa aagccaaaga tttaactgca cctattgtta
      301 ccgtgaaagg aaatgatttg tcaaaacagg ttacttcaaa tcctttaaat gcgcttcagg
      361 gaaaagtttc gggagttcaa attattaata gcggcgttcc cggtagcgga gcctcaatta
      421 aaatcagagg cgtggggtct gtgggtgatt atgctaatcc tctttatgtg gttgatggag
      481 tttttgttga caatattgat tttattagcc ctagcgatgt tgaagagcta actattttaa
      541 aagatgcctc tgccgctgca atttatggtg taagggcagc taatggggta attattatta
      601 ctacaagaaa aggaaaatcg gggttaccgg tagttagtta cgatggctat gtgggggtac
      661 aaatgcctgt taatattctt aaaatggcat caaaaaacca gtatataacc ctactaaacg
      721 aggcaaatgc aaacatacct ggatacatac ctaaaaatcc atccgactac ccaaccagca
      781 cggattggta taatgaactt gttagaacag ccggtataaa taaccacaac atagacataa
      841 caggtgctac cgataaaact tcttactcca ttgggggtaa ctttttgtac caaaatggaa
      901 taatggatgc tcagaataac tatcaacgtt acaatattag gggaaggtta gaacagcagg
      961 taaacaagaa tcttaaaata ggaatcaaca ctatcatatc gaattataat aaaaacattc
     1021 caaattcaga tgcctttttt ggagcctatg ttaacccacc ggtttacacg gtttatgatc
     1081 caactaatgc cgaagcttat cctgttaaat ttggagcccc ccagaaatat ggttttggaa
     1141 accagtatgg taaccctgtt gccgcagcat attatgctga taactttgaa aaggggcata
     1201 aggaggtttt cagcattttt ggagaagttg acattgtaaa ggacatttta tcctatagag
     1261 ttgcctacaa tcaagatcag gatagctgga atcaaaggta ttatacccca cagttttacg
     1321 ttggtggttc acagggcgtt actaaatcaa atttgacaaa aaccttcggc aactcctcta
     1381 agcaaataat tgataacctt ttaactctaa agaggacaaa gggtaaaacc tcatacactg
     1441 ttttgtttgg ccaatcaacc agaatagaaa agagggaaac tctttcaggg tatgccaaca
     1501 gcgttcctgg gtacgacgac cagtcgaaat acttagtaaa cggttcattc cgtgatcgta
     1561 acgcttacga tgctgctgaa aggtataatg gattgtcatt ttttacccgg ggaaccctaa
     1621 actttcgcga gaagtacctt gccactgtta ctttcagggc tgatgcaagc tcaaaatatc
     1681 aaaaaaaatg gggttacttt ccttcagttg gatttgcatg gaatatctcc caggaaggct
     1741 tttcaaaact taacgctgca ttttccaact taaaacttcg actaagctgg ggtatgcttg
     1801 gaaacgacaa tgtaccgtca aattcatctg taattcttgg gcaaaccgga attggcagct
     1861 ctggggtttt tggcgataac ctggttgacg gaatgggtgc ccagacggta cttcaaaact
     1921 atttgcgttg ggagacagtt actgagtttg acgttggcat tgattatacc ctcaaaaatg
     1981 aaaaactctc tggtgaagtt gactattacc atcgggttac taataatgtc gtattttatg
     2041 ctcccattgc taccggagga ggcgttgcag aattactggg taacaatggc aaggttctca
     2101 acacgggttt ggagtttact ttgaactgga aggatgagat atctgaagat ttcaactact
     2161 tcatctcttt taacgcaaca ttcaaccata acgaagtgtt ggaacttaac ggacgcgaat
     2221 atattccaag tggatatatt aggggaaact acaccacacg taccgcagta ggccatccta
     2281 ttggttcatt ttatggctat gaaatagatg gggtttacaa aagtgaggga gatgccctta
     2341 aggatcctgt gagtcaggct ataaaggata aagggtactt taagtacaaa gatcaaaatg
     2401 gcgacatggt aattgatgat aaggataagg tttacctcgg cagcgctata ccctggctta
     2461 tttcgggatt ggacatgggg ataaacttta agaaattcga cattagcctg tcaatcgctg
     2521 gccagtacgg gaataaaatt ctaaatgcta aacgaatgaa ccgggatgta ttctccgacg
     2581 gaaattatga tgaagatttc taccaaaaca ggtggacttc cagcaacaag tcgagcgagt
     2641 acccttctgc cgaagcctac aattcctctt ttattcagca agctaacgac tttttcgttg
     2701 aaaatggatt ctatatccga attcaaaaca tccagctagg atacacaact gataaaattt
     2761 cttttatccc caagttcagg gtatttatta ctgcccaacg accattcacc ttttttacat
     2821 ataagggatt tactcccgag gttggaggat ctccaattag cagtggcatt gataacaatg
     2881 tttatccaat gcaggccatt tacacgcttg gattaaaaat gatgttttaa atcattaaca
     2941 cattaccaaa atgaaaagaa ctcggcttac tttattgctt atagcagcct tactagccat
     3001 aagtagctgc gacgattttc tcgatattcg gcaggaggcg actttgccga caaccggtat
     3061 tgattacact aaacctgaaa atatttttct ttctgttagc gctgcctatt caaaacttag
     3121 aagttgggat gcccatacgt tcccatatat aggagcattt gaaattgcgt cggacaacgc
     3181 cgataagggt agcactcctg aggataaccc cgaaatgaaa gatattgaca atcttaactt
     3241 tcagccaaca aacggtttaa ttaacgggct atggacgggt tattttgata ttgttagcgg
     3301 tgcaaactat gctattcacc aaatgccttt gtttgttgaa accttaccta gccaaaccga
     3361 tagggattat gctatacagt gtcagggtga agcaaaagca attagagctt atgcatactt
     3421 caaccttacc cgtctttttg gaagggttcc aattgttgat accgttctgt catctgagca
     3481 gcttgcttcg ttgaagcaag ccagcactac tgagctgtac aattttatcg aaaacgacct
     3541 gcaggaagca attgctgttc tacccgatgg ctatactaaa gaatggggtg ggcgaatctc
     3601 aaagtacacc gcaatggcca ttaaagccaa agtgcacctt taccaggctg aatgggactc
     3661 agttgcttcc ctaaccgata aaattattgc ctccggaaga ttcaagcttc tagataattt
     3721 tcgtgaggtt ttcagccttg atggagaaaa ctctaaagaa tccctatttg aaattcaaag
     3781 ttcaactcta ggtaagtctt cgggtgatgc agcctatctc gactatggat atgtgcaggg
     3841 tccacgtggc aattcacctg ctaacatgca gggatggggg ttttgcactc cgagtcaaaa
     3901 tctgattgat ttttatgctg accgcggtga agtagtaaga ccagctacta cctttttgta
     3961 taggggcaca gttactccag aaggtgatag cattaaatca aactgtacaa acccggttta
     4021 taacgggaaa gtttatactc cctcatctca aaataggtgg gtttacaatg gttacggttt
     4081 cgatcataac attagggtca ttagatactc agatattctt cttatgtatg ccgaagccct
     4141 tatccagggt gctgctgttc cattgtcttc tggcttaagt gctgatgatg cagttaatct
     4201 agtacgcttt agagctggct tagacaacat tacaggagtt accttattgg atatatggaa
     4261 cgagcgccga gcagagctcg ccttggagga agacagatac tttgatcttg ttagaactaa
     4321 ccaggcagca actgtccttg cctcaaaggg atttgtggtg gggaaacatg aacattaccc
     4381 cattcctgct gcacaaatgc agcttaatcc taatttaacc caaaatacag gatattaacc
     4441 agttatctta attcaaacca atgtttaact taatccaagt gctatgaaaa acaaattcag
     4501 aatttttggt acgcttttac ttggcgtaat ggttgccgga actctaactt ttagttcctg
     4561 cagtagcgac gatgatgatg acaataacaa caatggcaag gttgatcctg gtaccattgc
     4621 tgaggcaaac ctggtagctt atttcccttt tgatggaaac gctaccgaga aaattgcaaa
     4681 tctaacacct actcaacaac ctggagtaac ctatgttgct ggacggagag gtcaagcata
     4741 ccagggagct gaccaggctc atttacttta cactattccc gatggaagta aactgaaaac
     4801 gttaacttcg ttttctcttg ccatgtggtt aaaatcaccc ctggtaactg gagatcctga
     4861 accaactatt ttcgaaattg gtaaatctga tgacctgttt tggggtaatt tgaaatttgc
     4921 tttaaacagg cttagcgcta ctgctgattc tctccagtta aaaacatttt ttctgaaatc
     4981 agacgctaca acatagaagg gtcaacacat cagctattca aatccgtctt ttcagattaa
     5041 caagtggatg cacattgttg tgcagtacga cggaaccact tctaaattta tgatttacaa
     5101 agatggcgtt aaggtgaaca ttcctgcaaa catcgaaaac agaattgatg gtgaaaatgg
     5161 acctgctctt ggtcctttag cctttgaaaa tgttgataag cttaattttg gtgcttggcg
     5221 tactaaatct gagggtggct taagtgatgc atggatggga tggtaccttg gaaatatcga
     5281 tgaatttcgc gtttacgata aagctttaac tgcaactgaa gttaaagccc tatatgacgc
     5341 agaagtttct cagattaccg agtaatctat acttttaaaa aaagggtaag gggtgatacc
     5401 cttccctttt ttcccttttt atttcaatta ttgttgtgct caattttatt ggcttaaaaa
     5461 atttcctgct ttgataaatt tttctaaatg gtagttagta tctaccgtca actttcttaa
     5521 aaatgaaatt tcaccttttc tctattttat tttttctact agtgattttt tgccaatgca
     5581 aaaaggatga tgctataatt agaattggca atgtaaatgt tgattctgtg ctaatagatg
     5641 gtattaaagt agctgacaat ggcagtttat ataatgtatg tttccaaccc aaaattcaag
     5701 tttttttcag caacacggtt gatagtacaa agcttaacac taatgatatt tatttttcta
     5761 gcaatctttt cgataactac tcctataagt tctcaagtaa tgtaaaatct cttattcttt
     5821 ggtcaaatga aaccttacca cccctaacag tttttcggtt taccattatt caaggaaaaa
     5881 atttgggtgg caatattatt aacgggttta aagccaagtt ttataccagg gtcgattcat
     5941 ccgagaagtt tccttttatt cccgacgatg acctgcttac gcttatccag caacaaactt
     6001 tccgctactt ctgggatttt ggccacccat catgtggaat ggctagggaa agggataact
     6061 caggcgatat tgttaccatt ggtggtagcg gatttggact tatggccatt cctgttgcca
     6121 tcgaaagagg ttttattacc cgttccgaag gtatagagcg aattgccact atggtcaatt
     6181 tcttaaaaaa tgccgacagg tttcatggcg catgggcaca ctggtacaac ggtgctacag
     6241 gacatgttgt tccttttagc acctatgata atggtggtga cctggtggaa acatcctata
     6301 tggcacaggc gctgctaaca gtcaggcagt atcttagcgc tgccgaccct gctgaagctg
     6361 agcttatcaa caatataaac ctgctgctcg atggaataga gtggagctgg tacaaccgcg
     6421 ataacctgaa catgctctcc tggcactggt cgcccaacta tgggtgggct atgaacatgt
     6481 ttgtgagggg atggaacgag gcgctgataa cctacatcat ggccgctacc tctagtacat
     6541 ttcccatatc tgcagaggtt taccataatg gctgggctgg gaacggggct atagttaacg
     6601 gtaggtcgtt ttatggatac accctgccgc tgggtcatga ctatggcggg ccgctcttct
     6661 ttgcccagta ctcttttctg gggctgaatc cgcaaaactt aagcgaccag tacgccagct
     6721 actggactca gaacgttaac catacgctca taaaccgtca gtactgcatt acaaacccta
     6781 gaggttacct gctctacagc cctgattgct ggggcttaac cgcctcggac gacaacaacg
     6841 gctatggcgc tcacgaacca ttgaatgata acggaaccat cacccctacc gctgcactgt
     6901 cgtcaatgcc ctatactccc gaggaatcca tgagggtggc tagatttcta tactacactt
     6961 taggcgacaa gctatgggga gactacggtt tttacgatgc ttttaacccc actgccgggt
     7021 ggtggggaaa gtcgtacctg gctatagatc agggaccgat catcatcatg attgaaaatt
     7081 accgaaccgg gctgtgctgg aacctgttta tgtctgcccc ggaggtggat ggtgcactca
     7141 gcaagcttgg tttttcacac taaaattgtt taaagatcat gcgtattagg aagctatctg
     7201 aaattacact tgctgccatt atgagctata ccatactttc atgcagtaag acaaccatgc
     7261 catactccga tgtggttgac cagaaggttg actctattct gatgctgatg actcttgagg
     7321 aaaaaatcgg acagctaaac ctggttacca gcgatatgga tgttactggt ccattcattc
     7381 gtaaggggta tgaggaggat attgcaaagg gtagagttgg ggccatattt aatgcctatg
     7441 gcgcaggcta cacccgtaaa cttcaggagc tggctgttaa taataccagg ctccgcattc
     7501 cgttactctt tggctacgat gtgattcatg gccacaaaac catattccca gttcccatag
     7561 gcgaggcctc cagctgggat ttagaggcca ttgagcgttc ggctcgggta gctgccgagg
     7621 aggcttcggc tcaaggccta cactggactt ttgcccccat gtgcgacatt gcccgcgacc
     7681 cacgatgggg gagggttgtt gagggttcgg gcgaggatcc cctgcttgga tcactcatag
     7741 ctcaggcaag ggttagaggt tttcagggaa ccgacttaag tcaaaccaac actatacttg
     7801 cttgcgtgaa gcattttgct gcttatggtg cagctcaggc tggccgcgac taccatagcg
     7861 ttgatatgag cgaacgacag ctgcgtgaag tttacctacc gccatataag gctgctattg
     7921 atgccggcgc actttcggtt atgtcctcat tcaacgacct taatggcatc cccgccacag
     7981 ccagtaaata ccttatgact gatatcctcc gaaatgagtg gggttttcag ggatttgtgg
     8041 taaccgacta cacctcaatt atggagctgc tccaccatgg agttgcggcc gataccctcg
     8101 atgcagcccg cctggctctc aatgccggaa ttgatatgga tatgcagtcg ggattctaca
     8161 acagcgcctt gttgaagctt ttaaatgaaa agaaaatcaa cgaggagcaa atcaaccagg
     8221 cagttagaag gattctacga atcaaattct tacttggttt gtttgacgac ccctaccggt
     8281 actgctctat ctccagggaa aaggagaccc ttttaagtaa tgataacctg ttggcagcta
     8341 gggatatggc taaaaaatcc attgtgctgc tgaaaaacca aaataatctg ttgccactcg
     8401 acaagaatat taaaaccata gcggtggttg gtccccttgc taacagccaa agggatatga
     8461 tgggcagctg gtcggctgca ggtgactggc caaattcggt tacccttctt gagggcatta
     8521 agaacaagat accaggttca caaattctat acgcacaagg ctgtgctata gacgattcca
     8581 acaccggtat gattgatgag gccgttaaaa ttgctaaacg tgccgatgtt gttatactgg
     8641 cagtgggaga ggctgcatgg atgaccggtg aggcctccag ccgcgccagt cttgaccttc
     8701 caggtgttca gcaggaactg gttgaaagga tttacagcac cggcaagcct attgtggctg
     8761 tgctgatgaa cggaagaccc ctcacaatcg gctggataga ccagcacata ccctcaattc
     8821 tggaaacatg gtttcttgga actcaggccg gtaacgccat cgccgatgtc atatttggcg
     8881 actacaaccc ttctggtaag cttcccatta ccttcccgcg ttcggttgga caaatcccca
     8941 tttactactc catgcgaagc accggtcgcc ctttcgatgc aaacaataag tacacctcaa
     9001 agtacctcga cgaaagcaac gatccgctct acccttttgg ctacggatta agctacacaa
     9061 ccttcgatta cggggaggtt gagttaagct ccagtgaaat taccgagacc gatactcttg
     9121 acatatccgt aagtgttacg aataccggga agtactatgg tcgagagatg gtgcagctct
     9181 acattcatga taatgtagcc tcggttgccc cacccgtaaa gcttcttaag caatttcaat
     9241 ttgttgacct taaacccggt gagagtaaaa aaatggtatt caagcttacc gtaaacgact
     9301 taatgttcta ccgggatgat atgtcctacg gatttgaaaa gggacaattc actgtttata
     9361 ttggtggtaa ttcgcgtgat gtaaggacag cgtttttttc tttaaattag agtttgtttt
     9421 aggtttggtt tggagaatag gtggaagtat attccaccta atcttttatt agtgaatgga
     9481 gtaagtgggg tgagctattt tactacattt tttgagatgg attagttcgt ggtgaatgca
     9541 gctgttgatt gagtatttca ccatttatcc aggcactcta cgaagttaag ctcccacttg
     9601 tcaaggtcaa gggcaaccag gtagccggta ccggggaaaa gcatccccct gtacacacaa
     9661 ccggcatcca acccaaaaat tcgcctgttg ggctgcttga gtttgtcggc tatcacatct
     9721 agcgcttcgg gggtatgccc atgtattatc ctgtaatcct gcataaaatc gtttggggca
     9781 tcgtcaagcc tgctccagag catggcttct gtatcctcca ggggattggc tgcggtagtg
     9841 ttcagcccgc catgtacaag aatgtactta tcgtcaacga tgaagtattg ctttagcgag
     9901 gtgaaaaagt ccaaatgtgc tttaggaaga aagcccaaat gaccatcatc atcaaaacca
     9961 aacattttaa cgtagctttt aatggtggct tgacccgagt tggcctgcca aaggcatagc
    10021 tccgactctc cctttaccga gtcaagcatc atctgctcgt ggtttccaat aaggcagctg
    10081 atggtgtagc ccgatctcat taacccaatg atgtactcca ctactgccga gctatcgggg
    10141 cccctatcaa tgtaatcgcc caggaagaaa agcctgtcct gtagcgatac ctttagcttt
    10201 ccttcaataa gcatcctaag ggtttgtatg caaccgtgaa tatcggaaat gatgtaggtg
    10261 gccatggaag taatattcta ttcaaatgta cacgacattt ttttaacatg ctgatgaatt
    10321 tttaaattat tgaatgattt tttggtctga agcagataag gcttattttt aactagtttt
    10381 taccagcttc aaccatgttt tcagagtgat attttcattt ttttaggatt tagaaaactt
    10441 tttagtcggg tgctatttag aatcgtttta aattgtagcg ctgtaaaaca aaaatggata
    10501 atggtatata aagaagttta tttttgtagc tgttattatt tatttagaaa tgccttcagg
    10561 aaataatgta gttgagcatc tgggacgtgt tgatgccata acaaataacg acatccgggt
    10621 tgttataacc agccagtctg catgcgagtc gtgccatgcc cggggcgcat gtctggcttc
    10681 ggaaagcaag gaaaagttta ttgtagttac tatacccaac cataatttta ttgtaggcga
    10741 acctgttaag gttctactta aggaatcctt aggatttcgt gccctttttt tgggttatgt
    10801 tcttccattg cttgttgttc tcacaaccct tttcacgctg agcgcagccg gtatgtccga
    10861 aaaggacgcc ggtttacttt cgctggcagt cttggctccc tactaccttg ggctttatat
    10921 ttttagaaag aagattgcaa ggaagtttga atttggaatt gaaaaaattt cataataaaa
    10981 acatcgtatg agttcagttc ttactacgtc tattttaatg ctcacgattt tgggggttgt
    11041 tttagccgta atcctttatg ttgttgcgca aaagtttaag gtagaagaag atcccaggat
    11101 tgatgaggtt gaagccctgc tgccaggtgc caactgcggc ggatgtggtt atcccgggtg
    11161 cagggggttg gccgatgcgc ttgttaaggc agacgatatc agcgcgatct tttgccctgt
    11221 tggcggtaac gaaaccatga gaaaggtggc tgccatactt ggtaaagcag ttgaggacaa
    11281 ggatccgcaa attgccgtag tgcgttgttc gggaagcccc caaaacagga cgcgagttgt
    11341 agagtataac ggaatatctt cttgcaaatt tgcctccaca ctttacagcg gcgatacggg
    11401 ttgccaattc ggatgcttag gtcttggcga ctgtgttgcg gtttgcaagt ttgatgccat
    11461 ctatattgac cccgtttccc ttcttccggt tatagacgag gagaagtgca ctgcttgtgg
    11521 ggcatgcgta aaggcatgtc caaaattact tatagagctg cggaaaaagg ggccaaaggg
    11581 caagcgaatt ttcgtgtcgt gcatgaatac cgacaagggt ggtgttgcac gtaagtactg
    11641 cagcgttgca tgcattgggt gtttcaagtg cgtgaaggtt tgcccttacg aggccatcac
    11701 cctggagaat aacctggcct acattgatgc caacaagtgc aagctttgca ggaagtgcgt
    11761 ggaagaatgc ccaaccgatt ccattttgga ggtaaacttc cctccacgta agccgaaggt
    11821 tgaggaacaa gcccagaacg cagtaaccag ctagcataag ccatatcaaa cttaatttta
    11881 aaaaaaattg ctaaacgtgt tgaaaacatt taaaaagggg ggagttcatc cacctgaaaa
    11941 taagatttca gcagctcaac cggttgaggt tttgcctctt ccggctaaag tcatcattcc
    12001 tctgtcgcag catataggtg ccccggcaga ggcgatggta gccaaaggcg atagggtaaa
    12061 aaccggacaa ctcatagcca aaagcgctgg ctttgtgtcg gccaatatcc attcaactgt
    12121 ttcaggaata gtagaatccc ttgaacccgt gatggatagt tcaggctata aacggcctgc
    12181 cataaccatc aacgttgagg gagatgattg ggagccttca gttgaccgtt ctcccaactt
    12241 aaagaaggaa ataacccttt cggctcagga tattataaaa aaaatagcgg aggccggtat
    12301 tgtgggcctt ggcggtgcaa catttccaac ccatgtaaag ctttccgttc ccgcaggcaa
    12361 gaaggctgaa atgctggtga ttaatggtgt tgagtgtgaa ccctttctta cctctgatca
    12421 taggctgatg ctcgagaaag gccacgagct tatgattggc attcagattt tgatgaaagc
    12481 tctgaatgtg cctaaggcta tggttggtat tgaggcgaat aagcccgatg ctgtatccca
    12541 tctccaatca ctggccaagt catacagcgg aatagaggtt caacctctaa aggtgaagta
    12601 ccctcagggt ggcgaaaaac agcttattaa ggcgcttacc ggccgtgagg ttccttcggg
    12661 tggattgcca attgatatag gtgcagttgt tcataacgtg ggcacagccc ttgcagttta
    12721 cgaggcggtg cagaaaaata agccgctggt tgagcgcatt gttaccgtta cgggtaaaaa
    12781 tcttgcccat ccatcaaact ttttggtaag aattggaacg ccagtttcgg cactgatcgc
    12841 cgctgcaggt ggcctaccag atgatacagg taaggtggtg agcggtggcc ccatgatggg
    12901 aaaggcagtt agctccattg atgtgcccgt tacgaaagga acttcgggaa tattggtaat
    12961 tcctgctgcc gaatcaaaac gtaaacccga aggtgtttgc attaggtgtg ccaagtgcgt
    13021 ttctgtttgc cctatggggt tggagcccta cttgctttac cggctggggc agcgaatgca
    13081 gtatagccaa atggaggtag agcgtgtgct cgattgcatg gagtgcggtt cttgcagctt
    13141 tgagtgtccg gctaacttgt ttttgctcga ctttatcagg ctaggcaagg ttgaggtagg
    13201 caaaattgtt agggagcgga aaaattaaca tttaaagtat atactgacag taacctttta
    13261 aacgaatgga taatcgtttt gtagtttcac catcgcccca tgtgcatagc ggcaaatcga
    13321 ccagccagct gatgctgaat gtggttttag cgctgatgcc ggcgtttgcc gtttccattt
    13381 actactttgg cgtgggagcc ataattgtta ctctaatatc ggttctttcg tgcgtgctat
    13441 tcgagtacct gattcagaag tttattttca aggttaagcc gacaatatgc gattgctctg
    13501 cggttgtaac gggtttgctg ctggcattta acgttccgag caatctgccg tggtacatta
    13561 tagtgctggg aagcctcttt gcaattggcg ttgccaagat gagcttcgga gggctgggca
    13621 ataatatttt caacccggcc atagccgcac gaattttcct gctgatatcc tttccggtgc
    13681 aaatgaccag ctggccgcta ccggttacct ctcgtttaca gtatattgat gctattaccg
    13741 gtccaacccc tttggctatt attaaggagg gggttaagaa tggccaaacc atttcgcaaa
    13801 tggccgacag cattccttcc cacatgcagc ttttcatggg cttgatgggt ggctcgttgg
    13861 gggagattgc tgctttcgcc ttgcttattg ggtttgcatg gcttctcatt acccgcgtaa
    13921 ttacctggca tattccggta tcaattttta ctaccatttt cgtattcaca ggaattttat
    13981 ggcttatcaa ccccggtatc tatgccgacc cggttttcca cctccttacc ggtggtgtga
    14041 tgctgggggc tatattcatg gccaccgact atgttacctc acccatgcat cccaaaggca
    14101 tgtggatata cggaataggt attggcgcgc ttactgtgat aataagggtt tggggctcgt
    14161 accctgaggg agtttcgttt gccattctga ttatgaacgc tttcgtaccc cttatcaacc
    14221 gattcgtaaa gccaaaacgt tttggtgcag cagcaataag cggtaaatag catgtaaaac
    14281 ttaaacatca aaatatgtcc ggtgtagaat catcatttaa gaatatgacc atttccctgc
    14341 tggcgattac ccttgttgca tctgccggat tggggctggt ttataaatca accaaggatc
    14401 ctattgcagc cgctcaggtg gctaaggtga acaacgctat taaggcggtt gttcccgaat
    14461 ttgacaacca accatccaac gaagcataca ctgtaaaggc cgatggtgat gatttgtttt
    14521 tctatcccgc ctccaagaat ggccagcttg tgggcgtagc ggttaaaaca ttcagcaaca
    14581 atggctttgg tggaagaatt gagctgatgg tcggcttttt acccgatgga accatcaaca
    14641 agattactgt aatttctcac aaggaaactc ccggtttggg tgataagata gacccaggaa
    14701 agtccgattt ttccgttcaa ttcgagggta aaaatccgga aactttcaag gttatggtga
    14761 agaaggatgg gggcgacgtt gatgccataa ctgcttcaac catctcgtcc agagcctttt
    14821 gcgatgcagt taaccgcgcc tacaaagctt ttaaggaagg gggtagaaaa tgagcgcatg
    14881 gagcaacctt acgaggggta ttatcaggga aaacccaacc tttgtaatag tgctggggat
    14941 gtgtcccacc ctggccacca ccacttctgc tattaacggg ctaggcatgg ggctggccac
    15001 cacctttgtg ctgttgggat caaatgttgt aatctccatg attgcacgtt ttatccccga
    15061 caaggttaga attccctcct acattgtggt aattgccaca tttgttacta tagttgacct
    15121 gcttatgcaa gcctacacgc agtcgctcta tgaaaccctg ggaattttta tacctctaat
    15181 tgtggttaac tgcattgttc tgggtcgtgc tgaggcattt gcccgaaaga atagcgtgct
    15241 cgattcagcg atggatgggt tgggaatggg aattggattc accatggccc ttacgctgtt
    15301 gggctcaatt agggaaatgc tgggaaacgg atcgctgttc aactacaagt ttattccggg
    15361 tgatgccatt ctggttttca tcctttcgcc gggtgcattt attgccctgg catacctgat
    15421 tgccctattc aacaagtttg ttaaaaagta ggaggctgtt atggaatacc tgataattat
    15481 tattgctgca atatttgtca ataacgttgt actgtcgcaa tttttaggaa tttgcccatt
    15541 cctgggggtt tcatcaaaaa tcaacacgtc agttggaatg gggggagccg tggtgtttgt
    15601 catggtgctg tcaaccattg taacctatct ccttcagcaa tacgtgttaa accccttgaa
    15661 catagggtat atgcaaacca ttgttttcat tctggttatt gccgccctgg ttcaaatggt
    15721 ggaaataatt ctaaaaaaga ttagtccttc cctataccag gcgctgggaa ttttcctacc
    15781 gctcatttca accaactgcg cagtgctggg aacagcaata ctggtcataa agcataacta
    15841 taacctgctc gaaggagttg tttttacggc tgcaaccgct gtcggttttg gttttgccct
    15901 ggtaattatg gccggcattc gcgagcagat ggagcttgcc aatatcccta agggtatgaa
    15961 gggaattccc attgcgctgc ttacagcagg aattctttcg cttgcattca tgggatttgc
    16021 cggaatagtc aagtagggtg gcacaagctg cattccctcg aacccaattt ggtttaatgt
    16081 taaaggtaaa agcttggcat tgagtgtcat ttaacttctg ttgcccttac cattattcct
    16141 aatggcgctg tgtgcttcct accagcctat acctggcaaa accaacctgc aattaagcgg
    16201 tacacttttc taacatggca ggcaatccgt tttaaacctt ttttaacagt tcaaatcata
    16261 ttaaaacgtg catcatggac taccttagca gattaagtag ggggtaacca tgagaacggg
    16321 ttttatcggg cagcttctga attacttacg atttagagtt aactgcaaac ggcggggggg
    16381 ctatgggata cactctcctt atgtttacag cctctatacc gaaacactta atgccgattc
    16441 taaaaacaac aaggtctttg agagaataga gcagtaccga aaggatttaa ggagatgtgg
    16501 gttaagcatt gtacgccaga ggcttggcga taatcccgaa aatggattcc ccggcgataa
    16561 ggttaagctt gccagaattg ccaggaacgt ttcggttcca ccccatttgg gacggttgct
    16621 tttcaggtta tgcagtaggt ttaaccccga aatcatagtg gagctgggaa catctgtggg
    16681 cattagcacg atgtacatgg ctgcagctaa ccccaattct aaggtttaca ccatagaggg
    16741 ggaggagtct atccttgcaa tagcccggaa tagattcaag cagatgggtt ttaccaacat
    16801 cgaacccatt gccggcaact ttgatgttca gctacccgag ctcataaaac gaattagcac
    16861 tattgatttt gcctttattg atggcgatca caatggcaga gcgctcctcc aaaatttcaa
    16921 ccggatgtta ccaagggtag ctgacacttc tgtttttgtg attgatgata ttcgatggag
    16981 ccccggcatg gaagctgcat ggaagcagat atgcagcaac ccaaaggtta ccatttctat
    17041 cgaccttttc cgatgcggaa tactgttctt taggactgga attgcaaagc aacatttcct
    17101 gctacgttac ggcccatact aaattttaaa ctttgcaccc taaatattta ttatacattt
    17161 gtagaacaac ataacggaac aaaattcaat gagcagcacc aacttaatca cgctgaatga
    17221 aataaaaaaa ttctaccagg ttggtagtgt tacggtgaag gcgctaaagg gcataaattt
    17281 aacaattgac caaaacgagt atgtggccat tatggggcct tcggggtctg gaaaatcaac
    17341 cctgatgaac atcctcgggt gtcttgatac cccaaccgat gggacctaca tactaaatgg
    17401 aaccgacgta agccgcctta gcgacgacga gcttgccgat attcgcaaca aggagatcgg
    17461 ttttgttttt cagaccttca acctgttgcc ccgctacacc tctctcgata atgttattct
    17521 accccttatt tacgctggag tgccaaagca cgaacgtgtt gagaaggcta agcaggtgct
    17581 ttacaacgtt gggttggggg atagaatgga gcataagccc aacgagttat ctggtgggca
    17641 gcgtcagcgg gtggcagttg cccgggcgct tgttaacaac ccgtccatca ttctggccga
    17701 tgaacctacc ggcaacctcg actccaaaac ctccatagac atcatgaacc tctttgagga
    17761 tatctactcg cagggtaata ccataattat tgttacacat gaggaggaca ttgcccgtca
    17821 tgcccgccgg attgtgagaa ttcttgacgg aatgattgag tcagatgagg tcaaccccca
    17881 tccaaccctg aggcaaaagg ttgtccttac agagcagtag cacaacattc aacactccat
    17941 ggtatagcta aattccttgg acagtccttt aatgctgaac tcctttcatc aaccgatgaa
    18001 aggagttcag cattcttgag ccttaaagga tcgagtttga cttcaacgta acttccctac
    18061 ttaagccacc tgtcaagcca gcttctaaat tccctctgcc agagtattcc attttgaggt
    18121 tttagcaccc agtggttctc atcggggaaa aatatgaact tgcttggaac gccaagtatt
    18181 tgcgcagcgt tgaatgcagc cattccttgt gtgtagggaa ttctgaagtc gttaccgccg
    18241 ctgatgatta aaattggcgt atcccagctt gttacaaagt tatgggggct ttgtgcgtag
    18301 ctcttcatgg ctacgggatt gttcttttcc catggtgctc cgcccatttc ccatgtgtca
    18361 aagaacatct cctcggtggt gaggtactcc atttcggaat gaaaaacacc gcagtgggca
    18421 ataaacgctt tgaagcgctt gttgtggttg cctgccagcc agaaaacaga gtatccacca
    18481 tagctggccc ctactgcacc caggcggttt tcgtcaaccc aaggctcttt ctttacctcg
    18541 tcaattgccg ataaaaggtc tttcatatcc tgaccaccgt gattcttgct gatttcatcg
    18601 gtccatttct gtccaaaggt aagcgtgccc cttcggcttg gtgccacaac cacgtatcca
    18661 cccgaggcca ttatggaaaa gttccagcgg tagctccaga attggcttac tgcgctttgt
    18721 ggtccgcctt cgcagtagag gagtgctgga taactttttg tgctgtcgaa atcgggagga
    18781 agaattatcc aagtgtgcac gagctttcca tcggttgact ttatccatct ttgcctaacg
    18841 gtgggcattt ttagctggga aagcagctcc tggttggtaa aggagagctc cacctcttct
    18901 ccattttgag ggttaatgga gtatatttcg acaggtttta cgatggaagc cttgctccct
    18961 atcaacctat caccggcaag ggcaaaagca tgatagtcgt gccacccatg ggtaattgct
    19021 acaggtttag cgtcctgcaa ggctaacctg taaatctgat aggttgcctc aactcccgct
    19081 ataaagtaaa ccatatctga gctggcattc cagattacgt ttgaaacaga gtagtcaaaa
    19141 tcacgggtta ggtaggaagt tttttttgtt tgaaggtcca ttacaagaag tcgatcctta
    19201 tcggcctcaa aaccggctcg ttccatggac aaccatgcga ttttttcccc atcgggcgaa
    19261 aatacgggtt gcttatcgta acccggcaat ccttcggtta agttttgggt ctttccattt
    19321 cttagatcgt agaggtagat gtcggagtta gtgctgaatg cggcctcttt tcctgtaagt
    19381 tttttacatg tgtaggcaat ttgattacca tcggggctcc aggcaatatc ctccatacca
    19441 tcaaatggtt ttagaggggt atcatagggc tcattcgcca taatgtcgat gatttttgat
    19501 aaggattttc catcgaagtt ggccaccatg acatggttaa acaggctgtc ctcccaggtg
    19561 tcccagtggc gatacattag cgtggtgtag atttgcccgc ttgatttggg caggtcgggg
    19621 taaatatcgg caattgaagc tcccaccttt acatcacttg taaggagaat ttttgaacca
    19681 gtaggggaga tgctgaatcc gcttatgtcg ttttcaaatc cttcaaccat ggaggttttc
    19741 tggttttcaa cattcatctg ccagatttgc gatttaccac tttcggttgc gaggtaggcc
    19801 aggttaaggc tatccaccca ttgcaggtta aactcagaac cgtttgtatg ggtaagctgg
    19861 cgctttccgg tgccgtcggt gttcatgatg tagatctccg agttacccct attttgatca
    19921 atagagtacc aggcggttgt gtatgcaact ttctgtccat cgggcgacac ggcaaattcg
    19981 cctattcggc caaacgacca aagaatctct ggagtaaggg tgctatcggt tactacaggt
    20041 ttaacaggcc caactatagc ttcattattt ggttgctggg ttttgcatga ggccagtacg
    20101 agcgctggca acgctaaagc cattagaatc tttttagttt gcattgctta gctattttgg
    20161 tatagtttac taggtagaat tagctttctc acaaggttat acaccgattt aaagtcgaat
    20221 acccagaatg gttatatcgt cgcgctgggg ttcgctacct gtaaattcat taaactcctg
    20281 tagcaagacg gatgaaattt cttccatggg tttctcaata ttggcgagaa ggtaggtttg
    20341 aaaccgtgcg gtggtaaact tgcgttttga ggagttattc tgatcggtta gcccgtcggt
    20401 gtacatgata atcatgtctc ctttgtgcat gggaactctc cagtttgtaa attccagatc
    20461 cacatcaggg agcaggccgc ctatggactt tctattaccc ttaagggttt gtattctgtt
    20521 ttcagccttg ctatagcaga aaattggcct gttagcgcca gcaaaagtaa cggaaaacat
    20581 atcgtttggc ttgctttcaa ttaagcaaag ggctatatcc atgccgtcga agctctcgct
    20641 gacattttgg tggagtgcca ccttgatgga agcgcttagc tcggtgagga tatctgcagg
    20701 gttgtagatt tttttctcgt ttacaatttc gctcagcagc ctgctgccaa ttaacgacat
    20761 gaatgctccc ggaaccccat gcccggtgca gtccaccaca gcaataaaaa ttttttcgtt
    20821 tgggttgctt ttagggccag catgggatat ccagtaaaaa tcgcccgaga ccagctcctt
    20881 aggcttataa attaggaaat gctcaaaaat atttcccagc gattgggata aatcgggaag
    20941 aatggcctgc tgaatggtct gggcatatat aatgctatcg ttgatgtggc ggttgtgttc
    21001 ctccagctca tcccgctggg taaggatttc ctccttttgc cgttgtagct cgacgttctt
    21061 ttcaataagg gcctgttcgg atttcgagag ctctaaggtt ctttcgtcaa ccagctgctg
    21121 gagtttaatg tttgagtttc taagcagctg ggtattaatt ctgatggata gccagataat
    21181 tagcaaaagt gcaactacat agccagtaat tgcccaccac tggaggtaga gaggtttttg
    21241 aatggtgaat tggaatgttt cggcaggact ttcaatgtta aagaggtttt ttgccttaac
    21301 gttgaactgg tatgtgccaa agggcagatt ggtgtactcc tttctctggt tgggttgcca
    21361 atcactccat gttttatcaa atcctaccag catgtaggag tataagagtt tttgctgctg
    21421 accgaagaac ggagcagagt aggtgaatgt taaggagttg tttctgtaag gaagtgttgt
    21481 ggattttact tcgttgtagg ggaggcttgt aggaaatgtt atcccctcat tcccatcgta
    21541 gtaaaccccg ttaaatagta tgtggtcagg accggccgaa acctgcctaa tgagaaccct
    21601 aaatatcgga ttttttaaat ttccggcgtc ctcatcgaaa cggaaaagcc catcggtaca
    21661 agctatccaa acaaccttat tattatcttc gggaaaaata tcgtagaaaa tagtttcggg
    21721 gatggttctg aattgggcag gttccctgcg gtaaatattc tttggatatt taacagcgcg
    21781 ctcaagccat cggtttgcag aaaaattgta tgcctcaaac caaatgttac cgttttggtc
    21841 ctggttaagt attcgtaacc cggttgttcc attggcaaat tccaacccta gggaggagtc
    21901 gggttcaaac atgttactct ggtggctgaa ttttgaaagt ccacgctcgg cagaagcttt
    21961 tacttctcca tcaatcataa ccacagcaat atcgtcgtac cttggattga atgggagggt
    22021 gtaaagaata ggtttttccg agaccagctg gtaggggtta accatatcaa cgtagttgat
    22081 acccttgtac cgttcagagt accataggtc tccgtttaaa tcttctgcca gggagtaaac
    22141 ctccccctta gttccggtca gctcccccaa aaatttgaac cgtccgttgt tttctgctat
    22201 tacaccgaca ccttcgcgta acccaacgta ggttattttc gggtacagct tcgattggag
    22261 aaggcagtag gaatggtact tcccattttc aattagtgtc gcctttcctc cttctacctg
    22321 aaaaattcca cttgaggtgg tggcaagcaa ggcctcatcg ccattcattg tgtgtatggt
    22381 tcgcagcttc catgataaat cggataaacc cgtaacctgc tctatcctgc cgttgctaaa
    22441 ccagaaaata ccaacgttgg tggtgaagta gatgtggttt ttaaaacgga caaccgattg
    22501 tagaacgccc ttaattccat cgttttcagt ccagctggta agctgggaga aaatgggtgt
    22561 gtaggatatc ccgttgttta gtgcaatcca aagatttttt tggttatcca tgtatagata
    22621 ccatgcagta ttatcctgta ggccaaccga agtgtcaatg cgtctggtta tgttgcctgt
    22681 agtatcaata aataccactc caccgctgta ggttgaaatg gcaaaggtat tatcatctag
    22741 tttaagccct gcatacacct tggaggatgc gaaaaaatta tcgtcagaag tggaaaattt
    22801 aacaatagca ggctttgtat atccggtgaa atttgggtct agagcttgcc tgctgagcag
    22861 gtaaaagcca tcgttttgag tgcaaatcag aaagtatttt tggttgaatg ggagtattgc
    22921 gtatataagc tgcggggcaa agtaatctcc tcccggaact aggcgcaggg agtcattgct
    22981 caggtagcaa agcccatagt cgcgctggtg gataaacaac acaccatcgg taaggtagga
    23041 gcggtgaaac tgtgtttttg gcgaccattc cgaaacggtt aaatccttgt ttaacctgaa
    23101 tagagtagta aaagatgaaa agtaaattga tgaatcgctg gcgtagagct gacgaatcat
    23161 tccaaggggc ttggccgttt cgggtatatg ctgggaaagg gaatggaagc ttagggtatt
    23221 ggtcgaatcg gctgcaaggt atccaaattc atcctgacca ccaacccaaa ttcttccgtt
    23281 tttgtcagta tcaaggcagc gtgctagtgc tccattaatt gaaatatgtc gccactcgct
    23341 cccatcatac tctataaccc caacgttgtt ggcaatgtat attagccccc ggttatcctg
    23401 cgttattgcc cagctttgag catgcccatt atactcccta tggtgaaagt tcatcaccgg
    23461 aaaccttccg gtggtctgtg cccaaatggc attaacctga gatatcagaa ccagaagcag
    23521 tataaaactt gtacgaagta aatccctttt ttttgccatg cttctcccta ctgcaagtcc
    23581 cactttgaat ttactttcaa atatattgtt ttattcaaat attttattta aaaatggcga
    23641 tgagcgctaa atatatttat tataagggat tcatgccacc gtaacatagt tcaaaatgaa
    23701 tgagtgcatt aaggcaacca tccggaaggt tggaagtatt gaggagcgat ggttgcatat
    23761 tacgcttgct acgggtgcca tgtttttcca gtgctggaac ttgttttggc aaagagatgg
    23821 aatgtgacaa tctgctaaaa aacagcgtat tgacggctta gaagaatttg tactcgtacc
    23881 ggtacacaac cctaaagtta tagtcggagt agtaggtgtc aacttcgtca actattccct
    23941 tgctatccag cttgtagtta cggtgtgagt aatcggtagc gttatcctca taccattttt
    24001 cctccacaat atttccttgc ccatcatact tatatgagct gtagagtagc ctggtattgt
    24061 ttggtttaat caggtaggtt tcaatgagct gattttgtcc atcgtaaaca tagctgtagc
    24121 ttgccctaag ctcgccggcg tagtactccg acttggaaat gggaaggttg ttggcgttgt
    24181 aggtaaatac ggtacgggcg agttccttcc catcgcccga ggtgtttttc tcgtcgagta
    24241 gatttccggc ggagtcgtac tttctttcgg ttattttgct gagggtgcca acgggtttaa
    24301 atattgaaat tttggtgttg ttaccgttat gctcgtatat ccatttttct tctacctggt
    24361 tggcagcgtt gtagcgtata atttcttttt gcttgccgtt gggaaaatag ctgtaggcaa
    24421 tacggtaagt ggttcttcca tcaaaaccaa gttctgaggc tttattccca ttcttatcgt
    24481 aggtaaatac ctgtctgaat gccagctcaa ttcccttttg acggtagtct tggtattgga
    24541 ggtattctat tttacggttt tggttgtcgt acttgtaggt gtggcgcgac gaaacggaac
    24601 cgttttgctt gtagttgatt atttcaactt cattcccatt cttgtcgtag ttggttacta
    24661 cggttacgta gcctttgggg tcaaccttgt ccttgttaaa tttatgagtc cactgtgaaa
    24721 cggaggcaat tttgtttttc tgaattttct cacgactcat gagctcactc cgtgactggg
    24781 cgagtacgat agtgcaggtt gttgccagac agagggttgc taaatatctt ttcattatga
    24841 acattattga gtcaaaagga ctaaaatttt gggaaaggat accagcttaa aaagaagtca
    24901 gttaaggtaa gtaagtaatt ttccacaaag atattcaaaa atttatcaat tttcataccg
    24961 aagttttata ccgatggtta tcccgtgcag gttgagcaag aattttgatg tcgtaatcgg
    25021 cctaccggat tacgcttatg ataagctact gcaaagggtt ggtttatagg ttttcgtcgt
    25081 tgtcgtcctc atcaatttca atggggatgg ttacggccag gataaagaag tggcggttat
    25141 tctcatatgg gacaaagtgg tagtatagct ttcgctttga tttgcgggca atgtcaatta
    25201 gacctaatcc agcgccccct ttttcggtaa gctcaccgcc aatcatttgt tgaagaaata
    25261 aacttttaag ctcggtgggg gaagcgttgt ttaggctgat aaggcgttcc tccaggggtg
    25321 ccattttatc gttccgaatt aggttccccg ttacaatgaa aaaagaatcc ttttcccttc
    25381 ctaccacaaa caacccgttt cctatttgcc tttccttttc gtcatagtcg tcggagtgct
    25441 tggtgatgtt ttgaagacat tctatcatga tgtggaaaac ccgcttacgt attgacgtgg
    25501 ggacatccat atcggagatt ttacggttgg ccataaaggc aaacgaacgc ataatctggt
    25561 ggttcatctc acccttatat ataaggggaa aaccactaga ggcaatttcg cttgccaggg
    25621 tagattttat gaaataaatg atgttatcca ttcaggaaaa tgaaggttga agatatgaaa
    25681 tttcttcatt tcaattcatc tcttggtagg taaatatagg taaataattt atgaatacta
    25741 taaattattg gataaaacgc tcattttatc caacgaaaaa tttatccaac gaatacttcg
    25801 ataattgtac atgtagaaac aggttgtagg ctgatatttt ttatttctgc aggaataagc
    25861 atggtttcgc ctgttgctat tttttctttt ccattgggat agtttaccag gacatcgcct
    25921 gcgaggcaga tgtacagcac aaaggagtct atcatgaagt aatccctttt aaggggttgg
    25981 gtaagggtta gcttgtttac ggtaaagtag gggcagttgg caagctctat gggagtgtta
    26041 aggctttcag taatatttct tttggggttt gaaaccttgc taaaatcaat ggcatccaaa
    26101 gccaggctgg tgtgaagttc acgaggtttc ccagtctcgt ctagcctgtc ccaatcataa
    26161 attcgatagg taatatcgga tgtttgctgt atttcgagta gcaaaacacc ccgcccaata
    26221 gcgtgaactc tacctgcagg aatgtagtaa acatcccctg caagggctgt ttccacgttg
    26281 aggaggtcgg taagtttccc agtcgatagg ctctccaggt actcctctcg tgtaacatcc
    26341 cggttaaatc cggtaatgat tttagcgcca ggatcggcat ctatcacata ccacatctcg
    26401 gttttcccgt aggcatggtg ccgttctctt gccagctcgt cgtttgggtg tacctggatg
    26461 gataggttgt cggaggcgtc aatgaacttt accaggagcg gaagctctat cccaaacttt
    26521 tcataaaccg aattgcctac cagatccccc atgtatattt caaccagctc ctccaggttg
    26581 ttcccctcca ggaagccgtt ggctattacc gaaatattat cgccaactgc cgatacttcc
    26641 caactctcgc cacaatttga aatattcttg ggaatgtttt tgtttagcct ttgccgaagc
    26701 ttgttaccgc cccatattct aggcttgaat atcggtttaa acctgagtgg atacaaaccc
    26761 ttgttctcca tgatgaaagt tttcctattc gattacaaat taattgaaaa tttttaactt
    26821 cgttagttta cctttgtcta ttatttatta acagccatcg tatgcttatt gttggaatag
    26881 ccggcgggac aggatctgga aaaacaaccg ttgtcaaccg tattatagac ctgttgccta
    26941 agggcgaagt ggttgttctg ccccaggacg catactatcg tgatagctca catttgccct
    27001 tggagcaaag gcaggagata aattttgatc atcccgattc cattgagttt gagctgctgg
    27061 tagaacatgt aaaattgcta aaggagggca aggcagttga gcagcccatt tactcctacc
    27121 taacctgcac cagagccaag gaaaccatca ccattaaacc acgccacgtg gtaatcattg
    27181 agggtatatt gatttttacc tgccccgaac ttcgcaacct gctggatatt aaggtttttg
    27241 ttgatgccga tgccgacgat cgcctttcgc gtgttattgc acgggatatt gtggagcgtg
    27301 ggcgctcggt aaacaaggtg ctcgatcggt acgagaaaac agttaaaccc atgcatcttc
    27361 agtttattga gcccaccaag cggtatgccg atatcattgt gccacaggga gggaataatg
    27421 cagttgctat tagcattctt gcttccatta tcgagaagac tcttaggaat cataatcttg
    27481 tgtagccatg cttaaagatg tttctccgga aaatattctt ttcctcgaca tcgaaacggt
    27541 accggcctat ccggcctata ccgatttgcc cgaagctgac cgtaaactct gggataaaaa
    27601 ggcagctcag ctggttaagg atcatgaaac tgcagccgat atttacggaa gggccggaat
    27661 ttatgccgag tttggaaaaa ttatttgcat atcaactggt ctggtcgtct caaaagcaga
    27721 ggagcgattc tttaggatta aatcattttt tggtatcgat gagaaaatcc tactcgccga
    27781 ttttgccgaa atgcttagcc ggtttgaaaa gcgtaaggat gttaacctat gcgcccacaa
    27841 cggtaaggaa tttgattttc cctatatagc ccgtcggatg cttgttaacg acattagaat
    27901 tcccgacata ctcgacgtgt cgggcaaaaa accatgggag gttaggtttc ttgataccat
    27961 ggagctgtgg aagtttggcg acaacaagca ctacacgtcg cttaaccttt taacccatat
    28021 ctttggaatt ccttcaccaa aggatgatat tgacggcagt caggttgccg agtgttacta
    28081 tcttcagaac gatctgaacc ggatagccac ttactgcgag aaggataccc ttgctgttgc
    28141 ccagcttttc atgcggtata aagggttacc acagattgcc cccgaaaacg tggagagggt
    28201 aaatggttag cctttctgca gaaattccaa tatgcttttt gtgaacgcct cgggttgttc
    28261 ggcatgaagc caatgggttg catttctaat agttaccaag cggcaatcag ggtagtaatc
    28321 ggttagccga acttcgctat tctccggtaa atagtttgag ttttctccct ttacgaatag
    28381 cactcgggtt ggggatttct taccgcactc cagatttacc cccgttatca gctcccttaa
    28441 actactttcg agaaccggta ggttaaggca ccagctgtag ctccccgaag cgtctctttt
    28501 taagtttttt agtagaaact gcctcaactt ttggctggga atgctacctg tgagcatttt
    28561 ttcggcctcg tagcgcgact ttattgatgt aagcggaagg cttagcatac ccttaattat
    28621 agatgaatgc tctgcaagcg acaacctgaa aacctcgtcg tttggctggt atggccaagg
    28681 agctatatca gcaacaacta gcgattttac catgttggga aacaacgact ggtagagcat
    28741 ggcggttcgg cctcccatgg agtgccccag caggtgaaag ttgtcaattc ccaaattgtc
    28801 aaccagctcg gctaaatcgt tcgccatatc cgggtatgta tggctgctgg aatgtggact
    28861 ttgcccatgg ttacgttggt cgggaaggat tatcctatgg cttgtgctta atcttttggc
    28921 tattcccatc cagttgtcgg acgaaccata aagcccgtgt agtatgacga gcggaaaacc
    28981 tttgcctagt tcccggtaaa aaagtttcat cagctgcgaa gctgttgcag gtatagctga
    29041 atggtgtttt cgagtccgta gtatagcgca tcgctaatga gggcatgacc aatcgaaacc
    29101 tcaagcaggt tgggtatatg cctgttaaag tagcttaggt tctcaagatt caaatcatgg
    29161 ccggcattaa gccctaatcc cacctgcttg gcagcatcgg ctgcagtaat aaatggctct
    29221 atggcttttt cagggtttac tttaaacatg gatgcgtagg gttcggtgta gagttctacc
    29281 ctgtctgttc cggtatcggc agcatgcttc accatttccg gatcggcatt aacaaagatg
    29341 gaagttcgta ttccctcccg tttaaaagtt gctaccattc gtttcaataa atccttatta
    29401 tttatggtat cccaaccgga gctggaggtt atagcatcgg gcggatcggg aactagggtt
    29461 acctgttggg gctttacgct gagtaccagc tctatgaacg actcaacggg atagccttca
    29521 atgttgaact ctgtagtgat gattggcttt atgtccctga catcctgata tcgaatgtgc
    29581 ctttcgtctg gccgtgggtg aacggtgatt ccctctgctc caaaacgctg gcaatctatg
    29641 gcggctttta gcacatcggg gatatttcca ccccgggcat tcctaagtgt tgcaattttg
    29701 tttatattta cactcaaacg tgtcatacca gaatcttgat atagttaact ttgtgcaaaa
    29761 gtaaccattt ttttcaagcc gcctaccatg atagctctgg agatgatttc ggatgttatt
    29821 cctgccctta aaacttcgga tacgggccaa acagctctaa actggatgga tattttcaaa
    29881 gtttcacacc tacccattgt taacaacaag gagtttttag ggttggtttc agaatcggac
    29941 atttacgatt tgaacatgcc cgatgaaccc ctaggtaacc attacctatc gctaaatagc
    30001 ccttatgtta ttgaggacca gcacatattt gaagttatgg aagtgctttc gcgtctgaag
    30061 ttatcgctgg tgcccgtttt agatagcggt aagaactacc ttggcgtgat taccctgatg
    30121 gagctgctgc actactttgc cgtagaatcg ggaataagcc tgcagggggg catagtggtg
    30181 ctggaaatga atgccaacga tttctccatg tctcaggttt gccaaattgt tgagggaaac
    30241 gatgcaaaaa ttcttgccgc ctacgttacc ccgcacccca actccaccag gatggagctt
    30301 accctaaagt tgaatgttac cgatttgacg tctatcaggc aaacgttcaa caggtacaac
    30361 tacaccgtta aagcttcgtt tcttaagcag gatgatgagg ccgacatgct gaacgatagg
    30421 attaattttc tttttcgcta tctcgacata taaactaaac aattcgaccc tatggtggtt
    30481 gcaatttatg gaaaagcggt tgaaacggag tatcttccct ccttgggtga actttttaac
    30541 cagctggttg agcataatgt agaggtttgg gtttacagac ccttcatgga gtatctccta
    30601 gaaactttct cggccaagct gcctgacttt gaagttttta ataccggtag cgatataaac
    30661 gggaaggcta acatacttat cagcattggg ggtgatggca cgtttcttga ttctgtttcg
    30721 tttgtccggt catctggctt gcctattatg ggcattaact ttggtaggtt aggttttctg
    30781 gccaacattg ccattcccga catgcacaat gccattgatt tgctgattgc agggaggttt
    30841 aaagttgaac atagaagcat gctggaagtt gtttcaacgc tggagcaatt taacactttt
    30901 ccatttgggc tgaatgattt taccatgcag aaagctagtt cgggatttct taaggtgaac
    30961 gcttacgtaa atggggagta cctttgtgcc tactgggctg acgggcttat tgtctctact
    31021 ccaaccggtt ccacggccta ctcgcttagc gtaggcggac ccattgttag cccaaacctt
    31081 tctgccctaa tcctgtcggc tattgcacct cataacctta ctgtgcgtcc gttggtaatc
    31141 agcgatgaca acatcctact gctcgaggct gttggacgtg atggcagtgt tatgctatcg
    31201 ctggattccc gaagcataac atgtaagtcg ccggttaccg ttaccgttaa gaaagctcct
    31261 ttttttgcca atgttgtcag catggaggga tcgaacttct atcataccct taggggaaag
    31321 ttgctttggg ggatggataa acgaaactaa tggcagctaa agctgttgga ttgcatggaa
    31381 tggaattttt aaatagttaa ttgttatata tttgtagttg ataatactgg ctaatgaaga
    31441 aatcctttat agtggtaggg ataattcttt ttctttgtct gcctggccac tcccaggtat
    31501 ggaaacgaaa tagaattgaa atatttttgg gagttcccat aacccattac tttggagata
    31561 ttggtgggac cagcgatcct agcagtatga tggggataaa ggatatcagc atcagggcaa
    31621 tacgacctgg tttctcggga gggttgggtt acaggctaac tgagcgtttt tatgcccagg
    31681 gtactttaac tctaggcctt tttggaaact cggacaaggg ctctaggaat gaagcacgca
    31741 actacgcctt ttctactttt ggaaccgaat tagcggcagt tgcgcagttc tacattatac
    31801 ccgagagcga ccagaactac ttctacagca tcatgcaggt tcgcgggggt ttaagaaaaa
    31861 ttaacaaacc ttttagcctg tacctttttg ccggcggtgg tggatgtttt tatagggtta
    31921 aaccccttaa tgaccttgtt gattccgata gatttgacga ttcacagcat acagcagtcg
    31981 tagtaccttt tggtctgggg tttaaatatc aaattcattc cagaatgttg cttggagttg
    32041 agctgggagg acgatatgtg ctatctgact accttgacgg attttcacca gaaccctcaa
    32101 aatataatga cttctactac gtttttaact tcaaagtgca ctacaggctt cccagtaata
    32161 aaattttaag aaagccccaa tggacattct agctaacagg atatttttca gttttttgtc
    32221 aagtttactt gttctttcat tttttttgtt gttgcccttc aaatctcacg gccaggataa
    32281 aagggatttt ggtgttcagg cgggaagcac atactactat ggcgatttca acgaggcaaa
    32341 gccgctctac caaccctcct ttgccatggg ggttattttt cgatacaact ttaacacgca
    32401 gtactccatt agggcttcag cgttgtatgg cgcaattaag ggagaatcac catccggttt
    32461 ttttttacct ttgtccgcct ctactacttt ttctaacacc ttacttgaag cggaggtgat
    32521 gggagagttt aattttgtta gctttagccc tttaagcacc aggaaaaaga aactctctcc
    32581 atatgtaaac attgggttag gcgttggcca gctaagtggg tctgttttgt tccatatccc
    32641 ttttggggta ggacttaagt tttcacccgg taaacggcat acgttagcga tggagtggcg
    32701 attccataaa acattcaacg atgagctgga tagctatgct gcacctgatg atggtaaaac
    32761 accttttcta cacaataacg actggttctc gtacattggg ctaatataca cctataggct
    32821 ttacaactca ggcaatgttt gcccggtgta taagtaactt ttttttacta tggacattaa
    32881 aaatcagatt gataagaaca acgttccgca gcatgttgcc ataattatgg atggaaacgg
    32941 acgctgggcc gagaaacggg gcctacccag aatttttggg cataaaaatg gtgtaggtgc
    33001 agttagggca gccaccgagg ctgctggcga aatcggcgta aagtttctta ccctttacgc
    33061 tttttccacc gaaaattgga aaagacccaa aattgagatt gataccctca tgaacttgtt
    33121 gattgaaacc attgataatg aaataggtaa tcttaataag aatgacgtcc gcctcattac
    33181 cataggaaac acatcggccc ttccaaaaaa cgttcaaacc aagctaaaat atgccataga
    33241 taaaacctcg caggggaaaa ccctgacaac cattttagcg ctaaactatg gggcaagagc
    33301 cgaaattacc gaggctacac gaaaaatcct taccgaatac cggaatggaa aacttgacga
    33361 gatgagccta tcgcctgaag tttttggcgg gtacctgcaa acagcgggaa tacccgatcc
    33421 ggaactactc atcagaacta gcggcgaaat tcgccttagc aattttctac tatggcagct
    33481 agcctacgcc gagctctact ttacccctgt cctctggccc gactttagca aagacgattt
    33541 ttttaatgcc attgttgagt accagcacag ggaaaggcgt tttggaatga ccagcgagca
    33601 gctttagctc aattcgcatt ttttagcttg ttccctgagg gtcatttacc tacttttgaa
    33661 cccagcctct tatttgtata tagaactttg gcaattgaaa atatattatt tttacacccc
    33721 agtattaatt agcaaaattg cagtatgcta aaatcaaaaa tatttttact tctacttctt
    33781 cttagcagcg tttccgctct ggctcaggag ccctcgttca gctacgaggt acctaaaaaa
    33841 tactgtgtca aagaggttag ggtgtcgggt atcaagtttc tcaatcccga tgtgctggta
    33901 tcagtttcgg gtattagcgt tggcgattca atccttgtcc ctggtgatgc cgtaaccaag
    33961 gctattaaaa agctatggag ccagggtcta ttttccgata ttaaaatttc ggctacaaaa
    34021 attgaaaatt cagatatcta cctcgatatt tttcttcagg agcaaccccg tatttcgtct
    34081 atcaactttg ttggagtgag aaaaggtgag gttagcgacc ttaaggagaa gttgaagcta
    34141 cgcaacggaa gccagctaac cgagagcatc cttgagaata gcgtggagat tatcaggaag
    34201 cactaccgga ctaaaggatt tctgaatgta gatgtaaatc ccattcagga aaatgatacc
    34261 gctttggcca acatggttaa gcttactttt gatataagaa aaaatgaaag ggtgaaaatt
    34321 gaccaaatta catttgacgg caacacagca tttaccgatg cccgcctacg taaatccctt
    34381 aaaaaaattc accgccgtga tatcaacatt tttaaatcgg caaaattcat acagtccgac
    34441 tacgacgaga gcaaggataa cctgatagca ttttacaacg agaatggtta ccgggattcc
    34501 aaaattttga aggattccgt ttttacattg aacaacaagc gaattggcat taacttcctt
    34561 gttcacgagg ggccacagta tcacattcgc agcatttcct ggattggtaa caccaagctt
    34621 ccctccgaag cccttactgc catactggga atgaagaagg gcgacgttta cgataaaacc
    34681 cttctggaaa agaggctttt caccgacgac aactccattt cgaccgttta catggatgat
    34741 ggctacctgt ttttcaacct cgatccggtt gaaagccaga ttcagaacga ctccgttgac
    34801 ctggagatga gaatatacga gggcgaccag gccaccatta acaaagtgct gattgttggc
    34861 aataccaaaa caaatgaaca cgtaattcgc cgtgagcttt ggaccaaacc gggctacctg
    34921 ttcagcaaat cggaaataac acgcaccatc agggaacttg gccagatggg acatttcgat
    34981 cccgaaaaac tcgacgtgca gcccatgcca aatcccgctg agggaacggt cgatcttaaa
    35041 tacgtggtgg aggaaaaggc caacgaccag ctcgagatat ctggaggatg gggtaacaaa
    35101 atgtttgttg gaacggttgg cataaggttt accaactttt ccgttcgaag actctttgac
    35161 aaggaggcat ggaggcctgt tccttcgggt gatagccaat ccctatcact tagagcttct
    35221 actaacggaa cctactacaa agccttcagc ttctccttta ccgaaccctg gctgggtggt
    35281 agaaaaccaa ccaacttttc cttctcggtt taccacacca tccagaatgg cggagtggac
    35341 tacttctacc agacgagtga taaatatttt aaggtaaccg gagcttctgt gggtattgga
    35401 acccgcttga aatggcccga cgactacttc agcatttaca acgaaattag ctaccagaac
    35461 tataacctga aggattggac gggatacttt cttttcagca acggccagtc gaataaccta
    35521 agctacaaga tttcccttac gcgcaactca accgatcagg ttatctaccc ccgtacagga
    35581 tccaacctta gcctgtcact tcaaattacc ccaccatact cgctttttaa tggaaagaac
    35641 tactcggcat tttccggaag cgaaaagtac aaatggattg aataccataa atggaccggg
    35701 cgaattcaat ggtatgttcc cctggtcgaa aagctggtgc tctactccaa cttccagttc
    35761 ggaattatgg gttactttaa cagcgatatt ggccattcac cctttgaggg atttgatgta
    35821 ggcggtgatg gtatgtcggg ctacaacctt tacggcaagg aaacaattgg cctgcgtggg
    35881 tacaagaatg gttctcttac cccgtacacc ctgatagatg gtagctacgt tggcgacggc
    35941 catgtttacg acaagtacac cgttgaaatc cgctaccctt taacccttaa cccacaggcc
    36001 gccatatacc tactaacctt ttttgaggct ggaaatgcat ggcgtgatgc cagcgagttc
    36061 aaccccttca acgtgcaccg ctcggcaggc attggcgcaa gaatgttcct tcccatgctg
    36121 gggatgcttg gaattgactg gggatacgga ttcgataaaa ttcccgacaa tcccgacgcc
    36181 cataaagggc agtttcactt tattatcgga atgccatttt aacaattctt aacccttata
    36241 tcaaccctat ttcaataatt ggcaggcatg ttgatatatc cttaacaatt cagcttcaac
    36301 ttgaaatact tagccgatta cttttgatac tttaaatatt tgaaaatgac cagcttaatt
    36361 ttactcgcaa ccagtagtcg catttaagtt taaataaatt acacaccaat gaaaaaaata
    36421 ctcctactcc caattgtctt gctgattagc acaagcttgt gggcacaaaa atttgctttt
    36481 gtcgattcag agtacatcct caataaaatt ccttcctata aggcagccca ggagcagctt
    36541 gataagctct cggagcagta ccaaaaggag attgaggaca agtatgctga aattgacaag
    36601 atgtataagg actaccaaac cgaaaaggtc cttttaaccc aggaaatgca aaaaaaacgt
    36661 gaagatgaga taatttccag ggaaaggcaa accaaagagc tgcagatgaa gtactttggt
    36721 cgcgatgggt tactctttaa gaaaagggaa gagctggtta aacccattca ggatcaggtt
    36781 tttaatgccg ttaaggagat agccatcgaa ggggggtatg ccattatttt cgatgctgca
    36841 gcatcaccca acatgcttta caccaatccc cgctacgata aaagtgaaga tgtgctccta
    36901 aaattagggt ataaataatt taatttttat atttgcttac ttaaaaattg ataatcatga
    36961 gaaagatatc cacgattgct ttcattcttt ttttatcagc cactggtgtg aaaacctatg
    37021 cgcaaagcta taagtttggg cacatcaaca gccaggagat tctaagtgtt atgcccgaga
    37081 gggatagcgc tgttgccaag ctggataagt ttaccaagac gttgcaggat caggttgagg
    37141 agcttcaggt tgaatttaat aagaagtatc aggattacct tcaaaagcgc agcaccttta
    37201 ctgacgccat ccgcgagatg aaggagaagg agcttaccga gatgcagcaa agagctcagg
    37261 agtttcagca ggtagccgag caggactatc aacgctacca gggtgaggtt atgaaaccgg
    37321 tcattgaaaa agcagattct gcaattaaaa aggttgccaa ggctaacggg tttacctaca
    37381 tatttgatac cagcaccggg gtcatccttt acttttcgga gcaaagtgtt gatataggtc
    37441 cattggttaa aaaggagctg gggataacga aataatcagc cctgatggct tatactaggg
    37501 atttgccgac gtatgcggtt tatccctttt ttcatttatt tatattttgt tcttctatgg
    37561 ctcagaaggg taatccatgg agtaatcgtt agcatctgcc taggctattg tcatgaatat
    37621 ttcatttttc ccaaattaag gttatcgtta attgcttggt agtaatccta taggaatttt
    37681 tgactccggg tcaggcggat taacggtgct gtatgaactt ttaaaagtac tgccccggga
    37741 atcctttgtt tactttgccg attcggcaaa ttgcccatac gggcccaaac cccagtcgga
    37801 ggtgattgaa ctttctgact gcattacccg ttttttgctg gaacagggtt gcaagctggt
    37861 ggttgttgca tgcaacaccg caaccgctgc agctattgag caccttcgaa gtaactttaa
    37921 aattcccttt attggcatgg agccggctat aaaacccgcc tcacttaatt ccaaaaccaa
    37981 gagtattggc gtgttggcaa ctgccggaac ttttaagggg aaactttaca tcgaaaccag
    38041 taaaaaatat gcctctgatg tgaatgtctg ctaccaggtg ggagagggtt tggttgagct
    38101 ggttgaggcc ggcaaggccg attcgcccga agctgccgag ctccttcata ggtatgttga
    38161 cccaatggta gattgcaaca tcgaccatct ggttttaggg tgcacccatt accctttttt
    38221 gatgaactcc ctaagccgga tactgcctag aggggttgaa attgttaatc ctgcacccgc
    38281 tgttgcccgg cagacacttc gcgtgctttc cgaaaacaac cttttaaacg atggtagctc
    38341 cttgagcaag gtaagcttct actcaagcgc ctccgtggaa attttgaaat tcctcgccga
    38401 aaaactcggt gtaatttcta accatggacc ggcagtcaac tttaccggag gtgtaaaact
    38461 ttgctgtcgt taatgcacct attttattgg ttcttaaact taccctacta catatgagaa
    38521 gagaagttga aattgtgctg gagaacacta accaaaaggt tcggataacc ccaggtttgt
    38581 cgcttatgga gctaaccggc atattcaata tcaagctgga gcaccaggtg ctgggcgcta
    38641 tggtaaacaa ccagctgaag gagctggact acgaaatttt tactcccaaa acaattagat
    38701 ttattgatat tacccatcct gcaggaatgc gtatgtacca acgctccctt tttttcctgc
    38761 tgcataaagc tgtcggggac atactgcccg gctgcaaggt aaggattgaa cattctgttt
    38821 ctaagggatt ttattgcgag atcgatggct tcgagcagcc gcttgacttg cccctggtgt
    38881 tttcaatttg cgacaaaatg cgcgaggttg ttgctgcaga tatcccattt cgaagagata
    38941 aaatccttac caccgatgca gtaaaactct ttgaagatct gggctacacc gagcaggcca
    39001 agctctttga cacccggcca aaactttaca cctcaattta ctacctgggt gatttacccg
    39061 gttacttttt tgggaatttg gttccttcaa ccggctacct aaaagtattc gacctggtta
    39121 agtactacca gggtatgctc ctccgcattc caaagcgttc aaagcccgac gaggtggagg
    39181 atgtagttat tcaaaataaa atgttcgata tttttcagga gtataaggac tggactcata
    39241 ttctgggggt aggtaacgtt gttgctatta acgaacaggt tgaacaggga aatgccagcg
    39301 agctgataaa ggtttccgag gccctacatg aaaaaaaggt tgcctacatc gccgataaaa
    39361 tccactcgct gggcgacaaa gttaaagtta tcctaatttc tggcccatcc tcgtcgggca
    39421 aaacaacatt tgccaagcgg cttgctgtgc agttaagggt aaacggcatc agaccctaca
    39481 ccatctcgct tgacaactat tttgtcgatc gggatagaac cccccgggac gagaatgggg
    39541 atttcgactt cgaagcatta gaagcgcttg atatagaata ctttaacagc gacttgctca
    39601 ggttgcttaa cggtgaaacg gttgagcttc ctaaattctc gttcgagaag ggcgcccgat
    39661 tctttgataa caccctaatg agaatcgatc ctaacggcat gattatcatt gagggtattc
    39721 atgcccttaa cccaaagctc accccgttaa tcccatccga caaaaagttt aaaatatacg
    39781 tttcagcgtt gacctccatg tcgtacgacg gaattaaccg tattccaacc accgataaca
    39841 ggcttatcag aaggattgtc cgcgactacc ggtacagggg ttacaacgcc cttcaaacaa
    39901 tcgcccgctg ggagagtgtc cgtaggggag aggataggaa catatttccc taccaggagg
    39961 aggctgacga aatgtttaac tctgccctgc ttttcgagtt tggccttctt aagagctatg
    40021 ttgagcccct gcttcaggaa gtgccgccca atagacctga atttgatgaa gccgaccgcc
    40081 tgcttaaatt cctttactat ttcaatacca tttcagacag cgaggttcct ccaacctcga
    40141 ttataagaga atttttaggg ggtagcgctt tctcctactc ttaaaatgcc atgttaagtt
    40201 attaacatgc tatatagttg aaaatcaatt caatgaaatt attttgttta tatatttttt
    40261 gccttaacct gctgcttccc atgattttta ccttgttctc ttgcttttaa ttataattat
    40321 tcactataat tgtaacaaat taaccctaaa cctgaatcca agctataatg gcaaggctat
    40381 ttatagttat tatcaacata ataggtatta cgcttgctgg cattttggga ggtggtaatg
    40441 tgctggttac catgaaagct ccaagtcggg tagatgcggg ttcggaattt gatgtggaag
    40501 tgctcctaaa aaaggctggt gttgatggtt ttgcccgatt tcagcaggaa ctacccctag
    40561 gcctcactgc tgaactaata gaatcatccc ttggcgattt tacttttgag gatcaaaagg
    40621 taaaggtagt atggcttaag cttcctgcag ccaaggagtt acgatttgtt taccgaattc
    40681 gggttgacga gcgtttaaag gggagtttca accttggtgg cgttttctca tacattgaaa
    40741 ataatgagcg taaaacttct tccgtgcagg cagcacagat ttctatcacc ccttcaactc
    40801 gtatcaaccc ttcgctgata gttgatatca acgagtttca gaatgtcatt cccgttcaag
    40861 cccctgtttc tatgctggct tcgaacgtta agtgcattag gcaaacccct atacccaccg
    40921 gcgaggctaa cgatttgctg gtaaaactat tggttaataa gggtaacgcc cagaaatttg
    40981 caaagattga ggaggatata ccccagggct ttcaagcaga ggtggtagat gccaaggatg
    41041 ccatttttac ttttaaagat caaaaggtta aatttctatg gatgagtatg ccgcccgacc
    41101 ctcgcttcat tgtgtcgtac cggctaattc ctattaacgg aaacggagaa gccgttgtca
    41161 gcctttcggg aaaattttcc taccttgttg gtgaggctac acgggtagtt gacattatcc
    41221 aaaaggatgt tgacttgggc aatctcgacc cgaaatccat cgagggattg ctggcctcca
    41281 tcccgggcgc tgaaattgca gctccctctt cttcaagctt cagcagcagc tttacatctg
    41341 ctggctccga tggtggaata gatattcctg ttcagtacca ggtaattcca gagaaatcaa
    41401 aaaaaatagc ccagcccgaa caaaacatga tgctctacat gctggagcct gaaaaagggg
    41461 tttactacag ggttcaggtg gctgccgggc ataagcccat caacatcaaa aggtattttt
    41521 tacgacataa tgttacctac gaggtgcgaa ccgagaagca tgagggttgg tataaatact
    41581 ccataggatc gtttaaggag tacaaggaag cgcgcgactt tagggtgcaa atatggaata
    41641 ccactaaaat taacgatgcg tttgtggccg cctacaacaa cggtgttaga attaccgttc
    41701 aggaagcact aatgataacc aaccaaactt ggttcagata gctagctgtt aaagttctga
    41761 tgagaaggtt tattgccaga aataaaatta cactctttac cctctgcatt tcgggtatgc
    41821 tcctttgcac ctataattca gcccatgcac aggatgatga ggaatactac aaaaacttgc
    41881 tcgatacggt tgttgaggtt attaaccccg tttacaaacc ggttctatca tttggcgcag
    41941 gaatcatgag tttttggggg gacgttcaaa accctgtaac caatcctgtt aacggaaagt
    42001 atggtttcaa atttaacgtt tcaacgcttt ttgggaagaa ctactttaag ggaaatattt
    42061 ttgcgattta cggccagttt cagggtcaga actttgatat tacccgtcag atgcagctac
    42121 aaccattacc cctagacgat attggaaatc ccatttatcc aaactcctca ttccaaaccg
    42181 agttctggca ggttggcata actgctgagt atggtttcgg tcatctattt gggctggtta
    42241 agcgcttcaa accatttatc gcagttggcg tttcaactat gttttttacg cctaagggaa
    42301 atgtcaggta tgggcctacc ggtgacttct actatgcttg gtccgacggt tcgcttcgtg
    42361 atttccccga aaatggtcct aatgcctact tagctcaaat aatcaaactc gataatagct
    42421 acgaaacaga cctttccagc gccgatttgt acagaattgg ttcctacagc cagaatacct
    42481 ttaccattcc acttgacctg ggtttcgatt tttacctgtc cgacagggtt aactttaggg
    42541 taggtgctac cgttaactat gccttttcag atttgctgga caactatagc agcaaaactg
    42601 ccagcaccat gggctacccc aataaaaaca gctacaacga tatattcgca ttcacctatt
    42661 tcagcatgaa cctcgacctt ttctccgacc caaagaccat gctggtcgag agggtgtttg
    42721 ctgaacttag tggtacattt gactacgggg ttttctttgc cgatcaggat ggcgataatg
    42781 tgttggatag gtttgatgca tgtcccgata cacctccagg agtggccgtg gattcgcttg
    42841 gttgcccacc cgattatgac aacgacggag tattcgacta tatggacgat gagtccagta
    42901 cccctgaggg agcaattgtc aacgagaggg gagtgcagct ctcggctgat gtgctttccg
    42961 atatgtttag caaaaactcc atggcagtgc ccagaagtga ggttaaggtt attcccctcg
    43021 caaatgtctg gacacgaaac gcaaccttta ctcctggtgc aattcccgat aaattccgca
    43081 tcgttgatgc agatgctgat ggatatatct ctttccagga gctgctcaag gcaattaacg
    43141 acttctttga tgagaaaaac acctttaccc ccgatgaaat ttatgagctc aacgaatact
    43201 ttttctcaca gtaagcaaaa acctaaaacc aattaaccta taaacccaaa caaccctata
    43261 taacctaacc taaaaccctt ttactacctg ccctacagaa ttcctgtagg gtttttttta
    43321 acttcagggt gataaatttt gtcaggaaca agcaaatgaa ggttacggta ttttctgttg
    43381 ataagtcttt ggtagatttt ttgaacaacc cgagagctat tttgtatatt cttcataact
    43441 ttacaaaatc aaagtgatga aaacgacctt tctaaaacct ggcgatacag ctcctgactt
    43501 tacagcagtg gatcaggatg gcgttgaatt cagcctttcc gagtaccagg gcagagcagt
    43561 agtgctttac ttttatccca aagataacac aactggctgc accgaagagg cttgcagcct
    43621 aagggatggg tacacggaac ttaaacagct ggggcttgac gttatcgggg taagccccga
    43681 cagcaccagg tcgcataggt catttatcca gaagcacagc cttcccttca ggttggctgc
    43741 tgacaccgac catcggattg ctgagttgta tggggtttgg gctgagaaat caatgtatgg
    43801 caaaacttac atgggaattg ttcgcaccac cttcataatt aatggcgacc ggaaaattac
    43861 ccatgttttt gacaaggtca atactaaagc ccatgctatg caaattttag aggagttgaa
    43921 aaaaacgctt taacatacca accacatgga aaaggtagag aaagaaaaag aaaagaaaga
    43981 gcgcaaggag ataagtcagg ataagctgaa ggctcttcag tcaaccctcg acaaaattga
    44041 aaaagatttt gggaaggggt ccatcatgaa aatgggtgat aaggcagttg ctgatgttgc
    44101 ctcaatatcc tcggggtcaa ttgctctgga tatagcgctt ggcattggag gataccctag
    44161 aggcagggtg gttgaaatat tcggacccga atcgtcggga aaaacaaccc tggccattca
    44221 cgccattgcc gaagctcaaa aacttggtgg cattgccgcc ataattgatg ctgagcatgc
    44281 ttttgaccgc tcatacgccg agaaactggg agttgatgtg gaaacactgt tgatttcgca
    44341 acccgacaac ggagaacagg cgcttgaaat agccgataat cttatccgct caggggcaat
    44401 tgatataatt gttatcgact cggtggcagc ccttacccct aaggcagaaa ttgaggggga
    44461 aatgggtgat agcaaaatgg gccttcaggc aaggcttatg tcacaggccc ttcgaaagct
    44521 aaccggaaca attagcaaaa ctaacacctg ctgcattttt atcaaccagc tgcgtgaaaa
    44581 aatcggcgtt atgttcggaa atcccgaaac cactaccggg gggaatgccc ttaagtttta
    44641 cgcttcggtt agggttgata ttcgtaagct caaccagctt aaggatgggg aggaatcaac
    44701 cggtaaccgg gtgagggtga aaattgtaaa gaataagctg gctcctccgt tcaagaaagc
    44761 cgagttcgac atcatttttg gagaaggaat ctcacgaacc ggtgaaattg ttgatcttgg
    44821 tgtggagtgt aacattatta aaaaaagtgg atcgtggttc agctacggtg aaacaaagct
    44881 gggacaaggt cgcgatagcg ttcgccagct cctacaggac aatcccgagt tggccagcga
    44941 aattgaggcc aaaattcgcg aaacgttaaa gggttagcgg tcaggtagag cgtttggcaa
    45001 caaaaatctg atatttttac cactcctatt gatgcagaac tttgttactg cataaagtta
    45061 accccgctgc ctggcagcgg ggtttttttg ttggctatat acctatctcg gtatccttgg
    45121 agtcaaaaaa atggtactcc agaaagtggt aggacgacat gggagttaca tttatcctgc
    45181 agtggtacct aaacgaccac cgcatacgct ttgaaacaaa acccttgttt aggaaagctg
    45241 caatgtaggg gtgcagctta agggtaatac tttttaactt gtggctggta accagcgtgg
    45301 aaagttgatt ttcgatctgc tcgtcgagca gaacagtggg gctaacctct cctgtcccct
    45361 tgcacgtggg gcatttctcg gtagtgttga tatgcatttc gggacgaact cgctggcggg
    45421 ttatctgcat tagcccaaac ttgctcaacg gtaaaatgtt atgcttagcc ctgtcttttg
    45481 acatcagctc cttcatccgc tcgaacagca tgttgcggtt ctcgttctga tgcatatcta
    45541 taaaatcaac cacaatgatg ccgcccatat cgcgaagcct gagctgacgg acaatctcat
    45601 cggccgctgc caggtttaca tccagtgcgt tggtttcctg atcgttacca gattttgaac
    45661 gatttccgct gttaacatca attacatgta aagcctcggt gtgctctatg atgaggtatg
    45721 ccccgctttt tattgaaaca gttttgccga atagcgattt tatctgcttt tcaatcccaa
    45781 actggtcgaa tatgggaaca tttcccgtgt aaagtttgac aattttctcc ttttcagggg
    45841 caatggtttc aatatactca tgtatatcgt tgaacacaat ctcgtcgttc acatgaatgc
    45901 tgttgaacgt tccgttcagc atatcccgaa ggatggccga ggttcgcttt agctcgctgg
    45961 ccagctggcc ggggaaggat actgttttta acttttcggt tgcggtttcc cacttcttga
    46021 taaggctgcg aagctcggca tcgagaactg ctaccttctt gccctcggca gcagtgcgca
    46081 caatcacccc gtagtttttg gggataatgc tttccatcag ccgctttagc cttttgcgct
    46141 cctcgtttga ggaaatcttc tgagaaatgg atactttatc cgaaaatgga atcagcacga
    46201 ggttccgacc ggcgatggat atttcggagg tcaaccgggg gcccttggtg gagatgggtt
    46261 ccttggctat ctgtacaagc acagcctgac ccgatgaaag tacatcggta attttcccgt
    46321 tcttatctat atccggttca agcttaaatt tgggtacggg tatcgtttta gtccttcggg
    46381 cgtagtgggc ttgaacatac tgctgaaggg ttttaaactg cggacccagg tcgaggtagt
    46441 gcaggaatgc atccttttcg tagcctacat ctacgaatgc ggcattcaat ccgggcatga
    46501 ttttttttac cttaccgaga tagatgtcgc caaccgaaaa ctgaacgttg cctttctcct
    46561 tgttgagctc taccagctgc ttgttttcga gcagggcaat ggcaatttca gcttcctgta
    46621 catcaattac tagttcggtg ttcactttaa ataatttagg taaggtatat acaacaaata
    46681 gagcaaccta aagcgccatg caggaacttt aggttgtttg cataatttcc tgattcctac
    46741 taaaccaggg ttgctacttc tttttcttat gacgattctt gcgtaaacgt tttttgcgtt
    46801 tatgcgtagc cattttatgc ctttttcttt tttttccgct tggcatgacg tgaaattttt
    46861 ttggttagta tttatggttg cctatttctt aacgtgattt ttcaccttat ttacaaaggt
    46921 cttggcgggt ttaaacgatg gaatgtagtg ttcgggaata attatggtgg tgttttttga
    46981 gatgttacgt gcggtttttt gtgcgcgctt tttaacaata aagctgccga accctctgag
    47041 gtaaacattt ttttcctttg ccagggagtc cttcactatt tccatgaagg cttctacaac
    47101 cttctgtgct tctacctttt ctatcccggt agtcttggaa atttcattta caatatctgc
    47161 ctttgtcatt tctaaaagta tttaattatc aatatgttac acatagtgga tgatttcgaa
    47221 ctgcaaatat aaacctttta tctcgataat tgaaacggca agtattaatt ttgacaagaa
    47281 tttgttttgc taagtgctga ttgtatggat tttagtgcat tgctcctgga atggtttagt
    47341 gtacaccatc gtgatttacc ctggaggaat acgtccgatc cttaccatat ttgggtttcg
    47401 gaggttatac ttcagcaaac aagggtaaat cagggaacag aatactatca aaggtttatt
    47461 catattttcc ccgatatacc agcattggcc gaagcgcccc ttgatgctgt tatgaaggca
    47521 tggcaagggc tgggatacta cacccgtgcc cgcaacctgc atgcaggtgc caaacaggta
    47581 atgtccgaat ttggtgggaa gctgcccacc agctactccg aacttctgcg tattgccgga
    47641 ctcggaccat actcagccgc tgcagtggct tcgtttgcct tcaacgaggc tgtgcctgct
    47701 gtggatggaa atgtttacag aattctggct cgtgtgtttg gtgtgtttac tccggttaac
    47761 tcctcacagg cccgaaggga gttttttaac cttgctggtg agcttattga caggaatcgc
    47821 cctgctgaat ttaaccaggc tattattgac ctgggtgcat tggtttgcac accgcgcagg
    47881 ccttcgtgtt cagcctgccc tttttccgac tactgctatg cctatttaaa caacctgact
    47941 gggttgctcc cccttaaaaa caggaagaat ccaccccgat cacgatactt cacctacctt
    48001 atgattaact ttaacggaac aacctttatc cgcaagcggg aggggaacga tatatggcac
    48061 tccctgtatg aatttccgct cattgaaact ccagaacaac ttgataccac tcagctggtt
    48121 gaaaccgaag cgtggaaaga gctaatgggc gattcatcag ccgagattat tcatgtgtcc
    48181 gacccaatca agcatcagct tagccatata accctgcatg cccgttttat tatcgtgcaa
    48241 atgagaaacc ccacctatgc cttacgaacc ggatatcgaa acattagcat tggaaaccta
    48301 caagagttgt cggtaccgcg tttaattgat tcatacatgg cttctgaacc tgctgaacgc
    48361 tactttacgg gaaagtgaaa tttatttgct ttgtatattt ttttacccta ccttggtcta
    48421 aataaccaaa aatctaaaac aatgtcagtt aacaaggtaa ttctagttgg caacgtgggc
    48481 aaagaccccg atgtacgcca cctcgaagga ggagtaactg tagcccgttt cccactggct
    48541 accaacgaaa gctataccga caagtccggg aaaaaagtta cccaaaccga atggcataac
    48601 attgtggtat ggagaggttt ggccgatgtt gctgaaaaat atgtcaggtc tggaaagctg
    48661 ttgtatgttg aaggtcgcat tagaacctca tcctacgacg acaaggaggg aaataagcga
    48721 tacagcacag agattgtttg cgacaacttt cgattccttg gccccaatgt gaaccagcag
    48781 gaacattcca ccccttaccc tgagcaagtg tctgatgcca aatccaacct ctccgacgtg
    48841 gagaacattc cttcgcccga tgacgacctg ccattctagc tgcttattat ccctgcccca
    48901 aaaaccgata gcctaaccaa actatcggtt ttttgttggt aaatggctgc tgtatgctaa
    48961 attaacgtaa tctctgaata ttttagtaat tttacaagtc gaagccatcg cagaatggcg
    49021 aatattaaaa ggagttggat taatggatat tgcctttcat cctattactg ttggaacggt
    49081 agtcggcctg cttgtgttga ttcttttgct tatcatttcc ggcctgatga gcggaagcga
    49141 gtcggcctac ttttcactct ccccggccga tttgaaaaag ttggaaagca aatcgcacca
    49201 aagggctcag agtttcgtgc tgatcaacct taacgaatcc gagaggttgc tagctacaat
    49261 tctgattgtt gataacctag tgaatgttgg aattgtcatc ctttccacct acctcactaa
    49321 ttctttaatt gatttttcga atgctcccgt tgccggtttc atttttcagg ttattgtcat
    49381 cacctttatt ctgctgcttt tcggcgaggt tatgcctaaa atacttgcta caaaccgtgc
    49441 gcttaagttt gccctgttta tggcctatcc ccttcatctg cttgttaggc tgtttaggcc
    49501 ccttagctcc ctactgctat cttcaacctc tagaattggg aaacgtgcga gcacccataa
    49561 ccagatttcc atggatgagc taagcaacgc cctggagata acatccgata gcattgccga
    49621 ggagaaacaa atccttcagg gtatcgtaac gtttggaagc accattgtga gcgagattat
    49681 gaagccccga attgacgtag tggcggtgga gtacgacact tcgttcaacc accttaaaaa
    49741 cgtagtggtg gattcgggct acagccgcat ccctgtttac tccgaaacct tcgatagcat
    49801 tcgtggcata ctctatgtta aagacctgat cccctacatt gaccgtgagg acgactttca
    49861 ctggcaaaac ctgattagaa ctccctactt cgtccccgag agtaaaagaa tcaacgactt
    49921 gctatcggag tttcagctga aacgtatcca catggccatt gtggttgacg agtatggtgg
    49981 aacatgcgga ataatcaccc ttgaggatat tcttgaggag attgtaggcg aaatttctga
    50041 tgaatccgac gacgaaactc cgctttacac ggtagttgac agctcaacct atatttttga
    50101 gggcaaaatt ctgctcaacg acttctgtaa ggttattggg tgcagcgacg atgtgtttga
    50161 tgacgtaagg ggcgaggccg agactcttgc agggctcatt cttgaaatta ccggacaatt
    50221 tccttcccga aacgatgtgg taacctgcaa acagtttgag tttaccgtgg atatggttga
    50281 tagccggaga ataaaacgtg ttaaggttaa agtgcatgat agagaagatg agcaatcgaa
    50341 ctccatttag cctgctggca atcctgtttc tttctgttat gctattcggt tgtgccgatc
    50401 ctccggttcc gaaaccaagg ggctattttc ggattgattt ccctgcaaaa agctaccggc
    50461 tcttcgattc tatttaccct tttcgcttca tgtaccctac ctattctgtg attgttccta
    50521 aggaaagcgc tgattctggt gtatggctta atgttgactt tacagggtat aaggctacac
    50581 tccacctgag ctacaaaaac gtgcatggcg atatttctaa aatgattgag gatgcccgct
    50641 ccctggccta caagcacacc gtaaaggctg atgctattga tgagcagttt ttttcaaatc
    50701 agcaggaaaa ggtttacgga gttctatacg atatacgcgg caactctgcc agctccgttc
    50761 aattctttgt aaccgactcc acccagcatt acctgcgtgg tgccctttac tttaggtgcg
    50821 ttccaaacaa ggattctctt gctccagctg ttgatttctt taagaaggat gtagttcgcc
    50881 tgcttgaaac cctttcatgg aagtagctca aatttaagta tatgccaatt attaaggaga
    50941 ttctaaacga gggtaaccga attgtggttt ggcagttcca cgacggagag ttcgagtctc
    51001 ctgcgctaca gcatgaaagc ataagcgatt ccgttgctca cccaaaacgt attcttgagc
    51061 gacaagctgt tctggctata ctaaaataca tgggatttgc caatggctac tcctacaccc
    51121 ccgaatcgcg tccttttatt cccggttcca accagcatat ctccataagc cattccggca
    51181 gtgtggcggt ggtggcggta aataaatcaa cgccggttgg ggttgacgtg gagcaactgg
    51241 acagaccatt cgatagggtt gcaaaaaaat tcctctccaa agaggagcat cttgtagtta
    51301 acgttcacga taaaagtgag ttggcgctga tttggtgcgt aaaggaggct acctacaagc
    51361 ttccatgggc taaaccttgc agcctggctg atgacatggt ggttacgctc gataggaaac
    51421 agatggaaag tggctggtgc agggtcaaag tgaggaatta tggtagcgta gccgagttaa
    51481 aagctaattt tacctttaat ggtggctatt gcctggcatg ggttaaccgg tagcattttg
    51541 gtgtatgaca aacatttaca gctcattatt cagtggcgga aaatcaaggc agatagccct
    51601 gctgctcgat cccgacaagg gaaacgaatt aagcctcagc aagactctgg agttggccaa
    51661 ccaaacccga gtttccatga ttttggttgg aggtagcctg ataagtggcg atattaacag
    51721 ttttgtgcta cgggtgaaag agcatacagc ccttcctgta attttattcc cgggtagtgc
    51781 ttttcagttt acctcccatg ctgatgccat tcttttactt tcccttattt cgggacgtaa
    51841 ccccgagctg cttataggta gccacgtgca ggtggcaatg aggattaagc agagcggaat
    51901 ggaggttata cccaccgggt acatactggt tgatggtggc gctgttacct cggtgcagta
    51961 catgagcaac actatgccta ttccatccga taagcccgat attgttgtag caacggcttt
    52021 agccggacag caacttggac taaagctaat ttaccttgag gcgggtagtg gggctaaaaa
    52081 ctccgttccg gctgagcttg taaggcaggt gcgaagccaa atttccattc ctctaatcgt
    52141 tggagggggc cttcgcagcg ccaggcaggt tgacgcggtt tttgcagccg gtgccgacat
    52201 ggtagtagtt ggcaacagcg ttgaaaacaa ccctgccata ctcgaagagt tgggctagca
    52261 atgcttactg aagggcacaa cttagggttc aaccagctct cttacagggg aacccatcga
    52321 accgttaggc caggttatcc ccagcaggct cgaaagggta ggagctatgt cataaatgtt
    52381 taccggtgac gtaataacgc gacgtttaac cttccagcca taccagatta ggggtacatg
    52441 ggtatcgtag tcgtaggccg agttgccgga ggtaatggac ccattccgtt caacccatcc
    52501 cggctccagg ttaataataa catcacccga acggcgttgg ttgtagctat tctgaaattt
    52561 caccattatc ccgtttgcaa aatttgctga ttggagggta tgagcggttg cactgttggc
    52621 cacaccggag aactcaagca taaaatcggc aaccttttgc tgcatttcgg caagatttat
    52681 gttggaatcc tctatcaacc ttcggttgag gtagatctgt ttttcgtggt agcctgttat
    52741 ccagctgccc tgtccgtata gggcattcag gtagctaccc agcagcacca tggctttttt
    52801 aggatcgaag tatccacctg gaatctttga cttttccaga aattccggag ttgacgaaac
    52861 cccttggtcg gaggtgagca ccaccagcac attctccttt ccgacctggg ttgccaggaa
    52921 ttggaggaag tgtgccagtt ctcggtcaag cctcaggtag gtatcctcca tttcaatgga
    52981 atgtgggcca aactttcggg ttatgtaccg gttggcgcta aaggtgatgc agagtaagtc
    53041 ggtgttttca tcgctaccaa ggctttcacc taagatggag gctatagcca tatccttggt
    53101 aagcgagtta ccaaatgggg tttcaagcag ctttttgtag ctggtagcag gttccgattt
    53161 tcctattatg ccgtcaatca gtagcttaat tttatccgat acccttttct ttccctcagg
    53221 agtcctggcg gtgagtgtat cggtttcttc ataagcctgt atggggttta tcgctttcca
    53281 ctccttttcg gcataaagct ttgcaagcga tttagcgtta agggtatcca cccatgcggg
    53341 taggctgtcg ctaaagtagc tgctggaaac ccatccaccc ttttcggtgt cgagccagta
    53401 aatcccactc gcgctatgtc cggccatgag tatggccgaa ccggcatcaa gagcaatgct
    53461 caccaccttc gattgggggt tgtttaggca aatttcatca cccagagtgc tggtaacaag
    53521 attttttgcc gaggccctac cgctggcgaa gctgccaccc gtagtggttg ttttttcgtc
    53581 ggcaacagca tcaaccactt cgccgttaac tctcgagaac cagctgtcgg atattatgcc
    53641 atgaattgat gggttggcgc cagtggagat tgtggcaatt cctgggtagg cttgggttaa
    53701 aaggtagctg taccttgcat ttttgcagct ggcaccatcg ttaaccagca ttttaaatcc
    53761 accatcggaa aacttatccc agtaccttgt caggtagtcg aaccgcatct ggctagctac
    53821 aatttcgaca atcaaacgtg gtttttctga tggaatttga cggttgggct gtgcccataa
    53881 ggataaccta agtcctgcag tgaacaggat taacaagcta agtctcttta aatgcatggt
    53941 ttatggaact tctaaaaaat tggtaatggt tttttcccca agttgcttgg gaatgccggt
    54001 acaaagcaac ttatttttgt tgaatctgaa ctatttttct aacaattatt gtgtcattta
    54061 ctcttaccca aaccataaag atatttccac ttgactgtgt tttaggcata ttgtcatatt
    54121 tccacgtgaa gttgtgaatg cctggcgtta gcataccgtt gaatacattg tccattctgc
    54181 agccaaggat actatacact tccagctgaa cctgggagta ttcagaagtt tcaatttcta
    54241 tgtttaccct atcggtaaag gggttaggaa atattcttaa cgattgggta ttacctataa
    54301 taggttctga agcatttggg ttaagagaga tggtgtaggt agagtcgctt gctatgctta
    54361 tatcaccttc ataggtgttg tacccgcttc gagatatgtc aacatggatg tttggtgcaa
    54421 aaggaatgcc ggtaaaggtt gctattccct ggctgttggt ttgtagttgc aggtcgttca
    54481 gggtaacggt tgcctcttcc acagggtttc cctccgaggt tacatgaaat gttacctcaa
    54541 agtatgccga aatgtccatg tcgtagctat tttggcctgt ccaaaccaca ttcaccaggc
    54601 tgtcggccag ggtgtttccc atatattgta cactcaccgg ttttgccaaa cctgtgggca
    54661 cgttgacgaa gttaaccttg ccatcgcttc cggtgtttgc cgtcatacca tcaaggttga
    54721 cggttaaacc ctcgtattca tttccattga ccagcacggt aaaggtggca ttttgcaggg
    54781 taggggagag gatgatgtcg tcaacccatg cgcaatcgct tccacctata accatttcat
    54841 cctttttgta ctcccatttg agggtgtggg ttccggaggt gatgctgaag gactcctcac
    54901 cccagctctg ttcaccgctc cattctcctt ttttggtacc atcaatgtaa aatctcaaaa
    54961 aatcgtatcc ctgttcggag gagaccttcc gggcaaaggc tacctcccca ggcccgttga
    55021 agttctgggt agctgtcatg gctgtactgc tactgtggcc gatgctggcc gagcgtaggc
    55081 tatagctgcc cgtgtaggct gtttggttgg tacggctcca gctgccgccg gtaaaggtta
    55141 gcgtggaggg tagctgcagc tcaaagcttt ctgcctcaga gggggctaga atgaaattca
    55201 gggtgttact ggagacggtc aggttcaatt cttgagttga aggtatgtag ccagctttgg
    55261 atgccctcac ctggtagtag ccttcgtata tggcagagaa gacataaatg ccctgagcat
    55321 ttgtgtaaac cggtgtgtag gaggaaccca ccagctcaac cttggcccct tcgatggggt
    55381 ttccggttaa gctgtcggta gctaatcctg aaaggttaac ctgctgagct ggctcaagca
    55441 caacatcctt cataattctg cttgaatagc tgggaacgct tacagtgtgg atttggctaa
    55501 tatagccatc cgattggtaa agaacctcat aggttcccgg agcaatcatc cggtagtagc
    55561 ttccatgctc agggtttgta accacatgcg agctatccct gtcgtggccg ggaattgtga
    55621 tggtggcatc gaggggttgt ccatccggat tggttaccgt gccgtagaaa ccggtaaggg
    55681 catgctcaag gtagccgata agggattccc tattgtagcc ccagtaggcc ggcagctcat
    55741 cggagctgag cagcttggtg ctggaaatct ccagggtaat ctcccggcag tggtgccagt
    55801 agttcatgta gtcctgccgt ccgccggtta tcacgtacca gtcacccccg ttggtaaccc
    55861 cgttgttttc atccgtaaaa tagcctgcag ggctgtgagc atggactgaa tcggcgtagg
    55921 cccggctgat ggtgtagtac cagctgttgt cggcatgagt attctcggcc gatgtccatg
    55981 tgtcccacgg gtagttggcc acctcggctc cgccgtggaa gtttgccgat aggatgaaat
    56041 ggtgctcatt ggcaaagctc atcatagcct gtgtttcggg ttgccatgag tagccgtcgg
    56101 ggtggtcgcc atcgcgggga tcggggaagt tacggttcag gtcaacgttg ttttggttgt
    56161 agcgtcgtgc accgtaaacg gtgctatctc cactgtagta ggttccatcg gggttggcgt
    56221 taggattgat gtatatggcc gtgccattca acaggttggt tatccgggag ttgctcccgt
    56281 actggctgag caggtagtcg gccagcctga gcatcagcac aaatcccgtt acctcatcac
    56341 cgtgtatggt gctggagtag agcacttcag gttcagcctc gttggcctgc acgttgccgg
    56401 atatttttag cacgtagagc ttacgtcccc ccacggtggt tccaatggag tcgagcttgc
    56461 agagctgggg gtagtcctgc tcgaactttt tcatcagctc gcggtaaacg gggtaggtgg
    56521 ggtacctgtc ccagctagcc atttggccaa tagtggttgc catgttgata acttttccgg
    56581 ggagcaaaga gggaggggtg agcagctcca ccttgtaacc caggtcggag aatcgggcaa
    56641 actcctcatc gttggcgtaa gcccataccg tatcggcctt aaccctgtca atggaaataa
    56701 tgcgggtaat tttggtattg atagtttctt tactgggttc aacaaaccgg aagtagcgtt
    56761 cgggatgctg agcgtgaagg gtagtgcata aacacgtaaa tacaattagc tgggttatgt
    56821 gtaagtactt catcaaaaag tgattttttt aagattccat attctaccaa aaacagcaag
    56881 attgccatga gacgaaatat acaaattcaa aaaaatgctt taaaacatac caactttgct
    56941 gccttgaaat tgcaggttga aaaacaacca gccgttctga aatgtagcta tactgcattt
    57001 gtttaaaggt caacattagc aggttgttat ccttttgaat gtttcaacca tacaaaaata
    57061 tgaaatatgt catgataagt ttaaaggtaa agttttattt ttgtagcgtt cacttaaagc
    57121 tgggcattag aagttaaggc ttaagttggt aggttctgct tacatgcagc acacttgttg
    57181 aattttaaac gttgaaaaat gagcactggt aaaatgtacg ataaggtctt cgccattttc
    57241 ggggtattca tggtattctt ttatatcggg ctaggggtgt tcatcatctt tcatcctctt
    57301 ctcgaccatt tggataaagc cctaaggctg atattcggca taacgttaat tatatacgga
    57361 atttaccgga taatgtattc gtatgaaaag ataaagaagg ttttttttag caaggatgat
    57421 gatgacgagt agcagcacat gcatgaaaag ctctcaatat attcttcccc cctttctgtt
    57481 agcttgttga gcctgtttcg catgcaaatg ttaagcaggt gtttttttac atgcggggtt
    57541 ggtgtatatt tgcccgctct aatgttaaaa agtattaaac gataatggta acaagttgat
    57601 agctagcagg gtattaacta ttatggactg aaatttgagg ttacctaaat actataagtt
    57661 ttcaagataa ttactaatat tgtgcgttgt taaagtattg aatagggcaa agcgaagaat
    57721 attagttgtt ggccggaaaa atttaatact atgggaaaaa tttcaacatt caaacaagtc
    57781 gtacagctgg cgattttagc tgtagccatt gtggctgtgc tacccgcatg taaaaacaag
    57841 ggagcaaagg tccctgccga aactccgacc tctggtagca taaagattga agcagatgaa
    57901 tcgttcaaac ccattgtaga tgctgaggtt agcacattca cctcgctcta taaccttgcc
    57961 catatccaaa ccgtttacaa acccgagagg gagcttattg ccgattttct caacgattcg
    58021 gtgcaggtaa tcgtttcgtc atggactcca accgaggagc aaaggaaaac cctgcttgat
    58081 gctcaaactg ttgttcgtac tacgctggtt gcctatgatg cgttggcaat tgtgctgaac
    58141 aagggaaata acgactccct tttaacctac gataatgtta aggatatttt taccggaaaa
    58201 atcaccgact ggaaccagct gaatacccgt tccaaactag gtaagattag cattatattc
    58261 gacaacatcc actcaggaaa tatccggtac ttcagggaaa agttcaacct tccacagcag
    58321 ctacccccca acttctacgc tgtaaactcg aatgcagaag ttgtaaacta cgtggctaag
    58381 agtcctggtg ctctcggaat agttagcgta aactggataa gcgataccga tgattccaca
    58441 agttttgacc ttggcagcaa ggtaaagcta gcagccattt cgcagcagta tttcgataag
    58501 gattcctact accttcccgt tcaggggtca atttaccaga aaacctaccc ctttatcagg
    58561 gaggttaaca taaactcaag ggagtcattc tacggcctag gatccggatt cataagtttt
    58621 gttgcaggtg aaaagggtca aagaatcata ctaaagtcgg gcttggtccc ggctaccatg
    58681 ccgattagaa ttgttgaagt aaaaaacgag taactaaaat taacagcaat gaacatgaga
    58741 cgtattaacc gagcattgct tcctgttgcc ttattaagct tgacctgtgg cgttggttta
    58801 caggctcaga ctcttcagga ggccatcaca ttaactaaga atgagcagta tgataaagcg
    58861 gaagccatgt ttcagcagct cattaaaaac gagccgggaa acagcaaggt atatttcttt
    58921 tatggggaaa atgtcctttt ggactacttt gccgatacaa tctccaactc gctgaacgtg
    58981 gctaccaaga aagctgccga catatttaac aatggcatca cggcaggccc caatgaaccg
    59041 ctaaactatg ttggtttagc cagggtttca ttttacaaca gggatgatgc caaggccgag
    59101 gagtttaggc taaaggccaa aagcctttta ccgccgtaca agaaggtcag caaaattcca
    59161 aatcctaagg attatgcctt tactcttgcc aagattggcg aatcctacat acgttttgga
    59221 caggttgata ccagtaaggc tatgccctac attcgacaag cgctacaaat tgattcacga
    59281 aacagtgagg tttacatcat tgccggcgat ttgtacatct tggtaaacga cggctccaag
    59341 gcaataaaga actacaacct ggctcaggac tgggacccca cttcaccaac tgccaacatg
    59401 aaaattggaa gcatttacgt gaagggccgt aacctaatgg ctgctattcc tttctacgag
    59461 caagccattg ccctcgatca gaactacgca ccggcctacc gcgagctggg ccagctatac
    59521 tccatggctg gacgattcaa cgattcaaaa aaatactttg aaaaatacct cgagctcacc
    59581 aagggtaaca ttcctgcaaa gatcaggtat gttaacgccc tcttctacgc caaggaatac
    59641 aaggaagtaa tcaaaaacgt ggaggaaatc tttgccattg atgactccag ggcctacctt
    59701 aaccgaattg caggatactc tgcttttgag gagggaaact acgagctggc caagaattac
    59761 atggataagc tttttgcctc tctcgacccc gaacgcctta ttaagaagga ctacctttac
    59821 tacgcccgaa tactttcacg caagaaccag aacttaggaa agctaactat tgaggctgac
    59881 aacatggagg ccgacttaac caaacttcaa accaagtatg cctcagctaa gcccggacca
    59941 gccaaggata aggccaaaat tgaaattgat gctttgaagg ctgaacttga ctcggtaaaa
    60001 accctggttg aaaggtcgga agctgagata accatggcgc tggaggcata cgacaaagca
    60061 gccctattcg acggtgagga ggacttgaac attacctatg aaaagggtac agtgcagtat
    60121 gccgccaaaa gatatgctga tgctgctaag acctggatgc accttattga tctaggtaga
    60181 aattctgaaa atgactacat ccaggttgga aaggcctttt accaggcaaa ggaattcgac
    60241 aaggctgact cagtgttcaa cgcaatggtg acaaaatacc ccgacaacct gcagggatac
    60301 ctttggatgg ccaacatagc ttcggctaaa gatcccgact cggagcttgg cttggccaaa
    60361 cccaaattcc agcttgtttt gcagaaagct gccgttgact ccgttaaata tgttaaggaa
    60421 atgtacgatg cgctccgcta cctcggatac gaagctctgc agaacaaacg ctacgacgag
    60481 gccaaggcat actactatcg gatgatgaac ctttcgcccg attacgttat cagagcacac
    60541 agctccctca gcaccatgta catctccatg ggtgattatg ccaacgctat tgccgagaat
    60601 aataaaattc tggccattga ccccaacaac gaggtagcca aatcgtccat acagtacatt
    60661 acacagctcc aaaagagcgc tctgcccaaa gccagtccta acgagatatc gggtgttatt
    60721 accgattctg ctggtgagcc tattccaggt gcttcagttc gcgtaaagga taccgctgcc
    60781 gaggcctgga cgaatgccca gggtgcctat aagtttgtaa tgcccgaagc ttccgaggtg
    60841 cttgtggtta gcgctaaggg ttacaaaact gttgaaatac ccgttaccaa gaagagggtt
    60901 tacaacgttt cgctcgaaaa gtaacgcagg caaaatgtta cactaatttt atacggagta
    60961 ctattgtatt aagtcagata atatttattt ttgcaatcta ttcacttaaa ccaaataaac
    61021 attactaaaa tgagcaaaaa ttcaaagtcg tctggtggtg ttttgcagtc gcttatggca
    61081 ccccttacaa ttattttagc atttatcgtt gcttatctta tctttaccag agtgctgggt
    61141 aacccggtta actttgaagg tggcaacccc aagggtcatc ccttgcaggg gaatctttta
    61201 ggtatgatgt ataaaggggg agttatcgtt cctgtcctta ttacggttat cattaccctt
    61261 ttgacctacg ttattgaaag gtacatcacc cttacaaggg taaaaggaaa atcaaggctg
    61321 aacgtgtttg tgtccaacat tcagaaaatg cttgccgaag ataaaattga ggaagcaatt
    61381 gaggcatgcg acaagcaaaa gggttcactt gcaaacgtta tgaaagctgg gctctcacgt
    61441 taccgtgcgt tggagaatga taaggagttg gaaaaagacc agaagattac ctctatgaag
    61501 caggcaattg aggaagctac cgcacttgag ctgcccgttt tgtcaagaaa catggtattc
    61561 ctttcaacca ttgcctctat ctcggtgctg atcggactta ttggaacggt tcttggtatg
    61621 atccgtgcat ttgctgcact tgcccagtcg ggtgctcctg atgctcttgc cctttcaacc
    61681 ggtatttctg aggccctggt taataccgcc ttcggtatca cgggttcaac cctggcaatc
    61741 attttcttca acatgtactc aaccatgatt gataactacg ttttcaaaat tgacgaggca
    61801 ggatttagcc ttacccagac ctttgccgca tcaatcagaa agtaagctgc aggttcattt
    61861 tgatcagaaa aagaaaggac ttttaacatg ccaaaaatta aactaggtgc caaatcgccc
    61921 cgtattgaca tgacgccgat ggtggacctg tttacgttgc tgctgacatt ttttattctc
    61981 acggcaacct ttaggccaca agaatcggca ccagttgata cacctttttc tgtatcggaa
    62041 aaacctatta tggatgctaa cgttatgacg gttcttattg cgaaggataa gcgagttttt
    62101 ttcaatattg acaacggacc cgataccatt ttaaagttca ggcccaaggt gctggccgaa
    62161 atgggcaagc gctacaatat cgagtttaac gaagccgaac ttcgtcagtt tgagaaaagc
    62221 gggacagccg tcggcgttcc gatacagaaa atgaaggaat acctaaatac caaaaactct
    62281 gacgaaaggc gtgtgctcga aacaggagta ccaatagact ccacggacaa ccagcttgcc
    62341 ctgtggatcc tatttgcccg ccaggtgaac cctgccgtgc aaaccatgat taagggtgac
    62401 gccgatcttg aatttcccgt cgtaagggaa atactgtcta tactgcagga taagaacgtg
    62461 aacaggtaca gccttgttac aaatcttagg gctgtagagg tgaaggaaga tgaaatagct
    62521 gagtgagatg gcagaagttc aagtacaaga aaataccaag ggcgggaaaa agaaacccaa
    62581 gaagggtgat gcgcatgtag atatgacgcc catggtggac ctaatgagcc tgctcattac
    62641 gttcttcatg ctaactgctg ctttcagcaa accaaaggtt atggaaatcg ttctccccga
    62701 gaaaattaaa aagggcgaga aggttgaacc accaaaaatt gccgaaagcc gtactctcaa
    62761 cgtaatcctg ggaccaaacg ataaggtgta ctggtacccc ggtaaggccg acccagaggt
    62821 taccgtgctt caggaaaccg actttagcgc tacgggcatt cgtaaggtgc tactcgatcg
    62881 caacagggca ctattccgca aaattgacga ttttcagaaa aaggtcataa gcggtgaaat
    62941 cgagattagc caggattctc tacgtagtgc cattagccag ctaaaaaagg acgacgatac
    63001 gggcccaatt gttctcatca aggcatataa aacatcaaag tacaaaaact ttgtggatat
    63061 tattgacgag atgtccattg ttggaattgc ccgatacacc gtaaccgata tcaactgggt
    63121 tgaagaaaag ctggttgaaa aggctcttgg aatagcccct gtggctacaa cacaaacagc
    63181 taaataagga ggttgatatg acaagaacat actatcccag ctttgacgat attgtatttg
    63241 ccaacaggaa taaggagtat ggcgcttatg ttttacggaa gaagtacacc cgaacgatgg
    63301 ccctgtctat aattctgggt gttcttatca tcgttatggc cgccattatc ccttttatcc
    63361 gggcaaagag cattgccgct gctaaggcta gagatgccaa cgaggttatc gctcaaatgg
    63421 ccaacgatat tcagcttgat caagctgcac caccaccacc cccaccaccc ccaccaccaa
    63481 ccgaacagca aaccgtggta aaatacgttg ctcctgttgt ggttgacacc gttaagcccg
    63541 aagagcaaag taagcttatg attgccgagg aggcagttga aacggtagtt gacaaggaag
    63601 ttgttgaggt tgtggaggtt gtggaggcac ccaaggagga ggttgagtac gaggctccgg
    63661 ctgaggtatt cgtagctgtt gaggaaatgc catccttccc gggcggggat gccgagctat
    63721 tcaagtttan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    63781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng ctgttgagga
    63841 aatgccatcc ttcccgggcg gggatgccga gctattcaag tttatctacg aaaacgttaa
    63901 gtatcccgac attgccatgg aaaataacat ccagggtaag gtaattgtca ggttctgcgt
    63961 aacctacaag ggtactgttg accatgtgtc ggttctccgt ggcgttgacc caaccctcga
    64021 cgaggaggcc attcgggtta taaagatgct acccaagtgg aaacccggta agcagggcgg
    64081 taagccggtt aatgtttggt acaccgttcc tatcagcttc cagctgaagt aggagtaata
    64141 tagttttttg tgaaaaaggc gcttcaaccg atgcgccttt ttcatttacc ccttgccatt
    64201 ttttttcgtg aatatttttg aaaacatttc aacaggtgca tacgtaaaaa atgaacctaa
    64261 aaaacatcgg gagtatggct attcgaatta ccgagctatt tggggcaaag gccggagatg
    64321 attggggcag gcactacatg ggcatcatca aacattgcgt gctacctaat ggacagcagg
    64381 gtgtattcgg ccgaattgat attaaggata gcgagcataa cgaggtagct tgcgctagtg
    64441 ccaatagccg tgaacagctg ggtgtaaagc tcgatttgct tgttttgatg gtgctggatt
    64501 atgggatact cgatttgaag gcgaatggca tgaatagcga tacctttggc accaacatga
    64561 ccattgggct aagctgagag ctggttgtga tttaccccac gattttgtac ttttaaaagg
    64621 tttttttgtt aaaaagcggc ggttgaaatc cgtgaatgtt cgctattttt cttttcctgt
    64681 caacaaaagc aatttactgt tgtttacatt aaggttgaag tattgcccag gctctacggc
    64741 caggcagtta accctttcta tttctttgga ttaagacgac agggtaatac atttaagcgg
    64801 ttgggataag ggggaaagga gttttttccc ctctttgttt taaaaaatat atttatccta
    64861 cttcccatcc ctcctaaaag gctgcaccag ctacttgaac ttagatgaca aaaagaccta
    64921 tcaatattat ttcaatagca ttggtgctta tttttatagc gccaatgtca attaagcttg
    64981 ttgacggact tttccatcac catgaccatt ttcattgcac cgcaacaaac gagaagcatt
    65041 ttcatgtata tcacgaaaaa tgtcccatac cgggttttga gctatctctt ttttcggctg
    65101 aaaagcattt acaaatctct cagaaacaca attactgtgt aaaaccaaat gacgattaca
    65161 actttgttta ttgtagcaat aattcagagt actcattttt actacgtgca ccaccatgtt
    65221 tacaggtgtg aaatgtacat ctgttaaggt ataaaagttt gtttataagc taaaacgtaa
    65281 taaaatgaga agaatactta tcgttgttgc tatgatattg tcgtatcata tattgaacgc
    65341 ccaaaacaaa ataacaggga aaatttcaga tcaagctaat ttaccgttga ttggtgtaag
    65401 cgtttttgtt cccgatatga gtaagggtac aacatcggga actaacggat cttatgagct
    65461 gtcaggttta ccgaatggta aaattaaaat tcaattctcc ttgttgggct atgccggtag
    65521 aattgagacc gttgtgttat ctggagaagg gttggagttg aacatagtgc taaaccaaac
    65581 agctttagaa ttggaggaaa tagttgtttc cggtgggtat agctcgaccc aacacgaaaa
    65641 cgcagtaaaa attgatgttc tcaaactcga cccacgaaca ggtacaacta cgccaaattt
    65701 tacggaaaca ctcacaaaag ttcccggcgt tgatatgatt tctaaaggaa gtggtgtatc
    65761 gaaaccggtt atcagaggac tctcgatgaa tgatatttta gttctgaata atggggtgag
    65821 atttgagaac taccagtatt ccagccatca tccgcttggg atagacgagt ttggagttga
    65881 agatgttgaa attattaaag gccctgcttc attgctttat ggctccgatg caattggtgg
    65941 tgttatcaac ttcataaaag aaaaacccgc acccataggt agcattactg gcgattacaa
    66001 catgcaactt ttttcaaaca cgctgggtgc aactaataat tttggtataa aaggagcttc
    66061 aaataagttt tttggagggt taagagttgg tcaaaaaaca aactccgatt ttcttcaagg
    66121 aggaggtgct tttgtcccaa attcacgttt caacgaaatg tcagctaagt taaatgcagg
    66181 atttactgat aaagtcggca cttttaaatt gttttacgac ttcaacaatc aaaaactagg
    66241 actggttgaa gacgaggcaa ttgaagaaat ttcccagagg ggtagaaaaa acgaaatttt
    66301 ctaccaggaa ttaaacaccc atttgctctc ttcgcagaac aagctgtatt tgggtaaatt
    66361 caagctggat ataaattcag cgtttcagcg tacagagtta acccattttg gtgatgctgg
    66421 cgtttatgaa atacaaatga agcttgcaac atttacgtat gatgccaagc ttcatttgcc
    66481 atcaagggtg aactccgaat acataatcgg gttgcagggg tttaaccaaa caaatacaaa
    66541 tctcaacaac agggaaacca agctattacc tgatgctaca acaagtaatt attctgcttt
    66601 cggcttgttg cagtacactt tcttcagaaa attaaaactg caaacaggca ttcgttacga
    66661 ttataagacc atagccacta agggtattgg tgtggctacc gattcaatga cctatcgtgc
    66721 cccattagac aagtcatacg ggagttttag cgggtcgctt ggtgccacat acaaagtaac
    66781 ggaagagcta ttatttagag caaacattgc tgctgcatac cgcactccca acatagcaga
    66841 gcttacttca aacggaccac acgagctgag attcgaaatt ggcgatagaa acttagaccc
    66901 tgaaaattca tacgagacag atgtaagcct acattaccat aaggataact tcatcgttga
    66961 tgtggcaggt ttttacaaca ttgtcaatag ctacatattt atttcaccaa caggcgacac
    67021 aactgcctcg gggatatata tttttagcta caagcaggca aattccatat tgtttggagg
    67081 cgaagcaggt ttgcatgttc atcccaaatc gttaaaatgg ctgcactttg aaaccacgtt
    67141 ttcatccgta attggcaagc aacggaatga cctctacctg ccatttgtgc cagctaataa
    67201 attacggttt gaattacggg gtgaaaagga aaaattagcg cttattaata atgcatttgc
    67261 ctctataagc ataaacaccg catttaagca aaacaacgct gcgcctgacg aaacgcctac
    67321 tgctggatat acgcttattg atttaagcat tggtggccaa attaaagtgc agaatcagct
    67381 tatttcattt agcataagcg caaacaacct aacggacaag aaatacattg accatctttc
    67441 aacactgaaa gaagtggatt tatacaatcc cggtcggaat atttcgttga gcattcatgt
    67501 tccattcgag ttaaaagcaa gtagtgagaa ttaacagcta ctaaaaaggg gctatgactc
    67561 tccccatttt taggaacgct tatagccatt tcctggctga attttagctt taaggcctga
    67621 tgctgatggg gtgttaataa cccgaaatag ctcgcagggc tactcgggct gttggcaccc
    67681 cttcctgctc ctggtagctc cattcttcct ttttcccatt tttttatgcc ggcttaccgt
    67741 atagcacggc tgtggttttc ctgtggggca ccgaccctaa tggcgggcgt aaatgtattc
    67801 cttgttgata tcgtggcacc accgggttta gcttttttca ccataactag tggtgccttt
    67861 tttcaattcc actatggttc ggttaaaagg gggtaacttt aacgaaataa ggaaagggga
    67921 gacaaatgag ctgtttgtaa tagttaagaa tgggttgaca gaggttgccg ttattcggat
    67981 aaacaaggaa aacggatgct acattgttga aaaggcaagt gtgatgcgga aagcatatgt
    68041 aaacagaaaa gagttgctgt ggcaacggga gcgaacccta ttaacctgaa ctgccctcag
    68101 gtttctttga cctgctatgc tgcttatttg ggcaggcagc gataacagaa cgcttagaag
    68161 cacacaacaa ctcctaaata caaaggtaaa ccattttaag gttaaagtca aaaatttcaa
    68221 ttccactatg gttcggttaa aag
//
LOCUS       DAOSHM010000006        67395 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_333_length_67395_cov_22.227992, whole genome shotgun sequence.
ACCESSION   DAOSHM010000006 DAOSHM010000000
VERSION     DAOSHM010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 67395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 67395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67395
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_333_length_67395_cov_22.227992"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(6..884)
                     /locus_tag="PK839_01805"
     CDS             complement(6..884)
                     /locus_tag="PK839_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009129200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HOG71500.1"
                     /translation="MTGKRLFKEIRSYSIIVLGLLLYALSWTAFLLPHKLTGGGVSGI
                     GALVFYATGIPMGYTYFVINIGLILLSIKILGRGFGVKTIFGVTVGAVLLSVLQVVIK
                     QPVVDDKFMSVIIGGAIAGVGLGIVFTQGGSTGGTDIIAMIINKYRNISPGRVILYCD
                     VIIIGSSYFALLDLDPAGRIETIVYGYVAMAITAYAIDATLSGSKQSVQIFVFSKKYQ
                     EIADRITGDLHRGVTVVDGVGWFSKEPQKVLITLVRKYESNDVYKIIKEIDPEAFLSV
                     STVMGVYGRGFDVIKH"
     gene            complement(902..1306)
                     /locus_tag="PK839_01810"
     CDS             complement(902..1306)
                     /locus_tag="PK839_01810"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HOG71501.1"
                     /translation="MEKSENKTKSFRDLIVWQKAHALVLNIFQQVKTFPEDQQQMVGD
                     YLCESAMAIATNIVGGYKKKDRDEKLLYLSAAQDALEECRYYIILATDLSLLDTFNAE
                     DLENAIGEASFLLNSYVKSIQRRKEDDERVEI"
     gene            complement(1406..4189)
                     /gene="leuS"
                     /locus_tag="PK839_01815"
     CDS             complement(1406..4189)
                     /gene="leuS"
                     /locus_tag="PK839_01815"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005639621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HOG71502.1"
                     /translation="MDYNFKAIEEKWQALWQQRNTYRVDVNPNRPKFYVLDMFPYPSG
                     AGLHVGHPLGYIASDIYSRYKRLQGFNVLHPMGYDAFGLPAEQYAIQTGQHPAITTEN
                     NIQRYRSQLDKIGFSYDWSREFRTCEPSYYKWTQWAFIQMFKHWYNNKTQKAEPIDEL
                     VAEFERNGNQHVDAACSETKIFTASDWRNMDGRQQQELLLSYRLAYLADVMVNWCPAL
                     GTVLANDEVKEGVSIRGGHPVEQRKMRQWCLRISAYAERLLSDLEQLDWTDSLKEIQR
                     NWIGRSEGAEVLFSVADSRQQIKIFTTRPDTIYGATFMVLAPESELLDGLTKPEYADN
                     VEAYCNEVKRKTERERVAEAKKITGQFTGSYAVNPFTNQPIPIWVSDYVLAGYGTGAI
                     MAVPAHDSRDFAFARTFNLPIIQTVVRKGEQPTNPETWEESYDAKDGIVVNSPLIDGL
                     EVKQAITRICEIIEQRGLGKSKVNYRLRDAIFSRQRYWGEPFPIYYKDGLPYPLPEEK
                     LPLELPDVDAFLPTEKGEPPLGRAKNWHTPEGYPYELSTMPGFAGSSAYYLRFMDPHN
                     DSQLVSKEANTYWENVDLYIGGTEHAVGHLIYSRFWNKFLYDLGYVCKSEPFKKLINQ
                     GMIQGRSSFVYRIKGTNTFVSLNLKGSYEVTPIHVDVNIVNNDILDVEAFKKWRPEFS
                     NAEFILEDGKYICGWAVEKMSKSMWNVVNPDTIVEKYGADTLRMYEMFLGPLEQSKPW
                     DTNGIDGVHKFLKKFWRLFHNPANQLEISDDNPTPQELKVLHQTIKKVSDDIERFSFN
                     TGVSAFMICVNELTDLKCNKRQVLEPLLILISPYTPHIAEELWSQMGYTGSICDASWP
                     LFNEELVRESTFTCPISFNGKMRFTLDIPLNLPVAEVEKLVLADSNTQRQLDGKTPKK
                     VVVVPHKIVNIVV"
     gene            complement(4450..7110)
                     /locus_tag="PK839_01820"
     CDS             complement(4450..7110)
                     /locus_tag="PK839_01820"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HOG71503.1"
                     /translation="MRINKFLLVSVVPFLMLTSCQNWFNRHLIPGKTQREEVHQRFSE
                     RLKLVEAYDTSIQRIVSYDLPLKTREGFEFLYAYMPLSDLAMHSSSYVLEQVQLALEA
                     KDHFKWGRKVPSDIFLHFVLPYRVNNEYTDSARKVFFAELKPRLSGLSAYEAALEVNH
                     WCHEKVTYQPTDERTSGPLTTVRTAFGRCGEESTFTVAAYRSVGIPARQVYTPRWAHT
                     DDNHAWVEVWVDGSWHFLGACEPEPELDMGWFAAPAKRAMMIRTFVFGKYNGQEEKLT
                     ESDCFAEINLLPRYAPSKNLSVTVVDEANQPVEGARVEFQIYNYAEFYPIASKVTDKN
                     GLCSITTGLGDMMVYAHRDGKTAFVKVDRSLNEMELKLSSTTSAEATDLLLVPPDEIS
                     VTSAADDKVAANSRRLAVEDSLRGCYIATFIDSLSAVKFAASMGINAEKAWHFLSKSR
                     GNWPEIQNFITNMEPGETPVGMALLGILAEKDLHDITAATLRSHLKTVDSFPSLLDSR
                     SFNLFDRYILSPRVGREFITPWREFLQGYFTFDQIGFFRNDPTNLVKWISENIKIDSV
                     ENYYKVPISPEGVVRLGIADAYSRDILFVAACRSFGIPARLEPSTKRPQYLMRGSWHD
                     VDFGEREVEASGWGTLTLSATSGMEIASPSYYKHYTIARFENNRFVTLDFENDAQLST
                     LPFSLSLREGLYRLVCGTRDQNGAVSCRVKYFSIYPDEKTLVTLEFPSSKGKTVVLGN
                     LGDPLIPDIEGSYIKNLGEIGGGGSTVVAIIDPMKEPTRHLVNDLAAIKTQLDKWGGA
                     VVLAIAKDKLPSGFNAQKLLEGVPLPIGVTIGYDSQQSLANALAIACGKPLSTDYPVV
                     AVVNGKGEITFFSEGYSIGLGEKILKEVGE"
     gene            complement(7574..10009)
                     /locus_tag="PK839_01825"
     CDS             complement(7574..10009)
                     /locus_tag="PK839_01825"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HOG71504.1"
                     /translation="MSQVAKWLFRILAVAVFLLIAAAVVLPLVFKPQLKQLAKTEINK
                     NINATVEFDDFQVSLLRGFPNLFVGLKGVSIVGVERFAGDTLVSFGELGVRVNLWSII
                     DMEEIEVRSVYLSDAKVFARVTADGSKNWSIVKTSGLPQTEEEDTAAAKPTNIRIALT
                     RFKINNATFFYADDSLGMETTLRGVNLLISGNMVKRQTEIRLKSSADELDLRVKGIRY
                     IRDAEVRFNANMGVNLDNGTLSFRENELRINQLTLLFQGMMRMHKGSIGMDVSFSAPS
                     TDFKDLLSLIPAFYLKRYEDIQTSGQLKLYGSVNGVYNDSILPSASIEIAVANAMLKY
                     PKLPKSANGINFDAKVWFDGSNPDKSTVDVNRLQFSLGGNPFDAYLHVATPVSDMQVD
                     GKVVGKVNFATLADVIPLDSISVTGLLETNLKFGGRISHLKEKEYDKFNAEGSLNISN
                     FTLTGCDSTRQVKVGELIVNFSPKFVELQNLKSSLGSSDMQLSGRLENFIPFVFRNEA
                     LQGQLNLTSTLLDANELLSGFRVQAKQPVDSVVTHGVIKIPRNFNFTLSASVQHLLYG
                     RLDISNLKGNVEADSGIVNLYNLTMNMLGGDVALDGIYNPIDVSLPRVNLDLSIKDVD
                     IPLAFKSLTSLQRIVPTFKNLSGKLSGSISIASMLDSSMMPVPSSIVASGKLQSKSIG
                     FKESAVFGRLADVFKKEEFRSPYMSNVNVNFKVNESRIAIEPFSTSVGGAKMSVGGDM
                     GFDQSLNFKAKLAVPTELFDTAADALSKFSTKTNVRVSNLELTLKISGTSYKPLVKVD
                     WGDFTTNIDIPGL"
     gene            10374..11612
                     /locus_tag="PK839_01830"
     CDS             10374..11612
                     /locus_tag="PK839_01830"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOG71505.1"
                     /translation="MMKKIYLISFAAVAWLSACTTGEHKLATGPWLGVIQLDSTGGKL
                     DVPFNFELKNDSGTYSMTIRNADELINVDEVELVGDTLRARLPVFTSELVVYCGDSLK
                     GFYYPKGVQAGTRYKFYALAGQIDRFPWYSEPPAFNITGRWWFIENPGSPDSTVMVAE
                     LKQDGSRVTGTILSTTGDYRYFEGKVSGNRFYFSKNDGAQTIIVKGEISDENTMVNGV
                     ISGSPKWVSPWRAAKDPQAKLPEADKLVRVKKGFDTFEFAGKDISGNMVKSSDEKFRG
                     KVLAVLAGGSWCPNCLDEGRYYKQLYEKYRDQGFEVVSLCFEDKTFKESQPKIERFAK
                     SIGAAYTFLYVAPRGREQRDSVLYAIEGQMAYPTSMILDRKGKIRRVETGFSGPGTGE
                     HFVTFAKETEELVKLLLEER"
     gene            complement(11617..12294)
                     /locus_tag="PK839_01835"
     CDS             complement(11617..12294)
                     /locus_tag="PK839_01835"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOG71506.1"
                     /translation="MAESSSTSFSIRNIRNIVFDLGGVLLDIDYSKAVMAFKKLGVAD
                     DDHLLSAGSMGIFNRFDCGQISPSAFHAEMCALVGMPLSYEQVVGAWNAMLLDWDVAR
                     LELVEALRQRYRVYLLSNTNSIHYSSYNQKLEAIAGGRSLSSYFDKAYYSFEVGLRKP
                     QREIFNLVVESSGLIPAETLFVDDTEENIMMASEVGMATRWIRPGEAALLSSILAGFL
                     SEPVGRS"
     gene            12380..12453
                     /locus_tag="PK839_01840"
     tRNA            12380..12453
                     /locus_tag="PK839_01840"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12414..12416,aa:Ile,seq:cat)
     gene            complement(12596..14866)
                     /gene="rnr"
                     /locus_tag="PK839_01845"
     CDS             complement(12596..14866)
                     /gene="rnr"
                     /locus_tag="PK839_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HOG71507.1"
                     /translation="MSKKGEKPSGRTPQKRGVTKRDLREGLMDVFIKNPNQSFNYKQL
                     STRLGVNNDNTRRMITEVLYELVGEERLVELSRGKFRLQEQTSLATGILVMSKDGSGE
                     VTTDDNREIFIPDFRLNHALHGDRVEISIYRHRRMGLLEGEVIRVIERAKRNFVGTIS
                     LLRNHAFVAPDNKLMPYDIFIPHVELRDIKNGQKVIVQITDWPPREKNPNGKIVEILG
                     NPGVHEVEMHAILAEFDLPYRFPEELEKLAEKIDDRISNADYASRRDFRDVPTFTIDP
                     FDAKDFDDALSVQILPSGNIEVGVHIADVTHFVKPNTPIDNEAIERGTSVYLVDRCVP
                     MLPEKLSNYICSLRPNEEKLCFSAVFELNDNAEVLSEWFGRTVIISKRRFTYEEAQAI
                     IETGEGDLHEQILLLHKLAQKLRRERFKNGAIAFERMEVKFELDAKGKPLGVYFKENK
                     ESNQLIEEFMLLANRKVAELVGKQKPGKERFPFVYRIHDKPNQEKLDNFRTFITRFGY
                     KLSGESSRQLGKSLNSLMDEVRGKPEQNLIETLALRSMAKARYSANNIGHYGLAFPFY
                     THFTSPIRRYPDMMVHRLLAHYLSNGKPKDKEQIELLCKHASEMEIRATEAERASIKY
                     KQVEFMQDKVGQVFMGVISGVTNFGIFVELSDSQCEGLVAMRDLDDDYYQFDEETYAL
                     VGKRTGRRFQLGDPVKVEVWRTNLAKKQLDFRLVDGEVHPGNSQLQVNRPQRAKKGSG
                     SKTHSMSRSTSKRRKK"
     gene            complement(15158..>15405)
                     /locus_tag="PK839_01850"
     CDS             complement(15158..>15405)
                     /locus_tag="PK839_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG71508.1"
                     /translation="TETQDLGWIQFNSDGTGIDSEDYTFTWTLKGDKLAINQDGEEVT
                     LTLTTKDGGKMVGYFQETFTEDEGDMTVKVIIEFAKV"
     assembly_gap    15406..15505
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<15506..15723)
                     /locus_tag="PK839_01855"
     CDS             complement(<15506..15723)
                     /locus_tag="PK839_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG71509.1"
                     /translation="MRKLRLFAIVAAIALMFTACSDDDDFSVEGKWNIVKETTNVYSG
                     TSATGTPVYTYTETQDLGWIQFNSDGTG"
     gene            16257..16664
                     /locus_tag="PK839_01860"
     CDS             16257..16664
                     /locus_tag="PK839_01860"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SiaC family regulatory phosphoprotein"
                     /protein_id="HOG71510.1"
                     /translation="MISKEGLKGLVDLHIQATTETPEVICSKSTGKISMVGNLIPSDP
                     ESFFAPITRWFLLYSTEFEPKQITVDLYLTFINGCSEHHLVSFLKKVEELYSRGVEVR
                     INCHYESDDFDMRDWGRDLRLVLKIPVDMVEIN"
     gene            complement(16689..18191)
                     /gene="hutH"
                     /locus_tag="PK839_01865"
     CDS             complement(16689..18191)
                     /gene="hutH"
                     /locus_tag="PK839_01865"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016278305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HOG71511.1"
                     /translation="MTRIHRISPQPLTWKVLEDITTGDFRLSLSDESKVLIEKCRRYL
                     DVKMEESSVPIYGITTGFGSLCNKTISRNELSQLQKNLVMSHACSTGAEVSSEIVRLM
                     LFLKAHALSLGNSGVQVATVERIIDFYNNDVLPVVYDRGSLGASGDLAPLANLFLPLL
                     GMGDVRYKGKVVEALDVLKEFGWQPIELKSKEGLALLNGTQFMSAHGVSAMLKAFRLI
                     NLSDLIGAISLEAFDGRVEPFHENIQQARPHRGQVETARHIRAILEGSELINRPKKHV
                     QDPYSFRCIPQVHGASRDAIAYVASVILTEVNSVTDNPTIWPDEDLVISGGNFHGQPL
                     ALAYDFLAIALAELGSISERRTAQLILGLRGLPEFLVANPGLNSGLMIPQYAAAAMVS
                     QNKQLCTPASVDSIVSSNGQEDHVSMGANAATKLLRVVDNVERILAIELLNAAQAMEF
                     RRPAKSSPAIESILASYRRQVKFFGEDRIMFKEIDKTLEFIRNIKPGEWL"
     gene            complement(18196..19572)
                     /gene="hisS"
                     /locus_tag="PK839_01870"
     CDS             complement(18196..19572)
                     /gene="hisS"
                     /locus_tag="PK839_01870"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007214868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HOG71512.1"
                     /translation="MASSKPSIPKGTRDFNPEQMVRRNYIFDTIRSVFSIYGYQPIET
                     PAMENLSTLLGKYGEEGDKLLFKILNSGDFLERVDPSTARPGNSNAVSLAICEKGLRY
                     DLTVPFARFVVQYQNDIVFPFKRYQIQPVWRADRPQKGRYREFYQCDVDVIGSTSLLN
                     ELELVLIINEVFQRLGVRVVVKLNNRKILSGIAEVIGEAERLTDITVAIDKIDKIGID
                     GVNSELRERGVSEVAIEKLQPVILLKGNNTEKLGLLKNTLAPSEVGMRGIGEMEELLE
                     LIRQSGELLSEVELDLSLARGLNYYTGTIFEVKAADVEIGSICGGGRYDDLAGIFGMK
                     NLSGVGVSFGADRIFDVMLQLNLFPDSLLSSTQVMFTNFGKSEAAYCLKALRQLREQG
                     VSAEIYPDPAKMKKQLDYANRRRIKLVVIAGESEMQQNQLSVKNMETGEQATISAEDV
                     VSYIKENI"
     gene            19966..20697
                     /locus_tag="PK839_01875"
     CDS             19966..20697
                     /locus_tag="PK839_01875"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HOG71513.1"
                     /translation="MNGQLKSLLRKALNRLHLDLTKNLRYDRLSKRIISLHLKPDSTC
                     IDVGAHRGEILDLFLQFAPKGHHLAFEPIPSLYNALEKKYSGRCRVYPFVLGDRIGKT
                     PFQLVRNAPAYSGILKRSYSIRHPEVEEIEVEMATLDSVVNEEENIDFMKIDVEGAEL
                     QVLLGARSTLCRCRPNIIFECGLGASDYYGTNPDDLYDFLTGIVGLKISTLKGWLTDS
                     GNPLTRDEFRRHFERNSEYYFFAHP"
     gene            20914..21399
                     /locus_tag="PK839_01880"
     CDS             20914..21399
                     /locus_tag="PK839_01880"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOG71514.1"
                     /translation="MTVEDFNSCVDLYSDGIYRFALKMLGDTMLAEDNVQDSFERLWV
                     KRGEIDRSKAKSYLFTTSYHASVDAIRRDRHKQELENAQPVLEIGYQPNFDVKEIINH
                     AVDQLPTVQKSVLLLRDYEGYSYGEIAGITGLNESQVKVYIYRARVFLKEKLVSIENL
                     I"
     gene            21405..22424
                     /locus_tag="PK839_01885"
     CDS             21405..22424
                     /locus_tag="PK839_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG71515.1"
                     /translation="MSINLNTYEEYLLDYIDGKLCPEVEAELVKFLRLHPDIASELDG
                     LNQLTIPKPNLSFSGKQRLRRTVEQDLFSCNEMDYLCIAELEGDISEVEKARLEELKG
                     QHPEVERFRNLYAHTLLLSENSHTFPNKASLKRHSIISKRRIMLTAAAAITAAIVGLH
                     TIHKLSLPDDLQPQNQMAKSAIIESQPRENRPTSTTTYPQTQPKPESRVAVSLAYKTS
                     TSTISMDNEELQPMRTNVAELKQIASKEPIIEPIASITPKPLPITGKVQGVAFREKEL
                     APNVAPSTAKVYTLADIAGLSLKRLAGLVGISYNVKTDSTNTSKHIEVDSRLIAISTI
                     IRKNN"
     gene            22497..23579
                     /locus_tag="PK839_01890"
     CDS             22497..23579
                     /locus_tag="PK839_01890"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOG71516.1"
                     /translation="MKQLVILASSLLSFFTLTAQNEKETMLRSLEIADTLKVSDTLST
                     NHRKPNMVVNVEKTIKVADGDTVEETTSDTLVVDSNTGDNNVAYQGDTIIVDRNQGNT
                     KSDSTIIRLGEKTIVIVEGKDKTTVEIPEGLNKDFRNSFRRSNRLKFKGHWAGIEMGV
                     NGFMDADHSTSMSNELEWLDLKQSRSWNVNLNFLQYSMGFGTSWIGLVTGMGLEFNDY
                     HFNNPLTLKVVNGVTVADSSYLLAGYKVDKTKLSTVNLTLPLLVEFQIPTSESDRHKI
                     YISAGVIGGLRIGTHTKVVFDDGDRHKDKGRSDFNIATFRYGVTARAGYRSLRLFANY
                     YPTQLFEKNKGPEIYPFAVGLVLIPF"
     gene            complement(23931..25265)
                     /locus_tag="PK839_01895"
     CDS             complement(23931..25265)
                     /locus_tag="PK839_01895"
                     /inference="COORDINATES: protein motif:HMM:NF027964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HOG71517.1"
                     /translation="MSRILILGAGLSSTNLIEYLLGNAPAYGWTIRIGDLSLEAAAGK
                     LGGNPNGQAFKFDIFNAEQLSAEVAWADVAISMLPAFMHPVVAKECVRQGKHLLTASY
                     VSDAMKSLHDEARQKGVALMNELGVDPGIDHMSAMRMIDGIRNKGGKITGFISNTGGL
                     IAPECDNNPWNYKFTWNPRNVVLAGQGVTHFLENGEHKYIPYHRLYSNIRTYNIPPYG
                     EFEMYPNRDSLKYREIYGINDIQTIIRGTLRRSGYSKAWDVFVQLGMTDDTYKMEGSE
                     MLTKREFLNSFLPYNPTETVEEKLQRVIPAAQDKIIFNKLKWLGIFDNELIGMANASP
                     AQLLQRIMEEKLGLEPNDKDILIMQHIFDYELDGKKFQKKSTLTLVGEDTVHTAMAKT
                     VGTPLAIAAKMLMTGQVNDRGVVMPIKPHLYNPILNELEEYGVKFVEEERQL"
     gene            25635..25886
                     /locus_tag="PK839_01900"
     CDS             25635..25886
                     /locus_tag="PK839_01900"
                     /inference="COORDINATES: protein motif:HMM:NF014470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HOG71518.1"
                     /translation="MLTLSIGFGELVVILLVTLLVVGPREVAKVARKLGKLYRRMDNA
                     MSDLRSYVENPSSPGDNSDLVDTSEKRNDSDNANKTPAG"
     gene            25954..26607
                     /locus_tag="PK839_01905"
     CDS             25954..26607
                     /locus_tag="PK839_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG71519.1"
                     /translation="MIKVENITKSYGALMVLKGITLEIPEKKVVAIVGPSGAGKTTLL
                     QIMGTLGRADSGQVLINGENVAQLNDRQLAKFRNTHIGFVFQFHHLLPEFTALENVCM
                     PALIAGKGMATATKKSTELLKMLGLSDRLDHKPSELSGGEQQRVAVARALVNNPTVIL
                     ADEPSGNLDSRNKEELHKLFFMLRDQLGQTFVVVTHDKDLAQMADLQVGMNDGLLCP"
     gene            26604..27383
                     /locus_tag="PK839_01910"
     CDS             26604..27383
                     /locus_tag="PK839_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018335878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02757 family protein"
                     /protein_id="HOG71520.1"
                     /translation="MKLPQNRAELTQLLNNRYHRYAGASFLSDDPILIPHRFTKTEDI
                     EISALLTATIAWGTRKSIIGSMNRLMAIMDNSPFEFVINFSENDEKRLNGFAHRTFNS
                     SDCIAFIRSLQRIYADGGGLKAIFTNGFRQTRTAKQALEAFRLRLIQENFSGHAMKHV
                     PNVESGSAAKRLNMFLRWMVRPPKEGIDFGLWSEMPTSALLIPLDVHSGRVARALGLL
                     TRKQNDFKAVEELTESLRGFDADDPVKYDFALFGMGVHGEI"
     gene            27403..28116
                     /locus_tag="PK839_01915"
     CDS             27403..28116
                     /locus_tag="PK839_01915"
                     /inference="COORDINATES: protein motif:HMM:NF017755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOG71521.1"
                     /translation="MANSYFRFKQFKVFQEKSAMRVNTDGVLLGAWADVSGVQRILDV
                     GTGTGVVALMVAQRNPTAMVDAVEIDAPSAEEAAGNAARSPWANRIRVVCTSFQDFAS
                     SAEFRYDLIVSNPPYFNRSLKPSDGRRTITRHTDTLPTPALLEGATKLMLPGGRFCGI
                     FPYAEGNVFIAQASHYGLFCSKKLNILSKPGGRTLRMLVQLEAERKATVESSLCIHTV
                     DGSFSKEYRNLTSEFYMDF"
     gene            complement(28567..28830)
                     /locus_tag="PK839_01920"
     CDS             complement(28567..28830)
                     /locus_tag="PK839_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018712249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HOG71522.1"
                     /translation="MTRITFSRNSWEEYKSWQVDDRLILKKINELIKDIERHPYEGLG
                     KPEPLRFDLAGFWSRRIDREHRLVYRVIDQEILIYSCRYHYDK"
     gene            complement(28830..29081)
                     /locus_tag="PK839_01925"
     CDS             complement(28830..29081)
                     /locus_tag="PK839_01925"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HOG71523.1"
                     /translation="MLAANFTEFRVGLKKFLDEVEMNNETLIIKRGAGMGAVMMSLEE
                     YNSIMETLHLLSSKANAERLFESINQMNERKVVKGKLLE"
     gene            complement(29548..30246)
                     /locus_tag="PK839_01930"
     CDS             complement(29548..30246)
                     /locus_tag="PK839_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG71524.1"
                     /translation="MMTIYIKCVFKNSYYSLRRIKVKTSSGGTYYVGHEEVITINSGN
                     DEWLDFRLDYHKHRFTIDRNTNDQKLYLVVMLEFRDKFPFSHLDVMFRNCMRVKAVDK
                     YQFDNFENTIAITKFTSKEYSFIDYFTCTLCLLIFLGYTVMSVIYEGIDDRNFIFIIG
                     IAGVFSSLRLIILRKSISSGSFDVVIRTSSFINLILLIFLDVPTLAKVLFLGISVLLF
                     LIDIYNKHFQKVDE"
     gene            30981..32441
                     /locus_tag="PK839_01935"
     CDS             30981..32441
                     /locus_tag="PK839_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOG71525.1"
                     /translation="MEKSSFNRDVKREKRTNSQEEPTRVYRPRTLTSRKATPETENEN
                     STPSSEGEPKKFRDDYDNSGQHSEQQNEEPTQQRRKLKPIAAPAGDFPSRPHRPRYDD
                     HVGEEQPNRYTPRSGKYSETDPSTRFSRKPYEDSRPDVHKPFRSSDRYHDDKPKHFEG
                     NRHEFSDGHFSHPKNFRTNSRNFSSDRRERQGSSYNRDFDSNPAQSYRSRTFQGSYGD
                     ERKHIERKNLNTFRERSPQKDNSGEISTNPTGPIRLNRYIAISGLCSRREADVYIQNG
                     QVTVNGKVVTELGTKVNLTDEICFKGKVLKSERMVYILLNKPKDYITTMDDPHAKRTV
                     MELIEGACPQRVYPVGRLDRNSTGVLLLTNDGELTAQLTHPSFKKKKVYQVGLDRKFN
                     PNDFQKLTEGIEIDGEVVKVDAVDYVEGGDGTELGVEIHSGQNRVVRRMFDALGYGVV
                     KLDRVYFAGLTKKNLPRGKWRFLTPKEVNMLKMGRF"
     gene            32526..33407
                     /gene="era"
                     /locus_tag="PK839_01940"
     CDS             32526..33407
                     /gene="era"
                     /locus_tag="PK839_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HOG71526.1"
                     /translation="MTHKSGFVNIIGNPNVGKSTLMNALVGEKLSIITSKAQTTRHRL
                     MGIVNGEDFQIVYSDTPGILKPAYKLQQAMMKFVETALEDADIILYVTDTVETPNKNQ
                     EFLNKLQQLGKPIVLAINKVDLTTQEKVQELFNLWSTMMPNATVIPISALHGFNLNSL
                     VDFILDRLPEGPEFFPKDTLTDKTLRFFASEIIREKILNNYRKEIPYSVEVEIEQYTE
                     SDTIDRIKAIIYVERESQKSILIGHQGNMLKKVGTEARLELEAFLGKKVYLELFVKVN
                     ENWRNDTSKLKRFGYPL"
     gene            33511..34818
                     /gene="der"
                     /locus_tag="PK839_01945"
     CDS             33511..34818
                     /gene="der"
                     /locus_tag="PK839_01945"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008661792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HOG71527.1"
                     /translation="MGNIVAIVGRPNVGKSTFFNRMVGSRHAIVDQVSGVTRDRIYGK
                     SDWNGVEFSIIDTGGYVVNSDDVFEEEIRKQVILAIEEADVILFLVDVRDGITSMDTA
                     VADILRRSSKTVHLVVNKVDNNLLRYDANEFYRLGLGTPMCVSAINGAGTGDLLDEVV
                     KSFSPVHEEVQELEIPRIAVVGRPNVGKSSLINSLVGEERNIVTPVAGTTRDAIDTTY
                     NKFGYNFTIVDTAGLRRRTKVEENLEFYSVVRSIRAIENSDVCLLLVDATQGMENQDL
                     FIFNLIQKNRKGLVIVVNKWDLVEKDTKTTDRYTKLIKERIAPFQDVPIVFASALTKQ
                     RILKVLETASNVYKNRKQRIPTSKLNEFLQATLDIQQPPTYKGKYIKIKYVTQLPTPT
                     PAFVFFCNLPQYIREPYKRFIENRLREQFNFTGVPLQIYFRAK"
     gene            complement(34857..35774)
                     /locus_tag="PK839_01950"
     CDS             complement(34857..35774)
                     /locus_tag="PK839_01950"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOG71528.1"
                     /translation="MISICIPVYNFDVTELVDELLRQANHLNYPVEVLIFDDHSQQYF
                     RRQNSLLASRSKTSYLEFDFNIGRSKIRNRLADFAHGDWLLFLDCDVMVDSPDYLKTY
                     IDNLGRAKVLCGGREYGPKPFRPELLLRWKYGVSRECKSAFVRQTDPYSSFISGNFVI
                     SADIFNAIRFNEALSGYGHEDTLLGIELKRHNIEILHLDNAARHLGLDPCGEFISKTE
                     QGIVNLVRILNISAGNRREIEKSIKLLRIFRFLRHIGMLYPLRWVFKVLTPYIRRQLC
                     SSNPNIQLFDLYKLSLLSKIYRPHWRQVS"
     gene            36146..37192
                     /locus_tag="PK839_01955"
     CDS             36146..37192
                     /locus_tag="PK839_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG71529.1"
                     /translation="MVKFYLSSSLVLIFNMVIPFSGRSILAQDWNKFEGLFIQDTTQK
                     VKGTSNCAFKNISTLSYYPDTLPGWFFSPPVLYQGAAGLGISDPDMPMEKAREQAYLR
                     AKSMALLNYNARVQYYRDIYSSANEVGRYTNLRQRFDTYFKISSLATVSNAMFEVVDT
                     HFTRYNEYVVLLSYNPGNHSDDSLYKLTSVGSAFLVEATFDDVVDEQAEYELLCNATF
                     GEDSAASSHFLFREKGKRFLTSSFMNNAEVGYPMFPYSYVSPQDSARQHPFISYSGLW
                     SSYLRQLVNYLTLNAQQSSIKLKSMDDQYSTKLTNLSREIASFRASLNINGIKFANDT
                     LKLDLQLNEVESNF"
     gene            37260..39239
                     /locus_tag="PK839_01960"
     CDS             37260..39239
                     /locus_tag="PK839_01960"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HOG71530.1"
                     /translation="MKKFNLYMGAIVAGVLMFYSPARSQEFSKIVPKQLGEKITLEYA
                     ISGETPGQLFNITPYYSVDGGKTFKQPKSVKGNVGSNVPGGRNLIVVWDVLKDLEELN
                     SDVIFKLDAKTSTTKPLTDDFNQVHFSLESLNRTKDGNIELLLSITNNGPTRDLKIIN
                     GLVTITDFNKQKYDAQKGNIAEVQGNERYSTPTRTIKQGETVKSRFVFERIPAEMDRI
                     MRLDLGVELITDEKYGIDLKIGKLQFRDLPVSPAPTTGLKVKRDYSFQLTSQPARFDI
                     RKVEIADAIPPEIVVTSPADVPLAGKEATRGRPYAQSSIGMDDRRLRSLASGEEYATT
                     DSSMTVKGKASDESGIYQVLVNGFEAKVDSNGIFEASIPLEIGRNEIAIRAVDLRQNA
                     VEKKFFVLRKAPSGKVAKGETEELDLVFDQPKTAPKFYALIMGANDYQDNSITDLDNP
                     ISDATKLYNVLVNRYTFDPNNVVFLKDPTREQMINALDMLTRRIGKNDNLLIFYAGHG
                     YWDKETDFGYWIPTDSKGNSTANWLANSQIKDYVAAIKSKHTLLIADACFGGSIFHSR
                     KAFQEGSSSTKEAFDAPSRKAMTSGNLTEVPDRSVFLEQLVTRLDGNTKNYVTAEEIF
                     ASIKNVVMSSSPVTPVYGEIKDAGDEGGDFIFVRK"
     gene            39358..39894
                     /locus_tag="PK839_01965"
     CDS             39358..39894
                     /locus_tag="PK839_01965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG71531.1"
                     /translation="MKKILLACLAAAVFLITCIAASAQEDLKLQKLEEKVKIAQEKLD
                     KADAKVNSADSLISAGKSIIQEAEVEVRALEAEKKQLDKAHFAELKPLEKQLRSKDKD
                     EANSAKEEIKASETKYKEDLKGWNASYKEALKKYEAGKKMVQKGEENMKKAKDARKEA
                     EATLKEAEQALSKAEKAK"
     gene            40111..41406
                     /locus_tag="PK839_01970"
     CDS             40111..41406
                     /locus_tag="PK839_01970"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOG71532.1"
                     /translation="MSKSGLRSFPRNFWTVITMEFFERGSYYSVMSILSVYLVLGVNE
                     GGLGFTKESVGVIKSVITPLLYILPIISGAIADRFGYRKILFFSFAVMSLGYLLTSFS
                     NTYATVFLSLILMVTGAGFFKPIISGTIARSTDSRNSSLGFGIYYWAINLGAFLFPLI
                     FVPLLKSIGWSYIFIMAAVGTGWLLFLNLFAYKEPTIPKSNKSLIMVFKEMVLVLKDY
                     KFILMIVVYSGFWVLYFQMFDSVLWFLKEYVDMTPVDTFVNSLLAIFVSNPNWHFDAE
                     HVTVVNAGTIIALQLVISAIVKNTRALPTMIVGIAMGTIGMAVLATSNYAWVFIGGMI
                     VFSLGEMIAQPKFISYIGLIAPDDKKALYMGYSFLYGVIGSGIGGVLGAYSYVKLVNE
                     MGRPSMFWLMFSFIGVATILGLLAYNRFILKKGTANPHS"
     gene            complement(41424..45092)
                     /locus_tag="PK839_01975"
     CDS             complement(41424..45092)
                     /locus_tag="PK839_01975"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG71533.1"
                     /translation="MNKRLVAIVVLVIASLALLLVSQYLSKSALDVRLYRPNVKHLER
                     IVERETTYSGKLAREFLGKVNSNASSRQIFESGVEFKRKLEGKWDGELFLFSHDTLCF
                     WSCKLDVSGVPFDSSQVGLVKIQSEYFFTQWFTAGKGLQLLVLNPIYSEYPYQNKYLK
                     NSFSPLFKEFNNYRISIDLVSNAIPVRPHNVAPFYLVPLDIYQNGHVNRLWPILQWIA
                     FAMAVCAVLLIFGQKFFRYKPLLKIAGATAGIFIIRYLSLYMEIPSRSGMKIFNPEIY
                     AHSFINPSLGDFFINAILVFMLVLFIRQSIRLSLRHIKNMHLYLLAASVLILFLALFP
                     ITDKLLTSLVTHSTLTLEPYRIFSLSVYSIIGYLSVSLWAASAVILTITWVSVVAEQL
                     SLKQRLILLVSGLLVVTLLSLALTSKPSGLGLVWMWLTVAYVLLVYRSSKTHLLLRHI
                     LVIVTIFSAYSVSTVFIKTSEKELNVRKVLAINLSSERDPLAEMLLPNLYQKMLTDSY
                     IRDCIRNIEQKNAELYSYLRKDYFKNYLNRYDLRATVCSPSSIINVDGKSTLLCDRFF
                     GMLIDDYGIMLPSSRFYYLNTQSGFISYVGEINYRIGDNIRNLYIELTSRPGWEMLGY
                     PELLIEGRASQQQLKEYSWAKYHNGNLITQSGEFPFRQKVSGINPTTQVFTCENFLGY
                     NHLTYRPGSSDIVVISRPREKLLNVTASFAYCLFLYFVMFLLCFKIADIPLRLIPTHA
                     SFKNRITRAIILIVTLSLLMVGAATIAYNIKNFEQRNEKNLSEKLISIMFELDRDVDL
                     IQSNDPSAIGFLSDRLVELSNIFYTDINIYDTTGMLLATSRSEIFEKQLVGPRMNPAA
                     WHELATNHSPKFLDNENIGKAKFLSAYVPILSSNREAIAYLNLPYFTRQEELRSELYS
                     IVVAIVNIYALLTLIAISIAIIISSQITRPLQLIRERIGKVSITGNNQTINYSSDDEV
                     GQLIGEYNRMVNELTRSANELARNQRESAWREMAMQVAHEVKNPLTPIKLSLQHLVKA
                     KKDNLPDWDARFERFAQSLLEQIESLAVIASEFSLFAKLPSAKREPVKLLDIINDTAN
                     LYRGYRNVALNVISYMEAEPLVLADKEQLIRVFNNLIKNAIQSIERGKDGKVTITLAP
                     ENDLLIRVDVTDNGVGIPEEVLPKLFTPNFTTKSGGTGLGLAISREIVLGFGGEILVK
                     TQKDLGTTFSVLIPFLKH"
     gene            complement(45156..45629)
                     /gene="rlmH"
                     /locus_tag="PK839_01980"
     CDS             complement(45156..45629)
                     /gene="rlmH"
                     /locus_tag="PK839_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005831223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HOG71534.1"
                     /translation="MKIHLILIGKTSVPYLSEAMNDYLKRIERYCRFEVKVLPELRNT
                     KSMPVEVQLQKEAEVLMPVMGNYQEVILLDEKGRELSSEEFAGFIQKKQNSGLRDIAF
                     VVGGPYGFSDGVKLAAAERLSLSRMTFSHQLVRLVFVEQLYRAFTIINGEPYHHA"
     gene            46087..46512
                     /locus_tag="PK839_01985"
     CDS             46087..46512
                     /locus_tag="PK839_01985"
                     /inference="COORDINATES: protein motif:HMM:NF027349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4783 domain-containing protein"
                     /protein_id="HOG71535.1"
                     /translation="MGKQKIKLLSLFLLIMLGSSASVLQQDSNEIISKSAEALQNGDA
                     VNLAKYFYKTIEIDMLGEENFYSQAQSVQLLKNFFEKNKPVKFAVSHQGAKETSAFAI
                     GQLQTKGNTFRISIFLKSDGSQFFIHQLRIEQDEEGNGI"
     gene            46513..47364
                     /gene="nadC"
                     /locus_tag="PK839_01990"
     CDS             46513..47364
                     /gene="nadC"
                     /locus_tag="PK839_01990"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HOG71536.1"
                     /translation="MATNPELLVEALLNLAIAEDIGDGDHSTLSCIPPSATGKVQLMM
                     KQEGIIAGLSVALKIFKKIDSSVEVEFFRGDGDLLRYGDIAFKAEGKVHSLLQAERLV
                     LNVLQRMSGVATQTREYVNRCEGTKAKILDTRKTTPGMRILDKMAVVIGGGMNHRMGL
                     FDMIMLKDNHIDFAGGIENAVALAHKYLNETGRKLAVEVEARSLKDVQVILRVGGVNR
                     IMLDNFSVEATREAVGMIGGRVETESSGGITLENIRDYALCGVDFISVGALTHQIRSI
                     DMNLKKI"
     gene            47535..48422
                     /locus_tag="PK839_01995"
     CDS             47535..48422
                     /locus_tag="PK839_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG71537.1"
                     /translation="MNTKRLLLISAILFTTILAWILPVEAQNRPPQNVYRVTVTSRYE
                     IKDGKRTTNSRAIRQEIKDSLGRTHTILNRDYESQRVTSHTWHTFSGKIITRTDEFEN
                     ERLIRFHLFTYTPDSLIASEAIYRVQPGDTSLYLNIRYGYTNRRPVQAIATSATGKKA
                     YTLKSEWDSQGTEIKRTVKSKKGFAPLDSIALLTCHPTYDSLGRKVAETISRKFLDGS
                     SKTQQFRYAYDSKGNRIMIEELNPSGSTIARTEMQYNDKGMLKFISRFDAQNNLIDYQ
                     AIRYELYPSRNRLNMIIEY"
     gene            complement(48563..50302)
                     /locus_tag="PK839_02000"
     CDS             complement(48563..50302)
                     /locus_tag="PK839_02000"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG71538.1"
                     /translation="MKAGYRGFLAAAVAVLLVSLVYPSGVFSQDLSQQIANYEQLAAE
                     FEKNGDANQAAYYFDKAANQAWQARDLNRAIANFDRALANIKQVGNVNGAKTIYTNLG
                     LIYCEKGNHNEAYNNFLAALAAARSLGRKDDIANALVNISTEQIEMSDFTGAQKSLDE
                     AQRLAQEVDNARALKNIYFNYNKLYEKLGSKDKADEYFRLYMMLNKKLQDDEFKARQM
                     LAQAKVDSAGRIINQISYEKESTARKLMETSHELQQTTNILKETERISTEQRMQIDLL
                     NTQMKLREAEIRNQHLLQRIYIGLIIILLMFAAILFWANNQKQKANKLLSEKNAEIFA
                     QKEEISRQADELRELNALKDRLFSIIAHDLRSPLFSLITMLNIAKEGHFTEESFKEII
                     SELSSNVSHTTALLENLLTWAKNQMQGTKVKPERFFLNEVVTPRLQQLNEVALQKNIV
                     ITNHVDESTSVYADRDMLDLIIRNLISNAIKFCNAGDKIQIWSSVENSHVKVCVEDTG
                     VGIDAHSLKKLFGSQVSSTPGTRNEKGTGLGLILCREFVEMNNGKIWAESTPGKGSQF
                     FFTLPISGEQASR"
     gene            complement(50710..51390)
                     /locus_tag="PK839_02005"
     CDS             complement(50710..51390)
                     /locus_tag="PK839_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008153932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HOG71539.1"
                     /translation="MKKRLVVLTGAGVSAESGIKTFRDSDGLWEEYDVMEVASIEGWS
                     RNPQLVLNFYNERRRQLQKAMPNPAHLSLAKAESDFLVYIITQNIDDLHERAGSTKVL
                     HLHGELKKARSTVNPDLVYDIGYSDIHWGDMCELGSQLRPHVVWFGEAVPAMEEAVKL
                     TQTAELFAVVGSSLNVYPAAGLLSYVPRQCPIFLVDPKEVATFRDDITVIREPASTGV
                     PKMLRLMA"
     gene            51773..52612
                     /locus_tag="PK839_02010"
     CDS             51773..52612
                     /locus_tag="PK839_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG71540.1"
                     /translation="MRKLALLILFCILSELGLYLQAQPLVEIYLNSRGSESRGPAFKS
                     VEELHEVLVLDQAKTPSFRVVKQFNAQGKVVSEIKYNSAGGVQNEVRWEYNINGYPIR
                     KTTHQFINYKGWVNEEITLKYNDTTGYLNEINKSVESKIINTALVSCDSTGRIVEVRV
                     FNEKGELSGIDRLIYVPANNLIRVLSYKANDQFVMATAYPLDPDKPEPKSTLKREFNS
                     HGDVILELLPGSKIGQGYYYEYSYDTFGNWTEKLTYQCTVTPNGNVKDKKLEHRITRK
                     IVY"
     gene            52706..53125
                     /locus_tag="PK839_02015"
     CDS             52706..53125
                     /locus_tag="PK839_02015"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002561924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HOG71541.1"
                     /translation="MKILILCTGNSCRSQMAEGFLKLFGGNFEVHSAGTQPAPAVNSY
                     AIKVMGEVGIDISHNRTHHVDEFLNEEWDYVITVCGGARESCPAFIGKVKNRLHIGFE
                     DPAEAQGSDEEVLTVFRRVRDQIGEEFRKFHDNLLKP"
     gene            53130..54194
                     /gene="arsB"
                     /locus_tag="PK839_02020"
     CDS             53130..54194
                     /gene="arsB"
                     /locus_tag="PK839_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003002381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HOG71542.1"
                     /translation="MPSKSRLKFFDRYLTLWIFLAMLVGVLSGYLFPSIAVFWDSLKA
                     SPESTTNIPIAIGLILMMYPPLAKVKYEELGDVFRNTRVLSLSLIQNWVIGPILMFLL
                     AIVFLRFNIDYMYGLILIGLARCIAMVIVWNELAGGDTEYAAGLVAFNSVFQVLFFSV
                     YAYLFIAILPGWFGLPVNETISQITIGQIATSVFIYLGIPFIAGFLTRLVLTRIKSKA
                     WYNTHFIPKISPLTPIALLFTILVMFSLKGEYIVKIPLDVVRIAIPLMIYFVVMFLIT
                     FFMSRRIGASYRQTATLSFTAASNNFELAIAVAIAVFGINSGEAFAAVIGPLVEVPVL
                     ISLVNVSLKLKKKYFSQQAV"
     gene            54454..58269
                     /locus_tag="PK839_02025"
     CDS             54454..58269
                     /locus_tag="PK839_02025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOG71543.1"
                     /translation="MASRIVLLVTNKVLPSFQFEPRLDGMGYSLITLDRIGSDVELPA
                     FDLAIVGVADRASYNAILKVFRKYPEKPLLFYSGKNTADAQKSIPELDSTNHVNPDFT
                     NHELSLQVELAITRKWFDHSSIFSDGNAGSNHVRLKKILSEEQAWRVLFEQSPNGVLL
                     SDSAGSIIFTNNATSEILGYSSSQLLKMRFHDLVPPETLPQVDQNIKKILAGEQLITE
                     VYNLRKDGSKRFVQLHESRVLFPSGEFGIMVVSIDMTRAKKAEDALGESLEKYRILVE
                     QSNDGIIYSKDGIITYGNPRIAELLEVQHCELVGKPFYTYLPKSEWEKLQNRYKNRLE
                     GIKEPSIYETLLVSAHGSNIPVELNVNLATIGNERVTMVVVRDISLRKEAEKAIRESE
                     ESYRGLFNNSGEAIYIIDASGILLDVNPSGLKLFGKEKEETLGLTLMELADQEKSSTE
                     EVKRKIRIAHQGQTQYLEFWGKKSNGDRVPMDIVLSKGTYFGLQVVLAIGHDITERKN
                     AEAILRESEDKFRTLTEQIPVGIYRLTPEGKILYGNPAFAKTLGFNDTSEVIGHSMDE
                     FIATNYDDWVEESVKLSPGAPAEQSFRRQDGINLWANNQLRRVKNASGEVDYLDGVLV
                     DITEHKFTLDALKESEERFRAMVTAIPDRLFRINADGDLIDFAPSEQLLDFELNAGMI
                     GHSLSEFLPQDVFQKFTDAISLAKQSGKLQTFEYKLSKNNQDYFNEVRLLQSGRSLFL
                     VLQRDITQKKQFEAQANMLAQTIMTANDSISITNTDGHFIFVNPAFTKTYGYTQSEII
                     GKHTSILKTPDRDLTLDKTIHEETLKGGWQGELINVRKDGAVFPIFLSTSAVYDENGI
                     AIAHVGIANDITERKKIEQELIRAKEKAEESDRLKTAFLSNMSHEIRSPMNAVLGFTQ
                     LIKSDEQLSETGMQYIELIQNSGKQLLSLLEDIIDISKIQSNQLRIDKNAFDLNELMD
                     ELYLIFSNQLKLKDECKTLLFKPEKTCVSPFVIYSDPVRIKQILSNLLSNAIKFTPSG
                     SINFGYSIFSDDDASKIQFYVRDTGIGISIENQLLIFERFRQADNSYTRLYGGSGLGL
                     AISRGLVELLGGDIWVESKPGEGSEFFFTIPLEFSEKDTPAPVQPSSEVKQPVSQLKG
                     KTVLVVEDVNEIRLYFERVLERTGAKILFAKNAKEARKLFKSNNRIRLVLLDIGLSDA
                     DGFELAVEFKRMRPNTPIIAQTAYALQSELNRSIESGCDDYITKPLDSAVLIGKMLRL
                     IGSKSQQSD"
     gene            complement(58573..59733)
                     /locus_tag="PK839_02030"
     CDS             complement(58573..59733)
                     /locus_tag="PK839_02030"
                     /inference="COORDINATES: protein motif:HMM:NF040309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6051 family protein"
                     /protein_id="HOG71544.1"
                     /translation="MHDELEIVEYNFESSSHKILPGVNKYHCTLHNLDIECNYTYSTE
                     VGNPNSNVDTKDICIEENRFYKYQIIKPKGEATTQRVVFLFHGFNEKDWGKYVPWAKA
                     ISESTGFSVILFPIAFHMQRAPKGWSNKREMFKLSEMRKRSYPNVVHSTLSNVAISIR
                     LHSMPQRFIWSGLQTYYDVIQLIEECKEGKHPYISKNFEFDFFAYSIGGFLAQILKLS
                     NYNNYFANSKVCLFCSGATFNRLSPVSKFILDSEANIALYSFLIEHFDSVLQKDNLLH
                     HYITEHLEGQVFRSMLNYQRMRDLREGLLKKYEDQIYAITLKKDLVIPSFEVINTLKG
                     AYRTINIKVEELDFNREYIHENPFNADSGTSQLVDEDFKLVFSKVSDFFKKG"
     gene            complement(60223..61011)
                     /gene="pdxH"
                     /locus_tag="PK839_02035"
     CDS             complement(60223..61011)
                     /gene="pdxH"
                     /locus_tag="PK839_02035"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HOG71545.1"
                     /translation="MLQYKKQGVEAEIITPVRVEQLPATFVSGLMLLCYFRATESKDV
                     IIFSGTMGDYIQLRREYMLKSLRKTDVLPNPFEQFDIWFKEAVNAKLPDPNAITLATS
                     TFEGRPSARVVLMKHYDSDGIAFFTNYESRKARNLLQNPYAAMVFFWPELERQVRIEG
                     KVSRVSEQQSDRYFQTRPEGSKIGAWASPQSSVIPNRRYLEQLHADFMEEFQGTTIKR
                     PPNWGGYILQPALFEFWQGRENRLHDRIQYSLVNEKWLIERLAP"
     gene            61202..61774
                     /locus_tag="PK839_02040"
     CDS             61202..61774
                     /locus_tag="PK839_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG71546.1"
                     /translation="MDVETRTTAGQVMGVIGIVLGVVSLVLAFIPCIGVMAFIPGVLA
                     LTFSIISIVQASNGYGSKGLGIGALIVSLVSIVLAAAWLIVFSGTFAFADRAIRNSDK
                     IEIFGKEFGKAFEEEFGKGDVNLKIVSDSLENSLRQLENELDSLDGYIPEEKARKAGQ
                     AAARALKKSTRVVIETDSGKIQFETSNKSH"
     gene            61780..62211
                     /locus_tag="PK839_02045"
     CDS             61780..62211
                     /locus_tag="PK839_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG71547.1"
                     /translation="MKPPSCKKGINVYASVNALFIVVILAVFMYSFAFKTEKYPIPAL
                     LTQSTGLIPPSRGLSKSFSEIVRGNISAAKLINPHSLQIFAFFLIQLVMRVAGFAAAK
                     IIGSPFPVKGIAVDVCISLALFVFCFAPLISYTFRLFVQLF"
     gene            complement(62191..63045)
                     /locus_tag="PK839_02050"
     CDS             complement(62191..63045)
                     /locus_tag="PK839_02050"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOG71548.1"
                     /translation="MKISQFNISNFKVDGGAMFGVVPKTMWGKVYPSDENNLIPLSLK
                     SLVVEANGRVVLIDNGIGSKQDAKFMSHVYAFGGEGLLPGLAARGYRPEDVTDVILTH
                     LHFDHCGGGIFRDSHGKFQLTFPNALYHVSAAQWEWATHPNSREDDSFLPENILPMKE
                     LGALHLIESEGELIPGLSLRIFNGHTRGQLIPLISYRGKTLVFCADLFPFTAQIPLPW
                     IMSYDVEPLKTLQEKEQFLNEALRGNYILLYEHDYFADCSRLQLTAKGIRATEGFSFQ
                     QLLKELNE"
     gene            63456..64994
                     /locus_tag="PK839_02055"
     CDS             63456..64994
                     /locus_tag="PK839_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007482875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HOG71549.1"
                     /translation="MLVKTFGSAVHGIKATTITIEVSVSQGINFFLVGLPDNAVKESQ
                     QRITSALETNGYRFPKKRVVINMAPADIRKEGSAYDLPLALGILAASEQVNPQMLPDY
                     VVMGELSLDGSVQPVRGVLPIAIQAREEGFKGFIVPIQNAREAAVVNDLDVYGVSSIR
                     EVIDFFNGNESLKPTIINTREEFFANANSYDVDFADVKGQENVKRALEVAAAGGHNII
                     MIGPPGAGKTMLAKRIPTIIPPLTLREALETTKIHSVAGKIDRDTSLMTRRPFRSPHH
                     TISDVALVGGGQYPQPGEISLAHNGVLFLDELPEFKRTVLEVMRQPLEDRVITISRAK
                     FSVEYPASFMLVASMNPCPCGNYNNPFKECVCSPGMVQKYLNRISGPLLDRIDIHIEV
                     IPVPFEKLATTINSETSETIRKRVIKARQVQEERFVEWKEVYCNAQMTSKLIRKFCEL
                     DSAGSSLLKTAMERLNLSARAYDRILKVSRTIADLDDSPSIKPEHLAEAIQYRSLDRE
                     NWAG"
     gene            65219..>67395
                     /locus_tag="PK839_02060"
     CDS             65219..>67395
                     /locus_tag="PK839_02060"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014776353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HOG71550.1"
                     /translation="MIISNVFWVVPIASVLALLFAYYFFRQMMKEDEGTDIMKKIALH
                     VRKGAMSYLKQQYKVVGLVFVVLVLLFALLAYGLHVQNGWTPFAFLTGGFFSGLAGFL
                     GMKTATYASARTANAAQKSLNRGLKIAFRSGAVMGLVVVGLAVLDISLWYLILNYFID
                     EPDPSQKLIVITTTMLTFGMGASTQALFARVGGGIYTKAADVGADLVGKVEAGIPEDD
                     PRNPATIADNVGDNVGDVAGMGADLYESYAGSILATAALGASAFALFEGNMQYKAILA
                     PMLIASVGIVLSLLGIFIVRTKENATMRDLLRSLGRGVNVSSLLIALSTFGVLYGLGL
                     HHWLGISFSVVSGIAAGVIIGQSTEYFTSHSYKPTQRVSESSKTGPATVVISGIGLGM
                     VSTAIPVITITLAILMAFLCAINFDVKHMLEAQNIQLGLYGIGIAAVGMLSTLGITLA
                     TDAYGPIADNAGGNAEMSHLGPEVRKRTDALDALGNTTAATGKGFAIGSAALTALALL
                     ASYIEEIKIGLVRIGENVLTFANGETVETAKATIVDFMSYYQINLMNPIVLAGIFIGA
                     MMAFLFSGLTMNAVGRAAQKMVEEVRRQFREIKGIMTGETEPDYERCVAISTRGAQIE
                     MILPSSLAIIVPILTGLLLGVAGVMGLLTGGLGAGFVLAIFMANSGGSWDNAKKYIEE
                     GNFGGKGSDCHKAAVVGDTVGDPFKDTSGPSLNILIKLMSMVS"
BASE COUNT        18907 a        15727 c        14870 g        17791 t
ORIGIN      
        1 ggttcttaat gcttaattac atcaaacccc ctgccatata ctcccatcac ggtagaaacc
       61 gaaaggaatg cttcggggtc gatttccttg attatcttgt aaacatcatt tgattcatat
      121 tttcgcacca gggtaataag caccttttgg ggctccttgc taaaccagcc taccccgtca
      181 accaccgtta ctcccctgtg caagtccccg gtaatcctat cggcaatctc ctgatatttt
      241 ttggaaaaga caaaaatttg cacggactgc ttgctgcccg aaagagttgc atcaatagca
      301 taagcggtaa ttgccatggc tacatatcca tacacgatag tttcaattct tccggccgga
      361 tccaggtcaa gcagcgcaaa gtatgaggat ccaataatga taacatcgca gtaaagaata
      421 accctgccag ggctaatgtt gcggtactta ttgattatca tggcgataat gtcggtaccg
      481 cctgtgctgc ctccctgggt aaatacaata cccaacccca caccggcaat ggcaccacca
      541 ataataaccg acatgaactt gtcgtcaact acgggttgct tgataacaac ttgcagaacg
      601 gaaagaagca ccgcccccac agtaacacca aatatagttt taacaccaaa ccccctaccc
      661 agaattttaa tggaaagtaa gattaaccca atgttgatga cgaagtaggt gtaccccatt
      721 ggaattcccg tggcatagaa caccagcgct ccaattccgg aaacgccgcc tcccgtaagc
      781 ttgtgtggta gcaagaacgc tgtccacgaa agggcatata acagcagccc caaaacaatt
      841 atgctgtagg agcgaatctc cttaaaaagc cttttccccg tcatgtaccc tgaattgcag
      901 gttaaatttc aaccctttca tcgtcctctt tcctacgttg aattgactta acgtaggagt
      961 tcaggaggaa gctggcctcg ccaattgcgt tttcgagatc ctctgcatta aaggtatcca
     1021 gcaggctcaa atccgtagct agtataatgt agtagcgaca ctcttcaagg gcatcctggg
     1081 cagctgaaag gtataggagt ttttcatccc tgtccttctt tttatagcca cctacaatat
     1141 tagtagcaat agccatggcg ctttcgcaaa ggtaatcgcc taccatttgc tgctgatcct
     1201 caggaaaagt tttaacctgc tgaaaaatat tcaacaccag agcgtgggcc ttttgccata
     1261 caattagatc acggaacgat ttggttttgt tttcagattt ttccatcttg ttaatcttta
     1321 gaaaattaga atttgaaagg ttgctatatc gctatagcta aacgggtgaa tacgaagata
     1381 cttaaaaaat aacatacaac aacttctaca caacaatatt aacaatttta tggggcacca
     1441 ccaccacttt tttaggggtt ttgccatcaa gctgtcgctg ggtgttgctg tcagccaata
     1501 ccagcttttc aacttcggcc accggcaaat tcagagggat gtcaagagta aaacgcattt
     1561 ttccgttgaa tgaaatgggg caggtaaagg tgctttccct cacaagctcc tcgttaaaca
     1621 atggccagct tgcatcgcaa atggaaccgg tataacccat ctggctccac agttcctcgg
     1681 caatgtgggg ggtatagggt gaaattagaa tgagtaaagg ctccagcacc tgccgtttat
     1741 tacacttaag gtcggtgagt tcattcacac aaatcatgaa ggccgaaaca cccgtattga
     1801 agctgaagcg ctcaatgtcg tcggaaacct ttttgatggt ttgatgcagc accttcaact
     1861 cctgcggtgt aggattgtca tcgctaatct caagctggtt ggctgggtta tggaataacc
     1921 gccagaactt tttaaggaat ttatgcaccc cgtctatacc attggtatcc catggtttgc
     1981 tttgttccag ggggcccaaa aacatttcgt acatgcgtag ggtatctgca ccgtactttt
     2041 caacaattgt atcgggattt accacgttcc acatgctttt gctcatcttc tcaaccgccc
     2101 agccgcaaat gtatttccca tcctcgagga taaactcggc gttgctaaat tcaggcctcc
     2161 attttttaaa tgcctcaaca tcaagaatat cgttgttcac aatattcaca tcaacatgga
     2221 tgggggtaac ctcgtagctt cccttcaggt taagggaaac aaaggtgtta gtacccttaa
     2281 tccggtaaac aaagctggag cgcccctgta tcatcccctg gttgattagt tttttaaagg
     2341 gttcgctttt gcaaacgtag ccaaggtcat agaggaactt attccaaaag cgggagtata
     2401 taaggtggcc aacagcatgc tcggtgcccc caatgtaaag gtcaacattt tcccagtagg
     2461 tgtttgcctc tttcgacacc agctggctat cgttgtgcgg gtccataaag cgaaggtagt
     2521 aggccgacga accggcaaat ccaggcatgg tgctcagctc gtaggggtac ccctcggggg
     2581 tatgccaatt ctttgctctt cctaaaggtg gttcgccttt ttcggttggc aaaaaagcat
     2641 caacatccgg aagttcaagg ggtaacttct cttcgggcaa ggggtaaggt aatccatcct
     2701 tatagtaaat tgggaaaggc tcgccccagt aacgttgacg gctaaaaatg gcatcgcgca
     2761 agcggtagtt caccttgctt ttacccaacc cccgctgctc aatgatttcg catattcgtg
     2821 taatggcttg tttgacttca agcccatcaa tcagggggct gttcaccacg atgccatcct
     2881 tcgcgtcgta tgattcctcc catgtttccg gattggtagg ttgttcgccc ttcctaacaa
     2941 ctgtttgaat aatagggagg ttgaaagttc gggcaaaggc gaagtcgcgg ctgtcatggg
     3001 caggaacagc catgatggcg cctgttccat atcccgccag cacgtagtcg ctaacccaaa
     3061 tgggaatggg ctggttggta aagggattca ccgcatagct tccggtgaac tggccggtaa
     3121 tttttttggc ctcggccacc cgctcacgtt cagttttgcg tttcacctcg ttgcagtatg
     3181 cctcaacatt atcggcatac tcgggtttgg taagaccatc cagcagctcc gactctggag
     3241 caagcaccat gaaggttgcc ccatagatgg tgtcgggacg ggtggtaaaa attttaattt
     3301 gctgccggct atcggccacg ctgaaaagca cttctgcccc ttccgagcgg ccaatccagt
     3361 ttcgctgtat ttccttaagc gagtctgtcc agtccagctg ctctaaatcg gaaagcagcc
     3421 gttcggcgta agccgaaatg cgaaggcacc attgcctcat cttacgctgc tcaaccgggt
     3481 gaccacctcg gatagaaacg ccctccttca cctcatcgtt ggccagaacg gttcccagtg
     3541 ctgggcacca gtttaccata acatcggcaa ggtatgccag tcggtaagac agaagaagtt
     3601 cctgctgctg cctgccatcc atattcctcc aatctgaagc agtaaaaatt ttggtttcgc
     3661 tacaagcagc atcaacatgc tgattgccgt ttcgctcaaa ctcggctacc aactcatcaa
     3721 ttggttcggc cttctgtgtt ttgttgttgt accagtgctt aaacatttga ataaatgccc
     3781 actgagtcca tttgtagtat gatggctcgc aggtgcgaaa ctcccttgac cagtcgtagc
     3841 tgaacccaat tttatcgagc tgggagcggt agcgctggat gttgttctcg gtagtaatgg
     3901 cgggatgctg tcctgtttga atggcatatt gctcagcagg aagtccaaat gcatcgtacc
     3961 ccatgggatg gagcacgttg aaaccctgta ggcgtttata gcgtgagtaa atgtcggaag
     4021 caatgtagcc aaggggatga cccacatgca aaccagctcc tgaagggtag ggaaacatgt
     4081 cgagcacata aaattttggc ctgttggggt taacatctac cctataggta ttcctctgct
     4141 gccaaagcgc ctgccacttc tcctcaattg ccttaaagtt ataatccata gaaaaccaga
     4201 ttggttgggc taaatttagc tagcaaagat agctaaaatg agcatttcgc ttaacattca
     4261 atgccggttt tattatgatg tacaggagaa tgccgacatg tcgccttcgc accttcgcat
     4321 ctccccggat gaaaaatacc caaccgcaaa ggtgctgaag tgcttttcca aacaagggaa
     4381 attttatcct ggcgtcttcc ttggggaaaa aatattttac ggggctgcta aggacaaggc
     4441 tcaacaggac tactccccca cctccttcaa aattttctca cccagtccaa tgctataccc
     4501 ctcggagaaa aacgttatct cccccttgcc attcaccacc gctaccacag ggtaatcggt
     4561 tgaaaggggt ttcccacaag caatagcaag cgcattagcc aaactttgct gggaatcata
     4621 tccaatagta acgccaatgg gcaagggcac cccctctaaa agcttttgag cgttgaagcc
     4681 agatggtagc ttatccttag caatagctag tacaacagca ccgccccatt tgtcgagctg
     4741 cgttttgatg gccgcaaggt catttaccaa atgtctggta ggttccttca ttggatctat
     4801 aatggcaact accgttgacc ctcctcctcc aatctcgccc agattcttga tatagcttcc
     4861 ttcaatatcg ggaataagcg ggtcgcctaa atttccaagg acaaccgttt taccttttga
     4921 cgatgggaat tccagcgtta ccaaggtttt ttcatctgga taaatgctga aatactttac
     4981 cctacagctc accgctccgt tttgatctct agtgccgcaa accaacctgt aaagtccttc
     5041 ccttagtgat aggctaaaag gcagggtact aagctgggca tcgttctcaa aatcaagggt
     5101 aacaaacctg ttgttttcaa atcgggctat ggtgtaatgc ttatagtacg acggtgaagc
     5161 aatctccatg ccggaggtag cagaaagtgt aagtgttccc cagccggaag cttcaacttc
     5221 cctttcacca aaatcaacat catgccagct gccgcgcatg aggtactgcg gacgtttggt
     5281 agaaggttca agccgcgcag gaatgccaaa actgcggcaa gccgctacaa agaggatatc
     5341 gcgggagtag gcatcggcta tgcctaacct aactacaccc tcggggctaa ttggaacctt
     5401 atagtagttt tcaaccgaat caattttgat gttctctgaa atccacttga ccaggttggt
     5461 tggatcgttc cggaaaaaac ctatctggtc gaaggtgaaa tagccctgca aaaactcccg
     5521 ccagggtgtg atgaactccc ttccaaccct gggggaaaga atgtaccggt caaataaatt
     5581 gaagctacgg ctatcgagca atgaggggaa ggagtcaaca gttttaagat ggcttcttaa
     5641 agtagcagcg gtaatgtcgt gcaagtcctt ctccgccaga attccaagta gagccatgcc
     5701 cacaggtgtt tcacccggct ccatgttggt aataaaattt tgaatttccg gccagttccc
     5761 ccgactttta gagaggaaat gccatgcctt ttcggcgtta atacccatac ttgctgcaaa
     5821 cttaacagct gacagggaat caataaaagt agcaatgtag catcctcgaa ggctatcctc
     5881 aacagctagc cggcggctgt ttgcagcaac cttatcatcg gcagcagacg taaccgaaat
     5941 ttcgtcgggt ggaaccagca acaggtctgt agcttctgcg gaagtcgttg aggagagctt
     6001 caactccatt tcgttcagac ttctatcaac cttgacaaat gcggttttcc catccctatg
     6061 cgcataaacc atcatatcgc ccaatcccgt agtaatgcta cacaggccat ttttatcggt
     6121 aaccttagag gcaatagggt aaaactcggc atagttgtat atttgaaact caaccctagc
     6181 gccctcaacc ggttgattag cttcgtcaac cacagtaacg gaaaggtttt tggatggggc
     6241 atatcgtggc aacaggttta tctcggcgaa gcaatccgat tcggtaagtt tttcctcttg
     6301 gccattatac ttaccaaaaa caaaggttcg gatcatcatt gcacgtttgg ctggtgctgc
     6361 aaaccacccc atatcgagtt cgggttcggg ttcgcaggct cccaggaaat gccagctgcc
     6421 atcaacccat acctctaccc acgcatgatt atcgtcggta tgtgcccatc gtggagtgta
     6481 aacctgacgg gcgggaatac ccaccgaacg gtaagctgca acagtaaagg ttgattcctc
     6541 gccacacctg ccaaaggcag ttctgacggt tgttagcggg ccgctggtgc gctcatctgt
     6601 tggctggtag gtaacctttt catggcacca gtggtttacc tccagggcag cttcgtatgc
     6661 ggataacccg gataaccttg gttttagctc ggcaaaaaac accttgcggg cagaatcggt
     6721 gtactcgtta ttcacccggt agggcagcac aaaatgcaga aaaatgtcgg aaggtacttt
     6781 ccttccccat ttaaagtggt ctttagcctc cagtgccagc tgtacctgct ccagcacata
     6841 gctgctgctg tgcatggcta aatcgctgag cggcatgtag gcatacaaaa attcaaaccc
     6901 ctcccttgtt ttcaggggta agtcgtagga aacaattctt tgaatggatg tatcgtatgc
     6961 ctcaaccaac ttgagccgtt cgctgaagcg ctgatgaact tcttcccttt gagttttgcc
     7021 cggaattaag tggcggttga accagttctg gcatgaagtc agcatcaaaa atggcacaac
     7081 actaacgagt agaaacttat taatccgcat aatttaccaa tattaagatt atcagcatgc
     7141 tattgaaaca tattcactct cggggataat atataaataa aaatgaagca cctctccatt
     7201 caaatctggg acaatcccta gcaaacacta ttatcctgta aattaaaggg aaaaactata
     7261 atggctaagc aaatataatc tatttgcagc tggaaaaaca ataaggtggt taaaaagacc
     7321 ttgatctagc catatttaat ataacctggc tggcatacgg aaaattatgc agggcttttt
     7381 acggaaagca tgatgtgcgt ttttttaggt tgactatgaa caccccacgt ttgcaaaggg
     7441 taaaccaaaa cccttatcag gttctttagg catctcatca ccaaagcagg tcatgcctct
     7501 ggtttagtgt tgtattgccc accaggtaaa atcaattcta aacttttttt gtctgcgtgt
     7561 actcaatcca gaactacaac ccggggatgt caatgttggt agtaaaatcg ccccaatcaa
     7621 ccttaactag gggtttatag gaagtaccgg aaattttaag ggtcaactcc aagtttgaca
     7681 ccctaacatt tgttttggta gaaaacttgc tcagcgcatc ggcagcagtg tcgaacaact
     7741 cggtaggcac tgccagcttg gctttaaagt taagagactg gtcgaaaccc atgtcgccgc
     7801 ctacactcat tttagcacca cccacgctgg tgctaaaggg ttcaatggct attctgcttt
     7861 cgttaacctt aaagttaaca tttacattgc tcatgtaagg gctacggaat tcttcctttt
     7921 taaaaacatc tgccaacctc ccgaaaactg cagactcctt aaagcctatg ctcttggact
     7981 ggagtttacc gctggctaca atggagctgg gcacgggcat catggatgag tcgagcatgg
     8041 aggcaatgct gatacttccc gatagcttac ccgaaaggtt cttgaaagtt ggaacaatac
     8101 gctgtagtga ggttaatgat ttaaatgcca acgggatatc aacgtcctta atggacaaat
     8161 cgagattaac tctggggaga ctaacatcaa tgggattata gataccatcc agagccacat
     8221 cacctccaag catgttcatg gttaggttgt acaggttgac tatgccgctg tcagcctcta
     8281 catttccctt caggttcgaa atgtcgagtc tgccgtagag taggtgctga acgcttgcgc
     8341 tcaaagtaaa gttaaagttg cgaggaattt taattacacc atgggtaacg accgaatcaa
     8401 ccggctgttt agcctgaact ctaaatcccg atagcagctc gttagcatcc aacagggttg
     8461 atgtgagatt caattgacct tgcagggctt cattcctaaa aacgaacggt ataaaatttt
     8521 ccagcctacc gcttaactgc atatcgctgc ttcccagact tgatttaagg ttttgcagct
     8581 caacgaattt tggtgaaaag ttgacgatta gctcacctac cttaacctgc cgggtagaat
     8641 cacatccggt aagagtgaag ttggaaatgt taagggaacc ttcagcgttg aacttgtcgt
     8701 actccttttc cttcaagtgt gaaatacgtc ctccaaactt caggttggtc tccagcaggc
     8761 cggtaacgga aatgctgtcg aggggaataa catcggccaa ggtagcaaag ttaaccttcc
     8821 caacaacctt gccatcaacc tgcatgtcgc ttacaggcgt ggccacatga aggtaggcgt
     8881 cgaaggggtt gccgcccagg ctaaactgaa gcctgtttac atcaactgtt gatttatcgg
     8941 ggtttgaacc atcgaaccaa accttagcat caaagttgat cccgttggct gattttggta
     9001 gcttggggta tttgagcatg gcattggcca ctgcaatttc gattgatgcg ctgggcaaaa
     9061 tggagtcgtt gtaaaccccg ttaacgcttc cataaagttt cagctggccg gaggtttgaa
     9121 tatcctcgta gcgctttagg tagaatgccg gtatcaggga aagcaggtct ttgaaatcgg
     9181 ttgaaggagc gctaaagcta acatccatac caatgctgcc tttgtgcatt cgcatcatgc
     9241 cctgaaaaag taaggttaac tgatttatac gcagctcatt ctccctgaac gatagggtgc
     9301 cgttatcgag gttgaccccc atgttagcgt taaacctaac ttcagcatca cgaatgtagc
     9361 gtatgccttt tacccttaaa tctaactcat cggccgagga tttaagcctg atttcggttt
     9421 gccttttaac catgttgccc gaaatcaaga ggttcacgcc ccgaagtgtc gtctccatgc
     9481 caagtgaatc atcagcatag aaaaaagtgg cgttgttaat tttaaaccgg gttagtgcaa
     9541 tcctgatgtt ggttggctta gcggcagctg tatcctcctc ctcggtttga ggcaagcctg
     9601 aagttttgac aatgctccaa tttttgctgc catcggcagt aaccctggcg aataccttgg
     9661 catcacttag gtaaaccgac ctcacctcaa tttcctccat atcgattatg ctccataggt
     9721 tcacccgtac gccaagctct ccgaacgata caagggtatc gccagcaaag cgttcaacac
     9781 caacaatgct taccccttta agccccacaa ataaattcgg gaaacctctt aaaagcgaaa
     9841 cctgaaaatc gtcaaattca actgtagcgt taatattttt attaatttcg gttttggcca
     9901 gctgcttaag ctgaggttta aaaaccagtg gtaacaccac agccgccgca attaaaagaa
     9961 aaacagctac ggctaaaatg cggaacagcc attttgcaac ttggctcatc tttttgggta
    10021 aattttaggt taatgccatg gagatgatta gtgatgatga agattgaaac aaatttagac
    10081 aaacttttaa ataagcccga atatggttac acaacacgac aaataattat agaaaagttc
    10141 ttcctatcaa tgtgttaacg taatcaaaag tttaagtttg ctacttgtag ctgcgcttcg
    10201 catgtagaac cagagtcaac aggctatcat tcttgacgag aacaaagggt aaaaggaaat
    10261 tttagatttt tttcagggtc aaaataatag atgcattcgc aattttcttt aagtttaggg
    10321 caaccattaa actcaggcat tgcctaaaaa tcaaataaaa gttaaccatt acgatgatga
    10381 aaaaaatata cctgatttct tttgctgccg ttgcctggtt atcggcctgt acaacaggcg
    10441 agcacaagtt ggcaaccggc ccctggctgg gggttatcca gctcgattca acaggcggca
    10501 agctggatgt tcccttcaac ttcgagctta aaaatgattc cggaacctac tccatgacca
    10561 ttcggaatgc cgacgagctg atcaacgttg atgaagtgga gctggttgga gacactctta
    10621 gggccaggtt gcccgttttc accagcgagc tagttgtgta ctgcggtgac tcactaaagg
    10681 gattctacta tcctaaaggt gttcaagccg ggacgcgata caaattctat gctcttgccg
    10741 gtcaaataga ccgattccca tggtattccg aacctccagc cttcaacatc actggaaggt
    10801 ggtggtttat tgaaaatccg ggctctcccg attccaccgt catggttgcc gagttaaagc
    10861 aggatggaag ccgcgtgact ggtacaattc ttagcactac cggcgattac cggtattttg
    10921 aagggaaggt atccggtaac cgattctact tctcaaaaaa tgatggcgcc caaaccatta
    10981 ttgtaaaggg tgaaatatcc gatgaaaaca ccatggttaa cggggtaatt tcgggtagtc
    11041 caaaatgggt tagcccgtgg agagcagcaa aagatccgca ggctaagctg cccgaagccg
    11101 ataagctggt tcgggtaaaa aagggctttg acacctttga gtttgcaggc aaggatatta
    11161 gcggcaacat ggtgaaatcg tccgacgaaa agtttagagg taaggtgctg gcggtgctgg
    11221 ccggaggcag ctggtgtcca aactgccttg acgagggacg ttactacaag caactttacg
    11281 agaaataccg tgatcaaggt tttgaggtag tttcgctatg ctttgaggat aaaactttta
    11341 aggaatcaca accaaaaatt gagcgttttg caaaaagcat tggcgcagcc tacacctttc
    11401 tgtacgttgc ccctcgtggg cgcgagcagc gtgactcggt gctgtacgcc attgaagggc
    11461 aaatggctta cccaaccagc atgattctgg accgaaaagg gaaaattcgc agggtcgaaa
    11521 caggcttttc aggcccggga accggcgagc actttgttac gtttgcaaag gaaactgaag
    11581 agctggtaaa attgctgctg gaagagcggt agaccatcag ctcctgccta ccggttcgct
    11641 caagaatcca gctaaaatcg acgatagcaa tgcagcctca ccaggtcgaa tccagcgggt
    11701 tgccattcct acttccgaag ccatcatgat attttcctca gtatcatcaa caaaaagggt
    11761 ttcggcagga atcaacccgc tactctcaac caccaggttg aatatctccc gctggggttt
    11821 gcgcaatccc acttcaaagg aatagtaagc cttgtcaaag tagctgctta gcgagcgacc
    11881 tccagcaatg gcttcgagtt tttgattgta actgctgtag tgaatgctgt tggtattgct
    11941 aagcaggtaa accctgtagc gctgtctaag cgcctcaacc agctccagcc tggctacgtc
    12001 ccagtcaagc agcatggcgt tccacgcacc tacaacctgc tcgtaggaaa gaggcattcc
    12061 aaccagggca cacatctccg catgaaaagc tgaaggagaa atctgaccac agtcaaaccg
    12121 gttaaaaata cccatcgagc cggcagataa caggtggtca tcatccgcaa caccaagttt
    12181 cttaaaagcc ataactgctt tgctgtagtc tatatcgaga agaacaccgc ctaaatcgaa
    12241 tacaatattc ctgatattac ggatggaaaa acttgttgag ctactttcag ccataatttt
    12301 ttaatccctt ttgatgaaat atcagcgcaa atatgtaata ttgcactcca aataaaaact
    12361 gtagaagata tattttttgg ggcccatagc tcagcaggtt agagcatctg actcataatc
    12421 agagggtcgc tggttcgagc ccagctgggc ccacccaggc caccgcaagg tggccttctc
    12481 aatttttaaa ggactgtgct gtaagggtgt tgaacaacct ataattaagg tggggtttaa
    12541 gctgataaaa caccctgcat ccgaatgggc ttagcctcag acaagtagca gccagctact
    12601 tcttccttct tttacttgta gaacgggaca tggaatgcgt tttactaccc gaaccctttt
    12661 tagccctttg tggcctgtta acttgtagct gggagttgcc aggatgtacc tccccatcaa
    12721 ccagcctgaa atcgagctgc tttttggcca ggttagttcg ccaaacctca acttttaccg
    12781 ggtcgcccag ctggaaacga cgtccggttc tttttccaac caaggcataa gtttcctcat
    12841 caaactggta gtagtcgtca tcgagatctc tcatagccac gaggccttcg cactgggagt
    12901 cgcttagctc aacaaatatt ccaaagttgg ttacccctga aataacaccc ataaacacct
    12961 gtccaacctt atcctgcatg aactcaacct gcttgtactt aatagaggcg cgctcggctt
    13021 cggtagccct aatctccatt tcgcttgcat gcttgcaaag caattcaatt tgctccttat
    13081 ccttaggctt accgttgcta aggtaatgcg ctaaaagcct gtgaaccatc atgtcggggt
    13141 aacgacgtat gggcgaggta aaatgggtgt agaaagggaa cgccagtccg tagtggccga
    13201 tgttgttggc agagtaacgc gcttttgcca ttgaacgcag tgcaagtgtt tcaataaggt
    13261 tttgctcggg ttttcctcgc acctcgtcca tcaggctatt aagtgattta ccgagctgcc
    13321 tgctgctctc ccctgaaagc ttgtacccga agcgggtaat aaatgtgcgg aagttatcga
    13381 gtttttcctg gttaggctta tcgtgaatac ggtaaacaaa aggaaatcgt tctttaccag
    13441 gtttttgctt gccaaccagc tcggcaacct ttcggttggc aagcagcatg aactcctcaa
    13501 taagctggtt cgattccttg ttctccttaa agtacactcc aagtggctta cccttggcgt
    13561 cgagttcgaa ttttacctcc atccgctcaa atgcaatggc gccattttta aagcgttcac
    13621 gccgaagctt ctgtgccagc ttgtggagaa gaagtatttg ttcatggagg tcgccctcgc
    13681 cggtttcgat aatagcttga gcctcctcgt aggtaaatcg tcgcttggag attattacag
    13741 tgcgtccaaa ccactcgcta aggacttcag cattgtcgtt taactcaaaa actgccgaaa
    13801 aacagagctt ctcctcgttt gggcgaaggg agcaaatgta gtttgaaagc ttctcgggaa
    13861 gcatgggaac gcaccggtca accaggtaaa cggaagttcc ccgttcaatg gcctcgttgt
    13921 ctatgggggt attcggcttc acgaagtggg taacatcggc aatatggaca cccacctcaa
    13981 tgttgccact tggtaaaatt tgaaccgata gcgcatcgtc aaaatccttg gcgtcaaacg
    14041 ggtcaatggt gaatgttggc acatccctaa aatcgcgcct gctggcatag tcggcattgg
    14101 aaatacggtc gtcaatcttc tcggcaagtt tctcgagttc ctcggggaac ctgtagggca
    14161 ggtcaaactc tgccagaata gcgtgcattt caacctcgtg cacacccgga tttccaagaa
    14221 tttcaactat ttttccattt gggttctttt cccggggagg ccaatcggta atttgcacaa
    14281 taaccttttg gccattttta atatccctaa gttcaacatg gggaataaag atatcatagg
    14341 gcatcagctt attgtcggga gccacgaagg cgtggttgcg aagcaacgat atggttccga
    14401 caaaatttcg cttggcacgc tcaatcaccc taataacctc gccttcaagt aaccccattc
    14461 gtcggtggcg gtaaatgcta atctccaccc tatcgccatg cagggcgtgg ttaagcctaa
    14521 aatcaggaat aaaaatctca cggttatcgt cggtggttac ctctcccgat ccatccttac
    14581 tcattaccag aattcctgtt gccaggcttg tctgctcctg taggcggaac ttgcccctcg
    14641 aaagctcaac cagccgttcc tcgccaacaa gctcgtagag cacctcggtt atcatcctcc
    14701 gggtgttgtc gttgttcacg ccaagccttg ttgatagctg cttgtagtta aaactctgat
    14761 tagggttttt gatgaatacg tccatcagac cctcgcgaag gtcccttttg gtaacccctc
    14821 ttttctgggg agttctcccc gagggctttt cgcctttttt gctcaccttt aaatgtttga
    14881 gttttatgct gatattagaa tttcaaatgt agctgttttt gcgctatcct gattttaaaa
    14941 ttttaaagag ccgtgatggc attatctttc cgtgaaaata aggacgtttt attttctatt
    15001 cagtattcag ctgaacgtac agccttatcg caggataatt gtcatgctta cctcacttca
    15061 tacatcagca atgaacacct gtttaaaaaa ccatcaataa aaaacacctc tttggtgaga
    15121 ggtgttgaat agcctttgta gaaaattgaa ggaattctta aactttggca aattcaataa
    15181 ttacttttac tgtcatatcc ccttcatctt cagtgaaagt ttcctgaaag tagccaacca
    15241 tcttaccgcc atccttagtt gtaagagtta gggtaacttc ttctccatct tgatttatgg
    15301 caagcttatc acccttaagc gtccatgtga atgtataatc ttcactatcg atgcctgttc
    15361 catcgctgtt aaattgtatc catcctagat cttgcgtttc tgtatnnnnn nnnnnnnnnn
    15421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15481 nnnnnnnnnn nnnnnnnnnn nnnnnatgcc tgttccatcg ctgttaaatt gtatccatcc
    15541 tagatcttgc gtttctgtat aggtatatac aggtgttccg gtagcgctag tacctgagta
    15601 aacatttgtg gtctctttca caatgttcca tttcccctca acagaaaagt cgtcatcgtc
    15661 actacatgcg gtaaacatta aagcaatagc cgcaacaatt gcaaataatc tcagttttct
    15721 cataagttat tggttttaga atttaaaaca aacgtacata ataattttca actatcaaac
    15781 aaatgttttg aaacctttgc ctggtaaata agaaaggcag atgaaaccga ttagcacaga
    15841 cttttaccta agctaataga tagtagcaag aatgtaagct aatcattttt cccaaccagg
    15901 aagcttaagt tcacaaaaga tatattctca tttttttaga aagcctataa gcaaacgatt
    15961 actcccaaac ttcaaaggaa caggtgggca tagaggatgg ctcatgaaag aaagattccg
    16021 aaagggcttt agacgaaaac cttccagcac accgcctata ttcatattta caggttagaa
    16081 aagggggtag ccgctcaaaa caaccttcat gtagaagaaa aggttgtaat aactattggt
    16141 atccagaaac accaacaagg gtcaggcgta aaaaaaatca gcatcacttt ttcatttttt
    16201 tatccggcca ttgtttgaat tttttttaca ttagctccgt ttatatcttc atcagcatga
    16261 tttcgaaaga gggtttaaaa gggttggttg atctacatat tcaggcaaca acggaaactc
    16321 cggaagtaat ttgtagcaaa agcacaggaa aaatttcgat ggttgggaat ttaataccca
    16381 gcgatcccga gtcgttcttt gctcctatca ccaggtggtt tcttttatac tctacggaat
    16441 ttgaacccaa gcaaattact gttgacctat acctcacatt tatcaacggt tgctcggagc
    16501 accatctggt aagtttctta aaaaaagttg aggagctata cagcaggggt gttgaagtaa
    16561 gaataaactg ccactacgaa agcgacgact tcgacatgcg cgattggggc cgcgacctac
    16621 gactagtcct caaaattccg gttgatatgg ttgagataaa ctagcagttt tcctctaccg
    16681 cacatctttt atagccattc cccgggttta atatttctaa taaactcaag agttttatca
    16741 atttccttga acataattct atcctcacca aaaaacttaa cctgccggcg gtagcttgcc
    16801 agtatgcttt cgatagccgg cgaggatttg gctggacggc gaaactccat tgcctgggct
    16861 gcgttcagga gttcaatggc caaaatgcgt tcaacattat ctaccacgcg cagcagcttg
    16921 gttgcagcgt tagcccccat gctcacgtgg tcctcctggc cgttggatga aacgatggaa
    16981 tcaaccgacg caggtgtaca aagctgctta ttctggctta ccatggcggc agcagcgtac
    17041 tgggggatca ttaaaccgga atttagcccg gggttagcca ccagaaattc aggtagcccc
    17101 cttaacccta aaatgagctg agctgtgcgg cgttcggaaa tgcttcccag ctcggcaagg
    17161 gcaatagcca gaaaatcgta ggccagggct agcggttgcc cgtggaagtt gccccccgaa
    17221 atgaccaaat cctcatcggg ccaaatggtg gggttatcgg taacggagtt aacctcggtg
    17281 agaattacgc tggcaacata cgcaatggca tcccttgatg ccccatgaac ctgagggata
    17341 cagcggaatg agtagggatc ctgaacatgc tttttggggc ggttaatcag ctcgctaccc
    17401 tccagaattg ccctaatgtg cctggcggtt tcaacctgtc cccgatgtgg cctggcctgt
    17461 tgaatattct cgtggaacgg ctctacccgt ccatcgaaag cttctaggga gattgcgcca
    17521 atcaggtcgg aaaggtttat caacctaaat gccttgagca ttgcggaaac accatgggcg
    17581 ctcataaact gggttccatt cagcaaagcc aacccctcct tcgacttcag ctcaatgggc
    17641 tgccacccaa actcctttaa cacatccaga gcctctacaa cctttccctt ataccttaca
    17701 tcacccatgc caagcagagg caaaaaaaga ttggccagcg gagccaagtc gccggaagct
    17761 ccaagcgagc cacgatcgta aacaacaggc agtacgtcgt tattgtagaa atcaatgata
    17821 cgctccaccg ttgcaacctg aacacccgag tttcccaacg acagggcatg tgctttaagg
    17881 aaaagcatta gtctaacaat ttccgaactt acctctgcgc ccgttgaaca ggcatgggac
    17941 atgaccaaat ttttctggag ctgcgacagc tcatttcggg atattgtttt gttgcaaagc
    18001 gagccgaacc cagtggtgat gccgtaaatg ggaacgctgc tttcctccat cttgacatcc
    18061 aggtaacggc ggcacttttc aatcagaact tttgactcat ccgaaagcga tagcctgaaa
    18121 tccccggtgg taatatcctc taaaaccttc catgtgaggg gttggggtga aatcctatga
    18181 attctggtca tggctttaaa tattttcttt gatatagctt acaacatctt cagcagaaat
    18241 agtagcctgc tctcctgttt ccatattctt cactgatagc tggttttgct gcatctcgct
    18301 ctcgccggca atgacaacca gcttaatcct acgccggttt gcatagtcga gctgcttttt
    18361 catcttggca gggtcgggat aaatttctgc acttacaccc tgctccctaa gctgccgaag
    18421 agctttaagg cagtatgcag cctcgctttt cccgaaattg gtaaacatta cctgcgtgga
    18481 ggatagcagg ctatcgggaa agaggttgag ctgcagcatt acgtcgaata tgcggtcggc
    18541 accgaacgac actccaaccc cgcttaggtt tttcattcca aaaataccgg caagatcgtc
    18601 gtagcgacca ccgccacaaa tactgccaat ttcgacatca gcagccttta cctcgaatat
    18661 ggttccggtg tagtagttta gcccacgtgc cagcgagagg tccagctcaa cttccgataa
    18721 aagctcaccg ctctgtcgaa tcagctcaag cagctcttcc atctcgccaa ttcccctcat
    18781 ccccacctcg gaaggagcta gtgtattttt caacagccca agtttttcgg tgttgttgcc
    18841 cttcagtaaa ataactggct gcaacttttc tatggcgact tcggatacac cccgttccct
    18901 gagctcgctg ttcaccccat caataccaat cttgtcaatc ttatcaatgg ccaccgtgat
    18961 atcggttaac ctttcggctt caccaataac ctcggcaata ccgcttagta ttttgcggtt
    19021 gttaagcttc acaaccacgc gaacacctaa ccgttgaaac acctcgttga tgatgagaac
    19081 cagctccagc tcgttgagaa gcgaggtgga tccaattaca tccacatcgc actgatagaa
    19141 ctccctgtag cgtccttttt gcggtctgtc ggccctccaa acaggctgta tctggtagcg
    19201 cttaaaggga aacacaatat cgttttgata ctgaactaca aagcgggcaa agggaactgt
    19261 taggtcgtag cgtagtccct tttcgcaaat ggcaagcgaa acggcattgg agttgccagg
    19321 tctggctgtg ctggggtcaa ccctttcaag aaaatcgccc gagttaagaa tcttaaaaag
    19381 tagcttatcg ccctcctcgc catacttccc cagcagggtg gagaggttct ccatggcagg
    19441 cgtttcgatg ggttggtagc cgtaaatact gaacaccgaa cggatggtgt cgaaaatgta
    19501 gttccgccgg accatctgtt ccggattaaa atctctggtc ccctttggaa ttgaaggctt
    19561 gctgcttgcc attgccatct agtgtttttc aagttttaat gatcagtaac cgggttaatg
    19621 ctccaacgtt agctacccgc tgtcgcttaa agcgcttacc tgttaatacc caagctgaaa
    19681 tttttcggca attggcgtat ccaggtattg gatatttagt aagcgactgc aaacctacta
    19741 tttttttgca acccgttgaa ggggttgtcc gacaatttta gcatatagca tgttacgccg
    19801 tatttagctg gttggccacg gctgtgcggc taccataacc ttaaggttga taggtaatca
    19861 tataaattat cggcaaattc ttcctacttt tacacctctt gcccttgctc cacaactatt
    19921 cctaattttg aaaacctgct taaatcaacc tcatgcagtt tggatatgaa cggccagctt
    19981 aaaagcctgc ttaggaaagc cctgaaccgg cttcacctcg atctcacaaa aaatttgagg
    20041 tacgacaggc ttagtaaaag gattatctcc ttacacctaa agcccgactc cacctgcatt
    20101 gacgtgggtg cccatagggg agaaattctt gacctgttcc tacaatttgc cccaaaaggc
    20161 catcacctgg cctttgagcc tattccttcg ctctacaatg ccctcgaaaa aaaatactcg
    20221 ggaaggtgcc gggtatatcc gtttgtgctg ggagacagga tagggaaaac ccccttccag
    20281 ctggttagaa atgcgcctgc ctacagcgga attttaaaac gaagctacag catacgacat
    20341 cccgaggttg aggagattga ggttgaaatg gccacgctgg actccgttgt caatgaggag
    20401 gaaaatatag attttatgaa aatcgatgtc gagggtgctg aactccaagt gctacttggt
    20461 gcccgtagca ctctttgccg ttgcaggccg aatattattt ttgaatgcgg gctgggtgca
    20521 agcgactact acgggactaa ccccgatgat ctttacgatt tcctaaccgg catcgtaggt
    20581 ctgaaaattt caacccttaa gggttggctt accgatagcg gaaacccgct aaccagggat
    20641 gagttccgcc gccattttga gcgtaattca gagtactact tttttgctca cccttagaat
    20701 agctttaaat attaattttt agagtatcat aacctacccg taaagaagat ttcaaaatgt
    20761 tgaaagtcgc aaaaacacca tttaacccga atttaaaggg cgataaattg cggtataact
    20821 tttgaacaac ttgtcctgct ccatttaaag gataccttga gaagtgtaac ttttaagcaa
    20881 ctccatcgtc ttacatgaaa tttacagcca tagatgaccg ttgaggattt taacagctgc
    20941 gtagatttat actccgacgg aatatatcgc tttgcattga aaatgcttgg cgacaccatg
    21001 ctggcagagg ataacgtgca ggattctttc gagaggctat gggttaagcg tggggaaatt
    21061 gataggtcaa aagcaaagtc ataccttttc accacttcgt accatgcctc ggttgacgcc
    21121 atcaggaggg ataggcacaa gcaggagcta gaaaatgcgc agccagtatt agaaattggc
    21181 tatcaaccca attttgacgt aaaggaaatt atcaaccatg cggtggacca gcttccaacg
    21241 gttcaaaaat ccgtgctgct gctccgcgac tacgaggggt actcctacgg tgaaattgca
    21301 gggataacag gactgaacga atcgcaggtt aaggtttaca tctacagagc cagagttttc
    21361 ctaaaggaaa aactcgtaag tattgaaaat ttaatttaac tccgatgagc atcaacctaa
    21421 atacatacga ggagtacctg ctcgactaca tcgatggcaa gctttgcccg gaggttgagg
    21481 ctgaactagt aaagtttctt cgcttacatc ccgacattgc ttcagaactt gatggattaa
    21541 accagctaac cattccaaaa ccaaaccttt ccttttcagg gaaacaacgg cttaggagaa
    21601 ccgttgaaca ggatctcttc tcatgcaacg agatggacta cctttgcatc gccgaactgg
    21661 agggtgatat ttccgaggtt gaaaaagcaa ggctcgaaga gctgaaaggg cagcatcccg
    21721 aggttgaacg gttccgcaac ctttatgccc ataccctttt actttccgag aatagccata
    21781 cttttccaaa caaagcttcg ctaaaaaggc atagtatcat ttcaaaacga aggattatgc
    21841 taacagcggc agcagcgatt actgcagcca ttgttggcct tcataccatc cacaagctaa
    21901 gccttcccga cgatttgcaa ccccaaaatc aaatggccaa aagcgcaatc atagaatcgc
    21961 agccaaggga gaacaggcca acctccacca ccacttaccc acaaacccag ccaaaaccgg
    22021 agagccgggt agcagtgtcg ctggcatata aaacctctac atcaacaata agcatggaca
    22081 acgaagagct tcagcccatg agaacaaatg tggcagagct aaagcaaata gcctccaaag
    22141 agccgataat tgagccaatt gctagtatta cccctaaacc ccttccaata actggcaagg
    22201 tacaaggtgt tgcttttcgg gaaaaagaat tagcacctaa tgtagcccca tcaactgcca
    22261 aagtttacac gctggccgat attgctggcc tgagtttaaa acgtcttgca gggttggttg
    22321 gcatcagcta taacgtaaaa accgacagca ccaacacctc caagcatatt gaggttgact
    22381 ccaggctaat agccatctcc accataattc gaaaaaataa ctagcggtgc tgtaacaaaa
    22441 cgacctatcc taccgtccta cagaaaattt taatcaacct tctaacttca actaaaatga
    22501 aacagctagt aattcttgca tcatcgctgc tgagcttttt caccctaacc gcacaaaacg
    22561 aaaaggaaac catgctccgc agcctcgaaa tcgctgacac tttaaaagtc tctgataccc
    22621 tcagcacaaa tcataggaaa cccaacatgg tggttaatgt ggagaaaacc attaaagtag
    22681 ctgatggcga tacggttgag gagactacct ccgacacact tgttgttgat agcaataccg
    22741 gggacaataa tgttgcttac caaggcgaca cgataatagt ggacagaaac caaggcaaca
    22801 cgaaatcgga ctctaccata atcaggcttg gcgaaaaaac catcgtgatt gttgagggta
    22861 aggataaaac taccgttgaa atccccgagg gtctgaacaa ggattttagg aactcgttca
    22921 ggcgtagcaa ccgactaaaa tttaagggtc attgggctgg aattgaaatg ggtgtaaatg
    22981 gctttatgga tgccgaccac tccacctcga tgagcaacga gcttgaatgg ctcgacctga
    23041 agcaaagccg ttcgtggaac gtaaacctaa attttctaca gtacagcatg ggttttggaa
    23101 ccagctggat tggattggta accggtatgg ggcttgaatt taacgactac cattttaata
    23161 accccttgac tctaaaggtt gtaaacggag taacggttgc cgacagctcc tacctgcttg
    23221 ccggatacaa ggttgataaa acaaaactaa gcacagttaa cctgaccctt cctttgctag
    23281 tagagtttca gatacccact agcgaatccg atcgccacaa aatttatatt tcggcgggag
    23341 taattggtgg gttgaggatt ggcacccaca ccaaggtagt tttcgacgat ggcgatcgcc
    23401 ataaggataa ggggcgtagc gacttcaaca ttgcaacctt ccgttacggc gttactgcca
    23461 gagcaggata caggagttta cggctttttg ccaactacta cccaacccaa ctttttgaaa
    23521 agaacaaagg cccggaaatt tacccgtttg cggtaggact ggtgcttata ccattctagt
    23581 gtaagctgat agaatataga atgcccatcg taataccaaa cgtttcggac agcgtataaa
    23641 ggttgggcta aagtggctgc ggccggggct ttgccccggc cgctttcctt ttaccacctc
    23701 aaactcttcc tcccttttcc cattacatct tacttacagg aaaagtaaga ctgaaaaaat
    23761 acagcagatg cttctctttt ccattccagc atttaatccg gtaaacgtca acttcatgat
    23821 taggttgatt gggttgaata aactgtctct ttcaataagg cattatcatg aacaggctaa
    23881 atgcagctct ttgcagatgc gtaagaattc aactaacctc cccttctaac ttaaagctgc
    23941 cgctcctcct ccacgaactt taccccgtac tcctcaagct cattcagaat ggggttgtag
    24001 aggtggggtt taatgggcat caccacaccc ctgtcattca cctgcccggt cattagcatt
    24061 ttggcagcta tggccagggg tgtgcccacc gttttggcca tggcggtatg aacggtatcc
    24121 tcgccaacga gcgtaagagt tgatttcttc tgaaacttct tcccgtcaag ctcatagtcg
    24181 aatatatgct gcatgatgag gatatcctta tcgttgggtt caagaccaag tttttcctcc
    24241 ataattctct gcagcagctg ggcggggctt gcgttggcca tgccaataag ctcgttgtca
    24301 aaaatgccca gccattttag cttgttaaaa ataatcttat cctgagcagc aggaatcacc
    24361 cgctgcagtt tctcctcaac ggtttcggta ggattatagg gtagaaaact gttgagaaac
    24421 tcccttttgg tcaacatctc gctgccctcc atcttgtagg tatcgtcggt cattcccagc
    24481 tggacaaaca catcccaggc tttgctgtag cccgaccggc gtagcgttcc gcgtataatg
    24541 gtttgaatat cgttaatacc gtatatttcc ctgtacttaa gagagtcgcg atttgggtac
    24601 atttcaaact cgccataggg aggaatgttg taggtgcgaa tgttggagta aagcctgtgg
    24661 tatggaatgt acttatgctc tccattctca aggaaatgcg taaccccttg tcctgcaagc
    24721 actacgttgc gtgggttcca ggtaaacttg tagttccatg ggttgttgtc gcactcagga
    24781 gcaatcaacc ctccggtgtt gctgatgaat ccggtaattt tccctccctt gttcctaata
    24841 ccgtcaatca tcctcatggc cgacatgtgg tcaattccgg ggtcaacccc cagctcgttc
    24901 atcaaggcaa cccctttttg tctggcctca tcgtggagcg atttcatggc atcggaaacg
    24961 tacgatgcgg ttagcaggtg cttcccctgt cggacgcact ccttagccac cactgggtgc
    25021 atgaatgcag gcagcatgga tattgccaca tcggcccagg ccacctcagc gctgagctgt
    25081 tcagcattaa aaatatcgaa cttgaaggcc tgtccatttg gatttccccc aagcttaccg
    25141 gcggctgctt ccaacgaaag atcaccaata cgaattgtcc agccataggc tggcgcattc
    25201 cccaataggt actcaataag gttggtggac gaaagcccgg cacccagtat caaaattcta
    25261 ctcatctgca tggcttgtat gttgttttag ttactattta ctaaaccatt tatcacctag
    25321 cattcaaacg atcaagctac agacctaacc ggttcgctgc ccttgtatgc aaaccggctg
    25381 atgctaaaat agtaaaacca gccccgcttg ctttcagctt taaagtttac cgctaaatat
    25441 cctgccaatc taaaacattt tttgggaata tttgaattat ttttttaaaa ttcacaccgt
    25501 ttgttaataa caagatttcc tcaactttat acatgaatag attactgtat aatcatttca
    25561 gtcagtaaga aattaccata gagcaaggtt ttttacatgt tctaaatcgc tatctttgac
    25621 caaatggtgc cgaaatgctt accctgtcga ttggttttgg cgagcttgtg gttatcctgc
    25681 tggttaccct gctggtggtt ggccctcgtg aggtagcaaa ggttgccagg aagctgggaa
    25741 aactttaccg aaggatggat aacgccatgt ccgatttacg cagctacgtt gaaaatccct
    25801 ccagcccggg tgataacagc gatttggtag atacaagtga aaaaaggaac gattcggata
    25861 atgcaaacaa aacaccggca ggataagtgc caccacaacg ttactttggg ctaaattggt
    25921 acatttgcgc aggcaaaaat ctaatggata gcaatgataa aggtcgaaaa tattaccaag
    25981 tcctacggtg cactgatggt tctaaaaggt atcacccttg aaatacctga gaaaaaagtt
    26041 gtagcaattg ttggccctag tggcgccggt aaaacaaccc tactacaaat aatgggtacc
    26101 ctgggcagag ctgattccgg acaagtgctt attaatggcg aaaatgtagc acagctgaac
    26161 gaccgccagc tggccaaatt taggaatacc catattggtt ttgttttcca gttccaccat
    26221 cttttacccg aattcactgc cttagaaaac gtttgtatgc ccgccctgat tgcaggaaaa
    26281 ggtatggcaa cggccacaaa aaagtccacc gagctgctca agatgcttgg cctatcggac
    26341 aggctcgacc ataagccctc ggagctgtcg ggaggcgagc agcaacgcgt ggccgtggcc
    26401 agagcactgg ttaacaatcc tacggtaatt ctggccgatg aaccctccgg taacctggac
    26461 tcccgaaata aggaggagct gcacaaactt ttcttcatgc ttcgcgacca gctgggccaa
    26521 acctttgtgg tggttactca cgataaggat cttgcccaaa tggctgacct gcaggttggc
    26581 atgaacgacg gactgctttg cccatgaaac ttccacaaaa ccgtgcagag ctgacacaac
    26641 tactcaacaa ccgctatcat cgctatgccg gagcatcctt cctttccgac gaccctatac
    26701 ttatacccca ccgttttact aaaaccgagg acattgagat atctgccctc ctcaccgcca
    26761 ccatcgcatg gggcacccgt aaaagcataa taggcagcat gaacaggctt atggccataa
    26821 tggacaacag cccgttcgag ttcgtgataa atttctcaga aaacgatgag aaacggctaa
    26881 acggctttgc ccatcgaacc ttcaactcat ccgactgcat tgcgtttatt agaagtttgc
    26941 agcgcatcta cgccgatggt ggaggcttaa aagccatctt taccaatggc ttccgacaaa
    27001 caagaacagc caagcaagcg ttggaggcat tccggctccg gttaattcag gaaaatttta
    27061 gcggtcatgc catgaagcat gttccaaacg tggagtcggg ttcggcagcc aaacgcctca
    27121 acatgttcct gcggtggatg gttaggccgc caaaagaggg gatagacttt ggcctctgga
    27181 gcgaaatgcc cacgtcagcc ctacttatcc cgctcgatgt tcatagcggc agggttgccc
    27241 gagcactggg gctgcttacc cgcaaacaaa acgattttaa ggctgtagag gaactcactg
    27301 aatccctgcg tggatttgac gctgacgacc ccgtgaagta cgactttgcg cttttcggca
    27361 tgggggtcca cggcgaaatc taaaccacat agatgttctg aaatggcaaa cagctacttt
    27421 aggtttaagc agtttaaagt gttccaggaa aaatcggcca tgcgggttaa caccgatggg
    27481 gtgctactgg gggcatgggc agatgtctcg ggggtccaaa gaattctgga tgtgggcacc
    27541 ggcacagggg ttgttgcgct catggtagcc cagcgcaacc caacagcaat ggttgacgcc
    27601 gtggagattg acgcaccatc ggccgaggag gcagcaggca acgccgcccg atcgccctgg
    27661 gccaacagga taagggtagt ttgcacctcg tttcaggatt ttgcctccag cgctgaattc
    27721 cgatacgacc ttattgtctc caacccgccc tacttcaacc gctcgcttaa gccctccgac
    27781 ggcagaagaa ccataacccg ccataccgac accctgccta cccctgccct gcttgaggga
    27841 gctacaaagc tgatgctgcc aggtggacgg ttttgtggaa ttttccccta cgccgagggc
    27901 aacgttttta ttgctcaggc atcacactat ggtctgttct gctcgaaaaa acttaacata
    27961 ctgtccaagc ctggtggcag aacattgcga atgctggttc agctggaggc tgaaagaaag
    28021 gccaccgtag agtcatccct ctgcattcac acggttgatg gatcgttctc aaaagaatac
    28081 aggaatctta cctcggagtt ctacatggac ttttagcagc ccttgccact cggattttct
    28141 gaatgagtat agctgtgtgg aaattcccct ggcataagga gagaactccg gtttataaaa
    28201 ccaagtagcc ttttaggctg atttaaaaag ccaaatatgt tgggcaggat accactactg
    28261 ccccgaagct gcaaaggggt gctcccgaaa gggcctatgt acggtaaagt tttttttcat
    28321 cgatctagta aaccaaaagg aatatttcat cagtatacag cattaatgca atggctgaca
    28381 ttatgttacg aattggcgta tttacactgg ttaacgttcg gatgaacccc gaagtgctcg
    28441 ccgtaggcaa atacctattg ctcattttca cgtttctgtg caacttgcct tacttggtag
    28501 tttattaatg agttagcaca taaaacctta aggttggcaa gctcagtaca agaaaatagc
    28561 tatgcactac ttatcgtagt ggtatctgca gctgtaaatc aatatttcct ggtctataac
    28621 ccggtaaacc aggcgatgct ccctatcaat ccgcctggac caaaaacccg ccagatcgaa
    28681 tctcagaggt tcaggtttgc ctaacccctc gtaaggatgt ctttctatgt ccttaataag
    28741 ttcgtttatc tttttaagta taagcctgtc atcaacctgc cacgacttgt actcctccca
    28801 cgaatttctg gagaaggtaa ttctagtcat tactctaaaa gtttgccttt taccactttc
    28861 cgttcgttca tctggttaat ggattcaaag agtctttccg cattagcctt tgaactcagc
    28921 aagtgtaagg tttccattat ggaattatac tcttcaagcg acatcatcac ggcacccatt
    28981 cctgctcccc tcttaattat aagagtttca ttgttcatct caacttcgtc caggaacttt
    29041 ttcaggccta ccctaaattc agtaaaattt gctgcgagca taatctaaaa attttaggtt
    29101 actatttgag tacaaatata tgtacttata ctgaattttt ttggattaag ttttaaattc
    29161 gttcaccgat ttccgataaa taaattaaaa tatggattta cttcccgaag gtttcacccc
    29221 atgcttcaag catgttggcc aactagcttc aacatagtta aaaaccgcat atgtttttca
    29281 gtattttaaa aaaacaatgt gaagcttatc gctatatgaa gcacccattt tttgtgaact
    29341 ttatggttaa ataatgttag aaaccacaaa gcacacaaag acattacaca aagaacgcaa
    29401 agattactat tcattattcc gatatggtta tcggaaaaca acattttctt taaatggaga
    29461 caattttatt atcgtgaaaa ttgatggagt agctgaatta atattgttcc attaacattc
    29521 aggttttatc cgcacaattt tcaaccatta ctcatccacc ttctgaaaat gcttgttata
    29581 aatatctatt aagaaaagca aaaccgatat acctaaaaac aaaactttag ctaaagtagg
    29641 gacatctaaa aatatcaaca atattaaatt gataaaagaa gatgtacgta tgaccacatc
    29701 aaaacttcct gatgaaatgg actttcttaa aatgataagt cttagggatg agaacacccc
    29761 tgcaattcca attataaata taaagtttct gtcatcaatg ccttcataaa taacagacat
    29821 aacagtatat cccaaaaaaa tcaataaaca taaagtgcag gtaaaatagt cgataaaaga
    29881 atactccttg ctcgtaaatt ttgtaatggc aatcgtattt tcaaagttgt cgaactgata
    29941 tttgtcaact gctttaaccc tcatgcagtt cctgaacatt acatcgagat ggctaaaagg
    30001 aaatttatcc ctaaattcta gcataacaac caggtagagt ttctgatcat tcgtattcct
    30061 atcaatagta aacctgtgct tatggtaatc cagcctgaag tccaaccatt cgtcatttcc
    30121 ggaattaatt gttataacct cctcatgacc aacatagtat gtgccaccag agctggtctt
    30181 gaccttaatt cgtcttaacg aatagtagct atttttaaaa acgcacttaa tgtaaatggt
    30241 catcatccgc tacctgttaa caaatcatta tatatattca ctttgaagat acttcaccaa
    30301 aatatttaca ctgctgatga aaatatcgca caaccatttc aaaaaaaacc tgcatccatt
    30361 tgtccaagta ccagacaatt tttcgggata aataaaagta cctgcaatat agcaaatttt
    30421 ccatatcaat aacttgaaca ttggccaaca ggcatcttca agtaaacggc aactttatcc
    30481 acagtacaaa ttccttcaat gttgctattc aagcgcttac cgcctatata cgccaacatt
    30541 aagcgttaaa cctctccaag ttatggataa atgtacattt aagtgtctgc cttcaaactc
    30601 taccactata aatgttggta agcatttggt taacgtcatg attagttgca tttggaccca
    30661 aatcaaggca gcctgtaaca agcatttttc gcgtaattaa gccggcatat ttagaaaact
    30721 tgcttataag ttcaatgctt gcataggtac agcaaaccta acaacaaata gcatggctac
    30781 tcaacttttc ctaaaccggt ttccagctgt actaccattc atttattttt ttggaataaa
    30841 tttcctttgt aataattatt tttatacatt tgcaacctca aatttttgag aaacaggcat
    30901 tcatcaactt gaaaactagt tggatgcaaa caacgcaagg ttaaagagtt aacctttcag
    30961 gtgtaacagt aaatagtaaa atggaaaaat caagttttaa cagggatgtt aaacgcgaga
    31021 agcggactaa ctcccaggaa gaacccacca gggtttaccg gccaaggact ttgacatcaa
    31081 gaaaagccac tccggaaacg gaaaatgaaa acagcacccc atcatccgag ggcgaaccca
    31141 aaaagttcag ggatgattac gacaactccg ggcagcactc cgagcagcag aacgaagaac
    31201 caacccaaca acgcagaaag ctaaaaccaa tcgcagcacc tgctggggat tttccatcac
    31261 ggccacatcg gccgagatac gacgaccatg ttggcgagga gcaacctaat cgctacaccc
    31321 cgcgatctgg taaatactct gaaaccgatc cctcaactcg ctttagcaga aaaccatacg
    31381 aggatagccg tcctgatgtt cataagccat tcaggagttc agaccgctac cacgatgata
    31441 aacctaaaca tttcgagggt aacaggcacg aattttctga cggacacttc tcccacccca
    31501 aaaacttcag aaccaacagc cgtaactttt catccgatag gagggaaagg cagggctcca
    31561 gctacaacag ggatttcgat agcaatcctg ctcaaagcta ccgttcaaga acatttcaag
    31621 gcagctacgg agatgagcgc aagcacatag aacgtaaaaa tcttaatacc tttagggaga
    31681 gaagccccca aaaagataat tccggcgaaa tttccaccaa tcctactggc cccatcaggc
    31741 ttaaccgcta tattgccatt tcgggattat gcagccgacg ggaagccgat gtttacattc
    31801 aaaacggaca ggttaccgtt aacggcaagg tggtaaccga gctgggcacc aaggtgaacc
    31861 taaccgatga gatttgcttt aagggtaagg ttctgaaatc ggagcgcatg gtgtacatcc
    31921 tgctcaacaa acccaaagat tacatcacca caatggatga cccacacgcc aagcgtacgg
    31981 taatggagct tattgaagga gcctgtcctc aaagggttta cccggttgga cgtctcgaca
    32041 ggaacagcac cggcgtgctc ctgctaacca acgatggcga acttaccgca cagctcaccc
    32101 acccaagctt taaaaagaaa aaggtgtacc aggttggcct cgaccgtaag tttaacccca
    32161 acgattttca aaaactaacc gaaggcattg aaattgacgg tgaagtggta aaggttgacg
    32221 ccgttgacta cgttgaggga ggcgatggca ccgagctagg agttgaaatt cacagcggac
    32281 agaaccgggt tgttcggcgc atgttcgacg ctttgggcta cggggtagtt aagctggaca
    32341 gggtttactt tgccggcctt accaaaaaga atctaccacg cggtaaatgg cgtttcctta
    32401 cgccaaagga agttaacatg ctgaaaatgg ggaggttcta accccccctt cctattcaga
    32461 aaagtagcct ttactacctt acacgttaac gtgagccaaa acactcataa aatcaggaat
    32521 tgctcatgac acacaagtcg ggctttgtaa atattattgg taatccaaat gtcggaaaat
    32581 caaccctgat gaatgcgctt gtgggtgaga agctatccat tattactagt aaagcccaaa
    32641 caaccaggca taggttgatg ggaattgtta acggcgaaga ctttcagatt gtttactccg
    32701 ataccccagg aatactaaaa cctgcctaca agcttcagca ggccatgatg aaattcgtgg
    32761 aaacagccct tgaggatgcc gatatcattc tctacgtaac cgataccgtt gaaactccga
    32821 ataagaatca ggagtttttg aacaagctgc agcaacttgg caaaccgatt gtccttgcaa
    32881 tcaacaaggt tgaccttact acccaggaaa aggttcagga gcttttcaac ctctggagca
    32941 ccatgatgcc caacgccacc gtcatcccca tatccgccct gcatggcttt aacctcaact
    33001 cgctggtgga cttcattctc gacaggttgc ccgaaggtcc cgaattcttt ccaaaggata
    33061 ccctcaccga caaaacctta aggttcttcg cctcagagat tatcagggaa aaaattctta
    33121 acaactaccg caaagaaatt ccctactccg ttgaggtgga aattgagcag tacaccgaga
    33181 gcgataccat tgaccgtatt aaggccatta tatacgtgga acgcgagtca caaaaatcaa
    33241 tacttatcgg tcatcaggga aacatgctca aaaaggttgg aaccgaggca aggctggagc
    33301 tggaggcttt cctaggaaaa aaggtttacc ttgagttgtt tgtcaaggta aacgagaact
    33361 ggcgcaacga tacgagcaag ctgaaaagat ttggctatcc cttatagcta ggttttaccc
    33421 atcataacaa gctttcccga accttaactc accctgcaga taagaagttt tgctaaacca
    33481 aaacataatt aacgtaaaat tgaacgttca gtgggaaata tagtagccat agttggtcgc
    33541 ccgaacgtgg gcaagtcaac cttttttaac cgtatggtgg gctccaggca tgcaattgtt
    33601 gaccaggtgt cgggcgtaac ccgcgacagg atttacggca agtcggattg gaatggggta
    33661 gaattttcga taatcgacac aggaggttac gtggttaact ccgatgatgt ttttgaggag
    33721 gaaattcgca agcaggtgat attggccatc gaggaggccg atgtaattct gtttctggtt
    33781 gatgtgcgcg atggaataac cagcatggat accgctgttg ccgacattct ccgtcgttct
    33841 tcaaaaaccg ttcacctggt tgtcaacaag gttgacaata acctgttgag gtatgatgcc
    33901 aacgagtttt acagacttgg gctggggacc cccatgtgcg tttcggcaat caatggcgcc
    33961 ggaacaggtg atttgctcga cgaggtggta aaaagttttt ctccggttca tgaggaggtt
    34021 caggagttgg aaataccgcg aatagcagtt gttggtcggc caaatgtggg caaatcttcg
    34081 cttattaact ccttggttgg cgaggaaaga aacattgtta cccctgttgc cggcaccacc
    34141 cgcgatgcca tcgatactac ctacaacaag tttggctata acttcactat tgttgacacg
    34201 gcaggcctgc gccgacggac gaaggttgag gaaaacctgg agttctactc ggttgttcga
    34261 tccatcaggg caattgaaaa ctccgatgtt tgcctgctcc tggttgacgc cactcagggt
    34321 atggaaaatc aagacctgtt catattcaac ctcattcaga agaaccgcaa gggtttggtg
    34381 attgtggtga acaaatggga cctggtggaa aaggacacca aaacaacaga tcggtacacc
    34441 aagctaatta aagagcggat agccccgttc caggacgtcc ccattgtttt tgcctcagcc
    34501 ctcaccaaac aaagaatact taaggtgctc gaaacggcat caaatgttta taaaaatcgt
    34561 aaacagcgca ttccaacttc aaaacttaac gaattccttc aggcaactct cgacattcag
    34621 cagccaccga cctacaaggg caagtacatc aaaataaagt atgttaccca gctgcctacg
    34681 ccaactccag cttttgtatt cttttgtaac cttccccagt acatccgcga accctacaag
    34741 cgatttatag aaaacaggct tcgcgaacag tttaatttca ccggtgttcc cctgcaaatc
    34801 tacttcagag caaagtaagc cttgcggagt tatagggata gccgaagaag gaatatctag
    34861 gaaacctgcc gccaatgcgg cctgtaaatt ttggatagca gcgatagctt gtaaaggtca
    34921 aacagctgaa tgttggggtt gctgctgcac agctgcctgc ggatgtaggg tgtgagcacc
    34981 ttaaaaaccc atcttaatgg gtaaagcatg cctatgtggc gcaaaaacct aaatatcctg
    35041 agcagcttta tggatttttc aatttcccta cggttccctg ctgaaatatt aagtatccta
    35101 accaggttaa ctattccctg ttccgttttg ctgataaact cgccgcatgg atcgagcccc
    35161 aggtgccgag cagcgttatc gagatgaaga atttcaatgt tatgcctttt gagctcaatg
    35221 cccagcagcg tgtcctcatg tccgtagccc gacaacgcct cgttgaacct tatggcgtta
    35281 aaaatatcag ctgatattac aaaatttccg ctaataaatg agctgtacgg atcggtttgc
    35341 cgaacaaagg ccgatttgca ctccctgctg acgccatact tccaccggag aagaagttcc
    35401 ggtctgaatg gcttggggcc atactcccga ccaccacaaa gaacctttgc cctgccaagg
    35461 ttatcaatat aggtttttag gtaatcgggg ctatcaacca taacatcgca atcgagaaat
    35521 aacagccagt ctccgtgggc gaagtccgcc aaccgattcc ttattttcga cctgcctatg
    35581 ttgaaatcga attccaaata gctggttttg ctacgagagg ccagcagtga attctgccgg
    35641 cggaagtact gctgcgaatg gtcgtcgaaa attaacacct ccaccgggta gttgagatgg
    35701 tttgcctgtc ggagcagctc atcaaccagc tcggttacat caaaattata taccggaatg
    35761 caaatggaaa tcatgatcgt ttagcttaac cagcaacacc ataattttga acgcaattaa
    35821 taaaaaaata aggagagttg gggtaccaat tccaataatt cggctaaatt tctttctata
    35881 cctaaggaca gtaaattcta ttgttaacag cttatgtatg cttgttttga ccaaatgtcg
    35941 catcctccat atcaaaagag cagtgcacaa aagtcactgg cacagcactt caaaatgaat
    36001 ggcaaaatgg agcagaaagc aattcggata tatcaatgta aattgatggt tcacggaaca
    36061 aaacaccttt taggtaaaaa attatcttaa atacttaatt tttagggcta gatttctgtg
    36121 ttttatttaa aacctatagt ggagtatggt taaattttac ctatcaagca gcctggtgct
    36181 gattttcaac atggtaattc cattttcagg aaggagtatt cttgcccagg actggaataa
    36241 gtttgaggga ttattcattc aggacaccac gcagaaggtt aagggcactt caaattgtgc
    36301 atttaaaaat atcagcaccc ttagctacta ccccgacacc cttcccggat ggttttttag
    36361 ccctcctgtt ctgtaccaag gagctgcggg tttgggaata tccgatcccg acatgccaat
    36421 ggagaaagca agagagcagg cttacctcag ggcaaaatca atggcactgt taaattacaa
    36481 cgccagggtt cagtactaca gggatatata ctcatcggcc aacgaggtgg gcaggtacac
    36541 caatcttcgc caacggttcg atacatactt caaaatttca tcgttggcca cggttagtaa
    36601 cgccatgttt gaggtagttg atacccattt cacccgttac aacgagtatg tggtattgct
    36661 aagctacaac cccgggaacc attcggatga tagcctttac aagcttacaa gcgttggctc
    36721 tgcattcctg gttgaggcaa cctttgatga cgtagttgac gaacaggcag agtatgagct
    36781 tctctgcaat gcaaccttcg gagaggactc cgcagctagc tcccatttcc ttttccggga
    36841 aaagggcaaa agatttttaa ccagctcctt catgaacaat gcagaggtag gttaccccat
    36901 gtttccctac tcttatgtat ctcctcagga ctccgcccgg caacacccct ttatttccta
    36961 ctctggcctt tggagcagct acctgaggca acttgttaac tatttaaccc ttaacgccca
    37021 gcagagcagc attaaactaa aaagcatgga cgaccagtac agcaccaagc ttaccaacct
    37081 tagcagggag attgcctcct tcagggcttc gctcaacata aacggcatta aatttgctaa
    37141 tgatacacta aaacttgatt tgcagcttaa tgaagtcgaa tcaaattttt agctttgatt
    37201 ttcacctttc ggattaaaaa tttttaaagt aaattgtatc atattaacta cctgttgtga
    37261 tgaaaaaatt taacctctac atgggagcta tcgtagccgg agttctcatg ttctactcgc
    37321 cggctcgctc gcaggagttc agcaaaattg tcccaaagca gctgggtgag aaaataaccc
    37381 tggagtacgc catttccggc gaaacccctg gtcagctatt taacattacc ccatactact
    37441 ccgttgatgg gggcaaaacc ttcaagcagc ccaaaagtgt caagggtaac gtcggtagca
    37501 atgtccccgg tggaagaaac ctcattgtgg tttgggatgt tcttaaggat ttggaagagc
    37561 tcaactccga tgtgattttt aagcttgatg caaaaaccag caccaccaaa ccactgaccg
    37621 acgacttcaa tcaggtacac ttctcgcttg aaagtctgaa ccgcaccaag gatggtaaca
    37681 tcgagctact actctcaatt accaacaacg gaccaacccg cgaccttaaa attatcaatg
    37741 ggcttgttac cattaccgac ttcaacaagc aaaagtacga cgcccaaaaa gggaacattg
    37801 ctgaggtaca gggcaacgag cggtactcaa ccccaacccg tacaatcaaa cagggcgaaa
    37861 cggtaaaatc gcgttttgtt tttgagcgca ttcctgccga aatggatagg attatgagac
    37921 tcgacctggg cgttgagctt attaccgacg aaaagtatgg aatagactta aagattggaa
    37981 agctccagtt ccgcgacctc cccgtatcgc cagccccaac cacaggcctt aaggttaagc
    38041 gggattacag ctttcagctg acctcccaac cggcacgttt cgatattcgt aaggttgaaa
    38101 ttgccgatgc tattcctcca gaaattgtgg ttacctcccc cgcagatgtt ccgttggccg
    38161 gcaaggaagc cacccggggt cgtccgtatg cccagtcatc cattggaatg gatgaccgac
    38221 ggcttagatc attggcttca ggtgaagagt atgccaccac cgacagcagc atgaccgtaa
    38281 agggcaaggc atcggatgaa agcggaattt accaggtgct ggttaacggt tttgaggcaa
    38341 aggttgactc caacggaatt ttcgaggcta gcatacccct ggaaattggc cgcaacgaaa
    38401 ttgccatacg cgcggttgat ctcagacaga atgccgttga aaagaaattt ttcgttctac
    38461 ggaaagcccc ttcaggaaag gtagccaagg gagaaaccga agaacttgac ttggtttttg
    38521 accaaccaaa gactgcacct aagttctacg ccttgattat gggcgcaaac gactaccagg
    38581 acaacagcat taccgacctg gataatccta ttagtgatgc cactaaactt tacaacgtgc
    38641 tggtaaaccg ctacaccttc gatcccaaca acgttgtttt ccttaaagac ccaacgcgtg
    38701 agcagatgat taatgccctt gatatgctca ccaggcgaat tggtaagaat gacaacctgc
    38761 taattttcta cgccggacac ggatactggg acaaggaaac tgattttggc tactggatac
    38821 ccacggattc aaaggggaat agcacggcca actggctggc caacagccaa ataaaagatt
    38881 acgttgcagc tataaaatcg aaacacaccc tccttattgc agatgcctgc tttggtggta
    38941 gcattttcca tagccgcaag gcgtttcagg agggttctag ctcaaccaag gaagcttttg
    39001 atgcgcccag ccgtaaggct atgaccagcg gaaaccttac cgaagttccc gacaggagcg
    39061 tgttcctcga acagctggtt acccgacttg atggaaacac caagaactac gtaactgccg
    39121 aggaaatttt tgccagcata aagaatgtcg tcatgagcag cagtccggta acaccggtat
    39181 atggagaaat aaaggatgca ggtgacgaag gtggtgattt tatatttgta agaaagtaag
    39241 catcagggct acccgaacat ggtgtagccc ttaatgtttt ttaccgaaac tctaaacata
    39301 aaatttcgta gttatagctg gtaaacattt accgaacact agcaaaattt tactcctatg
    39361 aagaaaatac tactcgcttg tttggctgct gcagtattct taataacctg cattgcagca
    39421 tctgcacagg aagatttaaa actccagaag ctggaggaaa aagtcaagat tgcccaggaa
    39481 aaactcgata aggccgatgc aaaggtcaac tctgctgaca gcctgattag cgcgggtaag
    39541 agcatcatcc aggaggccga ggttgaggtt agagctcttg aagctgaaaa gaaacaactc
    39601 gataaggccc attttgccga gcttaaaccc cttgaaaaac agcttcgatc aaaggataag
    39661 gacgaggcaa atagcgccaa ggaggaaatt aaagcttcag aaaccaagta taaggaagac
    39721 ctgaaaggct ggaatgcgag ctacaaggaa gctttaaaaa agtatgaagc tggaaagaag
    39781 atggttcaga agggggaaga aaacatgaag aaggcaaagg atgctcgaaa ggaagcagaa
    39841 gcaaccctaa aggaagcaga acaggccctt tccaaggcag aaaaagcgaa gtagcccttt
    39901 acaactcctg tatttgattt ttaatcctat ttataaaggc tctatagttt gtttcgtcta
    39961 taaacacacc agtggaggga cccgtaggag ggtccctttt ttttatgcct gaaatccggg
    40021 aataagccca tacgattagc ttatccagtt atttttttac tactttcacc cccataacat
    40081 ttcgattttt cttctttaat accatatttt atgagtaaat caggattacg ttcattccca
    40141 cgcaatttct ggacagtaat caccatggag ttttttgagc ggggctccta ctacagcgtg
    40201 atgtcaatac tttccgttta ccttgttctt ggggttaacg agggagggct tggtttcacc
    40261 aaggaaagcg ttggagtcat taaaagcgtt attacccccc tgctctacat cctgccaata
    40321 atctcgggcg ccattgccga taggtttgga tacaggaaaa tactcttttt ttcatttgca
    40381 gtgatgagtc ttggttacct gcttaccagc ttcagcaata cttatgctac cgttttcctt
    40441 agcttaattc taatggttac cggagccggg ttttttaagc ctatcatttc aggaaccata
    40501 gctcgatcaa ccgacagccg gaactcgtcg ttggggtttg gaatatacta ttgggccatc
    40561 aacctgggag cattcctttt tcccctgatt tttgtccctt tgcttaaatc aataggctgg
    40621 agctacattt tcataatggc tgccgtggga acgggatggt tgctctttct caacctcttc
    40681 gcttataagg agccaaccat accaaagagt aacaaaagcc tgataatggt attcaaggaa
    40741 atggtgctgg ttttaaagga ctacaagttt atccttatga tagtggttta ctcgggcttt
    40801 tgggttctct acttccagat gtttgactcg gttctttggt ttctaaaaga gtacgttgac
    40861 atgacgccag ttgatacttt cgtaaactca ttgcttgcca tttttgtaag caaccccaat
    40921 tggcatttcg atgcagaaca tgtaaccgtt gtaaatgccg gaaccataat tgccctgcag
    40981 ctggttattt cggccattgt aaaaaacacc cgggcattac ctaccatgat tgtggggatt
    41041 gccatgggaa caataggcat ggccgttctt gcaacgtcaa attatgcctg ggttttcatt
    41101 ggaggaatga tagtcttttc gctcggggag atgattgccc aacccaagtt tatctcctac
    41161 atcgggctaa ttgcacccga tgacaagaaa gcactctaca tgggctactc gttcctctat
    41221 ggagttatcg gcagtggaat tgggggcgtg ctaggcgctt attcgtatgt taagctagtg
    41281 aacgaaatgg gtcggccctc aatgttttgg ctgatgttta gctttatagg cgtggcaaca
    41341 attttgggct tgctggccta caacaggttt attctaaaaa agggaactgc caatccgcat
    41401 agctgataat tcaaaaacag aagctaatgc tttaaaaacg gtatcaaaac tgaaaaggtt
    41461 gtgcctaaat ccttttgggt tttgactagt atttctcccc caaagcccaa tacaatctcc
    41521 cgcgaaatgg ccaggccaag accagttccc ccggactttg tggtaaaatt aggtgtgaac
    41581 agcttgggta gcacctcttc gggaattccc accccattgt cggtaacatc aacccttatc
    41641 aacaaatcat tttcaggggc aagggttatg gtaacttttc catccttacc ccgctcaatg
    41701 gactgaatgg cgtttttaat gaggttgtta aaaaccctga taagctgctc cttgtcggcc
    41761 agcaccagcg gttcagcctc catgtagcta ataacgttga gcgcaacatt cctatatccc
    41821 ctgtaaaggt tggcagtgtc gtttattata tccagcagct ttaccggctc cctttttgca
    41881 gaaggaagct tggcaaatag ggaaaactcg cttgctatca ccgccagaga ttctatctgc
    41941 tccaaaagtg attgggcaaa acgctcgaat cgggcatccc agtcgggcaa gttatccttt
    42001 ttggccttca caagatgttg taggcttaac tttattggag ttaaggggtt tttcacctcg
    42061 tgagcaacct gcatggccat ctcgcgccat gcactctcac gctggttcct agccagctca
    42121 ttggcgctgc gggtaagctc gttaaccatg cggttgtact ctccaattag ctggccaacc
    42181 tcatcatcgc tactgtagtt aatggtttgg ttgttccctg taatgcttac tttgcctata
    42241 cgctctcgaa taagctgtag gggacgtgtt atctggctcg aaatgataat ggcaatggaa
    42301 attgctataa gagtaaggag agcgtaaatg ttgacaatcg ccaccacaat ggagtagagc
    42361 tcggagcgta gttcctcctg gcgtgtaaag tagggtaggt taaggtaggc gattgcctca
    42421 cgattcgaac ttagtatcgg aacgtaagcc gaaagaaact tagcctttcc tatattttcg
    42481 ttatcaagga atttcgggct gtggttggtt gccagctcat gccatgccgc agggttcatt
    42541 cttggtccaa ccagctgctt ttcaaatatt tcggaacgcg atgtagccag cagcattccg
    42601 gttgtgtcgt aaatattgat atcggtgtag aaaatgttcg atagctcaac caggcgatcg
    42661 cttagaaatc caatggccga gggatcgttg ctttgaatga ggtcaacatc tctatccagc
    42721 tcaaacatga tggaaataag cttttcgcta agattctttt cgttgcgctg ttcaaagttc
    42781 ttgatattgt aagcaatggt tgccgcaccc accatcaaga gcgacaaggt aacaataagg
    42841 ataattgccc gggtaatccg gttcttaaag ctggcatgag ttggtattag ccgcagggga
    42901 atgtcggcta ttttaaaaca tagtaaaaac ataacaaagt ataaaaaaag gcagtaggca
    42961 aaagaagcgg ttacgtttag gagtttctcc ctgggacggc taatgacaac tatatccgaa
    43021 ctaccaggac gataggtgag atggttgtag ccgaggaagt tctcacaggt aaaaacttga
    43081 gttgtggggt tgataccgct tactttttgc cggaagggaa attcacccga ttgggttata
    43141 agatttccgt tatggtactt ggcccaggag tactccttaa gctgctgctg ggaggctctg
    43201 ccctcaataa gcaactctgg atagcccagc atctcccatc ccggtctgct ggtaagttca
    43261 atgtatagat tacgaatgtt gtcacctatt cggtagttaa tttccccaac atagcttata
    43321 aacccgcttt gcgtgttcag gtagtaaaat cgtgatgacg gtagcataat gccgtaatca
    43381 tcaatcagca tcccaaaaaa cctgtcgcaa agcaaggtgg attttccatc aacatttata
    43441 attgatgatg gcgaacaaac agttgcccgc aggtcatacc ggttcaggta gttcttaaag
    43501 tagtccttac gtaggtagct gtagagctcg gcatttttct gctcaatatt cctaatgcaa
    43561 tcccggatgt aagaatcggt aagcattttt tggtagaggt tgggtaaaag catttcggct
    43621 agcggatcac gctccgagct aaggtttatg gcaagaactt tacgcacatt cagctctttt
    43681 tccgatgttt tgataaatac ggttgatacg gaataagctg aaaatatggt aacaatcacc
    43741 aaaatgtgtc ttagtaagag atgggttttt gagctgcggt aaacgaggag aacgtatgcg
    43801 accgtcaacc acatccaaac caaccccaaa ccggatggtt tgctggttag tgccagcgat
    43861 aggagcgtta ccactaagag tcctgatacc agtaggatta gcctttgctt taagctgagc
    43921 tgttccgcaa ctacgctaac ccaggtaatg gtaagtatga ccgcagaggc agcccaaagg
    43981 ctaacagata ggtatccaat tattgagtaa accgaaaggc taaatatacg gtagggttca
    44041 agggttaagg ttgaatgggt aaccagcgat gtaagcagct tgtccgttat ggggaataga
    44101 gctaaaaaaa gaatcagcac agaagctgca agcaggtata ggtgcatgtt cttgatatgc
    44161 ctcaatgaca atcgaatact ttgtcttatg aagagaacaa gcataaagac tagaatagca
    44221 tttataaaaa aatcgccaag agatgggttg atgaatgaat gggcgtagat ttcggggtta
    44281 aatattttca ttcccgacct ggagggaatt tccatgtaaa gggacaggta tcggattata
    44341 aaaataccgg ccgttgcccc ggctatcttc aacaatggct tatacctaaa aaacttttgt
    44401 ccgaagatga gcagtactgc gcaaactgcc attgcaaaag ctatccactg gagaatgggc
    44461 cataaacgat tcacatggcc attttggtaa atgtccagag ggacaaggta aaagggtgca
    44521 acgttatggg gtcttacggg tattgcgttg gaaaccaggt caatggaaat tctataatta
    44581 ttaaattcct taaacaacgg gctaaaggag tttttcaagt acttgttttg gtagggatat
    44641 tcgctataaa ttgggtttaa cacaagcagc tgcagcccct tacctgctgt aaaccattgc
    44701 gtgaagaagt actcgctttg aatttttacc agacctacct gtgaggaatc aaacggaacg
    44761 ccagagacat ccagcttgca cgaccaaaag cagagcgtat cgtgagaaaa aaggaatagc
    44821 tctccatccc acttaccctc aagcttcctc ttaaattcaa ctcccgattc gaatatttgt
    44881 ctggagctgg cgttgctatt aaccttaccc agaaactctc gggcaagctt gccggaatag
    44941 gttgtttccc tttcaactat tctctccaaa tgcttaacgt tgggcctata caaccttaca
    45001 tcaagcgcag acttcgacag gtattgggag accagcaaaa gtgcaagcga agcaatgaca
    45061 aggacgacaa tggcaaccag cctcttgttc atttcgatga gagttcggga tggattaggt
    45121 atgtagctaa attcatatca aaaatcaggc ctaaatcagg cgtgatggta aggttcacca
    45181 ttaataatgg taaaagcacg atagagctgc tccacgaata ccaaccgaac aagctggtgc
    45241 gaaaatgtca ttcttgatag cgataacctt tcggcagcag ccagcttcac cccatctgaa
    45301 aaaccgtaag ggccgccaac cacaaaagca atatcacgca aacccgaatt ttgcttcttt
    45361 tgaataaaac cggcaaactc ttcggatgat aactctctac ccttctcatc aagcagaata
    45421 acctcctggt agttccccat taccggcata agcacttctg cttccttttg tagctgaact
    45481 tccaccggca ttgatttggt attacgtagc tcgggcaaaa ctttaacttc gaagcggcag
    45541 tacctttcta tccgctttag gtaatcgttc attgcttcgg aaagatacgg tacgctggtt
    45601 ttccctataa gaatcaagtg aattttcaca aataaaatgg ttgaagtgat ttatccagct
    45661 tttaaatacg taatgcttaa catggtgaaa tttataaaaa catcaacctt aatcaagtac
    45721 tatcaaacct tctaaaaaat gggaacatcc tgccggtact ttaacccagc aaaaacacct
    45781 atttcaaatt ttactactaa cgcaaaggta aaaaatactt acacccaaag ctgcaccatg
    45841 gagtaaacaa aaaattcaaa aaaataattt acggcttgaa gagtgtcgga cattgactca
    45901 taaaagcttc cgtactgatg gagataattg gaaacaaaca tgttctacaa cgacacttgg
    45961 ttcaaaaaat gattataggt atatttggcc ttaggtataa agaataaatt aatctatagg
    46021 ttattggctc aatttttgag gatttttgta gtttagccgt gtggtaacaa taagaaaact
    46081 gtagaaatgg ggaaacaaaa aattaagctg ctttctctgt tcctgttgat tatgctgggt
    46141 tcgagtgcct ctgtgcttca acaggactcc aacgaaatta ttagtaaatc agcagaagcc
    46201 ttgcaaaatg gcgatgcggt taatctggca aaatatttct acaagaccat agaaattgac
    46261 atgctgggtg aggaaaactt ttacagtcag gcgcaatccg ttcagctgct caagaatttc
    46321 ttcgagaaaa acaaacccgt taagtttgcc gttagccatc aaggagcaaa ggaaacctcg
    46381 gctttcgcca ttgggcagct gcagaccaaa ggcaatacat ttcgcatctc catcttctta
    46441 aaatccgatg gtagccaatt tttcattcat cagctccgca ttgagcagga cgaggaagga
    46501 aacggaatat aaatggctac aaaccctgaa cttctcgttg aagcccttct caacctggca
    46561 attgctgagg atataggtga tggcgatcac tccacgcttt cgtgtattcc cccgtcggcc
    46621 accggcaaag tacagctaat gatgaaacag gagggcatta ttgccggact atcggttgct
    46681 ctgaaaattt tcaagaagat tgacagctcg gttgaagtag aattttttag aggcgatggt
    46741 gacttgctca gatatggaga tatcgccttt aaggcggaag gaaaagttca ctccctgtta
    46801 caggccgaac gcttggttct aaacgtgctt cagcgaatga gcggtgttgc tacccaaaca
    46861 cgggagtacg ttaaccgctg cgagggaaca aaagcaaaaa tcctcgacac acgcaaaaca
    46921 acaccgggca tgaggatttt ggataaaatg gcagtagtaa ttggtggagg gatgaaccac
    46981 cgcatgggcc ttttcgatat gatcatgcta aaggataacc atattgattt tgctggggga
    47041 atagaaaatg ccgttgcgtt ggcccataaa tacctgaatg aaactggccg aaagctggca
    47101 gttgaggttg aagcgcgctc cctcaaggat gtacaggtca ttttacgggt aggcggggta
    47161 aaccggatta tgctcgataa cttcagcgtt gaggccacac gcgaagcagt agggatgata
    47221 ggtggcaggg tggaaaccga atcgtccgga ggaattacgc tggaaaatat cagagactac
    47281 gccctttgcg gcgttgactt tatatctgtt ggagcactta cccaccagat aaggagtatt
    47341 gatatgaacc tcaaaaaaat ctgaatcgct gcttccgctt tacgtgtaat agctgagcaa
    47401 tctttttcgc aaccctccaa ccccacacgt tttctactta agcatcagct aaaggaaaag
    47461 cttgaacttg aacatttttc gcttttaact cgtattttta caaggaaaat ttctactgcc
    47521 taacttctat agatatgaat acaaaacggc tactgctgat ttcagccata ttatttacaa
    47581 ccattctggc gtggatatta cctgttgaag cccaaaatcg tcccccgcaa aatgtgtaca
    47641 gggttaccgt taccagccgt tacgagatta aagatggtaa gcgcaccacc aacagcaggg
    47701 ctatacggca agaaataaag gactcgcttg gaagaaccca tacaattctt aatcgcgatt
    47761 acgaaagtca gcgcgttacc agccatacat ggcatacatt ttccggaaaa ataattaccc
    47821 gtaccgacga attcgagaac gaacggttaa tcaggtttca cctttttacc tacactcccg
    47881 actcgcttat tgccagtgag gctatttaca gggttcaacc cggcgatacc tcgctttacc
    47941 tcaacatcag gtatggctac accaatagga ggcctgttca ggccattgcc acatctgcta
    48001 ccggtaaaaa agcctacacc ctaaaatcgg aatgggacag tcaaggaacc gagatcaagc
    48061 gaacggtaaa atcgaagaaa ggttttgccc cactggattc aatcgccctt ctcacctgcc
    48121 acccaaccta tgattccctg ggaagaaaag tggccgaaac aatatccaga aaatttctgg
    48181 atgggagttc taaaacccag caattccgct acgcttatga ctccaaaggt aataggatta
    48241 tgattgagga gcttaaccct tcaggtagca cgattgcccg caccgaaatg cagtataacg
    48301 acaagggtat gctaaagttc atatcaagat tcgatgcaca aaacaacctt attgactacc
    48361 aggccattcg ctacgagctt tacccctcac gcaaccggtt aaacatgatt attgagtact
    48421 gattgccaaa agggttgaac cgaatcagct taatgatttg ccctgcactt atagcaagca
    48481 gccgatcagc ataatcagct acaaaaacac acccttcccg ccagcaagca tagttgttgg
    48541 taaaacagaa cggataatcg ggctaacggc tggcttgctc ccctgatatt ggaagagtaa
    48601 agaagaactg gctacccttt cccggagtac tttcggccca aattttacca ttattcattt
    48661 caacaaactc ccggcatagt atcaggccaa gacctgtccc cttttcgttg cgggttcccg
    48721 gggttgagct aacctggctg ccaaagagtt tttttaggct atgggcatca attccaaccc
    48781 cagtgtcctc aacgcatacc tttacatggg aattttcaac ggagctccat atctgaattt
    48841 tatctccggc attgcagaac ttgatggcgt ttgatataag gtttcggata atcaggtcga
    48901 gcatatcgcg atcggcataa accgatgttg attcatccac gtggttggta atgacaatat
    48961 tcttctgaag ggcaacctcg tttagctgct gaagcctggg agtaaccacc tcgttcagga
    49021 agaatctttc cggtttcacc ttggttccct gcatttggtt tttggcccag gtaagtaggt
    49081 tttcgagcag ggcggttgtg tggcttacgt tggacgacag ctcgctgatt atttccttaa
    49141 aactttcctc ggtgaaatgt ccctccttgg caatgttaag catggtgata agcgaaaaca
    49201 atggacttcg gagatcgtgc gcaattatgg aaaatagcct atcctttaaa gcattcaact
    49261 ccctaagctc atccgcctgc cggctgattt cctccttctg ggcgaaaatt tcagcatttt
    49321 tctcgcttaa aagcttgttg gctttttgct tctggttatt agcccaaaac aaaatggcgg
    49381 caaacataag gagaataata atcaacccga tgtaaatcct ttggagtaga tgttggttcc
    49441 taatttcggc ttcgcggagc ttcatctgag tatttagcag gtctatctgc atacgctgtt
    49501 cggtggaaat tcgctcggtc tccttgagta tgttggtggt ttgctgaagc tcatggctgg
    49561 tttccattag cttacgagca gtagattcct tctcgtatga aatctgatta ataattcttc
    49621 cagcgctgtc aaccttggcc tgagcgagca tttgtcttgc cttgaactcg tcatcctgca
    49681 atttcttatt tagcatcatg tagagcctga agtactcatc agccttatcc ttgctgccaa
    49741 gtttttcgta aagcttatta tagttgaagt aaatattttt taaggctctg gcattatcaa
    49801 cttcttgcgc caacctttgg gcttcatcga gactcttctg tgcaccagta aaatcggaca
    49861 tttcaatctg ctctgttgaa atgttaacca gggcattggc aatatcgtcc tttcggccta
    49921 agcttctggc agctgcaagt gctgccaaaa agttgttata ggcctcgttg tggttgccct
    49981 tctcgcagta tattagccca aggtttgtat agattgtttt agcgccgttg acgttcccca
    50041 cctgcttgat gtttgccaac gccctatcaa aattagcaat tgccctgttc aggtctcttg
    50101 cctgccatgc ctggttggct gccttatcaa agtagtaggc tgcctggttg gcatcgccat
    50161 tcttctcaaa ttcagcggca agctgttcgt aattagctat ttgctggctt aaatcctgtg
    50221 aaaaaacgcc agaaggataa accaaactaa cgagtaaaac tgctactgct gctgctagaa
    50281 aacccctata ccctgctttc atgttgcgta aaagttgatt ttttataccc tccgaaaaaa
    50341 caatcgcctt atagacgtgg ccaacacgtt accttgcacg gtgctccttg agttaaatgg
    50401 aggcaaaaaa cactccaaaa actttgcgcc aaggtgttct gtataaatct attcaggcaa
    50461 ctcctattaa atcaataaca cgaaatgttt aacgttctag attttgatat tttctacggt
    50521 tttcaattta ttccgataca aaatgaacgg ctttttactt aaatcgtaag ttttctggga
    50581 taaaatgtaa aatatgaaat gccaacggag cagctgggtc aaaatcaata ctcgttttaa
    50641 tcataaaaac ttccggttgg aaatcggggg ttatgctaaa aatactgatt taggagtaga
    50701 tgaagatttt taggccatta gcctgagcat tttgggaact cccgttgagg caggttcacg
    50761 aataacggtg atatcgtctc tgaaggtggc tacctccttt ggatcaacca aaaagatggg
    50821 gcattggcgg ggaacatagc tcaagagccc tgcggccggg taaacgttca acgatgaccc
    50881 cacaacggca aagagctctg ccgtttgggt aagcttcaca gcttcctcca tggccggcac
    50941 cgcctcgccg aaccaaacca catgtggacg cagctgtgac cccagctcac acatatcacc
    51001 ccagtgtata tcgctgtacc cgatgtcata tacaagatca ggattaacag ttgagcgagc
    51061 ctttttcagc tcaccgtgca ggtgaagcac cttagtgctc cctgcccgct catgcagatc
    51121 gtcgatgttt tgagtgataa tgtaaaccaa aaagtccgac tcagccttgg caagagacaa
    51181 gtgggcagga tttggcatag ccttttgcag ctgcctgcgc cgttcgttgt aaaaattgag
    51241 caccagctgt ggattcctgg accatccttc aatgctggca acctccataa catcatactc
    51301 ctcccagagc ccatcggaat cacgaaacgt ttttataccg ctttcggcgc taacgcctgc
    51361 ccctgtcaac actaccaacc tttttttcat acccattcaa catgaaatgc ttaactacat
    51421 taaaggaatt ttatttttta accagcataa aattagtcat aaagctacac acattgtagt
    51481 ctaaaataca acgttccaga taatttaacc ttcagcacag cctaaaggta actttaataa
    51541 agggcgaaaa aacttagcgc tgttatgcca gccttttgaa tttcagcgct ccctcccggt
    51601 tatccacaat ttaggtaaaa gtactggtta caaatgcagg ctgaacaaat ggtgtttagg
    51661 tttgtagaac tttaacgcca atacttcttt ccagcaaaat cagaaaaaaa tagagctaat
    51721 gcctccgaaa atacagaatt attaaattta ccaaaatatt gacagaagaa aaatgcgcaa
    51781 actagctctg ttaattcttt tttgcatact atcagaactc ggcttatacc tccaggcaca
    51841 gccgctggtt gaaatttacc taaactccag ggggtctgaa tcaagaggcc ctgcatttaa
    51901 aagcgtggaa gaactccacg aagtcctggt acttgaccaa gccaaaactc catcgttcag
    51961 ggtggtgaaa cagtttaatg ctcagggtaa ggtagtatca gaaattaagt ataacagcgc
    52021 cgggggagtt cagaatgaag tcaggtggga gtataacata aacggctacc ccattagaaa
    52081 gactacgcat caattcatca actacaaggg ttgggtgaac gaggaaatca ccctgaaata
    52141 taacgacacc accggatacc tgaacgagat taacaaaagt gttgaaagta agattataaa
    52201 cacggctctg gtaagctgcg acagcaccgg acggattgtg gaggttaggg tattcaatga
    52261 aaagggcgag ctgagcggta tagaccgcct catctatgtg cctgccaaca accttatcag
    52321 ggtgctctcc tacaaggcca acgaccagtt tgtaatggca accgcatacc ccctcgatcc
    52381 tgacaaaccc gagcctaaat caaccctgaa aagagaattt aactcccatg gcgacgtcat
    52441 tctcgagctg ttgcctggat caaaaattgg acaaggctac tactacgagt atagctatga
    52501 tacttttggg aactggactg aaaagctaac ctaccagtgt acggttaccc ccaacgggaa
    52561 tgttaaagac aaaaagttgg agcatagaat tacccggaaa atagtgtact aaactttccc
    52621 cattttttag tgttttttta gagtataatt tttttgaggt tacggaatta gacgcactcc
    52681 atccattctt atatcgggaa taaccatgaa aatattaatt ctatgcaccg gcaacagctg
    52741 ccgtagccaa atggcagagg gttttctcaa gttgtttgga ggtaattttg aagtgcattc
    52801 ggctggaaca caacccgcac cagccgtaaa ttcatatgcc ataaaggtaa tgggggaggt
    52861 gggaattgac atcagccata accgaaccca tcatgtagat gagtttctta acgaggaatg
    52921 ggactacgtg attaccgttt gtggaggagc cagagaatcc tgccccgcat tcattgggaa
    52981 ggtcaaaaat cggcttcata ttggttttga agaccctgcc gaagcacagg gcagcgatga
    53041 ggaggtgctc acggtattcc gaagggtgag agatcaaatt ggggaagaat tccggaaatt
    53101 tcacgataac ctgttaaagc cttaaagcca tgccttcgaa aagccgacta aagttctttg
    53161 accgctacct gacgttgtgg attttcctgg ccatgctggt tggtgtgctg tcaggctacc
    53221 ttttcccatc catagcagta ttttgggatt cactaaaagc atcgcccgaa agtactacca
    53281 atatcccaat agccattggg cttatactaa tgatgtatcc acccttggcc aaggttaagt
    53341 atgaggagct gggcgatgtt ttccgcaaca ccagggtttt atccctatcg ttaattcaaa
    53401 actgggttat tgggcccatc ctgatgttcc tgctggccat tgtattcctt cgattcaaca
    53461 ttgactatat gtatggcctc atcctcatag ggctcgcaag atgcattgcc atggtgattg
    53521 tatggaacga gctagccgga ggcgacactg agtatgctgc cgggcttgta gccttcaact
    53581 cggttttcca ggtgctgttc ttctcggttt acgcatacct gtttattgcc atactgccag
    53641 gctggttcgg gcttccggtg aacgaaacca tttcacaaat taccatcgga caaatagcca
    53701 ctagcgtgtt catttacctg ggaataccct tcatcgccgg gtttttaaca cgactcgttc
    53761 tcacccgcat taaaagtaaa gcatggtaca acacccactt tattccgaaa attagccccc
    53821 taacacccat tgccctgctg ttcaccatcc tggtgatgtt ctcgctcaag ggtgaataca
    53881 tcgttaaaat tcctttagat gtggtaagaa ttgccatccc gctgatgata tacttcgttg
    53941 taatgtttct gattaccttc ttcatgagca ggcgaattgg tgccagctac cgtcaaacgg
    54001 caaccctgtc gttcactgct gccagcaaca actttgaact tgcaattgcg gtggctattg
    54061 ctgtgtttgg aatcaactcg ggcgaggcgt ttgccgcggt tattggcccg ctggttgagg
    54121 tacccgtgtt gatttcactg gttaacgtgt ctctaaagct taagaaaaag tattttagcc
    54181 aacaggctgt ttgatgaata tcaggtatgg ctttatagga aggttgtgta tcaaaattta
    54241 tcatttctaa atgccatgga gagctaccaa cccaaaacgg ctcacttttg atatagcagc
    54301 tgcaaacctt ttccatctaa agactgtaac gggttgctgg taaacaaatt aatctccatc
    54361 gtcaaaatat accttgggtt gacggatgtt atgttaaaaa aagttgaaat cggagttgaa
    54421 aaatgctaaa tttcaacttt caaacttttg gttatggctt caagaatagt gctgctggta
    54481 accaacaaag ttttaccttc tttccaattt gagcctcggc ttgatgggat gggttactcc
    54541 cttattaccc ttgatagaat tggctcggat gtggagctac cggcattcga cttggccata
    54601 gtaggagtag ctgatcgtgc aagctacaac gccatcctta aagtttttag gaaatacccg
    54661 gaaaaaccac tgctgttcta ctccggcaaa aatactgccg atgctcaaaa atctattccc
    54721 gagctagact caaccaacca cgtaaatcct gattttacca accatgagct gagtttgcaa
    54781 gtagaactgg ccatcacaag gaagtggttc gatcattcct ccatcttctc cgatggcaat
    54841 gcaggatcca atcatgttag gcttaaaaaa atcctttctg aggaacaagc ctggcgcgta
    54901 ctttttgaac agtcgccaaa cggagttctg cttagcgaca gcgccgggag tataattttt
    54961 acgaataatg caacaagcga aattctgggg tattcgtcct cccaattact taaaatgagg
    55021 tttcacgacc ttgttccgcc agaaacgctt cctcaagttg atcagaatat aaaaaaaata
    55081 cttgctggtg agcagcttat caccgaggtt tacaacctac gcaaggatgg cagcaagcga
    55141 tttgttcagc tgcacgaatc ccgagtttta tttcccagcg gcgaattcgg cattatggtt
    55201 gtgtccatcg acatgacaag agcaaaaaag gcagaagatg cgctggggga gtcgctggag
    55261 aagtacagaa ttctggtaga acaatccaac gatggcataa tttactcaaa ggatggcata
    55321 ataacctacg gaaacccacg cattgcagaa ctgctggagg ttcaacattg cgagctagtt
    55381 ggcaagccat tttataccta cctacctaaa agcgaatggg aaaaattaca aaaccgttat
    55441 aaaaatcggt tggaggggat aaaagaacct tcaatctatg aaaccttact tgtgagtgct
    55501 catggtagca atattcctgt tgagctaaac gtaaacctgg ccactattgg caacgaaagg
    55561 gttaccatgg tggtcgttag ggatatttcg ctacggaaag aggccgaaaa ggccataaga
    55621 gaaagcgagg agagctacag ggggcttttc aacaactccg gtgaggccat ttacatcatt
    55681 gacgccagcg gcatactgct tgatgtaaac ccaagcgggc ttaagctgtt cggtaaagaa
    55741 aaggaggaaa ccctaggctt aaccctaatg gagctggccg atcaggaaaa gagcagcacg
    55801 gaagaggtga agcggaagat taggatagcg caccagggtc aaactcaata cttagaattt
    55861 tggggaaaga aaagcaatgg cgatcgagtg ccaatggata tagtgcttag caagggcacc
    55921 tactttgggc ttcaggttgt gctggcaatt ggacacgata taaccgagcg aaaaaatgct
    55981 gaggctatcc tgcgtgaaag tgaggataag ttccgaaccc taaccgagca aatacctgtt
    56041 ggcatttacc ggttgacccc tgaagggaaa atactgtacg gaaatcccgc ctttgccaaa
    56101 acgcttggtt ttaacgatac cagcgaagtg ataggccaca gcatggatga gtttatagct
    56161 actaattacg acgattgggt tgaagaatca gtaaagctat caccgggtgc acctgccgaa
    56221 caatcgttcc gcagacaaga tggcataaat ttatgggcta acaatcaact ccgaagggtc
    56281 aaaaatgctt ccggcgaggt tgactacctc gatggggtgc tggttgacat caccgagcat
    56341 aaattcacgc tggatgctct aaaggaaagc gaggagcgtt ttcgggccat ggttacagct
    56401 atacccgata ggctgttccg aatcaatgcc gacggtgacc ttatcgattt tgccccttcg
    56461 gaacaactgc ttgattttga acttaacgct ggaatgatag ggcattccct ttccgaattt
    56521 ctaccccagg acgttttcca aaaatttact gatgccatta gcctggcaaa gcaaagcggc
    56581 aagcttcaga cttttgagta taagctttcc aaaaacaatc aggattactt caacgaagtt
    56641 aggctacttc aatcgggacg aagccttttc ctggtcctac agagggacat tacccagaaa
    56701 aaacagtttg aagctcaagc aaacatgctg gcgcaaacca tcatgaccgc caacgacagc
    56761 atctccatta ccaatacaga tggacatttt attttcgtta accccgcctt caccaaaacc
    56821 tatggctaca cccaatccga aattataggc aagcacacct ccatcctcaa aacccccgac
    56881 agggacttaa cacttgataa gacaattcat gaggaaaccc ttaagggcgg atggcaagga
    56941 gagctcatca acgttcgaaa agacggcgca gttttcccaa tattcctttc cacctcggcc
    57001 gtttacgatg agaacggaat cgcaatagcc catgtgggaa ttgctaacga cattaccgag
    57061 cgaaaaaaga ttgagcagga gctcatccga gcaaaggaaa aggcagagga atcggacagg
    57121 ctcaaaacag cttttctctc caacatgagc catgagatta ggtcgccaat gaacgctgtg
    57181 ctggggttca cccagctaat caaaagtgat gagcagctat cggaaaccgg aatgcagtac
    57241 atcgagctga ttcagaacag cggcaagcag ctcctgtcgc tgctggagga tattattgat
    57301 atttctaaaa tccagtcgaa ccagctacgg attgataaaa acgcattcga cctgaacgag
    57361 ctgatggatg agctttacct gatattttcc aaccagctta agcttaagga tgagtgcaaa
    57421 accctgctct tcaagcccga aaaaacctgt gtatctcctt ttgttatata ctccgacccg
    57481 gtgagaatta agcaaattct ctccaacctg ctgagcaacg ctattaagtt taccccaagc
    57541 ggaagtataa actttgggta ctccatattt tcggatgatg acgcttcaaa aattcagttt
    57601 tacgtgaggg acacaggaat aggcatctct atagaaaatc agcttctcat ctttgagcgt
    57661 tttaggcagg ccgacaactc atacacccga ttatatgggg gttcgggtct aggcctggca
    57721 atttcgagag gccttgttga actcctgggc ggggatatct gggtggagag taaacctggt
    57781 gaaggttcgg agtttttctt taccattccc cttgaatttt ccgaaaaaga tacgccagcc
    57841 cccgtccagc cttcaagcga agtgaaacaa cctgtaagcc agctaaaagg aaaaactgta
    57901 ctcgttgttg aggatgttaa cgaaatcagg ctttactttg aacgggtgct ggaacgaacg
    57961 ggtgcaaaaa ttctatttgc caaaaatgcc aaggaggcac gcaagctatt caagtcaaac
    58021 aaccgcatcc ggcttgttct gctagacatt ggcctttccg atgccgacgg ttttgaactt
    58081 gctgttgagt tcaagcgaat gcggcccaac acgcctataa ttgcccaaac ggcctacgcc
    58141 ctgcagtcgg agctaaacag gagcattgaa agcggttgcg acgactacat caccaaacct
    58201 ttagattccg ctgtgcttat tggtaaaatg cttagattga ttggtagcaa atcccagcag
    58261 tccgattagg ggactaagcg ttcaaccata gcaagggaat ggatggaaaa atagccccat
    58321 ctggtagggc ttcagggatg gaaacacagc caaaacctaa cttgtccggt aatccaactt
    58381 aacagctgaa cctaacatat tttgtaggcc aattatggcg tgatgtctta agtttcatta
    58441 aggctaatgg gaaatgaatc ttttcgtaga aaaactttgg ctgaagaatg gatacggcaa
    58501 agaatctaat agcgtcaatt ttagggcaca ttttaccaga gatagaaagt aaatcaagcc
    58561 aaatttttga agttatcctt ttttaaaaaa atcggaaact ttactaaaaa ccagcttaaa
    58621 atcctcatca accagttgtg aggtgccact atcagcatta aaagggtttt catgaatata
    58681 ctccctgtta aaatcaagtt cttctacttt aatattaatt gtgcggtagg ctccttttaa
    58741 tgtattaatt acctcaaaag agggaataac aagatctttt tttagggtga tagcataaat
    58801 ctgatcttcg tattttttca ataagccttc acggagatct ctcatcctct ggtagttgag
    58861 cattgaccga aacacctgac cctctaaatg ttcagtgata taatgatgga gcaggttgtc
    58921 tttttgcaga acggaatcaa aatgttcgat taaaaatgag tataacgcta tgttggcctc
    58981 actatcgagt ataaattttg aaactggtga tagccggtta aaagtggctc cgctgcaaaa
    59041 taaacaaacc tttgagttag caaaataatt gttgtagttg cttagcttaa gtatttgggc
    59101 aagaaaaccg cctatggagt aggcaaaaaa atcaaattca aaatttttgc tgatgtaggg
    59161 gtgctttcct tccttgcatt cttcaatcag ctgaattaca tcatagtaag tttgcaatcc
    59221 ggaccatata aatcgctgag gcattgaatg cagccggatg ctaatggcaa cattagacag
    59281 ggttgagtgc accacattcg ggtagcttct tttcctcatt tcgctcagct taaacatctc
    59341 ccttttattg ctccagcctt ttggcgcccg ctgcatatga aaagctatag gaaatagtat
    59401 aacgctgaat cctgtgcttt cagaaattgc cttggcccag ggcacatatt ttccccagtc
    59461 tttctcgttg aacccatgaa atagaaaaac cactctttgg gtggtggctt cgcctttagg
    59521 ttttatgatt tgatacttat aaaatctgtt ttcttcaata caaatatcct tggtgtcaac
    59581 attgctatta ggattgccaa cctcggtaga gtaagtatag ttgcactcaa tatctaggtt
    59641 atgcaatgtg caatgatatt tattcacacc tggaagaatc ttgtgtgatg acgactcaaa
    59701 gttgtactcc actatttcca gctcatcatg catatttcaa ctttttatta caactagcaa
    59761 aacactttac tatgacccta ggtgtatagt tttaaaaaaa tatttgttcc tctctgctaa
    59821 gggttggcaa aaaggtagta atccagggaa agtactcacc acacatcata gccggtaatg
    59881 aggatgtctc atttcaggtt cacactgcca gcggcttcgg gcattatcca acagtacgct
    59941 tacccgccca aatgcagata acctgcttca aaggtagctt atatcctaac actatctcaa
    60001 gccttcgtgg cagaattcac acttaaggtt gaagttttaa ggctttgcca ttttaagccc
    60061 tacaaaaaag taaccggggg cattggcgca tgaatgcaac ttgccaaagc gcacaaaatt
    60121 tagcataggt gggtgcaaaa gcaatatctc cgaaccgcca ggtctggctg ggctggataa
    60181 tagtaatgtt tacattgact ggtggaaaag ccaacggctc ggctagggag ccaagcgctc
    60241 aatcaaccac ttctcgttaa ccagggagta ctggatgcgg tcgtgtaggc gattctctct
    60301 tccctgccaa aactcaaaaa gagcgggttg aagaatgtag ccaccccaat ttggtggacg
    60361 cttaatggtt gttccttgaa actcctccat gaaatcagca tgaagctgct ccaggtacct
    60421 tctgttcgga attactgaac tttgcggcga tgcccatgca ccaatcttgc ttccctctgg
    60481 acgagtttga aagtagcggt cgctctgctg ctcgctcacc ctggacacct ttccctcaat
    60541 tcttacctgc ctttcaagct ccggccagaa aaacaccata gcagcgtagg ggttttgcag
    60601 caggttcctg gctttgcggc tctcgtagtt cgtaaaaaaa gcaattccat cgctatcgta
    60661 gtgtttcatt aaaacaacac gtgccgaagg cctaccctca aaggttgaag tagcaagggt
    60721 tatggcgttt ggatcgggta gcttggcatt cactgcctcc ttaaaccaaa tatcaaattg
    60781 ttcaaacgga tttggtagaa catccgtttt ccgcagcgat ttaagcatgt actcacgcct
    60841 aagctgtatg taatcaccca tggttcccga aaaaattata acatccttac tttcggttgc
    60901 tctgaaatag cacaaaagca tcaacccgct aacaaaagta gcaggtaatt gttcaaccct
    60961 caccggggtt attatttcag cttctactcc ctgcttttta tattgcagca tcgggaaaaa
    61021 aatcatgcct atcccctttg aaaataaaaa ctctacccta aacccttagg tcatggccta
    61081 aagtagcatt atctactcct ccagaatttt tttttggatc aaaaacagta gtttttattg
    61141 tgctcaatgc agcggatgac ttaaattttc gtcatttttg tataactaaa aaaaccagaa
    61201 tatggatgta gaaacaagaa ccacagccgg acaggttatg ggtgttatcg ggatagtgtt
    61261 aggagttgtt tcgctggtct tggcctttat accatgtatt ggcgttatgg ctttcattcc
    61321 gggagtgctg gcccttactt tcagcataat ctctattgtg caggcttcga atggttatgg
    61381 gtctaagggc ctgggtatag gtgccttgat tgtttcgctg gtatctattg tgcttgctgc
    61441 cgcatggctt attgtcttta gtggcacatt tgcctttgcc gacagggcaa ttaggaatag
    61501 cgacaagata gaaatttttg gcaaggaatt tggcaaggcc tttgaggaag agtttggcaa
    61561 gggagatgtg aacttaaaaa ttgtttccga cagcctcgaa aactcacttc gccagctcga
    61621 aaacgagctg gacagtctgg atggctacat accggaagag aaggctcgca aggcaggaca
    61681 agctgctgcc agagccttaa aaaaatctac acgggtggta attgaaaccg actcgggtaa
    61741 gattcaattt gaaaccagca ataaaagcca ttaaagtagt tgaaacctcc gtcttgcaaa
    61801 aagggtatta acgtttacgc ttctgttaat gcacttttta ttgtcgttat cctggcagtt
    61861 tttatgtact cctttgcgtt caagaccgaa aaatatccaa ttcctgcact gctcacccaa
    61921 agtacagggt taatacctcc gagccgggga ctttcaaaaa gtttttctga aattgtaagg
    61981 ggaaatatat ccgcagcaaa gcttatcaac cctcattctt tgcagatatt cgcatttttt
    62041 ttgattcagc tggtcatgcg ggttgccggg tttgcagcag ctaaaattat tggcagccca
    62101 tttcccgtaa aaggtatcgc ggtagatgtt tgcatatcgc tagcgctttt tgtcttctgc
    62161 tttgccccgc ttatttcata tactttcagg ctattcgttc agctctttta gaagctgctg
    62221 aaacgaaaaa ccctccgttg cccttattcc tttagcagta agctgaaggc ggctgcaatc
    62281 ggcaaagtaa tcgtgttcat acagtaaaat gtagttcccc cgaagcgcct cgttgagaaa
    62341 ctgctccttt tcctgaagcg ttttaagcgg ttcaacatcg tagctcataa tccagggtaa
    62401 aggaatttgg gcagtgaaag ggaaaagatc ggcacagaat accagcgttt tcccacgata
    62461 gctgattagc ggaataagtt gtccgcgggt gtggccgtta aaaattctaa gagaaagtcc
    62521 gggtatcaat tctccttcgg attctatcag gtgtagtgca cccaactcct tcataggaag
    62581 aatattttca ggaagaaagg aatcgtcctc ccgggagttt gggtgggtag cccactccca
    62641 ttgtgcggcc gaaacatggt aaagggcatt agggaatgtc agctgaaatt tcccatggga
    62701 atcacggaaa atccctccac cgcagtggtc aaaatgcagg tgggtgagaa tcacatcggt
    62761 aacatcttct ggcctgtagc cccttgctgc taaaccgggc aacaagccct cgccgccaaa
    62821 agcgtaaaca tggctcataa actttgcatc ctgcttactg cctatcccat tgtcaatcag
    62881 cacaaccctc ccgttagcct caacaaccag cgatttaagc gaaaggggta tcaggttatt
    62941 ctcatcagag gggtaaacct taccccacat agtttttgga actacgccaa acattgcccc
    63001 accgtcaacc ttaaagtttg atatgttaaa ctgcgaaatc ttcatacatg ttgagttaaa
    63061 atattttaca aagttacaat tcctgtaaca cgcaacacaa actcctcggg cacaacatag
    63121 cgcttaatgc catagctggc gaaatatagt tcattcccgt tcactctgaa ataaaaaatg
    63181 agctccccaa caagtaaatg ggcaaagcat actctacttg cagcatttat tgtcaagttg
    63241 attggttaaa actcaaccat tgaattaggg aatctgggaa aaattcaaag ggtaagtgca
    63301 tttctgtaca aaaactgttt gataaaaaat ctgcttaagg caaaatcaac aggcgccaat
    63361 aaatttttgg ctacacttat gagcataaag gtacttcgta ttaccgctat tcattaattt
    63421 cgagtaaatt gcaagtgata taaattttgg catatatgct tgtaaagact tttggaagtg
    63481 ctgttcatgg cataaaggcc accaccataa ccattgaggt gagtgtttcg caagggataa
    63541 acttctttct tgtagggctt ccggacaatg cggtgaagga aagccagcaa cgaatcacct
    63601 cggctttaga aacaaatgga tataggttcc caaagaaacg ggttgttatc aacatggctc
    63661 cggccgatat aaggaaggag ggctcggcct acgacctacc actggcattg gggattctgg
    63721 cagcatcgga acaggtaaac cctcaaatgc tgccggatta cgttgttatg ggcgaacttt
    63781 cgctcgatgg ctccgttcag cccgtaaggg gggtgcttcc cattgccatt caggctcgtg
    63841 aggagggctt caaagggttc atcgtgccca tacaaaatgc acgggaagca gccgtagtaa
    63901 acgatttgga tgtttacggg gtatcatcaa ttcgggaagt cattgatttt ttcaacggaa
    63961 acgaaagcct aaagcccaca attatcaata cccgtgagga attttttgcc aacgccaaca
    64021 gctacgatgt tgactttgcc gatgtaaagg ggcaggaaaa tgtgaagcgt gcgctggagg
    64081 ttgccgctgc cggtgggcac aacatcatca tgattgggcc tcctggcgcc ggtaaaacca
    64141 tgctggccaa acggattcca accatcattc cccctcttac gctgcgcgaa gcgctcgaaa
    64201 ccacgaaaat tcattctgtt gccggaaaga tagaccgcga cacctctcta atgacccgac
    64261 gtcctttccg tagccctcac cacaccatct cagatgtagc gctggttggt ggtggacagt
    64321 acccccaacc cggcgaaatt tcgctggctc ataatggcgt gctattcctc gacgagctgc
    64381 cagaatttaa acgaaccgtg ctggaggtca tgcgtcaacc gctcgaggat agggttatca
    64441 ccatatccag agcaaaattt tccgtggagt accccgccag cttcatgctg gttgcctcca
    64501 tgaacccctg tccatgcgga aactataaca acccttttaa agaatgcgtt tgctcaccag
    64561 gcatggtgca aaagtaccta aaccgaattt ccggccccct cctcgataga atagacatac
    64621 acatagaggt tattcccgta ccgttcgaga agctggccac aacaataaac tccgaaacca
    64681 gcgaaaccat ccgtaaaagg gtaattaaag cacgccaagt tcaggaagaa cgatttgtag
    64741 agtggaagga agtttactgc aatgcccaga tgacctccaa gctgatcaga aaattctgtg
    64801 agctcgacag tgctggatcg tcgctgctaa aaactgccat ggaacggctc aacctctcag
    64861 caagggccta cgatagaata ctaaaagttt cccgtacgat tgccgatttg gatgattcac
    64921 ccagtatcaa acccgagcat ctggccgagg ccatacagta ccgaagcctc gacagagaaa
    64981 actgggcagg gtaaaggctt ttatcttatg ctatttttta aactctccca attactaaaa
    65041 gttgaataaa aacacttcga gtcaacccga aaaaaaacca aatatgcttt cattaccatt
    65101 caacactttg tatattggcg agtttaaaaa taaaagggat ttgttgctaa accatacttg
    65161 catattgttg aatttctgat ttataaccta actataattc aacctaaaaa caactagtat
    65221 gataatttcc aatgtattct gggttgttcc gattgcatcg gtgcttgccc tgctatttgc
    65281 ctactacttt ttcaggcaga tgatgaagga ggatgagggt acggatatca tgaaaaaaat
    65341 tgcccttcat gtgcgaaagg gcgccatgtc gtacctaaaa cagcagtata aagttgttgg
    65401 gcttgttttt gtggtgcttg ttctcctctt cgcacttctg gcctatggtc ttcacgttca
    65461 aaatggatgg acgccattcg catttcttac cggcggcttc ttttcgggtt tggccggatt
    65521 tttgggtatg aaaaccgcca cttacgcatc ggcccgcaca gcaaatgctg cacaaaaatc
    65581 gcttaaccga ggtctgaaaa ttgcttttcg ctcgggtgcc gttatggggc tggtggtggt
    65641 tggactggcg gttctcgaca tttcgctatg gtatcttatc ctcaactact ttatagacga
    65701 gcccgaccca tcccagaagc taattgtcat cactactact atgcttacct ttggtatggg
    65761 agcatccacc caggcgctat ttgcccgtgt tggaggtgga atttacacca aggctgctga
    65821 tgttggcgcc gaccttgttg gtaaggttga agccggcatt cccgaggatg atccccgtaa
    65881 ccctgccacc attgccgata atgtaggcga caacgtgggc gatgtagccg gaatgggtgc
    65941 cgacctgtac gaatcctatg caggttcaat ccttgctact gctgcactag gggcatcagc
    66001 atttgcacta tttgaaggta acatgcagta caaagctatt ttagctccca tgcttattgc
    66061 gtcggttggt attgtgctat cgctgctggg cattttcatt gtccgcacca aggagaacgc
    66121 caccatgagg gatttgctcc gttcgcttgg caggggtgtt aacgtaagct cgctgctaat
    66181 tgccctctca acttttggag ttctttatgg acttgggctt caccactggc taggaatttc
    66241 attttcggtt gtatcgggaa ttgcagcagg tgtaatcatt gggcaatcca ccgagtactt
    66301 cacctcccat tcgtacaagc ccacccaaag ggtatccgaa agttcaaaaa ccggaccggc
    66361 taccgttgtc atttcgggca tcggcctggg aatggtatca acagccatac cggttatcac
    66421 cataaccctt gccattttga tggcattttt atgcgccatc aactttgacg ttaagcatat
    66481 gctggaagct caaaacatcc agctggggct ctatggaatt ggcattgccg ccgtggggat
    66541 gctgtcaact ttaggaataa ccctggcaac cgatgcctac gggccaatcg ccgataatgc
    66601 agggggaaat gccgagatga gtcaccttgg ccctgaagtc cgcaaacgta ccgatgccct
    66661 tgatgcattg ggaaacacta ccgctgccac tggtaaaggt tttgccattg gttcggccgc
    66721 cctaacagcg ctggccctgc tagcttcgta catcgaagaa attaagattg gccttgtgcg
    66781 aataggtgaa aacgttctca cctttgccaa tggtgaaacc gttgagactg ccaaggcaac
    66841 cattgttgat tttatgagct actaccagat aaacctgatg aatcctattg tactcgccgg
    66901 aattttcata ggagccatga tggcattcct cttctcgggg ttgaccatga atgcggttgg
    66961 tcgcgctgcc cagaagatgg tggaggaggt tcgccggcag ttcagggaaa ttaaagggat
    67021 aatgacaggg gaaaccgagc ccgattacga gcggtgtgtg gccatctcta cccggggagc
    67081 gcaaatcgaa atgatccttc cctcttcatt ggctattatt gtaccaatac ttaccggttt
    67141 gctgctgggt gtagctggcg tgatgggctt acttaccggc ggacttgggg caggatttgt
    67201 gctggccatt ttcatggcca actcgggagg ttcttgggat aatgcaaaaa aatacatcga
    67261 ggaggggaac ttcgggggta agggttccga ttgccataag gctgccgtgg ttggtgatac
    67321 cgtaggcgac ccattcaagg atacctcagg cccaagcctc aacattctca tcaagttgat
    67381 gagcatggtg tccat
//
LOCUS       DAOSHM010000007        64655 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_354_length_64655_cov_21.133653, whole genome shotgun sequence.
ACCESSION   DAOSHM010000007 DAOSHM010000000
VERSION     DAOSHM010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 64655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 64655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64655
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_354_length_64655_cov_21.133653"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..637)
                     /locus_tag="PK839_02065"
     CDS             complement(<1..637)
                     /locus_tag="PK839_02065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG71551.1"
                     /translation="MNKRIFILIIIDLMLILVAFYLSVLLKGVSFASYLESYQNALLL
                     FALIWIATSIVFEKYVFTTHSARKISANIIKSNLLAFAVVTFLIYFTRSDRYSRFIVF
                     NTILMVTLLELFIYNLWAGLKRIRVIPDNLLGMIERSVKRRTPPIQTTRAPNPMRVEL
                     IKRTLQAEFGNEVYEYLNTNTRLFDDKTLIVSTTTPFNIINQPDGYFDTLIN"
     gene            955..2067
                     /gene="mtnA"
                     /locus_tag="PK839_02070"
     CDS             955..2067
                     /gene="mtnA"
                     /locus_tag="PK839_02070"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF004326.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HOG71552.1"
                     /translation="MKVNGVNYRTIWLEDGSSPCVKIIDQRYLPHRFVVEPIESFDGL
                     LRAISEMHLRGAGLIGVAAAFGIYLAVWEQRNLVNFQEALEKCALKLIKTRPTAVNLR
                     WAVERQLEVLAGVENPDDRVAIARQTAIDIAEDDAGCCRKIGLEGVGLIEDIARNKNG
                     GVVNILTHCNAGWLAFTDYGSALAPVYEAWRRGVRLHVWVDETRPRNQGASLTAWELL
                     NEGITHTVIADNTGGHLMQHGLVDLVLTGADRITTNGDAANKIGTYLKALAAHDNGVP
                     FYIAAPSSTFDWSLSDGLSQIPIEQRHPDEVRYVGGWLDGRLQNVLVCPDESPAANFA
                     FDVTPARLISGIITERGICKPLPAGIKKVFPEKFNY"
     gene            2064..2669
                     /locus_tag="PK839_02075"
     CDS             2064..2669
                     /locus_tag="PK839_02075"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HOG71553.1"
                     /translation="MNEKGIIKFRCSWVNADPLPYEAVADLNRWRCILYNKGLVGVDC
                     NGVGFGNLSKRYLHNQFVITGSGTGQLTELTTMHYTLVTDFSIGRNWVEAKGPVIASS
                     ESLTHAALYQCDKAIKAVFHIHNLELWKRIIGKVPTTLSNAEYGTPQMAMEIKRLYRQ
                     TDLRKTRVLVMAGHQEGIMAFGLDADEAGKRILSLMKEYNP"
     gene            3075..4841
                     /gene="sppA"
                     /locus_tag="PK839_02080"
     CDS             3075..4841
                     /gene="sppA"
                     /locus_tag="PK839_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HOG71554.1"
                     /translation="MKSFFKYLLATIVGVLLSFLLIFLIMMGIAGSMVSSADKAVEVK
                     DNSILYMDLKNEIVDRASSNPFETLDLATFEPQKNLGLNEILKAIAKAKDDPRIKGIL
                     LEFDVVNAGAATTGEIREALREFKESGKFVVSYSDTYSQKAYYLASVADSIYLNPEGM
                     VEWLGLRSEIMFYKKAFEKLDVEPQIIRHGKFKSAVEPFMSERMSPENREQTLTYLSS
                     IWGSWVDEISKSRNIAVAELNRLADNMEVYDGKSCMKSKLVDSLIYKDQLLEKLKAFT
                     GIPENRNLNTVTLEKYFKVPPVQKTAFSKNKIAVIYAQGEIGMGDGSNTAIGSEGLSK
                     AIRQARRDSTIKAIVFRINSPGGSALASEVIWREVELAGQAKPLVVSMGDVAASGGYY
                     IASPATTILASPTTITGSIGVFGLFFNLQKTLDKKLGINVDVVKTNAHSDFFSMFRPM
                     TAEEKAVGQRFVEQTYQTFIGHVSQGRGIPVERVDEIGQGRVWSGANAKEIKLVDDFG
                     GLTAAIKLAAEKANLDTYRLVELPKQKSPFEELLKGFTTEAKAMLVKDDLGKGYKYYN
                     RVMQMVENQGVQARIPYDVEVY"
     gene            complement(4948..6141)
                     /locus_tag="PK839_02085"
     CDS             complement(4948..6141)
                     /locus_tag="PK839_02085"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HOG71555.1"
                     /translation="MMASLNDKLKELYKTTPVVAGDIYRHIHQNPELSFRESGTSSYV
                     ASFLESLGIGIIKLDKSYSLLATIDGTGEGKTIGLRAELDALPIDEKTGESYASRTAG
                     VMHACGHDFHLASVLGAAIILNNLREFWSGKVVLVFESGEEELPGGARAILESDEFQE
                     VKPDYMVGMHVLPELTTGKVGFCPGRYMASGDEVYLTARGRGGHAALPHTLVDPIVIA
                     STIILSLQTLVSRKAPSNIPSVLSFGRVTANGSTNVIPDEVSISGTFRTMNEEWRAKA
                     HDLIVQMASDIARSTGGECSVEIRKGYPSVYNDPILTQTAVEVASSIFGSENVAPLEP
                     RMTTDDFAYFSQAIPSVFFRIGVGTPGAPTLQLHRPDFRIDEQAFQTSLPMLTGLCLK
                     LLTSS"
     gene            6602..7699
                     /locus_tag="PK839_02090"
     CDS             6602..7699
                     /locus_tag="PK839_02090"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HOG71556.1"
                     /translation="MMKRILISGFACAMLLIGIVDAQSQVARIYFKDSNHDYGVIKED
                     DGVAYYEFQFSNTGSTPLIIQRVETSCGCTTPDWPKEPIAPGKNGRIKVGYNPSGRPG
                     AFSKTITVYSNAETPTVILQIKGEVIPHVKTPDEIFNISIGGKLYFEKTHIPFSRLFP
                     GKIYTDTLKFMNKSSEVVKVAANAGGLNFLTVRVEPQSIKPKQVGNIIVTYDPQKRND
                     WGFVMDRFMLSINDSLITNTPISITGSIEEDYSALSASELEKAPRVEFNTTSYDFGQK
                     AEGEPVEYSFTIKNTGKSDLQIRKVKASCGCTTVQPSKNILKPGESTEIKALFRTNGY
                     SGRQSKSITVITNDPKSSTTVLRLSGNVVKK"
     gene            8092..9435
                     /locus_tag="PK839_02095"
     CDS             8092..9435
                     /locus_tag="PK839_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic 2,3-diphosphoglycerate synthase"
                     /protein_id="HOG71557.1"
                     /translation="MNKRNVVIIGAAGRDFHNFNTFFRNKEEYNVVAFTAAQIPDIDG
                     RKYPAELAGKLYPNGIPIVSEEELPRLIKEKNVHDCVFSYSDVKYQNVMRISSIVNAA
                     GANFMLLGPNETMLKSTKPVIAVVATRTGCGKSQTSRKVIEYLMGLGLKVVAVRHPMP
                     YGDLVAQKVQRFATVEDLKKHKCTVEEMEEYEPHVVRGNVIYAGVDYEAILREAEKDP
                     SGCDVILWDGGNNDFSFYKPDLTITVADPHRPGNEVSYYPGEVNLRMADVVVINKMDS
                     AAPEGIQMVRDNIRRVNPKAIVVDGASPIKVDNPELITGKRCLIVEDGPTLTHGEMKI
                     GAGTIAARKFGASEEVDARPFLVGKLKETYQIYPNIGRILPAMGYGEQQLKDLEGTIN
                     ATDCDSVIIGTPIDLNRIIKIKKPSTRVYYDLVEIGLPDLKGVLEKFVKQHKLDK"
     gene            complement(9608..10963)
                     /locus_tag="PK839_02100"
     CDS             complement(9608..10963)
                     /locus_tag="PK839_02100"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOG71558.1"
                     /translation="MPFNRDKVEQWSLAYWLLKCHENFFFKLYFRTEIVGIEKLPDNA
                     TLIFAPNHQNTLMDALAILTLKRDFQPIFLARADIFKKPFLSKILTFLKIMPVYRSRD
                     GFENLLLNDRIFQKTLDVLQNRNGLVVLPEGSHEGVKKLRQLKKGIARIAFQAEDAAN
                     GKLGIQIVPVGLEYSNYVAFRSNLLIRLGEPIEVDRYLELYRQNRAQAYNALIAELEQ
                     RMRLEMINIEDENYTAYLTILELFSARYIKERKLPKSQNQKFQVDKKIISLLDRLKNT
                     NASEFEEIIKNANDYGRIISANGLSVKLLPASSKTLRDLPIRLLLLLITMPVALYGAV
                     NNAIPIAVPYLLSKKFKDVQFHSSIKHAGALLLTPLAYLVQTLVVGFVLKNIWLALAY
                     LATLPLGAAAFYLWRREFFRFTSQLSLRRFRKAKAEEFDLVNDLNNQIYRTVTGLVDK
                     E"
     gene            11191..11745
                     /locus_tag="PK839_02105"
     CDS             11191..11745
                     /locus_tag="PK839_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014774449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HOG71559.1"
                     /translation="MPVKIRLSRHGRKKNPYYHIVVADSRAPRDGRYIERIGSYNPLT
                     NPATIELNFDRALDWLQKGAQPTDTCRAILSYKGVLYKNHLLIGVKKGAITMEQAEAK
                     FDTWLKEKEGKIKAKIDLVENSNAKRKNERLEAEAKVREARAQAVAKKRAAEAEKEAK
                     KSSENSAQDAVEANADTEAGSSEE"
     gene            11752..12282
                     /gene="rimM"
                     /locus_tag="PK839_02110"
     CDS             11752..12282
                     /gene="rimM"
                     /locus_tag="PK839_02110"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HOG71560.1"
                     /translation="MTDTQLVEVGFIQRTHGVKGEIQISVASSAGVLPEELELVFLDI
                     EGIPVPFFIQGIVAISDEILIVKFDEVDSVEEAAELVGLRVLLPCGCLSNDDGGVPFS
                     SLVGYRLFSTKNEELGIITRYEEFAYNALFAVTAKSGKELLIPAAENLISRIDDAERI
                     VWMDIPQGLLEVNSMD"
     gene            complement(12256..12708)
                     /locus_tag="PK839_02115"
     CDS             complement(12256..12708)
                     /locus_tag="PK839_02115"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HOG71561.1"
                     /translation="MTIKPHSYFHSTPIQIRVNDIDPLNHVNNSVYQQYFDLGKIAYF
                     NRVLGEHLNLETEGLVLANITIDYLKPITLYDSIEVFTRIYEMGTKSLKMGQEIVNRV
                     SKEVYTTSKSVLVCFSNSSGLSIPIPLLWRERIADFEKEGLIHGVNLQ"
     gene            complement(12689..14590)
                     /locus_tag="PK839_02120"
     CDS             complement(12689..14590)
                     /locus_tag="PK839_02120"
                     /inference="COORDINATES: protein motif:HMM:NF014740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HOG71562.1"
                     /translation="MIPPVKHAPRWLVFVIDLCICLVSITLSYYVRFEFRIGSEQVNA
                     LKLVAPLVLFVRALAFISFHTYAGLIRYTSLKDAERIFMVTTSSTIVLFTINFIVYSK
                     SGQFLIPNSILLIDFCGTNILMIGSRLFYKTLYYTIKNVDIEAENIVIVGIEQFAASA
                     KRAIDKDNMAGLKVVAFIDPNCKQEGNSIEDVRIYNTSKIEFLVQKYGVSRILIAQKN
                     LDPVKKNKIIDKCLQLNVKVQVIPDATAWINGELNTSQLKSINIEDLLERPPIVLDKR
                     RIQNYIKNKVVLVTGAAGSIGSEMVRQISKFSPKKIVLFDQAETPLYDLELELKENLH
                     FYDFESVIGSVTNDYRVNKLFDVFKPNVVFHAAAYKHVPMMENNPTEAVFNNVLGTKL
                     IAETAVRYGVEKFVMISTDKAVNPTNVMGASKRIAEIFTQSLSCQVDTNFITTRFGNV
                     LGSNGSVIPRFRKQIEKGGPVTITHPEVTRFFMTIPEACQLVLEAGAMGNGGEIFIFD
                     MGEPVKILDLAKKMIKLSGLTLGKDIQINFTGLRPGEKLYEELLNNTENTIPTYHPKI
                     MIAKVPDLIPENVIPKINRLIETLPEHNNFKVVSLMKDIVPEFISRNSIYEDIDRQKK
                     YIVNDYKTA"
     gene            complement(14752..15183)
                     /locus_tag="PK839_02125"
     CDS             complement(14752..15183)
                     /locus_tag="PK839_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG71563.1"
                     /translation="MRAKIAKLLVIAASFFLPSSCGEDEPEIVPDVPVHIEFYLNEPF
                     YNNITVPMGAVKIPNEGYNNNGIIVFRLNQEEIVAFDATCPQHIAVNASVALDENGSG
                     TATCPHCHTVYYFYNNGYPTKGYKLKSYQVSFRGDLVGIYN"
     gene            15351..15998
                     /locus_tag="PK839_02130"
     CDS             15351..15998
                     /locus_tag="PK839_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG71564.1"
                     /translation="MDGGDILFIIIVTILGIISSVSRKKKEAAKRAMKQTEAEEPKNY
                     SEGQPVEIPENNNEDIYTWPQQSVSEFPSNDTMQDFTSNSNPRNEGKSVLSGWDNMEG
                     EVKSVEYEMPSVQVSEEELYAPKKYEELDKIPTSSGSSLLDQPVSPYETTYEGLASSY
                     QPIAFEEQEEESKAPDMIFEYEKVLEQESNQAGILDDFDPAKAVIYSEVINKKYF"
     gene            16108..16581
                     /gene="greA"
                     /locus_tag="PK839_02135"
     CDS             16108..16581
                     /gene="greA"
                     /locus_tag="PK839_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HOG71565.1"
                     /translation="MSKVNYLTEEGLEKLRKELDHLKSVERPAISKMIAEARDKGDLS
                     ENAEYDAAKEAQGMLELRISKLEDLVANARVIDESKIDTERVQILNRVKLKNLKTNAV
                     VEYMLVSESEANLRENKISIATPIAKALIGKKIGDIVEVQVPSGLMKFEILSISL"
     gene            16594..16989
                     /locus_tag="PK839_02140"
     CDS             16594..16989
                     /locus_tag="PK839_02140"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008863076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HOG71566.1"
                     /translation="MASIFSKIVNGEIPSYRVAEDEKYFAFLDINPLAEGHTLVIPKQ
                     EVDYLFNLDQETLSGLVLFAQRVAKAIESVIPCKRIGVAVLGLEVPHAHIHLIPINSE
                     SDIDFHKPKLKLNPEQLDAIAQKIATKFE"
     gene            complement(17054..18316)
                     /locus_tag="PK839_02145"
     CDS             complement(17054..18316)
                     /locus_tag="PK839_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HOG71567.1"
                     /translation="MLKIFTRFDSKNHRPVMGAMEIFKFIGPGLLVTVGFIDPGNWAS
                     NLAAGADYGYKLLWMVSLSTIMLIVLQHNVAHLGIATGLCLSEATTKYLKPGVSRFIL
                     GTGMLASVSTSLAEIMGGAIALQMLFGIPIKIGAVLVLAFVLVMLFTNSYRAIEKWII
                     AFVSIIGLSFIYELSLVNIDWPEAIKSSVTPSFPEGSMLVIMSVLGAVVMPHNLFLHS
                     EVIQSRQWNLQDDRVIRRQLDYEFFDTLFSMLVGWAINSSMILLAATTFHKTQTPVSE
                     LSQAQNMLIPLLGSSAADIFAVALLLAGISSTITSGMAAGSIFAGIFNEPYDVKDNHS
                     KLGILLSLVAAVLVIFAIDNPFKALLISQMVLSIQLPFTIFTQVRLTSSEKVMGKYKN
                     SPFTKWTLYGLGAVVTLLNLMLFVSFLG"
     gene            complement(18508..19002)
                     /locus_tag="PK839_02150"
     CDS             complement(18508..19002)
                     /locus_tag="PK839_02150"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HOG71568.1"
                     /translation="MDSREKVSIDKALTRLQNLCARQEKCVWDIMQKLKLWGINNEEA
                     QKIVSMLVDEGFVDDRRYAQMFVREKSQLNKWGSIKIAAALRVKKINESIIKEALLEL
                     QPQFDRATLENIITTKANQVKAKSPSDLRAKLVRFGLSRGFELEHVVKTVDMVMKSNM
                     TDEA"
     gene            complement(19025..19885)
                     /gene="prmC"
                     /locus_tag="PK839_02155"
     CDS             complement(19025..19885)
                     /gene="prmC"
                     /locus_tag="PK839_02155"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HOG71569.1"
                     /translation="MKSYTQRQLFDEVGLVLNQFFTSGEVNSLRRMLLEHVLKIPYYH
                     VHLTPNAQVSPSLSKNILDMTGQLVRGVPIQYLLGETTFMELNLTVNPSVLIPRPETE
                     ELVRWVIADCHLPKPKILDIGTGSGCIAIALAQNIPDSTLTAVDISPDALKVARHNAK
                     IYSASICFVEADILSGSLIPNAPFDIVVSNPPYVRSSERRFMHKNVLDHEPPLALFVS
                     DDNPLQFYKAIVSKSSKLIKQGGRLFLEINEEFGNQMVELLEANHFGKVTLRKDINER
                     YRMISGTYTG"
     gene            19929..21026
                     /gene="ribD"
                     /locus_tag="PK839_02160"
     CDS             19929..21026
                     /gene="ribD"
                     /locus_tag="PK839_02160"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HOG71570.1"
                     /translation="MDNLDVDKLYMSRCLELALNGMGSVAPNPMVGAVVVHNGKIIGE
                     GFHAQYGEAHAEVNAINSVRDKSLLQDSTLYVSLEPCSHYGKTPPCTDRIVEYGIPRV
                     VVATTDPNPRVSGRGLEQLRKHGVEVSLGILEDEALLLNRRFITFHTCKRPYIILKWA
                     QTIDGFIDLVRTAETPVGSVWITNELARSLVHRWRSEEQAVIVGTNTVERDNPRLNIR
                     SWSGRNPIRVIVDRKLRLSRDANVFDGSQPTILFIGNNSSALVRKSEFAGISNLEMVT
                     IDFAKGSEQQILKELSDRDVISLIIEGGATLLNNFIQKNLWDEARVFVGNRFFGEGVK
                     APLFKGDPVSYEEVGDSKLFIYRNQQGRSNR"
     gene            complement(21106..21681)
                     /locus_tag="PK839_02165"
     CDS             complement(21106..21681)
                     /locus_tag="PK839_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HOG71571.1"
                     /translation="MRISVYCASSSKINQKYFDATRQLASELANRGDIIVYGGGSHGL
                     MGCLADTALMNGGMVVGILPRFMNKVEWGHKNLTELKLVKDMHERKKLLIKDVDAVVA
                     LPGGCGTLEELMEVITLKRLGKFTKPIIILNTDGFYDYLKLLLAKMIDEHFMRPEHAG
                     IWTFVETPQEVIPAIEKAPTWDSDAIKFAAL"
     gene            complement(21944..22264)
                     /gene="trxA"
                     /locus_tag="PK839_02170"
     CDS             complement(21944..22264)
                     /gene="trxA"
                     /locus_tag="PK839_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005935455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOG71572.1"
                     /translation="MAQEVNDSNFDEVVLNAGKPAVVDFWAEWCGPCRMIAPYIEQIA
                     DEYKDRAVVAKVDVDNNPGIAARFGIRNIPTVLFFKDGQLADKQVGAVPKSNLVSKLE
                     ALLR"
     gene            complement(22340..25960)
                     /gene="dnaE"
                     /locus_tag="PK839_02175"
     CDS             complement(22340..25960)
                     /gene="dnaE"
                     /locus_tag="PK839_02175"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010992125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOG71573.1"
                     /translation="MGSFVHLHVHSHYSILDGASSIEMLVSRAKELGMPGIAITDHGN
                     MFGIKEFLNTVKKSNSPVLKEIESIKAEIKGISSGSSVADNSPSLDLLNEQLREAEKQ
                     LFKPIVGCETYLAKNSRFDRTDKEDRHNFHLILLAKNLEGYHNLVKLVSYGYTEGFYY
                     KPRIDKELLRQYSKGLIASSACLGGEIPQAILNNNIAEAERLIMEYRDIFGDDFYLEL
                     MLHKSGEPRIDSEVYENQLKVNKALLELSVKTGVKCITTNDVHFVMSDDAASHDLLIC
                     LNTDKRIDDTNRMRYTFQEYLKSEEEMLSIFSENPEAVHNTMEILDKVEFYDLSHKPI
                     MPNFPLPDGFTDDNDYLRHLTYEGAKLRWGDTLSDEIGSRIEFELQVIERMGYPSYFL
                     IVWDFIRAAREMGVSVGPGRGSAAGSVVAYCLKITDIDPIKYNLLFERFLNPDRISMP
                     DIDIDFDEDGREDVLQYVVQKYGEKRVAHIVTFGTMAAKSSIKDVARVLNLDLSTSER
                     LSKLVPERIGITLPAAIKEVPELEKELKSDNELVRATLKHAQVLEGTIRQTGVHACGI
                     IIGRDDLENYIPLCINKEAKLFVTQYEGNHMEDIGLLKMDFLGLKTLSIVKDALQLIM
                     ESTGKSLDIDNIPLDDSTTLELFSRGDTTAIFQFESAGMKKYLKALEPNRFEDLIAMN
                     ALYRPGPMEYIPSFINRKHGREKIEYDIPEMEEFLKDTYGITVYQEQVMLLSQKLAGF
                     TRGEADSLRKAMGKKLRDVMDKMKGKFIHGCINNGHDQAKVEKIWTNWEAFAEYAFNK
                     SHSTCYAFLAYQTAYIKAHYPGQFMAAVLSRNLSDIKKITTFMDECRRMGLQVLGPDV
                     NESNYKFSVMPNGDIRFGLGAIKGMGENAAANIIETRNHEGKFKNIYDFMERVNLQTV
                     NKKNLESLVLAGAFDNISPISRGAYFTPDSKGVTFMETLMRYGAKVQLEKNSKQMTLF
                     GATNGFEVPQPEPPKTDDWPTIEKLNKEKEIIGIYLSAHPLDEFRFEIENLCNTNLAE
                     LKNLEDFNNREVTIAGMVVKAKIDTTKNGKPYARLTIEDYSDTMDIMLFGKDFETYRN
                     FCYTGYMVMIKGKVQPKAFKPEELELHIKSMSMLYDVREKQIKGISITLPTTEINDIL
                     IDDFTRLTHENAKVSLRFKVVDVENRISVDLFSRSHRVSINTELIHFLTDNELEYKLI
                     TS"
     gene            26286..27338
                     /locus_tag="PK839_02180"
     CDS             26286..27338
                     /locus_tag="PK839_02180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG71574.1"
                     /translation="MRGKLLMLLVASGVILSVSAYGQNNLPAYLGVSYIPELRWKVYF
                     INHKSIGEPLSLNFDMTSMSSYEGNFGIRRLGVRMGVSAQTENNLIGKFYRWGGYLGF
                     KNMLLRLQSSKMSGDLEWSGALPPGYYANREFSNKYFNIDLLKTAKGGSAFNEVGGYW
                     GVGYTSMGYPVEISTLVTEGGRENQKFGMPAYDTLYSIKSYNICFGFDLLRQLCLTGG
                     LEGFAPGRRAMRFAMYASTQDKVGFGPAKISDYGVSMAEALNPGRTAVTSKFFNVMVH
                     YSLSLGLRYYIHPGPTVVIFALGYDFEGAMNAPFKGAADNAEDLGFDFLNYYLNHGVS
                     FKIFLAWNRDWQQKNQ"
     gene            complement(27347..28381)
                     /locus_tag="PK839_02185"
     CDS             complement(27347..28381)
                     /locus_tag="PK839_02185"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOG71575.1"
                     /translation="MKKLLLLSPQLVLTLSLFGQTIITSQNHGFIPNDNNPMLLTSYV
                     EPGPAGKNVVWNFSMLETSENFQGSLKEVYTLKDYPSNQGGNVVLEEFGNRFIFNMTA
                     NSLEQVGFQSSTGSTEITYSKPFVKMRYPFGFNSSFSGDFKGDYLVNEVPVGTISGTY
                     RVEGDGYGKLILPGGRELSGVLRVKEVKLTTQTINNSLINIEDVTYRWYVANHRFPVL
                     SLIKSTYTPANGKPSTQTKAAFNGNVVSQPSDVDKVSDNLLRFTTFPNPYKGKVAITY
                     TLPESCKVNLAVYDITGKLVSVLVNGIEDAGQKTHYFSAAEVGMPAATYIVKLTAGKV
                     EFTGKVLEVR"
     gene            complement(28734..30932)
                     /gene="recQ"
                     /locus_tag="PK839_02190"
     CDS             complement(28734..30932)
                     /gene="recQ"
                     /locus_tag="PK839_02190"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008156887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HOG71576.1"
                     /translation="MNVNTSELSLKEYLNKYFGFNTFKGNQEAIIHNVLTGNDTFVLM
                     PTGGGKSLCYQLPALILDGTAIIISPLIALMKNQVDSMRAFVLEEGIAHFMNSSLNKT
                     QLQKVRQDVTNGVTKMLYVAPESLTKEENIQFLKQIKISFYAIDEAHCISEWGHDFRP
                     EYRRIRPIINEIGTAPIIALTATATPKVQHDIQKNLGMLNASVFKSSFNRPNLYYEVR
                     PKVNAVKEIIKYIKSNQGKSGIIYCLSRKKVEEMAEILKINGIKALPYHAGMDAATRS
                     ENQDKFLMEEVDVIVATIAFGMGIDKPDVRFVIHYDIPKSLEGYYQETGRAGRDGGEG
                     QCITFYSYKDIQKLEKFMQGKPVSEQEIGKQLLLETVSYAESSMCRRKTLLNYFGEEY
                     DEENCNSCDNCLHPKTQFEGKEELQMVLETVLAVNQKFKADHIVNILLGNVTAAVKSY
                     KHNQLDTFGEGAEKDSRFWNSIIRQALVMKFLDKDIENYGLLFITPKGQEFIDKPTDV
                     LFTLDHEYEEADDDESIGMPMDRSGAVDDELLAMLKDLRKKMAKKKNLPPFVIFQDPS
                     LEDMAIQYPITIEELHSITGVGAGKAKRFGQEFVELIKAYVEDKDIIRPQDMVVKSVA
                     TKSLLKVYIIQSIDRKMDLEDIANAKNLEMDELLNEIEAIVNSGTKINLDYYINQVID
                     EDKQEEIFDYFRNEAETESLEEALVALGDEDYTEEEIRLMRIKFLSEMGN"
     gene            31085..32080
                     /locus_tag="PK839_02195"
     CDS             31085..32080
                     /locus_tag="PK839_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HOG71577.1"
                     /translation="MTKVANEREKMNNDAIRKLAVETIRTEADAIEKLAGFIDDDFVM
                     AVNLIFNGKGRVIVTGIGKSAIVAEKIVATFNSTGTPAIFMHAADAIHGDLGIIQPDD
                     VVVCLSKSGSTPEIKVLVPLIRNLGNKIIAVVSNTDSYLARSSDFVLKATVDREACPN
                     NLAPTSSTTAQMVIGDALAVCLLKLRGFTPADFARFHPGGALGKKLYMRVSDIFTSTD
                     VPKVRPDDRIRSVILEISSKRLGATAVVDSNDRVVGIITDGDLRRMLQKNQSIEGLAA
                     ADIMTVNPKTIDREELAITAFNLMEQNKITQLLVTHEDRYEGVIHIHDILREGIV"
     gene            32094..32894
                     /gene="tatC"
                     /locus_tag="PK839_02200"
     CDS             32094..32894
                     /gene="tatC"
                     /locus_tag="PK839_02200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HOG71578.1"
                     /translation="MAGKASKHNEMTFWQHLGELRNIFIRCMLAVVVLSIVAFCLKDI
                     IFQQVVLRPRMPEFVTNRLLCRLAEQWNSPVLCINSTILPLINIELAGQFTVHLRVAF
                     WSGVIMAFPYIMYELAKFFRPALKQGESAMLRGVALMFTLCFAIGAAFGYFIIAPISI
                     HFLGTYSVSAQVVNQITLGSYLSTVLGLIFAGGAAFEMPVVVLYLSRLGVVKPQFLSR
                     YRKVAIVVILVISAVITPPDVFSMTIVAIPLLLLYEVSVLLARMEFKD"
     gene            32906..35356
                     /locus_tag="PK839_02205"
     CDS             32906..35356
                     /locus_tag="PK839_02205"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HOG71579.1"
                     /translation="MRRLLFFLALQLAFVSSIFAQGQITKIRGRVIDAQTREPIAYVN
                     IAFRGLPIGTITSEKGEFFLETRTTGDSLTFSYVGYQSKTVAVKKGYFQEINVELQPE
                     AIELASIVVKPGEYQANRIVKNIIRNKDKNNPANLSYFCHSYNKIQVDLNNVNLEASQ
                     RKFLNQFKFIWDYVDTNAITGKPYLPIFITESLSDYYYRSSPKMEKEIIKATKISGVN
                     NESVAQFTGMIYQNVNIYDNYINIFDQGLVSPIANAGLIYYKYILVDSAFVGNRWCYQ
                     ISFKPRRKMEPTFTGDFWVNDTTWAVVKAQFRLSDMVNLNFVNDMVTTSEYVPINDTL
                     WFPKQLTMFVDFNLTDKEAGFFGHKTVTYSDVTLNPDFPSEITEQPTSLKVMEGALNQ
                     DKEYWNKTRPFELTPKEANIYAMVDSVQQVPLYKTFVDIINMIVNYYYVVGYVEIGPY
                     YQTYSFNEIEGNRFKVSGRTSNEFSTKVMLSGFLAYGDKDNRFKWGVGSMYMIGKNPR
                     QVLSLDYKSDIEQMGQSPNALTEDNILTSFLRRNPNYKLTMVKDFSVSYEKEWFTGLS
                     NTIYFSNRIMYPTEYIPFLPVGGGLQLKNITTSAVTLNTRWVKDETFVQGEFTRVSMG
                     SKWPEVNFDITKGIKGVLGSDYSYWKLHLNYYHKFNVNPFGYARVIADAGKTFGEVPY
                     PLLQLHEGNETYAFDRYAFNMMNYYEFASDQYASLYLEHHFQGFFLNRFPLLRRLKLR
                     EVATAKGLIGSISNSNMNVLQFPEGLGSVSKPYVEVGVGVENILKVIRIDAIWRLTHL
                     DNPDISPFGIRVGLQIIF"
     gene            35405..36130
                     /gene="lptB"
                     /locus_tag="PK839_02210"
     CDS             35405..36130
                     /gene="lptB"
                     /locus_tag="PK839_02210"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010265847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HOG71580.1"
                     /translation="MRLFTENLVKRYKTRTVVKGVSVEVSQGEIVGLLGPNGAGKTTT
                     FYMIVGLVKPNEGKIFVDDVEITGEPVYKRAQRGISYLAQEASVFRHLSVEDNLKAVL
                     EMTKFSKEYQMEKVESLLTEFGLQKIRKSKGIQLSGGERRRTEIARALALSPKFILLD
                     EPFAGVDPIAVEDIQNIVAHLKDKNIGILITDHNVHETLSITDRAYLLYDGEILKSGT
                     SEELADDEQVRKLYLGQNFELRK"
     gene            36197..36278
                     /locus_tag="PK839_02215"
     tRNA            36197..36278
                     /locus_tag="PK839_02215"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:36231..36233,aa:Leu,seq:tag)
     gene            36510..36983
                     /gene="coaD"
                     /locus_tag="PK839_02220"
     CDS             36510..36983
                     /gene="coaD"
                     /locus_tag="PK839_02220"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HOG71581.1"
                     /translation="MSKVALFPGSFDPITVGHESLVLRALSIFDRIVVAIGNNSSKKS
                     YFDIDLRKAMIEKVFANEPRVEIICYDGLTVDICNKLNINHILRGLRTAADFEFERAI
                     AQVNRAMYPHIDTIFLLTAPEHTFVNSSVIRDILQHHGDATKFIPASINIYDYMG"
     gene            37084..38493
                     /locus_tag="PK839_02225"
     CDS             37084..38493
                     /locus_tag="PK839_02225"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOG71582.1"
                     /translation="MRNLVLSLLVFITLGVTGVLAQQALVYGTAADYAGTDLVFYTYS
                     DYITLTEVEIGRCRVKANGNFEGSVKVLETTYVFSHLGIYRIYFFAEPGKRYQLVLPA
                     REEKTEAQRLSPFFRETDLQVGVANISQSDINFLVNAFDIRFNAHFDQIVADTYRGKS
                     QNVDSLINVMEEKFKNEPNSYFKAYREYRYGLLRQVTLSRKSSGISNELFLNKPILYS
                     NPSYMELFNLVYDKYFMFFSRTSTGNAIFSTISQEKSLTQLKKLLATNTVLSNDTLME
                     LVILKSLYDEFFSSNFSRSALLTLLDSVYRTTQIPEHAIIAENIRSRITKLLPGFVPS
                     PFKLKDVNGHEVTLDQLEGKYVYLNFCTTTSYTCLQDFSLLEKIYRKYRKKLEIVTIS
                     ADMEKKDLESFLKTTGYSWTFLHFGEKPDVIKDFDVRAYPTYFLIGPDRKIILSPAPS
                     PREGFEQRFFELLRSRGEI"
     gene            complement(38490..39113)
                     /locus_tag="PK839_02230"
     CDS             complement(38490..39113)
                     /locus_tag="PK839_02230"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOG71583.1"
                     /translation="MASEYVIFFNDRRFTITSDLAGYFVGNEKGYFLGHASSYDIPKL
                     VKIFQSHPAICNVFVGNGSVDKLFSWFTSQFKFLAAAGGLVRNNAGQYLMIYRRGKWD
                     LPKGKVEPSETPVEAGLREVEEETGISGLTVVRPLTCTYHTYEEKGNTMLKQTHWFLM
                     QVRGTLSLKPQVEEDIEIAEWVDADGVENRLKDSYPSIRMVFASLRS"
     gene            39094..39747
                     /gene="pyrE"
                     /locus_tag="PK839_02235"
     CDS             39094..39747
                     /gene="pyrE"
                     /locus_tag="PK839_02235"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018360455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HOG71584.1"
                     /translation="MTYSEAINAESIAANLLKIKAIRLSPTAPFTWASGLKSPIYCDN
                     RITLSYPDVRNRICNAFVELVRRLYPDVELVAGVATGAIAHGMLVADRLNLPFVYVRS
                     SPKSHGLANRIEGEYRREQKVVVIEDLISTGQSSIAAVEALKEAGCNVLGLLAIFSYQ
                     LEQADENFTSAGVELTTLTNYSELIGVAVSTGVVNASQLQLLMSWHENPESWGNITL"
     gene            39831..40355
                     /locus_tag="PK839_02240"
     CDS             39831..40355
                     /locus_tag="PK839_02240"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOG71585.1"
                     /translation="MPIIGTKYRLFIFVVESNPDCVARLMQGLEVPEQSLIKAFSSGE
                     SFIDFFPKSKIPKRKLILIFLGYTFSDEANQNMMNGIEILETVKMLNPAVHVVMLTHN
                     GEQEYGGHAKSMGANSCIMKDESMVIRANSLIRLLLSEKKLGNRRRQLKVAAVSWLVA
                     LVCFLLSVGVCCFS"
     gene            40549..42603
                     /gene="htpG"
                     /locus_tag="PK839_02245"
     CDS             40549..42603
                     /gene="htpG"
                     /locus_tag="PK839_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HOG71586.1"
                     /translation="MQTGKIGVTTENIFPIIKKFLYSEHEIFLRELISNAVDATQKLK
                     TLASVGDYKGEVGDTTIRVKLDKKAGTLTISDSGVGMTADEIDKYINQIAFSGAEEFM
                     EKYKNQANGIIGHFGLGFYSSFMVSDKVEIVTKSWRDDAGAVKWSCDGSPEFAIEEAT
                     RSSRGTDVILHISDESKEFLEESRISELLLKYCRFLPIPIAFGTIKEWKDGKEVDTGK
                     EKIINETNPLWVRKPVDLTEEDYMNFYRNLYPTQEDPLFYIHLNVDYPFHLTGILYFP
                     RIKTNFDIQKNRIQLYSNQVYVTDSVEGIVPEFLTLLHGVIDSPDIPLNVSRSYLQSD
                     SNVKKISSHITKKVADRLQEIFKNDREQFEKKWDDLKLFITFGMITDEKFYERAEKFA
                     LLKNVDGKYFTLEEYEKLVKDNQTDRNKNVIYLYASDREAQHSYIEEAKNRGYDVLLM
                     DGQLDPHFINTLESKLKDTRFARVDSDVVDKLISKEETREVSLSAEEQDDLRAVFEGA
                     LPEGDHYWVSFEELGETANPVIITHSEFMRRMKDMAAMSPTASFYDKMPDSHNLVVNT
                     AHPLVKAIVEAKNKQVGGKLKELNAGIEPLKVSVDELHKAQMEKKEEEIPQAQKEELA
                     EREKQLEELKEERRNLLKNFGREYKLVKELTDLAMLANNMLKGEDLSRFVKRSLELIK
                     TE"
     gene            complement(43114..44658)
                     /locus_tag="PK839_02250"
     CDS             complement(43114..44658)
                     /locus_tag="PK839_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HOG71587.1"
                     /translation="MSSNFFKTKPIQKILGEAGDGEHGFKKTLGPMNLTTLGIGAIIG
                     AGIFVLTGQAAAQYAGPAIVISFIISGLACAFAGLCYAEFSSMIPISGSAYTYAYATL
                     GEFLAWVIGWDLILEYLFAASTVSVGWSGYIVSLLYQIGVNIPPEFTAARGMELLQVP
                     ANIPGIDAGWLPKTGELVEFLANQGVNASSLPMVTSIINIPAMFIIAVISMLLIVGIK
                     QSANFNNIMVIIKVSVIVLFIALGFAFIKSSNLHPFVPERVGEWGHFGWGGILRGAAV
                     IFFAYIGFDAVSTAAQEAKNPQRDMPIGILGSLVISTVLYILVAIVLTGMVNYKFLNV
                     PAPIALAVDAMGEQYSWLRIIIKIAALAGLTSVILVMLLGQPRIFFTMAKDGLLPPVF
                     AKVHPKYRTPYLSTIITGAVAMILAGVLPISILGELVSIGTLLAFVIVCIGIIVLRKK
                     RPDLPRPFKTPWVPLVPILGAGICLLQMAALPFATWLRLIIWFAIGLLIYLFYGRNKS
                     KLNSGL"
     gene            complement(45228..47534)
                     /locus_tag="PK839_02255"
     CDS             complement(45228..47534)
                     /locus_tag="PK839_02255"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HOG71588.1"
                     /translation="MKCKLKIRQKILLYILGATGLFYIACVGYILITSRQTIYKEAME
                     KTQLTAEVSAKEVTAIFERELSLVRTLSQALTVYQELPQEQWHKLFLDMYLPVLRENP
                     QIYSLWDSWEYKYYIPGYTNDFGRFVITTWREGSQLKWLYDERSMEGDPPIYGVFKRN
                     GVEAIWEPYVDQVTKGKAEVKLMITYASPVYVNSEFAGAIATDVGLDNLQEMVSKIKP
                     VTGSYAYLVSNSGIIAGHPNKEFIFKKIADAYPADEKKYQLTERIAKGEKFNYTIKDS
                     SGREHLICYSPIVAGKVKTPWALVLSTPVDVIMAQARHTLWVSIIVSLLGLIAITLVL
                     LFVSGNLTRPIKKITEALKRLARGEISEELMVSINSGDEIEEMAVAFNTSLSGLHHKT
                     LFANDIGKGNYATQLELLSEADVLGKSLITMRDSLKNAAKEEENRKLEDQKRSWANEG
                     HAHFGEILRQNNNDLQKLCDNVIISLVKYVGANQGGIFLLNDDDKMHPFFELISAYAW
                     DRKKYMTKQIELGEGLVGACAMEKETIFLTEVPDDYVNITSGLGEANPRCVILVPLKH
                     EDEVVGVVEMASFKVLEKHEVEFIEKVAESIASTILSVKINARTKMLLEQSQMQAEEM
                     KAQEEEMRQNMEELQATQEEVSRKSGEIEEFMNSINSTAYVVEYDLGGNVINVNDSYI
                     RLVGVPREQIIGSHHTQNLILNDKQKKEYGTFWENLKAGVSKKLRTTITSGGRSIELL
                     ETYIPVRDVDGKVFKVMKLAFEVNEFQQ"
     gene            47693..50581
                     /locus_tag="PK839_02260"
     CDS             47693..50581
                     /locus_tag="PK839_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005927060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HOG71589.1"
                     /translation="MTLCSALVTFSMLGMAQDYKYQSVPGDPLNARIYTLANGLKVFM
                     SVSKDEPRIQTYIAVRVGSKNDPKETTGLAHYFEHMMFKGTPQFGTANWESEKVLIAK
                     IDSLFEVYRTKTDSLERARIYHVIDSISYEASKLSIPNEYDKLMTAIGSIGTNAGTSN
                     DYTIYMENIPSNQLENWAKIQAVRFANPVLRLFHTELETVYEEKNMSLTNDGRKASET
                     LLKGLFPNHPYGQQTTLGEAEHLKNPSMKNIREFYAKYYVPNNMAVVMAGDFDPDEAI
                     RIIDSNFGSLKPGNVPSLVYQPEVPFTQPKEFEVVGLEAENVIIGYRFRGANSPDNDM
                     LDLISAILYNGRAGLIDLNINKKQLTLGCQAYNRAMADYSILQFSGRNKKGQSLEEVK
                     NLLLQQVELLKEGDFPDWMLEAAVNNVKLRQMSQLESARGRASLMYSAFLNNIEWADE
                     VAYISRLEKITKEQVVRFANDNLGSNYVVVYKQQGKPEEVAKVAKPPITPIYINRDAE
                     SDFLKMIKNSTVKPIEPRFIDYDKDITKFKLNTNVEVLYTKNAENSTFNLTLYYPFGR
                     LANPLLTHAADYIQLLGTSKLTAEQLSQEFYKLACNFNISVRDEEMLISLTGLSDNAD
                     KAFELMENLLADCQPNSDALSMYITNQLKSRDDAKKRQQSVFSALVSYATYGEENPQR
                     LILSNEELKGLTAQPLVDLIREFTSYPHIILYYGPQDVKGLKKMLTEKHIQPKKYKSL
                     PVNKKFEETSTTTERVLFVNYDAKQSYLQTISKGELYSDNLYPVVTLYNEYFGGGMNA
                     IVFQEMREKRGLAYTARSLYSIPDEPNRVFMNTSFIATQNDKVIDAFNAFNELFNNMP
                     EAEIGFNLAKESLISNIRSQRIIRMALLYNYLDARRMGWNRDMRQTLFEVIPGMTLND
                     VRLFQQTHVKGKPKTYVILGKESDMDFNRLSSLFGPVTKLSLEDIFGY"
     gene            50743..52416
                     /gene="ggt"
                     /locus_tag="PK839_02265"
     CDS             50743..52416
                     /gene="ggt"
                     /locus_tag="PK839_02265"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HOG71590.1"
                     /translation="MLLLLALVYFTGSGQDRMTGLNFATRSEVIASHGMACTSQPLAT
                     QVAIDILKKGGTAVDAAIAANAMLGLVEPTGSGIGGDLFAIVWDAKTQKLYGLNASGR
                     SPKSLSLEYFKEHGIKHIPAIGPLPVSVPGCVDGWFELHSRFGRLPMKVILEPTIGYA
                     REGFPVSELIAYYWKGNAERLKQFEGFADVYLPNGKAPAKGEIFKNPYLANTLEMIAK
                     NGRDEFYRGGIARTIDDYMKRNGGFLSYNDLASHRSEWVEPVSTSYRGYDVWELPPNG
                     QGIATLEMLNILENFDIASMGFGSADYMHLFVEAKKLAFEDRARYYADPDFTDVPYMS
                     LLSKDYAKNRAALIDMNRAARSYDAGELQTGNTVYLTVADSEGNMVSLIQSNYRGMGS
                     GMTPTGLGFVLQDRGELFSLEEGHPNVFQPGKRPFHTIIPGFITKSGRPWVSFGVMGG
                     GMQPQGHVQVIVNLIDFGMNLQEAGDAPRIQHEGSSEPTGERMNDGGNVFLESGFDYQ
                     EIRKLLGKGHRISYSLDGYGGYQAIMYDERNRVYVGASESRKDGQAAGY"
     gene            complement(52465..53667)
                     /locus_tag="PK839_02270"
     CDS             complement(52465..53667)
                     /locus_tag="PK839_02270"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOG71591.1"
                     /translation="MLRKSVYALALGGLLLTACQKEENFSDETNPLSTKQELKNGDII
                     PGKFIVVFTQDTFSTRVDKVQQVSYEESHAKALAEASTFLSVYAVPSGKIDQVYGASI
                     KGFSAELNELQVNAIYRDSRVAYIEPDRWIVLDYKVESISDPTAKGQTTPWGITRVGG
                     ATNYTGTNVAWIIDTGIDLDHPDLNVDVSRSKTFVPRGRTPDDDNGHGSHVAGTVAAK
                     NNTIGVVGVAAGAPVVAVKVLDRSGSGAYSTIISGVNYVASAGKSGDVANMSLGGPTY
                     QALDDAVLAASNKGIKFSLAAGNESANANLSSPARVNGSNIFTISAMDNADKFAYFSN
                     YGNPPIDYCAPGVSIYSTYKSGKYATLSGTSMASPHACGVTLLGSPRTDGYVLNDPDG
                     NPDPIIHK"
     gene            53939..54439
                     /locus_tag="PK839_02275"
     CDS             53939..54439
                     /locus_tag="PK839_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HOG71592.1"
                     /translation="MVELVKGDITLMDVDAIVNAANESLLGGGGVDGAIHRAAGPQLL
                     AECRTLGGCPTGEARITRGYNLKARYIIHTVGPVWHGGSRGEAKLLASAYWNSLQLAA
                     DYSLKSIAFPNISTGVYGYPKREAAEIATRTVNEFKQQNLHPERVVFVCFDDDSYQIY
                     KEMLKR"
     gene            complement(54436..55818)
                     /locus_tag="PK839_02280"
     CDS             complement(54436..55818)
                     /locus_tag="PK839_02280"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOG71593.1"
                     /translation="MKEIRILVLDDEKVFRDEIKEFLENDGFTVLLSDRPSAAFEMLT
                     QQEVDIMILDIKLPEMDGFEVLRRVKEEYPDIEVIMITGHGDMEAVIQAMRLGAVEFF
                     PKPFRLLDMRAAIQRTKRYITISNQLKEINQTYSLISKELIESVGNEILGTSPAISHV
                     VNLMGRVAKTENTSVLITGESGAGKELVARGIHYLSNRKDSYFYAVNCSAIPESLFES
                     EFFGHKKGSYTGANEDKAGWFEVANGGTLFLDEIVEMLPAMQSKLLRVLEERKVRRIG
                     SSVDIPVDVRIIAATNQNVFTQLETGKLRSDLYYRLNTFEIHIPPLRERREDIPVLLD
                     YYVKFFARKLGKRISEIDPSVQRVMMGYNFPGNIRELRNMVERAIILCDGSKLSLKNF
                     PGLTSQTGEAGTQLPGIGDDVFDLDLIEKFVIMKALERTGYKKTEAAQLLNITRQSLD
                     RRLEKYSINI"
     gene            complement(55825..58068)
                     /locus_tag="PK839_02285"
     CDS             complement(55825..58068)
                     /locus_tag="PK839_02285"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG71594.1"
                     /translation="MSRFGKHTTIVLAFWLFMQFPTLQAQVSPDSSLTSKSEEKVLAN
                     LISEGRDNVVKNPDKALDNGLQALVLAKKLGDTRFEIEALLILAESNFTLGQYENSSR
                     YYNRALNFYDQLGNSDAKYKILIGLGRVYDAEKNPELAISTLERGLASDENINNINLY
                     IDLLELLGELYQKQGNYRKSAEMYSKVLAIVDNPMGRKVLLKEKIKSDAFFKLGQVKK
                     NSGELVESLSNFKQAAEISINNFDSLSYSNALQEIALTYYLLQKPDSANFYFTASYAI
                     SNKISDTVGMIQGLQGIADIYLDSGNPTQALYYYNQQLTVAQKLKNIPAITESLVKIS
                     RCYLSSGDISNASKYLNRALAIARQNNLTQSVADVYQYLAILNEGQGRYRDALEFYKM
                     WVETRDSIYFEETGQKLAKLKILYEISQKEKENELLRQNSEIQNLQLNKTRYQTRILI
                     LIIIIFITLIVLLIILFRSKQKEIAKRLETEQRITDLNRELERRMIQEIKKQEKQQLL
                     LAQKSKLESLGTLAAGIAHEINQPLGGISMGLDNILLKVQDKSYNDEFMKEKISSLFE
                     NVERIKKIIDHIRYFSRAQKPITFTQININDVVNSSLFMVSTQYENHGVKMEVTLDEN
                     IGSITADKYKLEQVILNLLSNAKYAVDEKGKKHANHQYTKLIEIKTWQDNNQVYLSVR
                     DNGMGIPDKIIDKIFDPFFTTKSEERGTGLGLSVSYAFIKDLLGDIKVESVEGEYTRF
                     EISLPKM"
     gene            58423..59283
                     /locus_tag="PK839_02290"
     CDS             58423..59283
                     /locus_tag="PK839_02290"
                     /inference="COORDINATES: protein motif:HMM:TIGR01475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA-like polyprenyltransferase"
                     /protein_id="HOG71595.1"
                     /translation="MVKTIRNYLSLVKFSHTVFALPFAVIGFFLAYRQTPQNVDVWLF
                     VLVVLCMVFARNAAMSFNRLVDRRFDAINPRTQNREIPRGIITSRATAVFVTVNVLLF
                     IATTYFINRTVFFLSPIALAIVLLYSYTKRFTMFCHFVLGLGLALAPIGAYLSVTPRF
                     ELLPLLYSVIVLFWVSGFDIMYALQDEDFDKRENLKSLPAALGVKKSLLVANLLHFVA
                     GAVVVLIGILYHSDVWYWVGAALFIALLIYQHLIVKPTDISRVNMAFATTNGLASIIY
                     ATFNVISIFF"
     gene            59344..59796
                     /locus_tag="PK839_02295"
     CDS             59344..59796
                     /locus_tag="PK839_02295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG71596.1"
                     /translation="MMKKVFLLAATAASILACSCGTKSSSDNQQAKADSTAASIVEVS
                     IDSLISNPQAFANQTVKFTAKVDHVCMHSGQKLTLMGSDSGSTFKVMATDAVPQFDQN
                     LNGTTVEVVGVVQGAAPEDVESCSEEEGGQQKAKKLDYAVFCQKVRAL"
     gene            59913..60455
                     /locus_tag="PK839_02300"
     CDS             59913..60455
                     /locus_tag="PK839_02300"
                     /inference="COORDINATES: protein motif:HMM:NF027675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HOG71597.1"
                     /translation="MRKLLRVLHRDFGYFFTGMTLIYCVTGILLNHTRDWNPYYSIDI
                     RKVEFNLPLDTSLINKELLTSKLEPIGVQGLRSFYYPKPGTVKLFVSDGTVTSDLGSG
                     SALVERSYRRPVLYQLNMLHKNLPKSWWTWFSDIFAIALMAITISGLFLVKGKNGITH
                     RGVILIIAGLALPLVAIILL"
     gene            60714..62090
                     /locus_tag="PK839_02305"
     CDS             60714..62090
                     /locus_tag="PK839_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HOG71598.1"
                     /translation="MELPFAESYKIKMVEPIHRSTRSERERWIKEAKYNMFNLKSEQV
                     FIDLLTDSGTGAMSDRQWSALMMGDESYAGASSYYNLKSAIQNILGFEHFLPTHQGRA
                     AENVLFSVMVKEGDYIPGNSQFDTTKGHIEFRKAEAVDCTIDEAENTEIYHPFKGNVD
                     PQKLDDFIRTHADRVPFVIITITNNSAGGQPVSMQNLKDARKICDKYGKPLVIDSARF
                     AENAYFIKVREKGYADKTIKEIVREMFSYADAMTMSSKKDAIVNIGGFIAMRSKELWQ
                     QCSTFNIMFEGFVTYGGMSGRDMNALAVGLDEGTEFDYLETRIKQVEYLGNRLKDFGI
                     PFQFPAGGHAIFVDANKVLANVPREQFRAQTLAVELYLEAGIRGVEIGALMSDRDPVT
                     REVRMPKLELLRLAIPRRVYTNNHIDVVAVALKNVFDRRLEINKGLRITWEAPIMRHF
                     TVQLDRAR"
     gene            62201..63559
                     /locus_tag="PK839_02310"
     CDS             62201..63559
                     /locus_tag="PK839_02310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOG71599.1"
                     /translation="MRDFTTGSETQHILRFATPMLIGDIFQQLYNVVDSIIVGRFIGD
                     KALAAVGASFPVIFVIIALVLGIGIGSTVVISQYFGARNFDMVKRAANTIYIFLLISG
                     VVVTAIGLMASGAIFRMLHLPEELMKPARTYLNIFMLGMVVMFAFNAIASILRGIGDS
                     KTPLYFLLISTLTNVGLVLLFVVKFRWGIAGAAWATVISQVVAFIIALIYLNHSQHML
                     SINLRTMKFDRQIFRQIVKIGLPTGIQQTMVALGSMAMMGIVNSFGTTVIAAFTVASR
                     LDALIATPIMDFAAALSGFVGQNIGANKIDRVKRGLRSTLKISISFCVVNIAIILIFG
                     HSLMGIFTKSSDVIAIGYQYLLVVSLFYIIFAVMFSINGLLRGVGAAVVPMYITLLSL
                     WIFRIPLAYLLSKTFGMGALGIWWSIPIGWTMGAIAAVAYYRHGSWKNKSLVKVRQQA
                     AD"
     gene            63952..64611
                     /locus_tag="PK839_02315"
     CDS             63952..64611
                     /locus_tag="PK839_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG71600.1"
                     /translation="MLKDYLSRIQDTINKGDAREESYYSHLDSLIKEFAIENGIKKID
                     VTILPKKTEAGNPDFRVWDGKNHITGYIEAKDPSVDNLDRIEESEQLTRYRSTFPNLI
                     LTNFYEFRLYRDGELVRKVTIGRQRIAQKLKTAPPVENEKEFNDLLGTFFSFSLPQVR
                     SAKALAQELAKRTRFLRDEVVSIELAEEGRIGKRDIMGFYEAFRKYLIGTLTEQSFAH
                     L"
BASE COUNT        17034 a        14268 c        15193 g        18160 t
ORIGIN      
        1 ggttgattag ggtgtcgaaa tagccatcgg gttggtttat aatgttgaat ggggtagtgg
       61 tggacactat cagcgtttta tcatcaaata atcgggtgtt ggtattcagg tactcgtaaa
      121 cctcgttccc aaattctgct tgtaacgttc gcttaatcag ctcaaccctc atggggttgg
      181 gtgccctggt tgtttggatg ggcggggtac gacgctttac gctccgctca atcatcccca
      241 gcaaattatc ggggataacc ctgattcgct ttaatccggc ccaaaggttg tatatgaata
      301 gctccagcag ggttaccatc agaatggtgt tgaacacgat aaaccgggag tacctgtcgg
      361 accgggtgaa gtatatgaga aaagttacaa cggcaaatgc caggaggttt gatttaataa
      421 tatttgctga tatttttcgg gccgaatggg ttgtaaacac atacttctcg aaaactattg
      481 acgttgcaat ccatatcagg gcaaagagca gaagtgcatt ctggtagctc tccaggtagc
      541 tggcaaaact tacccctttc agcagtaccg ataggtagaa agctaccagt atcaacatca
      601 ggtctataat tatcagtatg aaaatacgct tgttcatgtt ggcttagcct ttgtaccttt
      661 ttaaatccaa tttacctgct gcaaatggcc agcagatttt taaaaaatgt aaaattacct
      721 ttaaaatata accttttgac ctaaaagagg ttcaattttt tatgaatcat gctattttga
      781 atgttaaggc aatgatacat tataccaacg ccatgaagga accccacgat tttctgataa
      841 gcaatttcat tccaatttat taacgcgttt ttgggtaaat ttgccattag tttaaatttg
      901 gtggtgctta tgagagtgga taagccctcc ttaacccatt agcgaaagaa agagttgaag
      961 gtaaacggag ttaactatcg gacgatatgg ctggaggatg gcagcagccc atgcgttaaa
     1021 ataattgacc agcgatacct gccccatcgg tttgtagttg agcctattga aagctttgat
     1081 gggttactac gggcaatttc cgaaatgcac cttaggggag ccggcttaat tggcgtagcg
     1141 gcagcctttg ggatctacct tgcagtgtgg gagcaaagaa accttgtcaa ttttcaggag
     1201 gcgttggaga agtgtgcctt aaagctgatt aaaacacgtc ctactgccgt aaacctgaga
     1261 tgggcggttg aaaggcagct ggaagttctt gcaggggttg agaatcccga cgatagggtg
     1321 gccattgcac gtcaaacggc tatagatatt gccgaagacg atgctggttg ctgtcggaaa
     1381 attgggctgg agggagtagg tttaattgag gacattgccc gcaacaagaa tggcggagtg
     1441 gtcaatattc taacccattg caatgcagga tggctggctt ttaccgatta tggatcagcc
     1501 ctggctcccg tttacgaggc atggcgaagg ggtgtaaggc tgcatgtttg ggttgacgaa
     1561 acccggccac gcaaccaggg tgcatcgctt actgcatggg aactgctcaa cgagggaatt
     1621 acccacacgg tgatagccga taacactgga ggacacctca tgcagcatgg gttggtggat
     1681 ttggtgttaa ccggtgctga ccgtatcacc accaacggcg atgctgccaa caaaattggt
     1741 acctacctta aagctcttgc tgcccacgac aacggggttc cattttacat tgcagcgcct
     1801 tcatccacct tcgactggtc gctcagcgac gggctttcgc agatacccat cgagcagcgc
     1861 catcccgatg aggtgcggta cgttggtggc tggctggatg gcaggcttca aaacgtgctg
     1921 gtttgccctg atgaaagccc tgccgccaac tttgccttcg atgttacacc cgcccgatta
     1981 atttccggga ttattaccga acggggaatt tgcaagccct tacctgctgg cataaagaaa
     2041 gtttttcctg aaaaattcaa ctattgaacg agaagggcat tattaaattc aggtgcagct
     2101 gggttaacgc cgaccctctt ccctacgaag cggttgccga cttgaaccgt tggcgttgca
     2161 tactctacaa caaaggtctg gttggagtgg attgtaacgg ggttggtttt ggaaacctga
     2221 gcaagcgtta ccttcacaac cagttcgtca ttacaggctc aggaacagga caacttaccg
     2281 agcttaccac catgcactac acgctggtta cagatttcag cattgggcgt aactgggtgg
     2341 aggccaaagg cccggttatt gcttcttccg aatcgcttac ccatgccgca ctttaccagt
     2401 gcgataaggc aattaaggcg gtgttccaca ttcacaacct tgaactttgg aagcggatta
     2461 tcgggaaagt gcccacaacg ctgtctaatg ccgagtacgg gactccccaa atggccatgg
     2521 aaatcaaaag gctatacaga caaaccgatc tccgtaaaac cagagtgctg gtcatggctg
     2581 gtcaccagga gggaattatg gcctttggcc ttgatgccga tgaagcaggg aagcgtattt
     2641 tatcgctcat gaaggaatac aacccataag ccaagttttt gaggcagtgc tataaacttc
     2701 gcggataaat ctttactacc taccttccat tctttccgtc ctactaaaac aatgaagttc
     2761 agttgtcgca tagcagctac cccaaacttt agctcagggg cgattggtta atttttccat
     2821 taactaaaaa tttgctgagt aggcatacac aggctttacg cctggctgat tagctcaaac
     2881 aacagatgat ttttccgcac gagctcggca acattgacaa acccactaca gctacacttt
     2941 tgtttttttg cttcatattt tatgcttgtt ttgataactt tttataagtt tgagttttaa
     3001 aaggtggaat gcctgtttgc agagcaaatc tgaaaattca acttttagta gtaatcatct
     3061 aaaaaacctg tatcatgaaa agttttttta aatacttgct tgccaccata gttggcgtgc
     3121 tgctgtcgtt tcttctgata tttttgataa tgatgggtat tgccggcagc atggtttcat
     3181 cagccgataa agctgttgag gtaaaggaca actctattct ttacatggat ttaaaaaacg
     3241 agattgttga tagggcaagc agcaacccct ttgaaacact cgacctggca actttcgaac
     3301 cccagaaaaa ccttggcctt aatgagatac taaaagcaat tgccaaggca aaggatgacc
     3361 caagaattaa gggaattttg ctggagtttg acgtggtcaa tgctggtgct gcaacaaccg
     3421 gcgaaatccg tgaggccctg agggaattta aggagtcggg taagtttgtt gttagctata
     3481 gtgataccta tagccaaaaa gcatactacc tggcctctgt agccgacagc atctacctta
     3541 accccgaggg gatggtggag tggctcggcc tgcgttccga aattatgttc tacaaaaaag
     3601 cgtttgaaaa gctggatgtt gaaccacaga ttatccgtca tggcaagttt aaaagcgctg
     3661 ttgagccatt catgagtgaa aggatgagcc ccgagaatcg cgagcaaact cttacctacc
     3721 tttcctctat ttggggcagt tgggttgatg aaatttcaaa atcccgtaac atagctgttg
     3781 cagagcttaa ccgcctggcc gacaacatgg aggtgtacga tggtaaatcc tgcatgaagt
     3841 cgaagctggt tgacagcctg atatacaagg atcagctgtt agagaagctt aaggctttta
     3901 caggaatacc cgaaaatcgt aacctgaata ccgttacctt ggagaaatac ttcaaggtgc
     3961 ctcccgttca aaaaacggct ttctccaaaa acaaaatagc tgttatttat gcccagggag
     4021 agattggcat gggtgatggt agcaatacgg caattggctc ggaaggtctt tcgaaggcca
     4081 ttcgtcaggc taggcgcgat agcactatca aggccattgt tttccgcatc aactctcctg
     4141 gtggtagtgc tcttgcctcc gaggtgatat ggcgagaagt tgagctggcc ggacaggcta
     4201 aacctcttgt ggtatcaatg ggcgatgtgg ctgcttcggg tgggtactat attgcttcac
     4261 ctgcaaccac cattcttgcc agtccaacca ccataacggg ttctattggg gtcttcggcc
     4321 tattcttcaa ccttcaaaaa actttagata aaaagttggg tattaacgtg gatgtggtga
     4381 aaaccaacgc ccattccgac tttttctcca tgtttcgccc catgactgcc gaggaaaagg
     4441 ccgttgggca gcgttttgtt gaacaaacct accagacctt tatcgggcat gtttctcagg
     4501 gaagaggcat tcccgtggaa agagttgacg agatcggcca gggtcgcgtg tggagcggtg
     4561 caaatgcaaa agaaattaag ttggtagatg attttggcgg gctaactgca gccataaagc
     4621 tggccgccga aaaggcaaac ctcgacacat accggttggt ggagctgcct aagcagaaat
     4681 cgcccttcga ggagctactt aaggggttta caaccgaggc taaagccatg ctggtgaagg
     4741 acgatttggg caagggatat aagtactaca acagggttat gcagatggtt gaaaaccagg
     4801 gagtgcaggc tagaatcccc tatgatgttg aagtttacta gaatgttgcc attcatgcac
     4861 ggaaaatagg catgaaaagt gttgagcggc ttagggttta ccctgagccg tttggctttc
     4921 aacagcatag ctaccaacag aagtttgtta gctggatgtt agaagtttaa ggcacaaccc
     4981 ggttagcatg ggtaacgacg tttggaacgc ttgctcgtca atcctgaaat ccggacgatg
     5041 cagctgcagg gttggagcgc ctggcgtgcc aacgccaatt ctgaaaaaca ccgatgggat
     5101 tgcctggctg aagtaggcaa aatcgtcggt ggtcatcctt ggctccaggg gagcaacgtt
     5161 ttcggaaccg aaaatactgc ttgcaacctc aacggctgtt tgggtaagga ttgggtcgtt
     5221 gtaaaccgac gggtagccct tgcgaatttc cacagagcat tctcctcccg tagatctggc
     5281 aatatcactt gccatttgga caattaaatc gtgtgccttt gccctccatt cctcgttcat
     5341 tgttctgaaa gttccggaaa tgcttacctc gtctggtata acattagttg agccatttgc
     5401 cgttacccgg ccaaacgaca aaacggatgg gatgttggaa ggtgccttgc ggcttaccag
     5461 tgtctgaagg gataaaataa tcgtcgaggc aatgactatc ggatcaacca gggtgtgggg
     5521 taatgcggcg tgtccccctc gtcctctagc cgttagataa acctcatcgc ccgatgccat
     5581 gtagcgtcct gggcaaaagc caaccttccc ggtggtaagt tcgggtagca cgtgcatgcc
     5641 caccatatag tcgggtttta cctcctgaaa ttcatccgat tcgagaatgg ccctggcacc
     5701 accgggaagt tcctcctctc ccgattcgaa aaccagcacc acttttccac tccagaattc
     5761 gcgtaggttg ttgaggatta ttgcggcacc tagaaccgac gcaagatgaa aatcgtgccc
     5821 acaggcgtgc attactcctg cggttcgaga ggcgtacgat tctccggttt tctcatcaat
     5881 gggcagtgca tccagttcag ccctgagtcc aatggttttg ccttcacctg tgccgtcaat
     5941 agttgccaaa aggctgtagc tcttatctag ttttattatc cctatgccta acgattccaa
     6001 aaagctggca acatagcttg atgtgcctga ttcgcggaac gatagttcag gattttggtg
     6061 gatatgccgg taaatatcac cagcaacaac cggagtagtc ttgtagagtt cctttaactt
     6121 gtcatttagg ctggccatca ttttgcaacc tggttaaacc attcaaactt acatgtaatt
     6181 tccgaaaaaa tattcatcat cagcactttt acggacgaaa accttacatt actggctaaa
     6241 gccagatgtt tagatcattt ccctcaaagc ctttttgtca aaatttggtt tactgtttga
     6301 ggctcgcact tttttacttt gaaaaaattg ttgtagttgc ctgtaattcg taactttgat
     6361 gttgatttgg tttactacct gccaggtgaa aatcgttttg cttgcagtat tttttagctt
     6421 ttgaaaatat tgagtattga tattgctgaa gtatttttac ctggttttcg ttttgaagga
     6481 cttgaacctg tgtgagttct gcttaccagg tatacatgga taagcctaaa gcataactat
     6541 ggtttgtttt gaatttgaga ggttttgagg cagtcaagtt acaacatact aaatgtagat
     6601 aatgatgaaa aggattttga tttccggttt tgcctgtgca atgctgctaa taggtattgt
     6661 tgatgctcag tcgcaggtgg ctaggattta ctttaaagat tccaatcacg attacggagt
     6721 gataaaggag gacgatggcg ttgcatacta cgagtttcag ttttccaaca caggaagtac
     6781 accgctaata attcagcggg ttgaaacctc gtgcggctgc acaacacccg attggccaaa
     6841 agaacccatt gccccaggta aaaatggaag aattaaggtg ggatataacc catcggggcg
     6901 tccgggggcc ttttccaaaa ccattactgt ttactcaaat gccgaaacac ctacggttat
     6961 tctacaaatt aaaggggagg ttattcccca tgttaaaact cccgatgaga tattcaacat
     7021 ttccatcggc ggtaagctat actttgaaaa aacccatatt ccttttagcc ggctattccc
     7081 cgggaaaatt tataccgaca ccctcaaatt tatgaacaaa tcaagcgagg tggttaaggt
     7141 tgctgccaat gcaggtgggt taaatttctt aaccgtaagg gttgagcccc agagtattaa
     7201 gcctaaacaa gttggaaata ttatagttac ctacgatcct caaaaacgaa acgactgggg
     7261 ttttgtaatg gacaggttta tgctttctat caacgattcc ctgattacaa acaccccaat
     7321 atcgattacc ggcagcattg aggaggatta ctccgccctc agcgccagtg agctggaaaa
     7381 ggctcccagg gtagaattta acacaacctc ctacgatttt ggccagaagg ctgagggaga
     7441 acccgtagag tacagcttta ccattaagaa cacagggaaa agcgatctgc agattcgtaa
     7501 ggttaaagcc agctgcggct gcacaaccgt tcaaccttca aaaaacattc ttaaaccagg
     7561 cgagtcaacc gagattaaag ccctgttcag gactaatggg tattcgggta ggcagagtaa
     7621 atctattacc gtgataacca acgatcctaa aagttcaacc acggttctgc gtttatcggg
     7681 aaatgttgtt aagaaatagt aagctgcatt gatgtaatga tgcggaaaaa gggctgcaac
     7741 ttgcagccct ttttcgtgag attaagccac cattcattcc atctgctcgc aattattttg
     7801 accttccccg ttgttcgctc catgcacggc atcacttcgc cagcttacat ttgctgctgg
     7861 caaggggcta ccgaacaagc ctgccattac atgtttctta acatgttttt cagaaaacat
     7921 cactaagtat aagaataaca gacatttgac ttgaattttc attttttgtg aaaaatacga
     7981 acctaaaaaa aaattccaac gaaaaataat tatttcttaa tgcacttaat tttgtgagcg
     8041 ttatggattt taacaaacct taaatttagt aaacacttta aaggaggctt tatgaataaa
     8101 cgtaatgtag taattattgg cgctgccgga agagattttc acaacttcaa cactttcttt
     8161 cgcaacaagg aggagtacaa cgttgtagcc tttacagcag ctcagattcc cgatattgat
     8221 ggtagaaaat atcctgcaga gctggcagga aagctttacc caaatggaat tcccatagta
     8281 tcggaggagg agctaccccg cctgatcaag gagaagaacg ttcatgattg cgttttctcc
     8341 tacagcgatg tcaagtatca gaatgtaatg cggatcagct caattgtgaa cgcagcaggt
     8401 gcaaacttta tgctgttggg gcccaatgag accatgctga agagcactaa gccggttatt
     8461 gctgtggtag caacccgaac cggatgcggt aaatcgcaaa catcccgaaa ggttatagag
     8521 tacctgatgg ggcttggcct gaaggttgtt gctgttcgcc accccatgcc ctatggtgat
     8581 ttggttgccc aaaaggttca acggtttgct acggttgaag accttaaaaa gcataagtgc
     8641 accgttgagg aaatggagga gtacgaaccc cacgttgttc gcgggaatgt tatctatgct
     8701 ggcgttgact acgaggccat tcttcgtgaa gccgaaaagg atccaagcgg ttgtgatgta
     8761 atcctttggg atggtggcaa caacgacttt tcgttctaca aacccgattt gaccataacc
     8821 gttgccgacc ctcaccgtcc aggcaacgag gtaagctact atcccggcga agttaacctc
     8881 cgtatggctg atgtagttgt aattaataaa atggattccg ctgctcctga gggtatccag
     8941 atggttcgtg ataacatccg tagggttaac cccaaggcca ttgttgttga tggcgcttca
     9001 cccattaaag ttgataaccc cgagctgatt acaggcaaac gctgcctcat tgtagaggat
     9061 ggcccaaccc tcacccatgg cgagatgaag ataggtgccg gcaccattgc agctcgtaag
     9121 tttggggctt ccgaagaggt ggatgcccgt ccgttcctcg ttggaaagct taaggaaacc
     9181 taccagattt acccaaacat tggccggatt cttcctgcaa tgggctacgg tgagcagcag
     9241 ctcaaagatt tggaaggaac catcaacgct accgattgcg atagcgtgat tattggcaca
     9301 ccaatagacc ttaatcgcat tatcaaaata aagaaaccaa gcacccgagt ttactacgat
     9361 ttggttgaaa ttggtcttcc cgacctcaaa ggagtactcg agaaatttgt gaaacagcac
     9421 aagcttgata agtagtaagc agtatttttg aggtataatg gttaaggcgg ctcaacaagg
     9481 ctgccttaat tctttttagt gatgccttgc ggtgaaaaaa atcataccta aatgtggccc
     9541 atattggcca tttagagact aaagcctttt ctaaatcttg atcttggaga atgggaagtt
     9601 ttgtagccta ttctttgtca accaaacctg ttacagttct gtaaatctgg ttgttaagat
     9661 cgttaaccaa gtcgaactct tcagctttag cttttcggaa ccttcgtagc gataactggg
     9721 aggtaaatcg gaagaactcc cttcgccata ggtaaaaggc agcagcacca aggggtagcg
     9781 tggccaggta ggccaaagct agccaaatgt ttttcagcac aaatccaaca accagggttt
     9841 gtactagata tgccaggggg gttaaaagca acgccccggc atgttttata gagctgtgaa
     9901 actgaacatc cttgaacttt ttgctgagca ggtaggggac agctatggga atagcgttgt
     9961 ttaccgcccc atacagggca actggcatgg taattagcag caagagcaat ctgattggta
    10021 aatcccgaag ggtttttgag gaagcaggca ggagttttac ggaaagccca ttagcactta
    10081 ttatcctgcc ataatcattc gcatttttga ttatctcctc gaattccgaa gcatttgtat
    10141 ttttcagcct gtctagcagt gaaatgattt tcttatcaac ctgaaatttc tggttttgtg
    10201 acttaggtag ttttctctcc ttaatgtacc ttgctgaaaa tagctccagg atggtaaggt
    10261 aggcggtata gttttcatcc tctatgttga tcatttccaa cctcatgcgt tgctccagct
    10321 cggcaattag agcgttgtag gcctgtgccc tattctgcct gtagagctca aggtagcggt
    10381 caacctcaat gggttcaccc agcctgatta aaaggttgct tctgaaagct acgtagttgc
    10441 tgtactctag cccaacaggt actatctgaa taccaagttt accgttggcg gcatcctctg
    10501 cctgaaaggc aatacgggca attcctttct tcagctggcg gagcttttta accccctcat
    10561 ggcttccctc gggaagcacc accaacccat tacggttttg aaggacatcc aaggtctttt
    10621 ggaatatcct gtcgttgaga aggagatttt caaacccgtc gcgcgaacgg taaacgggca
    10681 taattttaag aaatgtgaga atttttgaaa ggaaaggctt tttgaatata tccgctctgg
    10741 ccagaaatat gggttgaaaa tcccttttca gcgtaaggat ggcaagggcg tccataaggg
    10801 tattctggtg gttgggggca aaaatcaggg tggcattatc tggaagtttc tcaatcccaa
    10861 ctatttcagt tctaaagtac agcttaaaga aaaaattctc gtggcacttg agcaaccagt
    10921 atgccagaga ccattgctca actttatctc ggttaaaagg cattgcgttt ggggtatata
    10981 attgttagcc tgcaaatgtg atactttttt ttataagttc catagagttt atggtaaagt
    11041 aatccgtgtt tggcattagt agcgtttgca ttattgagcg ctgacgagta tcataaaaaa
    11101 tgatttacag tagtatgaga gcaataaaat aaaaaagcat atctttgcgg ggcaaaaaag
    11161 taggtttaac caattaaaaa agcgttaagt atgcctgtaa aaatcagact aagccgccat
    11221 ggtaggaaaa aaaatcccta ctaccacatt gtggtggcgg atagcagggc gccacgggat
    11281 ggcagataca tagagaggat tggttcatat aatcctctga caaatcctgc tactattgaa
    11341 ttaaattttg atagggcatt ggactggctg caaaaaggcg ctcaacctac cgacacctgt
    11401 agggctatcc tttcatacaa gggtgttctt tataaaaatc acctgctcat tggtgtaaaa
    11461 aagggtgcaa taaccatgga gcaggcagaa gccaagtttg atacgtggct caaggagaag
    11521 gaaggcaaga ttaaggcgaa gattgacctc gtagaaaaca gcaacgctaa acgaaagaat
    11581 gaacgacttg aagccgaagc caaggtacgt gaggctagag ctcaagcggt tgctaaaaaa
    11641 cgagctgccg aggctgaaaa ggaagctaaa aaatcttccg agaattctgc tcaggatgcc
    11701 gtggaagcaa atgctgatac tgaggctggc agcagtgaag aatagtaatg catgaccgat
    11761 actcagcttg ttgaggttgg ctttattcag agaactcatg gtgttaaagg tgaaatccag
    11821 atttcagtag cgagttcagc tggcgtttta cccgaggagt tggaattggt atttcttgat
    11881 attgaaggaa taccagttcc tttttttatt cagggtatag ttgctatttc cgacgagatt
    11941 ttgattgtta agtttgacga agttgattcg gttgaggagg ctgctgagct ggttggcctc
    12001 cgtgttcttt taccctgtgg ctgtttatca aatgatgatg ggggtgttcc tttttcatcc
    12061 ctagtgggtt accggctatt cagtaccaaa aatgaggagc ttggcattat tacccgctac
    12121 gaggagtttg cctataatgc cctttttgcc gttacggcaa aatcgggaaa ggagctgctt
    12181 atccctgccg ctgaaaattt gatttcaaga atagatgatg ctgaacgaat tgtttggatg
    12241 gatatacctc aaggtttact ggaggttaac tccatggatt aggccttctt tctcaaaatc
    12301 agcaattctc tccctccaaa gtaaagggat cgggatggaa agtccactgg agttgctgaa
    12361 gcaaaccagc accgacttgc tcgtggtgta aacctctttt gataccctgt tcacaatctc
    12421 ctgtcccatt ttgaggcttt tagttcccat ttcgtagatt ctggtaaaca cctctatgct
    12481 atcgtaaagg gttatgggtt tcaggtaatc aatagtaatg ttggccagca ccaagccctc
    12541 tgtttccaag tttagatgtt ctcccagcac cctattaaag taggcaattt tccccagatc
    12601 gaagtactgt tggtaaaccg agttgttgac gtggttcagc gggtcaatat cgttaactct
    12661 tatctgaatg ggggttgagt gaaagtagct atgcggtttt atagtcatta acaatatatt
    12721 ttttttgacg atcgatatcc tcgtaaatcg agttgcggct aatgaactcc ggaacaatat
    12781 ccttcatcag tgaaacaacc ttgaagttgt tgtgttcggg tagagtttca attagcctgt
    12841 ttattttcgg aatgacattt tcggggatca gatcaggaac tttggcaatc atgattttgg
    12901 ggtgataggt gggtatggtg ttttcagtgt tgtttagaag ttcctcataa agtttttcgc
    12961 cagggcgaag ccccgtgaag ttaatttgta tatccttgcc cagggtgagt cccgagagct
    13021 taatcatttt ttttgccaga tcaagaatct taaccggttc gcccatgtcg aatataaaaa
    13081 tttcgcctcc attacccatg gctccggctt caagcacaag ctggcaagct tccgggatgg
    13141 tcataaagaa tcgcgtaact tcgggatggg taatagtaac cggaccaccc ttctcaattt
    13201 gttttctgaa acggggtata accgagccgt ttgaaccaag cacgttgcca aaacgcgtgg
    13261 taataaagtt ggtatcaacc tggcagctga gcgattgggt aaagatttca gctatgcgct
    13321 tgcttgcacc cataacgttg gtggggttaa ccgccttgtc tgtagaaatc atcacgaact
    13381 tctctactcc ataccttacg gcggtttcgg caattagctt tgtgcctagc acgttgttga
    13441 aaacagcctc agtagggtta ttctccatca tggggacatg cttgtaggct gctgcatgga
    13501 aaaccacgtt gggtttaaaa acatcaaaga gtttatttac cctatagtca ttggtaacgc
    13561 ttccaattac agattcaaag tcgtagaagt gcaggttttc ctttaactcc agttcaagat
    13621 cgtaaagcgg agtttcggcc tggtcgaaga gcactatttt tttagggctg aatttggaga
    13681 tctgcctaac catttcgctc cctatcgatc ccgctgcacc cgttaccagc acaaccttgt
    13741 tctttatgta gttttgaatt ctcctcttgt caagaacaat aggagggcgc tctaaaaggt
    13801 cttcaatgtt tatgcttttt agctggctgg tgttaagctc tccgttaatc catgcggtag
    13861 catcgggtat aacctgaacc tttacgttga gctgcaggca cttgtctatt attttgttct
    13921 tttttacagg gtcaagattt ttttgggcga ttaaaatcct tgaaacgcca tacttttgaa
    13981 caaggaattc aatttttgag gtgttgtata tcctgacatc ctcaatggag tttccctcct
    14041 gcttgcagtt tgggtcaata aaggccacca cctttagacc agccatgttg tccttgtcaa
    14101 ttgccctttt tgcagaagcg gcaaattgtt caatgcctac aatcacaata ttttcggctt
    14161 caatatcaac atttttaatg gtataataaa gcgttttgta gaatagcctg gagccaatca
    14221 taagaatatt ggtgccgcaa aaatctatga gcaggatgga gtttggaatt aggaactggc
    14281 ccgatttaga gtaaactata aagttgatgg taaacagtac tattgtgctg gaagtggtta
    14341 ccatgaatat cctttcagca tctttcaaac tggtgtaccg aattaagcct gcgtaggtat
    14401 gaaacgaaat aaatgcaagc gcccttacaa acaggactag cggagcaact agctttaggg
    14461 cattaacttg ctcgcttcct atccgaaact caaaccgcac gtagtacgat agagtaatgg
    14521 aaaccaggca gatgcataaa tcaataacga aaaccaacca gcggggtgca tgttttacgg
    14581 gtggaatcat gttaggtaac attttatatt caaatttagg agaaaaattg tatcgttaga
    14641 aaattttttt aagcaaaaat tattcctaaa atgagaatga tgaataaatt tcaaaccttt
    14701 ctggtttatt ttaggtagta gtaatgcggg aagaaagtcc tggagagcgg gttagttgta
    14761 tattcccacc aaatcacccc tgaatgatac ttggtatgat ttcagcttat accccttagt
    14821 tgggtagcca ttattgtaga agtagtaaac cgtatggcaa tgagggcagg tggcagttcc
    14881 tgagccgttt tcatcaaggg ccaccgatgc atttactgca atgtgctgcg ggcaggtagc
    14941 atcaaaggca actatttcct cctggtttag acggaatact ataatcccgt tgttgttgta
    15001 accctcatta ggaattttta ccgcgcccat gggaacggtt atgttgttgt aaaagggttc
    15061 gttaaggtag aattctatgt gaactgggac atcgggaaca atttcgggct cgtcctctcc
    15121 acagctgcta ggcaaaaaaa aggatgctgc tattactaaa agtttagcta tctttgctct
    15181 catggtttta ttccggtaaa tacattacaa aaatacaatt gtttttgtta cttataatac
    15241 cggcttaaac ctgatggtga aaaacagcaa cggattgccg ttcgtaacaa tttaacggaa
    15301 atgacgttag tgtgggtagt tgaatttttg ttaactaaat atttgctgaa atggatggtg
    15361 gagatatcct attcataatc attgttacca ttttaggaat tatcagctcg gtttccagga
    15421 agaaaaaaga ggcggccaaa cgggccatga agcagaccga agcagaggag cctaaaaact
    15481 attcagaagg ccaaccagtt gaaatacctg aaaataataa tgaagacata tatacatggc
    15541 ctcagcaatc agtaagcgag tttccgtcca acgacactat gcaggatttt acttctaatt
    15601 caaatccgcg aaatgaaggg aagtctgtgt tgtctggctg ggacaatatg gagggtgagg
    15661 ttaaatctgt ggagtatgag atgccgtcag tgcaggtaag cgaagaggag ttgtacgccc
    15721 ctaaaaagta tgaagagctc gataaaattc ccacttcttc gggaagcagc ctactcgacc
    15781 agcctgttag tccctacgag acaacctacg agggtctagc ttctagctat cagcctattg
    15841 ctttcgagga gcaagaggaa gagtctaaag cccccgatat gatttttgag tatgaaaaag
    15901 tgcttgagca ggaatcgaac caagctggca ttttggatga ttttgatccc gccaaggcag
    15961 taatctactc tgaggtaatt aataaaaaat atttttagct ttcatttcat tccgagattt
    16021 ttattctaaa atttgttata tttgcaaaag atgagagttc cgagcaatcg gaattctttt
    16081 ttatttcaga tgtataagga ggttgaaatg tccaaagtga actacttaac cgaagaaggc
    16141 cttgagaagc tgcgtaagga gctcgaccac ctaaagagtg tggagaggcc agccatttcc
    16201 aaaatgattg ccgaggcaag ggacaagggc gatttgtcgg aaaatgccga gtatgatgca
    16261 gccaaggagg cccagggcat gctggagctt cgtatttcaa agcttgaaga cctagttgcc
    16321 aatgccaggg ttattgatga gtcgaagatt gataccgaac gcgtgcaaat actcaaccgc
    16381 gttaagctta aaaatctgaa aacaaatgct gtagttgagt acatgctggt atcggagtcg
    16441 gaggctaact tgcgcgagaa caagatctct atcgccacgc caatagctaa agcgctgatt
    16501 ggtaaaaaaa taggcgacat agtagaggtt caggtccctt cgggtctgat gaagtttgaa
    16561 attcttagca tatcacttta atcctgtcgg gttatggctt ccattttctc aaaaattgtg
    16621 aatggcgaaa ttccctctta cagggttgct gaagacgaaa aatattttgc ctttcttgac
    16681 attaaccctt tggctgaagg gcacacctta gtaataccca aacaggaggt tgattacctt
    16741 ttcaacctcg atcaggaaac cctatcggga ttagtccttt ttgcccagcg ggttgccaag
    16801 gcaattgaaa gcgtgattcc atgtaagcgt attggtgtag cggttttagg gcttgaggtg
    16861 cctcatgcgc atattcacct cataccaatt aattccgagt cggacattga tttccataaa
    16921 ccaaagctaa agctgaatcc tgaacagctt gatgcaattg cccaaaaaat tgccacgaaa
    16981 tttgaatagc tgtgtgtggt agcgggcttc cattccgtgc ctctttgcag gacaatcagg
    17041 ctaatacttc aacctatcct agaaagctta cgaaaagcat taggtttaaa agggttacta
    17101 cggctccaag accgtaaagt gtccattttg taaagggact gtttttgtac tttcccatca
    17161 ccttttcgga ggaggtgagc ctaacctggg tgaatatcgt gaagggtagt tgaatgctta
    17221 gcaccatttg ggatattagc agtgctttaa aagggttatc tatggcaaat ataaccagca
    17281 cagcagctac cagcgaaagc agaattccaa gttttgaatg gttgtccttt acgtcgtaag
    17341 gctcgttaaa aattcctgcg aatattgatc cggcagccat ccccgaggtt atggtagagg
    17401 atatccctgc aagtagcaga gctactgcaa atatatcggc tgcactgctt ccaagtaatg
    17461 gtattaacat gttttgagcc tgacttagct ccgaaaccgg cgtttgggtt ttatgaaaag
    17521 tggttgcagc aagtagtatc atggagctgt ttatggccca tccaaccagc atgctgaaaa
    17581 gggtatcgaa aaattcatag tcgagttgtc ttcgtatcac cctgtcgtcc tgaaggttcc
    17641 actgtcggct ttgaattacc tcggagtgca gaaacaggtt gtggggcata actactgccc
    17701 cgagaacgct catgataacg agcatggagc cttcaggaaa cgatggcgtt acggaggatt
    17761 tgatggcttc gggccagtca atattaacca ggctcaactc gtagataaac gatagtccta
    17821 ttatggagac aaatgctata atccactttt caattgcccg gtagctattg gtgaatagca
    17881 tcactagcac aaacgccaaa acaagcactg cgccaatctt gataggtatg ccgaatagca
    17941 tttgtagtgc tattgcgcct cccattatct cggccagcga ggttgagact gatgccagca
    18001 ttccggtgcc caggataaac cttgaaacgc caggttttag gtactttgtg gttgcttcgg
    18061 acaggcacaa cccggtggca attcccaaat gtgccacgtt atgctgaagg acgatgagca
    18121 taatggtgct caggctcacc atccatagca gcttatagcc atagtcggct cccgccgcga
    18181 ggttggaagc ccagttaccg ggatcaataa atcccacagt taccagcagt ccgggtccaa
    18241 taaacttgaa tatttccatc gctcccatca cgggacgatg gtttttggaa tcaaaccttg
    18301 taaaaatctt tagcatcttt aaaccgctat ttatccgtta cctgttttgt tgcttaccac
    18361 cttaatatta gtaacccttt tttaggtgaa aaagttctac tttttacctt tgcgctaact
    18421 caatcttttt ctgctgttgc gcaatatgat atactttggc ttggtgctcc aatgctgact
    18481 tttccgttta ttcatagtgg agtagagcta ggcctcatct gtcatattgc ttttcataac
    18541 catgtccacc gtttttacca catgctccag ttcaaaaccc ctcgacaacc caaatcggac
    18601 aagttttgcc cgtaagtccg acggactttt agccttaacc tggttggcct ttgtggtaat
    18661 aatattttcc agagttgccc ggtcgaattg gggctgaagc tcgagcagcg cctccttaat
    18721 tatagattcg ttaatttttt ttacccgcag agctgctgca attttaatgc tgccccactt
    18781 gtttagctgg ctcttttccc ttacaaacat ttgtgcatac cttctgtcat caacaaatcc
    18841 ctcatcaacc aacatggaga caatcttttg agcctcttcg ttattaatcc cccatagttt
    18901 aagtttctgc atgatatccc acacgcactt ttcctgcctt gcgcataggt tttggagccg
    18961 tgttaaagct ttatcaatac tcactttttc tctgctatcc ataattacag tgggttggtt
    19021 atgtttagcc ggtataggtg cccgaaatca tccggtagcg ttcatttata tcctttcgca
    19081 acgtaacctt gccaaaatgg tttgcctcca gcagctctac catttggttg ccaaactcct
    19141 cattaatttc gaggaatagc cttccccctt gcttaatcaa cttggaggac ttgcttacaa
    19201 tagccttgta aaattgaagg gggttatcat cacttacgaa cagggctagc gggggttcgt
    19261 ggtcgagcac atttttgtgc ataaacctcc tttcggagct gcgcacgtag ggtgggttgc
    19321 tcaccacaat gtcgaaggga gcgtttggaa tcaggctgcc ggaaaggatg tcagcctcta
    19381 caaagcaaat cgaggcgctg tatattttag cgttatgcct tgcaacttta agcgcgtccg
    19441 gggaaatatc aacagctgta agggtagaat cggggatgtt ttgtgccagg gcaatggcaa
    19501 tgcaacccga gccggttcca atatccagaa ttttaggttt tggtaagtgg caatcagcaa
    19561 tcacccatcg aacaagttct tcggtttccg ggcggggaat gagcaccgat gggttaacag
    19621 ttagatttaa ctccataaaa gtggtttcgc ccagcaagta ttgaattgga acgcctcgaa
    19681 ccagctggcc tgtcatgtct aatatatttt tggacaggct ggggctgact tgggcgtttg
    19741 gggttaggtg aacatggtag tagggaattt taagcacatg ttccagcagc atcctacgca
    19801 aggagtttac ctcaccggaa gtaaaaaatt ggttcaggac taagcccacc tcatcaaata
    19861 gctgcctttg agtgtaactc ttcatttaaa aatttaactt tgtcatagca caaatttagg
    19921 taatatttat ggacaacctc gatgtggata agctttacat gtctcgctgc ctggagttgg
    19981 ccttaaacgg gatgggaagt gttgctccaa accccatggt gggtgctgtt gttgtacaca
    20041 acggtaaaat catcggtgag ggatttcatg cgcagtatgg tgaggcacat gccgaagtta
    20101 atgccattaa ctcggttagg gataaatcgc tgttgcagga ttcaacgctt tacgtttcgc
    20161 ttgagccctg ctcgcattat ggtaaaactc ctccatgtac cgaccgaatt gttgagtatg
    20221 gcattccgcg ggtagtggtg gccacaaccg atccaaaccc cagggtctcg ggccgcgggt
    20281 tggagcagct gcgtaagcat ggagttgagg tttctctggg tattcttgag gatgaagcat
    20341 tgctgctaaa caggaggttt atcacctttc atacctgcaa aagaccctat attattctta
    20401 aatgggctca aactatagat ggatttattg atctggttag aactgctgaa acacctgttg
    20461 gttctgtttg gataactaac gagctggcac gctcgctggt acatagatgg agaagcgaag
    20521 agcaggcagt gatcgtcgga accaacaccg ttgaaaggga taacccacgc ctgaacatcc
    20581 gcagctggag tggaagaaat cccattaggg tgattgttga tcgaaagttg cgcctgagta
    20641 gggatgccaa tgttttcgat ggaagtcagc ctacaatttt gttcattggg aataacagca
    20701 gcgcgctggt gcgtaaatcg gaatttgcag gcatctcaaa cctggagatg gtaacaatcg
    20761 actttgccaa aggctccgag cagcaaatcc taaaagagct gagcgatcgg gatgttattt
    20821 cattgataat tgaaggcggt gcaacgctgc tcaacaactt cattcagaaa aacttatggg
    20881 acgaggcacg ggtatttgtt ggcaaccgct tttttggcga gggagtgaag gcccctcttt
    20941 ttaagggcga tcctgtatcc tacgaagagg taggggacag caagctattc atttatcgga
    21001 atcagcaggg aagaagcaac aggtgaaata ctaataacct tcagcatatc gtttcaaatt
    21061 taccattgta gttttgcagt gccaggcgta accgcgttgc aaaagttaca gtgctgcaaa
    21121 tttaatagca tccgaatccc atgttggcgc tttttctatg gccggaataa cctcctgggg
    21181 ggtttccaca aatgtccaaa tacctgcatg ttcaggccgc atgaagtgct catcaatcat
    21241 cttggccaaa agtaatttta ggtaatcgta gaatccatcg gtattgagaa tgattatggg
    21301 tttcgtaaat tttcccaacc tttttagggt gattacctcc atcagctcct caagcgttcc
    21361 acagccgcca ggcagggcga ctacggcatc aacatcctta atgaggagct ttttccgctc
    21421 atgcatatcc ttaaccagct ttagctcggt gagattctta tgtccccact caactttatt
    21481 cataaatcgg ggcaatatac ctaccaccat gcctccattc ataagagcgg tatcggccag
    21541 acatcccata agcccgtgag aaccaccacc atacactatg atgtcgcctc tgttggccaa
    21601 ttccgaggct agctgacggg ttgcatcaaa atacttttgg ttaatttttg agctggacgc
    21661 acagtaaacc gaaattctca taaggaaaag attaaatggg ctctacgttt gcaagaacat
    21721 gcggttagtc ctttatttcg ggaatgccgc tgtttgtaat gcacagctga tggagaatac
    21781 cggttagaat ctattgaaaa ctccatttgc tgcaacgcga atggtggaag ggggattttg
    21841 ggtaaagata ataatgaaga tgtttagtag aaataggtaa aaccaccaca acatcgggtt
    21901 aataacccta aacatcatgg tggtatgaaa atttttaaaa aaactacctg agtagggctt
    21961 caagtttgga aaccaggttg cttttaggaa cagctccaac ctgcttgtcg gccagctgtc
    22021 catctttaaa gaacagcacg gtagggatat tcctgatgcc gaaacgcgca gcaatgccag
    22081 ggttattatc cacatcaacc tttgcaacaa cagctctatc cttgtattca tcggcaattt
    22141 gttcgatgta gggggcaatc attcggcagg ggccacacca ttctgcccaa aaatcaacta
    22201 cagcaggttt gccggcattc agcaccacct cgtcaaagtt ggaatcgttt acttcttgtg
    22261 ccatgacaac tattgtttta aattgataat ctaattcgtt taccagtaca aaattaaaac
    22321 gaaaattaat accaaaggtt cagcttgtaa ttagtttata ctccagctca ttatctgtca
    22381 ggaagtgaat aagttcggtg ttgatgctaa cccggtgcga tcgggaaaac aggtcaaccg
    22441 aaatcctgtt ttccacatct accaccttaa atcgaagcga taccttggca ttctcgtggg
    22501 ttaacctggt aaaatcgtca ataagtatgt cgttaatttc agtggtggga agggtaatcg
    22561 aaataccctt tatttgtttt tcccgaacgt cgtaaagcat gctcatactt ttgatgtgaa
    22621 gctccaactc ctctggttta aaggctttgg gctgaacttt tcccttaatc atcaccatgt
    22681 aaccggtgta gcagaaattt cgataggttt caaagtcctt tccaaacagc atgatgtcca
    22741 tggtgtcgga gtagtcctca atggtgagcc tggcgtaagg tttgccattt ttggtagtat
    22801 caatcttagc cttaaccacc ataccggcaa tggttacttc cctgttgttg aaatcttcga
    22861 ggtttttaag ttcggccagg ttggtgttgc ataggttctc aatttcaaac cgaaactcgt
    22921 caagtggatg agcagagagg taaatgccaa tgatttcctt ttccttgttc agcttttcga
    22981 tggttggcca atcgtcggtt ttgggaggtt cgggttgtgg tacctcaaat ccgttggttg
    23041 caccaaaaag ggtcatctgc ttactgtttt tttccaattg aacctttgct ccgtagcgca
    23101 tcagggtttc catgaaggtt acacccttgg agtcgggtgt aaaataggcg ccccggctaa
    23161 tggggctaat gttgtcgaaa gcgccagcca gcacgagcga ttccaaattt ttcttgttaa
    23221 ctgtttggag gttaacccgc tccatgaaat cgtatatatt tttaaattta ccctcatggt
    23281 tgcgagtttc gataatgtta gctgctgcat tttcgcccat tcccttgata gctcccaagc
    23341 cgaatctgat gtcgccgttg ggcatcactg agaacttgta gttgctctca ttcacgtccg
    23401 gtcccagcac ttgcaggccc attcgacggc actcgtccat gaaagtggtt atttttttaa
    23461 tatccgaaag attccggctt agcacagccg ccatgaactg acccgggtaa tgtgccttga
    23521 tgtatgcggt ttggtaggcc agaaaggcat agcaggtgga atgtgacttg ttgaaggcat
    23581 attcggcaaa ggcttcccag ttggtccaga ttttttccac tttagcctgg tcgtgtccgt
    23641 tgttgatgca tccatggata aattttccct tcattttgtc catgacgtcc cggagttttt
    23701 tccccattgc ttttcgcaga gaatcagcct caccccttgt aaatccggca agtttctgcg
    23761 agagtagcat cacctgctcc tggtaaacgg ttattccata ggtatccttt aggaattcct
    23821 ccatttcggg tatgtcgtac tctattttct cccgaccatg cttccggttg atgaagctgg
    23881 ggatatactc cataggcccc gggcggtaga gcgcattcat ggcaatgagg tcctcgaatc
    23941 ggtttggctc caaggcttta aggtattttt tcattccggc cgactcaaac tggaaaatgg
    24001 cggtggtatc gccccgagag aagagttcga gtgttgtcga atcatccaag gggatattgt
    24061 ctatatcgag cgattttccg gttgattcca tgataagctg cagggcatcc ttaacaatgg
    24121 aaagggtttt cagtccaaga aagtccattt tcagaaggcc aatgtcctcc atatggttgc
    24181 cctcatactg ggtaacgaag agtttggcct ccttgttgat acaaaggggg atgtagtttt
    24241 ccaaatcgtc gcgtccaatg atgatgccgc aggcatggac acctgtttgt ctgatggttc
    24301 cttcaagaac ctgtgcgtgc tttagtgttg ccctcaccag ctcgttgtcc gatttcagct
    24361 ccttttccag ctcgggaacc tctttaatgg cagccggcag ggtaatgcca atgcgttcgg
    24421 gaacaagttt cgacaaacgc tcggaggttg agaggtcaag gtttaacact cgggcaacat
    24481 cctttatgga cgatttggct gccatggtgc caaaggttac aatgtgggcc acacgttttt
    24541 cgccgtactt ttgaaccacg tactggagca catcctcccg gccatcctcg tcaaaatcaa
    24601 tatcaatatc gggcatggag atgcggtcgg ggttgagaaa ccgctcgaac agcaggttgt
    24661 attttatggg gtctatgtcg gtaattttta ggcagtaggc gaccacggag ccagcggccg
    24721 agcctcgtcc gggaccaacc gataccccca tttcgcgggc agcccttata aagtcccaaa
    24781 caatcaggaa gtagctgggg tagcccatac gttcaattac ctgaagttca aactctattc
    24841 tactgccgat ctcgtcgcta agggtatcgc cccagcgcaa ttttgctcct tcgtaggtaa
    24901 ggtggcgcag gtaatcattg tcatcggtga aaccatcggg aagcgggaag ttaggcataa
    24961 tgggcttgtg gctaagatcg taaaattcaa ccttatccag tatttccatg gtgttgtgaa
    25021 ctgcttcggg attctcggaa aagatggaaa gcatttcctc ctcgcttttc aggtactcct
    25081 gaaaggtgta gcgcatacgg ttggtgtcgt caatccgctt gtcggtgtta aggcatatta
    25141 gcaggtcgtg cgaggcggca tcatcggaca tgacgaagtg gacgtcgttg gtggtaatgc
    25201 actttactcc tgtttttacc gacagctcta acaatgcctt gtttaccttc agctggtttt
    25261 cgtaaacctc ggagtcaatg cggggttcac cggatttgtg gagcatgagc tccaggtaaa
    25321 agtcgtcgcc aaaaatatcc ctgtactcca ttattagcct ctcggcttcg gcgatgttgt
    25381 tgttaagtat ggcttgaggt atttcgcctc ccaaacaggc gctggaggca attagcccct
    25441 tgctgtactg tcgcaggagc tccttatcaa tgcggggctt gtagtagaaa ccctcggtgt
    25501 agccatagct aaccagcttc accaggttgt ggtaaccttc caggttcttg gccagaagaa
    25561 taaggtgaaa gttatggcgg tcctccttgt ccgtgcggtc gaaacggctg ttttttgcca
    25621 ggtatgtttc gcagccgact atgggtttaa aaagttgttt ttcggcctcc ctcagctgct
    25681 cgttcagcag gtctagagaa gggctgttat cagccacact acttccggag cttatgcctt
    25741 taatttcagc ttttatgctt tctatttcct taagaacagg gctattactt ttttttacag
    25801 tattcagaaa ctcctttatg ccgaacatgt tgccgtgatc ggttatggca attcctggca
    25861 tgcccagctc ttttgccctg cttactagca tttcaatgct cgatgctcca tccagtatgg
    25921 agtagtggct gtgaacatga agatgtacaa aacttcccat ttacttaatt gtagtcaata
    25981 ctcagattta cgaaatggca ggatgaagcg ttaccgtcat gaaaacgaag gtgattttaa
    26041 attgaaaatc ttgggattca aatgaatgaa cttagccatt aaccctacct gttatcgtgc
    26101 gaatttacca aaatatgggg taggtaaaat ggatgttgat aactattgaa ttttcttttc
    26161 gcattcaggt ttggttactt caatttggct gtttgccaca tttttactgt agtttgtaaa
    26221 aggagttatg gaatatatga atcttacttc tatttttata gcttagcata ttaaattttt
    26281 ataggatgcg cggaaaacta ctaatgctat tggtggctag cggagtgata ttatctgtta
    26341 gcgcatacgg gcagaataac ctacctgcct atctaggtgt ctcatacatt ccggagctga
    26401 gatggaaggt gtacttcatc aaccataaat ctatcggcga acctctttcc ctgaattttg
    26461 acatgaccag catgtcgtcc tacgagggca attttggtat tcgtaggcta ggggtcagga
    26521 tgggggtgtc ggcccagacg gagaacaacc taataggtaa gttttaccgc tggggcgggt
    26581 acttgggatt taagaacatg ctactacggc tacagagttc caagatgtcg ggcgacctgg
    26641 agtggtcggg agcgctgcct cctggttact atgccaatag ggaattttcc aacaagtact
    26701 tcaacataga tcttttaaag actgctaaag ggggttcggc gtttaacgaa gttggagggt
    26761 actggggtgt tggctatacc tcaatgggtt atccggtaga gattagcacc ctggttaccg
    26821 aaggcggaag ggagaaccag aagtttggaa tgcctgctta tgatacgctc tactccatta
    26881 aatcgtataa tatttgtttc ggatttgact tgcttcgaca gctttgcctt acaggaggac
    26941 ttgaaggttt tgctccggga agacgcgcca tgcgttttgc catgtatgcc tccacgcagg
    27001 ataaggttgg ttttgggccg gccaaaatat ccgattacgg agtttctatg gccgaagcgc
    27061 ttaacccggg tagaactgca gttaccagta agtttttcaa tgtaatggtt cattactccc
    27121 tctccttggg gttgcgctac tacattcatc ccgggccaac ggtagtaatt tttgctctgg
    27181 gatacgactt tgaaggcgcc atgaatgcgc cctttaaagg agcagccgat aacgccgagg
    27241 atttaggttt cgactttttg aactactacc tgaaccatgg tgtttcgttt aagatattcc
    27301 tggcatggaa cagggactgg cagcagaaaa atcagtaact acctgtttac ctcacctcaa
    27361 gcaccttgcc agtgaattct acctttcctg ctgtaagctt aacgatgtaa gttgcagcag
    27421 gcattcctac ttctgctgca gaaaagtagt gggttttttg tcccgcatcc tcaataccgt
    27481 tcaccaaaac gctaacaagc ttgcccgtaa tgtcatacac cgcaaggttt actttgcagc
    27541 tttcggggag ggtgtaggta atggcaacct tgcccttata gggatttgga aatgttgtaa
    27601 acctcagcag gttatccgac accttgtcca catcgcttgg ctgacttaca acattgccgt
    27661 tgaaggctgc cttggtttgt gttgaaggtt tcccgtttgc cggagtgtag gtgcttttta
    27721 ttagcgatag taccggaaaa cggtggttgg ccacatacca gcgataggta acatcctcaa
    27781 tgttaatcag gctgttgttg atggtttgcg tggtgagctt cacctcctta acccgtaaaa
    27841 ctccacttag ctctcttcca ccgggtaaaa tcagcttacc gtagccatcg ccctccaccc
    27901 ggtaggttcc tgaaatggtg ccaacaggca cttcgttaac aaggtaatca cccttaaaat
    27961 ctccggaaaa gctgctgtta aaaccaaacg ggtagcgcat tttgacaaat ggcttgctgt
    28021 atgttatttc ggtgcttcct gtagatgact gaaaaccaac ttgctccagt gagttggctg
    28081 tcatattgaa gataaaccta ttgccgaact cttcaagcac cacgttgcca ccctggtttg
    28141 aggggtaatc cttgagggtg taaacctctt ttaggctgcc ctggaagttc tccgaagttt
    28201 ccagcatgct gaagttccac accacgtttt taccagctgg gccgggttct acgtagcttg
    28261 taagcagcat tgggttattg tcattaggaa tgaatccgtg gttttggctg gtaataatag
    28321 tttgcccgaa taaacttagg gtaagaacca gctgagggct gagcagtaat aatttcttca
    28381 tgacggcctc cttttaggtt tagctatgcc caaatatagc tcaacttcga aatgaaatca
    28441 atacgttgtg cggttaattt tttgttaact tctttaaggg cagctccata agcaaagagg
    28501 ttgggtttag tgagttgaaa agttaacaga atgcttttct caggctggta gaagagtctt
    28561 ggatgtgaag caatgtggcg tttggtgatg gtatggcaaa aaaagttgta ctttggtgtg
    28621 ggtggtcagc agcaacggca aacagctata tctgcccctg ttacctttgg agcatgccgc
    28681 ttacattgtg atttacaggt aaaatgaggt tgtgggcggg tagttcgggc caattagttt
    28741 cccatttcgg aaaggaactt aatccgcata agcctgattt cctcctcggt gtaatcctca
    28801 tcgccaaggg caacaagagc ttcctcgagg ctttcggttt cggcctcatt gcggaagtag
    28861 tcgaatattt cctcctgctt atcctcgtca ataacctggt tgatgtagta gtcaaggttg
    28921 attttggtac ccgagttgac aattgcctca atttcgttca gcagctcatc catttcgaga
    28981 ttttttgcgt tggctatgtc ttcgaggtcc attttccggt cgatgctctg aatgatgtac
    29041 accttgagaa gcgatttggt ggcaaccgat ttaaccacca tgtcctgggg tcggataata
    29101 tccttatcct caacgtatgc cttaatcagc tcaacaaact cctgtccgaa gcgcttggct
    29161 tttccggcgc caactccggt aatactatgc agctcctcaa tggtaatggg gtactggatg
    29221 gccatatcct ccagcgatgg gtcttgaaag attacaaagg ggggaaggtt tttctttttg
    29281 gccattttct ttcgcaggtc tttcagcatt gcaagcagct cgtcatcaac agcgcccgac
    29341 ctgtccatgg gcattccaat agattcatcg tcatcggctt cctcgtactc gtggtccagg
    29401 gtaaagagca cgtcggtggg tttgtcgata aattcctgac ccttaggcgt aataaatagc
    29461 agcccgtagt tttcgatgtc cttgtccagg aacttcatca ccagggcctg acggattatg
    29521 gagttccaga accgggagtc cttttcggcg ccctcgccga aggtatcgag ctggttatgc
    29581 ttgtacgatt taaccgctgc ggtaacgttg ccaagcagga tgttgactat gtggtcagct
    29641 ttaaactttt ggttgactgc cagcaccgtt tccagcacca tttgaagctc ctctttcccc
    29701 tcaaactgtg ttttggggtg taggcagttg tcgcacgagt tgcagttttc ctcatcatac
    29761 tcctccccga agtagttcag aagagttttc cgacggcaca tggatgactc ggcgtacgac
    29821 acggtttcga gcagcagctg cttgccaatt tcctgttccg acaccggttt cccctgcata
    29881 aacttttcaa gtttttgaat gtccttgtag ctgtaaaagg ttatacattg accttcgccc
    29941 ccatcccggc cagcccgacc ggtttcctgg tagtatccct caaggctctt gggaatgtcg
    30001 tagtggataa caaacctaac gtcgggcttg tcgataccca ttccaaaggc tattgttgcc
    30061 acaattacat caacctcctc catcaggaat ttatcctgat tttccgagcg ggtggcggca
    30121 tccatccccg catggtaggg caacgcctta attccgttga ttttgagaat ttcggccatc
    30181 tcctcaacct tcttccggct aaggcagtaa atgattcccg acttgccctg gttggacttt
    30241 atgtacttga tgatttcctt aactgcattc accttggggc gtacctcgta gtacaggttg
    30301 ggcctgttga acgacgattt gaaaacactg gcgttgagca tgccgaggtt tttctggatg
    30361 tcgtgctgaa ccttgggggt tgccgtagcg gtcagggcaa ttataggagc tgtgcctatc
    30421 tcgttaatta tggggcgaat tcttcggtac tccggacgga agtcgtgccc ccattcagaa
    30481 atacagtgcg cctcgtcaat tgcgtagaat gaaattttga tttgctttag aaactgtatg
    30541 ttttcctctt tggtaagcga ttcgggtgct acatacagca ttttggtaac accgttggta
    30601 acatcctgtc ttaccttttg aagttgggtt ttattaagcg aggagttcat aaagtgggca
    30661 atgccctcct ccagaacaaa tgcgcgcatt gagtcaacct ggttcttcat aagggcgatg
    30721 aggggcgata tgatgatggc ggttccatca agtattaggg cgggcagctg gtagcatagc
    30781 gatttgcctc caccggtggg cataagaaca aacgtgtcgt tccctgtaag aacattgtga
    30841 ataattgcct cctggttgcc cttaaaggtg ttgaacccga aatacttgtt aagatactcc
    30901 tttaatgata gttcagatgt gttaacgttc atgatttaag tttccgactc ctaattttaa
    30961 actgtttaac tgttgctttc caccctacat tacgttacgt ggataaaaaa tagatacttt
    31021 tgcttttata aatatacgcc atattttttt tagtggttca aatttaaagt catttaacaa
    31081 aaggatgaca aaagtagcaa atgaaaggga aaaaatgaat aatgatgcca tcagaaaact
    31141 agctgttgaa acaatccgaa ccgaagccga tgccattgaa aaactcgccg ggtttattga
    31201 tgatgatttt gttatggcag taaacctgat ttttaacgga aaaggtaggg taattgttac
    31261 ggggattgga aaaagcgcga ttgtggctga aaaaattgta gccaccttca actcaacggg
    31321 aacgccagcc atattcatgc acgctgccga tgccattcac ggcgatttag ggattattca
    31381 gcccgatgat gttgttgttt gcctctcaaa gagcggcagt actcccgaaa taaaggtttt
    31441 ggtcccacta atcagaaatt tgggcaataa gataattgcg gttgtttcga acaccgattc
    31501 ctacctggcc cgttcatccg attttgtgct taaggcaaca gttgaccggg aggcatgtcc
    31561 taacaacctg gcccccacat ccagcactac agcccaaatg gttatcggcg atgccctggc
    31621 agtttgccta ctcaaacttc gcggctttac cccggccgat tttgctcgct ttcacccggg
    31681 tggagccctt ggtaagaaac tctacatgcg tgtttcagac attttcacct ctaccgatgt
    31741 tccaaaagtt cgccccgacg atcgaattcg atcggttata ctcgaaatat cctcaaagcg
    31801 cttaggcgcc accgcggtgg ttgactccaa cgacagggtt gttggcatta tcaccgacgg
    31861 tgatttacgc cgaatgctcc agaagaatca gagcatcgaa ggccttgccg ccgccgatat
    31921 tatgaccgtt aacccaaaaa caattgacag ggaggagttg gcaattacgg ctttcaacct
    31981 tatggagcag aataaaatca cccagctgct cgttacccat gaggaccgat atgaaggggt
    32041 gatacacatt cacgatattc tcagagaagg tatagtctaa tttccttaag gtgatggctg
    32101 gaaaagcatc gaaacataat gaaatgactt tctggcagca cctgggtgaa ttgaggaata
    32161 tttttatccg ctgcatgttg gcagttgttg tgctttcaat tgtggcattt tgcttaaagg
    32221 acattatttt ccagcaggtt gtacttcgcc ctcgaatgcc cgagtttgtt accaaccggt
    32281 tgttatgccg gttggctgag cagtggaact ctccggttct ttgcatcaac tcaactattt
    32341 taccgcttat caacattgaa cttgctgggc agtttactgt tcatttaagg gtggctttct
    32401 ggagcggggt aattatggct tttccgtata ttatgtatga acttgccaaa tttttcagac
    32461 cggcgcttaa gcagggggaa tcagctatgc ttagaggtgt agcattaatg tttacccttt
    32521 gctttgcaat tggcgctgct tttgggtact tcataatagc acccatatcc attcattttt
    32581 taggaaccta ctcggtgagt gcgcaggtag tcaaccaaat cactctgggt tcatacctgt
    32641 caaccgtttt aggcctcatt tttgcaggtg gcgcagcctt tgagatgccg gtagttgtgc
    32701 tgtaccttag ccgtttagga gtggtgaaac cccaattcct atcccgatat agaaaagttg
    32761 ccattgtagt tatcctggtt atttcggccg tgataacacc tcccgatgtg ttcagcatga
    32821 ccattgttgc catcccgctt ttgcttctct acgaggtgag cgttttgctg gcccgaatgg
    32881 agtttaagga ttgattttta tgattatgag gagattgttg ttttttctag cacttcagct
    32941 ggcatttgtt tcatccattt ttgcccaggg gcagataacc aaaatcaggg gtagggttat
    33001 tgacgctcaa accagggagc ctatagccta cgtgaacatt gcttttaggg gacttcccat
    33061 tggaactatt acctccgaaa aaggtgagtt tttcctcgaa acaagaacta cgggcgactc
    33121 acttaccttt tcttatgttg ggtaccagtc gaaaaccgta gcggttaaaa aggggtattt
    33181 tcaggagatt aacgtggaac ttcagcccga ggctattgag cttgccagca ttgtagtaaa
    33241 accaggcgaa tatcaggcaa accgtattgt gaaaaacatt atcaggaata aggataaaaa
    33301 caacccggcc aacctcagct acttctgcca tagctacaat aaaattcagg ttgacctgaa
    33361 caatgtaaac cttgaagctt cgcaacgtaa gtttttgaat cagtttaaat ttatttggga
    33421 ctacgtggac accaatgcca taaccggaaa accctacctt ccgattttca tcaccgagtc
    33481 gctttccgac tactactacc gttcaagccc aaaaatggag aaggaaatta ttaaggctac
    33541 caaaatttca ggtgttaaca acgagagcgt tgcgcagttt acgggtatga tataccagaa
    33601 cgtgaacatt tacgataact acatcaatat tttcgatcag ggtttggtta gcccgatagc
    33661 caatgccggc ttaatctact acaagtacat tctggttgat agcgcctttg tgggaaatcg
    33721 ctggtgctac cagatttcgt ttaagccccg tcggaaaatg gagcctacat ttaccggcga
    33781 tttttgggtg aacgacacca cctgggctgt ggtgaaagcc cagttccgcc tttccgatat
    33841 ggttaacctt aactttgtaa acgacatggt taccacctcg gagtacgtcc caattaacga
    33901 taccctctgg ttcccgaagc agctaaccat gtttgttgat tttaacctta ccgataagga
    33961 ggccggtttt tttgggcaca aaacagttac ctacagcgat gttaccctta atcccgactt
    34021 cccaagcgag attaccgagc agccaaccag ccttaaggtt atggagggtg cgctaaatca
    34081 ggataaagag tactggaata agacgcgacc ttttgagctg acgcccaagg aggccaacat
    34141 ttacgccatg gtggactcgg tgcagcaggt tcctctatac aaaacttttg ttgacataat
    34201 caacatgatt gtgaactact actacgttgt ggggtacgtt gagattggcc cctactacca
    34261 aacatacagc ttcaacgaaa ttgagggtaa ccgctttaag gtgtcgggac gaacaagtaa
    34321 cgagtttagc actaaagtta tgctatcggg ttttctggcc tatggcgaca aggacaaccg
    34381 attcaagtgg ggtgttggtt ccatgtacat gataggaaag aatccgcgcc aggtgttaag
    34441 ccttgactac aaaagcgaca tcgagcaaat gggacaaagc ccaaatgccc ttaccgagga
    34501 caacatcctc acctcgtttc ttcgccgaaa ccctaactac aagctaacca tggtgaagga
    34561 ttttagcgtg tcgtatgaaa aggagtggtt caccggattg tccaatacaa tttatttcag
    34621 caacaggata atgtacccca cggagtacat cccatttctc ccggttggcg gaggtttgca
    34681 gctgaaaaat atcaccacct cggcagtaac ccttaacacc cgctgggtaa aagatgaaac
    34741 ttttgttcag ggtgaattca cccgggtaag catgggcagt aaatggcctg aggtgaactt
    34801 cgacattacc aagggtataa aaggcgtgct gggtagcgat tacagctact ggaagctgca
    34861 cctgaactac taccacaagt ttaacgttaa tccttttggc tacgcccggg ttattgccga
    34921 tgccggtaaa acatttggag aagtacccta cccgttactt cagcttcacg agggtaacga
    34981 aacctatgct ttcgacaggt atgcattcaa catgatgaac tactacgaat ttgcctccga
    35041 tcagtatgcc agcttgtacc ttgagcacca cttccagggt tttttcctga accgatttcc
    35101 cctgttgcgc aggcttaagc ttcgcgaggt ggccaccgcc aagggactca tcggctccat
    35161 cagcaattcc aacatgaatg tgcttcagtt tccagagggg ctgggctccg tatcaaaacc
    35221 ctacgttgag gttggggtgg gagtggagaa tattctcaag gttattagga ttgatgccat
    35281 ttggcgcctt acccatctgg ataatcctga tatttcgcct tttggaatac gggtggggct
    35341 acaaattatt ttctgagtag caacaatttg tttacttttg gagcttaaat aatgcaagct
    35401 agctatgcgt ttatttactg aaaacctggt aaaacggtat aaaacccgaa ctgttgttaa
    35461 gggcgtaagc gttgaggtga gccagggtga aattgtcggg ctgcttgggc ccaatggcgc
    35521 cggaaaaaca acaaccttct acatgattgt tgggctggtt aaacccaacg aggggaaaat
    35581 ttttgttgat gatgttgaaa tcaccggcga accggtttac aagcgcgccc agcgcggtat
    35641 aagctacctg gcccaggagg cttcggtttt caggcacctt agcgtggagg ataacctaaa
    35701 agctgttctg gagatgacca agttcagcaa ggagtaccag atggaaaagg tggagtcgct
    35761 tctcactgaa tttgggctgc agaaaataag aaagagtaag ggaattcaac tttccggcgg
    35821 tgaacggaga agaaccgaaa ttgcaagggc actggcgctc agtcctaagt ttattcttct
    35881 cgacgagcca tttgccgggg ttgaccctat tgccgtagag gatattcaga atattgttgc
    35941 ccaccttaag gataagaaca tcggtattct tattaccgat cacaacgttc atgaaacgct
    36001 ttcaattacc gacagagcat atttgctata cgacggggaa atacttaaat cgggaacatc
    36061 cgaggagctg gccgacgacg aacaagttcg aaagctctac cttggccaga acttcgagct
    36121 tcgtaaataa tttttccttt taaggctact cccggttggc tgcttaaaaa aatcagctaa
    36181 ctttgccaaa aattatgcgg atatggcgta attggtagcc gcgctagact taggatctag
    36241 tgccgtaagg cgtgtgggtt cgagtccctc tatccgcact tgacccggaa aagttccggg
    36301 ttttttgttt tgctaccagc cttaatagtt cctctcccct caaatccgct atttgtagtc
    36361 ataatagccc ttaactccat tgccattgtg gggggattgg tggtgaaggt gtaagcaatt
    36421 cattttcccg atttactggc attcaaaatt tttacctact attgtacgat taaagttttg
    36481 ctactactta cagctaaatc tgccatcaca tgagcaaggt tgcgcttttc ccgggttcgt
    36541 tcgaccctat tacagtggga catgaatcgc ttgtgctgcg cgcactatcc attttcgaca
    36601 ggatagttgt tgccattggc aacaattcca gcaaaaaatc gtacttcgat attgatttac
    36661 gcaaggccat gattgaaaag gtttttgcaa acgaacccag ggttgaaatt atttgctatg
    36721 acggtttaac ggttgatatc tgcaacaagc tgaatataaa ccacatcctt cgtgggctta
    36781 gaactgcagc tgattttgaa tttgaacggg ccatagccca ggtaaacagg gccatgtatc
    36841 cccatattga tactattttc ctacttaccg ctcctgagca tacttttgta aattcctcag
    36901 ttatcaggga tatacttcag caccacggcg atgccactaa gtttattcct gcctccatca
    36961 acatatacga ctatatgggc taaaaccctg caaaaaccca agggtggttt acctgcttag
    37021 gcaggatatt tgtttactcc accggttaaa gcaatattac ctaacattct agcaaggctg
    37081 aaaatgagaa atttggtgct ttccctgttg gtgtttatta ctcttggagt tacgggagta
    37141 ttggctcaac aagccttagt ttacggcact gcggccgatt atgccggaac tgatctggtt
    37201 ttttacacct actccgacta catcacgctt acagaagttg aaattggcag atgcagggta
    37261 aaggcaaatg gtaactttga ggggagcgtt aaggttttgg aaacaaccta cgtattcagc
    37321 catttaggga tttaccgtat ttactttttt gccgaacctg gtaagcgtta ccagctggtg
    37381 cttcctgcaa gggaggagaa gacagaagct caaaggttat cgccattttt tagggaaacc
    37441 gacttgcagg ttggagttgc taacatcagc caatccgata ttaactttct ggttaatgcc
    37501 tttgatattc gctttaatgc ccattttgac caaattgtag cggataccta ccggggtaag
    37561 tcccagaatg tagattcgct tattaacgtg atggaggaaa agtttaagaa tgagccaaat
    37621 tcctacttca aggcttaccg cgagtaccgg tatggtctgc ttcggcaggt taccctcagc
    37681 cggaaatcgt ctggaatttc taacgagctg tttcttaaca agccaatcct ttactccaac
    37741 ccctcgtata tggagctatt caacctggtt tacgataagt acttcatgtt cttctcacgt
    37801 accagtaccg ggaatgccat tttcagcacc atatcgcagg agaaaagcct aacccagcta
    37861 aaaaaactcc tcgccaccaa tacagtgctc agcaacgaca ccctaatgga gctggttatc
    37921 ctcaagtcgc tctacgatga gttttttagc agtaactttt cgcgctcggc tctgctaacc
    37981 cttctcgact cggtttatcg cacaactcaa atccccgaac atgccataat tgccgaaaat
    38041 atccgaagcc gaattaccaa gttgctcccc ggatttgtgc caagtccttt caagctgaag
    38101 gatgtaaacg gccatgaagt tacgctcgac cagcttgagg gaaaatacgt ttatctgaac
    38161 ttctgcacca ctaccagcta cacatgcctt caggattttt ccttgctgga aaaaatctac
    38221 cggaagtata gaaaaaaatt agaaattgtt accatttcgg cggatatgga gaaaaaggat
    38281 ttggaaagtt ttcttaagac taccgggtac agctggacat tcctgcactt tggagaaaaa
    38341 cccgacgtga ttaaggattt tgatgtgagg gcttacccca cctacttcct gattggcccc
    38401 gatagaaaaa ttatactttc tcccgctcct tcgccaaggg aagggttcga gcagcgattt
    38461 tttgagctgc tccggagcag gggcgaaatc tagcttcgta aactcgcaaa taccatccgg
    38521 atagatgggt aggagtcttt caaacggttt tcaaccccat ctgcatcaac ccattcagct
    38581 atttcaatat cttcctcaac ctgaggcttc aggctgagag tgcccctaac ctgcatcaaa
    38641 aaccagtggg tttgcttgag catagtgttt cccttctcct cgtaggtgtg gtaggtgcat
    38701 gtaagcgggc ggacaaccgt tagacctgaa attcctgttt cctcctccac ttcgcgcaag
    38761 cctgcctcaa caggggtttc tgaaggttcg accttcccct ttggtagatc ccattttccg
    38821 cgtcggtata tcatcagata ctgtccagcg ttattccgca ccaaccctcc ggcagctgca
    38881 aggaacttaa actgagaggt aaaccaggag aagagcttat ccacgctgcc gttaccaaca
    38941 aaaacattac aaattgccgg gtgactttga aaaattttga ctaactttgg aatgtcgtag
    39001 ctggaggcat gtcccaaaaa ataccctttt tcgttgccaa cgaagtaacc agccaaatcc
    39061 gatgtgatgg taaacctcct atcgttgaaa aatatgacgt actctgaagc cataaatgct
    39121 gaatcaatcg ctgcaaattt actaaaaatt aaggcaattc gtttaagtcc gacagctcct
    39181 tttacctggg cttcgggtct taaatcgcca atatactgcg ataaccgaat aaccctttcc
    39241 tatccggatg ttcgaaaccg gatatgcaac gcctttgtgg agcttgttcg tcggttgtat
    39301 cccgacgtcg agctggttgc gggagtggct accggtgcaa tagcccacgg aatgcttgtt
    39361 gccgacaggc ttaacctgcc gttcgtttat gttagatcgt cacccaaatc gcatgggcta
    39421 gccaacagga ttgaagggga ataccgtcgt gagcaaaaag tggtggtaat tgaagacctc
    39481 atttcaaccg gtcagagcag cattgcggcg gttgaagccc taaaggaagc tggctgtaat
    39541 gtgctggggc ttttggctat attcagctac cagcttgaac aagctgatga aaattttacc
    39601 agcgccggtg ttgagcttac cactcttacg aactatagcg aacttatagg ggtggctgtc
    39661 agtacaggcg ttgtgaatgc cagccagctg cagctcctga tgtcgtggca cgaaaaccct
    39721 gaatcatggg gaaatataac cttgtaggct gaatttctct ttggcgggca taaatatatt
    39781 tcgttcattt tattacattt atcctaattt tcaactaaac gggtgctagc atgcccatca
    39841 ttggcacgaa atacagactc ttcatatttg ttgttgagag caaccccgat tgtgttgcca
    39901 ggttgatgca aggcctggaa gttcctgaac aatccctaat aaaggcattc tcgagcggag
    39961 agagttttat tgattttttc cccaagtcca aaatccccaa aagaaagcta attctgattt
    40021 ttttgggcta taccttttcc gatgaggcca accaaaacat gatgaacggt atcgaaattc
    40081 tagaaaccgt taaaatgctt aaccctgctg ttcatgttgt aatgctaacc cacaacggcg
    40141 agcaggagta cggtggtcat gcaaaaagca tgggggccaa cagctgcatt atgaaggacg
    40201 aaagcatggt aatcagagcc aacagcctta tcaggttact tctaagcgaa aagaaattgg
    40261 gcaataggcg acgtcagctt aaagttgcag cggttagctg gttagttgcg ctggtttgct
    40321 ttttactctc cgttggtgtt tgctgctttt cctagccaac ctgccaaaat tgcgcctacc
    40381 gtgtccgctt ggcagttgta ttaagaatca cccgacaaag tgtaattatc aataaatcaa
    40441 catgatgata atcattttct atgccctacc cggtggtaaa ttttgtatgg cattcaactt
    40501 gcaggtagag gctggaaaaa atttgttaaa cctttaaatg tggatgcaat gcaaacgggt
    40561 aaaattgggg taacaacgga gaatattttc ccgatcatca aaaaatttct ctattccgag
    40621 cacgagatat ttctgaggga gctcatatcg aatgccgttg atgctacaca gaagttgaaa
    40681 acgctggcat cggtgggcga ttacaagggc gaggtgggcg atacaaccat cagggttaaa
    40741 ctcgataaaa aagctggaac cctcaccatc agcgatagcg gggtgggcat gacagccgac
    40801 gagattgata agtacatcaa ccagattgcc ttctcgggcg ccgaggagtt tatggagaag
    40861 tacaagaatc aggccaacgg catcatcgga cactttggcc ttgggtttta ctcatcattc
    40921 atggtgagcg acaaggtgga aattgtaacc aaatcgtggc gcgacgatgc cggagcagtt
    40981 aaatggtcat gtgacggctc acccgagttt gccattgagg aggcaacccg cagctcgcgt
    41041 ggaaccgatg ttattcttca catcagcgat gagtcgaagg aatttcttga ggagtcgcgc
    41101 attagcgagt tgctgctcaa gtactgtcgt tttctaccca ttcccattgc ctttggcacc
    41161 atcaaagagt ggaaggatgg caaggaggtt gacaccggca aggaaaaaat catcaacgaa
    41221 accaacccac tttgggtgcg taagccggtt gacctcaccg aggaggacta catgaacttt
    41281 taccgcaatc tctatccaac gcaggaggac cctctatttt atatccacct gaacgtggat
    41341 taccctttcc acctcaccgg aattctctac ttcccacgca taaaaaccaa cttcgacatt
    41401 caaaaaaaca ggatacagct ctactccaac caggtttacg ttaccgattc ggttgaaggt
    41461 attgtgcccg aatttctaac cctgctgcac ggggtgatag actcccccga tattcccctt
    41521 aacgtttcgc gaagctacct gcaaagcgat tccaacgtaa agaaaatatc gagccatatc
    41581 accaaaaagg tggccgacag gctgcaggaa atttttaaga acgaccgcga gcagttcgag
    41641 aagaagtggg acgacctgaa gctgttcatc accttcggaa tgattaccga cgagaagttc
    41701 tacgaacgtg ccgagaagtt tgcccttctc aagaatgtgg atgggaagta cttcaccctt
    41761 gaggagtacg agaagctggt taaggacaac cagaccgacc ggaataagaa tgtaatttac
    41821 ctttatgcca gcgataggga agcccagcac agctacattg aggaggctaa gaaccgtggg
    41881 tacgacgttc tgctgatgga tgggcagctc gatccgcact tcataaacac gctggagagc
    41941 aagcttaagg atacccgttt tgccagggtt gactccgacg tggttgacaa gctgatatcc
    42001 aaggaggaaa cccgtgaggt tagcctgtct gctgaagagc aggatgactt gcgggctgtt
    42061 ttcgagggtg cgcttcccga aggcgaccat tactgggttt cgtttgaaga gctgggcgaa
    42121 accgctaacc ccgttatcat tactcattcg gagttcatgc gccgaatgaa ggatatggct
    42181 gccatgagcc caaccgctag cttttacgat aaaatgcccg atagccacaa cctggtggtg
    42241 aacaccgcac acccgttggt gaaagccatt gttgaagcta agaataagca ggttggcggt
    42301 aagctgaagg agctaaacgc cggcattgag cctctaaagg ttagcgttga tgagctgcat
    42361 aaagcccaga tggaaaagaa ggaggaagaa attcctcagg ctcagaagga ggaacttgcc
    42421 gaaagggaga agcagctcga ggagctgaag gaggagaggc gcaatttact caagaatttc
    42481 ggacgtgagt acaagctggt taaggagcta accgatttgg ccatgctggc aaacaacatg
    42541 cttaaaggtg aggatttaag tcggtttgtg aagcgcagcc tcgagttgat aaaaacagaa
    42601 taaccacgtt attcattttc taccacaacc aatggcctag gggttaggtc tgaattttga
    42661 ggcctgaccc cctttttggc taaaaagcct tgatgttggt gaacccctca tcgcttgtcc
    42721 atgttgaacg ggcagaagag cagccgagcg ggttggcttg aaggaaaaat ttagcgtttt
    42781 gccttatggc atagcagaag cctaacatgt aggcatgggg ttgtctttga agtagataac
    42841 ccctggagtt acagttacaa tcaaattttt tctacggttc gggacaccct tgttttcttg
    42901 ctttcctgca gcatcgtatg gtaataattt atcatttact cgtactcgat gggcaggagt
    42961 ttgatgcctg tttttttgat ggtagagcga cagcgttgat cattaaaaca agtgttggag
    43021 ctgcatttgg ctttttccca ttttaaggag gtaggcccag ctgcacgagt actgtaaata
    43081 tttatccggt gtgcattcat aaacatgttg ctgctacagc cccgagttca gcttgctttt
    43141 attcctgccg taaaacaggt agataagcaa accaatggca aaccagataa tcagcctcag
    43201 ccatgtggca aagggcaagg cggccatttg cagaaggcag atacctgcac ccagaattgg
    43261 cacaagcgga acccaggggg ttttgaacgg gcggggaaga tcgggacgtt ttttcctcag
    43321 cacgatgatg ccgatgcaga cgataacaaa cgccaaaagg gtaccaattg aaacaagctc
    43381 gcccagaatg ctgatgggta aaacgcctgc aagtatcatt gccactgcgc cggttatgat
    43441 ggtgctaagg tagggtgtgc ggtattttgg atgaaccttg gcgaatacag gtggaagcaa
    43501 tccatccttt gccatggtga agaatattct tggctggcca agaagcatga ccagaataac
    43561 cgaggttaat ccggccagcg ctgcaatctt aatgattata cggagccagc tgtactgttc
    43621 acccattgca tcaaccgcaa gagctatagg agcaggaaca tttaagaact tgtagttgac
    43681 catgcctgtt aatacaatag ccactaagat gtagagcacg gttgagataa ccagcgaacc
    43741 cagaatacct atcggcatgt cgcgttgggg atttttagcc tcctgggcag ccgttgacac
    43801 cgcatcgaac ccgatgtagg caaaaaagat taccgctgca ccccttagaa tgccgcccca
    43861 gccaaagtgc ccccactcac caaccctttc gggcacaaat ggatgcagat ttgaactttt
    43921 aataaaggca aagcccagcg ctatgaagag aacaataacc gataccttaa tgataaccat
    43981 gatgttgttg aagttggccg attgcttgat gcccactatc agcagcatgc ttattacggc
    44041 tatgatgaac atggccggaa tattgataat agaagttacc atgggcagcg agctggcatt
    44101 aaccccctgg tttgccagga actcaacaag ctccccggtc tttggaagcc atcccgcatc
    44161 aatacccggt atatttgcag gaacctgcag cagctccatc cccctggctg ccgtgaactc
    44221 agggggtatg ttcaccccaa tttggtatag gaggctgaca atataccccg accaccccac
    44281 cgacacggtt gaagcggcaa aaaggtactc gaggattagg tcccatccaa ttacccatgc
    44341 caaaaactca cccagggttg cataggcata agtgtaagcg cttcccgaga ttggaatcat
    44401 ggaggaaaac tcggcgtagc ataaacccgc aaatgcacag gccaaacccg atatgatgaa
    44461 cgagattacg atggccgggc ctgcatactg ggcagccgcc tgacccgtta gcacaaaaat
    44521 tcctgcacca attatggcac ctatgcctag agtggtgaga ttcatcgggc caagggtttt
    44581 cttaaaccca tgttcgccgt ctccagcttc gccaagtatc ttttgaatgg gtttggtttt
    44641 aaaaaagtta gagctcatcc tcttaatgtt ttaaataaaa cctataatgc ccttctttcg
    44701 tgctattggc acgattggca ggccatcctg ctatggctat ttattgctgt tgtatggtag
    44761 attgttttag cttaggcaaa tgttgtaaaa aaaatcatac gttgaaaaaa aaatgcggaa
    44821 tatgtaaagc taaacctgaa gatatttaga aaagtctagt ttaattaggt tacaagccca
    44881 cacccgtgga aaaatcaaca agtgtttttt caaattttaa acacgctttc cagctattga
    44941 aaatgatcaa agttttgtat atccccctgc tagctgttgc gtctggttta ctaatcgtag
    45001 tgttacagaa acttgttatt cagcatgcac ctatcgcttg tttaaagggt tacctataaa
    45061 tgatggagct agcctggcat acaacatggc aacctaaaaa taaaaatacc gccgctggaa
    45121 atagtagcat aaccaacggc ggttttatcc tggggcattc ttaatgcgct aacctgcttg
    45181 caacctcggc agcatgtaaa taagcctgtg aagcagggtt cattttttta ctgctgaaac
    45241 tcgttaactt caaaggccag cttcatcacc ttgaaaactt taccatcaac atctcttaca
    45301 ggaatgtagg tttcgagtag ttcaatactt cggccaccag acgttatggt tgttcgcaac
    45361 ttcttactta cgcccgcctt caggttttcc cagaaagtcc catactcctt tttctgctta
    45421 tcgttcagta tcaggttttg tgtatgatgt gaaccgatta tttgctcccg ggggacacca
    45481 acaagcctga tgtaggaatc gtttacgttg ataacatttc cgcccagatc gtactctaca
    45541 acataagccg tgctattgat ggagttcata aactcttcaa tttcgcccga tttacggctg
    45601 acttcctcct gtgtggcttg tagctcctcc atgttctgac gcatttcttc ctcctgcgcc
    45661 ttcatctctt cggcttgcat ttgcgactgt tccagcagca ttttggtacg tgcgtttatc
    45721 ttcaccgaaa ggatggtgga agcaatgctt tcggcaacct tttctataaa ctcaacctca
    45781 tgcttctcga gcactttaaa ggaggccatt tcaacaacac ctaccacttc atcctcatgt
    45841 ttaagcggta caagaatgac acatcgaggg ttagcctcgc ctagcccgga ggtaatgttc
    45901 acgtaatcat cgggaacttc ggtaaggaat atggtttcct tttccatggc acaagcgcca
    45961 acaagtcctt ctcccagctc tatctgcttg gtcatgtatt tttttctatc ccacgcatag
    46021 gctgaaatga gctcgaagaa tggatgcatc ttatcatcat cgttgagcag gaaaatgcca
    46081 ccctggttag ctccaacata cttaaccagc gagatgatga cgttgtcgca caatttttgc
    46141 aaatcgttgt tgttttgccg gagtatttcc ccaaaatgtg catgtccctc gttggcccac
    46201 gagcgcttct ggtcctccaa cttcctgttc tcttcctcct ttgcggcatt ttttaagcta
    46261 tcgcgcatgg ttatcagcga tttgcccagc acatccgcct cgctcagcag ctcgagctgt
    46321 gtagcgtagt tcccttttcc aatatcgttg gcaaataggg ttttatggtg aagtcctgac
    46381 agtgaggtgt tgaaggcaac tgccatttct tcaatttcat cgcctgaatt aatgctaacc
    46441 atcagctctt ccgaaatttc tccccgtgca agccttttca acgcttcggt aatctttttg
    46501 attggtctgg tgagatttcc ggatacaaat agcagtacaa gtgtaatggc tatcaggcca
    46561 agcaggctaa caattattga aacccaaagg gtatgcctgg cctgtgccat gataacatca
    46621 acaggtgttg aaagcactag agcccagggg gtttttacct ttcctgccac aataggtgaa
    46681 tagcaaatca ggtgttcccg acccgaagaa tcctttatgg tatagttgaa cttttctcct
    46741 ttggcaatgc gctcggtaag ctggtatttt ttttcatcag ctggatatgc atctgctatt
    46801 ttcttgaaaa tgaactcctt gttgggatga cctgcaatga ttcctgagtt ggataccagg
    46861 tatgcgtagc taccagtaac cggcttaatt ttagaaacca tttcctgcag gttatctagt
    46921 ccaacatcgg tggcaatagc acctgcaaac tcactgttga catagactgg tgaagcgtag
    46981 gttatcatca gtttaacctc ggctttacct ttggtaacct gatcaacgta gggctcccag
    47041 attgcctcaa cgccattacg cttaaaaacg ccataaattg ggggatcacc ttccatgcta
    47101 cgctcgtcgt aaagccattt aagctggctg ccctcgcgcc atgtggtaat tacaaatcgt
    47161 ccaaaatcat tggtgtaccc gggaatgtag tacttatact cccagctgtc ccaaagcgag
    47221 tatatctgcg gattttccct gagtacaggc aggtacatat ccagaaatag cttgtgccac
    47281 tgctcctgtg gaagttcctg gtacaccgtt aaggcttgcg agagggtgcg aaccagcgat
    47341 agctcacgtt caaaaatggc cgtaacttcc ttggccgata cttctgcagt aagctgagtt
    47401 ttttccatcg cctccttgta gatggtttgc cgggaggtga ttaggatata cccgacacag
    47461 gcaatgtaga ataaccctgt tgctcccaaa atgtaaagca gtattttctg tcggatttta
    47521 agtttgcact tcatggctct ttaaaatttg ctttaaactt ataccatttt tgaatactat
    47581 gcaacaacat caggaaattt aattgcctaa acatgttaaa cttaggtcgg cggatttcgt
    47641 cttatgggaa gtaaccaaaa cccaactaga aatgacctgt tttttaaaaa aaatgacttt
    47701 atgctcagcg ctggttacct tttccatgct aggcatggca caggattata aataccagtc
    47761 tgttcctggc gacccgctaa acgccagaat ttatacctta gctaatggtc ttaaggtatt
    47821 catgtcggta agtaaggatg agccccgtat tcagacctac attgccgtta gggtgggaag
    47881 taaaaacgac cctaaggaaa caacggggct tgcccactat tttgagcaca tgatgtttaa
    47941 gggaacaccc cagtttggca cagccaactg ggagagcgag aaggtgctta ttgcaaaaat
    48001 tgactccctt tttgaggtgt accgaactaa aaccgatagc ttggagcgtg cacgtattta
    48061 ccatgtaatt gacagcattt cctacgaggc gtcaaagctc agcattccca acgagtacga
    48121 taagcttatg acggcaatag gctcaattgg cactaatgcc ggaacgtcta acgattacac
    48181 tatctacatg gagaacatac ccagcaacca gctcgagaac tgggcaaaaa ttcaggcagt
    48241 tcgctttgca aaccctgttt taagattatt ccacaccgag cttgagacag tttatgagga
    48301 aaagaacatg tcgcttacta acgatggccg aaaggcatcc gaaaccctcc tcaaggggct
    48361 tttcccaaac catccctatg ggcagcagac cacccttggc gaggcggaac acctcaaaaa
    48421 tccatcaatg aaaaacatac gcgagttcta cgccaagtac tacgttccca acaacatggc
    48481 tgttgttatg gcgggtgatt tcgaccccga tgaggctatc aggattattg acagcaattt
    48541 tggaagcctg aaacctggaa atgttccttc cttggtgtat caacccgagg tgcctttcac
    48601 ccaaccaaag gaatttgagg ttgtggggtt ggaagccgaa aacgtgatta ttggctaccg
    48661 tttcaggggt gccaactcac ccgataacga tatgctcgac cttatttcgg caatacttta
    48721 caacgggcgt gccggattaa tcgacctcaa catcaacaag aagcagctga cgcttggatg
    48781 tcaagcctac aacagggcta tggccgacta ttcaattctt caattttcag ggcgtaacaa
    48841 aaagggacag tcgctcgagg aggttaaaaa cttactgctc cagcaggttg agctgcttaa
    48901 agagggcgat tttcccgact ggatgctcga agctgccgtt aacaacgtaa agctgcgcca
    48961 aatgtcccaa ctcgaaagtg cacgcggtag ggctagtttg atgtactcag cctttttgaa
    49021 caacattgaa tgggccgatg aggttgccta catttccaga ttggaaaaaa ttaccaaaga
    49081 gcaggtggtt cgctttgcca acgataacct gggtagcaac tacgttgtgg tttataagca
    49141 acagggtaag cccgaggagg ttgccaaggt tgccaaaccg cccataactc caatctacat
    49201 caaccgcgat gccgaatcgg atttcctgaa aatgattaaa aactctaccg ttaaaccaat
    49261 tgaaccaagg tttattgact acgataagga tattaccaaa ttcaagctga acacgaacgt
    49321 agaggtgctt tataccaaga atgccgaaaa cagcaccttc aacctgactt tatactaccc
    49381 atttggaaga ttggctaatc ctttgctaac tcatgcagcc gactacatcc agctgcttgg
    49441 aacttcaaag cttaccgccg aacagctaag ccaggagttc tacaagctag cttgcaactt
    49501 caacatttct gtcagggacg aggagatgct aatttcgttg acgggcctga gcgataatgc
    49561 cgataaagct tttgagctga tggaaaacct ccttgccgat tgccagccca acagcgatgc
    49621 cctctctatg tacattacca accagctaaa atcgagagac gatgccaaga aacggcagca
    49681 aagtgtgttt agcgcattgg ttagctatgc tacttatgga gaggaaaacc cacaacgctt
    49741 aattctctca aatgaagagt tgaagggtct tactgcccaa ccgctggttg acctaatcag
    49801 ggagtttacc tcatacccgc acatcattct ttactatgga cctcaggatg ttaaggggtt
    49861 gaaaaaaatg ctcaccgaaa agcatattca acctaaaaag tacaaatcgt tgccggtgaa
    49921 caaaaagttt gaggagactt caacaactac cgaaagggtg ctctttgtaa actacgatgc
    49981 caagcaatcg taccttcaaa ccattagtaa gggcgagctt tacagcgaca acctttatcc
    50041 tgtggtaacc ctttacaacg aatattttgg tggtggtatg aatgccattg ttttccagga
    50101 aatgcgtgag aaacgtggct tggcctacac tgcacgttct ttgtacagta ttcccgatga
    50161 gcccaatagg gtattcatga acacaagctt tattgcaacg cagaacgaca aggttattga
    50221 tgcttttaat gctttcaacg agcttttcaa caacatgccc gaagcggaaa ttggttttaa
    50281 cctggcaaag gaatcgctca tctccaacat acgttcacag cgaatcatca ggatggcctt
    50341 actttacaac tacctagatg cccgtcgaat gggctggaat cgcgatatgc ggcaaaccct
    50401 ttttgaggtt attccgggaa tgacccttaa cgatgtgcgc ttattccagc aaacccatgt
    50461 aaagggtaag cctaaaacct acgttattct tggtaaggag tctgacatgg atttcaatag
    50521 actttcgtcg ctgtttggac ctgttaccaa gctctcgctt gaggatattt ttggctacta
    50581 gaatgggaaa acaaaaaagc cgattgttca atcggctttt tttacaccta gcctatcatg
    50641 actgcctaag cgcagggtta gaacgggtgt ataagtgcaa gctttttaga ataactttac
    50701 tagtacatac aaattgcgag tattatgttg aaaaggggta aaatgttgct gttactggcg
    50761 cttgtttatt ttaccggcag tggccaggat aggatgacag ggcttaactt tgccacccgt
    50821 tcggaggtga ttgcgagtca cggtatggcc tgtaccagcc aaccgttggc aactcaggtg
    50881 gccatagata ttctgaaaaa gggcggaact gctgtggatg ctgccatagc agctaatgcc
    50941 atgcttggtc tggttgaacc aaccggttcg ggcattgggg gtgacctttt cgccattgta
    51001 tgggatgcca aaacgcaaaa actctatggg ttaaatgcca gcggccgttc gcccaagtcg
    51061 ctatcacttg agtatttcaa ggagcatgga ataaagcata tccctgccat tggcccgctg
    51121 ccggtatcgg tgcctggttg cgtggatggc tggtttgagc tgcacagtag atttggcagg
    51181 ctgcccatga aggtaatcct tgaaccaaca attggctatg cccgggaggg atttcctgta
    51241 tcagagctaa ttgcctacta ctggaagggt aatgccgaaa ggcttaagca gttcgaaggt
    51301 tttgctgatg tttacctgcc caacgggaaa gcgcctgcaa agggggaaat attcaagaat
    51361 ccctacctgg caaatacctt ggaaatgatt gcaaagaatg gtcgtgacga attctaccgt
    51421 ggcggcattg cccggaccat tgatgattac atgaagagga atggtggatt tttgtcctac
    51481 aacgatttag cctcacaccg ctcggaatgg gttgaacccg tttcaacctc ataccggggc
    51541 tacgatgttt gggagcttcc cccaaacggc cagggcattg caacgctcga gatgctaaac
    51601 atcctcgaaa atttcgatat tgcctcaatg ggctttggct cggctgacta catgcacctt
    51661 tttgttgaag caaagaaact cgccttcgag gatagggcac gttactatgc cgatcctgat
    51721 tttactgacg ttccatatat gagtctgctt tcgaaggact acgcaaaaaa tcgtgctgcc
    51781 cttattgata tgaaccgtgc agcaagaagt tacgatgcgg gtgagttgca aaccggcaac
    51841 accgtttacc tcaccgttgc cgacagcgag ggaaacatgg tttcgctcat tcagagcaat
    51901 tacaggggaa tgggttcggg aatgactcct acaggtctgg gttttgtgct gcaggacagg
    51961 ggtgagctgt tctcgcttga ggaaggacat cccaatgttt tccaacccgg gaaaaggcca
    52021 tttcacacca ttattcctgg ttttattaca aagagtggaa ggccatgggt aagctttggg
    52081 gttatgggtg gcggaatgca accccagggg catgttcaag ttattgttaa ccttatcgac
    52141 tttggtatga acttacaaga ggcaggcgat gcaccgcgaa tccagcacga gggtagctcc
    52201 gagccaacgg gtgagaggat gaacgatggc ggtaacgttt ttctggaatc tggctttgac
    52261 tatcaggaaa tccgtaaact tctcggcaaa ggacacagga tttcatacag ccttgacggt
    52321 tacggcggat accaggctat aatgtacgat gaacggaata gggtttacgt tggggcttcg
    52381 gagtcgagaa aagatgggca agccgcagga tactaatcaa aaaagaggct taaggcctct
    52441 tttttgatta gtgacgcaat ataactactt gtgaatgata ggatcggggt tgccatcggg
    52501 atcgttgagc acgtatccat cggttcgggg tgagccaagc agggttactc cacaagcatg
    52561 aggtgaagcc atagaagtgc cgctaagggt tgcgtattta cccgacttgt atgtcgagta
    52621 gatgcttact ccgggagcac agtagtctat aggcggattc ccatagttgg agaagtatgc
    52681 aaatttatcg gcattatcca ttgctgagat ggtgaagatg ttagacccat tcacccgtgc
    52741 aggagagcta aggttggcat tagcactttc attaccagcg gctaacgaaa acttaatccc
    52801 cttgttagat gctgccagca cagcatcgtc gagtgcctga taggtggggc ctcctaggct
    52861 catgttggct acatcacccg atttgccagc agaggcaaca tagttaacac cggaaataat
    52921 ggtggagtaa gctccgcttc cactacggtc gagaaccttt acagccacta caggcgctcc
    52981 tgcagcaacc ccaacaacgc caatggtgtt attttttgca gcaactgtcc ctgcaacatg
    53041 cgagccatgc ccgttatcgt catcgggagt tcttccacgg ggtacgaaag tcttgctgcg
    53101 agaaacatcc acatttaaat cgggatggtc taaatcaata cctgtgtcga ttatccatgc
    53161 cacgttggtt cctgtatagt tggtagcgcc tccaacacgg gttatacccc atggagtggt
    53221 ttggcccttc gctgtgggat cggagattga ctcaaccttg tagtccagca ctatccagcg
    53281 atcaggttct atataggcaa ctctcgaatc cctataaatc gcatttacct gtagctcgtt
    53341 gagttcggcg gaaaaacctt ttatggaagc tccatacacc tggtcaatct taccggatgg
    53401 tacagcataa acactcaaaa aggttgatgc ttcagccagc gctttagcat ggctttcttc
    53461 ataacttacc tgttgcacct tgtcaaccct tgtgctgaat gtatcctgtg taaataccac
    53521 aataaatttc cccgggatga tatcgccatt cttcagctcc tgcttcgttg agagcgggtt
    53581 ggtttcatcc gaaaaatttt cttccttttg gcaggcagtc agtagcaacc cgccaagcgc
    53641 cagagcataa acagattttc ttaacatggt taaatggttt taggttagta ttcaggtgca
    53701 aattatggct atattacata aaagtcaagt gctcagaaag ttttaactat ttttttcggc
    53761 atttgacgga tttaaatttc aaataatacg cagccaatca catagcttta aacctttaac
    53821 gcctaccggc tcacggcttt caactcaatt ttttttagca cacccgggtt acctgtaaga
    53881 acaatttcac tatcttttaa ccatctaaaa tccattttac ttaaaacgat aacaggttat
    53941 ggtagagctg gttaaaggag acattacgtt aatggatgtg gatgcgatag taaatgccgc
    54001 caacgaatcg ttgcttggcg gtggaggagt tgatggcgct atccatcgtg ctgcgggacc
    54061 gcagctactt gcagaatgcc gaacgttggg tggatgccct actggtgagg cacgcatcac
    54121 tagaggctac aatcttaaag ctaggtacat aattcatact gtaggccctg tttggcatgg
    54181 cggatcgcgg ggtgaggcta agcttctggc ctcagcctat tggaacagct tacagcttgc
    54241 agctgattac tcgctgaaaa gtattgcatt tcctaacatt agcaccggtg tttacggtta
    54301 tcccaaaagg gaggccgccg aaatagccac tagaaccgta aacgaattca aacagcaaaa
    54361 cctacatccc gagagggttg tttttgtctg ctttgatgac gatagctatc agatatacaa
    54421 ggagatgctt aagcgttaaa tgttgatgga gtatttctcc agccttcggt cgagcgattg
    54481 acgggtgatg ttgagcagct gtgcagcctc tgttttcttg tagcctgtgc gttccagggc
    54541 cttcataatc acaaattttt caatgaggtc caggtcgaat acgtcatcgc ctatgccggg
    54601 tagttgggtg cccgcttctc cggtttggct tgttaagccc ggaaaatttt tcaggctaag
    54661 cttgctacca tcgcaaagaa taatggcacg ctcaaccatg ttgcgcagct ccctgatgtt
    54721 tccggggaaa ttgtagccca tcattaccct ttgcaccgaa gggtctattt cggaaatcct
    54781 tttgcccagc ttacgggcaa aaaactttac atagtagtcc agaagtactg gaatatcctc
    54841 cctacgttcc ctcagaggtg gaatgtggat ttcgaatgta tttaaccggt agtagagatc
    54901 gctacggagc ttgccggttt cgagctgtgt gaaaacgttc tggttggtgg cagcaattat
    54961 gcgcacgtca acaggaatat caacgcttga gccaattctg cgaaccttac gttcttccag
    55021 caccctgagt agcttgctct gcatggcagg aagcatttca actatttcgt ccagaaagag
    55081 ggtgcccccg ttggctacct cgaaccaacc ggctttgtcc tcgttggcac ctgtgtagga
    55141 acctttttta tgcccaaaaa attcactttc aaaaagcgat tcgggaatgg ccgagcagtt
    55201 tactgcgtag aagtatgagt cctttctatt gctgagataa tgaattccac gggcaaccag
    55261 ttctttccca gctccgcttt caccggtaat cagcactgag gtattttctg ttttggccac
    55321 cctacccata aggttaacaa catggcttat ggcgggtgat gttcccagaa tctcgttacc
    55381 cacactttca atcagctcct tcgaaataag cgagtaggtc tggttgattt ccttaagttg
    55441 gttgctgatg gtaatgtagc gcttggttcg ctggatggcc gctcgcatgt cgagtaatcg
    55501 gaaaggtttc gggaaaaact ctactgcacc cagccgcatg gcctgtatta cagcctccat
    55561 gtcgccatgt ccggtaatca taattacttc aatgtcgggg tactcctcct taacacgccg
    55621 gaggacctca aagccatcca tttcgggaag ttttatatcc aggatcatga tgtcaacttc
    55681 ctgctgggtg agcatctcga atgctgctga tggcctatcg gagagcaata ccgtgaaacc
    55741 atcgttttcg aggaattcct ttatttcatc ccgaaaaacc ttttcgtcgt ccagcactaa
    55801 gattcttatt tccttcatgc ctatctacat tttgggaagt gagatttcaa agcgggtata
    55861 ttcaccctca acgctttcaa ctttaatgtc acctagtaaa tccttaatga atgcatagga
    55921 taccgaaagc ccaagcccgg ttcccctttc ctcgcttttg gttgtaaaaa atgggtcgaa
    55981 tatcttgtcg atgattttgt cgggaatgcc catgccgtta tcccgaacgc ttaggtaaac
    56041 ctgattgtta tcctgccatg ttttaatctc aatcagtttt gtgtactggt ggttggcgtg
    56101 ttttttccct ttttcgtcaa cagcatactt ggcgttggaa agtaggttaa ggataacctg
    56161 ctccagcttg tacttgtcgg cggtaatgct accgatgttt tcgtcaaggg taacttccat
    56221 ttttaccccg tggttctcgt actgggtaga taccatgaaa agggagctgt taaccacatc
    56281 gtttatgttg atttgtgtga aggtaatagg cttttgagct cgtgaaaaat agcgaatatg
    56341 gtcaataatt tttttaatac gctccacatt ttcaaaaagt gaggatattt tttctttcat
    56401 gaactcatcg ttgtagcttt tatcctgcac cttcagcagg atgttgtcga gccccattga
    56461 aatgcccccc aggggttggt ttatttcatg ggcaatgccc gctgcaagcg taccaaggga
    56521 ctcaagtttc gacttttggg caagcagtag ctgttgtttt tcctgttttt ttatctcctg
    56581 aatcatcctg cgctcaagct cgcggttcaa atcggtgatg cgctgttccg tttcaaggcg
    56641 tttggcaatt tccttctgct tagaccgaaa gagtatgatt aaaagaacaa ttagggtgat
    56701 gaaaatgatg atgatgagaa tcagtattcg cgtttggtac ctggttttgt taagctgcag
    56761 gttctgaatc tcggaattct gcctcagcag ctcattctcc ttctctttct gggaaatctc
    56821 gtagaggatt ttaagtttag ccagcttctg tcctgtttcc tcgaagtaga tggagtcgcg
    56881 tgtttcaacc cacattttat agaactcgag ggcatcccta tacctgccct gaccctcgtt
    56941 taggatggcc aggtactggt aaacgtcggc taccgactgg gtgaggttgt tttgccgggc
    57001 aattgccaag gctctattta ggtatttaga agcgttggaa atatcgccgc tgctgaggta
    57061 gcatctcgaa atttttacca gggattcggt gatagcggga atatttttta acttttgtgc
    57121 cacggtgagt tgctggttgt agtagtagag ggcttgagta ggatttcctg aatcgaggta
    57181 aatatcagca attccctgaa ggccttgaat catgcctacc gtgtcggaaa ttttgttgct
    57241 gatggcgtat gaggctgtaa agtaaaagtt ggcgctatcg ggtttttgta gaaggtagta
    57301 ggtgagcgct atttcctgaa gtgcattgga gtagctaagt gaatcaaaat tatttataga
    57361 aatttctgca gcttgtttaa agttgcttag gctttctacc agttcccccg aattcttttt
    57421 tacctgtccc agcttaaaga aggcgtcaga tttgattttt tccttcaaaa gcacctttcg
    57481 gcccatcggg ttgtcaacta ttgccagcac cttgctgtac atttcagctg attttctata
    57541 gttgccttgt ttctggtaaa gttcacccag cagttctagc aggtcgatat acaggtttat
    57601 gttgttgatg ttttcgtcag aggcaagccc cctttcaagg gtggaaatgg ccagctcggg
    57661 atttttctca gcatcataca ccctcccaag cccaatgagt atcttgtact ttgcatcgga
    57721 gttgccaagt tggtcataaa agttgagagc cctattatag tagcgtgatg aattttcata
    57781 ctgtccaagg gtaaagttcg actcggctaa aatcaggaga gcttcgattt caaatcgggt
    57841 atcgcccagc ttttttgcca gcactagagc ctgtagccca ttgtccaatg ccttatctgg
    57901 atttttgaca acattgtccc ttccctcgga tatcaggttg gccagaactt tttcctccga
    57961 tttggaggta aggctcgaat ccgggctgac ctgggcttga agagtgggga attgcataaa
    58021 aagccagaag gccaaaacaa tcgtcgtatg cttcccgaac cggctcatag ggtggttatt
    58081 gcagcactaa agatacccaa atttaaaaaa atggctactt ttggatcgca ttttttgcct
    58141 gagggacgaa ttcatcggag tactatcggt ctattgctaa tgtttttgat cttccactgt
    58201 tggtgaagtt ttactgtttt tttattcatc ctgttgagtt atgtgcatta cgcacagtag
    58261 gtttaaagtg aggcaaccag aaacggcttg tttatgtgag catgcttcat gtgttcaacg
    58321 gcgttaatac gttttttaag aaatgagtag gagtagattg taggatattt actatttaaa
    58381 gtgtttaata tttggtgctg atgagtacaa ttctttctgt ctatggtaaa aacaattagg
    58441 aattatcttt cgctggttaa gtttagccac actgtatttg cattgccgtt tgcagtaatt
    58501 ggattttttt tggcctatcg gcaaacccct cagaatgttg atgtttggtt gtttgtgctg
    58561 gtggtgctct gtatggtttt tgcccgtaat gccgccatga gctttaaccg gctagtcgac
    58621 cgtcgtttcg acgccattaa tccaaggacg caaaacagag aaattcctcg cggcataatt
    58681 acctccaggg ctacagccgt gtttgttacg gtgaatgtgt tgcttttcat tgcaaccacc
    58741 tacttcatca accgcacggt gtttttcctt tcgccaatag ctttggcaat cgtgcttctg
    58801 tacagctaca ccaagcgatt taccatgttt tgccattttg tgcttggtct tggattggcc
    58861 ctggcgccta taggtgccta cttgtcagtt acccccaggt tcgagctgtt gccgttactt
    58921 tactcggtta tcgtgctatt ctgggtatcc ggttttgata tcatgtatgc cctgcaggat
    58981 gaagattttg ataagcggga aaacctaaag tcgttgccgg cagcactggg cgttaaaaaa
    59041 tctcttttgg tggccaacct tctacatttt gttgcaggag ctgtcgtagt tctcattggt
    59101 atcctatatc actccgatgt atggtactgg gtgggtgcag ccctttttat cgccctgctg
    59161 atataccagc atctaatagt taaacctacc gatatatcga gggttaacat ggctttcgct
    59221 accacaaacg gtctggcaag cattatttat gctacgttta atgttatcag tatttttttc
    59281 taacttgcac aagctagttt taaaggacat tttcaactaa attttactca aaatcacaga
    59341 agtatgatga aaaaagtatt tttactcgca gctacagcag caagtattct tgcctgctca
    59401 tgcggaacaa agagcagttc tgacaaccag caggcaaaag ccgattcaac agcagcgtca
    59461 attgttgagg ttagcattga ttctctcatc tcaaacccac aggcatttgc caaccaaacc
    59521 gtaaagttta ctgccaaggt tgatcatgtt tgcatgcata gcggccaaaa gcttacgctc
    59581 atgggcagcg attcgggcag caccttcaag gtaatggcaa ccgatgccgt tccccagttc
    59641 gatcagaatc tcaacggaac cactgttgag gttgttggcg tggtgcaggg tgcagcacct
    59701 gaagatgtgg aatcatgcag cgaagaggaa ggtggacagc agaaagcaaa aaaactcgac
    59761 tatgcagttt tctgccaaaa agtaagggcc ctctagcatt ctaccttaat taaactatac
    59821 tccggtggca atccttcacg ccttatggtg gaggaggata cctgccggtt tttttatacc
    59881 agggaaaata tataagttaa tcgttcatga acatgcgcaa gctgctcagg gtgctgcacc
    59941 gtgattttgg ttactttttc acgggaatga ctcttatata ctgtgtaacc ggaattttgc
    60001 tcaaccatac ccgcgactgg aacccctact actccattga tattcgtaag gtggagttta
    60061 atctcccact cgacacatca ttaattaaca aggagttgct gacttctaag ttggaaccta
    60121 ttggtgtaca ggggctaagg tcgttctact accctaaacc cggaacagtc aagctatttg
    60181 tttccgatgg aacggttact tcagatctag ggagcggtag cgctctggtg gaaagaagct
    60241 accgcaggcc tgttctctac cagcttaaca tgctgcataa aaatctaccc aaatcatggt
    60301 ggacttggtt cagcgatatt tttgccatag ccctgatggc aattactata tcggggcttt
    60361 tcctggtaaa agggaagaat ggcattaccc atcgtggtgt tattctcatt attgctggtt
    60421 tagcacttcc ccttgtggcc ataattctcc tctaaacatt tttagctggg ttgataggaa
    60481 tatttagcta atgttgacta aatttgcttc tacaagtaag catcatatcg gcattcagca
    60541 taatgaatta ttgcgacctt cattttaaat tttctgccaa gtacgtattc ctgaacatca
    60601 agaacaggga taactttttc taatgccgtc atttgcatag ccatcccccg gctatgcagc
    60661 actgtttaag ttatccctaa gttttaattt ttttccgcta aaaaattatc gatatggaac
    60721 taccatttgc agaatcgtac aaaattaaga tggttgagcc tatacaccgg agcacccgaa
    60781 gcgaaaggga gcgttggatt aaggaggcca agtacaacat gtttaacctg aagagcgagc
    60841 aggtttttat tgatttgctc accgattccg gaaccggtgc catgagcgac aggcagtgga
    60901 gtgcgctcat gatgggtgac gagagctatg cgggagcttc ctcatactac aaccttaaaa
    60961 gcgccatcca gaatattctg ggatttgagc actttttgcc aacccatcag gggagagctg
    61021 ccgagaatgt ccttttctcc gtgatggtga aagagggcga ctacattcct ggcaactcgc
    61081 agtttgatac caccaagggg catattgaat ttagaaaagc cgaggcggtt gactgcacca
    61141 ttgatgaggc agagaataca gagatttacc acccttttaa aggaaatgtt gacccccaga
    61201 aacttgatga ctttatccgt acccacgccg acagggttcc atttgtcatt atcaccatta
    61261 ccaataactc agcaggagga cagccagtat caatgcaaaa cctgaaggat gcccgaaaaa
    61321 tttgcgataa gtacggtaag ccgctggtga ttgattcggc cagatttgct gagaatgcct
    61381 actttatcaa ggttcgtgaa aagggttatg ccgataaaac cattaaggag attgtgcgcg
    61441 aaatgttctc gtatgccgat gctatgacca tgagcagcaa gaaggatgcc attgttaata
    61501 taggcggatt tattgcaatg cgcagcaagg agctttggca gcaatgctca accttcaaca
    61561 ttatgttcga gggatttgtt acctacgggg gcatgtcggg gcgtgatatg aatgccctgg
    61621 cggttggtct tgatgaagga acggagttcg actacctcga aacacgcatt aaacaggtgg
    61681 agtaccttgg taacaggctg aaggattttg gtattccttt ccagttccct gctggtggac
    61741 atgccatttt tgttgatgcc aacaaggttc tggctaatgt tcccagggag cagtttcgtg
    61801 cccaaacgct ggctgttgag ctctacctcg aggcaggcat tcggggtgtt gagattggtg
    61861 ccctgatgtc ggatagagac ccggttaccc gcgaggtaag aatgcccaag ctggaacttc
    61921 tcaggttggc tatcccccgc agggtttaca ccaataacca tatagacgtg gtggcggttg
    61981 cccttaagaa tgttttcgac cgtagattag agattaacaa aggcttgcga ataacatggg
    62041 aagccccaat tatgcgccat tttaccgttc agctcgatag agcacgataa tctttttcaa
    62101 ctcttttgcc attggcttgt tctgtagtgg atcaggccaa tggtttttta tttatcattt
    62161 accccaaaaa tagcctactt ttgcaattca tttctatgca atgagggatt ttacaaccgg
    62221 tagcgaaacc caacatattt taaggtttgc aacacccatg ctgataggcg atatttttca
    62281 gcagctctac aacgtggttg atagcattat tgttggtcgc tttattggcg ataaggcttt
    62341 ggcggctgtt ggagcatcgt ttccggtaat ctttgttatt attgcccttg tgcttggcat
    62401 tggcataggt tctacggttg tcatctcgca gtactttggt gcgcgtaact tcgatatggt
    62461 gaagcgtgca gctaacacca tctatatttt tcttctgatt tcgggcgtgg tggttaccgc
    62521 cattgggctg atggcaagcg gagccatttt taggatgctt cacctgcctg aagaactcat
    62581 gaagccagct aggacctacc tcaacatttt tatgctgggc atggtggtta tgtttgcctt
    62641 caacgctata gccagcattc ttaggggaat tggcgattct aaaactcctt tgtattttct
    62701 tcttatttca acgcttacca atgttggatt ggtgctactt tttgtggtaa aatttcgatg
    62761 gggcatagcc ggtgctgcat gggcaactgt catttctcag gttgtggcgt ttattatagc
    62821 cttaatctac ttaaaccaca gccagcacat gcttagcata aacctacgaa ccatgaagtt
    62881 cgaccgccaa attttcaggc agattgttaa aattgggtta cccaccggaa tacagcaaac
    62941 tatggtagcc cttggctcaa tggcgatgat gggaattgtt aattcttttg gaaccaccgt
    63001 tattgcggct tttactgttg ctagccgttt ggatgcacta attgcaaccc ctataatgga
    63061 ttttgccgcc gctctttcgg gatttgtggg gcaaaacatt ggtgccaaca agattgacag
    63121 ggtaaaaagg gggctacggt caaccctgaa aatttccatt agcttttgtg ttgtcaacat
    63181 tgccattatt ctgatttttg gccacagcct gatggggata ttcactaaaa gttccgatgt
    63241 gattgccata ggttaccagt acctgctggt tgttagcttg ttctacataa tttttgccgt
    63301 aatgttttcc atcaacgggt tgctcagagg ggtgggcgct gctgttgttc ccatgtacat
    63361 caccctgctg tcgctctgga tttttcgaat ccctcttgcc tacctgttga gcaaaacctt
    63421 tggtatgggt gcattgggta tctggtggtc tattcctatt ggttggacga tgggggccat
    63481 tgctgcagta gcctactatc gccatggaag ctggaagaat aaatccctgg taaaggtacg
    63541 ccagcaagct gcggattaga gttgagatgg tagcaagtaa gtagctccga aaaccaaaag
    63601 gttgaatatc tcaccgaaac aaaactattt gccatttttg cttggattga attccatttt
    63661 ttatcctaaa gctggttaat aaattagatg ttgatggctg tttcctaaaa attttatatt
    63721 tgtaggagca atataatggc attccattaa atactcatta agctacactg gttgataatg
    63781 atggtaattc atgtcaggca acatgatttc ataaacgggt gatggagttc ttgagttcta
    63841 taaaactatt gtgggtgctt ttcaatctgg gcaggtactt ttccttgcgt caatagtttt
    63901 tatttattgg tttgctgccg ggtaaattct atcatataaa ctttttaaaa catgctaaag
    63961 gactatttaa gcagaattca agataccatt aacaagggag atgccaggga ggagagctac
    64021 tactcacatc tagatagcct gattaaagaa tttgcaatag aaaatggcat taaaaagatt
    64081 gatgtaacaa tacttcccaa gaaaacagaa gcggggaatc ccgatttccg agtatgggac
    64141 gggaagaacc atataaccgg gtatattgag gcaaaggatc cttcggttga caatcttgat
    64201 aggattgagg aatcggagca gctaacacga taccggagca cctttcccaa cttaattctg
    64261 acgaattttt acgagtttag gctatacagg gatggcgaac tggtaagaaa ggttacgatt
    64321 ggccgacaga ggattgccca aaagttgaaa acagctccac cggtggagaa cgagaaagaa
    64381 ttcaacgacc tgcttggcac cttcttctcc ttttccctac cacaggtacg tagcgcaaag
    64441 gcgcttgcac aggagctggc taaacgtacg cgtttcctgc gcgatgaggt ggttagcatt
    64501 gagctggcag aggagggaag gattggaaaa agggatatta tggggttcta tgaggctttt
    64561 aggaagtact tgataggcac tttaacggag cagtcctttg cccatctcta aatatcaagg
    64621 tgaatctgaa aatgacagaa ttgaagggat tgcct
//
LOCUS       DAOSHM010000008        64053 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_358_length_64053_cov_20.611878, whole genome shotgun sequence.
ACCESSION   DAOSHM010000008 DAOSHM010000000
VERSION     DAOSHM010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 64053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 64053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64053
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_358_length_64053_cov_20.611878"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            213..626
                     /gene="rsfS"
                     /locus_tag="PK839_02320"
     CDS             213..626
                     /gene="rsfS"
                     /locus_tag="PK839_02320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HOG71601.1"
                     /translation="MAGKKGNADETLQLLKCIVDAIEEKKGLDVVSIEIGELQNAVCD
                     YFVICHANSTTQVDAIAGNIEFKTLELQNQRVYRSSGYENAIWIVLDYIDIVVHVFLS
                     EWRNYYKIEELWADGKLCRYSDPDDDFSSMQPATF"
     gene            679..2706
                     /gene="ftsH"
                     /locus_tag="PK839_02325"
     CDS             679..2706
                     /gene="ftsH"
                     /locus_tag="PK839_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008150370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOG71602.1"
                     /translation="MSNNKNSGNQMPGDRKGQVPKPPRFNVYWIYILIILGVFLIQFF
                     FSGKSPVVTSWQEVKNQMLANDEVSKLVVVRNMGKVEVYLKEDKLTKYKDRLGEGMGA
                     VPKSGPHFYFTIGSVETFEKQLNEAQQGKPDAQIIYPEYTDQHNYWGDILSWVIPILL
                     IVGLWAFFFRRMTKNAPGGAAGPGNIFSVGKSKAQLFDKESNQKIDFKDVAGLEEAKV
                     EVMEIVDFLKNPKKYTELGGKIPKGVLLVGPPGTGKTLLAKAVAGEANVPFFSMSGSD
                     FVEMFVGVGASRVRDLFKQAKEKSPCIIFIDEIDAIGRARGRNANFSGNDERENTLNQ
                     LLTEMDGFASNQGVIIIAATNRADVLDKALLRAGRFDRQIHVELPDYKERLDIFKVHL
                     RPLKLEGNVDITFLAKQTPGFSGADIANVCNEAALIAARKNKKQVERQDFLDAIDRII
                     GGLEKKNKIISKDEKRAIAYHEAGHATVSWLLEYANPLVKVSIIPRGISLGAAWYLPE
                     ERQITTTEQLLDEMCATLGGRAAEELVFGKISTGALNDLERVTKQAYAMITYFGMGDN
                     FRNISYYDSTGQSEFTFSKPYSEKTAEQIDEEVKAIIDKAYNRAKEILRENSDGHVKL
                     AELLLEREVIFSEDLEKIFGPRKSLSREEELAKEFSDELKKTQSPNESDNG"
     gene            2699..2902
                     /locus_tag="PK839_02330"
     CDS             2699..2902
                     /locus_tag="PK839_02330"
                     /inference="COORDINATES: protein motif:HMM:NF020967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HOG71603.1"
                     /translation="MDDSWIKVFSTSLPWQAEMAKQVLEENGIEAVVINQQDSSFFFG
                     EAMVYVEGKWEDQAKELLKGIGS"
     gene            2899..3726
                     /locus_tag="PK839_02335"
     CDS             2899..3726
                     /locus_tag="PK839_02335"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HOG71604.1"
                     /translation="MTEFLKRTISGLIFVVTITGALVIGHVAFFIVFLGLMVATQYEF
                     YKMTFKTHLRPQILLGLFIGIALFSWSFLYSLGKVEQITIFGFIPLLGGIFVVELYRN
                     QHKPIQNIAVTLLGIIYIAVPLSLVNFIVINGSSTRISYSPNILLGLLFLIWSNDTGA
                     YLLGVSMGKHKLFPRISPKKTWEGFVGGLLLTMIVSWVDAIIFPEVAFKHWLVIGVIT
                     AIVGAFGDLVESMFKRSVGVKDSGKFLPSHGGFLDRFDAFLLVIPFVYAYLEVMMLI"
     gene            3739..4386
                     /locus_tag="PK839_02340"
     CDS             3739..4386
                     /locus_tag="PK839_02340"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HOG71605.1"
                     /translation="MKIHREGLVFLAVSLIILAALNVAVGLILPEVTYFLAPLSLLIL
                     LFLVRFFRIPMRGFSPNNEYIYSPADGTVVAIEEVEENEYLKTRCIQVSVFMSIWNVH
                     INWFPTTGTIKYYKYHPGDYLVAWHPKSSSHNERTTVVVERSDGTKILIRQIAGALAR
                     RIVCYAKPGQSIEQGSELGFIKFGSRVDLFLPLNANVEVKLNDRVVGKQTVIARL"
     gene            complement(4392..4913)
                     /locus_tag="PK839_02345"
     CDS             complement(4392..4913)
                     /locus_tag="PK839_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG71606.1"
                     /translation="MNTAVKIACYTVLLLVAGNSCNSVSKVTTTNQGISKQEFLKAPF
                     GHDESIQAFTTQLPARTKITRFARSNVHEPSRTDTIYKFSYKKSELFVYKTYFNREML
                     MAGKIVNPEIKLVNGISVGLTRDQLLKAISNINESSSDTLVISNEYYGRTFNFIFNNK
                     GKVREIVFSSYAD"
     gene            complement(4979..5902)
                     /locus_tag="PK839_02350"
     CDS             complement(4979..5902)
                     /locus_tag="PK839_02350"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOG71607.1"
                     /translation="MDFISSHTGELAALLTAVFWTVTSLAFESASKTVGSLSVNFIRL
                     VLAFIFLSLYSLFARGLLLPTDAGGHQWLWLSLSGLVGFVMGDLFLFQSYVLVGARIS
                     MLIMSLSPPIAAGIGWLSMGETLTLKQALGMLLIFIGIAMVVLRSEKTPNGSKIIGRK
                     IKFGYSIPGLLLAFGGAVGQAGGLVLSKYGMAGYDVVASVQIRVITGIVGFAAVLLFM
                     HKSGNLLVAVKNAGAMRRITVGSFFGPFLGVSFSLLAVRYTSTGVASALMSIVPVLII
                     APSAVLLKEKITFREVAGAIVSVVGVVTFFL"
     gene            6105..7208
                     /locus_tag="PK839_02355"
     CDS             6105..7208
                     /locus_tag="PK839_02355"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOG71608.1"
                     /translation="MNKLVKYIIIVFVVAAVAFLLWYFSSIVAYILISAVLSLMGKPI
                     VDGVCRLKIRNWHPPKAVGATCALIVIWVLFVAFFSFMIPLVVSQFNSLSGVDVGTLV
                     NRFAHPIAGLQEFIQNYLPASAKEFSIEEYLTSKISTFFTTSMIANLFSSTANFVVNL
                     GIALFAISFITFFFLKDDTLFYNGVILLFPSRFEQQLSHALSSINRLLRRYFIGLLIE
                     STLIMLLDTLWLVLIGLHFQTAIVIGLIIGILNVIPYVGPLIGIIIGTLIGLASQLQL
                     ISATELLPLTLWMVGAMLATKLIDDAVFQPVIYGNSVKSHPLEIFLVILIAGSLAGVL
                     GMLLAIPAYTVLRVFAKEFFNNFRLVQKITEKI"
     gene            7328..8269
                     /locus_tag="PK839_02360"
     CDS             7328..8269
                     /locus_tag="PK839_02360"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOG71609.1"
                     /translation="MLVNSHLKNGFLLLAGLLVSNAAIAQENLEADSIFTWKLVELTN
                     PSSHSCIQFIPRVDITPVSWDFGDGSPASADVSPVHTYTYGSWSDSVEVTLTYTAGGA
                     NTVKTRQIPLSPAYFHCIHDSNLGQAASFKYIFRNAYMIDNNPGNLGNLHFSWTIDSE
                     PLAENTFSNPDLGQWPNIYYTFLNGGTHQVKLEVYNTTNATEVAGFTHTLTVTPDLSA
                     GVVKLENIPNVFTPNGDGVNDTFTVNTSGTGWFVLRILSRSGGLLYKSESSTLIWDGR
                     NSQGEELPEGIYYYIIEDTTGQYETATGFIYIFRGKK"
     gene            8421..9170
                     /locus_tag="PK839_02365"
     CDS             8421..9170
                     /locus_tag="PK839_02365"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HOG71610.1"
                     /translation="MALLEEFEKQGNWLFRYRGVLPLIILAVGAFIYIRAELNPSPSS
                     FMEVNSYKSIYEISCLLVSLFGLAIRVYTIGHTPSKTSGRNIKEQVAESLNTTGIYSV
                     VRHPLYLGNFFMWLGPAMLTANFWFIVSFCLAYWIYYERIMFAEEQFLRRKFGEAYTE
                     WASRVPAFIPCFKHFKKPCHSFCTRKVVAKEKNGLLAVFLIFCSFNVVGELVHKGKHH
                     FNMIFIGMCILSIVLYIVVKIFRKKINLVKQ"
     gene            complement(9215..9853)
                     /locus_tag="PK839_02370"
     CDS             complement(9215..9853)
                     /locus_tag="PK839_02370"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOG71611.1"
                     /translation="MAKVDNDMEEYLSRHTSPMADTLMELTRFTYLSTCNPRMISGPI
                     QGKFLQLLCNISRPNRILEVGTFTGYTSICMALGSPPHTVIDTIEVNDELEETIQTYT
                     SMAGVDSKIRLHLGDACQIIPTLDYTYDFAYIDGDKREYPEYYKLVLPKVNSGGLIIA
                     DNVLWGGKVVDASENDEHTKALRAFNTMVQNDMGVENVLLPLRDGIMLIYKL"
     gene            complement(9856..11076)
                     /locus_tag="PK839_02375"
     CDS             complement(9856..11076)
                     /locus_tag="PK839_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008769163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOG71612.1"
                     /translation="MEFEQRTLHNGIQIVHKRVSSPVAHCCILINAGTRDELDHEHGI
                     AHFIEHVLFKGTKKRRAFHIMSRLEDVGGELNAYTTKEETVIHASFLKQDFNRAMELI
                     TDIAFHATFPEKEIAKEKEVVIDEINSYKDSPYELIFDDFEELIYPNHPFGRNILGTK
                     NHVSRFNRKGIAAFISRCYNTDQMVFCSVGDIPFTRVSRLADKYLNGISANKRSKPRL
                     ELGNYRPQDKIIKKSTFQTHCIIGTTAYDLQNPKRIAMHLLNNILGGPGMNSRLNLAL
                     REKHGFAYNVESSYTPYTDTGVFTIYFGTDKANVEKSHHEILKELARIREQRMGVMQL
                     FKAKRQLIGQTAIAADSNENLMLSAAKSFMVYQKPEDLTEVFNRIENISSSDILEVAN
                     EILSENRLSTLIYR"
     gene            11348..11420
                     /locus_tag="PK839_02380"
     tRNA            11348..11420
                     /locus_tag="PK839_02380"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11381..11383,aa:Phe,seq:gaa)
     gene            complement(11663..14515)
                     /gene="uvrA"
                     /locus_tag="PK839_02385"
     CDS             complement(11663..14515)
                     /gene="uvrA"
                     /locus_tag="PK839_02385"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007761222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOG71613.1"
                     /translation="MEFLKGEEEEVVVEGARVHNLKNIDVAIPRGKLTVITGLSGSGK
                     SSLAFDTIYAEGQRRYMETMSAYARQFLGTLERPDVDKISGLSPVISIEQKTTSHNPR
                     STVGTTTEVYDFLRLLYARASTAYSYNTGEEMVRYTDEQIISLIVEKFTGSRTLLLAP
                     LIRGRKGHYKELFEQLRHKGYLQVRVDDEIIEMRPNMKLDRYKTHHIELVVDKMVVEP
                     DNSKRIAQSVATAMNMGKGTIMVLNADDGSWRYFSRNLMCPTTGISYEEPEPYTFSFN
                     SPKGACPHCNGLGYISEADISRIIPDPSVSIKKGGILPLGPYKNSLIFWQIEAIASKY
                     GFSISDPISTIPEEALNVILYGSEEQFKLKHTGLGLTSSYFLSFEGVVSFVQNHSSND
                     ELNGKGDKWANQFIRQVTCPHCCGSRLKNESLWFKIDGKSIADVASMDIVSLSKWLQG
                     LEDRMNSRQRAIAAEILKEIRERVGFLLDVGLGYLSLSRGTVTLSGGESQRIRLATQI
                     GSKLVNVLYILDEPSIGLHQRDNVRLINSLKQLRDSGNSVIVVEHDEETILSADYVVD
                     IGPLAGRHGGRVMATGTPAEVMKTNSLTAQYLRGEREIAVPTKRRKGNGKIVRIRGAR
                     GNNLKNIDVEFPLGTMICVTGVSGSGKSTLVNETLQPALSQQFYNSYKQPLDFDGIDG
                     IEHIDKVIEVDQSPIGRTPRSNPATYTGVFTDIRKLFELTPDAKIRGFKAGRFSFNVK
                     GGRCETCKGAGVRVIEMNFLPDVYIKCPDCYGQRYNRETLEVKYKGKNISEVLDMTIN
                     QAVEFFGSIPQIHQKLKTLQEVGLGYITLGQSSTTLSGGEAQRVKLSTELAKRDTGKT
                     LYILDEPTTGLHFEDVRILLDVLNKLVDRGNTVIVIEHNMDVVKVADYIIDLGPEGGA
                     EGGKVVCTGTPEELIESACGYTATYLKRHLNREFNT"
     gene            complement(14537..15505)
                     /locus_tag="PK839_02390"
     CDS             complement(14537..15505)
                     /locus_tag="PK839_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HOG71614.1"
                     /translation="MFKNRKFLGAAIALLAVATCYVFIAFIPSAHSKPPIEKPDTVLQ
                     DGYIFPIPIPQNLSFAGENVPLDYFDVRESLDRELQVNTFWHSQTVMLLKRANRYFPV
                     IEPILQKNGIPDDFKYLAVAESGLMQVVSPSKAAGIWQILEGTGKDYGLEVNSEVDER
                     YHVEKSTEVACRYLNKSYRKYGSWTMAAASYNYGQNGMDKQIDRQQGDSYYNMVLGEE
                     TGRYVYRILAIKVIFENPRKYGFFLDSADLYPPFRYREVSVDTAISSISNFARRFDTN
                     YKVIKLFNPWLRESYLNNKAGKHYRIKIPDINFRVEAYAAGKKDEQ"
     gene            complement(15702..16733)
                     /locus_tag="PK839_02395"
     CDS             complement(15702..16733)
                     /locus_tag="PK839_02395"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HOG71615.1"
                     /translation="MTTHHIHIATLKRFGQLLLLLLLIISCQARQTATYTKVNGFTQG
                     TTFNIIYSDSAGRDFSKAFDSIFHAVDRSMSVYNDSSIISRLNRNEIDTVDALLAEVI
                     AISDSIYHETDGAFDITVGPVVRALGFGPEQHAGIDSAQIRALQRLIGMGKINMAGNR
                     LQKDDPKIQIDVNAVAQGYTSDLVARFLSSKEISNFMVEVGGEIMAMGISSRGTPWIV
                     GIDKPVENALPGENLQAKFYFSGSRGMSTSGNYRKFIEVNGIKYAHTINPKTGFPAMS
                     NLLSATVIAPTAARADALATAFMVNGLEWSRNFVNSHTGVEAYLIYSDSTGKFNEWVS
                     EGLKSSIAQ"
     gene            complement(16863..18026)
                     /locus_tag="PK839_02400"
     CDS             complement(16863..18026)
                     /locus_tag="PK839_02400"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008772393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018455 - alcohol dehydrogenase [NAD(P)+]
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOG71616.1"
                     /translation="MNNFEFFNPVRILFGNDKIEKIGMQLNPTDRILMTYGGGSIKKN
                     GTYERIKAALSNFSVFEFGGIEANPKYETLMKAVEVVKKNNVDFILAIGGGSVIDGTK
                     FIAAASLYEGSDPWDLLIKNIVVTKAIPFGVVLTLPATGSEMNSGAVISRLETREKYS
                     FGSPVLFPKFSVLDPTLTFSLPPHQVANGIADAFIHVMEQYMTFPSDSPIQDRFSESI
                     LQTLIEVGPQTLKNPSDYSLRANFMWSCTMALNGLLSTGVTTDWATHMIGHELTAFFG
                     IDHGATLAAVYPALLTELQEPKHEKIIQYGSRVWGLSGADLTARNVINKTEDFFRSLG
                     IKTRLCEYGLKQEDVAPIVERFRTRGWKLGERKNINHEVVEAILKRALEPYRS"
     gene            18338..18862
                     /locus_tag="PK839_02405"
     CDS             18338..18862
                     /locus_tag="PK839_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG71617.1"
                     /translation="MNKLTLGKYHILVVVAVLAATVVLLSCRQSKQENVKSSADKARE
                     NVVSWMESMKLEYPGYQPAEFGELTPRYNKSNITFHLYNLIEQERSKPSPSKRAIDSL
                     ENLLRDNPGELLGYSITHKYNVKDISGEIRQVESLFLLDTLLRVITVLDADAYDQIMD
                     EELFFRPESKDSTL"
     gene            complement(19029..19919)
                     /locus_tag="PK839_02410"
     CDS             complement(19029..19919)
                     /locus_tag="PK839_02410"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HOG71618.1"
                     /translation="MRKIAYLLTAGVVAVSATTLRAQQEPIFTHYFQNPVSINPAIAG
                     TVRNLNLSLLSRLQWVGLEGAPKTFSLSAHMPWPEKRMGIGLNIMNDDTWPVSNTHIS
                     ASYAYRLRLTDEITLSLGIKGGFTYYHASLTNLQINDPNDPEFTQNESRFYPNLGAGA
                     YIYSGEFYAGLSLPRFLQTSFNKKFDKTLKSPLYLMGGYNLEISPSWVFMPSMLMGAM
                     IGLPMSCDLTAQFLYQSKFYFGTHYRIGDALGIFFDMKVNEYLSFGYAFDFSLNKLSR
                     INTGTHELMLSYAFAPKWKF"
     gene            complement(19949..33520)
                     /locus_tag="PK839_02415"
     CDS             complement(19949..33520)
                     /locus_tag="PK839_02415"
                     /inference="COORDINATES: protein motif:HMM:NF037144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOG71619.1"
                     /translation="MRKLFTGLTIAFILQLFSGISHAEGTKQLMPNSSDRLWLEFNVF
                     GTGSSSFAGYNCSAEKRLNIRLVAGEKVFFGFKMNTINYGGNVYTNPNNAYYRIRKPD
                     GSIAVAESNIPTSGAGYIANYSQAIAGPNGAKLNGVTITGGYSPKTFTADQTGDYYIE
                     FRYYNNYRFALEFFDITVTDSNNNIITNPGEPNKPAGRIWSKTWQLTTTSFTQYPVNN
                     YFYVFTSDDFVNKINFQYYPFSFTFQSNSYGLITPTNEPNYIKRAQSKDNDQTSAASE
                     YRIYLNDPDRNIWPTTRLAAPKTQVWANDTLFFDYQYDRTPQQLAFADHDIYLTKNKV
                     GCEYSSIAIFKINSNIDAFTAILIDLDGDGGYSTAGNDRVIYRNMRRGNNYILWNFKN
                     DAGAEVATGVYHASATFMGRGPTNFPVYDVEQCDGVITSSIRPFRKLNATIYWDDTYI
                     SRWGDETGHGLMDETQRKQLKVSTIIPRIWSWNANLENTNFNGNLNTMNTWFNAIDLG
                     YSDITIHVNQSTTQCINGLAPWVGDIYKQGLPNNTITFTQADFTTKFFDPIEDPLTRI
                     RIKSLPANGSLKLSGTAVTVNQEITLANLANLTFTPNTGWVGITQFDWEAKNSQFWSM
                     NADKVYITVNTPPTISSIADQNLCTNNPSPAIPFTVNDSETPAANLTVTAYSHDPTFV
                     PNSSITIGGSGTNRTISVTPVANKSGHAIIYVMVDDGLSQTIQEFAVYVGPDLEFSGD
                     TTICVGQDLYLVAQEVGASSYTWKYNGTVVASTQTLSKAWGSFSAGSWSLTVSKGSCV
                     STRNFQVTIFPLTTFTGDQNVCVGEEINLSATENNASYEWKKGTTIVSSTKQFVKPSA
                     TLSDAGTNYTLKVTKSGCSATSNPFAITVINAPNVGLTVTGSTVDPGRNGTITIGSAQ
                     SGVTYNIYKNETLITSGVGAGANLTITVPAANLSVGSNLFVVKADNGNCEVPLSSTAT
                     ITVREPGFTITVISANTTEAGGTASFTVVCKTQPLANVILQFTSTDLTEGTVVSSITF
                     TTANWNTPQTVTVTGVDDYVVDGNISYTIQVGPSSSSDSYYNGIDPPDINMVNTDNDV
                     AAVVVNPTSGLTTTEAGGTATFTVRLAAQPGANVSLTLNSSNTAEGTVSPASLTFTSA
                     DWATPKTVTVTGVDDYVDDGDVTYFIQTSGTTSTDPLFNNLNVADVTVINTNNDVAGI
                     VVQPTSGLTTTEGGGQANFTIRLNSQPVSSVTVGLSSSNTGEGAVSPLSVTFNAGNWN
                     LSQTIIVTGVNDDVDDGNQSYTIVTAPATSTDTRYGGTNASDVSVVNIDDDDAGIIVS
                     PISGLITTEAGGSATFTVKLNSKPTANVTIALTSSKPAEGAVSPSTLTFTPSNWSTNQ
                     TVTITGVDDFVDDGNQSYTINGLASSSDGNYTGRSFTVSATNTDNDEAGITVAPTSGL
                     TTSESGGQATFTIVLNSQPTADVTIGLSSSNTAEGTVSPTSITFTSATWSIAQTISIT
                     GVDDMVADGPVSYTIVTATASSSDSKYSGLDPADVSVINNDNDVAGITVTPTVLTTSE
                     AGSSVTFTIVLNTKPSGDVTINLSSNDLTEGTVSPVSVTFTTTTWNTPKVATVTPVND
                     DEDDGDVAYTIVTSNATSSDPGYNNMNPADVTVTNVNDDVAGFTVSPTAGLQTTEAGG
                     TATFTVKLNSKPTANVTINLSSSNPAEGTVNPTSLTFTPTGWNTSQTITITGVNDDVD
                     DDNVAYTIITSVATSTDAKYSGLNPTDVSVTNIDNDTAGFTVSPTTGLQTTEAGGTAT
                     FTVKLNSKPTADVTISIASSNPAEGTINPTSLTFTPTGWNTPQTITITGVNDDVDDDN
                     VAYTIITNPATSTDTKYSGVNPTDVSVTNIDNDTAGYTVNPTSGLQTTETGGITTFTI
                     RLNTRPTANVTISITSSNTNEGTVIPTSVTFTPATWSNPQTITITGVDDDIDDDNVAY
                     TIITGAATSSDTKYSGLNPADVSVTNIDNDTAGFTVNPTSGLQTTEAGGTATFTVKLN
                     SKPTANVTITLASSNPAEGTVNPTSLTFTSGNWNSLQTATITGTDDDVDDDNVTYTIT
                     TGTATSTDAKYNGLNPPDVSATNIDNDDAGVAVSPYEGLYTTEAGGTATFTMKLTSKP
                     TANVSITLVSLNTNEGVVSPSTVPFTPDNWNTNQTITVTGMNDDVDDDNVAYTIQTQP
                     CISSDPKYDGLNPVDVSLTNIDNDNAGYILSQQNITYSESDSPVSVTYRLSSKPTANV
                     SFNLESQNTSKGTASPASLTFTSGNWNVEQVVTITPVNNYVVDGDATFAIATYSATSG
                     DSKYNGLNPPDVNVTCTDDDVAAINVSAISGNTREVGTTATFTIVLNSQPAADVTIGL
                     SSSNTAEGTVSPASVTFTSSTWNVAQTVTVTGVNDVVADGNITYTVIIGAATSADPNY
                     NGLNPDDIAVINIDDDQAGVNVFPVSGLTTKEDGSTATFQISLNTAPVSNVTISFASS
                     DETEGTVSPTQQVFNNTSWNSPATITITGVDDLAEDGDQPYTVTISSASSDPNYNNLS
                     IPSVSVTNLDDTKPRAADDAATTPEKDPVQVDVLANDKGLDKNGIVVTIAANPTHGSV
                     AVQPDNTITYTPNGLFNGDDTFTYRVTDRDGGYDEANVTITVTFVNDIPVAVDDARGT
                     SINTPRVVDVLFNDHGLEDGGISVTITEAPNPTQGNATANADNTVTFTPASGYLGVAT
                     FKYKVEDVNGDSDEATVTINVRPVNHIPDAVDDNATTFINTAQKINVLANDAGLDDGF
                     GSLIIFANPAHGIVVVNPDRTITYTPDAGFSGTDNFSYLIEDVDGDYDIAVVTITVTS
                     VPNHLPTAYDDYRGASRNTPVTVDVLINDTGLEDGLQSLTVTTQPVNGNALVNPDHTI
                     TYTPNTDFVGVEIFGYQVCDVDGDCSTANVHINVKEGINIVPVANDDTDSTIVNTPKS
                     INVLANDTGLDDGLGSITIFQQPEFGAVVVNANRTITYTPTYMFVGTETFRYMVTDVD
                     GDYDIATVTIKVKERPNYIPVANDDRRGCSFETPVVVDVLFNDTGLEDTPITVSISQQ
                     PTSGNAIVNPADNTVTYTPATGFIGEMTFRYTVADADGDSDDALVTIKVKSGVNMVPI
                     ANADNATTIMNNPVTINVLSNDTGLDDGFGGLYIYQQPEFGTVVLNANRSVTYTPAHM
                     FVGVETFRYYVEDADGDYSLATVTVTVIEKPNYVPIANDDYRGCSFNSSVTVDVLSND
                     TGIDDVPLTLSININPTDGNAQVNPDNTITFTPFDGFIGLTTFGYRVTDANGDSDEAM
                     VFINVKAGINAVPLARDDNAHTFINKSVEINVLANDEGLDDGFGALTIRQQPAFGTVV
                     VNANRTLTYFPGYMFVGSDSFSYMVEDVDGDYSVAIVTVVVDEKPDSKPVANDDSRGC
                     SFNSAVTVDVLINDSGLDDVPLAVSVTEAPQSGSAVPNPDNTITFTPEANFTGTITFR
                     YTVTDVDGDFDDAQVTIHVKAGQNIVPYAVPDNATTLVNKPAVVNVLANDTGLDDGFG
                     SLTVFEQPKFGSVVVNSNRTVTYTPSYMFIGVETFRYMVTDVDGDYAIGTVTITVIEK
                     PNSIPVANDDRRGCSFETPVVVDVLVNDTGLEDVPLTLTISELPTIGSTVVNPNNTIT
                     FTPPAAFTGIATFRYTVTDSDGDSDDALVTIRVKEGENYVPHSNDDAASTIINTNVDI
                     NVLANDTGLDDGFERLIIFEMPSFGTVTVNENRTVTYTPSYMFLGTDTFRYWVEDVDG
                     DYSIATVTVEVTERPNYLPVANDDYRATSLNTPRIVDVLVNDTGLDDTPVEVSITNQA
                     SHGTTVVEADNTVTYTPQNSYLGADHFSYRVTDRDGDWDEATVFIDVKPVNMIPVAVD
                     DYVQTYVNQPVDIHVLENDSLLYEGIKSLQVFSAPKWGTVQVNDNHTITYTPSYFFIG
                     NDEFKYLVEDVDGDYDIATVYVVVLATQNAIPVANDDHRATSKNTPLDINVLINDDGL
                     DDGWIRVFIDEFPDAATGTAAVKPDNMVSFIPATDYLGLSTLKYFIVDRNNDTSNVAT
                     VTVNVKEVNFIPVANADTASTNMNVPLLVDVVNNDTGLDDGLDYIKVMGKPLHGFAYA
                     FDNHNIKYFPSSWFVGTDSLTYLVADEDGDYDIGKLYITVNERPDHKPLANPDGRGTT
                     TNTPVTVDVLFNDTGLEDGGIKVLLNIFPINGNVLLNADNTVTYTPNTGFLGEDVFYY
                     QVCDFDSDCSSAAVTINVKNANLVPIAYNDTVTTFKNKAISINVLFNDRNLNDGGIRT
                     VVFSNPTNGAVSVNPDNSILYTPANNFFGVDSLWYYVADVDGDYSLAKVVIDVLNREN
                     FTPQANDDDVEIFMDTETTIDVLTNDIGLNDGVKALAVETSPTNGICRITSDFKVYYS
                     PLTGFVGTETFTYRVTDRDDESSVAYITVRVVPDPDGKVVVPEAFSPNGDGFNDTFEV
                     LNIGQFQHITLKVYNRWGNLVYKSDRYKNDWDGTANVSLSLGSKLPAGTYYYLLEILD
                     TGKTYKGSVFIKR"
     gene            complement(33638..34522)
                     /locus_tag="PK839_02420"
     CDS             complement(33638..34522)
                     /locus_tag="PK839_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG71620.1"
                     /translation="MEKFKMYFVKLILPIALTTLSLGSCIENQDLMTSNVRTGGILDA
                     SELVQFKPSAVNQDDSIYLSVYQGPPVVAVKVFKRYVHMADNSATPEMLLDNIVLNGA
                     NGTDTFYVTKIYSWDELKQGYSKLGNGIDDFPPDAASADVGDYFALKYVSVMDNGFES
                     LNRSTTKILVANSYAGFYTSHITYFHPTLGGAYPDKPYINTYTQKQLITLSVTSCTTN
                     FALWGSYGESMDITINPDNSVVFNIVGFDYIVNEGDPYNPAYHSYYDPTNSTIHLYYY
                     WEGVGGYRVIWETLSPNI"
     gene            complement(34556..36115)
                     /locus_tag="PK839_02425"
     CDS             complement(34556..36115)
                     /locus_tag="PK839_02425"
                     /inference="COORDINATES: protein motif:HMM:NF024181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HOG71621.1"
                     /translation="MDTPMWRWFKIYRWIAITGILVGMVSCTGDFEELNRNPNAIGAN
                     ADHPDMLLTHAVESLGDQLFDVWLGHEIGSCWVQHMAKVQYTDEDRYVPRNDVINSVW
                     NNLYAGPGIEIQRIYEIGQRKGHANYIGVALVLKSYLFSVLTDLYGDIPYTQAFNPDS
                     TLTPQYDTQESIYLNLIQNLKYANELLNPYGDGIEGDILFHGDILKWKKFANSLRLRI
                     LLRMSGHAEQEQFVKSEMSSILGNSTDNPIFTSNSDNAALHYLGSYPNNHPLNETYKT
                     RDDHRVSRTLVDVMYNPGNHDNPDWRIVVYAQTPQAGGFWIGLPNGLTSAKAAAYLGN
                     GIRRTSKIGEYFIRPDAPAMFMSFAEVNFILAEAAQRNLIDISPLTPKDYYEMGIDAS
                     YRQYAREIVENIPVYYPSFEGVSINIDDFITDFLNHNGAWDAANPLQKIATQKWVAMF
                     DQGLQAWFEWRRTGYPVLTPAEDGLNGGKIPVRLRYPSDEYSRNGENVAKAVALQGDD
                     NLNTPVWWDCN"
     gene            complement(36128..39247)
                     /locus_tag="PK839_02430"
     CDS             complement(36128..39247)
                     /locus_tag="PK839_02430"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOG71622.1"
                     /translation="MRKLSGFLSFFFIILLVLPASKIAAQNIKISGTVISLTGGYPIR
                     GVTVVVVGSARGTFTDSLGRYELVTPSISKTLRFTCIGYKTQEVDIAGRRVIDVALEE
                     EAIEMQEVKVTALGIMRAERSTGYALQSVKGEDINRTSETSLLNALQGKVAGAIITST
                     SGAVGASSRIVLRGINSLNTDNQPLFVVDNVVLSNANFGNTTTEGVNRGSGVADINPN
                     DIEDITVLKGPNAAALYGSRAANGVIIIKTKSADVDDHFGVSFNSTITMESPMRLPEF
                     QNKYGQGSGGLFEFYDGMGNGINDNVDESWGPPLDIGLMIPQWNSPVAGGIRQPTPWI
                     SHPDNVKNFFETGMTFTNNVSVEGADESYAARFSYTNTEQKGMVPNTDQSKNNVMLNA
                     SISPYTYLTLNASLGYVNTHSDNLPAYGYDAQNVMQQFLWFGRQVDVADLRQYRFPDG
                     SKRNWNYSYHNNPYFTLYENLNGLDRDRLFGQGSINLKFTDWLNLKVGAGIDYFKNYN
                     NARSAFGDIDSPLGYYGESHLTFRELNTDFLLSFNRNLSSKLSLSFNLGGNLMRQYSQ
                     SLNSYATELSVEDVYTLENSRGPVVSINSLSRKQINSLFYNGQLVWKNALFFDFTGRN
                     DWSSTLPLNNNTYFYPSFNLSAVLSDLFDFQSPTLNMVQVRAGWAQVGTDTDPYQLQP
                     VYRFDQGWHESTKLPAIYIPNDLPNPNLKPQRTNSLEVGATLKLFMSRLGIDLTYYNQ
                     KTFDQIIRLPVSATTGYTSKIVNAGEIDNSGIELTLDGVPVRTRSGFEWSINLNFAWN
                     KNEVKELAEGLKQYVLGGYWSLDVLAIPGQPVGTLYGYDFKRNENGKIIFYDGLPAQG
                     EIHALGNYTPDWTGSIANSLRYKNLSLNFLIDARFGGEIYSMTSTWGRYAGVLKETLK
                     GREGGIIGVGVMQNEAGEWVPNNVVVTAEEFNKAAYNNNIAYSSVFDASFVKLREISL
                     GYTFNHIANNLIKEISIAVVGRNLALLYARVPHIDPETAFSNSNVQGLEFGQLPSPRS
                     YGLSLNLKF"
     gene            39672..40166
                     /locus_tag="PK839_02435"
     CDS             39672..40166
                     /locus_tag="PK839_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008149876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOG71623.1"
                     /translation="MKRIGILSDTHGTLDDGVMEFFSDVDEIWHAGDIGSLSLADRLA
                     SFRPFRAVYGNVDDARVRLSYPLSQHFRVEDIVVFMTHIGGRPGRYEPIVYEAINSNE
                     VNLLVCGHSHILLVKFDSKHNLLFINPGAAGNNGFHKVKTVVKLDIEGKQMKNLQVWE
                     KTRF"
     assembly_gap    40318..40417
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(40481..41752)
                     /locus_tag="PK839_02440"
     CDS             complement(40481..41752)
                     /locus_tag="PK839_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOG71624.1"
                     /translation="MGTTIIIILISLFFSFFFAGMEIAYLSANKLKLELDKKKGGFTS
                     HVIAVLAGNKEQYIATMLVGNNIALVIYGIEIAVLLTPALGKLIQIPSLLLLVQTLIS
                     TIIVLVTAEFLPKSIFRLHPNAFLRFFALPVYILYIIFYPISKFTSWLSYWIIRLVFR
                     IKVKHDGSKPIFNKVDLDFLVDQASSADDGEGDHTQELKIFQNALDFSEVRLRDCMIP
                     RTDIEAIDVNATVDELRAKFAETNYSRLPVYSNSIDNIVGYVNSKVLFINPQSIKSKL
                     INIDFVPETMPANKMLTRFIKEHKSIAIVVDEFGGTAGLVTIEDIMEEIFGEIDDEHD
                     RNEFVEKKLSDNEYIFSGRLEVKHLNENYQLDIPESDDYETLAGFILFKHQNIPKLNE
                     TIEFEGFIIKILRVNETRIELVRLTRIKGKG"
     gene            complement(42288..42854)
                     /gene="lptC"
                     /locus_tag="PK839_02445"
     CDS             complement(42288..42854)
                     /gene="lptC"
                     /locus_tag="PK839_02445"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HOG71625.1"
                     /translation="MRITLFKGYRYILPGIAIVGTTFFFGCKTDTEAIQYFSDKKSIP
                     GVTAYNSELEYTENGVVKIMVKAPITIYYQFSEEPYTEFPEGITVYTYDDTLGVESNL
                     TAKYAIYYEKKMLWHAKNNVVARNRKGEVLNTEELYWDQGKHLIYTDVNVKINSVNGN
                     IYGKGLVSDEKFDSWEVKEPYDGTIQLE"
     gene            complement(42873..44171)
                     /locus_tag="PK839_02450"
     CDS             complement(42873..44171)
                     /locus_tag="PK839_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG71626.1"
                     /translation="MQNSRKILLLVGFTLAALEVFSQNIYTYSPYSRFGIGIIPTHGF
                     SQTRAMGGVSQSVRSPYMINYLNPASYAAQDSMSFTFDFGLESNNSSFSGYDQYLNKV
                     SASSSAGGIHHIAIAFPVSKKIGMAAGIAPYSSVGYRLIRFETDPYILSTIGRIKYEH
                     AGKGGINQLFVGAGYAPFKNFSIGANFIYYFGSLDYNNNIIFPSSSYSYTNASVGSSI
                     MVSDVSFNVGFQYRLFLDEDKNNSLGFGVTVDAGSDINMKYKWLTKLEGSTYVDSLIP
                     HPQSKSKFKLPTGVNSGISYTFKDKLFATAEYYYQDWTKTEAFNTTLQMGKMEAFRLG
                     VEFTPNRSDLKSYLKKISYRAGGFYNKTNLIINSRQIEEYGISLGFGLPFRNNTSFNL
                     SAELGRRGTNENYLIKESFFVLNLGITFHDSPWFFKRKYY"
     gene            complement(44161..44883)
                     /locus_tag="PK839_02455"
     CDS             complement(44161..44883)
                     /locus_tag="PK839_02455"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HOG71627.1"
                     /translation="MNLIIDVGNTQVKVALMEQGEIVFLKSFSTLNAGSMEEILSTYL
                     PHKAIVSVVGNFQPEILSYLSQKLMVHVLGSKSKLPLKNTYETPLTLGYDRIAVAVGA
                     ITLYPNQNVLVVDCGTAITYDVVTSDEIYLGGGISPGISLRFKSLNDYTAKLPLLSIS
                     NTFSLIGRNTNDCILSGVLNGVVAEVDAYIEKFNLIYSNSRVVLTGGDSSFFVDKLKN
                     SIFVNPNLIYTGLNRILELNAE"
     gene            complement(44924..48268)
                     /gene="mfd"
                     /locus_tag="PK839_02460"
     CDS             complement(44924..48268)
                     /gene="mfd"
                     /locus_tag="PK839_02460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HOG71628.1"
                     /translation="MQHQHELYKLYLKHPSLKQLLDAGQNLEGGFFLKGLAGSAVAFV
                     LSGYVEQRRRPSLVVVNDKEEAAYLFSDLSCLLPENQVYFFPSSYKRSVQYGQTLPDA
                     IVQRTDVVALLPALNENDERPVVVVTYPDALFEKVVSLDTLSRNSIKIQKGEKVSPDF
                     ICDTLTEYGFQRSDFVYEPGQFSVRGSIVDVFSYSASTPYRLDFFGDEVETIRSFDVE
                     DQLSKELLSEVVIIPNLQSPEDNPSRILLTDLLPPSTTLWVKNPELVLNRMDELSRIV
                     STATQLNASNFASRDIFSNSLLTRSTLYFGIKPLSVQLPTFDFGTSPQPAFGKNFELL
                     VQKINEDAGRGYQTFILSDNSAQLERLDGIMHGIAPDIAYNPVEGTISEGFVDHNLRI
                     NLYTDHQIFERYHRYKLKHEFNRRDAITMQELVDLQPGDYVVHIDHGIGIFGGLVSTE
                     INGKKQEAIRLVYQDNDTLLVSIHNLHKISKYRGKDAESPKIYKLGSGAWQRLKQNTK
                     RKIKDIARELIALYSQRRSQPGFSFSPDTYMQQELEASFVFEDTPDQLSATKAVKEDM
                     EAERPMDRLICGDVGFGKTEVAIRAAFKAVADSKQVAVLVPTTILALQHYQTFTSRLK
                     GFPCTVEFLNRMKTPAQQREIRQRASEGKIDILIGTHALLNSTLKFKDLGLLVVDEEQ
                     KFGVAAKEKLKQLKLNVDTLTLTATPIPRTLQFSLMGARDLSIINTPPANRHPILTEV
                     HTFSTAIIKEAIDYEMDRGGQVFFVHNRIQNIAEVELMVNKICPKAKTAVAHGQMEPS
                     KLEKIMLGFISGDYDVLISTSIIESGLDISNANTMIINNAQMFGLSDLHQLRGRVGRS
                     NKKAFCYLLAPPLETLTADARRRLRAIEEFSELGSGFSIAMQDLDIRGAGNILGAEQS
                     GFITEIGFEAYQRILDEALEELRESEFVTIQTQAEHETQREWKPSYADCHVETDMEIL
                     LPDSYITNTTERIKLYREIDSLHNAEELERFRNEIRDRFGPLPRQVEDLLSVVRLRWI
                     AVELGFEKLILGNGQLMAHFISNQLSPYYRSSTFEGIIRFIQQHPERFKLKEGKDKLT
                     IRIAGVGEISEAIETLQKLHETLPVK"
     gene            48502..49275
                     /locus_tag="PK839_02465"
     CDS             48502..49275
                     /locus_tag="PK839_02465"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HOG71629.1"
                     /translation="MEKGVVSKSMAALRREPTHTSEMVNQLLFGETFDMVERQGGWLK
                     VKGHTDGYSGWMDERLSLQLPDNSPIFTDKPKIASRLFRAKAASGFTTYLCPGSQLHS
                     FDGKTFRNGSSSFVCMDTPFAKLPDNPREKILALSTTFMDAPYLWGGRSLFGVDCSGL
                     VQVVFEIAGTMLPRDARDQAGIGQTIEFANEAKPGDLAFFDDENGVITHVGIVAENAT
                     IIHCSGFVRVDRFDHQGIFNLEAHRYTHKLRIIRRVIFD"
     gene            49317..49445
                     /locus_tag="PK839_02470"
     CDS             49317..49445
                     /locus_tag="PK839_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG71630.1"
                     /translation="MLKNQIVIVFVIILIAVWIAFKIRNKRRNQGDDDENNDELSM"
     gene            49457..49894
                     /locus_tag="PK839_02475"
     CDS             49457..49894
                     /locus_tag="PK839_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004343945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOG71631.1"
                     /translation="MYCYEYPRMLVTVDAVIFRTDTQGHPVDLLLIERKNEPYKGCYA
                     LPGGFPEMNELLSDAAARELKEETGLDGITLKQVGAFDRVDRDPRDRNISVAYLGFAS
                     ADSPLPNAGDDAATASWFPLNNLPPLAFDHAEILEKALSNIGI"
     gene            complement(49895..51172)
                     /locus_tag="PK839_02480"
     CDS             complement(49895..51172)
                     /locus_tag="PK839_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOG71632.1"
                     /translation="MEQEITSSAYLDMELLRFTTAGSVDDGKSTLIGRLLYDSKAIFQ
                     DQLEAIERASIKKGEDSLDLALLTDGLRAEREQGITIDVAYRYFHTPKRKFIIADTPG
                     HTQYTRNMVTGASTANAAVILVDARHGIVEQTIRHAYIASLLRIPHVIVCVNKMDMVD
                     YAQESFEAIQRRFLEFSSFLDLYDLRFIPISALLGDNVVERSKHMDWYQGPTLMYLLE
                     NLYISNDYNQLDARFPVQYVIRPQSAEFPDYRGYAGRMASGTFRVGDRVKVLPSGFSS
                     VIKGIDFWTDSLDSITAPQSGTILLDDDLDISRGGLIIPEENGIKQGQEVDLMICWFS
                     EKPLVVGGKYAVKHTTNDLRCLIKEVKYKVNINTLEKDYHEKSIGMNDIACITIKTTK
                     PLFYDSYRQNRITGSLIIIDEATNVTVGAGMIV"
     gene            complement(51285..52190)
                     /gene="cysD"
                     /locus_tag="PK839_02485"
     CDS             complement(51285..52190)
                     /gene="cysD"
                     /locus_tag="PK839_02485"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HOG71633.1"
                     /translation="MSKFKLNHLRELESESIYVIREVVSQFENPVMLFSGGKDSIVMF
                     HLARKAFYPAKVPFPLLHIDTGHNFPETLEFRDWLVKETGVQLIVRYVQDSIDRGRVA
                     EEKGYNASRNKLQTVTLLDAIEELKVDCAMGGGRRDEEKARAKERFFSHRDEFGQWDP
                     KNQRPELWNLFNGRKNLGEHFRVFPISNWTEMDVWQYVLMEQIALPSLYFSHERDVFM
                     RDGVLYAKTPFLPMKDDEVAEKRIVRFRTIGDATCTGAVESAASTLEEIIREVAATRT
                     TERGGRADDKRSEAAMEDRKIEGYF"
     gene            complement(52305..52847)
                     /gene="cysC"
                     /locus_tag="PK839_02490"
     CDS             complement(52305..52847)
                     /gene="cysC"
                     /locus_tag="PK839_02490"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HOG71634.1"
                     /translation="MTSCTPATIWLTGLPSSGKSTIGLGLGRMLDGIGVGYVLLDGDE
                     VRSGVCSDLGFSDDDRKENIRRVAHIAKLLNSQGLVAICCLVSPLQSMRDLARSIVGS
                     RFFYEVFVNASLETCMKRDVKGLYAKAEQGQLNNLTGFSAAYENPTDPNLVIFNNSIG
                     VEEAVNMLFTHVVDWLEIPW"
     gene            complement(52897..53541)
                     /locus_tag="PK839_02495"
     CDS             complement(52897..53541)
                     /locus_tag="PK839_02495"
                     /inference="COORDINATES: protein motif:HMM:NF027898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HOG71635.1"
                     /translation="MRSLMLFVFALLSVNLVAQESQDVPQTFWDFSSKILEASSINAT
                     FSLTIENRQENVANTQQGEILIKGNKYLLKIMGMEVYFDGSTKWQYIGEANEVTISTS
                     GGELGSPLDDPAGIFRDYESRFKVRFISETTVKNRTLVEMDFYPKALDSPYVSVKLRF
                     DKVSLEPVFIRYHGKDGTSYIVNISQFKTNVPAPDGTFTFDISTRKGIEVIDLR"
     gene            complement(53604..56066)
                     /locus_tag="PK839_02500"
     CDS             complement(53604..56066)
                     /locus_tag="PK839_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009129003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HOG71636.1"
                     /translation="MASDSNRPNVKEKNNATKEGQDNGKNKLLRDERVRFTIGLFLLL
                     LGVYFLISLISYLFTWKVDQSLEWQSLFSKAEYEVKNWTGRFGAVISAQFMNHWFGLS
                     SLALPSIIIVFGLKLLKLYRKNIWRFIFNIFIGTLLCSLVLGFLFETAKGFLGSGLGG
                     AQGLFVSKWLSALLGKVGTSFLLLLLVIAYTIFIKPSTLHWFRRTIRGLLIRMRRPVS
                     RKNDKRQNKKEKTAEELAGQVDSEVLPNNTDAAVEVTLEKFIPEPNSELVDAEPFELI
                     EEETAGTNSSNAENTPEKSDVEFTIEKINEVELNPVEVNGQELYDPTLELSRYQRPLL
                     ELLEDYKNTTSPVSNETLIENKNRIVETLSHYKIQIDKIKATIGPTVTLYEIVPAPGI
                     RISKIKSLEDDIALSLAALGIRIIAPIPGKGTIGIEVPNQNPEIVSMHSIIKSQKFQE
                     CKYELAIALGKTISNEIFMLDLAKLPHLLVAGATGQGKSVGLNAIITSLLYKKHPAEI
                     KFVLVDPKKVELTLYSKLERHFLAELPGSEEPIITDTQKVISTLNSLCIEMDNRYELL
                     KLAQVRNIKEYNEKFVERRLNPNKGHRFLPYIILIIDEFADLIMTAGREVETPLARLA
                     QLARAIGIHLIIATQRPSTNIITGVIKANFPARIAFRVPSMIDSRTILDHPGANNLVG
                     RGDMLVSAGSDMVRVQCAFIDIPEIERITDFIGNQQGYPEAYQLPDYAPEGQDGFREI
                     DLNKRDELFEEAARMVVQTQIGSTSLIQRKLSIGYNRAGRIVDQLEAAGIVGPFEGSK
                     ARQVLIADELSLEQILRGLSNQ"
     gene            complement(56296..56988)
                     /locus_tag="PK839_02505"
     CDS             complement(56296..56988)
                     /locus_tag="PK839_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG71637.1"
                     /translation="MRKPIAGLVILSAFILCSCGGRVKNNSISEGVITYDITYDSAIQ
                     SRYDAWLLPSELIVRFNSNHSINSIEALGGAISISIIKNPERNEFSTLLKIFNKKLYH
                     SEPIEQERYPALYSRMPAVKLISEGETCEFLGYSCTKAKGYFADNPDFPFEIIYTSQI
                     QIENPNAHTPFEEVKGTLLQFNIRLNNLTMHATARSVKSEHVPLELFAPPSDYVVTDS
                     QTIANLIYTLQQ"
     gene            57310..58269
                     /gene="arcC"
                     /locus_tag="PK839_02510"
     CDS             57310..58269
                     /gene="arcC"
                     /locus_tag="PK839_02510"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HOG71638.1"
                     /translation="MSKLAVVALGGNALLRGNQVGTIEEQEQNTTETLENLVYLIKEG
                     YNLVISHGNGPQVGNILMRNDAGEQLYGIPQMPLDVCVADSQGGIGYMIERMLRNVLK
                     NHGIDKEVCCLVTPVIVDKNDPAFSNPTKRVGRIYDKAQADALASQKGWIFKEEVKDT
                     GSGWRRVVPSPKPKGILNIKVIDQLVDQGIIVIASGGGGVPVYIDEEKNVRPAEAVID
                     KDSASALMAAEIKADEFYILTDVPFVFINYKKPNEKKLEFLNHADATRYLQEGMFGEG
                     NMAPKIRACLNFIENGGKMSVITEATKLEDMSYGTKITMAYED"
     gene            58431..59432
                     /gene="argF"
                     /locus_tag="PK839_02515"
     CDS             58431..59432
                     /gene="argF"
                     /locus_tag="PK839_02515"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005318430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HOG71639.1"
                     /translation="MSFNLRNRSFLKLLDFTPKEIQFLLDLSADLKKAKYAGTEQQQL
                     KGKNIVILFEKDSTRTRCAFEVAAYDQGAHVTYLGPSGSQMGKKESMKDTARVLGRMY
                     DGIEYRGYSQEIVETLAKYAGVPVWNGLTTEYHPTQILADFLTMREHSSKPLSKVTFC
                     YMGDARNNMGNSLMVGAAKMGMDFRACAPKACWPKEELVKTCMEIAKTTGARITLTEK
                     VEEGVKGCDFLYTDVWVSMGEADSVWDERIKLLKPYQVNRKAMELTGNPNCKFMHCLP
                     SFHNRETAVGEDIFKKFGLESMEVTDEVFESDASIVFDEAENRVHTIKAVMVATLGS"
     gene            59542..59817
                     /locus_tag="PK839_02520"
     CDS             59542..59817
                     /locus_tag="PK839_02520"
                     /inference="COORDINATES: protein motif:HMM:NF012305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HOG71640.1"
                     /translation="MMIYVGNIAYSMTPDDLKELFAPFGNVLSVKIIADKTSGRSKGY
                     GFVEMEKDEDGQKAISALNDTQVKGRNIKVNNAFRKDDSKTEGKQKD"
     gene            complement(59961..60449)
                     /locus_tag="PK839_02525"
     CDS             complement(59961..60449)
                     /locus_tag="PK839_02525"
                     /inference="COORDINATES: protein motif:HMM:NF016213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HOG71641.1"
                     /translation="MVVDYVLIVLAAALVVVGLVGCFLPVIPGPPLSWVGLLLTYFTN
                     FVNFDWSLLLWTGIATLVVVILDYVMPVWVTRKMGGTKRGVWGSAIGLVVGLFVFPPL
                     GIIVGPFVGAFIGELTARDKSNNPLLSATGSFIGFLVGTGLKFAVSGYIAYRFTVEVF
                     FR"
     gene            60563..60835
                     /locus_tag="PK839_02530"
     CDS             60563..60835
                     /locus_tag="PK839_02530"
                     /inference="COORDINATES: protein motif:HMM:NF012911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HOG71642.1"
                     /translation="MTAKTKAISVKGRVQGVGFRYFVLRNAKRLGIRGFVKNMPDRSV
                     YIEAQGQEENLNELIGLCWKGPERSRVDSVETNEVVRKEWNDFYVE"
     gene            complement(61032..62042)
                     /locus_tag="PK839_02535"
     CDS             complement(61032..62042)
                     /locus_tag="PK839_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HOG71643.1"
                     /translation="MSDLKTSYMGIELKNPLIAGASSLSLNIESLKAIEAAGAGAVVF
                     KSLFEEQIQLEALELENELEEYNERHAEMVKLFPTLKHAGPADHLAKLRKAKDTLSIP
                     VIASLNAMYPDTWVEYAKEIEQTGVDGIELNFYTTPKNFEVEGKTIIQSQVDTLKSVL
                     EKVTIPVSVKLSPFYTNPLDVIRRFDETGIQGFVLFNRFFEPDIDIEKEELVQTITLS
                     NEDDLRLPLRFTGLLYGNIVADICSSTGIMTGADAVKMLLAGATTFQVTSTIYANGIE
                     QITKILGEIEAWMHRKGYKSLHDFRGKLAKKNLHDPFAYLRAQYVDILLRSNDIIKDS
                     AI"
     gene            complement(62054..63121)
                     /locus_tag="PK839_02540"
     CDS             complement(62054..63121)
                     /locus_tag="PK839_02540"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HOG71644.1"
                     /translation="MKPLNRIFIFLLLLLSVAKPSSAGRVVPNSINRSSIREIEASIR
                     NGDYGKVTSIVVLNRQGAILYEKYFGFSTRNSLHTISSVTKSITSLMVGICLDKGFIS
                     SIDSPIYSYFPEYKEVFEKDSTKKLITLRHLLQQTAGLNFKEWQFPYNYASNSLMATI
                     EENRSWVERFFYLPVDTLPGVKFSYNSLASQVIAEVLSRASGIAYDEMVREFLFKPLS
                     IENFLWESYAGNSLPAWGGLSLTTFDMAKIGLLVINGGIYGGRRLVSSEWVESSTAGR
                     VRVNDNIYYGLHWWVDILPSGKPLPFAAGYGDQYVFTAPDRGLVVALNAQNFSDFRFS
                     KSAIDLANILLSSVEVENFQH"
     gene            complement(63304..63597)
                     /locus_tag="PK839_02545"
     CDS             complement(63304..63597)
                     /locus_tag="PK839_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG71645.1"
                     /translation="MASRKDLKKDVDSLVGEVVNDCFTYMTLHGDKNRDKAEGIISKI
                     MESRNTAIKKINNPGKLTDKKATKAHFKALQQELLVSADECFTELSKLPKDLG"
     gene            complement(63739..>64053)
                     /locus_tag="PK839_02550"
     CDS             complement(63739..>64053)
                     /locus_tag="PK839_02550"
                     /inference="COORDINATES: protein motif:HMM:NF037202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG71646.1"
                     /translation="PISKYQGESENDRIEGIAYDEQKGRIYINKNKYFDGITPEVWGY
                     EIGGYQVLQKYLKDRKGRNMDDSKHYSRVITAISATIELQRQIDETYPLVENELVEFL
                     SA"
BASE COUNT        16651 a        14397 c        15165 g        17740 t
ORIGIN      
        1 acaggtgtta tagtgagtag ttcgactggc tcactataac accagtcgca ctaatcactg
       61 tagcaacttt tgaagggtga atgcctcaag ccatggttca tttaagctat ttttagctat
      121 tcaggccaaa ttgccaatgc caaaacatcc acctgttttt cacggccaat gaaaattgtt
      181 ttaactttgc tggctaaact aatattgtat taatggcagg aaaaaaagga aatgctgacg
      241 aaacgctaca gctgcttaaa tgcattgttg atgccattga ggaaaaaaaa ggattggatg
      301 ttgtttcgat tgaaattggc gagcttcaaa acgctgtttg cgactacttt gtaatttgcc
      361 acgcaaactc caccacgcag gttgatgcta ttgccgggaa tattgaattt aaaactttgg
      421 aacttcaaaa ccagcgggtt taccgttcat caggatatga aaatgcaatc tggattgtgc
      481 tcgactacat agatatagtg gttcatgttt tcctttcgga gtggaggaat tactataaaa
      541 ttgaggagct ttgggccgac ggtaaactat gccgctacag tgaccccgat gacgattttt
      601 ccagcatgca accagctact ttttaaactt ttggttactt taatggttat cttaccttca
      661 aaccgtaata aatgaattat gagcaacaat aaaaatagtg ggaatcaaat gccgggcgat
      721 agaaaagggc aagtgcctaa accgccaagg tttaatgttt actggattta cattcttatt
      781 attcttggcg ttttcttaat acaattcttt ttctcgggaa aaagtcctgt tgttacctcc
      841 tggcaggagg ttaagaacca gatgttggcc aacgacgagg ttagcaagct tgttgttgtt
      901 cgcaacatgg ggaaagttga ggtgtacctt aaggaggaca aacttaccaa gtacaaggac
      961 aggctgggag aaggaatggg ggctgttcca aaatcaggtc ctcatttcta cttcaccatt
     1021 ggctcggttg aaacatttga gaagcagctt aacgaagctc aacagggaaa acccgatgcg
     1081 caaattatct acccggaata taccgatcag cataactact ggggcgatat tctctcatgg
     1141 gtcattccaa tcctgctaat tgtgggtctt tgggctttct ttttccgtag aatgaccaag
     1201 aatgcacccg gaggtgcagc cggccccgga aacatttttt cggtaggcaa gtcgaaagcc
     1261 caacttttcg acaaggaatc gaaccaaaag attgatttta aggatgtagc aggtcttgaa
     1321 gaggccaagg ttgaggtgat ggaaattgtg gattttctga aaaatccaaa gaagtacacc
     1381 gagttgggcg gcaagatacc caagggagtc ctgctggttg gccctcctgg aaccggtaaa
     1441 actctccttg ccaaagcggt agccggcgag gctaatgtcc cctttttcag catgtcgggt
     1501 tccgattttg tggagatgtt tgttggggtg ggcgcttcaa gggtgcgcga cctcttcaag
     1561 caggccaagg aaaaatcacc ctgtatcatc tttattgatg aaattgatgc cattgggcgt
     1621 gcacgtggcc gaaatgccaa cttttcgggc aacgacgagc gcgagaatac cctcaaccag
     1681 ctgctaaccg agatggatgg ttttgcctct aaccagggtg taatcatcat tgcggccacc
     1741 aatcgcgccg acgtgctgga caaagcttta ctcagagctg gacggtttga ccgccaaatt
     1801 cacgttgagc tgcccgacta caaggagcgc ctcgatattt tcaaggtaca cctccgtccc
     1861 ctcaagctag agggcaacgt ggatatcacc tttttggcca agcagacacc cggattttca
     1921 ggggcagata ttgccaacgt atgtaacgaa gcggccctga ttgctgcccg aaaaaacaaa
     1981 aagcaggtgg agcggcagga tttcctcgat gctatcgacc gtataattgg tgggttggag
     2041 aaaaagaata agattatttc taaggatgaa aagcgcgcca ttgcctacca tgaggctggt
     2101 catgccaccg ttagctggct gctggagtat gccaacccgc tggtcaaggt ttccatcata
     2161 ccccgtggta tttcgctggg ggctgcatgg tacttgcccg aggaacgaca aattactacc
     2221 accgagcagc tgctggatga aatgtgcgca acgctgggcg gaagagctgc agaggagctt
     2281 gtttttggga aaatctctac cggagcgctc aacgaccttg agcgtgttac caagcaggcc
     2341 tacgccatga ttacctactt cggtatgggt gataacttcc gtaatatcag ctactacgac
     2401 tctaccggtc agtccgaatt cacctttagt aaaccctaca gcgaaaaaac cgccgagcaa
     2461 attgatgagg aggtgaaggc gataattgat aaagcctaca acagggcaaa ggaaatactt
     2521 agggagaata gcgatgggca cgtgaagctt gccgagctgc ttcttgaacg tgaggtgata
     2581 ttcagcgaag atttggaaaa aatttttggc ccccgtaaaa gcctctcccg cgaggaggag
     2641 ctggcaaaag aatttagcga tgagctaaag aaaacccaat cgccaaacga gagcgataat
     2701 ggatgatagc tggataaagg ttttttcaac ttctctaccc tggcaagccg aaatggccaa
     2761 gcaggtgctt gaggagaatg gaattgaagc ggttgttata aaccagcagg attccagctt
     2821 tttttttggt gaggccatgg tttacgttga gggaaaatgg gaggatcaag caaaagaact
     2881 gttaaaaggt attggtagtt gactgaattc ttaaaacgca ccatcagcgg gttaatcttt
     2941 gttgttacta ttaccggagc attggtaata ggtcatgttg ctttttttat tgttttcctc
     3001 gggctaatgg tagctactca gtacgaattc tataaaatga ctttcaaaac ccatctgcgg
     3061 cctcagatac ttcttggact ttttataggc atcgccctgt tttcatggtc atttctgtac
     3121 tccctcggaa aggttgaaca aattaccatt tttggtttca ttccgctact gggcggcatc
     3181 tttgtagtgg agctctaccg taaccagcat aaaccaattc agaatattgc cgtaaccctg
     3241 ctgggtatta tttatattgc tgtcccgctt agcctggtaa actttattgt aataaatggt
     3301 tcctccacaa gaatttccta cagcccaaac attttgctgg gattactttt tcttatttgg
     3361 agcaacgata ccggagccta cctgctgggc gtgagcatgg gaaaacacaa actatttcct
     3421 cgtatttcgc ctaaaaaaac ttgggagggt tttgtcggcg ggctgctcct caccatgatt
     3481 gtgtcgtggg tagatgcaat aattttccca gaggtagcct ttaaacattg gcttgtgatt
     3541 ggggtcatca cagctatagt tggtgctttt ggcgatttgg tggaatcaat gttcaagcga
     3601 agtgttggag tgaaggattc tggtaagttt cttccaagcc atggcggatt tctcgatagg
     3661 tttgatgcct ttctgctggt tattcctttt gtttacgcct atcttgaggt aatgatgtta
     3721 atttaattac gcttacccat gaagatacac cgggagggac tcgtttttct ggctgtaagc
     3781 cttattattt tggcggcgtt aaacgtagct gttggcctta ttttacctga agttacatac
     3841 tttttagctc ccctttcgtt actgatactg ctgtttctgg tcagattttt caggattccc
     3901 atgcgtggct tttcgcccaa caacgagtat atttactccc cagctgatgg aactgtagtg
     3961 gctattgagg aggttgaaga gaatgagtac cttaaaaccc gttgcattca ggtttcggta
     4021 ttcatgtcaa tttggaatgt tcacatcaac tggttcccca ccactggcac cattaaatac
     4081 tataagtacc atcccggcga ctacctggtg gcatggcatc caaaatcatc gtctcacaac
     4141 gaacgaacta ccgtagtagt tgaacgtagc gatggaacca aaattctcat caggcagatt
     4201 gcaggggcgt tagcacgtcg gattgtgtgc tacgcaaaac cgggccaatc aatagagcag
     4261 ggctcggagt tggggtttat caagtttggc tcaagggttg accttttcct acccttaaat
     4321 gcaaatgttg aggtgaagtt gaacgataga gttgtgggca agcaaacggt aattgcccga
     4381 ctttagcagg tttaatcggc atagctcgag aaaactatct cccttacctt tcccttgttg
     4441 ttaaagataa agttgaacgt tctaccatag tactcgttcg aaatcaccag ggtatcgctg
     4501 cttgactcat taatgttgct gattgcctta agcagctggt cgcgggtaag cccaaccgag
     4561 attccgttta ccagcttaat ttccggattt acaattttcc cggccatgag catttcccga
     4621 ttaaagtagg ttttgtaaac aaacagctca cttttcttgt agctaaactt gtaaatggtg
     4681 tctgttctgc taggctcatg aacattgcta cgagcaaatc gggttatttt tgttcgcgct
     4741 ggaagctggg tggtgaatgc ttgaatactt tcgtcgtggc caaatggtgc cttgaggaat
     4801 tcctgcttgc taatgccctg attggttgtg gttaccttgc tcacgctgtt acatgagttg
     4861 cctgcaacga ggagtaaaac tgtgtagcac gctattttta ctgcggtatt catggttgga
     4921 attttttgct caaacaaatc tacacttttt tgtgaaattt gtggcaaaat tgggtaatct
     4981 ataaaaagaa agtaaccact ccaacaaccg atacaattgc tcctgcaact tctctaaatg
     5041 ttattttctc tttaaggaga accgccgatg gtgcaataat aagcaccggg actatggaca
     5101 ttagggcaga ggctacaccg gtgctggtgt agcgaacagc tagcagcgag aaagatactc
     5161 ccagaaatgg cccaaagaac gatccaaccg ttatccgacg catagccccg gcgtttttta
     5221 cggctacaag cagattgcca gacttatgca tgaagagcag cactgcggca aagcctacta
     5281 ttccggtgat aacccttatt tgaaccgagg caactacgtc gtaacccgcc attccatact
     5341 tgctgagaac cagtccgcca gcctggccaa ctgcaccacc aaatgccagt aacagcccgg
     5401 ggattgaata cccgaacttg attttacgac cgataatctt actgccgttg ggggtctttt
     5461 cgcttctaag aactaccatt gcaatgccaa tgaaaatcag cagcatgccc agggcttgct
     5521 ttagggtgag cgtttcgccc attgatagcc agcctattcc cgcagcaatt ggcggtgaca
     5581 acgacatgat aagcatcgaa atacgagcac ctaccagcac gtatgactga aacaggaata
     5641 gatcacccat aacaaagccc accaagcccg aaagcgatag ccatagccac tgatgccctc
     5701 cggcatcagt aggcaatagt aaacctctgg caaataatga gtaaaggctt aaaaaaatga
     5761 aggccagaac cagcctgata aaatttaccg atagggagcc tactgttttg cttgccgatt
     5821 caaaggccaa cgaggttact gtccaaaaaa ctgcggttag caatgcggcc aactcgcccg
     5881 tgtgcgaact tatgaaatcc actttttttt aaatttgctt ccccgttgga gaacaatttc
     5941 cctgaatagc ttaatcggtc gagaaaattt atttcgtcat gctaaaaatc agcccaacaa
     6001 accaggttga cctgttggta tgctaaaatt tattcttata cttgcactat cctttccaga
     6061 agatacggtt agctgtaagc agcatttgta gaaaatttca tgcgatgaac aagcttgtta
     6121 agtacatcat tatcgttttt gtagttgcag cggtagcctt tcttctctgg tatttctcat
     6181 ctattgtagc ctacattctg atctcagcag tgctgagctt aatgggtaaa cccattgtag
     6241 atggggtttg taggctgaaa attaggaact ggcatccacc caaggcagta ggcgctacct
     6301 gcgcgcttat tgttatctgg gtgctatttg tggcattttt tagctttatg atacctctgg
     6361 tggtttcgca gtttaactcc cttagcggag ttgacgttgg cacactagtc aataggtttg
     6421 cccatccaat tgccggactg caggaattca tccaaaacta cctaccagct agcgccaagg
     6481 agttttccat tgaggagtac ctgacatcaa aaatcagcac cttttttact acatccatga
     6541 ttgccaacct gttcagctct acggcgaact tcgtcgttaa cctgggcatc gctctttttg
     6601 ccatatcatt cattaccttc ttcttcctaa aggacgatac ccttttttac aacggggtaa
     6661 tcctgctttt cccgtcaaga tttgaacagc agctgtcgca tgctttatca agcataaaca
     6721 ggctgctccg acgctacttt attgggctcc taatagagag caccctgatc atgctgcttg
     6781 ataccctctg gcttgtactc attggtcttc attttcaaac ggcaatagtg attgggctga
     6841 ttatcggcat tctaaacgtt attccttatg ttggccccct tatcggcatt ataattggaa
     6901 cgctaatagg gttggcttcc cagctgcagc tgatttcggc taccgaattg ctgcccctta
     6961 ctctttggat ggttggagcc atgcttgcca ccaagctaat cgacgatgcg gtttttcaac
     7021 ctgtgattta tgggaacagc gtcaaatctc atcccctcga aattttcctg gtaattctta
     7081 ttgcgggcag ccttgccggc gttctgggaa tgcttctggc aattcctgcc tacacggtac
     7141 ttagggtttt tgccaaggag ttttttaata acttcaggct ggttcaaaaa attacagaga
     7201 aaatttagcg tttactgatg taaaagtgta tttttactgc tataaaacaa ctatatgttt
     7261 gtaacttact agttgataaa cgcttcactc actatagatt tatcaggaaa atttaacaga
     7321 aaacctcatg ctcgtaaatt ctcatctaaa aaacggcttt ttgctgctgg caggtctttt
     7381 ggtatcgaat gcagcaattg cccaggagaa ccttgaagct gactccattt ttacctggaa
     7441 gctggttgag cttaccaacc ccagctcgca tagctgcatt caatttattc cccgagtcga
     7501 tattactccg gttagctggg acttcggcga tggaagtcca gcctcagccg acgttagccc
     7561 tgttcatact tacacctacg gcagctggag cgatagcgtt gaggttacgc ttacctatac
     7621 cgcaggcgga gccaatacgg ttaaaacccg tcagataccc ttaagcccgg cctactttca
     7681 ttgtattcat gactccaatc tgggacaagc ggcctccttt aagtatattt ttaggaatgc
     7741 atacatgatt gataacaacc caggtaacct tggaaatctt catttctcat ggaccattga
     7801 ttcagaaccc cttgccgaga atacctttag caatccggac ctggggcaat ggccaaatat
     7861 ttactacacc tttttaaacg gaggaaccca ccaggttaaa ctcgaagttt acaatactac
     7921 taatgctaca gaagttgccg gattcaccca taccctaact gtaactcccg acctttcggc
     7981 tggcgtagtt aagctggaaa atattcccaa cgtttttact cctaacggtg acggggtgaa
     8041 tgatacgttt acggttaata ctagcggaac gggctggttt gtgcttagaa tcctgtcccg
     8101 ttcgggtggg ctgctctata aaagtgagtc gagcacactt atatgggatg gccgtaacag
     8161 ccagggagaa gagttgccag agggtattta ctactatata attgaggata caaccggcca
     8221 atacgaaact gcaactgggt ttatctacat tttcaggggg aaaaagtaac atcctgtaaa
     8281 tttaacgtat ccatagctgt tgggctcatg cggatgttta tgaagcctcg taacacctca
     8341 ttagggatgc aggcagggtt gaacggcaga tttacctgta agcgaaaggt aattaacttg
     8401 ttaacttaat ttagaaaact atggctttac ttgaagaatt tgagaaacag gggaactggc
     8461 tattcaggta caggggcgtg ctaccactta taattttggc tgtaggggct ttcatctaca
     8521 tacgagctga acttaaccct agcccatcat catttatgga ggttaatagc tataagtcga
     8581 tttacgaaat atcctgcctg ctcgtcagcc tctttggact ggccataagg gtttacacaa
     8641 ttgggcatac tccaagcaaa acttcaggtc gaaacataaa ggaacaggta gccgaatcgc
     8701 tcaacactac tggcatatac tcagtcgtca gacacccgct ctacctcggg aattttttca
     8761 tgtggctagg tcctgccatg ctgactgcaa acttttggtt tatcgtttca ttctgcctgg
     8821 catactggat ttactacgaa cggattatgt ttgccgagga gcaatttctt aggcgaaaat
     8881 ttggcgaagc ctatacggag tgggctagta gagttcctgc ctttattccc tgttttaagc
     8941 attttaagaa gccgtgccat tcattctgca ctaggaaggt ggtggctaag gaaaaaaatg
     9001 ggttgctggc tgttttccta attttctgct cgtttaatgt agttggcgag cttgtgcata
     9061 agggtaagca ccacttcaac atgattttta ttgggatgtg catcttgtct atagtgcttt
     9121 acatagttgt taaaattttc aggaagaaga taaatcttgt aaagcagtag taaagacaat
     9181 ttgctaaagc tggttttccc tgcttacatt ccagttataa cttgtaaatc aacataatac
     9241 cgtcgcgtaa ggggagcagg acgttttcta cccccatatc attttgaacc atggtgttga
     9301 acgccctcag ggctttggtg tgctcgtcgt tttcactagc atcaaccact ttccctcccc
     9361 agagcacgtt atctgcaata attaaccctc cgctgttaac cttgggtaga acaagcttgt
     9421 agtactcggg gtactctctt ttatcgccgt caatgtaggc aaaatcgtag gtgtagtcaa
     9481 gggtagggat aatctggcag gcatcgccaa ggtgcagcct gattttgcta tcaaccccgg
     9541 ccatggaggt gtaggtttgg atggtctcct ccaactcgtc gtttacctca attgtatcaa
     9601 taactgtgtg gggtggagag cccagcgcca tgcaaattga ggtgtatccg gtaaaggttc
     9661 caacctcgag tatccggttg ggccgagata tgttgcaaag cagctgcaga aacttaccct
     9721 gaattggccc gctaatcatg cgagggttac aggtggaaag gtaggtgaac cgggtcagct
     9781 ccatcagggt atccgccatt ggcgaggtat ggcggctcag gtattcctcc atgtcgttat
     9841 caaccttagc catatctacc ggtatattaa cgttgaaagc ctgttttctg aaagaatttc
     9901 gttggcaacc tcgagtatgt cggacgatga aatgttttct atgcggttga aaacttctgt
     9961 caggtcttcg ggtttttggt aaaccataaa actttttgca gccgaaagca tgaggttttc
    10021 gttgctatcg gcagcaatgg cggtttgccc aataagctgt ctttttgcct taaaaagctg
    10081 catcaccccc attcgttgct ccctaattct ggcaagctcc ttaagaattt catggtgcga
    10141 tttttcgaca ttggccttat cggtgccaaa gtaaatggtg aacactccgg tgtcggtgta
    10201 tggtgtgtat gacgattcca cgttgtaggc aaaaccatgc ttctcgcgta gtgccaggtt
    10261 gagccttgag ttcatgccag gccctccaag gatgttattg agcaggtgca tggcaatgcg
    10321 ctttgggttt tgcaagtcgt aggcagttgt accaataatg caatgggtct gaaatgttga
    10381 ttttttaatg attttatctt ggggtctata gttgccgagc tctaaacggg gtttactcct
    10441 tttgttagca gaaatgccgt ttaggtactt gtctgccaac ctgctcacac gtgtaaaggg
    10501 aatatcgcct accgaacaga ataccatttg gtcggtgttg taacagcggc ttatgaacgc
    10561 tgcaataccc ttgcggttaa acctgcttac atggtttttg gtgcccagaa tattcctacc
    10621 aaaggggtgg ttagggtaga taagctcctc aaagtcatca aatattagct cgtaggggct
    10681 atccttatac gagtttatct catctattac cacctccttt tccttggcta tctccttttc
    10741 gggaaaggtg gcatgaaaag cgatgtcggt aatgagctcc atggccctgt tgaagtcctg
    10801 ctttagaaag ctggcgtgaa taaccgtttc ctccttggtg gtgtaagcgt taagttcacc
    10861 accaacgtcc tcaaggcggc tcatgatgtg aaaagccctt ctttttttgg ttcccttaaa
    10921 cagaacgtgc tcaataaaat gggcaatacc atgctcgtgg tccagctcat cgcgagttcc
    10981 tgcgtttatc agaatgcagc agtgggcaac aggtgatgaa acccgtttgt ggacaatttg
    11041 aataccgtta tgaagggttc tctgctcaaa ttccattata ccccggtaaa tttgaatgca
    11101 aacctactat aaataggcaa cttgtcaaag taaaagagct gccgctaaaa tgtggcatac
    11161 gaaaggtatc cgttacccaa gcccgtaatg taacggcttt ggttactgcc atcaaggtta
    11221 tatccttagc attcagctac atgttgggtt gaattgcatg gaagcaccta ttaccggtag
    11281 taaaattatg gaaccaatgt ttgcagatac aaaaatatcc tatatatttg caccgcaatt
    11341 gaacgttggt gccatagctc agttggtaga gcaaaggact gaaaatcctt gtgtccgtgg
    11401 ttcgattcca cgtggcacca cgtaaaacct ccggatatcc ggaggttttt tgatttaaag
    11461 gattttacaa actcttgttt accctgtttt tttacactgg aagttgccgg ggctaagcca
    11521 gtgttgttca tttatggatt atccatttac aggttttcgc tttaatctgt tatggataag
    11581 tataatttgt tgcctggaaa atgaaaatgc aggattggtt taaaagggtt tttatactcc
    11641 taaggcatag gtagaatctt atttaagtgt taaattccct gttgagatgc cttttcaggt
    11701 aggtggcggt gtagccgcat gccgactcaa taagctcctc gggggtacct gtgcaaacca
    11761 cctttccacc ttctgctcct ccctccgggc caaggtctat aatgtagtca gctaccttta
    11821 ccacatccat gttgtgctcg atgacaatta cagtgtttcc cctgtcaacc agcttgttca
    11881 gcacgtcgag taggatacgc acatcctcga agtgaaggcc ggtagtgggc tcatcgagta
    11941 tgtagagggt tttgccggta tcccgtttag ccagctcggt tgaaagcttt acacgctgag
    12001 cctcgccacc cgaaagggtt gttgacgatt gccccagcgt aatatatccc aacccaacct
    12061 cctgtagggt tttcagcttc tggtgaatct gggggatgct gccaaagaac tccaccgcct
    12121 ggttgatggt catatccagc acctcgctga tgttcttgcc cttatacttc acctcaaggg
    12181 tttcgcggtt gtaacgttgc ccatagcagt cggggcattt gatgtaaaca tcgggcagaa
    12241 agttcatctc aattacccta acgccagcac ctttgcacgt ttcacaacga cctcctttca
    12301 cgttaaacga aaaccggcct gccttaaatc cacgaatctt ggcatcgggt gtcagctcga
    12361 acagcttgcg gatgtcggtg aatactccgg tgtaggtagc cggatttgat cggggtgttc
    12421 tgcctatggg cgattggtca acctcaatta ccttgtcgat atgctcaatt ccatctatac
    12481 catcgaaatc gagtggttgc ttgtaggagt tgtaaaactg ttggctcagc gctggttgaa
    12541 gggtttcgtt aaccagggtt gattttccgc tgcccgaaac gcccgtaacg caaatcatgg
    12601 ttccaagcgg gaactccaca tcaatatttt taaggttgtt gcccctggca ccccttatgc
    12661 ggacgatttt cccatttccc ttacggcgtt ttgtagggac agcaatctcc ctttcgcctc
    12721 tgaggtactg ggcggtaagg gagttggttt tcattacctc tgcaggtgtt cctgttgcca
    12781 tcaccctgcc tccatgacga ccggctaatg ggccaatatc caccacgtaa tcggccgaga
    12841 ggattgtttc ctcatcatgt tctaccacta taaccgagtt tccggaatcg cgcagctgct
    12901 tcagggagtt aatcaacctt acgttatcgc gctggtgtag cccaatactt ggctcgtcga
    12961 gtatgtatag gacgtttacc agcttcgacc caatttgggt ggccaggcga atgcgttggc
    13021 tttcgccacc cgaaagggta accgttccac ggctcaacga caggtatcct aggccaacat
    13081 caagcaggaa gcctacccgt tcgcggattt ccttcagtat ttctgctgca atagctcgtt
    13141 gccggctgtt catcctgtcc tccaacccct gtagccactt cgagaggctt acgatgtcca
    13201 tggaggcaac atcggctatg cttttaccgt ctattttaaa ccaaagcgac tcgtttttaa
    13261 gtcgggagcc gcagcaatgc gggcaggtaa cctgacggat aaactggttc gcccacttgt
    13321 caccctttcc gtttagctca tcgtttgagc tgtggttctg cacaaagcta actacccctt
    13381 caaaacttag aaagtatgaa gaagtaagcc ctaaccccgt atgcttcagc ttgaattgct
    13441 cctcggaacc ataaaggatg acgttgaggg cttcctcggg gatggtagaa attggatcgg
    13501 aaattgaaaa cccatactta gaagcaattg cctctatttg ccagaatatc aacgagtttt
    13561 tatagggtcc aaggggaaga attccgcctt ttttaatgct tacagaaggg tcgggtatta
    13621 tgcgggatat atcggcttcg gaaatgtaac ccaggccgtt gcagtggggg caagcgcctt
    13681 tcggggagtt gaacgagaat gtgtatggtt cgggttcttc gtaggatatt ccggtagtag
    13741 ggcacatcag gttgcggctg aagtatcgcc acgaaccatc atcggcgttg agcaccatga
    13801 ttgtcccttt gcccatgttc atggctgtgg ccaccgattg agcaatacgc ttggagttgt
    13861 ccggttcaac caccatcttg tccaccacca gctctatgtg gtgggtttta tagcggtcga
    13921 gcttcatgtt tggccgcatc tcgataatct catcgtcaac ccttacctgc aggtagccct
    13981 tgtgccgaag ctgctcgaat agctccttgt agtggccttt tcgtcctcga atgagtggag
    14041 caagcaaaag ggttcggctg ccggtaaatt tttcaacaat taggctgata atttgctcgt
    14101 cggtgtagcg aaccatctcc tcgccggtat tgtaggagta ggcggttgaa gcgcgagcgt
    14161 agagcaaccg taggaagtca taaacctcgg tagtcgtgcc caccgtggaa cgtgggttgt
    14221 ggctggtggt tttttgctcg atggaaatta cggggctaag gccggaaatt ttatccacgt
    14281 cgggacgttc gagcgttccc agaaactgac gtgcataagc cgacatggtt tccatgtacc
    14341 ttcgttggcc ctcggcgtat atggtatcga aggccagcga tgactttccg ctaccgctca
    14401 aaccggtaat aaccgttagt tttccacgag gtatggcaac atcaatattt ttcaggttat
    14461 gcacccgggc gccctcaacc acaacttctt cttcttcacc cttcaaaaat tccatttcta
    14521 actccaaatt agtcccctat tgctcgtcct tcttccctgc tgcgtaagct tcaaccctga
    14581 aatttatatc cggaatttta attctgtagt gtttccctgc cttgttgttc aggtagcttt
    14641 cgcgtagcca tgggttgaaa agtttaatta ccttgtagtt ggtatcaaac cggcgggcga
    14701 aattagaaat gctggagatt gccgtatcaa cgctaacctc tctataccta aacggggggt
    14761 aaaggtcagc cgaatcaagg aaaaaaccat acttacgggg attttcaaaa attaccttga
    14821 tggccaaaat tctataaaca tagcgaccgg tttcctcacc cagcaccatg ttgtagtatg
    14881 aatcgccctg ctgtcggtca atttgtttat ccataccatt ttggccgtag ttgtaagagg
    14941 cagcagccat ggtccagctg ccgtattttc ggtagctttt gttcaaatac cggcaggcca
    15001 cctcggttga tttttcaacg tggtagcgct cgtcaacctc actgtttacc tcaagaccgt
    15061 aatctttgcc cgtgccctcg agaatttgcc aaattccggc agcctttgat ggggaaacaa
    15121 cctgcatgag accgctttcg gcaacggcaa ggtatttaaa atcatcgggg attccatttt
    15181 tttggagaat aggctcgata accgggaagt accggttggc tcttttgagc aacataacgg
    15241 tttgggagtg ccaaaaggta tttacctgca gctccctgtc caggctttcg cgcacatcga
    15301 agtaatcgag tggaacattt tcgccggcaa aggagaggtt ttgggggatt gggatgggaa
    15361 aaatgtagcc atcctgaagc accgtatcgg gcttttcgat gggcggttta ctgtgtgcag
    15421 atggtataaa ggcaatgaat acatagcagg tagctactgc taacagggct atagctgctc
    15481 ctaaaaattt tcgattttta aacattttct aaggagtgtt ttgggcatgc agctttatgc
    15541 aaccgggcgc aaaattacct tttttaaaca aattgttgac atctataaac catgtaacct
    15601 atgccggatc atactgcatt catggcaaaa ttccaatgca ttcggcagta caaatggtag
    15661 ttggttggga taaaaaatct ttgtagaggt aagcctaacg gctattgggc aatgctgctt
    15721 ttaagccctt cgctaaccca ttcgttaaac tttccggtgg agtcggaata gataagatag
    15781 gcctcaactc cggtgtggga gttgacaaaa ttccggctcc actccaaccc gtttaccatg
    15841 aaggctgttg ccagggcatc ggcccgagca gcagttggcg cgattactgt tgcgctcagc
    15901 aggttgctca tggccggaaa tcctgttttg gggtttattg tgtgggcgta ctttatgccg
    15961 tttacctcaa taaacttacg atagttgccc gatgttgaca taccccgtga acctgaaaag
    16021 tagaatttgg cctgaaggtt ttcaccgggt aaagcgtttt ctacaggctt gtcaatgcca
    16081 actatccagg gtgtgcctcg gctgctgatc cccatagcca taatttctcc acccacctct
    16141 accatgaagt tgctaatttc ttttgaactt aggaacctgg ctaccaggtc ggaagtgtag
    16201 ccctgggcca ccgcatttac atcaatctga atttttgggt cgtctttctg cagtcggttg
    16261 ccagccatgt tgattttacc cataccaatc agccgctgaa gtgcccttat ctgtgcgctg
    16321 tcaatgccgg catgttgttc cgggccaaag cccaatgccc taaccactgg gccaacagta
    16381 atatcgaagg ccccatcggt ttcgtggtaa atagaatcag agatagcaat tacctcggcc
    16441 agcagggcat caaccgtatc aatctcgttt ctgtttagac gggagattat ggagctgtcg
    16501 ttgtaaaccg acatggagcg gtcaaccgca tgaaaaatcg agtcaaaagc ttttgagaaa
    16561 tccctgcctg ccgaatcgga gtaaatgata ttgaaggttg ttccctgcgt aaaaccattt
    16621 accttggtgt aggttgctgt ttgccgtgcc tggcaggaaa ttatcagcag caacagcagc
    16681 aataactggc caaatcgctt taatgtggct atgtgaatat ggtgtgtagt catagtttgc
    16741 gtaagaagta tttaaattgc acaaacagca tgattaggta cgggataatg gctgataggc
    16801 catgctatta ggcttaaccc tttcttcaac caatcaatct aaattataaa atctagcaga
    16861 ggttaagacc tatagggttc aagcgcacgt ttgagtattg cctccacaac ttcatggttg
    16921 atgttctttc tttcacccag cttccatccc cgcgtgcgga acctctcaac aattggagca
    16981 acatcttcct gctttaatcc gtactcgcac aagcgggtct taatccccag cgaacggaaa
    17041 aaatcttcgg ttttgtttat tacgtttcgt gcggttaaat ctgctccgct cagcccccat
    17101 accctgctgc cgtactgtat aattttttca tgtttgggtt cctgcagctc ggtaagtagg
    17161 gccgggtaaa ctgcggcaag ggtggcacca tggtcaattc caaaaaatgc agtgagctca
    17221 tgcccaatca tgtgggttgc ccagtcagtt gttacgccag ttgatagcaa gccgtttagg
    17281 gccatggtgc agctccacat gaagttagcc cgaagggagt aatcggatgg atttttcagg
    17341 gtttgcgggc caacttctat gagggtttgc agaattgact ccgaaaagcg atcctgaata
    17401 ggcgagtcgg aggggaaagt catgtactgc tccataacgt ggataaaggc gtcggcaata
    17461 ccattagcta cctgatgggg agggagcgaa aaggtgaggg tagggtcgag aacggagaat
    17521 tttgggaaca gcaccgggct tccaaagctg tacttctcgc gggtttcaag acggcttatc
    17581 accgcacccg agttcatttc ggaacctgta gccggaagcg ttaaaacaac cccaaatggt
    17641 atggccttgg ttactactat attctttatt agcagatccc atggatcgga cccttcgtat
    17701 aggctggctg ctgcaataaa ttttgttccg tctattactg aacctccgcc aatcgccagt
    17761 atgaagtcaa cattattttt tttaaccacc tctaccgcct tcatcagggt ttcgtacttg
    17821 gggttggctt caattcctcc gaattcaaaa acgctgaagt tggaaagtgc cgccttaatc
    17881 ctttcgtagg taccgttttt cttaatgctg ccaccgccat aggtcataag aattctatcg
    17941 gttgggttaa gctgcattcc gattttttcg attttgtcgt ttccgaaaag aattctgacc
    18001 gggttaaaaa attcaaaatt attcatatcg tgttgttttt atggtattct gtcaaggggg
    18061 ccatgttgga aatctactag caatctaaat ggctatttga aaataacagc atggctgctt
    18121 gctacaaaat tagtaaatct ctgcggaaag gattcaagcc ggtatggcat aaatttttag
    18181 ggattatcct tgttggacaa taaagataat tccagctacg agcacgcccc ttccaaacct
    18241 tctcgctagt caatacttta aaagatttaa aaaagagtcc ggaatattct aagtataaat
    18301 ttagtagctt tatcaggcaa aaaataaggg ggtggctatg aataaactta ctttaggaaa
    18361 atatcacatc cttgtagtcg tggctgttct ggcagccacc gttgtgctcc tttcatgcag
    18421 gcagagcaag caggaaaatg taaaaagttc tgctgataag gccagagaaa acgtggtgag
    18481 ctggatggag tcaatgaaat tagagtatcc tggttaccag ccagccgaat ttggcgagct
    18541 aactcccagg tacaacaaaa gcaacattac tttccacctc tataacctca tagagcagga
    18601 aaggagcaag ccaagtccaa gcaaaagggc tatagacagc ctggagaacc ttttaaggga
    18661 taatcccggt gagctgcttg gctattcgat tacccataag tacaacgtaa aggatatctc
    18721 gggtgaaatc cggcaagttg aaagtttatt tcttctcgat acgttactcc gtgtaattac
    18781 ggtgcttgat gcggatgcct acgaccagat aatggatgag gagctatttt tcagacccga
    18841 aagtaaagac tcaactctat aagcatttag gctcttttta aaggcagcct ttaaaacctg
    18901 catattcacc tttacgatga acatgcaggt aattttgcag tgctgcttac cctgcaaaat
    18961 aatcatggtt gctttggact aaacttatag agttttgcat ggtaatgccc aaattcatat
    19021 ctcgataatc agaatttcca cttgggagca aaggcgtatg aaagcattaa ctcatgtgtt
    19081 ccggtgttga ttctggagag tttattaagc gagaaatcga aggcatagcc gaatgatagg
    19141 tattcgttaa ccttcatatc gaagaaaatt cccagcgcat cgccaatacg gtagtgggtg
    19201 ccaaagtaaa atttgctctg gtagagaaac tgggcggtca ggtcgcacga catgggtagt
    19261 ccaatcattg cacccattag cattgatggc atgaaaaccc agctggggct gatttccagg
    19321 ttgtagcccc ccatgaggta gagtggactt ttaagggttt tgtcgaactt tttattaaat
    19381 gaagtctgaa ggaaacgtgg tagggaaagg cctgcgtaga attcgccgga gtaaatgtaa
    19441 gcacctgcac ccaggttagg gtagaatcgg ctttcattct gggtaaactc cgggtcgttt
    19501 gggtcgttta tctgaaggtt ggtaagcgag gcatggtagt aggtgaaacc tcctttaatt
    19561 ccaagcgaaa gagttatttc gtcggttagt ctaaggcggt atgcatacga tgccgagatg
    19621 tgtgtattgc tgaccggcca tgtgtcatca ttcattatgt taaggcctat tcccatcctc
    19681 ttttcgggcc agggcatgtg ggccgaaagg ctgaaggttt tgggagctcc ttccaggcca
    19741 acccattgta acctggaaag gaggcttagg ttaaggtttc gtaccgtacc ggcaatggcg
    19801 gggttaatgg acaccggatt ctgaaagtag tgggtgaaaa ttggctcctg ctgtgccctc
    19861 agggtggttg ccgaaacggc aaccactcct gctgttagta aataggctat ttttctcatt
    19921 tttcaaaaaa atatcgactt taacttgatt atcgcttaat aaacacgctt cccttgtagg
    19981 ttttacctgt atctaaaatt tcgagcaggt agtagtaggt tccggcagga agtttagagc
    20041 ctagcgaaag ggatacgtta gctgttccgt cccagtcgtt tttataccta tcgcttttgt
    20101 aaaccaggtt accccaacgg ttgtaaacct tcagggtaat gtgttggaat tgccctatat
    20161 ttagaacttc gaaggtgtcg ttgaatccat caccgttggg tgagaaggcc tcgggtacca
    20221 caaccttccc gtcggggtcg ggtacaaccc gaacagttat gtatgccacg ctgctctcat
    20281 cgtccctatc ggttacccgg taggtaaatg tctcagtacc aacaaaaccg gtaagtgggg
    20341 agtagtaaac cttgaaatct gaggttatac ggcatatccc attggttggt gaagtttcaa
    20401 ctgccaacgc ttttactcca tcgtttaacc cgatatcgtt ggttaacacg tcaatagtgg
    20461 tttcggtatc catgaaaatt tcaacatcat catcattggc ctgaggtgta aagttctcac
    20521 ggttgagaac atctataact accttagcca ggctgtagtc gccatccaca tcagcaacat
    20581 agtaccaaag cgagtcaaca ccaaagaagt tgttcgcggg ggtgtagagt atggagttgt
    20641 cggggttaac agataccgct ccgttagtag ggttgctgaa cacaaccgtt ctaattcccc
    20701 catcgttcag gttcctgtcg ttaaacagta cattaatgga gatggccttg ttcttaaaag
    20761 tagtaacggt atcgttgtag gcaataggta caaggttggc gtttttaacg ttgatggtaa
    20821 ccgcagcgct tgagcagtcg ctgtcgaagt cgcaaacctg gtagtagaat acgtcctctc
    20881 ccaaaaagcc agtgttgggt gtgtaggtta ccgtgttatc tgcgttgagc agcacatttc
    20941 cgttaatggg gaatatgttg agcagcacct ttataccacc gtcctccagg ccggtatcgt
    21001 tgaaaagaac atctacggta acgggggtgt tggtagttgt accccgccca tcagggttgg
    21061 ctaagggttt gtggtcgggc ctttcgttta cggtgatgta tagctttcct atgtcgtaat
    21121 cgccatcctc atcagctacc aggtaggtta acgagtcggt tccaacaaac cagctggatg
    21181 ggaaatactt gatgttatgg ttgtcgaagg cgtaggcaaa gccatgaagc ggtttaccca
    21241 ttaccttgat gtagtcgagc ccatcgtcga gtccggtgtc gttgttcacc acgtcaacaa
    21301 gcaggggaac gttcatgttg gttgaggcgg tgtcggcatt ggccacaggg ataaaattaa
    21361 cctccttgac gttaaccgtt acggtagcca cattgctggt gtcgttgttc ctatcaacta
    21421 taaagtactt aagtgttgac agacccaggt aatcggtagc cggtataaat gataccatgt
    21481 tatcgggttt aaccgcagcg gttcccgtag ccgcgtcggg aaactcatca atgaataccc
    21541 ttatccaacc gtcatcaagc ccatcatcgt tgatgagcac gttgatatcg agtggggtgt
    21601 ttttcgatgt agcccgatga tcatcattgg caaccggaat ggcgttttgc gtggcaagaa
    21661 ccaccacgta aaccgtagca atatcgtaat cgccatctac atcctcaaca aggtacttaa
    21721 actcatcatt tccaataaag aagtatgagg gtgtgtaggt aatcgtatgg ttgtcgttaa
    21781 cctgaacagt tccccatttt ggggcagaga acacctggag gctctttatc ccctcgtaga
    21841 gcagggagtc attctcaaga acatggatat caaccggttg gttaacgtag gtttgcacgt
    21901 aatcgtcaac agcaacaggg atcatgttta caggtttaac gtcgataaat accgtagctt
    21961 catcccagtc gccatccctg tcggttaccc tgtagctgaa atggtcggca cccaggtagc
    22021 tgttttgtgg tgtataggta accgtattat ctgcttcaac aacagtagtt ccgtgagagg
    22081 cttggttggt gattgaaacc tctacaggag tatcgtccag gccggtatcg tttaccaaaa
    22141 catctactat tctaggcgtg ttaagggatg tggctctgta gtcatcgttg gccacgggta
    22201 agtagttggg gcgttcggtt acctcaacgg taactgttgc gatggagtag tcgccatcca
    22261 catcctctac ccagtacctg aatgtgtcgg ttcccaagaa catgtatgag ggagtgtagg
    22321 ttactgttcg gttttcgttc acagtaacag tgccaaaaga gggcatttca aagatgatga
    22381 gtctttcgaa gccatcgtct agtcctgtgt cgttagccag aacgtttatg tcaacatttg
    22441 tattgataat ggttgaagcc gcatcgtcgt tggaatgggg tacataattc tcaccttcct
    22501 tgacccgtat ggtaaccagc gcatcgtcgc tatcgccatc ggaatcggta accgtgtagc
    22561 ggaaggttgc aataccggtg aaagcagctg gaggggtaaa ggttatggtg ttgttaggat
    22621 taacaaccgt gctgccgatt gtgggcagct cagaaatagt aagcgttagg ggaacatctt
    22681 ccaaccctgt atcgttgact agaacatcaa caaccacagg agtttcaaag ctgcacccgc
    22741 ggcggtcgtc gttggctacc ggtatggagt tgggtttttc aatgaccgta atggttacgg
    22801 tgccaatagc gtaatcgcca tccacatcgg ttaccatgta gcggaaggtt tcaactccaa
    22861 taaacatata gctgggggta taggttaccg tgcggttaga gtttacaacc acacttccga
    22921 acttaggctg ctcgaaaact gtcagcgagc caaacccgtc gtctaaacct gtatcgttgg
    22981 ccagaacatt cacaacagca ggtttattca cgagggttgt tgcattgtcg ggaacagcat
    23041 acggcacaat attctgacca gccttcacat ggatggtaac ctgtgcatcg tcgaagtctc
    23101 catcaacatc agtaacagta tagcggaagg ttatagttcc ggtgaagtta gcctcggggg
    23161 taaaagttat ggtgttgtcg gggtttggaa cggcgctgcc gctttggggt gcttctgtaa
    23221 cggaaacggc aagcggcaca tcatccagtc cactatcgtt aattagcaca tcaacggtaa
    23281 cggccgagtt gaagctgcag ccccgcgagt cgtcgttggc aacgggtttg ctgtcgggct
    23341 tttcatccac aacaacggta acaatggcca cagagtagtc gccatccaca tcctcaacca
    23401 tgtagctgaa gctatcggag cctacaaaca tgtatcccgg gaagtaggtt agggttcggt
    23461 tggcattcac cacaacagtt ccaaaggcag gttgttgcct gatggttagt gcgccaaaac
    23521 cgtcgtcaag cccctcatcg ttagccagca cattgatttc tacggatttg ttgataaaag
    23581 tatgggcatt atcatcgcgt gccaggggta cagcatttat acctgccttc acattgatga
    23641 ataccatggc ttcatcgcta tcgccattgg cgtcggtaac cctataccca aaggtggtca
    23701 atccgataaa cccatcgaat ggagtaaatg taatggtgtt atcggggtta acctgtgcat
    23761 tgccatcagt tgggtttatg ttgatagata gggtcaaagg cacatcgtca attccggtat
    23821 cgtttgaaag cacatcaacg gtaacgctgg agttgaagct gcatcccctg taatcgtcat
    23881 tggctatcgg aacgtagttt ggcttttcga tgacggtaac ggtaacggta gccagcgagt
    23941 aatcaccatc agcatcctca acatagtagc ggaatgtttc aacgccaaca aacatgtggg
    24001 caggagtata ggtaacggat ctattggcgt tcaacacaac agtaccaaac tccggttgct
    24061 ggtaaatgta aagcccacca aacccatcat ctaatcctgt atcgttcgag agaacattta
    24121 tggtaacagg attattcata atagttgtgg cattgtcggc gttggcaatt ggaaccatat
    24181 ttacacccga tttaaccttg atggtaacca gcgcatcgtc gctatcgcca tcggcatcgg
    24241 caaccgtgta gcggaaagtc atttcaccta tgaatcctgt agcaggagta taagttaccg
    24301 tgttatcagc aggattcact atggcatttc ccgatgtggg ttgctgcgaa atggaaaccg
    24361 tgatgggtgt atcttccaat cctgtatcgt tgaatagcac atccacaacc actggtgttt
    24421 caaagctgca acccctacgg tcgtcattag caacgggaat atagttcggc ctttccttca
    24481 ccttgatggt taccgttgca atatcgtaat caccatccac atcggttacc atgtagcgga
    24541 aggtttcggt gccaacaaac atgtaggtag gcgtatatgt tatcgttcgg ttggcattca
    24601 ccacaacagc tccaaattcg ggttgctgga agatggttat tgagccaagc ccatcgtcca
    24661 aaccggtgtc gttggccagc acgttaatgc tctttggagt atttacaatg gttgaatcgg
    24721 tatcatcgtt ggctacaggc acaatgttta tgccctcctt aacgtttatg tggacatttg
    24781 cagtggagca atcaccatcc acatcgcaaa cctggtatcc aaatatttct accccaacaa
    24841 aatcggtatt tggagtatag gttatggtat gatcgggatt taccagagcg ttgccattta
    24901 caggttgggt tgtaaccgtc aagctttgga gcccgtcctc aagccctgta tcgttgatga
    24961 gcacatcaac tgttacagga gtattccttg aagccccccg gtaatcgtcg tatgcggttg
    25021 gaaggtggtt gggcacactg gttacggtga tggttacaac ggcaatatcg tagtcgccat
    25081 ctacatcctc gatgaggtat gagaagttat cggtacccga gaagccggca tcaggggtat
    25141 aggtaatcgt gcggtcaggg ttaaccacaa caatcccatg ggcagggttt gcaaatatga
    25201 ttaaacttcc aaaaccatca tccaggcctg catcgtttgc cagcacgttg attttttgag
    25261 ccgtatttat aaatgttgta gcattatcat caacggcatc gggtatgtgg ttaacaggac
    25321 gaacattgat ggtaacagtg gcctcgtcgg agtcgccatt tacgtcctca actttgtact
    25381 tgaaggttgc aactcccaaa tagcccgaag caggagtaaa ggttacggta ttgtcagcgt
    25441 tggccgttgc attcccctgt gttgggttgg gagcttcggt tattgtaacg gaaattccac
    25501 catcctcaag tccatggtca ttgaagagaa catccacaac acgtggagtg ttgatgcttg
    25561 ttcccctggc atcgtcaacg gcaacaggaa tgtcgtttac aaaggttacc gtaatggtaa
    25621 catttgcctc gtcgtaacct ccgtctctat ctgttacccg gtaggtaaag gtatcatcac
    25681 cattgaacag cccattggga gtgtaggtaa tggtattgtc gggttgaacg gctacgctac
    25741 catgggttgg atttgcagca attgtaacca ctattccatt cttatcgaga cctttatcat
    25801 tggccagaac atcaacctgc accgggtctt tttcaggcgt agttgctgca tcatcagctg
    25861 cacggggctt ggtgtcgtca agattggtaa ccgacaccga gggtatggac aagttgttgt
    25921 agttaggatc gctgcttgcc gatgaaatgg tcactgtata aggctgatcg ccatcttcag
    25981 ctaggtcatc tactcctgtt atggttattg ttgccgggct attccagctt gtattgttga
    26041 aaacttgctg ggttggagat actgttcctt cggtttcatc ggacgacgca aagcttattg
    26101 ttacattgct gaccggtgcg gtgttcaagc ttatctggaa ggtagcagtt gaaccatcct
    26161 cctttgtagt tagcccgctg acagggaaaa cattcacccc ggcctgatcg tcatcaatgt
    26221 tgattacagc aatatcatcg ggattaagcc cgttgtagtt cggatcggcg ctggttgctg
    26281 caccaatgat aaccgtgtag gttatatttc catcggcaac aacatcgtta acgccggtaa
    26341 cggtaaccgt ttgggcaacg ttccatgttg aggaagtgaa agtaactgag gctggactaa
    26401 cagttccctc ggccgtattg ctcgatgaca atccaatggt tacatctgca gctggctggc
    26461 tgttgagaac aatggtgaaa gtggccgtgg ttccaacctc acgagtgttc ccgcttattg
    26521 ccgaaacgtt aatggcggca acatcatcat cggtacaggt aacgttaaca tcgggtgggt
    26581 ttaatccgtt atacttacta tcaccactgg tggcagaata agtggcaatt gcaaaggtag
    26641 catcgccatc caccacatag ttattaaccg gagtgatggt tacaacttgc tcaacattcc
    26701 agttaccact ggtaaaggtt aggctggcag gagaagctgt tcccttactg gtgttctgcg
    26761 attccaggtt aaacgagacg tttgcggttg gtttagagga caaccggtag gttacgctaa
    26821 ccggggaatc agactctgaa taggtgatgt tttgctgtga gagtatatag cctgcattat
    26881 cgttgtcaat attggtaagc gaaacatcaa ccgggttaag cccgtcgtac ttcggatcgg
    26941 agctgatgca gggctgtgtt tgtatggtgt aggccacgtt gtcatcgtcc acgtcatcgt
    27001 tcataccggt taccgtaatg gtttggttgg tattccaatt gtcaggggta aaaggcaccg
    27061 tgctgggcga taccactccc tcgttggtgt tgagcgatac aagtgtaatg gagacgttgg
    27121 ccgtaggctt gctggtaagc ttcattgtaa aggtagctgt tcctccagcc tcggtggtgt
    27181 aaagaccttc gtatggagaa acagcaacac cagcatcatc gttatcaatg ttggtagctg
    27241 aaacatcggg tgggttgagt ccgttgtact tggcatcagt gcttgtagca gtgccagtgg
    27301 tgatggtata ggtcacgttg tcgtcgtcca catcatcatc cgtaccggtg atggtagctg
    27361 tttgaagcga attccaatta ccggaggtga aggttaaact tgtgggattt actgttccct
    27421 cggcggggtt gctcgaggcg agggtgatgg taacgttggc tgttggtttg gagttcagct
    27481 tcactgtgaa tgtggccgtt ccgcccgctt cggtggtttg cagccctgaa gttgggttaa
    27541 ctgtaaatcc ggccgtatcg ttgtcaatgt tggttactga aacatcggct gggttgagcc
    27601 cgctatactt ggtgtcggag ctggttgcag caccggtgat aatagtgtag gccacgttat
    27661 cgtcgtcaat atcgtcatcc acaccagtga tggttattgt ttgcgggttg ctccatgttg
    27721 ctggggtgaa tgtaacgctg gttggtatta cagttccctc gttggtattg cttgaagtga
    27781 ttgaaatggt aacattggcc gttggcctag tgttgagccg gatggtgaag gtggttattc
    27841 cacccgtctc ggtggtttgt agccctgaag tggggttaac tgtatatccg gccgtgtcgt
    27901 tgtcaatgtt ggttactgaa acatcggttg ggttgacccc gctgtacttt gtatcggtgc
    27961 tggttgcagg gttggtaata attgtatagg ccacgttgtc gtcatcaacg tcatcattca
    28021 caccggtaat ggtgattgtt tgaggggtgt tccagccagt aggagtaaaa gttaaacttg
    28081 ttggattaat agttccctcg gctgggttgc ttgaggcgat tgaaatggtt acatcggcgg
    28141 ttggcttgga attcagcttt accgtaaagg tggccgtacc gcccgcctcg gtggtttgca
    28201 accctgttgt tgggctaacg gtaaatccgg ccgtgtcgtt gtcgatgttg gttacggaaa
    28261 catcggtcgg gttaaggccg ctgtacttag cgtcggtgct ggttgcaaca ctagtgataa
    28321 tggtgtaggc cacgttgtca tcatcaacgt catcattcac accggtaatg gtgattgttt
    28381 gagaggtgtt ccatcctgta ggagtaaagg ttaagcttgt gggatttacg gtaccctcgg
    28441 cggggttgct cgaggagagg ttaatggtaa cgttggctgt tggcttggaa ttcagcttta
    28501 ccgtaaaggt ggccgtaccg cccgcctcgg tggtttgaag tcctgctgtt gggctaacgg
    28561 taaacccggc cacgtcatcg tttacgttgg ttacggtaac atcggctggg ttcatgttgt
    28621 tatagccggg atcggagctg gttgcattag aagttactat ggtgtaggca acatcgccat
    28681 cgtcctcgtc atcgtttacg ggtgttacgg tagcaacttt tggggtattc cacgttgtag
    28741 tagtaaaggt tacgctaacc ggagaaacgg taccctcggt caaatcgttg gacgagaggt
    28801 taatggtaac atcaccggat ggcttggtgt ttaggacgat ggtgaaggtt actgatgatc
    28861 cggcctcact ggtggtgaga accgttggag taacggtgat tcctgccaca tcgttatcat
    28921 tgtttataac cgaaacgtcg gcagggtcta gcccactata ctttgagtcg gagctggaag
    28981 cagtggccgt tacaattgtg tagctaacag ggccatcagc aaccatgtcg tcaactccgg
    29041 taattgaaat tgtttgagct atgctccacg ttgcagaagt gaaagtaatg gaagtcgggc
    29101 ttaccgttcc ctcagcagta ttgctggacg ataagccaat ggttacatcg gcggttggct
    29161 ggctgttgag aactatggtg aaggtggcct gcccgcccga ctcagatgtg gttagccctg
    29221 aggttggggc aaccgttata ccagcctcat cgttgtcagt attggtggcc gaaacagtaa
    29281 aacttcttcc agtgtagttc ccatcggagc tggaggccaa accgtttatg gtgtatgatt
    29341 ggttgccatc gtcaacaaaa tcgtcaactc cggtaattgt tactgtttgg tttgtgctcc
    29401 agttcgatgg tgtaaaggtt agggttgaag gcgagaccgc tccctcagct ggcttgcttg
    29461 aggtaagcgc aatggttaca ttagcagtag gctttgagtt cagcttaacc gtgaatgttg
    29521 cagaaccacc tgcctcagtc gttattaagc cgctgatagg tgaaactata atgccagcat
    29581 catcatcgtc aatatttaca actgacacat ctgaagcgtt cgtcccacca tacctggtat
    29641 cggtgctggt tgcaggtgct gtaactatgg tgtagctctg attgccgtca tccacatcgt
    29701 catttacccc ggtaactatg atggtttggc tcaaattcca gttacctgca ttaaaggtaa
    29761 cgcttaatgg tgaaacagct ccctcgcctg tattgcttga gcttaaacca actgtaacgc
    29821 ttgaaactgg ttggctgttg agccgaatgg taaagttggc ctgtccgcca ccctcggtgg
    29881 tggtgagccc ggatgttggt tgaacaacta tacctgccac atcattattt gtgttgatta
    29941 ccgtcacatc agccacgttg aggttgttga ataatggatc ggtgctggta gtgccggagg
    30001 tttgaatgaa gtacgttaca tcgccatcat ccacataatc atccacacca gtaacagtta
    30061 cagttttagg cgttgcccag tcggcagaag tgaaggtaag acttgcaggc gaaacagttc
    30121 cctcggcagt attgcttgag ttcaaggtga gcgaaacgtt ggctccgggt tgtgctgcca
    30181 gcctaacggt gaaggttgct gttccacctg cttcggtggt tgtgaggccc gaagtagggt
    30241 taaccaccac agcggctaca tcgttgtcgg tattcaccat gttgatatcc gggggatcaa
    30301 taccgttgta gtagctgtcg ctacttgacg aaggtccaac ctgaatggtg tagctgatgt
    30361 ttccgtccac cacgtaatcg tcaacaccgg taacggtaac cgtttgaggc gtgttccagt
    30421 ttgctgttgt aaaggtgatg gatgagacta ctgttccctc ggtcaaatcg gtactggtaa
    30481 actgaaggat cacgttggct aaaggttggg ttttgcatac cacggtaaag gaagcggtgc
    30541 ctccggcctc ggttgtattg gccgatatta cggtaatggt aaaaccgggt tcccttacgg
    30601 taatggttgc ggtgctggag agcggtacct cgcagttgcc gttatcagcc ttcaccacaa
    30661 aaaggtttga accaacagag aggttggcag ccgggactgt tatggtaagg tttgctcctg
    30721 ctcctactcc cgatgtgatt aaggtttcat ttttgtatat gttgtaggtt actcccgatt
    30781 gagcagaacc aatggttatt gttccattac gcccagggtc aaccgtgctg ccagtaaccg
    30841 tcaggccaac gttgggtgcg tttattaccg ttatagcaaa cgggttggag gttgctgagc
    30901 agcccgattt ggtaacctta agggtatagt tggtacctgc gtcactaagc gttgcgctgg
    30961 gtttgacgaa ctgttttgtg ctagaaacaa tggttgtccc ctttttccat tcgtaggagg
    31021 cgttgttctc ggtggcgctg aggttgattt cctcaccaac gcatacattt tgatcgccag
    31081 tgaaagttgt taaggggaat atggttacct ggaagttccg ggttgaaacg caactaccct
    31141 tactaaccgt tagcgaccag ctaccggcgc taaaactacc ccatgccttt gatagggttt
    31201 gggtgctagc caccacagtt ccgttgtact tccatgtata ggatgaggca cctacctctt
    31261 gagccaccag gtacaaatcc tgaccaacac aaatggtggt gtccccgcta aattccaaat
    31321 cggggccaac atatacagca aattcctgaa tggtttgcga tagcccgtca tcaaccataa
    31381 cgtaaataat ggcatggccc gatttgttgg ctacaggggt aacactaatg gtacggttgg
    31441 ttccgctacc gccaatggta atggagctgt ttggaacaaa ggttgggtcg tgagagtatg
    31501 ccgtaacggt aaggttggct gcgggagttt cgctatcgtt taccgtaaaa ggtatggccg
    31561 gggaggggtt gtttgtacac aggttttggt cggcaatgga tgatatggta ggaggtgtgt
    31621 ttacagtgat gtaaacctta tcggcgttca ttgaccagaa ctggctattc tttgcctccc
    31681 agtcgaactg ggtaatgcca acccatccgg tatttggggt aaaggttagg ttggccaggt
    31741 tggccagagt gatttcctgg ttcacagtaa ctgctgttcc tgacagctta agcgagccat
    31801 ttgccggtag cgattttata cggattcggg taaggggatc ctcaatagga tcaaaaaatt
    31861 tagttgtaaa atcagcttgt gtaaaggtta tagtattgtt gggtagtccc tgcttataaa
    31921 tatctccaac ccatggtgct aacccgttga tgcactgggt agtgctctgg ttcacgtgaa
    31981 tggtgatatc ggaatatccc aggtctatag cgttgaacca tgtattcatg gtatttaagt
    32041 tgccgttgaa gtttgtattt tcaaggttcg cattccacga ccatatacgg ggtatgatgg
    32101 tgctaacctt aagctgctta cgctgagttt cgtccataag cccatgtcct gtttcgtcgc
    32161 cccatcggga gatataggta tcgtcccagt atatggtagc gttaagcttc ctgaagggtc
    32221 ggatggaaga ggtgattacc ccatcgcact gctccacatc gtaaacagga aaatttgttg
    32281 ggcctctacc cataaaggtg gccgaagcat ggtaaacacc tgttgccacc tcggcgccgg
    32341 catcattctt gaagttccag aggatatagt tattccctct tcgcatgtta cggtatataa
    32401 cccgatcgtt acctgcagtg gagtagcctc catcgccgtc caggtctatc aaaatagcag
    32461 taaatgcatc aatgttggag tttatcttga aaatagcaat ggagctgtac tcacaaccca
    32521 ccttattctt ggtgaggtaa atgtcgtgat cagcaaaagc cagctgttgg ggagtgcgat
    32581 cgtactggta gtcgaagaag agggtatcgt tggcccaaac ctgagtttta ggagcggcca
    32641 accgtgtggt aggccaaatg ttcctgtcgg gatcgttgag gtagattcta tactccgatg
    32701 cagctgatgt ttgatcgtta tccttgcttt gtgccctttt gatgtagttt ggctcgttag
    32761 tgggggttat taaaccgtag gagttagact gaaacgtaaa cgaaaagggg tagtactgga
    32821 agttgatctt gttcacgaag tcatcggagg tgaaaacgta gaagtagtta ttcaccgggt
    32881 actgggtgaa gctggtggtg gttagctgcc atgtttttga ccatatccta cctgccggct
    32941 tgtttggctc gcctgggttg gtgataatgt tgttgttgga atcggttacc gtgatgtcga
    33001 agaactccag cgcaaaacga tagttattgt aatatctgaa ctcgatgtag tagtcgccgg
    33061 tttggtcggc agtgaaggtt tttgggctgt agccaccggt tatggtaaca ccgtttagct
    33121 tggcaccgtt tgggccggca atggcctggg aataatttgc aatgtatcct gctcctgatg
    33181 ttggaatatt cgattcggcc acagctatgc tgccatcggg cttccttatt ctgtagtagg
    33241 cgttattggg atttgtatat acatttcccc catagttaat agtgttcatc ttaaagccaa
    33301 agaaaacctt ctcgccggca acaagcctga tgttcagcct tttttcggca gaacagttgt
    33361 acccggcaaa gctgctgctt cctgtgccga acacgttaaa ctccagccat agcctgtcgg
    33421 aggagttggg catgagctgc ttggttccct cggcgtgtga aattccacta aacaactgca
    33481 gaataaaagc aatggttaat ccggtaaata gctttctcat atcgtgtacc ttaaattagt
    33541 tttaattgta tggcttgcca ttggcggttt gtcataattg ttaacttttt accaaactca
    33601 aaccacttcc acggtctggg ttaaattttt gttaagttta aatgttaggg gataaggttt
    33661 cccaaattac ccggtaacca ccaacgccct cccagtagta gtagaggtga atagtgctgt
    33721 tagtgggatc gtagtatgaa tggtaagcgg ggttgtaggg gtcgccctca ttaactatgt
    33781 agtcaaaacc aacaatattg aataccacag agttgtcggg gttaatggtg atatccattg
    33841 actctccgta gctgccccag agggcaaagt ttgtggtgca gctggtgacg gaaagggtga
    33901 ttagctgctt ttgggtgtag gtattaatgt acggtttgtc tgggtaagca ccgccaagtg
    33961 ttgggtgaaa gtaagtgatg tgcgaggtgt agaaaccggc atacgagttc gcaactagaa
    34021 tttttgtggt ggaacggtta agggattcga atccattgtc catcaccgat acgtacttta
    34081 aagcaaagta atcgcccaca tcggcgctgg cagcatcggg gggaaaatca tctatgccgt
    34141 ttcctagctt ggagtagccc tgcttcagct catcccatga gtagattttg gtaacataaa
    34201 aggtgtcggt gccatttgct ccgtttaaaa caatattatc aagcagcatt tctggagtgg
    34261 ctgaattatc ggccatgtga acatatctct taaatacctt cacagcaaca accggtggac
    34321 cctggtaaac cgaaaggtaa attgagtcgt cctggttaac ggcactgggc ttaaactgca
    34381 ctagctctga cgcatcaagg atacctcctg tacgcacgtt gctggtcatg aggtcctggt
    34441 tttcaataca ggaacctaac gacaaggtag taagggcaat gggcaaaatc agcttcacga
    34501 aatacatttt gaacttttcc ataagtttta gatattgtta tcttctaaaa tcagcttaat
    34561 tgcaatccca ccaaaccggg gtatttaggt tgtcgtctcc ctgcagtgca actgcttttg
    34621 ccacgttttc accatttcgc gagtactcgt cggatgggta gcgaagcctt acgggtattt
    34681 taccaccatt cagtccatcc tctgctggcg ttaaaaccgg atacccagtt cttcgccatt
    34741 caaaccaagc ttgaagtccc tggtcgaaca tggccaccca tttttgggta gcgatttttt
    34801 gtagtgggtt agcggcatcc catgcaccgt tatggttcag gaaatcggta ataaaatcgt
    34861 ctatgtttat gctaacccct tcaaacgatg ggtagtaaac gggtatattt tcaactattt
    34921 ccctggcata ttgcctgtat gaagcatcaa ttcccatttc gtagtaatcc tttggggtaa
    34981 gtgggctgat gtcaatcagg ttgcgttgtg cagcttcggc aagtatgaag tttacttcgg
    35041 cgaagctcat gaacatggca ggtgcgtcgg gtcgaataaa atactcccca atttttgaag
    35101 tacgcctgat tccattgcct aagtatgctg cagctttagc agatgtaaga ccgttgggaa
    35161 ggccaatcca gaagcctccg gcctgtggcg tttgggcgta aaccacaatt ctccagtcgg
    35221 ggttatcatg gttacctggg ttatacatga catcaacaag ggttctgctc accctgtggt
    35281 catcgcgggt tttgtaggtt tcgttcaagg ggtggttgtt ggggtaagag ccaaggtagt
    35341 gaagcgctgc attgtcagaa ttagaggtaa atattggatt gtcggtagaa tttcccaaaa
    35401 ttgaggacat ttctgacttg acgaactgct cctgttcggc atgacccgac atccggagta
    35461 aaatccttaa acgcaatgag ttggcaaatt tcttccattt aaggatatcg ccatgaaaaa
    35521 ggatatcgcc ctcaatacca tcgccataag ggttaagaag ctcgttggca tatttcaggt
    35581 tctgaatgag gttcaggtag atgctttcct gtgtatcgta ctgtggcgta agggtgctat
    35641 ctgggttgaa tgcttgggta tagggtatat cgccatacaa atccgtgagt acagaaaaaa
    35701 ggtatgattt cagcaccaga gcaacgccga tgtagttggc atggcctttt ctctggccta
    35761 tctcgtaaat tctttgaatt tcaattcccg gcccggcgta aaggttgttc caaacggaat
    35821 tgataacatc attgcgggga acatacctgt cctcatcggt gtactgaacc tttgccatgt
    35881 gctgaaccca gcatgagcca atttcgtgac ccagccacac atcgaaaagc tgatcgccca
    35941 gcgattcgac agcatgggtc agcagcatgt cggggtggtc agcatttgca ccaatcgcat
    36001 ttggattcct gtttagctcc tcaaaatcac cggtgcatga taccatgcct acaagtatgc
    36061 ccgttatggc aatccatcta tatatcttaa accatctcca cataggggta tccattattt
    36121 gtcagcatta aaactttaaa tttaaactta acccatagct acggggcgag ggcagctggc
    36181 caaattcaag gccttggaca ttgctgttgc tgaaagcggt ttccgggtca atatggggca
    36241 ctcgggcgta gagcagtgca aggtttcgac caacaacggc tatagaaatc tccttaataa
    36301 ggttattggc aatgtggttg aaggtgtagc caagcgatat ctcgcggagc ttcacaaagc
    36361 tggcgtcaaa aacgctggag taggcgatgt tgttgttgta ggcagccttg ttgaactctt
    36421 cggccgtaac tactacattg ttgggtaccc attcacccgc ttcgttctgc attacgccaa
    36481 cccctataat tccaccttca cgccctttta atgtttcctt taaaacacca gcgtatctac
    36541 cccaggttga agtcatggaa taaatctcgc caccaaatcg ggcatcaatg agaaagttga
    36601 gtgataaatt tttgtatcgt agcgagttag caatgctacc tgtccagtcg ggggtgtagt
    36661 tacccaaagc atgtatttcg ccctgtgcag gtaacccatc gtagaatatt atttttccgt
    36721 tttcattacg tttaaagtcg tacccataga gtgtgccaac tggttgacct gggattgcaa
    36781 gtacgtcgag gctccagtat ccacccaaaa cgtactgctt gagtccctcg gctaactcct
    36841 tcacctcgtt tttgttccat gcgaagttca ggtttatgct ccattcgaat ccggaacgtg
    36901 ttctcaccgg cacgccatcg agcgtaagct caataccgct gttgtctatt tcacccgcgt
    36961 taacaatttt ggatgtgtag ccggtggtgg cgctaacggg taggcggatt atttggtcga
    37021 atgtcttttg gttgtagtag gtaaggtcta taccaagcct gctcatgaac aatttcagtg
    37081 tagcacctac ctcaagagag ttagtacgct ggggcttaag gttggggttt ggtagatcgt
    37141 tgggaatgta aatagcaggc agcttggttg actcgtgcca tccctggtcg aagcggtaaa
    37201 ccggttgtag ctggtaggga tctgtatcag tgcctacctg tgcccaccca gctctgacct
    37261 gaaccatatt caaagtaggt gattgaaaat cgaaaagatc ggataggaca gctgaaaggt
    37321 tgaacgatgg gtagaaatag gtattgttgt tcagtggcag cgtgctggac caatcgttac
    37381 gcccggtgaa gtcgaaaaac agggcatttt tccaaaccag ctgtccgttg tagaacaggc
    37441 tattaatttg ctttcgggat agtgagttaa ttgaaaccac cggaccccgg ctattctcaa
    37501 gcgtgtaaac atcttcaacc gatagctcgg tggcgtagga attgagcgat tgggagtatt
    37561 gcctcatcag gttacctcca aggttaaagc ttaacgatag cttgcttgaa aggttgcggt
    37621 tgaaggagag cagaaagtcg gtattcaact ccctgaaggt cagatgcgat tcgccatagt
    37681 agcccaacgg gctgtctata tctccaaaag cggagcgggc gttgttgtag tttttaaagt
    37741 agtcaatgcc tgcaccaact ttaaggttga gccaatcggt aaatttgagg tttatacttc
    37801 cctgtccaaa cagcctgtcg cggtcaagcc cgttgaggtt ttcatacagg gtgaagtagg
    37861 ggttattatg gtagctgtag ttccaatttc gctttgaccc gtcgggaaat ctatactgcc
    37921 tcaaatcggc aacatcaacc tggcggccaa accaaagaaa ttgctgcata acgttttgtg
    37981 catcgtagcc gtaggcgggc aggttgtcgc tgtgggtgtt cacgtagcca aggctggcat
    38041 ttagggttag gtaggtatag ggcgaaatgg aggcgttgag cattacgtta ttcttcgact
    38101 ggtcggtgtt cgggaccatt cctttttgct cggtattagt gtaggagaag cgtgcagcat
    38161 agctttcatc agcaccttca actgaaacat tgttggtaaa agtcattcct gtttcaaaaa
    38221 aattctttac attgtcggga tgggaaatcc aaggagtagg ttgcctaatc ccgcctgcca
    38281 caggagagtt ccattgaggt atcattagcc caatgtcgag aggcggtccc cagctttcat
    38341 caacattgtc gttaatgcca ttacccatgc catcataaaa ttcaaacaaa ccaccagagc
    38401 cttgtccgta cttattttga aattctggca gccgcattgg cgactccatg gtgatggtgc
    38461 tgttgaacga tacgccaaaa tgatcgtcaa catcggcact cttggtctta atgattataa
    38521 ccccattggc tgcacgcgat ccgtagagtg ctgccgcatt aggccctttt agaacggtaa
    38581 tatcctcaat atcattagga ttaatatcgg caacgccact tcccctgttc accccttcgg
    38641 ttgtagtgtt tccgaaattg gcgttgctca gcaccacgtt atcaaccaca aatagaggct
    38701 ggttatcggt attgagcgag ttaattcccc taagcactat tctggatgat gcgcctactg
    38761 ccccggacgt gcttgtaata attgcgcctg ctactttccc ctgaagagca ttcagcaggc
    38821 ttgtctcgct ggtgcggtta atgtcttcac cctttacgct ttgtaaagca tagccggttg
    38881 atcgctccgc ccgcattatt cccaacgctg taacttttac ctcctgcatt tcaatggcct
    38941 cttcctcaag cgcaacgtct ataacccttc gtccggctat gtcaacctcc tgggtcttat
    39001 agccaatgca tgtaaagcgt agagttttgg agattgatgg ggttaccagc tcgtaccggc
    39061 ccaggctatc ggtaaatgtt ccccgtgccg atccaactac taccactgta acccccctta
    39121 tgggatatcc tcctgttagt gaaattactg ttccacttat ttttatgttc tgtgctgcta
    39181 ttttgcttgc gggtaatacc agtaaaataa taaaaaaaaa tgagaggaag ccgctcaatt
    39241 ttctcataga aacaaccatt ttaaatattt ctgattcgaa cctgcttaaa tattaagcta
    39301 tttggtaggc aattttatgg cgttaaacaa ctcgtgtcaa gttttttatc aaaaaaaaca
    39361 tataatctgt taccttccta ttagtctgaa tgttataaat actactggca atttttttcc
    39421 ccttgaagct gaataccaag tcaaaaccta tatatttcga ttttagagta actcctgttg
    39481 ccgctaattt ttcatttgcg gtggattgct taagaatttt aaccaacatt tatttaataa
    39541 acacaaaatg agtggttatg gaagaccggt ggatggcggc ttaagcatac aggagtgtcg
    39601 gaatagtagt tttctagtag cataatactt aggcaactta gtagaagttt gaatattagc
    39661 tggataggca gatgaaaagg ataggaatac tctccgatac gcatgggact ttagatgatg
    39721 gagtaatgga atttttctcc gatgtcgatg aaatttggca tgcgggggat attggctccc
    39781 ttagcttggc cgataggttg gccagcttta ggccattcag ggcagtttac ggcaatgttg
    39841 acgatgccag agttaggctg agctacccac tttcacagca ttttcgggtc gaagatattg
    39901 ttgtgtttat gacccatatt ggaggcaggc cgggaagata tgagccaatt gtgtatgaag
    39961 ccattaatag caatgaggtc aacttgctgg tttgtgggca tagccatata ctcctggtaa
    40021 agtttgatag caagcataac ttgttgttta ttaacccggg cgcagccggc aacaatggat
    40081 ttcacaaggt taaaacggta gttaagctgg atattgaagg taaacagatg aaaaacctgc
    40141 aggtttggga aaaaacacgc ttttagtcca gcagttgtcc taaattagcc agcgccagat
    40201 cttcttgaat ttgtcaagta gcggataagt tttgaaatcc tccaggaggc ggactagttc
    40261 tgaaatcttt aatcgccggt aggcggacaa gttcttgaat ctggaatctg aaatctgnnn
    40321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    40381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnaat ctggaatctg aaatctggaa
    40441 tctgaaatcc gccggtaggc gggcaatttc ttgaactatc ttacccttta ccctttatcc
    40501 ttgtcaacct caccagctca atgcgggttt cgtttaccct gaggatttta atgataaaac
    40561 cctcaaactc gatggtttcg tttagtttgg gtatgttctg gtgcttaaac aggataaagc
    40621 cggcaagtgt ttcgtaatcg tcggactcag gaatatcaag ctggtagttt tcgttgagat
    40681 gtttcacctc aagccgtcct gaaaaaatat actcgttgtc gctaagcttc ttctcgacaa
    40741 actcgttgcg gtcgtgctca tcatcaattt caccgaatat ttcctccatg atatcctcaa
    40801 tagtaaccag gccagctgtt ccgccaaatt catcaacaac aatggcaata cttttatgct
    40861 cctttataaa acgagttagc attttattgg ccggcatggt ttcggggaca aagtcgatgt
    40921 tgatgagctt tgattttatg ctttggggat taatgaagag aactttagaa ttaacgtagc
    40981 cgacaatatt atcaattgag ttgctgtaaa cgggtaaccg ggagtagttg gtttcggcaa
    41041 acttggccct gagctcatca acagtggcat tcacatcaat ggcttcaata tcggttctgg
    41101 gtatcataca atcgcggaga cgtacttcgg aaaaatcgag tgcgttttga aatattttca
    41161 gctcctgggt gtggtctcct tccccatcgt cggccgatga ggcctggtca accagaaaat
    41221 cgaggtcaac cttgttaaag ataggcttag agccatcgtg ctttaccttt atcctaaata
    41281 ccaacctaat aatccagtag gaaagccagg aagtgaactt gctgatgggg taaaaaataa
    41341 tgtacaggat gtaaacaggc aaagcaaaaa acctaaggaa ggcgttaggg tgcaacctaa
    41401 aaattgactt gggtagaaac tctgcggtaa ccaaaactat tatggtgctt atcagggttt
    41461 gcaccaggag taaaagggaa ggaatctgaa tcaacttacc caatgcaggg gtaagcagaa
    41521 cggcgatttc aataccgtag ataaccagcg caatgttgtt gccaaccagc atggtggcaa
    41581 tgtactgttc cttattacct gctaaaaccg ctataacgtg cgatgtaaat ccaccttttt
    41641 ttttatcgag ctccagctta agcttattgg ccgaaaggta ggctatttcc attccagcaa
    41701 aaaagaatga gaaaaaaagt gaaatgagta tgatgattat tgtggtaccc atgttttacc
    41761 tgtttactct tccggctttt cccttcgtct tcgtcttata aaatacataa ggccggctag
    41821 tagggctagt aaaaaaagta tatagctgga ggcaaaaccg tttcgaacgg tttcataaat
    41881 ccccagcgca actaggaaaa cagtgagcag tagccaaatc atttcaagta tgtaggcaaa
    41941 tcctttcaca gctatagagc taattcacca acttagtttc taggatgcta tgtatttttt
    42001 tctgcataaa gttacaaatt taagcttaaa aagcattgcc cgatgttgga caaaattacc
    42061 catcgccttt ctagtctaaa ctgaatgata aattgttaca gtatgcctcc ttgaaaaata
    42121 tggcagccga atgtttagtt ttccgagttc tactgggtaa ttcccgggaa aaaaattaca
    42181 taggtacaac aagcaacttg atactggcat tgacgaggat aaacaatgct gggtgaaaga
    42241 atatttgagg agaaatccta ataaccatgg ggtaatccag ctatctgcta ttcaagctga
    42301 atagttccat catagggttc tttaacctcc cagctgtcaa acttttcgtc ggatacaagc
    42361 cccttcccgt aaatgttgcc attcacgctg ttaattttta cattcacatc cgtataaatc
    42421 agatgtttgc cttggtccca gtagagctcc tcggtgttaa gcacctcccc tttccggttt
    42481 cgtgccacaa cgttattctt tgcatgccaa agcatttttt tttcgtagta aatggcgtat
    42541 ttagcagtca gattcgactc tacacccagg gtatcatcat aggtatatac ggtaataccc
    42601 tcgggaaatt cggtgtaggg ttcttcggaa aactggtagt agatggtaat aggggccttc
    42661 accataattt taaccacccc attttcggtg tactcaagtt ccgagttgta ggcggtaacg
    42721 ccaggtatgc ttttcttatc cgaaaagtac tggatggctt cagtatcggt cttgcagccg
    42781 aaaaagaaag tagtacccac tatggcaatg cctggcagta tgtacctgta acccttaaat
    42841 agtgtgatgc gcatagctaa agagtttacc tgctagtagt acttacgctt aaagaaccat
    42901 ggtgaatcgt gaaaggtaat gcccaggttc aggacaaaga acgactcctt aataagatag
    42961 ttttcatttg tcccccgcct accaagttcg gccgacaggt taaagctggt attattccta
    43021 aagggtaagc caaacccaag ggaaattcca tattcttcaa tctgcctaga gttgataata
    43081 aggttagtct tattgtaaaa tccccctgcc cgatagctga tttttttaag gtagctcttc
    43141 aaatcgctac ggttaggggt gaactcaacc cccaaacgga aggcttccat ttttcccatc
    43201 tgaagggtgg tgttaaaagc ttccgtttta gtccaatcct gatagtaata ttcggctgtt
    43261 gcaaagagct tatccttaaa cgtgtaggaa atacctgagt taacacctgt aggaagtttg
    43321 aatttacttt tagattgggg atgtggtatc agggaatcaa cgtaggttga cccttcgagc
    43381 ttggttagcc acttatactt catgttgata tccgaacccg catccacggt aacgccaaac
    43441 cccaacgagt tgtttttatc ctcgtctagg aaaagcctgt actggaaccc cacgttaaac
    43501 gatacatcgc tgaccataat gcttgagccc acactggcat tagtgtagga ataggacgag
    43561 gacggaaaga ttatgttgtt gttgtagtcg aggcttccaa agtagtagat gaaattagcg
    43621 ccaattgaga agtttttaaa aggagcgtaa cctgcaccaa caaacagctg gttaatgcct
    43681 cctttgccgg catgctcgta cttaattctt ccaatagtgc taagaatata tggatcggtt
    43741 tcaaaacgaa ttagcctgta gcccacgctg ctatatgggg caatgccagc agccattcca
    43801 atttttttcg acaccggaaa agcaatggca atatggtgaa tgccccctgc gctgcttgag
    43861 gccgatacct tattcaggta ctggtcataa ccgctgaacg atgaattgtt gctctccaaa
    43921 ccaaaatcaa acgtgaacga catggaatcc tgagcagcat aagaggctgg gttcaggtag
    43981 tttatcatgt aggggcttct taccgattgc gaaacgcctc ccattgccct ggtttggcta
    44041 aacccatgag ttggtataat gccaattcca aacctggagt agggcgaata ggtatagata
    44101 ttttgtgaaa aaacttctag agctgctagc gtaaagccca ctaaaagtaa aatttttcta
    44161 ctattctgca ttgagttcca aaattcggtt taacccggta tagattaaat ttgggtttac
    44221 aaatatgcta ttttttaact tatctacaaa gaagctggaa tccccacctg ttaaaactac
    44281 cctgctgttg ctataaatta ggttaaattt ctcgatatac gcgtcaacct cagcaaccac
    44341 gccatttagc acaccggaaa gtatgcagtc gtttgtgttc cgaccaatca ggctgaaggt
    44401 attggaaatg cttagtaggg gtagctttgc ggtgtagtca ttcaacgatt tgaagcgtag
    44461 cgatattccg ggtgaaatac caccacccag gtagatttcg tcgctagtca ccacatcgta
    44521 ggtaatggcc gttccacagt caacaaccag cacgttttga tttgggtaaa gggtaatagc
    44581 tcccacggca accgcaatcc ggtcgtaacc gagagtcagc ggggtttcat aggtgttttt
    44641 taaaggaagt tttgacttag acccaagcac gtgtaccata agtttttggc taaggtagct
    44701 taatatttcg ggctgaaaat ttccaacaac cgagactata gccttatggg gcaggtaggt
    44761 agataaaatt tcttccatgc tcccagcgtt gagggtggag aaacttttca gaaagacaat
    44821 ctcgccctgc tccatgaggg caacctttac ctgcgtattt ccaacatcaa taattaagtt
    44881 catacataac aggaatatat tggcggatgt gtaaatttat gggtcatttt acgggcaagg
    44941 tttcatgaag cttctgcaac gtttcaattg cctcactaat ttcacctact ccggctatac
    45001 ggattgtcag cttatccttt ccctccttga gcttaaagcg ctcgggatgt tgctggatga
    45061 aacgaattat accctcaaaa gtagaagaac ggtagtacgg cgaaagctgg ttggagatga
    45121 aatgagccat cagctggccg ttacccagta ttagcttttc aaagcccagc tcaacggcta
    45181 tccacctaag tcttacaacg cttaagagat cttccacctg ccggggcagt ggtccaaacc
    45241 tatcccgaat ttcattcctg aacctttcta gctcctcggc attatggagg ctatcaattt
    45301 cacggtagag cttaatccgc tcggtggtgt tggtgatgta gctgtcgggt agaagtattt
    45361 ccatgtcagt ttccacgtgg caatcggcat aggaaggctt ccattccctt tgggtctcat
    45421 gttcggcctg agtttgaatg gtaacaaact ccgattccct caactcctcg agtgcttcat
    45481 ccaaaatacg ctggtaggcc tcaaaaccga tttcggttat aaacccgctt tgctccgccc
    45541 caagaatgtt accagctcct cgtatatcca aatcctgcat ggcaatgcta aatccactgc
    45601 cgagttcgga aaactcctca atagccctca acctacgacg agcatctgct gttaaagttt
    45661 ccaacggtgg ggcaagcagg tagcaaaagg cctttttatt tgaacgccct accctgcccc
    45721 taagctggtg caaatcgctt agcccaaaca tctgggcatt gttaataatc atggtattgg
    45781 cattcgaaat atccaatcct gactcaatta tagaggttga tataagcaca tcgtagtcac
    45841 cgcttatgaa gcctagcata attttctcta gtttcgaggg ctccatctgg ccgtgggcta
    45901 cagcggtttt ggcttttggg caaatcttat tcaccatgag ctccacttcg gctatatttt
    45961 gaatacggtt atgcacgaag aacacctgtc cgcccctatc catttcataa tcaatggctt
    46021 cctttataat tgctgtgctg aaggtatgca cctcggttag tatggggtgg cggttggcag
    46081 gcggagtatt gattatggag agatcgcgag cacccatgag tgaaaattga agggtgcgtg
    46141 gaattggagt agcggttagg gtgagggtat caacattcag ctttagctgc ttaagctttt
    46201 cctttgctgc aacaccgaat ttttgctcct catccacaac gagtaacccc aaatctttaa
    46261 acttcagcgt gctgttgagt agggcatggg ttccaatcag aatatcaatt ttgccctcgg
    46321 atgccctttg gcgtatctcg cgttgctgtg cgggagtttt catgcgattg agaaactcaa
    46381 ctgtacaggg gaaccccttg agcctactgg tgaatgtttg gtaatgctga agggcaagaa
    46441 tggtggttgg caccagcacg gcaacctgct tgctatcggc aaccgcttta aaggctgccc
    46501 taatggcaac ttctgttttt ccaaatccta catcaccgca tatcagccta tccattggcc
    46561 tttcagcctc catgtcttcc ttaactgcct tagtggcaga aagctggtcg ggagtatcct
    46621 caaaaacaaa agaggcttca agctcctgct gcatataggt gtcgggcgaa aagctgaaac
    46681 cgggctgcga ccttcgctgg gagtacaggg caatcaactc ccgggcaata tccttgattt
    46741 ttcttttggt gttctgctta agccgctgcc atgcacccga acccagctta tagattttag
    46801 gagattcggc atcctttcca cggtactttg aaatcttatg caggttatga atgcttacca
    46861 gcagggtatc gttatcctga tacaccagcc tgatagcctc ctgctttttt ccgttaatct
    46921 cagttgaaac aagacctccg aaaattccaa ttccatggtc tatatgcacc acgtagtctc
    46981 ccggctgtag atctaccaac tcctgcatgg ttatggcatc ccgacggtta aactcgtgtt
    47041 tcagtttgta ccggtggtac ctttcaaata tctggtggtc ggtgtacagg ttaattcgga
    47101 ggttatggtc aacaaaacct tccgagatgg tcccttccac cgggttgtag gcaatgtcgg
    47161 gggcaatacc gtgcattatt ccatcgagcc gctccagctg agcgctgttg tcggacagaa
    47221 taaaagtttg atagcccctc ccggcatcct cgttgatttt ttgaacaaga agttcaaaat
    47281 ttttcccaaa tgcgggttgg ggagatgtgc caaaatcgaa ggtgggtagc tgcacggaca
    47341 acggtttaat accaaagtat agggttgacc gggtgagcag gctgttggaa aaaatgtctc
    47401 gggaagcaaa gttactggcg ttaagctggg ttgctgtgga aacaatacgg cttagctcat
    47461 ccatccggtt cagcaccaat tccggatttt ttacccagag cgtggtgctt ggaggcagca
    47521 aatcggtcag cagaatcctt gaagggttgt cctcgggcga ttgcaggttg ggaataataa
    47581 ctacttcgct gagcagctcc ttcgatagct ggtcttccac gtcaaacgac ctaatagttt
    47641 ccacctcatc accaaagaaa tcaagcctat agggagtgga agcagagtat gagaaaacat
    47701 ccacaatgct cccccttacg gagaactgtc caggttcata aacaaaatcg gaacgctgaa
    47761 agccgtactc tgtaagtgta tcgcaaatga agtcgggaga aactttttcg cccttctgga
    47821 tttttatgct gttacggctg agcgtatcca ggctcaccac cttttcaaat agggcatcgg
    47881 gataggtaac aactacaaca ggcctttcgt cattttcgtt gagagctgga aggagcgcta
    47941 caacgtcagt ccgctgaaca atagcatcgg ggagagtttg cccatactga accgaacgct
    48001 tgtaggacga tgggaaaaag tacacttgat tttcgggcag cagacacgac aaatcactaa
    48061 aaagataagc tgcttcctcc ttatcgttta ccacaaccag cgatggccgt cgcctctgct
    48121 caacgtaacc ggacagtaca aatgcaaccg cagacccggc aaggccttta aggaaaaaac
    48181 cgccctccag attctgacca gcatccaaaa gctgcttcag cgaaggatgc tttaggtaaa
    48241 gcttatatag ctcgtgttgg tgttgcaata aaaaaatttt cagcaaaagt aatcctaatt
    48301 agtcgaattg cgatatgcaa taatgctcat attcatactt taaacggaca aaggcattgc
    48361 cagcatatag cttgaagaac catcccttag ggtgcttttt aaaccctttt gctttagacg
    48421 gttataaacg attcaatcta tcccgatact gctttacaaa gccgaccaat atttttctat
    48481 ttttaccaaa attttctagg tatggaaaag ggagtagttt caaaaagtat ggcagcgtta
    48541 cgtcgcgaac caacccacac cagcgaaatg gtgaaccagc tgctttttgg agagaccttc
    48601 gacatggttg agcgccaggg tggatggtta aaagttaaag gccataccga cgggtattca
    48661 ggatggatgg acgaaaggct atcgctacag ctaccagaca actcgccaat ctttaccgac
    48721 aaacctaaaa ttgccagtcg gctcttcagg gcgaaagctg caagtggatt cacaacctac
    48781 ctttgtccag gatcgcagct gcactccttt gatggaaaaa ccttcagaaa tggcagctcc
    48841 agctttgtgt gcatggacac cccattcgcc aagttgcccg acaacccacg cgaaaaaatt
    48901 cttgcgctgt ccaccacctt catggatgcg ccctacctat ggggtggccg atcacttttc
    48961 ggagttgact gctccggcct tgtgcaggtg gttttcgaga tagccggaac tatgctgccc
    49021 cgtgatgccc gcgatcaggc tggcattggc caaaccatag aatttgcgaa tgaagcaaaa
    49081 cctggagatc tggcattttt cgacgatgaa aatggggtta tcacccatgt cggtattgtt
    49141 gccgaaaatg ccaccattat ccattgctcg ggatttgtac gtgtggacag gttcgatcac
    49201 caaggcatat ttaacctcga agcccaccgc tacacccata agctaagaat tatccgaaga
    49261 gtaatttttg attgataatc tttttccaaa ggcctatatt taccaaaaag aaatccatgc
    49321 tgaaaaatca gatagtcatc gtctttgtca tcatccttat tgcagtctgg attgccttta
    49381 aaataaggaa taagcggcgc aaccagggag atgacgatga aaataatgat gagttgtcca
    49441 tgtaataagc tgaaaaatgt actgctacga atatccccga atgctggtaa cagttgacgc
    49501 agtgatcttc aggaccgata ctcaaggcca ccctgttgac ttgctgctca tagaaaggaa
    49561 aaacgaaccc tataaaggtt gctacgcatt acccggggga tttccagaga tgaatgagct
    49621 gctttcggac gcagcagccc gtgaattaaa ggaagaaact gggcttgacg gaataacgtt
    49681 gaagcaggta ggggctttcg acagggtgga tagagatccc cgcgaccgca acatttctgt
    49741 tgcctactta ggatttgcct ctgctgatag ccctttgcca aatgctggag acgatgccgc
    49801 tactgctagc tggtttcccc ttaataatct tccaccgctg gcattcgacc atgccgaaat
    49861 acttgagaag gctctaagca acattggtat ttagctacac aatcattcca gcgccaacag
    49921 taacgtttgt ggcttcgtca ataattatca ggctgccggt tatcctgttt tgccggtatg
    49981 aatcgtagaa aagcggcttg gtggttttaa ttgtaatgca ggctatatcg ttcattccaa
    50041 tgctcttttc atggtaatcc ttctccaggg tgttgatgtt taccttgtac tttacctcct
    50101 taatcagaca cctcaggtca ttggtggtgt gcttaacggc atacttacct cccaccacga
    50161 gtggtttttc ggaaaaccag caaatcatca gatccacctc ctgcccctgc ttgatgccat
    50221 tctcctcggg aatgatgagg ccaccacgcg atatgtcaag gtcatcatcc agaagaatgg
    50281 ttccggattg cggggcggta atggagtcga ggctgtctgt ccagaagtcg atgcccttaa
    50341 tcacggagct aaaccccgag ggtagcacct tcaccctgtc tccaacccta aacgttccgc
    50401 tagccatgcg cccggcatag ccccggtaat cgggaaattc cgcagactgt gggcgaataa
    50461 catactgaac aggaaaacgg gcatccagct ggttgtagtc gttcgaaatg tagagattct
    50521 cgagcaagta catcaacgtt ggcccctgat accaatccat gtgctttgaa cgctccacca
    50581 cgttatcgcc caaaagcgcg cttatcggaa taaaccgcaa atcgtataag tcaagaaaac
    50641 ttgaaaattc caggaaccta cgctgaatgg cctcaaagga ttcctgagcg tagtccacca
    50701 tgtccatctt gttcacacat acaatcacat ggggtattct gagaagcgag gcaatgtagg
    50761 catgacgaat ggtctgctca acaataccgt gcctggcatc aaccagaatt acagcagcat
    50821 ttgcggttga tgcccctgta accatgttac gcgtgtactg agtatggcca ggggtatcgg
    50881 caatgatgaa ctttctctta ggggtatgaa agtacctgta ggccacatcg atggtaatac
    50941 cctgctccct ctcggcacgc agcccatcgg taagcagcgc caagtctaag gaatcctcac
    51001 cttttttaat gcttgcccgc tcaatagcct ccagctgatc ctgaaagata gccttggaat
    51061 catagagcag gcgcccaatc agtgtacttt tcccatcgtc aacgcttcct gccgtggtaa
    51121 aacgtagcaa ctccatgtcg aggtacgcgc tactggtaat ttcctgttcc atcaattttt
    51181 agtaaaaaga ttatcaagtt aaatattttg aaacctgtat tggcttaatc ttactgccat
    51241 gaaacaattc ttgcgaatgt aagtttccgt aaatagtaag gtgtttaaaa ataaccctct
    51301 attttcctgt cttccatggc cgcttccgag cgcttatcat cggcacgacc accgcgctcg
    51361 gtggttcggg tggcagccac ctcgcggata atctcctcaa gcgtgctggc cgcagactcc
    51421 accgcaccgg tacaggttgc atcgcctatg gttctgaacc gcacaatacg cttttcagcc
    51481 acctcgtcat ctttcatcgg caggaatggg gttttggcat agaggacccc gtcgcgcatg
    51541 aatacgtcgc gttcgtgtga aaagtacagt gagggcaggg caatctgttc cattagcacg
    51601 tactgccaaa cgtccatttc ggtccagttg cttatgggga aaactctaaa atgctcgccc
    51661 agattctttc gcccgttgaa taggttccaa agttccggcc gctggttctt ggggtcccac
    51721 tgtccaaact catcgcggtg cgaaaagaat cgctcctttg cccgtgcttt ctcctcgtcg
    51781 cgacgtcccc ctcccatggc gcaatcaacc ttcaactcct caatggcatc gagcagggta
    51841 acggtttgaa gcttgttacg gctggcattg tagccttttt cctcggcaac cctacccctg
    51901 tcaatggaat cctgaacgta acgaacaata agctgcaccc ctgtttcctt aaccagccag
    51961 tcgcgaaact ccagggtttc ggggaaatta tgacccgtat caatatgcaa aagaggaaac
    52021 ggtactttgg ccggataaaa ggccttgcgg gcaaggtgaa acatcacgat ggaatcctta
    52081 cccccactga acagcataac gggattctca aactgcgaaa caacctctcg aatcacgtaa
    52141 attgactcgg actccagctc ccgtaaatga tttagcttga atttactcat atcaacgact
    52201 tacttctcct ttaaaatttt cagcacaaat ataacaacga ttgcgcagct catgctcact
    52261 tacaacctat aaccttaaca ctcaaacaga caaaagggtt caacctacca tggaatctcc
    52321 agccagtcaa ccacatgggt aaacagcata tttaccgcct cctctacccc gattgaattg
    52381 ttgaatatga caagatttgg atcggttgga ttctcatacg ctgccgaaaa acctgtgagg
    52441 ttatttagct gtccttgttc agccttggcg taaagtcctt taacatcacg cttcatgcag
    52501 gtttcgaggc tggcatttac gaatacttcg tagaaaaacc tactgcccac aattgatctg
    52561 gccaaatccc tcatggattg aagcggtgat accaggcagc aaattgccac taatccttgg
    52621 gaattgagca gcttggcaat atgggcaacc cgccggatat tctcctttcg gtcgtcatcg
    52681 gaaaagccca ggtcggagca tacgccgctg cgaacctcat caccatccag aagcacatat
    52741 cctacgccaa tgccatcgag catccggcct aagcccaacc ctattgttga tttacccgac
    52801 gagggtagtc ctgttagcca aattgttgca ggtgtacagc tagtcataag gaaataacta
    52861 tgtaatgcta atgagtacag aagaaaaaat tccaacctac cgtaaatcta taacctctat
    52921 gcccttacgg gttgaaatat cgaatgtaaa ggtgccatcg ggagcaggga cgttggtttt
    52981 aaattgacta atgttgacga tgtagcttgt cccatccttt ccatggtatc tgataaaaac
    53041 cggttcgagc gaaaccttgt caaatctcag cttaaccgaa acgtagggag agtcaagggc
    53101 ttttgggtag aagtccattt cgaccagcgt gcggtttttc accgtggttt ccgatataaa
    53161 tctcacctta aacctggatt cataatctct aaaaatgcca gcggggtcat caagagggct
    53221 gcccaactcc ccaccggaag tggaaatggt aacctcgttg gcctcgccaa tgtactgcca
    53281 cttggttgag ccatcaaaat aaacctccat gcccataatc ttcagcaggt acttgttgcc
    53341 cttaatcaga atttcaccct gctgggtgtt ggcaacattt tcctggcggt tctcgatggt
    53401 aagtgaaaaa gtggcattta tactactggc ttccagtatt ttactgctaa aatcccaaaa
    53461 agtttgtggt acatcctggg attcctgcgc aacgaggttt accgataata gggcgaaaac
    53521 aaacagcatt aaacttctca tagcctaatt attaaacgaa atatcgccat ttagcagcca
    53581 tcaaggtgta cagctggtgt ggctcactgg ttagataaac ccctcaatat ttgttccaaa
    53641 gacagctcgt cggcaatcag cacctgccgg gccttgcttc cttcaaacgg gccaacgata
    53701 ccggctgctt cgagctggtc aactattcgt ccggctcggt tatagccaat cgagagctta
    53761 cgttgaatta gggaggtgga gccaatttgc gtttgaacca ccatgcgagc agcctcctca
    53821 aaaagctcat cacgcttatt caggtctatc tccctaaagc cgtcctgccc ttcgggtgca
    53881 tagtcgggga gctggtaggc ttcggggtag ccctgctggt taccaataaa atcggttatt
    53941 cgttcaattt cagggatatc gataaaagca cactgcaccc tcaccatgtc gcttccggca
    54001 gaaacgagca tatcgccacg gccaaccagg ttattggccc ccgggtggtc aagaattgtg
    54061 cgggaatcta tcatggaagg gactctgaag gctatacgtg ctgggaagtt ggcctttata
    54121 acgccggtaa tgatgttggt ggacggccgt tgcgtggcaa taatcaggtg aatgccaata
    54181 gctctggcca gctgggctaa acgggccagg ggtgtttcta cctcccgccc ggcagtcata
    54241 atcaaatcag caaactcatc aattatcaga atgatgtagg gcagaaaccg gtgccccttg
    54301 ttggggttca acctacgctc aacaaatttt tcgttatact ccttgatatt cctcacctgg
    54361 gccagcttca gcagctcgta gcggttatcc atctcgatgc aaagggagtt gagggtgctg
    54421 attacctttt gcgtatcggt aataatgggt tcttccgaac ctggaagttc ggccaggaaa
    54481 tgcctttcca gcttgctgta aagggtaagc tcaacctttt tggggtcaac cagcacaaac
    54541 ttgatttccg ccggatgctt tttgtaaagc aacgaggtga ttatggcgtt cagcccaacc
    54601 gatttaccct ggcctgttgc tccggcaacc agcaagtggg gtagcttggc caaatcgagc
    54661 atgaatatct cattcgatat ggtttttcct aaagctatgg ccaattcata cttgcactcc
    54721 tgaaacttct gtgatttgat gatggagtgc atcgaaacaa tttcggggtt ctgatttggc
    54781 acctcaatac ctattgttcc cttaccggga attggcgcaa taattcttat gcccagggca
    54841 gccaagctaa gggcaatatc gtcctccaga cttttaattt tgctgatgcg gattccaggt
    54901 gcaggaacaa tttcgtaaag cgtaacggtg gggcctatgg ttgcctttat cttatcaatc
    54961 tgtatcttgt agtgcgacag ggtttctaca attcggttct tattttcaat aagcgtttcg
    55021 tttgatacgg gcgaggtggt gtttttgtaa tcctccagca gctccagcag cggacgctgg
    55081 tagcgggaga gctctaaagt ggggtcgtat agctcctgtc cgttcacctc aaccgggttt
    55141 agctccacct cgttgatttt ttcaatggtg aactccacat cggatttttc gggagtattt
    55201 tctgcatttg aactgttagt gccagctgtc tcctcttcga taagctcgaa tggctcagca
    55261 tcaaccaatt ctgaattagg ctcgggaatg aacttttcca aagtaacctc aacagcggca
    55321 tcggtgttat taggtagaac ttcagagtca acttgtccag ccaactcctc agcagttttt
    55381 tcttttttat tttgcctttt atcatttttt cgggaaaccg ggcgcctcat tctaataagt
    55441 aatcccctga tggttcttct gaaccagtgt agcgttgaag gcttgataaa aatggtgtag
    55501 gcaataacca gaagtaacag caaaaaagag gttccaacct tacccagcaa ggccgacagc
    55561 catttcgaca caaaaagtcc ttgcgctccc cctagcccgc taccaagaaa tcccttagcg
    55621 gtttcaaata aaaagcccag cactagcgag catagtagtg ttcctatgaa gatattaaag
    55681 ataaaccgcc agatattttt tctgtacagc ttaagaagct taagtccaaa aactatgata
    55741 atagatggca gcgcaagcga agagaggcca aaccagtggt tcataaactg agccgatata
    55801 accgctccaa acctcccggt ccagttcttc acctcgtatt cggctttgga aaaaaggctt
    55861 tgccactcca gactctggtc aactttccac gtgaacaggt atgaaatcag ggaaataaga
    55921 aagtaaacac ccagtagcag caagaataac ccaatggtaa accttaccct ttcgtcccgg
    55981 agaagtttat ttttcccgtt gtcctgcccc tcctttgtgg cgttgttctt ttcctttaca
    56041 tttggccggt tgctatctga tgccatccct gaatcgttta tttttaaagc aagagctggg
    56101 gcaaaaggac agaattcatg actggcagaa tctccttacg gtttaagcac cctggttgca
    56161 gttgtccaac ttgcattatc ccctacatca ttaagacaag atgcaaaagt aatgaataaa
    56221 atccgacaaa atccaaaggt taatgtactt ttggggaaca acctacattc aactcttacc
    56281 ggtagattta caaacttact gctgaagcgt gtagattaaa ttggctatcg tttgtgaatc
    56341 ggtaacaaca tagtccgatg gaggagcaaa cagctcaagc gggacatgct ccgactttac
    56401 cgaccttgcg gtggcgtgca tggtaaggtt attcagcctg atgttaaact gcaacagggt
    56461 accctttacc tcctcaaagg gtgtgtgggc atttgggttt tcgatttgaa tctggctggt
    56521 gtaaataatt tcgaatggga aatcgggatt atcggcaaag tagcctttag ctttagtgca
    56581 gctataaccc aaaaattcgc atgtttcacc ctccgaaatc aactttaccg cgggcattcg
    56641 ggagtaaagt gccgggtaac gctcctgttc tatgggttcg ctgtggtaaa gttttttatt
    56701 aaaaatctta agcagtgtcg aaaattcatt acgttcagga ttcttaataa ttgaaatgga
    56761 aatggcgccc cctagagcct caatggaatt tatggaatgg ttgctgttaa acctaactat
    56821 caactcggaa ggtagcagcc atgcatcata ccggctctga attgcgctat cgtaggtgat
    56881 gtcataagta ataacaccct ctgaaatgct attgttttta accctaccgc cacatgaaca
    56941 aagaataaaa gcagacaaaa tgaccaaacc ggcaattggc ttcctcatat gcatcatcct
    57001 taacaaacca taaaagtagt aaaatagccg ccatatatac aactattgca ataataaact
    57061 aggccatgtc ccaaaaggca agctagctgg ctaatccccc ttttttcact tccagcgagt
    57121 cagcattcgg agaaaacaat ctcgaacctt tctaaaaaag aggtgatgaa aagcagcaat
    57181 aaaaaatatg ttattaaaca tactgcaaaa ctattgatta agcaagcctg aagatggcag
    57241 ggtaaaatac ctttgaaaaa ttttaatagc tttgcaattt taaatcaaca ttatattaat
    57301 aatttgtata tgagcaaatt agcagttgtt gctttaggag gtaatgccct cctaaggggg
    57361 aatcaggttg ggacaataga agaacaggag caaaacacaa ccgaaacact ggagaacctg
    57421 gtttatctga ttaaggaagg ctacaatctt gtcattagcc atggaaatgg ccctcaggtg
    57481 ggcaatatcc tcatgcggaa cgatgcggga gagcagctat acggcattcc ccaaatgccc
    57541 ctggatgtat gcgttgccga ttcgcaggga ggaattggat acatgattga acgcatgctc
    57601 cgtaacgtgc tgaaaaatca cggcattgat aaggaggttt gctgtctggt aacaccggta
    57661 attgttgaca agaacgaccc agcctttagc aacccaacca agcgtgtggg gcgaatatac
    57721 gacaaggctc aagccgatgc cttggcctca caaaaaggat ggatttttaa ggaagaggtg
    57781 aaggatacgg gtagtggttg gcgcagggta gttccatccc caaagccaaa ggggattttg
    57841 aatatcaagg ttatagacca gctggtcgat cagggaataa ttgttattgc ctcaggagga
    57901 ggtggtgtgc ctgtttacat tgatgaggag aaaaatgtga ggcctgctga ggcggttatt
    57961 gacaaggatt ccgcatcagc gctaatggct gccgaaatca aggctgacga gttctacatt
    58021 ctaaccgatg tgccatttgt gttcatcaac tacaagaaac ccaacgagaa gaagttggaa
    58081 ttcctcaacc atgccgatgc aaccagatac ctccaggagg ggatgtttgg cgagggcaac
    58141 atggcaccta aaatacgtgc ctgtttgaac ttcatcgaga atggaggcaa aatgagtgtc
    58201 ataaccgaag ccaccaagtt ggaggatatg agctacggaa ctaaaataac catggcctac
    58261 gaggactagg tatcaagcta acccggtaag cataccgttc gagctataag acttttagag
    58321 aaggcatagc cctccaggaa tgcagctgaa cgggcgaacc tgaaaccatg atttcaacag
    58381 tgcattttaa gcttatcata aattgtaaaa cagcataaaa acttaagagt atgtcattca
    58441 acctaaggaa tcggagtttt ttaaaacttc ttgattttac tccgaaagaa attcagtttc
    58501 tactcgacct atcggccgac cttaaaaagg caaagtacgc cggaaccgaa cagcagcagc
    58561 taaaggggaa aaacattgtg atcctatttg agaaggactc aacccgaacc cggtgcgcct
    58621 tcgaggtggc agcctacgat cagggtgccc atgtaaccta ccttggacct tcaggttcgc
    58681 aaatggggaa aaaggaatcc atgaaggata ccgccagggt gctgggcaga atgtacgacg
    58741 gaattgagta ccgcggctac tcccaggaaa ttgtcgaaac tcttgccaag tatgcgggtg
    58801 ttcctgtatg gaacggccta accactgagt accacccaac tcaaattctg gccgattttc
    58861 ttaccatgcg cgagcatagc agcaaaccgc tgagcaaagt taccttctgc tacatgggcg
    58921 atgccagaaa caacatggga aactcgctta tggttggggc agctaaaatg ggaatggatt
    58981 tcagggcttg tgcgcctaag gcctgctggc caaaagaaga actggtaaaa acctgcatgg
    59041 agattgccaa aaccaccgga gcccggataa cccttaccga aaaggtagaa gagggcgtta
    59101 aagggtgcga tttcctctac accgatgttt gggtttccat gggtgaagcc gattctgttt
    59161 gggacgagcg aattaagctc ttaaaaccct accaggttaa ccgcaaggcc atggagctca
    59221 ccggcaatcc aaactgcaag ttcatgcact gcctgccctc cttccacaac cgcgagactg
    59281 cagtcggcga agatattttc aagaaattcg gattagaatc catggaggtt accgatgagg
    59341 tttttgagtc ggatgcctca attgtttttg atgaggctga gaatagggtg cacaccatta
    59401 aagctgtaat ggtggcaacg cttggcagct aggcttttaa atttttttgc gggaaagcag
    59461 cttaaaaaag ctgctttttt cttgcattta cattttttta gctataattt tagttgcata
    59521 ataaccaaat ctttatgcct tatgatgatt tatgttggta acattgccta ctcaatgacg
    59581 ccagatgact tgaaagagct atttgcccct tttggaaacg tgctctcggt taaaattatt
    59641 gccgacaaaa cctccggaag atcaaagggg tacggttttg ttgaaatgga aaaggatgag
    59701 gatggccaaa aagccatcag cgctctgaac gatacccagg ttaagggtag aaacataaag
    59761 gtgaataacg cctttaggaa ggatgattca aaaacagaag gaaagcagaa ggactaacca
    59821 cgggcttaaa ggtaaagcca actcctggta agtttgaatt ccgatttttt ccatgcagtt
    59881 aacattcgat gtgctgttgg tgtggtttta ccattatgtt tatttggagt ttaaaaggaa
    59941 aatcaataat gtcttttggg ctacctgaaa aaaacctcca ccgtaaagcg gtaggcaata
    60001 taacccgaaa cagcaaattt tagcccggta cctacaagaa atcctataaa tgaacctgtt
    60061 gccgagagca gcgggttatt gcttttatcc cttgcggtga gctcaccgat gaatgcgcct
    60121 acaaatggtc caacaatgat tcctaggggt ggaaaaacaa aaagcccaac caccaatcca
    60181 atagccgagc cccatactcc ccgcttggtt ccacccattt tcctggttac ccacaccggc
    60241 attacataat cgagaataac tacaacaagc gtggctatcc ccgtccataa aagcaagctc
    60301 caatcaaaat tcacaaagtt ggtaaagtag gtcagcagta atcctaccca actcaacgga
    60361 gggccaggaa ttacgggtag aaaacaacca accaatccaa caaccacaag ggcagccgct
    60421 aaaacaatca ggacataatc tactaccata cactcattta gtttgttcta cttcaaaagt
    60481 gcttcaaaaa tttctccagg aaatcctttc agcattgaat acaaacaaag atagccctaa
    60541 atttgtataa tcaaaaccag ccatgacagc taaaacaaaa gccataagtg taaaaggtag
    60601 ggttcaaggc gtggggttca ggtatttcgt gctacgcaac gccaaaaggc taggcatcag
    60661 gggtttcgta aagaatatgc ccgatagaag cgtgtacatc gaggcgcagg gacaggagga
    60721 gaatcttaat gagctgattg gtctttgctg gaaagggcct gaacggtcca gagtggacag
    60781 cgttgaaacc aacgaggtgg taaggaaaga gtggaacgac ttttacgtgg agtagagaat
    60841 ttacaaacaa cttggtctct ctagcaaatt gcaagggtaa gaatgtagta attacaactg
    60901 tttcataacg agttatcggg taacaggcta cagcataatt atatcttggg caatcacccc
    60961 tccattaata cctgctagaa gtgatatttt tctgtagagc aaattcgatt aatctccaaa
    61021 tcaattttag gctagattgc agagtctttt atgatatcgt tagaacgtag aaggatatcc
    61081 acgtactggg ctctcaggta tgcaaaggga tcgtgcagat ttttctttgc tagcttaccc
    61141 ctgaaatcat gaagggattt ataaccctta cgatgcatcc atgcctcaat ttcacccaga
    61201 attttggtaa tttgctcaat accattcgca taaatagtgc tggtaacctg aaatgtagtt
    61261 gctccggcaa gcaacatctt tacagcatca gcacctgtca ttattcccgt tgaggagcaa
    61321 atgtctgcta ctatgttgcc ataaagcaaa ccggtaaatc gcaatggcaa acgtaggtca
    61381 tcctcattgc tcagggttat ggtctgtacc aactcctcct tttcaatatc aatgtcgggc
    61441 tcaaaaaaac gattgaacag gacgaaaccc tgaatacctg tctcatcaaa gcggcgaata
    61501 acatcaagtg ggttggtgta aaacgggcta agtttaacgc taaccgggat ggtcaccttt
    61561 tcgaggacac ttttcagcgt gtcaacttgc gattgaataa tggttttccc ctctacctca
    61621 aaatttttgg gagtagtgta gaagttcagc tctataccat caacccctgt ttgctcaatc
    61681 tccttggcat attcaaccca tgtgtcggga tacatggcat taagacttgc aatgactgga
    61741 atgctcaacg tgtccttggc cttcctaagt ttagccagat gatcggcagg gccagcgtgt
    61801 ttaagcgtgg gaaagagctt aaccatttcg gcatgccgct cgttatactc ttccagctca
    61861 ttttcaagct ccagcgcttc gagttgaatt tgctcctcga aaagcgattt gaagacaact
    61921 gctcctgcac ctgcggcctc aattgccttt aacgattcaa tgtttaacga cagagacgat
    61981 gcacccgcaa ttagcggatt tttaagctct attcccatgt agctagtttt caggtcactc
    62041 atggcatacg gttttaatgt tggaaatttt ctacttcaac cgatgatagt aaaatatttg
    62101 ccaggtcaat tgccgacttg gagaagcgaa agtcagaaaa gttctgcgca ttaagcgcaa
    62161 caaccaaacc cctatcgggc gcagtaaaaa catactgatc gccatacccg gcggcaaatg
    62221 gcaatggttt tcccgatggt aaaatatcta cccaccagtg cagcccatag tatatgttat
    62281 cgttaaccct caccctgccc gccgttgaac tctccaccca ttcgctggaa accaacctcc
    62341 taccgccata tatacccccg ttaattacca gcaaaccgat tttggccata tcgaaggtag
    62401 ttaaggacaa tcctccccaa gcaggtaggc tattccctgc atagctctcc cacaagaaat
    62461 tttcaataga caggggcttg aataaaaatt cccgaaccat ctcatcgtaa gctattccag
    62521 aggcacgtga gagcacctca gctattacct gagaggcaag gctgttgtaa gaaaatttta
    62581 cacccggtag cgtgtcaacc ggcaggtaga aaaagcgctc cacccagctc cggttttcct
    62641 caatggtagc cataaggctg ttagaggcat agttataggg gaactgccac tctttgaagt
    62701 tcaaccctgc agtctgctga agcaaatggc gcagagtgat gagctttttg gtgctatctt
    62761 tctcaaaaac ctccttatac tcggggaaat agctgtaaat aggtgaatca atagaactta
    62821 taaagccctt gtcgaggcat attcccacca ttagcgaggt aatactcttg gtaaccgagc
    62881 tgatggtatg aagtgaattc cgggtggaga atccaaaata cttttcgtaa agaatagcac
    62941 cttgccggtt taaaaccacg atgctggtaa ccttaccata gtctccattg cgaattgagg
    63001 cttcaatttc cctgatagat gacctgttaa tcgagttagg taccaccctg cccgcactag
    63061 atggttttgc cacagacaaa agcagaagca aaaaaatgaa tattcgattt aggggtttca
    63121 tccgaaaaaa ctttaaccta aagttacggc aaaaaatata aaccagacaa cccaaaccct
    63181 tttgcaataa accgctcctg acaaattttg ttcacaccgg gcagcgcgat catcgcaata
    63241 ggacataaaa aaaattcccc ggctactttt ccggggaaat agttgctcat taaactcaag
    63301 ctgctatcct aaatccttag gcagcttgct taactcggtg aaacactcat cggctgaaac
    63361 cagcaactcc tgctgtaagg ctttgaagtg ggcctttgta gcctttttgt cagtcaactt
    63421 gcccggattg ttgatttttt taatagcggt gttacggctt tccataattt ttgagataat
    63481 tccctctgcc ttgtcgcgat ttttatcgcc atgcagcgtc atataggtga agcaatcgtt
    63541 aacaacctcg ccaaccaagc tatcaacatc ctttttaaga tcttttctac ttgccatggg
    63601 tgaaactatt aatttgatga actgcaaagg tataaaaaat taaaaaaaca cccagccggg
    63661 aagggaaaga ttattgaagc caaacgattt atgctgagca agcgcttgcg gtaaagaaaa
    63721 tttaagaagc caaaaaattc aggcagataa aaattccacc agctcgtttt cgactaatgg
    63781 gtatgtttcg tctatttgcc gttgcagctc aatggtggca ctgatagcgg taatgacacg
    63841 gctataatgc tttgaatcat ccatattcct tcccttcctg tctttcaggt atttctgcaa
    63901 aacctgatag cccccgattt cgtaacccca aacttcaggg gtaatgccat caaaatactt
    63961 attcttgttg atatagattc ttcccttttg ttcgtcatag gcaatccctt caattctgtc
    64021 attttcagat tcaccttgat atttagagat ggg
//
LOCUS       DAOSHM010000009        60649 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_388_length_60649_cov_20.791712, whole genome shotgun sequence.
ACCESSION   DAOSHM010000009 DAOSHM010000000
VERSION     DAOSHM010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 60649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 60649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60649
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_388_length_60649_cov_20.791712"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            196..2100
                     /locus_tag="PK839_02555"
     CDS             196..2100
                     /locus_tag="PK839_02555"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HOG71647.1"
                     /translation="MSVGYSEESIKTLEWQDHIRKRPGMYIGKLGNGSQPDDGIYVLL
                     KEVVDNSVDEFMMGAGKRIEISVVGHTVSVRDYGRGIPQGKLMDVASKMNTGAKYDSK
                     AFKKSVGLNGVGIKAVNALSKKFIIRSIREGIAKEIEFSAGLPVREQNYASNGELTGT
                     FIEFLPDDAVFGEYAFQDEYIEQMIRNYTYLNTNLTITFNGKEFVSKNGLLDLLNENL
                     GGEPLYPIIHLTGNDIEVAITHSSGYGEDYYTFVNGQHTIQGGTHLTAFRESYVKTIR
                     EFYKKDFDPADVRMSLIAAISIKVEEPIFESQTKTKLGSKDMSPDGPSIRQFIGDFIK
                     KELDNYLHKHSETASILLQKILESEKERKAISGIKKIARERAKKASLHNKKLRDCRVH
                     YNTSDPRAEESTLFITEGDSASGSITKSRDVNTQAVFSLKGKPLNTYGLTKKIVYENE
                     EFNLLQSALDIEDDMDNLRYNRIVIATDADVDGMHIRLLLISFFLQFFPELIRQEHLF
                     ILQTPLFRVRNKKKTSYCYSSEEREKAIRELGPNPEITRFKGLGEISPEEFANFIGPD
                     MRLDPVRLTKDDQIFALLEFYMGKNTPLRQDFIINNLRIEEDIVEEDTIHFEDGNVTA
                     EASFQHDTLV"
     gene            2123..4765
                     /locus_tag="PK839_02560"
     CDS             2123..4765
                     /locus_tag="PK839_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase/topoisomerase IV subunit A"
                     /protein_id="HOG71648.1"
                     /translation="MRLDDFDADELLVGGEKNTSPEEQAKIERLTADTVKRTQLTGMY
                     KNWFLDYASYVILERAVPHLDDGLKPVQRRILHAMKRLDDGRYNKVANIIGYTMQYHP
                     HGDASIGDALVQLGQKNLLVDVQGNWGNILTGDGAAAPRYIEARLSKFANDVVFNPKT
                     TEWMLSYDGRNQEPVTLPVKFPLLLAQGVEGIAVGLASKILPHNFNELIDAAVDYLRG
                     KEFEIYPDFPTGGMADCSRYNDGLRGGSVKLRARIQKVDRKTLVITELPFGKTTSSLI
                     ESIVKASEKGKIKIKRIDDNTAGNVEILIHLPPEANPDMTIDAMYAFTDCEISISTNA
                     CVIYEDKPCFMGVKDILKASVERTKELLTRELNIRLGELEEEWHFSSLEKVFFEKRIY
                     RELEKDTETWDMVIDAIERGFDPYRKLFRRPITRDDVLKLTEKPVRKISKFDIKKADE
                     HILAVEAEMAEVGKNLANVVDYTINHYQQIKKKYGKGRERKTELRSFDSIEATSVVAA
                     NEKLYVNRSEGFVGTGLKKDEYICDCSDIDDIIVFLRNGKYVVQKVQEKAFVGKDIIH
                     VAVYRKNDRQTTYNVLYRDGLNGDIMMKRCTISGTTRDKEYDITKGTAGSQILYFNAN
                     SNGETDILKVFLKPKPRLRNLILELDFSQLAVKGRDSQGNIFTRHAIHKIQVKEKLAG
                     QVEAQKVWYDPETLRLYKEAKGNFLGEFMPGEKILIANSDGTYFVAGTEYSLRFNAPP
                     LVVEKFNPNKIYSAVYFDGEQNYFYLKRFTFEPTEKIQPFIPDDGKSYLVELNSDLYP
                     NLLVRFGGKHASHQPETIDVDAFIAAKSYRAKGKRISTLNVKKVEFTEPLQKEASAFS
                     TIAGNNNAQLEEEDDGGAAEAVGK"
     gene            5091..5861
                     /locus_tag="PK839_02565"
     CDS             5091..5861
                     /locus_tag="PK839_02565"
                     /inference="COORDINATES: protein motif:HMM:NF039835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG71649.1"
                     /translation="MKTNLVKVALMAAAFVVGFSSCDDDDPTYTVTLAVNPSNAGTVT
                     GGGDYKKGDIIQLTATANENFYFVSWTEGTNELSTEATFDYTVGDKDVTLTANFNEKK
                     IVTLGAQSNTTFPGFLSVSEGATYNFNEASSHQAAVDIFCFYEVGNDIALASPGSNIT
                     GIFGGTETDPANWTTKNTTLFNQVTTLTVAQFDTIPNGDANIQTYYSADAARKKAKLL
                     IKDNIYAFKTQNGTYGLLKVIDVSVGADGFIKFEYKTK"
     gene            5932..6825
                     /locus_tag="PK839_02570"
     CDS             5932..6825
                     /locus_tag="PK839_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG71650.1"
                     /translation="MRKLTICLIAIFLLAACSEKESVDTPPSITFQTGSYTQDGEAVP
                     LGGKLTFGITANGGSEPLTNIRIQRVAGGNVITEVDKGLFVKNEDYPYVVNAVKSDAS
                     EEVWRFMAMNSKRDSVEITRTVLLGEGVAYGPIRHFPSVKIGMQNNTEYPHFLCLSTG
                     TTYSNEEVAGNEASIDLLAFVYQTSGIWSPTLNCPQYTTAPSYYPIVGSWTTRNSTLY
                     DYNAVDNNLVSLDEFNAATNDSLLVASFKPQSSSGNCKFCFTGKIVPFKTQLGKYGLI
                     RIIHADQVAEGYMELEIKIQE"
     gene            6959..9340
                     /locus_tag="PK839_02575"
     CDS             6959..9340
                     /locus_tag="PK839_02575"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOG71651.1"
                     /translation="MKHDSTTTVWDKYSCSKGWQFQLTKAAKIVALASALMLFFPIIT
                     HAQNSITGTIKDKATGDPLAGVHVSVGQQVATTNSQGFYVLKNLKPGTHIVTASLVGY
                     KSEQQPAKIVNESITLNFFLEEDLIFLNEVTVSATRTESRIADIPGRIEMITPEKISL
                     ASYQSVDELLTLIPGVHTARSFGLFSYRSSVSMRGVSSKEQGRTLVLLDGIPMNKADG
                     GSVNWNLISTREIERIEVVKGPGSALYGGNAMGGIINVVTQKPTRPLEGSVAGSYGTY
                     NTKGVNAKLAGKLNNGFYWAADGLFRNSDGYITQSIADRLTNPYIVKSTFEEKVLHLR
                     TGYDNGQKLQADVDFTLFDDVRTTGEKVYQPQGNTREYDTYQLRANVKGVAGAVVWNV
                     SAFFLNEDYKRVSEWMKDDYTWYDVLSVRRDYGLLTSANYRAGRHTFTGGVDLRMGSV
                     DASDVYYTSTDRVDNNGKMNFYGFYLQDVVALFDSTVNVTAGLRYDYARFFDGQFQIL
                     SPSAETAFMSQYQFSQNGSESWGALSPRISAQFKPNGNYRIYASYSRGFRPSVLDDLC
                     RSGRVKGGFKVANPNLKPEYLDNFEVGGDYKPFDRLRLAASAYYSTGKDFLYYVSTGD
                     SIDMGFGPRPIMIPSNISDVEIYGMEAEFSATPWRFLTLSGGYAYALSKITGYKPIAA
                     NDFDLTGKYLTDVPKHSFNAGAFLRSHIVNAGVTANYTGRMYINDQNAYDDIVLSNQL
                     PAAFTLDLKISREFFGHVNISLNIQNILDKKIYESSMSVGPGRFMMLEVKANL"
     gene            9395..10459
                     /locus_tag="PK839_02580"
     CDS             9395..10459
                     /locus_tag="PK839_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG71652.1"
                     /translation="MKRIILAASALFMASLLPSCGNHPDGISGATKDYDSNGNSFYHR
                     TTETKLKTCDLRVEGEVRKPGEINLKHYFKREVVYKQAVLNDSGQVAFTGAYRYRGYS
                     LFDLLNPFVLEKKNAKEFVPATDVYIIIENNSGDRVVFSWAEIFLGHNMHQVLIATEQ
                     ADVEPYKVKVSYPKDSVWKVVAANDLFAYRELKNPSRIIVKSFDRKYYEINRELKDPF
                     SPTVNLVVDDSLMGVIDTLNAVAPHARYHSVFYGMGMGYHGTPTFEGPLLRPLVENFL
                     AKDGAKWMRTGIACVVGKDGFRNIFSVSELFNRVDQVEPILAIPENPKKSGYYRLYHP
                     SAFYADFSVRNLSEIYLFRE"
     gene            complement(10526..11455)
                     /gene="miaA"
                     /locus_tag="PK839_02585"
     CDS             complement(10526..11455)
                     /gene="miaA"
                     /locus_tag="PK839_02585"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009291682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOG71653.1"
                     /translation="MDNCPYDIIAIVGPTASGKTLLAANLASRVNGEIISADSRQVYR
                     GMNLGTGKDYEDYVVNGKTIPYHLVDLVDAGYHYNVYEYQSDFLKVFRSLKARKVLPI
                     LCGGSGLYIEAVLRGYRLLPVPQNATLRGELEAKSNDELAKILASYKTLHNTTDLDTR
                     KRTIRAIEIEEYYLNAPTEIEPFPQLKSAIFGVRYNRATEMERIHQRLLQRLGNGMVD
                     EVRGLMESGLSFDDMAYYGLEYRYIAEHIMGKLTYDEMVSRLNIAIRQFAKRQMTWFR
                     GMERRGLSITWINGELPLEDKLNKIMEAIQNGF"
     gene            11575..11808
                     /locus_tag="PK839_02590"
     CDS             11575..11808
                     /locus_tag="PK839_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG71654.1"
                     /translation="MAALRRNNRQSRHEELNRLIWMGIVIAVTFIFVIAVATDMFRII
                     PLKRTNIVMLVLALLIVAVYVYSWIKFYRKRKQ"
     gene            11821..12270
                     /gene="smpB"
                     /locus_tag="PK839_02595"
     CDS             11821..12270
                     /gene="smpB"
                     /locus_tag="PK839_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HOG71655.1"
                     /translation="MSNEIVVKNKKATFQFEIVEKFTAGIVLKGTEIKSIRMGRASFV
                     DSYCFFIGDELWVKGLHIAEYAWGTYNNHDPKQDRKLLLTRKELTKLKRRSQEKGLTI
                     IALKLFINERGLAKVDIALARGKQLHDKRDDLREKDVRRELDRARKR"
     gene            12408..13007
                     /locus_tag="PK839_02600"
     CDS             12408..13007
                     /locus_tag="PK839_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HOG71656.1"
                     /translation="MGKVKLTVLGISYSQTQSGAYALVLSEEDGNRRIPIIIGGYEAQ
                     AIAIQLEGLTPPRPLTHDLFMNFAKAYHIEILDVNIYKLEEGVFFSKLHCTNGDKEIF
                     VDSRTSDAVALALRFDCPIYTTEEIIEKAGLTLEIENIDEDAAKEDEGEKGNAPVDSF
                     MEQLAKLSVSELKTMLDEAVRNEDYEKASKIRDELKNRS"
     gene            complement(13090..14331)
                     /locus_tag="PK839_02605"
     CDS             complement(13090..14331)
                     /locus_tag="PK839_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018028850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015506 - nucleoside:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HOG71657.1"
                     /translation="MKLKLRLTLMSFLQYAIWGSWLISLGAYLGGGLHFSGFQIGSFF
                     ATMGIASLFMPAIMGIIADRWIPAQKVLGICHILAAAFLIAAAPQTQYGTLYPLVLLS
                     VMFYMPTIALSNSVAYNALSLNGYDTVKAFPPIRVWGTVGFICAMILVDLLGFATTSY
                     QFYLSAGLGLILGIYSFTLPNCEVNKNVEKKSLVDSLGLRAFTLFKERKMAVFFIFAM
                     LLGMSLQITNAYANDYLTNYFGKISEYQGTFGVKHANILISLSQISETLCILLIPFFL
                     SRFGIKKVMLISMFAWVLRFGLLGLGNPGSGVWMLILSMIVYGVAFDFFNISGSLFVE
                     NETDAKIRSSAQGVFMIMTNGFGAFIGSYIAGLVVDATGWPDAWFVFSAYSLVVAIVF
                     AFVFKYKHNPKNSSTVSKAAQ"
     gene            14873..15943
                     /gene="lpxK"
                     /locus_tag="PK839_02610"
     CDS             14873..15943
                     /gene="lpxK"
                     /locus_tag="PK839_02610"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HOG71658.1"
                     /translation="MRQIRLLLLPIAWLYWCGVRIRNILFDAGILKGFSPDIPTICVG
                     NLAVGGTGKTPMTAYLSSLLHGNFNVAVLSRGYKRKTKGFNIVSTESTVHEVGDEPLL
                     LKLRQPSAIVAVDEDRMNGMVELFYAYPELDAIILDDAFQHRWIKPGLNLLLTEYENL
                     YTHDFPLPAGRLRDSLSERKRANLIIVTKCPENLTADRCREISAELKPNPTQIIYFTS
                     MEYGMPQPVFSESAGKLKLTDMQPVFAMTGIANPKPFVDYLQKHTALLAAKAFADHHY
                     YSEDEIEAIFTELARSTSQAVVVTTEKDAVRLLKLKLPEEIKKRIYYIPVSVKFLFNS
                     SEEFNNQILSYVRENKSDSSFH"
     gene            15906..16715
                     /locus_tag="PK839_02615"
     CDS             15906..16715
                     /locus_tag="PK839_02615"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019129299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HOG71659.1"
                     /translation="MLEKINQTVAFIKGKVTIEPEVGIILGTGLGGLVKEIEKQQALD
                     YKDIPNFPISTVEGHTGRLIFGELGGKKVVAMQGRFHFYEGYDMKQVTFPVRVMKFLG
                     IQYLFVSNASGGMNPAFEIGDLMIINDHINLMPNPLLGPNISELGPRFPDMHRAYDEK
                     LIALAKKVAASNGTAIKEGCYVGTTGPTFETPKEYQYFRIIGGDAVGMSTVPEVIVAR
                     HMNIPVFAISIITDLGVPGKIIEVSHEDVQKIGNLSEQKMTRIMKDMLTQL"
     gene            complement(16792..17319)
                     /locus_tag="PK839_02620"
     CDS             complement(16792..17319)
                     /locus_tag="PK839_02620"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF010555.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HOG71660.1"
                     /translation="MKIFLIGFMASGKTTIGRELASALQYKFIDLDDFIESRHNQTIK
                     QLFEFKGEDYFRKVENEALKEVCDYKDDYVISAGGGTSCFYNNIDFMNKNGITVYLRM
                     EVATLVSRLIESKIDRPLLWGKTGDELANYILRVLDERKKFYNKAQLVVDGDNLNPAE
                     LAQLISAIVKEEKLL"
     gene            17711..20038
                     /locus_tag="PK839_02625"
     CDS             17711..20038
                     /locus_tag="PK839_02625"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOG71661.1"
                     /translation="MKVKMRIGVKMLLFVMSTSVLVMLVIGLFIGFRINKIARDDAVR
                     IVQSEAQKTASQVKSELELDMGFSRALAHALYIYPKFDTLTLDSIFINILKNQVANNP
                     RYLTVWYSIEYSAYRPGYTKNYGRRSVTAYLRDGNIGVDIEHKNVTGDVTTSNYYSSK
                     TCNCEMLLDPYTFVYGGREVLATSLSVPVKVGDRFAGLGGVDISLEKFQETIEKIKPY
                     SNTRVSLIASDGTIIADTNPENARLKFSELFPSEEATYQLSQKIKNGLDFQYLTIQDN
                     REFLNIITPVKVGDSPTYWSVCLSIPLDVVVAEARKAVIYTILVFLFGIILQTLAIWY
                     ISNYITRPIKKTTGVLNNLAEGDIDINSKIYIHTGDELEEMARSTSKLIDGLNHTVQF
                     AREIGQGKLDSEFTLLSSKDRLGEALIDMQKSLLKAREAEVQRKDEENKQNWATRGVA
                     MFGEVLRQHNDNLNELSYLIVKNLVSYTGSIQGGIYLINDNDSDNPFLEMTACYAYDR
                     RKFMEKNILPNEGLVGRCYVEGKTILMTDVPKNYIKITSGLGGENPRCLLIVPLKVND
                     TVLGVIELASFNPYQKYQVDFIEKIAESIAATLSSVRVNIRTAELLERTQQQAEEMKA
                     QEEEMRQNMEELHATQEEMERKRLEQESINTQLAEDRTILNSLMQNLPDCMFQKDVNG
                     RYVRLSNSMLNLLGIDQPDEVIGLTDFDLFPTSLAKLLSKLDSEVLSSRQPITSKPIE
                     LVLKNGVEQDALLTLMPLVEENGDAIGVMGVMKLA"
     gene            complement(20244..21251)
                     /locus_tag="PK839_02630"
     CDS             complement(20244..21251)
                     /locus_tag="PK839_02630"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004330153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HOG71662.1"
                     /translation="MVKITAAITGVGGYVPDYILTNDELSRMVDTTDEWIMTRIGIKE
                     RRILKGEGLGTSEMGVMAVNELLQKTNTRPEEVDMLICGVVTPDMQFPATANIISDKC
                     GIKNSFGYDINAGCSSFLYSLVTASKFIESGTHKKVVVVGADKMSSITDYTDRATCPI
                     FGDGAGAVLLEPNYDGLGLLDAKMQVDGSGRKHLHQVAGGSCYPASFETVAKRQHYIY
                     QEGQPVFKAAVSNMADVSVEIMERNNIKPENLAWLVPHQANMRIIEATAHRMGISKDQ
                     VMINIQRYGNTTSATIPLCLWEWESQLKKGDNIIIATFGAGFTWGAAYIKWAYDGNSK
                     H"
     gene            complement(21245..22195)
                     /locus_tag="PK839_02635"
     CDS             complement(21245..22195)
                     /locus_tag="PK839_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014774704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate--acyl-ACP acyltransferase"
                     /protein_id="HOG71663.1"
                     /translation="MRIGVDAMGGDFAPEAVVRGSILACKQLKADERIVLFGDKAKIE
                     ELINSLEGGSVNCFDIEPCSEVIAMGDNPSKSFSKKTDSSIVRGFSALAAGAIDGFAS
                     AGSTGAMMVGAMLVVKQIPGVIRPAIAGFIPVSDNSVNLLLDVGLNPDCRPDVLYQYG
                     ILGSIYSKQVLGVENPRIALLNVGEEDEKGNLVTKSAFEQMKDSADFNFVGNIEGHNL
                     FDADKADVVVCDGFVGNIVLKEAEAFYHLLKKLSPNSSFTERFNSENYGGTPVLGINK
                     PVVIAHGASSPKAIMNMILQTREVISSNLCEKIKQAFNKW"
     gene            complement(22228..22413)
                     /gene="rpmF"
                     /locus_tag="PK839_02640"
     CDS             complement(22228..22413)
                     /gene="rpmF"
                     /locus_tag="PK839_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HOG71664.1"
                     /translation="MAHPKSKISKQRRNKRRTHYKAELPTLSTCSNCGAAVEYHRVCP
                     ECGYYRGKLAIEKSVSA"
     gene            complement(22447..22971)
                     /locus_tag="PK839_02645"
     CDS             complement(22447..22971)
                     /locus_tag="PK839_02645"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HOG71665.1"
                     /translation="MANVLDAYSINFKGLKLGKHLFSFQLGRSFFSAFEEGEIHNGEL
                     VARVSLNKQSRLLEFSTHIEGMVEIACDRCLEQFNFPVEFDGKLIVKIDASQESDSDE
                     IIYLSENDFEVNLAQYLYESIYLSMPIERYHGMNGTSASDCDSDMVSLLGSHGHENQE
                     VDPRWDKLKSLTSN"
     gene            complement(23086..24888)
                     /gene="typA"
                     /locus_tag="PK839_02650"
     CDS             complement(23086..24888)
                     /gene="typA"
                     /locus_tag="PK839_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005675329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HOG71666.1"
                     /translation="MQKIRNIAIIAHVDHGKTTLVDKMIFQGKLFKDYERPGELILDS
                     NDLERERGITILAKNLSVNYKGYKINIIDTPGHSDFGGEVERVLNMADGVLLLVDAFE
                     GTMPQTRFVLQKALAIGLKPIVVINKVDKPNCRPAEVQEQVFELMYNLDATEEQLNFP
                     TIYGSAKQGWMSNDWQKPSKDITPLLDCIIDYVPAPKVTEGTTQLLVTSLDYSSYVGR
                     IAIGKLHRGSLQAGQPISLVKRNGEVVRTKIKDLLVFEGLEKNSVERVECGDICALVG
                     IEGFDIGDTIADYENPEPLPTIAIDEPTMSMLFTINNSPFFGRDGKFVTSRHLKERLD
                     RELEKNLALRVEKTDSADSFMVFGRGVLHLSVLIETMRREGYELQVGQPKVIIKMVGG
                     EKCEPIEYLTIDLPEDVSGKAIEQVTRRKGALLLMERRGDRIHLEFEIPSRGIIGLRS
                     ILLTATAGEAVIAHRVKGFEPYRGDFETRTNGSLVAMESGISYAYALNKLQDRGRFFI
                     DDHEEVYAGQVVGEHNRSGDLVVNVTKSKKLTNMRAAGSDEKIFLAPPIRFSLEEALE
                     YIQEDEYVEVTPKAMRLRKIILDETQRKRIARVA"
     gene            25357..>26078
                     /locus_tag="PK839_02655"
     CDS             25357..>26078
                     /locus_tag="PK839_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG71667.1"
                     /translation="MKTKLLCFILLLAVIPVLGQVTPQAPQKFSYQAVLRNADGTVIG
                     NQSVEVQVKLHAQAADGAVVYTQTQTVETNQFGLVAFAVGGSSGFETIPWTDPIFMEI
                     LVKRSGESSFSSMGTTQIISVPYALVANNGIQGPYIQGQTVFSNGGGWVGTNRVMVAD
                     SSVVVTTPLGHDSSKPIFAVTNANGEMVMAVYESGVRFYVEEETTKGVKGGFAVGGLT
                     NQVKETKSGEPIYYMNIQPTQV"
     assembly_gap    26079..26178
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <26179..27059
                     /locus_tag="PK839_02660"
     CDS             <26179..27059
                     /locus_tag="PK839_02660"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fibrobacter succinogenes major paralogous
                     domain-containing protein"
                     /protein_id="HOG71668.1"
                     /translation="TKSGEPIYYMNIQPTQVQFLVEEPAVKGVKGGFAVGGLTNQGKT
                     LTPTENYFEVNRDSAQFLTPSLVSQGNITTQGNITVSGNITTNVGQADSTLTDVDGNT
                     YQTVRIGFQQWMAENLKTTHYSDGTPIDTAVHVYNDWAYPDSINIYGRLYSGLAISSG
                     NNVCPDGWHVPTPDDWNELFTFIAGPAGISDMQSRVLVLKKLLEPGTMMEGNGYWNYP
                     IYPTNETGFTARPAGQRDYLTYYSELHDYAYFWSYVVGVGLQQVSMTTMDGGNLEIMP
                     PSDPNYSFSVRCLQNP"
     gene            27491..28753
                     /locus_tag="PK839_02665"
     CDS             27491..28753
                     /locus_tag="PK839_02665"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005467817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOG71669.1"
                     /translation="MNKKKVIIAGGGFAGIQLARKLDQRLFDVLLVDKINHHQFQPLF
                     YQVATSQLEPSSISFPLRNIFSRKKNVQIRLAEILSIDRNESKIITSIGEFSYDYLVL
                     AMGCTNNFFGNENIKKNAFTLKTTYDAITIRNHIILTFEKIVSAKEDELEALNNLAIV
                     GAGPTGVELAGAFAEIKKNILPKDYAGVDFSKFNIILIEGTRNTLGSMSDASKVESRR
                     YLQQMGVTILTETFVADYDGYLLKLSNGETIKTKTVIWVAGVTGNNIEGLPEAARAPG
                     NRISVDRFNRVNGCDNVFAVGDIAYMVTPKYPKGHPQVANVAINQAKLLAKNLKSIQT
                     GKKLKAYEYKNLGTMATIGRNKAVVDLPHIHLKGYFAWFIWMSLHLMLILSVRNKLII
                     FINWAWSYLTKDTSLRLILKEDKPRTNT"
     gene            29115..31469
                     /gene="topA"
                     /locus_tag="PK839_02670"
     CDS             29115..31469
                     /gene="topA"
                     /locus_tag="PK839_02670"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HOG71670.1"
                     /translation="MIQNLVIVESPAKAKTIEKFLGKEYVVKSSFGHIRDLSKKNLGV
                     DIAKNYAPEYIVSDDKKKVVDELKKATSEAQMVWLASDEDREGEAIAWHLAEVLNLNP
                     KKTKRIVFHEITKSAILNAIETPRSIDLDLVNSQQARRVLDRLVGFELSPILWKKVKP
                     ALSAGRVQSVAVRLIVEREREIIAFTPTPYYRVTAIFMADSEFNAELSVRLSTFEEAL
                     SLLEHCKNATFTVQDVTKKPSKKAPAPPFTTSTLQQEASRKYGFSVAQTMSIAQKLYE
                     AGHITYMRTDSVNLSSTAIAAARDVIVAEYGEKYHQPRQYKTKTKGAQEAHEAIRPTY
                     LDRVKISGTSTEQKLYDLIRKRTIASQMSDAQIEKTTINIDISGSHYKFVASGEVIVF
                     DGFLKVYLESSDDEAEENGREILPPVKAGQTLAVMEIVANERFTQRPPRYTEASLVKK
                     LEELGIGRPSTYAPTISTIISRGYVAKEDRPGVERNYKSIILKNGKITRQEKVENSGA
                     EKAKLFPSDIGMVVTDFLLEYFRDILSYDFTAHIEEKFDEVALGKLEWTRMIDEFYKP
                     FHSLVEKTIKESDYTKGERVLGIDPVSGKPVSVRIGRYGPVVQLGEADASKKTKPQFA
                     SLLKGQLIETITLEEALSLFKLPRSLGNYENSEVVVGVGRFGPYVRHQSKFYSLKKDD
                     NPLTIDLNRAVELIEEGRKKERERVIKKFESPQVVEILNGRWGPYISFNKQNYKIPKD
                     KDPKLLTFDDCMKLIDEQAEKGSKRNSTAKKTLPKRQGKKGNTK"
     gene            31506..32678
                     /locus_tag="PK839_02675"
     CDS             31506..32678
                     /locus_tag="PK839_02675"
                     /inference="COORDINATES: protein motif:HMM:NF012703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HOG71671.1"
                     /translation="MDELLSFLTPTAIKGVNFSSQNSRMLQQQVVPLNKTNYKHLKKN
                     DIVVIGVNESRNSPNPSIKNSPETIRKYLYGLSTIKLAGKIYDAGDLVETSTPLKTYE
                     ALNRVVEILFQTGAATVVLGGTQELTYCLYEGVKSNKSLVTISIIDCLIDADEDLSDF
                     HSRNYVNRLLGEKLRSLFELSIVGYQGYYVGGDMVNLFQKNNHELLRLGYVRSSYREV
                     EPTLRNADIVSFDMEAIRACDSPANTIPSPNGLYAEEACQLAHYAGVGERASLFGIFN
                     YNPDTDNTEQSALLAAQLAWHFIEGYSKRKNVPKTLNTPDYLKFIVGTGTSGVDMVFY
                     CDEALDRWWMEIPISPEMGIPTNRIIIPCSHSDYTLASRQELPDRWLRIFQKIQQL"
     gene            32918..33862
                     /locus_tag="PK839_02680"
     CDS             32918..33862
                     /locus_tag="PK839_02680"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HOG71672.1"
                     /translation="MHSSSRKFLLFQLFLPFAGILMAQQDPQFSQILYNPMSINPGYA
                     GSQEMIGIGAFNKLQWTGFGDGAPVSTTVSINAPIAPFGFKSGLGLSILNDQYGFNSD
                     LGISLAYAARFKPKLINGSLGIGVSGGFVNNTIDPKWNYPVAGNDQAIPQQKESSVNL
                     DLGLGLFYTTDYMFFGVSATHINRAKINKSPIPARYTPQYYISGGYIVMFPSKTWQFE
                     PSVLIVTDIKSSRLSLNSIVRYNKKFWGGVSYRINEAVTGMIGFELFNGFRIGYAYDF
                     SLSRISKYNDGSHEFMLGYSFQLKKEKPPQQYKSIRFL"
     gene            33918..35246
                     /locus_tag="PK839_02685"
     CDS             33918..35246
                     /locus_tag="PK839_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005633367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOG71673.1"
                     /translation="MKKLLPIGIAVILLSSCSPGGNGELTGVPGRRGFSEPTPYGMVF
                     IPMGSFTIGSNDQDVAWAINSPTKTVSVDAFWMDQTEITNNQYRQFVYYVRDSIARRM
                     LAEVNDEFVITEDEFGNPIDPPVLNWEVPIDPNDEEQMEVLNNMYYSKEDAFYGRKEI
                     DTRKLFYEYYWIDMKQAGQKNNRYNFETLSYDKGEVVNSLGEKVKVDNRQSFIMRDMV
                     NVYPDTLCWISDFSYAFNEPYANSYFWHPAFDNYPVVGVTWKQATAFCIWRTQLLNKY
                     LAPEGITIQDYRLPSEAEWEYAARGDLDHSMYPWGGPYTRNIEGCFIANFKPLRGNYI
                     DDGGLIPIAAGSYEPNDYGLYDMAGNVAEWTANAFDESTYSFMHDMNPDYKYNAKPND
                     LPAMKRKVIRGGSWKDISFFLQVGSRAFEYQDSAKSYIGFRCVRSYLGAK"
     gene            35287..36573
                     /gene="gldL"
                     /locus_tag="PK839_02690"
     CDS             35287..36573
                     /gene="gldL"
                     /locus_tag="PK839_02690"
                     /inference="COORDINATES: protein motif:HMM:TIGR03513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldL"
                     /protein_id="HOG71674.1"
                     /translation="MKINIEELVTSRKWKTFMKYLYGWGASVVILGALFKILHLPGAG
                     TMLMAGMCTESIIFFFSAFEPLPDEFDWTLVFPELTGMTEEDEIDGYKSSRRQGIGAE
                     EIHEIVTGILASMPAGSVPAAQAIAPEKVETTPPHQAAATAAPQISVQHAGAGAGGAL
                     IFTEKFNQMLENAEISPALFDKVSQGLNKLSQASSSIADITTTAVHVKEFTDNMAKAS
                     ESVKTFHENYSQNSQVLNESMNHLSDSIQKTAGEMSESGKNFMDGVAGSVQNLENELT
                     AAGQKVGGRIVESCETVAGKLNMAAEGLANAYQQTAEGVAITYQQLAEAMKNNGSTIT
                     KGSTTYNQQLEQLNKNMAALNTVHEMHLQETSQRLKEAEKLYQGVDGMIKKLNVTVDE
                     SEKFKSALETLNSNITSLNNVYGNMLAAINSISTSK"
     gene            36607..38244
                     /gene="gldM"
                     /locus_tag="PK839_02695"
     CDS             36607..38244
                     /gene="gldM"
                     /locus_tag="PK839_02695"
                     /inference="COORDINATES: protein motif:HMM:TIGR03517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldM"
                     /protein_id="HOG71675.1"
                     /translation="MAHGKETPRQKMIGMMYLVLTAMLALNVSTDVLEAFVLVDNGLS
                     RTTENYRLKNDKLYSEFDKAMLVNPVKVGPWKSKADEIKEMAREIYDYVQDLKIEVVK
                     TSEGDDTPAINGREVISELIEDKANLDIGGQILIGMEGNGKAFELKRKIIKFREKAIS
                     LLDPVRGEEMIKSINSILNTDDPPVREDGTHNSWESSRFEHIPLISVIPQLTKVQVDI
                     LNTEAEVVNYLLQQIGASDFKFNVLSATVIPNSNVVFQGSDFKAEVFLAASDTTQKPK
                     IYLCRYDSTQNPVTKEWTYNPIGPQEEIPVSQNGRGLYSRRAGAIGQTSWSGLIEMKA
                     PDGSIVRKPFKHTYSVLQPNVVIAPTKMNVLYLGVDNPVDISIPGVPPDKVTASISKG
                     SIRPEGKGSYIVQPGAGSSTCEITVYANLEGRRQSMGTKTFRIKKVPPPLPRIIGITG
                     KYAAKNQLAASLGIAAEMPQDFDFDLKYKIVSFRLSATVGGFLQEKESNGSRFTDEQK
                     RIVSNLRSGSQLAITDIKAVGPSGDVIDLNDMVLKIR"
     gene            38337..39218
                     /gene="gldN"
                     /locus_tag="PK839_02700"
     CDS             38337..39218
                     /gene="gldN"
                     /locus_tag="PK839_02700"
                     /inference="COORDINATES: protein motif:HMM:TIGR03523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldN"
                     /protein_id="HOG71676.1"
                     /translation="MKRIVVLFGGFFLILGTLHAPAQDRKESLVNDGFYTRETIQQRK
                     PIPYQYVRESDVLWSKRIWRMVDLRQKMNYPLYFPTTPMQDRESLVQRLVRAIKYNEI
                     TAYDPVDDEFTTRMSYEQVIKNLGAEDRVEKQIDSTGKETDVTIKGEIRWNSIKELLI
                     KEDWFFDKQTSTMQVRIVGICPIMHDMRILQTGEEDQEETGEITKKQTFWVYFPEART
                     ILANTDFFNSFNDAQRISFDDLFFKRQFDSYIIRESNVFDNRSISDYTFGGVQTMQES
                     EKIKTDIFNMEHDLWEY"
     gene            complement(39299..40390)
                     /locus_tag="PK839_02705"
     CDS             complement(39299..40390)
                     /locus_tag="PK839_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007763404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HOG71677.1"
                     /translation="MSNKNYYCIVMAGGIGSRFWPLSRTACPKQFIDILGVGKSLLQQ
                     TFERMVKICPVENIFIVASNSYKNLILEQLPEVKESQLLLEPMRRNTAPCIAYATYKI
                     RKRNPEATVIVSPSDHLILNEQVFTNTVNLALDFASQNNALLTLGVKPNRPETGYGYI
                     QVGKPVKGINGLYRVKTFTEKPNLEMAKVFLSSGEFFWNSGLFIWNLNAITQALEEHL
                     PEVNSLFSEVASLLDSADETAAIEKTYAECKNISIDYGVMEKAENVFVICSEFGWSDL
                     GTWGSLYTHLPVNQNGNAVIARNVLLYDTSNSLISAPDGKLAVVQGLDDYIVVDTSDV
                     LLICRKQEEQQIKNILNDVAMLKDGEIYT"
     gene            40717..41460
                     /locus_tag="PK839_02710"
     CDS             40717..41460
                     /locus_tag="PK839_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG71678.1"
                     /translation="MVRIFGLIGEYFLLLKKVFSKPEKGRIYLKQTIKEMDKLGIQSI
                     GIVTIISVFMGAVITLQTAYNMENPFIPRYMIGLGTRDSMFLEFSSTIIGLILAGKVG
                     SSIASELGTMRVTEQIDALEIMGVNSASYLILPKIIATLIFNPFLCMLSMVVGIAGGW
                     MVGEFSGVISTQAYIYGIRYAFIPFYITYSLIKTVVFAFLITTISAFYGYRVQGGSLE
                     VGQASTNAVVVSSVFILLFNLILTQLLLS"
     gene            41457..42206
                     /locus_tag="PK839_02715"
     CDS             41457..42206
                     /locus_tag="PK839_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOG71679.1"
                     /translation="MITVKNLSKSFDGRMVIDNITATFEKGKTNMVIGQSGSGKTVLL
                     KCIVGLLDVDEGEIYYNDIQFNALSFKKKKEIRQKIGMLFQGAALFDSQTVEENVMFP
                     LNMFTNMAIAEKKERVAFCLKRVDIVGANKLYPAEISGGMKKRVAIARAIVLNPEFLF
                     CDEPNSGLDPKTAVVIDHLIKEITEEYGITTIINTHDMNSVMGCGDKVMFIYNGKKWW
                     EGTRFDVLDSDNQELNDFVFASALTRKIKGM"
     gene            complement(42214..42912)
                     /locus_tag="PK839_02720"
     CDS             complement(42214..42912)
                     /locus_tag="PK839_02720"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOG71680.1"
                     /translation="MKAEVCPGTECLTCGFRSEVFASLPDYQLEELSRSKVLMHAKKG
                     ETIARQNDSIEGFIFLRDGLAKLCRTNAEGREQIVGIATPNDFVGLLSLFSSKTYNYS
                     IVAIEDSSYCSVEYDMVMNLVGSNGEFARNLLAKISRVSDLLIGSRLDIDLLQLRGRV
                     AYILCFFANEIYHSTRFNLPVSRREIAELIDMRVENVVRILSEFRRDRIILIEGTYIE
                     ILDEEKLSWAKMHG"
     gene            43328..44557
                     /locus_tag="PK839_02725"
     CDS             43328..44557
                     /locus_tag="PK839_02725"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HOG71681.1"
                     /translation="MKRIVILGAGTGGTIMANKLRKALDREQWEITIVDKDPVHYYQP
                     GLLFIPFGIYGKQDVVKPKASFIPAGVNFIINEIERVDPAKNRVYLANGQTLDYDFMI
                     VSTGTSIRPEETPGLLGNEWGKSIFDFYTFNGAVALQNFLKSWEGGNLVLNIAEMPYK
                     CPVAPLEFVFLADEFFVKKGIRDKVNIFYSTPLSGAFTKPKATKMLGDLLQEKNINLI
                     PDFYLERVDAENKKIISYDGRELPFDLLTIIPVNKGADFIERSSMGDDLNYISTDKYT
                     LRSEKWSNIFVLGDASNLPTSKAGSVAHFSSDVVFDNLISSIEGRPLMAKFDGHSNCY
                     IETGFGKGSLIDFNYDTEPLPGTYPVPGIGPFGLLKNTRINHYGKVMFRWMYWHLLLK
                     GKELPVDSQMSLIGKEL"
     gene            44569..45243
                     /locus_tag="PK839_02730"
     CDS             44569..45243
                     /locus_tag="PK839_02730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG71682.1"
                     /translation="MDERIIEQQIDALNQKLDTVIELLVSQHQKNEVVEDLIDDLSIV
                     GKNAFQATVDELAVQNISIEADDLKYLFFKVLGNIKTFGELMDMLESVVDLVKDLGPI
                     VHDASIAVTEKLGQLDREGYFSYLNQLGLLAIEFKKNFTEKDITRLREEMPAVGAIVR
                     NLSQPQVVNALATITETLSEMKLDDKLDNKSLFRLFRELNKPDVRKTLSFTLRLLAEL
                     GKKQDV"
     gene            45261..45572
                     /locus_tag="PK839_02735"
     CDS             45261..45572
                     /locus_tag="PK839_02735"
                     /inference="COORDINATES: protein motif:HMM:TIGR03342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HOG71683.1"
                     /translation="MSEKVYAGKSVTVTEDGYLTDPNQWTKDIAVEIAREEGITLSPK
                     HFEVIEFIRDKTLKGEVMTIRTIGKSGIIDTKGFYELFPGAPLKKASRIAGVSKPTSC
                     V"
     gene            45590..46093
                     /locus_tag="PK839_02740"
     CDS             45590..46093
                     /locus_tag="PK839_02740"
                     /inference="COORDINATES: protein motif:HMM:NF025066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HOG71684.1"
                     /translation="MAGTIDHPIKKVLIICAKANIEDVYAALVMANGAVMEGIETKMF
                     FTFFGLDAITKKSMKKLHTSAVGNPGLKMPGGLPFPTLLGILPGVEAGVSAMMKSQME
                     KLDIPTVEEFLELITAGGGEIYACKLAVDMFGLKKEDFSEHVKDIITVGQLYELAAGE
                     GSQIIFT"
     gene            46279..47406
                     /locus_tag="PK839_02745"
     CDS             46279..47406
                     /locus_tag="PK839_02745"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOG71685.1"
                     /translation="MEELVRLRHELHRNPELSGHESATAKRLRFFLSRYNPDELYSNI
                     AGEGMAVIYQGKEPGPTVLFRCDMDALPIAETNDVKYKSNVQGVSHVCGHDGHMTIIS
                     GLAVRFSQSKPKKGRVILFYQPSEENGEGARKSLEGLKELGLNPDYAIALHNLPGYKM
                     GSIILGRYTFSAASKGLVINLKGKPSHAAYPEHGLNPAAATAEIIQSLLSIQSTTHFK
                     NFILVTVIHIRVGDIAFGTSPGNATIMATLRAFDDDDMTLLSKIAAETATSIAHKHKL
                     GIDVSFTDEYPASVCDKTLTHTLEAIAHEASLEVNFLEQPNRWSEDFAHFSTCFPSVI
                     FGLGAGENHPDIHTPEYDFPDELIDNAVDFLGKIANKLMEG"
     gene            47740..48666
                     /locus_tag="PK839_02750"
     CDS             47740..48666
                     /locus_tag="PK839_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005862977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HOG71686.1"
                     /translation="MKSFWKRDLGLVPSEEWEPYCWVDVEIPTEEERKYLFDELGVPE
                     AFFNDIEDVDERPRIEFEDEWFFILLRMPYKNVDVSLPFTTIPVGIIFKDDIFVSISF
                     YRTEVIPDFIQFTIRKGIVIKDHFDLVLRLMLSSSVWFLKYLKQINLDIRLAEDQLEK
                     SIKNEDLQTLLSIEKCLVYFTTSLKGNDILLHRIKNLRNYKDTYNPDLIEDVEIELRQ
                     AQETTSVHSDILSGMMDAYASVISNNLNNVMRQLTSISIILMIPTLIASIYGMNVPNG
                     LENNPWGFGLVVLASFLFSILGIVLFRRKKWF"
     gene            complement(48777..49784)
                     /locus_tag="PK839_02755"
     CDS             complement(48777..49784)
                     /locus_tag="PK839_02755"
                     /inference="COORDINATES: protein motif:HMM:NF040411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6427 family protein"
                     /protein_id="HOG71687.1"
                     /translation="MLLKFFRRFRISTLLVMLGVITLFWAGTLMQKYPPSTFTFDSFG
                     MPLYVLIVKILSPYSFLSKLVAFALTLTVAIYLLQFNVRHTVVRQRTYLPFLFFVLVA
                     SSLTPLQRLNPAIIANIFILVSLSHVISTYQNSEALDHLFRSGLLLGIGTLLYAPTVV
                     FVFLIFWSIATLRKFNLREWLVTLVGILVPWGAVVFYFYWFDLGASEPFIQLANSLCL
                     EAESSADKIAPIILCVAVGVPLLVAILHMLTGMGSQKIAIRKHQNIMLLFTVVALLSF
                     FVVPSCSYEILYIAAAPISLLLSEYFSDAKGKYWPEILFLIFILGLVSMQLYPLLMHS
                     L"
     gene            complement(49801..51090)
                     /gene="purD"
                     /locus_tag="PK839_02760"
     CDS             complement(49801..51090)
                     /gene="purD"
                     /locus_tag="PK839_02760"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HOG71688.1"
                     /translation="MSGVNVLIIGSGGREHALAWKLSQSPKLGKLFALPGNPGTARLG
                     ENIPLSVLDFEGIRTVVLEKNINLVVIGPEEPLVKGLYNFLTGDLGLRNVLVVGPGRE
                     GAMLEGSKNFAKAFMQRHGIPTARYRAFTRDEYSEAKKFLGTLSSPYVLKADGLAAGK
                     GVVIQASYEDACASLGEFFSGKFGDAGNVVVIEEYLRGVELSVFVLTDGKSYVILPEA
                     KDYKRVGAGDTGPNTGGMGAVSPVPFATPEFMKKVEETIVKPTISGLASEGIPYCGFI
                     FFGLMNVVGNPFVVEYNARLGDPETQVVLPRIKSDMLELLMLCAAGKMNEAELVVAPQ
                     FAATVILASGGYPGSYEKGKAISGLEASANAIIFHAGTTSSGSMTLTAGGRVLACTGT
                     GESMEDALASSYCAANAITFESKYFRSDIGMDVYPKS"
     gene            51291..51776
                     /locus_tag="PK839_02765"
     CDS             51291..51776
                     /locus_tag="PK839_02765"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006952404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HOG71689.1"
                     /translation="MVISIVVATSENRVIGKNNQLLWHISEDLKRFKKLTTGHTVIMG
                     RKTFESIGQPLPNRHNIVISRSEGLKFKGCTAAKSLADALEIASTDEEVFIIGGGEVY
                     RQALPLAERIYLTLVHANYEGDTFFPALNPNEWKVVREEKVSPTEGPGISFLDYVRQH
                     S"
     gene            complement(51773..52999)
                     /gene="clpX"
                     /locus_tag="PK839_02770"
     CDS             complement(51773..52999)
                     /gene="clpX"
                     /locus_tag="PK839_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004294102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HOG71690.1"
                     /translation="MVKNTDICSFCGRSRKEVSLLIAGMDGFICDLCVEQASIIVSEE
                     LKKKGEFKLQKSKLMRPTEIKKFLDQYVIGQDEAKKYLSVAVYNHYKRLLQKDMGDDV
                     EIEKSNILLVGETGTGKTLLARTIARMLHVPFTIVDATVLTEAGYVGEDVESILTRLL
                     QVADYNVEAAEKGIVFIDEIDKIARKGDNPSITRDVSGEGVQQALLKLLEGSVVNVPP
                     QGGRKHPEQKMIAVDTKNILFICGGAFEGIEKKIAQRLNTTVVGYGAGKTREVIDKNN
                     LLQYIAPQDLRAYGLIPEIIGRLPVLTFLSPLDKVALRKILTEPKNAIIKQYIKLFEY
                     DGIKLQFEEEVLDYIVNKAVEFKLGARGLRSICEAIMMDAMFDLPTGSEKEITVNLDY
                     AQQKLEKSNLKRLKAA"
     gene            complement(53182..53862)
                     /locus_tag="PK839_02775"
     CDS             complement(53182..53862)
                     /locus_tag="PK839_02775"
                     /inference="COORDINATES: protein motif:HMM:NF013357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH25 family lysozyme"
                     /protein_id="HOG71691.1"
                     /translation="MPVAVFLSAALMLGIIRFISKPDHRYVIRGIDVSAHTGSISWEK
                     LKDQRVYFAFVKASEGATLKDKNFQDNVNGARAAGIPVGAYHFFNFNRDGLTQANNFL
                     STIGRQKYNLPLVVDVEEWGNNIRRSRSQIIADLREFVSKVEKNRYQKIIIYTNEDCY
                     KLYIQDVFPDNDIWICSFNHKPKLNREWTFWQYEHNGRLKGVKGKVDFNVFRGSMLEW
                     RQYIEDGR"
     gene            complement(53873..54220)
                     /locus_tag="PK839_02780"
     CDS             complement(53873..54220)
                     /locus_tag="PK839_02780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG71692.1"
                     /translation="MAVILVNRIGWIRIFTKIKKHSKLHEKVDKVESCRVVMGNGLKM
                     THPKLHEISIYSCTGDLCFWLQNSAVRPIPNIGFSSIVNGIKCKDFPTIVARNFERLY
                     LCGISYGKKIEEA"
     gene            54195..56978
                     /locus_tag="PK839_02785"
     CDS             54195..56978
                     /locus_tag="PK839_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG71693.1"
                     /translation="MRLTRITAILFFSCIALAANGQEIGMTGTKANAERVMADSLLKN
                     QQYAQALPIYSRLLEQHSRDPQIQLNLGICYLYGTRRMDLAVKFLSQASTGDVSSIVY
                     FYLAEALRQSYRFSEAIDYYRRFTISGGASDIKLEEVEQLVTLCENGSFLTRYIYNPE
                     VYDTKAVAAGEACTFYSIAPKSGSFVNAPTNLLTPTDIKENHQPLMFYPTNPTPGTYV
                     YYSSYGSTTTYGKDIFRIQLQDNGSWSKPENLGDVVNSNFDEDYPYIIPDGVTLYFAS
                     KGHYGMGGYDIFRTVFDPTTKRWSPPENLAFPINSPFDDFLFVADDSDSLACFATNRK
                     VMPDTVEVILMRNEQNPIRRSPRSYSELDSIAALKVSTLALNAPTKNPTTVQAEKPIA
                     ASQKPASFNSVENDPEYSRTIAKGFAAQQLADSLKIRLEGLRGKFDFVTTAEQRIRLE
                     KQVVQVEDDMLAAQKEADLMFVRASQIEQEYLTGKRKPKGDESLSFVSDNPDYIYQAQ
                     FAPTVFQTDELNRLAQAEQLYPQVLRNRNLALTNRDIYDQCKKNSTDSSTKVCDTKYA
                     DMLSSMQSYNEQMGKYYEKKYPIYNDCVHVALVKSGSNDQSIRKCISTAYSHFRAAGT
                     ILNNMGNDGRVESAFEANLLRELGLLQLDLAFAKIWHLQLFEQKLTSSIIKFERSAFG
                     STSAIELLSAEQSKPQQAAASSYTPPRIERVSSPVNIENLAVESEISSDFGIAKTSPY
                     NEENPIPAVSALPNGICYKIQLGVYGTPRQPQFFKGIYPISAEPVGKLTRYYAGVFAR
                     LADAESALSKVKSVGFRDAFIVAWYNGQQVSLQRAQQLEGSLQQQKSSDSDRKGVFAI
                     QLGVYEGSMPNDELKTIRTLIPNKDITRRVDERGLMVYSIGNFSTLSEAERNKDNLIA
                     GGILNAKVIELNQ"
     gene            57117..57398
                     /locus_tag="PK839_02790"
     CDS             57117..57398
                     /locus_tag="PK839_02790"
                     /inference="COORDINATES: protein motif:HMM:NF014655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HOG71694.1"
                     /translation="MKVKEKTFGLEHTQNKSSEVYTLFLHNDDVNTFEFVIQALVEVC
                     RQDENQAEQCALITHFKGKCDIKRGSLPELEPMMEELHRRGLSASINLN"
     gene            57415..57876
                     /locus_tag="PK839_02795"
     CDS             57415..57876
                     /locus_tag="PK839_02795"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HOG71695.1"
                     /translation="MIWIFLLILLGLFLLVAEVLLVPGITVAGIAGFLLIGYGVFAAY
                     TEHGTIAGHITLVFSIVASVITIVLSLRSKTWKKITLNTSIDSKANMDYEQHIQPGDT
                     GVTIGRLAPMGKATINGHDVEVTALDELVDPQTTVVVVKVEGSKIIVKPKK"
     gene            57882..58862
                     /gene="floA"
                     /locus_tag="PK839_02800"
     CDS             57882..58862
                     /gene="floA"
                     /locus_tag="PK839_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137107.1"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HOG71696.1"
                     /translation="MSQVGAILTIIAVCIVGLWIIFYFVPVGLWFQALVSGVRISLLQ
                     LIFMRWRKVPPRVIVQAMIEGTKAGLTLNRNEMEAHYLAGGRVPKVVHALVSAQKANI
                     PLDFKMATAIDLAGRDVFEAVQMSVNPKVINTPPVTAVAKDGIQLITKARVTVRANIK
                     QLVGGAGEETVLARVGEGIVTAIGSSQNHKEVLENPDKISKTVLNKGLDAGTAFEILS
                     IDIADIDIGRNIGAVLQMDQANADKNIAQAKAEERRAMAVALEQEMKAKAQEARAKVI
                     EAEAQIPLAMADAFKSGNLGIMDYYRIKNIEADTNMRDSIAKPGVEPKKK"
     assembly_gap    58960..59059
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            59374..60339
                     /locus_tag="PK839_02805"
     CDS             59374..60339
                     /locus_tag="PK839_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOG71697.1"
                     /translation="MKNILVVGAGGQIGSELVPYLRKIYGNEHVVAADINSSACTHLA
                     EEGPFEELDALKADRYLEIVKKYRIDTIFNLVALLSATGEKKPQLAWHVNMGALMNSL
                     EIARENSCAVFTPSSIGAFGPTSPLDNTPQDTVMRPTTIYGICKVTGELLSDYYFLKY
                     GVDTRSVRFPGIISNVTLPGGGTTDYAVEIFYGAVQHKHYVCPLPEGTYLDMMYMPDA
                     LDACVQLMEADPSKLKHRNSFNVTAMSFEPSQVYSAIRRRIPDFTMEYSVDPVKKAIA
                     DSWPNYMDDSCARQEWGWSPKWNLETMTDDMLRAIGEKMKKGLLG"
BASE COUNT        17595 a        14273 c        13376 g        15205 t
ORIGIN      
        1 cgtcgttagc caggcaattt tttatccttt atcctttaac ctttatcctt gtattaccct
       61 ttatccttgt cttgcctttt cccagcagag cgtaaaagtt caaaaaaaag ccttacttgt
      121 tgatatttat tgccagcagc agtggctagc atatttggaa taatacctac ttttgctgca
      181 aattcaaacg tagctatgag cgtaggttac tcggaggaga gcattaaaac acttgaatgg
      241 caggatcata ttcgcaagcg gccggggatg tacatcggca agctgggcaa cggctctcag
      301 cccgatgatg gtatttacgt tctacttaaa gaggtagttg acaactccgt tgatgagttc
      361 atgatgggcg ccggtaaacg aattgaaata tccgtagttg gtcataccgt ttctgttcga
      421 gactacggaa ggggcatccc gcagggaaaa ctcatggatg tggcctctaa aatgaatacc
      481 ggagctaagt acgactctaa ggcatttaaa aaatcggttg ggcttaacgg tgtaggaata
      541 aaggctgtaa atgccctatc gaaaaagttc atcatacgta gcattcgcga gggcattgcc
      601 aaggaaatag agttcagcgc agggctcccc gttagagagc aaaactacgc ctccaatgga
      661 gagcttaccg gaacattcat cgagttttta cccgatgatg ccgttttcgg cgaatacgcc
      721 tttcaggatg agtacattga gcagatgatc cgtaactaca cctacctgaa caccaacctt
      781 accatcacct tcaacggcaa ggagtttgta tcgaaaaacg ggctgctcga cctgcttaac
      841 gaaaaccttg gcggcgaacc gctctacccc attatccacc ttaccggtaa cgatattgag
      901 gtggccatta cgcacagtag tggttatggt gaagactact acacctttgt taacggacag
      961 cacaccattc agggaggcac ccacctaacc gcattcaggg aatcctacgt taaaaccatt
     1021 cgggaattct acaaaaagga cttcgatccg gccgatgttc gaatgtcgct cattgcggcc
     1081 atcagcataa aggtggaaga gcctattttt gaatcgcaaa ccaaaacaaa gctgggaagc
     1141 aaggatatga gccccgacgg gccatccatt aggcagttca ttggtgattt tattaaaaag
     1201 gagctggaca actaccttca caagcacagc gaaaccgcca gcatcctgct ccaaaagatt
     1261 cttgagtcgg aaaaggagcg taaggctatc tccggaataa aaaaaattgc ccgtgaacgg
     1321 gcaaaaaagg ccagcctgca taacaagaag ctgcgcgatt gccgtgttca ctacaacacc
     1381 agcgatcccc gtgccgagga gtcaaccctg tttatcaccg agggggattc ggccagcggt
     1441 tccattacca agtcgcgcga tgtgaacacc caagccgttt tctccctgaa ggggaaaccc
     1501 ctcaacacct acggcctgac caagaaaata gtttacgaga acgaagaatt caacctgctg
     1561 caatcggcgc tggacattga ggacgacatg gacaacctcc gctacaaccg cattgtgata
     1621 gccaccgatg ccgatgttga cgggatgcac atccggctac tgctcatcag ctttttcctg
     1681 caattcttcc ccgagctaat ccggcaggag caccttttca tccttcaaac gccgctgttc
     1741 agggtaagga ataagaaaaa aacatcatac tgctactctt ccgaagagcg cgagaaggcc
     1801 attagggaac ttggccctaa ccccgaaatt acccgattta aaggccttgg tgaaatatca
     1861 cccgaagaat ttgccaactt cattggcccc gacatgcggc tcgaccccgt tcggcttacc
     1921 aaggacgacc aaatatttgc cctgctcgaa ttctacatgg gtaaaaatac ccctttacgt
     1981 caggatttca tcatcaataa cctcagaata gaagaggata tagtggagga ggatactatt
     2041 cattttgaag atgggaatgt tacggctgag gcaagctttc agcacgatac cctggtataa
     2101 ctaacaaacg ctgcagcacg ccatgcgact tgacgatttt gatgccgacg agctactcgt
     2161 tggcggcgaa aaaaatactt cacccgaaga gcaagccaaa attgaaaggc taaccgccga
     2221 tacggtaaaa agaacccagc taaccggaat gtacaaaaac tggtttcttg actacgcctc
     2281 gtacgttatt ctggaacggg ctgtacctca ccttgacgac gggctgaagc ctgttcagcg
     2341 tcgcattcta catgccatga aacgccttga tgatggccgc tacaacaagg tggccaatat
     2401 aattggctac accatgcagt accatcccca tggcgacgcc tccattggtg atgcgcttgt
     2461 tcaactgggg caaaaaaacc tgctggttga cgttcagggt aactggggca acatccttac
     2521 gggtgatgga gcggctgctc cccggtacat agaggcacgc ctgtcgaaat ttgccaacga
     2581 cgtggtgttt aaccctaaaa ccaccgagtg gatgctcagc tacgacggta gaaaccagga
     2641 gcccgttact ctccccgtaa agtttccact gttgctggca caaggcgttg agggcattgc
     2701 cgtcggattg gcctcaaaaa ttctgcccca caacttcaac gagctgattg atgcagccgt
     2761 tgactacctc agaggtaaag aatttgagat ctaccccgat ttcccaacag gcggcatggc
     2821 cgattgcagc cgttacaacg atggcctcag gggcggctcg gttaagttgc gtgcccgtat
     2881 tcaaaaagtt gaccggaaaa cattggtcat caccgagtta ccctttggca aaactacctc
     2941 atcgctcatc gaatctattg ttaaggccag cgaaaaggga aaaatcaaaa taaagagaat
     3001 tgacgacaac acggcgggaa atgttgaaat actcattcac cttccgccgg aggccaatcc
     3061 cgatatgacc atcgacgcca tgtacgcctt taccgactgt gaaatatcca tttctaccaa
     3121 cgcatgcgtt atttacgagg acaaaccatg cttcatgggg gttaaggaca ttctaaaagc
     3181 ttcggttgag cggaccaagg agctgcttac ccgcgagctg aacatccggt tgggcgaact
     3241 tgaagaggag tggcatttct cctcgcttga gaaggtattc tttgaaaagc ggatataccg
     3301 tgagctggag aaggataccg aaacatggga tatggtgatt gacgccattg agcgaggttt
     3361 tgacccctac aggaaacttt tccgccgacc aataacccgc gacgatgtgc tgaagcttac
     3421 ggaaaaacct gtcaggaaaa tttcaaaatt cgacataaag aaagccgacg agcacattct
     3481 tgccgtggag gctgaaatgg cagaggttgg aaaaaacctg gccaacgttg tggattacac
     3541 cattaaccac taccagcaaa taaagaagaa gtacggtaaa ggcagggaac gcaaaaccga
     3601 gcttcgcagc ttcgacagca ttgaggccac cagcgttgtt gctgccaacg agaaactata
     3661 cgttaaccgt tccgagggct ttgtgggaac cggccttaaa aaagacgagt acatctgcga
     3721 ttgctccgac attgatgaca ttatcgtttt cctgcgcaac ggaaagtatg ttgttcagaa
     3781 ggtgcaggaa aaggcatttg ttggcaagga catcatccat gtggcagttt acaggaaaaa
     3841 tgacaggcaa actacctaca acgtgctgta ccgtgatggt ttaaatggtg atatcatgat
     3901 gaagcgctgc accataagcg gcaccacccg cgataaggag tacgacatca ccaagggaac
     3961 agctggctca cagattctct actttaacgc caactccaac ggcgaaacag atattttaaa
     4021 ggttttcctc aaacccaaac ctcgccttcg aaatctgatt cttgagctgg attttagcca
     4081 gctggctgtg aagggaaggg actcgcaggg caacattttt acccgccatg ccattcataa
     4141 aattcaggtg aaggaaaaat tagccggcca ggtagaagcc caaaaagtat ggtacgaccc
     4201 cgaaacactc cggctctaca aggaggcaaa aggtaatttt ttaggtgagt ttatgcccgg
     4261 cgaaaaaatt ttgatagcaa atagcgatgg aacctatttc gtggccggaa cggagtactc
     4321 cctgcgattt aacgctcctc cccttgtggt ggaaaaattc aaccccaaca agatttactc
     4381 ggctgtttac ttcgatggtg agcaaaacta cttttacctt aagcggttca cctttgaacc
     4441 caccgagaaa atccaaccct ttattcccga cgatggcaag tcgtaccttg tggagctgaa
     4501 cagcgattta taccccaacc tactggtacg attcggtgga aagcatgcca gtcaccaacc
     4561 cgaaaccatt gatgtggatg catttatagc agctaaaagt taccgcgcta aggggaaacg
     4621 aatttccacc ctcaacgtta aaaaagtaga gtttaccgaa cccctgcaaa aggaagcttc
     4681 agcctttagc actatagccg gcaataacaa tgcacaactc gaagaagaag atgacggagg
     4741 agctgctgaa gcagttggta agtagcaaag tagcgctaca tgctagctca catggcaaag
     4801 ttcttaccct ggcatctaat tagccctgca gccagctaca ccaagctgca acttttcaat
     4861 ttattttgaa atttttaata atgatgtacc tttgccaatc gatatgtaat aattttcata
     4921 acaaaaagac acctagcctc ttaacaagga aggagaacat aactcatcaa cgttgaaatg
     4981 aagagggggg ccgtgatagc tgtagaaata cgattttaaa ctcgaggaaa agtgcatttg
     5041 atacgcatat taaaccaaat gttaaactac tcaaaaacag cattaaaagt atgaaaacaa
     5101 atcttgttaa ggttgccctt atggcagctg catttgtcgt tggtttttcg agttgtgacg
     5161 acgacgatcc aacctacacc gtaacccttg ccgtaaatcc atccaacgca ggaaccgtta
     5221 ccggtggagg ggattacaaa aagggcgaca ttatccagct tactgccaca gccaacgaga
     5281 atttctactt tgtaagctgg acagaaggaa ccaacgagct aagcaccgaa gccaccttcg
     5341 attacactgt tggggataag gatgttacgc taaccgccaa tttcaacgag aaaaaaattg
     5401 ttacactagg tgcacaatcc aacacaactt ttccaggatt tctttccgtt tccgaaggtg
     5461 ccacctataa ctttaacgaa gcttccagcc accaggcagc tgttgatatt ttttgcttct
     5521 atgaggtggg gaatgatata gcgctggcca gccctggctc aaacatcacc ggaatctttg
     5581 ggggaaccga aaccgaccct gccaactgga caaccaaaaa caccaccctt tttaaccagg
     5641 taaccactct tactgtcgcc cagtttgaca ccatccccaa cggcgatgca aatattcaaa
     5701 cttactatag tgccgatgca gctcgcaaaa aggcaaaatt gcttataaag gacaacatct
     5761 acgcctttaa aactcagaat ggaacctacg gactgctaaa ggttatagat gtaagcgtag
     5821 gagccgatgg ttttataaaa tttgaataca aaacaaaata accagttaat tttaaactca
     5881 ttaaccctga aaggaagcaa acttttcggg gttactattt tttttaaaac tatgcgaaag
     5941 ctaactattt gcctaatagc aattttcctg ctagcagctt gcagtgaaaa agagtcggtg
     6001 gatacccctc catcaattac ttttcaaacc gggagctaca cgcaggatgg tgaagcagtt
     6061 cctctaggcg gtaagctaac gttcggaatc actgcaaatg gaggaagtga gccacttaca
     6121 aatatcagaa ttcaacgggt agccggaggc aacgttatta ctgaggtaga taaggggtta
     6181 ttcgtaaaaa acgaagacta cccttacgtg gttaatgcag ttaaatctga tgcttcggag
     6241 gaggtgtggc gatttatggc catgaattcc aagcgcgaca gtgttgaaat tacccgaacc
     6301 gtgcttttag gtgaaggtgt agcatacgga ccaataaggc atttcccctc cgtgaaaatt
     6361 ggaatgcaga acaacacgga gtacccacac tttctctgcc taagcactgg caccacctac
     6421 agcaatgaag aggttgccgg caacgaggct tccattgatt tgctggcatt tgtttaccaa
     6481 accagcggca tctggtcgcc aacccttaac tgcccgcagt acacaaccgc accaagctac
     6541 taccccattg tgggcagctg gacaacacga aactcaacac tctacgacta caacgcagtg
     6601 gataacaacc tggttagcct tgatgaattt aatgccgcta ccaacgacag cctgctggtg
     6661 gcttcattta aacctcaaag ttcaagcgga aattgcaagt tctgcttcac cggtaaaatt
     6721 gtccctttca aaacccagct gggcaagtac ggtcttatta ggattataca tgccgatcag
     6781 gttgcagaag gatacatgga gctggaaatt aaaattcagg agtagttgag aatggttgct
     6841 gctaattaaa cggcatcacc tgtataaaaa agggaaggta aaattatttc cataggaaag
     6901 catagaaata gaagtaaaaa tcaacaaagg tgaaattata acacaggaaa aattagtgat
     6961 gaaacacgat agcactacta ctgtttggga taaatacagc tgttcaaagg gctggcaatt
     7021 tcaactaact aaggccgcta aaatagtagc tttagcttcg gcattgatgc tgttcttccc
     7081 catcatcacc catgcccaaa actccatcac gggaaccatt aaggataagg caaccggcga
     7141 tccccttgca ggggttcatg tgtcggttgg acaacaggtt gccaccacta actcgcaagg
     7201 tttttatgta ttgaaaaacc taaaacccgg aacccacata gttactgcct cactggtagg
     7261 gtataagtca gaacaacaac ctgccaagat tgtaaacgaa tcaatcaccc tcaacttttt
     7321 tcttgaggaa gatttgatat ttctcaacga ggtaaccgtt tcggctaccc gaaccgaaag
     7381 ccgaatagca gatattcctg gccgtattga gatgataacc cccgagaaaa tatcgcttgc
     7441 ctcctaccag agcgttgacg agttgctgac gcttattcct ggggtgcata cagcacgctc
     7501 attcggactt ttctcctacc gctcatccgt ttcgatgagg ggcgtaagct ctaaggagca
     7561 gggacgaacc cttgtgctgc tcgatggaat cccaatgaat aaggccgatg gtggctctgt
     7621 gaactggaac ctgatatcaa caagagaaat cgagcgcata gaggtggtta aagggccagg
     7681 ttccgccctt tacggtggaa atgccatggg tggaattatt aatgttgtta cccaaaaacc
     7741 tacccgtcca ttggagggta gcgttgcggg cagctatggc acctacaaca ccaaaggggt
     7801 gaacgctaaa ctggcaggca agctgaacaa cggattctac tgggcggccg atgggttatt
     7861 ccgcaacagc gacggctaca ttacccagtc aatagccgac aggctcacaa acccatacat
     7921 tgtgaaatcc acctttgagg aaaaggtgct gcatctgaga accgggtatg ataatgggca
     7981 gaagctgcag gccgatgtag attttacgct gtttgacgat gttcgcacaa ctggcgaaaa
     8041 ggtttaccag ccgcagggca acacacgtga gtacgacacc taccagcttc gggcaaacgt
     8101 aaagggagtt gccggagccg ttgtgtggaa cgtttcggca ttctttctga atgaggacta
     8161 caagcgcgta agcgaatgga tgaaagatga ctacacttgg tacgatgtgc tttccgttcg
     8221 tcgcgattat ggattgctca ccagcgcaaa ctacagagca ggaaggcata cgtttaccgg
     8281 tggagttgac ctgagaatgg gcagcgttga tgcctccgat gtttactaca cctcaaccga
     8341 tcgggttgac aacaacggca agatgaactt ttacggtttc tacctgcaag atgtagtggc
     8401 tcttttcgac agcaccgtga atgtaactgc cggtttgagg tacgactacg cacgattctt
     8461 cgacggccaa ttccagattc tttcaccttc agccgaaaca gctttcatga gccagtacca
     8521 gttttcccag aacggttcag aaagctgggg ggcattaagt ccacgaattt ctgcccaatt
     8581 caaacctaac ggcaactaca gaatatacgc cagctacagc aggggtttca gaccttccgt
     8641 ccttgacgat ctatgccgct cgggacgcgt taagggtggt tttaaggtag caaaccccaa
     8701 ccttaaaccc gaatacctcg acaacttcga agtggggggc gattataaac catttgatcg
     8761 cttacgctta gccgcaagcg cctactattc cactggcaaa gatttcctat actacgtttc
     8821 caccggcgac agcattgaca tggggtttgg acctcgacca ataatgatac cctccaacat
     8881 ttcggatgtg gaaatatacg gcatggaggc agaattttcg gctactccct ggaggttcct
     8941 caccctgtcg ggaggctatg cctacgcctt atccaaaatt acaggatata agcctatcgc
     9001 agccaacgac tttgacctta ccggaaagta tcttaccgat gtgccaaaac actcgttcaa
     9061 cgccggagca ttcctgcgtt cgcatattgt aaatgcagga gttaccgcca actacaccgg
     9121 cagaatgtac atcaacgacc aaaacgctta tgatgatata gttctgagca accagcttcc
     9181 ggcagccttt accctcgacc tgaagatctc ccgtgaattt ttcggccatg tgaacatctc
     9241 acttaacatt cagaatattc tggataaaaa aatttacgaa agtagcatgt cggttggccc
     9301 gggacgtttc atgatgctgg aggtaaaggc aaacctatag ttttatacct atttgggata
     9361 accataattg tattttatct aacctgataa actgatgaag agaattatac ttgctgcaag
     9421 tgcgttgttc atggcatcac tactgccatc gtgcggaaac catcccgatg gcatatcggg
     9481 agcaaccaag gattacgact cgaacggaaa cagcttctac caccggacaa ccgaaaccaa
     9541 gctaaaaacc tgcgacttac gggtagaggg cgaggttcgt aaacccgggg aaatcaacct
     9601 taaacactac ttcaagcgag aggtggttta caagcaggca gtgctgaacg attcaggaca
     9661 agtagcgttt accggcgcat accgctaccg gggctactcc cttttcgatc tgcttaaccc
     9721 gtttgtcctg gagaaaaaaa acgccaagga atttgttccg gcaaccgatg tttacatcat
     9781 tattgaaaac aattcgggcg acagggtggt attctcatgg gctgaaattt tccttggtca
     9841 caacatgcac caggtgctaa ttgccaccga acaggccgac gttgaaccct acaaggttaa
     9901 agtcagctac ccgaaggaca gtgtttggaa ggtagtggca gccaacgact tgtttgctta
     9961 ccgcgagttg aaaaatcctt caagaataat tgtgaagtca ttcgacagga agtactatga
    10021 aatcaacagg gagctgaaag atccatttag cccaacggtt aacttggtgg ttgacgactc
    10081 cctgatggga gttatagaca ccctgaacgc agttgcacct catgcccgct accattcagt
    10141 gttttacggt atgggcatgg gctaccacgg tacgccaacc tttgagggac cgctgcttcg
    10201 acccttggtt gaaaatttcc tcgccaaaga cggggcaaaa tggatgcgga caggcatagc
    10261 ctgcgtggtg ggaaaagatg gctttagaaa catatttagc gtaagcgagc tgttcaaccg
    10321 ggttgatcag gttgaaccga ttctggccat acccgaaaat ccgaagaaaa gtggttacta
    10381 ccgtctttac cacccgtcag ctttttacgc cgatttctcg gtaagaaacc tgtcggagat
    10441 ttacctgttc cgggagtgag agggttgctg tgttgcccct cctgtttgga atgccgtttt
    10501 atcgggaaaa gcatcattca aacagctaga aaccattttg aatggcctcc attatcttgt
    10561 tcagcttatc ttccaaaggg agttcgccat taatccaggt aatgcttagc ccacggcgtt
    10621 ccatgccacg aaaccaggtc atttgccgct tggcaaattg cctgatagcg atgttcaagc
    10681 ggctaaccat ttcgtcgtag gttaacttgc ccatgatgtg ctcggcgatg tagcggtact
    10741 ccaagccgta gtaagccata tcgtcaaaac ttagccccga ctccatcaac cccctcacct
    10801 catctaccat accgttcccg agccgttgta gcagccgctg atggattcgc tccatttcgg
    10861 tagcccggtt ataccttacg ccgaatatgg cacttttaag ttgagggaat ggttcaattt
    10921 cggtaggagc gttcaggtag tactcctcaa tttcgatagc cctgatagtt cgcttacggg
    10981 tgtcgagatc ggtggtgttg tgaagagttt tgtaggatgc aagaatttta gccagctcgt
    11041 cgttcgactt agcctcaagc tccccacgga gggttgcgtt ctggggaact ggcaaaagcc
    11101 ggtaacccct taacacagcc tctatgtata gcccgcttcc tccacaaagt ataggcaaaa
    11161 ccttgcgcgc tttcaggcta cggaatacct tcagaaaatc ggactggtac tcataaacat
    11221 tgtagtgata gccggcatca accaagtcaa ccagatggta gggtatcgtt ttgccattta
    11281 ccacgtagtc ctcatagtct ttaccggttc caaggttcat gccgcggtaa acctgtcggg
    11341 agtcggcgct aataatttct ccatttaccc gcgatgccag attagctgca agcagggttt
    11401 taccactagc agttgggcct acaatcgcaa taatatcgta cgggcaattg tccatttatc
    11461 ttttgggtga taaagcgtaa atttagctat ttttgcgccc tacaaacatt ctaacagcca
    11521 agtcaccatc ctatcccaat ttgtaatttt atccaccttt aaataaccag caccatggca
    11581 gcgctacgaa ggaataaccg gcaaagcagg catgaagagc taaacaggct aatctggatg
    11641 ggaattgtaa ttgccgttac attcatattt gttattgctg ttgcaacaga catgttcagg
    11701 ataattccgc ttaaacgaac caacattgta atgctggtgt tggcattgct aattgtggcg
    11761 gtttatgttt acagctggat aaagttttac agaaaaagga agcagtagcc acatactccc
    11821 atgagtaacg aaattgttgt taaaaacaag aaggcaacct ttcagttcga gattgtcgaa
    11881 aagttcaccg ccggaattgt gcttaaagga acggaaatta aatccatccg aatgggcagg
    11941 gccagctttg tggattcgta ctgctttttc attggtgatg aactatgggt taaggggctt
    12001 catatagcgg agtatgcctg gggtacctac aacaaccacg accccaaaca ggatagaaag
    12061 ctgctgttaa ccagaaagga gctcactaag ctgaaacggc ggtcacagga gaaaggactc
    12121 accattatag ccctgaaact ttttatcaac gaacggggtt tggccaaggt tgatattgct
    12181 ttggcacggg gaaagcagct gcacgacaag cgggacgacc tgagggaaaa ggacgttcgt
    12241 cgtgagctcg atcgtgcccg taaaaggtag ccatcatctt ttttcttacc ctgtttcttc
    12301 taaaggattt tcattctcaa gctaattacc agcgcataaa attcaaattt tgaaaaaatt
    12361 ccattaggtt tgtaaagtgc aataaaaaag gataaccagc aaagcaaatg ggtaaggtaa
    12421 agttaaccgt tttaggtatt tcatacagtc aaactcagtc tggtgcctac gccttagtct
    12481 tgagcgaaga agacgggaac aggagaattc cgataataat tggtggctac gaggcgcaag
    12541 ccattgcaat tcaactcgag gggttaaccc caccacgccc gttgacccac gacttattca
    12601 tgaattttgc taaagcttac catatagaaa ttctggatgt caacatctac aagctggagg
    12661 agggtgtgtt tttttcaaaa ctacactgca ccaatggcga taaggagata tttgttgatt
    12721 ccagaacctc cgatgctgta gcgttggcac taaggtttga ttgccccatc tacaccaccg
    12781 aggaaataat tgaaaaagcc gggcttaccc tcgaaattga gaatattgac gaggatgccg
    12841 ctaaggagga tgagggagaa aagggaaatg cccccgttga cagcttcatg gagcaactcg
    12901 ccaagctatc tgtttcggaa ctaaagacta tgctcgacga agccgttcgg aacgaggact
    12961 acgaaaaggc ttcaaaaatt cgcgacgagc taaagaaccg ctcctgaaaa aaataaagtc
    13021 aaacattacc tatgggcatc aagcagaagc cccatcctga caggatgggg cttctggcaa
    13081 aatacttctc tattgtgcag ccttacttac ggtggacgaa ttctttgggt tgtgcttgta
    13141 cttaaaaaca aacgcaaaca caattgccac taccaacgag taggccgaaa atacaaacca
    13201 ggcatcaggc cagcctgttg catcaacaac cagccctgca atatatgagc ctataaatgc
    13261 tccaaaacca ttggtcataa tcataaaaac cccctgcgcg ctggaacgga ttttcgcatc
    13321 ggtctcattt tccacaaaaa gtgaccccga aatgttgaaa aagtcgaatg caacgccata
    13381 cacaatcatg gaaagaatca gcatccaaac tcctgaacct gggttcccca accccagcag
    13441 accaaaacga agtacccatg caaacattga tatcagcata acctttttaa taccaaaccg
    13501 gctaaggaaa aacggtatca gcagaatgca aagggtttct gaaatctgcg atagcgagat
    13561 gagtatgttg gcatgtttaa caccaaatgt tccctggtac tcgctaattt tgccaaagta
    13621 gttggtgagg tagtcgttgg cgtaggcgtt ggttatctgc aacgacatgc ctagcagcat
    13681 ggcaaaaata aagaatacgg ccatcttgcg ctccttaaac aaggtaaagg cacgcagccc
    13741 taaagagtca accagcgatt ttttctctac atttttattc acctcacagt tggggagcgt
    13801 aaatgagtaa attcctaaaa ttaaccccag ccccgccgaa aggtaaaact ggtaggaggt
    13861 tgtggcaaag cccagcaggt caaccaaaat catagcgcaa ataaaaccca ccgttcccca
    13921 tacccgaatg ggaggaaatg cctttaccgt atcgtaccca ttgagcgata gggcattata
    13981 ggcaaccgag tttgataggg ctatagtagg catgtagaac ataacgctta gcagtaccag
    14041 tggatacaag gtgccgtact gcgtttgggg agcagcagca atcagaaagg cggctgcaag
    14101 aatatggcaa atgcccagca ccttttgggc aggaatccac ctatcggcaa tgatgcccat
    14161 tattgcaggc atgaaaagcg aagcaatacc catcgttgca aaaaaactcc caatctggaa
    14221 acccgaaaaa tgtaagcctc ctcccaaata tgcaccgagg gaaattaacc acgaacccca
    14281 aatggcatac tgcagaaagc tcatcagcgt taaacgcaac tttaacttca taacttttta
    14341 tatttagcgt tatcgatttt tttaaagtcc taaaaatagc attgttttat ttataaggtt
    14401 ttcgataagg gtagaaatgt tttctgttaa aagggcatat cacagcataa actaaacata
    14461 tccaataaat attttatgaa atttaccgca tttttccacc cttttgcata acaatgatgg
    14521 ccatatttaa ttttaaccca caagcacatg ctaaagcacc actagctcaa gcccaatttc
    14581 taaaaatatc gtcggaatga atatggcaac acctcagcag atactcacca aagcaaacag
    14641 gctaaagctt aaatacccgg tttgcttttc cattgctacc atgtaggctg gtagctgcag
    14701 ccaatccgca ttgaacctgc aatatgtttt ttttcttcta catcctaaat gtggaagatt
    14761 ttccactaaa tttcgggcaa agcgctgtag tgttgtcaat aaaatataat tttatcccaa
    14821 atttattgct ggctttgatt gctagcctac aataagaaag ttcaacatta acatgcggca
    14881 aatcaggcta cttttacttc ccattgcatg gctttactgg tgcggagtaa gaattcgcaa
    14941 catcctgttc gatgcaggta ttctcaaggg tttcagtccc gacattccaa ccatttgcgt
    15001 gggtaacctg gctgtagggg gaaccggcaa aacacccatg acggcctacc tttcatccct
    15061 actgcatgga aattttaatg ttgcagtgct aagccggggg tataaaagaa agaccaaggg
    15121 tttcaatata gtaagtaccg aatcaaccgt tcacgaagta ggcgacgagc ctcttttgct
    15181 aaaactccgt caaccgtcgg ctatcgtagc cgttgacgag gaccgtatga atggcatggt
    15241 ggagttgttt tacgcctatc ccgagctgga tgcaattatc cttgacgatg cctttcagca
    15301 ccgttggata aaaccaggat taaacctgct tttgaccgaa tatgagaacc tttacaccca
    15361 cgattttccc cttccggcag gcaggctacg cgacagccta tcggagcgaa aacgtgcaaa
    15421 tctcattatt gttaccaaat gccccgaaaa cctcaccgcc gataggtgca gggagatttc
    15481 cgcagaactt aagccaaacc caactcaaat catttacttc accagcatgg agtacggtat
    15541 gccccaacca gtatttagcg aaagcgcagg gaaacttaag ctaaccgaca tgcaaccggt
    15601 ttttgccatg acgggaattg ccaaccccaa acctttcgtt gattacctgc aaaagcatac
    15661 agcattgctt gccgccaaag catttgccga tcaccattac tattccgaag atgagatcga
    15721 ggctattttt acggagcttg ccaggtcaac cagccaggct gtagtggtta caactgaaaa
    15781 agacgcagtt cgcctgctga agttgaagtt gccggaggaa attaaaaaac ggatatacta
    15841 tattccggtg agcgtgaagt ttctattcaa cagttcagaa gaatttaata accaaatact
    15901 tagttatgtt agagaaaata aatcagacag tagctttcat taaggggaag gttaccattg
    15961 aacccgaagt tggaattatt cttggaaccg ggcttggcgg cctggtaaag gagatagaaa
    16021 agcaacaggc attggattac aaggatattc caaactttcc gatatcaacg gttgaagggc
    16081 acaccggacg cctgattttc ggtgagctag gtggtaagaa agtggtggcc atgcagggac
    16141 gcttccactt ctacgaaggt tatgatatga agcaggttac cttccctgtg agggtgatga
    16201 agtttctggg catccagtac ctttttgtat cgaatgccag cggtggtatg aatcccgcct
    16261 ttgaaattgg tgacctgatg atcatcaacg accacatcaa cctgatgcct aatccccttc
    16321 ttgggcctaa tataagtgag ctaggtccaa gattcccgga tatgcatagg gcttacgatg
    16381 agaagctgat tgccctggca aaaaaggttg cagcatcaaa cggcactgca attaaggaag
    16441 gatgctacgt tggaaccacc gggccaacct ttgaaacccc aaaggaatac cagtacttta
    16501 gaattattgg cggtgatgca gttggcatgt caaccgttcc ggaggtaatt gtagctcggc
    16561 acatgaatat accagttttt gccatctcga ttataaccga cctgggcgtt ccgggcaaaa
    16621 ttatcgaggt cagccatgag gatgttcaaa aaattggcaa cctctcggag cagaagatga
    16681 cccgaataat gaaagatatg cttacacagc tttaacaaga gctatgtttt atgcgaaaga
    16741 atcatggggc ggttagaggg gttacccctt ctgccccatg ctaattttta gctacaacaa
    16801 tttctcctcc tttacaattg ccgaaatcaa ctgggcaagt tcagcagggt taaggttgtc
    16861 tccatcaacc accagctgag ccttgttgta aaactttttc cgttcgtcga gcaccctgag
    16921 gatatagtta gccagctcat caccagtttt tccccaaagt aatgggcggt caatcttcga
    16981 ttcaattaaa cgggaaacca gtgtagccac ctccattcgc aggtaaacgg ttataccgtt
    17041 cttgttcata aagtcaatat tgttgtaaaa gcatgaagtg ccgccaccag ctgagatgac
    17101 atagtcgtct ttgtaatcac aaacctcctt aagggcttca ttttcaacct tccggaagta
    17161 gtcttctcct ttaaactcaa acagctgctt gatggtttgg ttatgcctgc tttcgataaa
    17221 atcatctaaa tctataaatt tatactgcag tgcgctggcc agctcccgac caatagtcgt
    17281 tttaccgctt gccataaatc caattaaaaa tattttcatg ttagtggttt tttaaatcaa
    17341 cctaatcctt caaaaattgc tgcctaaatc tctataaaaa aatacaaaac agttcaagtt
    17401 gaaaatataa gggttacaag ccgtctattt tacaagaaat gtgaaaaaaa ggttacaaat
    17461 tgcatggtgc taccctcccg cttggcattg tttacggtta taacgtttat tcgaacctgt
    17521 aattcaagcc ttatggaaac ttgcagctaa gactaccccc tcgctgagct tgttgtaacc
    17581 tcaaaaaatt tgattagatt ggcaatacaa tacaaatttt tgcgcaccaa cacctcaacc
    17641 taaaaacatt tcgtattttt aagcggatat ttagctggtg tgcctttaaa aatataaaac
    17701 ctttccggtt atgaaggtca agatgcgaat tggagtaaaa atgttactct ttgtcatgtc
    17761 aacctcggtg ctggtgatgc tggttattgg gcttttcatt ggttttcgaa taaataaaat
    17821 tgcccgggac gatgcggtaa gaatagttca atccgaggct cagaagaccg ccagccaggt
    17881 aaaatcggag ctggagctgg acatgggctt ttccagggcg ctggcacatg ccctgtacat
    17941 ctatcccaaa ttcgatacgc ttactctcga ctccatattc attaacatac tcaaaaatca
    18001 ggtagccaac aacccacgct acctcactgt ttggtactct attgagtact ccgcctaccg
    18061 accgggttac acaaagaatt acggcaggcg ttcggttacc gcctatctta gggatggtaa
    18121 tattggcgtt gatatcgagc ataagaacgt taccggtgat gttacaacaa gcaactacta
    18181 ctcctctaaa acatgcaact gtgaaatgct gctcgacccc tatacctttg tttatggggg
    18241 cagggaggtg ctggccacca gccttagcgt gccggtaaag gtaggcgatc ggtttgccgg
    18301 tttaggaggt gtagatatct cgttggagaa gtttcaggaa accatcgaaa aaattaaacc
    18361 ctacagcaac accagggtaa gtctgattgc cagtgacggg accattattg ccgatacgaa
    18421 ccccgaaaac gccaggctaa agttcagcga acttttccct tccgaagagg caacctacca
    18481 gctttcccaa aaaataaaaa atgggttgga ctttcagtac ctcacaattc aggacaacag
    18541 agaattttta aatattataa cccctgtaaa agttggagat tcgcctacct actggtcggt
    18601 ttgtctttcc attcccctcg atgtagtggt tgccgaggct agaaaagctg ttatttatac
    18661 aattctggta ttcctttttg gaatcattct gcaaaccctg gccatttggt atatttcaaa
    18721 ctacataacc cgcccgataa agaaaactac cggggtgctg aacaaccttg cagaaggcga
    18781 tattgatatc aacagcaaaa tatacattca tacgggtgac gagcttgagg aaatggcacg
    18841 ttccaccagc aagctgattg atgggctcaa ccatacagtt cagtttgccc gagaaattgg
    18901 tcagggcaag cttgatagcg aatttaccct gcttagcagt aaggacaggc ttggggaagc
    18961 tcttattgat atgcaaaaaa gcctgcttaa agccagggaa gcagaagtgc agaggaagga
    19021 tgaggaaaat aagcaaaact gggcaaccag gggtgttgcc atgtttggtg aggtgcttcg
    19081 gcagcataac gataacctga acgagctgtc ctacctcatc gtcaaaaacc tggtgagcta
    19141 taccggttcc attcagggcg gcatatacct gataaatgac aacgactccg ataacccgtt
    19201 cctcgaaatg accgcctgct atgcctacga ccgtagaaaa tttatggaaa aaaacattct
    19261 gcccaacgag ggattggtgg gcaggtgcta cgttgaggga aaaacaattt tgatgaccga
    19321 tgttcccaag aactacatta aaataacctc agggctgggc ggcgagaacc cccgatgcct
    19381 cctaattgtc cccttaaagg taaacgacac ggtgttgggg gttatagagt tggcctcatt
    19441 caacccttat cagaagtacc aggttgattt tatcgaaaaa atagccgaaa gcatagctgc
    19501 cacgctgtcg agtgttaggg tgaacatccg taccgccgag ctgctggaac gtacacaaca
    19561 gcaggcagag gaaatgaaag cgcaagagga ggaaatgagg caaaacatgg aggagctaca
    19621 cgccacccag gaggagatgg agcgtaaacg acttgagcag gaatccatta atacccagct
    19681 tgctgaggat agaacaatcc tcaacagcct gatgcagaat ttacccgact gcatgttcca
    19741 aaaggatgtt aacggccgct acgttaggct aagcaactcc atgctcaacc ttttaggaat
    19801 cgaccagccc gacgaggtta tagggttaac tgattttgac ctgtttccta cttcgctggc
    19861 gaagctactt tccaagctgg acagcgaagt gctgagctct cgtcaaccta ttaccagtaa
    19921 acccattgag ctggtgctta agaatggagt agaacaggat gccctgctca cgcttatgcc
    19981 cctggttgaa gagaatggag atgctattgg agttatgggc gtcatgaaac ttgcttaagc
    20041 ggaataaagc ggctcgagga attgagcatc aactccagca cctgtaaagc taggtgaaca
    20101 cctcatacac aaaaagaggc atcaaggaaa ttgcttacct gcctggcaga gtagattaaa
    20161 tagacaacct cactcgccct tagcgacgag gttttctgct tcaaccaaac ttcgaataaa
    20221 aaaactaagg atgttactag ctactagtgc ttggagttac cgtcgtatgc ccatttaatg
    20281 taagcagcac cccatgtaaa accagcgcca aacgtggcaa ttatgatgtt atcgcccttt
    20341 ttcagctgcg attcccactc ccagaggcag aggggaatgg ttgcactggt agtattcccg
    20401 tagcgctgaa tgttgatcat cacctgatcc ttactgatgc ccattctatg agctgtagcc
    20461 tctattatac gcatgttggc ctggtgtgga accagccatg ccaggttctc cggcttaatg
    20521 ttgtttcgtt ccataatctc aaccgacaca tctgccatgt tggaaactgc agctttaaaa
    20581 accggttgac cctcctgata aatgtagtgc tgacgttttg caacggtttc aaatgaagca
    20641 ggatagcatg aacctccagc cacctggtgc aggtgcttac ggccagaccc gtcaacctgc
    20701 atcttggcat ctagtagccc taacccgtcg tagttgggtt caagcagcac agctccggcc
    20761 ccgtcgccaa aaatgggaca tgtagcccgg tcggtatagt cggtaatgga cgacatctta
    20821 tcggctccaa ccacaaccac ctttttgtgc gttccggatt ctatgaattt agaagcagta
    20881 accaacgaat ataaaaagct ggagcatccg gcgttaatat cgtacccaaa ggaattcttg
    20941 atgccacact tgtcgctaat gatgttggca gttgcaggaa actgcatgtc gggtgtaacc
    21001 acgccgcaaa tcagcatatc aacctcctca ggccgggtgt tggttttctg aaggagttcg
    21061 tttactgcca taacacccat ttcggaggtg ccaaggcctt cacccttcag aatccttcgc
    21121 tccttaatgc caattcttgt cataatccac tcgtcggtag tatcaaccat tcggcttaac
    21181 tcatcgttgg tcaggatgta gtcggggaca taccctccca cacccgtgat tgcagctgtg
    21241 attttcacca tttgttaaaa gcttgtttaa ttttttcgca aaggtttgag gagataacct
    21301 cacgggtctg aagtatcatg ttcattatgg cctttgggct tgatgctccg tgggcaataa
    21361 caacgggttt gttaattccc agaacaggtg tgccaccgta attctctgag ttgaagcgct
    21421 cggtaaatga gctgttgggg ctgagttttt ttaaaagatg gtaaaaagcc tcggcttctt
    21481 ttagcactat atttccaaca aacccatcgc aaacaaccac atcggcctta tcggcatcga
    21541 agaggttatg cccctcaatg ttgccaacaa agttgaaatc cgccgaatcc ttcatttgct
    21601 cgaaagcact tttagtaacc aggttaccct tttcatcctc ctctcctacg ttaaggagtg
    21661 caatgcgtgg attttcgaca cccagcacct gcttggagta aattgaccca aggataccgt
    21721 actggtaaag gacatcaggc cggcagtcag ggttcagtcc cacatcaagc aacaggttta
    21781 cgctgttgtc cgaaacgggg ataaacccgg caatagcggg acgaataaca ccgggaattt
    21841 gtttaaccac aagcatggcg ccgaccatca tggcgccggt gctgccggca cttgcaaaac
    21901 catcgattgc tcccgcagca agagccgaaa accctctcac gatactggaa tcggtctttt
    21961 tagaaaaaga ttttgatgga ttatcgccca tggcaataac ctcggagcac ggctctatat
    22021 caaagcagtt gacgctacca ccctccagcg aattaatcag ctcctcaatt tttgccttgt
    22081 cgccaaacaa aacaatcctt tcatcagcct taagctgttt gcaggctaga atagaacccc
    22141 ttacaacagc ttcgggggca aaatcaccgc ccatcgcatc aacgccaatt ctcatcgttg
    22201 tggttttggt tgaatacgag atgactacta ggcgctgaca gatttttcaa ttgccagctt
    22261 gccacggtag tatccacact ccgggcatac cctgtggtac tccacagctg caccacagtt
    22321 tgaacaagta gaaagagttg gcaattctgc cttgtagtgg gttctacgct tattcctcct
    22381 ctgtttggaa atttttgatt taggatgtgc cattttgtga aatatttaag ttactgttgt
    22441 tgatacttag ttacttgtta agcttttgag cttgtcccaa cgaggatcaa cctcctgatt
    22501 ttcgtgccca tgagaaccaa gcaatgatac catatcgcta tcgcagtcgg aagcgcttgt
    22561 tccgttcatc ccgtggtatc gttctattgg catactcagg tatatactct cgtaaagata
    22621 ttgtgccagg ttaacctcaa agtcattttc ggaaaggtag ataatctcat cggaatctga
    22681 ttcctggctg gcgtcaatct ttacaattag cttgccgtca aattcaacag gaaaattaaa
    22741 ctgttcaagg cacctatcgc aggcaatctc caccataccc tcaatgtggg tgctaaactc
    22801 aagtaatctc gattgcttgt tgaggcttac cctggcaacc agctcgccgt tatggatttc
    22861 cccctcctca aatgcgctga aaaaactcct tcccagctgg aaactaaaca gatgctttcc
    22921 cagcttaagt cccttaaaat taattgaata tgcatcgagc acgtttgcca ccttacccaa
    22981 taaaattagg ttgcaaattt ataaaattaa atatcaaacg tataaataat ggctgaaaat
    23041 taggttggtg gcttcaccta aagggaaagt tcgggttatc agaaattatg caaccctggc
    23101 gatacgcttg cgctgggttt cgtcaagaat aattttgcgt agccgcatgg ctttcggggt
    23161 tacctcaacg tactcatctt cctgaatgta ttctaatgct tcctcaaggc taaaccgaat
    23221 ggggggagcc aggaagattt tttcgtccga accggcagct cgcatgttgg tgagcttctt
    23281 cgacttggta acattcacaa ccaaatcgcc agagcggttg tgctctccaa ctacctgtcc
    23341 ggcataaacc tcctcatggt catcaatgaa aaacctaccc ctatcctgta gcttgttaag
    23401 ggcataggcg tatgaaattc cgctctccat tgccaccagg gagccgttag tacgggtctc
    23461 gaaatcaccc cggtagggtt caaacccttt cacccggtgg gcaataacag cctcaccggc
    23521 agtggcggta agcaggatgc ttcgtagtcc aatgatgcca cgtgaaggaa tttcaaactc
    23581 aaggtgaatc ctgtcgcctc gtcgctccat aagcaacagg gcgcccttcc tgcgggtaac
    23641 ctgttctatt gccttgcccg acacatcctc cggtaggtcg atagtcaggt actcaatggg
    23701 ttcgcacttc tcaccaccaa ccattttgat gataaccttc ggctgaccaa cctgaagctc
    23761 gtatccttcc cggcgcatgg tctcaataag caccgatagg tgtagcactc cccgtccaaa
    23821 caccatgaat gaatcggccg agtcggtttt ctcaaccctt agggcgagat ttttttccag
    23881 ctccctgtcg agccgctctt tcaaatggcg ggaggttaca aacttgccat ccctgccaaa
    23941 gaaaggagag ttgttgatgg tgaagagcat gctcatggtt ggctcgtcaa tggcaatggt
    24001 tggaagtggc tcaggattct cataatcggc aatggtatcg ccaatatcaa agccctcaat
    24061 gcctacaaga gcacagatgt cgccgcattc cacccgttcg acactatttt tttcaagtcc
    24121 ctcaaaaacc aacaggtcct tgattttagt tcgaactacc tcaccgttcc tcttaaccag
    24181 cgatataggc tgccctgcct gcaggctgcc ccggtgtagc ttccctatgg caattcggcc
    24241 cacatagcta gagtagtcaa gcgatgtaac cagaagctgg gttgttcctt cggtcacttt
    24301 tggcgcaggc acgtagtcaa taatgcagtc gagcagcggg gtaatatctt tcgagggttt
    24361 ttgccagtcg ttgctcatcc atccctgctt ggcgctcccg taaatagttg gaaagttgag
    24421 ctgctcctcg gtggcatcaa ggttgtacat cagctcaaaa acctgttcct gtacctctgc
    24481 cggacggcag ttgggtttgt caaccttgtt tatcaccact attggcttaa gccctatggc
    24541 cagcgctttc tgaagcacaa aacgggtttg gggcatggtt ccctcaaaag catcaaccag
    24601 cagcaaaacc ccatcggcca tgttgagcac ccgctccacc tcacccccaa aatcggagtg
    24661 cccaggcgta tcaatgatgt ttatcttgta acccttgtag tttaccgaaa gattttttgc
    24721 cagaatggtg atgccccgct cccgctccag atcgttgctg tccagtataa gctcgcctgg
    24781 cctttcgtaa tccttaaaaa gtttcccctg aaaaatcatt ttgtccacca gcgtggtctt
    24841 cccatgatcc acatgcgcaa ttatcgcaat gtttcgtatc ttctgcatct aaaaaatttt
    24901 aaccggcaaa attaattcta tttatttaat ttttccccca cgctgaatat aaaaagtaag
    24961 gcatgtagtt gttttgaaag ttttacccac taattttggt tatttgtcaa atagttcatt
    25021 ttttctgaat ggttaagcgt atcgataaat aaaaggaaaa aataagattt tattttgaaa
    25081 cataatttga caattaatct agcgtaacgt aacttgtatt tttttaaatc tttaagtatc
    25141 aacctgcact aaaaagagat gtggctggaa gatttctact ttcttgtttc ttgatatata
    25201 tagacaattt gttagtatca ggccttacat attttaccaa ataacgcatt aaacagaagc
    25261 tatgagtata acttttagta accttttggt ataggcaggt tctgctcatg gctaattcct
    25321 aaccctaatt aaacccagac aaataaattt aagcagatga aaacaaaact tttatgcttt
    25381 atccttttgt tagccgtaat tcctgtcctt ggacaggtaa cacctcaagc gccgcagaaa
    25441 ttttcctacc aggctgttct gcgaaatgcc gatggtacgg ttattggcaa tcaaagcgtt
    25501 gaggtgcagg tgaagcttca tgcgcaagct gccgacgggg cagtggtgta cactcaaact
    25561 caaaccgttg agaccaacca gtttggcctg gtagcttttg cagttggcgg tagcagcgga
    25621 tttgaaacca ttccttggac cgatccaata ttcatggaga tccttgtaaa gagatcgggc
    25681 gaatccagct tttcctccat gggaactacc cagattatct cggttcccta tgcccttgtt
    25741 gccaacaacg gtattcaggg gccttacatt cagggtcaaa ccgttttcag caatggcggg
    25801 ggttgggtcg gcactaaccg agtgatggtg gccgacagct cggtggttgt tactacaccc
    25861 cttggccacg actcatcaaa gccaattttt gccgttacca acgccaatgg agaaatggtg
    25921 atggcggttt acgaatcggg cgtgaggttt tatgttgaag aggaaactac caagggagta
    25981 aagggtggat ttgccgtggg cggtctcacc aaccaggtga aagaaacaaa aagcggtgag
    26041 cctatttact acatgaacat tcagccaaca caggttcann nnnnnnnnnn nnnnnnnnnn
    26101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    26161 nnnnnnnnnn nnnnnnnnaa acaaaaagcg gtgagcctat ttactacatg aacattcagc
    26221 caacacaggt tcagttcctg gtagaggaac cagctgtcaa gggagtaaag ggtggatttg
    26281 ccgttggcgg gctcaccaac cagggaaaga cgttaactcc tactgaaaac tactttgagg
    26341 ttaacaggga ctccgcccaa ttccttactc catcgctggt atctcagggt aacataacca
    26401 ctcagggtaa cattactgtt agcggaaaca ttaccaccaa cgttgggcaa gctgattcca
    26461 cactgaccga tgttgacggc aacacctatc agacagttcg cattggattc cagcagtgga
    26521 tggccgaaaa tcttaaaaca acgcactatt ctgatggaac gccgattgac accgccgtcc
    26581 atgtctataa cgactgggct taccccgact ccatcaacat atacggcagg ctctacagcg
    26641 gactggctat tagcagcggt aataacgttt gccccgatgg ttggcatgtt ccaactcctg
    26701 acgactggaa tgagctgttt acattcattg ccggtcctgc agggatttcg gatatgcaat
    26761 ccagggttct tgttctcaaa aaattgttag agccaggcac catgatggaa ggaaacggct
    26821 actggaatta ccccatatac cccaccaacg aaacggggtt cactgccagg cctgcaggac
    26881 aaagagacta cctgacctac tattccgaac tgcacgacta tgcctacttc tggtcatacg
    26941 ttgtgggtgt tgggttgcaa caggtttcca tgacaacaat ggatggaggt aatctagaaa
    27001 ttatgccacc atcagatcct aattactcat tctcagttag atgcttgcaa aatccttaaa
    27061 aagagaattt ttagcaacag gctagctctt ctaacttaga acaacttgcg ctctataaag
    27121 tatagctaac ttgctgctca gctggcaaag atcaagggtc ggagcaaggt ttactccgac
    27181 ccctattttt tgccctgcta aattttcccc agaatcccac ttgccacatc catgctacat
    27241 attcagtaat ccccaaacgc cggccaggtt taacaagcaa aaactcttgc ctacccgttt
    27301 catccgacat tatgtacaga ctcaacaaaa ttgttcaacc catgttacac atagagttga
    27361 aaatttagcg catttttgtc gttgattttt aaactataac taaggggcaa acctgctaaa
    27421 cactttaggt ttcagctgta agctggtggt gaacaggctg ccatgcggat acaaaataag
    27481 cacccacagg atgaacaaaa aaaaggttat tatagctggt ggggggtttg ccggaattca
    27541 gcttgcccga aagctcgacc agcggctatt cgacgtgttg ttggtagata aaatcaacca
    27601 ccaccagttt cagccgcttt tctaccaggt tgccacctcg caacttgaac catcaagtat
    27661 ttccttccca ttaaggaaca tctttagcag gaaaaaaaat gttcagatta gactagctga
    27721 aatcctctcc attgatcgaa atgagagcaa aatcattacc agtataggcg aatttagcta
    27781 cgattacctg gtgctggcca tgggctgcac caacaacttt ttcggaaatg aaaacattaa
    27841 aaaaaatgct ttcactctaa aaacaaccta cgatgccatt accatcagaa accacatcat
    27901 tctaacattc gaaaaaattg tatcggctaa ggaggacgag ctcgaagcgc ttaacaacct
    27961 tgcaatagtt ggtgccggcc caacaggagt tgaacttgcc ggcgcatttg ccgaaataaa
    28021 aaagaatatc ctgccaaagg attacgcagg tgttgacttt tcaaaattca acataattct
    28081 aatcgagggt acccgaaaca cgcttggcag catgagcgat gcctcaaagg tggagtcaag
    28141 aaggtaccta cagcaaatgg gcgttacaat actgacagaa actttcgtag ccgactacga
    28201 cggatatcta ttaaagctga gcaacggaga aaccataaaa acaaaaacgg ttatctgggt
    28261 tgcaggagtt accggaaaca acatcgaagg gctcccggaa gctgcaaggg ctcccggaaa
    28321 caggataagc gttgacaggt ttaaccgggt taacggatgc gataatgtat tcgcagtagg
    28381 cgacatagcc tacatggtaa cccccaagta ccccaaaggg cacccacagg ttgccaacgt
    28441 ggccattaac caggcaaaac ttctggcaaa aaatctaaaa tccattcaaa caggcaaaaa
    28501 gctaaaggcg tatgagtata aaaatttggg gaccatggcc acaatcggcc ggaataaggc
    28561 tgtggttgac ctaccccaca ttcatcttaa gggttatttt gcatggttca tctggatgtc
    28621 gcttcacctc atgctcattc ttagcgtgag aaacaagctg ataattttta tcaactgggc
    28681 atggtcgtac ctgaccaaag atacctcgct ccgattaatc ctcaaggaag ataagccccg
    28741 aaccaataca tagctaagta gaattcatat tctcactaaa ccttcaggaa cactattgca
    28801 tttccgccaa ccgttcaaaa acactactgt agaaactcaa atccacaaga tacccgtggc
    28861 cgatgctcta aattaatcta tagaacatct taagcgcatg cgaaggggaa aaggaatttt
    28921 ctggatgaaa aatcattccc tctaacctag agcggtcaac taaaaatcct gccgccatat
    28981 ttaatcatgc tgaatatctg caacatacag cacggataac atcaaaagat atttcaattc
    29041 ctttaaaatt tgttgccgtt acctgccaat tgatttactt tgtgcaaatt ttttcatcca
    29101 tatattatta aggtatgatt caaaacctgg taatagttga gtcccccgca aaggccaaaa
    29161 ccattgagaa gtttttgggc aaggagtacg tggtaaaatc gagctttggg catattcgcg
    29221 acctatccaa aaagaattta ggtgtagata tcgccaaaaa ctacgctccg gagtacattg
    29281 taagcgacga caagaaaaaa gttgttgatg agcttaaaaa agccacttcc gaagcacaga
    29341 tggtttggct agcttccgat gaggaccgtg agggagaggc aattgcctgg cacctggctg
    29401 aggttttaaa cctcaaccct aagaaaacca agcggattgt gtttcatgaa atcacaaaaa
    29461 gcgccatact aaatgccatt gaaacacccc gcagcataga cctggacctt gtgaattcgc
    29521 aacaagcccg aagggtgctg gaccggctgg taggctttga actttcgcca attctctgga
    29581 aaaaggttaa gcccgccctt tcagccggaa gggtgcaatc ggttgccgta aggctaattg
    29641 tggaacgtga acgcgagata attgccttca caccaacacc atactacaga gtaacagcca
    29701 tattcatggc cgactcggaa tttaacgccg aactatcggt taggctatca actttcgagg
    29761 aagcattatc cctgctggag cattgcaaaa atgccacctt cacagtgcag gatgttacca
    29821 aaaaaccctc aaaaaaggca cccgctccac cattcaccac ctccaccttg caacaggagg
    29881 ctagtcgtaa atatggtttt tctgttgcgc aaacaatgtc gatagcccag aaactttacg
    29941 aagcaggcca cataacctac atgcgtaccg actctgtaaa cctttcttca acagctattg
    30001 ctgccgcaag agatgtaata gtagcagaat acggtgagaa ataccatcaa cccagacagt
    30061 acaaaaccaa aaccaaaggt gcccaggaag cgcatgaagc cataaggcca acctacctcg
    30121 acagggttaa gataagcgga accagcaccg agcaaaaact ctacgattta atcaggaaac
    30181 gaaccatagc ctcccagatg agcgatgccc aaattgagaa aactactata aacattgata
    30241 tatcgggatc gcattacaaa tttgttgctt ccggcgaagt aatcgtgttc gacggatttc
    30301 tcaaggttta ccttgaatcg tccgatgacg aagccgagga aaacggacgt gaaattcttc
    30361 ccccggtaaa ggccggccaa accctagcgg taatggaaat tgttgccaac gaacgtttta
    30421 cccaacgtcc cccaaggtac accgaggcca gcttggttaa gaagctcgaa gagctgggta
    30481 ttggcagacc atcaacctac gcaccaacta tttccacaat catttcccgc ggctatgtgg
    30541 ccaaggaaga cagaccggga gttgagcgaa actataagtc cataatcctt aaaaatggta
    30601 aaataacccg gcaggaaaag gttgaaaact ccggtgccga aaaagccaaa ctttttccaa
    30661 gtgatatcgg tatggtggtt acagatttcc tacttgagta cttcagggat atcctcagct
    30721 acgattttac cgcccacatc gaggagaagt ttgatgaggt tgcccttggc aaactcgagt
    30781 ggacaagaat gatcgacgaa ttctataaac cctttcactc ccttgttgaa aaaactatca
    30841 aggagtcgga ttacactaag ggtgaacgtg tgcttggcat tgatccggtt agcggtaaac
    30901 ctgtgagcgt ccgtataggc cgatatggcc cggtggttca gctgggagag gctgacgctt
    30961 caaaaaaaac aaaaccacag ttcgccagct tactaaaagg acagctgata gaaactatta
    31021 cccttgagga agcattgtcg ctcttcaagc tgcctcgttc ccttggaaac tacgaaaact
    31081 ctgaggttgt tgttggcgtt ggaagatttg gaccctatgt tcgacatcaa tccaaatttt
    31141 actcgctcaa aaaggacgat aaccccctca ccattgatct caatagagcg gttgagctga
    31201 ttgaagaagg acgtaaaaaa gaacgcgaaa gggttatcaa aaagttcgaa tcccctcaag
    31261 tagttgaaat cctaaatggt agatggggcc catacatttc attcaacaag cagaactaca
    31321 aaatacccaa ggacaaagat ccgaaattgc ttaccttcga tgactgcatg aagctcattg
    31381 acgaacaggc tgaaaaagga agtaaaagga attctactgc caaaaaaact ttgcccaagc
    31441 ggcaggggaa aaaaggaaac acaaaatagt ttgccatttg gcgccaccat ataactttta
    31501 aaaccatgga cgagctgctg tcatttttaa cacccacagc cattaagggg gttaacttta
    31561 gctcccaaaa cagccgtatg ctccagcaac aagtagtccc gcttaacaaa actaactaca
    31621 agcacctaaa aaaaaatgat atagttgtaa tcggcgtaaa tgaaagccga aactccccaa
    31681 atccatctat caaaaactcc cctgaaacca ttagaaagta cctttatggg ctttcgacca
    31741 taaagctagc cggtaaaata tatgatgcag gcgatctggt ggaaaccagc acccccttaa
    31801 aaacctatga ggccttaaat cgagttgtag aaattctatt ccagaccgga gcagcaacag
    31861 tggtattggg cggaacccag gagttaactt actgccttta cgaaggcgta aagtctaaca
    31921 aatcgctggt aacaatttcc attatcgatt gtcttattga tgccgacgaa gacttgtctg
    31981 attttcattc acgtaactat gtcaacaggc tgctgggcga aaagctgcgc tcgcttttcg
    32041 aattaagcat tgttggctac cagggctact atgttggcgg agatatggtt aacctgtttc
    32101 agaaaaacaa tcacgaactt ttacggcttg gctatgtcag gagtagctat cgcgaggttg
    32161 agccaaccct tcgaaatgcc gacattgtaa gcttcgacat ggaagccata agggcctgcg
    32221 actctcctgc caacaccatt ccctctccta atgggctcta cgctgaggaa gcttgtcaac
    32281 ttgcccatta tgcaggtgtt ggagaacgag ctagcctttt cggaattttc aactacaatc
    32341 ctgatacgga caacacagaa cagtcggcct tacttgcagc ccagctagca tggcacttca
    32401 ttgagggtta ttcaaaaagg aaaaacgttc ccaaaaccct aaatacacct gactacctga
    32461 agtttatcgt aggaactggc acatcaggag ttgacatggt tttctactgc gacgaagcgc
    32521 tcgatcgctg gtggatggag attcctattt cccctgaaat gggcattccc accaatagaa
    32581 ttattatccc atgctcccat agcgattaca ccctggcaag ccgacaggag ctgcccgacc
    32641 ggtggctccg aatatttcag aaaatacagc agctctagtg cagctcatct tttgctttag
    32701 ggttaagctg tagctgctat tgttaaactt gaccctactt ttctatattt ttgaattata
    32761 cggtaaccct tttggtcaaa aatctgtata acattctgcc ttcggttaaa attttatcat
    32821 atttgttgat aaatttgtat gcctctaatt tttttgctta ctttactatc taattgaaaa
    32881 gaacgttgaa tattttttat gctttttata tatagctatg catagctcat cccgaaaatt
    32941 tctgttattc cagctgtttc tcccttttgc gggcattcta atggctcagc aggatccaca
    33001 atttagccaa attctgtata atccaatgag catcaacccc ggatatgctg gcagccagga
    33061 aatgatagga ataggggcat ttaacaagct tcaatggact ggttttggcg atggggctcc
    33121 tgttagcact actgttagca ttaatgcccc catcgcacca tttggtttta aaagcggttt
    33181 ggggttgagc atactaaacg accagtacgg atttaacagc gacctgggca tcagcttagc
    33241 ctatgccgca cggttcaaac caaaactcat aaacggtagc cttggaattg gagtaagcgg
    33301 aggtttcgta aacaacacca ttgatccaaa gtggaactat ccggttgcag gaaacgacca
    33361 ggcaattccc caacagaaag aaagttctgt gaaccttgac cttgggctag gccttttcta
    33421 caccaccgac tatatgttct tcggcgtttc ggcaacccat attaaccggg ctaaaatcaa
    33481 caaaagcccc atacctgcac gctatacccc tcagtactac atttccggag gttacatcgt
    33541 tatgttccca tctaaaacct ggcaatttga accctcagta ctgatagtaa ccgacattaa
    33601 atcgtcaaga ttatccctaa attcaattgt tcggtacaat aaaaagtttt ggggcggcgt
    33661 ttcataccgt ataaatgagg cagttaccgg aatgattgga tttgaactct tcaacggatt
    33721 ccgaataggt tatgcctacg acttcagcct tagccggata agtaagtata atgacggatc
    33781 gcacgagttc atgctgggtt acagttttca gcttaagaag gagaaaccgc cccagcagta
    33841 taaaagtata agatttttat aatttttttt tcatgagttt aaaaaatttg cgttatttgc
    33901 taaataaata tggcagtatg aaaaaactcc ttcctatagg cattgcagta attctgcttt
    33961 ccagctgttc tccaggagga aatggcgagt taacgggagt acccggtcgt agaggcttct
    34021 ctgaaccaac cccctatggc atggtattta tcccaatggg atcgtttaca attggtagta
    34081 acgaccagga tgttgcgtgg gccatcaatt cacccactaa aaccgtgtcg gttgatgcat
    34141 tttggatgga ccaaaccgaa ataaccaaca accagtaccg ccagtttgtg tactatgttc
    34201 gcgactccat agccaggcgt atgctggcag aggttaacga tgaatttgtc attaccgagg
    34261 atgaatttgg caaccccata gatcctcctg ttctaaactg ggaagtacca attgacccca
    34321 acgacgagga gcagatggaa gtgcttaata acatgtacta ctctaaagag gatgcctttt
    34381 acggaagaaa ggaaatcgat acccgcaagc tcttttacga gtactactgg attgacatga
    34441 agcaggccgg tcagaaaaac aaccggtata actttgaaac actaagctac gataaaggag
    34501 aggtggtcaa cagcctggga gagaaggtta aggtggataa ccgccaaagt ttcatcatgc
    34561 gcgacatggt taatgtttac cccgacacgc tatgctggat ttccgatttt tcttacgctt
    34621 tcaacgaacc ttatgcaaac agctactttt ggcatccggc attcgacaac tatcctgttg
    34681 taggcgtaac ctggaagcag gctacagctt tctgcatatg gcgaacccaa cttcttaaca
    34741 agtaccttgc ccctgaagga attaccattc aggattacag gctccccagc gaagcagaat
    34801 gggagtatgc cgcacgcggt gatcttgatc atagcatgta tccttggggt ggcccctaca
    34861 cccgtaacat agagggctgc tttattgcaa actttaaacc cctcagagga aactacattg
    34921 atgacggcgg attaattccc atcgcagctg gtagctatga acctaatgac tacggactat
    34981 acgacatggc cggcaacgtt gccgaatgga cagcaaacgc tttcgacgaa agcacctatt
    35041 ccttcatgca cgatatgaat cccgactata agtacaatgc taaacctaac gatttgcctg
    35101 ccatgaagcg taaggtaatt aggggcggct cctggaagga tatcagcttc tttcttcagg
    35161 ttggctccag agcatttgag taccaggatt ctgcaaaatc gtatattgga tttcgttgcg
    35221 tgaggtcata ccttggtgct aagtaacctg aacatgtagg tttttaaaat cataaattcc
    35281 tttagcatga aaatcaacat tgaagaactc gttacctccc ggaagtggaa aaccttcatg
    35341 aaatacctct acggttgggg tgcatcggtg gttatccttg gagctctctt taaaatactg
    35401 cacctcccgg gcgcaggtac tatgcttatg gcagggatgt gcaccgaatc aattatattc
    35461 tttttctccg catttgaacc actgcccgat gagtttgact ggacacttgt gtttcctgag
    35521 ctaactggta tgacagagga ggacgaaata gacggctaca agagttccag acgccagggc
    35581 attggtgccg aggagataca tgaaatcgtt accggtattc ttgcctccat gcctgcagga
    35641 agtgttccgg cagctcaggc tattgccccc gagaaagttg aaactacacc cccgcaccag
    35701 gcagctgcca ccgcagcccc gcagatctcg gtgcaacatg ctggagcagg agctggcggt
    35761 gccctcatct ttaccgagaa gtttaaccaa atgctcgaga atgctgagat tagcccagcc
    35821 ctattcgata aggtaagcca aggacttaat aagctaagcc aggcatcaag tagcattgca
    35881 gatattacca caactgccgt tcatgttaag gagtttaccg ataacatggc caaagcttct
    35941 gaatcagtta aaacattcca cgagaactac tcacagaata gccaggttct caacgaatcc
    36001 atgaatcacc tttcggacag cattcagaaa accgccggcg aaatgtccga atcgggcaag
    36061 aactttatgg atggagttgc cggttcggtt cagaatttgg agaatgagct tactgctgca
    36121 ggtcaaaaag tagggggaag gattgtggag tcatgcgaga ccgttgccgg caaactcaac
    36181 atggctgcag aggggctggc caacgcctac cagcaaacag ccgaaggggt tgcgataact
    36241 taccagcagc tggccgaggc aatgaagaat aacggttcaa ccataaccaa gggtagcact
    36301 acctacaacc agcagctgga gcaactaaat aagaatatgg cggcccttaa cacggttcat
    36361 gagatgcacc ttcaggaaac atcccaacgg ttgaaggaag ccgaaaagct gtaccagggg
    36421 gtggatggaa tgataaagaa gttgaatgtt accgttgatg aatccgaaaa gttcaaatca
    36481 gcactggaaa cacttaacag caatatcacg tctctaaata atgtttatgg caacatgctg
    36541 gcggctatca actcgatttc caccagtaag taaatcaact gcatcttaag actcacctaa
    36601 cggtctatgg cacacggaaa agaaactccc aggcaaaaaa tgatcggcat gatgtacctt
    36661 gtgctgaccg ctatgcttgc gctgaatgtc tcaactgatg ttctggaggc ttttgtactg
    36721 gttgacaacg ggttatcgcg caccactgaa aactacaggc tcaagaacga taaactctac
    36781 tccgagtttg acaaggccat gctggttaac cccgtaaaag tcggcccttg gaaaagcaaa
    36841 gcggatgaga taaaagaaat ggccagggag atatacgact acgttcagga tctcaaaatt
    36901 gaggtggtta aaacatccga aggagatgat acacctgcaa ttaacggtag agaggtcata
    36961 agcgagttga tagaggataa agccaacctg gatataggag ggcagatcct aatcggcatg
    37021 gagggcaacg gcaaggcttt tgagctgaag cgtaaaataa ttaagtttcg tgaaaaagcc
    37081 atttccctac tcgaccccgt tcgtggtgaa gaaatgataa aatccatcaa tagcattcta
    37141 aacactgacg atccccccgt aagggaagat ggaactcaca actcatggga aagttctcgt
    37201 tttgagcata tacctctgat ttccgtaatt ccccagctta ccaaggttca ggttgatata
    37261 ctaaacaccg aggccgaagt ggtaaactac ctgctgcagc aaattggtgc atcggatttt
    37321 aagtttaacg tgctttcggc aacggtgatt ccaaactcca acgtggtatt tcagggtagc
    37381 gattttaagg cagaggtttt cctcgccgca tccgatacta cccaaaaacc aaaaatatac
    37441 ctttgccgtt acgactccac tcaaaaccct gtaactaagg agtggaccta caacccaatt
    37501 gggccgcagg aggaaatccc agtatcccaa aacggtagag gattgtattc ccgaagggct
    37561 ggagcaatag gacaaacaag ctggtcgggg cttattgaaa tgaaggctcc cgatggttca
    37621 attgtcagga aacctttcaa gcatacctac tccgttcttc agcccaacgt agttattgct
    37681 ccaaccaaaa tgaacgttct ttacctgggt gttgacaacc ctgtggatat ctcaatacct
    37741 ggagtaccac ccgataaggt taccgcttca ataagcaagg ggtccattag gcctgaaggt
    37801 aagggaagct acatcgtaca acccggagca ggctcctcaa catgtgaaat taccgtttac
    37861 gccaatcttg aagggagaag acaaagtatg ggaaccaaaa cattccgcat taaaaaggtt
    37921 ccaccacctc tccccagaat aattggtatt accggaaaat atgccgcgaa aaaccagctg
    37981 gctgcatcgt tgggcatagc tgccgaaatg ccccaggact tcgacttcga ccttaagtat
    38041 aagattgtta gcttcaggct ttcggcaaca gttggtggtt tccttcagga aaaagagtca
    38101 aacggttcaa ggtttacaga tgagcagaaa agaattgtaa gcaacctgcg gagcggaagc
    38161 cagctagcca ttaccgacat aaaagcggtt ggaccgagcg gtgatgttat tgacctgaac
    38221 gatatggtgt taaaaataag gtagtttcag ctattccatt ttgcaatgga taatgcgcat
    38281 taaacatttt tcatttacca gcatggttga aaataaatac taataaaatt acagggatga
    38341 aaaggatagt tgtgttattt ggtggatttt ttctcatact gggaacgctc catgccccag
    38401 cccaggaccg caaggaaagc ctggtaaacg atggttttta tacccgggaa actatccagc
    38461 agcgaaaacc cataccctac cagtatgttc gtgaatcaga tgttctttgg tcaaaacgta
    38521 tttggcggat ggttgacctg cgccaaaaaa tgaactaccc cctttacttc cccaccactc
    38581 caatgcagga cagggaaagc ctcgttcaac gcttggtaag ggcaattaag tacaacgaaa
    38641 ttaccgccta cgatccggtt gacgatgagt ttactacccg gatgtcctac gagcaggtta
    38701 tcaaaaacct tggcgccgag gatagggttg aaaaacagat tgacagcacc ggaaaggaga
    38761 cggatgtaac cataaagggt gaaatcaggt ggaacagcat taaggagctg ctgataaaag
    38821 aagactggtt ttttgacaag caaacatcaa ccatgcaggt tcgtatagtt ggaatatgcc
    38881 caattatgca tgatatgcga attttacaaa caggcgagga ggatcaggaa gaaaccggcg
    38941 aaataaccaa aaagcaaact ttctgggttt acttccccga agctcgcacc atactggcta
    39001 atactgattt tttcaattct tttaatgatg cccagcggat ttcgtttgat gacctctttt
    39061 ttaaacgcca gttcgattcc tacatcataa gggaatccaa cgtgtttgac aaccggtcga
    39121 tttccgatta cacctttggc ggtgtgcaga ccatgcagga atcggaaaaa attaaaaccg
    39181 acatattcaa catggagcat gatttatggg agtactagca tacttctgtt cttgttataa
    39241 aaggaaaagg ctgtcgtgcg gatagccttt tgcttttact atacttttct gaaattggct
    39301 aggtatatat ttctccatcc ttcagcatcg ccacatcgtt caaaatgttc ttaatttgct
    39361 gttcttcctg cttgcggcat ataagcaaaa catcgctggt atcgaccaca atatagtcgt
    39421 caagcccctg aacaactgcc agctttccat cgggagcaga aatcagcgag ttactggtat
    39481 cgtaaagtaa aacatttctg gcaatcaccg cattgccatt ttggttgacg ggaagatggg
    39541 tgtagagcga accccatgta cccaaatcgg accaaccaaa ttccgagcaa atcacgaaaa
    39601 cattttcggc tttctccata accccatagt caatggagat attcttgcac tcggcataag
    39661 ttttctcaat ggctgcggtc tcatcagccg aatcgagcaa cgaagcgacc tcgctgaaca
    39721 aggaattcac ctctggaaga tgctcttcaa gcgcttgggt aatggcattc agattccaaa
    39781 taaaaagtcc cgaattccaa aaaaactccc cgctgcttaa aaatactttg gccatttcca
    39841 ggtttggctt ctcggtgaaa gttttaaccc ggtatagccc atttatcccc ttaaccggtt
    39901 tacccacctg aatgtagcca tagccagttt caggcctgtt aggctttacg cccagggtaa
    39961 gcaaggcatt attttgcgat gcaaaatcca acgccaggtt aaccgtgtta gtaaaaacct
    40021 gctcgtttag aatgaggtgg tcactgggcg aaacaataac cgtagcttca gggttacgct
    40081 tgcgaatttt ataggtagca taagctatac atggtgccgt gttcctcctc ataggctcaa
    40141 gtaaaagctg gctctccttc acctcgggaa gctgctccaa gattaaattc ttgtaggaat
    40201 tacttgccac aataaaaata ttttcaacag ggcaaatttt taccatcctt tcaaacgtct
    40261 gctggagcag cgatttaccc acgccgagta tatcaataaa ctgttttgga catgcagttc
    40321 tgcttaatgg ccaaaaacgg cttccaattc ctccggccat aactatacag tagtaattct
    40381 tattactcat tgctttttat taaaaataca aacctacgga attttaacat gagatggaaa
    40441 ctttcggctt aaggtaatga aaggaaaacc ctacactaac cagaaaggaa gggcatttcg
    40501 agcaaactta acaaccaaca ccggaataaa cttaaccagt gcttaccatg gttaagcaaa
    40561 tggagcgata accctaacca ggatactgga ggaaatggac ggctgtgaat gtggcatgat
    40621 taattttact accatcttaa tattgactaa ttttgctgtt ggtagaaata cgatttctgc
    40681 caatccgatt aatttttgta agtaaaaagg tcaaagatgg taagaatatt cgggttaatt
    40741 ggggagtatt ttcttttgct gaaaaaggtt ttttcaaagc cggagaaggg aaggatttac
    40801 cttaaacaga ccataaagga gatggataag ctgggcattc aatccatcgg gattgttacc
    40861 ataatatcgg tttttatggg agcggtaata accctacaaa ccgcatataa catggaaaac
    40921 ccttttattc cccgctacat gattgggttg ggcacgcgcg acagcatgtt tctggagttc
    40981 tcttcaacaa tcattggcct aatacttgcc ggcaaggtgg gttccagcat agcatctgaa
    41041 ctaggcacca tgagggtaac cgagcaaatt gatgccctcg aaatcatggg tgttaactcc
    41101 gccagctacc tgattcttcc taaaattatt gccaccctta tttttaaccc ttttctttgc
    41161 atgctaagca tggtggtcgg gatagccgga ggatggatgg ttggagaatt ttcaggagta
    41221 atatctacac aagcctacat ttacggtata aggtatgcgt ttatcccctt ttacatcacc
    41281 tactcgctaa taaaaaccgt ggtatttgcc ttcctaatca ccactatttc tgcattttat
    41341 ggttaccgcg ttcagggcgg gtcgctcgag gtgggccagg caagtaccaa tgcggttgtc
    41401 gtcagcagcg tctttattct cctgtttaac ctaattctaa cccaactgct actgtcgtga
    41461 ttacggttaa aaatctgtca aaatcgtttg atggccgaat ggtgattgac aatataacgg
    41521 ccacgtttga aaagggaaaa accaacatgg taatcggcca aagcggttcg ggaaaaaccg
    41581 tcctgctgaa gtgcatagta ggcctgctgg atgtggatga gggagaaatt tactacaacg
    41641 acattcagtt taacgccctt tcgttcaaaa agaaaaaaga aatacgacaa aaaataggca
    41701 tgctttttca gggggcagcg ctattcgact cgcaaaccgt tgaggaaaat gtgatgtttc
    41761 ctcttaacat gttcaccaac atggccattg cggagaaaaa ggagcgggtg gccttctgcc
    41821 tcaaacgggt agatattgtt ggggccaaca agctttaccc ggctgagatt agcggcggca
    41881 tgaaaaagag ggtagccatt gcccgcgcca tcgttttaaa ccccgagttt ctgttctgcg
    41941 atgagccaaa ctcaggactt gaccccaaaa ctgcagtggt aattgaccat ctcattaagg
    42001 aaatcaccga agagtatggt attactacca tcataaatac gcacgacatg aactccgtga
    42061 tgggctgcgg ggataaggtg atgttcatct acaacggtaa aaaatggtgg gaaggaacca
    42121 ggttcgacgt gttggatagc gacaatcagg aactcaatga ctttgttttt gcctccgcac
    42181 ttacccgcaa aataaaagga atgtagcaat atactacccg tgcatttttg cccagcttag
    42241 cttttcttcg tctaaaatct caatataagt tccctcaata agaataatcc tatcccgtct
    42301 aaactccgag agtattctaa ccacgttttc gacccgcata tcaatgagct cggctatctc
    42361 ccgacgcgaa accggcaggt taaaccgtgt ggaatggtaa atttcgttgg caaaaaagca
    42421 aagtatgtag gctacacgcc ctctaagttg aagaagatcg atatcgagcc tgctgcctat
    42481 cagcaaatcg gaaaccctcg aaatcttggc aagtaaattc cgtgcaaact ccccgttgct
    42541 cccaactaaa ttcattacca tgtcgtactc cacgctgcag taactcgaat cctcaattgc
    42601 aacaatggag tagttgtacg ttttagaaga aaaaaggctg agcagcccaa caaaatcgtt
    42661 tggagtagca attcccacta tttgctccct cccttcagcg ttggtgcggc agagcttggc
    42721 cagcccatcc cttaaaaaaa taaaaccctc tatactgtcg ttctggcggg caattgtttc
    42781 accttttttg gcatgcatta gcactttgct cctcgacagc tcctccagct ggtaatcggg
    42841 aagcgaagca aacacctcgg aacgaaatcc gcacgtcagg cattcggtac ccggacacac
    42901 ttcagccttc atattcaaat atgttgatag ttatcaatat tgatttttaa caagatttaa
    42961 aactatacat tttatttaaa aagacttgaa atttgagctg aaatttataa ggaaaccttt
    43021 gcatttacag ctgctataaa aatgagccgg ttgaaacagt cataaatggt ttgttatacc
    43081 aatcagaagc attgtttgac tgctggaaga taagccatag cgttagaatt ggttaacaag
    43141 tacaaatagc tggccattta ggtgataagc cctcatgtag tacagcgtgc acaaacagga
    43201 atgaacaacc gggtattact tgcaaggttg gcaaagcaaa aaaatggttg ttcaattagc
    43261 tctacccccg atacccaaaa ggtatggttt aatgtatagg agatttgaaa actaaaaaaa
    43321 tatacgtatg aaaagaatcg taatacttgg tgcaggaaca ggtggcacta taatggccaa
    43381 caagcttcgt aaagcgttgg atagggaaca atgggaaatt accatagtgg ataaggatcc
    43441 ggtacattac tatcagccag gattgctctt catcccattt gggatatacg gcaagcagga
    43501 cgtagtaaaa ccaaaggcca gcttcatccc tgcaggagta aactttatta tcaacgaaat
    43561 tgaacgggtt gacccggcta aaaatagggt ttacctcgct aacggtcaaa cgctagatta
    43621 cgatttcatg atagtttcaa ccggcacctc catcagaccg gaagaaactc ccgggttgct
    43681 gggtaacgag tggggtaaga gcatttttga tttctacacc ttcaacgggg cagttgccct
    43741 tcaaaacttt ctgaagtcgt gggaaggagg caacctggtt cttaacatag ccgaaatgcc
    43801 ctataaatgc ccagtagcac cgctcgaatt tgttttcctg gccgatgagt ttttcgtaaa
    43861 aaaagggata agagacaagg tcaacatttt ttactctaca cccctttcgg gtgcatttac
    43921 caagccaaag gcaacaaaaa tgctgggcga cctccttcag gaaaaaaata tcaacctgat
    43981 tcccgacttt tacctagaaa gggtggatgc agaaaacaaa aaaattatct cctatgatgg
    44041 tcgggagctg cccttcgacc tgctaacgat tattcctgtg aataagggcg ctgatttcat
    44101 tgagcgaagc agtatgggcg atgacctcaa ctatatttca accgacaagt acaccctacg
    44161 ctccgagaaa tggagtaata tatttgttct gggcgatgcc agcaaccttc caacatcaaa
    44221 ggccggttcg gtagcccatt tctcctcgga cgtagttttc gacaacctca tttcgtcaat
    44281 cgaagggaga ccgctgatgg ccaaattcga cggccattca aactgttaca tcgagaccgg
    44341 gtttggcaag gggtcgctga ttgattttaa ctacgacacc gagcccctgc caggaaccta
    44401 tccggtgccg ggtattggac ccttcggcct cttgaaaaat acccgcatca accactacgg
    44461 aaaagtaatg ttccgctgga tgtactggca cttactctta aaagggaagg aattaccggt
    44521 tgattcgcaa atgtccctga taggaaagga actttaaggt tgcatatcat ggatgagaga
    44581 attattgagc agcagattga tgcccttaac cagaagctcg acaccgtgat cgagctgctg
    44641 gttagtcaac atcaaaaaaa cgaggtggtt gaagacctga ttgacgacct ctccattgtg
    44701 ggcaaaaacg cctttcaagc taccgtagat gagcttgccg ttcagaatat tagtattgaa
    44761 gcagacgatc tgaagtacct atttttcaag gttctgggca acatcaaaac attcggagag
    44821 ctgatggaca tgttggaaag tgtagtggac ctggttaaag acctaggccc aattgttcac
    44881 gatgccagca ttgcagtaac cgaaaaattg gggcaactcg accgggaggg ctacttttcc
    44941 tatcttaacc agctaggatt gctagcaatt gagtttaaga aaaattttac tgaaaaagat
    45001 attacacgcc tacgggagga aatgccagcc gttggagcca tagttagaaa cctttcacaa
    45061 ccccaggttg tcaacgcctt ggccactata accgaaactc tttcagaaat gaaactcgac
    45121 gataagcttg acaacaaatc gctcttcagg ctattcaggg agttaaataa acccgatgtt
    45181 aggaagacct tatcattcac ccttcggctt ttggctgaac tgggtaaaaa acaggatgtt
    45241 taactaatac cataagcgct atgtcagaaa aagtttatgc aggaaaatcg gtaaccgtta
    45301 ccgaagatgg atacctaacc gatccaaacc agtggacaaa ggatattgct gttgaaattg
    45361 ccagggagga gggcattacc ctaagcccta agcacttcga ggttattgaa tttattcgtg
    45421 ataaaacctt aaagggcgag gtgatgacca tccgcacaat aggaaaatcg gggataattg
    45481 acaccaaggg gttttacgaa ctcttccccg gtgcccccct gaaaaaggca agtcgcattg
    45541 ccggagtttc gaaaccaaca agttgcgttt aacctaataa caattaatta tggcaggaac
    45601 tatagatcac cctattaaaa aggtgctaat aatttgcgca aaagccaaca ttgaggatgt
    45661 ttacgctgcg ttggtaatgg ccaatggtgc tgttatggag ggcatcgaaa ccaaaatgtt
    45721 cttcaccttc tttggtcttg atgcaattac taaaaagagc atgaaaaagc tccacacttc
    45781 tgcggtggga aatcctggtc taaaaatgcc cggcggacta ccatttccaa cacttctggg
    45841 catactacca ggcgttgaag ctggtgtatc ggccatgatg aaaagccaaa tggaaaaact
    45901 tgatattcct actgtggagg aatttctgga gctgattacc gctggtggag gcgagatata
    45961 tgcctgcaag cttgccgtcg atatgtttgg tcttaaaaag gaagacttca gcgagcatgt
    46021 gaaggatatc attaccgtcg gacaacttta cgagcttgca gccggtgagg gatcacaaat
    46081 aatctttaca tagcatgacc tggtaaacac cgggcaacat aaaaacctgt catctacgaa
    46141 tagctgacag gtttttcaat tttacccgtt atccctcgcc catcaagagt tcaataaaaa
    46201 ttacctggtt tttcatacct ataggtttga gtttatgggg tattttctta cctttaaaca
    46261 aaaaaactac aagtgagcat ggaagaactt gttcgtttga ggcatgagct gcataggaat
    46321 cctgaacttt ccggacatga aagcgccacg gccaaacggc tgcgtttttt cctgagtagg
    46381 tataatcccg atgagctgta ctcgaacatt gcaggagagg gaatggccgt aatctaccag
    46441 ggaaaagagc cagggccaac tgtcctgttc cgctgcgata tggatgccct tccaattgcc
    46501 gagaccaatg acgtcaagta taaatcgaat gttcagggag tttcacacgt gtgtgggcac
    46561 gatgggcaca tgaccattat ttctggactt gccgttaggt ttagccaaag caaacctaaa
    46621 aaagggcggg tcattctttt ctaccagccc tctgaggaaa atggggaggg cgcccgaaag
    46681 tcgctggaag ggttaaaaga gctgggtcta aatcccgact atgctattgc ccttcataac
    46741 ctaccgggtt acaaaatggg aagcatcatc cttgggcggt acaccttctc agcggcatca
    46801 aaggggctgg tcatcaacct taaagggaag ccctcgcatg cggcctaccc ggagcatggg
    46861 ttgaaccctg ccgcggctac tgcggaaatc atccagtcgc ttctatccat tcaaagcacc
    46921 acccacttta aaaacttcat cctggtaacg gttatccaca ttcgggttgg cgacatagct
    46981 tttggaacat cccctggaaa tgcaaccata atggcgaccc tgcgagcctt tgacgacgac
    47041 gacatgaccc tgctgtcgaa aattgcagcc gaaaccgcaa cttccattgc ccataagcat
    47101 aagctgggta ttgacgtttc gtttaccgat gagtaccccg cctcggtgtg tgataaaacg
    47161 ctaacccaca cccttgaggc aattgcgcat gaagctagtc tcgaggtgaa tttcctcgag
    47221 caacccaaca ggtggagcga ggattttgca catttctcca cctgcttccc ttcggtgatt
    47281 tttggccttg gtgccggaga aaaccatcct gatatacaca cccccgagta cgatttcccc
    47341 gatgagctaa tagacaatgc ggttgacttc ttgggaaaaa tagcaaacaa gctaatggaa
    47401 ggttaagctt aaaccgatgc cggtgcaaag cttgcaagcc accatgttca aaccttttgg
    47461 aaaaagtagt gcatgcaata atttcattgc cattttcagt ttgattttgc ggttaaaaaa
    47521 ccattatagc ttccctgata acctgctacc ataactccat aaatgaaata agcacgttga
    47581 aagaaaatat aggtttttaa caaatcttct ttgccattaa aattatatct ttgcgccgtt
    47641 caataatttg aatatatgga cgattatcac cctgaccgtt aaacccgaat ggaggaacat
    47701 gtactgagac tccattcatt taaacatgga ggttagtaca tgaaatcatt ctggaaaaga
    47761 gatcttgggc ttgttcccag cgaagaatgg gagccctact gctgggtgga tgtcgaaatt
    47821 ccaaccgaag aggagcgaaa ataccttttc gacgagctgg gagttccgga agctttcttt
    47881 aacgatatcg aggacgtgga cgagcgtcca cgtatagaat ttgaggatga gtggtttttc
    47941 atcctgctac gaatgcccta taaaaatgta gatgtaagcc ttccttttac cactattccc
    48001 gttggtataa tttttaagga tgacattttt gtaagcattt ccttttaccg aacagaggtt
    48061 atccccgatt tcattcagtt caccatccgt aagggcattg tcataaaaga ccacttcgac
    48121 ctggtgctca ggcttatgct ctcatcaagc gtatggtttc tgaagtacct taaacaaata
    48181 aacctcgaca tcaggctcgc cgaagaccaa ctcgaaaaat ctattaagaa cgaggacttg
    48241 caaacgctgc tgagcattga gaagtgcctg gtttacttca ccacctcctt aaaggggaat
    48301 gatattttgc tacaccgcat taaaaattta aggaactata aggatacata taaccccgat
    48361 cttatagaag atgtagagat tgaactacgg caagctcagg aaacaacaag tgtgcattcg
    48421 gatattctta gcggtatgat ggatgcctac gcttcggtta tatcaaacaa cctgaacaac
    48481 gtgatgaggc agctaacctc catttcaatt attctcatga ttcctacatt gattgccagc
    48541 atctacggaa tgaatgtgcc aaacgggctg gaaaataatc cttggggctt tgggttggtg
    48601 gttcttgcat cattcctttt ctccattctg ggaattgtcc tctttcgcag gaaaaaatgg
    48661 ttttagcagc tgccagggtc aaaatcagct tataaaccaa acttgctgca agttgtagca
    48721 acagtcgttt tgggtaaata ccctgtagag tcgtaaaaaa aatcaaacct caccggctag
    48781 aggctgtgca tcagcagggg gtaaagttgc attgatacta aacctaaaat aaaaatcagg
    48841 aataagattt ccggccagta cttacccttt gcgtcggaaa aatactcgga aagcaacagg
    48901 ctaatgggag ctgctgcaat gtataatatt tcgtatgagc aggacggcac aacaaagaag
    48961 gaaagcaagg caacaacagt aaataaaagc ataatattct gatgcttcct gatggcaatt
    49021 ttctgacttc ccatgccggt taacatgtgt agaattgcaa ccagcaacgg aacgccaaca
    49081 gctacacaca gaataatcgg ggcaattttg tccgcagaac tttcggcttc aaggcaaagg
    49141 ctgtttgcca gctgtatgaa aggttcggat gcacctaaat caaaccagta gaagtagaaa
    49201 acaacagctc cccacggaac caaaatgccc acaagggtaa ccaaccattc acgcaggtta
    49261 aacttcctca gggtggcaat gctccagaaa atcaggaaaa caaatactac cgtaggggca
    49321 tacagtagag ttcctatacc tagcagtagc ccggagcgaa aaaggtggtc gagcgcttca
    49381 gaattctgat aggttgagat aacatggcta agtgaaacga gtataaaaat attagcaatg
    49441 atagcagggt ttaaacgttg aagcggtgtt aaggacgagg caaccaggac gaaaaaaagg
    49501 aaaggaaggt aggtgcgctg ccgaacaacc gtatgcctta cgttaaactg gagcaggtaa
    49561 atagcaacgg taagggtaag agcaaaggca accagtttag acagaaagct atacggcgat
    49621 aaaattttga caataagcac ataaagcggc attccaaagc tatcgaaagt aaaagtggaa
    49681 ggcgggtact tctgcataag cgttccggcc caaaaaagcg ttattactcc cagcatgaca
    49741 agcagagtag aaatcctaaa ccttctgaag aacttgagca gcatctggtt agcgtgttag
    49801 ctatgatttt gggtaaacat ccatacctat atcgcttcgg aagtacttgc tttcgaaggt
    49861 aattgcattg gcagcacagt atgatgaggc cagagcatct tccatgcttt ctcctgtacc
    49921 ggtacaggcc agcaccctac ctcctgcggt taacgtcatg gaaccggagc tagtggtacc
    49981 agcatgaaaa ataattgcgt tggcagaagc ctctaaacca ctaatagctt tccccttttc
    50041 gtagctaccc gggtatcctc ccgaggcaag gattaccgta gcggcaaatt ggggagctac
    50101 aaccagctcg gcctcgttca ttttcccggc agcacaaagc atgagcagct caagcatgtc
    50161 gcttttgata cgaggcaaaa caacctgggt ttccggatcg cccaagcggg cattgtactc
    50221 caccacgaac ggattcccca ccacattcat caacccgaaa aaaataaagc cacagtaggg
    50281 tattccctcg ctagctaacc cgctaatggt tggctttact atagtttcct ccaccttctt
    50341 catgaattcc ggagtggcaa aaggaactgg cgaaacagca cccattccgc cggtgttcgg
    50401 gccggtatca ccagctccaa cccttttgta atctttagct tcggggagaa taacatagga
    50461 cttgccgtcg gtaagaacaa aaaccgatag ctccaccccc ctaagatact cctcgataac
    50521 caccacgttc ccagcatcac caaattttcc gctgaaaaat tcgccaaggc tcgcacacgc
    50581 atcttcatat gaggcttgga taaccactcc cttaccggca gccaacccat cggctttcaa
    50641 aacataagga gaggaaagag ttcccagaaa ttttttggct tcgctgtatt catccctggt
    50701 gaatgctctg taccttgcag ttggaattcc atggcgctgc atgaacgcct tggcaaagtt
    50761 cttgctgccc tcaagcatgg caccttccct gccgggtcca accaccaaaa catttctaag
    50821 ccctaaatca cccgttagaa aattatacaa cccctttacc aatggctcct caggaccaat
    50881 aactaccagg ttaatgtttt tttcgagaac tactgtcctt atcccttcaa aatcgagcac
    50941 actaaggggt atattttcac ccaaccgggc agttccggga tttcctggta gagcaaatag
    51001 ttttcctaac tttgggcttt gtgaaagttt ccatgccagc gcatgctccc tccctccaga
    51061 gccaatgatt aacacgttta ctcccgacat tttatagatg tttgctacaa aggtagcaat
    51121 catttgggtt gacacaacaa cgtaaaccac atctgcattt cattgccaaa gccaatgtcc
    51181 agaatgtttt aactttttta caaccgttaa agtgctcaac aggaatcagc tcttgcaaac
    51241 tacccataat tgatgctaac tttaccgcta caattctaaa ataattccga atggttatct
    51301 ccatagttgt tgcaacttca gaaaacaggg taattggtaa aaacaaccag ctgctttggc
    51361 atatttctga agatttaaaa cgttttaaga agctaacaac cggacatacg gtgataatgg
    51421 gccgtaaaac atttgaaagt ataggccagc cactaccaaa ccggcataat atcgtaattt
    51481 cacgtagcga gggcttgaag tttaagggtt gcacagctgc aaaatcgttg gccgacgccc
    51541 tggagatagc ctcaacagat gaagaggttt ttattattgg cggcggggag gtttaccggc
    51601 aggcattgcc ccttgccgaa agaatatacc taacgttggt acatgctaac tatgagggtg
    51661 atacgttttt ccccgcatta aacccgaacg agtggaaagt tgtaagggaa gaaaaagtaa
    51721 gcccgaccga agggccgggt atctcctttt tagattatgt tcggcagcac tcctaagcag
    51781 ccttaagccg cttcaggttc gatttttcga gtttttgctg tgcgtaatcc aggttaacag
    51841 taatctcctt ttcactccct gttggtaaat cgaacatggc atccatcata atggcctcac
    51901 aaatagacct aagtcccctt gcccccagct taaactctac ggccttgttg acaatgtagt
    51961 ccagcacctc ctcttcgaac tgcaacttta tgccatcgta ctcaaaaagc ttgatgtact
    52021 gcttgatgat ggcatttttg ggttcggtga gaattttcct aagggcaacc ttgtccaacg
    52081 ggctaaggaa ggtgagcacc ggcaggcgcc caatgatttc gggtataaga ccataggctc
    52141 gtagatcctg cggagcaatg tactgtagca ggttgttttt atcaatgacc tcacgggttt
    52201 tccctgcacc gtatccaacc accgtggtat tgagcctttg ggcaattttt ttctcaattc
    52261 cctcgaaggc tcccccgcat atgaaaagaa tgtttttagt atcaacggct atcatcttct
    52321 gctcgggatg ctttctgcca ccttgtggcg gaacgttgac aacagagccc tcaagaagct
    52381 tgagcagtgc ctgctgaact ccctctcctg atacatcacg ggtaattgac ggattatcgc
    52441 ccttacgggc aattttatca atttcatcaa taaacacaat gcccttttcg gctgcctcaa
    52501 cgttgtagtc ggcaacctga agcaaacgag taagaatgct ttccacatcc tctcccacgt
    52561 agcctgcctc ggttagcact gtagcatcaa ctattgtgaa aggaacatgc agcatacggg
    52621 caatagtacg ggccaaaagt gttttgcctg ttcctgtttc gcctaccagc agaatattcg
    52681 atttttcaat ttcaacatca tcgcccatat ccttctgcag gagacgcttg tagtggttgt
    52741 aaaccgccac cgacaggtat ttcttggcct catcctgccc aataacgtac tggtcgagga
    52801 atttctttat ctcggtgggt ctcatcagct tgctcttctg cagcttgaac tcgccctttt
    52861 tcttaagttc ttcacttaca atgatgctgg cctgctcaac gcaaaggtcg catataaacc
    52921 catccatccc ggcaatcagc aagcttactt cctttcggct ccttccacaa aaagagcata
    52981 tatcggtgtt tttaaccatt attcctgaaa tttatttctg cgacaaaaat agcaaatttt
    53041 ttgcagcagg ttgctgttaa gctgggttaa tttggtttta ccgtgttgat tacatgattg
    53101 ctaccactac catgtttacc tatggtttct gatttagcat cccattaata acgaaatgga
    53161 gctcgcaaaa aaagcagatg tttaccgccc atcctctatg tattgcctcc actccagcat
    53221 gctcccccta aaaacgttaa aatcgacctt gccctttacc cccttaagcc tcccgttatg
    53281 ctcatactgc caaaatgtcc attccctatt gagctttggc ttatggttaa acgaacaaat
    53341 ccaaatatcg ttatctggaa aaacatcctg aatgtaaagc ttatagcaat cctcgttggt
    53401 gtaaataata attttttgat accggttctt ttcaacttta ctaacaaatt cgcgtagatc
    53461 ggcgatgatt tggcttcgcg acctgcggat gttgttgccc cactcctcca catccacaac
    53521 cagcggcagg ttatactttt gcctacctat ggtagaaaga aagttatttg cctgggtgag
    53581 gccatcccgg ttgaagttaa aaaaatgata agcacctaca ggaatgccgg ccgcccgggc
    53641 tccattaaca ttatcctgaa aatttttatc cttcagggtg gcaccctcgg aggccttcac
    53701 aaaagcaaag taaacacgtt gatcctttag cttttcccag ctgatgcttc cggtatgcgc
    53761 cgaaacatca atgccccgaa taacatacct atggtcaggt ttggaaataa atcgaataat
    53821 gcccagcatc agggcagcag agaggaaaac tgctacgggc acagaccaac ttttatgctt
    53881 cttcgatttt cttaccataa gaaattccac aaaggtaaag ccgttcaaaa tttcgcgcaa
    53941 caatagtagg aaaatcttta cattttatcc cgttaacgat agaagaaaaa ccaatgttgg
    54001 gaatgggacg aacagcgcta ttttgaagcc agaaacacaa atcgcctgta cagctataga
    54061 tacttatttc gtggagcttg gggtgtgtca ttttcaaccc atttcccatt acaacccggc
    54121 agctctcaac tttgtcaact ttttcgtgta actttgaatg ttttttgatt ttcgtaaaaa
    54181 tcctaatcca gcctatgcgg ttaaccagaa taactgccat actttttttc tcctgtatag
    54241 cccttgctgc caatggtcag gaaatcggca tgacaggaac aaaggcaaat gcggagcgtg
    54301 tcatggccga ttcgcttctg aaaaaccagc agtatgcgca ggcgctaccc atttactcaa
    54361 ggttgctgga gcagcactcc cgcgatcccc aaattcaact aaacctggga atttgttacc
    54421 tctacggaac ccgccgtatg gatttggcgg taaaattcct ttcgcaagcc tcaacgggtg
    54481 atgtaagcag catagtttac ttctacctgg ccgaagcact caggcaaagc taccggttta
    54541 gcgaggcaat tgattactac agaaggttta ccatcagcgg tggggcatcc gacattaagt
    54601 tggaggaggt tgaacagctg gttacccttt gcgagaatgg cagcttcctg acccgttaca
    54661 tctacaaccc tgaagtttac gacacaaaag ctgttgcagc tggcgaggcc tgcacgttct
    54721 actccatagc ccctaaatcg ggcagctttg ttaacgctcc aacaaactta ctcaccccta
    54781 cagacataaa ggaaaatcac caaccgctaa tgttctaccc cacaaaccca actcctggca
    54841 cttacgttta ctactccagc tacggcagca caactaccta cggtaaggat attttccgca
    54901 ttcagcttca ggataatgga tcctggagca aacccgaaaa tttgggtgac gtggttaact
    54961 caaactttga tgaggactac ccctacatca tacccgatgg cgttacacta tacttcgcct
    55021 ccaaggggca ttacggcatg ggtggatacg atattttcag aacggtattc gaccccacta
    55081 caaaacgctg gagccctccc gaaaatttag ctttcccgat taattcaccc tttgacgatt
    55141 ttctgtttgt ggccgatgat tcggatagcc tggcctgttt tgccaccaac cgcaaggtaa
    55201 tgcccgatac ggttgaagtc atactcatgc ggaatgagca aaaccctata cgccgctcgc
    55261 cccgctccta tagcgaactc gattcaattg ctgctcttaa agtttcgacc ttagcattga
    55321 atgccccaac aaaaaatcca accaccgtcc aggccgaaaa acctatagcc gcttcacaaa
    55381 aaccagccag cttcaattcg gtcgaaaacg atccggaata ctcccgaacc atagcaaagg
    55441 gttttgcggc ccagcagctg gctgactctc taaaaattag gctggagggg ctacgcggaa
    55501 aatttgattt cgttacaaca gccgaacaac gcattcggtt ggagaaacag gtggttcagg
    55561 tagaggatga catgctggct gcccaaaagg aggccgacct catgtttgta cgtgccagcc
    55621 aaattgaaca ggaatacctt actggaaaaa ggaaacctaa gggcgacgaa agcctatcgt
    55681 ttgtatcgga caaccccgac tacatctacc aggctcaatt tgcccctact gtctttcaaa
    55741 ccgatgagct gaaccgtttg gcacaagcag aacaactata cccgcaggta ttgcgaaacc
    55801 gaaatctagc cttaaccaac agggatatat acgaccaatg caaaaaaaac tcaaccgact
    55861 cctcgacaaa ggtttgtgat accaagtatg cggatatgct atcgtcaatg caaagctaca
    55921 acgagcagat ggggaaatac tatgaaaaaa aatatcccat ttacaacgac tgcgtacatg
    55981 tggccctggt gaaaagcgga tcaaacgacc aaagcattcg caagtgcatc agcactgcct
    56041 actcacactt tcgagcagcc ggcacaattc tgaacaacat gggtaacgac ggaagggttg
    56101 aaagcgcctt cgaggcaaac ttgcttagag aacttgggct cctccagctg gacctggctt
    56161 ttgccaaaat atggcacctc cagctctttg agcagaagtt gactagcagc attattaagt
    56221 ttgaacgatc ggcatttggc tctacctcag ctattgaact tttatcggca gagcagagca
    56281 agccacaaca ggcagctgcc tcgtcataca ccccgccaag gattgaaagg gtaagcagcc
    56341 ctgtgaacat cgaaaacctg gctgttgaaa gcgagatctc ctccgatttc ggtatagcca
    56401 aaaccagccc atacaacgaa gaaaatccta ttccggcagt atccgctcta cctaatggga
    56461 tttgctataa aattcaactg ggagtttacg gcactccacg ccaaccccag ttttttaagg
    56521 gtatataccc aatctcggcc gaacccgttg gcaagctaac ccgatactat gccggtgtat
    56581 tcgcaaggct tgccgatgcc gaaagtgctc tttcgaaggt gaaatcggtg ggctttaggg
    56641 atgcctttat tgttgcttgg tacaacggac aacaggttag cctccagcgc gcccagcagc
    56701 ttgaaggtag tctccaacag caaaagtcgt cagattccga ccgaaagggt gtttttgcga
    56761 tccagctggg ggtttacgag ggcagtatgc ccaacgatga gctaaaaacc atccgaacgc
    56821 taatccccaa caaggatatt acccgcaggg ttgatgaaag agggctgatg gtttactcca
    56881 tcgggaactt ctccaccctt agcgaagccg agcgtaacaa ggataacctg attgccggcg
    56941 gaattcttaa tgcaaaagtc atcgagttga atcaataaaa atttgctata ttagcaacct
    57001 ggactattct taaaaaacaa acacttttat aaccagaacc tctagttctt actgagatat
    57061 ggagttaaaa aagtggtgtt tttatgacat tcagaaaaga caagctgtag ccgggcatga
    57121 aggtaaaaga aaaaacattt ggtttggaac acacccagaa caagagttcc gaggtttaca
    57181 ccctattcct gcataacgac gatgtgaata catttgaatt cgttattcag gcgttggtag
    57241 aggtatgccg gcaggatgaa aaccaggccg agcaatgcgc tttaatcacc cattttaagg
    57301 gtaaatgcga tattaaacgt ggcagcttgc cagaacttga gcctatgatg gaggagctac
    57361 accggcgggg actaagcgct tccatcaatc taaactaaaa aattgcaatc ctcaatgatt
    57421 tggatattcc tactaattct cctgggtctc ttccttctgg ttgcagaagt gctgctagtt
    57481 cccggaatta ctgtagccgg aattgccgga ttcctgctga ttggctatgg agtatttgcc
    57541 gcctacacag agcatggaac catagctggt catataaccc ttgtattttc tatcgttgcc
    57601 tccgttatta ccatagtttt gtcgttacgt tcaaaaacat ggaaaaaaat cacccttaac
    57661 accagcatag attctaaagc caacatggat tacgagcagc acatccagcc cggcgacacg
    57721 ggagttacta taggaaggct tgccccaatg ggaaaagcca ccataaatgg acatgacgtt
    57781 gaggttaccg ccctcgatga gctagttgac ccacaaacaa cagttgtagt agttaaagtt
    57841 gaaggcagca aaataattgt taaacctaaa aaatagatta catgagccaa gttggagcaa
    57901 tacttaccat tatagccgtc tgcattgtcg gcttatggat tattttctac tttgtaccgg
    57961 taggtctgtg gtttcaggcg cttgtttccg gtgtcagaat ctcactcctg cagctaattt
    58021 tcatgcgctg gcggaaggtt cctccccggg taattgtgca ggccatgatt gagggaacta
    58081 aagccgggct taccctgaac cgtaatgaaa tggaagccca ctaccttgcc ggaggaaggg
    58141 ttccgaaggt ggttcatgca cttgtttcgg cccaaaaagc aaacatcccg ctcgacttca
    58201 aaatggcaac cgctattgac ctggctggcc gtgatgtttt tgaagccgtt cagatgtccg
    58261 taaaccccaa ggtgataaat accccaccgg ttaccgctgt agccaaggac ggcattcagc
    58321 ttattaccaa ggcaagagtt accgttaggg ctaacattaa gcagctggtt ggtggtgccg
    58381 gtgaagaaac tgttctggcc cgtgttggcg agggaatagt taccgccatt ggttcgtcgc
    58441 aaaaccacaa ggaagttctg gagaaccccg ataagatttc caaaacggtt ttgaacaaag
    58501 ggctggatgc cggaactgcc tttgaaatcc tttctattga tatcgccgac attgatattg
    58561 gacgcaatat tggcgctgta ctccagatgg accaggccaa cgccgataaa aatattgccc
    58621 aggccaaagc agaagagcgt agggctatgg ccgttgccct ggaacaggaa atgaaggcta
    58681 aagcacagga ggcccgcgct aaggttattg aagccgaggc tcaaattcca ttagccatgg
    58741 ccgatgcttt taaaagcgga aacctgggta ttatggatta ctaccggata aaaaatattg
    58801 aagccgatac caacatgcgc gacagcatcg caaaacccgg agttgaaccc aaaaagaagt
    58861 agatagcata acatcctaaa atagcttgtg caaaaaagag gggagttgcc cctctttttc
    58921 ttttcaaccc cacccgtaaa aaacctacca cctaccctgn nnnnnnnnnn nnnnnnnnnn
    58981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    59041 nnnnnnnnnn nnnnnnnnna aggtggcatt gaatctggct gttaaaaatg ctattcctta
    59101 cccaaattcc aaaaataacc atcctgatgt ctaaaaaaat atttaccaaa atatgctaaa
    59161 taccatacag gctattaagc aaaagctata cttttgctca acattttaat caatatcacc
    59221 aatttggtaa aaatttgata gaaaaaggca ttcatccggg gtagcaattc aagcattgct
    59281 ttacccgttg acttttcttt cggatttttt aacgagaatg gctacataac ttcataacat
    59341 attgttaaat agcaaggtaa ctatactaaa aaaatgaaga atattctggt tgtaggcgcc
    59401 ggcgggcaaa ttggctccga gctggttccc tatcttagaa aaatttacgg aaacgagcat
    59461 gttgtggcag ctgatatcaa ctcaagcgcc tgcactcacc tggccgagga aggtcctttt
    59521 gaggagctgg atgccctaaa ggccgacagg tatctcgaaa ttgtaaagaa ataccggata
    59581 gataccatat tcaacctggt tgcactactc tccgccaccg gcgaaaaaaa accccagctg
    59641 gcctggcatg ttaacatggg tgccctgatg aactccctcg agattgccag ggagaacagc
    59701 tgcgccgttt ttacccccag ctctatcggc gcatttggcc caacatcccc cctcgacaac
    59761 accccacagg ataccgtgat gcgccccaca acaatttacg gcatctgcaa ggtaaccggc
    59821 gagctgctga gcgattacta cttcctgaag tatggcgttg atacccgtag cgttcgtttt
    59881 cccggaataa tatctaatgt tacccttccc ggtggaggta ccaccgatta cgctgtggag
    59941 attttctacg gtgcagttca gcataagcac tacgtttgcc ctttgcccga gggaacctac
    60001 ctcgacatga tgtacatgcc cgatgctctt gatgcctgcg tgcagcttat ggaggccgac
    60061 ccctcgaagc tgaagcaccg aaacagcttc aacgtaaccg ccatgagctt tgaaccctca
    60121 caagtttact cggcaatcag aagacggatt cccgatttca ccatggagta tagcgttgac
    60181 ccggttaaga aggccattgc cgacagctgg cccaactaca tggacgactc atgcgcccgc
    60241 caggaatggg gctggagccc caagtggaac ctcgaaacca tgaccgatga catgcttagg
    60301 gcaataggcg aaaagatgaa gaagggactg cttgggtaat acccaacaac ggtagaacgc
    60361 acaaggtggt ttatttggat taaaccacct ttattttttt attccctata gcccttgcgc
    60421 ttaagggcat tgatgaagta ttccttatcg ggaaggagct tcagagccgg ttgttccaaa
    60481 accttgcaag ctctgcatcg aacctactcc ccttcggcga agtttgtaac ttcgtcgttt
    60541 ataaatcgcg gtttcccgac cgccgttaaa aaaattactc ttccccattc taaagtaaag
    60601 ctgcacacat actacaaccc ttaagccaag ctacaagtag aatcttgca
//
LOCUS       DAOSHM010000010        57645 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_412_length_57645_cov_20.426550, whole genome shotgun sequence.
ACCESSION   DAOSHM010000010 DAOSHM010000000
VERSION     DAOSHM010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 57645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 57645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57645
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_412_length_57645_cov_20.426550"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..531)
                     /gene="pncA"
                     /locus_tag="PK839_02810"
     CDS             complement(<1..531)
                     /gene="pncA"
                     /locus_tag="PK839_02810"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF008623.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HOG71698.1"
                     /translation="MKALIVVDVQNDFCPGGALAVAKGEIVIEPINKLSQLFESEGMA
                     VIFTRDWHPANHSSFKKYGGIWPVHCVAGTHGAELHADLYFPGVAILVSKATLPEKEA
                     YSGFQQTGLASWLHDMDVDHIVVTGLATDYCVKNTVFDAITLGFKVDVVIDAVMPVNL
                     NPDDGEKAIAEMVERGA"
     gene            complement(543..1760)
                     /locus_tag="PK839_02815"
     CDS             complement(543..1760)
                     /locus_tag="PK839_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG71699.1"
                     /translation="MKERLHSTTFDLPVQELRRGYRSDVYFWREKVVLESHNLHPHVT
                     MQVFQKNEAVVCGIDEAVAVLKVASGRYSDYDQAYKLFDKLIELKRMGRKVFLTDKKK
                     YLQLEEEKMKVSQALDKLWVDGFSDLKIDALWDGDAISPYETAMHIHGDASLFAHLET
                     IYLGILARRTRVATNVRRVVNAANGKTVLYFPARFDHWAVQGGDGYAAFVGGASGVST
                     DAQGEWWGAGASGTIPHALIASVGGSTVTSAKLFHETYPSADLIALVDFDNDCVNTSI
                     TCARELGDKLWGVRLDTSENLVDKSIFPEMGTFKPTGVNPKLVEKVRLALDKEGFKHV
                     KIVVSGGFTPERIGMFESMGVPVDAYGVGSYLMQGSYDYTADIVLVEDEPMAKVGRKY
                     SPNSRLQPFISTN"
     gene            complement(1821..2267)
                     /locus_tag="PK839_02820"
     CDS             complement(1821..2267)
                     /locus_tag="PK839_02820"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HOG71700.1"
                     /translation="MKSLGIVFIMILSPLVMLGQHVKMDKDGIKHGPIIGAIPDTVKL
                     GEIFLDELDDGNGKVQLTVVNQGNKPLILTRVSGCCGTNIKSWPKAPILPNQKGTIEV
                     YFRIENRPQRISRTVTIESNAANSPVVKVPIIGVVVERKAKNELTL"
     gene            2489..3157
                     /locus_tag="PK839_02825"
     CDS             2489..3157
                     /locus_tag="PK839_02825"
                     /inference="COORDINATES: protein motif:HMM:TIGR02009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOG71701.1"
                     /translation="MKTFSKAIDAAIFDMDGVLVNNHQYHLNAWKEFCRRHGYKFDEK
                     TFTTAYFGKNNQEIISELTGKSISTHEAELLGEEKEAIYRDIYLPFIKPVDGLIELLD
                     YLKENGVRLAVATSAPKSNFKFIEKSLRLENRFDVVVDASMVQHGKPDPEIYLKASEL
                     LKVHPEGCLVFEDSFSGIQAATAAGMRVVAMATTHTRNELGKNLTVADSFYDVLAFIL
                     RENE"
     gene            3359..5353
                     /locus_tag="PK839_02830"
     CDS             3359..5353
                     /locus_tag="PK839_02830"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HOG71702.1"
                     /translation="MKRCLVVSLIVLLAASAKGQNKEEDIFNLSLEELLNLEVSISTK
                     TATNIRNTPGILTVITSEDIKNSGVNGIIDLFQLFVPGFDFGVDVEGVVGMGVRGIWA
                     HEGKVLFMVNGLELNDGMFASVPFGNHFPLNNIERIEVIRGPGSAMYGGFAGMGVVNI
                     ITRDVYKPGGYVSYTANHTGRQFSQNNVGFGTGVHQNYLNIGISGNYGNGSRSDRDYV
                     DYHGQSRSMRNASDTYTRQLDLKLNYKNFNLVGFIDDYSYQQIDLWDSYYPGPPLTES
                     FKSSLVQATYDFKPNGNLTITPKISYKWQQPWKLTALGAGYGYNNNKTFDGLLGGIST
                     SYTKNKLTIVGGAELKHDHLNQPELVDTVYEETFRNGKEQLSYTNTAIYGLLQHDSRV
                     GNFTLGARYDFSSEYGSSFVPRVGIAKVFDFMHFKVMVSQSFRVPGGILPNRLPQGNP
                     EIKPEKGTSYEVAIGFKMPFQSYITFNIFDVSFDKVIVYNLGNDDITGVGYYKNEGTI
                     GTRGIEADFRHISTKISSFATFAYYMRKHSKTDSLYRVPDRESYFLGFSPLRLNGGFN
                     FRVNNRLSLTLSGSYFGKRYAFTQREGNGMDILEKLNPLFMLGCNVNIKNIVLKGLTA
                     TIEVNNMLGESFVFPQPYKGSHAPLPGLDRSFGFNLVYEY"
     gene            complement(5422..5889)
                     /locus_tag="PK839_02835"
     CDS             complement(5422..5889)
                     /locus_tag="PK839_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5606 domain-containing protein"
                     /protein_id="HOG71703.1"
                     /translation="METTLKEIISISGYSGLFKIVSQGRKGVIVESIADGNRMTAPIT
                     SKVTALTDISVFTNSTDLPLIEVLKLIRNLEDSKPTLNPKTSTDKAIREYFEKVLPDF
                     DRERVYTSDIKKILSWYNQLQANNLLAILDVEEAKPEEPAAEEHSNTAQDAES"
     gene            complement(6005..6295)
                     /locus_tag="PK839_02840"
     CDS             complement(6005..6295)
                     /locus_tag="PK839_02840"
                     /inference="COORDINATES: protein motif:HMM:NF016109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQ-loop repeat-containing protein"
                     /protein_id="HOG71704.1"
                     /translation="MNEAVEAIGWFGNIILSIGVIPQVVQTWRSHNVSSFNWVFLLLW
                     ALGVFFTFIYIAYNDMVSGKYQWPLWLNYLVNIAATFYLVWAKFHYGRKSNR"
     gene            complement(6305..7024)
                     /locus_tag="PK839_02845"
     CDS             complement(6305..7024)
                     /locus_tag="PK839_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HOG71705.1"
                     /translation="MGKYGKWISGGLGWALFGPIGAILGFAIGSIFDSAQTPTKIYTG
                     ETSRNGFLVSLLVLVSAVMKADGRVLKSELSYVKDYFYKNFGASASREAILLLRDILK
                     QSVPVKDVCKQVKENVDYHSRLQMLHFLFGIAQADGVVSPAELQIINEIAQYLGVETA
                     DFDSIKAMFIPDTDKYYKILEVSPSAANDEIKKAYRQMAIKYHPDKVSHLGEDVRQAA
                     EQKFKMVNEAYEKIKKERGFN"
     gene            complement(7029..7616)
                     /gene="coaE"
                     /locus_tag="PK839_02850"
     CDS             complement(7029..7616)
                     /gene="coaE"
                     /locus_tag="PK839_02850"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HOG71706.1"
                     /translation="MSLMVGVTGGIGSGKSLVCSILARLGYPVYNSDIASRTITDSDE
                     GVKQRVKLLFGEEIYCNGKLNRQRLASMVFADNMLLQQLNSIIHPVVMDDFEKWSETH
                     KDNQVAFMESAILFESGIFKKLDKTVLVVAPADVRIRRVMARDKVNKEAVQARVSNQM
                     DDELKVKLADFIINNDEKSLLLPQVIEIVERLKPL"
     gene            complement(7613..8611)
                     /locus_tag="PK839_02855"
     CDS             complement(7613..8611)
                     /locus_tag="PK839_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG71707.1"
                     /translation="MDSTLLKQFNKIIYREKANFKKRLAVFSLFLVISAILWFLLKLS
                     HEYSDELVYPVRYSNLQKGKIITGTPPEKISVKVKAYGYTLLRYKFTSYSYPLNIDLK
                     ELKPNQGKNSSSQFCVSTTQQFSSISAQLGKDMDVLDVFPDSICLELEDVVEKNVRVV
                     PNLSLTFARQYMLAGNIEVEPGEITISGPAAILDTINELKTQPVSLNLLAGNKKIILN
                     IPQVKQVFMPNSEVEVFIPVEKFTEVMLNVPIIPLNVPPSYDAILLPAEIQLRCNVTL
                     SKYFKLKPSNFRAVCNISDTGSMEGNKLKVLLENHPDFVSRVSFEPLYVDYILKKK"
     gene            complement(8681..8998)
                     /gene="yajC"
                     /locus_tag="PK839_02860"
     CDS             complement(8681..8998)
                     /gene="yajC"
                     /locus_tag="PK839_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HOG71708.1"
                     /translation="METLFVLLQKPAAQGANPLMTILMFALIIVVFYFFMIRPQMKRQ
                     KELRKFQEALKKGDKVIIAGGVYGKISEVKDDYVMVEISDNINVKVAKSTIIRDVSDI
                     QAK"
     gene            complement(9065..9484)
                     /locus_tag="PK839_02865"
     CDS             complement(9065..9484)
                     /locus_tag="PK839_02865"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HOG71709.1"
                     /translation="MRTRGMVVMCVLLLTTCGSPSKPKNNLSTSGNSSGVPNLEYIED
                     FFDFGTITQGEVVTHTFRFKNSGNAPLVVKDVIPGCGCTTSKLSSTILKPGEEGTVEV
                     IFNSQGWAGLQYKTITLRTNSSIMDKSVTIRANVVMP"
     gene            complement(9606..10601)
                     /gene="nusB"
                     /locus_tag="PK839_02870"
     CDS             complement(9606..10601)
                     /gene="nusB"
                     /locus_tag="PK839_02870"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOG71710.1"
                     /translation="MINRRLLRIKILQVLFAYFRSNNDSLSNLEKELHFSIEKSYQLY
                     LLVLLLPEALADVALEKIELGKQKFRPTADELNPNTRFVDNPLIHILRNNQALKQKAK
                     DSKLSWKNYPEVVKLLYQQLVSSESYHKFMSDDEFSLDTCRQMIVNFYLNELENSDEF
                     DAFLEEQSIYWNDDLKFVLSMAAKTIKKVREENPDALKLMPLYRNEEDKDFAFQLMTK
                     AVLKHSDNVGLIEEYTQNWEVDRIASMDMLIMEMAITEVLEFPSIPVKVSLNEYIEIA
                     KFYSTEQSGLFINGVLDKIITTLRQQKKFEKQGRGLIGDIAETPDDINSLEPDTE"
     gene            10825..11205
                     /locus_tag="PK839_02875"
     CDS             10825..11205
                     /locus_tag="PK839_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013547289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3276 family protein"
                     /protein_id="HOG71711.1"
                     /translation="MTDQLDNQNEFDRAEIEDLFSNVVKAGKRTYFFDVKATRSDEYY
                     LTITESKKRMGKDGNMFYEKHKIFLYKEDFDNFMGGLTEAVNFIKSNQEVLPRIAAEY
                     GNEASLAEVNDELTTLNFEDLDTK"
     gene            complement(11397..12167)
                     /locus_tag="PK839_02880"
     CDS             complement(11397..12167)
                     /locus_tag="PK839_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG71712.1"
                     /translation="MEANEPEEIFRQIQEFLGNAGKRLSIVEDVVDISIQKDYFRMAE
                     QFRNKQDTKEVNVSADILFSNDTTEEQLKEVLVLLASSGEVESYRAIEKFVSVAPSQL
                     KGWALLALNECRFHLVGALTDEEGVLISTGMGGLDGKLRYFIVLIPAVDMLSQQQAKL
                     VTDEVKFIFKHYDSEVERVEMADRYMCITALVPMSVPVNQPVTAVIKECNGMGNLLDE
                     GYIVTNVKTLAPDEIRKVLNREPIDGIYFDDNPDHTYK"
     gene            12377..14881
                     /gene="feoB"
                     /locus_tag="PK839_02885"
     CDS             12377..14881
                     /gene="feoB"
                     /locus_tag="PK839_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HOG71713.1"
                     /translation="MQLSELHNGEYGIISKIRGRGAFRKRITEMGFVKGKRVDVVKNA
                     PLNDPIEYSIMGYQVSLRRSEASLIEVVTPDEAKKILAENHTEPLTTLTEPELVQTGL
                     KVMGKTINIALVGNPNSGKTTIFNFLSGSREHVGNYSGVTVDSKIAHFQHKGYTFNIT
                     DLPGTYSLTAYTPEEVYVRNYIHENHPDLIINVVDASNLERNLYLTTQLIDMDVRIVV
                     ALNMYDELLDKRDRFDYEMLGKMLGIPFVPTVGSRAKGFDRLKDTIINVFSEKDKTSR
                     HIHINYGAVIEESIAAIQHEVRKNETLTVNYSSRYLSIKLLEKDRLTDRMLKDFPNYT
                     EIRKIARQEVERLEALFHEDSETLITDAKYGFIAGALKETYHESSIKKRQTTEIIDTF
                     ITHKLFGFPIFIFLIWLTFFTTFTLGEYPMNWIEAGVAWISRSINGLMPNGPLKDMLV
                     DGIISGVGSVIVFLPNILILFFFISLLEDTGYMARAAFIMDKLMHKIGLHGKSFIPLV
                     MGFGCNVPAIMASRTIENKNNRLVTMLITPFMSCSARLPVYILILGTFFPKHAGNLLV
                     LIYFTGILLAVGTALLLKKTLFRTDDVPFVMELPPYRIPTLRSILIHMWDKASQYLNK
                     IGGVILVGVIIIWALGYFPRNVQTSKDYDSLIGLKRAALTSAQATNAATAESLEQEIQ
                     EIEMEREALRQEQSYLGRIGKFIEPALKPLGFDWKMGVSLISGIAAKEIVVSTLGVLH
                     LAKEPNGSQSLPDKLKADTYTTGEKVGQKVFTPLSSFSFLIFILIYFPCVAVISAIKQ
                     ESGSWKWALFTIVFTTALAWVSSFLFFQVGTLITGS"
     gene            14883..15041
                     /locus_tag="PK839_02890"
     CDS             14883..15041
                     /locus_tag="PK839_02890"
                     /inference="COORDINATES: protein motif:HMM:NF024081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HOG71714.1"
                     /translation="MVQEIVVYMLIIAAILYVGYSIYRVFRPKQKNLGHCAGCSSSSC
                     GIRELKSK"
     gene            15114..15338
                     /locus_tag="PK839_02895"
     CDS             15114..15338
                     /locus_tag="PK839_02895"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HOG71715.1"
                     /translation="MKTEQFSVGKMKCMGCVATITSGLSEIEGVSKVNAELESATVTV
                     TYENDGLREVIINKLEALGYPVNLDIKQGS"
     gene            complement(15586..16371)
                     /locus_tag="PK839_02900"
     CDS             complement(15586..16371)
                     /locus_tag="PK839_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG71716.1"
                     /translation="MARTKHFGYFLFGGALTFLLMVTGSGRLDSATEVEPVDIRLFRC
                     YIYDDIKEWERVIGVLENTYRMKPTIDDEFQLLHAQYGFIPYLLGSNGNKEKAQRYID
                     LAEQHANNILKIKPGHVGAMAMNSALLAFKVYLSPYKAPFLGPKSIKIIDEALKLDAN
                     NPYPLLEKANFLQYAPGVVGGNPTEALGYYQKATEILRKGNNGQHPKTWWYMNAWAQM
                     ALSAEKAGKIDLARKTFKEILAISPDFKWVKTKLYPDFVKKHG"
     gene            complement(16404..17486)
                     /locus_tag="PK839_02905"
     CDS             complement(16404..17486)
                     /locus_tag="PK839_02905"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HOG71717.1"
                     /translation="MVIFYLVLLLVVFAVLRLVVVLVNYIYTPYLRPFELIDHGKVLV
                     VVGQGKNTASLVNLLHDLQNQSYTNFEVLVYCSSPLNSIPDDQLAFARVDNRFKFLNG
                     TIREAGESYSSNFRNVLVEKSSGSYILFLDPDMRMGIHFINAMVANMKHRKLAVLSVT
                     PHYDTTKSFGVKIAVSAVQWLMLSLIPLKLIGKHFNPKLPLTDGMPVMYNAEVFRRHT
                     LTSVTIPEDLKSAMYSKEFKQLRLRSFTLLGRKEEISCSYFTSYRYALRRVAGGLFDF
                     FSGREWLMFSYAILTTLGPFITLLAMPYPFTFAYLFSVLLGRMLVAALCEQSAWSAIL
                     LFPLQQLVLFRMMLSPLHSSITGHKR"
     gene            complement(17810..18322)
                     /locus_tag="PK839_02910"
     CDS             complement(17810..18322)
                     /locus_tag="PK839_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005782541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HOG71718.1"
                     /translation="MGKEAKQVVSVDLEKLLGMLNAALAEEWLAYYQYWIGARVMEGP
                     MRSEIEPELLLHATQELNHAVLVVGRIIQLGGTPILSPQDWFKVNRCTYDAPVDPYIE
                     VVLEQNLKGERCAIKRYQEIADFTNGKDHSTHQMAVQILNEEIEHENDIEDWITDINR
                     MKEEFKKMRM"
     gene            complement(18423..19103)
                     /locus_tag="PK839_02915"
     CDS             complement(18423..19103)
                     /locus_tag="PK839_02915"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOG71719.1"
                     /translation="MSYPECRTCGYKSSAAKHLNETELQTLEFGCAEVLFKKGEVVFR
                     EGTLATNTIYLKRGMVKVHMKGPSGDKILKLIKAPTYLGLPTTFGDRINQYSATAISD
                     AEVCFISLDTFKDFITKNGDFAYELIMDLCRNELVEYRRCTSMSQKQIPGRVAEVLLC
                     MSDDLFCSSTFEMPLNNSELADLISTSRETVNRQLSEFIREGLISMTKRKIQILDAKR
                     LGQISING"
     gene            19276..19821
                     /locus_tag="PK839_02920"
     CDS             19276..19821
                     /locus_tag="PK839_02920"
                     /inference="COORDINATES: protein motif:HMM:NF025066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HOG71720.1"
                     /translation="MEIDVEKQLEELKKKVGKLESGRKDKLTMVVFSGDMDKLLAAMI
                     IATGATAMDMEVSLFFTFWATAALRDPKKKVKGKNLVSKMFGFMLPKGAKKVKLSKLH
                     MAGMGKTMLENIMKKKNVASLDEMFKTAAELGVSINVCEMSMNLMGFKREELIDYPNL
                     KICGVGTFLSDAEESKIQLFI"
     gene            19845..20207
                     /locus_tag="PK839_02925"
     CDS             19845..20207
                     /locus_tag="PK839_02925"
                     /inference="COORDINATES: protein motif:HMM:NF014672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HOG71721.1"
                     /translation="MSEKLIVIVTEGPDNQEKATIPFVMATAAQSMDVDVTIILQAEG
                     VLLVKKGVSENVNASGFMPLKQLLDTFIELNGKLLVCTPCAKSRGISENDLVEGSKLI
                     AAGTVISESLSANAVLSY"
     gene            20230..20481
                     /locus_tag="PK839_02930"
     CDS             20230..20481
                     /locus_tag="PK839_02930"
                     /inference="COORDINATES: protein motif:HMM:NF013379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HOG71722.1"
                     /translation="MNAEELKNLKVSQTVDARGTACPGPLLAAKKAIGEIESGEIMEV
                     LSSDEGTKHDIPKWCEKMEHEFLGIIEEDSFTRLFLKKA"
     gene            20570..20998
                     /locus_tag="PK839_02935"
     CDS             20570..20998
                     /locus_tag="PK839_02935"
                     /inference="COORDINATES: protein motif:HMM:NF014692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HOG71723.1"
                     /translation="MLSETFSPKILVFSTDIISDPGIDQAGLRKLHYPPTVYVISLPC
                     SSGIKPRWIMRAFEKGFDGVFIAADGHECSYSPKCAEHTNNIIVESQKLMKENNINPK
                     RIRMAALCSVCAEPFVNHMGNFSKILSELGSIGTEKYNPS"
     gene            21123..22454
                     /locus_tag="PK839_02940"
     CDS             21123..22454
                     /locus_tag="PK839_02940"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit A family protein"
                     /protein_id="HOG71724.1"
                     /translation="MPENKKTFDALVVGGGIAGQEAALSLANMDYKVLLVEKELSIGG
                     KMIQLSKVFPTLDCAACITTPKMSETARHPNIQLLLNTDIMEITKNNGLFNVNLTIQP
                     TYVNPDLCTGCQLCEWACPVVIVDKYNNNMVGQKAISIAFSLANPKVAAIDMNHCIHC
                     KKCEKVCEPKAIDYEMQPKNINIETKTVIIATGFNLFKGENKPQYGFKKFPNVIDAMQ
                     MDRLIAPTRPYNAVLRPSDGKVPDKIAYVLCTGSRDSSLENAIGCGAECSNNPICSQI
                     CCMYSIKQAQLLMGALPLADITIYYMDIRAFGKGYEEFFQQSKGMGVNFIKGKVAKIN
                     QKDDNSGDLILRYEDVTTGALKEANHDLVVLSVGVLPNNGITKVFTDDKLELDNQGFI
                     KSIDELVSPSLTSIDGVFVAGTAAGPKDIPDSILSAGTAASEAASYINSTL"
     gene            22532..24505
                     /locus_tag="PK839_02945"
     CDS             22532..24505
                     /locus_tag="PK839_02945"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit A family protein"
                     /protein_id="HOG71725.1"
                     /translation="MMKQRIGVYICHCGGNISDYVDVEQLGKLMAKEDGVIISKDVVF
                     ACSDSTQKDIEKDIKEMNLDAIVVASCSPKLHTHTFRGVAERAGINPYNYVQVSVREQ
                     CSWAHSDKPMDATIKAYGLIRAGINRVSHSEALEIIEIKAQKAVAVIGAGVSGLRAAI
                     DLARMGNQVYLIEKEAEVGGKVLTSGKLFMTGENGKEVIDRLYQRIKEMNNINIFTNA
                     NLENISGSFGNFMIDVNVAGELLKLNVGSVLVTTGFDFYQPKEGEYGYQLSDKVITLP
                     QFNKLINSNSGKLTYNGKTIKTIAYIYCVGNRQSKGENKYCSRQCCTAAIYSSLNVHE
                     KFEGIKAFHLYRDIRTYGKQELLYQESSRKGDVYIKFEEKEPPVIEVNEDKLSIKVKD
                     YLTSKKELMLDADLVVLVTGMVSRSDSSDISGKLKIPIGNDKFFNEIHPKLKPVETVI
                     KGIYIGGACQGPKNISESVQSALSGAAKINAAICKGSIELEPIVARINSETCIWCNKC
                     SEVCEFDAIKPIESNGKMIAEVNISSCTGCGICAPVCPTNAIEVAHFTDNEIESMIDG
                     FASPVNILPREHANSKTSSSKAQSLAGYPKIWRTIANALTEKPLTIPQIAEQINISVQ
                     DVTWNIMTMNRYGFIEPAGLDDDEAYYMYKLKK"
     gene            24511..25587
                     /locus_tag="PK839_02950"
     CDS             24511..25587
                     /locus_tag="PK839_02950"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOG71726.1"
                     /translation="MPHINPDFADEIKKYGADDFNLCFNCGNCTAVCSLSTNDESFPR
                     EMIRYSTLGLEDEIKSSLKPWLCYYCGECSTDCPRQANPGELMMSLRRWLTAQYDWTG
                     LSGLLYKHIGAYIGGMLVLLAGVIGFTNFSGFDREQVIHYGHLFEMLAIAGVFAAILL
                     PNIIHMWYLTIVKPKVKPSLKVYAQSTGDLFVHMFTQKRFLGCEDTRENRTRWILHFI
                     LVLSYLSLLFTTVFLNWFSTTSKFVIWLGYFESAAIFIITTVFMSMRFRKVDTMHKHS
                     QPSDWFFVIWLFLMGITSFAVRLLIDLNNLDSQFWMYTLHLTVLAQWALIIVPFGKWT
                     HFLYRSFAMYFQKILELNRTVKRP"
     gene            25676..25978
                     /locus_tag="PK839_02955"
     CDS             25676..25978
                     /locus_tag="PK839_02955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG71727.1"
                     /translation="MERKQRKYQEKAHQTLDEIFESIDSLDEKIRQASDKASSTMKEN
                     IEELKAEREKLKEKLKELNEASGESWKEIRNGFEEAATSLRESFIRAKNKFQDSEQ"
     gene            26054..27250
                     /locus_tag="PK839_02960"
     CDS             26054..27250
                     /locus_tag="PK839_02960"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HOG71728.1"
                     /translation="MMELRKWLLVFSTVFSAIVFAKSQEADSSSLTLLFVGDVMGHSP
                     QISSALNPLTGEYEYDSVFTRMKGVFSMADFTIANLEVTLGGEPYTGYPQFSSPDALV
                     DGLINAGVNVLVTANNHSADRGAKGIQRTVETLDRKGIIHTGTFSDSSDRLKNNPLIL
                     EKNGMRIALLNYTYGTNGLPVPAPCIVNKIDTLVIIRDIAMTRLMNADEIVVFIHWGN
                     EYERYPSAAQVAMANFLNRQGVRIVIGSHPHVVQPMEATLDTDSTTGRIVVYSLGNFV
                     SNQRRRYCDGGALAAVKLVKKSTGKSEITAAGFLPVWVHTPSTASHKTFQVLPVSIYE
                     TAPKGYFSADELAQLTQFATDTRALMHEKNLNFPELIFYNGSWIIPDNRDKLPITRLT
                     TGQSHF"
     gene            27475..29307
                     /locus_tag="PK839_02965"
     CDS             27475..29307
                     /locus_tag="PK839_02965"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOG71729.1"
                     /translation="MKQRFSFLIRFFLFWLIYFIVFRAVFLVYQLPLTRGISMGDALM
                     IFTRGIWMDTSLAGYILLLASLFMALLYFVHGRFIRTVYTTYTIILLVLFSVITISDL
                     ELYRNWGFRIDATPLLYLKTPKDAMASLKGWVILVLAIAAAIAVISSMFFYRKWVAKP
                     LNHIEKGKWWYFPIFTFIAACMIIPIRGGFGIAPMNPGKVYFSQNVYCNHAALNPVWN
                     MMYSVSKSGKMSKQYPTEIKPSEAKQLFTSMMESDSTNYVLSSNRPNIVVILLESFSA
                     KLIEPLGGKSGVTPCFNRLSEQGILFTDAFASGDRSDKGIIAVLSGFPAQPTQSIIKY
                     PTKSRKLPTISGVLAENGYNNTFYYGGDPDFANIRSFLYHAKFNRIVTQDEFPKKYRN
                     SKWGVHDEYVFNYLLTDLDSARAPFFKFFFTLSSHEPFEIPAKPKFKGSDEVNQYLSS
                     AYYTDSCLGNFFNIAQKKNWYKNTIFILIADHGHRHLGNHPNYAVEKFHIPMLWIGGA
                     LSVNPTRIDETVSQVDLASTLLNQLNLSDSAFTFSKNILSSHFTPFAYYAFNDGFGFK
                     NNTDTVIYDHAGKKYIKMSRANPKQAAQEAWAFFSTYQDTFLGY"
     gene            complement(29363..30448)
                     /locus_tag="PK839_02970"
     CDS             complement(29363..30448)
                     /locus_tag="PK839_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG71730.1"
                     /translation="MKAFLRISVTLFLLLSSLSGYSASLELSKQYDREFKVSPASKLY
                     VENKYGQISIENWDKSAIAIHVDVKVENANAERAKSMLDAINVEFSETENLVSAITRF
                     NEDIRKSSPRLFSSFSNTELSINYKIFMPKNIEVELLNKYGNIFVNELNARITVDLMY
                     GNFKANKLGLGDSQPLSSITVGYGDVAISEANWLKVEMKYSNLNIEVAQAMVVLSKYS
                     KVYLGRVNSLVLESKYDRYEVGSVMNLEGESGYTDFDISNLGKKFNLTTRYGNVKIID
                     VSDSFQSIRFEGAYTGLKAGINPNVSYRIDAEAAYGSINFSADKSSVNRIEDNTTIQV
                     SGLVGTNSRTNAEVYIRIKYGNANLNK"
     gene            complement(30496..31011)
                     /locus_tag="PK839_02975"
     CDS             complement(30496..31011)
                     /locus_tag="PK839_02975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG71731.1"
                     /translation="MDKLEKFFAENKGQLDDVDLPDGHEQRFKSRIHAQKNHTTIKTF
                     ALAAASVSIILALTAALSLMYGNHFSEELSRHFSKGSTAQFMEVEAYYRSQLLQKYRA
                     IEKLASSTDPSVKPEAKQLLEEFDREGQRLKAELPKNPRSDMVLGAMYENYQSRLEVL
                     QKIEGTLDKGK"
     gene            complement(31023..31550)
                     /locus_tag="PK839_02980"
     CDS             complement(31023..31550)
                     /locus_tag="PK839_02980"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOG71732.1"
                     /translation="MDISSELVKKCCRGDSRAQFELYSMYSRAMYNVSCRIIPDRMEA
                     EDAMQEAFFKAFDRISTFRGEVTFGAWLKRIVINTCLDHLKKRRVQLVSFDDLPGLAE
                     HTDSSDDYTPESVEQVKLAMQQLPEGYRVVLTLRLVEELDYPEIAEMLDINESSVRSQ
                     YTRGRQKLVELLKRN"
     gene            complement(31763..32926)
                     /locus_tag="PK839_02985"
     CDS             complement(31763..32926)
                     /locus_tag="PK839_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HOG71733.1"
                     /translation="MEQSGGFFSFLVENLNQFLSFTGFANFTIGHGVMILVGLFFIYL
                     AISKEFEPMLLVPIGFGIIVGNIPFSPGYQIGVYESGSVLNYLYFGVLNGIYPPLIFL
                     GIGAMTDFSALISNPKLMLIGAAAQLGIFGAYILALVLGFSPQEAGAIGIIGGADGPT
                     AIFLSSKLAPELLGAIAISAYSYMALVPVIQPPIMRLLTTRKERVIRMKPPRQVTKLE
                     KILFPIVGILLTAFIVPSGLPLLGMLFFGNLLKESTVTKRLAETARGPLIDIVTILIG
                     LTVGASTQATTFLTAKSLEIFIIGAISFIVATFGGVMFVKIINLFLKEGQKINPLIGN
                     AGVSAVPDSARVSQVIGLEYDKTNHLLMHAMGPNVAGVIGSAVAAGILLSFLA"
     gene            complement(32929..33366)
                     /locus_tag="PK839_02990"
     CDS             complement(32929..33366)
                     /locus_tag="PK839_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018965432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HOG71734.1"
                     /translation="MKDFKFKINGNEYSVHIANVDGNLADVEVNGTPYRVEMERELRQ
                     TKTPKLIRQEAVPATDSHPSVVKTVSPDSPSTGMVKSPLPGVVLDVLVKPGDEVKVGQ
                     RLIVLEAMKMENNIDSDKAGRVTEVKVRKGDTVLEGEVLIIIG"
     gene            complement(33408..34331)
                     /locus_tag="PK839_02995"
     CDS             complement(33408..34331)
                     /locus_tag="PK839_02995"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008948 - oxaloacetate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family transporter subunit"
                     /protein_id="HOG71735.1"
                     /translation="MMKKFIHGILAALLWMGAAVSSTAQSVYDLRINEVMLFNTENYI
                     DSYGQRSSWIEIMNLGYNTVNIGGCYLTDDPTQPKKYLIPKGDPVTRIPQQQFLVFFA
                     NGKSHHGVLHLNFTLDSTHRFVALYSSDGRSLIDSVTVPLSLPNTSYCRIPDGTSAWQ
                     ITSFTTPNATNNLFTHEETSGEKFVQFDPFGIMMTCIAMLVVFTALFILYRIFRFLGM
                     MMQKPLRVGKRESRDVGKTQEVQVAELSGEILAAVSAALYLYETEQHDQESEIITIQR
                     VSRHYSPWSSKLYSLTQQPNRVVTHRVHNKQ"
     gene            complement(34354..35916)
                     /locus_tag="PK839_03000"
     CDS             complement(34354..35916)
                     /locus_tag="PK839_03000"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HOG71736.1"
                     /translation="MSIQEQKIKELIELREKAKLGGGARRIESQHKKGKYTARERIEM
                     LLDEGSFEEFDMFVTHRCTDFGLDKETYLSDGVITGYGTIDGRLVYVFSQDFTVFGGS
                     LSEMFAAKICKVMDMAMKVGAPVIGINDSGGARIQEGVTSLGGYAEIFERNILASGVV
                     PQISAIFGPCAGGAVYSPALTDFIVMSRNTSYMFVTGPKVVKTVTGETVTSEELGGAG
                     VHSSKSGVAHFVAENEEEGIMLIRKLLSYLPQNNLEEAPIMPCDDPIDRLEDSLNDII
                     PDNPSKPYDVKDVITAIVDYGEFLEVQRDYAHNIITCFARFNGQSVGIVANQPKYLAG
                     VLDINSSRKAARFVRFCDSFNIPLVTLVDVPGFLPGTGQEYNGIIIHGAKLLYAYGEA
                     TVPKITIILRKAYGGAYDVMSSKHLRGDINYSWPTAEIAVMGPKGAIEILYSKELETL
                     EDQKAKDDFLLRKEEEYKNKFANPYVAAKYGYIDDVIEPRNTRFRVIRALQSLATKKV
                     VNPPKKHSNLPL"
     gene            complement(35938..36351)
                     /gene="mce"
                     /locus_tag="PK839_03005"
     CDS             complement(35938..36351)
                     /gene="mce"
                     /locus_tag="PK839_03005"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009433153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HOG71737.1"
                     /translation="MSVTHIEHIGIAVKSLNDAIPYYENLLGVKCYSIEEVKDQKVKT
                     AFFKVGQTKIELLETTDPEGTIGKFIEKKGEGIHHIAFAVKGINEELQRMQENGIVLI
                     DKTPRSGAEGLEIAFLHPKSTFGVLTEFCEDRNQK"
     gene            36786..37532
                     /gene="mtgA"
                     /locus_tag="PK839_03010"
     CDS             36786..37532
                     /gene="mtgA"
                     /locus_tag="PK839_03010"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007560507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HOG71738.1"
                     /translation="MNGKNRQKSQRYQSFRRLVLYAIATFFITTVISVIAFSFINPNL
                     TMLMVAKRVQPLNDGGSNRINHKWVSIDKISPQLVLAVCAAEDNKFMEHNGFDWESIQ
                     KAKEHNKKGKRLRGASTITQQTVKNVFLWPRRSWVRKGFEAYFTFLVEVFWSKERIME
                     VYLNVAELGRGIYGVESASECYYGKPASKISTYEAAMLATVLPNPAKRNPAKPSSYMY
                     RYQRTILRNMYNLGRIDLKGDTSSKSKKKN"
     gene            37537..39504
                     /locus_tag="PK839_03015"
     CDS             37537..39504
                     /locus_tag="PK839_03015"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG71739.1"
                     /translation="MSKLKRHLPILFLIILGSLGHSPLYGQLNKAYYYYKSEELISQG
                     RYSESLPVLNTLIGIDSSLFEGWFLRGVAKYNLGDIRGAISDFDKSLKANPLSAQSYH
                     YKAIALSQVSEYDKALKEISSALELRPNSPDFLFTLGVLQFQTQNYPKAIGTFTRVIQ
                     LESSNADAWYNRATAKLLIADTLGAISDYSTTINVNPFHAAAYARRGAVNAELDRNHL
                     ALADLNRAIELDSLKADFYFTRAIINYNFKKYQNSIDDLTKALRLNPNYQIALFNRAI
                     INYQAGNTDPAIDDLTRLMAINSQNVLVPYYRGSIYLEKGEATRSIDDFTKAISLFPD
                     FANAYLLRSDAKRQLGNLPGAEEDFLKGQALANKFHGKGQAEISSLLDSSGKLNKLIS
                     LESDFNLASTLSIESNRIRIASAFMPMIRLHFNQPDKPATNNNLASVYLDSINATLPI
                     NYKLIFGFAPSNSTEKLSIESADSTIANFPALCLVKGIFLAENGKFASATNEFKKAFE
                     QNSSNPIYLFSYIMNNVEMAEFIENFNTSTEISGIAMSSSKNTGITSITAYNDAIVEL
                     KGLYEVFPKNGLIAYNLGNLSIMTNDYESAISWFSLAVRENPLLSCAWFNKGLSLLIT
                     GNKDEGCNNLGKAGELGVEEAYMAIKKFCTR"
     gene            39738..40457
                     /locus_tag="PK839_03020"
     CDS             39738..40457
                     /locus_tag="PK839_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOG71740.1"
                     /translation="MGKLFDRLNSDVRFIEGINGCINCGTCTAICPAAEVYDYEPRSI
                     ANLVQTHDDEVIENLLKSDTIWYCGECMSCKTRCPRNNAPGLIIQALRGLSIETGHFV
                     ESEKGRQQLSLKRMLGNSILETGYCVHFDHTELSMFPELGPVWQWVRDNRETVLKKVG
                     ANYKGTGPGAIRKISQESLDELERIFEVTGGLEWFKKIEEDSSEMADKLSLEIGNETS
                     KESPYFRKVYRHNDPEKHTRK"
     gene            40570..41646
                     /locus_tag="PK839_03025"
     CDS             40570..41646
                     /locus_tag="PK839_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HOG71741.1"
                     /translation="MKGNEVKKKIWSEYQKEIADDKFYFVRSCIRQTFFPGSDTVYLK
                     ILRDVLGKDVYEDANHTTCTGIGYHCEVVPFPTIQTVVARNFSLMRENGYQNYNPSCV
                     TSFGIYTEILETWHHFPEEKEKTREYLKKATGREFEEPANLVHTSDVLYKFRNEIAAK
                     AKYRLVDRETGKPLKVVDHIGCHYAKMFPSKGIGGAEYPQVLAGLVEAFGGEVVDYPE
                     RRHCCGFGFRQYFVQANRGYSIAASKKKFESMEPFKPDFIITNCPGCNYFMDRWQYVL
                     AEAEGKTYGQDGYGIPVLSYEEVAGVVLGFDPWEIGLQMHQTDVEPLLKKIGVEYDPD
                     KKFKGVNNETLSLPDKPNNIKFIV"
     gene            41795..42817
                     /locus_tag="PK839_03030"
     CDS             41795..42817
                     /locus_tag="PK839_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOG71742.1"
                     /translation="MLKKVAVIGGGVAGLEAARQLAALGYQTTVYEKNDHAGGHTSEW
                     DRLFPNRRPAVEVVDTLKSQLNGNPAIVLSTPITELLPNGDKFTLITSNKSFEADAVL
                     IATGFELFQAERKEEYGYGIYDNVITSAELEKMFTEHGAPLMRNGEKPKRVAFVHCVG
                     SRDEKVNHLYCSKVCCVTAVKQAIEAKEAIPEAEIFCLYMDLRMFGPGYEELYKEAQQ
                     KGINFIRGRLSEASELDGGRLLIKTEDTLASRPLKMSVDMLVLMVGMTPAEGTSKLIY
                     NLGLKPNADGFIGVSDYHLKPSETGIRGVYTAGACSGPNNITDSINSARSAVVEIHRY
                     LSVKEP"
     gene            42814..43149
                     /locus_tag="PK839_03035"
     CDS             42814..43149
                     /locus_tag="PK839_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOG71743.1"
                     /translation="MIDFGYAPLKINIHDLDKADLSLMDWITARDESFRLCIACGSCT
                     ATCTAGNFTNFSFRELCHSIRRGEVKKAVRESEKCMLCGKCTLVCPRNVNTRNIIKLV
                     RKANQNFRV"
     gene            43146..44999
                     /locus_tag="PK839_03040"
     CDS             43146..44999
                     /locus_tag="PK839_03040"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOG71744.1"
                     /translation="MKEHPFVIPFTIGLTFVILYTVVMWTIWVVRLTRREKKIIWKRL
                     LTVKTLRGIWETIREGLFHRNIFKKNVILGFMHMSLAFGWFMLIVLGNLEARLYPHGL
                     INPPYYPIFFEFFRHDLSPLREYEEIFTFLMDFFLLLVISGIGIAFFKRYKSKVMGMH
                     RTTKHILIDRIALTSLWLIFPFRILAESVTAGLYNTGHFFTNFLGQQVIYGLPLDQLY
                     YPSWWAYSIALGSFFVALPFSRYMHIPTEILLIFLRKYGVREKDSHSSYTNVELNACS
                     RCGICIDSCQLSTDLGISDTQPTYFIRDLRYSKPSERLRETCMLCMRCLKACPVGIEI
                     TQIRKNERSEHQLFSPTSYSYLNGYAYPKAKVAFFAGCMGHLTPNIIKSTLLLLDAAG
                     IDYTFIDRNGSICCGRPLQLNGEVDAAKQLIDKNNRLIYETGANCLVTTCPICASEFK
                     KNYHMSIPIYHHSQYLWELTNQGRLVFNQSNLNVAYHDPCELSRGLGVVDEPRKLLKS
                     AVVLTENSNQTKQNSLCCGGSLAITNTSAFQKSTIAASTVLQLANNTTDLIATACPLC
                     KKTLAAAQNDVPVQDISEIMVKALMDSRKASLSEAKVKMASPKLEVVEEYK"
     gene            45286..48033
                     /locus_tag="PK839_03045"
     CDS             45286..48033
                     /locus_tag="PK839_03045"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOG71745.1"
                     /translation="MKKFLLASVVTLGSSWALLAQPERMPFGENMQYSSGKHLFNAES
                     SGAKGINEMYFSDDFEGSSYILSDKWEILRSSTLDYSSTISAANPMWFHCTPESFNGN
                     GATYIKFGRGSAAISYTAPDFTWLVCKDTLPIPQNGEAYLKFWLWYSSNFDQGYSTHF
                     YILAYNTDDHAIDTLNYWGEYDSTSISNQYARRITVPLSLLAGKNIRLAFVYENRNNE
                     NKGIQLAVDEVEITNSNSPDISLKAVPFTYSQIPYFTFDSLFFNLYADIYNTGAAISD
                     TIEIVATSEQLPDINAFATITDTLNEGEQRLAKLNGQAKITENTNYTIGITSSTDQDS
                     ITANNSDNIVFSVSQSVYATDHGATGGFTLGENQPFGNIFKVNKPSFIKGIQVGWSPI
                     STIPEAEYPIPFWVAVYQLDPLDSSCYFPVKTGYFEKQYSQVGASSDYPFDEPIYCLP
                     GFSYFVTVTQLSSASLGVGCDGNSYGSFWKRNGSTFKPEKLANPTIGNAAIRLYFNKP
                     IENPIVTFTVKNENNEPVQNARVYVREVDSTLITGVSGEASIELSNGTYTCRLDSAGF
                     APVTKQVKVFYQDLFIDFKLQKTYQAKFKVLDEDLLTPIAGAEINILSESIDITFPEE
                     PYLTDQNGLDSLQIAPGYYNFSVRASGYNMSSPGYFFIEPSDTLIEVFMQPATTHSLT
                     LQVIDEKGTSLPGAQVTTLNHGAKLTNDNGEALFSNVPEGEISCMVYKENYINNVAGV
                     NLTSDSTVTVLLYANRYTATFFVTSKNVPVYNAQVIVEGIDTAYTSATGYANINLIPF
                     GSNIRYSVKLNGYNTYLGTFNLEDRNIVVNVPLTLTTIEPTKTNSFAIYPNPSNGNFS
                     INLEGSFSVAIYNLAGYKVYLSNHAENSLQVDIHSLPAGIYIVRVTGKNFSTVAKIIK
                     K"
     gene            48517..49836
                     /locus_tag="PK839_03050"
     CDS             48517..49836
                     /locus_tag="PK839_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013548070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOG71746.1"
                     /translation="MKETPIPREIVKQKIAESNLPRVGRGSIREIVHLVNEIEKASGQ
                     KFIRMEIGDPGLQPAEIGIEAEISALRSGVASKYPLVDGIPPLKKEISRFVKNFINID
                     VDERGCIPTVGSMQGGMAAFLMTTRCNTRKNTVLFIDPGFPVQKQQLKVLGIDYETFD
                     VYNFRGDRLHDKLESYLKKGNISTILYSNPNNPSWICFTEKELRIIGELATAYDAIVV
                     EDLAYLGMDFRKDLSKPGQPPYQPSVANYTNNWLMLISSSKIFSYAGQRIGCIVVSNE
                     LYNRHFPDLKRFFSSDEFGYAMIYGAVYPLSSGVCHSAQYGFAAMLKAANDGVYNFVN
                     DVREYGEKAKVMKKLFTDNGFKIVYDRDEGEPLADGFYFTISYPGLTGEELIEEMLYY
                     GISAISLLITGSERSEGLRACVSQVQRSQFSDLEYRLKKFHEDHPTK"
     gene            49887..50123
                     /locus_tag="PK839_03055"
     CDS             49887..50123
                     /locus_tag="PK839_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009120596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOG71747.1"
                     /translation="MAKIRGAIVVDVERCKGCSLCVVACPSSVIALAKEVNGKGYNYA
                     YMVNPDACTGCVNCAIVCPDTVISVYKVKADAVA"
     gene            50165..51250
                     /locus_tag="PK839_03060"
     CDS             50165..51250
                     /locus_tag="PK839_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008648284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HOG71748.1"
                     /translation="MAELKLMKGNEAIAEAAIRCGCDGYFGYPITPQSEIMETLMARQ
                     PWDETGMVVLQAESEVASINMVYGGAACGKRAMTSSSSPGISLMQEGITYIAGSELPC
                     LIVNVVRGGPGLGTIQPAQSDYFQAVKGGGHGDYKLIVLAPASVQEMYDFVDLAFELA
                     FKYRNPAMILSDGVIGQMMEKVQIGDYKPRLTDEEVVKRYGSWATTGKPSTRERNIIT
                     SLDLDANSQELFNLKLLKKYAEIEKNEIRFEEISCTDAEYLLVAYGSSSRICQKTVDL
                     AREKGIKVGLLRPITLYPYPYGAIQKLVPQLKGILAVEMSAGQMVEDVRLAVNGKVPV
                     LHYGRMGGVIPTPNEVLKVLEDKLIKG"
     gene            51255..52016
                     /locus_tag="PK839_03065"
     CDS             51255..52016
                     /locus_tag="PK839_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016276836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOG71749.1"
                     /translation="MDVKDIIKEENLVYSKSKVLTDTIMHYCPGCTHGVVHKVIAEVI
                     EELGIQDKTIGVSPVGCAVFAYNYLDIDWQEAAHGRAPALATAINRLYPDKYVFTYQG
                     DGDLASIGTAEIIHACNRGENIVVIFINNGIYGMTGGQMAPTTLIDMPTSTTPYGRKV
                     DLNGYPLKITDLVAQLPGTCFVTRHSAHTPAAVRKLKKAVTKAFQNTRRKKGVSFIEV
                     VSTCNSGWKLSPVESNNWMVEHMFPYYPLGDMKDE"
     gene            52044..52589
                     /locus_tag="PK839_03070"
     CDS             52044..52589
                     /locus_tag="PK839_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002561683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOG71750.1"
                     /translation="MTEEIIIAGFGGQGVLSMGKILAYSGIMQDQEVSWMPSYGPEMR
                     GGTANVTVILSDSRISSPIIQEFDTAIILNQQSMDKFEPMVKPGGLLIYDPNGITRHP
                     VRKDIAIYQVEAAEEAARTKNPRTFNMIVLGAFLKVKPIVKLDNVIKGLEKSLPERHH
                     NLIPVNKEAILKGMELAKKYS"
     gene            complement(52752..53360)
                     /gene="nadD"
                     /locus_tag="PK839_03075"
     CDS             complement(52752..53360)
                     /gene="nadD"
                     /locus_tag="PK839_03075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HOG71751.1"
                     /translation="MKIGLFFGSYNPIHIGHLVIADFILEFSDLDEIWFVVSPQNPFK
                     SIDELAADEHRLNMTRLAIPEKNTRMKVCDVEMGMPRPSYTIDTLRRLEQESPGCTFV
                     ILLGSDNIAGIHDWKESAALINGWDFYVYPREGSFNPKNLPSGRFTLIDAPVIGISST
                     QIRHLIAHGVDASGYVPDDVWDYIIKNKIYGYGTTLTANADK"
     gene            complement(53357..53932)
                     /gene="gmk"
                     /locus_tag="PK839_03080"
     CDS             complement(53357..53932)
                     /gene="gmk"
                     /locus_tag="PK839_03080"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HOG71752.1"
                     /translation="MDGKLLIFSAPSGSGKTTIVKHLLDCFPELEFSVSACSRPPRKG
                     EINGVDYHFLTVEEFKKRIAGNEFVEWEEVYPNLFYGTLWSEIHRIWNKGNHVVFDVD
                     VKGGLNLKKKFPDCSLAVFVMPPSMEDLKQRLIKRGSEDDESLALRLNKAKEEIGYAS
                     QFDVVLINNQVEKAFMEAERIVRDFIKIDQT"
     gene            complement(53984..54865)
                     /locus_tag="PK839_03085"
     CDS             complement(53984..54865)
                     /locus_tag="PK839_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002562461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HOG71753.1"
                     /translation="MLVSMTGFGRAEMQLGSKKLIAEIRSLNSKQLDLSIRIPSIYRG
                     IEMDIRSIIAQQLVRGKVDFQLSFEETAESATTPINRDIFEGYLLQIKEIARQFEIDI
                     TNESLVQTILRLPDVLRVNGKDVVGEDELNSVKKLCSNAVESLVNFRRQEGSVLYNDI
                     KNRVLLILDLLQKVEPYEKQRIEDVKKRLREALKTLEDDVKINSDRFEQEVIYYLEKL
                     DITEEKVRLKNHCKYFLETAGLDEQVGRKLGFIAQEMGREINTMGSKANHSDIQKVVV
                     QMKDELEKIKEQSLNIL"
     gene            55095..55451
                     /locus_tag="PK839_03090"
     CDS             55095..55451
                     /locus_tag="PK839_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HOG71754.1"
                     /translation="MAILLLVISNTFMTMAWYGHLKFKELKWFQNFGIVGIVLISWGI
                     ALFEYFFQVPANRLGYKENGGPFSLLELKVIQEVITLLVFIIFTVLFFKTESIRINHL
                     IGFCFMVLAVYFVFKK"
     gene            55864..56259
                     /locus_tag="PK839_03095"
     CDS             55864..56259
                     /locus_tag="PK839_03095"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HOG71755.1"
                     /translation="MDSLYIEAEIDSPEVYFDPETKIFSISGISHPENAKEFYQVIME
                     WVDEFYEYMKNKKPCKIIVDMNFKYINSSSYKYLRELLIKMSNFKNSHFDIEVVWNYQ
                     EEDEDLLTEGIVLFELPEINLPYRCVSYR"
     gene            56555..57643
                     /gene="hydE"
                     /locus_tag="PK839_03100"
     CDS             56555..57643
                     /gene="hydE"
                     /locus_tag="PK839_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HOG71756.1"
                     /translation="MAFIEELLAKESYTKSELVELLQVTGDGRAKLFSKAAGIKEKYL
                     GNKVYLRGLVEMSNVCRKNCLYCGIRSGNQKVNRYNLTDDEIVDAAIFAWKNKYGSIA
                     LQAGEISTREFATRVEGLLGKINAATYGELGITLSLGEQNEDTYRRWFQKGGIRYLLR
                     IESSNQELYKKLHPNDSLHDYKTRIDCLKSLRRVGYQVGSGVMIGLPFQTLEHLADDL
                     LWMQEMDLDMVGMGPYVEHPDTPLFEYRDQLLPVAERFDLSLKMTALIRIMMKNINIV
                     APTALQAIDPLGREKAIKVGANVVMPNITPGAFRNDYKLYENKPCVDDAADDCKNCLD
                     ARIQLAGHEVGYGEHGDSPHFYQRKNAQ"
BASE COUNT        17086 a        12814 c        12086 g        15659 t
ORIGIN      
        1 agcgccccgt tctaccattt cggcaatggc cttttcacca tcatctggat tcaggttgac
       61 cggcataacg gcatcaatta caacatcaac cttaaaacct aacgtaatag cgtcaaaaac
      121 agtattctta acgcagtaat cggtagctaa cccggttaca acaatatgat caacatccat
      181 atcgtgaagc catgatgcta atccggtttg ctgaaaccct gagtatgctt ccttttcagg
      241 taaagtggct tttgatacca gtatggcaac ccctgggaaa tatagatctg catgtagctc
      301 tgctccatgg gtaccagcga cacagtgcac tggccatatg cccccgtatt ttttgaacga
      361 tgaatggttt gccgggtgcc agtcgcgggt aaagattacg gccattccct ccgattcaaa
      421 tagctgcgac agcttgttga tgggttcaat aacaatttct cctttcgcta cagccaacgc
      481 tccacccggg cagaaatcat tctgaacatc cacaactatc aaggctttca taagaataaa
      541 atttagttgg ttgatataaa gggctgcagc ctggaattag ggctatactt tcgtcctacc
      601 ttagccattg gttcgtcttc gaccaaaaca atatcggcag tatagtcata gctgccctgc
      661 atcaggtagc tccctacacc gtaggcatca accggaaccc ccatactttc gaacatgcct
      721 attcgctcag gtgtaaaacc accggaaacg acaatcttta catgcttaaa accctccttg
      781 tcgagggcta aacgtacctt ttccaccagc ttgggattta ctcctgttgg tttaaaagtc
      841 cccatttcgg ggaaaatgct tttatcaacc aagttctcgg aagtatcaag cctgactccc
      901 cataacttgt cgcccagctc gcgggcacat gtaatggagg tattcacgca atcgttatcg
      961 aagtcaacaa gcgcaataag atctgcgctc gggtaggttt catgaaacag tttagccgag
     1021 gtaacagtgc tccccccgac cgatgctata agggcatggg gtattgttcc ggaagcgcca
     1081 gctccccacc actcaccttg agcatcagtt gaaacaccgg aagcaccgcc aacaaaagca
     1141 gcatacccat caccaccctg cacagcccaa tggtcgaaac gagctgggaa gtaaagaact
     1201 gtttttccat tagcagcatt aactacacgc cttacgttgg tggcaactct tgtccgacga
     1261 gcaagaatgc ccaagtatat ggtttcgaga tgggcaaaaa gcgaagcatc accatggata
     1321 tgcatagccg tttcgtaggg tgaaatggca tcgccatccc aaagtgcatc aattttcaaa
     1381 tcggaaaacc cgtcaaccca caacttatca agagcctggg aaactttcat tttctcctcc
     1441 tccagctgta agtatttctt tttgtcagta agaaatactt ttcggcccat ccgcttaagc
     1501 tcaataagct tatcaaacag cttatacgcc tgatcgtagt cggagtaacg cccactggca
     1561 acctttagca ccgcaactgc ctcatcaatg ccacacacta ctgcctcatt tttctgaaaa
     1621 acctgcatgg taacatgcgg gtggaggtta tggctttcaa gcacaacctt ctcgcgccaa
     1681 aaatacacat cgcttctata accccggcga agctcttgaa ccggaaggtc gaaggttgtg
     1741 ctatgcaaac gctccttcat tattcgtgca ataattaaat tcaacaaact ttaagcaaaa
     1801 gcatttaaaa taataatagt ttaaagggtt agctcatttt ttgccttccg ttcaacaact
     1861 actccgatta ttggaacttt taccaccggg gagttagcgg catttgattc gatggttaca
     1921 gttcgactta tgcgctgagg acgattttca attctgaagt aaacctcaat cgtccccttt
     1981 tggttgggca aaattggcgc tttaggccaa ctcttaatat tagttccaca gcatccggaa
     2041 accctggtaa gtattaaagg tttattaccc tggttcacca cagtaagctg caccttcccg
     2101 ttgccatcgt ctagctcatc taaaaaaatt tctcccagct ttaccgtatc tggaatagcg
     2161 ccaataattg gtccatgctt aattccatct ttatccattt taacatgctg ccctagcata
     2221 accaatggac ttaaaatcat gataaaaact attcctaggg atttcatata attcttcata
     2281 tacctaaata aatgctatct tcggattttc atactacgaa acaaatttac aaaaaaatag
     2341 ttcttttccc acgttaacac tacagaatga atttatggta aaaaaagcct gttgaaaggg
     2401 ctatcggcaa gcacaacagc atagattggc tgaccagctt ctgcctaata ataattttag
     2461 gtgatagtgt tcttaattat ttgggttgat gaaaacattt tcgaaagcaa ttgatgccgc
     2521 catttttgat atggatggcg tgttggttaa taaccaccag taccatctga atgcatggaa
     2581 agagttttgc aggcgtcatg gctataaatt tgatgagaaa accttcacca cggcatactt
     2641 tggaaaaaat aaccaggaga taattagtga gctaaccgga aagtctattt caacccacga
     2701 ggctgaacta ctgggagagg aaaaggaagc tatttatagg gatatttact tgccattcat
     2761 aaaacctgtt gatggtttga tcgagctgct agattattta aaagaaaacg gagtaagact
     2821 cgccgtggca acctcagcac caaagtctaa ctttaaattt attgagaagt cattacgcct
     2881 cgagaataga tttgatgtgg ttgttgacgc atcaatggtt cagcatggaa aaccagaccc
     2941 agaaatctac cttaaagcct ccgagctact aaaggttcac cctgaggggt gtttagtttt
     3001 cgaagattct ttttccggaa tccaggcggc cacagcggca gggatgcggg tggtagccat
     3061 ggcaacaact catacccgaa atgagcttgg taaaaatctt acagtagctg atagcttcta
     3121 tgacgtgcta gccttcattt taagggaaaa cgagtagcca tttcagctgc agccactaga
     3181 ttggaagaat gaaccaaaat ttcaaagtgc cattgatact atctccaatt atggctatat
     3241 tgcctatcaa tatattatta aataagcaat cacaaagtaa atatctttac atcaaccacg
     3301 atggctataa atggtgaagt tttaagcctt cattttaaca aacaaatatg attggcatat
     3361 gaaaagatgt ttagttgtaa gcctaattgt tttgctagct gcttcggcta aaggtcaaaa
     3421 taaagaagaa gacattttta acctctcgct ggaggagctg ctgaacttgg aggttagcat
     3481 atctactaaa acagcaacaa atattagaaa tacaccggga atacttacgg taatcaccag
     3541 tgaggatata aaaaacagtg gtgtaaacgg aataatagat ctttttcaac tttttgtgcc
     3601 aggtttcgac tttggggttg atgttgaagg agtagtagga atgggagtac ggggtatttg
     3661 ggcacatgaa ggtaaagttc tgtttatggt aaatgggttg gagctaaacg acgggatgtt
     3721 cgcatccgtc ccattcggta accactttcc gttgaataat atagaacgaa ttgaggtgat
     3781 aaggggcccg ggctctgcaa tgtatggagg ttttgctggt atgggtgtag tgaacatcat
     3841 caccagggat gtatataaac cgggaggata cgtttcttac actgccaacc acacaggcag
     3901 gcaattttca caaaataacg taggctttgg tacaggcgta caccaaaact accttaacat
     3961 aggtatatcc ggaaactacg gtaatggttc gcgaagcgac agggattacg ttgactacca
     4021 tggacaatca cgttcaatga ggaatgctag cgatacctac acccgccagc tcgacctgaa
     4081 gcttaactac aaaaacttca acctggttgg gtttattgat gactactcct accagcaaat
     4141 cgacctctgg gatagctact acccaggacc gcctttaacc gaaagcttca agagctcgct
     4201 ggttcaagct acttatgatt ttaagcctaa tggcaacctt accattactc caaaaatctc
     4261 ttacaaatgg cagcagccct ggaaactcac tgccctggga gctggctatg ggtataacaa
     4321 caacaaaaca tttgatgggc ttttaggagg aatttcaaca agctacacaa aaaataagct
     4381 gaccattgtt ggcggagccg agctcaaaca tgatcacctt aaccagcccg agctggttga
     4441 cacggtttac gaggaaacat ttaggaatgg caaagagcag ctgtcctaca caaatacggc
     4501 tatttatggg ctcttacaac acgatagccg tgtaggaaac tttactcttg gagccaggta
     4561 cgatttttcc agcgagtacg gcagctcgtt tgtaccaaga gtaggcattg ccaaggtttt
     4621 tgattttatg cacttcaagg taatggtgag ccagtcattt agggtccccg gaggaatcct
     4681 tcccaaccgg ttgccacaag gcaatccgga aatcaagcct gaaaaaggaa ccagctacga
     4741 agtggctata gggtttaaaa tgccttttca atcctacatt acctttaaca ttttcgatgt
     4801 atctttcgat aaggtaattg tttacaactt gggtaatgat gatataacag gagtaggtta
     4861 ctataaaaat gaaggtacaa ttggcacaag aggtattgaa gccgatttta ggcatatcag
     4921 cactaaaatt tcatcatttg ctacattcgc ctactacatg cgtaaacatt ccaagaccga
     4981 tagcctttac agggtaccgg acagggaaag ttacttttta ggattttcgc ccctaaggct
     5041 taacggtggt tttaacttca gggtaaacaa ccgtttatct ctcacgttgt caggatctta
     5101 ctttggaaaa cgttacgcct ttacccaaag ggaaggaaat ggaatggata tactcgaaaa
     5161 actgaaccct ctttttatgc ttggctgcaa tgtaaacatt aagaatatag tgctaaaggg
     5221 tcttactgca actattgagg taaacaatat gctgggggaa tcgtttgtat ttccccagcc
     5281 ctataagggc tcacatgccc ctttacccgg acttgacagg agttttggct ttaatcttgt
     5341 ctatgaatac taaaacttaa gtaaaaatgg ccctgctagg gccattttca tagtgaaaag
     5401 caataggtaa aaaagttatt gctaactttc agcatcttgg gctgtgttgc tatgctcttc
     5461 agctgccggc tcttcaggtt ttgcttcttc aacatcgaga attgctaaaa gattgtttgc
     5521 ctgaagctgg ttgtaccagc taagaatttt tttaatatcg ctggtataaa ccctctccct
     5581 atcaaaatcg ggtaaaacct tctcgaaata ctcccgaatg gccttatcag tcgaagtttt
     5641 aggatttaaa gtgggtttag aatcttcgag gttacgtatt agcttaagaa cctcgataag
     5701 gggtaaatcg gtggaattag taaaaaccga tatgtcggtt aaggcggtaa cctttgaggt
     5761 aatgggggcc gtcattcggt ttccatcagc aatggattca acaataaccc ctttccgccc
     5821 ttgcgataca attttgaata aaccggagta gcccgaaata gaaatgattt ccttaagtgt
     5881 agtttccata cttcctagtt atattttcgg tgtaaagtta agttgaaaaa attttaaatt
     5941 aaaaactgcg taccgtttaa atgaacaata cacaaaaata gccggcgtct gggtagctga
     6001 aagtttaccg gttgcttttc cggccataat ggaattttgc ccacaccagg taaaaggttg
     6061 cagcaatgtt aaccaggtag tttaaccaca atggccactg gtattttccg cttaccatat
     6121 cgttataagc aatgtaaata aaggtaaaaa aaacaccaag agcccataac agaagaaaaa
     6181 cccagttaaa actcgacacg ttatgcgatc gccacgtttg aacaacctga gggataaccc
     6241 caatgctcag aataatattg ccaaaccatc cgatagcctc aactgcttcg ttcatattgt
     6301 gttgttaatt aaaaccccgt tcctttttaa tcttctcgta ggcttcgttc accattttaa
     6361 atttttgttc ggcagcttgc cgtacatcct ctcccaaatg gcttaccttg tcggggtggt
     6421 attttatggc catttgtcgg taggcctttt ttatctcatc gttagcagca gagggtgaaa
     6481 cctccaaaat tttgtagtac ttatcggtat cgggaataaa catggctttt atggagtcga
     6541 agtcagctgt ttcaactccg aggtattgag caatttcatt aataatttgg agttcagccg
     6601 ggcttacaac tccatcagcc tgagctattc cgaaaaggaa gtgcagcatt tgcaaccggg
     6661 aatggtaatc tacattctcc ttaacctgct tgcacacatc cttcacagga accgattgct
     6721 taagtatatc cctcagcaaa agaatagcct cccgggaagc cgaggctcca aaatttttgt
     6781 aaaagtagtc ctttacgtag cttagctccg attttaacac cctgccatcg gccttcatca
     6841 cagccgaaac cagcacaagc aagctaacca ggaatccatt tcggctggtt tcgcccgtat
     6901 agattttagt aggggtttgg gcgctgtcga atatagagcc aatagcaaat cccaggatgg
     6961 ccccaattgg cccaaataaa gcccagccta aaccgccgct aatccattta ccgtatttac
     7021 ccatttgttt aaagaggttt aagcctttcg actatttcaa taacctgggg taaaagtaga
     7081 gatttttcat cattattaat gataaagtca gccagcttaa ctttcaattc atcatccatt
     7141 tggttgctca ctcgtgcttg aactgcttcc ttgtttacct tatcgcgagc cataaccctc
     7201 ctgattctta catcagcagg tgccacgacc agcaccgttt tatcaagttt tttgaaaata
     7261 ccagattcaa agaggatggc cgactccatg aaagcaacct ggttatcctt gtgggtctcc
     7321 gaccattttt caaaatcgtc cattaccacc ggatgtataa tgctattgag ctgctgcaat
     7381 agcatgttat ccgcaaaaac cattgatgca agccgctgcc ggttgagctt gccgttgcag
     7441 taaatttcct ctccaaaaag cagcttcacg cgctgcttca ctccttcatc gctatcagta
     7501 attgtcctgg aggctatatc agaattatag acaggatatc ccaatctggc aagtatgctg
     7561 caaaccagcg attttccgct tccgattcct ccggttaccc caaccatcag gctcatttct
     7621 tcttcagaat atagtcaaca tacaagggtt caaagctaac ccgactgaca aaatcggggt
     7681 ggttttcgag taaaaccttt agcttgttgc cctccattga accggtgtcg gatatgttgc
     7741 ataccgccct gaaatttgaa ggttttaact tgaagtactt gctcaaggtt acgttacacc
     7801 taagctgaat ttcggccggt aataaaattg catcgtagct gggaggtacg ttaaggggga
     7861 taatcggaac attaagcata acctccgtaa acttttcaac gggtataaat acctcaacct
     7921 ccgaatttgg catgaaaact tgttttacct ggggaatgtt caatataatt ttcttgtttc
     7981 cagcaaggag atttaggctt acgggttggg ttttgagctc gttgatggta tcgagaatag
     8041 cagcaggccc ggaaatagtg atttcaccag gttcaacctc aatgtttccg gcaagcatgt
     8101 actgtcgtgc aaaagtcagg ctaaggttgg gaacaaccct aacatttttc tccacaacat
     8161 cctcaagttc aaggcaaatg gaatcgggga aaacgtccag cacgtccata tccttaccaa
     8221 gttgcgctga aatggaagaa aattgttgtg tagttgaaac acaaaattgg ctggatgaat
     8281 ttttgccttg gttaggtttt aattctttta aatcaatatt aagggggtag ctgtagcttg
     8341 taaacttata cctaaggagg gtatagccgt atgctttaac ctttacgcta attttctcgg
     8401 ggggggtgcc tgtaataatt tttcccttct ggaggtttga ataccttaca ggatatacca
     8461 gctcatcgga gtactcgtgg ctaagcttta gaaggaacca gagtatggca gaaataacca
     8521 aaaaaaggga aaataccgcc aagcgctttt taaaattagc cttttcgcga tatattatct
     8581 tgttgaactg tttgagtagg gttgaatcca actttaagga agtaaatgca ggattcgttg
     8641 cccaaggctg aatcctgcaa tgctttcatc aaggttatag ctatttggct tgaatatcgg
     8701 aaacatcacg aattattgtg cttttagcaa ccttaacgtt gatgttatca gaaatttcaa
     8761 ccataacgta atcatcctta acctcgctga ttttaccgta aacacctccg gcaataatca
     8821 ccttatcccc ctttttcagg gcttcctgaa acttgcgaag ttctttttgg cgcttcattt
     8881 gcggacggat cataaaaaag taaaatacca cgatgatcag tgcaaacatt aaaatagtca
     8941 ttaaggggtt agccccttgt gcagctggtt tttgcaatag tacgaatagt gtttccatct
     9001 ttaagtgttt agtttgttaa gcattaaatt tcgaagcaaa ggtagtattt ttttaagaag
     9061 caacctatgg catcacgacg tttgctctga tagtaaccga tttgtccata atggatgaat
     9121 ttgtccgaag ggtaatcgtt ttatactgca atccggccca tccctggctg ttgaaaataa
     9181 cttcaacggt tccctcctca ccgggtttca aaatggtgct gcttagctta gaggtagtgc
     9241 atccgcaacc aggaattaca tccttcacca ccaacggggc atttccggaa ttcttaaaac
     9301 ggaatgtgtg ggttaccact tctccctgag taattgtgcc gaaatcaaaa aaatcttcga
     9361 tatactccag gtttggaacc cctgagctgt taccagaggt gctcagatta tttttaggtt
     9421 tggagggcga gccacaggtg gtaagcagca gtacacacat aacaaccatt cctcttgttc
     9481 tcataccaga atatttagta atttttagct gaacttgttc tataaacctt ttgaatttgc
     9541 aaccgaaaac agctgaaatt gctacttcag ggtgcggatg ttggtggttg ctgaatttag
     9601 cctggctatt cggtatctgg ttctaagctg tttatgtcat caggagtttc ggctatatcg
     9661 ccaataagtc ctctcccctg cttctcgaat ttcttttgct ggcggagtgt ggtaattatt
     9721 ttatcgagta caccgtttat aaaaagtccg ctttgctccg tgctgtagaa ctttgcaatt
     9781 tcgatgtact catttaggct taccttaacc ggaattgatg gaaattcaag aacctcggta
     9841 atagccattt ccatgatcag catatccatt gacgcaatcc ggtcaacctc ccagttctgg
     9901 gtgtactcct caatgaggcc aacattatca gaatgcttga gtaccgcttt tgtcattagc
     9961 tgaaaagcaa agtccttatc ctcctcgttt cggtataaag gcatcaactt tagagcatca
    10021 ggattttctt cccttacctt tttaatggtc ttagcagcca tgctaagcac aaacttcagg
    10081 tcatcgttcc agtagatgct ctgctcctcg agaaaagcat caaattcatc agaattttca
    10141 agctcgttta ggtagaagtt aacaatcatc tgcctgcagg tatcgaggga aaattcgtcg
    10201 tccgacatga atttatggta gctttcggag gacaccagct gctggtagag aagtttaacc
    10261 acctcggggt agtttttcca gctcaacttg gaatcctttg ctttctgctt gagcgcttga
    10321 ttatttctga gaatatgaat tagcggattg tcaacaaaac gggtatttgg atttagctca
    10381 tcggcggtag gacggaactt ttgtttaccc agctcaattt tctccagggc aacatcggcg
    10441 agtgcttcag gaagtaatag cactaaaagg taaagttgat aggacttttc aatgctaaaa
    10501 tggagctcct tttcaaggtt gctaagcgaa tcgttattgc ttcggaaata cgcaaaaaga
    10561 acttgtagaa tttttatccg gagtaagcgt cggttaatca tttaagaaca gattttcaaa
    10621 gtaaaaagcc tacaaagtta aaacaaaata gcatgagtag ctatattaga tataaaataa
    10681 tcttgcggtt aataaaatat aaaacaaaac caaaacattc tggtaaataa ttttttaaaa
    10741 tcaaaaggtt ggatagacga ttaatttttt atttttgatg gtcataaaag cattgtttat
    10801 ccttaaacta aaactcttac agtgatgacc gatcaacttg ataatcaaaa tgaatttgac
    10861 agggcagaaa ttgaagacct tttttcaaat gtggtaaaag ctggcaagcg tacctacttt
    10921 tttgatgtaa aagcgacccg tagcgatgag tactacctta ccattaccga aagcaagaaa
    10981 aggatgggca aggatggcaa tatgttctac gaaaagcata aaatattcct gtacaaagaa
    11041 gattttgata actttatggg tggtttgact gaagcagtca acttcatcaa gtcgaaccag
    11101 gaagtgcttc cccgcatagc ggcagagtat ggcaacgaag cttcactggc cgaggtaaac
    11161 gatgagctta ctacgttgaa ctttgaggac ttagatacaa agtagataat taaactagag
    11221 catgcaagcc ggctatttgc cggctttttt gttatagttt tccgatataa atttaagttt
    11281 agtaggtgaa tccggttata tatacagatc aaaaagttcc gcatctgtaa gctgccggtt
    11341 ccccaacttg aatttttaca aggaaactaa taacaatatt ggtggtgctt ttgaatttac
    11401 ttgtaggtat gatcagggtt gtcgtcaaaa taaattccgt ctatgggctc cctgttgagc
    11461 actttacgta tttcatcagg cgccaaagtc ttaacattgg taactatata gccctcgtcg
    11521 agcaggttgc ccatgccgtt acactcctta attaccgccg taacaggttg atttacagga
    11581 acactcatgg gtactagagc agtaatgcac atgtaccgat cggccatttc aacacgctcc
    11641 acttcagaat cgtagtgctt gaaaataaac ttaacctcat ccgtaaccag tttagcctgc
    11701 tgctggctta gcatgtcaac agcaggaata agcacaataa aatagcgaag tttcccatca
    11761 agtccaccca ttcctgtgct aataagcacc ccttcctcgt cagtcagggc acctaccaga
    11821 tgaaacctgc actcgtttaa ggccagcaag gcccatccct ttagctggga cggggcaaca
    11881 gaaacaaact tttctattgc cctgtagctt tctacctccc ctgatgaggc taaaagcacc
    11941 aaaacttcct ttagctgctc ttccgttgta tcgttcgaga ataaaatatc ggcagaaaca
    12001 tttacctcct tagtgtcttg tttattacgg aattgttccg ccattctgaa gtaatccttt
    12061 tgaatggaaa tatcaaccac atcctcaact atgctcaatc gtttaccggc attgcctaaa
    12121 aactcctgaa tctgcctaaa aatctcttcg ggttcatttg cttccatacc agaataaaat
    12181 tatttaacgt ttgtaaagca gcttattttt aacagcgcac atcgtgccct gagttttaag
    12241 ggagttgaca atgtgaatgt tgccataaat agcattactc acgttgcggc ttttaaagtt
    12301 ttggctgaat gttatatttg tgctccattt aaacaattat gcgaaatcaa agttactaga
    12361 atattataac aaaaaaatgc agctatcgga gcttcataac ggggagtacg ggattatttc
    12421 caaaataagg ggacgcggag cgtttaggaa gcgtatcacc gagatgggtt ttgtgaaagg
    12481 taaaagggtg gatgtggtga agaatgcccc attgaatgac cccattgagt actccattat
    12541 gggctaccag gtttcgcttc gcagaagcga ggcttccctc atagaggttg taacccccga
    12601 tgaagcgaaa aaaatattgg ccgaaaacca caccgagcct ttaactaccc taacagaacc
    12661 ggaactggtg caaaccggct tgaaggtgat gggaaaaacc atcaatatag cccttgttgg
    12721 gaatcccaat tccggtaaaa ccaccatatt caacttcctt tccggttcaa gagagcacgt
    12781 gggaaactac agcggcgtta cggttgattc caaaatagcc cattttcagc acaaggggta
    12841 caccttcaac attaccgacc taccgggaac ctactcgcta acagcttaca cccctgagga
    12901 ggtttacgtt agaaactaca tccacgaaaa ccatcccgac cttattatca acgtagttga
    12961 tgcctcgaac ctcgagcgaa acctttacct caccacccag ctcatcgaca tggatgtgag
    13021 gattgtggtt gccctaaaca tgtatgatga gctgctggat aaaagggaca ggtttgacta
    13081 cgaaatgctg ggtaaaatgc taggcattcc gtttgttccc accgttggtt ccagagctaa
    13141 aggtttcgac aggctgaagg acaccattat aaacgtattc tcggaaaagg ataaaacctc
    13201 gcgtcatatc cacataaact atggagctgt aattgaggaa tcaatagcag caatccagca
    13261 tgaggttaga aaaaatgaga ctcttacggt taactactcc agcaggtacc tatcaataaa
    13321 gcttttggaa aaagataggc tgactgaccg catgctgaag gattttccaa attacactga
    13381 aatacgcaaa attgcccggc aagaggtaga aaggctagaa gcgctgttcc atgaggattc
    13441 tgaaaccctg attactgatg ccaaatacgg attcattgca ggagccctaa aggaaaccta
    13501 ccacgaaagc agcataaaaa aaaggcaaac aactgaaatt attgacacgt ttatcaccca
    13561 taagctcttc gggttcccaa ttttcatttt tttaatctgg ctcacctttt tcaccacctt
    13621 tactttaggt gagtacccca tgaactggat agaagcgggg gttgcatgga ttagtcgttc
    13681 tataaatggc ctaatgccca atggcccatt aaaggacatg ttagtagatg gaattattag
    13741 cggtgttggt agtgtaatag tcttccttcc aaatatcctc attcttttct tctttatatc
    13801 ccttctggag gataccgggt acatggccag ggctgccttc atcatggata agttgatgca
    13861 caagataggg ctgcatggca aatcgttcat cccgctggtt atggggtttg gatgtaacgt
    13921 gccggctata atggcctcac gaaccattga aaataagaat aaccggctgg ttaccatgct
    13981 tataactcca ttcatgagct gtagcgcccg gcttcccgtt tatatactga ttcttggaac
    14041 ctttttccca aaacatgccg gcaacctgtt ggtgctgata tactttaccg gcatactcct
    14101 cgccgttgga acggcattgc tgctaaaaaa aacgctattc cgcacggacg atgttccctt
    14161 tgtcatggag ttgccaccct accgaattcc aaccctacgt tcgatactta ttcacatgtg
    14221 ggataaagcc tcccagtacc ttaataaaat cggaggagta attctggtag gggttatcat
    14281 tatatgggct ttaggatact ttccacgaaa tgtacagaca tcaaaagatt atgacagttt
    14341 gattgggctt aaacgtgctg ctctaacttc agcacaagcc accaacgctg ccacagcaga
    14401 gagcctggag caagaaatcc aagaaatcga gatggaaagg gaagccctgc gacaagaaca
    14461 gtcgtacctt ggaagaattg gaaagttcat cgaacctgcc ctcaaaccgc ttggattcga
    14521 ttggaaaatg ggagttagcc tgatttcggg gattgctgcc aaggaaattg tggtaagcac
    14581 cctgggggta cttcatctgg ccaaagagcc aaatggttca caatcgctac ccgacaagct
    14641 taaggccgac acttacacta ctggggaaaa ggttggccaa aaggtattca cgccccttag
    14701 ttcattctcc tttttaatat ttattctaat ttacttcccc tgtgtagcag taatatcggc
    14761 tattaaacaa gaatcaggat cgtggaaatg ggctcttttt accattgttt tcaccactgc
    14821 gctggcgtgg gtttcatcgt ttctattctt tcaggttggg actttaatta caggaagcta
    14881 gtatggttca ggaaattgta gtatatatgt tgataatagc agcaatcctc tatgtaggat
    14941 acagcattta ccgggtattc aggccaaagc aaaagaattt gggccattgt gccggatgca
    15001 gctcctcatc ttgtggaatt agggagttga agagtaagta agctctacta attgagtgtc
    15061 aaacgaatgg cattacaaat tatgtgaaac ttaaaactaa atttatagca accatgaaaa
    15121 cagaacaatt ttcagtggga aagatgaagt gtatgggatg tgtagctaca atcaccagcg
    15181 gtctttctga aattgaagga gtatcaaaag taaatgccga acttgaatcg gcaactgtaa
    15241 cggtaaccta tgagaatgat ggtttacgag aggtaataat caataagctc gaagcccttg
    15301 ggtatccggt taaccttgat attaagcaag gctcctaacc gaaaaccatc tgcacgttca
    15361 tgtattcagg tagaggaagt gaaggtatca cttcctcttt tggtatccct taattacaaa
    15421 aacacctagg tttccgatta caaatacttt agtccatgct tttatgcatg ggaacttagt
    15481 ggttagaaca cccatgttga taattttact tctatcgtgt tagtattcgc aaaaacatgg
    15541 gcgtttcaaa tttcaagtag aaacatttta ccagtcaagc tgcctttacc cgtgtttttt
    15601 cacgaagtcg gggtacagct tggtttttac ccatttaaaa tcgggtgaaa tagccaaaat
    15661 ttccttaaaa gttttacggg ccaaatcaat ttttccagct ttctcagcag aaagtgccat
    15721 ttgcgcccag gcattcatgt accaccacgt tttaggatgc tgtccgttat taccttttcg
    15781 aagaatttcg gtagcttttt ggtagtagcc taaagcttcg gtagggtttc ctcccacaac
    15841 acccggagca tactgtaaaa aattggcctt ttctaaaagg gggtacggat tattggcatc
    15901 gagcttaagc gcctcatcaa ttatttttat gctttttggc cctaaaaaag gagccttata
    15961 tgggcttaaa taaactttaa aagccaacag ggcagaattc attgccatgg caccaacatg
    16021 tccaggttta atctttagaa tgttgtttgc atgctgctcg gccaagtcga tgtagcgttg
    16081 agccttttcc ttgtttccat ttgaaccaag caggtaggga ataaagccat actgggcatg
    16141 aagaagctga aattcatcat caatggttgg tttcattcga taggtgttct ccaaaacccc
    16201 tattaccctt tcccattctt ttatatcatc gtaaatgtag caccggaaga ggcgaatatc
    16261 cacaggttcg acttcggtag ctgaatctaa cctcccgcta cctgtgacca ttaacagaaa
    16321 agtaagcgca cctccaaaca agaaataacc aaaatgcttt gttctagcca taaacgttaa
    16381 taatacacta attagcttaa tttctatctt ttatgtcctg taatgctgct atgaaggggt
    16441 gaaagcatca ttctaaacaa aactagctgc tgaaggggaa aaagtagaat agccgaccat
    16501 gctgattgtt cgcatagagc tgctacgagc attcggccta atagcaccga aaaaagatat
    16561 gcgaaagtaa agggataagg catagctaaa agagttataa aaggcccaag ggtggtaaga
    16621 atggcatagc tgaacattag ccactctctg ccggaaaaaa aatcgaacaa cccacctgca
    16681 acccttcgca acgcataacg gtagcttgta aaatagctgc aggaaatttc ctcctttctt
    16741 cccaacagcg taaacgatcg gagtcgtaac tgcttaaact ccttgctgta catggcgctt
    16801 ttaaggtctt cgggaatggt cacagaggtt agagtatgcc ttctaaagac ttcagcgttg
    16861 tacatcaccg gcattccatc tgtcagtggc aacttagggt taaaatgttt tccaatcagc
    16921 tttaaaggta taagtgatag catcaaccac tgaacagccg aaacagcaat tttcacgcca
    16981 aaacttttgg ttgtatcata atgtggtgta acggaaagca cggctagctt tctgtgcttc
    17041 atgttggcaa ccattgcgtt aataaaatga atacccatcc gcatgtcagg atcaaggaaa
    17101 agtatgtagc tgcctgatga tttttcgaca agcacattgc gaaaattact cgaataggat
    17161 tcaccagctt cacgaatcgt tccatttaaa aacttaaacc ggttatcaac cctggcaaaa
    17221 gccaattggt catcgggaat actgttcaga ggactgctac aataaaccaa cacctcgaag
    17281 tttgtgtagg actggttttg caagtcgtgc aggaggttaa caagactagc tgtatttttg
    17341 ccctggccaa ccacaaccaa tacctttcca tggtctatca gctcaaaagg tcttaggtag
    17401 ggggtataaa tgtagttaac caaaacaacc accaacctaa gcactgcaaa aacaaccagc
    17461 aacaaaacca agtaaaaaat taccattctc tatataacta tcattataac aaagcctacc
    17521 ctatacaact ttaatttaca aaggtaaaac ttttcaatat agtgagaatt gcaaaggtaa
    17581 ggcctgctca aatacaatac cccaaagcat cattgattgt tagcttaaag atggagtgtt
    17641 attgtgcaat aataggcttt atagttatag gctacattat aggcaattct ttttcgacat
    17701 accacgctcc aatcatcatt aaaaaaaacg attcctataa gggaaccgtt tactaataca
    17761 cacggggcta tgttggcata gtctcagcat acaagcaatg aaattgacat tacatacgca
    17821 tttttttgaa ctcctccttc atgcggttga tatcggtaat ccaatcctca atgtcgtttt
    17881 cgtgctcaat ttcctcgtta agaatttgaa cggccatttg gtgggttgag tggtctttcc
    17941 cattggtgaa atcagcaatt tcctggtaac gtttaattgc gcaacgctcg cccttaaggt
    18001 tttgctccag aaccacctca atgtaggggt caacgggggc atcgtaagtg caacggttaa
    18061 ccttaaacca gtcctgtggg cttagtatag gcgttccgcc cagctgaatt attcttccaa
    18121 caacaagtac agcgtggttt agctcttgtg tggcatgaag caggagctca ggttcaattt
    18181 cgctccgcat ggggccctcc atcactcggg caccaatcca gtactggtag tatgcaagcc
    18241 actcctcggc tagtgcggca ttaagcattc ccagcagttt ttctaaatca accgatacaa
    18301 cttgtttggc ttcttttccc atagtaacaa tgttttttaa agtgtattcg ctactttata
    18361 cgacggacat aaagagtttg tttgccaaat agaaaaaggg ttggctggtg caacccgatt
    18421 gcctaaccgt taatgctaat ctggcctaac cttttagcat ccagaatctg aattttcctc
    18481 ttagtcatgc ttattaatcc ctccctgata aactcgctaa gctgacgatt aacagtttct
    18541 cttgaagtgc taattaaatc ggccagctcg ctgttgttga gcggcatttc gaaggtgctt
    18601 gagcaaaaaa gatcgtcgga catgcacagt agaacttcgg caacccttcc cggaatctgc
    18661 ttttgcgaca tgcttgtgca gcgacgatat tcgacaagtt cgtttcggca aagatccata
    18721 atgagttcat aggcaaaatc accattttta gtgataaaat ctttaaaggt gtcaagcgag
    18781 atgaagcaaa cttcggcatc ggaaatggct gttgcagagt actgatttat acgatcgcca
    18841 aaagtagtcg gaaggcctaa atatgtagga gctttaatga gttttaatat tttatcgccc
    18901 gaaggcccct tcatgtgtac ttttaccata cctcttttca ggtagatagt attggttgcc
    18961 aaggttccct ccctgaatac cacctcaccc tttttaaaca gcacctcggc gcagccaaac
    19021 tccaatgttt ggagttctgt ctcgttcagg tgctttgctg ctgacgactt gtaaccacaa
    19081 gttctacatt cgggatatga cataattctt cagatatctt ctgcaaagat acaaaggtta
    19141 aagatttatt gtgtgaaaaa tctcacagca aagcgtaagg gatatcacac caagagtagg
    19201 taaaataatg acaagcagct atacttttga attgttaaat atttaacaga ttataaccct
    19261 ataccacaat aagacatgga aatagatgtt gaaaagcagc tcgaagagct aaagaaaaaa
    19321 gtaggcaagc tcgaaagtgg tcggaaggac aagttgacta tggtagtctt ttccggcgat
    19381 atggacaaac ttttggcagc aatgatcatt gcaaccggag caacagcaat ggacatggag
    19441 gtaagcttat tcttcacatt ctgggctact gctgctctcc gcgatcccaa gaaaaaagtt
    19501 aaggggaaaa acctcgtttc aaaaatgttt ggtttcatgc tccccaaagg tgcaaaaaag
    19561 gttaagcttt cgaagcttca catggcaggt atgggtaaaa ccatgctgga gaacattatg
    19621 aaaaagaaga atgttgcctc ccttgatgaa atgtttaaaa cagccgcaga acttggcgtt
    19681 tctataaacg tttgcgaaat gtcaatgaat ttaatgggtt tcaaacgcga agagctaatt
    19741 gactatccca acctaaagat ttgtggtgta ggaacattcc tctcggatgc cgaggagagt
    19801 aaaattcagc tgttcattta atagtaaact taaacgatac cactatgagc gaaaaattga
    19861 tcgtaattgt tacagaagga cctgataacc aggaaaaggc aacaattcct tttgttatgg
    19921 caacagcagc acaatcaatg gatgttgatg ttactattat tttgcaagct gagggggtat
    19981 tacttgtaaa aaaaggtgtt tccgaaaatg taaacgcatc gggctttatg ccactaaagc
    20041 aactgcttga tacattcatt gaactaaatg gtaagctact tgtatgcacc ccttgcgcta
    20101 aatcgagagg gattagcgaa aatgatctag tcgagggttc caaacttata gccgcaggaa
    20161 cagttatttc ggaatcgtta agcgcaaacg ctgtattgag ctattaaaca caaatcaaac
    20221 aacaaaacaa tgaacgcaga agaattaaaa aatctaaagg taagccaaac cgttgatgca
    20281 cggggtacgg cttgcccagg tccccttttg gctgcaaaaa aagctattgg agaaattgaa
    20341 tcgggtgaaa tcatggaagt actttcatcc gacgaaggaa caaagcacga tataccaaaa
    20401 tggtgcgaaa aaatggagca tgagttcctg ggaattattg aagaggatag cttcacccgc
    20461 ctatttctaa aaaaggctta gggtgaaaag gataaggaag cagggctttt taagccttgc
    20521 ttcttacttg tacaacaata ttttttctgc ataaaacata gctatcatca tgttaagtga
    20581 aacattttca ccaaaaattc tggtcttctc caccgacata atatcggacc cggggatcga
    20641 ccaagccgga ttacgtaagc tgcactaccc ccctactgtt tacgtaatta gcttgccctg
    20701 ctcatcgggc attaaaccga gatggattat gcgggcgttc gaaaaaggtt tcgacggtgt
    20761 ttttattgct gctgatggcc atgagtgctc ctacagccca aaatgtgctg agcacaccaa
    20821 taatattatt gtagagtcgc agaagttgat gaaggagaac aacattaacc caaagcgaat
    20881 acgtatggct gctttatgtt ctgtttgtgc tgagccattt gtgaatcata tgggaaactt
    20941 ctcgaaaata ctctcagaac tgggaagtat tggtaccgaa aaatacaatc caagttaaat
    21001 ctttcgtgca cttcgtgaaa tcctttgagc cactttgcgg ttaaaaattt aaccacaatc
    21061 aacactaagt acacacaacg tttcacaaca aaaaattaaa gatttatttt aaatcttaat
    21121 cgatgccaga aaataaaaaa acatttgatg cccttgtagt gggaggtgga atcgcaggtc
    21181 aggaggcagc gctaagttta gccaatatgg attacaaggt tcttctagtg gaaaaagagc
    21241 tttccattgg tggtaagatg attcagctta gcaaggtgtt tccaaccctc gactgcgcag
    21301 catgtatcac tacaccaaaa atgtcggaaa cggccaggca tccaaacatt cagttactgc
    21361 tgaatacaga cataatggag attactaaaa ataatggatt attcaatgtc aacctaacca
    21421 ttcaacctac atacgttaac cctgatctat gtaccggatg tcagctatgc gagtgggcat
    21481 gtccagtagt aatcgttgac aaatacaaca acaatatggt tgggcaaaag gccatatcca
    21541 tagcctttag cttagccaac cccaaggtag ctgcaattga catgaaccac tgcattcact
    21601 gtaaaaagtg cgaaaaggtt tgtgaaccta aagctattga ttacgaaatg caaccaaaaa
    21661 atataaacat tgagacaaaa acagtgatta ttgcaaccgg gtttaacctt tttaaagggg
    21721 agaataaacc acaatatgga tttaaaaaat ttcccaacgt aattgacgcc atgcaaatgg
    21781 ataggcttat tgctcctact cgaccctaca atgcagttct acgtccaagc gatggtaagg
    21841 ttcccgataa aattgcctac gtactttgta cgggctccag ggattcaagc cttgagaatg
    21901 ccataggatg cggggctgag tgctccaaca acccaatatg ctcacaaatt tgctgtatgt
    21961 actccattaa gcaagcccag ctactaatgg gtgctctccc gttggccgat atcaccatat
    22021 actatatgga tataagggca tttggtaagg gctacgagga gttcttccag caatcaaaag
    22081 gaatgggggt caactttatt aaagggaaag ttgccaaaat caaccaaaag gatgacaata
    22141 gtggcgattt aattctccga tacgaggatg ttacaaccgg agccctcaag gaggctaatc
    22201 acgatttggt agtgctttcc gttggggttt taccaaacaa cggaataaca aaagttttta
    22261 ctgatgataa attagagctg gataatcagg gctttataaa atcgattgac gaacttgtaa
    22321 gcccctcgtt aaccagtatc gatggtgtat ttgttgcagg taccgctgcg ggtccaaagg
    22381 atattcccga ttcaatacta tctgcaggaa cagcggcatc ggaggcagca agctatatca
    22441 acagcacact ataagctgtc atactgagca aggcgaagaa tcaataaata cttcacctcg
    22501 ctcattacta ctaacaaact atacattgag catgatgaaa caacgaatag gagtatatat
    22561 atgccattgc gggggaaaca tctctgacta tgttgatgtg gaacagcttg gaaagttgat
    22621 ggcaaaagag gatggagtaa taatatcaaa ggatgtggtt tttgcctgct cagattctac
    22681 tcaaaaagat attgagaaag atattaaaga aatgaatctc gatgccattg tggttgcatc
    22741 gtgctcgcca aaacttcata cgcatacatt ccgcggggtt gcagagcgtg ctggaattaa
    22801 tccttacaac tacgttcagg taagcgtgcg cgagcaatgc tcgtgggctc attccgataa
    22861 accaatggat gcaaccataa aagcctacgg cctaattcgt gcaggcataa atagagtatc
    22921 acactccgag gctttggaaa tcatcgaaat taaggcacaa aaggctgttg ctgtaattgg
    22981 cgcaggagtg tcgggtttac gggctgcaat tgacctagct agaatgggca accaggttta
    23041 cttaattgag aaggaagctg aggttggcgg aaaagtatta acctctggca agctatttat
    23101 gactggagaa aatggtaaag aggttatcga ccggctatac caacgcataa aggaaatgaa
    23161 caatatcaac atattcacaa atgctaacct tgaaaatatt tcgggaagct ttggtaattt
    23221 tatgattgat gtgaatgttg ctggcgaatt gctcaagctc aacgttggct cagttctagt
    23281 aacaaccggt ttcgattttt atcaacccaa agagggtgaa tatggatatc agctatccga
    23341 caaggttata acacttcctc aatttaacaa acttatcaat tccaattctg ggaagcttac
    23401 ttacaatggc aaaaccatta aaactatagc ctacatttac tgcgttggaa accgccagag
    23461 caaaggtgaa aataagtact gctcacgtca atgctgcact gctgcaatct attcatcact
    23521 gaatgttcat gaaaaatttg aagggataaa ggcatttcac ctatatcggg atataagaac
    23581 ctacggtaaa caggagctac tctaccagga atcatcgaga aaaggagatg tttacataaa
    23641 atttgaggaa aaggaacccc ctgttattga agttaatgag gataaactct caataaaggt
    23701 taaggattac cttacttcaa agaaagagtt aatgctagat gccgatttag ttgttttggt
    23761 aaccggaatg gtgtcgcgaa gcgatagtag cgatatctcc ggaaagctaa aaatacccat
    23821 tggtaacgac aaattcttta acgaaatcca cccaaaacta aaaccggtag aaacagtaat
    23881 taagggtata tacattggtg gggcatgcca ggggccaaag aatatatcgg aatcggttca
    23941 atcggcactg tcgggtgcag caaaaatcaa cgcagctatt tgcaaaggaa gtatagagct
    24001 ggagccgatt gttgccagaa ttaactcaga aacttgcatt tggtgcaata aatgcagtga
    24061 ggtttgcgaa tttgatgcca ttaaaccaat tgaaagtaat ggcaaaatga tagcagaggt
    24121 aaatatctca tcctgtacag gatgcgggat atgtgcgcca gtatgcccta ccaacgctat
    24181 tgaagttgca catttcaccg ataacgaaat tgaatctatg attgatggct ttgcctcacc
    24241 ggtaaacatt ttaccaagag agcatgctaa ttcgaaaaca agttcctcca aggcacaaag
    24301 ccttgcagga taccctaaaa tatggagaac aatagccaat gcgctcaccg agaaacctct
    24361 taccataccg caaatagcgg aacaaattaa catcagcgta caagatgtaa cctggaatat
    24421 tatgacaatg aaccggtatg gatttatcga accagccgga cttgatgacg atgaagccta
    24481 ctacatgtat aaactcaaaa agtaattatc atgccgcaca taaatcctga ttttgctgac
    24541 gaaataaaaa aatacggagc cgatgatttt aatctttgtt ttaattgcgg caactgcacc
    24601 gctgtatgct ctctctccac aaatgacgaa tcgtttcctc gcgaaatgat tcgttacagc
    24661 acgcttgggc tggaggatga aataaaatca tcacttaaac cttggttgtg ctactactgt
    24721 ggtgagtgta gcaccgattg tccccgacag gccaacccgg gggaattgat gatgtcgctc
    24781 cgaagatggc ttactgccca gtacgattgg acaggccttt caggcttgct ctacaagcac
    24841 attggcgctt acattggagg tatgttagtt ttacttgccg gagtcatagg tttcaccaat
    24901 ttctcgggtt ttgataggga gcaagtaata cattacggcc acctcttcga gatgctcgcc
    24961 attgcaggag tttttgctgc tatacttcta cccaacataa tccacatgtg gtaccttaca
    25021 atagttaaac ccaaggtaaa accttccctt aaagtttatg cccagtcaac tggcgaccta
    25081 ttcgtgcaca tgtttaccca aaaacgcttt ctagggtgtg aggataccag agaaaaccga
    25141 acaagatgga ttttgcactt tatcctggtt ctgagctacc tctcccttct cttcaccacc
    25201 gtgtttctaa actggttctc tacaaccagc aaattcgtca tctggctggg gtactttgag
    25261 agtgctgcaa tttttattat taccaccgtt tttatgagta tgcgatttcg aaaggttgac
    25321 acaatgcaca agcattcaca accatcggat tggttctttg taatctggct cttcctaatg
    25381 ggaattactt cattcgcagt acggctgctt attgacctga acaacctgga cagccagttt
    25441 tggatgtaca cccttcacct taccgtcctt gcacaatggg cgttgattat agtccccttt
    25501 ggcaagtgga cacattttct ataccgttca tttgccatgt attttcaaaa aatactggag
    25561 cttaacagga ctgttaaaag gccataaaag caaacctcat caaaacaaca aaaagcccgc
    25621 tggtaatatt attctaaaaa atgctatatt gccataaatt taaaccactt cacccatgga
    25681 aagaaaacaa agaaaatatc aggaaaaggc acaccagaca ctcgatgaga ttttcgagag
    25741 tatagactcc cttgacgaga aaataaggca ggcaagcgac aaagccagca gcaccatgaa
    25801 ggaaaatata gaggagctaa aggcagaaag agaaaaactt aaggaaaagc ttaaggaatt
    25861 gaacgaggcc agtggcgagt catggaagga gataagaaat ggatttgagg aagcggctac
    25921 ctcgcttcgc gaatcattta tacgtgccaa aaacaaattt caggattcag aacaatagtt
    25981 tcatcttatc gaaggggcat gtaaagcaga acgaaaaaat ttccttgcag ggctattcag
    26041 caattctaat gtgatgatgg agttaagaaa atggctgctg gtatttagta ctgtgtttag
    26101 tgctatagtt tttgctaaat cgcaggaagc tgacagtagc tccctaaccc ttctttttgt
    26161 gggtgatgta atggggcatt cgccccaaat ttcatcagcc cttaatcctt taaccggtga
    26221 atatgagtat gatagcgttt ttaccagaat gaagggcgta ttctcaatgg ccgacttcac
    26281 cattgccaac cttgaagtta ccctgggcgg agagccttat acagggtatc cgcaattcag
    26341 ctcacccgat gctcttgtag atggtctaat aaatgctggg gtaaacgtac tggttactgc
    26401 taataaccat tccgcagaca ggggcgctaa gggaatacaa cgaacagttg aaaccctcga
    26461 ccgcaaaggg ataattcaca ccggaacctt ttccgattca tccgacaggt tgaagaataa
    26521 ccctttaatt ctcgagaaaa atgggatgcg tattgccctg ctgaactaca cctatggaac
    26581 caacggactg cccgttccgg caccctgtat tgtcaataag atagacactt tggtaataat
    26641 aagggatatt gctatgacaa ggttgatgaa tgccgatgag atagtagtgt tcatccactg
    26701 gggaaacgaa tatgagcggt atccatcggc tgcacaagta gcaatggcta attttctaaa
    26761 ccgccagggg gtaagaatag taattggctc tcatccccat gtagttcaac ccatggaagc
    26821 caccttagat accgatagca caaccggcag aatagttgtt tactccctag gaaactttgt
    26881 gtcgaaccaa cgaagacgct actgtgatgg gggagcgctt gcagccgtaa agctggttaa
    26941 gaaatctaca ggtaaatcag aaattactgc tgcaggtttt cttcccgtat gggttcatac
    27001 gccctcaacc gcctcccaca aaacgtttca ggttcttcca gtttcgatct acgaaacagc
    27061 tccaaaaggc tacttttctg ccgatgagct ggcacagctc acccaatttg ccaccgatac
    27121 tcgtgctctg atgcacgaaa aaaatctgaa cttccccgag cttatctttt acaacggcag
    27181 ctggattata ccagacaata gagacaaatt acccatcact aggttgacca ccgggcaatc
    27241 tcatttttag cataaatcaa agcgaataag attttcttga cgaagcattc ccttcgctgg
    27301 taatattata tgctaccaac ctgcatgtat ttataaaaaa aacctgccac acgagctacc
    27361 ggaaatgaat gcttaaaaat ttaaaatttt atacctgcgt ttatacctac cccttttata
    27421 gctatgtttg caggattttt aattaattat taacatcaac ttaacgctaa gttaatgaaa
    27481 caacgtttct cattcctgat aaggtttttc cttttctggc tgatttactt catagtcttt
    27541 agagcagtat ttttggttta ccagcttccg ttaacgcggg gtataagcat gggtgatgca
    27601 ctaatgattt tcacgcgtgg aatatggatg gacacttcgc tggcaggata catactgctg
    27661 ctggcctcgc tgttcatggc attactatat tttgttcatg gacgttttat tcgaacggtt
    27721 tacacaacct ataccattat tttgctggtt ctattttcgg ttataaccat ttcggacctg
    27781 gagctttacc gtaactgggg tttcagaatt gatgccactc ccctactcta cctcaaaact
    27841 cctaaagatg ccatggcctc tttaaagggt tgggtaattt tagtgctagc cattgcagcg
    27901 gcaattgcgg ttatctcatc tatgtttttt taccggaaat gggttgccaa acccctcaac
    27961 cacattgaaa aggggaaatg gtggtatttc ccgattttca cattcatagc cgcctgcatg
    28021 attattccta ttcgcggggg attcggaatt gcaccaatga atcctggcaa ggtttacttt
    28081 agccagaacg tttactgcaa ccatgcagca ttaaacccag tatggaacat gatgtactct
    28141 gtttccaaat cgggaaaaat gagcaagcaa tacccgacgg agattaagcc aagcgaagca
    28201 aaacaacttt tcaccagcat gatggaatcc gactccacca actacgttct ctcctctaac
    28261 aggcctaata ttgtggttat cctgctcgaa agctttagtg ctaaattaat tgagcccctc
    28321 ggaggaaaaa gtggcgtaac tccatgtttt aacaggcttt cagaacaagg tatattgttt
    28381 acagatgcct ttgcaagcgg tgataggagc gacaaaggaa ttattgcggt tctttcaggc
    28441 tttccggcgc aacccacaca atcaattatt aagtatccaa ccaagtcgcg taagctaccc
    28501 actataagcg gtgtgctggc cgaaaacgga tacaacaaca ctttctacta tggaggcgac
    28561 cccgattttg ccaatatccg ttcgttccta taccatgcaa agtttaacag aattgtaacc
    28621 caagacgaat tccctaaaaa ataccgcaac tccaagtggg gtgttcacga tgagtatgtt
    28681 ttcaactacc tgcttaccga tctcgatagc gccagggctc ctttctttaa attctttttt
    28741 acgctaagca gccatgagcc ctttgaaatt ccggccaaac caaaatttaa aggtagtgat
    28801 gaggttaacc agtacctaag ctcggcatac tataccgata gctgcctggg aaattttttc
    28861 aatattgccc agaaaaagaa ttggtacaaa aacaccattt tcatactcat agcagatcat
    28921 ggccataggc acctaggaaa tcatcccaac tatgccgttg aaaaattcca tattcccatg
    28981 ctgtggatag gaggcgctct tagcgttaat ccaacacgaa ttgatgaaac tgtttcgcag
    29041 gttgaccttg cctcaacgct cctcaaccag ctcaacctca gcgattcagc ttttactttt
    29101 tccaagaata tccttagcag ccactttact ccttttgcat actatgcctt taatgacggc
    29161 tttggtttta agaataatac cgatacggtc atctatgacc atgcaggtaa gaagtacatc
    29221 aagatgagcc gagcaaaccc caaacaggct gctcaggaag catgggcatt tttttccact
    29281 taccaagaca cctttttagg atactagtgt gcttgtcgcc ttatcaaatt aaaacgggga
    29341 ggttccttgc ctccccattt ttttacttgt taaggtttgc gttgccatac ttaatcctga
    29401 tgtacacttc agcatttgtt ctgctatttg ttcctaccag gcccgatacc tgaattgtgg
    29461 tgttgtcttc aattcggttg acagatgatt tatcggcgct gaaatttata ctaccatagg
    29521 ctgcttcagc gtcaattctg taggaaacat tagggttgat accagccttc aagccggtgt
    29581 aggccccctc aaaccgaatg ctctgaaagc tatcagacac atctattatt ttaacgtttc
    29641 catacctggt ggttaggtta aactttttgc ccaagttgga aatatcgaaa tcggtataac
    29701 cagactctcc ttccaaattc ataaccgaac caacctcata cctatcatat ttagattcaa
    29761 gaacaagcga gtttaccctt cccaagtaaa ccttggagta ctttgaaaga actaccattg
    29821 cctgtgctac ttcaatgtta aggttgctgt acttcatttc aacctttaac cagtttgcct
    29881 cggaaatggc aacatcacca tatccaacgg taatggagct aagaggttgc gaatcgccca
    29941 gccctagctt gttagcctta aaattgccgt acatcaaatc aactgtaatc ctggcattta
    30001 gctcgttcac aaatatattc ccatacttat tcaacagctc cacctcgata tttttcggca
    30061 taaatatctt atagttaatg ctaagctcgg tgttagagaa agatgagaac agtctaggag
    30121 atgactttcg aatatcctca ttaaaccggg taatagccga aaccaggttt tccgtttcag
    30181 aaaactcaac attaatagca tcaagcatag atttagccct ttcggcgttt gcgttttcaa
    30241 ctttcacatc tacatgaatg gctatagcgc ttttatccca attctcaatg ctaatttggc
    30301 catacttgtt ttcaacgtaa agtttagatg caggcgaaac cttaaactcc ctgtcgtatt
    30361 gtttagaaag ctcgagggat gcggaatatc cgcttaagct gctcagtaat aagaatagag
    30421 ttacagaaat tctgagaaat gctttcatgg ttttttatct attaagttaa attattaaca
    30481 tggcaaaaag tgctgctact tccccttatc aagtgttcct tcaatttttt gtagaacttc
    30541 cagccttgac tggtagtttt cgtacattgc tccaagcacc atgtcgctgc ggggattctt
    30601 tggtagttct gctttaaggc gttgaccctc cctatcaaac tcctcaagga gctgcttggc
    30661 ttcaggtttt acagaaggat ccgttgatga ggcaagtttt tcaatcgccc tgtatttttg
    30721 gagcagctgg ctccggtagt aggcttctac ttccatgaac tgtgcagttg accctttaga
    30781 aaaatgccta gaaagctctt cagaaaagtg gttgccatac attaggctca atgcagcggt
    30841 tagtgcaaga atgatggaaa ccgaagcggc agctagtgca aaagttttaa tggttgtatg
    30901 gtttttttgg gcatgaattc gggacttaaa ccgctgctca tgcccatcgg gtaggtccac
    30961 atcgtcaagt tgacctttgt tttcggcaaa aaatttctca agcttatcca ttgtattgcg
    31021 tgttagttcc tttttaaaag ttcaaccagc ttttgacggc cacgcgtgta ttgcgatctt
    31081 accgatgact cattaatgtc aagcatttcg gcaatttcag ggtaatccag ctcttcaact
    31141 aagcgcaggg tcagcaccac cctatatccc tcgggcagct gctgcatagc gagcttaacc
    31201 tgctcaaccg attcgggggt ataatcgtcg ctactgtcgg tatgctcggc taagccaggc
    31261 aaatcgtcga acgaaaccag ctgcaccctt ctttttttaa gatggtcgag acaggtgttt
    31321 atcacgattc gcttaagcca agcgccaaaa gtaacctcgc ccctgaaagt actaatcctg
    31381 tcgaatgctt taaagaacgc ttcctgcatg gcatcttcgg cttccatcct atcgggaatg
    31441 atgcggcagc ttacattata catcgctctg gagtacattg aataaagttc aaactgcgca
    31501 cgactatcgc ctcggcaaca cttttttaca agctcggagc taatatccat tcctaatcag
    31561 gctaaaaacc aaatacaacc gaaagacttt cccatggatg cgtttgctgc atgggcaggg
    31621 tattttttca aatttaggtt taatttttct ataaaaatac ccaagcatgt ttgtgtcaga
    31681 acagtaataa aacaaacaaa aagcctgatt gcctataagt tggcaaatca ggcttttagc
    31741 aattgggtta tgtagctcta cactatgcca gaaagctcaa aagaataccc gcggcaacgg
    31801 cgctaccaat aactccggcc acattgggcc ccatggcgtg catcagcagg tggttggttt
    31861 tatcgtactc caacccaata acctgcgaaa ctctagcgct atcaggaaca gctgaaaccc
    31921 cggcatttcc gattaacggg ttaatttttt gcccctcctt caggaaaagg ttgataattt
    31981 taacaaacat taccccacca aaagtagcca ctataaacga aatggctcca attataaaaa
    32041 tttcgagtga ttttgcagtt aagaaggtgg ttgcctgggt agaagctcct actgttagcc
    32101 caattaggat ggtaacaata tcaataaggg gtcctctggc cgtttcggca agccttttgg
    32161 ttactgtgct ttccttaagc aggttcccaa agaatagcat tcccaaaagc ggcagccccg
    32221 aaggaactat gaaggcagtg agaagaattc ccacaattgg gaacaagatt ttctcaagct
    32281 tggtaacctg tctcgggggt ttcatacgaa taacgcgctc cttcctggtg gtaagcaacc
    32341 tcattatagg aggttgaata acaggcacca gagccatgta ggagtatgct gaaatggcaa
    32401 tagcacccag tagctccggc gcaagttttg atgaaaggaa aatggctgta ggtccgtcag
    32461 caccaccgat gattccaatt gcaccggctt cctgtggaga aaatcccaac accaaggcaa
    32521 gaatataggc accaaaaata cccagctgtg ctgcggcacc aatcagcata agcttagggt
    32581 ttgatatgag cgcgctaaag tcggtcatag cgccaatgcc cagaaaaatg agtggcgggt
    32641 aaattccatt tagaacgcca aagtacaggt agttaagcac gcttccactt tcataaacac
    32701 caatctggta gccgggtgaa aaagggatat tacccacaat gattccaaag ccaataggga
    32761 caagcagcat gggttcaaat tcctttgaaa tggccaggta aataaagaat aaacccacca
    32821 aaatcattac cccatgtcca attgtaaagt tggcaaaacc tgtaaatgag agaaactggt
    32881 tcaggttttc cactaagaat gaaaaaaatc ctccgctttg ctccatggct atccgataat
    32941 tattagaacc tctccctcca gcacggtatc gccctttcta acctttacct cggttactct
    33001 tccggcctta tcggagtcaa tattgttctc cattttcatg gcttcaagaa caataaggcg
    33061 ctgccccacc ttaacctcgt cgccgggttt aaccagcaca tccagcacca cgcccgggag
    33121 cggagacttt accatacccg tagaaggtga atcgggacta accgttttta ctactgatgg
    33181 atgtgagtcg gtagcaggta ctgcttcttg cctgattagc tttggcgttt tagtttgcct
    33241 taactccctt tccatttcaa ccctgtaggg tgttccgtta acctctacat cggcaagatt
    33301 gccatcaaca ttggcaatgt gaacgctgta ctcattgccg tttattttga atttaaaatc
    33361 cttcattctc gtctgacatt aagttttact ttttcttctg gtgtgttcta ctgtttgttg
    33421 tgtacgcggt gggtaactac acggtttggc tgctgcgtta agctgtatag ttttgaactc
    33481 cagggagagt aatgtctcga aacccgctga atggtgatta tttccgattc ctgatcgtgc
    33541 tgttcggttt cataaaggta aagagcagca gaaactgcag caagaatctc tcccgatagt
    33601 tcagccacct gcacctcctg ggttttacca acatccctac tttccctttt tcctacccta
    33661 agaggttttt gcatcatcat tcctaaaaac ctaaatattc ggtaaagaat aaaaagggca
    33721 gtaaacacca ccagcatagc aatgcaggtc atcataattc cgaaggggtc aaactggaca
    33781 aatttctcgc ccgatgtttc ctcgtgtgta aaaaggttgt tggtggcatt gggcgtagta
    33841 aaactggtta tttgccaagc gctagtcccg tcgggtatcc tgcaatacga ggtattcggc
    33901 aacgagaggg gaacggtaac gctgtctata aggctccttc catcggagct gtaaagggca
    33961 acaaacctgt gggtgctgtc gagtgtaaaa ttgagatgga gcacaccatg atggcttttc
    34021 ccgttggcaa aaaatacaag aaactgctgt tggggaattc tggttaccgg atcacccttg
    34081 gggataaggt atttttttgg ctgggtaggg tcgtcggtaa ggtagcaacc accaatgtta
    34141 acggtgttat agcccaagtt cataatttca atccatgaac ttctctggcc atagctatca
    34201 atgtagttct cggtgttaaa gagcattacc tcgttaatgc gcaaatcgta aacactctgc
    34261 gcggtagatg aaacagctgc tcccatccac agtagggctg caagtatgcc atgtataaac
    34321 ttcttcatca tgccaaatta ttatttcgaa attctacaac ggcaggttag aatgcttctt
    34381 gggcggatta accacctttt ttgtagccag cgactgtagt gccctaataa ctcggaaccg
    34441 ggtgttgcgc ggttcaatta catcatcaat atatccgtat tttgcggcaa cgtagggatt
    34501 tgcaaacttg ttcttatact cttcctcctt gcgtaacaag aaatcatctt tggctttctg
    34561 gtcttccaac gtttccagct ccttgctgta aagaatttca atggcaccct tggggcccat
    34621 aacggcaatt tcggcggttg gccaggagta gttaatgtcg cctctaaggt gcttggagct
    34681 cataacatca taggcaccac cataggcttt ccgaagaatg atggtaattt tgggaacagt
    34741 agcctcaccg taggcatata gcagctttgc cccatgaata atgatgccgt tgtactcctg
    34801 tcctgttcct gggagaaaac ccggcacatc caccagcgtt accagcggaa tattgaaaga
    34861 gtcgcaaaag cgaacaaacc ttgcagcctt tcttgaggaa tttatatcaa gcactcctgc
    34921 caggtactta ggctggttgg ccacaatacc caccgattga ccgttaaatc gggcaaaaca
    34981 agtaataatg ttgtgggcat aatcgcgctg aacctcaaga aactcgccgt agtcaacaat
    35041 tgctgtaata acatccttaa cgtcgtaagg cttacttggg ttatcgggga taatatcgtt
    35101 gagggaatcc tcaaggcggt cgattggatc gtcgcagggc attattggtg cttcctccag
    35161 gttattttga ggcaaatagc tcagcagctt cctgataagc attatgccct cttcctcgtt
    35221 ttcggcaaca aagtgagcca ctcccgattt ggacgagtga acgccggcac caccaagttc
    35281 ctctgaagtt acggtttcgc cggttaccgt tttaactacc ttggggccag ttacaaacat
    35341 atagctggtg tttcgcgaca taacgatgaa atcggttaat gccggagaat agacagcacc
    35401 cccagcgcag gggccaaata ttgcagaaat ttgaggaaca accccagaag ccaggatgtt
    35461 tcgctcgaat atttcggcat accctcccag gctggttacc ccctcctgaa tacgggctcc
    35521 accgctatcg tttattccaa tgaccggcgc accaaccttc atggccatgt ccatcacctt
    35581 gcaaatcttg gcggcaaaca tttctgaaag cgagccacca aacaccgtaa agtcctgtga
    35641 aaaaacataa accaagcgtc cgtcaatagt tccatatcct gtgattacgc catccgatag
    35701 gtaggtttcc ttatccaggc caaaatcagt acagcggtgg gtaacaaaca tgtcgaactc
    35761 ctcaaagctt ccctcgtcga gaagcatctc tatccgttcg cgggcagtat attttccttt
    35821 cttatgctgg gattcaatac gtcgggcacc tccacctagc ttagcttttt cgcgcagctc
    35881 aataagctct tttatttttt gctcctgaat gctcatatcg ttatttttaa ggataaacta
    35941 tttctgattt ctatcttcac agaattcggt cagcactcca aaggtggact ttggatgcag
    36001 gaaggctatc tccaaccctt ccgcaccact gcgtggagtt ttatcaatta aaactatgcc
    36061 attctcctgc atacgttgca gctcctcgtt tatacccttt acagcaaagg ctatatggtg
    36121 tattccctca cctttttttt caataaattt gccaatagta ccttcgggat ccgttgtttc
    36181 caggagttca attttggttt gccctacctt gaagaaggct gtcttaacct tctggtcttt
    36241 tacctcctca atgctgtagc acttcactcc caataaattt tcatagtagg ggattgcatc
    36301 atttaggctc ttaacggcaa tgccgatatg ttcaatatgg gttacgctca tgtgagtaaa
    36361 aatttaggtt aatacttttg tggcacaaag ttaccctgaa atggtttgat aactaggaaa
    36421 aagggcaacg aaattgcctg tttttcaaca ttgttaaaca acatgttttg ttgttcagct
    36481 acaggttgtt ctattatttt ggaatattct gcaaaaatca acatggcatt acgaaacgcc
    36541 cttgtaagga aacctgcaaa acacgcttga acaaccgggc cttttgctag cttggtgaag
    36601 ccaaaacatt gggtgttccg tcccctcctc cccgatgctt aaaagttatg agctaaagta
    36661 cttgtaatca gaaccctatt ttttttaaat ggttgaatga gctaatcaac cgttaaagct
    36721 gttttgaaat ccactttata aatttataat ttagcacgtg caaaaaagca cgtttggcct
    36781 tgacggtgaa tggtaagaat agacaaaaat cacagcggta ccaatcgttt cgacggttag
    36841 tattatatgc catcgccaca ttctttatta caaccgtcat atcggtaata gctttttctt
    36901 ttatcaatcc aaaccttacc atgctaatgg tagccaaacg agttcagccg ctaaatgacg
    36961 gcggatctaa tcggattaat cacaagtggg taagcattga taaaatatct ccccagctag
    37021 ttttggctgt gtgtgctgct gaggataata agtttatgga acacaacggg ttcgactggg
    37081 agtccattca aaaagccaag gagcataata aaaaggggaa aaggctaaga ggcgctagca
    37141 ccataaccca gcaaaccgta aagaatgtat ttctatggcc gcgccgttca tgggtaagaa
    37201 agggatttga ggcctacttt acatttctgg tagaagtttt ttggagcaag gagcgaatta
    37261 tggaggttta cctcaatgta gccgaattgg gaagaggcat atatggagtg gagtctgctt
    37321 cggaatgcta ctatggaaaa cccgcttcaa aaattagcac ctatgaggct gccatgctgg
    37381 ctaccgtgct tcccaaccca gcaaagcgta atccggctaa gcctagcagc tacatgtaca
    37441 ggtaccagcg caccattctt cgaaacatgt acaacctggg aagaatagac cttaaaggag
    37501 atacaagtag taaaagcaaa aagaaaaact aaaaaagtga gtaaattgaa acggcatctg
    37561 cctatactgt ttttaataat actcggtagt ttaggccatt caccgcttta tggccagcta
    37621 aacaaggcct actactacta caaaagcgaa gagttgattt cgcaagggcg ctacagcgaa
    37681 tcgctaccgg ttttgaacac ccttatcgga atagactcct cccttttcga gggctggttt
    37741 cttagagggg tggcaaagta caacctgggc gacataaggg gtgcaatctc cgacttcgac
    37801 aagtcgctaa aggctaaccc gctatctgct caatcatacc actacaaagc aattgcctta
    37861 agtcaggtat cagaatatga caaggcgttg aaggagatta gctccgcact ggagcttaga
    37921 cctaactccc ccgattttct cttcacactc ggtgtgctcc aattccaaac ccagaactac
    37981 cctaaggcaa taggaacctt cacacgggta attcagcttg aaagttccaa tgccgacgca
    38041 tggtataacc gcgccactgc caaactgctc atagccgata ctctgggtgc tataagcgac
    38101 tacagcacaa ccattaatgt gaacccattc catgctgcag catacgcccg caggggagct
    38161 gtaaacgccg aacttgatag aaaccacctt gctttagccg atttaaacag agccattgag
    38221 ctcgacagcc ttaaggctga cttttacttt acccgggcca ttataaacta taactttaaa
    38281 aaatatcaaa actctattga tgaccttaca aaagccctac gattaaatcc aaactaccaa
    38341 atagcgcttt ttaacagggc aattattaac taccaggcag gaaatacaga cccagcaatt
    38401 gacgacctta caaggctaat ggcaataaat tcccaaaatg ttctggtgcc atactacaga
    38461 ggttctatat acctggaaaa gggtgaagca acacggtcta tcgacgattt taccaaggcc
    38521 ataagccttt ttcccgactt cgccaatgcc tacctcctcc gctccgatgc aaaaagacag
    38581 ctgggtaacc taccgggagc cgaggaggat ttcctgaaag gccaagctct agctaataaa
    38641 ttccatggaa agggacaggc cgaaatttct tcgttgctcg actcgagcgg aaaactcaac
    38701 aagctgattt cgcttgaaag cgattttaac ctggcttcta cattaagcat cgaatcaaac
    38761 agaattcgca ttgcctctgc attcatgcca atgataaggc ttcattttaa tcaacccgat
    38821 aaaccagcaa caaataacaa tcttgcatcc gtttaccttg attctattaa tgcaacactt
    38881 cccataaact acaagttaat ctttgggttt gctcccagca actcaacaga aaagctttcc
    38941 atagaaagcg ccgattccac tatagccaac tttccggcat tatgcctggt taagggtata
    39001 tttttagcag aaaatggcaa gtttgctagc gccactaacg aatttaaaaa ggcctttgag
    39061 caaaatagca gtaacccgat ttacctcttc tcctacatca tgaataatgt tgaaatggca
    39121 gaattcattg aaaattttaa cacctcaaca gaaatctccg gcattgctat gagctcttca
    39181 aaaaatacag gaataacaag cattacagca tataatgatg ctatagtcga actaaaaggt
    39241 ttatatgaag tgttccctaa aaatggtctt attgcttaca acctggggaa tctaagtatt
    39301 atgacgaacg actatgaaag cgccattagc tggttctcgc tggcagtaag agaaaatcca
    39361 cttctgagct gtgcatggtt taacaaaggg cttagcctac ttattaccgg caacaaggat
    39421 gagggctgca ataacttagg taaagccgga gagctaggtg ttgaggaggc ctacatggca
    39481 atcaaaaaat tttgcaccag gtaaacagct ttatataaaa ttgaaagcca agcccgaatt
    39541 agcggggtgc cggctaaagc cactctcaaa aaagttagct gctcttctct agcagcattg
    39601 acattaatta ccaactacaa taaaaattga taaatattag ctggtaaata atatcatggt
    39661 aaaattttta catttgctgt tacaataata acattaatct tgatagatca ttttggctat
    39721 caggcaaata gtataacatg ggaaaactat ttgacaggtt gaacagcgat gtcaggttta
    39781 ttgagggaat caacggctgc ataaactgtg gaacctgcac tgcaatctgc ccggcggcag
    39841 aagtttacga ctatgaaccc cgaagtattg ccaatctggt tcaaacccac gacgacgaag
    39901 tgattgagaa cttgctgaaa agcgatacaa tatggtactg cggggagtgc atgagctgca
    39961 aaaccaggtg cccccgaaat aatgccccag gactaataat tcaggcactc cgagggcttt
    40021 ccatcgaaac cggccatttt gttgaatccg aaaaagggag gcagcagctc tcgcttaaac
    40081 gaatgcttgg caactccatt ctcgaaacag gctactgcgt ccactttgac cataccgagc
    40141 taagcatgtt tcctgagcta ggccctgttt ggcaatgggt tagggacaac cgcgaaaccg
    40201 tgttaaaaaa agtaggcgcc aactataaag gcacaggccc cggggcaata aggaaaattt
    40261 cacaggaaag tctcgatgag ctggaaagga tttttgaggt aaccggaggg ttggaatggt
    40321 ttaagaaaat agaggaggat tcctcggaaa tggccgataa gcttagtctc gaaatcggca
    40381 acgagactag caaggagtca ccatacttcc gaaaggtata cagacacaac gatcctgaga
    40441 agcacacacg taagtagttt aaataaagct agctgcctgc acaactatac ggtattttaa
    40501 aaaatgggag caatcctcct accattttca caaacgtaaa gaatatctga gctaaacata
    40561 acaggaacca tgaaaggcaa cgaggtaaaa aagaaaattt ggagcgaata ccagaaggaa
    40621 attgcagatg ataagtttta cttcgtccgc agctgcatac gtcaaacatt tttcccgggc
    40681 agcgatacag tttacctcaa aatactgcgg gatgtgctgg gtaaggacgt ttacgaggat
    40741 gcaaaccaca ccacctgcac cggaattggc taccactgcg aggtggttcc ttttccaacc
    40801 atccaaacag ttgtggcgcg caacttttcc ctgatgcgtg agaatggtta ccaaaactac
    40861 aacccatcct gtgtaacctc atttggtata tacaccgaaa tacttgaaac atggcaccat
    40921 ttccccgagg aaaaggaaaa gactcgtgaa taccttaaaa aggcaaccgg aagggaattt
    40981 gaagaacccg ccaacctggt ccatactagc gatgtgctct ataagtttcg taacgaaata
    41041 gctgcaaaag ctaaatacag actggtggat agggaaaccg gaaaaccgct gaaggtagtt
    41101 gaccacattg gctgccatta tgccaaaatg tttcccagca aagggattgg cggtgctgaa
    41161 tacccccagg tgctggcagg tctggtagaa gcttttggag gcgaagtagt tgactatcct
    41221 gagcgtaggc actgctgtgg gtttggcttc cgccagtact ttgttcaggc caacagggga
    41281 tactcaatag ccgcttcaaa aaagaaattt gaatccatgg aacccttcaa gcccgatttc
    41341 atcattacta attgtccggg ttgtaactat tttatggaca gatggcagta tgttctggcc
    41401 gaagctgaag ggaaaaccta tggacaagac ggttacggga tacccgttct ctcatacgag
    41461 gaggttgccg gagtagttct gggattcgat ccatgggaga taggattgca gatgcaccaa
    41521 accgacgtag agcccctact taaaaaaatt ggggttgaat acgaccccga caagaagttt
    41581 aaaggtgtaa ataatgaaac ccttagcttg cccgataaac caaataatat taaatttatc
    41641 gtttaaaact aaggcagaag ggatgcacag ctgtattgca aatcatacgt cagcagcaaa
    41701 aaactgtgga attgtaacca tctcttagga aacaaatagt aaaaccagta atgctgtaat
    41761 gataggcaaa gtaatgtagt caaactagtt aacgatgtta aaaaaagtag ctgtcatagg
    41821 tggaggtgtt gctgggctgg aggctgccag gcaactagca gcgttgggtt accaaacaac
    41881 cgtatatgag aaaaatgatc acgccggcgg tcatacctca gagtgggaca ggttattccc
    41941 aaacagaaga cccgctgttg aggtggttga cacgctcaaa tcccagctta acggaaatcc
    42001 ggccatcgtc ctttctacac ccataaccga actgctaccc aacggggata aatttaccct
    42061 tataaccagt aataaatctt tcgaagccga tgctgtgctg attgccacgg gttttgaact
    42121 ttttcaagcc gaacgcaaag aagagtatgg ttacggaata tatgacaacg taattacctc
    42181 ggccgagcta gaaaagatgt tcaccgaaca tggagcgccc ctgatgagaa atggcgaaaa
    42241 acccaaacga gttgcattcg tccactgcgt gggtagccgc gatgaaaagg tgaaccatct
    42301 ttactgttca aaagtttgct gcgtaacagc agttaagcag gcaatcgaag caaaagaggc
    42361 tatcccagag gctgaaatat tctgtctcta catggacctc cgaatgtttg ggcctggcta
    42421 tgaggagctt tacaaggaag cacagcaaaa gggaataaac ttcatccggg gcaggctatc
    42481 ggaggcaagc gagctggatg gtggaagatt gcttattaag actgaggaca ccctggcttc
    42541 ccgtcccctt aaaatgtcgg tagatatgct cgttttgatg gtgggcatga caccagccga
    42601 aggcacttcc aaacttatat ataaccttgg gcttaaacca aatgccgatg ggtttatcgg
    42661 ggtaagcgac tatcacctta aaccttccga aaccggcatt agaggtgtat atactgccgg
    42721 tgcatgttcg ggacctaaca acatcaccga ctcgataaac tctgcccgtt cggcagtagt
    42781 tgaaattcat aggtatttaa gcgtgaagga accatgattg attttggcta cgcacccctt
    42841 aaaataaaca ttcacgattt ggacaaggct gatctttccc taatggactg gattacagct
    42901 cgagacgaat cgttccgcct ctgcattgcc tgcggaagct gcacggcaac ctgcactgcc
    42961 ggaaatttta ccaacttcag ctttagggaa ctttgccaca gcattcgtag aggagaagtt
    43021 aaaaaggctg tgagggaatc ggaaaagtgc atgctttgcg gaaagtgcac cttggtttgt
    43081 cctcgcaacg tgaatactcg taacataata aagctggttc gaaaagccaa ccaaaatttc
    43141 agggtatgaa ggaacatcca tttgtaatcc cgttcaccat agggctaact tttgttatcc
    43201 tctatacggt tgtcatgtgg accatttggg tagtacgcct tacccggcgt gagaaaaaaa
    43261 ttatatggaa aagactgcta acagttaaaa cgctaagagg aatatgggag accataagag
    43321 agggtttgtt tcatcgaaat atctttaaga aaaatgttat tcttggcttt atgcacatga
    43381 gcctcgcctt tggatggttc atgcttattg tgctgggcaa cttggaagcc cgactttacc
    43441 cccacgggct aatcaaccct ccctactacc ctattttttt tgagtttttc cgccacgacc
    43501 tttccccgct gagggaatat gaggagattt tcacctttct aatggacttt ttcctgcttc
    43561 tggtcatttc cggaattggc attgcctttt ttaaaaggta taaaagtaag gtgatgggga
    43621 tgcaccgaac taccaaacac attctcatcg accgaatagc ccttacctcc ctttggctga
    43681 ttttcccttt cagaatactg gctgaaagcg ttactgccgg attgtataac accggccatt
    43741 tcttcacaaa tttcctcggc caacaggtta tctacggtct tccgctcgac cagctatact
    43801 acccctcatg gtgggcatac tccattgcgt tggggtcgtt cttcgttgcc ctaccctttt
    43861 ctaggtacat gcacattcca acagaaattt tgctgatttt tcttcgtaaa tatggtgtaa
    43921 gggaaaagga tagccactca tcctacacca acgttgagct aaacgcttgc tccaggtgcg
    43981 gtatctgcat agactcctgc caactaagca ccgatttggg aatttctgac acccaaccaa
    44041 cctacttcat tagggatctt agatacagca agccaagcga gaggcttaga gaaacctgta
    44101 tgctttgtat gagatgtctt aaagcatgcc cggtaggaat tgaaatcaca caaataagaa
    44161 agaacgaacg aagcgagcac cagcttttta gcccaaccag ctatagctac ctgaacgggt
    44221 acgcctaccc caaagctaag gttgcatttt ttgcagggtg catggggcat ttaaccccca
    44281 acataattaa atcaacgctt ctccttctcg acgctgccgg gatcgattac acctttatcg
    44341 accgaaacgg aagcatttgc tgtggtaggc cgttgcagct taacggagag gttgatgcgg
    44401 caaagcagct tattgataaa aataaccggc ttatttacga aacaggcgcc aactgcttgg
    44461 taaccacctg ccccatttgc gccagcgagt ttaagaagaa ctaccacatg agcattccca
    44521 tataccacca tagccagtac ctctgggagc ttaccaacca aggcaggctg gtttttaacc
    44581 aatccaacct gaatgttgct taccacgatc cgtgtgagct aagccgtggt cttggtgttg
    44641 ttgacgagcc acgcaagctc ctaaagagcg ctgtggttct aaccgaaaac agcaaccaaa
    44701 ccaaacaaaa ttccctatgc tgcgggggaa gccttgcaat taccaacacc tctgcatttc
    44761 aaaagtcaac aattgcggca tcaaccgttt tgcaactagc caataacact accgacctga
    44821 tagccacagc atgcccactt tgcaagaaaa cccttgctgc cgcccaaaat gatgtccccg
    44881 tccaggatat atccgaaatt atggtgaaag cgcttatgga cagccgtaag gcaagccttt
    44941 cagaggctaa agttaagatg gctagcccca aacttgaggt agtcgaggaa tacaagtagg
    45001 tgcggtaagt actcatggca ttctgccttt caaaactttc aagccggtat ttattacttt
    45061 ccagcaacta atgattagga ttacacaacc ttaatcaaat tttttttaag acatgtaatc
    45121 aatttcaaca tccttcttcg ttaaaggact agaccataag tgtaataaat ccagaaggaa
    45181 ttcatttttt atcctaagtt ctaattttat ttgccggatt ttcctatttt tacacatata
    45241 ctattactat attggtttca atcatgttct aacaaatact atcctatgaa gaagtttcta
    45301 ctagctagcg ttgttacact tggcagctca tgggcattgc tggcccaacc cgagcgaatg
    45361 ccttttggcg aaaatatgca gtattcctca gggaaacatt tatttaatgc tgagagtagc
    45421 ggagcaaagg gaattaatga aatgtacttc tccgacgatt tcgagggttc ctcatacatt
    45481 ttgtcggata aatgggaaat tctgcgtagc agcactctgg attatagcag caccatatca
    45541 gctgctaatc caatgtggtt ccactgcacc ccggaaagct tcaacggcaa tggcgccacc
    45601 tacattaaat ttggaagagg ttctgctgct atttcctaca ctgctcccga ttttacctgg
    45661 cttgtttgta aggatactct tcctatccca caaaacggtg aggcttacct aaaattttgg
    45721 ctctggtact ccagcaattt tgaccagggt tactccaccc atttttacat acttgcatac
    45781 aataccgatg atcacgccat tgacactctt aactattggg gtgagtatga ttcaaccagc
    45841 atttcaaatc aatatgcacg ccgaataaca gtaccacttt cactgcttgc aggcaaaaat
    45901 atcaggcttg cattcgttta tgagaatagg aataacgaaa ataaaggcat tcagctagct
    45961 gttgacgagg ttgaaattac caatagtaac agtccggata tttctttgaa ggcagtccca
    46021 ttcacctact cccagatacc ctactttacc ttcgattcac tttttttcaa cctctatgct
    46081 gacatctaca ataccggagc agcaattagc gacaccattg aaattgttgc cacatcggag
    46141 caacttcctg atattaatgc ttttgccact atcaccgata ccttaaacga aggtgagcaa
    46201 cgtttagcta agctcaacgg acaggctaaa attactgaaa atacaaacta caccataggt
    46261 ataacctcct caacagatca ggatagcata accgccaaca actcggacaa tatcgttttt
    46321 tcagtatccc agtcggttta tgctacagac catggtgcaa caggtggttt tacactcgga
    46381 gagaatcaac cctttggcaa cattttcaag gttaacaagc cgtcatttat caagggtatt
    46441 caggtgggct ggtcgcctat cagcactatt cccgaagcag aataccccat tccattctgg
    46501 gtagcagttt accagcttga ccccctggat agctcctgtt atttccctgt aaaaacagga
    46561 tatttcgaaa aacagtactc ccaggtgggc gcttcaagcg actacccctt tgacgaacca
    46621 atctactgct taccaggatt ttcctacttt gttactgtaa cccagttaag tagcgctagt
    46681 cttggtgtgg ggtgcgatgg caacagctac ggctcgtttt ggaaaagaaa tggaagcacg
    46741 tttaaacctg aaaaactggc aaacccaacc attggcaatg cagccatcag gctttacttc
    46801 aacaagccta ttgaaaatcc cattgttact ttcacagtaa agaatgaaaa caatgagccc
    46861 gtgcaaaacg ccagggtgta tgttcgtgag gttgattcaa cccttataac gggggtttca
    46921 ggcgaagcat caattgaact ttccaacggt acctacacct gcaggctcga ctccgctggc
    46981 ttcgcgccag ttaccaagca ggtaaaggtc ttttaccagg acctattcat tgattttaaa
    47041 ctccaaaaaa cctaccaggc taaattcaag gtgctggacg aagacctgct aactccaatt
    47101 gcaggagcag aaatcaacat actgtctgaa tcaattgata ttaccttccc tgaagagccc
    47161 tacctaaccg accaaaacgg actggatagc ctccaaattg cccctggata ctacaacttt
    47221 tccgtcaggg cttccggata taacatgagc agccccggct acttttttat cgaacccagc
    47281 gataccctga ttgaagtttt catgcaaccc gcaaccaccc acagcctaac cctgcaggtt
    47341 attgatgaaa agggaacctc tctacccggt gcccaggtta caacactgaa ccacggggct
    47401 aaactaacta acgataatgg tgaagcatta ttttcaaacg ttcctgaagg ggaaatatcc
    47461 tgcatggttt ataaggaaaa ctacatcaac aatgttgcag gcgttaacct aacctccgac
    47521 agcactgtta ccgttttact ttatgctaac cgctacaccg ctactttctt cgtaaccagc
    47581 aagaacgtgc ctgtttacaa tgcccaggtg attgttgagg ggattgatac tgcttacacc
    47641 agcgctacag ggtatgccaa tatcaacctt ataccatttg gtagcaacat tcgctactca
    47701 gttaagctca acggctacaa cacctacctt ggaacgttta acctggagga tagaaatatt
    47761 gttgtgaatg taccgctaac cctcaccacc atagaaccca ccaaaactaa ctcgtttgca
    47821 atctacccca acccttcaaa cgggaacttc agcataaacc ttgaaggtag ctttagcgtt
    47881 gccatttaca acttggcagg atacaaggtt tacctcagca accacgccga gaactcccta
    47941 caggttgaca tacacagcct gcctgcaggt atatacattg ttagggtaac cggtaaaaac
    48001 ttttcaacgg ttgcaaagat tatcaagaag taaagcaaat ggctacgaaa agctaaaagg
    48061 gcagcaatgc ccttttttta gttttaggta taaaatgttt cgaccacccc acatgggttg
    48121 tgccaggtat aagaaagaat agctgtatat ttacatttta taacctttcc tatccaaatt
    48181 tgacaaaaag agttaaaaaa catcaccaat tggcaataaa ttgttacaga aaagtgaact
    48241 ttcttttaag agttaataat tttgattaac attgtggcat aaaaaggaac cgtaaaaagc
    48301 gctcattctc caggtatatt ttggtatggc catatctttt acttacgtaa aactagtgaa
    48361 gatgcaatac cagcgcacgg agtaaacgta tgatggtttt ctgcatctaa gaaagagcca
    48421 tgttggagcc aaatgagatg taatagccag taaacatgga agctgcaatg cgatataagc
    48481 caatacgttt ttaaacaccg aaaaaacaat accatcatga aggaaacgcc aataccccgc
    48541 gaaattgtta agcagaagat tgctgaatcg aatcttcccc gggtggggag aggttccatt
    48601 cgtgaaattg tgcatctggt aaacgagatt gaaaaggcct caggacaaaa gttcatccgg
    48661 atggagattg gcgatcccgg acttcagcct gctgaaattg gaattgaagc cgagattagc
    48721 gctcttcgaa gtggggtggc ctcaaagtac cccttggttg atgggatacc acctctaaag
    48781 aaggagatat cccgctttgt gaaaaacttc atcaacattg atgttgatga aagaggttgc
    48841 attcccaccg ttggctccat gcagggagga atggcggcat ttctgatgac tacccgctgc
    48901 aatacccgaa aaaataccgt gctctttatc gacccggggt ttccagtcca gaagcagcag
    48961 cttaaggtgc ttggtattga ctatgaaacc tttgatgtgt ataacttcag gggcgatagg
    49021 ctgcacgaca agctggaatc gtacctgaaa aagggaaata tttccaccat actctactcc
    49081 aatcccaaca acccctcatg gatatgcttc acggaaaaag agctaagaat aattggagaa
    49141 ctcgctactg cctatgatgc tatagttgta gaagacctgg cctacctcgg catggacttt
    49201 agaaaagacc tgtccaaacc tggtcaacca ccctaccagc ccagtgtggc caactatacc
    49261 aacaactggc taatgctgat ctcaagctca aagatttttt cgtatgccgg acaacgaatt
    49321 ggatgcatag tggtaagcaa tgaactatac aatcgccatt tccccgacct taaacggttc
    49381 ttctcgtccg atgagtttgg ctacgccatg atatatggtg ctgtttaccc cctatcgtcg
    49441 ggtgtttgcc actcggcaca gtatggattt gcagccatgc tgaaagcagc aaatgacgga
    49501 gtttacaatt ttgtcaacga tgtaagggag tacggcgaaa aagccaaagt catgaagaag
    49561 cttttcaccg acaacgggtt taaaatagtt tacgacaggg atgaaggcga accgcttgcc
    49621 gatggcttct actttaccat atcctacccc gggctaaccg gcgaagagct aattgaggaa
    49681 atgctctact atggcattag tgccatttcg ctcctaatca caggaagcga acgttcggaa
    49741 ggcttgcgtg catgtgtttc acaagtgcag agaagccagt tttcggactt agagtaccgc
    49801 cttaaaaaat ttcacgaaga tcatccaacc aagtaatatt atagcaagct aactattata
    49861 caaacacttt taaatttttc aatgtcatgg caaaaataag aggagcaatt gttgttgatg
    49921 tggaaaggtg caagggatgc agcctttgtg ttgttgcctg cccttcgagc gtcattgcgc
    49981 tggcaaagga ggtaaacggc aagggttaca actatgccta catggtcaac cccgatgcat
    50041 gcacaggatg tgttaactgt gccattgtat gtcccgacac tgttatttca gtttacaaag
    50101 ttaaggctga tgcagtagcc tagagtagca ttacttaaag ttgaaaatac aaatttcaaa
    50161 agaaatggca gaactaaaac taatgaaggg gaacgaagct atagccgagg cagctatccg
    50221 atgtggttgc gacgggtatt ttggctaccc catcacccct caatcagaaa tcatggaaac
    50281 ccttatggcc agacagccgt gggatgaaac aggaatggtg gtgcttcagg cagagagcga
    50341 ggtggcatcc ataaacatgg tatatggagg tgccgcttgt ggaaaaagag ccatgacctc
    50401 gtcgtcaagc cccggtataa gcctaatgca ggaaggtata acctacattg ccggatctga
    50461 gctgccctgt ctaatagtta atgtagttcg tggagggcct ggccttggta ccattcaacc
    50521 tgcccaaagc gactactttc aggctgttaa gggaggcgga catggcgact acaagcttat
    50581 tgttctggct ccggcctcag tccaggaaat gtacgatttt gtagatttag catttgagct
    50641 ggcctttaaa taccgtaatc cggccatgat tctctccgat ggcgttattg gccaaatgat
    50701 ggagaaagtt caaataggcg attacaagcc gcggcttact gacgaggaag ttgtaaaacg
    50761 ttatggttcg tgggcaacca ccggtaagcc ttcaacccgt gagcgcaaca ttatcacctc
    50821 ccttgacctt gatgccaaca gccaggagct gttcaacctt aagctgctta agaaatatgc
    50881 ggaaatagaa aaaaatgaaa tccgtttcga ggaaatttcc tgcaccgacg cagaatactt
    50941 gctggttgct tacggctcat cgagcagaat atgccaaaaa accgttgatt tggcccgtga
    51001 aaagggaatc aaggtaggat tgttacgacc cattaccctt tacccttacc cctatggagc
    51061 cattcaaaag cttgtgccac aactaaaggg aattttagct gtcgaaatga gcgccggtca
    51121 gatggtggag gatgttcgac tagcggttaa cgggaaagtc cctgttctcc actacgggag
    51181 aatgggtggt gtaataccca ccccaaatga ggtgttaaag gttctggaag ataaacttat
    51241 taaaggctaa aacgatggac gttaaggaca taatcaaaga ggaaaatttg gtttacagca
    51301 aatcaaaagt tctcaccgat acaattatgc actactgccc gggctgcacc catggtgttg
    51361 tgcacaaggt aatcgccgag gtaattgaag agctgggcat tcaggataaa accatagggg
    51421 tttcaccagt gggctgtgcc gtatttgcct ataactacct cgatatcgac tggcaggaag
    51481 ctgctcatgg tagagctcct gctctggcta ccgccattaa ccgactctac cccgataagt
    51541 atgtgtttac ctaccagggt gatggcgatt tagcttccat cgggactgca gagataatcc
    51601 atgcatgcaa ccgtggtgaa aacatagtag taatattcat caacaacgga atatacggta
    51661 tgaccggtgg tcaaatggcg cctactacgc tgatagacat gccgacctcc accacgccct
    51721 acgggcgtaa agttgacctt aatggttacc cgttaaaaat taccgacctt gtggcacagc
    51781 tgcccggtac atgctttgtt accagacatt cggcccacac tccagctgcc gttagaaagc
    51841 ttaagaaagc cgtaaccaaa gcttttcaga atacccgaag gaaaaaagga gtatcgttca
    51901 tcgaagttgt ttcaacctgc aactcgggat ggaagcttag ccctgttgaa tcgaacaact
    51961 ggatggttga acacatgttc ccctactatc cgctgggcga tatgaaagat gagtaaccgg
    52021 aaacctatta aaacactacg aacatgacag aagaaatcat catagccgga tttggaggtc
    52081 agggggttct ttcaatgggc aaaattttgg cctactccgg aataatgcag gatcaggaag
    52141 tatcgtggat gccatcctac ggccccgaaa tgcgaggagg aactgctaat gtaaccgtta
    52201 tccttagcga ttcacgaatt agctcaccca ttattcagga atttgacacg gccattatcc
    52261 tcaaccaaca gtcgatggac aagtttgaac caatggttaa accaggcgga ttgctgattt
    52321 acgatcccaa cgggattacc cgccatccgg ttcgtaagga tattgccatt taccaggttg
    52381 aagccgccga ggaagccgcc aggacaaaaa atccaagaac cttcaacatg atagtgctag
    52441 gggctttcct aaaggtaaaa cctattgtta aacttgataa tgtgataaaa gggttggaaa
    52501 aatcgctacc cgaacgccat cacaacctaa ttccggtgaa taaggaggct atcctgaaag
    52561 gaatggaact agcaaaaaaa tacagctagc taaagttgaa tgttaatggt attctccact
    52621 aaaagcccca gcatttgggg cttttttcta actcaaataa cgcatatctc aatttcgaac
    52681 tagcatttac ttcacttacc gaacgcagct atcaatccta atgctcataa ggtgaattag
    52741 caaaccaaaa tttatttgtc agcattagca gtgagcgttg tgccataacc gtaaatcttg
    52801 ttttttatga tgtaatccca cacatcatcg ggaacgtatc cggaagcatc tacaccatgg
    52861 gcaatcaggt ggcgaatttg agttgacgaa atccctatta caggagcgtc aataagggta
    52921 aagcgcccgg aaggtaaatt tttaggatta aaggaacctt cacggggata gacataaaaa
    52981 tcccagccat ttatcagggc ggcggattcc ttccaatcat ggataccagc aatgttatcc
    53041 gaacctaaga gaatcacaaa agtgcacccg gggctttcct gctccagtct cctgagcgtg
    53101 tcaattgtgt aggagggtcg gggcattccc atttcaacat cgcacacctt catccgggtg
    53161 ttcttttcgg gaatagccag ccttgtcatg ttaaggcgat gctcatcggc agcaagctca
    53221 tcaatgcttt taaaaggatt ttgagggctt acaacaaacc aaatctcatc aagatcggaa
    53281 aattcaagaa taaaatcggc tataaccaga tggccgatgt ggatggggtt gtaggaacca
    53341 aaaaataagc ctattttcat gtctggtcaa tttttatgaa atccctgaca attctttcag
    53401 cctccataaa agccttctct acctgattgt taattaaaac aacatcaaac tggctggcat
    53461 acccaatttc ctcctttgct ttgtttagcc ttagtgctag cgattcatcg tcttcggagc
    53521 ctcgttttat aagcctctgc ttaagatcct ccatagaggg gggcatgaca aacacagcca
    53581 gggagcaatc gggaaatttc tttttcaggt tcaatccgcc cttaacatcc acatcaaaca
    53641 ccacatgatt tcccttgttc catattctat gaatttcaga ccataaggtt ccgtagaaca
    53701 agttcggata cacctcctcc cattcaacaa actcgttgcc agcaatacgc ttcttaaatt
    53761 cctccaccgt gaggaaatgg taatctactc cgttaatctc acctttcctg ggaggccggc
    53821 tgcatgccga aaccgaaaat tctaactcgg gaaagcaatc gagcaggtgc ttaacaatgg
    53881 ttgttttgcc cgatcccgaa ggtgctgaga atataagtag cttgccatcc atgccgttaa
    53941 gtgaggagta atagtagcaa agtttagaag aatttacaaa cggttaaaga atattgagcg
    54001 attgctcctt gattttctcc agctcatcct tcatttgcac cacaactttc tgaatatctg
    54061 aatggttggc cttagaaccc atggtgttga tttccctccc catttcctgc gcaataaagc
    54121 ccagcttcct tccaacctgc tcatccaacc ccgctgtttc caggaaatac ttgcaatggt
    54181 ttttcaacct taccttttcc tccgtaatat cgagcttttc aaggtaatag ataacctcct
    54241 gctcaaaccg atcggaatta atctttacat catcttcgag cgtttttaac gcctcccgaa
    54301 gccttttctt cacatcctcg attcgctgct tttcgtaggg ttcaaccttt tgaagcaaat
    54361 caagaatgag caaaaccctg tttttaatat cgttatacag cacgctgccc tcctgcctgc
    54421 ggaagtttac aagactctct accgcattgg agcaaagttt tttaactgaa ttgagctcat
    54481 cctcacccac aacatccttg ccgttaacac gaagcacatc gggtaacctt agaattgtct
    54541 gcactagcga ttcgtttgta atgtcaattt cgaactgacg ggctatttct ttaatctgca
    54601 gcaggtagcc ttcaaaaata tccctgttaa ttggggtagt tgccgattcg gctgtttcct
    54661 caaaactcag ctgaaagtca acctttcccc tgaccagctg ctgcgcaata atggaacgga
    54721 tatccatctc aatgcccctg tagatggatg gaatcctgat gcttaaatcg agctgcttgc
    54781 tgttgagcga gcgaatttcc gctataagtt ttttactgcc cagctgcatt tccgcccttc
    54841 caaaacccgt catcgaaact agcataattc ctgttttaat ttgtacaaag gtaatttaga
    54901 attattcatt atagccaacc agcgcacgaa atcatcattc aagctgaaat tcatgttttt
    54961 aacggccggt gctcctctcc aaacactttt acctggtttc gatgtaaact acagtaaggc
    55021 ataattaccg gtggaatgct atctttgcac ccgcaaacaa tgtaaaagta aggtatatgg
    55081 caaacagagg gttgatggca attttactgc ttgttatctc gaacaccttt atgaccatgg
    55141 catggtatgg gcatttgaaa ttcaaggagc tcaaatggtt tcaaaatttt ggtatagttg
    55201 gcatagttct gatcagctgg ggtatagccc tcttcgagta tttttttcag gttcctgcca
    55261 accgtttagg ttacaaggaa aatggcggcc cattttcact cctggagcta aaggttattc
    55321 aggaagtgat taccctgctg gtatttataa ttttcaccgt gctattcttc aaaaccgaaa
    55381 gcataagaat aaaccaccta atcggctttt gctttatggt gctagcagtt tacttcgtat
    55441 tcaaaaagtg atgggttgtg ctggtactaa aacgaagaag gcctttcggc cttcttctcc
    55501 ccaaacacac gatttactga aaaaaccaac tggtttccat caaactacct agaaaaaata
    55561 tacaaactaa cctcccagta aactatggaa accaaactga ataggacaaa tataatacca
    55621 aatttttatt gaatgaaatt ataacgaaaa ttattactac gaaaaaattg aaaaattttt
    55681 aacgactgtt gtttattatg aaccacaccc tgtataatta aaattttcaa ctttcccttt
    55741 gccatttatc tacttatacc ggtacttcgt tttacgtttt aggacattaa tcaaagaaaa
    55801 ggccttacag tttgcccaag ctgtaattat gtaatatctt tacgctgata agcaaaaata
    55861 aatatggatt ccctatatat tgaagcagaa atagactccc ctgaagttta ctttgacccc
    55921 gaaaccaaga ttttttccat ttcgggcatc tcacaccccg agaatgccaa agaattttac
    55981 caggttatca tggagtgggt tgacgagttt tacgagtaca tgaaaaacaa gaagccttgc
    56041 aagattatag tggatatgaa ctttaaatat atcaactctt catcgtacaa gtacctaaga
    56101 gagctgctca tcaaaatgtc taactttaaa aacagccact ttgatattga ggttgtatgg
    56161 aactaccagg aagaggatga agacctgcta accgagggca ttgttctttt cgaattaccc
    56221 gaaataaacc tgccctaccg atgtgtatca taccgttagc ataatcggcc aactcacaac
    56281 ccggctacgc tgcccagtac tgcattacat ttgaccaagc caacatttta atgaatgctg
    56341 tcaatcatcc atttattaag gatgaaatat aaatggtcaa aaatccaatt tttatacctt
    56401 tgtgctgttg cagccaacca ttttcatggg attttcagcc caatatttga tttaccccgg
    56461 caaaatcgaa gttgaaagaa ggctgtcagc caaataacag ggctatggga tagcatgggc
    56521 tctaccactt gaaaaattaa agaatacaaa gcttatggca tttatagagg aacttttagc
    56581 aaaagaatca tacacaaaaa gtgagctggt agaactcttg caggttaccg gtgatggaag
    56641 agctaagctc tttagcaaag cggccggaat aaaagaaaag tatctgggta ataaggttta
    56701 cctccgtgga ctggttgaaa tgtcgaatgt atgtcgtaag aattgcttat actgtggtat
    56761 cagatcgggt aaccaaaagg ttaatcgcta caaccttacg gacgatgaaa tagtggatgc
    56821 ggcaattttt gcctggaaga ataaatacgg aagcattgcc ctgcaggcag gtgaaattag
    56881 cacccgcgag tttgcaacgc gggttgaagg cttgctcggt aaaattaatg ctgcaacata
    56941 cggcgagctt ggcatcaccc tttccttagg tgaacagaat gaggacacat accgacggtg
    57001 gtttcaaaag ggaggaatac gctacctgct gcgaattgag agttcaaatc aggagctcta
    57061 caaaaagctg caccctaacg atagcctgca cgattacaaa acaagaatag attgccttaa
    57121 aagcctccgt cgggttggct accaggtagg ctcgggcgta atgatcgggt tgccattcca
    57181 aacccttgaa catctggccg acgaccttct ctggatgcag gagatggacc tcgacatggt
    57241 gggcatgggc ccctacgtgg agcatcctga tactccgctc ttcgagtacc gggaccagct
    57301 gctaccagtt gccgagcgtt tcgacctctc gctcaaaatg acggcgctaa tcaggataat
    57361 gatgaaaaat attaacattg ttgcgccaac cgccctacaa gccatagatc ctcttggaag
    57421 ggaaaaagcc attaaggtgg gggccaacgt ggtgatgccc aacattactc caggagcatt
    57481 tcgtaacgac tacaagctat acgagaataa accctgcgtt gacgatgctg ctgatgattg
    57541 caaaaattgt ctggatgccc gcatacagct tgccgggcat gaagttggat atggcgaaca
    57601 tggcgattca ccgcacttct accagagaaa aaatgcccaa taaat
//
LOCUS       DAOSHM010000011        54535 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_449_length_54535_cov_20.660004, whole genome shotgun sequence.
ACCESSION   DAOSHM010000011 DAOSHM010000000
VERSION     DAOSHM010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 54535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 54535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54535
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_449_length_54535_cov_20.660004"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(31..981)
                     /locus_tag="PK839_03105"
     CDS             complement(31..981)
                     /locus_tag="PK839_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009275525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOG71757.1"
                     /translation="MQKQMDDVLWFNRVGDVAFVDKVYIYGPPPANVKNPTAMGATNP
                     VKFWAYVFIPKYIDPSKKYPLLVFPHGGVHADFTTYHTHIIREMMAQGYIVVAAEYRG
                     STGYGRSFWENIDYGGLENEDVYASRNYMVENYDFVDEKRVGIIGWSHGGMITLHNIF
                     DHPNDYACAFAGVPVSDLIMRFGYSTDDYRELYSAPYHIDKSPREDIAEYKRRSPVWQ
                     VDRFKNTPLLIHTNTNDDDVYMIEVEHLIQALKAEGKKFEYEIFKDMPGGHSFDRIDT
                     KAAREIRVKIYKFLARYLTPPNPIRSVDDINRAAYLLHNP"
     gene            complement(1220..2476)
                     /locus_tag="PK839_03110"
     CDS             complement(1220..2476)
                     /locus_tag="PK839_03110"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOG71758.1"
                     /translation="MTFHLNPASYLSKIKFAFRALKSPNFRIFFYGQAVSLMGTFIQG
                     LALGWLVYRLTNSPFLLGAVSFAGQIPSLVLMPFAGVYADRLNRRKVLVITQGISMIV
                     ALTLATLYFTHTIQVWQIFVTSILNGIAIAFDAPFRHSFMLEMVGDKDLLQNAIALNS
                     TLVNSARFIGPAIGGVLVAWIGEGFCFLVNGISFMAVIASLLHIRVAQVERKPHNSSI
                     FKELKEGLSYSYNFLPIRYVLSLLVVVGFLGFPFQTFMPVFARDILHGNSQMLGLLTG
                     AVGAGALTGAILLASLKSIKVIPNIILYSSLTFGTSLAAFSLSSYATLSVGLLFFTGF
                     GMIVQLAATNTLLQYIVDDDKRGRILSLYSLSFNGFPPLGSLLLGAISPSVGIQPTMA
                     VAGGLCAISALLFSRRVPVVKSVVPD"
     gene            2721..3185
                     /locus_tag="PK839_03115"
     CDS             2721..3185
                     /locus_tag="PK839_03115"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HOG71759.1"
                     /translation="MKKVKTIALVAHDHRKRDLVEWVEWNWQELLKHKLICTGTTGRL
                     IEESLQKKKSDVADHIQLTRLKSGPLGGDQQLGALIAEGKIDILIFFWDPMEAQPHDV
                     DVKALLRIATVYNIPTATNRSTADFLISSPLIDEDYNPILFDYSHYMNRKVF"
     gene            3583..5259
                     /locus_tag="PK839_03120"
     CDS             3583..5259
                     /locus_tag="PK839_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG71760.1"
                     /translation="MNHNKGKWCRTGSMVLLTALLTGCYDFSNFENFKVEPVNPSIVA
                     PVVNSTITFKELAETEDANTIVIVKPGDTRFYLSFRDTMEIANASGEFSIPPFAYSKT
                     YQPDAGEIPPVAPPAGESITFSPKTFTQTWQPDADSELKLIKLSQGSMNITVTNNFGN
                     IISGSITLNFLLTPQNQPYTINIPNINPASSQAISLTNLAGYSFNLFDGTSYNTLTFT
                     ATITLTSMGNSINPGDNINIDISANNLDFTEIYGKVNKTINLSDQSFPLDIFRSTYIA
                     DQHLAEPSLMFKFANSYGIPLSLNVNQLVASNDQTGSMNVVNETVPPDGSLLIGAPNS
                     LNYVGSPSQEFAMDSLKLDYQNSNIEDVFDFAPNNFTLKSGITLGDASTSHDYFVRKN
                     SNLTLFSNVEVPLVGWVETNGIRDTVEIDLPDIENDLNMEDDDELSLTLKFRFNNGIP
                     LNVYFQAYFIDDYDHELTRLYTNVAEEQLIESAAVNPSTGKVTTPNVKISEISIDRNR
                     YKQMQGATKLVLQVRFKTGGSTHQNIVVESTNSIDIQMSIKAKGTVRFDS"
     gene            5450..6802
                     /locus_tag="PK839_03125"
     CDS             5450..6802
                     /locus_tag="PK839_03125"
                     /inference="COORDINATES: protein motif:HMM:NF040164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5723 family protein"
                     /protein_id="HOG71761.1"
                     /translation="MKRLSLLALILTVGIYAKAQSELTLPFMPGVFQSSFVNPTVIPE
                     HTFSMGFSIYTQVVTNGFVPNNVLIFRDDTMHFDPNLLLDDLRDKNLFYMGENTDIFH
                     IRTKILNGYYWFAIRQRMESSFFYPYDLFRMVIEGNEPYIGQTLDFSNLKIFTSLFNE
                     YSFGMTKEYPHWIFGGRLSLLQGLSNINFNPTRLSIKVEDEMYGQLANADAQLRTSGI
                     PKDGNGDPSFDHVEEPEWITDYFTNFKNKGFALSGGATYRYDDRTRISLSFRDVGFIT
                     WKDSIETYTLNGNTNFTGLDIMSDLLYGNDVEVDSIFENILDEDFHRDTIYNKYTTWL
                     NPKFNLTASYDLARRTTVGFSFSGVVNQKLYTALTLSLSQGIGRFFSIVGTASINQRS
                     FTNLGLGLMLKPGPLQIFVVADNLYAAHNPLTTTNFNIRVGVNLVFGRINQPEGLPFR
                     "
     gene            6846..9212
                     /locus_tag="PK839_03130"
     CDS             6846..9212
                     /locus_tag="PK839_03130"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOG71762.1"
                     /translation="MRVSGIKNKIVALLLGSMVALSLQAQTSFTPINLGDRVNSEYPE
                     INPILHPDGKTLYFSRANNPENTWGGVNSQDIWYSVLGDDGTWSQAQRLPRAVNIGRY
                     NALFAILNNGKTFLINGHYSPNGKYWIERGLSTVEQVENENWGKPQSIEVKGYSNKNR
                     GKTTTIFVSPDEQKLFMAFSKKVNGKKASIYLSERKGDRRYSKPKKINFLGNNKCESM
                     EAPFLTSDGNALYYSCKINGDNNLYMSHRTDDTYRSWSEPIPLNDTINTPGWECYYHL
                     NSKGSWAYFCSNTNSLGKSDIFRVKIFEENPFVRLHGLVLNKADQKLMLADTSYILKV
                     NGSEPEKLKVDRAAASYELLLPFGQSYQLQPEMKNWIGIPELYDATGIKEFTDVELNL
                     YFEPIPYVKIFGKVINTRTNLPISPEIGFKVLINGLENDSVKYIRDISGYQALLPLGQ
                     KYVVSLNLPNYRSEADTVDVSNISFYTEKQVDFHVTSAPWVEVYGIAMDNVSFTPIPG
                     SSSPKLLVNGYPADSIAIDPFTGEFTIKLPYGIKHNLAISSKDYNPLDNVVDLTGYVE
                     FARINHDVYAERKDANMATLYGKVINLKTGQPVESSIPVKLKVNGAETRAFIYDSTTA
                     AYTLKLPVGVSYDILPSVKNFYNKYEQVDLTKTKKGTKVPKNFYITPIEVGQAVNIDF
                     IYFETGKSTLKPQSFRSLNALVDFLNEYPNVIVEIGGHTDNTGSAAINEKISNLRAKA
                     VADYVVSMGIPASRITSKGYSFNKPKASNATATGRAQNRRVEFTIVGI"
     gene            9417..10040
                     /locus_tag="PK839_03135"
     CDS             9417..10040
                     /locus_tag="PK839_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018335997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HOG71763.1"
                     /translation="MFALKLKLLLSCFVAVLALVNPIQKVFVMTSLQDQFSEADTKMV
                     ALKSSLTALLILLFFLLLGEVVFNYVFHVELYAFQITCGVVLFYNGITGLQKGIFIKV
                     DQNISIHDITAVPIAIPMIAGPATITAAVTFPAHYGHFNTIVSIVAALAINLLFMLYA
                     KHIARFLNRFNLMNPLVRIFGLIVATIGVQMTLNGIWGFVVSVAGHV"
     gene            10112..10933
                     /locus_tag="PK839_03140"
     CDS             10112..10933
                     /locus_tag="PK839_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011966872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOG71764.1"
                     /translation="MEQKNFIDIDQVIASKSPTLQKSLPRFIVNYLKRIVHQDEINEI
                     LAKHGHKQGAEFVEAVLSTMGVNYSVEGLENLQDNQRYLFASNHPLGGLDGMVLIHLL
                     AKRFDEVKFPVNDLLMHIKPLSSVFIPVNKLGTQSASNAIQLEEAYSSSAQILYFPAG
                     LCSRKQKGRIEDLEWKKNFIVKAIKHERDVVPVFFDGRNSTFFYNLARLRAKLRIKTN
                     IEMLYLSDEMFRQKGKSITVKIGKPIPFSTFDNTRNAHEWAQWVKHVVYQMKPAS"
     gene            10995..11954
                     /locus_tag="PK839_03145"
     CDS             10995..11954
                     /locus_tag="PK839_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOG71765.1"
                     /translation="MQEIIEPVDRNLLMAELTPEKFVRNTNNAENELYLFTAHDSPNL
                     MREVGRLREFTFRSAGGGTGEEVDIDEYDICDNCYKQLIVWDPKEREILGGYRFHSVD
                     PSKGDEINLSTKHLFNFSEKFVREYIPKMIELGRSFVQPKYQSLSRGGKGLYALDNLW
                     DGLGALIVLNPHIRYFFGKVTMYRSYNVEARDTLLYFFEKYFKDNEQLVTTIHPIEVN
                     MDRDKMERLFTGKNYTEDYKILSHKIRELGEKIPPLINAYMNLSPSMKVFGTVINPYF
                     GNVEETAILITIPDIYQHKMERHTGTFVRKLAGKYLRPYRKES"
     gene            complement(12039..13940)
                     /locus_tag="PK839_03150"
     CDS             complement(12039..13940)
                     /locus_tag="PK839_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HOG71766.1"
                     /translation="MSEQIKHECGIALIRLLKPMEYYHLKYGTWQYGLQKLYLLMEKQ
                     HNRGQDGAGLTSLKFDLEPGKRYIDRERSNSDSSIKDIFDAVNRSINQYGHRPELAGD
                     SAWAKENIPFAGEIYLGHLRYGTFGNNSIDYVHPVMRDNNWKSRTLVLAGNFNMTNVD
                     ELFNKLVSLGQHPRDYSDTVTVLEKIGHFLDEENQQVFRQYKNEGYSNMEISKLIAEN
                     INIGKILQAATKDFDGGYAIAGLIGHGDAFVIRDPWGIRPAFYYLNDEFAVVASERSA
                     IQTVMNVKTDDVKEVNPGYGLIIKRNGTITHEEIRVPQQRRSCSFERIYFSRGSDRDI
                     YQERKKLGELLTPAILKAINYDHSNTVFSYIPNTAETAFYGMVKGVEDYLKVEKKRMI
                     LEKSSSLTESDLDEVIAIRLRVEKIAVKDVKLRTFISQDKGREDLVGHVYDITYGAIR
                     PGIDSLVVIDDSIVRGTTLKESILRILDRLNPVSVVVVSSSPQIRYPDCYGIDMAKLS
                     DFVAFKAAVGLLHDTGQEAIINKVYRKCKEQQYLPKEEIVNYVKEIYSPFTDDQLAEK
                     IAQLLTPKDMRAKVKIVYQSIENLHLACPNDLGDWYFTGNYPTPGGNRVVNNSFINFI
                     EGRNNRAYQ"
     gene            complement(13986..14957)
                     /locus_tag="PK839_03155"
     CDS             complement(13986..14957)
                     /locus_tag="PK839_03155"
                     /inference="COORDINATES: protein motif:HMM:NF014798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HOG71767.1"
                     /translation="MEQFRNKGDKATRTGFGEGVVDIGELNPNVVVLGADITASVGLD
                     GFKRRFPERFFSFGIAEQNITAVAAGLALAGKLPVFSSYGVFAALRAADQLRISICYN
                     NVKVIIGGAHAGISVGPDGATHQALEDIAYLRALPNMTLISPCDANQARAATIAAMQQ
                     VNGPVYIRFGREPVPNFTDEDQKLVVGKGQVLMNGDDVAIVATGHEVWESIEAAHILA
                     GQGVNAMVVNIHTIKPLDIPLLVDMAHHTGFVVTVEEHQVNGGLGGAVAEVLGKYCPT
                     PMEIVGMPDCFGESGTPAELLQKYGLNSQSIARKVLETLSRNKKGIL"
     gene            complement(14938..15783)
                     /locus_tag="PK839_03160"
     CDS             complement(14938..15783)
                     /locus_tag="PK839_03160"
                     /inference="COORDINATES: protein motif:HMM:NF012670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOG71768.1"
                     /translation="MHQLLKVSKQLREDVVRSLASAGSGHLGASLGLADIFTVLYWQV
                     LHHRPLQPGWSERDRLILSIGHAAPVLYAALANSGYFPREELLTLRKLGSRLQGHPGR
                     DHGLPGLELSAGSLGQGLSVAVGLALAAKIDRAPWRVFSIHGDGELQEGSIWEAAMSA
                     AHHKLNNLIALVDRNDCQIDGRTSEVMGLEPLRAKWESFGWRVLECDGNDIAQLVEIF
                     EVAEHGDVKPTVIIARTLMGYGVKSIQDDYRWHGKAPSPTEAEKFLDELHAHYLSLQN
                     GTVQE"
     gene            complement(15803..16264)
                     /locus_tag="PK839_03165"
     CDS             complement(15803..16264)
                     /locus_tag="PK839_03165"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOG71769.1"
                     /translation="MRSLPALLFNSLFLICVFAAEHVHGQGVSGIVQNQHEGNEQGKV
                     KITQDPVVSNLLELHILQNAKNPGMNGFRIRIFLELGQNARRNSEAVMNAFMEKYPDI
                     RVYQSYDNPYWKISVGDFRTRESAQKLYHQLLVDYPKAFIIPQWINFPPLE"
     gene            complement(16303..16830)
                     /locus_tag="PK839_03170"
     CDS             complement(16303..16830)
                     /locus_tag="PK839_03170"
                     /inference="COORDINATES: protein motif:HMM:NF015147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HOG71770.1"
                     /translation="MKVKRFLAAFVPIFMVLMSGCAPKFQDIKINGINKVEYNGLKND
                     VVKLNLAVGIENPNSKRITVKEVEFKSWIKQRELGTVRVKNRIEIPPNSSQIYSIPVE
                     VKLNSSADALKLMISGEKILTMITVEGFVKGSSFPIVKKIKIPRQPLNNLINKYQNGF
                     IITDTISVRNSTVGN"
     gene            complement(16950..17696)
                     /locus_tag="PK839_03175"
     CDS             complement(16950..17696)
                     /locus_tag="PK839_03175"
                     /inference="COORDINATES: protein motif:HMM:NF001030.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOG71771.1"
                     /translation="MSGHNKWSTIKRKKGALDAKRSKEFSRIIKEITVAVKESGSDPD
                     GNPRLRLAINNAKGVNMPKDNIMRAISKAEKDPENFHELTFEGYGPGGIALFIECLTD
                     NNNRTVSAIRSILTKRGGSLGTNGSLSFLFDRKGIFTIAKDKLKLDEVELDLIDAGAQ
                     SIDDNGDFYVVTTEMEDFGRMNKKLEELNVEVENASLQRIPNDYKTLDKDSSLRFLKL
                     IDELEDNDDVQNVYHNLEMTDEVVAAMENE"
     gene            17875..19275
                     /gene="miaB"
                     /locus_tag="PK839_03180"
     CDS             17875..19275
                     /gene="miaB"
                     /locus_tag="PK839_03180"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014776123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HOG71772.1"
                     /translation="MDFNGEEPNIFEGISTAGTGNGAKVYLETYGCQMNVNDSEVVAS
                     ILLANGYSITQEINEADIILINTCSIRENAETRVFGRIDLFGQVKKNKPSVIIGVLGC
                     MAERLKDELLEKKKIVDLVVGPDAYRELPTLLRAAESGQKGVNIMLSADETYADISPV
                     RLDKYGVSAFVSIMRGCNNMCSYCVVPYTRGAERSRDPQTIIHEVEELVRNGYREVTL
                     LGQNVNSYSWSESKGMVKHTVNFADLLEMVALVSPFLRVRFSTSHPKDLTNDVLYTMA
                     MYENICNHIHLPVQSGSDRILQLMNRKYSRNSYLNRIQAVREIIPDCAISTDIIAGFC
                     TETELDHDLTLSLMREVGFDFAYMFKYSQRPNTKAARHLVDDVPDEIKSRRLTEIIEL
                     QGKLSEESKKKDVGKRFEVLVEGTSKKSDDFYVGRTTHNKVVVFPKEASSIGDFVLVE
                     VTSCTPATLIGKIVDE"
     gene            complement(19330..20100)
                     /locus_tag="PK839_03185"
     CDS             complement(19330..20100)
                     /locus_tag="PK839_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG71773.1"
                     /translation="MSKINLTPGEINDLIDLYQTEIDRTQRRIENLKSILKKIDRAQE
                     EVSEPASRVAAPARRGRKKLVESVPAAGEAVTPEAKVDGRKVRKGRNAKAVAKSAAPK
                     VKAIRKTRKSKANAATSEETKLAATKRGRKANLKSKVAAKKSPRKESVVRKKIKSGAG
                     PDKVKWIDLIQDILRSKQNLMLANSLTVEAMERLGIDESDRDRVRMAVATNLTRLTKN
                     LKLVVKFKPEGSTNYFYGLTEWFDEGGNLKSEFQNKLM"
     gene            21522..24803
                     /locus_tag="PK839_03190"
     CDS             21522..24803
                     /locus_tag="PK839_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008671998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOG71774.1"
                     /translation="MKRKSFKTAMLLVALGSSLWAQNSSNPLWMRYPAISPDGKHIAF
                     SYMGNLFRVSSDGGMAELLTSNAAYDFSPVWSPDGQTIAFASNRYGNFDVFTIPAMGG
                     EAMRLTFHSADEIPSAFSPDGKSILFSACIQDNANNIQFPSGALSELYSVPTQGGRIA
                     QILTTPAEQAVYNPEGTEIAYTDIKGYEDKWRKHHKSSVTRDIWIYDISTGKHRKVTT
                     FEGEDRNPAFAPDGKSLFYLSEMQGNSNVFSVSLSSIGQPESVSSFTNHPVRFLSVAR
                     NGMLCYQYNGELYTQKPGSSPQKVNIQIATDQTETRVKYENLSTGITEMNVSPDGKEV
                     AFIIRGDIFVTATDYPTTKRITNTPDQERSVSFSADGRKLLYSSERDGSWKIYESSIS
                     SAEEPNFSLSTIIKEQVIVDIPEEAFQPLYSPDGKEVAFLKERTTLCVVNLETKKIRT
                     VLDGKYNYSYSDGDQWFQWAPDGNWLAVNFMETPSWPDSDIALVKADGSGEFHNITQS
                     GYDDRNPKWTMDGKAIIWLNDKMGMRSHGSWGSQFDVYSVFLTREAYDEFKLSKQEYE
                     LLKEKRSKDKKDNGAGEDKESSSDKKKEVKAKKDKEAQRKPLIIDYENLDDRKVRLTI
                     NSSDLSDAILTDDGEKLYYLSKFEKGYDLWVNNLKDNETKLLVKIDGFAGDLQIDKDK
                     KFLFVNSNGTPMKIEIATNKLSPIKFNAELETNYPRERDYMFEHMWRQVEKKFYDPNL
                     HGVDWKFYKSEYARFLPHINNNFDFAEMMSELLGELNASHTGCRYRPKPENADQTAAL
                     GVFLDYNFDGKGVKIIEITDNSPLVKANSKVKPGCIVEKIDGIAIDCNSDYYKMLNHK
                     ADKLTLLSFYNPQNGERWEESVKPISRSIESELIYKRWVRKNNATVEKISNGRIGYVH
                     VRGMDSQSFREVYSDILGKHRNKEAIIVDTRFNGGGWLHDDLATLLSGKRYVDFVPRG
                     QLIGSEPISKWYKPSVVIISEGNYSDAHGFPYVYKTLGVGKLVGMPIAGTMTAVWWES
                     QVDPTLVFGIPQMGVKDLNGKYLENQQLNPDIKVAISPEEAQSGVDSQLIKAVEELLL
                     ELNQK"
     gene            24890..25075
                     /locus_tag="PK839_03195"
     CDS             24890..25075
                     /locus_tag="PK839_03195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG71775.1"
                     /translation="MKKIALFVIAAGMISMVACKSAPKQEEATEQPATEQTQQETAQP
                     DSAAQAAETPAQPAPAQ"
     gene            complement(25161..26909)
                     /gene="aspS"
                     /locus_tag="PK839_03200"
     CDS             complement(25161..26909)
                     /gene="aspS"
                     /locus_tag="PK839_03200"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HOG71776.1"
                     /translation="MLRTHTCGELRISDVQKVVTICGWVQRIRNLGGMTFIDIRDRYG
                     VTQVVIDEKSSNELHSIVEQLGREFVVQVRGEVVERTSKNPKNPTGEVELRTSNITIL
                     NQSELPPFTIEDDTDGGDELRMRYRYLDLRRNPVKNALMLRHTMAQEVRRYLCNKGFL
                     EVETPFLIKSTPEGARDFVVPSRLHHGQFYALPQSPQTFKQLLMVAGFDRYFQLVRCF
                     RDEDLRADRQPEFTQIDCEMSFVERDDILDVFEGMARHLFHTVKGVEFDAPFRRMTYH
                     EAMSLYGTDKPDLRFGMSFKDIGAQLKGKGFSVVDNAEYVGGICARGCASYTRKQLDE
                     LTDFVKRPQVGASGLVYIRYNDDGTLKSSVDKFYSADDLMLVAKVFEAKPGDLMLILA
                     GKRNKTLPALSELRLEMGNRLGLRPKNQFAPLWVVDFPLLEWDEELQRFFAMHHPFTS
                     PHPDDIPLFQSNPAAIRANAYDMVINGVEVGGGSIRISNRELQQTMFNTLGFSDDEAE
                     KQFGFLLNAFRYGAPPHGGIAFGFDRLCSLFGGSDSIRDYIAFPKNNAGRDVMIDSPS
                     SISQQQLDELNIIIKE"
     gene            27010..27450
                     /locus_tag="PK839_03205"
     CDS             27010..27450
                     /locus_tag="PK839_03205"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HOG71777.1"
                     /translation="MNLYSHEYFMNEALKEAKVALELKEVPIGAVVVCNNRIISRAHN
                     LTETLNDPTAHAEMQAITAATTYLNGKYLDQCSLYVTLEPCPMCAGAMFWAQLGKLVY
                     GASDPKRGYSLHGNSILHPKTLVVGSILTNECSGILVDFFRGRR"
     gene            27716..28561
                     /locus_tag="PK839_03210"
     CDS             27716..28561
                     /locus_tag="PK839_03210"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HOG71778.1"
                     /translation="MYQPAQPKIVFSDIDGTLLNGSRQLSATTIQTIRKITAAYDVYF
                     ILVSARMPMAMQAIYDELCLKTPIICYNGALIMESLAGGFDNQREICSHSIDPRIPLS
                     IFNHTLEHNLHFGLYSNNSWFVSKNDVWTEREENNTCVKAVIANNMPGIISQLASAGE
                     PIHKLMVMGSPVEIDLLINSISLESRNKITIYRSKETFLEISPAQSSKAKACSLLLDY
                     LNLKPEQAVAFGDNFNDMEMLRIVGTGVAMGNASEEVKRHADKIAPPNTEDGVAFVLA
                     EFFGI"
     gene            28558..30381
                     /locus_tag="PK839_03215"
     CDS             28558..30381
                     /locus_tag="PK839_03215"
                     /inference="COORDINATES: protein motif:HMM:NF013316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HOG71779.1"
                     /translation="MTSLELLSPAKDLKTGMAAITHGADAVYIAAYRFGAREKAGNSI
                     EDIGKLTEFAHQYFARVYVTVNTIIFDNELDEVQNLINELYRVGVDAIIVQDLAILSM
                     NIPPIALHASTQMHTNTLAKLKFLEQCGFQRVVLPRELTLSEIGSYCGSTSMEIEAFI
                     HGALCVSYSGQCYMSQRITGRSANRGECAQPCRSSYDLVDEHGKILVRNKHLLSLKDL
                     NLTSQIEQLVDAGVTSFKIEGRLKDITYVKNITAHYNKILNSIISRRPEFRRASSGVC
                     ECSFDADPEKTFNRGYTQHFINGRTVNQTSFHTQKSVGKPIGIVARVNADYFEINSYE
                     AVHPGDGLCYFTTIGQLSGMLVNRVMGNRIYPRNNPTGLSEGDTIYRNSDIEFEKQLN
                     GKSARRWVGVRIAVSDNNGNLKFSATDSDGIAVEYTLNNNFDEAKDKARSVEMLIRQL
                     SKSGDSIFRVDGVAVQDYTTPKFIPISTVNEARRMLLNNLILERLKQHTRPFRDKYFP
                     AVLYPEQAITYKGNISNTASQDFLLKHGVTSIVEAVEISAPKGVFELMVTRYCIKFEL
                     GLCPSKQKASPTGQLYLQNSSTLYPLVFDCNECQMKVMSPK"
     gene            30681..31583
                     /locus_tag="PK839_03220"
     CDS             30681..31583
                     /locus_tag="PK839_03220"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOG71780.1"
                     /translation="MIQLLTPESWPEYELIDSGDGEKLERIGSQMVARPEPQALWRKQ
                     LSNEEWVKLADAYFVKSKKTKPTENEWGEWRLKSGAKEQWVVSYNQSGLAFRLRLGLT
                     SFKHVGIFPEQAVSWNYIYNTIKQLDLVETKVLNLFAYTGASSLAARAAEAKVTHVDS
                     VKQVVSWANENMLLSGMDNIRWIVDDALKFVSREVRRGNLYNGIILDPPAYGRGPDGE
                     KWTVEENLYQLLELCKQVMEPKNNFLVLNLYSMGFSPLIAENLIRDIFGVPSYEVGEL
                     FVSDRAGRKLPLGIFVRFRSGSNF"
     gene            31739..32119
                     /locus_tag="PK839_03225"
     CDS             31739..32119
                     /locus_tag="PK839_03225"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HOG71781.1"
                     /translation="MEAIKIQGTSQYPTIVLDSEAGRFEFSGNSLPEDPKAFYEPILS
                     WLDAYAQNPNPETVVRFKMVYYNTPSSKQIFQIFKKFEKMAASGHKVSVVWAYNEDDI
                     DIKDAGKDLSDHIKLPFRLESYKE"
     gene            complement(32163..33455)
                     /locus_tag="PK839_03230"
     CDS             complement(32163..33455)
                     /locus_tag="PK839_03230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG71782.1"
                     /translation="MRKILLLTAAMVCLAGYTFSQDKVLDKSGKKPKWVNGIEKDYII
                     VAGSGPDLQVAQQNALTSIKESIVTSVAQNVKSTSTIQKEEANYNNNVNIFLEKFAST
                     TTAQSGKVPFLQGISLSKVEDFYWEKLQRPDKSIIYTYHIKYPFPEFELMKLVSDFKM
                     RDNELTRQLEDLLAQVDQVESVEQIEKNMAELASLKEYFMDARVDQCNLGITRYRSLL
                     NSIELVELESSLGELKYYLRLGGRSISTTKKPVVKSECARVTSIVNNVDNVTIKYDYD
                     NCYEDPENNILVKYRFGVADVQKPFYFDVAANKASIFVSDPFNFTAISKDSTNVVSAL
                     LDMTVRSKYDSPFTIDKVVIEWKGIPPVVIDNIGQSFQGKGNHNLKLNVEQPIKIAGT
                     SSAGKTLSLISGYIYYRSNNTGESKTYRIYNHSYTTDW"
     gene            complement(33566..34144)
                     /locus_tag="PK839_03235"
     CDS             complement(33566..34144)
                     /locus_tag="PK839_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG71783.1"
                     /translation="MKMKSMIMVAAAFTFAAVLSSCGGSKKAADSSFGKEVSVPCNDD
                     EFHSDAKFFRGTGNGISQDLSTAKSKARLDANTNLARSINTTIKSVSDRYVNERQIGE
                     NSEFEQKFEQMSREVVNQQLNNVEVACNKTMQTKDGKFQVFTALQVSKDEVLNNISNR
                     ISKDQKLQLDYDKMKFEQIFNEEMNKLESERP"
     gene            complement(34217..35191)
                     /locus_tag="PK839_03240"
     CDS             complement(34217..35191)
                     /locus_tag="PK839_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG71784.1"
                     /translation="MTRRILILVATILGFTVGLGAQNSQGKTDDLGRIALAAIVPEQA
                     EGIPQSARQQLVNKMEQIAVQNGLGAAAANPRFCIVPMVNVLSKEITPTAPPMQAVNL
                     EVTFYIVDAASQNIFSQTSIQVKGVGQTEDKAYIQALKGVNVKAGQFKGFVEKGKEKI
                     IEYYNSQCDVVLKGAQALAGQKKYEEALYILLSVPDVCMECFDKSMDLSVEIYKQYAN
                     YKCTEYMAGAKAAWANMNVDKAAEYLGKITPDMECYSEAVKLADEITQKQLADGANVW
                     DFKMKQYDDSIEKDKMMIQAGKEVAQSWAYWGAASYFNWDWSWLYVKK"
     gene            complement(35220..36179)
                     /locus_tag="PK839_03245"
     CDS             complement(35220..36179)
                     /locus_tag="PK839_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016270588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6175 family protein"
                     /protein_id="HOG71785.1"
                     /translation="MKKLLFSSILIFSTLAVFGQAKKPVIMVVPSDQYCISRGYKMVF
                     DNQGMKQTLPDYKTALQTDPDLRLVITKMGQIMADRGFPLKDLEQEMKNLEQERAESS
                     MLTSSSSGSEMAESPIDALKRVAKADIIMDLDFEVKRQGPQKYIVFNLKGLDAYTAKQ
                     VSGVAGAGNPSSAASPELLLEEAVLSHMDNFNAGLMRHFEDMFANGREVKVQIKVWSN
                     WGENLESEFGDNSEELSTIIENWFADNCVQGRFSLSDATENFMKFEQVRIPMMRTDDK
                     GRERAVDTRSFVSDLRKYLNGMGIPSKLYLRGLGEAWLILGEK"
     gene            complement(36200..36721)
                     /locus_tag="PK839_03250"
     CDS             complement(36200..36721)
                     /locus_tag="PK839_03250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG71786.1"
                     /translation="MKGKLTTMLAVVLAWVIVFPANAQTRKERKILYNSQYNYEIEPV
                     GVGQDGTKVFKVWGYGKDVGAAVMNAKEAAVAACIFKGLPGGAGSAPTPPICSQPNAE
                     VTHADYFEKFFEVGGKYLQYIAVTNDGDPSGEDRIKIKKGYKVAIYVQVMYDALRKQL
                     EADGIARRLDSGF"
     gene            complement(36741..38393)
                     /locus_tag="PK839_03255"
     CDS             complement(36741..38393)
                     /locus_tag="PK839_03255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG71787.1"
                     /translation="MKIKLLFLNAMLIMAVLGAKAQNAEQPKKIPSTYDRSSITFLYM
                     KVPGENHVQEVESKIGSIVFSDKFYNNNMETLSFDAPFSRTSTEVVPQEAVKKYLTDQ
                     KVAKSIISLWYNRQEDGSMSMDKIFDRGMFNATDAAYIKAQSTKLGNAVLQDYGSRLV
                     GRSYIMVLDYQNVKTMQEAKINNMHGWQATAVGYLFKINYTEETQNALYDCWIYPEDS
                     PAVKAEKIKKFEQLEIPLEFVTKTSVPIVASQPNPDTQIGRFIKQKSDDVLLQELVQK
                     GYDETIYNLEKSHEDFMVKTTIHQVNPIRVKIGKKEGLKCDHRYFAYEYVYNEKTNTT
                     EPRFRGVIRATSKIVDNRQVATGEMPTSTFYQTAGRKLQTGYLVRQQNDMGLEVLAGF
                     EMGEVGGVYGRADFRLGRFVGIRSLFVFGEVGFQTKEYDILSLATEDVSFTHYGAGIA
                     KGFMLTRNVELRPYVGIGQESATSDAIEAANTGNYTTLYLRLGGNLALNLKHNIQLMG
                     GIGTYSFISNASNDNGDLGVTWDEVFPDRGGVSTLLGVKIMF"
     gene            complement(38603..40021)
                     /locus_tag="PK839_03260"
     CDS             complement(38603..40021)
                     /locus_tag="PK839_03260"
                     /inference="COORDINATES: protein motif:HMM:NF012470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HOG71788.1"
                     /translation="MKRRYLLPIWLILFIFPSLISAQNQKGAKYVFLFIGDGMGIQQV
                     NAAQVYADSVLHDTLGVTFMQFPVTTLATTYAANRYVTCSAAAGTALSTGYKTSIGTL
                     GLNANHTDTLYSIATRFRDNGRKVAILTSVSIDHATPAAFYAHAGSRSSYYQIALDLL
                     KSGYNFYGSGGFLEPNGSKHQGSTVASIYELGTRAGYGFTSSFTGVDSLKMLGVKSIV
                     YSTPNAAPEQTLQYEIDRKTSDISLADLTRKAIEVVDNPKGFFMMVEGGKIDWACHDN
                     DAATAIHEVLALSRAVNVALDFYSKHPSETLIIVTADHETGGMSLGNNANGYDLHLTA
                     LSLQKASADRLQAELYDLWSKNPKATFEDYLGLLEARIGLSENGAFALTADERNTLEA
                     AYKRLVNLNAGESKNAQKNAGLLAGDAISILNRKAGIGWTSHSHTGSPVPVFIMGRGS
                     SQLPGLLDNTDIPKLILKAAEL"
     gene            complement(40477..40564)
                     /locus_tag="PK839_03265"
     tRNA            complement(40477..40564)
                     /locus_tag="PK839_03265"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40528..40530),aa:Ser,seq:gga)
     gene            complement(40636..41676)
                     /locus_tag="PK839_03270"
     CDS             complement(40636..41676)
                     /locus_tag="PK839_03270"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002559173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I asparaginase"
                     /protein_id="HOG71789.1"
                     /translation="MKNRSSLSILIIYTGGTIGMKENPHTGALSPFNFDNIEEEVPEL
                     QKFGFNLQTISFDPPIDSSDITPDFWVKLAELISSNYEKYDGFVILHGTDTMSFTASA
                     LSFMLEGLAKPVVLTGSQLPIGMLRTDGKENLISAIEIAAAKRDGLPVVPEVTIFFEN
                     NLYRGNRTTKHNSEHFNAFRSDNYPLLAEAGITIKYNYPFIHYPRANSSLVVHTSMDS
                     SIGVIKLFPGITPQVVASALSAPGVKAVILETYGSGNALSQQWFIEMISKAVANGLTI
                     LNVTQCKAGSVNMNKYENGLILKNAGVASGFDITFEAAVAKLMYLLGKGFVGSALALE
                     FANPIAGEITLY"
     gene            complement(41663..42451)
                     /locus_tag="PK839_03275"
     CDS             complement(41663..42451)
                     /locus_tag="PK839_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016661797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOG71790.1"
                     /translation="MLLVDSHSHIFAEDFDYDREVVLDRAAQAGVKYHVLPNIDSTSV
                     PRLLETADRYSQCFPLMGLHPTSVNRNFSKELEVVGSYLSIRKFWGIGEIGIDLYWDK
                     TFVEEQKVAFQHQVQLAKKLGLPVAIHARNSFQEIFSILDEEADSTLSGVFHSFTGTL
                     EDYRHILDYQTFMVGIGGVVTFKNGGVDRVLPHINIEKIVLETDSPYLSPAPARGKRN
                     ESFNLTFIAQRVAGILEMPVHEVASLTSANAIKLFNLPINNEES"
     gene            complement(42550..43821)
                     /locus_tag="PK839_03280"
     CDS             complement(42550..43821)
                     /locus_tag="PK839_03280"
                     /inference="COORDINATES: protein motif:HMM:NF039309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOG71791.1"
                     /translation="MLKRAFFSVILALLAIFFVDYFVGISPVRTSKNSEEYSKDTLLA
                     KPILKYGIPVDSFYIEKGSLRYGQTIGNLLLDLNVDPEVVNSLTLVSADSFDPHKFKA
                     GNHYEVFRTRDTLAAVRYLVYEDSPVDFVIFDISDTLAIYPFKKEVTTVRRVDESAIT
                     SSLWETMKQKNINPMLALELSDVFAWTVDFFGLYPEDKFKVIYDELYVDSLPVGLGTI
                     HAAWFEHRGEKFYAFRYEQDSVASYWDEKGNSLRKSFLKAPLRFSRISSRFSGRRFHP
                     VLRIYRPHSGVDYAAPAGTPVMAIGDGVVIDKGYNESAGNYIKIRHNSVYTTGYNHLS
                     RFGKGIAEGVRVKQGDIIGYVGSTGYATGPHLDLRFWMNGKPVDPLKVKSPPVEPIRP
                     ENLEKFRQAITPIIYQLDSITPAGDSLRASR"
     gene            43998..45041
                     /gene="thiL"
                     /locus_tag="PK839_03285"
     CDS             43998..45041
                     /gene="thiL"
                     /locus_tag="PK839_03285"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008860802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HOG71792.1"
                     /translation="MSNQERKTELYELGEFRLIDRLTGSFGKLHETTIMGVGDDAAVV
                     DVGSKYMVITTDLLLEGIHFDLTYFPLKHLGYKAVVVNLSDVYAMNATPTQITVSIGV
                     SKRFGVEDLEELYAGVKMACERYGVDMVGGDTSSSMTGLTISITALGEVSKTDITYRR
                     GAKANDLICVTGNLGAAYMGLQLLEREKRVFESSPGAKPKLEGNSYILERMLKPEVRK
                     DTLQKLRDNKIKPTAMIDISDGLSSDLLHICKNSGVGCKIYADKIPIDSQTAQMAEEF
                     GIEPLVAALNGGEDYELLFTIPLEEFQNVSKIDGIATIGHITAESSGANLISPAGDEI
                     RLTAQGWNALAKE"
     gene            complement(45140..45523)
                     /gene="gcvH"
                     /locus_tag="PK839_03290"
     CDS             complement(45140..45523)
                     /gene="gcvH"
                     /locus_tag="PK839_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009126207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HOG71793.1"
                     /translation="MNIPSNLKYTKDHEWLKVEGNGEATVGITDFAQSQLGDIVFIEV
                     ETVGETLGHEEVFGTVEAVKTVSDMFMPVSGEVLEVNPKITDQPDVVNKDPYGEGWIV
                     KIKMTNPAEVNNLLTAEQYKSVVSE"
     gene            complement(45723..53066)
                     /gene="sprA"
                     /locus_tag="PK839_03295"
     CDS             complement(45723..53066)
                     /gene="sprA"
                     /locus_tag="PK839_03295"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HOG71794.1"
                     /translation="MFVFSCFLLFTPRVSIGGQVYEQTVNLQDTTRLPYPIKSENFLT
                     PATVRSPLFLNQPSNVTEVVEYDPTTKKYTVYQRVGGYNVGLPRVMSDEEYQNYRVEQ
                     SMREYWRQKQTGETVGKGDGILPRIQVGGESFDRIFGSNVIEIIPQGNAELVFGISSS
                     KTENPALPVDQRKNTTFDFQSKIQMNVAGKIGEKLKMEVNYNTEATFDFENNVKVEYN
                     GFEDEIIQRIEAGNVSLPLPGTLITGSQSLFGFKTEMKFGKLYVTSVVSKQNGQTQVV
                     EVKGGAQVRDFQIRADEYDANRHFFLSHYFRENYNRALANLPVINSGINITKIEVWVT
                     NKQANFENSRNLVAFADLGEADPNIFASSIFHQTVPGPADNSRNTLYELMTTTYSGIR
                     DIAEVNSTLLPLEGSGFSGGRDYEKIENARRLSSNEFTFNPVLGYISLNSPLYNDEML
                     AVAFEYTQDGKTYRVGELSTDGIAAPQALVVKLLKGTNLSPSLPTWKLMMKNIYSMNA
                     FQVSKNEFRLDVLYENDKTGTPLSYIGEDAIANIPLIRVLNLDNLNNQLDAIPDGVFD
                     FIEGVTINPAGGKIIFPVLEPFGRDLKAKFTNPAIADKYVFQELYDSTLTRARQMAEK
                     NKFILSGSYQSAGGSEIYLNAPNIPKGSVVVTAGGAKLQEDVQYIVDYNLGSVRILDQ
                     ALLESGTPIRISVESNALFSLQTKTLIGSHFDYRFSDNFNVGATIMNLTERPLTQKVS
                     FGNEAISNTIWGLNTSYQTDSRLLTRMVDFLPFIQTKEKSTVTFDAEFAHLIPGHSRA
                     IGKAGTVYLDDFEGSSTKLDQHYWTEWKLASTPQGQPALFPEASYSNDLRNGMNRAML
                     AWYFIDPLFLRNTSLTPSYIRNNKELQGSHFVREIFERELFPNRNTPQGQPTNISVLN
                     LSFYPDERGPYNYDDINVDPDGRLLNPQNRWGGVMRSIGTTDFETANIEYIEFWLMDP
                     FVYDSTMSGGDFYINLGNVSEDILRDGRKSFENGMPTTQAMEKVDTTVWGRVPRKQYL
                     PVGFDNDPANRKYQDIGLDGLSGEDLDGDGIPDEQSFFQNYLTQLRFRVSNEEVMAKS
                     DADPSNDNFIHFLNNQYDQSKATILDRYKYFNNTEGNSPVATGTTSQQAYSTPDVENL
                     SEGDITMSESEAYYQYRISLRPADMEVGRNYITNVISGEKDKLRNVPVKWYQFKIPIT
                     EYERVVGPIDDFKSIRFMRMFLRGFQDTMILRFATLELVKSEWRKYNYSLIQGQEGIP
                     SGDLPTTGFAISSVNIEENDKRTPVNYVLPPGVDRVIDPSQPQLTELNEQAMEYRVID
                     LADGDARAAFKNVNIDIRQYKKLKMDVHAEMVEGYPLSNGDVSAFIRIGTDYTDNYYE
                     YEIPLSLTPYGYYFNSRESDREMVWPRENLINIDLEKLVEVKVARNDAMRTPGSMVTQ
                     RTLYTVDDGDAQMKVVGNPNLSNVKVIMIGIRNPASGKGPTDDGMPKSVVAWFNEFRL
                     TNFNDQGGWAANARVSAKLADLGMINVAGSTVTPGFGSIEKKVNERSKETIYQYDINS
                     TLELGKFFPEKLNVRIPMFLGFSESISNPQYNPLDPDVTLSSSLNSYNPSERDSVKRI
                     VQDYSRHRSINFTNVKIDKTTEKPHFWNISNFSVSYSYSELFSRNIRTDHKIQRDIRG
                     ALTYNFNKQPKYIVPFKNVKWLNSKYFRLIKDFNFNLTPAQLSFRTDLDRSYFEQQFR
                     NVSNPSAVLLPTYSKDFLWNRNYALNWDITQSLKFDFTASNVARIDEPEGMVDRNRDP
                     KGYEHWRDSVWQNIKNFGRNTMYNHQFNLSYSLPLSKIPFLDWTNASARYTGIYRWQA
                     GPIMADTSRFDPGNTVQNSNTIQLNGQININTLFNKVGYLKRINQEFDSRGRGAAPKT
                     KTVKYEEGGVNIRAKYAKNINHKLKTDKVTVRVYDEKGNEVKVKSEVKSDTRVAIRAD
                     ADYSNCRVVVEGKVPDRPNPLKFAWEGTLRLMMSVKSLSLSYSETNGTLLPGYKPGTR
                     YLGIDSYNGFTAPGWEFIAGWQDPEIAWKAATNGWLTHDTTFNTPVVFTHNENLNFRS
                     SIEPVPGFRIELTANRTYSRNHNETYRANALGSYSSLSPLTTGNFSISVLTLGTTFEG
                     KNRDAKSRAFERMKSNRLVIAERLRDQRVANPGQGYDPMAPPDSLGFPVGYGPLSQDV
                     LRASFLAAYTNTSPQKVTLRDFPVIPMPNWQITYDGLAKIRPFKNLFKTFTLSHGYRS
                     AYTIGAFTTNLNYLPEDDGFSYVRNINGDFVPKREITNVSINEQFSPLLGLDMGWQNN
                     LTTRLEFKTSRSLAMSFANNQLTEIYGNEYIIGAGYRFENLPLVFQSEDGGKSAVKSD
                     LRITVDLSIRENTTILRKLVEDYSEIIAGQLVNTIKTSADYRINEQVTVRLFFDRTLN
                     APKVSRTYRTTNTSFGFSVRFELVK"
     gene            complement(53124..53708)
                     /gene="ruvA"
                     /locus_tag="PK839_03300"
     CDS             complement(53124..53708)
                     /gene="ruvA"
                     /locus_tag="PK839_03300"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007217309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HOG71795.1"
                     /translation="MYDYIVGKLAELTPTHAVVEVGGLGYHVNISLQTYSAINGMNEV
                     KLYLHQVIREDAHLLYGFFEAKEREIFRLLISVSGVGASTARLILSSLNADELQAAIL
                     TENINLIKQVKGIGLKTAQRIVVDLKDKVVGDDFASKQIMPAAHNTSRDEALSALVML
                     GFSKSATEKVLDQIIREGGELTVEGLIKSALKRL"
     gene            complement(53835..>54535)
                     /gene="pdxA"
                     /locus_tag="PK839_03305"
     CDS             complement(53835..>54535)
                     /gene="pdxA"
                     /locus_tag="PK839_03305"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005865827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HOG71796.1"
                     /translation="LITAPINKANIQSAKFSFPGHTEYLASSFNAKEFLMLMVGDLMR
                     VGVVTGHVPLSKVSSLITRDTVLGKIRLLNQTLVRDFAIRRPRIAVLGLNPHAGDAGL
                     LGGEEESAIIPAIKQAADEGIAALGPYPADGLFGSDGFTRFDAILAMYHDQGLTPFKA
                     ICFDSGVNYTAGLPVIRTSPDHGTAYDLAGQNLANPNSFRNALYLAIDIFNNRKLHDQ
                     LTTNPLSVSSSENG"
BASE COUNT        15153 a        12903 c        12088 g        14391 t
ORIGIN      
        1 cttttaccag cagtcagcca accacataca ttatggattg tgaagaaggt atgcagcccg
       61 attaatgtca tcaaccgagc ggatgggatt tggaggggtg aggtagcgtg ccaaaaactt
      121 gtagatttta acgcgaattt cacgtgcagc cttggtgtca atcctgtcga aagagtgtcc
      181 accgggcata tccttgaaaa tttcatactc aaacttctta ccttcggctt taagcgcttg
      241 aatgaggtgc tccacctcaa tcatataaac atcatcgtcg ttggtattgg tgtgtatcaa
      301 aagtggtgta ttcttgaatc ggtcaacctg ccaaacaggc gagcgacgct tgtactcggc
      361 aatgtcctcg cgggggctct tgtcgatgtg gtagggagcg gagtataact ctcggtagtc
      421 atcggtggag taaccaaacc gcatgatgag gtccgaaacc ggaaccccgg caaatgcgca
      481 ggcataatcg ttggggtggt caaaaatgtt gtgcagcgta atcatgccac catggctcca
      541 gccaatgatg ccaacccttt tctcatcaac aaaatcgtag ttttcaacca tatagttacg
      601 gctggcatac acgtcctcgt tctccaggcc gccatagtcg atattctccc aaaaactgcg
      661 tccatacccg gtgcttcccc tgtactctgc tgccactaca atgtaaccct gagccatcat
      721 ctcgcggatt atgtgggtat ggtaggtagt aaagtcagca tgaacacctc catgagggaa
      781 taccagcaag ggatactttt tagaagggtc gatatattta ggaataaaaa cgtaggccca
      841 gaacttaacc ggattagttg cccccatggc agtaggattt ttaacgtttg caggaggagg
      901 cccataaatg taaaccttgt caacaaaggc tacatcaccc accctattaa accaaagaac
      961 atcatccatt tgcttctgca tctcatcgag cctatggttt aggttgacat tctcctcctg
     1021 aagggcttcg agcctacggt tgatttcatc ggcctgctgc gagtatgccg ataaaaacaa
     1081 gcaaaacagc ggtagaacaa taaatttttt catctggttg taggttttgt ttgatataaa
     1141 gttaaaacaa aagtttgatg aatgctacca ccaaaagctt aaccgccagt cttaaccggc
     1201 tataaggaaa tgcaattttc taatcgggta ccaccgattt taccaccggt acccttctac
     1261 tgaaaagcaa ggcggaaata gcacaaagtc cccctgcaac agccatggtt ggctgaattc
     1321 caaccgaagg gctaatggcg cctagcaaca ggctccccaa aggtggaaat ccgttgaacg
     1381 aaagactgta gagcgacagg atacgacccc gcttatcgtc gtcaacaata tactgcagga
     1441 gcgtgttggt tgcagcaagc tgcacaatca tcccaaaacc agtaaagaag agtaggccaa
     1501 ccgaaagcgt agcataggat gacaatgaaa aagctgccag cgatgttcca aatgttagcg
     1561 agctgtagag gataatattt ggaatgacct ttatgctctt aagcgacgcc agcaggatgg
     1621 ccccggtgag cgcaccggca cctaccgccc cggtaagcag tccaagcatt tgcgagttac
     1681 cgtgcaggat atcacgtgca aatacaggca taaaagtttg aaagggaaat cctagaaacc
     1741 ctaccacaac caggagagag agcacatacc tgataggcag gaaattatag gagtaggaca
     1801 atccctcctt caactccttg aatatggatg aattatgtgg ttttctttca acttgggcca
     1861 cccggatatg aagtagggag gcaattacgg ccataaagct gattccatta accagaaagc
     1921 aaaaaccctc tccaatccat gcaaccagaa cacccccaat ggcaggacct atgaaacgtg
     1981 ccgagttaac caacgttgag tttaaagcta tggcgttttg aagcaaatcc ttatcgccta
     2041 ccatttcaag cataaacgaa tgcctgaacg gagcatcgaa agctattgcg attccgttca
     2101 aaatggaggt tacaaaaatt tgccaaacct gtatggtatg tgtaaagtag agtgtagcca
     2161 gggttaatgc aacaatcatg gaaataccct gagtaataac aagtactttg cgcctgttca
     2221 acctatcagc gtaaacgccg gcaaagggca tgagaaccag cgacggtatt tgaccggcaa
     2281 aactaacagc acccagcaaa aaaggagaat ttgtcagcct ataaaccaac catcccaacg
     2341 ctaagccctg gatgaaggtt cccatgagag aaactgcctg gccgtaaaag aaaatcctaa
     2401 agttgggact ttttaacgct cgaaaagcaa acttgatttt gcttaggtag gatgcaggat
     2461 taagatgaaa agtcattatt atttagatgg gtaggtattt ggttatgcaa aaatacaaaa
     2521 tatatgctga cgtcgtgtat tgcccatcac gcttgattga cctcccaagg gtaggaaact
     2581 taatgggtaa ggcatgttag tcattaaagt taaaaaatgt tactccccta atcttttgtt
     2641 aattctgatt tagctgtagc gcaaccatcc gtaatgtgat aatagctaca tttgcagcaa
     2701 gtgaaaaaaa acgtatttct gtgaagaaag taaaaacaat tgccctggtt gcgcatgatc
     2761 accgtaagcg cgacctggtt gaatgggtcg aatggaactg gcaggaacta cttaagcaca
     2821 agctcatctg cacgggaaca accggtcgtc ttattgagga atcgttacag aaaaagaaga
     2881 gtgacgtagc agaccacatc cagctaaccc gccttaaatc aggcccgctg ggtggggatc
     2941 agcagctggg tgctctaata gccgagggca aaattgacat tctgatattc ttctgggacc
     3001 ccatggaagc ccagccccac gatgtggatg ttaaagccct gctccggatt gcaaccgttt
     3061 acaatattcc cacagcaacc aaccgctcaa cagcagattt tcttatcagc tcccccttga
     3121 ttgatgagga ttacaaccct attctgtttg actacagcca ctacatgaat cgaaaggtat
     3181 tttaacaagt cctttctact tcaattttga ggttaagcag cgcctgaatc tggctcgaaa
     3241 tggatttctt gacagtttca gggcatacca attacaaccg gcttaatgca ggagttgcac
     3301 tagcactgat aacttctact gtggtcttgc tatgggctta tgtgctctcc acattaaggc
     3361 ctaatatctt tgatcatcgg gcaacaaggt gttcaatttc agcgccttat cgcactactc
     3421 atcagaacat aacaaatcat aaaagctgac tagacaacag ccactcatag aacattatca
     3481 ggcataaata caaaatctat gagaatgtgt ttactaaaat tttgaattgt gatttaaata
     3541 ttgctaaatt tcaccaaaat ttcataaaca aaaacgctgg ttatgaacca caataaaggt
     3601 aaatggtgca gaacaggcag catggttttg cttacagcac tacttacagg atgctacgat
     3661 ttttcaaatt ttgaaaattt caaggttgaa cctgtaaacc cctccatagt tgctccggtg
     3721 gttaactcca ctataacttt taaggagctg gccgaaactg aagacgccaa taccattgtg
     3781 attgttaaac caggcgatac caggttttac ctctccttcc gcgataccat ggaaattgcc
     3841 aatgccagtg gtgagttttc aattccacca tttgcctact ccaaaaccta tcaacccgat
     3901 gcaggtgaaa ttcctccggt tgcacctcca gctggcgaaa gtattacctt cagccccaaa
     3961 accttcaccc aaacctggca acctgacgcc gattctgagc taaaactcat caagctatct
     4021 cagggaagta tgaacattac cgtaaccaac aacttcggaa acatcattag cggcagcatc
     4081 acgttaaact ttctactaac ccctcagaat caaccctaca ctatcaacat tcccaatatc
     4141 aatcccgctt caagtcaagc catttcgcta acaaacctgg cgggctacag ctttaacctt
     4201 ttcgacggca ccagctacaa cacccttaca tttaccgcta ccatcaccct cacatctatg
     4261 ggcaattcta taaaccctgg cgataacatc aatatcgaca tttcggcaaa caatctcgac
     4321 ttcaccgaaa tttacggcaa ggtcaataaa accatcaacc tttccgacca atcttttccg
     4381 ttggatatat tccgttccac ctacattgcc gaccagcacc tggccgagcc cagccttatg
     4441 tttaagttcg ccaacagcta cgggattccc ctgtcgctta acgttaacca gctggtagcc
     4501 agcaacgacc aaactggatc catgaacgtg gttaacgaaa ctgttcctcc cgatgggtca
     4561 ctactcattg gtgcacccaa ttcgttaaac tatgttggga gcccatccca ggaatttgca
     4621 atggactccc taaagctgga ctatcaaaac tccaacattg aggatgtttt cgactttgcc
     4681 cctaacaatt ttacccttaa atcgggaatt acccttggcg atgcctcaac cagccacgac
     4741 tactttgtta gaaaaaactc caaccttacc cttttcagta atgtagaagt tccgctggtt
     4801 ggctgggttg agactaacgg cattcgcgat accgtagaga tagaccttcc ggatattgag
     4861 aacgatttga acatggaaga tgacgacgag ttgagcctaa ctctgaagtt caggtttaac
     4921 aacggtattc ccctgaatgt ttacttccag gcttacttta ttgacgatta cgaccatgaa
     4981 cttaccaggc tatacacaaa tgtggcagaa gagcagctta ttgaaagcgc tgcagtaaac
     5041 ccatcaaccg gaaaagtcac aactccaaac gtaaaaattt ctgaaatttc gattgacaga
     5101 aataggtata aacaaatgca gggtgccacc aagctcgttc ttcaggtacg gtttaaaacg
     5161 ggggggagca ctcatcaaaa catagttgtt gaaagcacca actccataga tattcagatg
     5221 agcatcaagg caaaaggaac ggttagattc gattcctagt gttaggagta tgccgataaa
     5281 tagctataca gctacaaatg aagcgcccac gatttgattt aacgcaaccg tgaataagtt
     5341 aaacttgaat atttgccttt ggcaagcctt atgaactcca aatgctggtg aaaactaaaa
     5401 agtgaaagca caacttattg tcaactaata atattaacat acttttcgga tgaaacgtct
     5461 gtctttacta gcactaatac tcaccgtcgg catttatgcc aaggcccaat cggagctaac
     5521 gctaccattc atgcccggtg tttttcagtc atcgttcgta aatcctaccg ttatccctga
     5581 acatacattc agcatgggct ttagtattta tacccaggta gttacaaatg ggttcgtgcc
     5641 taacaacgtg cttatattca gggatgacac catgcacttt gaccccaacc tgctgctcga
     5701 cgatctcagg gataaaaacc tattctacat gggtgagaat acggacatct tccatattcg
     5761 tacaaaaatt cttaatgggt actactggtt tgccattagg caaagaatgg aatcgtcgtt
     5821 tttctaccca tacgatcttt tccgcatggt tattgagggt aatgaaccct acatcggcca
     5881 aaccctcgac ttctcaaacc tgaagatttt cacctcccta ttcaacgagt actcctttgg
     5941 catgacgaag gagtaccccc actggatttt tggtggcagg cttagcctgc tacaaggcct
     6001 aagcaacatt aattttaacc ccacaaggct tagcattaag gtggaagatg aaatgtacgg
     6061 ccagctggcc aacgcagatg cccagctgag aacatcgggc attcccaaag atggcaatgg
     6121 cgatcccagc ttcgatcatg ttgaagaacc tgagtggata accgattact ttaccaactt
     6181 caagaacaaa ggatttgcgc ttagcggggg tgctacctac aggtatgacg accggacacg
     6241 aatttcgctt tcattccgcg acgttgggtt tatcacatgg aaggacagca ttgaaaccta
     6301 cacccttaac ggaaacacca acttcaccgg gctggatatc atgagcgacc tcctttacgg
     6361 caacgatgtt gaggtggact ccatttttga aaatatcctc gatgaggatt ttcaccgtga
     6421 caccatatac aacaagtaca ccacatggct caacccgaag tttaacctta cggctagcta
     6481 cgacctggct cgtagaacaa ctgttggatt ttccttttcc ggagtagtta accagaaact
     6541 ctacaccgca ttaaccctaa gcctttcgca ggggattggc cggtttttta gcattgttgg
     6601 cacggcatcc atcaaccaga gatcttttac caatctggga cttggcctaa tgctgaagcc
     6661 gggcccactg caaatttttg tggttgccga caacctttac gccgcccata acccgctgac
     6721 tactacaaac ttcaacatta gggtaggtgt gaatcttgta ttcggtagaa taaaccaacc
     6781 cgaaggatta cccttccgtt aatgcaacac aaattgatga atttccgact taatccgttt
     6841 cacacatgag agtatcagga attaagaata aaatagtagc actgcttctt ggaagcatgg
     6901 tagcgttaag cctgcaggcc cagacctctt tcacccctat caacctggga gatagggtaa
     6961 attctgaata ccctgaaatt aaccctatac tgcatcccga tggtaaaact ctgtattttt
     7021 cgagggccaa caacccagag aatacatggg gaggcgttaa cagccaggat atatggtact
     7081 ccgttttagg agacgacgga acatggagcc aggcccaaag gctaccccgt gctgttaata
     7141 ttggtaggta caacgccctg tttgccatcc tgaacaacgg gaaaactttt ctcattaatg
     7201 gccattactc tccaaatgga aagtactgga ttgaaagggg actttcgact gttgagcaag
     7261 ttgagaatga aaattggggg aaacctcaaa gtattgaggt aaagggttat agcaacaaga
     7321 ataggggaaa aaccacaact atattcgtaa gccccgacga gcaaaaactc ttcatggcat
     7381 tcagtaaaaa agtaaatggt aaaaaggcca gcatttacct gtccgagcga aaaggcgaca
     7441 ggcgttactc caaacctaaa aaaataaatt ttctaggaaa taacaagtgc gaaagcatgg
     7501 aggcaccttt ccttacctcc gatggtaacg ccctgtacta cagctgcaag attaatggtg
     7561 ataacaacct gtacatgagc caccgcaccg atgataccta ccgcagctgg tcggagccca
     7621 taccgctaaa cgatacaatt aacactccgg gttgggaatg ctactaccac ctgaacagta
     7681 agggtagctg ggcatacttc tgctccaata caaactccct cgggaagtca gatatcttca
     7741 gggttaaaat atttgaggaa aacccgttcg taaggcttca cgggttggtt ctcaacaagg
     7801 ctgatcaaaa actaatgctg gccgatacaa gctacatctt aaaagtgaat ggctctgaac
     7861 cagaaaagct aaaggttgat agggcagcgg catcctacga acttctgctc ccatttggcc
     7921 aaagctacca gcttcaaccc gaaatgaaaa actggattgg aatacccgaa ttatacgatg
     7981 ccacgggcat taaggagttc actgatgttg agctaaacct ttactttgag ccaattccct
     8041 acgttaaaat atttggtaag gtaatcaata ccagaaccaa cctacccatt tctcccgaaa
     8101 taggcttcaa ggtgcttata aacgggctag aaaatgattc ggtgaagtac attagggata
     8161 tttcaggcta ccaggcactg ctgccactag gccagaagta tgtggtttcg cttaacctcc
     8221 caaactacag gtcggaagcg gatacggttg atgtaagcaa tatatccttc tacaccgaga
     8281 aacaggtaga ttttcatgta acctcagcac catgggtcga agtttacggt attgccatgg
     8341 ataacgtttc ctttaccccc attcccggct cctcttcacc caagctactg gttaacggtt
     8401 accctgccga tagtatcgct attgatccgt ttaccggaga gtttaccatt aaactaccct
     8461 acgggataaa acacaacctg gcaatcagct caaaggatta caacccgttg gataacgtgg
     8521 ttgatttaac cggctacgtt gagtttgccc gtataaatca cgacgtatat gctgaacgta
     8581 aggatgccaa catggcaacg ctttacggta aggttatcaa tctaaaaacc ggacaaccag
     8641 ttgaatcgag tattcctgta aaactcaagg tgaatggagc tgaaaccagg gcctttattt
     8701 acgattctac cactgctgcc tacaccttga aacttcctgt aggcgtttca tacgacattc
     8761 ttccatcggt aaaaaacttt tacaacaagt acgagcaggt tgacctgacc aagactaaaa
     8821 aaggcaccaa ggttcccaag aacttctaca ttacacctat tgaggttgga caggcggtta
     8881 acattgactt tatctacttc gaaacgggca aatcaaccct gaaaccccag tcgttccgat
     8941 cgcttaatgc cctggtggat ttcctcaacg agtaccccaa tgtaatagtt gaaattggcg
     9001 ggcatacgga caacacgggt tcggcagcta taaacgaaaa aatatccaac cttagggcaa
     9061 aggctgtagc tgactatgtg gtatcgatgg gcatccccgc ctcgcggata acttcaaaag
     9121 gttatagctt caacaaacct aaagccagca acgcaacagc aaccggcagg gctcagaaca
     9181 gaagggttga gtttaccatt gtaggaatat aatacaatct gatgaaacga acgagtgtgg
     9241 tcatgtgtaa gaacatactc gttcttttta ttgtttaaag caatacccgg taccaatcaa
     9301 taatgttggc tgtgtcaacc ttttactgcc tattcatctc ccgcaatgaa accaaaaggc
     9361 atttttacgc caacctgttg actaccggta gtagtttttc acacagaagt tgaaacatgt
     9421 ttgccctaaa gctaaaattg ctgctaagct gttttgtagc cgtgctggcg ctggttaacc
     9481 ccatccaaaa ggtattcgta atgacaagcc ttcaggatca gttcagcgaa gccgatacca
     9541 aaatggttgc tcttaagtcg agcctaacag cattgctcat tctgttgttc ttcctcctgc
     9601 taggagaggt tgttttcaac tacgttttcc atgtcgagct gtacgccttc cagataacct
     9661 gcggcgtggt tttgttttac aatggtatta ccggattgca aaagggcatt tttataaagg
     9721 ttgaccagaa catcagcata cacgatataa cggccgttcc cattgccata cccatgattg
     9781 ccggaccagc aaccattaca gctgctgtta cctttccagc ccactatgga cacttcaaca
     9841 ccattgtttc cattgttgct gcccttgcca ttaacctcct cttcatgctc tacgccaagc
     9901 atatagcaag attcttaaac cgtttcaacc ttatgaatcc actggttaga atttttggac
     9961 ttatagtggc cactattggg gttcaaatga cgctaaatgg aatttggggg tttgtggttt
    10021 cagttgctgg acacgtgtaa cttcgagcta ataatcacca caaaaaatcc tcatacattg
    10081 ctatatttgc agaacataag aaacattatc ggtggaacaa aaaaatttta tagacattga
    10141 tcaggttatt gcctcaaaaa gccctacgct tcaaaaaagc ctcccccgtt tcattgtcaa
    10201 ctacctaaag cgtatagttc atcaggacga aataaatgag attctggcca agcatgggca
    10261 taagcaggga gctgagtttg tggaggcagt tctcagcaca atgggggtaa actactcggt
    10321 tgaggggttg gaaaacctgc aggataacca acgctacctt tttgcatcga accacccgct
    10381 cggtgggctc gatggcatgg tgcttattca cctgctggca aaaaggtttg acgaggtgaa
    10441 gtttcccgta aacgacctgc tcatgcacat taaacccctc tccagcgtat tcataccagt
    10501 taataagctt ggaacgcaga gcgcttcaaa cgccattcag cttgaggagg cttacagctc
    10561 atcggcacaa attctatact ttcctgccgg cctttgctca cgcaagcaaa agggtagaat
    10621 tgaggacctg gaatggaaaa aaaattttat tgttaaagcc attaaacatg agcgggacgt
    10681 ggtgccggta ttttttgacg ggcgtaactc cacctttttt tacaacctag ccaggttacg
    10741 ggctaagctg aggattaaaa ccaacatcga gatgctctac ctttccgatg aaatgttcag
    10801 gcaaaaagga aagagcatta cggttaaaat aggtaagcct atcccattca gcacctttga
    10861 caacaccagg aatgcccatg aatgggctca atgggttaag catgtggttt accagatgaa
    10921 gcccgcttca taaactgtca aattgatgca gtaaaaacca acccagtaaa ctccaattct
    10981 agaagatact agccatgcag gaaataatcg aaccggttga ccggaaccta ctaatggcag
    11041 aactcacccc cgagaagttc gttaggaaca ccaacaatgc ggaaaatgag ctctacctct
    11101 ttaccgccca tgattcgccc aacctgatgc gcgaggtggg caggctaagg gaatttactt
    11161 ttcgctctgc cggtgggggc accggagagg aggttgatat agatgagtac gacatctgcg
    11221 ataactgcta caagcagctt attgtttggg atccaaagga aagggaaata cttggaggat
    11281 acaggttcca cagcgttgac ccctcaaagg gcgatgaaat taacctctcc accaagcacc
    11341 tgtttaattt ctctgaaaaa tttgtaagag agtatatccc taaaatgatt gagctgggac
    11401 gctcttttgt tcagcctaag taccagtcgc tcagcagggg tggcaaagga ctttacgccc
    11461 ttgacaacct gtgggatggg ctgggcgccc ttatcgtgct taacccccac atcaggtact
    11521 tcttcggcaa ggttaccatg taccgctcct acaacgtaga ggctagagac acactcctct
    11581 acttctttga aaaatacttt aaggacaacg aacagctcgt tacaaccata catccaattg
    11641 aggtgaacat ggatcgggat aaaatggaaa ggctattcac cggaaaaaac tacacggagg
    11701 attacaaaat tctatcgcac aagataagag agcttggcga gaaaattccc cccctcatta
    11761 acgcctacat gaaccttagc ccatccatga aagtttttgg cacggttata aacccctact
    11821 tcggaaatgt tgaagaaaca gcaattctta taactattcc ggatatttac cagcataaaa
    11881 tggaaaggca caccggcaca ttcgtgagga agctggccgg taaataccta agaccctacc
    11941 gaaaggaaag ctgaccgcag gggagcagaa aaccctgctc agccgggttc aaccagcaaa
    12001 agatttacca tgacagcacg gacagagaaa gttcactgtt actggtatgc cctgttgtta
    12061 cggccctcaa taaagttgat aaacgagttg ttcaccaccc tatttccacc cggagtaggg
    12121 tagttgccgg tgaagtacca atcgcccaaa tcgttggggc aggccaggtg aaggttttcg
    12181 attgactggt acacaatttt aaccttagcc ctcatatcct ttggggttaa tagctgggcg
    12241 attttttcgg caagttgatc gtccgtaaac gggctgtaaa tctccttgac gtaattcaca
    12301 atttcctctt taggcagata ttgctgttcc ttacacttcc ggtaaacctt gttgatgatg
    12361 gcctcctgac ctgtatcgtg cagcaatcca acagcggcct tgaaagctac aaaatcgcta
    12421 agcttggcca tatcgatgcc gtagcaatcg ggatagcgaa tttgaggtga ggaggacacc
    12481 accacaacgc ttacgggatt tagcctgtcg agtattctta gaatgctctc ctttagggtg
    12541 gttcccctta ctattgaatc gtcaatcacc accaggctgt ctatccccgg cctgatggcg
    12601 ccataggtaa tgtcgtacac atgcccaaca agatcctccc tccccttatc ctgggaaata
    12661 aacgtacgaa gcttaacatc cttaacggca attttttcga ctcgtaaccg aatggcgatt
    12721 acctcatcca aatcgctctc ggtcaatgag ctgctctttt ccaaaatcat ccgcttcttc
    12781 tccaccttca ggtaatcctc caccccctta accataccgt agaatgccgt ttcagcggtg
    12841 ttgggaatat aagaaaacac agtattggaa tggtcataat taatggcctt caggattgcc
    12901 ggggttagca gttcgcccaa ctttttacgc tcctggtata tatccctgtc tgagccccgt
    12961 gaaaagtaaa tcctctcgaa tgagcatgac cttcgttgct ggggtaccct tatctcctca
    13021 tgggtgatag ttccgttacg cttgataatc aagccatagc ctgggttaac ctccttaaca
    13081 tcatcggttt taacattcat aaccgtttgt atggccgaac gttcagaagc cacaacggca
    13141 aattcgtcgt ttaaatagta gaacgctgga cgaatgcccc atgggtcgcg aattacaaag
    13201 gcatcgccat gaccaataag accggcaatg gcatacccac cgtcaaaatc ctttgttgct
    13261 gcctgaagta tcttgccaat gttaatgttc tcggcaatta gcttgctaat ctccatgttg
    13321 gagtacccct cattcttata ctgtcgaaaa acctgctggt tttcctcatc cagaaagtgg
    13381 ccaatctttt ccagaacagt tacggtgtcg gagtaatcac gtgggtgctg ccccaagctt
    13441 accagcttgt taaagagctc atccacgttg gtcatgttaa agttcccggc cagaaccaga
    13501 gtacgtgatt tccagttgtt gtcgcgcatt acgggatgaa cgtaatctat ggagttgttt
    13561 ccaaaagtac cataccttaa gtggcccaag tatatttcac cggcaaaggg aatattttcc
    13621 ttagcccagg ccgaatcacc ggctagttca ggtctatgcc cgtactggtt aatgctccta
    13681 ttaacggcat caaaaatatc cttaatggaa gaatccgaat tagaacgctc cctgtcgatg
    13741 taccgcttcc ccggttccaa atcgaactta agagaggtta gccctgctcc atcctgtcca
    13801 cggttgtgct gcttttccat tagaaggtac agcttctgaa gcccatactg ccatgtgccg
    13861 tacttaaggt ggtagtactc cataggttta agcagcctaa taagcgcaat accacattcg
    13921 tgtttaatct gctcgctcat tttatgtttc ctgtatgata aaaaaaggta gattaatcta
    13981 cccgtttaca atattccttt tttattacga gaaagggttt ccaaaacctt cctggcaata
    14041 gactgggagt tgagcccata cttctgcaac agctcggctg gagtaccaga ctctccaaag
    14101 caatcgggca ttccaacaat ttccattggc gttgggcaat atttcccaag aacttcggca
    14161 acagctccac caagtccacc gtttacctgg tgctcctcaa cagtaaccac aaaaccggta
    14221 tggtgggcca tatctacaag tagtggtata tctaaaggct taattgtgtg aatattaacc
    14281 accatagcat taaccccttg cccggccaga atatgggcag cctcgattga ctcccaaacc
    14341 tcatgcccgg tagctacaat tgcaacgtcg tcgccattca taagaacctg ccccttcccc
    14401 accactagct tctgatcctc atcggtaaaa ttggggacgg gctcacgacc gaagcgaatg
    14461 taaacaggcc cattcacctg ctgcatagca gcaatggtag cagccctggc ctgattggca
    14521 tcgcacggag aaataagggt catatttggt aatgccctga ggtaggctat atcctccagc
    14581 gcctgatggg tagcaccatc ggggccaacc gatattccgg catgagcacc acctataata
    14641 accttaacgt tgttgtagca tattgaaatt cgtagctggt cggcagcgcg tagtgcggca
    14701 aaaacgccat aggaggaaaa tacaggaagt tttccggcca acgccaaacc tgctgccact
    14761 gcggtaatgt tctgttcggc aataccaaag gaaaaaaaac gttcagggaa cctacgcttg
    14821 aaaccatcca acccaaccga ggcggttatg tcggcaccca gcaccaccac gttgggattg
    14881 agctccccaa tatcaaccac tccctcacca aagcccgttc gggtagcctt atcgccttta
    14941 ttcctgaact gttccatttt gtagagataa gtagtgagcg tgtagctcat caagaaactt
    15001 ttccgcctcg gtagggcttg gtgccttccc atgccagcgg taatcgtcct gtatgctttt
    15061 aacgccatat cccataagcg ttctggcaat aattaccgta ggtttcacat caccatgctc
    15121 tgcaacttca aagatttcaa ccagctgggc aatatcattt ccatcacact ccagcaccct
    15181 ccagccaaaa gattcccatt ttgctctaag gggctcaagc cccataacct ccgatgttcg
    15241 gccatcaatc tggcagtcgt ttctatcaac cagggcaatc aggttattga gtttatgatg
    15301 ggcagcgctc atggctgcct cccagattga tccctcctgt agttccccat cgccatggat
    15361 ggaaaagacc cgccatgggg ctctatcaat tttagcggcc agagctaagc ctactgctac
    15421 cgaaagtccc tgaccaagcg aacctgctga aagttcaagt ccgggcaatc catgatccct
    15481 tccgggatgt ccttgtaggc gcgagcccag ctttcgtaag gtgagaagtt cctccctggg
    15541 aaagtatccc gagttggcca aagcggcgta gagcaccggg gcagcatgtc ctatagatag
    15601 tatcagcctg tcccgctcgc tccaaccggg ctgaagcgga cggtgatgca gaacttgcca
    15661 gtagagcact gtaaaaatat cggcaagccc taacgaggct ccgagatggc ctgaaccagc
    15721 tgaggctaaa ctccgaacga catcctcccg aagttgtttc gataccttta gaagctggtg
    15781 caagtttgcc tggaccataa cactactcca gtggtggaaa gttaatccat tgcggaatta
    15841 taaatgcttt aggataatct acaagaagct ggtggtaaag tttttgagcg ctctcccgag
    15901 tacggaaatc acccaccgaa attttccagt agggattgtc gtagctttgg taaactctaa
    15961 tatcagggta cttttccata aaagcattca tcactgcctc ggagttgcga cgagcgtttt
    16021 gccccagctc caggaatatg cgaatacgaa aaccgttcat ccccggattc ttagcattct
    16081 gaagtatgtg cagctccaac aggtttgaaa ccactggatc ctgggtaatt ttaacctttc
    16141 cctgctcatt gccttcgtgc tgattttgca caattccact taccccctgt ccgtgaacat
    16201 gctcagcggc aaatacacaa atcagaaaaa gcgagttaaa gagaagtgct ggtaaactac
    16261 gcatcggaat ataattgcac cacaaaagta gcaaaagtta gcctaattgc caaccgtcga
    16321 attgcggacg gaaattgtgt cggtaataat aaacccgttt tggtacttgt tgatgagatt
    16381 gttcagaggc tgccggggga ttttaatttt ttttacaatg gggaagctcg accccttaac
    16441 aaaaccctca acggttatca ttgtcagaat cttttcgccg gaaatcatca actttagggc
    16501 atcagcagaa gaatttaatt ttacttcaac agggatagag tagatttggc ttgagttggg
    16561 tggaatttca attctatttt taactctaac agttcccagt tccctttgtt ttatccacga
    16621 tttaaactca acctctttta cagttatcct tttggaattt ggattctcaa tccctactgc
    16681 taaattcagc ttaacaacgt cattctttaa cccgttatac tcaactttgt ttatcccgtt
    16741 gattttaatg tcctgaaatt tgggtgcgca tccggacatt aataccatga agataggcac
    16801 aaaagcagca agaaaccttt taaccttcat ctattgtaga attttaattt aattagcaat
    16861 aactgttcca tgctgcttat aaaaagaaga aaaagccatc gaacaagcta gccgatggcc
    16921 tcaatattgt aaaacgttta taaattctac tactcatttt ccatagcagc cacaacctca
    16981 tcggtcattt ccaggttatg gtaaacattt tgaacatcgt cgttatcctc aagctcgtct
    17041 atcagcttaa ggaatctaag cgatgaatcc ttgtcaagtg ttttatagtc attggggata
    17101 cgctgtaggc ttgcattttc aacttcaaca ttcagctcct ccagcttttt gttcatccgg
    17161 ccaaagtctt ccatttcggt agttactacg tagaaatctc cattgtcatc gatactttgg
    17221 gcgccggcat ctatcaggtc aagctccacc tcgtcgagct tcagcttatc cttggcaata
    17281 gtaaaaatgc ccttgcggtc gaaaagaaac gataatgagc cgttagttcc aaggcttcca
    17341 ccacgcttgg tgagaatgga acgaatagcg ctaaccgttc gattgttatt atcggtaaga
    17401 cattctataa ataatgctat tccacccgga ccataaccct caaaggtaag ctcatgaaag
    17461 ttttcgggat ccttctcagc cttggatatt gcccgcataa tgttatcctt gggcatgttc
    17521 accccctttg cgttgtttat agcaaggcgc agccgggggt taccatcagg atcggagcca
    17581 ctttccttga ccgccacggt aatttccttg attattcggg aaaactcctt ggagcgtttg
    17641 gcatcaagcg ctcccttttt tcttttaatg gttgaccatt tattatgtcc tgacataaag
    17701 tatttgttta gcttattcaa ttataccatg caaaagtaac cgcttgtttt ttgctatcaa
    17761 aaaaaatcgg aagttttcac actggttcag gtaaaaatat aggtttagga cttgcaggtc
    17821 taataaaagt atatctgaat ttttagcatt tcattacatt tgtaaaaaaa aagaatggac
    17881 tttaacggcg aggagcccaa catttttgaa ggtatatcaa ccgcaggaac aggcaacggc
    17941 gcaaaggttt acctggaaac atacggttgc cagatgaatg taaacgatag cgaggtagtt
    18001 gcctccattc tgctggcaaa cggatactcc ataacgcagg agataaacga agcggacatc
    18061 atacttatca atacctgttc aattagggaa aatgccgaaa ccagggtttt tggccgtatt
    18121 gacctgtttg gacaggtaaa gaaaaataaa ccatcggtaa ttataggcgt tttaggatgt
    18181 atggccgagc ggcttaagga tgagctactg gagaagaaga aaattgtgga tttggtggtt
    18241 ggcccagacg cctataggga gctgccaact ctgctacgag cggctgaaag tggacaaaag
    18301 ggggtaaaca ttatgctctc agccgatgaa acctatgcag acataagccc ggtgaggctc
    18361 gacaagtatg gcgtatcagc ctttgtatcc ataatgcggg ggtgcaacaa catgtgctcc
    18421 tactgtgtgg ttccctacac ccgaggagca gaacgcagcc gagatcccca aaccattatc
    18481 catgaggtag aagagctggt taggaacggg taccgggagg ttacgctgct tggtcaaaat
    18541 gtcaactcat attcctggag cgaatcgaag gggatggtaa agcacaccgt taactttgcc
    18601 gaccttctgg agatggttgc cctggtgagt cctttcctaa gagtgcggtt ttccacttcc
    18661 catccaaaag acctgaccaa tgatgtgctt tacaccatgg caatgtacga gaatatttgt
    18721 aaccacatcc atctacccgt gcagtcaggc tcagaccgca ttctccagct gatgaatcgc
    18781 aagtactccc gcaacagcta cctaaacaga atacaggccg tgcgggaaat aattcccgac
    18841 tgtgctatat caaccgatat tattgcaggt ttttgcactg aaaccgagct cgatcacgac
    18901 ctcaccctaa gcctgatgag ggaggttggg tttgactttg cctacatgtt caagtactcc
    18961 caacgcccaa atacaaaagc ggccaggcat ttagttgatg atgtacccga tgaaattaaa
    19021 tcaaggcgct taaccgaaat catcgagcta caaggtaagc tgtcggaaga aagtaagaaa
    19081 aaggatgttg ggaaacgatt cgaggtgctg gttgaaggca cctcaaaaaa atcggacgat
    19141 ttctatgtgg gtcgcacaac acacaataaa gtggtggtgt ttccaaaaga agcaagtagt
    19201 attggcgatt tcgtgctggt tgaggttacc agctgcacgc cagctaccct aattgggaag
    19261 attgttgacg agtagtagta aaaaaggccg tataaaacgg ccttttttgg ttgaaataat
    19321 actctaacac tacatcagct tgttctgaaa ttcgctcttt aaatttcccc cctcatcaaa
    19381 ccactcggtt agcccgtaaa agtaatttgt actcccctcg ggtttgaact tcacaaccaa
    19441 cttaagattt tttgttaagc gggtaagatt tgtagccaca gccattctta ctctatccct
    19501 atccgactcg tcgataccta aacgctccat tgcctcaacg gtgagagagt ttgccagcat
    19561 caagttttgc ttgctacgaa gaatatcctg aattagatca atccatttta ccttgtcggg
    19621 accagcaccc gacttaatct tctttcgtac gacactttcc tttcggggcg atttcttagc
    19681 ggcaactttt gatttcaaat ttgcctttct cccccttttt gtagcagcca gcttggtttc
    19741 ttcggaggta gcagcattag ccttactttt ccttgtcttt ctaatggctt ttactttagg
    19801 ggcagcactc ttagcaacgg ctttcgcatt ccggcctttt cgaacttttc taccatcaac
    19861 cttggcctcg ggtgtaactg cttcaccggc tgctgggact gattcaacca attttttcct
    19921 ccctcttctt gctggagcag caacccggga ggcgggttcc gaaacttctt cttgtgcccg
    19981 atcaatcttt tttagaattg attttaaatt ttcaattcta cgttgagttc tgtcaatctc
    20041 ggtttggtag agatcaatca aatcgttaat ctctcctggg gttaaattta ttttactcat
    20101 cttttagtgc tattaaaaat taactgatta gttgaaaatt tcattctaaa actgttacca
    20161 ttactaaata aaataaaggt gaaaacaaaa aaatatctat aaaaagaaaa taataatttt
    20221 tataatttaa ctttttatca acaaatcaac tagttaataa gtttcaaaaa tagataaaaa
    20281 aatattatat aagtacaact tgtgtgattt tttttatttt aattatcaat gtttaattaa
    20341 atccctaatg ttgatgagct ctctcatcgt tttcaaaata accatttatg aaaggcgtat
    20401 gtagtgaaat tgatttacaa atatcaacga aattaatata tttagctgat tattaactca
    20461 tagcaacatt tttaatacat gacttcagag tagcgatttt atttttctac atgcaagcaa
    20521 ccatccaaag caatcagacg gtttaatatt agccaggttt aattgtgaag ttgaacttga
    20581 agaaaaatca cattaaaatt tttcaggata accctctaaa aaagggaagg aaggcaaaag
    20641 ccactttaca tgaaaattat taagtataaa aaacgcttaa actttggtag gcaagatata
    20701 cctgtggagt aggtgcccgt taaaccaaat gcaccaagac ttatcaaaga accgaaataa
    20761 aacacgagaa ctgaatgtgt tattcgagct agcctcacca tgtgtatgca ttttcccctg
    20821 ggactacaag gcttacctgt tcactttaaa tagtacagct aataccgaat taaaatattt
    20881 caacctccat gcaaagcgca ctccagtttg tcaacataac ataaattttt aaaaaagtgg
    20941 taaattgtaa accatagtaa tagctctatc agcctgaacc aagagcattt gcctaagcaa
    21001 aagtccgagg aaatgttgat tgaagtaaat ttgggttaca aagaatcttc aagagatggc
    21061 tcgtttgtga gttttaccag caaagccatt attttaaaac gacaaaatgc taatgattgc
    21121 aacaataagt aaactggtag gttggcttta ttttttcgga ctagttctct attcatatta
    21181 atactggcta ataaatataa agatgtaaag aatcatgatt aagcaggttg aaatccaaaa
    21241 cttcaaacct aggtgtttcc cggccaaact tcttatccaa ggcatccagc aagcatggcc
    21301 tgctaccatg gtaaatcagc atatatacca gcgaaataat aacccaataa aatttcaggt
    21361 ggaacattca tgcagggcag cttgcacaaa cgcgccggaa caaccaggcg atactagtaa
    21421 gttcggcgag gccaaaaaat gggaattcca tctgttcaac tccataccta aatggtatgg
    21481 ggcaaagtac ttgtaatcaa aaacctaaaa atttttcacg aatgaaaaga aaatctttca
    21541 aaacagctat gctcctggtc gccctaggaa gtagcttatg ggcacaaaat agttcaaatc
    21601 ctctatggat gcggtatcca gctatttcgc ccgatggcaa acacattgcc ttttcatata
    21661 tgggcaacct atttagggtt tcgagcgacg gcggaatggc agagcttctg acctccaatg
    21721 cagcgtatga tttcagcccg gtttggtcgc ccgatggtca aaccattgcc ttcgcatcaa
    21781 accggtatgg caattttgat gtattcacca tccctgccat gggaggagag gcaatgcggt
    21841 taacctttca ttcagctgat gaaattcctt ctgcattttc acccgatgga aaaagcatcc
    21901 tattctcagc gtgcatacag gacaacgcta acaacattca attcccttcg ggagccctat
    21961 cggagcttta ctcagttcca actcagggag gtcggatagc acaaattctc actactccag
    22021 ccgagcaggc cgtttacaac ccagagggga ctgaaattgc ctacaccgat ataaaaggtt
    22081 atgaggataa atggcgaaaa caccataaat cgtcagttac ccgtgatata tggatttatg
    22141 atattagcac cggaaagcat cgtaaggtaa ccacttttga gggcgaagac agaaacccgg
    22201 catttgctcc cgatggcaaa tcgcttttct acctctcaga aatgcagggc aactcaaacg
    22261 ttttctcggt tagcttatcc agtatagggc aaccagagtc tgtttcaagc ttcaccaacc
    22321 acccggtacg attcctttcg gtggcccgga atggaatgct ctgctaccag tacaacggag
    22381 agctatacac tcaaaagcca ggtagcagtc cgcaaaaggt caacattcaa atagctaccg
    22441 accaaaccga aacccgggta aagtatgaaa acttatccac cggcattacc gaaatgaacg
    22501 tttcgcccga tggtaaggag gtggctttta ttatcagagg tgatattttt gttaccgcta
    22561 ccgactaccc taccaccaaa aggataacca acacgcccga tcaggaacgt tcggtcagct
    22621 tcagcgccga tggtcggaag ctactatact cctcagaacg cgacggtagc tggaagatat
    22681 acgaatcgtc aatttcatca gccgaggaac caaacttctc actttccacc atcatcaaag
    22741 aacaggttat tgttgacata cccgaggaag cttttcagcc cctatactcg ccagatggga
    22801 aagaggttgc ttttcttaag gaaagaacga cgctttgcgt ggtaaacctc gaaacaaaaa
    22861 agattagaac cgtcctcgac ggaaagtata actattccta tagcgatggg gatcagtggt
    22921 tccaatgggc acccgacggg aattggctag ctgtaaactt catggaaacg ccttcgtggc
    22981 ccgattccga tattgccctt gtaaaggcag atggttcagg agaatttcac aacatcaccc
    23041 aaagcgggta cgacgaccga aaccccaagt ggaccatgga cggaaaggct atcatctggt
    23101 taaacgacaa aatggggatg agaagtcacg gctcatgggg ctcacaattc gacgtgtatt
    23161 ccgtttttct tacccgggag gcttacgatg agtttaaact gagcaagcag gagtacgaat
    23221 tgcttaaaga aaaacgaagt aaggataaaa aagacaatgg agcaggcgag gataaagaaa
    23281 gctcatccga taagaaaaag gaggtaaaag ctaaaaaaga caaggaagca caacgcaaac
    23341 ccctaatcat agactatgaa aacctcgatg accgcaaagt tcggctcaca ataaattcat
    23401 ccgacctctc cgatgcaatt ctcaccgatg atggggaaaa actctactac ctcagcaagt
    23461 ttgagaaggg atacgacctt tgggttaaca accttaagga taatgaaacc aagctgctgg
    23521 tgaaaattga tgggtttgcc ggcgatttgc aaattgataa ggataaaaaa ttcctgtttg
    23581 taaactccaa cggcacaccc atgaaaattg agattgccac caataagctt tcgccaataa
    23641 aatttaatgc ggagctggaa acaaactacc cccgtgagcg ggactacatg tttgagcaca
    23701 tgtggcggca ggtggaaaag aaattttacg accccaacct gcacggggtt gactggaaat
    23761 tctacaagag cgagtatgcc cggtttctcc cccacatcaa caacaacttt gattttgccg
    23821 agatgatgag cgagctgctt ggcgagctca acgcatccca taccggttgc aggtacaggc
    23881 ctaaaccaga gaatgcagat caaacagctg cactaggagt tttcttagat tataatttcg
    23941 atggaaaagg ggtgaaaatc atagagataa cggacaactc cccgctggtg aaagccaaca
    24001 gtaaagtaaa accgggttgc atagttgaaa agattgatgg cattgccatt gattgcaact
    24061 ccgattacta caaaatgctc aaccacaagg ccgataagct aaccctactg agcttttata
    24121 atccccaaaa cggagaacgg tgggaagaat cggtaaaacc tatcagcagg tcaatagaat
    24181 cggagctgat atacaagcgt tgggtcagga aaaacaatgc caccgttgaa aaaatctcga
    24241 acggccgaat aggttatgtg catgtgaggg gaatggactc gcagagcttt agggaagttt
    24301 actccgacat actgggaaaa catcgtaaca aggaagcaat cattgttgat acccgattta
    24361 acggtggcgg gtggcttcac gacgacctgg ccaccctcct ctcagggaaa cggtatgttg
    24421 attttgttcc ccgaggccag ctaattggct cggaacccat atcaaaatgg tataagccat
    24481 ctgtggtcat cataagtgag gggaactact ccgatgccca cggtttccct tacgtttata
    24541 aaactctagg cgttgggaag ctggttggta tgcctatagc cggaaccatg actgcagttt
    24601 ggtgggaatc gcaagttgac cccaccctgg tttttgggat tccacaaatg ggtgttaagg
    24661 atttaaacgg caagtatctg gaaaatcaac agctgaatcc cgatattaaa gttgccattt
    24721 caccggagga agcccagtcg ggcgtagata gccagctcat aaaagcggtt gaagagctgc
    24781 ttttagagct caaccaaaaa taggactcaa taatgtcatg gaaatttttg tagtaaatga
    24841 tattatttat acctttgtaa ttgtacaaac taactaaatc tatactacta tgaaaaagat
    24901 tgctttattt gtaattgccg ccggaatgat ttccatggtt gcatgcaagt ctgctccaaa
    24961 acaggaagag gctactgaac aacctgctac tgaacaaact cagcaagaaa cagctcagcc
    25021 cgattctgca gcacaagctg ccgaaactcc tgctcaacct gctcctgcac agtagttgtt
    25081 gctataagga caaataaaga caccccatat ttttaatgtg gggtgtttta ttttttaaaa
    25141 aagggtagtg ccagtaaaaa ctactcctta atgatgatat tcagctcatc aagctgctgt
    25201 tggctaatgg atgaaggcga gtcaatcatc acatcccggc ccgcattatt cttcggaaag
    25261 gcaatataat ccctgatgga atcggagcct ccaaaaaggg agcagaggcg atcgaaccca
    25321 aacgctattc ctccgtgggg cggagcgccg tacctgaagg catttaaaag aaacccaaat
    25381 tgtttttccg cttcatcatc ggaaaaaccc aacgtgttaa acatggtttg ctgcagctcc
    25441 ctgttggaaa ttctaatgga tccaccacct acctcaactc cgttgataac catatcatag
    25501 gcgttggccc gtatggccgc aggatttgat tggaataagg gtatatcatc gggatggggc
    25561 gaagtaaagg gatggtgcat ggcaaaaaac cgctgtaact cctcgtccca ttcgagtaaa
    25621 gggaagtcaa ctacccatag gggagcaaac tgatttttag ggcgtaaccc caagcggttt
    25681 cccatttcga gccttaactc gctaagtgca ggaagggttt tgttgcgctt gcctgccaaa
    25741 atcagcatca agtcgccagg tttggcttca aacactttgg caaccagcat caaatcgtcg
    25801 gcagagtaaa acttgtcaac cgacgatttc agtgtcccat catcgttgta gcgaatataa
    25861 accaaaccgc tggctccaac ctgtggccgt ttgacaaagt cggtcagctc atcgagctgt
    25921 tttcgggtgt agctcgcaca tccccgggcg cagattcctc ccacatactc cgcattatcc
    25981 acaaccgaaa agcccttgcc tttaagctgt gcaccaatat ccttgaagga catgccaaat
    26041 ctcagatcgg gcttgtcggt tccgtagagc gacatggcct cgtggtaggt cattcggcga
    26101 aagggagcgt cgaattctac ccccttaaca gtatggaaaa gatgcctggc cattccttca
    26161 aagacatcta gtatatcgtc tctttcgaca aacgacattt cacaatcaat ctgggtaaac
    26221 tccggctgcc tatcagctct caagtcctca tccctaaagc accttacaag ttggaaatac
    26281 ctgtcgaaac cagcaaccat gagaagctgc ttgaaggttt ggggggattg gggaagtgca
    26341 taaaactgcc cgtggtgcaa acgtgagggc acaacaaaat cgcgtgcccc ttcgggtgtt
    26401 gatttgatta gaaaaggggt ttccacctca agaaatccct tattgcaaag gtatctccga
    26461 acctcctggg ccatggtatg ccgcaacatc agggcgttct taacgggatt tcttcgtaag
    26521 tcgagatacc ggtacctcat ccgtagctcg tcgcccccat cggtatcatc ctcgatggtg
    26581 aacggtggta attccgactg gttcaaaatg gtaatattgc tggtgcgcag ctcaacctca
    26641 ccggtaggat tttttggatt tttacttgtt cgctcaacca cctctcccct aacctgaacc
    26701 acaaactccc ttcccagctg ttcaacaatt gaatgtaatt cgttactgct tttttcgtca
    26761 ataacaacct gggttactcc atacctgtcg cgaatatcaa taaaggtcat tcccccaagg
    26821 ttacggatcc gctgcaccca accacaaatc gtaacaacct tttggacatc gctaattctg
    26881 agttctccac acgtgtgtgt tcttaacata cggatatttt ttaacctgcg aatgtaaaaa
    26941 taaaaaatga cccatacgca tagaattgct atttttgaag ttgacaggta tccctaaaat
    27001 cctttgacaa tgaacttata ctcccacgag tattttatga acgaagcgct taaagaggcc
    27061 aaagtggcac ttgagctgaa agaggttccc attggggcgg ttgttgtgtg caacaacaga
    27121 attatttccc gggcccacaa cctgaccgaa acccttaacg atccaaccgc tcatgccgaa
    27181 atgcaggcca ttactgccgc tacaacctat ttaaatggca aatatttaga ccaatgctcc
    27241 ctttacgtta cccttgaacc ctgccccatg tgcgccggag ccatgttttg ggcacaactt
    27301 ggcaagctgg tgtatggtgc ttccgacccc aaaaggggct atagcctcca cggaaattca
    27361 attttacatc caaaaactct tgtagttggt agtattctta ccaacgagtg ctctggcatc
    27421 ctggtcgatt tttttagagg tagaaggtag ccacagcccc tgctgttgtt attcctacct
    27481 aatttcaccg gtaacaatgc ctctaccctt cgcaccatca ttcaacacaa ttccactgca
    27541 cttcccattt tgcatgaagg tatttaaaag gaaaccttct caatgcttat gcccgattga
    27601 taacccgaat aattttcatt aaaagttcct gagtataaac aagcactaag ttgctctaaa
    27661 tgttttacat ttgcatgatt tttccctata aatagcagcc caacttcacg ggtctatgta
    27721 ccaacctgct caaccaaaaa tagtattttc agatattgat ggaaccctgc tgaacggtag
    27781 caggcagctt tccgcaacaa ccattcaaac catcaggaaa attacagctg cctatgacgt
    27841 ttactttata ctagtctctg ccagaatgcc catggcaatg caggctatct atgatgagct
    27901 ctgcctaaaa acacccatta tttgctataa tggcgccctt ataatggaaa gcctagctgg
    27961 tggtttcgac aatcaaaggg aaatatgctc ccactccatt gacccccgta ttccgctatc
    28021 catatttaac cacacgctgg agcataacct gcacttcggg ttatacagca ataacagctg
    28081 gtttgtctca aaaaatgatg tgtggacaga acgtgaggaa aacaacactt gtgttaaagc
    28141 ggttatagcc aacaacatgc caggtataat atcacagctg gccagtgccg gtgagcccat
    28201 acataagctt atggtcatgg gaagccctgt tgaaatcgac ttgctcataa atagtatttc
    28261 cctggaaagc aggaacaaaa ttaccattta caggtcgaag gaaaccttcc tggaaatatc
    28321 accagcacaa tcgagtaagg ccaaggcctg ctccttactt ctcgattacc taaaccttaa
    28381 acccgagcag gcagtagcct ttggtgataa ttttaatgat atggaaatgc tcaggatagt
    28441 cggaacaggg gttgctatgg gaaatgcaag tgaggaggta aagcgacatg ccgacaaaat
    28501 tgcaccgcca aatacggagg atggggttgc ctttgtgctg gcagaattct tcggaatatg
    28561 acctcattgg aacttctatc accggcaaaa gaccttaaaa caggaatggc agccattacc
    28621 catggtgccg atgctgttta cattgcagca taccgctttg gcgctcgcga aaaagccggc
    28681 aactcaattg aagatattgg taagctaaca gaatttgccc accagtactt tgccagagtt
    28741 tatgtaacag taaacaccat aattttcgac aacgagctgg acgaagtcca aaacctaatt
    28801 aatgaactct accgggttgg ggtggatgcc attattgttc aggacttggc cattctttcc
    28861 atgaacattc ctcccattgc cctgcacgcc agcacccaga tgcataccaa tactctggct
    28921 aaactaaagt ttctggagca gtgtggtttc caacgggtag ttttaccacg tgagctaact
    28981 ctttccgaaa taggcagcta ttgcggctca acctccatgg agattgaggc ttttattcac
    29041 ggtgcgcttt gtgtttccta tagtggacaa tgctatatga gccagagaat taccggccga
    29101 agcgcaaatc gtggtgagtg cgctcaacca tgcaggtcgt catacgacct ggttgatgag
    29161 catggcaaaa ttctggtcag gaataaacac ctgctatcac ttaaggattt gaacctgaca
    29221 agccaaatcg agcagctggt tgatgccggg gttacctcct ttaaaattga gggccgcctg
    29281 aaggatatta cctacgttaa aaacatcacc gcccactaca acaaaatact taactccatt
    29341 atcagccgaa gaccagaatt taggagagct tcttcgggcg tgtgcgagtg cagcttcgat
    29401 gccgatcccg agaaaacatt caacaggggc tacactcagc atttcatcaa cggtcgaacg
    29461 gttaatcaaa cttcattcca cactcagaaa tcggtaggta aacccattgg gatagtagca
    29521 cgggtaaatg ctgattattt tgaaattaac agctacgaag ctgttcatcc cggggatggt
    29581 ttatgctact tcaccaccat cggccagctt agcggtatgc tggtaaacag ggtaatgggc
    29641 aaccgtattt atccacgtaa caaccccaca gggctaagcg agggtgatac catttaccga
    29701 aattcagaca tcgaatttga aaagcagctt aacggaaaaa gcgccaggcg atgggttgga
    29761 gtgcgcatag ctgtaagcga caacaacggc aaccttaagt tcagcgccac cgattccgat
    29821 ggtattgctg ttgaatatac cctcaacaac aactttgacg aggcaaaaga caaggcaagg
    29881 tcagtggaaa tgctcatccg tcaactctcc aaatcgggcg attcgatttt tagggttgat
    29941 ggggttgcgg tgcaggacta cactactcca aaatttattc ctatctccac ggtaaatgag
    30001 gccagacgaa tgctactaaa caaccttatc ttagaaagat taaagcagca cacccggccc
    30061 tttcgggata aatattttcc cgctgtttta taccccgagc aagccattac ctacaagggg
    30121 aacatttcaa atacagccag ccaggatttt ttactgaaac acggggtaac ctcaatagtt
    30181 gaagctgttg aaatctctgc tcccaaggga gtcttcgagt tgatggttac ccgatactgc
    30241 atcaaattcg agcttgggct ttgcccatcg aagcagaaag cttcacccac agggcagctc
    30301 tatctccaaa acagctcaac gctctacccc ctggttttcg attgtaacga gtgccagatg
    30361 aaggtgatga gtccaaaata gccatagaac ttattgtgga agaaatgaaa caccaatgaa
    30421 gggcggcttg tataaatacg cttgaacaac cgggcgttac ttacgggttt ggcgaagcca
    30481 aaatattggc gttccatacg ctttacctcg atacctataa ggaataacat aatgtatttg
    30541 taatcagaaa cctaaacatt ttatactgat taaaattctt aaaaaatctg cataaaactg
    30601 aaaaaacagg tattttcgat aaagtttctg attcgggaag tgctaaagca tatacccgcc
    30661 acagcttaaa aagattgcag atgattcaac ttttaactcc cgaaagctgg ccagagtacg
    30721 agcttattga cagtggtgat ggtgaaaagc tggagagaat tgggtcgcaa atggttgcca
    30781 ggcccgaacc acaagcgtta tggaggaagc aattatcaaa tgaagaatgg gtcaagcttg
    30841 ccgatgcgta ttttgttaaa agcaaaaaaa ccaaaccgac cgaaaatgag tggggtgaat
    30901 ggcgcctaaa gtcgggggca aaagagcaat gggtggtttc atataaccag agcggcctgg
    30961 catttcgcct acggttggga ttaacctcgt ttaaacacgt aggcattttc cctgagcagg
    31021 cggttagctg gaattatatc tacaacacta ttaagcagct cgatttggtt gaaaccaagg
    31081 tgctaaacct atttgcctat acgggggcat cgtcgctggc tgccagagca gcagaagcaa
    31141 aagtaaccca cgttgactcg gtaaagcagg ttgtttcatg ggccaatgag aatatgctac
    31201 tttcaggaat ggataacatc cgttggattg tggatgatgc cttgaaattt gtaagccgtg
    31261 aagtcaggcg gggtaacctc tacaacggaa ttatactcga tcctccagcc tacggcaggg
    31321 gccccgatgg agagaagtgg acagtagagg agaacctcta ccaacttctc gagctgtgca
    31381 agcaggtaat ggaacctaaa aacaacttcc ttgttctcaa cctatactca atgggctttt
    31441 cacccttaat agccgaaaac ctgatacggg acattttcgg cgttcccagc tatgaggtgg
    31501 gcgaactttt tgtttccgac agggcaggcc gcaagctgcc gcttggaatt tttgtcagat
    31561 ttcgctctgg tagtaatttt taacttgctg attggttaat tgttagccca ttttaaaggt
    31621 gttaaaaaaa atgatggtga atttagtaag gtgtttttat tacctttgtc aagattttgt
    31681 atatatttga ttatcaattg tacttccaac aagattaggt tttaaaattg gagagcttat
    31741 ggaagcaata aaaatacagg gaaccagcca atatcctact attgtgctcg acagtgaagc
    31801 aggaaggttt gaattctcag ggaactcttt gcccgaggat ccaaaagcgt tttacgagcc
    31861 aattcttagc tggctcgacg catacgccca gaaccccaat cccgaaacag ttgttcgctt
    31921 taagatggtt tactataata cccccagctc aaagcagatt ttccagattt ttaagaaatt
    31981 tgaaaaaatg gctgcttcgg ggcataaggt ttcggtggtt tgggcctaca acgaggatga
    32041 tattgacatc aaggatgccg gaaaagacct ctccgaccac ataaaactcc cattccggct
    32101 tgagtcctac aaggaataaa aaaaggcctt tcaggccttt ctttttttag ccttcaagtc
    32161 ttctaccaat cagtagtgta gctatggttg tatatcctgt aagttttaga ctcaccggtg
    32221 ttgtttgacc tgtagtagat gtagcccgaa attaacgaaa gagttttacc tgccgaggat
    32281 gtcccggcaa tcttaattgg ctgttcaaca ttcaacttga ggttgtggtt gccctttccc
    32341 tgaaaactct ggccaatgtt atcaataaca acaggaggaa tccctttcca ctcaatcacg
    32401 accttatcaa tggtaaaggg agaatcgtac ttcgaacgaa ccgtcatgtc cagcagagcg
    32461 ctaaccacat tagtgctatc ttttgatatg gcagtaaagt tgaagggatc gctcacaaat
    32521 attgaagcct tgttggcagc aacatcaaag tagaagggtt tttgaacgtc ggccactcca
    32581 aatctgtact ttaccagaat gttgttttcc ggatcctcgt agcagttatc gtagtcgtac
    32641 ttgatggtta cgttatcaac attgttaaca attgaggtaa cccttgcgca ttccgattta
    32701 acaacaggtt tttttgttgt tgaaatcgaa cgccctccga gtcggaggta gtacttaagc
    32761 tctcccaggc tgctctccag ctcaaccaac tcaatggagt taagcagcga gcggtacctg
    32821 gtgatcccta ggttgcactg gtcaaccctg gcatccatga agtactcctt tagacttgca
    32881 agttcggcca tgttcttttc aatttgctca accgattcaa cctggtcaac ctgggctagc
    32941 aaatcttcca gctgtcgggt caactcgtta tcgcgcattt tgaaatcgct taccagcttc
    33001 atcagctcaa actcaggaaa agggtactta atgtgatagg tataaattat gcttttgtcg
    33061 ggacgctgga gcttctccca gtagaagtct tcaacctttg atagcgaaat gccctgcagg
    33121 aaaggcacct tacccgactg ggccgttgtg gtggaggcaa acttttcgag gaatatattc
    33181 acattattgt tgtagttagc ctcttccttc tgaatggtgg aggtagattt aacattttgt
    33241 gcaaccgacg taacgatact ctccttaatg gaggttaaag cattctgctg cgcaacctgc
    33301 aaatcggggc cactgccggc aacaattatg taatccttct ctataccatt aacccacttg
    33361 ggtttctttc cgcttttatc caacacctta tcctgcgaaa aggtatatcc tgccaggcaa
    33421 accatcgcgg cggtcagcaa aagaattttt ctcatagcta attaaatttt cccatcgtaa
    33481 agaatgggag attataacaa ttcaagcact taccataagt aatcagcaac cccgatgggg
    33541 gttgctgaat tcttgggtta tcaaattaag ggcgttcgct ttccagcttg ttcatctcct
    33601 cgttgaagat ctgctcgaat ttcatcttgt cgtagtcgag ctgcagcttc tgatccttcg
    33661 aaatccggtt ggaaatgttg ttcagcacct catccttgga aacctggagc gcggtgaaaa
    33721 cctggaactt tccatccttg gtttgcatgg ttttattgca agctacctca acgttgttga
    33781 gctgctggtt tacaacctca cgcgacattt gctcgaactt ctgctcaaat tctgaattct
    33841 cgccaatttg gcgctcgtta acatacctgt cagataccga cttaatggtc gtgtttatac
    33901 tgcgagccag gttagtattg gcatcgagcc ttgccttact cttggcagtg cttaggtcct
    33961 ggctgatgcc attacctgtt ccacggaaaa acttggcatc ggaatgaaac tcgtcatcat
    34021 tgcagggaac tgaaacttcc ttcccgaatg aagaatctgc agcctttttg gaaccaccgc
    34081 agctagataa aacagctgca aaggtaaaag cagcagcaac cataatcata cttttcattt
    34141 tcatagcttt caatttaaag ttaaacttcg gattaagtta aaataatggg tagaaaaatt
    34201 ttttgctagt tgtgaattac tttttaacgt aaagccagct ccaatcccag ttgaagtagg
    34261 aggcagcgcc ccagtatgcc cacgactggg caacttcctt gcctgcctgt atcatcatct
    34321 tgtccttctc aatggaatcg tcgtactgct tcatcttgaa atcccaaaca ttagccccgt
    34381 cggcaagctg cttctgggtt atctcatcgg ccagcttcac tgcttcgctg tagcactcca
    34441 tatcgggggt aattttcccc aggtactcgg cagccttgtc aacgttcatg ttggcccacg
    34501 cagcttttgc tccagccatg tactccgtgc acttgtagtt ggcgtactgc ttgtatatct
    34561 ctacggataa atccatgctc ttgtcgaagc actccatgca aacatccgga acggagagca
    34621 ggatgtagag ggcctcctca tatttcttct gaccggcaag ggcctgggcg cctttcagca
    34681 ccacgtcgca ctgcgagttg tagtactcta ttatcttttc ctttcccttt tcaacaaaac
    34741 ccttgaactg ccctgctttt acgttgactc ccttcagcgc ttgaatgtag gccttatcct
    34801 ccgtctgccc aacaccctta acctgaatgc tggtctggct gaagatgttc tggcttgcag
    34861 cgtcaacaat gtagaaggtt acttcaaggt ttacagcctg cattggcggt gctgttgggg
    34921 ttatctcctt gcttaacacg tttaccatag gcacaatgca gaaccggggg tttgcagccg
    34981 ctgcgcccag cccattctgc acggctattt gctccatctt atttaccagc tgctggcgag
    35041 ccgattgggg aatgccctcg gcctgctcgg gaactatcgc tgcaagggca atgcgcccca
    35101 agtcgtcggt tttaccctga ctattttggg cacccaatcc aacggtaaat ccaaggatgg
    35161 tagcaacaag aatcaatatt cgtctagtca tcacctttta ttttctgatt aatagcattt
    35221 tacttttcac caaggattag ccaggcctct cctagccctc tcaggtataa ctttgaagga
    35281 atgcccattc cgttgaggta cttgcgcaag tcgcttacga acgaacgggt gtcaaccgca
    35341 cgctcccgac ccttatcgtc agtacgcatc atgggaatgc gcacctgctc aaacttcatg
    35401 aaattttcgg tagcatcgga aaggctgaag cggccctgaa cgcagttgtc ggcaaaccag
    35461 ttctcgatga tggttgaaag ctcctcggag ttgtcgccaa attccgattc gaggttttca
    35521 ccccagttgc tccatacctt aatctgaacc ttcacctcgc gaccatttgc aaacatatcc
    35581 tcaaaatgcc tcatcagacc tgcattaaag ttgtccatgt ggctgagaac agcttcctcc
    35641 aggagaagct cgggtgaggc agccgaagat gggttaccgg cacctgccac gcccgatacc
    35701 tgtttggcgg tgtaggcatc aaggccttta aggttgaaaa cgatgtactt ttggggtccc
    35761 tgccttttca cctcgaagtc gaggtccatg atgatgtcgg cttttgcaac acgctttaat
    35821 gcgtcaatag ggctttcggc catttcggag cctgaagatg agctggtaag catggatgat
    35881 tctgctcttt cttgttccaa attcttcatc tcctgctcca gatccttaag cggaaatccc
    35941 ctgtcggcca taatctgtcc cattttggta attaccaacc gtaaatcggg atcggtttgc
    36001 agggcagttt tatagtcggg aagggtttgc ttcattccct ggttatcaaa caccatcttg
    36061 tagccccggc ttatgcagta ctggtcgctg ggcactacca taataacggg tttctttgcc
    36121 tgaccaaaca ccgccagggt agaaaaaatc agaattgatg aaaaaagtag cttcttcatg
    36181 gtaggttaat agttagaagt taaaatccgg aatccaacct gcgggcaatg ccatcggcct
    36241 caagctgctt gcgtagggca tcgtacatca cctgcacgta tatggccacc ttgtagccct
    36301 ttttaatttt tatacgatcc tcgcccgatg gatcgccatc gttggtaacg gcaatgtact
    36361 ggaggtactt gccacctacc tcaaaaaact tttcgaagta gtcggcatgg gtaacctcgg
    36421 cgttgggttg agagcatatg gggggagttg gagcagatcc ggctccaccg ggtaaaccct
    36481 taaaaataca ggcagcaacg gcagcttcct tggcattcat aactgcagca cccacatcct
    36541 taccgtagcc ccacacctta aatacctttg tgccatcctg gccaacccca accggctcaa
    36601 tttcatagtt gtactggctg ttgtagagta ttttgcgttc ttttctagtt tgggcattgg
    36661 cggggaaaac tataacccat gccaagacca ctgctaacat tgtggttaat ttacctttca
    36721 tagcttaata tacttttagt ttagaacata attttaacac ctaatagggt cgaaactcca
    36781 ccgcggtcgg ggaagacctc atcccaggta acccccaaat cgccattatc atttgatgcg
    36841 ttgctgataa aggagtatgt gcctatgcca cccatcagct gtatgttatg tttcaaattt
    36901 agtgccaaat ttcctcccag cctcaggtaa agggtggtat aatttccagt attagcagcc
    36961 tcaatggcat cgctagtagc agactcctga ccaataccaa cgtatggacg caattccacg
    37021 ttgcgggtga gcatgaaccc cttggcaata cccgctccgt agtgggtaaa cgaaacatcc
    37081 tcggttgcta gggataaaat atcatactcc ttagtctgga atccaacttc accaaataca
    37141 aacagggacc ttattccaac aaaccttcca agcctgaagt cagccctacc gtaaacaccg
    37201 ccaacttcac ccatttcaaa tccggcaagc acctccaatc ccatgtcgtt ttgctggcga
    37261 accaggtagc cagtttggag cttacgccca gcagtttggt agaatgtgga ggtgggcatt
    37321 tcgccggtag ccacctggcg gttgtcaaca attttcgatg tagccctgat aactcccctg
    37381 aacctgggtt cggtggtatt ggttttctcg ttgtaaacat actcgtaggc aaagtagcgg
    37441 tggtcgcatt tcagcccctc cttcttccca attttaacgc ggatagggtt aacctgatga
    37501 atggtggttt tcaccataaa gtcctcgtgg ctcttttcca gattgtaaat ggtttcgtca
    37561 taacctttct gcaccagctc ctgcaggagc acatcgtcag atttttgctt aataaaccgg
    37621 ccaatctgtg tgtcggggtt aggctgagat gctacgatag gtacgctggt tttggttaca
    37681 aactcaagtg gaatttcgag ctgctcgaac ttcttaattt tttcggcctt aacggcaggg
    37741 ctatcctcag gatagatcca gcagtcgtat agggcattct gggtttcctc cgtatagtta
    37801 attttaaaaa gataacccac tgccgttgcc tgccacccat gcatgttgtt tatctttgcc
    37861 tcctgcatgg ttttcacatt ttggtaatca agcaccataa tgtagcttcg gccaactagc
    37921 cggctaccgt aatcctgaag cacagcgttt ccaagcttgg ttgattgagc cttaatgtag
    37981 gcagcatcgg tggcgttgaa catgcccctg tcaaaaatct tatccattga catggaccca
    38041 tcctcctgcc tgttgtacca taggctgata attgacttgg caaccttttg gtcggtcaga
    38101 tacttcttaa cagcctcctg cggaacaact tcagttgaag tgcgcgagaa tggagcatcg
    38161 aagctaaggg tctccatgtt gttattgtaa aacttgtcag aaaaaacaat ggatccaatt
    38221 ttcgactcaa cctcctgcac atggttttcc cccggaacct tcatgtaaag aaaggtgatg
    38281 gagctgcggt cgtaggttga gggaattttt tttggttgct cggcgttctg agcttttgct
    38341 cccaacacgg ccataattag catggcattt aagaaaagta gtttaatttt cataggtaag
    38401 tggttttggt tttagacttt aagtttaaat tatttcagaa aaaatttaga aactaaaatt
    38461 atgccgatta atacactaaa gcaaataaaa gtgattccac cggaatactt tagttgttca
    38521 aatataccaa ttaacctact acccattttc attcgtattg ccttgaatgg tgttcatcaa
    38581 aaaaaagggt ctcccctttt ttctacaatt ccgccgcttt aagtattagt ttgggaatat
    38641 cggtgttatc cagtaatccc ggaagttgcg atgatccccg acccattata aaaaccggca
    38701 caggcgagcc cgtgtgggag tggcttgtcc aaccaattcc tgcttttctg ttgagtatag
    38761 aaatggcatc acctgcaagt aatccagcgt ttttttgcgc attttttgat tcccctgcat
    38821 ttaggttgac taagcgctta taggcagctt caagggtgtt cctttcatca gcagtcaacg
    38881 caaatgcacc attttctgaa agcccgatcc tggcctccag caggccaagg taatcctcga
    38941 aggtagcttt aggattttta ctccataaat cgtaaagttc ggcttgaagc ctgtcggccg
    39001 atgccttttg aaggcttagc gctgtcaggt gcaaatcgta gccattggca ttatttccta
    39061 acgacattcc gcctgtttcg tgatcggcag taacaataat gagggtttcc gaaggatgct
    39121 tgctgtaaaa atccaacgcc acgttcactg ccctggatag agcaagcacc tcgtggattg
    39181 cggtagcagc atcgttgtcg tggcaagccc agtcaatttt accaccctct accatcataa
    39241 aaaagccctt agggttatca accacttcaa ttgccttgcg ggtaaggtca gccagtgaaa
    39301 tgtccgaggt tttacgatct atctcgtact gaagggtttg ctccggtgcg gcatttggcg
    39361 ttgagtaaac aattgatttt acgcccagca ttttaaggga atctacaccg gtaaaagaag
    39421 aggtaaagcc atagcccgcc ctagttccca actcataaat agaagcaaca gtgctccctt
    39481 ggtgtttgga gccatttggt tcaaggaaac caccggagcc ataaaagtta tacccgcttt
    39541 tgagcaaatc caaggcaatt tggtagtagc tgcttcggga gcctgcatgt gcataaaagg
    39601 cagcaggtgt ggcgtgatct atgctaacac tggtaagaat agcaacctta cgaccgttat
    39661 ccctgaacct ggttgcaatg ctataaaggg tgtcggtatg gtttgcattc agccctaacg
    39721 tcccgataga tgttttatac cccgttgata gggcagttcc ggctgctgcg gagcaggtta
    39781 cgtatcggtt ggcagcatag gttgttgcaa gggttgtgac agggaattgc atgaaggtaa
    39841 cccccagcgt atcgtgtagc acgctgtcgg catatacctg ggcagcatta acttgctgaa
    39901 tgcccattcc atcgccaata aacaaaaata cgtatttcgc ccctttctga ttctgggcag
    39961 aaataagaga cggaaatatg aacagaatta accaaatagg cagtaagtat cgtcttttca
    40021 tgatagttta gattagtatt ttaaacaaaa ttagtcggaa ttattaacaa aactattagc
    40081 tataagctat tatgatgtta aagttgatta acaagaattg ctattgtgag gcttgctagc
    40141 ctaatcggtt tgtatagtaa acagctccac cacagcggag attacaagtt gcaaaatatg
    40201 ccgattggat tgctacccgg aaggtgattg aaatattcga ggttattccc tggaaatttg
    40261 aacttaggaa ggataatggt ataggcagag caaccaggga attaatttac tgacaaaact
    40321 tgaagctaaa tcaaaattgg aaaggagaat tttagcgaaa taatctttat gaatccttaa
    40381 aaaaaaaatt gaagaacctt aaaatcgata tggaatatca gaagctagaa cacgaaaagt
    40441 ttctataaaa aagatgagga tatccaaaaa aaattgcgga gaaagtggga ttcgaaccca
    40501 cgttacgctt ttggcgtaaa cacgctctcc aggcgtgcgc cttagaccac tcggccatct
    40561 ctccggtaaa accaattcaa ctaactcgtt taaaatcggc cgcaagatac aaaaatattt
    40621 actaaagcta catagctagt aaagagtaat ctcgcctgca attggattgg caaattccaa
    40681 agccaaggca ctccctacaa atccctttcc taaaaggtac attaacttgg ctactgctgc
    40741 ctcaaaggta atatcgaagc cgcttgcaac gcctgcattt ttaaggatta atccattctc
    40801 gtatttgttc atattaacac ttcctgcctt acactgggta acattgagaa tagttaggcc
    40861 attcgcaaca gccttagata tcatttcaat aaaccattgc tggctaagcg catttcccga
    40921 tccgtaggtt tcgagaatta ccgccttaac ccctggtgcg cttaaagcag aggcaaccac
    40981 ttgaggagta ataccgggaa agagcttaat aacccctatg gagctgtcca tgctggtgtg
    41041 caccacaagc gaagaatttg cccggggata gtgtatgaaa ggatagttgt atttgatggt
    41101 aatgcccgcc tcggcgagca aggggtagtt atcggaacgg aaggcgttga agtgctcgga
    41161 gttgtgcttg gtggttcgat tgcccctgta caggttattc tcaaaaaaaa tggtaacttc
    41221 cggtacaaca ggcagcccgt cacgcttagc tgcagcaatt tcaatagcgc tgatgaggtt
    41281 ttcctttccg tctgtccgga gcattcctat tgggagttga gaaccggtaa gaacaaccgg
    41341 cttggcaagc ccttcaagca tgaagcttaa tgctgatgcg gtaaacgaca tggtatcggt
    41401 tccatgaaga ataacaaagc catcatactt ttcgtagttg ctgcttataa gttcggcaag
    41461 cttaacccaa aaatcgggtg taatatcgga agaatcgata ggtggatcaa aggagatggt
    41521 ttgtaggttg aagccaaact tctgcagctc aggcacctcc tcctcgatat tatcgaagtt
    41581 aaatggcgaa agggctcccg tatgagggtt ctccttcatt ccaatggtgc cgccggtgta
    41641 aatgatcagg atggaaagac tgctacgatt cttcattatt tataggcaaa ttaaacagtt
    41701 ttatggcgtt agccgatgtt agcgaggcaa cctcatgcac cggcatttcc aggatgccgg
    41761 caacccgctg cgcgatgaag gtgaggttga aactctcgtt tcgcttgccc ctagctggtg
    41821 cgggtgaaag gtatggggaa tcggtttcca gaactatctt ttcaatattt atatggggta
    41881 gcaccctatc aaccccgcca ttcttgaagg ttaccactcc gccgattccc accatgaagg
    41941 tttggtagtc cagtatatgc ctgtagtcct caagcgtacc ggtgaagctg tgaaataccc
    42001 ccgaaagcgt ggaatcggcc tcctcatcga gaatgctaaa aatttcctga aacgaattcc
    42061 tggcatggat agctacgggc aatcccagct tctttgccag ctggacctgg tgttgaaagg
    42121 caaccttttg ctcctcaaca aaggttttgt cccagtaaag gtcaatgcca atttcgccaa
    42181 ttccccaaaa ctttcgaata ctaagatatg agccaactac ctcaagttct tttgaaaagt
    42241 tacggttgac cgaggtggga tgtaaaccca tcaaaggaaa acactgggaa tacctatccg
    42301 cagtttctaa aagtcgcggt acagaagtag agtcaatatt gggtagcaca tggtacttca
    42361 ccccggcctg tgcagccctg tcgagcacaa cctccctatc gtagtcgaaa tcctcggcaa
    42421 aaatgtgtga atgcgaatct actaaaagca tgctgcaaga tgaagatagg tttaaaagca
    42481 aagttaagca aaattcgcat gttcaaaatt ggcaacctgc ctactccaaa atcaggatca
    42541 tgttaaattt taccttgatg cccgtaggct gtccccagca ggggttatgc tgtcgagctg
    42601 gtaaattata ggtgtaatgg cctgcctgaa cttttccaag ttttctggac gaatgggctc
    42661 caccggcggt gatttaacct tcagcggatc aacaggttta ccattcatcc agaaacgaag
    42721 atcgagatgt ggaccggtgg cgtagcctgt gcttcctaca tatcctatga tatcaccctg
    42781 ttttacccga acaccctcgg ctataccctt gccaaatctg gaaaggtgat tatagcctgt
    42841 ggtgtaaacg ctattgtgcc tgattttgat gtagttaccg gccgattcgt tgtaaccctt
    42901 gtctattaca accccgtcgc cgatagccat aacaggtgtt ccggcaggtg cggcatagtc
    42961 aaccccacta tgtggacggt aaattctcag aacagggtga aagcgccgac cgctgaagcg
    43021 gctcgatatt ctggaaaagc gcaagggtgc tttcaggaat gatttgcgaa gagaatttcc
    43081 cttttcgtcc cagtagctgg caacggaatc ctgttcatac ctgaaggcat aaaacttttc
    43141 accccgatgc tcgaaccatg cagcatgaat ggttcccagg ccaaccggca ggctgtcaac
    43201 ataaagctca tcgtaaatca ccttaaactt atcctcgggg tagagtccaa agaaatcaac
    43261 agtccatgca aaaacatccg aaagctccag tgccagcatg gggttgatgt ttttctgctt
    43321 catggtctcc cacaacgagg aggtaatcgc agactcgtca accctcctaa cggtggtaac
    43381 ttcctttttg aaagggtata tcgccagcgt gtcggaaatg tcaaaaataa caaaatcaac
    43441 gggtgagtct tcataaacca ggtatctaac cgcagcaagc gtatcgcggg ttctgaaaac
    43501 ttcgtaatga ttgcctgcct taaacttatg gggatcaaaa ctatcggcgc ttaccaaagt
    43561 caagctgttt acaacttcgg gatctacatt caaatcgagc aaaagattcc caatggtttg
    43621 cccataccgc aacgaacctt tttcaatgta gaatgaatca acaggaattc catattttaa
    43681 gatgggcttg gccaaaagcg tatctttaga atattcctca gaatttttac tcgtccttac
    43741 gggtgatatc cccacaaaat aatcaacaaa aaaaatggct agtaatgcca gaattactga
    43801 aaaaaatgct cttttcaaca taatatacaa aattctaaac aaccagtagg cgaaaattct
    43861 gcaacccaac tcctttgaaa gaactgctac aggagaaatg atgggcgtgt taaaacagcc
    43921 tcaaagctac caaaaagaaa ggttaagaag gggaaataat ttttaaattt cgtgtagctt
    43981 tgccaaaaat tggtggtatg agcaatcaag aaaggaaaac agagctttac gaattaggcg
    44041 aattcaggct tattgacagg ctaacggggt catttggcaa gcttcacgaa accaccatca
    44101 tgggagtggg agacgatgcg gctgtggttg acgttggttc taaatacatg gttataacca
    44161 ccgacctact gctggaaggg atacacttcg acctaaccta ctttccgctt aagcatttag
    44221 gctacaaggc tgttgtggtg aacctaagcg atgtgtacgc catgaatgcc acacctaccc
    44281 aaattaccgt ttcaattggc gtatcgaaaa gatttggcgt ggaggatttg gaagagctct
    44341 atgcaggggt gaagatggct tgcgaaaggt atggggttga catggtggga ggcgatacct
    44401 catcgtccat gacagggctt accattagca ttaccgccct tggcgaagtc tccaaaaccg
    44461 acataaccta ccgcagaggc gcaaaggcta acgacttgat ttgtgtaacg ggtaatctag
    44521 gggcagccta catgggctta cagctgcttg aacgcgaaaa gcgtgtgttt gaatcaagcc
    44581 ccggggcgaa acccaagctg gagggcaaca gctacatcct tgagcgaatg ctcaaacccg
    44641 aagttcgtaa ggataccctc cagaaactta gggacaataa gattaagcca accgccatga
    44701 ttgacatttc cgatgggcta tcgtcggatt tactgcacat ctgcaagaac tcaggagtgg
    44761 gctgcaaaat ttacgccgat aaaattccta ttgatagcca aacagcacaa atggctgaag
    44821 aatttggcat tgagccgctg gttgctgcgc tgaacggagg cgaggactac gagctactct
    44881 tcaccattcc gctggaggag ttccaaaacg tttctaaaat agatggaatc gcaaccattg
    44941 gtcatatcac cgctgaatca tcgggggcaa acctgatttc acccgcaggc gatgaaataa
    45001 ggctgactgc tcagggttgg aacgcactgg ctaaggagta gccttcaaaa taaaaaaccc
    45061 cggaaatccg gggttactca tctaacaggg ttgtctaaaa ccccaacagg tggcgcagga
    45121 aattcaccag cttcggcaat tactcgctta ccacactctt atactgctcg gcggttagca
    45181 ggttgttcac ctcagcaggg tttgtcattt tgattttgac aatccagccc tccccgtaag
    45241 gatccttgtt gacaacgtca ggttggtctg taattttagg gttcacctcc agcacctcac
    45301 cactaacagg cataaacata tccgatacgg ttttaacagc ctcaacagtc ccaaaaacct
    45361 cctcatgccc gagggtttcg ccaacggttt caacctcaat gaatacgata tcgccaagtt
    45421 gcgactgggc gaagtcggtt atcccaacag ttgcctcgcc gttaccttcc acttttagcc
    45481 attcgtggtc cttggtgtac ttaagatttg atggaatgtt cattgctata attttttagt
    45541 agttaatatt ttagttggta aaatattttc aaaagtaagc atttttgcaa ttgaaatcga
    45601 aagatactga aaaataaagt taatgcggag cagtattact ggttcacacc gggtaattgt
    45661 tggccttggt gtttattgcc ttagcacatc agccaaaagg ggtggcaggg agcaggagcc
    45721 atctacttaa ccagctcaaa cctaacgcta aacccgaagc tggtattggt tgtgcggtag
    45781 gttctggaaa cttttggggc gttgagggtt cggtcgaaaa acagccttac cgtaacctgc
    45841 tcgttgatgc ggtagtcggc ggaggttttg atggtattaa cgagttgtcc ggcaataatt
    45901 tcggagtaat cctccaccag cttgcgcaaa atggttgtgt tttcgcgtat ggagagatca
    45961 acagtaatcc gcaggtcgct cttaacggcg ctcttaccgc catcctccga ctgaaacaca
    46021 aggggcaaat tctcgaacct gtagcctgcc ccgataatat actcgtttcc gtaaatttcg
    46081 gtaagctggt tgttggcaaa actcattgcc agcgaacggc tggttttaaa ctcaagtcgg
    46141 gtggtgaggt tgttctgcca gcccatatcc aacccaagca atggagaaaa ttgctcgttt
    46201 atggaaacgt tggtaatttc acgttttggc acaaaatcgc catttatgtt gcgcacgtag
    46261 ctaaaaccat cgtcctcggg cagatagttc aggttggttg taaaggcgcc aatagtatag
    46321 gccgatcggt agccatgaga tagtgtaaag gttttaaaaa gattcttaaa tggtcgaatt
    46381 ttagcaagtc catcgtaggt aatctgccag ttgggcattg gaataaccgg aaaatctcta
    46441 agggtaacct tctgcggcga ggtattggta taggcagcca ggaatgaagc cctgagcaca
    46501 tcctgcgata gaggaccgta acccaccggg aatcccaaag agtctggtgg agccatcggg
    46561 tcgtaaccct gaccgggatt agccactcgt tgatcgcgaa gcctctcggc aattaccagc
    46621 cggttgctct tcatcctctc aaaagccctg gattttgcat cgcggttctt tccctcaaag
    46681 gtagttccaa gcgttagtac gctgatgctg aaattaccgg ttgtcagcgg gctaagcgag
    46741 ctatacgacc ctagtgcatt tgcccggtag gtttcgttat ggttgcgcga gtaggttcgg
    46801 ttggccgtta gctcaattcg aaaaccgggc acaggctcaa tgctgctgcg gaagttcaaa
    46861 ttttcgttgt gggtaaacac caccggcgta ttaaaggtgg tatcgtgtgt gagccagccg
    46921 ttggtagctg ctttccatgc aatttccgga tcctgccaac cggcaatgaa ctcccatccg
    46981 ggagccgtaa agccgttgta gctgtcaatt cctaagtacc gtgtaccggg tttgtaacca
    47041 ggcagaagcg ttccgttggt ttcggaatag gaaagcgaaa ggcttttaac gctcatcatc
    47101 aagcgaagtg tgccctccca cgcaaacttc agcggattgg gcctatcggg caccttgcct
    47161 tcaaccacca ccctgcagtt gctgtaatcg gcatctgccc tgatggcaac acgtgtatca
    47221 cttttgacct ccgatttaac ctttacctcg ttgcccttct cgtcgtacac ccttacagtt
    47281 accttatcgg ttttgagctt atggttgata ttttttgcgt acttcgctct aatgttcacc
    47341 ccgccttcct cgtacttaac ggttttggtt ttaggtgctg cccctcgccc cctactatca
    47401 aactcctggt taattcgctt aaggtagcca accttgttga aaagagtatt aatgttgatt
    47461 tgaccgttaa gctggatggt gttggagttt tgaacggtat tacctgggtc gaatcgcgat
    47521 gtatcggcca taatggggcc tgcctgccac cggtaaattc cagtgtagcg tgctgaggca
    47581 ttagtccagt caagaaatgg gatcttactt agtggaagcg agtaggaaag gttaaactgg
    47641 tggttataca tggtattacg gccaaaattc ttaatgttct gccacaccga gtcgcgccag
    47701 tgctcgtatc ctttagggtc gcggtttcga tcaaccatgc cctcgggttc atcaatacgg
    47761 gctacgttgc tggcagtaaa atcaaatttc aacgattggg taatgtccca gttcagagca
    47821 tagttgcgat tccacagaaa gtccttggag taggttggaa gcaacacggc gctggggttg
    47881 cttacgttgc gaaactgctg ttcaaagtaa cttcgatcca aatcggttcg gaaggagagc
    47941 tgagctgggg taaggttaaa gttgaagtcc ttaatcaacc tgaaatattt tgagttcaac
    48001 cacttcacgt ttttaaaggg cacaatgtac tttggctgct tgttgaagtt gtaggttagg
    48061 gcgcctctaa tatcccgttg aatcttatga tcggtgcgga tattccttga gaacagctcg
    48121 ctgtaggagt agctcacgga gaagttggaa atgttccaaa aatgaggttt ttcagtagtc
    48181 ttgtcaattt taacatttgt aaagttgatg cttcgatgtc gggagtaatc ctgcacaatc
    48241 cgctttacgg aatctctttc cgaagggttg taggaattca gggagctgct aagggttaca
    48301 tcggggtcaa gggggttata ctggggattg gatatggact cgctgaaccc caggaacatg
    48361 ggtattctta cgtttaactt ttcggggaaa aacttaccca gctccagggt ggagttgata
    48421 tcgtactggt aaatagtttc cttgctgcgc tcgttaacct ttttctcgat agagccaaat
    48481 ccgggagtaa cggttgaacc ggccacattt atcatcccta aatcggccag cttggccgaa
    48541 actcgggcat tagcggccca tcccccctga tcgttaaagt tggtaagtcg aaactcgttg
    48601 aaccaggcca ccactgattt aggcattccg tcatcggttg gaccctttcc gctagccggg
    48661 tttctaatac ctatcataat caccttaacg ttgctgaggt tgggatttcc caccaccttc
    48721 atctgggcat caccatcatc aaccgtgtag agggttcgtt gggttaccat tgagcctggg
    48781 gttcgcatgg catcgttacg ggccacctta acctccacca gcttttccag atcaatattg
    48841 attagattct cacgaggcca aaccatttcc ctatccgact cccggctgtt aaagtagtag
    48901 ccgtagggtg tcagggacaa gggaatttcg tactcgtagt agttgtcggt ataatcggtt
    48961 cctattcgga taaatgccga aacatcgcca tttgacaacg gataaccctc aaccatctcg
    49021 gcgtgcacgt ccattttaag ttttttgtac tgccggatat ctatattgac atttttgaaa
    49081 gccgctctgg catcgccatc ggccaggtca atcaccctgt actccatggc ctgctcgttg
    49141 agctcggtga gctgaggctg gctggggtca ataaccctgt caacgcccgg gggcagcacg
    49201 tagttaaccg gcgtgcgttt gtcgttctcc tcaatgttga ccgaagagat ggcaaacccc
    49261 gtggtgggta aatcgcccga aggaataccc tcctgcccct gaataagcga gtagttatac
    49321 ttacgccact cgcttttcac gagttcaagg gtggcaaatc tcaggatcat ggtgtcctga
    49381 aagccccgca ggaacattct catgaagcgg atggacttaa aatcgtcaat tgggccgacc
    49441 acccgctcat actccgtaat gggaatttta aactggtacc acttcaccgg aacgtttcta
    49501 agcttatcct tctctccgga tatcacgttg gtaatgtagt ttctgcccac ctccatgtcg
    49561 gcaggtcgaa gggatatcct gtactggtag tatgcctcgc tttcgctcat ggtgatgtcg
    49621 ccctcgctaa ggttttcaac atcgggcgtg gagtaggcct gctgggatgt ggttccggtt
    49681 gcaacaggtg agttgccctc ggtgttgttg aaatacttat acctgtcgag gatggtagct
    49741 ttgctctggt cgtactggtt gttcaggaaa tggatgaaat tatcgttgga cgggtcggca
    49801 tcggattttg ccataacctc ctcgttggat accctgaagc gcagctgggt aaggtagttc
    49861 tggaaaaatg attgttcatc gggaatgccg tcgccgtcga ggtcttcgcc tgaaaggcca
    49921 tcaagcccta tatcctggta cttacggttg gcggggtcat tgtcgaatcc tacaggaagg
    49981 tactgcttgc ggggcactct accccaaaca gtagtgtcaa ccttctccat ggcctgggtg
    50041 gtgggcatgc cattctcaaa cgacttgcga ccgtcgcgaa gtatatcctc cgaaacattg
    50101 ccaaggttga tgtaaaaatc gcctccgctc atggtggagt cgtaaacaaa gggatccatc
    50161 agccaaaact cgatgtactc tatgttggct gtttcaaagt cggtagttcc aatgctccgc
    50221 ataacaccac cccagcggtt ctgtgggtta agcagtcgtc catcagggtc aacattaata
    50281 tcatcgtagt tgtatggacc tcgctcatca gggtaaaagg acaggttcag caccgatatg
    50341 ttggtgggct gcccctgcgg tgtgttccgg ttggggaaca gctcacgttc gaaaatttcc
    50401 cgaacaaaat gcgatccctg cagctccttg ttgttgcgga tgtaggatgg ggtaagggat
    50461 gtattccgca aaaaaagcgg gtcgatgaag taccatgcca gcatcgctct attcatgccg
    50521 ttgcgcaggt cgttgctata agaggcctca gggaataagg caggttggcc ctggggtgtg
    50581 gaggcaagct tccactcggt ccagtagtgc tggtcgagct tagtgctgct cccctcaaaa
    50641 tcgtcaaggt aaactgttcc ggctttgcct atagcccttg agtgcccggg aataagatgc
    50701 gcaaactcgg cgtcgaaggt tacagttgac ttctccttgg tctgaataaa tggtaaaaaa
    50761 tcaaccatgc gggtaagcag ccgagagtcg gtttggtagg aggtattcaa cccccagatg
    50821 gtatttgaga tagcctcgtt tccaaaactc accttttggg taagcgggcg ctcggtaagg
    50881 ttcataatgg tggcgcccac gttgaagttg tcggagaacc tatagtcaaa gtggctgcca
    50941 attagtgttt tagtttgaag gctgaacaag gcgttgcttt ccaccgaaat tctaatggga
    51001 gtacccgatt caagcagcgc ctggtctaaa atgcgcaccg agccaagatt gtagtcaaca
    51061 atatactgaa catcctcctg aagcttagca cccccggccg taaccaccac tgaaccctta
    51121 ggaatgtttg gagcgttaag gtatatctcc gatccacctg ccgactggta ggagccgcta
    51181 agaataaact tgtttttttc ggccatctgc ctcgccctgg taagggtgga gtcgtatagt
    51241 tcctgaaaaa catacttatc ggcaatcgcc ggattggtaa atttagcctt caaatcgcgg
    51301 ccaaatggtt caagtaccgg aaaaattatc tttccccctg ccgggttaat ggttaccccc
    51361 tcaataaaat cgaacacacc atcgggtata gcatcaagct ggttgttgag gttatcgagg
    51421 tttaacacgc gaataagcgg gatgttggca atagcatcct cgccaatgta gcttaaaggt
    51481 gttcctgttt tatcgttctc gtagagtaca tcgagcctaa actcattctt gctaacctgg
    51541 aaagcgttca tggagtatat attcttcatc atcagcttcc atgttggcag ggaagggctt
    51601 aggttggtac ccttaagcag tttcacaaca agtgcttggg gtgcagcaat cccatcggtt
    51661 gaaagctcac caaccctgta ggtttttcca tcctgggtgt actcaaaggc aacagccagc
    51721 atttcgtcgt tatatagcgg agagttcagc gaaatgtagc ctaaaacagg gttaaaggta
    51781 aactcgttgg aagaaagccg gcgtgcattt tcaatctttt cgtaatcacg acccccgcta
    51841 aacccacttc cctcaagggg caaaagggtg gagttcacct cggctatatc gcgaatgccg
    51901 gagtatgttg tggtcatcaa ctcgtagagg gtgttccggc tgttatcggc cgggccggga
    51961 acggtttggt gaaaaataga gctggcaaaa atattcggat cggcctcacc caggtcggca
    52021 aatgccacca gatttcggga gttctcaaag ttggcctgct tgttggttac ccaaacttca
    52081 attttggtga tgttgatgcc cgagttaatc accggtaggt tggccagcgc cctgttatag
    52141 ttctcacgaa agtagtgcga caggaaaaaa tgccggttgg cgtcgtactc atcggcccga
    52201 atctggaagt cacgcacctg agcgccaccc ttaacctcaa ctacctgggt ttgaccattc
    52261 tgcttcgaca ccacactggt aacgtaaagc ttcccgaact tcatctcggt tttaaagcca
    52321 aaaaggcttt ggcttccggt aattagggtg cccggcaggg gaagtgatac gtttccagcc
    52381 tcaatccgct ggataatttc gtcctcaaaa ccgttgtact ccaccttcac gttattctcg
    52441 aaatcgaaag ttgcctcggt gttgtagttc acctccatct tcaatttctc gccaattttt
    52501 ccggccacgt tcatctggat cttcgactga aaatcgaagg tggtattttt tcgctggtca
    52561 accggtagtg cagggttttc ggttttagag gaggatatgc caaaaaccag ctcagcatta
    52621 ccctggggga taatctcaat aacattgctc ccaaatatcc tatcaaacga ctcgccaccc
    52681 acctgaatgc gtggcaaaat gccatcgccc tttccaactg tttcgccggt ttgcttttgc
    52741 cgccagtact cccgcatgga ctgctccacc cggtagttct ggtactcctc gtcgctcatc
    52801 acacgaggca aacctacgtt atacccgcca actcgctggt aaacagtata tttctttgtg
    52861 gtgggatcgt actccaccac ctcggtaaca ttcgatggct ggtttagaaa caggggtgaa
    52921 cgcaccgttg ccggtgttaa aaaattttca gattttattg ggtagggtag cctggtggta
    52981 tcctgcaggt taaccgtttg ctcatacact tgcccaccga tggatacacg cggggtgaac
    53041 agcagaaagc atgaaaatac aaacaacagc aaggaaccta ttttaacgcg tgccaaagtc
    53101 aatgcctctc cagtgataaa gttctacaag cgctttaatg ccgatttaat cagcccctcg
    53161 acagtcaact ccccgccttc cctgataatt tgatcgagca ccttttcggt ggcagacttg
    53221 gaaaaaccaa gcataaccaa tgcagataac gcttcatccc tcgaagtatt gtgtgctgca
    53281 ggcataattt gtttggaagc aaagtcatcg cccaccactt tatcctttag gtccaccaca
    53341 atgcgctggg ctgttttcag gccaatgccc ttaacctgct ttatcaggtt aatattttcg
    53401 gtaagaatgg ccgcctgaag ctcatcggcg ttaagcgacg aaagaattaa acgagctgtg
    53461 ctcgctccta cacctgaaac gctgataagc agcctgaaaa tttcccgctc ctttgcttcg
    53521 aaaaagccgt aaagcaaatg ggcatcctcc cttatcacct gatgaaggta gagctttacc
    53581 tcattcatac cgttgatggc cgagtatgtc tgcagcgaaa tgtttacatg gtatcctaac
    53641 cctccaactt cgaccacagc gtgggtgggc gtaagttcgg ccagcttgcc tacaatatag
    53701 tcgtacattt cctttacgtt ggtttgaatt aagtatgggc tattaggctg atttgtttgg
    53761 cgttatgagc cactactttc aggtagagct gatataagga tttggtggca agcacacctc
    53821 aacccattgc taaactaacc attttccgag ctagaaacgc tcaaagggtt ggtagtgagc
    53881 tggtcatgca gttttcggtt gttgaatatg tctatggcca ggtatagggc atttctaaac
    53941 gagttggggt tagccaagtt ttgcccggcc aaatcgtagg ctgttccatg atcgggtgaa
    54001 gttcggataa ccggtaggcc ggccgtgtag tttaccccgc tgtcgaagca aattgccttg
    54061 aatggggtaa gtccctggtc gtggtacatg gccagaatag cgtcaaaccg agtaaaacca
    54121 tcggaaccaa acaggccatc ggctgggtat gggcctaacg ctgcaattcc ctcatcagca
    54181 gcctgcttta tggcaggtat aattgccgat tcctcctcac ctcccagcaa tccggcatca
    54241 cctgcatggg gattaagccc caacactgct atacgtggcc tgcggatggc aaaatccctc
    54301 accagcgttt ggtttagcaa acgaattttt cccagtaccg tatcacgggt aattagcgac
    54361 gagactttcg aaagaggcac gtggccggtt accacgccta ccctcattaa atcgccaacc
    54421 ataagcataa ggaattcctt ggcattaaac gagcttgcca gatactcggt gtgtcccggg
    54481 aagctgaatt ttgcactttg aatattggcc ttgttaatgg gtgcggtaat cagca
//
LOCUS       DAOSHM010000012        54511 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_450_length_54511_cov_21.483197, whole genome shotgun sequence.
ACCESSION   DAOSHM010000012 DAOSHM010000000
VERSION     DAOSHM010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 54511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 54511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54511
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_450_length_54511_cov_21.483197"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            53..1117
                     /locus_tag="PK839_03310"
     CDS             53..1117
                     /locus_tag="PK839_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG71797.1"
                     /translation="MFGRILKTIGKVGSFLIIFLVFFLLYSSITYFDPESRVVLWQSD
                     KPDTIPFDSTLAALTWNIGYAGLGSNMDFFMDGGGKMRDTRERTTENLTAIEKTLGGE
                     KINDFILLQEVDINSHRSYGINELEEISRTLEYPYSIGINYRVNFVPVPLKNPMGKVQ
                     SGIVTFSKFNPKETIRYAYPGAFPWPNRLFNLRRCMVVNRYPTSNGKDFVLVNTHNSA
                     FDNGALKQQEMDFLQEILLSEYYKGNYVVAGGDWNQLPPGAPYAELVVGSSNSSFMLT
                     GIDSSFMPKDWSWVFDPSCPTNRYLNEPYTPGRTNTCILDFFLVSPNVSVQRCQTANM
                     AFKNSDHNPVTLEFRLIGNP"
     gene            1486..2325
                     /locus_tag="PK839_03315"
     CDS             1486..2325
                     /locus_tag="PK839_03315"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HOG71798.1"
                     /translation="MQVTDQDLQYFITTLKNVSKYDFSEYSDKSLKRRLQKILEDYNL
                     DIFSLIANLKNDRAFIEKVVKEVTVNTTELFRDPPVWHLLRSRILPRFRNNKTINIWH
                     AGCSTGQEVYSMMILLNEMGMLEQAKIFATDINIDVLDAAKQGVYKYRFNLGYLDNFD
                     KVIKQNPLNFEEYNEVPYEKYFEIDKVKDTITMNKFLSEKPIFRKHDLVTDGNIFFAK
                     FDLILCRNVIIYFNYSLQNKVFELFHSNLYPKGVLLLGMHETILGPWATKFEKLGQSY
                     VKK"
     gene            2501..4858
                     /locus_tag="PK839_03320"
     CDS             2501..4858
                     /locus_tag="PK839_03320"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOG71799.1"
                     /translation="MKISQRIQLIIVPLMILLFSASGVTLNYLSKNRVRESIRIQEGV
                     YIDKLIQIVDMVEAKTGSGLSSDDIFELRTYFLSPAYYSTDYPAIISTNGVYLLHTTL
                     QGQRLPSAATAQIRKSKSTDGYIYYDDYSGNKPEKRLLCYKFYKPYNAYIAISVSMNE
                     ALAPMKSNRMAIIVFIILASLLTAFAIPLLLKPIIRIINLVNIKLRQLAKGDIPNPIK
                     ITSKDELGEMVTSLNQLMEGLKSTTNFAHQIGQNQLEHTFQPLSSKDELGNALLDIQS
                     NLKHALEEEQRRKEEDYIRGWVNEGLAKFGDILRQNNNNLTLLADSIIQNLVNYLEAN
                     QGGIFIYNDSENDKHLEMLSAFAYNRKKFISKTIPLGEGLVGACALEKKTIYLKEIPA
                     DYIAITSGLGEATPTALLIVPLKLEDEIFGVVEVASFREFKPYEIEFVEKIGESIAST
                     LFAVKNSIRTNMLLEQSQQQREEMAAQEEEMRQNMEEMQATQEEMLRKNIELELITNT
                     INQSLLSLTLSEEGYIADSNQNFVETLGYSKTELEGKMLADIVNPEQRTDFQISWKKV
                     RAGQTITETIHVLNRNGNSHYLMVTVSPELDPAGAITKVLLIGQDITEMKQLEIKAQR
                     QAEEIEQNLKQLEKEQELSAQRQKDMSTLLEALDQNCLVSIIEPDGLITYINNKNVEV
                     LGDKKEDIEGKFLHDIDYSAKHRPAEYKKFWDNILNGKKQQREFSLTVKGEVKWVLEN
                     FTPVMDESGKLYKIINIGFDITENKNKEAELTKIIEQMKNKPKNL"
     gene            5005..5838
                     /locus_tag="PK839_03325"
     CDS             5005..5838
                     /locus_tag="PK839_03325"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HOG71800.1"
                     /translation="MMQYEIGIVETRNVIKTLLDDYGYDFRDYALTSFKRRLEQIISN
                     NGLKDADGLIGKLKGNPTFFDQFLNEVNIDTTEMFRDPSFWRTLRDDILPEIIRNSTT
                     KPRIWIASFDSGEELYSLTIILKEMGALDKVQIYSNVFTNATIQKITSGQLDPKDIEI
                     NEANYVRLNGKSQYSSYYKVEGNKYFFDSTLVKDVNFIKQTPIIDKDPGAVKLVLFRN
                     QALYYNQLLQERVLQKIAESLVAGGFLALGIKETLENTNANNKFTVFNETERIYKRKN
                     V"
     gene            5844..6404
                     /locus_tag="PK839_03330"
     CDS             5844..6404
                     /locus_tag="PK839_03330"
                     /inference="COORDINATES: protein motif:HMM:NF013502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HOG71801.1"
                     /translation="MYKAVIIGGSAGSFQVITRILHSLPASFPLPVLLCLHRLKHVRS
                     GFVEALSLKSGIPIIEPDDKEQIKPGKAYLAPANYHMFIELGNKIALSTEDPVNHSRP
                     SIDLSFLTAAQVYRDKLIGVILSGANRDGAYGLKKIKDLGGLAIVQDPNECQVRTMTE
                     ASLKMTPVDHVYNTSEIIRFLQTIKT"
     gene            6409..7224
                     /locus_tag="PK839_03335"
     CDS             6409..7224
                     /locus_tag="PK839_03335"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOG71802.1"
                     /translation="MKAFSSKSKLLPLLLLVLLIAGVVITFGLIHNTLQNTGGSLPAI
                     GYVLLFLPAIISIGLYAILNNLQVKSAKLEAEIAELKAAFALLQQVKNQEQANATNQE
                     ETVDYDKLLSRLIPTLPTDNLEKFGEALLSNIARNYEIVQAVLYTRDGDTNLFTFKAG
                     YAYFSESEPVTYSEGETLSGQVAKNRQILNLDQVPENYITILSGLGKGSPKHLLIAPI
                     VSPDNNSIGVFELAAFKPFDNMQVELFDRLGRKLGELLWSSRPETYLKHSAWT"
     gene            7267..9879
                     /locus_tag="PK839_03340"
     CDS             7267..9879
                     /locus_tag="PK839_03340"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOG71803.1"
                     /translation="MTSFRLTSVRETKNLTRNKIALAFLVGLLFSFAAWIADFSKSGL
                     TFSLQNIFRQHAANPTLWITDLIPFAFAYITWLQSTRFMRKTLRLEKELKQRDEDIKK
                     NADFAKKIGEGNYNAPFEVASEDDILGKSLLIMRDNLLTNQKKEWERSWIAKGKEEIS
                     YILRLHHNIDELSYDVLVKLIKYINIIQGALYIYDEEKQLLINMTTYAYNRRKYINQE
                     FRIGQGLIGECAYEKDFIYRTEIPDDYITITSGILGDKKPKSLLLIPLITDEKLEGVL
                     EFASLENEIPELTIQFLKEVGEIIARTIFNLRINQKTERLLQEAQQMTQELRENEEEL
                     RQNAEEMRATHDELEKSNQQLEAKIKEVENAQKRLHSLLENASEIISIYNSEKKLTYI
                     SPSVTKILGYSPQEMMSGKDFDRLTRKGESDFNEMFQQLIENPHIAITIQYTFMKKDG
                     EKIFLEVTGRNLLDDPAIGGIIINSRDITERKRAEKEERMRSKMQSLSENSPDIIMRI
                     NTQGQFFYANPMVEQYLGISYRDLVNQTLTALDISPTLSGFIKDAIKNIKVNKEKVEA
                     EITFASQMGETIMHIAAIPEFNENELETILFVGHDITEAKRIQMEIHDKNRKITESIN
                     YAQRIQTSILPNNRIIRQYLPKSFIYYHPRDVVSGDFPWFFTKDDYIYIAAVDCTGHG
                     VPGALLSFIGYFTLNNVVDHDSSYTAGEILDRLHYGVRKTLKQDRPDADARDGMDIAF
                     CKINPKTKELQYSGAHRPLYLMRNGELIEYKGNRKAIGGIPHPKKAEEDFVNHLIQIQ
                     PGDRIFFFSDGIVDQVGGPDKRKYSSQRIRELITSNPQTPIQDMAKVFSQDFDTYKGD
                     LKQVDDILLIGIEF"
     gene            9919..10494
                     /locus_tag="PK839_03345"
     CDS             9919..10494
                     /locus_tag="PK839_03345"
                     /inference="COORDINATES: protein motif:HMM:NF038262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SiaB family protein kinase"
                     /protein_id="HOG71804.1"
                     /translation="MDTKNVKGFLEFVYDFYKSMKAHEITLVYEGEITHQITKAFTSL
                     TESNMAKEEEANSVQKKVFHVMVECLQNISKHADSFGSDDFLFAGRGIFMVSKGDAEY
                     HVTTGNIIENSKIEELSQILDNINNLDKEGLKELYKTQMREGRLSEKGGAGLGFIDIA
                     RKTGRKLDYHFLPIDEDTHFFVLTSTISRTE"
     gene            10506..10889
                     /locus_tag="PK839_03350"
     CDS             10506..10889
                     /locus_tag="PK839_03350"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HOG71805.1"
                     /translation="METIKIMGTDDTPTVILDAENEIFEISGRSLPEDVTAFYDPVLS
                     WLDGYASSPNPKTIFTFKLVYFNTASSKLLLDVLMKLEQMHEAGHDVLVRWYFPEDDE
                     DMQEAGEEYADIVDVPFEQVSYTLS"
     gene            10980..14078
                     /locus_tag="PK839_03355"
     CDS             10980..14078
                     /locus_tag="PK839_03355"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOG71806.1"
                     /translation="MSEEILRALMQLFAIIAKQDEGVEMKERDYVETFLTQQLNDEAV
                     AEYLRLFDEHAGLLDKEIGSDDEERSKPKLTSVKDSVRILGICKKINKTLTQKQKIVV
                     LVRLYELINADRKFTEQRMAIINTVAEVFKLSHEEINDIENFVVRNNPEELDRPSILT
                     IHDRPFKGEQCKHIPTEALDGFIYILQIKSEDLYFLRYTGNEDIYLNGLPISNRRIYL
                     FASGSSLKLPKGKPVYYTDVVAHFLSDSTTIRISYRVDNASYTFKNGTVGLHNISFSE
                     GHGKLIGIMGASGAGKTTLLNVLAGIEKPTSGHVYINGYDLHASSNMLEGVIGVIPQD
                     DLLIEELTVFENLYYNAKLCFKDKTEPELIAMVDKTLSNLGLLEQKNLKVGTPLNKMI
                     SGGQRKRLNIALELIREPSIMFVDEPTSGLSSRDSENVMDLLRELTLRGKLIFVVIHQ
                     PSSDIYKMFDNMIILDTGGYMIYYGNPVEAVMYFKRLDMQINSEVGECPVCGNVNPEL
                     LFNIIEARVVDEFGRYTPIRKVSPQKWEEHFASNIKPPVIPDEKVEPPRTLNIPSRFK
                     QFTIYTMRDLRSKISNSQYIILNLLETPILAFILAYVVRYIADPNSKIYIFRENENIP
                     IYIFMALIVALFIGLTVSAEEIFRDRKILKREAFLNLSRSSYLLSKVAILFLLSAIQS
                     ATFILIANSILEIRDMYFDYWLVLFTTAAAANLLGLNISATFNSAVTIYIVIPLVMIP
                     MMVLSGAMFSFDKLNRSIGSFNRVPVIAELMITKWGYEALIVHQFKDNEFEKNFYEIE
                     KNLSNANFKVTYYIPELEKRATDALDLLQQEKSKANQEALKDKLAVIRKAVITETTVT
                     APSVPFAYTQKLTPEQFDEETAYALIDYLDALHRLYSDIYQTQNQKRDRIINYLVNTN
                     EKLYQAKRMAYHNESISDLATKVFEKNKILQYGDELVQQYDPVFRDPLPRHILDIRSH
                     FLAPRKHFLGMYFDTFWFNIIVNWIMSIILYITLYYESLKKLIELFSKIKVRGFFKAK
                     IS"
     gene            14125..15048
                     /locus_tag="PK839_03360"
     CDS             14125..15048
                     /locus_tag="PK839_03360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG71807.1"
                     /translation="MKSRFPIVIVAICSLSIALSSCKSCNKDKDNLGEELVIPDSLKW
                     DTPLPMGKPIVDELMQNISSPVETAALIKGLKIPFNKDYMASTKQEESFNTSFDKALA
                     LGIYGCDLGYLNMYEKTSFVVDYISVIKRFADDIQVGQFFDFETLKRLATNKENLDSL
                     ILISQQSFNRIDSYLRETNRSTLSAVIVAGVWVEGAYLTSRVAQTSNHPRIRETIGEQ
                     KTVLGFLLALLKAYSNDPNMAKLIAQLEPLKNQFDNVKITYELGEPEMIEVNGELTFI
                     QKDKQLVDMDDVTYKEIISGIQTLRNSLIKL"
     gene            15063..15503
                     /locus_tag="PK839_03365"
     CDS             15063..15503
                     /locus_tag="PK839_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG71808.1"
                     /translation="MKRILLAVAIIWVGLAFTARNANAQSEEELVQICGMIAGEAQYL
                     KDFRVKLDAGDPPPVQRIPILLKKDVKYRFTVCNSKDYEGKVVLQLFDNNRMLATTYV
                     IGTGKDYPSIDWVCTKTGAYHLFFSFRDGKAGLAVGLLSLVEMM"
     gene            complement(15599..16579)
                     /locus_tag="PK839_03370"
     CDS             complement(15599..16579)
                     /locus_tag="PK839_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008642658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine kinase"
                     /protein_id="HOG71809.1"
                     /translation="MPKVLGLGNALVDIMTQLPNDGLLETLKLPKGSMQLVDEGFMRK
                     AISFTRDMPQSLSAGGGAANTIHGLANMDVATSFIGKIGDDVFGRAFKTSMEEVGINP
                     IMLQGKAESGRTLALVSTDSERTFAVYLGAALELTAVDLKPEMFEGFDYFHVEGYLVQ
                     NHEMLLRALEIASAKGLQISFDLASYNVVEENRDFLNEIVKKYVTILFANEDEAKAFT
                     GKKPREAVEVLAEIVPIAVVKLGPSGSYIKRNSDFYEVGIIDVSSIDTTGAGDLYASG
                     FLYGLMKGLPLDKCGQIGAILSGHVIEVLGPKMDGERWTTIRRMVAEAET"
     gene            complement(16817..16893)
                     /locus_tag="PK839_03375"
     tRNA            complement(16817..16893)
                     /locus_tag="PK839_03375"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16857..16859),aa:Asn,seq:gtt)
     gene            complement(17085..17609)
                     /locus_tag="PK839_03380"
     CDS             complement(17085..17609)
                     /locus_tag="PK839_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HOG71810.1"
                     /translation="MDATRLTPDFYLRDVLLVAPELVGKRICRIYEDGTSSSHIVTEV
                     EAYKGMNDLACHASKGRTPRNDAMFQQGGILYIYFVYGMHWMLNVVASVENEPEAVLI
                     RGVAGCSGPGRVSRLLKIDRSFYAENLGSSQRIWIEDSGLFPSILTAPRIGVNYAGSY
                     WSSRPWRFYTNDYR"
     gene            complement(17657..18904)
                     /locus_tag="PK839_03385"
     CDS             complement(17657..18904)
                     /locus_tag="PK839_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HOG71811.1"
                     /translation="MVLIYNLGIRIYLLLIWLVSPVNPKAKSWLAGRRGLLDEVELLA
                     QNNDPKIWVHCSSLGEFEQGRPLIERIRESYPTLKVVLTFFSPSGYKVRKNYSGADYI
                     FYLPLDTKRNAKRFVEAVKPKMAIFVKYEFWYHFLSELHAREIPTYLISANFRETQIF
                     FKPYGRWYRKFLSHFTHIFVQNHLSLELLNSVGIKEVTVTGDTRFDRVSRVASESATI
                     PLLDTFCDGEKVIVAGSTWPKDEELLLNYLSANADRVKMIIAPHEISPSRVGSIVSAS
                     KVEAICFTRPEGTSPKQARLLVLDTIGFLSSTYRYGCLAYIGGGFGAGIHNTLEAAVF
                     GIPVVFGPNYLKFQEAMDLIALGAGFSIKSQAELNDLLDRLLNDPTLLSQAGESAGAY
                     VKSGVGSSEKILSIISNRLNKML"
     gene            complement(19020..19823)
                     /gene="lpxA"
                     /locus_tag="PK839_03390"
     CDS             complement(19020..19823)
                     /gene="lpxA"
                     /locus_tag="PK839_03390"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HOG71812.1"
                     /translation="MAQLNAYIHPDAKIGKDVTISPFSYIAGDVIIGEGTWIGPNVTI
                     MDGARIGKNCRIFPGAVISAIPQDLKFKGEVTTAEIGDNTTMRECVTINRGTASKGKT
                     IVGSSCLLMAYSHVAHDCILKNNIILGNGTQLGGEVEIDDFAIVSAHSLVHQFEKIGA
                     HVIIQGGSKVNKDIPPYIIAAREPITFAGINLVGLRRRSFTHQQIADIQEIYRIIYNK
                     GLNVSQALAFIIDNIPQTAERDLIVNFIKNSKRGVLREYTGPDPNEGID"
     gene            complement(19823..21220)
                     /locus_tag="PK839_03395"
     CDS             complement(19823..21220)
                     /locus_tag="PK839_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018696795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase/3-hydroxyacyl-ACP
                     dehydratase"
                     /protein_id="HOG71813.1"
                     /translation="MADKQRTISQPVTLTGKGLHTGVDVEVTICPAEVNTGVNFQRTD
                     LEGQPKIEAVADNVIDTSRGTSIGTKNVKVNTIEHMVAALYGMGIDNALVKVNGPEMP
                     IMDGSSRFFVEAIASVGTVEQGAIRNYFTIKEKMVFSDEKNGIEIVAYPDDGFSIDLM
                     VDYNSRVLGHQYAKMGNIDEFASQIAPCRTFVFFHELEVLLKHNLIKGGDLENAIVII
                     EHEVPQPELDRIADLFNKPHVKVKPEGILNNLDLHFDNEPARHKLLDMVGDLSLVGVR
                     IKGKIIAIRTGHWANTEFAKILRKQYKKELLKPSAPEYNPNVPPLMDINQIKKTLPHR
                     PPFLLVDKIISMNGTSVVGLKNVTMNEAFFTGHFPHEPVMPGVLQIEAMAQVGGILVL
                     NTVPDPENYTTYFLKIDQVRFKHKVVPGDTIIFRLTLNEPIRRGIANMTGQAFVGNQL
                     VMEGVLMAQIIKNPN"
     gene            complement(21329..22375)
                     /gene="lpxD"
                     /locus_tag="PK839_03400"
     CDS             complement(21329..22375)
                     /gene="lpxD"
                     /locus_tag="PK839_03400"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005864351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HOG71814.1"
                     /translation="MEFTAQVIANFLGGVVEGNPEVKVSTVSKIEEGSPGSLAFLANP
                     KYTPYIYTTNASIVLVNADFVAEQPVKATLVRVKNAYEAFASLLQLYAQQTEPQLSGV
                     ETPSFIHPSAAYGDDFYLGAFAYISGDVRIGSRVKIYPQVFVGKGVIIGDNTTIYPGA
                     VIYHECTIGSNCIIHAGAVIGADGFGFASQEDANYKKIPQIGNVVLEDYVEIGANATV
                     DRATMGSTILHRGVKIDDHVHIAHNVEVGENTVMAAQCGVAGSTKIGKNCMFGGQVGA
                     SPHIKIADGVMAGAQSGIAGDIRKPGTIILGSPAFDVSKQRRSMAVYKNLPDMANKLN
                     EMQRTIEELKKKLL"
     gene            complement(22412..23668)
                     /locus_tag="PK839_03405"
     CDS             complement(22412..23668)
                     /locus_tag="PK839_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005823703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOG71815.1"
                     /translation="MRNDKINKNKIVNDPVHGFISINYPLIFDIIEHPYFQRLRGIKQ
                     LGLTYLVYPGANHSRLQHALGAMHLLDTAIDTLRSKGFVISEEEAEAACAAILLHDIG
                     HGPFSHTLENTIVPGVSHESISLAFIRSLNEEFNGRLSLALEIFEDRYHRKFLHQLIS
                     GQLDMDRMDYLKRDSFFSGVVEGSISTERIIKMLNVVDDNLVVEAKGIYSIENFLIAR
                     RLMYWQVYLHKTVVAAEQTLVKLLQRAKQLVRSGFPMNISAPLLYFFAHDVTGDDFFT
                     PQSKALSYFAMLDDSDIMVAAKDWMNCSDPVLAKLSSMIINRRLLSIELATIPSSSSR
                     LAELKGLFSSAFPNFTEYTDYFVFSDSVGISAYQPGEESIQILHSNGQLVDIAEASDI
                     LKSNALSNETKKYFTCFPKELREILR"
     gene            complement(23824..24393)
                     /gene="ruvC"
                     /locus_tag="PK839_03410"
     CDS             complement(23824..24393)
                     /gene="ruvC"
                     /locus_tag="PK839_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HOG71816.1"
                     /translation="MTNSTPAEKIILGIDPGTNILGYGIVRCSGKNDLKLVVLGVIEL
                     KKYEDHYLKLKAIYDRVQQLADQYLPDEVAIEAPFYGKNVQSMLKLGRAQGVAMAAVL
                     SRSIPIFEYAPRKIKQAITGSGSASKEQVAAMLKSILGEKDFSQEYLDATDGLAAAVC
                     HFFQGATVGASRKASSWSDFVRQNPGRVK"
     gene            complement(24502..25761)
                     /locus_tag="PK839_03415"
     CDS             complement(24502..25761)
                     /locus_tag="PK839_03415"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOG71817.1"
                     /translation="MNWHKILSLAVIAAMVLSSCDNEDEQKVNVNQEVYNLMDDMYYW
                     YDQMPEVDPNAYKDPVELVNALRVYPPDRWSYVTTKAEFDAYYNQGAYVGFGFGSGFS
                     ANNELYITFVFKNSPLAAMGIDRGWQIISIDGQTPTSENFSSLIGPSEVGVSKKFLFK
                     SPEGSTMEQIFTKSQIEMNSVLMDSIYTTGTFRVGYFVLESFIGPTEAELTGLFSKFK
                     EEGINELVVDLRYNGGGEVGVAKYLASLIAGSSAFGKVFGTYYHNDKHQGDNSSITFT
                     YEDNSIPLNRVVFITTSGTASASELVINGLKPHIEVSLVGSKTHGKPVGMYSFEFRDP
                     SIDWVIVPICFSIRNSNNEGDYFDGIPVDVSAADDITHPFGSMDEESLNAAFTHLGVT
                     AKGYTRKAAVGVQPFVGKGLQGEIGAW"
     gene            complement(25855..26772)
                     /locus_tag="PK839_03420"
     CDS             complement(25855..26772)
                     /locus_tag="PK839_03420"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOG71818.1"
                     /translation="MTLKSQNRAYVYASLVVLFWGTSATAFKIGLSYLSTVQLLFWSS
                     LAATTILFLVLCSQHKFKQLSAATPAQLGRSVMLGFLNPFLYYLVLFKAYSLLPAQVA
                     QPLNYIWPIVLVLLAAIFLGQKLHWYDLLALLVSFTGVTLISSQGSINIFSRSNPLGV
                     VLALSSSIVWATFWIVNMQKNAGDEVVTLFLSFASASIFCLIAMVTLGGFAKPLPWQG
                     ILASAYIGAFEMGITFVLWLKALNLATSTAKLGNFSYLVPFVALIFISLILNEKIIWT
                     TVAGLICIIGAILFQQYLGRKLDNEGQKP"
     gene            27019..27528
                     /locus_tag="PK839_03425"
     CDS             27019..27528
                     /locus_tag="PK839_03425"
                     /inference="COORDINATES: protein motif:HMM:NF025426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4252 domain-containing protein"
                     /protein_id="HOG71819.1"
                     /translation="MKQLAILLVTLVFPLVLEAQGIAEEMFERYAGKPGYTSVAIGSE
                     FLNFVAALDREDNELQDLSNKISSLKILVAKQQVEEFGKTLERGITREGYKEVMQAND
                     EGGLVTFYALPKGDKFSNFILHVTNSDEEVMLAIAGNFTLSDLSKLGGSSEDDDSYIS
                     LLRKLENEK"
     gene            complement(27583..27903)
                     /locus_tag="PK839_03430"
     CDS             complement(27583..27903)
                     /locus_tag="PK839_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005468277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HOG71820.1"
                     /translation="MESRNELAIQSDIVKVLKGVFDPEIPVNIYDLGLIYEVDVDSNS
                     KVTITMTLTAPNCPLADDVVDEVHEKVSAIEGVSGVVVNLTFDPPWDKSRMSDEAMLE
                     LGFL"
     gene            complement(27988..29202)
                     /locus_tag="PK839_03435"
     CDS             complement(27988..29202)
                     /locus_tag="PK839_03435"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005927282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HOG71821.1"
                     /translation="MNVEKIRQDFPILSREVYGMPLVYLDNAATTQKPKAVLDRVAYF
                     YNQINGNIHRGVHKLSEESTQEYENAREVVRNFINARKTSEVVFTSGTTASINLAAYS
                     FGEVFVHSGDEVVVSEMEHHSNIVPWQLLCERKGASLKILPFRDDGQLCIDQLEGLLT
                     PRTKIIAVTQVSNTLGTVNPVKEIVRIAHNHGIPVLVDGAQGVKHGNIDVQDIDADFY
                     AFSGHKIYGPTGIGVLYGKENWLEKMVPWQGGGDMINRVTFAKTTFNELPFKFEAGTA
                     NYIGASGLAAALDYYTKIGVSAATAYESDLIQYATRKLSEIGGIRIFGTAPDKAAIIS
                     FLVDNVHPYDTGMILDKLGVAVRTGNHCTQPVIDHYGIDGTVRASLAFYNTYDEVDRL
                     VEAVQRVKEIFG"
     gene            complement(29349..30713)
                     /gene="sufD"
                     /locus_tag="PK839_03440"
     CDS             complement(29349..30713)
                     /gene="sufD"
                     /locus_tag="PK839_03440"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HOG71822.1"
                     /translation="MSKTDTAGLLDDLVSLYLDNHDLITGGSSDTLNKHREEAIQNFR
                     IIGLPSAKNEDYKYSRVEPLFQHEYEKYFTPKKISFRVDDIFRCDIPELDTHLAMVLN
                     GFYLPSSGQLQKLDNGIIVGSLAQAAKDFPEIVAKHYNVYAQCNADGLVALNTAFAQD
                     GIFIYVPKGAVEEKPIQIINLLMSEENLMVNYRNLIILEESSHANILVCDHTLSSKRY
                     LSNVVTEIFTGANSTLEYVRMQNEHNDSSQLSHTFINQEEGSSVKTNTLTLHGGFIRN
                     NLHIALKGEGASAQAFGLYLTDRTQHTDNFTFIDHAVPNCSSFEHYKGILDEQATGAF
                     NGKILVRPNAQKTQAYQSNNNLLLTADARINSKPQLEIYADDVKCSHGATVGQIDADA
                     LFYMRSRGIGERDAKLMLMFGFAHEIVGQISIPTLRDRLDELVDKRLHGELSRCHTCP
                     MHCC"
     gene            complement(30730..31485)
                     /gene="sufC"
                     /locus_tag="PK839_03445"
     CDS             complement(30730..31485)
                     /gene="sufC"
                     /locus_tag="PK839_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004307398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HOG71823.1"
                     /translation="MLIVNDLHARINDKEILKGINLEVKAGEVHAIMGPNGSGKSTLA
                     SVLTGREMVEVTHGQVTFNGKNLLEMPPEVRSREGIFLSFQYPVEIPGVSMVNFMKAA
                     VNEQRKYRGLEPLSATDFLKLMRERKELVEIDSALTNRSVNEGFSGGEKKKNEIFQMA
                     MLEPKLAILDETDSGLDIDALRIVANGVNKLKSPDNAVIVITHYQRLLDYIVPDFVHI
                     LYNGQIVKSAGKELALELEEKGYDWIKNGNLKG"
     gene            complement(31671..33122)
                     /gene="sufB"
                     /locus_tag="PK839_03450"
     CDS             complement(31671..33122)
                     /gene="sufB"
                     /locus_tag="PK839_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HOG71824.1"
                     /translation="MNGEQDKILHEVTGSEYKYGFVTQIDTETIPRGLSEEVVRMISS
                     KKGEPDWMLEFRLKAFRHWQTMEMPTWAHLTIPPIDYQDIIFYAAPRKRNASTGGGGE
                     IDPELKATFDKLGIPLEEQQMLSGVAVDAVMDSTSVKTTFRDTLAEHGIIFCSMSEAI
                     REHPDLVRKYLASVVPSTDNFFASLNSAVFSDGSFCYIPKGVRCPMELSTYFRINAAN
                     TGQFERTLIVADDDSYVSYLEGCTAPMRDENQLHAAVVEIVALENAEVKYSTVQNWYP
                     GDKNGKGGIFNFVTKRGICKGKNSKISWTQVETGSAITWKYPSCILMGDNSYGEFYSV
                     AVTNHHQQADTGTKMIHIGKNTRSRIVSKGISSGEGSNSYRGLVKVLKGADNARNFSQ
                     CDSLLIGDRCGAHTFPYIEVDNPTAIAEHEATTSKIGEDQLFYCNQRGISTEDAVGLI
                     INGYAKEVLNKLPMEFAVEAQKLLQVSLEGSVG"
     gene            33931..35877
                     /locus_tag="PK839_03455"
     CDS             33931..35877
                     /locus_tag="PK839_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018463067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HOG71825.1"
                     /translation="MRNDKAGKISKLSFVGLIITMGIVYGDIGTSPLYVMRAIASSVT
                     TIDNDFILGALSCIIWTLTLQTTVKYVLITLRADNKGEGGIYSLFALVRRKMPKAYII
                     AIIGGSTLLADGIITPAITVTSAIEGLQIVQKQLPTIPIVLIIITGLFVIQQFGTKKI
                     GSSFGPIMTLWFLTLAILGVNQIVQLPVVFKAFNPYYALQLIVEHPSAMLVLGAVFLC
                     ITGVEALYSDLGHCGLKNIQISWIFVKSALILNYLGQGAYLLKNGFNGNNPFFSIMPQ
                     WFVLPGIILATAAAIIASQALISGSFTLISVASSLNLWPNIKRKYPTNIKGQIYIPSV
                     NWFLFLGVIFVVLFFQESSNMEAAYGLSVNLTLISTTILVAFYLMSKKVNPVLITLFS
                     LSFLLIEGVFLYANLLKFKHGGWLTLTLASLFFIVKYGWYNARKIRNRHITFTSINEY
                     LPIICDLSKDKEVPKYATNLVYMTRANNPDEIESKIIYSILNKQPKRADTYWFLHVDK
                     LDEPDTFAYEVTEMVPGIIRRIDFRLGFKIEPRVNLYFHQVLEEMTALKEIDLVSHYK
                     SLRKYNITADFLFIMIDRVPNYDIDFSPFEKFMMVLYSIFRRLGISEVKSLGLDSTNI
                     IVEKVPLSTHVSYKRKAIKRKIFS"
     gene            complement(35951..37150)
                     /locus_tag="PK839_03460"
     CDS             complement(35951..37150)
                     /locus_tag="PK839_03460"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOG71826.1"
                     /translation="MHRFSANYIFPVSQPPIRNGVVEVDDDGVIMRVFHPGNSPKEIH
                     STEFYNGVIVPGFVNAHCHLELSHLRGKIPKNLGIAGFIKAITEQRGTDEEVQNHGEL
                     VERAIAGAIHEMEQTGTVAVGDISNSADTLLAKLQSDIYFHNFIEVFGLNPNEAQSIF
                     EGYLEVYEVFKQKFCGSTSLTPHSPYSISQPLWEKISEFIGKSKELVSIHYAESAAEY
                     QFLQDGTGPLSDRYQSAGIPFCKPQGLTPRGVVSRYVNRDCKVMLIHNTFAPAEELSW
                     LAAIYPQTTVVTCPESNLLIEGKLPNLPEFETSGLNIAIGTDSLASSSSLSMLSQINI
                     ILQHFPQLTFSNVLQWATLNGAEALGISSTYGSLEPGKNPGLVLITGFDFRNMCATPQ
                     SLAKRLI"
     gene            complement(37152..37997)
                     /locus_tag="PK839_03465"
     CDS             complement(37152..37997)
                     /locus_tag="PK839_03465"
                     /inference="COORDINATES: protein motif:HMM:NF025494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4296 domain-containing protein"
                     /protein_id="HOG71827.1"
                     /translation="MRKMVIWLVGLPAVVIACLSCGNSGIIPENDMVKILSKIYITDG
                     ITFLPEKHTLFGRDTIAYYEPIIESYGYTTQEFDSSMKYYAYHTEKLDEIFDRVIMEL
                     SKMEEQASINPKNLEEDQNTPTDTSENLWPHKTFWNMAVDYGQNRSLGFDIPVKGLGD
                     YTLSFEAQLFPDDSSVECRMNAFWFIADNVQRDYRDVAKVQLYPKDGVVHHYSFTFSC
                     TDSQYTHLKGWLYDNANKNQNFNRHAVFNNIKVTYKPAPKSPVRNLKDKKPIKAFRKP
                     IPVQQ"
     gene            complement(38094..39035)
                     /locus_tag="PK839_03470"
     CDS             complement(38094..39035)
                     /locus_tag="PK839_03470"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HOG71828.1"
                     /translation="MKTTRTAAYLVYPLVTVIMLGMAGQVNSQQIPHYSQYMFNNYLT
                     NPAVAGTLNHYQMRLNNRYQWVGLNDAPYTVNMSFYGPFTKHDMGWGGYFSNDVTGPT
                     SRTSIMGTYAYNMQVSQSGMRISGGISFGMMIYKLDGSKLTLGEEPDPMYDPAIIQAT
                     KSVFTPDASVGFYLYSTSFNVGLSAHQLFGQKLKFYPEPIGVNRLKQHIMLSGGYWVN
                     LNRYMELETSAIIKYMFASPMQFELNGKISYRQKEYQFWGGLSLRWKDAVSALVGINW
                     NRKFMFGYSFDWSLLGISKYNSGSHEIMLGYMFDKIK"
     gene            complement(39195..45110)
                     /locus_tag="PK839_03475"
     CDS             complement(39195..45110)
                     /locus_tag="PK839_03475"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOG71829.1"
                     /translation="MLYSNIITITIIPEIANNTISANQTICFGSVPVPLTGSLPTGGS
                     GIFSYQWQSSTVGATGPFSNIPGATSQNYSPTALSQNTWYRRVVTSSSCVSFSNVVAI
                     TVLPSVSNNTIAADQTICYNTTPELLTGSIPTGGNGTYSYQWQSSTTGAAGPFFDISG
                     ANAESYQPGSLTADTWFRRAVSSGPCTDYFSNVVKITVNAEFSVSGFSSSSPTCNGYS
                     NGSATVNLSGGAPPFSYSWNTTPEQTTQTATGLSAGVSYSVTITDNLGCEAAGNVTLT
                     QPDAIGLASKTVTPVTGCNGNSNGSIEALGSGGTPDYTYSLYDGPSLIGTQSPSYPTP
                     ASFASLPASTYTLSITDANSCPAYTEDIVITEPDPIIITDVSTVPISCFGAADGVITI
                     TASGGTGSLDYSIDNGVTFQASNTFTVGGGYYDVVVRDANGCTASWPATIEMVEPSEI
                     SFTYEVAAISSCYGDHTGEILISNVAGGSGTGYEFSIYVPEVWGSDPHFTGLPGGPAN
                     PYYIKVRDSHGCISVANNGNPIYISQPTPIAFTVNTTDVTGCWYNTNGKIWVTGVSGG
                     TGSKTVSIDGISYFPTTHTFNVGVGSYTVYVKDSKGCIVTKPAVILGPPEIVIDNLTV
                     TDASCFGAADGEVSAIASGGTGTLEYSIDGITYQATGDFSGLTADTYTLYVRDANGCV
                     LTQEFTVGQPDALYFTTRVKTDISCFGANDGTITLEVAGGTPPYNYSITGGAPFGNAT
                     GLFTGLSANTYIPAVLDANGCSVIGMSLTIVEPALLAISSQTSTNITCFGANDGTITV
                     EATGGTAPLTFSLLDASLIVIATNETGLFTNLAPGTYTVEVNDANSCGPVSAGPFIIT
                     EPSQLTFTYSVVDLVCNGDANGQITINASGGTPPYDYSYDGGLNFEVNNVKNGLSGGD
                     YTVVVRDANGCTDTQVVSVYEPAPIILSLATSNISCGGLTDGSIDASATGGTLPYQYR
                     IDGGAWQPTGSFTGLSASTYLVEVIDASGCITGENATITEPPAIVISSVTPVNPTCTT
                     TGSITVTASGGTGILTYTLMPDNVSNTSGLFMGLGAGTYTVDVTDANGCGPVSTAPIT
                     LTSPSTISIDNVIVTHVTGCYGDTNGSLEIIASGGTPPLVYSIDGGATYHPSNIFINL
                     PANDYSIVVNDVNGCLQSTLRTINQPAQITIDNVVVVQESTPGANDGEIHVTATGGTG
                     ALTYTLQPNGISNGTGNFTGLGTGTYHVDVMDANGCMVQTGDLIISGVNINITSNNVS
                     CHGGSDGLITITITGGFPPFTVACNEIDMTPIPVNDLGGGVFSAEGLSAGSYLITVTD
                     NVPHTFPTYQIDILEPDSLIATFVSLQNPQCHGGADGSVLFNITGGTPVYTITWDGGT
                     SVGTTATPVTAGYHEFTITDQHGCSTVVSDVPELVEPNPIQLVEQTQFDPTCHGEAIG
                     IISVTATGGTGTLTYNLSGTATLSNTTGVFENLFAGVYDLSVTDDNGCPDNFDPPVRF
                     TLNEPPQIIISGLTPDDSLICPNDPSGFVRVQVSGGQPDYTYLWSNGQTTVDLDSVVV
                     GDYTITVTDALNCRVSQTFTVRGTPMLDLNPVVIDTALCRIKPTGNTGRIDIRNSTGG
                     NGQFADLEFQWNDANATTGPTLPKAGDPMIIGGGIYTVTVKDTSGCVYSFDYEVPINP
                     RYNFLTNAGSDTTVCYSNPVILHGSVISGNEGGTVDTTFDFSYEWRKFPDIDGDPIHR
                     GQDYQVNLVESSSFLLWAIENNSGCKDSTRINLNVLPKIGLEVPLYISAVQDTIISIL
                     TGKEVNLDVITRSVEYATEFQWKPAEMFNPPDSWNSSILINDQIKQQIPEERKQKLTD
                     PISKKPTDFILVDVIATTEMGCTDSLRLYTKEVTSLGFANVFSPNGDGINDVWGVPKD
                     YLFPDLSIEIFNRWGSLVWSATGDKAAKGWNGRTSNGNELPIGTYYYIVKFNVQAQGA
                     SWKPITGSVTIVK"
     assembly_gap    45191..45290
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<45291..47098)
                     /locus_tag="PK839_03480"
     CDS             complement(<45291..47098)
                     /locus_tag="PK839_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG71830.1"
                     /translation="MKTFTKNQTLPKCLSALLFVLMFTSGMAYSQFSVTIKTLPASDP
                     PTVCQNSSLVLKAYPTGGVTPYGPYTWGGAPFITPAGDIAVLTPDETTTPGIYTISCT
                     VKDHVGNEVTGYIDITVYESPTASITNNGTTTFCQGGSVELEAYSEAGYSFQWRRDDA
                     DISGATSSTYNATVSGSYNVVVTNGNGCDSYSNYIYVTVNPLPSATASNDGPACYNGT
                     INLTSGPNGMVSYSWTSTSTTPFSSTLQNPSITNVTPDNSGDYTVTVTDANGCENSAT
                     TTVLVYANLNGGTVGSDQTICYNGDPASFSNIASPSGGDGVWTYGWEYKVGAGPWNSI
                     SGATGLTYDVPAGLTQTTQYRRVATNGCGTVYSNTITVTVYADLNGGLISSSQSICYN
                     GDPAAFSNDAAASGGDGTFTYSWESKVGAGSWTPILGATSSTYDVPAGLTQTTLYRRK
                     ATNTCGVAYSNELTVTVFGALSGGVVAADQSICYSGDPAAFTNVTSPAGGTGAWTYSW
                     ESKVGAGAWTPIGGASGLTYDVPAGLTQTTMYRRAATNACGTVYSNVLTITVYAQMNG
                     GVISGNQSLCYNSDPSPITSTTNPSGGNGTWSYSWE"
     gene            complement(47694..48299)
                     /locus_tag="PK839_03485"
     CDS             complement(47694..48299)
                     /locus_tag="PK839_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG71831.1"
                     /translation="MTFKPDRWLSILLHPLFMMCYAFLTLYLSASYISFLPDSYKRLL
                     FIALFLNTIIIPIFILMLLKRLGTVKSIELFHHRERVFPIVMVIIPYVFTLLLLIRLQ
                     VPEIFIKIVQGGILAMIIAATVSYWWKISLHMVGIGGVTGFIIAGLILHYFTSLNLLV
                     VVVVLSGLLASARLVRGDHCPSQVYTGYLTGLASILLVFLL"
     gene            complement(48309..49778)
                     /gene="rpoN"
                     /locus_tag="PK839_03490"
     CDS             complement(48309..49778)
                     /gene="rpoN"
                     /locus_tag="PK839_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005860047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HOG71832.1"
                     /translation="MLKQRLQQKLLQKLSPQQIQMIKLLEVPTMLLEQRIKKEIEENP
                     VLEEVHDAEEPENDTEASLVNDDSDTETDKNDDEFTLEDYLNDEDIPNYRLVASNVSK
                     DDKREEIPFSVGVTFQESLKSQLGLTSLNDREKQLALYLIGNIDEDGYLRRKLSAIAD
                     DVAFALGLDTNEDELEKILYVIQEFDPPGVGARDLQECLLLQLRVKENGSTTASTAYK
                     ILKYHFDEFTRKHYDKIAQKLSLSDEQLRDAINEILKLNPKPGGGFSDPYNQSIQQVI
                     PDFILDYVDGEFQLNLNSRNVPELRISREYSDMLESYSYSKDSATSQEKEAVTFVRQK
                     IESARWFIDALKQRQNTLMTTMRAILNYQQEFFRDGDESKLKPMILKNIADATGLDIS
                     TISRVANSKYIQTHFGIYPLKYFFSEGLMNDAGEEVSTREIKKILQECILNEDKGNPL
                     TDEELMEALKQKGYPIARRTIAKYREQLGIPVARLRKQL"
     gene            complement(49785..51182)
                     /gene="asnS"
                     /locus_tag="PK839_03495"
     CDS             complement(49785..51182)
                     /gene="asnS"
                     /locus_tag="PK839_03495"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HOG71833.1"
                     /translation="MRNSERVKVKNLLESGTVGAEVAAMGWVRTKRGNKSITFIALND
                     GSTINSIQVVFDMAVFSEDQLKNVATGACIGVRGKLVESPGSGQRVEIQASYLEVLGE
                     ADSATYPLQKKGHSMEFLREIAHLRPRTNTFGAVLRIRHALAFAIHKYFNDRGFIYLH
                     TPIITGSDAEGAGAMFRVTTLDPNNLPRTPEGKIDWSEDFFGKEAKLTVSGQLEAELG
                     AMALSEVYTFGPTFRAENSNTPRHLAEFWMIEPEMAFYDIQDNMDLAEDFLKYLIRYV
                     LTSCADDLEFLKKMYDDELVSRLQLVVDNKFERLTYTRAVEILEKANQKWEFPVFWGA
                     DLQSEHERYLVEKHFSKPVILTDYPKEIKAFYMKQNDDGKTVRAMDVLFPKIGEIIGG
                     SQREENYQKLLSRITELGVPEKDVWWYLDTRKFGTAPHSGFGLGFERLVLFVTGMSNI
                     RDVIPFPRTPKNCEF"
     gene            51557..51940
                     /locus_tag="PK839_03500"
     CDS             51557..51940
                     /locus_tag="PK839_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES family protein"
                     /protein_id="HOG71834.1"
                     /translation="MSLSFNPNAFDNLIVVGDRVLIKPKNPDERTKAGLLLPPGVQEK
                     QKAHSGYVVKVGPGYPIPTIGELDEPWKKSRENARYFPLQAQVGDLAVYLHENSIEVE
                     FNNEKYVVVSFSSILLLFRDEGLLE"
     gene            52190..52810
                     /locus_tag="PK839_03505"
     CDS             52190..52810
                     /locus_tag="PK839_03505"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOG71835.1"
                     /translation="MKKSTLIFNIILSLAVVALFILHFTQKSPRNHVSGSTPSEASAA
                     LSEGLSVAWVNMDTLLNHYDMYFDMRKELENNSRNLEAQLNSKSKAFEQDAMDFQNKV
                     QKGLVTRSEAQQLQTSLANREQELYKLRDDMQMQLAEEEQVKLRQIHYSITEYLKKYN
                     ADKGYHIILSSNFGGPLLYGHPALDITSEVIGGINQEYAANHKTDK"
     gene            52857..54170
                     /locus_tag="PK839_03510"
     CDS             52857..54170
                     /locus_tag="PK839_03510"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HOG71836.1"
                     /translation="MGNHLKGFRHFTTMEIPEYFQNKNFSPSIQELPTKKLSIVVVIP
                     SFNEADLKDSLMALASCSKPRGDVEVIVVVNHPENSSAEVVTTSEQSLQVVAEADSCW
                     GSSSFRFYAIKAFNLPQKQAGVGLARQIGMDEAAYRLFLAKQPGGIIACFDADATCEV
                     NYLVELENFWAANPDIHACSIRFEHPIQGNNFPQEVYDGIAQYELHLRYYNQALRYTG
                     FPFAFHTVGSSMACTAEAYVKFGGMNRHQAGEDFYFLQKIIPHGHFAELNTTCIYPSP
                     RASNRVPFGTGKSMAMFLENPGNEIQTYQLESFFPLQMLFGKMQSLYQLDVGQIDCFI
                     DSMPAPLKLFLKQNNATEKIYSISRNTASPGSFRKRFFLWFDAFLMLKYLNFVHNRFF
                     KRQPVKHEAIRLAGIIGLRVDERWPVEKILVKYRELEANKFNVNP"
BASE COUNT        15232 a        12731 c        11965 g        14483 t
ORIGIN      
        1 tgtagaggtt gaaatatagt aactttacac ctttaagtaa ttttgcctta aaatgtttgg
       61 tagaatacta aaaacaatcg ggaaagtagg gagttttctc ataatttttc ttgttttctt
      121 cctcctctac tcctcaatca cctacttcga tccagaaagt agggttgtgc tttggcagtc
      181 ggacaagccc gatacaattc cattcgattc caccctggcg gcactaactt ggaatatagg
      241 ctatgcagga ttaggcagca acatggactt ctttatggat ggagggggaa aaatgcggga
      301 tacgcgtgaa cgaacaaccg agaatttaac cgcaattgag aaaaccctag gcggtgaaaa
      361 aatcaacgac ttcatcctgc ttcaggaagt tgatattaac tcccatcgct cgtacggaat
      421 aaacgagctg gaggaaattt caagaacttt agaatacccg tactctatag gcattaacta
      481 tcgggttaat tttgttcccg taccccttaa aaaccctatg ggaaaggtgc agtcgggcat
      541 tgtaaccttc agcaagttta accctaagga aacgataagg tatgcgtatc ctggggcatt
      601 cccctggcca aacaggctct tcaacctgcg gcgttgcatg gtagtgaacc gctaccccac
      661 aagcaatggt aaggattttg ttctcgtcaa tactcataac tccgcattcg acaatggcgc
      721 acttaaacaa caggaaatgg atttcctgca agaaatttta ctaagcgagt actacaaggg
      781 caactacgtg gtggccggtg gcgattggaa ccagcttccg cctggagcac catatgctga
      841 acttgttgta ggtagcagca actcttcgtt catgctaacc ggtatagaca gtagctttat
      901 gcctaaagac tggtcatggg tatttgaccc ttcgtgcccc accaaccggt acttaaacga
      961 accctacact ccaggccgca ccaatacgtg tattctcgat ttctttttgg tttcacccaa
     1021 cgtgagtgtt cagcgctgcc agacagccaa catggctttc aagaattccg atcataaccc
     1081 ggtaaccctt gagtttaggt taataggcaa tccataatac attaaccaaa tgcagccgca
     1141 cgggcttgac gttaatcaaa aacagcaact aaccggttaa gtaattagtt ggactcctta
     1201 tcacaaacag ctatcaagaa ttcagaccaa aattctaaca aatcctataa ctccatgttg
     1261 ccttggtcaa caggtaaggc aacatgaaat ttaaaaatca gtcgtttgtc aaagtagctg
     1321 tttcccctct tcttcgtatt tttggaattt attcaaatcg ctataacttt cacgacgctt
     1381 tgtcaattta caaactatac ttacctttaa cagggaaaaa gggatgcagt ttggcaggcg
     1441 tactaaaacg tttttaccaa aaatggagtt attaagttga agcacatgca ggtaaccgat
     1501 caggatttgc aatactttat cactacgctt aaaaatgtat caaagtacga tttttccgag
     1561 tactcggata aatcgctgaa gcggcgcttg cagaaaattc tggaggatta caacctggat
     1621 atattcagcc taatcgccaa ccttaaaaac gacagggcat tcattgaaaa agtggtaaag
     1681 gaagttaccg tgaataccac agagcttttt cgcgatcccc cggtgtggca cctcttacgc
     1741 tcccgaatac tcccgcgctt tagaaacaac aaaaccataa acatttggca tgctggttgc
     1801 tccaccgggc aagaggttta ctccatgatg atactgctca acgaaatggg tatgttggaa
     1861 caggctaaaa tttttgccac cgatataaat attgacgtgc tggatgctgc aaaacagggg
     1921 gtttacaagt accgtttcaa cctgggctac ctggacaact tcgacaaggt gatcaagcaa
     1981 aaccccttga actttgaaga gtacaacgag gttccctacg aaaagtactt tgaaattgac
     2041 aaggtaaagg ataccataac catgaacaaa ttcctgtcgg aaaaaccaat tttccgtaag
     2101 cacgacctgg taaccgacgg gaatattttc tttgccaagt tcgacctcat cctttgcaga
     2161 aatgtgatta tctacttcaa ctactccctt cagaacaagg tatttgaact atttcactca
     2221 aacctttacc ccaagggagt gctgctgctt ggaatgcacg agaccatact tggcccctgg
     2281 gccactaagt ttgaaaaact tggacaaagc tatgtgaaaa aatagttatg aagcaacctt
     2341 ttgataccga caatttcaac accgctgatt gcacaattgg caacgtacta tgtagaatac
     2401 ttttttttgg tcagaggcaa gctggtggaa aacttatcat cacgttaagt aagcttttag
     2461 ggaaaaatgt aacgaattgt aactttaaat tctacatgaa atgaaaattt cacaacgtat
     2521 tcagctcatt atcgttccac tgatgatcct gttgttttcc gcttccggag taacgctgaa
     2581 ctacctgtca aaaaataggg ttagggaaag catacgaatt caggaaggag tgtacataga
     2641 caagctgata caaattgtcg atatggtgga agcaaaaaca ggaagcggcc tatcctccga
     2701 tgatattttc gagctcagga cttacttcct ctcgccagcc tactatagta ccgattaccc
     2761 cgccattatc tcaaccaatg gcgtttacct gcttcacaca acacttcaag ggcaaagact
     2821 tccctctgct gcaactgccc agattagaaa gagcaaaagc accgacggct acatatatta
     2881 tgacgattat tcaggaaaca aacccgagaa gcggttgcta tgctacaagt tctataagcc
     2941 ctacaatgcc tacattgcca tctcggtcag tatgaacgaa gccttggctc caatgaaatc
     3001 caaccgcatg gcaattatag tatttatcat tctggcgtcc cttctaacag cctttgcaat
     3061 tcctttactc cttaaaccca tcatacgcat tataaacctg gtgaacataa agctccgcca
     3121 gctggcaaaa ggcgacattc caaaccccat caaaataaca tcaaaggatg agcttggtga
     3181 aatggttacc tcgctaaacc agcttatgga agggctaaag tcaacaacca actttgcaca
     3241 tcaaattgga cagaaccagc tggagcacac cttccaacct ctgagcagca aggatgaact
     3301 tggcaacgcc ctgcttgata tccaatcaaa cctgaagcat gccctggagg aagaacagcg
     3361 cagaaaggag gaagactaca tccggggctg ggtaaacgaa ggtcttgcaa agtttggaga
     3421 tatcctgcgt caaaacaaca acaacctaac cctattggcc gacagcatca ttcaaaatct
     3481 ggtcaactac ctcgaggcaa accagggggg cattttcatt tacaacgatt cagaaaacga
     3541 caagcacctc gagatgcttt ctgcatttgc ctataaccgg aaaaaattca tcagtaaaac
     3601 catccccctt ggcgaaggac ttgttggtgc ctgcgctctt gagaaaaaga ccatctacct
     3661 caaggagata cctgctgact acattgccat aacttccggc ctaggagagg ccacccctac
     3721 cgcactgcta atcgttccac ttaaacttga ggatgaaatt tttggagtag ttgaggtggc
     3781 ctcattcagg gaatttaaac cttacgaaat tgagtttgtt gaaaaaatag gcgagagcat
     3841 cgcttcaacc ctctttgccg ttaaaaacag catacgaacc aacatgcttc tcgagcaatc
     3901 gcaacagcaa agggaggaga tggccgcaca agaggaagag atgcgccaaa acatggagga
     3961 aatgcaggcc acccaggagg aaatgctacg gaaaaacatt gagttggagc tcattaccaa
     4021 taccattaac cagtcgctac tttctcttac cctttccgaa gaaggctaca tagcagactc
     4081 caaccaaaat tttgtggaaa cccttgggta ctccaaaacc gagcttgaag gaaaaatgct
     4141 tgccgacatc gttaaccccg aacaacgcac cgattttcaa atatcatgga aaaaagttag
     4201 ggctggacaa accattacag aaactattca tgtgctcaac cgaaacggca acagccacta
     4261 ccttatggtt accgtatcgc ccgagttgga ccccgctgga gctataacca aagttctgct
     4321 tattggtcag gacatcaccg aaatgaagca gctggagatt aaagcccaac gccaagccga
     4381 agagattgaa caaaacctga aacagctgga gaaggaacag gagctatcgg cccagcgaca
     4441 gaaggatatg agcaccctgc tggaggcgct agaccaaaac tgcctggttt caattatcga
     4501 acccgacgga ttgataacct acatcaacaa taaaaacgtc gaggtgctgg gcgataagaa
     4561 ggaagatatc gagggaaaat tcctccatga cattgactac tcggccaagc atcgaccggc
     4621 tgagtataaa aaattctggg acaacatcct gaatgggaaa aagcagcagc gggagttttc
     4681 ccttaccgtc aagggggaag tcaaatgggt gcttgaaaac tttacccccg taatggacga
     4741 aagtgggaaa ctttacaaga taatcaacat cgggttcgat ataaccgaaa acaagaataa
     4801 ggaagcagag ctgacaaaaa taattgaaca gatgaagaac aaacccaaaa acctatgaca
     4861 taacacattc atgattaatc gagttagcca ttcgcctctg gatttcaaca ttttttaatt
     4921 cacttttaat ttggcacact ctaaatgatg aacaagttaa actggaatag agttaatttt
     4981 tcacttttga aatgacgctt gacaatgatg cagtatgaaa ttggcatagt agaaacccgt
     5041 aacgttatta aaacgcttct ggatgactat ggctacgact ttagagatta cgctctaacc
     5101 tcgtttaaac gaaggcttga gcagataatc tcaaacaacg gcctaaagga tgccgacggg
     5161 ctaattggca agctaaaggg caacccaacc ttttttgacc agttcctaaa tgaggtcaat
     5221 atcgacacca ccgaaatgtt tcgcgatcct tccttttggc gaaccctacg cgacgatatt
     5281 ctacccgaaa tcatccgcaa ctccaccacc aaaccccgaa tttggattgc ctcgtttgat
     5341 tccggcgagg agctttactc attgaccatt atcctgaagg aaatgggagc actcgataag
     5401 gttcagattt acagcaacgt tttcaccaat gccaccatcc agaaaattac cagcggccag
     5461 cttgacccta aggatattga aataaacgaa gctaactacg ttaggctgaa cggtaaaagt
     5521 cagtactcct cctactacaa agtagagggg aacaagtact ttttcgatag cacactggtt
     5581 aaagatgtga actttattaa gcaaaccccc ataatagaca aggatcccgg agcggtaaaa
     5641 cttgtccttt tccgcaatca agccttatac tacaaccagc tgctgcaaga acgggtttta
     5701 caaaaaattg cagaaagttt ggtggctggt gggttccttg ccttgggaat aaaagaaact
     5761 ttggaaaata caaacgcaaa caataaattt accgtattta atgaaaccga aagaatttac
     5821 aagcgcaaaa atgtttaatg actatgtata aggcagtaat tataggcggt tcagcaggta
     5881 gttttcaggt aattacccga atactgcatt ccctacctgc atcttttccc cttcccgtcc
     5941 ttctgtgcct acataggctg aaacacgtcc gcagcggctt tgttgaagcg ctctcgctaa
     6001 agtctggtat tcccataatt gagcccgatg ataaggaaca gataaagccc ggcaaggcat
     6061 acctggcccc ggcaaactac cacatgttca ttgaacttgg taataaaata gccctttcca
     6121 ctgaggaccc tgttaaccac tcgcgcccaa gcatcgacct ttcattttta actgccgccc
     6181 aggtgtaccg cgataaactc attggtgtta tcctatcggg ggctaaccgg gatggtgctt
     6241 acgggctgaa aaaaataaaa gatctgggcg gtctggcaat agttcaggac cccaacgagt
     6301 gccaggttag aaccatgaca gaggcctccc taaaaatgac acccgttgac catgtgtaca
     6361 acaccagcga aataatcaga tttttgcaaa ccattaaaac ttgaagaaat gaaggccttt
     6421 agctcaaaat caaaactcct tccattgctc ctgctggttt tgcttattgc cggtgtagtt
     6481 atcacctttg gattaatcca taacacactc caaaacacag gaggcagcct tcccgctata
     6541 ggttacgttc ttctattcct ccccgccatt attagcatcg gtctgtacgc catactcaac
     6601 aaccttcagg ttaaatccgc aaaactcgaa gcagaaatag cggagctaaa ggcagcattt
     6661 gcgctccttc aacaggttaa aaatcaggaa caagcgaatg ctacaaacca agaggaaact
     6721 gttgattatg ataaacttct gtcacgtctt attcctaccc tgcctactga taacctggaa
     6781 aaatttgggg aagcacttct atccaacatc gcccgaaact acgaaatagt tcaagccgta
     6841 ctctataccc gtgacggcga taccaacctt ttcaccttta aagctggcta tgcctacttt
     6901 tccgaatccg agcccgtaac ctattcggaa ggcgaaaccc tttccggaca ggtggcaaaa
     6961 aaccgccaaa tccttaacct cgatcaggtg cccgaaaact acatcacaat actctccgga
     7021 ctaggaaagg gttcgcccaa acacctgctg attgctccca ttgtaagccc cgacaacaat
     7081 tccatcggcg tttttgagtt ggccgccttt aaacctttcg acaacatgca ggtagaactt
     7141 tttgataggc ttggccgtaa acttggagag ttgctttgga gcagccgtcc ggaaacctac
     7201 cttaaacaca gcgcttggac ataaaaaata tataatccgc aaaaaataat taatatttgc
     7261 aggagcatga catcttttag attaacctca gtaagagaaa caaaaaatct aacccgaaac
     7321 aagatagcgt tagcctttct tgtaggccta ctcttttctt ttgctgcatg gatagccgat
     7381 ttctccaaat cggggctgac ttttagcctt caaaacatat tccgtcaaca tgctgccaac
     7441 ccaacgctgt ggattaccga cctaattcct ttcgcatttg cctacattac atggcttcaa
     7501 tcaacccgct tcatgcgtaa gacccttagg ctggaaaagg agttaaaaca gcgcgacgaa
     7561 gacattaaaa agaatgccga ttttgcaaaa aaaatcggag agggcaacta caacgcacca
     7621 tttgaagttg ccagcgaaga cgatattttg ggcaagtcgc tgcttatcat gcgcgacaac
     7681 ctccttacca accaaaagaa ggaatgggag cgtagctgga ttgccaaggg gaaagaagaa
     7741 atttcctaca tcctccgcct tcaccacaac attgacgagc tatcctacga tgtgctggtt
     7801 aagctcatca agtacattaa cataattcag ggagccctct acatttatga tgaggaaaaa
     7861 caactcctca tcaacatgac cacttacgcc tataaccggc gtaagtacat caatcaggaa
     7921 ttccgtatcg gacaagggct tattggggaa tgcgcctacg aaaaggattt tatttaccgc
     7981 accgaaattc ctgacgatta cattacaatt acctcgggta tcctgggcga taaaaagccc
     8041 aagagccttc ttttaattcc acttataacc gatgagaaac ttgaaggcgt tctggaattt
     8101 gcatccctcg aaaatgagat acccgaactc accatccaat tcctcaagga agttggagaa
     8161 ataattgccc gaacaatctt caaccttcgt attaatcaaa aaacagaacg gttgctccag
     8221 gaagctcagc aaatgaccca agagcttcgg gaaaacgagg aggaacttcg acaaaacgcc
     8281 gaggagatgc gtgcaacgca cgatgaactc gaaaaatcga accaacagct cgaagctaaa
     8341 ataaaagagg ttgaaaacgc ccaaaaacgg ttgcactccc tgctcgagaa tgcctccgaa
     8401 atcatttcca tctacaacag cgaaaaaaag ctgacctata taagcccatc ggttacaaaa
     8461 atactcgggt actccccaca agaaatgatg agcggtaagg acttcgaccg gctaacccgt
     8521 aagggggaaa gcgatttcaa cgaaatgttt cagcagctta tcgaaaatcc ccacatcgcc
     8581 atcaccatcc agtacacctt tatgaaaaag gatggggaga aaatttttct ggaggttacc
     8641 gggcgcaacc tgctcgacga tccagctatt ggtggcatca tcatcaactc cagggatatt
     8701 accgagcgaa aaagagctga aaaggaggag cgaatgcgta gcaagatgca gtcgctctcc
     8761 gaaaactccc ccgatatcat catgcgcatc aacacgcaag gccagttttt ctatgccaac
     8821 cccatggtgg agcagtacct gggtataagc tacagggacc tggttaacca gacccttacc
     8881 gccctggata ttagccctac cctctcagga tttatcaagg atgccattaa aaatataaag
     8941 gtaaataagg agaaggtgga ggccgaaatc acttttgcct cccaaatggg cgaaaccatt
     9001 atgcacatcg ctgcaattcc agagttcaac gagaacgagc tggaaaccat tctctttgtt
     9061 ggccacgaca tcaccgaggc caagcgcatc cagatggaga tccacgataa gaatcgtaag
     9121 attaccgaaa gcataaacta cgcacaacgc attcaaacct ccatactgcc aaacaacagg
     9181 attatcaggc agtaccttcc caagtctttc atttactacc acccccgcga tgtggtaagt
     9241 ggcgattttc catggttctt taccaaggat gactacatat acattgccgc cgtggactgc
     9301 acagggcatg gggttcccgg agctcttctc tccttcatcg gatactttac gctgaataac
     9361 gtggtggacc acgactcgtc ctacaccgcc ggcgaaattc ttgaccgact tcactatggt
     9421 gttcgtaaaa ctctgaaaca ggatcgcccc gatgctgatg cccgagatgg tatggacatc
     9481 gcattctgca aaatcaaccc taaaaccaag gagctacagt attctggagc ccatcgccca
     9541 ctctacctaa tgcgaaacgg ggagctaatt gagtacaagg gcaaccgaaa agctataggt
     9601 gggattcctc accccaaaaa ggctgaagag gatttcgtaa accacctcat ccaaattcag
     9661 ccgggcgata ggatattctt cttttccgac ggaatagttg accaggttgg tggaccagac
     9721 aagcggaagt acagctcgca gagaatcagg gagctgataa ccagcaaccc acaaaccccc
     9781 attcaggata tggccaaggt tttctctcag gattttgata catacaaggg tgatttaaag
     9841 caagttgatg atattctttt aattggaatc gaattctaac attgctattt gatagtaaat
     9901 tatataattt tacgggctat ggatactaaa aatgtcaaag ggtttcttga gtttgtttac
     9961 gacttctaca agtccatgaa agctcatgaa atcaccctgg tttacgaggg tgaaattacc
    10021 caccaaataa ccaaagcctt tacctccctc acagaatcga atatggccaa ggaggaagag
    10081 gccaactcgg tgcagaaaaa ggtattccat gtgatggtgg agtgccttca aaatatcagc
    10141 aagcatgccg acagctttgg ttcggatgac ttcctttttg caggtcgcgg catttttatg
    10201 gtcagcaagg gcgatgccga gtaccatgtt accacaggga acattattga gaacagcaaa
    10261 attgaggagt tgtcccaaat cctcgacaac atcaacaatc tggacaagga aggacttaag
    10321 gaactttaca aaacccaaat gagggaagga aggctctccg aaaaaggtgg agcagggcta
    10381 ggatttattg acattgcccg taaaacaggc cggaagctcg actaccattt cctgcccata
    10441 gatgaggaca cccatttttt cgttttaaca tcaaccattt cgagaactga gtaaatacta
    10501 aacctatgga aacaatcaaa ataatgggta ctgacgacac cccaaccgtt atactggatg
    10561 ccgaaaacga aatttttgaa atttccggtc gctcacttcc cgaagatgtt accgccttct
    10621 acgaccctgt cctatcttgg ctcgatggct atgcttcgtc ccccaaccca aaaaccatat
    10681 tcacctttaa gctggtttac ttcaacaccg ccagctccaa gcttcttctt gacgtgctga
    10741 tgaaacttga gcagatgcac gaagctggcc atgacgtgct ggttagatgg tacttcccgg
    10801 aggatgatga ggacatgcag gaagcaggcg aagaatatgc cgatattgtt gatgttcctt
    10861 tcgaacaggt tagctacaca ctgtcatagc atacttcttg catatttatg ccaatgccga
    10921 ttccgtttaa gcctgcatat cttttggggg ctgtttgcaa tttaccgatg atctaaaaga
    10981 tgagcgagga gatacttagg gccttaatgc agctctttgc aataattgca aagcaggacg
    11041 agggtgttga gatgaaggaa cgtgactacg ttgaaacgtt cctcacccag cagcttaacg
    11101 acgaagcggt agcagaatac cttaggcttt tcgacgagca cgcaggcctt ttagacaagg
    11161 agatagggtc cgacgacgag gaaaggtcaa aacctaaact cacatcggta aaggattccg
    11221 ttcgcatcct cggcatctgc aagaaaatta acaaaactct tacccaaaaa caaaaaatag
    11281 ttgttctggt tcgcctctac gagcttatca atgccgaccg taagtttacc gaacaacgca
    11341 tggccattat taacaccgtt gctgaagttt tcaaactttc gcacgaagag attaacgaca
    11401 tagaaaactt tgtagtaagg aataatcccg aggagttaga ccgacccagc atactaacca
    11461 ttcacgacag accttttaag ggcgaacagt gcaagcatat ccccaccgaa gctctcgacg
    11521 gatttattta tatccttcaa atcaaaagcg aggaccttta ctttttacgc tataccggga
    11581 atgaggatat ttacctgaac gggctaccca tcagcaaccg tcgtatctac ctgtttgcaa
    11641 gcggtagctc cctgaagctg cccaagggca agcctgtgta ctacaccgac gtggtggccc
    11701 acttcctttc tgactccact actatcagaa tatcctacag ggtggacaat gcaagctaca
    11761 ccttcaagaa cggaaccgtt ggcctgcaca acatcagctt cagcgagggt catggtaagc
    11821 tgataggaat tatgggcgcc agtggtgccg ggaaaaccac tctgcttaac gtgctggcag
    11881 gcatcgaaaa gcctacttca ggccatgttt acatcaacgg ctacgaccta cacgccagta
    11941 gcaatatgct cgaaggtgtt attggcgtta tcccgcagga cgacctgcta attgaggagc
    12001 ttacagtttt tgaaaatcta tactacaacg caaaactttg ctttaaggat aagaccgaac
    12061 ctgagcttat cgccatggtt gataaaaccc tatcgaacct aggcttgctc gagcaaaaga
    12121 atttaaaggt tggcaccccc ctgaacaaga tgatttcggg agggcagcga aagcggctta
    12181 acattgccct ggagctaatc cgcgaaccct caatcatgtt tgtggatgaa cccacctcgg
    12241 ggctttcatc gcgcgactcg gaaaatgtta tggacctgct gagggaactt acccttagag
    12301 gaaaacttat tttcgtggtc atccaccaac cctcttcgga catttacaaa atgttcgaca
    12361 acatgatcat cctcgatacc gggggctaca tgatctacta cggcaatccg gttgaagctg
    12421 tgatgtattt caaacgcctc gacatgcaaa taaacagcga ggttggagaa tgcccggttt
    12481 gcggtaatgt taaccccgag ctgctattca acattataga ggcaagagtg gttgatgagt
    12541 ttggccgcta cacccccata cgaaaggttt caccccagaa atgggaggaa catttcgcca
    12601 gcaacatcaa gcctccggtt attcccgacg aaaaggtcga accaccacgc accctcaaca
    12661 tcccctcgcg ctttaagcag ttcaccatct acaccatgcg cgacctgcgt tcaaaaatta
    12721 gcaatagcca gtacatcatc cttaacctgc ttgagacccc aattcttgcc ttcatcctgg
    12781 cctacgtggt gcgctacatt gccgatccca actctaagat ttacatcttc agggaaaatg
    12841 agaatattcc catctacatc ttcatggctc ttattgtagc gttgttcatt ggacttaccg
    12901 ttagcgccga agaaattttc cgcgaccgaa aaatactcaa gcgagaggca ttcctcaacc
    12961 ttagccggtc gagctacctg ctctccaagg ttgccatttt attcctgcta tcagccattc
    13021 aatccgctac ctttatccta atcgccaaca gcattctcga aatcagggac atgtacttcg
    13081 attactggct ggtgctattc accacggctg cagcagctaa cctgctaggg ttgaacattt
    13141 cggccacctt caactctgct gtaactattt acatagtaat acccctggtg atgatcccca
    13201 tgatggtgct ttcaggcgcc atgttcagct tcgataagct caaccggtct attggcagct
    13261 tcaacagggt tcccgtcatc gccgagctta tgattaccaa atggggatac gaagccctta
    13321 ttgttcacca gttcaaggac aacgagtttg aaaagaactt ctacgaaata gaaaaaaacc
    13381 ttagcaatgc aaacttcaag gtaacctact acatacctga gctagagaaa agagcaaccg
    13441 atgccttgga cctgcttcag caggaaaaaa gcaaagccaa ccaggaggcc ctaaaggaca
    13501 agctggccgt tattcgtaaa gccgtgataa ccgaaacaac cgttacggct ccttcggtac
    13561 cctttgccta cacccaaaag cttacacccg agcaatttga cgaggaaacc gcatacgccc
    13621 taatcgacta ccttgatgcg ctccacaggc tttacagcga catctaccaa acccaaaatc
    13681 agaaacgcga caggataatc aactaccttg taaataccaa cgaaaagcta taccaggcaa
    13741 agcgcatggc ctaccacaac gagagcatct cggatttagc cacaaaggtt ttcgagaaaa
    13801 ataagattct ccagtacggc gatgagctgg tgcagcagta tgatccggtt tttcgagatc
    13861 ccctccccag acatattctt gacatccgat ctcacttcct tgccccacga aaacactttc
    13921 ttggtatgta ttttgatacc ttttggttca acataatagt aaactggata atgtcaataa
    13981 tcctgtacat taccctctac tacgaatcgc ttaaaaagct gattgaatta ttttcaaaaa
    14041 ttaaggtgcg aggctttttt aaagcaaaaa tttcataact ttactttgaa atttacgaca
    14101 tttcccctta aacaggaacg caacatgaaa agcaggtttc caattgtaat agttgccatt
    14161 tgttccctta gcattgccct atcctcttgc aaatcgtgca acaaagataa agacaacctg
    14221 ggagaggagc tggtaatccc ggattcgcta aaatgggata cacctttacc catgggcaag
    14281 cctatagttg acgagctgat gcagaacata tcgtcgccgg ttgaaacagc tgccttgatt
    14341 aaaggtctga aaattccttt caacaaggac tatatggcca gcacaaagca ggaggagtcg
    14401 tttaatacga gtttcgacaa agctttagcc ctgggcattt atggttgcga tctcggttat
    14461 ctcaacatgt acgagaaaac atcgtttgtg gttgactaca tctcggtgat caaacgcttt
    14521 gccgacgaca tacaggtggg tcaattcttc gattttgaaa cacttaaaag actggcaacc
    14581 aataaagaaa acctggactc cctcatcctg atctcccagc aaagttttaa ccggattgat
    14641 agctaccttc gcgaaaccaa ccgttcaacg ctaagtgcgg ttattgttgc gggggtatgg
    14701 gtcgaaggcg cctaccttac ttcccgtgtt gcgcaaacca gtaaccatcc ccgcatacgc
    14761 gaaaccattg gcgagcaaaa aaccgttttg gggtttcttc tggcactgct taaggcttac
    14821 agcaacgacc ccaacatggc aaaactcatt gctcaactcg aaccacttaa gaaccagttt
    14881 gataacgtta aaattaccta tgagctggga gaacccgaga tgattgaagt taacggggag
    14941 cttaccttta ttcagaagga caagcagctg gttgatatgg atgatgtaac ttataaggag
    15001 attatttcag gtattcaaac ccttagaaat tcgctcatta aattgtagga aattttttgg
    15061 ccatgaaaag gatattacta gcggttgcaa ttatttgggt gggtctagca tttacagcaa
    15121 gaaacgctaa tgctcaatcc gaagaggagt tggttcaaat atgcggaatg attgcaggag
    15181 aagcacaata ccttaaagac ttcagggtta agctggatgc aggcgatccg ccacctgttc
    15241 agcgaattcc cattctttta aagaaagatg tgaaataccg tttcacagtt tgcaactcaa
    15301 aggactatga gggaaaagtt gtgctacagc tttttgacaa caatcgtatg cttgcaacca
    15361 cctacgtaat aggcaccgga aaggattatc catccattga ttgggtgtgc acaaaaaccg
    15421 gagcctacca ccttttcttt agcttcaggg acggtaaggc cggactggca gtaggcctgc
    15481 tctcacttgt cgagatgatg taaatctata aaagaccgta cacccatagc taaaaaagaa
    15541 ctatttatta aagttccttt tttagcgcgt ttgagcatat ccatttttgt ttagctaatt
    15601 acgtctccgc ttctgcaacc attcttctta tggtagtcca acgttccccg tccattttgg
    15661 ggccaagcac ctctatgaca tgccccgaca gaatagcgcc aatttgcccg catttatcaa
    15721 gcggtaatcc tttcatcagc ccgtaaagaa aaccggatgc atataaatcg cccgcaccgg
    15781 ttgtatctat actcgaaaca tcaataatgc ccacttcgta gaagtcggaa tttcgcttta
    15841 tgtacgaccc tgaagggccc agcttaacca cggctatggg cacaatttcc gccaagactt
    15901 caactgcctc cctgggcttc ttgcctgtaa aggccttggc ttcatcctca ttggcaaaaa
    15961 gaatggttac gtactttttt actatctcgt tgagaaaatc acggttctcc tcaacaacgt
    16021 tatagctggc caaatcgaac gaaatctgaa gacctttggc agaagcaatc tccaaagcac
    16081 gtagtagcat ttcgtggttt tgaaccaggt agccttcaac atgaaagtag tcaaaacctt
    16141 cgaacatttc gggttttaaa tcaactgcgg tcagctccag ggcagctcca aggtacaccg
    16201 caaaagtacg ctccgaatca gtgctgacca aagccagcgt tctccctgat tcggctttgc
    16261 cctgtagcat gataggattt atcccgacct cctccatgct tgttttaaat gctctcccaa
    16321 aaacatcgtc tccaattttg ccaataaacg aggttgccac atccatgttg gcaagtccat
    16381 gaatggtgtt tgctgctccg ccgcccgctg agagcgattg gggcatgtcc ctggtaaaac
    16441 taattgcctt tcgcatgaaa ccctcatcaa ccagctgcat gctgcctttt ggaagcttca
    16501 acgtctctag caatccatcg tttggtagct gggtcattat atccaccagc gcattcccta
    16561 acccaagtac tttcggcata ttatacgtta tgagaggtat tgtctcttaa gcagcatacc
    16621 tgctttaccc ttgcaaagcc taccaaagat agctattggt tcgtcaagaa taccaacgaa
    16681 tagttaataa ttttcaaaat ctcttaccct taaaaatgca actcgttgcc ttataaatac
    16741 ggttgttcac gcttcagctg caagcgttag aaatattgcc ggttttaaaa gaaaaaaccc
    16801 cgataagcat cggggctggc tcctcatcta ggacttgaac ctaggacccc ctgattaaca
    16861 gtcaggtgct ctaaccaact gagctaatga ggaatgcttt aatttcagcg gtgcaaatgt
    16921 actacaaaat attaattcgt gccaaaatta aatggaattt ttttcactgc actcatatcg
    16981 aaattgctga aatcatggca aagtaacacg ggttaccatg gcctgttgaa ggacacttat
    17041 tcccaaatag cttccggtaa aatgggtgat atagaacgta gggattacct ataatcgttg
    17101 gtgtagaacc tccatggccg gctggaccag tagctaccag catagttaac cccaatacgc
    17161 ggtgcggtta aaatggaagg gaaaaggcca gagtcctcaa tccagattct ttgagagctg
    17221 cctaagttct cagcatagaa gctcctgtca attttcaaca acctgctaac tcgaccgggt
    17281 ccactacaac ctgctactcc cctaatcaat accgcctcgg gttcattctc aaccgatgcc
    17341 accacgttga gcatccagtg cataccatag acaaaataga tgtagagtat cccgccttgt
    17401 tggaacatgg catcgttacg gggagtgcgc cccttgctgg catgacaggc caaatcattc
    17461 atccccttat aggcctccac ttctgtgact atatgacttg aactcgttcc atcctcataa
    17521 attcggcaga tacgcttacc aaccagctca ggggcgacca gtagcacatc gcgtaggtaa
    17581 aagtcgggtg ttagtctcgt cgcgtccatt tacccatcac tttaaacact tttccctccg
    17641 gcaataacca gagtatctac agcatcttgt ttaatctatt gctgatgata cttagtatct
    17701 tttctgatga gcctacaccc gattttacat aggcaccagc actttcgcct gcttggctaa
    17761 gcaatgtcgg gtcatttaga agcctgtcaa gcaaatcgtt gagttcagcc tgcgatttga
    17821 tagaaaaacc cgctcctaaa gcaattaaat ccattgcttc ttgaaatttc aggtaattgg
    17881 ggccaaatac tactggtatg ccaaaaactg ctgcctccag ggtgttgtga ataccagcgc
    17941 caaagccacc gccaatatat gccaggcagc cataccggta ggttgaagag aggaagccta
    18001 ttgtatcgag taccaacagt cgtgcctgct ttggcgaagt tccttcgggc cgggtaaaac
    18061 aaatggcctc aacctttgaa gcgctcacta ttgaacccac tctgctcggg ctaatttcat
    18121 gtggagcaat gatcatctta actctatcgg catttgcgga tagatagttc aagagtagct
    18181 cttcatcctt gggccaggta ctcccggcca caattacctt ttcaccatcg caaaaggtat
    18241 cgagaagggg aatcgtagct gactcagagg caactctcga caccctgtcg aaacgggtat
    18301 caccggtaac agttacctcc ttaataccca cggagttgag cagctctagc gaaaggtggt
    18361 tttgaacaaa aatatgggta aaatgcgata aaaatttacg gtaccacctc ccgtaaggct
    18421 tgaaaaaaat ttgggtttcc ctaaagttgg ccgaaataag gtatgttgga atttccctgg
    18481 catgaagctc ggagagaaag tggtaccaaa actcatactt aacaaaaatg gccatttttg
    18541 gttttacagc ctcgacaaat cttttggcgt tcctttttgt atcgagagga aggtaaaaaa
    18601 tgtagtcggc tccgctataa tttttcctca ctttatagcc cgatggagaa aaaaaggtaa
    18661 gcacaacttt tagcgtagga taactctccc ttatgcgttc tattaatggg cgtccttgct
    18721 caaactcacc caacgatgag cagtgaaccc aaatttttgg gtcattattt tgggccagca
    18781 attcaacttc atcgagaagc ccccttcgtc cggccagcca actttttgcc tttgggttta
    18841 ctggtgaaac caaccatata agtagcaggt agatacggat tcccaagtta taaataagca
    18901 ccatagaaca gcaaacaatt aaagaggggc aaaaagggta agtaaagata aaacgcttcg
    18961 aaatatagcc tatcctcaat ctaacagtat aaaaaccccg gtagtagagg aaaaatcatt
    19021 tagtcaattc cttcgttagg gtccggaccg gtgtactccc taagaacgcc tcgtttagaa
    19081 ttctttatga agttaactat tagatctctt tcggccgttt gcgggatatt gtcaataatg
    19141 aaggcaagtg cctgcgaaac atttaaccct ttattgtaga ttatacggta aatctcctga
    19201 atatcggcga tttgctgatg ggtaaaactt ctgcgacgca acccaaccag gttgataccg
    19261 gcaaaggtta taggctcccg tgcagcaatt atgtatgggg ggatatcctt gttaaccttc
    19321 gaaccgccct ggatgataac atgggcacca attttttcga actgatgaac caatgaatgg
    19381 gcgctcacta tggcaaaatc gtcaatctca acctctccgc ccagctgtgt tccattcccg
    19441 aggattatgt tgtttttcag tatgcagtca tgcgctacat gtgaatatgc cataagcagg
    19501 cagctgctcc caacaatggt ttttcccttt gaagcagttc ccctgttaat ggtaacgcat
    19561 tcgcgcatgg tggtgttatc gccaatctcg gcagttgtta cctcgccttt aaactttaaa
    19621 tcctgaggga tggctgaaat taccgctcca ggaaaaattc ggcagttttt gcctattcgg
    19681 gcgccatcca ttatggtaac atttggacca atccaggttc cctcacctat aataacatct
    19741 cccgctatgt agctaaacgg gctaatggta acatccttac caattttagc gtcgggatga
    19801 atgtatgcat taagttgtgc cattaatttg gatttttaat aatttgagcc attagcacac
    19861 cctccataac cagctggtta ccgacgaatg cctgaccggt catgttggca atccctctac
    19921 ggatgggttc attcaaggtt aagcgaaaaa ttatagtatc accaggaaca accttgtgtt
    19981 taaaacgaac ctggtcaatt tttaagaagt aggtggtgta gttttcaggg tcgggaacag
    20041 tgttaagaac cagaatgcca cccacctgag ccattgcttc aatctgaaga actccgggca
    20101 taacgggttc gtggggaaag tgtccggtga aaaaagcctc gttcatggta acattcttca
    20161 aacccacaac ggaggttcca ttcatcgaaa ttattttatc aaccagcagg aatggcggac
    20221 gatgtggcag tgttttttta atttgattaa tatccattag gggaggaaca ttggggttgt
    20281 actcgggtgc agagggcttg agcagctcct ttttgtactg ctttctcagt attttggcaa
    20341 attcggtatt ggcccagtgt ccggtgcgaa tggcaattat ttttcccttg atgcgaacac
    20401 ccaccagcga caagtcgccc accatgtcga gaagcttgtg gcgggcaggt tcgttgtcga
    20461 aatgtaggtc aaggttgttg agtattccct cgggtttaac ctttacgtgt ggtttgttga
    20521 agaggtcggc aattctgtcg agctcgggct gcggaacctc atgctctata atgacaatgg
    20581 cattttccag gtcaccaccc ttaataaggt tatgctttag caacacctcg agctcgtgaa
    20641 agaatacgaa ggttctgcaa ggtgctattt ggctggcaaa ctcgtcaata ttccccattt
    20701 tggcgtactg atgacccagc acccttgagt tgtagtcaac catcaggtcg atgctgaatc
    20761 cgtcgtcggg ataggcaact atttcgatac catttttttc atcggagaaa accattttct
    20821 cctttatggt aaagtagtta cgaatggcac cctgttcaac tgttccaaca ctagcaatag
    20881 cctcaacaaa gaacctggag ctaccatcca taattggcat ttcggggcca ttcactttta
    20941 ccagggcatt atctattccc atgccgtaaa gagcggcaac catgtgctct atggtgttga
    21001 cttttacatt tttggtgcct atggatgttc ccctggaggt gtcaataacg ttatcggcaa
    21061 ccgcttcaat cttgggttgt ccttccaaat cggttcgttg aaagttaaca ccggtgttca
    21121 cctctgccgg acaaatagta acctcaacat caacaccggt atgcaacccc tttccggtaa
    21181 gggtaactgg ctggcttatt gtgcgttgtt tatctgccat aaaaaggttt tcaaattaaa
    21241 catattccct ggttccaggg gttctacata ttcaaggttt ctttttaata agtcaaaaaa
    21301 tataaagaga aaccggctat agcaataatt ataataattt tttcttcaac tcctcaatgg
    21361 tgcgctgcat ttcgtttagc ttattggcca tgtcaggcaa attcttataa acagccatag
    21421 accggcgttg tttggagaca tcaaatgccg ggcttcccaa aataattgta ccgggtttcc
    21481 gaatgtcgcc ggcaataccg gactgtgcgc cggccatcac accatcggct attttgatgt
    21541 gggggctggc tcctacctgg ccaccaaaca tgcagttttt cccaatttta gtcgatccgg
    21601 ccactccgca ttgggctgcc ataacagtat tttcaccaac ctccacgttg tgggcaatat
    21661 gaacatggtc gtcaatttta acgcccctat ggagaatggt agaacccatt gttgcccggt
    21721 caacagtagc atttgcacca atttctacat aatcctcaag gactacgttg ccaatttgcg
    21781 gtattttttt gtagttagca tcctcctggg aggcaaatcc aaaaccgtcg gcaccaatta
    21841 ccgctcctgc atggataata caattgctcc caattgtgca ttcatggtaa atcaccgcac
    21901 cagggtaaat ggttgtatta tcaccaatta taaccccctt accaacaaat acctgagggt
    21961 aaattttaac cctgctcccg attctgacat cgccagaaat gtaggcaaac gcacccaggt
    22021 aaaaatcatc cccataggct gcagaagggt gaatgaatga aggtgtttca acaccgctaa
    22081 gctgtggttc tgtttgctga gcatagagtt gaagcaagga ggcaaatgcc tcataggcat
    22141 tcttaaccct caccagggtg gctttaaccg gttgctcggc tacaaagtca gcattaacca
    22201 gaacaatgga agcattggta gtgtaaatat atggagtata ttttggatta gccaaaaaag
    22261 ctagtgaacc aggagatccc tcctcaattt ttgaaaccgt gctaaccttt acctcggggt
    22321 tcccttccac tacccctccg aggaagttgg ctatgacttg cgctgtaaat tccatgttaa
    22381 gaatgtatta attgaacgca aagctagcta tttatcttaa aatttccctg agttctttag
    22441 ggaaacaggt gaaatatttt ttggtttcgt ttgataaagc gttggatttc aggatgtcag
    22501 aagcctcagc gatgtcaacc agctgcccat tgctatgtag tatttgaatg gattcctcac
    22561 ctggctgata ggcactaata ccgacagagt cggaaaaaac aaaataatcg gtatattcag
    22621 taaagttggg gaaagcggag gagaacaaac cctttaattc agccaaacgg ctgctacttg
    22681 aaggaatagt tgccagctca atgctgagta accttcggtt gataatcatt gaggaaagtt
    22741 tggccagcac cgggtcggag cagttcatcc agtcttttgc tgcaaccatt atatctgaat
    22801 catccagcat agcaaagtag gaaagcgcct ttgattgggg cgtaaaaaaa tcatcgccgg
    22861 taacatcgtg agcaaagaag tacagcagag gggctgatat gttcatcggg aacccgcttc
    22921 taaccagctg tttagctctt tgtaggagtt taaccagtgt ttgttcggca gcaacaaccg
    22981 ttttatgcag gtaaacctgc cagtacatca gcctacgggc aattagaaaa ttttcgatgg
    23041 agtaaatgcc ttttgcttca actaccaggt tgtcgtccac cacgttgagc atttttataa
    23101 ttcgctccgt gcttatggaa ccctcaacaa ccccggaaaa aaagctgtcg cgtttcaggt
    23161 agtccatcct gtccatgtcg agctgtcccg aaataagctg gtgaaggaat ttccggtgat
    23221 accgatcctc aaaaatttcc agggctagag aaaggcgtcc gttaaactcc tcattaaggc
    23281 tacggataaa agctagactt atagattcat ggctgacacc gggcacaatt gtgttttcca
    23341 gggtgtgtga aaatgggcca tgtccaatat cgtgaagcaa aatggcagca catgcagcct
    23401 cggcctcttc ctcagaaatc acaaacccct tgctgcgaag agtatctata gccgtatcga
    23461 ggaggtgcat cgcaccaagg gcatgctgta aacggctatg atttgccccg ggataaacca
    23521 ggtaggttag gccaagctgc tttattcctc taagcctttg aaagtaaggg tgctcaatga
    23581 tatcaaatat cagcgggtag ttaatgctga taaaaccatg aaccgggtcg tttacaattt
    23641 tgttcttgtt gattttgtca tttctcatat atggtgaagc aaaacttgtt gtcgttatca
    23701 aaaaaagcca gtaacattgt gtgaagacaa atttgccgag taattagagg gctcgattgt
    23761 agcaatactc agtgttttct acatgaattt tcattcaacc tgcttgccag acataacttc
    23821 catttatttt accctacccg ggttttggcg cacaaaatcg ctccagcttg atgcttttcg
    23881 actagcaccg actgtcgcac cctgaaagaa gtggcatacg gcagcggcca aaccatcggt
    23941 agcatcgagg tactcctgcg agaaatcctt ttccccaaga atggatttca gcatggccgc
    24001 cacctgctcc ttcgaagcgc ttcccgagcc ggttatagcc tgctttattt ttcgcggtgc
    24061 gtactcaaaa attggaattg agcgagaaag aacagctgcc atggcaaccc cctgcgcccg
    24121 gccaagcttt agcatactct gaacattctt accgtaaaaa ggggcttcaa tggccacctc
    24181 gtcgggcagg tactggtcgg ccagctgctg tacccggtcg taaatggctt tcaacttcag
    24241 gtagtgatcc tcgtacttct taagctcaat aactccaaga acaaccagct ttaggtcgtt
    24301 tttcccgcta cacctcacaa ttccgtaccc aagaatattt gtgcccggat cgattccaag
    24361 aattatcttc tctgctggtg tggagttggt catgaattta ctttactcat aacaaacatt
    24421 tataccaaaa tggagacctc gtaaacctgt taaactggtt cgaaagcctg gcttatagcc
    24481 tgtagttttg tttagcacac cctaccatgc gccaatttcc ccttgtagtc ctttgcctac
    24541 aaatggttga acgccaacag cggctttacg tgtataacct tttgcagtta ctccaaggtg
    24601 ggtaaaagcg gcattcaggc tttcttcgtc catactacca aacggatggg taatatcatc
    24661 ggcagcggaa acatctacag gaatcccatc aaaataatcg ccctcgttat ttgagttccg
    24721 gatagaaaag catataggca caataaccca gtcaatgctg gggtctctaa actcaaacga
    24781 gtacatccca acaggtttgc catgcgtttt tgaaccaacc agcgatactt caatatgagg
    24841 cttaagcccg ttaatcacga gctcgctggc cgaagccgtt ccgcttgtag tgatgaaaac
    24901 aacccggtta aggggaatcg aattgtcctc gtaggtgaaa gttatggagg agttatcgcc
    24961 ttggtgcttg tcgttatggt agtaggttcc gaaaactttt ccaaaggcgc tgctgccggc
    25021 aataagactt gccaggtatt tggcaacacc tacctctcca ccgccgttgt acctcaagtc
    25081 aactaccagc tcgttaatgc cctcctcctt gaattttgag aaaagaccgg ttagctccgc
    25141 ttcggttggg ccaataaagc tttctaaaac aaaatagcct accctgaagg tgccggtggt
    25201 ataaatcgaa tccatcaaaa cggagttcat ttcaatctgg ctcttggtaa aaatctgctc
    25261 catggtgcta ccttcaggag atttaaataa gaatttcttc gatacaccta cctcggatgg
    25321 gcctatcagg ctagaaaagt tttcggaagt tggggtttgt ccatcaatag aaataatctg
    25381 ccaacccctg tcaattccca tagcggcaag cggggagttt ttaaaaacaa aggtgatgta
    25441 aagttcgtta ttcgcactaa accccgagcc aaaaccaaac cccacgtacg caccctggtt
    25501 gtagtatgca tcaaactcgg ctttagttgt aacatagctc cagcgatcgg gcggatatac
    25561 tcgtaaggca ttcactagct ccacagggtc tttgtaggca tttggatcaa cctctggcat
    25621 ctggtcgtac caatagtaca tgtcgtccat cagattataa acctcctggt tgacgttcac
    25681 cttctgctca tcttcgttat cacacgatga taaaaccatg gctgctataa ctgccagcga
    25741 taaaatttta tgccaattca tgggttattc tatttattag ggattgcaca tcattatcat
    25801 acagcacata aaacgtttct gaattaatta tgattaacct catgctaata ttacttaagg
    25861 tttttgccct tcattatcga gttttctgcc taaatactgc tggaagagaa ttgctccaat
    25921 aatacaaatt aatcccgcaa cagtggtcca aattatcttc tcgtttagta ttaagcttat
    25981 aaaaatcaga gcaacaaaag gcacaagata tgaaaagttt cccaactttg ccgtgctggt
    26041 agcaaggttt agcgctttta accaaagcac aaatgttatg cccatttcaa aagctcctat
    26101 gtaggcagag gctaaaattc cctgccaggg cagaggtttg gcaaagccac caagagtaac
    26161 catagcaata aggcagaaaa ttgaagcaga tgcaaaactt aaaaatagtg ttacaacctc
    26221 gtcgccagcg tttttttgca tattaacaat ccaaaaagta gcccatacaa tagagctgga
    26281 tagcgccaaa accacaccaa gagggttgct gcgtgaaaaa atatttatgc taccctgtga
    26341 tgaaatcagc gtaactccgg taaagcttac cagcagggcc agcaggtcgt accagtgcag
    26401 cttttgtccc aaaaaaattg cagccagcag caccagcact atgggccaaa tgtagttcag
    26461 gggttgggct acctgggccg gcaaaaggct gtaggcttta aaaagaacaa ggtagtagag
    26521 aaacgggtta agaaatccca gcattacaga ccttccaagt tgagctggtg tagctgctga
    26581 cagctgctta aacttatgct gggaacacag aaccaggaag agaatggtag ttgccgctaa
    26641 ggatgaccag aatagaagct gcacagtgga aagataactt agccctattt tgaaggctgt
    26701 agcagacgtt ccccagaaca aaaccaccaa cgaggcataa acgtaggcgc gattttggct
    26761 tttcagtgtc atccgaaggt agttcaaata attgggcaag ttagaaataa tcgcttagcc
    26821 ggagaaggaa atgccgaaat actacccctc ttaaccaaaa aataaagaat tcgccatgca
    26881 gcaagtttgg ctttactatc gtatttaata gttgaaatta aaagcaaaat gaaccatcct
    26941 taaaaatggc agctagcata tacacattgc tagtggcgga tggtttggta acttatcaaa
    27001 acaactaaaa atatgcacat gaaacagtta gcaattttac tggttactct tgttttccca
    27061 ttagtgctgg aagcacaggg gattgccgaa gaaatgtttg agcgctatgc cggaaagccc
    27121 gggtacacct ccgtcgccat tggttcggaa tttttaaact ttgtggcagc cctagacagg
    27181 gaggataatg agctgcagga tttatcgaat aaaatcagca gcctgaaaat tttagtggcc
    27241 aagcagcagg ttgaagaatt cggcaaaacg ctggaaaggg gaattacccg cgaagggtat
    27301 aaggaggtta tgcaagccaa cgatgagggt gggttggtaa cattctacgc actcccaaaa
    27361 ggagacaagt tttccaactt catactccac gtaaccaatt cggatgagga ggtaatgctg
    27421 gccattgcgg gaaactttac gctatcggat ctgtcgaagc ttggtggctc ctcagaagat
    27481 gatgatagct acatttccct gttaagaaag ctggagaacg aaaagtaagc tcaaaacgaa
    27541 catccttcga caaggttgat gtgtagctga tttcttctaa ttttacagaa atcccagctc
    27601 caacatggcc tcgtcgctca tccggctttt atcccagggc ggatcaaagg taaggttgac
    27661 cacaaccccc gaaaccccct cgatagcaga tactttctca tgcacctcat caaccacatc
    27721 atcggcaagc gggcagttgg gtgcagtaag ggtcatggta atggttactt tagaatttga
    27781 gtcaacatca acttcgtaga ttagccctaa gtcataaata tttacaggga tttcggggtc
    27841 aaaaacacct ttaaggactt ttactatatc ggattgtata gccagctcgt ttctcgattc
    27901 catagtttca caatttatag ggtagaaaca aaattaacag gctaaggtta agcttttcag
    27961 gtagcataaa taccgctcgg ccgcctgcta gccaaatatc tccttaactc tttgcacggc
    28021 ttccaccagc ctgtcaacct catcataagt gttgtaaaac gcaagtgaag cccttactgt
    28081 accgtcaatg ccgtagtgat caattaccgg ctgggtgcag tggttgccag ttctcacagc
    28141 aacacccaac ttgtcaagta tcattccggt gtcgtaaggg tgaacattat caaccaggaa
    28201 cgagattatt gccgctttgt cgggtgccgt tccgaaaata cgaatccccc ctatttcgga
    28261 aagtttgcgg gttgcatact gtatcaaatc tgactcgtag gcagttgccg ctgaaacacc
    28321 tattttagtg tagtagtcga gcgctgcggc aaggcctgag gctccaatat agttcgctgt
    28381 tcccgcctca aatttaaagg gcaactcgtt gaaagtggtt ttggcaaacg taacgcggtt
    28441 aatcatatcg ccgccaccct gccaggggac catcttttcg agccaatttt ccttgccata
    28501 caaaacacct atacccgttg gcccataaat tttatgcccc gaaaaggcat aaaaatcggc
    28561 atctatgtcc tgcacgtcaa tatttccatg cttcaccccc tgtgcaccat caaccaaaac
    28621 aggaatacca tggttgtgcg ctattcgtac gatttccttt acagggttaa cagttccaag
    28681 ggtgttggag acctgagtaa ctgcaataat tttggttctg ggtgtcagta acccttcgag
    28741 ctggtctatg cagagctgcc cgtcgtcccg gaatggcaaa attttaagtg aagccccttt
    28801 acgctcacag agcagctgcc atggaacgat gttggagtga tgttccatct cgcttaccac
    28861 tacctcatcg ccactatgca caaaaacctc tccaaacgag taggctgcca ggtttatgga
    28921 ggcagtggtt cccgaggtaa atacaacttc tgacgtttta cgggcattaa tgaagtttct
    28981 tacaacctca cgggcatttt cgtactcctg ggttgactcc tcgctcagct tatgcacccc
    29041 acggtgaatg ttgccgttaa tttggttgta gaagtatgcg actctatcca aaacagcttt
    29101 aggtttctgc gtggttgccg cattgtcgag gtaaactagg ggcatgccgt aaacctcacg
    29161 ggaaagtatt ggaaaatcct gtcgaatttt ttcgacgttc agggattgag tcaggccatc
    29221 ggctgattgt tgtttaactg tatccattct atctagttta aaaatctggt tcaacctgcc
    29281 caattgatgc atgcaccagg ttaaaccacc aaatgaatgt actataagat cgccaaagca
    29341 tacgcaagct agcagcagtg cattggacag gtgtggcatc gcgataactc gccgtgaagg
    29401 cgcttatcca ccagctcgtc gagtctgtct ctaagagtag ggattgatat ttgccctaca
    29461 atttcatggg caaatccaaa catgagcatc aactttgcat cccgctcacc aatgcctctt
    29521 gagcgcatgt agaatagtgc gtcagcatca atttgaccaa ccgttgcgcc gtgtgagcac
    29581 ttcacatcat cagcatatat ttcaagttgt ggcttgctgt ttatccgtgc atcagctgta
    29641 agcagtaagt tattgttcga ttgatatgcc tgtgttttct gggcattggg tctaaccaga
    29701 attttcccgt tgaaagcccc cgttgcctgt tcgtccaaaa tgcccttgta atgttcaaag
    29761 ctgctacagt tgggcactgc atggtcaata aaggtaaagt tatcggtatg ctgcgtgcgg
    29821 tcggttaggt aaagtccgaa agcctgcgcc gaagcgccct ctcctttgag ggctatatga
    29881 aggttgttac gaataaaacc accgtgtagg gttagggtat tggtctttac acttgaacct
    29941 tcttcctggt tgataaaggt gtgggaaagc tggctgctat cgttgtgttc attctgcatc
    30001 cttacatact caagtgtgga gttggctccc gtaaagattt cggttaccac gttggaaagg
    30061 tagcgcttgg aagatagcgt atggtcgcac accagaatgt tggcatggct cgattcttca
    30121 agtataatta agttacgata gttaaccatg aggttttcct ccgacatcag caggttaatt
    30181 atctgaatgg gtttttcttc tacagcacct tttggaacgt aaataaaaat gccatcctga
    30241 gcaaaagcgg tgttaagagc aaccaggcca tcggcgttgc actgtgcata aacgttgtag
    30301 tgtttggcaa caatttcagg aaaatccttt gcagcctggg ctaaacttcc tacaatgatg
    30361 ccattatcta acttttgaag ttggccgcta cttggcaggt aaaatccgtt gagcaccatg
    30421 gctaaatggg tgtccaactc aggaatgtcg cacctgaaga tatcatccac tctaaacgaa
    30481 atcttcttag gagtaaagta cttctcgtac tcatgctgaa agaggggttc aacccgggag
    30541 tacttgtagt cttcattttt tgcagatggc agcccaataa tcctgaagtt ctggattgcc
    30601 tcttcacggt gtttgttcaa ggtatccgag ctaccgccag taataaggtc atgattatcg
    30661 aggtagaggc taaccaggtc atccaaaagt ccagctgtat cggttttact catggtttca
    30721 actttttact taacccttca ggttgccatt cttaatccaa tcgtaccctt tttcctcaag
    30781 ttcaagagcc agctccttac cggcactttt aacaatttgg ccgttgtaga gtatgtggac
    30841 aaaatcaggt acaatatagt cgagcaagcg ctggtaatgg gtaattacaa ttacggcatt
    30901 atccgggctt ttgagcttgt taaccccatt ggctactatt cgcagggcgt cgatatccaa
    30961 acccgagtcg gtttcatcaa gtatagccag cttgggttcc agcatggcca tttggaatat
    31021 ttcgtttttt ttcttttctc caccggaaaa accctcgttt accgaacggt tggttagggc
    31081 agagtcaatt tcaaccagct ccttccgctc acgcatcagc ttaagaaaat cggttgccga
    31141 aaggggttca agacctctat acttgcgctg ctcgttaact gcagccttca taaagtttac
    31201 catgctaacg ccgggtattt caaccgggta ctgaaagctg agaaaaatcc cctcgcgcga
    31261 acgcacttcg ggtggcatct ccaaaaggtt ttttccatta aaggtaacct gcccatgggt
    31321 aacctcaacc atctccctgc cggttagcac cgaagctaaa gtgcttttgc ctgagccatt
    31381 cggacccata atggcgtgaa cttctccggc tttaacctcc aggtttatac cctttaatat
    31441 ttccttatcg tttattctag catgtaaatc gttgactatt agcatactat gatgattaat
    31501 gtatatgttt ccaaattaat atttccgtgt tgacccacca taaaattaca catcaaaatt
    31561 catgtaaacc ggtatatctg aggccttcaa ttcctaacga gtaggttata cgggcgatta
    31621 agccggtagt attcaagtag ctttaccatt tcacctttct tcgaatgttt ctaacctacg
    31681 ctgccctcca ggctcacctg taggagcttc tgagcctcaa cggcaaactc cataggcagc
    31741 ttgttgagca cttcctttgc atatccgtta ataattagac caacggcatc ctcagttgaa
    31801 attccacgct ggttgcagta aaaaagctga tcctcaccga tttttgaagt tgtcgcttca
    31861 tgctcggcta tggcggttgg gttgtccact tcgatgtagg gaaaggtatg cgcaccacac
    31921 ctatcaccaa ttagcaagga gtcgcactga gagaagttgc gcgcattatc ggccccttta
    31981 agcaccttaa ccagcccacg gtagctattg ctgccctcac ctgacgaaat tccttttgaa
    32041 acgattcggc tacgggtatt cttgccgatg tgtatcattt ttgttcccgt gtcggcctgc
    32101 tgatggtggt tggttacagc aacggagtaa aattcaccgt aggagttgtc gcccataagt
    32161 atgcagctag gatacttcca tgtaatggcc gaccctgttt caacctgtgt ccacgaaatc
    32221 ttgctgtttt ttcccttgca aatgccacgt ttggttacaa agttgaatat accgcccttc
    32281 ccgtttttgt cgcctggata ccagttctga acagtggaat acttcacctc ggcattttcc
    32341 agggcaacaa tttctaccac tgctgcatga agctggtttt cgtcgcgcat gggtgcagtg
    32401 cacccctcaa ggtaggaaac gtagctatcg tcgtcggcaa ctatcaaggt gcgctcaaac
    32461 tgtccggtat tggcagcatt tattctaaag taggtgctta gctccatggg gcaacgcact
    32521 cccttaggaa tatagcaaaa cgaaccatcg ctgaagacgg cagagttgag ggaggcaaaa
    32581 aagttgtcgg tggagggaac aacagaggcc aggtatttac gcaccaagtc gggatgctcg
    32641 cgaatggctt cgctcattga acaaaaaata atgccatgct cggctaaagt atcccgaaat
    32701 gtcgtcttca ccgaagtgct gtccataact gcatcaacag caactcctga aagcatttgt
    32761 tgctcctcaa ggggaatgcc tagcttgtcg aaagttgcct ttagctcagg gtcaatttcc
    32821 ccgccccctc cggttgaagc atttcgtttt cgcggagcag catagaaaat tatatcctgg
    32881 tagtcaatgg ggggtattgt taaatgagcc catgtgggca tttccatggt ttgccagtgg
    32941 cgaaatgctt ttaagcgaaa ctccagcatc cagtcgggtt cgcctttttt ggacgaaatc
    33001 atcctcacca cctcctcgct gagccctcga gggatagttt cagtgtctat ttgggttaca
    33061 aaaccgtact tgtattccga acctgtaacc tcatgcaaaa ttttatcctg ttccccgttc
    33121 ataccgttgt tcaaacatta tcaggatgta aacaatgcta ctggtgatat gtttaataaa
    33181 taacaagaat gccacgcaac cacgccacaa cttctatatt acccatcaaa cataggttta
    33241 atcgtagcat aaaacaactc caatcggtgg ctgaatcaga agagtgttgc tcctgcaaag
    33301 atatttggct gaaagctaat ttacaaggaa atttgaatgt aaatcgccgg aatacaggtc
    33361 agataggctg gcaaatgtag aattcatccg ttatgggtcg ccccaatggt atgagctaac
    33421 atggcaacaa cttaaagtca tggcttccgg aacagtgcag gaataatagc cgtcttagtt
    33481 catcgggcgt aatctcataa atagcattat agcaggctta tacctttaaa aatttgttct
    33541 aataaaaagt ttataaattg atgacatcct atggccttct tttatacttt tgcgggttca
    33601 aaaaattacc cattttagct taaaataagt tcatgctgtt gttaatgagg ctgttttacc
    33661 agttaaagtc ttgtaatctt taaacaaccg tactggttta acctggcaat tcacccatca
    33721 gtatagatag atacccaaaa ggtcatcgct actccaaata aaatcatgat taaccctaat
    33781 aatcttgata tttgtgcact agtaaagcgt tgggttgcaa gatttagcga gtagatgaaa
    33841 ttctaaggtt caacgtgaag ctgaccgccg gattacattt ttatgatagc aatagtataa
    33901 atgttaggta taaaaattaa aggttgactt atgagaaatg acaaggcagg gaaaatatcg
    33961 aaattaagct ttgtcgggct aatcataacc atgggtattg tgtatggaga tattggcacc
    34021 tccccgcttt acgtgatgcg ggcaatcgcc agcagcgtaa ctaccataga caacgacttc
    34081 atactgggtg cgttgtcctg cattatctgg acgctaaccc ttcaaaccac cgttaagtat
    34141 gttctgatca cccttagagc cgacaacaaa ggtgagggag gcatctactc cctgtttgcg
    34201 ctggtaagga ggaagatgcc aaaggcgtat attattgcca tcatcggggg gagcaccctc
    34261 ctggctgacg gtatcattac acctgcaatt accgtaacat cggccatcga aggtttacaa
    34321 atcgttcaaa agcaacttcc tactattccc attgttctca tcataattac aggacttttt
    34381 gttatccagc agtttggcac taaaaaaatc ggtagctcat ttggccccat aatgacacta
    34441 tggtttttaa cgctggcgat actgggagta aatcagattg ttcagctacc cgtggtgttt
    34501 aaagcgttta atccctacta cgccttgcaa ttaattgtag agcatccatc ggccatgctg
    34561 gtattgggtg cagtatttct ttgtataaca ggggtagaag cgctctactc cgatttgggg
    34621 cattgtgggt tgaaaaacat tcaaattagc tggatattcg taaagtcggc tttaattctt
    34681 aactacctgg ggcaaggtgc ctacctgtta aaaaatggtt ttaacggcaa taatccattt
    34741 ttttcgatta tgccccaatg gttcgtgctt ccaggtatta ttcttgcaac cgcagctgcc
    34801 attattgcaa gtcaggcctt aatatccggt tcattcaccc tgatcagcgt tgcaagctcc
    34861 ctaaacctat ggcccaacat caagagaaaa taccctacca atattaaggg acagatatac
    34921 attccctcgg taaactggtt tctgtttctc ggggtaatct tcgtggtgct cttttttcag
    34981 gaatcgtcca atatggaagc tgcctacgga ctatcggtta accttacgct gatttccaca
    35041 accattctgg ttgctttcta cctgatgtcg aagaaggtaa acccagtcct gattacctta
    35101 ttcagcctgt cattcctttt gattgaaggg gtattcctgt atgcaaacct cctgaagttt
    35161 aagcacggag gctggttgac gctaaccctt gccagccttt tcttcatcgt aaaatacggc
    35221 tggtataacg cccgtaaaat cagaaatcgc catatcacct tcactagtat caacgagtac
    35281 ctacctataa tttgcgacct gagtaaggat aaagaggttc ctaaatacgc aacaaatttg
    35341 gtgtatatga cccgtgctaa caacccagat gaaatcgaat caaaaattat ttactccatt
    35401 ctgaacaagc aacctaagcg tgccgacacc tactggtttt tacatgttga caagctcgac
    35461 gaacctgata cttttgcata cgaggttacg gaaatggtgc ctggaattat cagaaggata
    35521 gatttcaggc tgggttttaa aattgagcca agagttaacc tttactttca ccaggtcctc
    35581 gaagaaatga ctgcgctaaa ggagatagac ctggttagcc attataaatc gctaaggaag
    35641 tataacataa cagcagactt tctcttcatc atgattgacc gtgttccaaa ctacgacatc
    35701 gatttttcac ccttcgaaaa gtttatgatg gtgctttact cgatattccg caggctgggc
    35761 atcagcgagg ttaagtcgct tggactagac agcacgaaca taatcgtaga gaaggttccg
    35821 ctatcaaccc acgtaagcta caaacgaaaa gctattaagc gtaaaatctt ctcataaggg
    35881 gttgaatcct gcccccatac ttttgggtag caagcagtgt ataaacttat cctttcacca
    35941 accacatggg ctaaatcaac cttttggcaa ggctttgggg ggtagcacac atatttcgaa
    36001 aatcaaaacc tgttatgaga accaacccgg gatttttacc cggctcaagg cttccgtatg
    36061 tgctcgaaat acccagtgct tcggctccgt taagggtagc ccattgcaaa acatttgaga
    36121 aagtaagctg tgggaagtgc tgaaggatga tattgatctg gctaagcatg gaaagcgaag
    36181 atgatgaagc caagctgtcc gtgccaatgg caatgttgag gccgctggtt tcgaattcgg
    36241 ggaggttggg tagtttccct tcaattagca ggttcgattc aggacacgta actactgttg
    36301 tttgtgggta tatagcagca agccagctaa gctcctcagc tggagcaaag gtattgtgaa
    36361 taagcatcac cttgcagtcc cgattaacat agcgggacac aactccacgg ggagttagcc
    36421 cttggggctt acaaaaagga attcctgccg actggtacct gtcgctgagg ggaccagtcc
    36481 cgtcttgaag gaactggtat tcggcagcgc tttcggcata gtgaatgcta accagctctt
    36541 tacttttccc gataaattct gaaatcttct cccataaagg ttgtgaaatt gagtagggcg
    36601 aatggggggt taacgaggta gaaccacaaa atttttgctt aaacacttcg taaacttcca
    36661 agtaaccttc aaatatagat tgggcttcgt tggggtttaa cccaaatact tcaataaagt
    36721 tgtggaagta tatatccgat tgaagtttag ccaggagggt gtcggcggag tttgatatat
    36781 cgccaacggc aacagttccg gtttgttcca tttcgtgaat agctcccgct atcgcccgtt
    36841 caaccaactc accatggttt tgaacctctt catccgttcc tctttgctcg gtaatggcct
    36901 taataaaacc cgcaatgccc aagttcttgg gtatcttacc acgaagatgc gagagttcca
    36961 aatggcagtg ggcatttaca aaacccggaa caattacccc gttgtaaaac tccgtggaat
    37021 gaatttcctt tggagaattt ccaggatgaa aaactctcat aataacacca tcgtcatcca
    37081 cctcaacaac tccgtttcga attggtggct ggctgacggg gaaaatatag tttgcagaaa
    37141 acctgtgcat gttactgttg aaccggaatg ggctttcgaa aggcctttat aggtttttta
    37201 tcctttaaat tacgcaccgg gctctttggt gcgggcttgt aggtaacctt tatattgttg
    37261 aaaactgcat gccggttaaa gttttgattc ttattggcat tatcatagag ccagccttta
    37321 agatgcgtgt actggctatc ggtgcagcta aacgtaaagg aatagtggtg aaccactcca
    37381 tccttagggt agagctggac tttagccaca tcacgataat cccgttgaac attatcggct
    37441 ataaaccaaa acgcattcat gcggcactca acagaagagt cgtcgggaaa cagctgggct
    37501 tcaaaagaaa gagtgtaatc accaagccct ttaacaggaa tatcgaagcc tagggagcgg
    37561 ttttgaccat aatcaacagc catgttccag aatgttttat gaggccaaag attttcagaa
    37621 gtatccgtag gggtgttttg gtcttcctct aaatttttag ggttgatact agcttgttct
    37681 tccattttgg atagctccat aataaccctg tcaaatatct catccagctt ttcggtgtgg
    37741 taggcgtagt acttcattga gctgtcaaac tcttgtgttg tatagccata cgattcgatg
    37801 atgggctcgt agtaggcaat ggtgtcccta ccaaaaagag tatgcttttc gggaagaaaa
    37861 gttatgccat cggtaatgta aatctttgaa aggattttaa ccatgtcatt ctcaggaata
    37921 attccggaat ttccacatga caggcaagct ataaccacag ccggcaatcc taccagccaa
    37981 attaccattt tcctcattct acataattta cagctgcaaa tctacttcaa tttaggtgaa
    38041 aatgaaaaaa ccggacaacg gtgtccggca aacatatcct acatgcattc ccgctactta
    38101 attttatcaa acatgtagcc aagcataatt tcgtgagatc cggagttgta cttgctaatg
    38161 cccagaaggc tccagtcgaa cgagtaaccg aacataaact tcctgttcca gttaatgcca
    38221 acaagggctg atacggcgtc cttccagcga agcgaaaggc ctccccaaaa ttggtattct
    38281 ttctgccggt agctgatttt cccgtttagt tcaaactgca tgggactggc aaacatgtac
    38341 ttgattatgg ccgatgtttc cagctccatg tacctgttga ggtttaccca gtaacccccg
    38401 ctaagcatga tatgctgctt taggcggtta acaccgatgg gctcggggta aaacttcaac
    38461 ttttgaccaa aaagctggtg tgccgacaga ccaacgttaa atgaggtgct gtaaaggtaa
    38521 aatcctacag aggcatcggg tgtaaaaaca gattttgtag cttgaataat ggccggatca
    38581 tacatcggat ctggctcttc tcccagggtg agctttgagc cgtcgagctt ataaatcatc
    38641 atgccgaatg aaattccacc ggaaattctc attcccgact ggcttacctg catgttgtag
    38701 gcataggttc ccattatgct tgtccgactt gtaggcccgg taacatcatt cgaaaagtaa
    38761 ccaccccagc ccatatcgtg cttggtaaat ggaccgtaaa aactcatgtt aaccgtgtag
    38821 ggtgcatcgt tgagcccaac ccattgatac cggttgttca acctcatctg gtagtggttt
    38881 aatgtcccag ccactgccgg gttggtcagg tagttattga acatatactg ggagtaatgg
    38941 ggtatttgct gcgaatttac ctgacccgcc attcccagca taataacagt aactagcggg
    39001 taaacaaggt aagctgctgt acgtgttgtt ttcatgcgtt taaacttata taccggtact
    39061 ttgatagctg ctacaaaaaa tatttttaat ttaaacccta tcctaaaatg cctttgtaca
    39121 attcctttaa ttcttttgag ccattaacta taaactcata ggatataaaa attgtatcct
    39181 aaacattaca gcctttactt cacaattgta acagaacctg taatgggttt ccagctggct
    39241 ccctgagcct gaacgttgaa ctttactatg tagtagtagg ttccaatggg cagctcattg
    39301 ccattgcttg ttctaccatt ccatcccttg gccgccttat cgccggtagc cgaccaaacc
    39361 aacgaccccc accggttgaa aatctctatg gataaatcgg ggaacaggta atccttggga
    39421 acaccccaaa catcgtttat cccatctccg tttggcgaga atacgttggc aaaacccaga
    39481 ctggtaacct ccttagtgta aagccgcagg gaatcggtgc aacccatctc ggtggtggca
    39541 atcacatcaa ccaggatgaa atcggttggc tttttggata tcgggtcggt aagtttttgt
    39601 ttcctttctt caggtatttg ttgctttatt tggtcgttga ttagtatgga gctattccag
    39661 ctgtccggcg gattgaacat ttcggcaggt ttccattgaa actccgtggc gtactctacg
    39721 ctgcgtgtaa taacgtcaag gttaacctcc ttacccgtaa gaattgagat gatggtatcc
    39781 tgaacagccg aaatgtaaag gggaacctca agcccaattt tggggagaac gttgaggttt
    39841 attcgggtag agtccttaca cccagagtta ttttctatag cccaaagcaa gaatgatgaa
    39901 ctttcaacaa ggttaacctg gtaatcctga ccccgatgaa ttggatcgcc atcaatatcg
    39961 gggaatttac gccactcgta ggagaagtcg aaggtggtat ctaccgttcc cccttcattt
    40021 ccagatataa ccgaaccatg aagaattaca gggttactat agcaaacggt ggtatcgctc
    40081 ccagcattag taaggaaatt gtaacgggga tttatgggta cctcgtaatc gaaggaatac
    40141 acacagccgc tggtatcctt aaccgtaact gtgtatatgc ctccaccaat aatcattggg
    40201 tctcccgcct tgggaagagt aggaccggtg gttgcgttag catcattcca ctgaaattcc
    40261 aggtcagcaa actggccatt acctcctgtg gaattccgga tgtctattct tccggtatta
    40321 ccggttggtt ttattctaca tagcgccgta tcaataacaa ccggattaag atcaagcatt
    40381 ggtgttcctc tgactgtgaa ggtttgtgaa accctacagt tcagggcatc agtaacggtt
    40441 atggtgtagt ctccaacaac tacgctatca agatcaaccg tagtttgccc attcgaccag
    40501 aggtaggtat aatcgggttg acctccgcta acctgaactc taacaaagcc cgatggatcg
    40561 ttcggacaaa tcagcgaatc atccggtgtt aggccactga taataatttg tggcggttcg
    40621 ttcagggtaa acctaacagg tgggtcgaag ttgtcgggac acccgttgtc atccgtaacg
    40681 ctaaggtcat aaacgccggc aaacaggttt tcaaaaactc cggtagtatt cgaaagggta
    40741 gctgttccgg aaaggttgta ggttaatgtg cccgtgccac cggtagccgt aacgctaata
    40801 atacctattg cttcgccatg gcaggtggga tcaaactgcg tttgctctac cagctgaatt
    40861 ggatttggct caaccagctc gggtacatcc gaaacaacag ttgaacaacc atgctggtcg
    40921 gttatggtaa attcgtggta cccggctgta accggggtag ctgttgtacc caccgaagtt
    40981 ccaccatccc aggtaatcgt ataaacagga gttccgcctg taatgttaaa cagaacgcta
    41041 ccatcagctc caccgtggca ttgaggattt tgtaagctga cgaatgtggc aataagcgaa
    41101 tcgggctcta gtatatcaat ttgataggtg gggaaggtat gcggaacgtt atcggtaacg
    41161 gttatgaggt aggaacctgc cgataacccc tcagctgaaa atactccacc gcctagatcg
    41221 ttaacaggaa tcggcgtcat gtcaatttca ttgcaagcta cggtaaaggg tggaaagcct
    41281 ccggtaatgg taatggtgat aagcccatcg gaacctccat ggcagcttac attgttagag
    41341 gttatgttga tattaactcc gctaatgatg aggtcgcctg tttgaaccat acagccatta
    41401 gcatccatta catcaacatg gtatgttcca gtgcctaagc cagtaaaatt gcctgttcca
    41461 tttgaaattc cattgggttg cagggtgtat gttaaggctc cggttccccc ggtagcagta
    41521 acatggattt cgccatcgtt tgcacctggt gtgctttcct gcaccaccac aacattgtca
    41581 atggtaatct gagcgggttg gttaatggtt ctaagggttg attgcaggca gccattcaca
    41641 tcgtttacca cgatgctgta atcgtttgcc ggcaggttga tgaaaatgtt ggaggggtgg
    41701 taggttgctc cgccgtcaat ggagtacacc agtggtgggg ttcccccgga ggcaataatt
    41761 tccagagaac cattggtatc gccgtagcaa ccggtaacat gggtaactat aacattgtca
    41821 atggatatgg tagatggaga ggtaagggtg attggtgcag tggaaacagg cccgcagcca
    41881 ttggcatcgg ttacatcaac ggtatatgtt ccagcaccta gacccataaa aagtcctgat
    41941 gtattgctta cgttatcggg cataagcgta taggtcagaa ttcctgttcc tccgctggct
    42001 gttaccgtaa tgcttcctgt agtggtgcag gttgggttta ctggagtaac agaacttatc
    42061 acaatggcag gaggctcggt aatggtagca ttttcaccgg taatgcaacc actggcatct
    42121 attacctcaa ccaggtaggt gcttgcggat aaacctgtga agcttcccgt tggttgccat
    42181 gctcctccgt caattctata ctgatagggc agtgtgcctc cagttgccga agcatcgata
    42241 cttccatcag taagacctcc acacgaaata ttggatgttg caagtgatag tataatgggg
    42301 gcaggttcgt aaaccgatac cacctgtgta tcggtgcagc cattggcatc gcgaaccacc
    42361 actgtataat caccaccgct aagtccattc tttacgttgt tcacctcaaa gttgagcccg
    42421 ccatcatacg agtaatcata gggaggtgtg ccacccgagg cgttaatggt gatttggccg
    42481 ttggcatctc cgttgcaaac caggtcaact acggagtagg taaacgtcag ttgggaaggc
    42541 tcagtgatga taaatggacc tgcagaaacc gggccacaac tgttggcatc gttaacttca
    42601 acggtgtatg ttccgggagc tagattggta aataatcctg tttcatttgt agcaataacg
    42661 attaggctgg catcaagcaa gctgaaggtc aacggtgcag ttccacctgt tgcctcaacg
    42721 gtaattgtgc catcatttgc accaaaacag gttatattgg tagatgtttg cgagctaatt
    42781 gccaagagtg ccggctcaac aattgtgagg ctcattccaa taacagagca accgttggca
    42841 tccaaaacgg ctggtatata ggtgttagcc gaaagtcctg tgaataaacc agttgcattt
    42901 ccaaatggag caccgccggt aatactgtaa ttataaggtg gtgttccccc tgcaacttcc
    42961 agcgtaatag ttccatcatt tgccccaaag cacgatatgt cggtcttaac ccttgtggta
    43021 aagtataggg catcgggctg tccaacggta aattcctggg taagcacaca cccattcgca
    43081 tccctaacgt aaagcgtgta ggtgtctgct gttaatcccg aaaagtcacc ggtagcctgg
    43141 taggtaatgc catcaatgga gtactctagc gtccccgttc caccactggc tattgctgaa
    43201 acctcgccgt cggcagcacc aaagcatgag gcatcggtaa cggtcaggtt gtcaataaca
    43261 atctctggag ggcctaagat tacagcaggt ttagttacta tacatccttt tgaatccttc
    43321 acgtaaacgg tataggagcc aacccctaca ttaaatgtat gagttgtggg gaagtaggag
    43381 atgccgtcaa tggaaactgt tttagagcct gtacctccgg atacgccggt tacccatatt
    43441 ttaccattgg tgttgtacca gcaaccggtt acatcggttg tgttaacggt aaaggcaatg
    43501 ggtgtgggct ggctaatgta aatagggtta ccattattag ccacggagat acacccatga
    43561 gaatcgcgaa cttttatgta gtatggattg gctggacctc caggtagtcc ggtaaaatgg
    43621 gggtcggaac cccaaacctc aggtacgtat attgaaaact cgtagcctgt cccagaacct
    43681 cccgccacat tgcttatcag aatttccccc gtgtggtcgc cataacaact tgagatagcg
    43741 gcaacctcgt aggtgaatga aatttcactg ggttccacca tttcaatggt agctggccag
    43801 cttgccgtgc agccgttggc atcgcgtacc acaacatcgt agtacccacc gccgacagta
    43861 aatgtattac ttgcttgaaa agtaacgcca ttatctatgg agtagtccag gctgcctgtt
    43921 cccccgcttg ctgttattgt aataacccca tcggctgcgc caaaacatga aattggaaca
    43981 gtagaaacat cggtgattat aataggatcg ggttcggtga ttacaatatc ctcggtatat
    44041 gccggacacg agttggcatc ggtaatgcta agggtatagg tgctagcggg caggctggca
    44101 aagctggctg gagttggata ggatggcgat tgggtgccga ttaggcttgg cccatcgtat
    44161 aggctgtagg tatagtcggg cgtaccgccg cttccaagtg cttcaatgga accgttggag
    44221 ttgccgttac aaccggtaac cggagtcacc gtttttgagg ctagcccaat agcatcaggt
    44281 tgggttaggg ttacatttcc agctgcctca catcctaagt tatcggttat tgtaacggaa
    44341 tagctaactc ctgctgataa ccctgttgcc gtttgggtgg tttgttctgg ggtggtattc
    44401 cagctgtagc tgaatggggg agctcctcct gataaattaa ccgtagcgct accattggag
    44461 tagccattac aggttggact tgatgatgaa aatcctgaaa ctgaaaactc tgcgtttacg
    44521 gtgatcttta ccacgttgct gaagtaatcg gtgcagggtc cgctgctaac cgccctgcgg
    44581 aaccaagtgt cagcagttag acttccaggt tgatagcttt ctgcattagc accggatatg
    44641 tcgaagaatg gaccagctgc acctgtagtg ctgctctgcc actggtagct ataggttcca
    44701 ttcccgcccg ttggaattga gccggtaagc agttcaggag tggtattata gcatattgtt
    44761 tgatcggcgg ctatggtatt gttcgatacc gatgggagaa ccgtgatggc aactacattg
    44821 ctgaagctaa cgcatgagct ggatgttacc actcttctgt accaggtatt ctggcttaat
    44881 gccgtaggtg agtaattttg agaagttgcc ccgggaatat tgctgaacgg gcctgttgcc
    44941 cctaccgtgc tgctctgcca ttggtaggag aatatgccgc tacctccggt tggtagggag
    45001 cctgtaagcg gtacgggaac actcccaaaa caaatggttt gattagcaga aatagtatta
    45061 ttggcaattt cagggataat tgtaatggta attatattgg agtacagcac accgcacgtg
    45121 ttggtttcca ctctccggta gcaccgggtt tcggtttggc cagcaggcgg gtcgtaggtt
    45181 tgtccggttg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    45241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tactcccatg
    45301 agtacgacca tgttccgtta cctcccgatg gattggtagt gctggttatt gggcttgggt
    45361 cggaattata gcaaagcgat tgattaccgg aaattacgcc cccgttcatt tgtgcgtaaa
    45421 cggtaattgt tagcacgttg gagtaaactg ttccacaagc attagtggct gccctcctgt
    45481 acatggtggt ttgagtcaac cctgcaggga catcataggt taaaccgctg gctccgccaa
    45541 taggggtcca tgcgccagca ccaaccttcg actcccagct gtaggtccat gcgcctgttc
    45601 ccccggcagg cgaagtaaca ttggtaaacg cagcaggatc gccactgtag caaatgctct
    45661 ggtctgcagc cacaactcca ccgcttaatg cgccaaatac cgttaccgtt agttcgttgg
    45721 agtaagccac tccgcaggta ttggttgcct tacgccggta cagggttgtt tgtgtaagcc
    45781 ctgcagggac atcataggtc gagcttgttg cgccgagaat tggtgtccat gagccagcac
    45841 caaccttcga ttcccatgag taggtaaagg taccgtctcc acccgaagca gcggcatcgt
    45901 tgctaaatgc tgcgggatcg ccgttgtagc agatggactg gctggagctt atcaaaccac
    45961 cgtttaaatc agcgtaaaca gtaacggtta tggtgttgga gtaaaccgtt ccacaaccgt
    46021 tggttgcaac ccgccggtac tgggtggttt gcgtcaatcc ggcaggcacg tcgtacgtta
    46081 ggccggttgc cccacttatg ctgttccatg gccctgcccc aactttgtac tcccagccgt
    46141 aggtccaaac cccatcccca ccgcttggtg aggctatgtt gctgaatgaa gccggatcgc
    46201 cgttatagca aatggtttgg tcggagccaa ctgttccgcc attaaggttg gcgtaaacca
    46261 aaacggttgt tgtggcagag ttctcacacc cattagcatc agtaactgtt acggtgtaat
    46321 cgccggagtt atcgggagta acgttggtta tgcttgggtt ctgaagggtt gacgaaaagg
    46381 gggttgttga tgtgcttgtc cagctgtacg aaaccatacc attggggcct gaggtcaggt
    46441 tgatggtacc attgtagcag gcaggtccgt cgttggaggc tgtggcactc ggaagagggt
    46501 tgaccgttac gtagatgtag tttgagtagc tgtcgcatcc atttccattg gttaccacca
    46561 cgttgtatga tcctgataca gtagcgttat aggttgatga ggtagctccg gatatatcag
    46621 catcatccct gcgccattgg aaggagtaac ccgcctcgct gtatgcctcc agctcaactg
    46681 agcctccctg gcaaaaagta gttgttccgt tatttgtaat gcttgcagtt ggggattcgt
    46741 aaactgtaat atcaatatac cctgtaacct cgttgcccac gtgatctttt acagtgcatg
    46801 aaatggtata aataccggga gtagtagttt catcaggagt aagaactgca atatcgcctg
    46861 caggggtaat aaacggagcc ccgccccatg tgtagggtcc ataaggagtt acccctccag
    46921 taggataggc ctttaatacc agcgaagagt tctggcaaac tgttggggga tcgcttgctg
    46981 ggagggtttt tatcgtaact gaaaactgcg agtatgccat accgctggta aacatcagca
    47041 caaaaagtaa tgccgataag catttgggta gggtttggtt cttggtaaaa gttttcatcc
    47101 tgtagagatt tgggtgtaaa ttatgtaaca ataaagatag tcgctcccac atacgaacta
    47161 ttggttttgt tgctaattaa ataagctgcc atttccagcc tagcatgggt taaccggttt
    47221 gctattatca acagcaggcc aactagttaa tttttacgaa acaccaggtc aaaatgttaa
    47281 tatcttgcct taaaattgtt gaaaacttaa atgctacaag cttgcaactt agaaaatttc
    47341 tataatgaat aacgaaagta gagctatttt gctaatttgc aaacctctat gggcataaaa
    47401 aaattaataa tatttattca tttattgcta taatactgat tttctgtatg ttaaaattca
    47461 ttgggcaaag ttgaataatt tttgggtatg ctgaaccttc aacaccataa aacctgatcg
    47521 gcatgtttaa gaaattatta cgagccatct actttgcagc aggtttattg agcttataat
    47581 tagttgtact acaaaccgct taccataaat aataacttac tgtttatcag gcaactatat
    47641 tataatttgg ataacacccg tgcccgaaat cggttactcg tcgggcatgg ctattaaagc
    47701 aagaatacca gcaaaataga ggccaatccg gtaaggtagc ctgtataaac ctgtgaagga
    47761 caatgatcac ctcttaccag cctggcgctg gctaataaac ccgatagaac tacaaccaca
    47821 actagcagat ttagggaggt gaagtagtgg agaatcagcc cggcaattat gaatccggta
    47881 acgcctccaa tacctaccat gtgaaggctt attttccacc agtaggatac ggttgctgca
    47941 ataatcatgg ccagaatgcc tccctgaaca attttgataa agatttccgg aacctgaagg
    48001 cggataagca gcaacagcgt aaaaacgtag ggaattatga ccatgacaat gggaaaaacc
    48061 cgctcgcggt ggtggaaaag ttcaatgctt ttgaccgtcc cgaggcgttt caacagcatc
    48121 agaatgaaaa tggggataat gatagtattt aaaaataggg caataaagag taaccttttg
    48181 tatgaatccg gtagaaatga gatatatgat gcggataggt acagcgttaa gaaggcgtag
    48241 cacatcatga aaagcgggtg gagcagaatg gaaagccaac ggtcgggttt aaaagtcatg
    48301 tttaaggttt aaagttgttt tcttagtcgg gcaaccggaa tgcccagctg ttccctgtac
    48361 tttgcaattg tccggcgtgc aatgggataa cctttttgct ttaaggcctc catcagctcc
    48421 tcgtcggtga ggggattgcc tttatcctca ttaaggatac actcctgaag tattttctta
    48481 atttctcggg ttgaaacctc ttcgcccgca tcgttcataa ggccttcgct aaaaaagtat
    48541 ttcaggggat aaataccgaa atgggtttgg atatacttac tgttggcaac tcgtgatatt
    48601 gtggaaatgt cgagtccggt ggcatcggca atatttttca gaatcatggg tttcagcttc
    48661 gattcgtccc catcacgaaa aaattcctgc tggtagttaa gtattgccct catcgtggtc
    48721 atcagggtgt tttgccgctg cttaagggca tctataaacc agcgtgccga ttcgattttt
    48781 tgccttacaa aagtcacagc ctccttctcc tggctggttg cgctatcctt gctatagcta
    48841 tacgattcaa gcatgtcgga atactcgcgg ctgattctta actcgggaac atttcgggag
    48901 ttgaggttaa gctgaaattc cccgtcaaca tagtcgagga taaaatcggg gataacctgc
    48961 tgaattgact ggttgtaagg atcgctaaat cctcctccgg gttttgggtt tagcttaagt
    49021 atttcgttga tggcatcgcg aagctgttcg tcggagagtg aaagcttctg cgcaatttta
    49081 tcataatgct ttcgggtaaa ttcgtcaaaa tggtacttta aaattttata tgcagtagat
    49141 gcagtggtgc ttccattttc ctttaccctg agctggagca gcaggcactc ctggaggtca
    49201 cgggctccaa ctcccggtgg gtcaaactcc tgaataacgt aaagaatttt ctccagctcg
    49261 tcctcgttgg tatcgaggcc tagggcaaag gcaacatcat cggcaatggc cgacaatttc
    49321 ctgcgcaggt agccgtcctc gtcgatgttg ccaatcaggt aaagagccag ctgcttctca
    49381 cggtcgttaa ggcttgttaa ccccagctgg ctttttaggc tctcctgaaa tgttaccccc
    49441 accgagaacg ggatttcctc ccttttatca tccttcgaca cattgctggc aactagccgg
    49501 tagttgggga tgtcctcgtc gttaaggtaa tcttcaaggg tgaactcatc gtcgttcttg
    49561 tcggtttcgg tatcggaatc gtcgtttacg agcgatgctt cggtatcgtt ttcaggttct
    49621 tcagcatcat gaacctcctc caataccgga ttttcttcaa tttccttttt aattcgctgt
    49681 tcaagcagca tggtaggtac ctccagcagc ttaatcatct gtatttgctg tggagataac
    49741 ttctgaagaa gtttttgttg taatcgctgc tttagcattt tactctaaaa ttcacagttt
    49801 ttgggggttc taggaaatgg aataacatcc ctgatgttgc tcatgccggt aacaaagagc
    49861 accagccttt caaaaccaag tccaaatccg ctatggggtg ctgttccaaa cttacgggta
    49921 tcaaggtacc accaaacatc cttttcggga acgccaagtt cggtaatgcg tgaaaggagc
    49981 ttttggtagt tctcctcgcg ctgtgaacca ccaattattt cgccaatttt tggaaaaagc
    50041 acatccattg ccctgacggt tttcccgtca tcgttttgtt tcatatagaa tgccttgatc
    50101 tccttgggat agtcggttag aatgacgggt ttactaaagt gtttctccac caggtagcgc
    50161 tcatgttccg actgtaggtc tgcaccccag aatacgggga attcccattt ttggtttgcc
    50221 ttctcaagga tttcgacggc gcgggtgtag gttagcctct cgaatttgtt gtcaacaaca
    50281 agctgtaacc tgctaaccag ctcatcgtca tacatctttt ttaggaattc caggtcatcg
    50341 gcacagctag tcagcacgta gcgaataagg tattttagga aatcttcggc cagatccatg
    50401 ttgtcctgaa tatcataaaa ggccatttcg ggctcaatca tccagaactc ggccagatgc
    50461 cggggggtgt ttgaattttc ggctcgaaag gtagggccaa aggtgtaaac ctccgaaaga
    50521 gccattgcac ccagttccgc ctcgagttgt cccgaaacgg taagctttgc ctctttacca
    50581 aagaaatcct cactccaatc aatctttccc tccggggttc gtggaaggtt attggggtcg
    50641 agcgtggtta cgcggaacat ggcccctgca ccctcggcat cagaaccggt aataatagga
    50701 gtatgcaggt agataaatcc cctatcgttg aagtacttgt gaattgcaaa agctaaagca
    50761 tgccgaatgc gaagcacggc gccaaaagta ttcgtacggg gacgcaggtg ggcaatttcc
    50821 ctaagaaact ccatgctatg accctttttc tgaaggggat atgttgccga atcagcttcg
    50881 cccagcacct ccaggtagct ggcctgaatt tcaacccgtt gccctgaacc gggcgattct
    50941 acgagctttc ccctaacacc gatacaagcc ccagtagcta cattttttag ctgatcctcg
    51001 ctaaaaacag ccatgtcaaa aacaacctgt atgctgttga tagtggagcc atcgttaagc
    51061 gcaataaagg tgatactctt gttaccccgc ttggtgcgaa cccatcccat tgcagctacc
    51121 tcagctccta ctgttccgct ctcgagcaaa ttttttacct taaccctttc gctattcctc
    51181 ataatcaaga aaattcaatt tttaatcgaa ctgcaaaatc acgacaaaac tataaaatag
    51241 ttggctatct aagctcgaat ttcgacgatt aagaatgtca aggttgaatt ctttcaatca
    51301 aaatgtctaa aaatagaggg aacagcaacc aaatgcaaat ataaacctcc aaagccggct
    51361 ggtctcgcac aggttggttg tgctatgccg acatcagtaa gcaaacagcc acttttaaaa
    51421 taatgcaacg gctggctaaa aagttaaaaa aatcatatta gctaaacaag ttttatattt
    51481 gcagaaatgc cagctaacca ggattagcct cgaatgaccg aaaaacaagt atttataaat
    51541 ccatataccc aacccgatgt cgctttcatt caatcccaac gcgtttgaca acctaattgt
    51601 ggttggcgat cgagtattaa taaagccgaa gaatcctgat gaacggacta aggccgggct
    51661 gctgttgcca ccaggagttc aggagaagca aaaagctcat agcggttacg ttgtaaaggt
    51721 tggccctggc taccccattc ccacaatagg tgagcttgat gagccatgga aaaaatcgag
    51781 ggaaaatgca cgatacttcc cgcttcaggc gcaggtgggc gaccttgccg tttaccttca
    51841 cgaaaactcc atcgaggtgg aatttaacaa cgagaagtac gttgttgttt cgttttcctc
    51901 cattctactt ctctttcgtg atgaagggct tttggagtag atgcagccat tcgatttttt
    51961 tatcaaaaaa atacattttt tcgattaatg gcttatattt gcaagtgaga tacttgaaat
    52021 gccaatattt attggggttt aaagtttaac ctgtagtttt tccttttaaa acaggactaa
    52081 ttgaactaat agtagctggt gttgaatagt tttatgaagc gtttcaggat gtaatacatt
    52141 taaaccgaat taaaccaatc tgtataacta acagctaaac gttttgaaga tgaaaaaatc
    52201 aaccttaatt ttcaacatta ttctctccct tgccgtggtt gccctcttta tccttcattt
    52261 tactcaaaaa tcgccacgaa accatgtttc cggctctacc ccatcagaag caagtgcagc
    52321 cctttctgaa ggactatcag ttgcctgggt caacatggat acccttctca accactacga
    52381 tatgtacttc gacatgcgga aggagctgga gaacaacagc cgaaacctgg aggcacagct
    52441 gaactcaaaa tcaaaagcct ttgagcagga tgccatggat ttccagaata aggttcaaaa
    52501 ggggctggtt acacgctccg aagcccagca gctgcaaacc tccctggcca accgggagca
    52561 ggaactttac aagctgcgcg acgacatgca gatgcagctt gccgaagaag agcaggttaa
    52621 gctacgccag atacactaca gtattaccga atacctcaag aagtataatg ccgataaggg
    52681 ttaccacatt attcttagca gtaacttcgg cggtccgttg ctttacggtc atccggcact
    52741 ggacataacc agcgaggtga taggcggcat taaccaggaa tatgctgcca accataaaac
    52801 tgacaagtaa tttataacct acctaaacta aacctaaacc aaccgaaacc ctttcgatgg
    52861 gcaaccattt gaaggggttt cgccatttta caacaatgga aatccccgaa tactttcaaa
    52921 acaagaactt ttccccatcc attcaggagc tgccaacaaa aaagttatcc atagtggttg
    52981 taataccttc cttcaatgag gcagatctga aggattcgtt aatggcactt gcgagctgca
    53041 gcaaaccacg tggagatgtt gaggttattg tggtggttaa ccatcccgaa aattcttcgg
    53101 ccgaggtggt aacaacctca gaacaatcgc ttcaggttgt ggctgaggca gatagttgtt
    53161 ggggcagcag cagcttcagg ttttacgcca ttaaagcctt taacctcccc caaaagcagg
    53221 ccggagtggg gttagcacga cagataggta tggatgaagc cgcataccgc ttgttcctag
    53281 ctaaacaacc aggaggcatt attgcctgtt tcgatgccga tgccacctgc gaggtaaact
    53341 acctggttga actggaaaac ttttgggcag ccaaccctga catacatgcc tgctctatcc
    53401 gttttgagca tcccattcag ggtaataatt ttccacagga ggtgtatgat ggcattgctc
    53461 aatacgagct gcatttgcgc tactacaacc aggcattgcg ctacaccgga ttcccttttg
    53521 ccttccacac agtaggctca tccatggcct gcaccgcaga agcttacgtt aagtttggcg
    53581 gcatgaaccg ccaccaggca ggagaggact tctacttcct gcagaaaata attccccatg
    53641 ggcattttgc cgagttaaac actacttgca tttacccttc accccgggcg tcgaacaggg
    53701 tgccctttgg aacgggtaaa tcaatggcca tgttcctgga aaatcccggt aatgaaatcc
    53761 aaacctacca gcttgaatcg ttcttcccgc tacagatgct ctttggtaaa atgcaatcgc
    53821 tctaccagct agatgttggc caaatagact gcttcataga ttcaatgcct gccccattaa
    53881 aactattcct gaagcaaaac aatgcaaccg agaagattta tagcattagc cgtaatacgg
    53941 catctcccgg ttcgttcaga aaaagatttt tcctctggtt tgatgccttt ttgatgttga
    54001 agtacctgaa cttcgttcac aaccgtttct tcaaacggca accggtgaag cacgaggcta
    54061 tcaggcttgc cggtataatt ggccttcggg tagatgaacg ttggccagtt gaaaaaattc
    54121 tggttaaata ccgcgagctg gaggccaata aattcaacgt aaacccatag gggtaaaagg
    54181 taaaaagccg tgagaggtga accgtgaacc tttaatttct ttacccttta gccattactt
    54241 tttatccttt aaactttatc ctttaccctt gccttgtccc tgtgctttgg aaaattccta
    54301 tcgttgcgat gcagttagtg ggggttgcgc tttggaaaac ttactacttg catcgccaac
    54361 caccgccgac cctcatcaac cccgggctta cgcccggggc tatccatgtt acgcctcttc
    54421 gggactaaaa ctatttttta acacgacgtg aatgctgaat ccatcatgta cgggatagtt
    54481 ttagaacccc tagcctgtcc gaagggtgaa t
//
LOCUS       DAOSHM010000013        49723 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_527_length_49723_cov_22.966759, whole genome shotgun sequence.
ACCESSION   DAOSHM010000013 DAOSHM010000000
VERSION     DAOSHM010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 49723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 49723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49723
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_527_length_49723_cov_22.966759"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(42..485)
                     /locus_tag="PK839_03515"
     CDS             complement(42..485)
                     /locus_tag="PK839_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG71837.1"
                     /translation="MKKFLIFAMTILVLISCSTSKKMTSGDYRSPRLKQELIDDNTFK
                     ITEYSQDITYGYTEKNPIMVGGNSEGPKNERRFLNALSGPNGEKIEYYRIGSCCPFKT
                     KNSEWGGMLDKYNVTYTGLEKSLIIYINMYDSDTLKVPVGLKLKY"
     gene            complement(671..1888)
                     /locus_tag="PK839_03520"
     CDS             complement(671..1888)
                     /locus_tag="PK839_03520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG71838.1"
                     /translation="MRRILFFILIIFNTYACLGQKKLNNEISSYVHFLQKNSYSAKEY
                     IMTLWDKYDIVIFCERFHGEVTQYNLLLDIIKDERFISNIGNIYTEIGSITIQQDLNI
                     FLQTNYSNEELRKDQLLNIYRNLTFSPYWEKYNFYHFLSSINMLNNTVQESLKLKIHP
                     LSFEFPGWENFKTSTEYKNWFYEQPNFDSIMAVNFIKQYEIQLKNNVRKKALVILNYR
                     HAFSKDVKNEMQGNSIYIPNTARYIFEKYPQKVANVLLNTWTSLPVNNPDIGDPHVPI
                     QGGKWDASFKYIGIKSMGFDFENSPFGYDTFDLWPFSTTELKYKDFFTGFVFYLPLTE
                     HYELYGIPGLVSNDFREELRRRLEIFNPVWNWDTQGQKSEINKTLKQLNRLQKERYYK
                     LNRKLKLIDKWLN"
     gene            complement(2048..2461)
                     /locus_tag="PK839_03525"
     CDS             complement(2048..2461)
                     /locus_tag="PK839_03525"
                     /inference="COORDINATES: protein motif:HMM:NF025613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4348 domain-containing protein"
                     /protein_id="HOG71839.1"
                     /translation="MKTIITTIVLIMISICTSFSQRTNEDFNIFFFKFSVDSVFQIER
                     IKFPLEFKTWEDPVEIGGDIVTIQIKKEDWKHDYFFMTENYRPQIFDNFEGELRDTDE
                     RLFQWIGIETGVNVKYFFKRIEGKWFLIKKENLGD"
     gene            3237..3734
                     /gene="folK"
                     /locus_tag="PK839_03530"
     CDS             3237..3734
                     /gene="folK"
                     /locus_tag="PK839_03530"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HOG71840.1"
                     /translation="MARVYFTIGGNLGAREKNLEEARNLISARIGTVSKQSAIFESEP
                     WGFSSNDNFLGQVLMVETSLTPAALMLEISYIESKLGREHQGIMYSSRTIDIDVLFYD
                     QLVLFTPELIVPHKHLHKRRFVLEPLNEIAPNFIHPLFSMTVHELLSSCPDECKVWRY
                     QPVGA"
     gene            complement(4011..4634)
                     /locus_tag="PK839_03535"
     CDS             complement(4011..4634)
                     /locus_tag="PK839_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG71841.1"
                     /translation="MKLTHNSLRLMVLLSSFVIAGNANALSLGSSDTCKVDYLSFQSG
                     MIIDRYNSLGVRIFFEYQRDIKKKWQWGISYENSRHLGFAATDQPYELETNLNLFSLN
                     GYYKLNVVKNRLFWTAGLGLGMVHAYWEKPLSYTFSEMEHHNIFGPVANVSLTLNVRI
                     TKRVYIEASPLVVVMPTNRVYFSTMDEEHFNNFFAFTFFPFGVKFRL"
     gene            4926..7670
                     /locus_tag="PK839_03540"
     CDS             4926..7670
                     /locus_tag="PK839_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG71842.1"
                     /translation="MKKVGILITVAALVVGTIAVVVYNWLSNERKKDIEATAALPADA
                     PLVVKIDSYTQISQSLKASNEWKSLLTREDVALVDRMVAITDSMVSVNPVAAGLLKPS
                     AIYAAILPQGDKSNQLLVSLKVPENISISDIRQLIEPLAFGSYTSHVKTYNRTEMLVL
                     TSTSNPQKQAALAIKHGILMVGSREIMVEETINRLESEYTLQSNQKFATINQMAGQGV
                     DASLFINFSKLPVLLTSWLNDNFQANANFVADVGDWGEVDLQLTKSRLTTSGIITTSD
                     SATSFLGLIAAQKPVQPEIPGILPAQTSFYFWLGISDLGRYLEDYRTYLDRSGRLFAY
                     TQALAKIGGKVAMEPEELYKRILLNELCLVCLPSTPSGNVDEWYVVAQNQSSSTTGHL
                     LGSLSGKGGSLPAASINVDDKHSYGVFRSPVPGIHRALFGDLFSVVSDEYYCLIDQQI
                     VFGSSIKSLEFFIKEASRGTTLQNSLLHQDVEKELPEKANYRIYTVPSKAKRLLGNYL
                     SNRMATMLTSSPTGKTGALACQLAGGNRNIVATLTVNPSVKEQVAALPVEWVCQLSNT
                     PTLMPQVVVNHNTGEKEVLVQDKANTLYLIDRFGKTIWKRTVDGQVIGNVTQVDIFNN
                     RKLQLAFSTSSKLYVIDRNGKDVQGFPVIFQSRATNPLMVVDYEGNHSYRFFQACSDR
                     KIYVFDSYGKIVPGWMFGSTETIVSERIGYARVAGLDYIIVFDQNRPYILNRKGEERV
                     KPERFFAKNPNSHYALGKDEKGSYFVVTSDTKGLIRFMYLNGKVKDMALRHFTSSHSF
                     GYFDVTSDGQDDFVVADGEYLLATDHLGKKIFSTRFKTKLQPELYYFKLEQIMGIGVR
                     AASNDENYLVDGSGEIVTGFPIEGCTMFSVAKLSNLSKMYNLIVGVKQGAVVCYTLNK
                     "
     gene            7705..7938
                     /locus_tag="PK839_03545"
     CDS             7705..7938
                     /locus_tag="PK839_03545"
                     /inference="COORDINATES: protein motif:HMM:NF027444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG71843.1"
                     /translation="MFAFLTFLFYFFLTLMIIGFLGRLIFRYWMKRVYRKMNGNANET
                     KKRKTTTRQTREKPSKKIINKDQGEYVDFEEID"
     gene            8114..9067
                     /locus_tag="PK839_03550"
     CDS             8114..9067
                     /locus_tag="PK839_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOG71844.1"
                     /translation="MENVVAGIDIGGTNTVVGLVTKSGEILKETTLSTRLETTFDGFI
                     KILVSEIEKLLMTFEHDYKLVGIGIGAPNSSYDLGAIVDAPNLQWKGILPVCKEITKH
                     TGVNSVVTNDANAAAVGEMLFGDAQGMKNFVILTLGTGLGSGIVVDGRLVLGSEGFAG
                     EFGHIVAKPNGRQCGCGKRGCLETYASATGIRRTAFKMLADSNHPSLLRNITYDKLTS
                     KFITEAAKTGDPLARSAFEYTGLILGTRLADLVAILNPEAIFFFGGLANAGELLLDPT
                     RRYMEEYLFPVFRGKVKLLISGLQNKNAAVLGAAALMWKEL"
     gene            complement(9380..11515)
                     /gene="scpA"
                     /locus_tag="PK839_03555"
     CDS             complement(9380..11515)
                     /gene="scpA"
                     /locus_tag="PK839_03555"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HOG71845.1"
                     /translation="MNPDFKKVRINEVNAPKSPLSYNPEWVTSEKIPVMPAFSEADLE
                     SMEHLDYAAGIPPFLRGPYSTMYVMRPWTIRQYAGFSTAEESNAFYRRNLAAGQMGLS
                     VAFDLPTHRGYDSDHERVVGDVGKAGVAIDSVEDMKILFDGIPLNKMSVSMTMNGAVL
                     PIMAFYIVAALEQGATLEQLSGTIQNDILKEFMVRNTYIYPPEFSMRIIADIFAYTSK
                     LMPKFNSISISGYHIQEAGATADIELAYTLADGLEYIRTGLRAGIDIDAFAPRLSFFW
                     GVGMNHFMEIAKMRAARLLWAKIVKQFNPKNPKSMALRTHSQTSGWSLTEQDPYNNVA
                     RTCVEAMAAALGHTQSLHTNALDEAIALPTDFSARIARNTQIYLQEETNICKVVDPWA
                     GSYYVEYLTNELAHRAWALIQEVEELGGMSKAIETGIPKMKIEEAAARRQARIDSGKE
                     IIVGLNKYRLDKEDPIEILNVDNTAVREAQIRRLNKLKAERNNEEVRLALEAITRCVE
                     TGKGNLLELSVEAAKRRATLGEISMACEKVVGRYKAVIRSISGVYSAESMEDQNYAKA
                     RELCEKFAKLEGRQPRIMIAKMGQDGHDRGAKVVATGYADIGFDVDIGPLFQTPAEAA
                     KQAVENDVHVLGVSSLAAGHKTLVPQVIEELKKLGRDDIMVIVGGVIPPQDYQFLYDH
                     GVVGIFGPGTPVSVAAVKILEILLERVTD"
     gene            complement(11675..13558)
                     /locus_tag="PK839_03560"
     CDS             complement(11675..13558)
                     /locus_tag="PK839_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HOG71846.1"
                     /translation="MVKKSNNERLFAEFPPVSTTEWEAAIAKDLKGADYEKKLIWKTN
                     EGFNVKPYYRSEDLADIQTVHTKPGEFPFVRGNNAKKNPWLIRQDMDVCLDKPTEANG
                     KALEWLQRGVDSVGFKLCSDCEPTFDSISRLLKGIDLNRVEVNFTGGVPGAKALPYIL
                     QYLEKGKFDIRQVRLSFDYSPLSNLTLNGKFCCEKDSSYANLQELVENVKSFPNVKVI
                     GINGNIFHNSGSSSVQELGFSLAMANDYIQFLLQKGYGIDDISPRIRFNFAVGTNYFM
                     EIAKFRAARLLWAKMVEVYKPTIETSARMSIHAETSRWSMTVYDPYINMLRTTTESMS
                     AVLAGVDSLNVLPFNAPYSEPTSFSERIARNQQILLREEAYFGKVADPAAGSYYIERL
                     TLSIAEHAWNLFLKVNEMGGYRDAFIKGYVQERISEVAAKRDANIATRRETILGTNQY
                     PNFNEVADLNVVTAEVVTRQNKLKPADAIAQPLTPYRGAQAFEELRYRTDKSGRRPKV
                     FMLTLGNLTMRRARAQFSSNFFAVAGFEILDNIGFNSVDEGVKTALDSQSEIVVLCSS
                     DDEYSELAPIAFEKLAGKTIFVVAGEPQCKSELESKGISNFISIKSNVLETLKGYQKM
                     LSI"
     gene            13794..14867
                     /gene="amrB"
                     /locus_tag="PK839_03565"
     CDS             13794..14867
                     /gene="amrB"
                     /locus_tag="PK839_03565"
                     /inference="COORDINATES: protein motif:HMM:NF013992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HOG71847.1"
                     /translation="MKKLSIFAIVLASMVACTSSKSPKSVRFFADTVGFASKATQMDS
                     VYARIFRFQGDSLNRKQALPGARLVICPHDDYAYVGWQYPATLRNITAKTVIILGVAH
                     KAKTFNIENKLVFDSFDCWHGPYGDVKVSPIRDELLRLMPADMVVVHDSLQLAEHSVE
                     PMIPFLQRSNPNIEIVSILVPYMPFARISELANSLAQNIYSISSKKHLEWGKDFAFII
                     STDAVHYGDEGWGGNNYAPFGADSAGYAKAVEHEYQIVDSCLAGKATPERIERFYRYT
                     VSDADYREYRWTWCGRYCVPLGLATAIRLSNLSKGSSLEGKLVSYSTSIDHQPLLVED
                     LGMGRTAPASIRHWVGYASLVYK"
     gene            15194..16280
                     /gene="prfB"
                     /locus_tag="PK839_03570"
     CDS             join(15194..15256,15258..16280)
                     /gene="prfB"
                     /locus_tag="PK839_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009132894.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HOG71848.1"
                     /translation="MITAEQVKDIVRRRDALRRHLDIDLKQVELDEEEQKTQYPGFWD
                     NPSEAESQLKKIAGIKIWTNGFSKVNSLVEDLLVLYDFAKQGDATDVELEEHFNSTLK
                     SIEELELKNMLRKEEDNLGAIMKINSGAGGTESMDWAAMLMRMYMRWGERNGYDVKVL
                     DIQEGEEAGIKSATLEFTGEMAYGYLKSENGVHRLVRISPFDSNARRHTSFASVFVSP
                     AIDDRIDVVVNQGDIKWDTYRSSGAGGQNVNKVETGVRLYHIPTGIVIENTESRSQFQ
                     NKENAMRLLKSQLYELELRKRREAQAEIEGSKKKIEWGSQVRNYVLHPYKLVKDLRSN
                     YETSDVNAILDGDLNECIKAYLMQYGE"
     gene            16324..17247
                     /gene="rsgA"
                     /locus_tag="PK839_03575"
     CDS             16324..17247
                     /gene="rsgA"
                     /locus_tag="PK839_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HOG71849.1"
                     /translation="MFLGVVVKSTGNLYRVKLEDGSTVECTVRGKLRLKGSKTTNPLT
                     VGDRVNVETSHEGTAVIVDILPRTNYIIRRSTNLSRESHIIAANIDQAILVVTIIQPE
                     TQTAFIDRYLVTSEAYSIPAILVFNKVDILNAAQTAMMNQFISIYEEVGYKCLCTSAT
                     TGYNLDAFRQLLQGKTSLISGNSGVGKSSLINAIEPTLQLKTSSISAAHLKGKHTTTF
                     SEMFELSFGGYVIDTPGIKGFGLIDIDKKELYHYFPEIFRLKDKCRFYNCTHVHEPGC
                     AVIEAVESGTISQSRYMSYLSIYFDENSKYR"
     gene            17293..18339
                     /gene="mnmH"
                     /locus_tag="PK839_03580"
     CDS             17293..18339
                     /gene="mnmH"
                     /locus_tag="PK839_03580"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF008752.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HOG71850.1"
                     /translation="MEVSSIHINSFLTSRESCPVVDVRTPDEFNRGHIPGAVNIPLFT
                     NEEREQVGTKYVRESRYKALLAGLDLAGPKLTSYLHQLKKVASGKNILVYCWRGGLRS
                     SSMAWLFNLAGYNSKTLAGGYKSYRRFAQQFFTNPYRFIVLGGMTGSGKTELLNRLKT
                     INEQVIDLEALANHKGSAFGWIGQPPQPTTEQFENLLFEQLLKLDPLKPIWIEDESSS
                     IGTIFIPPTIYSRMVKCPTIVVDVAKEERVARLVEDYARFSADDLVLSVTRMTKRMGG
                     DRAKQCIDYIRAGNFKEAIDIALSYYDKTYNYGLMSKTAKPITIKTSTKTENSLSLLL
                     AAKNSIGELSWPKD"
     gene            complement(18329..19171)
                     /locus_tag="PK839_03585"
     CDS             complement(18329..19171)
                     /locus_tag="PK839_03585"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOG71851.1"
                     /translation="METKPQTFVIPWDFTPVAENAFKYAVKLSKVSNEPSQIDLVHVL
                     HSGGSPTKSGKTSEEQVQEMIQADKQRLSTAYSVEINTKLLEGSLFDVITDYASEVHA
                     NMVIMGTHGIKGVQKLTGSWALKVIAGSDVPFLVVQDEPAKDRIFSQLVMSIDFRDEE
                     KEKILWASRMAKQYGSKIHVIVPNSFDSGVKKKISNNLAFAKKQFENVGISWEQHNAA
                     KGKSFQEEIVNLAVDLEADLILIMTAPNLDFTDYVFGAQEQYIIANRSKIPVMCINFG
                     TINP"
     gene            complement(19322..20296)
                     /locus_tag="PK839_03590"
     CDS             complement(19322..20296)
                     /locus_tag="PK839_03590"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOG71852.1"
                     /translation="MFTADQLSAIFSELLQGYSFEKEPRKLYEPIRYVLSLGGKRIRP
                     ILTLAACNMFSDDIEPAKIPALAIEVFHNFTLIHDDIMDNATIRRGKETVHEHWDTNV
                     AILSGDAMSILAYKLLTQTHLEYIQQVMNIFNSSALGVCEGQQLDMDFESREMISRDE
                     YIKMIELKTAVLLKCALQIGATLGNATTRQVELVGQLGTYLGLSFQLQDDLLDAFGEV
                     GAFGKAIGGDIVAGKNTILPVETTALISPEEAGKFIKILKANNLKESDKISMIMDYYN
                     RVDVKAVVEELIAQYQTKAAEIFEKLDIDNNRKTTMHTIMDSLVGRKK"
     gene            complement(20518..21078)
                     /locus_tag="PK839_03595"
     CDS             complement(20518..21078)
                     /locus_tag="PK839_03595"
                     /inference="COORDINATES: protein motif:HMM:NF023238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3332 domain-containing protein"
                     /protein_id="HOG71853.1"
                     /translation="MKKTTMFISIFLVMIMGAVQSSCIGSFKLSKKVLTWNKSLGGKF
                     LNELVYILFWVLPVYEVSMLIDGLILNTIEFWTGSNPVAMNPGEVESKIVEQNGVRYQ
                     VVASQNRFDFTQLDGPQKGQSGALVYSPTTKTWSYEGETLSVELLRLNDDGTTSVLLP
                     EGATVAVDATSGLTASLRSFAGVEVL"
     gene            complement(21232..22104)
                     /gene="atpG"
                     /locus_tag="PK839_03600"
     CDS             complement(21232..22104)
                     /gene="atpG"
                     /locus_tag="PK839_03600"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HOG71854.1"
                     /translation="MANLKEIRTRISSVTSTRKITGAMKMVSAAKFKKAQNNLLRFRP
                     FYEKINHLAADLIKSSVSNESAYMIEREQVRNIGVVLITSNNSMCGAFNANIIKRALV
                     VLNELNRQYPQAKVIFYPLGKTGEEFLVKNKFNVVSCNNAIIEKPSVERTKDFFNLLN
                     SLYLGNELDRIVLVYNSFKNAATQIQKVETLLPLVTPDEQYGSEDLRIVEPDPATLLD
                     RLLPYYLLNSLHSSILESSAAEHGARMTAMHQATENATTIMNNLILEYNKARQASITN
                     EILEIVSGANALSH"
     gene            complement(22243..23823)
                     /gene="atpA"
                     /locus_tag="PK839_03605"
     CDS             complement(22243..23823)
                     /gene="atpA"
                     /locus_tag="PK839_03605"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HOG71855.1"
                     /translation="MADIKPSEISELIKDELADLKYKPKFEEVGFVLQVSDGIARVFG
                     LRNVKSNELVEFESGVKGIALNLEEDNVGVVLLGDTFKVKEGDKVKSLGEIASINVGE
                     GLLGRIINTLGEPIDGKGPIAGKTIQLTLERKAPGVIYRQPVSQPLQTGLKAIDSMIP
                     IGRGQRELIIGDRQTGKTAIAIDTIINQRNNFLKGDPVYCIYVAIGQKGSTVASLART
                     LEEHGAMEYTTIVVATASDPAAMQFYAPFAGATIGEYFRDTGRHALVVYDDLSKQAVS
                     YREVSLLLRRPPGREAYPGDVFYLHSRLLERAAKIINNDSIAANMNDLPEPLRPFVKG
                     GGSLTALPIIETQAGDVSAYIPTNVISITDGQIFLESNLFNNGIRPAINVGISVSRVG
                     GNAQIRSMRKVAGTLKLDQAQFRELEAFAKFGADLDAATISVLEKGSRNVEILKQGQF
                     KPLPVEEQVAIIFCGTAGLLKNIPINRIADFEKDFLERCELTLKEEMKRIAAGEFTDD
                     IAKALTQLVSSVSKNYNA"
     gene            complement(23860..24396)
                     /gene="atpH"
                     /locus_tag="PK839_03610"
     CDS             complement(23860..24396)
                     /gene="atpH"
                     /locus_tag="PK839_03610"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HOG71856.1"
                     /translation="MSSGRVATRYAKGLLKHGNQKGNSPEIFASVQFMIRILDEVKML
                     REFIDNPAFSTDEKCNKIKELVGDQKHTDIVNFIQLLLKKHREEYLYNSLHIFRNLYL
                     RENNILDVVIESVNELDDNVVSRIEAYISSQFGKKCEIKVEVNPALIAGIVILVDGKV
                     IDYSVKGQLEGFRKKLVG"
     gene            complement(24411..24905)
                     /gene="atpF"
                     /locus_tag="PK839_03615"
     CDS             complement(24411..24905)
                     /gene="atpF"
                     /locus_tag="PK839_03615"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HOG71857.1"
                     /translation="MGLVTPDYGLLFWMLLSFSIILYILKKFAWKPIIHALKNREDSI
                     ALSLKQAEQARAEIVKLQEENKALAEKARLEREKEFQETKALRDKLLIEAKEQARLET
                     QKMLDKAREQILSEKEAAKKELHSTIVSMSVEISEKVLRKQLAEKDRQVEYINELLNE
                     MSRN"
     gene            complement(24918..25175)
                     /gene="atpE"
                     /locus_tag="PK839_03620"
     CDS             complement(24918..25175)
                     /gene="atpE"
                     /locus_tag="PK839_03620"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005777530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HOG71858.1"
                     /translation="MTTLLILLQAAQGAALGKIGAAIGAGLAVIGAGMGIGSIGKSAL
                     EAIARQPEAAGDIRSNMIVAAALIEGVAFFALIVCILVIFL"
     gene            complement(25207..26325)
                     /gene="atpB"
                     /locus_tag="PK839_03625"
     CDS             complement(25207..26325)
                     /gene="atpB"
                     /locus_tag="PK839_03625"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HOG71859.1"
                     /translation="MTSFISRYIISAALVVGLVVGVEEVRADDIAGPVETDSTQTEKS
                     KGFNVADFIFEHVGDSYEWHILTVNGHHISIPLPIILYSRTSGLQVFLSNKFHNSTRT
                     YKGFKWEDEGKYAGKIVQLNSAGEVDDANPLPLNFSITKNVLTIFMLGIFLCWLFISI
                     AKQYKKRPNSAPTGIQNMLEPIVLFVRDEIALPSLGEKQYQRFMPFLLTLFFFIWFAN
                     MLGLIPIFPGGANVTGNIAVTMVLAVFTFVVITVNGNKHYWKDIFNAPGVPWFLKLPI
                     PIMPLVEIMGMFTKPIVLMVRLFANILAGHMVGIVFMSLIFIFAGMKFWLGYVAAPIS
                     LIFSIFMTALELLVALIQAYVFTLLSAIYIGMATTEHE"
     gene            complement(26730..27134)
                     /locus_tag="PK839_03630"
     CDS             complement(26730..27134)
                     /locus_tag="PK839_03630"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HOG71860.1"
                     /translation="MAKLNENELTANNINLVGLGTEISGDISSNGDLRIDGTLIGNLS
                     VKGKVVIGETGRIKGELVCKNSDISGQVEGKITVQELLSLKASARVNGDLNIGKLAIE
                     PGCKFTGYCNMLSAEQNFRSDSKLDSGTSLGK"
     gene            27316..28017
                     /locus_tag="PK839_03635"
     CDS             27316..28017
                     /locus_tag="PK839_03635"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF014796.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HOG71861.1"
                     /translation="MRKLIFATFLVGLGTMVQAQTSWLNTKVSEMSFYTLPFEYESLE
                     PIIDKATVEIHYSKHQRSYFDKLISAAKGTEYYNKTLAEIFEEINSVPAAIRNNAGGY
                     FNHILYWDVLKPGNRSNTSKELLNAITLSFGSTDELKRQVTQEATNFFGSGWVWLCAD
                     RNGGLFVCSTPNQDNPLMANAQKIGTPILAIDLWEHSYYLKYQNRRADYVNQLWDAIN
                     WEVVSELYKQTNSKK"
     gene            complement(28099..29499)
                     /locus_tag="PK839_03640"
     CDS             complement(28099..29499)
                     /locus_tag="PK839_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008626571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HOG71862.1"
                     /translation="MMTKMKMTLISLFRGNYYGVKSIINRTFLCFLLVLATEVTQGQI
                     PNGYYSNANGKTGSELKTALFNIIKNHTSISYDQLWTAFKSTDKKPNGKVWDMYSDNP
                     GGTPPYEFTFVTDQCGNYSVEGDCYNREHSFPKSWFNDETPMYSDLFHLYPTDGKVNG
                     QRSNYPYGEVSNPTWTSLNGSKLGPSSYPGYTGVVFEPIDDYKGDFARSYFYMVTRYE
                     DKVDSWSTDMLNGTAYPAFSTWAKNMLLEWNAQDPVSQKEIDRNNVVYNQYQHNRNPF
                     IDHPEYAQLIWGSYTPVSFTSTPITEAQVGTVYTYNITATGTNGVSLTITSTAKPSWA
                     TLTTTGNGAATLSGTPGAANVGSNGVTLTVSDGTTSASQTFTINVSPASIATTSDLPV
                     FRVYPNPVSDVLHIEASEVKMFSVEIFDLIGKTIYTKDKIMEPVEVEMANFPKGIYMV
                     KILANGKTYMVKVVKR"
     gene            complement(29530..30069)
                     /locus_tag="PK839_03645"
     CDS             complement(29530..30069)
                     /locus_tag="PK839_03645"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HOG71863.1"
                     /translation="MEVAKAALRSEIKRQKELVGAERKMDHARKVFGVVESMEEFKQA
                     KTVLAFWSLPDEIYTHDFIMKWAGLKHIALPVMVGDYLELRLFSSKESLVKSSNFSIF
                     EPSKNKLVEPDQVDFAIIPGVAFDMQGNRLGRGKGYYDKLLPKLNKAIKVGVGYNFQL
                     VGQVPVAEHDVMVDRVVCM"
     gene            complement(30112..32991)
                     /locus_tag="PK839_03650"
     CDS             complement(30112..32991)
                     /locus_tag="PK839_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG71864.1"
                     /translation="MRRLIIILSTIFLIQTAAKAQYYQTGVEPGYTKWRQIESGKIRV
                     IYPSEYEDKASSYLNLLLTTDSLTGRDYNLKSKRIDVVLHPNSMLSNGFVAWAPRRME
                     LITFPSTDGYAQPWSSQLALHEMRHVKQMYALNSSTSRLAYYLFGEQVVGLAAGLVPQ
                     WFLEGDAVVTETAHSFSGRGRSATFWQHYRAHFLADRKTYSYNKWLLGSYKNYIPNNY
                     SLGYQLVSFGNFKYGINTWPKVVNHVAKYPFTAIPFYFGVKQNTGLPARKLADSTFAY
                     LIGRWKADTVDRYLSAVNHPVRMPKSYTSYLYPYKLNDSTMLAYRTSLNDIPTFVEIN
                     LNSQKVKELCYPGYLLGRPTYSDSLIVWAEYQPHTRWEYEDFGRIYGLRIKDAQKIEL
                     SGKGKYFSPAINPDDCSVLCISYSPDGNFKLVSIGKKREERELLSFSSGYEVQELSFD
                     RNTRMLYALVVTQQGKEIVSVDINKSFNVVYGPTFDDVNSIYAFDSRVIFSSTDGDVE
                     NIYLHNNLTDSTVKIYNSRYSSRYCSVTKNHELIFSDYTPDGYTIGTSTIDLDNQKND
                     LISNRVNNSYPNFTLIGKGSVVVDTLQISDSVFSSKPYKGAGTLFNVHSWMPFFVNID
                     GTEVTSINPGVMLLSQNLTGTLTSSIGYGYGNSHLFHTDVTYYGFWPVLSVRFNLYDE
                     DAFLYRVTENPVFPNKNRWEAKLNVYLPLQLANSSYQTYLQPFSDLTLSNDYLFSSND
                     SSYHSGIGTVNSGFYFHRLRNMAHRDLQPRMGLVFRLNLVSAPFNKNSLGSLQAANLK
                     IFLPGFLANQGLKFSAAWQNQNLKRYYLGNEISFPRGFSENVSKKYLGASADYIFPFI
                     TPDLSLGALAYIKRISVNLFYDYARNSYLWVNNNNEIAVHDNLTSCGFEIITDFHLFR
                     FRFPFRLVVQQAFTGSNWNPYTNVSLTLDVYGEFWGNKVNRTLGY"
     gene            complement(33026..34276)
                     /locus_tag="PK839_03655"
     CDS             complement(33026..34276)
                     /locus_tag="PK839_03655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG71865.1"
                     /translation="MKFIFQLLIFLILSFGAAADTLTVMHYNLMYYDREYEECTAANN
                     NVDSKDEYLRTIIGYARPDILTVNEVNASVSSVERIRTNVLNINGITKYKRANFSGSF
                     LANMIYFNSEKVELKSQDVIPTSPRETNVYKLYLKTSSLVQGDTIFLYCMITHLKAGS
                     DPSDEDDRTAASAAIMSYLASSNLNGNVLLMGDMNLYSAEEGAFVNFTTPTGINQFRF
                     YDPLSMVGDWHNNYSYRNIHTQSTHTTSDGCHSTGGMDDRFDFILSSSSLTTGNDGLK
                     ITSYKTIAQDGNRFNESLINPANYSVPTDVLSALYNNSDHLPLKMSLYSDFSLHSATK
                     SNLVPKLVFNNPANNKLHVHVPEGISIVTLNLYSITGGKMLSYQGAEPVVDMDISGLS
                     LGIYIAEATLSDGSVSFFKVLKIR"
     gene            complement(34300..34647)
                     /locus_tag="PK839_03660"
     CDS             complement(34300..34647)
                     /locus_tag="PK839_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor"
                     /protein_id="HOG71866.1"
                     /translation="MKREEQDNDKLKFAYSTNPDFKASNEMQPEQESISPEKQKLVVC
                     IERKNRGGKCVTLVKGFRGSNDDLDTLGKQLKSRCGVGGSVKDGEIIIQGELRQKVAA
                     ILRSYGYNVVVSG"
     gene            34912..37971
                     /locus_tag="PK839_03665"
     CDS             34912..37971
                     /locus_tag="PK839_03665"
                     /inference="COORDINATES: protein motif:HMM:NF022481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HOG71867.1"
                     /translation="MGKFKKVNNITGWIVFAISAVVYLLTIEPTASFWDCGEFIATAF
                     KQEVGHPPGAPLFMMIARFFTLFASDVTKVAALVNAMSALASAFTILFLFWTITHLAR
                     KLIIKDAENISTGNLVAVMASGVVGALAYTFSDTFWFSAVEGEVYAMSSFFTAIVFWA
                     MLKWEEVADEPHSNRWLIFIAYMMGLSIGVHLLNLLTIPALAFIYYYKKYKPTRNGIF
                     VALLVSAVILVAILYIIIPGTFKLGSLFELMFVNGFGLPYFSGLIFYFILLFTLIAWG
                     IYNSHKKKKVLLNTVLVSVAAILIGYSSYGMIILRANANTPINENNPSNVFSLINYLN
                     REQYGDRPLFYGQYYNAPVTGSKDKTTRIPKNGKYVETYLKTTYNFDDQFKTIFPRMY
                     SPQGDHVEEYKKWANIEGRPIKVRNQNGEEEIRNVPTFGENLLFFFRYQVGHMYMRYF
                     MWNFVGRQDDVQSNGGIEHGNWISGIKPIDAIFLGNQNELTDNMKNHPSRNTYYFLPL
                     ILGLIGIYYQLFTKRDRRNSFVVFLLFFLTGLAIVVYLNQTPLQPRERDYAYAGSFYA
                     FTIWIGLGVLAVYEWIQKIAPSTASAAAAGMVCLSVPIIMGTQNWDDHDRSGRYTARD
                     FAANYLNSCAPNAILFTNGDNDTFPLWYAQEVEGIRTDVRVVNLSLLGTDWYIDQMKC
                     QCYDSKPLPISMKHEQYVQGTRDIVYIYNRLNDTVELKNLLNFVLSDDKSTKLQIPGE
                     SPIDYIPASRVKVSVNKAAVIKNGTVNLKDSSLIVSSMAWSLKGQYLQKSALAILDII
                     TNNNWERPIYFVSPYGDGDIGIADYLQLEGFAYRLVPIKTSGRDFLSVGRIDTDILYN
                     NLMNKFSWGRMNEPDVFIDYNNQRTAIVLKLRNTFSRLAQQLISEGKKDSALVVLDKI
                     TKLMPHEKYPYDFFMFDIAEAYYKLNQTETANKILDKYADVTLQSLRYQLSLGDKYRD
                     YNNYDLRLNIQTLQELASLADSYGQVELKTKIENGLNQHYSKLVNVK"
     gene            38057..39568
                     /gene="atpD"
                     /locus_tag="PK839_03670"
     CDS             38057..39568
                     /gene="atpD"
                     /locus_tag="PK839_03670"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HOG71868.1"
                     /translation="MAELAGEIVQIIGPVVDVSFENSNGKLPSINEALELRRTDHSVL
                     VLECQQHIGENTIRAVAMDSTDGLSRGMKVKATGKSITMPVGEQVRGRLLNVIGDSID
                     GLSTLNREADYQIHSAPPKFDELRPEKEVLFTGIKVIDLLEPYSKGGKIGLFGGAGVG
                     KTVIIMELINNIAKGYSGMSVFAGVGERTREGNDLLREMIESDVIRYGEEFKQTMKEG
                     GWDLTKVDTDELRKSQATLIFGQMNEPPGARATVALSGLTVAESFRDGGSGEKGNDIL
                     FFIDNIFRFTQAGSEVSALLGRMPSAVGYQPTLSTEMGLMQERITSTKRGSITSVQAI
                     YVPADDLTDPAPATTFAHLDATTVLDRKIAELGIYPAVNPLDSTSRILTPDVVGEEHY
                     NTAQRVKNLLQRYQELQDIIAILGMDELSEEDKLIVHRARRVQRFLSQPFHVAEAFTG
                     TKGVMVNINDTIKGFNMIMDGEVDKYPEAAFNLVGTIEEAIEKGERLLKEAKA"
     gene            39627..39887
                     /gene="atpC"
                     /locus_tag="PK839_03675"
     CDS             39627..39887
                     /gene="atpC"
                     /locus_tag="PK839_03675"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006948533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HOG71869.1"
                     /translation="MVCDLISPERNIKVENVNYVKVPGTKGSFGILKKHAPIVSSLEP
                     GAVKVILDDKTKLLFAIPSGGFVEVTDDRVTIIAEEVLQQPE"
     gene            40240..40983
                     /locus_tag="PK839_03680"
     CDS             40240..40983
                     /locus_tag="PK839_03680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG71870.1"
                     /translation="MKKILLPFPIIILVILTSTLMAKAQQTVVFQASDGLEVTADLYV
                     KGTDLPYIILLHQARYSRGEYREIAPKLVNLGYNCLAVDLRSGGEVNGVVNRTAMLAA
                     GKRIPAEYYNAMSDVEGAIGYVKSKSDKPIVLWGSSYSASLALIAGAKNLRVAAIVAF
                     SPGEYFDQPDFVSSKIGKLAIPVLALSSKAECQPVRELLSGVNPKLVSYYCPTDSGQH
                     GSKALWQSNPSNGDYWMAITMFFSRLNKN"
     gene            complement(41045..46693)
                     /locus_tag="PK839_03685"
     CDS             complement(41045..46693)
                     /locus_tag="PK839_03685"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HOG71871.1"
                     /translation="MKRLVVSIFSLLIFYEVSGQIFYDFENADISGWIQKPAGRWQAS
                     TINPLSGEFSLKHTFDNTGSSTDTIMVNLPSWNVNNGDVTWRMLVRHGYNPSSSNSWW
                     IYLMTGDEIATGTPASGYAVGVNLSGSDDMLKVWRVDNGIPQALLSSTLNWETTIGTA
                     NVGAIEVKRLSDGTFNLKASAGGTFDNLNDYGSAVDVTYAAFKYLSIQYRYTSSADLK
                     LWVDNIGVDYQAANPNDTTTTIYQPMLQIPSGEILSTSTTPSKAVDVLRFVVEDMGSG
                     DGLPTYVKRVAFTNPIPEISQWNSIIAGVLLKGANGEIPIKSSFISNTKLTLEVDSSE
                     MIIPDGLAGEYTLSIFLKSSGITDGTSLQFKIDSIDHEFLASVAGSGFPKVLPHLITS
                     GLFTIRVDATMLKIHEYSHNYIIGKPFALGVAGCDSLGNVDTNFNHDIALNLVQGSGQ
                     LSSLLGLVQPAIGGIAVWNDLIYNKNENIRIEATSQGLSPALSSEIFISFDTTSYAGN
                     PSFQPEAEDILSTKNSAADAVEVMRIKIFDVGGDSASTLLKSLRLTRANLPNMAALNK
                     VLGGILVKSGSTWCGLSDVQVKTSTIDIYFSNGSVCVPEGGNLEIGIWVYLKPTGITD
                     NQTITLYIDPLNPLLTAYDNGTQFSSTFPSGIYSSTFTIRVHATHLKFDQTPARVGVN
                     EAFPVVVKAADINGNTDTDYFGSLGLGLAAGDGHLDVSGGTEGSLQNGIANFIASYSK
                     PGSFALLAMEPTLEDVASPFITCGDADGQVYPLFTGSDTLIFTAQNTNTSAALEIFRL
                     KVSDSGTSDGLPLKVKTICLTAFNPNSASSLEKIIGGFMLRLEEKVIIPIEFSQSEGV
                     FYIQLSENQLEIADGDTIEMGVELYLNPAFLKDNEKFQFYIPSSSHGWVAYDDGTAFC
                     PNFLSYIYGLPCSIEVEADRLAFNSYPFAATPNDCFDVEVISTDCFGNPDANQLGQVT
                     LSENSASGNIEIPDSTEPLSDGIATWSNIKFLNEGRFTLKASHDTLLYAVTPQIFCGY
                     SQMCLIEEAFEGDIPGWGGVESWRVSTISPVAGGSSLVHAGDPGAEKSELVIPLNANA
                     YGRVVEWNVVVKTGSWDPSTDNYFYMVLGSTDSLFNNATTSGYAVGINPSEVDDFLSL
                     WHFEGGKKTNLITTRFDWSEKDEVEIRVTLSPTGILSLWYKPKDYGRMVNGGEVLIQE
                     AIPMKYAGFVYAYTPTRAGQLWLDNLKLCLTEFPPLVKSVRLSSLNSVKVSFSKNVSI
                     TSASIPQNYLVRTAEGEPVAVKSVSIDQSKHDMAVLTTEKLSFGSYTLYISNIEDEQG
                     HFTSDSATFGLGSNGNFGRLVINEIMANPIPSNGLPEYEYIELYNPSADTVFTKGWRI
                     VLNDLQLNLPSDTVLPRTFTLIGGSTAMSEMAVFGKTMGVTSFPSLLNDGMLIKLIDG
                     EGNLISFASYSSDWYNDDSRSDGGYSLECIDASNIAEGKRNWHACMDELGGTPCKENS
                     VVAINPDITPPKVIYLEVLSEDTIRIAFSEPMDSLTITDIENYSLSSENLGVKKIMLQ
                     GNLYDGVKLILNNPMEPGSFYQLAIGGEIVDFSGNPIEESTFKIGLPQIPAAGDLVVN
                     EVLFNPYSGGVDFVEIFNNSQKCIDLKNLFLANRNTDLSLNGVNPAADTSRLIFPAEY
                     AVITVNPQQVKQFYYCENEKAFVQVSSMASLNSDEGYVVLLNANMDVVDELHYTEKLH
                     NPIITNVKGVSLERINPKLPSADASTWHSAAQSVGFATPTFKNSQYAETSMEDGYFTI
                     SPEIISPDGDGKDDFLQLCYSLPAEGYVSNIRIFTSAGVEVFRLANNLLLGREGCLRW
                     DGVNYANRIVPTGIYVVYVEYFNLSGEVKREKRVCVVAER"
     gene            46773..47765
                     /locus_tag="PK839_03690"
     CDS             46773..47765
                     /locus_tag="PK839_03690"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HOG71872.1"
                     /translation="MKVAVVGATGLVGQVMLRVLEQRNFPVTELIPVASEKSVGKTLK
                     FKNEALKVSSLEDAVRQKPSLALFSAGSAVSGEWAPRFAEEGTTVIDNSSCWRMHNEI
                     PLVVPEVNASVLSSSSKIIANPNCSTIQLVVAVNPLHQHFRVKRIVVSTYQSVTGTGV
                     KAVNQLFAERQGIATDMAYPHPIDLNCIPHGGSFLDGGYTTEEQKLIDETRKIIGDPH
                     IMVSPTVVRVPVVGGHSESVNIEFEKDFLLEQVYDILKNSPGVTVQDVPSENTYPMPR
                     MAQGRDDVFVGRIRRDVSKPHCLNLWVVSDNLRKGAATNAVQIAEYLLLQNWLK"
     gene            complement(47833..48453)
                     /locus_tag="PK839_03695"
     CDS             complement(47833..48453)
                     /locus_tag="PK839_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009432681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HOG71873.1"
                     /translation="MAELVARITAWYMGNINYTTITILMAIESSFIPFPSEIVVPPAA
                     FKAAQGELNIYLVILSSTFGAIIGAIFNYFISIWIGRKLIYAFAETKFAHLLMIEPKS
                     IQKAESYFVKHGNSSTFIGRLVPGIRQLISIPAGLARMHFGKFILYTTLGAGLWNIVL
                     ALLGYYIPADLVDRYYKELSWAMIILGAAFVLRLGYKAFGKKRQPT"
     gene            complement(48495..49721)
                     /gene="hflX"
                     /locus_tag="PK839_03700"
     CDS             complement(48495..49721)
                     /gene="hflX"
                     /locus_tag="PK839_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HOG71874.1"
                     /translation="MGKIFSTEKKREKAILVGVIKQGQEETEIKENLVELDFLAETAG
                     ALSIKQFIQRIESPNPSTYIGKGKVIEVLAFISDNEVDMVIFDDELSPTQLRNLEAEL
                     GIKVLDRTNLILDVFARRAKTAYAKTQVELAQYQYLLPRLAGRWTHLERQRGGIGLRG
                     PGETEIETDRRVIRNRIARLKEELGRIDKQMAIQRSNRGELVRVALVGYTNVGKSTIM
                     NLISKSDVFAENKLFATLDTTVRKVVIGNLPFLLSDTVGFIRKLPHQLVEAFKSTLDE
                     VREADLLLHVIDISHPGFEEQINVVNQTLVEIGASDKPTILVFNKIDAYSWNEKGEDD
                     LLPPDKFNTSLSSLQNSWMAKANVQTIFISARLKTNLDQFKKLLYSSVRNIHVGRYPF
                     DNFLYPGDITFDGPKS"
BASE COUNT        14112 a        10937 c        10317 g        14357 t
ORIGIN      
        1 ccactgaaac ccttaatggc tatattgtgt gttggcaaca gttaatattt cagctttaga
       61 ccgactggaa ctttcaatgt gtctgaatca tacatattta tatagataat taggcttttt
      121 tctaatccag tatatgtaac gttgtatttg tctaacattc ctccccattc gctattttta
      181 gttttaaaag ggcaacaact accaattcga taatattcta ttttttcacc atttggtcca
      241 gataaggcat ttaaaaatct tctttcattt ttgggccctt cagaattgcc acctaccata
      301 atgggatttt tttcagtata tccatatgta atgtcttgac tatactcagt aattttaaaa
      361 gtattatcat ctattaattc ttgttttaat cttggacttc tataatctcc gctcgtcatt
      421 tttttagagg ttgagcaaga tataaggact aaaattgtca ttgcaaaaat caaaaatttc
      481 ttcataatcg ttaaattttt acgtgtgtct gtcattttaa ttgttgccaa cggtcggcaa
      541 ttgcttacgc tggggagtaa accgccagca gcgtcatcgc atgttaaatt tttactaaag
      601 tactaaaaac tttctaatct gcaaatcagc tccagtaggc taaaccacct gttaacaaca
      661 gaaatctatt ttaatttaac catttgtcaa tcaattttaa ttttcggttt agtttatagt
      721 atctttcttt ttgtaaccta tttaattgct ttagtgtttt attaatttcg gatttttgtc
      781 cttgtgtgtc ccaattccac acaggattaa aaatctctaa tcttcgtctt aattcttctc
      841 gaaaatcatt agaaactagg ccaggaattc catataattc gtaatgttct gttaagggca
      901 aataaaaaac aaaaccagta aaaaagtctt tatattttag ttcggttgtt gaaaaaggcc
      961 aaaggtcaaa tgtgtcataa ccaaatggtg aattttcgaa gtcaaacccc atacttttaa
     1021 ttccaatata tttgaatgag gcgtcccact tacctccctg tattggaaca tgtgggtcac
     1081 caatatctgg attattaacg ggcaaggatg tccatgtgtt cagtagtacg tttgctactt
     1141 tttgtgggta tttctcaaaa atataccttg cagtattagg tatgtagatt gaatttcctt
     1201 gcatttcatt tttaacatct ttactaaatg catgcctgta gttcaggatt actaaagctt
     1261 tttttcgtac attgtttttt aattgtatct catattgttt aataaaattg accgccataa
     1321 ttgaatcaaa atttggttgt tcataaaacc aatttttgta ttccgtggag gtcttaaaat
     1381 tttcccaacc gggaaactca aaggataatg gatgtatttt tagtttaagt gattcttgaa
     1441 ccgtattgtt taacatgttt attgatgaga gaaagtgata aaaattatat ttctcccaat
     1501 agggagaaaa cgttaaattc ctataaatgt tgagcagttg gtcttttctt agttcttcat
     1561 ttgaataatt ggtttgtaaa aaaatattta aatcctgttg aattgttatg ctcccaattt
     1621 ctgtgtaaat attaccaata tttgatataa agcgctcatc tttaataatg tccagcaata
     1681 aattatattg cgttacttct ccatgaaatc tttcacaaaa aattactata tcatatttgt
     1741 cccaaagagt cattatatac tctttggcag aataagaatt cttttgtaag aagtgaacgt
     1801 aggatgaaat ttcattattt aatttttttt gtcctagaca ggcgtatgta ttaaaaataa
     1861 tgagtataaa aaatagtatt cttctcattc ttttatcatt atggttgcta acgttttcgg
     1921 gtatagcccg ttgcctggtt cggagcgccc acttgtccgg gtacaccaaa cgaacccaaa
     1981 tgagttgaaa cccgcaagag cactttgccg gcaatggaat ttacccggtg ttgggcacag
     2041 tgcttggtta atcaccaaga ttttcttttt ttatcaaaaa ccattttcct tcgattcttt
     2101 taaaaaaata tttaacgtta actccagttt ctattccgat ccattgaaat aatctttcgt
     2161 cagtatccct taattctcct tcgaaattat caaagatctg tggacgataa ttttcagtca
     2221 taaaaaaata gtcatgtttc caatcctctt tttttatttg aatagtcact atatctcctc
     2281 caatctctac tggatcttcc caagtcttga attcaagagg aaattttatt cgttcaattt
     2341 gaaaaactga gtcaacagaa aacttaaaga agaaaatatt aaagtcttca tttgtccttt
     2401 gagaaaagga agtacaaatg gaaatcatga ttaagacaat tgtggtaata atagttttca
     2461 taaaatgtta tgtaacaaga atctcagcat tgtgcccaac tcatttatac acgccactct
     2521 gttgcgcctt atttccatat ttggatggat agacgccata aagttgcgtt ctatttctat
     2581 tcaaaagtag aaaaattatt tatttgataa accaaacttt tgaacttcct tactacaaac
     2641 catcaaaagg cggtttaatt ttttgtagtt tttttcagta acgtgcatgt aaatttctgt
     2701 ggttttagaa tgtttgtgcc cgaaaagttc ctgaataaag tttaaatcta ttccctcgct
     2761 tggtataggt tgccttcggc gagctctccc ctagctggcg gatcggtcag cgtagcgtaa
     2821 ccctcgattg gtgttacttt gggcgcctgt atttgagcat ggggttgcta catcgttgca
     2881 aaagagcagc cctttaacag ccttgcagta agggataatt tgctgtttgg ttgcaggcag
     2941 gaggccatac ttagctacgg ctatgtagca aactttgccg gtaaaaggtt aaattggcca
     3001 taaccaactt ttaaggcagt agatgtgtta ggtgaacatt acaccgaatt cgcctgttta
     3061 acctttgcag ctggtattct ttttgctgtt gtaataatgg gttcttgtaa attaaaaata
     3121 tagctattcg acgaaaacca aacataccat taaaattgtt cgtaaaaatt aaccggtcta
     3181 agaacatttt agtaaaattt caaataagtt tgtaccatat aaactggtaa agggaaatgg
     3241 caagggttta cttcacaatt ggcgggaatc taggtgccag ggagaaaaat ttggaagaag
     3301 cacgcaacct tatttctgca cgaatcggga cagttagcaa gcaatcggca atttttgagt
     3361 ctgaaccttg gggtttctca agcaacgata attttctagg gcaagtgctc atggtcgaaa
     3421 ccagcttaac cccggctgcg ctcatgcttg agatatccta catagagtcg aagctgggac
     3481 gtgagcatca gggaatcatg tattcctcca gaacaataga tattgatgtt cttttctacg
     3541 accagcttgt gcttttcaca cctgaactta ttgttcccca caagcacctg cacaagcgtc
     3601 gctttgtgct tgaaccgctc aacgagattg ctccgaattt cattcatccg cttttctcca
     3661 tgaccgttca cgagcttctt tcatcctgcc ccgacgaatg caaggtctgg cgctaccaac
     3721 ctgtaggtgc ctagctacaa gcataataag ttaaagttag ggtttaagct ggttgtagca
     3781 gtaattacca aggtaaaaaa atccctttac gttaatcgcc attgttggcg cattcctccg
     3841 ttttgaataa cctttggtgt tggcccaaga ggtaaaacca tattttaaac tttccacccc
     3901 gaactattca ttttgtcagc tttgaggcag ctgcccaacc tggtttctgt ttacggttgg
     3961 agataccctg acctgccctt ttcaacctta tgacattatt aaaagtttct ctatagcctg
     4021 aactttaccc caaagggaaa aaatgtaaag gcaaaaaagt tgttgaaatg ctcctcgtcc
     4081 attgtcgaaa agtaaaccct attggtaggc attacaacaa ccagagggga cgcttcgata
     4141 tatactctct ttgtaatcct aacattcagc gtaagactta cattggcaac cggtccaaaa
     4201 atattatgat gctccatttc gctaaatgta tagcttagtg gcttttccca gtatgcatga
     4261 accattccaa gcccaagccc tgctgtccag aaaagcctgt ttttgacaac gttcagctta
     4321 tagtagccgt tcaggctgaa aaggttgagg tttgtttcca actcgtaagg ttgatcggtt
     4381 gcggcaaaac caaggtgcct ggagttctcg tatgaaattc cccattgcca ttttttttta
     4441 atatcacgtt ggtactcaaa aaaaatcctt acccccagcg aattgtagcg gtcaattatc
     4501 attccagact gaaaggataa atagtcaacc ttacaggtgt cgctgctccc aagagataaa
     4561 gcgttagcgt tgccagctat cacaaatgat gagagcagca ccatcagcct taaactatta
     4621 tgcgtcaact tcatatggca tcatcgtgta agttaaaaaa atcaatcaac cggataagtt
     4681 ttgctcttgc tgctacaaat tagtataatc cgcaacaggt ataaatgtaa gaaaaatgtt
     4741 catgcagcta attccatgta ataagaaatc cagctgggac gaggggttaa caggattgag
     4801 taaatcagcc gcagcaaaat gagctgcatt ttctaagaaa ccaagtatct ttttaagatt
     4861 aatcctgact aatttagcac ctttatgtta ccattagtag ctacattaaa aaaagcagct
     4921 gacagatgaa aaaggttggc attttaatta cagttgcggc gttagtggtt ggaacaattg
     4981 ccgttgtagt ttataattgg ctgtcgaacg agcgtaaaaa agatatagag gccaccgctg
     5041 ctttacctgc ggatgcccct ctagttgtaa agattgacag ctacacgcag attagccaat
     5101 cgcttaaggc aagcaatgaa tggaagtcgc tactgacaag agaggatgtt gcccttgttg
     5161 ataggatggt ggccattacc gacagcatgg ttagtgttaa cccggttgca gcaggtctgc
     5221 taaaaccaag cgcgatttat gcagccatcc tgcctcaagg cgacaagtca aatcagcttc
     5281 ttgtttcatt aaaagtaccc gaaaacatct ctataagcga catacgccaa cttatagagc
     5341 cattggcgtt tggcagctat accagccatg ttaaaaccta caataggacc gaaatgctgg
     5401 tattaacatc aacctcgaat ccacagaagc aagcggcttt agcaatcaag cacggcatcc
     5461 taatggttgg cagccgggaa atcatggttg aagaaacgat taaccggctt gaaagcgaat
     5521 atacgctaca aagcaaccaa aaatttgcta ctataaacca aatggccgga cagggggtag
     5581 atgcctccct ttttatcaac ttttcaaaat tacctgttct acttacatcc tggctgaacg
     5641 acaattttca ggcaaatgca aattttgttg ccgatgttgg ggattggggt gaggttgacc
     5701 tccagcttac aaaaagtagg ctaacaacca gtggtataat cacaacatcc gactctgcaa
     5761 cctcatttct gggactaata gccgctcaaa aacccgttca gcccgaaatt ccgggtattc
     5821 tgcccgcaca aaccagcttc tacttctggc tgggcatttc agaccttggt aggtatcttg
     5881 aggattaccg cacctacctt gaccgatcag gaaggctgtt tgcatatacg caggcacttg
     5941 ccaaaattgg tggtaaagtt gcgatggaac ccgaagagct atacaagcgt attctgctca
     6001 atgaactttg tttggtttgc ctcccctcta ctccatccgg caatgttgat gaatggtatg
     6061 ttgtagcaca aaaccaaagc tccagcacaa cagggcacct gcttggcagt ctttcgggga
     6121 aaggcggctc actgcctgct gcgagcatta atgttgacga caagcattcg tatggagttt
     6181 tccggtctcc cgtgcctgga atacaccggg ctctatttgg agaccttttt agtgttgtat
     6241 cggatgaata ctactgctta atagaccagc aaattgtttt tggctcttct ataaaatcgt
     6301 tggaattttt cattaaggaa gcgtcgcggg gaacaaccct tcaaaacagc ttgttacacc
     6361 aggatgtgga aaaggagctg ccggaaaagg cgaactaccg tatttataca gttccttcta
     6421 aagcgaaaag gctattaggc aactatttaa gcaacaggat ggctaccatg ctaacctcat
     6481 ccccaacagg taaaaccggg gcattggcat gccagctggc cggtggtaac cgaaatattg
     6541 tggctaccct tacagttaat ccctctgtaa aagaacaagt tgcagcatta ccagtggagt
     6601 gggtttgcca gctcagcaac acaccaaccc ttatgccaca ggttgtggtt aaccataaca
     6661 ccggcgaaaa ggaagtgctc gttcaggaca aggccaacac gctttacctt attgaccgct
     6721 ttgggaaaac aatctggaaa cgtactgttg acgggcaagt tattggtaat gttacccagg
     6781 ttgatatttt caacaaccgc aagctacagc tggcatttag caccagcagc aagctttacg
     6841 ttattgaccg caacgggaag gatgttcaag gatttccggt cattttccaa tccagagcca
     6901 ccaatcccct catggtggtt gactatgagg gaaaccatag ctaccggttc tttcaggctt
     6961 gcagcgaccg aaaaatttat gtattcgatt cctatggaaa aatcgttccc ggatggatgt
     7021 ttggtagtac ggaaactata gtcagtgaac gaattggtta cgcaagagtt gccggactag
     7081 actatatcat agtttttgac caaaaccgcc catatatact taaccgaaag ggcgaggagc
     7141 gggttaaacc cgaaaggttt tttgcaaaaa accccaatag ccattacgcc ctgggaaaag
     7201 atgagaaagg aagctacttc gtagttacat cggatacaaa aggcttaatc aggttcatgt
     7261 acctcaacgg taaggttaaa gatatggcat tgcgtcattt cacctcatcg cattcctttg
     7321 gatattttga tgttaccagc gatggccaag acgacttcgt tgttgccgat ggtgagtacc
     7381 tccttgccac cgaccatctg ggaaagaaaa tattctccac ccgcttcaaa actaaattgc
     7441 aacccgaact ttactacttt aaacttgaac aaattatggg tattggtgtt agagcggcaa
     7501 gtaatgatga aaactacctg gttgacggat cgggagagat agtaacaggc tttcccattg
     7561 aggggtgtac aatgttttca gttgccaagc tgagcaactt gtccaaaatg tacaacctga
     7621 tagttggcgt aaaacaggga gctgttgtat gctacaccct aaataaatag ctaccttcgc
     7681 acaaaaatta caaaaggtaa tatcatgttt gcatttttaa ccttcctgtt ctactttttt
     7741 ctcaccctga tgatcatcgg atttttgggc cgattaattt ttcggtactg gatgaaaaga
     7801 gtatatcgta agatgaatgg caatgcgaac gaaacaaaaa agcgtaaaac aaccaccagg
     7861 caaacccggg aaaaaccctc aaaaaaaata attaataagg accaggggga gtatgtcgat
     7921 tttgaagaaa tagactaatt tcacccgttt aatttaatcg taattcttaa gttgtattaa
     7981 taaacgctta ctgcttatta gtaattcgac gttatgagcg tattgcgtga aattggtgaa
     8041 actaaaggaa tggtttatgc gggttggcgg gtaagtcttt ctacatttaa aaaaattgat
     8101 tttattagtg attatggaaa atgtagtagc aggaattgat attggcggta caaataccgt
     8161 ggtaggactg gttaccaagt ctggtgaaat actcaaggaa acaacccttt ctacgcggct
     8221 agaaacaact tttgatggtt tcatcaaaat tctggtttcg gaaatcgaaa agctgctcat
     8281 gacgttcgag cacgactaca agttggtagg tataggaatt ggagcaccta acagctcata
     8341 cgatctgggt gctattgttg acgcaccaaa ccttcagtgg aaagggattc tccctgtttg
     8401 taaggagata acaaagcata ctggcgttaa cagcgttgta acaaacgatg caaatgcagc
     8461 agcagttgga gaaatgcttt ttggcgatgc acagggtatg aaaaattttg taattcttac
     8521 ccttggtacg ggtttgggaa gcggcattgt agttgatggc agactggttt taggttctga
     8581 aggttttgca ggagagtttg ggcatattgt agctaaaccc aacggaaggc agtgcggttg
     8641 cggaaaacgt ggctgcctcg aaacctacgc ttctgcaacc ggaattcgcc gaaccgcatt
     8701 taaaatgctg gccgacagca accatccaag cctcctgaga aacattacct acgacaagct
     8761 gacctcaaaa tttatcaccg aagctgcaaa aaccggtgat ccgttggctc gctcggcatt
     8821 tgaatacacc gggcttattc ttggaacccg cttggccgac ctggtggcca ttcttaaccc
     8881 tgaggctatc tttttctttg gaggtcttgc caatgccggg gagctgctac tcgacccaac
     8941 acgtcgctac atggaagagt acttgtttcc ggtatttagg ggaaaggtaa aacttctgat
     9001 ttcagggcta cagaataaaa atgccgctgt cctgggagcc gccgcattga tgtggaagga
     9061 gctataaata acgcttaaat ttttgatttt tttcagccgc ttcttttcga cacctgcggg
     9121 agcataattt ttgcaattga actggtaaaa aagaggttgt tcaagagaat ctatgattcc
     9181 ccttcattaa aagcaaattc aaacatcctg aaaaccacaa aatagtggca tgcctataaa
     9241 gccccattcg ggatgcttca aattcacata attttctaat gatgaaagga catgttcatc
     9301 ttatgcactt taccctcaca caaaaagaac caacagccct aggggttgtt ggttcctaca
     9361 ttaaaaattg ttccaactgt tagtcggtaa ccctctcgag taaaatttca agtattttca
     9421 ccgcagcaac agaaacaggt gtgcctgggc cgaatatacc aacgactcca tggtcgtaaa
     9481 ggaattggta gtcctgtggt ggaataactc ctccaacaat aaccatgatg tcatcacgtc
     9541 ctaatttttt taactcctca attacctgcg gaacaagcgt tttgtggcct gcggccaggc
     9601 tggacactcc gagcacgtgc acatcgtttt caactgcttg cttggccgct tcggctggtg
     9661 tttggaacag ggggccaata tccacatcaa atccaatatc ggcatagccc gtagcaacca
     9721 ccttagcacc acggtcgtgc ccatcttgcc ccattttggc aatcatgatt cgcggctgac
     9781 gaccctcgag tttggcaaac ttttcgcaaa gttcacgagc tttagcgtag ttttgatctt
     9841 ccatagattc agcagagtaa actcctgata ttgaacgaat aacagccttg tagcgtccta
     9901 ccactttttc gcaagccatg gagatttcac caagtgttgc gcgtcttttt gccgcctcaa
     9961 ccgatagctc aagtaggttt ccctttccgg tttcaacaca acgggtaata gcttcaagag
    10021 caagcctaac ctcctcgttg ttacgttcgg cttttagctt gtttaaccgg cgtatttgcg
    10081 cctccctaac tgcagtatta tcaacgttaa gaatttcaat ggggtcctcc ttgtcgaggc
    10141 ggtacttgtt taagccaaca ataatctctt tccccgaatc aatacgagcc tggcgacggg
    10201 cagcagcctc ttcaattttc atttttggaa tgccggtttc aatggccttc gacataccac
    10261 ccaactcttc tacctcttgt attagagccc aagccctgtg ggctagttca tttgtaagat
    10321 actccacata gtaggaaccg gcccaagggt caactacttt gcaaatgttg gtttcctcct
    10381 gaaggtaaat ctgggtgttg cgagcaatgc gagcagaaaa atcggttgga agggctattg
    10441 cctcatccag ggcgttggtg tgtaaacttt gggtatgacc tagcgcagca gccattgctt
    10501 ccacgcacgt tctggccacg ttgttgtaag ggtcctgctc ggttaggctc catcccgagg
    10561 tttggctatg ggttcgtaat gccatagact ttggattttt cgggttaaat tgcttaacaa
    10621 tttttgccca aagcaaacgt gctgccctca tcttggcaat ttccatgaaa tggttcatgc
    10681 caacgcccca gaaaaatgac agcctgggag caaaagcatc aatatcgata cctgcccgca
    10741 gccctgttct gatgtactcc agcccatcgg caagggtgta ggccagctct atgtcggcgg
    10801 tagctcctgc ctcctgaatg tggtacccgg aaatggaaat gctgttgaac ttgggcataa
    10861 gcttcgaggt ataggcaaaa atatcggcta taatcctcat ggagaactcc ggcggataga
    10921 tgtaggtgtt gcgcaccatg aactctttaa gtatatcatt ctgtattgtt ccgctcagct
    10981 gctcaagagt tgctccctgt tctaatgctg caacaatgta aaaggccata attgggagca
    11041 ctgcaccatt catggtcatg gaaaccgaca ttttgttaag gggtatgcca tcgaagagaa
    11101 ttttcatatc ctccaccgaa tcaatggcca cacccgcctt gccaacatcg ccaaccaccc
    11161 tttcatggtc ggaatcgtaa ccacggtggg tgggcaggtc gaatgctacc gataaaccca
    11221 tctgtccggc agcaaggttt ctccgataaa aagcgttcga ctcttcagct gttgaaaatc
    11281 cggcatactg cctgatggtc cagggtctca tcacgtacat ggtgctgtac gggcctctca
    11341 gaaatggtgg aatacctgcc gcatagtcca gatgctccat actttccaaa tcggcttctg
    11401 aaaaagccgg cataaccggt attttttccg atgttaccca ctccggattg tagcttaatg
    11461 gggattttgg ggcatttacc tcattaatcc taactttttt aaaatctggg ttcatccctg
    11521 tagtgaattt actttttacc aatttcgttt taaaaacttt tcaattagtg attatccaac
    11581 ctgaaggctc aaccagaaat tcctaatgta ttatgatata aaaataacgc actttaaacc
    11641 tacaacggat ggctaatcgc cggaaaagca gggtttagat tgacagcatc ttctggtagc
    11701 ctttcagggt ttcaagcaca ttacttttta tgctgatgaa attgctaatc cctttagact
    11761 cgagttcgga tttgcactgc ggttctcccg ccactacgaa aattgtcttt ccggcaagtt
    11821 tctcaaaggc aataggggca agttcagagt actcatcatc ggagctgcat agcaccacta
    11881 tttctgattg tgaatcaagt gctgtcttaa caccctcgtc aacagaatta aagccaatgt
    11941 tgtccagaat ctcaaatccg gcaactgcaa aaaagttaga gctgaattgg gctcttgccc
    12001 gccgcatggt gaggttgcca agggtcaaca taaagacttt gggccgcctt ccgcttttgt
    12061 cggttctgta gcgtagctcc tcgaaagcct gcgctccccg gtacggagta aggggttggg
    12121 ctattgcatc tgcaggttta agcttgtttt gacgggtaac aacctccgct gttactacgt
    12181 tcaaatcggc tacctcgttg aagttggggt actggttggt gcctaaaatt gtttcacgtc
    12241 gggtggctat attggcgtca cgcttagccg caacttcgct aatacgctcc tgaacatacc
    12301 ccttgataaa ggcatcacgg tagccaccca tctcgttaac tttaaggaaa aggttccatg
    12361 catgctcggc aatggaaagg gttaacctct caatgtagta gctccccgcc gcagggtcag
    12421 caactttccc aaaataggcc tcttcgcgta gtaaaatttg ctgattcctt gcaattcttt
    12481 cggaaaatga ggttggctcg gaataggggg cgttgaatgg taaaacgttc agcgaatcaa
    12541 cacctgcaag aacggccgac atgctttctg ttgttgtgcg gagcatattt atgtatgggt
    12601 cgtaaaccgt catgctccac cgacttgttt cggcatgaat actcatgcga gcagatgtct
    12661 caatagttgg cttgtaaacc tcaaccattt ttgcccaaag cagcctggcc gctctaaatt
    12721 tggcaatctc catgaagtag ttggtgccaa cagcaaagtt gaagcgaata cgtggcgaaa
    12781 tatcatctat accataacct ttttggagta gaaactggat atagtcgttg gccattgcca
    12841 gcgaaaaacc caactcctga acggaagacg agcctgaatt gtggaaaata tttccattaa
    12901 tgcctatcac cttaacattg gggaaacttt tgacgttttc aacaagctcc tgcaagttgg
    12961 catagctgga gtccttttcg cagcaaaact taccgttgag tgttaagttg gaaaggggac
    13021 tgtaatcgaa tgagagtcgt acctgacgaa tatcaaactt ccctttttcg aggtactgca
    13081 aaatgtacgg cagcgctttt gctccaggaa ctccccctgt aaagttaacc tctacgcggt
    13141 tcaggtcaat tccctttagc aacctgctaa tagagtcaaa ggttggttcg cagtcgctgc
    13201 aaagcttaaa gcctaccgag tccacccctc gttgtagcca ttccaaggct ttgccgtttg
    13261 cttcggttgg cttatcgagg caaacatcca tatcctgacg tattagccag ggatttttct
    13321 tagcattatt tcccctaacg aagggaaatt ctccgggttt ggtatgaacg gtttgaatat
    13381 ccgctaaatc ctctgaacgg tagtagggct taacattaaa cccctcgttt gttttccaaa
    13441 taagcttctt ttcgtagtct gctcctttca gatccttggc tattgccgcc tcccattcag
    13501 tggttgaaac aggtggaaat tcggcaaaaa gcctttcgtt gttgctcttt tttaccataa
    13561 tcggactagt tggattattg atttgcaaat ttagcaatta ctgaattggt aaaacgctgc
    13621 atagtaacat atttgccttt accatgcttt taagcataat ttgaaagttc tttttatcca
    13681 ttgctattga ggttttaatg gaatattatt tacttgcatc ctggtgaaaa tgtttaaggt
    13741 ttaactattt tctctcatga aattccccac aattttacct ttaaggttga agtatgaaga
    13801 agctatcgat atttgctatc gtgctagcta gcatggtggc ttgtaccagc agcaaaagtc
    13861 ctaaatcggt tcgctttttt gccgatacgg ttggttttgc ctcaaaggca actcagatgg
    13921 actcggtata tgctaggatt ttcaggtttc agggtgatag tctgaataga aagcaagcat
    13981 tgcccggagc caggcttgta atttgccctc acgacgatta cgcctacgta ggctggcagt
    14041 acccagcaac gttaagaaat attaccgcaa aaacagtaat aatccttggc gttgcccaca
    14101 aagctaaaac tttcaacatt gagaataagc tggttttcga cagtttcgac tgctggcatg
    14161 gcccctacgg agatgttaaa gtttcgccaa tcagagatga acttctcagg ctaatgcctg
    14221 ccgatatggt ggtggttcac gatagccttc aactcgctga gcattcggta gagcccatga
    14281 ttccatttct gcagcgcagt aacccgaaca tcgaaattgt ttccattctt gttccgtata
    14341 tgcccttcgc aagaatttcc gagctggcaa atagcttagc ccaaaacatt tatagcattt
    14401 catctaaaaa gcacttggaa tgggggaaag actttgcttt catcatctcg accgatgccg
    14461 ttcattatgg cgacgagggt tggggtggaa ataattatgc cccattcggg gcagattcag
    14521 ccggctatgc caaagcggta gagcatgaat atcaaatagt tgacagctgc ttggccggaa
    14581 aagcaacgcc cgaacgtatt gaaagatttt atcgctacac cgtaagcgat gccgattaca
    14641 gggagtatcg ctggacctgg tgcggcaggt actgcgttcc acttggactg gcaactgcca
    14701 ttaggctttc caacttaagc aaaggttcaa gcctggaggg gaaattggtt tcatactcaa
    14761 ctagcataga ccaccagccg ctattggtag aagatttggg aatgggcaga acggctcctg
    14821 caagcataag acactgggta ggttatgcct cgttggttta caagtagcca ttggaggttt
    14881 gcttagccat tcttttgaat aagctaataa acataggttt aggcaaaata cttgcttgat
    14941 taagtgtgtt attacaccga tggaagaatg gcataaccct catttttttg cagagatcta
    15001 gccttactcc aaaattcggc taccctaact ttttgaaatc ggatttaacc agctccgctc
    15061 tatggcatca taaagaatat actgtattta ctcgagactt actcacagaa aaacaatagg
    15121 gaaaatcctt cttcaaatta ctaatccatt caacctttgc ttcaatatat gctatatttg
    15181 cagaaaaaag tatatgatta ctgccgaaca agttaaagat atagtaaggc gacgggatgc
    15241 gctaaggagg catctttgac atcgatttga agcaagtaga gctggacgag gaagagcaga
    15301 aaactcaata tcccggtttt tgggataacc catcagaggc ggaaagccaa ctaaaaaaaa
    15361 ttgcaggcat caaaatatgg acaaacggat tcagcaaggt taacagcctt gtggaggatt
    15421 tgcttgttct ctacgatttt gccaaacagg gcgacgctac cgacgtggag ctggaagagc
    15481 acttcaattc taccctgaaa agcattgagg agcttgaact caaaaatatg ctacggaagg
    15541 aggaagacaa cctgggggct ataatgaaaa taaattcagg agccggtgga accgaaagca
    15601 tggactgggc tgccatgctg atgcgaatgt acatgcgatg gggagaacga aatggttatg
    15661 atgttaaggt gctggatatc caggaaggcg aagaagccgg aataaaatcg gctactctgg
    15721 aatttacagg tgaaatggcc tatggatacc tgaaaagtga aaatggcgtt caccggttgg
    15781 ttaggatttc cccattcgac agcaatgccc gtcgccacac atcgtttgct tctgtttttg
    15841 tttctcctgc gattgacgat aggatagacg ttgtagttaa ccagggcgat ataaaatggg
    15901 atacttaccg ctcgtcgggt gcgggtggtc agaatgtaaa caaggtagaa acaggtgtta
    15961 gactttacca tatccccaca ggaatagtga ttgaaaacac ggaatcccgt tcccagttcc
    16021 aaaacaagga aaatgccatg cggttgctca aatcccagct ttacgagctt gagctaagga
    16081 agcgtaggga agcacaagcg gaaatcgagg ggagtaagaa aaaaattgaa tggggttcgc
    16141 aggtaagaaa ttacgttcta catccctata agctggttaa agatctccgc tcaaactacg
    16201 agacatccga tgtaaatgcc attctggatg gcgatttaaa cgaatgcatc aaagcttacc
    16261 tgatgcagta cggagaatag ggagaaagta gtgcccatag tgaaataatt tattgttcag
    16321 actatgttct tgggtgttgt agtaaaatca acaggaaatt tataccgggt caagctcgaa
    16381 gatggcagca cggttgaatg tactgttagg ggcaagctcc ggctaaaagg gtctaaaacg
    16441 accaacccgc taacggtagg cgatcgggtg aatgtggaaa caagccatga aggtactgcc
    16501 gtaattgttg acatcctgcc cagaacaaac tacatcattc gccgttcaac caacctttca
    16561 agggagtcgc atatcattgc tgcaaacatc gaccaggcaa tcctggttgt aaccattata
    16621 caacccgaaa cacaaaccgc ttttattgat cgctacctgg ttacatcaga ggcatactca
    16681 atccccgcaa ttctagtttt caataaagtt gacattctca acgctgctca aactgcaatg
    16741 atgaaccagt ttatttcgat ttatgaagaa gtaggttaca aatgcctgtg cacctcagcc
    16801 acaaccggtt acaaccttga cgcattcagg cagcttcttc agggtaaaac cagcttgatt
    16861 tccggaaatt cgggtgtggg aaaatcaagc ctgatcaacg ccattgaacc gacactccag
    16921 ttaaaaacca gctcaatatc cgcagcccat cttaagggca agcacaccac caccttcagc
    16981 gaaatgtttg agcttagttt tggcggatat gtgatagaca cccccggaat taagggtttt
    17041 ggccttatag atatagataa aaaggagctt taccactact tccccgaaat tttcaggtta
    17101 aaagataagt gccgtttcta caactgcaca catgtgcatg agcctggatg cgcggtgata
    17161 gaagcagttg aatcaggaac aataagccaa tcgcgataca tgagctatct aagcatttac
    17221 tttgacgaaa actcaaaata caggtagcag ctatattgtt tcaaaaaaca atatcaaaca
    17281 ctttgccaca cattggaagt aagttcaatt catatcaact cctttttaac ctccagggaa
    17341 agttgccctg ttgttgatgt tagaactccc gatgagttta accgaggtca tattcccggt
    17401 gcagtaaaca tccctctttt taccaacgag gagcgggaac aggttggaac aaaatacgtc
    17461 agggagagcc gctataaggc tctactggcc ggccttgatt tagctggacc caagctcacc
    17521 tcatacctgc atcagcttaa gaaagtcgcc tccggtaaaa atattctggt ttactgctgg
    17581 cggggcgggc tacgtagctc cagcatggca tggcttttca acctcgctgg gtataactcc
    17641 aaaacgctgg caggaggata caagtcgtac cgtaggtttg cgcaacagtt cttcactaat
    17701 ccataccgat ttattgtttt aggtgggatg accggtagcg gcaaaacaga actgctgaat
    17761 cggcttaaga ccataaacga acaggttatt gacctggaag ctctcgccaa ccataagggc
    17821 tcagcattcg gatggattgg tcaaccaccc cagcccacca ctgagcaatt tgagaattta
    17881 cttttcgagc aactcctaaa gctagatccc ttgaaaccta tatggattga agacgaaagc
    17941 agcagcatag gtaccatttt tatccccccg accatttact cccgaatggt aaaatgccca
    18001 acaattgttg ttgatgtggc taaagaagaa cgggttgccc ggctggttga agattatgcc
    18061 cgattttcag cagatgacct ggtgctttcg gtaaccagaa tgacaaaaag aatgggtggc
    18121 gacagggcta aacaatgcat tgactacatc agggcgggta actttaaaga ggcaatagac
    18181 attgccctat catactatga taaaacctat aattatgggt taatgagcaa aactgccaag
    18241 cctataacca ttaaaacttc gaccaaaacc gaaaacagct taagcctcct gcttgctgct
    18301 aaaaattcca ttggggagtt gagctggcct aaggattaat agtcccaaag ttaatgcaca
    18361 ttacaggaat ctttgatcgg ttggctatga tgtattgctc ctgagcccca aacacgtaat
    18421 cggtaaaatc cagattagga gctgtcatta ttagaattaa atctgcttca aggtcaacag
    18481 caaggttcac aatttcctcc tgaaaacttt tccccttggc agcattatgc tgctcccagc
    18541 taatgcctac attttcaaat tgtttcttgg caaaagcaag gttattgcta atcttctttt
    18601 taactcccga gtcgaagctg ttggggacaa taacatgaat tttagagcca tactgtttgg
    18661 ccatcctcga agcccataag attttttcct tctcctcgtc cctgaaatca attgacatca
    18721 ccagctggct gaaaatccta tccttagcgg gttcgtcctg aacaaccaga aaaggaacat
    18781 ctgaaccggc tattaccttt agcgcccagc tgccggttag tttttgcaca cccttaatac
    18841 catgggtccc cataatgacc atgttggcat gaacctcgct ggcgtaatcc gtaattacat
    18901 cgaagagact tccttccagt agctttgtgt taatttcaac cgaataggcg gtgcttaacc
    18961 gttgtttatc tgcctgaatc atttcttgaa cctgctcctc agatgtcttg cccgacttgg
    19021 ttggagagcc tcctgaatgc agcacatgca caaggtctat ctggctgggt tcgttgctca
    19081 ctttcgagag ctttacagca tacttaaaag cattctcggc aacaggtgta aagtcccatg
    19141 gaataacgaa tgtttgaggc ttagtttcca tttcgctaaa ggtttagtat aatctttgtt
    19201 agcaaatatt tatgataaat ttatggcatc tttcagtaat aacaaaattt attgtaataa
    19261 gcttactgca aaaaattatt ggaataaaat aaccaataaa agcagcacac cctttgcaca
    19321 attatttttt tcgtcctacc aggctatcca taatggtatg catggttgtt tttcggttgt
    19381 tgtctatgtc aagtttttca aagatttcag ctgcttttgt ttggtactgg gcaatcaact
    19441 cctcaacaac agctttgaca tcaacccggt tgtagtaatc cataatcatc gatattttat
    19501 cggattcttt caagttgttg gcttttagta ttttaataaa tttacccgct tcttcgggcg
    19561 aaatcagcgc agtagtttct acaggtagga tggtgttttt accggctaca atatcgcctc
    19621 cgattgcctt accaaaagca cctacctcac cgaatgcgtc gagcaaatcg tcctgaagct
    19681 gaaaggatag ccccaagtaa gtgccaagct gtccaacgag ttccacttga cgggtagtgg
    19741 catttcccag ggttgctcca atttgaagag cgcactttaa cagcactgcg gtcttcaact
    19801 ctatcatttt tatgtattca tctctggaaa tcatctccct gctttcaaaa tccatgtcga
    19861 gctgctgccc ctcgcaaacc cccaaagcac tcgagttgaa aatgttcatc acttgctgaa
    19921 tgtattctaa atgggtttgg gtaagcagct tgtaggccag aatagacatt gcatctcctg
    19981 aaagaatggc aacattggta tcccagtgct cgtggacagt ttcttttccc cttcttatgg
    20041 tggcgttatc cattatatcg tcgtgaatca aggtgaagtt atgaaaaacc tctatggcta
    20101 gtgcaggaat tttggcaggc tcaatatcgt ccgagaacat attacatgct gctagcgtaa
    20161 gtataggcct gattcgtttc cctcccaaac ttaaaacata gcgtataggt tcgtagagtt
    20221 tacggggttc tttttcgaag gagtaacctt gcagtaactc tgaaaaaatt gcactcagct
    20281 gatctgcggt gaacataacc aaataaaagg gttagaataa atttaacttc ctgaaaatcc
    20341 tagccaacaa ctatacaacc aatgatttta accagcacta aagcatctct cttagccttt
    20401 ttatattttt ccaacaacac ttcaggctgt ttggaatgaa gtataaaaat accaaatttt
    20461 ttataactaa aaaacccgct tagctggtaa gcgggcttta caaaattcca tgctgtttta
    20521 aagtacttct accccggcaa acgacctaag cgatgccgta agtcctgatg tagcatcaac
    20581 agcaaccgta gcaccttcag gaagcaaaac gctagttgtt ccatcatcat ttagcctcaa
    20641 cagctctacg gagagggttt ccccctcata gctccatgtt ttggtagtag ggctgtaaac
    20701 caacgctccc gattgccctt tttgtgggcc atccagctgg gtaaaatcaa atcggttctg
    20761 gcttgcaacc acttggtacc ttacaccatt ttgctctaca attttcgact caacctcgcc
    20821 cggattcatg gcaacggggt ttgaaccggt ccaaaattct atggtgttta aaattaaccc
    20881 gtctattagc attgacacct cataaacagg tagcacccaa aacagtatgt aaacaagttc
    20941 gtttagaaat ttacccccta aactcttgtt ccatgttaaa acttttttgg atagtttaaa
    21001 actcccgatg caggatgatt gtaccgctcc cataatcatc accaggaaaa tgctaataaa
    21061 cattgttgtt tttttcatga tataaagatt taggtttaaa catcttaaca agtttagttt
    21121 atttatacat aaaatgcaat cattatgtaa cgagaatata aaaaaaattt aacccccatt
    21181 acagagtatg aagttataca agccaaacag taccaaaata aagcaaagag gttagtggct
    21241 caaagcgttt gctccggaga caatttcgag aatttcgtta gtaattgaag cctgtcttgc
    21301 cttgttgtac tccagaatga gattattcat aatggtagtg gcattttcgg tagcctggtg
    21361 catggctgtc attcttgcac catgctcagc ggcagaactt tcgagtatgg atgaatgtaa
    21421 tgagttaagc aggtagtacg gcagcaacct gtcaagtaga gtagccggat cgggctcaac
    21481 aattcgaaga tcttcagatc catattgctc atcaggtgtc actaagggta aaagggtttc
    21541 aaccttttga atttgagttg ctgcattctt aaaactattg taaaccagca caatcctatc
    21601 aagctcgttg cctaggtaga ggctattaag caaattaaaa aaatccttgg ttcgttcaac
    21661 cgatggcttt tcaattatgg cattattgca tgaaactacg ttaaatttgt ttttcaccaa
    21721 aaattcttca cctgttttcc caagagggta aaaaatcact tttgcttggg ggtattgacg
    21781 gttaagttca ttgagcacaa ccaaagccct tttaataatg ttggcattga atgctccgca
    21841 catactgttg ttggaggtaa taagcacaac tccgatgtta cggacttgct ccctttctat
    21901 catataagca gattcgtttg aaacgcttga ttttatcaaa tctgccgcca gatggttgat
    21961 tttttcataa aacggcctga agcgcaacag gttattctgg gcttttttaa atttagcagc
    22021 cgataccatt ttcatagcac cggtaatttt ccttgttgaa gttaccgatg aaattcttgt
    22081 tcgtatctcc ttaaggttag ccatttttac ttacactaaa tttaatgacc agcagtataa
    22141 cattgtaaag cggttcgtaa taaggccatt ctggggtaac cctgtaaatg gttaccccac
    22201 aatgaaaatt aacagctcca cgtggcaccg tagtccattt ggctaggcgt tatagttttt
    22261 tgaaaccgag cttacgagct gggtaagcgc cttagcaata tcgtcagtaa actcccctgc
    22321 ggcaattctc ttcatttcct cctttagggt gagctcacag cgttccagaa aatccttctc
    22381 aaaatcggct atccggttaa tgggaatatt tttcaaaaga ccggctgtgc cgcaaaaaat
    22441 aatggcaacc tgctcctcaa ccggtagggg tttaaactgc ccctgcttaa ggatctcgac
    22501 attcctcgaa cctttttcca acacggaaat ggtagccgca tcaagatcgg ctccgaactt
    22561 ggcaaatgcc tccagctccc tgaattgcgc ctgatcaagt ttcagtgtcc cggcaacttt
    22621 cctcattgac cttatttgtg cgtttccacc aacccttgat accgaaatac ccacattaat
    22681 cgcaggtcta attccattat taaacaaatt tgactccaga aaaatctgac cgtccgttat
    22741 ggatataaca tttgtaggaa tatatgccga cacgtcgcct gcctgggttt cgataattgg
    22801 aagagcggtt aacgaacccc cacctttcac aaaaggcctt aacggttccg gcaagtcatt
    22861 catgtttgca gctatggagt cgttgttgat aatttttgca gcgcgctcaa gcagccgtga
    22921 gtgaagataa aaaacatcac caggatatgc ctccctaccg ggtggtctgc gaagaagtag
    22981 agaaacttca cggtatgaaa cagcttgctt ggacaagtcg tcatacacca ccagggcgtg
    23041 tcttccggta tctctgaagt actcgccaat ggttgcaccg gcaaaaggtg catagaattg
    23101 catggccgca gggtcagaag ctgttgccac aacaattgtg gtgtactcca ttgcaccatg
    23161 ctcttcgagc gttctggcca gactggcaac ggttgacccc ttctggccaa tggccacata
    23221 aatgcagtaa accggatcgc cctttaggaa gttgttgcgc tggttgataa tggtgtcaat
    23281 ggcaatggca gtcttccccg tttgccggtc accaattata agttctcgct gtcccctacc
    23341 aataggaatc atcgaatcaa tagcctttag tcctgtttga agtggctgag aaacgggctg
    23401 tctataaatg acacctggcg ctttcctttc tagtgtaagt tggattgttt tcccagcaat
    23461 agggccctta ccatcaatag gctcaccaag ggtgttaata atgcggccaa gcaatccttc
    23521 tcctacgtta atagaggcaa tttctcctaa actctttacc ttatctcctt ctttcacctt
    23581 gaaggtgtct cccaacagca caactcctac gttatcctct tcgaggttaa gcgcaatacc
    23641 cttaactcct gattcaaatt ccaccagctc gtttgatttt acattcctca aaccaaacac
    23701 gcgggcaatt ccatcgctaa cttgaagcac aaatccaacc tcttcaaact tgggtttata
    23761 cttcaaatca gcaagttcat ctttgattaa ctccgatatt tccgaaggtt taatatctgc
    23821 cattgcaatc aatgtttgtg ttcatccagt ataacctcac tatccaacca actttttcct
    23881 aaacccttca agttgtcctt tcacactata gtcaatgact ttaccatcaa ccaatattac
    23941 aatgcccgca attaatgctg gattaacctc aacttttatt tcgcactttt tgccaaattg
    24001 cgaacttatg taagcttcaa tcctactgac aacgttatcg tccaactcat taaccgattc
    24061 tataaccaca tccaggatgt tgttttcgcg gaggtaaagg tttcgaaata tatgtaacga
    24121 gttgtaaaga tattcttcac gatgcttttt aagcagcagc tggataaagt taactatatc
    24181 tgtatgtttt tggtccccaa ccagctcctt aattttatta catttctcat cggtgctaaa
    24241 agcagggttg tctataaatt ccctgagcat tttaacctca tccagaatcc taatcatgaa
    24301 ctggacgctg gcaaatattt cgggcgaatt tcccttttgg ttgccatgct ttagcaatcc
    24361 cttggcatat ctggttgcaa cccttccgct actcataatc tacaaagcaa tcaattcctc
    24421 gacatctcgt taagcagctc attaatgtac tccacctgcc ggtctttctc ggcaagctgt
    24481 tttctaagaa ctttctccga aatctccacc gacatggaga cgatagtgga gtggagctct
    24541 tttttggctg cttctttttc actaagtatt tgttcgcggg ctttatctaa cattttctgg
    24601 gtttcgagcc gagcctgttc tttggcttcg atgagcaact tgtcgcgaag ggcttttgtt
    24661 tcctgaaact ccttttcccg ctccaatctt gccttttctg ccaaagcctt gttttcttcc
    24721 tgaagtttta caatttctgc tctggcttgc tctgcctgct taagcgaaag agcaatggaa
    24781 tcctccctat tcttaagagc atgaattatt ggtttccatg caaacttttt aagaatgtag
    24841 agaattatgg aaaaggatag aagcatccag aaaagcagtc cgtaatcggg ggttaccaag
    24901 cccatagctt acaaatttta aaggaatata accagaatac aaacaatgag ggcaaaaaat
    24961 gccacccctt caataagcgc agcggcaaca atcatgtttg aacggatatc tccggcagct
    25021 tcgggttggc gggcaattgc ttccagcgca gattttccga tgctaccaat tcccatgcct
    25081 gcacctataa cagcaagccc tgccccaata gcggctccaa ttttccctaa tgctgcaccc
    25141 tgggcagctt gtaaaagaat tagtaatgtt gtcatagtta taagatttat aaaaaatgtt
    25201 agttagctat tcgtgttctg ttgtggccat tcctatatat atggctgaca gcaaggtgaa
    25261 tacatacgct tggattagag caaccagcag ctcaagagca gtcataaata tagaaaaaat
    25321 aagcgaaatg ggtgctgcta cataccctaa ccaaaatttc attcctgcaa agataaagat
    25381 taggctcata aaaacaattc caaccatatg acctgccaaa atgttggcga atagtcggac
    25441 catcagcaca ataggcttgg taaacatacc cataatttca acaagcggca tgatgggaat
    25501 gggaagcttt aagaaccacg gaactcctgg tgcattaaag atatctttcc agtaatgctt
    25561 gtttccattt actgtaatta ccacaaaggt aaaaacggcc aacaccatgg taacggcaat
    25621 attacctgtt acgttagctc ctccgggaaa tattggaata agacccagca tgttggcaaa
    25681 ccaaatgaaa aagaataggg ttagcaggaa gggcatgaat cgctggtact gcttttcacc
    25741 taacgagggt aaggcgattt cgtcacgaac aaatagcaca atgggttcca gcatattttg
    25801 tatgcctgtt ggagccgaat taggcctttt tttatactgt ttggcgatag aaataaataa
    25861 ccagcagaga aaaatgccaa gcatgaagat ggtgagcaca ttttttgtaa ttgaaaaatt
    25921 aaggggcaga gggtttgcat catctacctc acctgcactg ttgagctgaa caatttttcc
    25981 agcatacttg ccctcgtctt cccacttaaa gcctttatag gttcgggtgg aattatggaa
    26041 tttgttcgat aagaaaacct gcaatccgct agttctgcta taaagtatta taggaagagg
    26101 aatgcttata tgatgtccat tgacagtaag aatatgccac tcgtaagaat cgccaacatg
    26161 ctcaaaaatg aaatcggcta cattaaaacc cttcgatttc tccgtttggg tagaatccgt
    26221 ttcaacaggt cctgcaatat catctgcacg aacttcttca accccgacaa ccaacccaac
    26281 taccagagct gcgctaataa tatatcttga aatgaagctg gtcattttca aatctacctt
    26341 tttttaatca accttgtaag cattaccacc tcatgggtta agagcacaaa gtatgtggtc
    26401 aaatatagtg atattaatac taccggtttc agcaagccgg caagaagaat tactacaaag
    26461 attgcaacat tgataaaaag cttagctgtg ctaaacaata aatatttttg gttaaagttt
    26521 ttgctgtcct ttgaaaagaa tcgcgctagc tgcaggtaaa aaattatact tacaacggct
    26581 gggaaaaatg gaagcatcgt agaaagaacc ccgaccggtt gcttgtaaat ataaacaaaa
    26641 taaaggttga taataaccga taccactatc gtgattacta ccacctgtgc tatcatccta
    26701 tgaaaaggat tacccatttt tcagtaggcc tacttaccta aagatgtccc ggagtcaagt
    26761 ttgctatctg acctgaaatt ctgctccgct gaaagcatat tgcaataccc tgtaaacttg
    26821 cagccgggtt caatagccag cttccctatg tttaaatcgc cgtttactcg tgctgatgcc
    26881 ttcagcgata acaactcctg tactgtaatc ttaccctcaa cctgtccgga aatatcagag
    26941 ttcttgcaaa ccagctcacc tttaatcctc cctgtctcgc caattaccac tttccccttt
    27001 accgataggt ttccgattag tgtaccgtca atccttaaat cgccgttact ggaaatatct
    27061 ccgctaattt cggttcctag gccaaccagg ttaatgttgt tggcggttag ttcgttttca
    27121 ttaagttttg ccatttccaa ctattttgag ttcaacatat ctcctttaag gcagcaaata
    27181 tataaattta tcaaagctat tgttaaattt ttactattgt ttaacccatt tttttcggct
    27241 tgaataattg tacaccattc tcatgaactt aatttcagcc cacatgttta acgagcatac
    27301 caaaaattaa ttactatgag aaagttaatt tttgctacct ttttggttgg cttgggaact
    27361 atggttcaag cccaaacatc gtggctaaac actaaagtgt cggagatgag tttttacacc
    27421 ttgccctttg aatatgaatc gctggaaccc attattgaca aggctactgt ggaaattcat
    27481 tactccaagc atcagcgctc ctacttcgat aagttaattt cggctgctaa aggaacggaa
    27541 tattataata aaaccctagc cgaaattttc gaagaaatta actcggttcc tgctgctata
    27601 cgcaacaatg ctggtggcta ctttaatcac atcctctatt gggatgttct aaaacccgga
    27661 aatcgtagta acaccagtaa agagttattg aatgctatta ctcttagttt tggttctact
    27721 gacgagttga aacgacaggt aacacaggaa gcaaccaatt tttttggaag cggatgggtt
    27781 tggctttgtg ctgataggaa tggcgggctc tttgtatgct caacgcccaa ccaggacaac
    27841 ccccttatgg caaatgccca aaaaatcgga acgccaattc tggcaattga tctttgggaa
    27901 cattcgtact acctgaaata ccagaatcga agggctgatt atgtcaatca attatgggat
    27961 gccattaact gggaggttgt ttctgaactt tacaagcaga ccaattctaa gaaataagcg
    28021 caaaatgcct gatagaataa caaacatggg ggttaaatag cccccatgtt tgttatagaa
    28081 aaccggaata acagtggttt acctctttac caccttcacc atgtaggttt tgccattagc
    28141 caaaattttt accatgtaga tacccttggg gaagttggcc atttcaacct caacgggctc
    28201 catgatttta tccttagtat aaatcgtttt acctatcaga tcaaaaattt ctaccgagaa
    28261 catttttacc tctgatgctt ctatatgcag aacgtctgat actggattcg ggtaaaccct
    28321 aaatactggc aaatcggaag ttgtagcaat gcttgcaggc gaaacattaa tggtgaatgt
    28381 ttgacttgca gaagttgttc catcacttac agttaaggtt actccgttcg aacccacatt
    28441 ggcagcgcct ggcgttccgg aaagtgttgc agctccattt ccagttgttg ttagggttgc
    28501 ccatgatggt ttagctgtag aagtaatcgt aagagacact ccattagtac cggttgcagt
    28561 aatattgtat gtataaacag tcccaacctg cgcttcggta ataggagtgc tggtaaaaga
    28621 aaccggtgtg tagctgcccc agatcagctg tgcatattcg ggatgatcaa taaacgggtt
    28681 gcggttgtgc tggtactggt tgtacaccac gttattcctg tctatctcct tttggctaac
    28741 agggtcctgt gcgttccatt ccagcagcat gtttttggcc catgttgaaa atgctggata
    28801 cgcagtacca ttcagcatgt cggtgctcca actgtcaacc ttgtcctcat agcgcgtaac
    28861 catgtagaag tagctccggg caaagtcgcc cttgtagtcg tctattggct caaaaacaac
    28921 ccccgtgtag ccgggatatg aacttggccc cagcttgctt ccattgagcg atgtccatgt
    28981 ggggttagat acctctccgt aggggtaatt gctcctttgc ccgttaacct taccatcggt
    29041 ggggtaaagg tgaaagaggt cagagtacat gggagtttca tcattgaacc aacttttagg
    29101 aaacgaatgc tcccggttgt agcagtcccc ttcaacgctg tagtttccgc attggtcggt
    29161 aacaaaggta aactcgtagg gtggagtacc cccgggatta tcggagtaca tgtcccacac
    29221 cttgccattt ggcttcttgt cggtgctttt aaatgccgtc caaagctggt cgtacgaaat
    29281 ggatgtgtgg ttctttatta tgttgaagag tgcggtcttc agctcggaac cggttttccc
    29341 attggcgtta gagtagtaac cattgggaat ttgaccttgt gtaacctccg tagcaagcac
    29401 aagcaggaaa cacagaaacg ttctattgat aatagatttc actccgtaat aattccccct
    29461 aaataggctg attaacgtca ttttcatctt cgtcatcatc ttaccaaaac ttatatattt
    29521 acagaaaaac tacatacaca ccaccctatc aaccataacg tcatgctctg cgacgggaac
    29581 ttgccccacc agctggaagt tgtatccaac gcctacctta atagccttgt taagcttggg
    29641 taaaagctta tcgtaataac ccttacccct gcccaaccgg tttccttgca tatcaaatgc
    29701 cacaccagga ataatggcaa aatcaacctg gtctggctct accaacttgt tttttgaggg
    29761 ttcaaagata ctgaaatttg aactttttac cagactctcc ttactgctga aaagccttaa
    29821 ctccagatag tctccaacca taacgggcaa cgcaatatgc tttaaacctg cccatttcat
    29881 aataaaatcg tgggtatata tttcgtcggg aagcgaccag aacgccagaa ctgttttcgc
    29941 ctgcttaaac tcctccatag attcaacgac accaaaaacc tttcttgcat gatccatttt
    30001 tctctcagca ccaactagtt ccttttgccg ttttatttca cttcgtaatg ctgcttttgc
    30061 tacttccatt tttattagtt gcttttgata ttgtggttgt atatacgtaa gctagtaccc
    30121 caaggttcta ttaactttgt tgccccaaaa ttctccgtaa acatccaatg ttaagctaac
    30181 atttgtgtaa gggttccaat tgctccctgt aaaggcttgc tgaacaacca ggcggaacgg
    30241 gaatcgaaat ctaaacaggt ggaagtcggt gatgatctcg aaaccacacg aggtgaggtt
    30301 atcgtgcaca gcaatttcat tgttgttgtt cacccatagg taggaattac gggcatagtc
    30361 gtaaaatagg ttcaccgaaa ttcgtttgat gtaagccagt gcccccaagg aaaggtcggg
    30421 ggttataaaa ggaaaaatat aatcggctga tgctccaagg tattttttcg atacattttc
    30481 tgaaaaccca cggggaaagc taatctcgtt tcccaaatag taccgtttca ggttttggtt
    30541 ttgccatgca gctgagaatt tcaagccttg gttcgccagg aatccgggta ggaatatttt
    30601 aaggtttgcc gcctgcaacg acccaaggga gtttttatta aagggtgcgc ttacaaggtt
    30661 aagcctgaaa accagaccca tacggggttg taggtcacga tgtgccatgt tccgtagcct
    30721 atgaaagtag aacccgctgt tgaccgtgcc aataccgcta tggtagctcg agtcgttgga
    30781 ggaaaaaaga taatcattcg acagggttag gtcagaaaag ggttgcaggt aggtttggta
    30841 gctgctgttg gccagctgta agggtaggta aacgtttagc tttgcctccc atctattttt
    30901 attgggaaaa acaggatttt ctgtaactct ataaagaaac gcatcctcgt cgtataggtt
    30961 aaacctcacc gacaaaaccg gccaaaatcc atagtaggtt acatcggtat gaaaaaggtg
    31021 agaatttccg tatccatagc caatactgga agtaagggtt ccggtaaggt tttgagaaag
    31081 tagcattact cccggattaa ttgaggtaac ctcagtcccg tctatgttga caaaaaaagg
    31141 catccagctg tgcacgttaa agagtgttcc cgcccccttg tagggttttg agctaaatac
    31201 tgaatcacta atttgaaggg tatcaaccac aacagaaccc ttaccaatta atgtaaaatt
    31261 tggatagcta ttatttaccc tattgcttat caaatcattt ttctgattat ctaaatcaat
    31321 agttgatgtt cctatcgtat aaccatcggg agtatagtca gaaaaaataa gctcatggtt
    31381 tttagtaacg gaacaatacc tagaactata tcttgaatta taaattttta ctgtgctgtc
    31441 tgttaagtta ttatgcagat atatattttc tacatcgcca tccgtagagc taaaaatcac
    31501 cctgctgtca aaggcgtata tcgagttaac atcatcaaaa gtaggtccgt aaacaacatt
    31561 gaatgatttg tttatatcta cggaaacaat ttccttgccc tgctgggtaa ctacaagtgc
    31621 atatagcatt cgtgtatttc tatcgaacga aagctcctga acttcgtatc cacttgaaaa
    31681 actcagcaat tctcgctcct cacgtttttt tccaatggaa accagcttaa agttaccatc
    31741 aggactgtag gaaatgcata gaactgaaca gtcgtctggg ttaatggctg gcgaaaagta
    31801 tttgcctttc ccacttagtt caattttttg ggcgtccttt atccttaaac cataaatcct
    31861 gccaaagtct tcgtactccc atcgggtatg tggctgatat tcagcccaaa caataaggct
    31921 gtccgaatag gttgggcgac caaggagata cccgggatag catagctcct taaccttttg
    31981 gctgttcagg tttatttcaa caaacgttgg tatgtcgttt agcgaggttc ggtaggccag
    32041 catggtggag tcattaagct tgtaggggta gaggtagctg gtatatgact taggcattct
    32101 aacaggatgg ttaacggctg aaagataccg gtcaaccgta tcggccttcc atctaccaat
    32161 cagatatgca aaggtgctat cggccagctt cctagccgga agtcctgtat tttgcttcac
    32221 accaaaataa aatggaatag ctgtaaaagg atacttggcc acatggttta caacctttgg
    32281 ccaagtatta atcccatact taaaattacc aaaactaacc agctgataac ctaagctata
    32341 gttattggga atataatttt tataggatcc caacagccac ttgttatatg agtaggtctt
    32401 tctatcggcc aaaaagtgag cacggtaatg ctgccagaat gttgccgacc gaccccttcc
    32461 agagaaggag tgggcagttt ccgttactac ggcatcaccc tcaagaaacc attggggaac
    32521 tagtccagcc gctaagccga ctacctgctc cccaaaaaga tagtatgcca gtcgggatgt
    32581 gctggagttg agcgcataca tttgcttaac gtggcgcatc tcgtgtaaag caagttggct
    32641 actccagggt tgagcgtaac catcggtaga tggaaaagta ataagctcca tcctacgtgg
    32701 agcccatgcc acaaaaccgt tcgatagcat ggagttggga tgaagaacca catcaatcct
    32761 ttttgacttc aggttatagt ccctaccggt aaggctatct gtagttagta ataagtttaa
    32821 gtaactagag gctttatcct catattctga aggatatatc accctaattt ttcctgattc
    32881 gatttgccgc cattttgtgt aacctggctc aactcctgtt tggtagtact gcgctttagc
    32941 cgctgtttga atcaaaaata tggttgacaa aataataatt aatcgtctca tttataaact
    33001 tatatataat aacgtaaaag gatatttatc ggatttttag cactttaaaa aagctaacac
    33061 ttccgtccga aagtgttgcc tcggcaatat aaattcccag gcttaatcca gaaatatcca
    33121 tgtcaaccac aggttcagca ccctggtagc ttagcatttt accacccgtg atgctgtaaa
    33181 gattcaatgt tacaattgaa atgccttcag gaacatgaac atgcagcttg ttgtttgccg
    33241 gattattaaa aacaagttta ggcactaggt tggactttgt ggcggaatgt aaggagaaat
    33301 cggagtaaag gctcattttg aggggcaggt gatcggaatt gttatatagg gcactcaaca
    33361 catcggtagg aacgctatag ttagcagggt ttattaacga ttcgttaaaa cgattaccat
    33421 cctgtgcaat agtcttatag ctggttattt ttaaaccatc atttccggtt gttaagctgg
    33481 atgaggataa aataaagtcg aatctatcgt ccataccacc agttgaatgg cagccgtcgg
    33541 aggttgtatg cgtggattgg gtgtgaatat ttctatagct gtagttgtta tgccaatccc
    33601 ccaccatgct aagggggtca taaaatctga actgatttat gcctgtagga gtagtgaaat
    33661 ttacgaaagc cccctcttct gcgctgtata ggttcatatc tcccatgagt aagacgtttc
    33721 cgtttaggtt gctggaagcc aggtagctca taattgcagc cgatgcagcg gtgcgatcgt
    33781 cctcgtccga aggatcgctg cctgctttga gatgggtaat catgcagtac aggaaaatag
    33841 tatcgccctg cacaagggag gaagttttta ggtataactt gtaaacattg gtttcgcgag
    33901 gacttgtagg aataacatcc tgcgatttaa gctccacctt ctcggagttg aagtatatca
    33961 tgtttgccag aaagcttccg ctaaaattag cacgtttgta cttagttatg ccatttatgt
    34021 ttagaacatt ggttctgatt ctctccacgc tcgaaacgga tgcatttacc tcgttaacgg
    34081 ttagtatgtc gggcctagca tagccaataa tggttcgcag atattcgtcc ttgctgtcaa
    34141 cattgttgtt tgctgccgta cactcctcat attcccggtc gtagtacatc aggttgtaat
    34201 gcatgactgt aagcgtatcg gcagcagcac caaaggatag aatcaaaaat atcaaaagtt
    34261 gaaatataaa tttcatagtc aatgccttag ggttgttttc taacctgaaa ctacaacatt
    34321 atacccataa gaacggagaa ttgcggcaac cttttgccgc agctcaccct gaatgataat
    34381 ttctccatcc ttaaccgatc caccaacccc acatctagat ttgagttgtt tccctaatgt
    34441 gtctaggtca tcgttgctac cccggaatcc cttaactaaa gttacgcatt ttccacctct
    34501 gtttttccgt tcaatgcata ccaccaattt ttgcttttcg ggcgaaatac tttcttgctc
    34561 cggttgcatt tcattactcg ccttaaaatc ggggttggtt gaataggcga acttcaactt
    34621 gtcattatcc tgttcctctc tcttcatttt gcctataaat agttagctcc tgattaatgc
    34681 taccttaacc agacactctg cttctggcat gttgcaaagt tatgcgcaaa gtttaataaa
    34741 tgttagtaat caaccttaaa gagctgctaa tggtaaagta aatttttgtg gttttcgttg
    34801 gtagcagatg gttagcccaa agatttcagc atttttcaaa ccattattct tctaaaaatt
    34861 ctacatttgt tgccataatt gtgttaaatt gtttgcaaaa ccaactttcc tatgggaaaa
    34921 ttcaaaaaag taaacaacat tactggttgg atagtatttg ctatttcagc agtagtgtac
    34981 cttttgacta ttgaacctac cgcaagtttt tgggactgcg gggaattcat cgccacagcc
    35041 tttaaacaag aagtgggtca tcctcctggt gcgcctcttt ttatgatgat tgcccgtttc
    35101 tttaccctgt ttgcatccga tgttacgaag gtggcagcat tggtaaatgc catgtcggca
    35161 ttagctagtg cctttacaat cctgtttctt ttttggacca tcacccatct tgcccgcaag
    35221 ctaatcatca aagatgccga aaatatttct accggaaatc ttgttgctgt tatggcaagc
    35281 ggagtagtag gtgcacttgc ctatactttt tccgacactt tctggttctc ggccgtagag
    35341 ggcgaggttt acgccatgtc atcgttcttt accgccattg ttttctgggc aatgttaaag
    35401 tgggaagagg ttgctgatga gccccattcc aaccgctggc tgatttttat tgcctacatg
    35461 atgggattat ccattggcgt tcacctgctc aacctgctca ccattccggc gctcgcattt
    35521 atttactact acaaaaaata caagcctacg cgcaacggta tttttgtggc cctactagtt
    35581 tcagctgtta tcctggtggc gatactttac attataatcc ccggaacatt taaacttggc
    35641 tccctctttg agctaatgtt tgttaatggg tttggattac cctacttcag cgggctgatt
    35701 ttctacttca tactactatt tacccttatt gcatggggaa tttacaactc ccataaaaag
    35761 aagaaagtgc tgctaaacac ggtgctcgtc tcggttgcag caatattgat tggctactcc
    35821 tcctatggca tgataatcct cagggcaaat gctaacacgc cgattaacga aaacaaccca
    35881 tccaatgttt tctccctaat taactacctt aaccgtgaac aatacggaga taggcctttg
    35941 ttttacggac aatactacaa tgcccccgtt acaggaagta aggataaaac aacacgcata
    36001 cccaagaacg gcaagtatgt tgaaacctac ttaaaaacta catacaattt tgacgaccag
    36061 ttcaaaacaa ttttccccag gatgtacagc ccacagggcg accatgttga agagtataag
    36121 aaatgggcaa atattgaagg gcgacccatt aaggtgagaa accaaaatgg ggaagaagaa
    36181 attcgaaacg ttcccacctt cggtgaaaat ctcctgttct ttttccgcta ccaagtaggg
    36241 cacatgtaca tgcgctactt tatgtggaat tttgtgggcc gtcaggacga tgttcagagc
    36301 aatggcggaa ttgagcatgg gaactggatt agcggaatta aaccaattga cgccatcttc
    36361 ctgggtaacc agaacgagct aaccgataac atgaagaacc acccctctcg caatacctac
    36421 tattttctcc ccctaattct cggcctcata ggtatttact accaactatt taccaaacgc
    36481 gataggcgca actcctttgt tgttttcctt ctgttttttc tcaccgggtt agccatagtt
    36541 gtttacttaa accaaacccc gttgcaaccc cgcgaacgtg actacgccta tgccgggtca
    36601 ttttatgctt tcacaatatg gattgggctt ggggtgctgg cagtttacga atggattcaa
    36661 aaaattgctc cctctaccgc atctgctgct gcagcaggaa tggtttgtct tagcgtgcca
    36721 attatcatgg gaactcaaaa ttgggacgac cacgaccgtt ctggacgtta caccgcaagg
    36781 gacttcgctg caaactatct caattcttgc gcaccaaacg ccattctatt caccaatggc
    36841 gataatgaca catttcccct ttggtatgcg caggaagtag agggaatacg aacggatgta
    36901 cgggtggtta acctcagcct gcttggaacc gactggtata ttgaccaaat gaagtgccaa
    36961 tgttacgact ccaaaccact gccaatttcg atgaaacatg aacagtatgt tcagggaacc
    37021 cgcgatattg tatacatcta taaccgtctg aacgatacgg ttgaacttaa aaacctgtta
    37081 aactttgtgc tgagcgacga taagtccact aagctccaaa tccctggcga atcgccaatt
    37141 gactatattc cagccagtag ggttaaagtt tctgtaaata aggctgcggt cattaaaaac
    37201 ggcactgtaa acctgaagga ttcttcgctt attgtgtcgt ccatggcatg gtctcttaaa
    37261 ggtcagtacc ttcagaaaag cgcgctggcc attctggata tcattaccaa caataactgg
    37321 gagcgtccga tttacttcgt ttcgccttac ggcgatgggg atataggaat tgccgattac
    37381 cttcagcttg agggttttgc ctacaggctg gttcctatta aaacaagcgg tagggatttt
    37441 ctttctgtag gaagaataga taccgatatt ctctacaaca acctgatgaa caagttttcg
    37501 tggggaagaa tgaacgaacc tgatgttttc attgattaca acaaccaacg tacggcaatc
    37561 gttctaaagc tccgcaacac attcagcagg cttgctcaac agctaatttc cgaggggaaa
    37621 aaggactctg ctctggttgt tcttgataag ataaccaagc ttatgcctca cgaaaaatat
    37681 ccctacgact tcttcatgtt cgacattgcc gaagcatact acaagctcaa ccaaaccgaa
    37741 acagccaaca agatattgga caagtacgcc gatgttaccc ttcaatcgct tcgctaccag
    37801 ttaagcctgg gcgataagta cagggattac aacaattacg atctccgtct taacattcaa
    37861 accctgcagg aacttgcttc ccttgccgat agctacgggc aggttgagct gaagaccaag
    37921 atagaaaacg gtttaaatca gcactatagt aagcttgtta atgtcaaata aatagtacgc
    37981 tgaaataaaa aatcatcgtt ttggtttaat caattatttt tgttgatacc tttgagcttt
    38041 aagaaaaatt catcatatgg cagaactagc tggtgaaatt gttcagatta ttgggcctgt
    38101 tgttgatgtt tcatttgaaa acagcaacgg gaagttgcct tcaatcaatg aagcgttaga
    38161 attaaggaga accgatcatt ctgttttagt tcttgaatgc caacagcata taggagaaaa
    38221 caccatccgt gctgttgcca tggactccac cgatggactt tcacgtggaa tgaaggttaa
    38281 agcaaccgga aagtctataa caatgcccgt tggtgaacag gttaggggtc gtttacttaa
    38341 tgttattggg gattccattg atggcctttc aacgttgaac cgtgaggcag actaccaaat
    38401 ccacagtgct ccacctaaat ttgatgagct ccgtccagaa aaagaagtcc tttttactgg
    38461 aattaaggta attgacctac ttgaacccta ctctaagggt ggtaagattg gactatttgg
    38521 tggtgcaggt gtaggaaaaa ccgtcatcat catggagctt attaacaata ttgccaaagg
    38581 gtatagcgga atgtctgttt ttgctggcgt tggagagcgc acccgcgaag gaaatgacct
    38641 cttgcgcgaa atgattgaat ccgatgtaat acgttacggc gaggaattta aacaaaccat
    38701 gaaggagggt ggatgggacc tcacaaaggt tgacaccgat gagctaagaa aatcacaagc
    38761 taccctcatt tttggtcaaa tgaacgaacc acccggtgct cgtgcaaccg ttgctctttc
    38821 aggccttact gttgctgaat cgttccgtga tgggggaagt ggtgaaaaag gtaacgatat
    38881 tctcttcttt attgataata tcttccgttt tacccaggcc ggatctgaag tttctgcctt
    38941 gctggggcgt atgccctcgg ctgtaggcta ccaaccaaca ctatcaaccg agatggggtt
    39001 aatgcaggaa agaattacct caacaaaacg aggttctatc acctcggtgc aggctatata
    39061 tgttcctgca gacgacctta ccgaccctgc tcctgcgact acctttgctc accttgatgc
    39121 tactaccgtt ttagaccgca aaatagccga gctgggaatt taccccgccg tgaaccctct
    39181 tgactcaacc tcgagaatac ttacccccga tgttgtggga gaagaacact acaacactgc
    39241 gcaacgggta aaaaatcttc ttcagcgcta tcaggagcta caggatatta ttgcaatctt
    39301 gggtatggat gagctgtcgg aggaggataa acttattgtg caccgagccc gtagggttca
    39361 aaggttcctc tcccaaccat ttcatgttgc cgaagctttt acaggaacaa agggcgttat
    39421 ggtaaatatt aacgacacca tcaagggatt taacatgatt atggatggtg aggttgataa
    39481 gtaccctgaa gcggctttta accttgttgg taccattgaa gaggctattg agaaaggaga
    39541 gcgcctgttg aaagaggcta aagcgtaaca cctttaccga ctacaataag agagtatttt
    39601 agacctgaac taatatagaa gggcaaatgg tttgcgactt gatttcaccc gagaggaaca
    39661 tcaaggttga aaatgtgaac tacgtcaagg tacctgggac aaagggttcg tttggcattc
    39721 taaaaaagca tgctccaatt gtttcatcac tggaacccgg agctgttaaa gttatccttg
    39781 acgataaaac taaacttctt tttgcaatac cttcaggtgg ttttgttgaa gtaactgacg
    39841 atcgggttac aatcattgcc gaagaggtgc tgcagcaacc cgaatagcct ttcttaagca
    39901 cctgttgctg gcttgcccta ctttgcaata taaggagttg tttctgtatt tttgcttttg
    39961 ccccctctga cctactattt aataaagtta ggctttaaac tggtagttta cgctgaaagg
    40021 tagaaaaaga ggcacaatta atcaacaatt gctatctgat aatagtagct ttttcgctcc
    40081 acctgaaggt gaataaattt ccatcatttt ggtttaagtg ttcaactctg caggcatact
    40141 atcattttag atacttacca aatttatctg gttactctaa cgttaatatt ttcgccttat
    40201 acccacaatt attccttaca gccaagcaaa cctaaaggca tgaaaaagat tttactacca
    40261 ttcccgatca tcatactggt aatattaacg tcaaccctta tggcaaaagc acaacaaact
    40321 gtcgtttttc aagccagcga tggactagag gttactgccg acctttacgt caagggtacc
    40381 gacttgccct atatcatctt gctccaccag gcaaggtaca gcaggggtga ataccgagaa
    40441 attgctccaa aactggttaa cttgggctac aactgcctgg cggttgacct tcgttcaggt
    40501 ggagaggtaa acggggtcgt aaataggaca gccatgctgg ctgctggtaa acgaatacct
    40561 gccgaatatt ataatgccat gagcgatgta gaaggggcaa ttggctacgt taagtcaaag
    40621 tccgacaagc ctatcgtgct ttggggaagt tcatactcag cctcgctagc attgatagcc
    40681 ggtgcaaaga acttaagggt tgcagctatt gtggcttttt caccgggtga gtattttgac
    40741 cagcctgatt ttgttagtag caagatcggc aaactcgcta ttcctgtttt agctctttcg
    40801 tctaaagccg aatgccaacc agtcagagag ctgctctcag gcgtaaatcc aaaacttgtt
    40861 tcttattatt gccctaccga ttcgggacag catgggtcta aggctctttg gcaatcaaac
    40921 ccctccaacg gtgattactg gatggctatc accatgttct tcagccggct aaacaagaat
    40981 taattcttgt ttaccggata aaagtcagaa tggaaaagcc ataccggcag agatgctttt
    41041 gaagctatcg ttcggccacc acgcacactc ttttttcccg tttaacttca cctgataggt
    41101 tgaagtactc aacatacaca acatatattc ctgttggaac aattctgttt gcatagttta
    41161 cgccatccca tctaaggcat ccctccctcc ctaaaagaag gttatttgcc aggcgaaaaa
    41221 cttctactcc ggcagaggtg aatatgcgga tgtttgaaac gtatccctca gccggcagac
    41281 tatagcaaag ctgaagaaaa tcgtccttcc catccccatc tggcgagata atttccggcg
    41341 aaatggtaaa atagccatct tccattgaag tttcagcata ctgcgaattt ttaaatgtag
    41401 gtgtggcaaa tcctacgctt tgtgctgccg aatgccaagt acttgcatcg gcagagggca
    41461 acttcgggtt aatccgttca agagaaaccc ctttgacgtt ggttataatt gggttatgca
    41521 atttttcggt atagtgcagc tcgtcaacca catccatgtt agcattgagc aacaccacat
    41581 agccctcatc actattcagc gaagccatag atgaaacctg cacaaaggcc ttttcattct
    41641 cacaatagta gaactgctta acctgttgag ggttaacagt tataaccgca tattcagcag
    41701 gaaaaatcag cctggaggta tcggctgcgg gatttacccc atttagcgat aaatctgtat
    41761 tcctgttagc taaaaatagg ttttttaaat cgatgcactt ttgagaatta ttgaaaattt
    41821 caacaaagtc aactcctccg ctatagggat taaacagaac ctcgtttacc acaaggtctc
    41881 ctgcggccgg aatttgaggt aatccaattt taaaagtact ttcttcaata gggtttcccg
    41941 aaaaatcaac aatttcacct ccaatagcta actggtaaaa gctgcctggc tccatggggt
    42001 tgtttagaat aagcttaaca ccatcgtaaa gatttccttg cagcataatc tttttcacgc
    42061 ctaagttttc agaacttaac gaatagtttt caatatcggt aatggtaagg ctatccatcg
    42121 gctcgctaaa ggcaattcta attgtgtcct ccgaaagcac ttccagatag ataacctttg
    42181 gaggagtaat atcgggatta atggccacta cggagttttc cttgcagggt gtcccaccta
    42241 gttcatccat gcaggcatgc caatttcttt tgccttcggc aatgttagag gcatcgatgc
    42301 actcgagcga gtagccccca tcactcctac tatcatcatt ataccagtca ctgctatagc
    42361 ttgcaaaaga aatcaggttg ccttcgccat caataagttt tatcagcata ccatcgttga
    42421 gcagcgaggg aaaagaggtt acacccatag ttttgccaaa tactgccatt tcactcatgg
    42481 ctgttgaccc accaatcaag gtaaaagttc ggggtaaaac agtatccgag ggaaggttta
    42541 gctggaggtc attaaggaca attcgccacc ccttggtgaa aactgtgtcg gctgaagggt
    42601 tgtagagctc aatgtactcg tactctggta atccattcga gggaattggg ttagccatga
    42661 tttcgttgat aaccaagcgt ccaaaattcc cattcgagcc taaaccaaac gttgcgctat
    42721 cggaagtaaa atggccttgt tcatcctcga tgttgcttat ataaagcgta tagctaccaa
    42781 aggacaattt ttcggttgta agtactgcca tgtcgtgttt cgactgatca atagacaccg
    42841 attttacagc aaccggttcc ccttcggcag ttctaaccag gtagttttga gggattgaag
    42901 cgctggtaat ggatacattt ttactaaaag aaaccttcac cgagtttagg ctggatagcc
    42961 ttacagattt cactaaaggg ggaaactccg ttaggcaaag ttttagatta tccaaccaca
    43021 gctgtcctgc ccttgtgggt gtatatgcat aaacaaatcc cgcatatttc attggaatag
    43081 cttcctggat aagcacttct ccaccattta ccattctacc ataatccttg ggtttgtacc
    43141 aaagcgaaag gattccggta ggcgaaaggg ttacacgaat ttcaacctca tctttttcgc
    43201 tccagtcaaa tcgtgttgta atcaggtttg ttttctttcc cccttcgaaa tgccataatg
    43261 atagaaaatc gtcaacttcc gaagggttga tgccaacagc gtaaccagat gttgtggcgt
    43321 tgttaaacaa actatcggtt gatcccagta ccatgtaaaa gtagttatcg gttgaagggt
    43381 cccagctgcc tgtttttacc acaacattcc actcaaccac tctgccataa gcattggcat
    43441 tcagaggaat aaccaactct gatttttctg caccaggatc acccgcatgt accagtgaac
    43501 tgccgccagc taccggcgaa atggttgaaa cccgccagct ttcaacaccg ccccaaccgg
    43561 gtatatcacc ctcaaaagcc tcttcaataa ggcacatttg tgaataccca caaaaaattt
    43621 ggggcgttac ggcataaagc aatgtatcgt gagatgcttt aagggtaaat cttccttcat
    43681 ttagaaattt tatgttgctc caagtagcaa ttccatcgct taaaggttct gttgaatcgg
    43741 ggatttcaat gttgccactt gcgctatttt cggatagggt tacttgaccc agctggttgg
    43801 cgtccggatt accaaaacaa tcggttgata taacttccac atcaaaacag tcgtttgggg
    43861 tagcagcaaa tggatagctg ttaaaagcaa gcctgtcggc ttcgacctct atgctacagg
    43921 gaagcccata aatatacgac aggaaattgg gacaaaatgc cgttccatca tcgtaagcta
    43981 cccacccgtg tgaggatgac ggaatataaa attggaattt ctcgttatcc tttaaaaagg
    44041 cgggattaag gtacagctct actcccattt cgatagtgtc gccatcagca atttcgagct
    44101 ggttttcgct gagctgaatg taaaaaactc cttcagattg gctgaattct attggaataa
    44161 tcaccttttc ttcaagtctc agcatgaaac caccaataat tttttctaaa gatgaagcgc
    44221 tatttgggtt aaatgcagta aggcaaattg tttttacttt taggggtaac ccatctgaag
    44281 taccagaatc ggaaactttt aggcgaaata tttcaagggc agcgctggta ttggtatttt
    44341 gtgctgtaaa tattaacgtg tcgctaccgg taaaaagcgg ataaacctgt ccgtcggcgt
    44401 cgccacaagt tatgaagggt gatgccacgt cctcaagtgt tggctccatg gcaagcagcg
    44461 caaagctacc aggtttggag tagctggcta taaagttggc tatcccgttt tgaaggctgc
    44521 cctcagtacc tccactaaca tcgagatggc cgtctcctgc tgctagacct agacctaagc
    44581 taccaaaata atcggtatca gtattgccat ttatatcagc agcctttaca accacaggaa
    44641 aagcctcgtt aacaccaact cttgctggag tttggtcaaa ttttaggtgg gtagcatgga
    44701 ctcttatggt aaaggtgctc gagtatattc cagagggaaa agtagaagaa aactgtgtac
    44761 cgttgtcgta tgcagttaaa agcggattta atgggtctat atacagcgtg attgtttggt
    44821 tatcggtaat gccggttggt tttagatata cccaaatgcc gatttccaga tttcccccct
    44881 ccgggacgca tacagagcca ttagaaaaat agatgtcaat ggttgatgtt ttgacctgaa
    44941 catccgaaag cccacaccaa gtagaaccag actttactaa gattccacct aacaccttgt
    45001 ttaaagcagc catgtttgga aggttggcac gtgttagcct taacgatttg agtaaggtgg
    45061 aagcgctatc acctcccacg tcgaatatct ttatacgcat aacttcaaca gcatcggcgg
    45121 cggaattctt agtggacaag atgtcttctg cttcgggttg aaaagaagga tttccagcat
    45181 atgaggttgt atcaaatgaa ataaaaattt cactgcttaa cgccgggctc aaaccctggc
    45241 ttgttgcctc aatccttata ttctcgtttt tgttataaat caaatcgttc caaacagcga
    45301 tacctcctat tgcaggttgc accaaaccca gcaatgaaga aagctgtcca cttccctgaa
    45361 caaggttaag ggcaatatcg tggttaaaat tcgtatccac atttcccagt gaatcgcatc
    45421 cggcaacccc gagtgcgaat ggcttgccaa taatataatt atgggaatat tcgtggattt
    45481 tcaacatggt agcatccacc ctaatggtga atagaccgga tgtaatcaag tggggtaaaa
    45541 ccttcggaaa tcctgaacca gcaacacttg ccagaaattc gtggtcaatg ctgtcaattt
    45601 taaactgtaa gctggtgcca tcggttatcc cagaggattt taaaaaaatg ctaagtgtgt
    45661 actctccggc tagcccgtcg ggaatgatca tttcagatga atctacttct agcgttagct
    45721 tggtatttga aataaacgac gacttaattg gaatttcacc gttagctcct tttagcagga
    45781 ctcctgctat tatgctgttc cattgtgaaa tttccggaat aggattggta aaggctacac
    45841 gctttacata cgtgggcaat ccatcacctg aacccatgtc ctccacaaca aacctcagta
    45901 catcaacagc cttggatggt gtggttgagg ttgataaaat ttctccagac ggtatttgca
    45961 gcattggctg ataaatggtg gtggtggtat cattaggatt tgctgcctga tagtcaacgc
    46021 cgatattgtc aacccaaagc ttcaaatcgg cagagctggt gtacctgtac tggattgaca
    46081 aatatttaaa tgcggcatac gtaacatcta cagcgctacc gtaatcgtta agattgtcga
    46141 aggttccgcc cgccgaggct ttaaggttaa atgttccgtc ggaaagtctt tttacctcga
    46201 tagccccaac atttgctgtt cctattgttg tttcccaatt taaggtagat gacaacagcg
    46261 cctggggaat gccgttatca accctccaca cctttagcat gtcatcactc cccgataggt
    46321 taacaccaac agcatagcct gaagcaggag tgcctgttgc aatttcatct cccgtcatca
    46381 ggtaaatcca ccagctgtta gaggaggagg gattataacc atgccgcaca agcatcctcc
    46441 acgttacatc tccattgtta acattccaac ttggaagatt gaccatgatg gtatcagtgg
    46501 acgagccggt attgtcaaat gtatgcttta aggaaaattc tcctgataac ggatttatgg
    46561 tagaagcctg ccatcgtcca gctggttttt gtatccaacc cgaaatatca gcattctcaa
    46621 agtcatagaa aatttgacca ctaacctcat aaaaaatcaa caggctaaaa attgatacca
    46681 ccaaccgctt cattgtacta tttagctatt tttatgcgga ttaatttacg aaaaaaacag
    46741 cagtaattca atcagtatta aaaatcacac ctatgaaggt agcagtagtg ggcgcaaccg
    46801 gactggttgg tcaggttatg cttagagttt tggaacaaag aaattttcct gtaacagagt
    46861 taattcccgt agcatccgaa aaatctgtag gtaaaacttt aaagtttaaa aatgaagcct
    46921 taaaggtaag ttcattggaa gatgctgtaa ggcaaaagcc ctccttggcg ctcttttctg
    46981 ccggctcagc ggtttcgggt gaatgggcgc caaggtttgc cgaggaagga acaacagtaa
    47041 ttgacaactc ctcatgctgg cgtatgcaca atgaaattcc acttgttgtt cccgaggtta
    47101 acgcttcagt gctgagctct tcatctaaaa ttattgccaa cccaaactgt tccaccattc
    47161 agctggtagt ggcggtgaac ccgcttcatc aacattttag agtaaaacga atagttgtgt
    47221 caacctacca atctgttact ggaactggtg ttaaagctgt taaccagctc tttgctgagc
    47281 gacagggcat tgctacagac atggcctacc cgcatcccat cgacctgaac tgcattccac
    47341 acggaggatc ttttcttgat ggaggctaca ccactgagga gcagaagttg atagatgaaa
    47401 cacgaaaaat tattggagat cctcatataa tggtttctcc tacagttgtt cgcgttcctg
    47461 tagtaggagg acattccgaa tcggttaata ttgagtttga aaaagatttt ttgcttgaac
    47521 aggtgtatga cattcttaaa aattcaccgg gggtaaccgt tcaggatgtc ccatcggaga
    47581 atacctatcc tatgcctcga atggcccagg gcagggatga tgtttttgtt ggcagaattc
    47641 gcagggatgt atcaaaaccc cattgtttga acctatgggt ggtttcagat aacctacgta
    47701 agggggcagc aacaaatgct gttcaaattg cggaatacct attgctacaa aactggttaa
    47761 aatagttctc aaaataggta ctccgcaaac cgtatcaata tttgttctgt accaataggt
    47821 taatacaaaa aattaggttg gttgcctttt ttttccgaat gccttgtacc ctaagcgcaa
    47881 cacaaatgca gcccccagta ttatcatggc ccagctaagc tccttatagt acctgtcaac
    47941 caaatcggcg gggatatagt aaccaagaag ggcaagtaca atgttccaga gtccggcacc
    48001 cagagttgtg taaaggatga actttccaaa atgcattctg gcaagccctg caggaatgga
    48061 gataagctgt cgaatcccgg gaactaaccg tccaataaac gtagagctgt tgccatgctt
    48121 aacaaaatat gattcagcct tttgaataga tttaggttca atcatcaaca agtgggcaaa
    48181 cttggtttca gcgaacgcat agataagctt acgtcctatc cagatagata taaaatagtt
    48241 aaaaatagcg ccaataattg ccccaaatgt tgaggataaa ataaccaggt atatattaag
    48301 ctctccctgg gccgccttga aggccgcagg aggtacaact atctcggaag gaaagggaat
    48361 aaaagaactt tcaatagcca taagtatggt aattgtggta tagtttatat tccccatata
    48421 ccaagctgta attctagcaa caagttctgc catagttttt ttttgtgaat tagcttacat
    48481 ttctagtgca atgttcaact tttcggcccg tcaaaggtaa tatccccagg atataaaaag
    48541 ttatcgaagg ggtatcgtcc aacatgaatg ttccttaccg aactgtatag aagcttttta
    48601 aattgatcga ggttggtttt caaccttgct gatatgaaaa ttgtctgaac attggctttg
    48661 gccatccagc tgttctgaag gctggaaaga ctagtattaa atttatcagg aggtagtaaa
    48721 tcgtcttcac ccttctcgtt ccagctatac gcatcaattt tgttaaatac cagaatggtt
    48781 ggtttgtcgc ttgccccaat ttctaccaat gtttggttaa ccacattaat ttgttcctca
    48841 aagcccggat gggagatgtc tataacgtgt agcagcaaat cggcctccct cacctcatcg
    48901 agagttgact taaacgcctc aaccagctga tggggtagtt tccgtataaa acctaccgta
    48961 tcggacaaca agaatgggag atttccgata acaaccttac gaacagtagt atcaagcgtg
    49021 gcgaagagct tattttcagc aaaaacatcg gatttgctga taaggttcat gattgttgat
    49081 ttgcccacgt tggtatatcc aacaagcgca acacgcacca actctcccct gttgctccgt
    49141 tgaatggcca tctgtttatc aatccggccc agctcttcct taagacgagc aatacgattg
    49201 cgaataacac gcctatcggt ttcaatttca gtttcccccg gcccccttag cccaataccg
    49261 ccacgctgcc gctcgaggtg ggtccaacgc cctgccaatc taggtaatag gtattgatac
    49321 tgtgcaagtt caacctgggt tttcgcatag gctgttttag cccgtcgtgc aaaaacatca
    49381 agtataagat tcgttctatc aagaacctta ataccaagtt ctgcctccag gttcctgagc
    49441 tgtgttgggg ataactcgtc atcaaaaatc accatatcca cttcgttatc cgaaatgaat
    49501 gcgagcactt caataacttt tcccttacca atgtacgtgc tgggatttgg tgattcgatg
    49561 cgttgaataa actgttttat ggacaacgca ccagctgttt cagctaaaaa atccaactca
    49621 accaagtttt cctttatctc tgtctcctcc tgcccctgct taattacacc tacaagaatg
    49681 gccttctccc tttttttctc agttgaaaaa attttcccca tat
//
LOCUS       DAOSHM010000014        49146 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_543_length_49146_cov_21.157279, whole genome shotgun sequence.
ACCESSION   DAOSHM010000014 DAOSHM010000000
VERSION     DAOSHM010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 49146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 49146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49146
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_543_length_49146_cov_21.157279"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(56..1036)
                     /locus_tag="PK839_03705"
     CDS             complement(56..1036)
                     /locus_tag="PK839_03705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG71875.1"
                     /translation="MKSHRRISMIIFLSTIVATFTVNAQENVVEVLSGNLNDAEKLAA
                     AYLEPFGKSLGSSLNNGWFNSAKPHGLFGFDITFTGAVTFPPSSAKNFDVAKLNLEYW
                     ELKNPSQNTSPTVSGEKVSGPILTDKATGLSELTLPKGSNMQFLPAPVIQVGLGLPFH
                     TEVVGRFFPKVDFPEVGNFSSWGIGVKNEFKEFIPGFKLIPIDVSVFFGYTEFTSTFD
                     IDKSKDQTLNFNASGFTGKLLVSKSIPVLTVYAGVGYVKSTTDVKLKGYYDIPNVGLN
                     MKDPLALDFSNNGFNANIGIKIKLALITFHCDYSFGDYSVLNTGIGISFR"
     gene            1237..2517
                     /locus_tag="PK839_03710"
     CDS             1237..2517
                     /locus_tag="PK839_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005641312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HOG71876.1"
                     /translation="MSSIPLAERMRPHTLDDYVGQSHLVGEGSVLRDMVKSGNLNSFI
                     LWGPPGVGKTTLAKIVANELKRPFHILSAINSGVKEVREVIEKAKSQKFFNSPSPILF
                     IDEIHRFSKSQQDSLLGAVEQGVITLIGATTENPSFEVISPLLSRCHVYILKPLSNEE
                     LSLLLNRAISTDVELKKIKFKIAETEALYHFAGGDARKLYNIIELLASVAGGKGEVEI
                     SNEKVTKVLQENIALYDKNGEQHYDIISAFIKSVRGSDPNAAVYWLARMLEGGEDPKF
                     IARRLVILASEDIGIANPNALLLATSCFQAVHLIGMPESRIILSEASIYLATSAKSNS
                     AYEAIDKAQALVRTSGNLPVPLHIRNAPTKLMKEIGYGADYKYAHSFKGNFVDQEFLP
                     SGISGTTLYEPQDNPKEREIAMRLRNLWKDKYNY"
     gene            2554..3360
                     /gene="kdsA"
                     /locus_tag="PK839_03715"
     CDS             2554..3360
                     /gene="kdsA"
                     /locus_tag="PK839_03715"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HOG71877.1"
                     /translation="MTSIKHLDSGNFFLMAGPCVVESEELAITVAEKVSAIAEKFKIP
                     YIFKASYRKANRSRIDSFSGIGDLKALKILQKIRTQLQLPVVTDIHSAEEAGIAAEYV
                     DVLQIPAFLCRQTDLLIAAAKTGRVVNIKKGQFVSPESMKYAAQKVVDSGNSKVILTD
                     RGTQFGYQDLIVDFRGIPQMRAFGFPVVVDVTHSLQQPNQTSGVTGGRPDLIETIARA
                     AVATGADGIFMETHPNPAVALSDGANMLDLKYMEELMNHLVQIRQVIRNF"
     gene            complement(3347..4765)
                     /locus_tag="PK839_03720"
     CDS             complement(3347..4765)
                     /locus_tag="PK839_03720"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG71878.1"
                     /translation="MTILNVILGITCILLVGYVVLIHRRIHPRNITGSSDQLKDIEAL
                     KIENAELKIRCRELQVDNDELNSLLSTQALEQSRLREALERTKRKAEESDILKSNFLA
                     NMSHEIRTPMNGILGFAQLLKNEESAEKRDRYIDIIYHNGLMLVNLIDDIIDISKIDA
                     GQLAITKAECDLDNLLFEVYTFFNEVKFKQEKEHINIRLLNLNDGEANILYTDGLRLR
                     QVLVNLISNALKFTEKGFVEFGFVNRPEERQIEFFVRDTGIGIPTDKQDIIFERFRQI
                     QEGSTRKYGGTGIGLYISKSVVNLLGGDIWFESTCGVGTTFYFTIPYEDVNVAHSGAK
                     FYRTSSPEYSWEGRNILVAEDTESNYLYISTILAKTKANVIWARNGEEAVNAALRGAV
                     PVDLILMDIQMPVMDGYEAVTAIRRQNAEVPIIAQTAYALPHDNIRCFEVGCNDYISK
                     PINALLLKQKINELLNAYIKNS"
     gene            complement(4976..5647)
                     /locus_tag="PK839_03725"
     CDS             complement(4976..5647)
                     /locus_tag="PK839_03725"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HOG71879.1"
                     /translation="MRKLFILVLALFSFTIANAQFLRFGVKGGVSSTNVKIDETSLQG
                     LTTTDGLKQFAVEQGNSKLGLHFGVFGRIQLAGLFIQPEILFTQSKGEVVLSYINSAD
                     EAIQTVEDQKFNKIDIPVMVGWKFGPARIGLGPVASIMLSENDGLKKELASLTSQSAE
                     SNFKNAVFGYQLVAGIDILKKITFDFKYEGNLSKLGEGITLGGSDFKFDQRNPQFIFS
                     LGIFF"
     gene            complement(5723..7195)
                     /gene="cls"
                     /locus_tag="PK839_03730"
     CDS             complement(5723..7195)
                     /gene="cls"
                     /locus_tag="PK839_03730"
                     /inference="COORDINATES: protein motif:HMM:TIGR04265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HOG71880.1"
                     /translation="MILLNFFMNVAMANLNRIILIIYFITVLFTIIMVIHEKRDPSKT
                     STWVLLIISLPVLGLILYVIFGQNRRKEKIFNRKEIQDIQQIDYLSRQQVTNFSKKGQ
                     LNSKISNHISIITLLLNNSKALLTDFNSVSIFFKGKPAFDSIIDELNNATSSIHLEFY
                     IINDDKIGNRIKEVLIAKARLGVKVRVIFDDVGSWSLPKKYIRELKQAGVEVFPFMKV
                     RFPLLTSKANYRNHRKIVLIDGRVAFLGGMNIADRYIEGSKIGMWFDTMLRIEGEAVH
                     SLQVIFLIDWYFVSGDLVADRPLYFPEVSVSTYHPIQVATSGPDSDWASIMQAFFLGI
                     TKASHHVYIATPYFIPNESILTALKTAALSGIDVQIMLPGISDSTIVYWSSLSYVSEL
                     LEAGIKVYLYRHGFNHSKLMMIDGSLAFTGSANMDIRSFEDNFEVAAIMYDDQITRLL
                     ESSFIQFRNSSQEITLEEWNRRSAGNSFKEAFARLISPLF"
     gene            complement(7272..8078)
                     /locus_tag="PK839_03735"
     CDS             complement(7272..8078)
                     /locus_tag="PK839_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOG71881.1"
                     /translation="MKGKIAIITGASSGIGKACAYEFASRGAKLVLAARNLEVLNSIV
                     QDLLLNGHEAIAVRTDVTVEDDCKNLVDEALKRYGKIDYLLCNAGISMRALFKDVDLK
                     VIRQLMEVNFFGAVYCTKYALPHLLASRGSVIGVSSIAGFHGLPGRSGYSASKFALQG
                     FLETLRIENLKNGLHVMVAAPGFTATNVRVSALTADGSPQGVSPRDEKHMMTPQKVAR
                     TIAHGILRKRRNIIMTTEGKLSVLAQRVFPKIIDRILYMFMAREPNSPFK"
     gene            8370..8442
                     /locus_tag="PK839_03740"
     tRNA            8370..8442
                     /locus_tag="PK839_03740"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8403..8405,aa:Lys,seq:ttt)
     gene            complement(8660..11032)
                     /locus_tag="PK839_03745"
     CDS             complement(8660..11032)
                     /locus_tag="PK839_03745"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOG71882.1"
                     /translation="MKKLLFIISIATLAACQPSPNLDGVAEKPAVADLLSAIQLKGDS
                     TKVVLTDYFPDNKVVDSITIPDFFRYSYSPDSASITLVANNLNLPPLSIIQVWVKGNE
                     RYDLLVRKSKKLPVAFTFDPEGKKVKSVQLAGQINDWNPNATSLTFDGKVFRTTLLLN
                     PGKYHYQVVVNGKWMLDPANPLKEDNGMGGQNSVVEVGNLKPNMLPKLVALTAHKNIL
                     NIKATGTVDSIIVLWQNYLLTGNFIKRDGDVFHVTLPRNAEEYSRSYIRVWAYNEFGE
                     SNDLLIPLEGCRVVEDPGKLTRSDKHTQVMYFLMVDRFNNGTTANDRKVDDPEILPKA
                     NYFGGDLVGVTQKIKDGYFTSLGINTIWLSPITQNPLGAWGLYPNPRTKFSGYHGYWP
                     VSLTKIDFRYGTDGDLRELIDEAHSRNMNVILDYVANHIHVEHPLYKQHPDWTTSLYL
                     PDGSLNTERWDEYRLTTWFDVFLPTLDLQRPEVYEPMTDTALYWLTNFQLDGFRHDAT
                     KHIHENFWRRLTQKIKLKVTDRSIYQIGETYGSRELVASYIGSGMLDSQFDFNIYDAS
                     VAAFARSNYPFSSLNSSLLESFSYFGWNNLMGYISGNQDRGRFISYAGGALRFDEDAK
                     KAGWTRDIGVGDTSAYSKLCMLNAFNLTIPGVPTIYYGDEFGMPGGNDPDNRRMMKFD
                     NLSPIENRTLDVVKKLVNLRRNTMALNYGNFETLLADSTQYAYARTYLQSSAIVVFNK
                     SDEDKSIIFKLPDYMVGRNFTSKFGSNSSIQNQIVKVDLKPNSFEILISK"
     gene            complement(11068..12915)
                     /locus_tag="PK839_03750"
     CDS             complement(11068..12915)
                     /locus_tag="PK839_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HOG71883.1"
                     /translation="MKKPLILMLMLIAACSMNVNSQNIKRVEPPCWWVGMKNPRLQLM
                     IYGSNINQAQVGISDSRVTLESVDKTENPNYLFLNLAINENAEPGAFNITFKFPKGKP
                     RVVAYELKERKQGSSERIGFNNSDVIYLVFPDRFSNGNPANDSIKGFSDKYNRNDPYG
                     RHGGDIQGIINHLDYFNQLGVTALWLNPILENNQPSTSYHGYAITDFYKVDPRLGDNE
                     LYRKFSQMAHERGLKVVKDMVFNHCGSSHWWMNDLPMHDWINQFPQFTRTNYRIPTTF
                     DPHASTVDSSLMADGWFDLTMPDLNQRNPYMATYLIQNSIWWIEYADLNGIRMDTHPY
                     CDKHFMSRWCAAVMSEYPNFSIVGETWVNYPDWVAYWQKDAHNQDGYNSNLKVAMDFP
                     LMFAMHKAFDEKDGWDTGLARLYEILAHDFVYPSPNNLLIFADNHDIGRFHRDSSLAL
                     GRLKLAMAFLLTTRGIPEIYYGTEIILPGDDAKGHGDIRRDFPGGWEGDLKSAFTTDG
                     RTNRQNEIFNYISTILNWRKTASAVHSGKLTHFIPENEVYVYFRFDDKQTVMVILNNG
                     YQPKMLETARFSEMMKGKNSAVDIISGKVFDNLEKIQLSPRSALILELR"
     gene            complement(13052..14113)
                     /gene="fbaA"
                     /locus_tag="PK839_03755"
     CDS             complement(13052..14113)
                     /gene="fbaA"
                     /locus_tag="PK839_03755"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HOG71884.1"
                     /translation="MHFKPGIITGNELNKLFEYAKQEGFALPAVNVTGTNTMNAVMEA
                     AHEVGSPVIIQFSNSGASFISGKSISNDGQRASILGAVSGAKHVHLLAEAYGVPVVLH
                     TDHAAKKLLLWIDGLLDMGEKYYKETGAPLFSSHMLDLSEEPLHENIEICKRYLERMS
                     RMGMTLELELGVTGGEEDGVDNTGVDNSRLYTQPDDVAYAYEELMKVSPNFTIAASFG
                     NVHGVYKPGNVVLNPIILKNSQELISRKFGKGSKPVNFVFHGGSGSEKEKIAEAISYG
                     VVKMNIDTDLQWAFWDGIKNYYQSKKEYLQGQIGNPEGEDSPNKKYYDPRVWLRKGEE
                     SVINRLKEAFADLNALNRA"
     gene            complement(14144..16468)
                     /locus_tag="PK839_03760"
     CDS             complement(14144..16468)
                     /locus_tag="PK839_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 65 glycosyl hydrolase domain-containing
                     protein"
                     /protein_id="HOG71885.1"
                     /translation="MNTYLKHDEWRIIEDNFIPENTRASESIFSIGNGAMGQRANFEE
                     KYSGNSLQGSYIGGVYYPDKTRVGWWKNGYPEYFAKVINSTNWIGISVKVNGEELDLA
                     TAKVLNFCRILNMKEGYLERSFTCELPNGIKLEVKSTRFLSMVDTEIGAIRYSIKPVN
                     SNASIQISSYLDADVHNEDSNYGEKFWDVIDVRGGEIFSYIISQTKKLDFQVGHAMRN
                     LLFINGQPQNLKPQVNSGQMYVDNRFTVNASSGDEVILYKYAAVISNLNHEKGKFTKV
                     LETLIEGASEKGFDKLFDEHRKAWADIWNHSDITIEGDVAAQQGIRFNIFQLSQTYTG
                     RDERLNIGPKGFTGEKYGGSTYWDTEAYCLPFYMVTHGQKVARQLLLYRYKQLGKAIE
                     NAHKLGYTDGAALYPMVTMNGEECHNEWEITFEEIHRNGAIAFAIYNYVRYTGDKDYL
                     VDFGLEVLIAIARFWSQRVNFSTEKGKYVMLGVTGPNEYENNVNNNWYTSTIACWCME
                     YAEEVSSYVKSTNNAKYQELCSRIGFDEVNEIARWKEIRSNIFLPEDKKLGIFLQQEG
                     YLDKEQILVKNLDPKERPINQKWSWDRILRSCFIKQADVLQGLYFLEDRFDTETIKRN
                     YEFYENRTVHESSLSPCVHSVLASRIGNVEKAYELYLRTARLDLDDYNNEVKEGLHIT
                     SMAGTWISIVEGFGGMRVRSNMLTFNPLIPQNWKSFSFKVWFRGNVLKIRVTNSELSL
                     FNEVGSNITVKIYGKDQLIPEGGAITIAVQKQVY"
     gene            complement(16485..17147)
                     /gene="pgmB"
                     /locus_tag="PK839_03765"
     CDS             complement(16485..17147)
                     /gene="pgmB"
                     /locus_tag="PK839_03765"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019150661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0046352 - disaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HOG71886.1"
                     /translation="MSSIKGCIFDLDGVVVDTAKYHYQAWKRLANELGFEFDESHNER
                     LKGVSRMRSLEILLEVGGLSFPQEKMEQLAARKNEWYVEYISRMTPNEILPGSKELLE
                     ELRAKGIKLALGSASKNTPLIMDRIGLKDHFDAIIDGNIISIAKPAPDIFLAASKAIG
                     VEPPCCIVFEDAEAGVEAAINAGMRVVGIGNPQVLKRANLVVPNLKNFTFQYLVNSLS
                     EK"
     gene            complement(17140..18489)
                     /locus_tag="PK839_03770"
     CDS             complement(17140..18489)
                     /locus_tag="PK839_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOG71887.1"
                     /translation="MAKKPKLRFWQIWNLSFGFLGVQAGFALQNANVSRILSNYGADL
                     HHLSFFWLLAPIMGLIIQPWVGAASDKTWNRMGRRKPFIFGGAIVATLAMLFMPNSQW
                     AVLIIPPVAFGAIMLALMDASFNVTFQPFRSLVADMTPAEQTNLGYSVQTLLINLGAV
                     IGSFLPYFLTEVLGIGNTAPEGHVPPSVIWSFYIGGALLLVSVLWTVFTTKEYPPKEF
                     AEYSGQTLEETPTKKGKSNFFKTLLSMPNIMHRLAVVQFFSWFALFTMWVYTTPAIAQ
                     HIWGTEIGDSTSFAYNEAANWVGVLFGLYSVFAALFAIFLNKIADKLGRIKTYSISLL
                     LGGLGFLSIYLFNNQYALIISMLGIGIAWAAILAMPYAILANSLPAERMGVYMGIFNI
                     TVVVPQIVSGIVLGTMLKSFFNDEAIYMILLAGFSMILASASAMLFVKDNSKVLPNE"
     gene            complement(18935..20515)
                     /locus_tag="PK839_03775"
     CDS             complement(18935..20515)
                     /locus_tag="PK839_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HOG71888.1"
                     /translation="MNFEEEVKRRRTFAIISHPDAGKTTLTEKLLLFGGAIHTAGAVK
                     SNKIKKTATSDFMEIERQRGISVSTSVMEFEYQGYKINILDTPGHQDFAEDTYRTLTA
                     VDSVIIVIDHAKGVETQTRKLMEVCRMRKTPVIVFINKLDRPGNDPFDLLDEVEKELG
                     IGVRPLSWPIGQGPTFKGVYNLFEQKLFLFSPDFKQKVADEVVEITDINSPELDKYIK
                     PYTAKLREELEIINEVYPPLDINTYLSGRVAPVFFGSALNNFGVKELLDCFLQLAPYP
                     RPAEADIRTISPFEQKFSGFVFKIFANMDPNHRNRIAFLKVCSGKFERNVPYYHVRMA
                     KTFRFSSPNAFLADKKSIVDVAYPGDIVGLPDSGNFKIGDTLTEGEIINFKGIPSFSP
                     ELFRYIENAEPLKYKQLAKGIDQLTDEGVAQLFTQNSNGRKIIGTVGALQFDVIQYRL
                     EHEYGAKCKYEPLNIFKACWIVSSNKAQLDDFRIRKYSNLATDKQGREVYLAESPYAL
                     QMAQDKFTDIKFYFTSEF"
     gene            complement(20597..21889)
                     /locus_tag="PK839_03780"
     CDS             complement(20597..21889)
                     /locus_tag="PK839_03780"
                     /inference="COORDINATES: protein motif:HMM:NF012593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HOG71889.1"
                     /translation="MRGARRLPLYAKILIGMLLGLILGIIAIPLGANQLVSDYIKPFG
                     TIFLNLLKLVAMPLIFASLVTGIAGMTDVGKLSRIGIKTIAMYLITTLLAVTIGLSLV
                     SLIKPGSYFSDDRRQEFVSKYSLDISSRQQAAESISQDSPLQFLVDIVPENIVQAASS
                     NTAMLQVIFFALLFGIALVLVRNPKVEVVNNFIQGLNEVILKIVEIIMFFAPFGVFAL
                     MASLIVDFAGDSLSSSLQLFASLGMYALTIIIGLIFIIVIVYPLFLKIISGTGFGKFY
                     KSMFPAQLVAFSTSSSAATLPVTMKQVESELGVHEDISGFVLPLGVTINMDGTSLYQA
                     VATVFIAQVFGIDLTFSQLLTILLTATLASIGSAGVPGAGVVMLIIVLNSVGLPVEGL
                     ALILAIDRPLDMLRTVVNVTGDATIAVCIAKSEGKLEA"
     gene            complement(22019..22552)
                     /locus_tag="PK839_03785"
     CDS             complement(22019..22552)
                     /locus_tag="PK839_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG71890.1"
                     /translation="MHRNRLVKLLIGIVLVAFTSCGGSDDDESDNFIRFGNEKFRVYL
                     GYQYKYSETLQETGSTPFVVSLLGEGVKYSTDNAAFTGTGSLIRLYMYSESNVEVKNG
                     DYTIDIFSTKQVYSADSCSVYFNYNFDVDTGKVYPVKAGVFKVENLGRLMSYKLNMVN
                     ADSLHFVGEYKGVFNTL"
     gene            complement(22670..23161)
                     /locus_tag="PK839_03790"
     CDS             complement(22670..23161)
                     /locus_tag="PK839_03790"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOG71891.1"
                     /translation="MNISNDKVVSISYELKVNGDLVDSAVADNPLVFLYGHGQLLPLF
                     ETNIHNLKTGDSFEFSIPYADGYGEVDEEAIIDLPKDIFVIDGVLQEDMLVVGNTLPL
                     RDSEGNELNGTILEVGDTHVRMDFNHPLAGEDLYFTGKIIDVREATSEELSHGHVHAH
                     GHH"
     gene            23404..23604
                     /locus_tag="PK839_03795"
     CDS             23404..23604
                     /locus_tag="PK839_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HOG71892.1"
                     /translation="MKGKVKWFDAAKGFGFIQTEEGSDVFVHYTGIAKDGFKRLDEGQ
                     EVSFEITEGKRGPQATNVVVLE"
     gene            complement(23657..24289)
                     /locus_tag="PK839_03800"
     CDS             complement(23657..24289)
                     /locus_tag="PK839_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG71893.1"
                     /translation="MRKIIANLTKHSIKILSYLLVATGVMLIGSARAQDQEQGRNFDR
                     FSDRLFFGGSFGLAVGSRVTQVDVAPMMGIWIFPEWSLGLGGRYSYRRERFSINGFTT
                     EPIETHVWGVSGFTQVIPITDFHRTFGIDLHGGIILHGEFESLYLDRNFFDQSSSYGK
                     GWVNMYLVGGGWHQPIGDRAAINFLVLWDLTDNRYSPYNSNPILRFTITF"
     gene            24457..25164
                     /locus_tag="PK839_03805"
     CDS             24457..25164
                     /locus_tag="PK839_03805"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOG71894.1"
                     /translation="MNIIVTGASRGIGYQTVLQLAKSAGNRIIALARDHEQLTRLQLE
                     SRKVQPESEVIPLVFDLQQTSSYDDALIPQIKGHFTSADILINNAGLLVNRTFEQLTL
                     LEMVQMLTVNFIAPAYLTKLIIPLMTKGGHVVNISSMGGFQGSQKFKGLSIYSASKAA
                     LASLSECLAEEYINKGISFNCLALGATDTEMMRAAFPGYNAPIKPNEMAKFIAEFAIK
                     GNQYFNGKIIPVSTSTP"
     gene            complement(25374..26450)
                     /gene="buk"
                     /locus_tag="PK839_03810"
     CDS             complement(25374..26450)
                     /gene="buk"
                     /locus_tag="PK839_03810"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HOG71895.1"
                     /translation="MDEFKILAINPGSTSTKIAVYNNNKSIFLKSIKHSVEDLAQFKT
                     ITDQFDYRKKIILEELGQTGIDVNKISAVVGRGGLLKPIESGVYEVNEQMKSDLRSCI
                     MGEHASNLGALIADDIAQSLPNAKAFIADPVVVDELNDVARISGHPLFKRVSIFHALN
                     QKAIARLHAKSLNKPYEELNLIVAHLGGGISVGAHHNGRVVDVNNALDGDGPFSPERA
                     GTLPAGQLAKLCYSGQYSYDEVKKMITGKGGLVAHTGTNNAYEIEVKARGGDAMAKLL
                     QDAMAYNVGKAIGEMATVLKGKVDSIVLTGGIAHNPDLVEYIKEMVGFIAPIAVYPGE
                     DEMQALAMNALMVLKGEVIPKIYR"
     gene            complement(26478..27383)
                     /locus_tag="PK839_03815"
     CDS             complement(26478..27383)
                     /locus_tag="PK839_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HOG71896.1"
                     /translation="MRVDKLDQLFDLVKSKPRRRLVAAFANDAHTIEAVSLAVDRGIV
                     DATLVCDEPVMKKVCAEHNIDPGKFRIVQEADEMKAARRAVELINRGEGDVLMKGLVS
                     TDKYMRAILDKENGLLPPKAVLSHVTVVEVPTYPKLLIVGDVAIIPAPDLNQKIAIVN
                     YLIQTAHSLGIEKPKVALNAATEQMLAGMPACVDAAIISKMADRGQIKGAIVDGPLAL
                     DVAIDMESAQIKKLGGGVAGDADCILFPNIEAGNVFFKSCTKLANGELGAMVMGAKVP
                     CVLTSRGDSIKSKMYSIALAANAVK"
     gene            complement(27419..28630)
                     /locus_tag="PK839_03820"
     CDS             complement(27419..28630)
                     /locus_tag="PK839_03820"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HOG71897.1"
                     /translation="MIILVLNCGSSSIKYQLINMANESQLLAKGIVERVGFKDAILTH
                     KPVGKDKYEMVASIPDHTTGINLILEALVDKNHGVVESLSQIKAVGHRVAHGGEFFTK
                     SCLIDSNTKREIEKCIELAPLHNPANLKGILSMEKLLPNVPQVAVFDTSFHQTMPKEA
                     YLYALPYEYYENYKIRRYGFHGTSHMFVAKKACKILGADFDQLRIITCHLGNGASIAA
                     IKNGKSIDTSMGFTPVEGLIMGTRSGDIDPGILLFIAEKENLSIQQVNDVINKKSGVF
                     GISGISSDMRDLEDAAASGNERARLALDMYYYRVKKYIGSYAAAMDGVDLIIFTGGIG
                     ENDSTLREKVCGALDFMGVEFDVEANRGVRGQDKLLTKPNSLVKVMAITTNEELVIAT
                     DTANIINNLKN"
     gene            28913..31858
                     /locus_tag="PK839_03825"
     CDS             28913..31858
                     /locus_tag="PK839_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008664789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HOG71898.1"
                     /translation="MGIHMIEDFEKLATRLKGEIRWDTPTLLQYATDASAYREIPKAV
                     VWPKGKSDLQELVSFAQQQNLTLTPRTAGTSLAGQVVSNGVIVDLSKYWGKIIEVNQQ
                     EHYVWVEPGVILDELNLYLKPYGLFFAPETSTSNRCMIGGMVGNNSCGSHSLIYGSTR
                     DHLLSVRIMLDDGEVHEFSNLTYIEFEAKCKLSNREGEIYRFFEQLLGKEGNRKLIAE
                     NSPDPSLRRRNSGYALDHLATNTFFNGSTNPFNLAKLVAGSEGTLGIVTEIKLNLVPL
                     PPKEKAVVAVHLEKREQAFRANLIALKYYPAAVELMDNVILNCTKGNIEQQKNRFFVQ
                     GEPGAILMVEFYGSNQQEIEEVANEMENEMRLNGYGYYFPIIWGNETTKVWALRKAGL
                     GVLSNVIGDAKPVSLVEDTAVAVEKLPDYMEEFAGIMGKYNLDCVYHAHIGTGELHLR
                     PVLNLKDPNDVELFRKVGLEVASLVKKYRGSLSGEHGDGRLRGEFIPFMYGNEVYQMM
                     LQVKKTFDPNNIFNQGKIVNTPPMNTSLRYQPGIPTREIATIFDFSGTQGIIRAIEKC
                     NGSGDCRKTHRMGGTMCPSYMATLNEANTTRARANLLREYLTNSPKDNPFDHKELYEV
                     LDLCLMCKGCKSECPSSVDMTKLKAEFLQHWYDIHGIPFRNKMVAYITQIYRLGAIFP
                     KFTNLLLSSKVTSFAIKNMLGFAQSRALPKLSSITLRRWQAKQKNSNKYPNGIVYLLP
                     DEFTNFNESHIGVKAAELLNYLGYEVRIPDILESGRTYISKGLIRTAKRIANNNIDKL
                     KGIIGEDTPLVGIEPSAILSFRDEYPDLADDRNTKASKELAMNSLLFEEFFMREASKG
                     KIAKEHFKQGNLRIKLHGHCQQKAVASTQATKAMLSFPEGYEVSEIPSGCCGMAGSFG
                     FEKEHYELSMKIGELVLFPEIRKSSETDVIVAPGTSCRQQIYAGTGRYALHPIEVMWQ
                     AANISSQRS"
     gene            complement(31871..32743)
                     /gene="sucD"
                     /locus_tag="PK839_03830"
     CDS             complement(31871..32743)
                     /gene="sucD"
                     /locus_tag="PK839_03830"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HOG71899.1"
                     /translation="MSILVGKNSRVLVQGFTGSEGTFHATQMIEYGTNVVGGVTPGKG
                     GQKHLDRPVFNTVDDGVKTTGADVSIIFVPPAFAADSIIEAAAAGIKLVVCISEGIPV
                     ADMVRVKYFLEDFPATRLIGPNCPGIITPEEAKVGIMPGFIHKKGHIGVVSRSGTLTY
                     EAVDQLTKLGLGQSTGIGIGGDPIIGTTTLDAIKLFMNDPDTHGIVMIGEIGGSMETE
                     AAQWVKIHGTKPVVGFIAGQTAPKGRKMGHAGAIIGGTADTAEAKMNTMRECGIHVVE
                     SPANIGRAMKDALK"
     gene            33021..34484
                     /locus_tag="PK839_03835"
     CDS             33021..34484
                     /locus_tag="PK839_03835"
                     /inference="COORDINATES: protein motif:HMM:NF015882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HOG71900.1"
                     /translation="MAKVNIKLENLSEMFPNSWTDEQKAKGNTLFLKYLSEKAHKFYG
                     GKVQTVPKAPVPGFNWFNVWYTPGVSKVSTTIRDNNDTSFELTNRGNLVAVVSDSTRV
                     LGDGDCTPPGGLGVMEGKAFLMKYLGGVDAVALCIDSKGKDGKNDPEKIIDFVKMCQH
                     SFGAVNLEDISQPNCYRVLDVLREECIIPVWHDDAQGTACVTLAGLLNALKLANKKMS
                     NAKMVLFGAGAANTTIARLIIADGGDPHNMVIFDSHGGLHKNREDIKADHRFYRKWEL
                     CEQTNPQCINTMEEAMRNADVLISVSTPGPDTIKQEWVKLMAPKSVVFACANPVPEIY
                     PYAAKEAGAYIVATGRGDFPNQVNNSIGFPGILKGALIARASKITDSMAIAAAHSLAG
                     FAEKRGISPENIVPTMDEADVFPVEAADVAVQAVKEGVARRQVSWEEAYRIAKKDIES
                     SRSMTKALYDNGYIANPPAEMLEDALLWTIQQVEAKK"
     gene            34830..36731
                     /gene="ftsH"
                     /locus_tag="PK839_03840"
     CDS             34830..36731
                     /gene="ftsH"
                     /locus_tag="PK839_03840"
                     /inference="COORDINATES: protein motif:HMM:TIGR01241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOG71901.1"
                     /translation="MTKREKYSWVYWLSILAFVIALQLLVFPDTQGPREFTYSEFIDS
                     VNAGKVERIVFFEDKIIGQLKSGEETGQKSITQAPTAPWRLRIPSIEQQVKRQFVVNR
                     IPNMNEEYLISILTGTNVSFKGHVENNVLRNFFLNWLLPLLIFMALWGFFFKKMGSKN
                     PMLEIGKNKAQIHAEKPKNPITFKDVAGVDEAVDEVRELVDFLKYPEKFTRLGGKLPK
                     GVLLVGPPGTGKTLLAKAVAGEADVPFFSLSGSDFVEMFVGVGAARVRDLFNQAKVHA
                     PCIIFIDEIDAIGKSRAQNAAYSGGYDERENTLNQLLVEMDGFDATSGVIIIAATNRP
                     DVLDPALLRPGRFDRQVLVDKPDMKGREAIFKVHSRNLKLSSKVDLSRLAAQTPGFAG
                     AEIANVCNEAAILAVRNNRDEVTMSDFEAAIERVVAGLEKKNKLINEKERQIVAFHES
                     GHAVVGYFTPGAEEVQKVSIVPRGIGALGYTLQMPLEDRYLMSKSELLGKIKGLLGGR
                     AAEEVIFGEVSTGASNDLERASQLARSMIIVYGMSSKLPNISLVNKSNPGFLGQPSGI
                     ERRSEYVEKLIDEEVNEIINTCYHEAKMLLKEKKHLLDKMASILLKQEVINHDEIISI
                     LGDRTPIED"
     gene            complement(36755..37201)
                     /locus_tag="PK839_03845"
     CDS             complement(36755..37201)
                     /locus_tag="PK839_03845"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HOG71902.1"
                     /translation="MRDDFNEDLLNLPFNIHRTSVAIRGEITRRLSEAFGDDFTVDYW
                     YILTLLFSSEPLSQAHLAQISGRDKGSLSRTLACMERLDLVVRLPNPDNSRSELVSST
                     KAASNFKPKAESIILDVLSEALSSLRPIEVIELNRMLAAINEKIQP"
     gene            37580..39994
                     /locus_tag="PK839_03850"
     CDS             37580..39994
                     /locus_tag="PK839_03850"
                     /inference="COORDINATES: protein motif:HMM:NF023266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HOG71903.1"
                     /translation="MEYRLYFSIPNRLSSPVPGRAELNFELINRKDLVIDFMTEATNL
                     QSITVNNRLLSIRVKNGHINIPSKYLLTGQNKVNIDFVSCSPSLNRKADYLYTLLVPD
                     RASTVFPCFDQPDLKAAFNLTLEIPKEWVAVANAPLGKIIESDTSTVYVFEKTKPVST
                     YLFAFACGKFKVASQPTRYGEMAIYYRDNDSAKVNRNIEAIYELHTQSLGWMENYTAI
                     LYPFSKLDIVLIPDFQYNGMEHPGAIYYRESSILLEGDPSENKLLNRANLIAHEVSHQ
                     WFGDLVTMRWFNDVWLKEVFAGLMADKIVNPQFPEFNHQLSFLLSHYPRAYSVDRTAG
                     ANPIRQELDNLLNAGSLYGDIIYHKAPIALMQLEMAMGVSAFQEGLRKYLNTYSMGNA
                     SWDELIDILDPLTSLNLSVWSKAWIESVGMPTLKATLNDSMYVVKKIGVQLPMVYSVE
                     VLCGSNVNEAKPIQHIQPEVSTLFASSCELERVIVPNADGLGYGMFVPDSQSLKLLCD
                     SSFRIADPVTRSASLISANELFLNGKLDGNTYFNMLLASLARENEPQVRSYILASIET
                     YWWTFLADSARENLSQQVEIQFSKLLLNSSIPADERKSIFKTYARIAVSSNAAEELYG
                     IWSGNKSLPGLTFTETDLTFLAYELAVRNVHNADSILDLQEKRIANPDLLAKFRFVRR
                     ALSPDDALRDDFFHSLLIPENRRPEPWVAEALHYFNHPLRDSFASQYLRASLDLLPEI
                     QQTGDIFFPKTWLDAILWGKNSNQAAEIVSSWLNQHPNVPDNLKNKLIQSSDLLFRAV
                     DKRKKK"
     gene            complement(40077..41768)
                     /locus_tag="PK839_03855"
     CDS             complement(40077..41768)
                     /locus_tag="PK839_03855"
                     /inference="COORDINATES: protein motif:HMM:NF013591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008241 - peptidyl-dipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M2 family metallopeptidase"
                     /protein_id="HOG71904.1"
                     /translation="MNKIKPIMPVMLMIVLVAASSCTSNQKKMEKQMADFIAKVESKV
                     IPLSKEAAIASWDASISGKDEDYQRSEKAQVELVKIFADTSLFAELKSIKESGLVKDS
                     VLSRQMDILYLAFLRNQVDPTLLEKEIAMEANLEKKYSNFRAKIGSKQLTDNEIEDIL
                     ATSTQSSEVESAWKAHKKIGPEVAPDIIELVKVRNQIAQKLGYANYHTMNMVLSEQDP
                     DEVLALFDELDSLTSGSFSELKLEIDSYLSKRFGVSPDKLMPWHYQNRYFQEAPKIYN
                     VNLDQFYSDKDVVALTAKFYNGIGLNIDDIIARSDLYEKPGKNQHAYCTDIDRSGDVR
                     VLCNVKPTYGWMNTLLHENGHAVYSKYNDPAKPYFLRDAAHSFTTEAIAEMMGRLASN
                     PQWMLDMGIITPEQKEQIASECLKSLRLEQLVFSRWAQVMYRFEKEMYANPDQDLNSL
                     WWSMVEKYQLLKKPEGRNEPDWASKIHVALYPCYYHNYLMGEMLASQLNAYIAANVLK
                     ISDFNAASFVGHPEVGAYLKEKVFVPGCLYPWNEMIEKATGEKLTAKYYALQFVK"
     gene            complement(42001..42882)
                     /gene="dapA"
                     /locus_tag="PK839_03860"
     CDS             complement(42001..42882)
                     /gene="dapA"
                     /locus_tag="PK839_03860"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009160945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HOG71905.1"
                     /translation="MQNDRFKGLGVAMVTPFSSSKAIDYSATDRLLEYLVDGGVDYLV
                     VMGTTGEASTLTDDEKHELIAHVVEKNNGRLPIVLGVGGNCTSSVISSIKHFNLKGVD
                     AILSVTPYYNKPTQKGLEMHFRAVAESSPLPLILYNVPGRTGVNMSAQTTLTLANEVP
                     NIIGIKEASGNLNQMAYILRDRPDNFLVISGDDGLALSQMAMGADGIISVVGNAVPGR
                     FAEMVHFAQKGKFAEAAVIHLKLVELIDALFVEGNPGGVKAALSILGIIDNNLRLPLV
                     PVGEATLQKLNMLLKSL"
     gene            complement(42903..43466)
                     /locus_tag="PK839_03865"
     CDS             complement(42903..43466)
                     /locus_tag="PK839_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG71906.1"
                     /translation="MFDDYKYKKALNEINRQAQGFKRQRMVHNLTTARKILIVFTLNS
                     QEVLDSVLNLVKYFESLNITVHALGYYPQKEVPQVFTMHPGITIFSKAETNWYGKPLS
                     SDVEAFINKEYDILIDVDFDEVVPVRWISTFSKAKFKLGSQNYLNNPFDLILTVDKLK
                     GLPYLCEQVKQVLFKLNNRFADEPHQA"
     gene            complement(43713..45734)
                     /gene="ligA"
                     /locus_tag="PK839_03870"
     CDS             complement(43713..45734)
                     /gene="ligA"
                     /locus_tag="PK839_03870"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HOG71907.1"
                     /translation="MTAEEKDRVQQRIMFLRDELNRHNYLYYVKTAPEISDFDFDMML
                     NELVELEKQYPEFDDVNSPSRRVGSDSSAEFKQVQHRYPMLSLANTYSEGELRDFDNR
                     VRNALSEPPTYVCELKFDGVAIGLTYLDGRLVQAVTRGDGSAGDDVTANARTIRTIPL
                     KLHGSGYPREFEIRGEIIMPRAIFDELNREREDEGEQPFANPRNAAAGTLKLLNPSAV
                     AKRRLDSFFYYMLGEELPFDNHYQNLMAAKEWGFKISEHIQLCSNLECVFDFIKYWDL
                     ARKQLPFDTDGVVVKVNSYRHQAILGMTAKTPRWAIAYKYKPEQVSTVLLSVDFQVGR
                     TGAITPVANLKPVKLAGTVVKRASLHNADQVALLDVRIGDWVYVEKGGEIIPKIVGVD
                     LSKRSPLAKILEFPTHCPECGSKLVKIEDEARHFCPNQFACPPQIKGRIEHFISRKAM
                     NIDGLGEETVALLYDNGLVKDAADLYFLKKDQLLLLDRFAHKSAENAIRSIEKSKNVP
                     FYRVVYAIGIRYVGEATARSLAQHFGSMEAIAKATYNELVEVEDIGDRIAKSIIEFFS
                     NPANRQLVEKLKMAGLKLQVEQIENLQLSDALKGMSVVISGTFSKVSRDELKELIRQH
                     GGKNVSSVSASTSLLIAGNNIGPSKLEKATKLGVKIVGEDEFFKLIGQE"
     gene            45923..48076
                     /locus_tag="PK839_03875"
     CDS             45923..48076
                     /locus_tag="PK839_03875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HOG71908.1"
                     /translation="MKTNAIADKYRDLLRVLRGSCGKDELKKIRKALDLVVSLNSASS
                     TILHLLEVARICVGEIGLGAQAAIAAILHETLENKQVTRVEIEKHFDSKVAEIVEGYT
                     LISGLDPKDPKTQAELFRQMLITLSGNAVVILIKLADRLETMRSLSRAEPEVRQKRSW
                     ETFHLYAPLAHRLGLYPIKSEMEDLAMKHIYPKEYKFIVKKLRETTSSRNRFIRSFIE
                     PIEQELKNRHFDFEIKGRPKSVYSIFRKMQKQDVDFEDVYDVFAIRIILNTDPEHEKS
                     DCWQVYSIITDKYTPNPERLRDWISAPKSNGYESLHTTVLGPEGKWIEVQIRTSRMDE
                     IAEKGFAAHWKYKGIKQEQGVDEWVSMVRNILESPDGSFGDKTDLLQLNLNSKDIFVF
                     TPKGEIRKLPSKATVLDFAFDIHSEVGSKCTGAIVNGKNAPIKQVLQNGDIVEVLTSK
                     KQKPKKDWLNFVTTSKAKTRIKQQLREVENQEIARGREMLLRRLKNWKVDNPEEAIRN
                     INKQLKLKDPTEIFVLIAEGKVNLTSIKDILTSNIEPEAPKNVDATTAPARPTDTTGS
                     SSEFLIIDERLVNIDYKLAKCCNPIFGDEIFGFVTVKDGIKIHRQSCPNATQLNTRWG
                     YRIVKARWRGSSNDGAFQTSIMVTGMDELGMLNRISEIISKDLRVNMRNLNVNSKNGQ
                     FEATIQLYVQDKKHLEMLLYRLGAIPGVSKAERLR"
     gene            48183..48815
                     /locus_tag="PK839_03880"
     CDS             48183..48815
                     /locus_tag="PK839_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG71909.1"
                     /translation="MSRKSLILFTLLSLFGMAARGQLVQDLYKIYDPVFGNDDHDSTA
                     ILTPFGVIKPGVSYNLSVGTSYSSFGSGMGLSETYISPRVSYSPNQKLQVVAGISFSR
                     TGTHGLSTPSPTNISTGQALDNPYQAWAYTQYNFNNRFSVYAMGSVSQNQTYFSPYSR
                     TLGSYNSQMYGVGFNYRISSKASIGASFNFVNRNPFGYQLGGYSNPFIPY"
BASE COUNT        14318 a        10825 c        10528 g        13475 t
ORIGIN      
        1 aaaaaagaga gtcgcttatc agcgactctc ttttttagta ctatccatta gcaagttatc
       61 tgaagctaat tcctatgccg gtgttaagaa cagaataatc gccaaaggag taatcgcagt
      121 gaaacgtaat caacgcaagt tttatcttta taccaatatt ggcgttaaat ccattattag
      181 agaagtctaa cgcaagtgga tctttcatgt ttaatccaac attgggaata tcatagtatc
      241 ccttcaactt gacatctgta gttgacttta cgtatccgac gccagcataa acggtaagaa
      301 caggaattga ctttgaaacc agcagcttgc cggtaaaccc cgaagcgtta aagttaaggg
      361 tttgatcttt gcttttatca atatcaaaag tcgaggtaaa ctcggtgtag ccgaaaaaaa
      421 ccgaaacatc tataggtatc agcttaaatc ccggaataaa ttccttaaat tcatttttta
      481 caccaattcc ccaggagcta aagttaccta cctcaggaaa atcgaccttg gggaaaaagc
      541 gcccaaccac ctcggtgtga aatggcaaac ccagcccaac ctgaattacc ggggcaggaa
      601 gaaattgcat gtttgaaccc tttggcaggg tgagctcaga tagtcctgtg gccttatcgg
      661 taagtatagg gccgcttacc ttttcaccgg aaacagtagg tgaagtattc tgggatggat
      721 tcttcaactc ccagtattca aggttaagct tggccacatc aaaattttta gcggatgatg
      781 gcggaaatgt tactgccccg gtaaaggtga tatcgaaacc aaaaagtcca tgaggcttgg
      841 cagaattaaa ccacccgttg ttcaggcttg agcccagact cttaccaaaa ggctcgaggt
      901 aggctgctgc gagtttttca gcatcgttaa ggtttccgct taaaacctca actacatttt
      961 cctgagcatt aacagtgaat gtggcaacaa ttgtgctcag gaatataatc atcgaaattc
     1021 ttctatggct tttcatcttt tcacatttat ttaaaaggtt aatgggaggc atattttaca
     1081 gcaaagctct aatcaaatca gttttcaaaa ttaagttaga ttattttcct aaatcataag
     1141 caaaagtacg aatgtttcgt aaagtaagca tcattaactt aaaaaatatc gttggtaagc
     1201 tattagttgc tatttttgta gaaaagataa ggtagcatga gcagtatccc gctagccgaa
     1261 cgaatgcgcc ctcacacgtt agacgactat gttggtcaat cacatctggt tggggaaggt
     1321 agcgtgctaa gggatatggt aaaatcgggt aacctgaact cctttattct ttggggccct
     1381 ccgggggtgg gaaaaacaac cctggcaaaa attgttgcca acgagctaaa aaggcccttc
     1441 catattctta gtgcaataaa ctcaggggtt aaggaggtta gggaggtaat tgaaaaggcc
     1501 aagtctcaaa aattctttaa ttcaccctca ccaattctgt tcatagatga aattcaccga
     1561 tttagcaaat ctcagcagga ttcactttta ggcgctgttg agcagggtgt aatcacccta
     1621 attggggcca caaccgaaaa cccgtccttc gaggtaattt caccactact ttcacgctgc
     1681 catgtttaca tacttaaacc actcagcaac gaagagctat cccttttact caacagggcc
     1741 atcagcacgg atgttgagtt aaaaaaaatc aaatttaaaa ttgctgaaac cgaggcgtta
     1801 taccattttg ctggaggaga tgctcgaaaa ctttacaaca ttattgaact actagccagt
     1861 gttgctggtg gtaaaggtga agtggagata tccaatgaga aggtaaccaa agttctacag
     1921 gaaaatattg cgctctacga taagaatggc gagcagcatt acgatatcat atctgcgttt
     1981 ataaaatctg ttaggggtag cgatcccaac gcagctgttt actggctagc tcgtatgctt
     2041 gagggaggtg aggatccaaa gtttattgcc cgcagacttg ttatactggc atcagaggat
     2101 atcggaatcg ccaacccaaa tgcacttctg ctggcaacca gctgctttca ggcagtacat
     2161 cttattggta tgccagagtc tagaattatc ctatcagaag cctcgatata tcttgctacc
     2221 tctgcaaaga gtaattctgc ttatgaagca attgacaagg ctcaggcgct agttagaact
     2281 tccggcaacc tacccgttcc cctgcacatt cggaatgccc caacaaagct aatgaaggaa
     2341 ataggctatg gcgccgacta caagtacgcc cacagtttta aaggaaattt tgttgatcag
     2401 gagtttttgc cctcaggcat atcggggaca acattatatg agccacagga caacccaaag
     2461 gaaagggaaa ttgccatgag gttgaggaac ctctggaaag ataagtataa ttattgattt
     2521 gtaaaacacc tgtatatgaa tgttttaaac tcaatcacct caatcaagca tcttgattcg
     2581 gggaattttt ttctaatggc cggaccatgt gttgtagaaa gcgaggagct ggcaataacg
     2641 gttgcagaaa aagtaagcgc catagcggag aaatttaaga taccctacat ctttaaagcc
     2701 tcctatcgaa aagccaaccg ctctagaatt gattcatttt cgggtattgg cgacttgaaa
     2761 gcactgaaga tacttcaaaa gattagaact cagctacagc tacccgtagt taccgatatc
     2821 cacagcgccg aagaagctgg catcgctgcc gagtatgtgg atgttttgca gataccggct
     2881 ttcctctgcc gccaaaccga cctgctaata gccgcggcga aaacaggtag ggtggtaaat
     2941 atcaaaaaag ggcaattcgt ttcacctgaa tccatgaagt atgcagcgca aaaagttgtg
     3001 gattccggaa atagtaaggt aatccttact gaccggggca cccaatttgg ctaccaggat
     3061 ttgatagttg actttagggg gataccccaa atgagagcct ttggatttcc tgttgttgtg
     3121 gatgtaaccc attcgctaca gcagcctaac caaacaagtg gagttacagg gggaagaccc
     3181 gacctgatag agactattgc acgggcagca gtagctaccg gtgccgatgg tatttttatg
     3241 gagacccatc caaatcctgc tgttgcactt tcagatggtg ctaacatgtt ggatttaaaa
     3301 tatatggaag aattaatgaa ccatttggtt caaatcaggc aggtaatcag gaatttttaa
     3361 tgtaagcgtt tagtagctca ttaatctttt gcttgagaag caaggcatta ataggctttg
     3421 aaatgtagtc gttacatcca acctcaaagc agcggatgtt atcgtggggt aaggcgtagg
     3481 cagtttgggc gattatgggg acttcggcat tctgccttct gatggcggta accgcctcat
     3541 agccgtccat taccggcatc tggatatcca tcaggatgag gtcaaccgga actgctcccc
     3601 tcaaagcggc attaacagcc tcctcgccat tcctggccca aataacgttt gcctttgttt
     3661 tggcaagtat ggttgatatg tacaggtagt tggactccgt atcctcggcc acaaggatat
     3721 tcctaccctc ccagctgtac tccggcgagc tcgtcctgta gaacttggcc cccgaatggg
     3781 ctacatttac gtcctcgtag gggatggtga agtagaaggt agtccccacc ccgcaggttg
     3841 actcaaacca gatatcccca ccgagcaggt tcaccacgct tttgctgatg tatagcccaa
     3901 tgccggttcc cccatacttg cgggtcgatc cctcctgaat ttgccggaag cgctcgaaga
     3961 ttatatcctg cttgtcggta gggatgccaa tacccgtatc ccgaacaaaa aactctattt
     4021 gccgctcctc gggacggttc acgaagccaa actccacaaa acccttctcc gtgaacttga
     4081 gggcgttgct gatgaggttg accagcacct gtcggagccg gagcccatcg gtgtaaagaa
     4141 tgttggcctc accatcattc aggttcagca gccggatgtt gatgtgctcc ttttcctgct
     4201 taaactttac ctcgttaaag aatgtgtaca cctcaaacag gaggttatcc aaatcgcatt
     4261 ccgccttggt aatggccagc tggcccgcat caatctttga aatgtcaata atgtcatcaa
     4321 tcaggtttac cagcatcaac ccgttgtggt agatgatgtc aatatagcga tcacgtttct
     4381 cggccgattc ctcattcttc aacagctggg caaaccccag aatcccattc atgggtgttc
     4441 ggatttcgtg gctcatgttg gctaagaagt tagatttaag tatatcggac tcctcagcct
     4501 tgcgcttggt tctttcaagt gcctctctta acctggattg ttctaaggct tgtgtagaaa
     4561 gaaggctgtt tagctcatcg ttatctacct gcagctctct gcatctaatt ttcaactcgg
     4621 cattttctat ttttaatgct tcaatatcct ttagctggtc tgaactacct gttatgttcc
     4681 tggggtgaat tctacggtga attaaaacta catatcctac tagtagtatg caggtaattc
     4741 ctaatatcac gttcagtata gtcatgttat ttaataatct aaaatacaat ctgttgtaag
     4801 taaccagcca gtaatcaaaa cctagcagca caaatttagg gtaaataaaa atgttcacct
     4861 ttaaaaatac ccatttttac tttaacatgg ggatattgtt tattaacgta tcctaagagt
     4921 tgataaaaaa acctccatcg gaaaatggag gtttaaacgt tcccttttag aagtcttaaa
     4981 agaatattcc caggctaaaa ataaactggg ggttccgttg gtcgaattta aaatcactac
     5041 ctcccagggt aataccctcg cccagcttgc tcaggtttcc ttcgtattta aagtcaaagg
     5101 ttatcttctt aagaatatca atgccggcta ccagctggta tccaaacacg gcatttttaa
     5161 aattactttc ggctgactgg ctggttagcg aggcaagttc ctttttgagg ccgtcgtttt
     5221 cgctaagcat aatggatgca acaggtccta gtccgattct tgctgggcca aacttccaac
     5281 ctaccataac agggatatca atcttgttaa acttttgatc ttctacggtt tgaatagctt
     5341 cgtcggcact atttatgtat gacagcacaa cctccccttt tgactgggta aatagaattt
     5401 cgggttggat aaaaagtccg gcaagttgta ttcttccaaa aacgccaaaa tgaagaccta
     5461 gcttggagtt gccctgttca acagcaaatt gtttaagccc atcagtcgta gtgagcccct
     5521 gtaggctggt ttcgtcaatt tttacattgg tagaacttac gcctcctttc acaccaaatc
     5581 tcagaaactg agcatttgca attgtgaatg aaaaaagggc taggactaaa ataaatagct
     5641 ttctcatagg ataaaattta aggttagact ttaaagttgc ttattattaa gataaccact
     5701 catacaaata tcatgctaaa cattaaaaaa gggggctgat taaacgggcg aaagcctcct
     5761 taaagctgtt accagccgat ctcctgttcc attcttcaag ggtgatctcc tggctgctat
     5821 ttctgaactg aatgaatgaa ctttccagca agcgggtaat ttgatcgtca tacataattg
     5881 cagcaacttc aaagttatcc tcgaagcttc gaatatccat atttgctgaa ccggtaaaag
     5941 ccaacgagcc atcaatcatc attaactttg aatggttaaa gccatgccgg taaagataaa
     6001 ccttaattcc tgcctcaagg agttcagaaa cataggaaag ggaactccag taaacaatag
     6061 tagagtcgga aataccagga agcataattt gaacatcaat accgctcaat gcagccgttt
     6121 taagggctgt aagaatactt tcattgggaa taaaatatgg agtggcaata tatacatggt
     6181 gagacgcttt tgttatacca aggaaaaacg cttgcatgat gcttgcccag tcagaatcgg
     6241 gaccgctagt tgctacctgt atagggtggt acgtgctaac cgatacctca gggaagtaaa
     6301 gtggacgatc ggcaaccaaa tcgccagaaa caaaatacca atcaataaga aaaatcacct
     6361 ggagggaatg caccgcttca ccctcaattc gaagcatggt gtcaaaccac atccctattt
     6421 tgctaccctc aatgtagcgg tcggcaatgt tcatgccccc cagaaaagcc acacgaccat
     6481 caataagcac tattttacgg tgattacggt agtttgcctt actggtgagt agcggaaatc
     6541 ttaccttcat aaaagggaaa acctctacac ctgcctgttt tagctccctg atatactttt
     6601 ttggtaggct ccagctgccc acatcgtcaa aaataaccct taccttaacg cccagcctgg
     6661 ccttggctat caaaacctcc ttaatccggt taccaatttt atcatcgtta ataatgtaga
     6721 attccaggtg aatgctacta gttgcgttgt taagctcatc aataatggaa tcaaaagctg
     6781 gctttccttt gaagaatatg gaaacgctgt tgaaatcagt aagcaaggct ttactattat
     6841 tcaaaagtag cgttataatg cttatatgat ttgagatttt gctatttagc tggccttttt
     6901 tgctgaaatt ggtaacctgc tgtcggctca ggtagtcaat ttgttgtata tcctgaattt
     6961 cctttcggtt aaatattttt tcctttcggc gattttgccc gaaaattacg tagagaataa
     7021 gtcctagaac aggaagggag atgatcagaa gaacccatgt agaagtctta gaagggtcgc
     7081 gcttttcatg aataaccatt atgatggtga aaagaacggt aataaaataa ataatcagaa
     7141 taatccggtt aaggttggcc atagcgacat tcatgaaaaa gttaagcaaa atcattgcct
     7201 atgatttagc caaccaatgt ataaaaaaaa taggaatttg acagcttaat aaatagctgc
     7261 aggtaaaacg gctatttaaa cggagaattt ggttctcggg ccatgaacat gtatagtatt
     7321 cggtcaataa tcttagggaa gacccgttgc gccagcacag atagcttacc ttcggttgtc
     7381 atgataatat ttcgcctttt cctcagtatc ccatgagcaa ttgttctggc caccttctgg
     7441 ggagtcatca tgtgcttttc gtcgcgggga gacacgccct gcgggcttcc atctgccgtg
     7501 agagcggaaa ctctgacgtt ggttgcagta aatcctgggg ctgctaccat cacatggagg
     7561 ccatttttaa gattctcgat acggagtgtt tccagaaaac cctgaagagc aaatttagaa
     7621 gctgaatatc ctgagcgacc cggcagccca tgaaaaccag caattgagct aactccaata
     7681 acagagccgc gacttgccag aaggtggggt aaggcatact tggtgcagta aacagcgcca
     7741 aaaaagttaa cctccattag ctgtctaatt acctttaaat caacatcctt gaaaagtgca
     7801 cgcatcgaaa tgccggcatt gcaaagcagg taatcgattt ttccgtatcg tttcagagcc
     7861 tcatctacta aattcttaca atcatcctcg actgttacgt cggttctaac tgcaatggct
     7921 tcatgcccgt taagcaacaa atcctgaaca atgctattca gcacttcaag attacgggca
     7981 gctaaaacca gctttgcacc tcttgatgca aattcgtatg cgcaagcctt tccaatacct
     8041 gaagaagcgc ctgttataat ggcaatcttc cctttcatgc tagtttgagt tgacataaaa
     8101 attagctata atatactaaa gtttaaaaaa atattctccg cactaaaaat aagtaaaaca
     8161 tggagaatta gaaagtgttg cgaaactttt aaagatatct tgcacaagat tataatgcga
     8221 tgtgctcatt ttcaatgttg tataaatttt ttatttggtt tgtaaataat tcaacgtaag
     8281 aaattggatg tttaggtaaa aaattaatta aatttgcagg cctttaggca aacttatgta
     8341 acataagtta taataagatt tgatagaagg attcagtagc tcagctggta gagcacatcc
     8401 cttttaagga tggggtcctg ggttcgaatc ccagctggat cacaagttat aggctattca
     8461 acattaccat ccataatcat agtggatggt ttttttatgt acagattcca aaattcacag
     8521 cctgcaaatt aaatagagcg tagatttcaa atttttactt cctacagccg gattcctgtt
     8581 tccattgcct taataagcta atgctcaaca aaaaagccct acgatgtagg gctcttatta
     8641 ggagtaataa aaaacgtatc tacttggaaa tgagtatctc aaatgagttt ggctttaaat
     8701 ctactttaac aatttggttt tgaatggaag aatttgagcc aaactttgag gtaaagtttc
     8761 tacccaccat ataatcaggc aatttaaaaa ttatagattt atcctcatca ctcttattaa
     8821 agacaactat tgcagaactt tgcaggtatg ttctggcata agcatactgg gttgagtcgg
     8881 ctaaaagggt ttcaaagtta ccatagttaa gggccatggt gtttcttcta aggttcacca
     8941 gtttttttac aacatcaagt gtcctgttct ctattggtga aaggttatca aatttcatca
     9001 tcctgcgatt atcggggtcg tttccaccag gcatgccaaa ttcatcaccg tagtatatag
     9061 tagggacacc gggaatggta aggttaaatg cgtttagcat gcatagtttg ctatatgctg
     9121 atgtgtcgcc aacgccaata tctcgtgtcc atccggcctt tttagcatcc tcgtcgaaac
     9181 gtaaagcacc ccctgcatag gagataaagc gccccctatc ctggttaccc gaaatgtacc
     9241 ccattaggtt gttccagcca aaatagctaa aggactctag caaagagctg tttagtgatg
     9301 aaaatgggta gttgcttcga gcaaacgcgg caaccgaggc atcgtaaatg ttaaaatcga
     9361 actgcgaatc aagcattccg gaacctatat agcttgcaac cagctccctg ctaccatagg
     9421 tttcaccaat ttgatagatg cttctatcgg taaccttgag cttaatcttt tgggtaagcc
     9481 tacgccagaa attctcatgg atgtgcttgg tagcgtcgtg gcggaaacca tcaagttgaa
     9541 aatttgtaag ccagtataga gcagtatccg tcattggctc gtaaacttcg ggacgctgta
     9601 ggtcgagagt tggtagaaat acatcaaacc atgtagttag gcggtattcg tcccatcgtt
     9661 cggtgtttag cgaaccatct ggaaggtaga gagatgttgt ccaatcagga tgctgcttat
     9721 aaagggggtg ctctacatga atatggttgg caacgtagtc gagaatcaca ttcatgtttc
     9781 ggctatgcgc ctcatcaatc aactcgcgca agtcgccgtc tgttccatag cgaaagtcaa
     9841 tttttgtaag cgaaaccggc cagtatccat gataaccgga gaattttgtt cgtggattcg
     9901 ggtacaaacc ccatgctcca agaggatttt gggttatagg agatagccat attgtattga
     9961 ttcccaaaga agtaaaatat ccatctttaa tcttctgtgt aactcctacc aagtctcccc
    10021 caaagtagtt tgccttggga agaatttccg gatcatcaac cttcctgtcg ttagccgtgg
    10081 tgccgttgtt gaagcggtca accataagaa agtacataac ctgagtatgc ttatcgctac
    10141 gggttagctt cccagggtct tcaacaactc tacaaccctc cagcgggata agcaggtcgt
    10201 tggattcgcc aaactcgtta taagcccata cacggatgta ggaacggcta tactcctcgg
    10261 catttcgggg taaggtaaca tgaaacacat cgccgtccct tttaataaag ttaccggtca
    10321 ataaataatt ctgccacagc actataatag aatcaaccgt tccggttgct ttaatgttaa
    10381 ggatgttctt gtgcgctgtt aatgcgacaa gtttgggtag catgttaggc tttaagttgc
    10441 caacttcaac aaccgagttc tgcccaccca tcccgttatc ctccttaagc gggttggcgg
    10501 gatcgagcat ccactttcca ttaacaacaa cttggtagtg atatttccca gggttaagca
    10561 gcagggttgt tctaaaaacc ttaccatcaa atgttaagct ggttgcatta gggttccaat
    10621 cgtttatttg accggcaagt tgaacggatt ttaccttttt accttccggg tcaaaggtaa
    10681 aggcaacagg aagttttttg ctcttgcgga caagtaaatc gtagcgttca ttccccttaa
    10741 cccacacctg tataatgctt aatggaggca gatttaggtt gtttgctacc agagtgatgg
    10801 aagcgctatc aggtgagtag ctataccgaa agaaatcggg aatagttata gagtcaacaa
    10861 ctttattatc agggaagtaa tcggtaagaa caactttagt actatcgccc ttcagctgaa
    10921 tggctgaaag caaatccgca acagccggtt tttccgcaac accatcaaga tttggcgacg
    10981 gttgacaagc tgctagggtt gcgattgaaa taataaataa taacttcttc ataaactgac
    11041 atttatgaaa tgataacttt agtataatca acgaagctcc aggatcaaag cggagcgtgg
    11101 cgagagctga attttctcaa gattgtcgaa taccttaccg gaaataatat caaccgctga
    11161 attctttccc ttcatcattt cgctgaagcg ggcagtttca agcattttag gttggtagcc
    11221 gttattgaga attaccatta cagtttgctt atcgtcaaac cggaagtaaa cgtacacttc
    11281 attttcgggt ataaaatggg ttaactttcc ggaatgaaca gcagaagccg ttttgcgcca
    11341 gttaagtata gtagaaatat agttaaaaat ttcattttgg cggtttgtcc tgccatcggt
    11401 tgtaaatgct gactttaaat caccctccca accgcccgga aaatcgcggc gaatatcgcc
    11461 atgtcccttg gcatcatcgc caggcaggat tatttcggtg ccatagtaaa tctcaggtat
    11521 tccacgggta gtaagtagaa atgccattgc caattttagc cggccaagag ctagggatga
    11581 gtcccgatga aaacgtccaa tgtcatgatt atcagcaaag atgagaaggt tgttagggct
    11641 tgggtacaca aaatcatgag ctaaaatttc atataggcgg gcaagtccgg tatcccaacc
    11701 atccttctcg tcaaaagcct tgtgcatggc aaacataagc gggaagtcca tagccacttt
    11761 aaggttcgag ttgtaaccat cctgattatg ggcatccttt tgccagtagg caacccaatc
    11821 ggggtagttt acccatgttt cgccaacaat actgaaattt gggtattcag acataacagc
    11881 agcacaccaa cgcgacatga aatgcttgtc gcagtatggg tgggtatcca tccgaatacc
    11941 gttgagatcg gcatactcaa tccaccatat gctattctgg ataaggtagg tagccatata
    12001 gggattgcgc tggtttaagt cgggcatggt aagatcaaac cagccatcgg ccataaggct
    12061 actatcaaca gttgaagcat gagggtcaaa tgttgtgggg atacggtaat ttgtccgcgt
    12121 aaactgagga aactggttta tccaatcgtg catgggcagg tcattcatcc accaatgaga
    12181 acttccacaa tggttaaaaa ccatatcctt caccaccttt aatcctcttt cgtgagccat
    12241 ttgggagaat ttacgataaa gctcgttatc gcccaagcgg ggatccacct tgtagaagtc
    12301 ggtaatggca tatccatggt aggaagttga aggctggtta ttctccaaga tcggatttag
    12361 ccaaagggca gtaacgccta gctgattaaa atagtctaag tggttaataa ttccctgtat
    12421 gtctccacca tgcctgccat aaggatcgtt ccggttgtac ttatcggaga aacccttaat
    12481 tgagtcgtta gcgggattgc cgtttgaaaa gcggtcagga aaaacgaggt agattacatc
    12541 ggaattgtta aagcctatcc tctccgacga accctgctta cgttctttca actcgtaggc
    12601 aaccaccctt ggttttccct ttggaaattt aaaggtaata ttgaaagccc caggttcggc
    12661 attctcatta atagcaagat tgagaaacag gtagttgggg ttttcagtct tgtcaacgct
    12721 ttcgagtgta acccggctgt ccgagattcc tacctgggct tggttgatgt tactaccata
    12781 aatcatcagc tggagcctgg ggttcttcat gcccacccac cagcatggag gttcaaccct
    12841 tttgatattc tgcgagttga cattcattga acatgctgca attagcatca acatcaaaat
    12901 taatggtttc ttcatagtaa taatgctttt aatgttaaac aaaaagggtt aaccccagag
    12961 gattaacccc aataaattac ttatcaagtt aaaaagttga ctctcaagtt acagaatgaa
    13021 tactggtatc ctgtgaagca tccgttcatg gctaagcacg attaagcgca ttcaaatcgg
    13081 caaaagcctc ctttaggcga ttaatcaccg actcctcccc ttttcggagc catacacgtg
    13141 ggtcgtagta ctttttgtta ggcgaatcct ccccttcggg atttccaatt tgcccctgga
    13201 ggtactcctt tttagactgg tagtaatttt taataccatc ccagaaagcc cattgaaggt
    13261 cggtatcgat attcattttt accacaccgt agctaatggc ttcagcaatt ttttcctttt
    13321 cggaaccgct tccaccgtgg aatacaaaat ttaccggttt gctaccctta ccaaatttcc
    13381 tgctgattag ctcctgagaa ttctttagta ttataggatt tagcactacg tttccgggtt
    13441 tataaacgcc atgtacgttg ccaaacgaag cagcaatagt aaagttggga cttactttca
    13501 tcaactcttc gtaggcgtag gcaacatcat caggttgggt gtagagccgt gagttgtcca
    13561 ccccggtatt gtcaacacca tcctcctcgc cgccagttac tcccagctct aattcaaggg
    13621 tcatccccat tctactcatt cgttcgagat agcgcttaca aatctctata ttttcatgaa
    13681 ggggttcttc ggataggtcg agcatgtggg agctaaatag gggagcacca gtttccttat
    13741 agtacttttc gcccatatca agaagtccat caatccagag cagtaacttt ttagctgcat
    13801 gatcggtgtg taaaactact ggtaccccgt aggcttctgc aagcaagtgt acatgctttg
    13861 cgccgctaac agcgccaagt atgctggcgc gttgtccgtc gttggaaatt gattttcctg
    13921 agatgaacga tgcaccgctg tttgaaaact gaatgattac aggtgatcca acttcatgag
    13981 ctgcttccat tacagcattc atagtgtttg tgccggtaac attaacagcc ggcagagcaa
    14041 acccttcctg cttggcgtat tcgaatagtt tattcaactc gtttccggtt ataatacccg
    14101 gtttaaaatg catattcccc attatttagc tttattagat gttttagtaa acttgttttt
    14161 gaacggcaat ggtaatagcg cccccttcag gaattagctg gtccttccca taaattttaa
    14221 cagtgatgtt gctacccact tcgttgaaga ggcttaactc cgagtttgta acccttattt
    14281 tgaggacgtt accacgaaac caaactttaa agctgaatga cttccagttt tgtggaatta
    14341 aaggattaaa agtgagcata ttactgcgaa cccgcatgcc accaaatcct tccacaattg
    14401 aaatccacgt accggccata ctggtaatat gcaggccttc ctttacctcg ttgttgtaat
    14461 catccaaatc gaggcgggct gttcgcaggt agagctcata ggctttttca acgttaccaa
    14521 ttctggaagc cagcacacta tggacgcagg gtgaaaggga agactcgtga actgttctat
    14581 tttcgtaaaa ttcgtagttt cgctttatgg tttcggtgtc aaatctatcc tcaagaaaat
    14641 ataggccttg cagaacgtcg gcctgcttaa taaagcagga acgtaaaata cggtcccagc
    14701 tccatttctg gtttattggc cgttccttgg gatctaaatt cttaaccagt atctgctcct
    14761 tatccaggta accttcctgc tggagaaaga ttcccaattt tttatcttca ggaaggaaga
    14821 tgttagagcg aatttccttc cagcgggcaa tctcattcac ctcatcaaaa ccaatcctgc
    14881 tgcaaagctc ttggtacttg gcattgttgg ttgactttac ataacttgat acttcttcag
    14941 cgtactccat acaccagcag gcaattgtag aggtgtacca gttattgtta acgttatttt
    15001 cgtactcgtt tggtccggta acacccagca tcacgtattt ccccttttct gtagaaaagt
    15061 tgactctttg cgaccaaaaa cgggcaattg caatgagcac ctccagacca aaatcaacca
    15121 ggtaatcctt atcgccagtg tagcgaacgt agttgtatat cgcaaatgct attgcaccat
    15181 ttctgtgaat ttcctcaaag gtaatttccc actcgttgtg gcactcctcg ccgttcatgg
    15241 ttaccattgg gtaaagagca gctccgtcgg tgtatcccag cttatgggca ttctcaatag
    15301 cctttcccag ctgcttgtag cggtaaagaa gaagctgacg ggcaaccttc tgtccgtggg
    15361 taaccatgta aaatggcagg cagtatgcct ctgtatccca atatgtagag ccaccatact
    15421 tttcgccggt gaaaccttta gggccaatat tcagacgttc gtccctacca gtgtaggtct
    15481 gacttagctg aaaaatattg aagcgaatac cctgttgggc ggccacatca ccttcaatgg
    15541 tgatatcact atggttccaa atatccgccc aagcttttcg gtgctcatcg aacagcttgt
    15601 cgaaaccctt ttcgcttgcc ccctcaataa gtgtttccag aactttggta aattttccct
    15661 tttcatggtt caggttggat ataacagccg catacttgta gagtatcacc tcatcgccgg
    15721 agctagcatt aaccgtaaac ctgttgtcaa catacatctg cccggagttg acctgtggtt
    15781 ttaggttttg aggttgccca ttaataaaaa gcaagtttcg catggcatgt cctacctgaa
    15841 aatcgagttt tttagtttgg ctgattatgt agctaaatat ttcaccgccc cttacatcaa
    15901 taacatccca gaatttctcg ccgtagtttg agtcttcgtt atgcacatct gcatcgaggt
    15961 aggagctaat ttgaatggaa gcattggagt tgacgggttt aatgctgtaa cgaatagcac
    16021 ctatttcggt atcgaccatg ctcaaaaaac gggttgactt cacctccagc ttgatgccgt
    16081 tgggtagctc acaagtgaat gagcgttcga ggtagccttc cttcatgtta agaatcctgc
    16141 aaaaattaag cactttagca gtagccaaat ccagctcctc accatttacc tttaccgaaa
    16201 tgccaatcca attagtggag ttgattacct tggcaaagta ttcagggtag ccatttttcc
    16261 accagcccac tcgggttttg tcggggtagt aaactcctcc aatatagctt ccctgaaggg
    16321 agtttccgct gtacttttcc tcgaagttag ccctttggcc cattgcacca ttccctatgc
    16381 taaaaatact ttctgaagca cgggtatttt caggaataaa attatcctcg atgatgcgcc
    16441 attcatcatg tttaaggtat gtattcataa gagtatgtat gttactattt ttctgataat
    16501 gaattgacta aatattgaaa tgtaaaattt ttcaggttgg gtacaaccaa atttgccctt
    16561 tttagtacct gagggtttcc gatacccacc acgcgcatgc ctgcatttat tgctgcttca
    16621 actccggctt cggcatcttc aaatacaata caacatggag gctctacacc aattgctttt
    16681 gacgctgcca aaaaaatatc aggagcaggt ttagcaatcg aaatgatatt gccgtctata
    16741 atagcatcaa agtgatcttt tagtccaatt cggtccatga ttaggggtgt atttttactg
    16801 gcagacccaa gcgccagctt aattccttta gctctaagtt cttccagaag ttctttggaa
    16861 ccgggaagaa tttcattcgg ggtcatccga ctaatgtact ccacatacca ctcattcttt
    16921 ctggcggcaa gttgttccat cttttcctgg ggaaaggaaa ggccgccaac ctcaagaagg
    16981 atttcgagtg atcgcatcct gctgaccccc tttaaccttt cgttgtggct ttcatcaaat
    17041 tcaaacccta gctcattggc caacctcttc catgcctggt aatggtactt agctgtgtca
    17101 accacaacgc catccaaatc aaagatgcat cccttaatgc tactcatttg gtaaaacttt
    17161 actattatcc ttaacaaaga gcattgctga agcagaagcg agtatcatgg aaaatccggc
    17221 aagtagaatc atgtagatgg cttcatcgtt gaaaaaactc ttcaacatag ttcccagcac
    17281 gataccacta acaatttgcg gtacaacaac agtaatgttg aaaattccca tgtaaacgcc
    17341 catcctttca gcgggcagag agttagccag aattgcatag ggcattgcaa gaattgctgc
    17401 ccatgcaata cctattccca acatggaaat aataagagca tactgattat tgaaaaggta
    17461 gatggaaaga aaacccaagc cgccaagcag cagagaaata gaataagttt taattcttcc
    17521 aagcttgtcg gcaatcttat taagaaaaat ggcaaatagt gcggcaaata cgctgtacag
    17581 tccaaaaaga accccaaccc agttggctgc ttcgttgtag gcaaaggaag tggaatcgcc
    17641 tatttcagtg ccccagatat gctgggcgat ggctggagtt gtataaaccc acatggtgaa
    17701 aagggcaaac cacgaaaaga actgcaccac tgctaacctg tgcatgatgt ttggcatgga
    17761 gagcaaggtt ttgaaaaagt ttgattttcc cttttttgta ggagtttcct cgagggtttg
    17821 accgctatac tcggcaaatt ctttaggagg atactctttg gttgtgaata ctgtccacaa
    17881 caccgaaacg agcaggagcg caccgccaat atagaacgac catattactg aaggaggaac
    17941 atgcccttcg ggggctgtgt tgccaatacc caaaacctca gtaaggaaat agggcaaaaa
    18001 cgaaccgata acagctccta aattgataag cagggtttgc acggagtatc caaggttggt
    18061 ttgctcagcc ggcgtcatat cggctaccag cgaccggaaa ggctgaaagg taacattgaa
    18121 cgaggcatcc attagggcaa gcatgatagc tccaaaggct accggaggaa taatgagaac
    18181 tgcccattgg gagttgggca tgaataacat ggctagagtg gcaacaatcg cacctccaaa
    18241 gataaatggt ttacgacgcc ccattcgatt ccatgtttta tcgctagctg ccccaaccca
    18301 aggttgaatt atcaacccca ttataggagc taaaagccag aagaacgaaa gatgatgcag
    18361 atcggcgcca tagtttgaaa gaatgcgcga tacattggcg ttttgaagag caaatccagc
    18421 ttgaactcca agaaatccaa agcttagatt ccaaatttgc caaaaacgga gtttaggttt
    18481 ttttgccata tcaaactaaa ttttttcata atcaccaaaa gtaatttaaa aaaggttaac
    18541 taaaccttaa tgaaaactta aaacctttat aggcaaaggg attcagaaca cgcaaacagc
    18601 atttcaatcg tttacggtgg aataattttt ttttaaaaat tttcaatttt tcttcgaaca
    18661 gctttccatg ctgaaaataa agctaaagtt catctaaaaa agtagcatca ttaggtaaaa
    18721 gaaaaatcat agctacctat cagatattca ggcaaataga tagtgccaaa ccagtattgt
    18781 gaattgcctg ggtggttatt cctgcatttt gggctgtttg cactagtaaa ttttactgtt
    18841 ttctactgaa aataggtagg atcataattt cagtagcaaa acaacacaaa atcacaccct
    18901 atttatatgg ggcatttgga gttaaagggc agttctaaaa ttcagaagtg aagtagaatt
    18961 tgatatcggt gaacttatcc tgagccattt gcagggcgta gggagattcg gcaaggtaaa
    19021 cttccctacc ctgcttatcg gtggcaagat tactatactt acgaatacga aaatcatcaa
    19081 gttgagcttt attgctactg acaatccagc atgctttgaa aatattgagc ggttcatact
    19141 tgcactttgc gccgtactca tgctccaggc ggtactggat aacgtcaaac tggagggctc
    19201 caactgtacc tataatttta cgaccattag aattttgggt aaacagctgg gccacccctt
    19261 cgtcagtaag ctggtcgata cccttagcca gctgcttata cttcaatggt tcagcattct
    19321 caatatacct gaatagctcg ggagagaagc tggggatacc tttgaaatta ataatttcac
    19381 cctcagtaag cgtatcgccg atcttgaaat ttcctgaatc gggcaagcct acaatatcac
    19441 cggggtaagc cacatcaact attgattttt tatcagccaa aaaagcgttg gggctagaaa
    19501 accggaatgt tttcgccatg cgaacatggt agtacggaac attacgttca aatttacccg
    19561 agcatacttt tagaaaggca atcctattgc ggtggttggg atccatgttg gcaaaaattt
    19621 taaaaacgaa gccgctgaat ttttgctcaa agggtgaaat tgttcggatg tcggcctcgg
    19681 caggtctagg atagggtgcc agctgcaaaa aacaatcgag aagttcttta acgccaaagt
    19741 tattgagggc gcttccgaaa aaaaccggag caaccctgcc cgaaaggtag gtattgatat
    19801 ctaacggagg ataaacttcg ttaataattt cgagttcctc cctgagcttt gctgtatatg
    19861 gcttaatata tttatccagc tcgggagaat tgatatcagt aatttcaacc acctcgtcag
    19921 ctaccttttg tttgaagtcg ggtgaaaaca ggaaaagttt ctgctcaaaa aggttataaa
    19981 cacccttaaa agttgggccc tgtccaatgg gccaactaag gggcctaact ccaataccta
    20041 gctctttttc cacctcatca agtaaatcga aaggatcgtt tcccggccga tcgagtttat
    20101 taataaaaac gatgacgggg gtttttcgca tacggcaaac ctccatcagc ttacgggttt
    20161 gggtttcaac tcctttggcg tgatctatca caatgataac gctgtcaaca gcagtaagag
    20221 tgcgataggt atcctcggca aaatcctggt ggccgggagt atcaaggatg ttaattttat
    20281 acccctggta ctcgaactcc attaccgagg ttgacacgga tattcctcgt tgtctttcaa
    20341 tctccatgaa atcggaagtg gcagttttct taatcttgtt gctttttaca gcgccagcag
    20401 tatgaatggc tcctccaaat aggagaagct tttctgttag tgtagttttt ccggcatctg
    20461 gatggctaat aatggcaaat gtcctacggc gcttaacctc ttcctcaaag ttcatacctt
    20521 acttttttgc agccgcaaat gtacaaaatt tagcattggg tgttgaagtg aatgtataag
    20581 cactagggtg atattctcaa gcctccagct taccttcact tttagcaatg caaacggcaa
    20641 tcgtggcgtc tccggtaacg ttaacaacag tgcgcagcat atccaggggg cggtcaatgg
    20701 cgaggattag tgctagccct tcaacaggga ggccaacaga attgagcacg ataatgagca
    20761 ttacaacccc tgcacctggg actccggctg aaccaattga agcaagggtt gcagttagca
    20821 aaatggttag cagctgcgaa aatgttaaat ctataccaaa tacctgggca ataaaaactg
    20881 ttgctacagc ttggtacaag cttgttccat ccatgttgat ggtaacgccc agtggaagca
    20941 caaagccaga gatatcctcg tgaacgccca gctcgctctc aacctgcttc atggtaaccg
    21001 gaagggttgc cgcgctcgaa ctggttgaga aagccaccag ctgagcagga aacattgatt
    21061 tgtagaattt tccaaaaccc gtaccggaaa tgatttttaa aaataatggg taaactataa
    21121 caataatgaa tatgaggcca ataataatag tgagggcata cattcccagc gaggcaaaaa
    21181 gctgaaggga ggaagagagg ctatccccag caaagtcaac aataagggaa gccataagag
    21241 caaaaacgcc aaaaggagca aaaaacatga tgatttctac aatcttcagg ataacctcgt
    21301 taagcccctg gataaaattg ttaacaactt caaccttcgg gtttctaacc agcactaagg
    21361 ctatgccgaa aagcagggcg aaaaaaataa cctgaagcat ggccgtattg ctcgatgcag
    21421 cctgaacaat attctcagga acaatatcaa ccagaaactg tagtggagaa tcctgagaga
    21481 tgctctcggc agcttgttgc cgagagctaa tgtcaaggga atacttagat acaaactcct
    21541 gcctgcggtc atcgctgaag tagctaccgg gttttatcag gctaaccaag cttaggccaa
    21601 tggtaaccgc cagcaaggta gtgattaagt acattgcaat ggttttaatc cctattctgg
    21661 agagtttgcc tacatcagtc atcccggcaa ttcctgtaac tagcgaagca aaaattaacg
    21721 gcatggcgac tagcttcaga agattaagga aaatggtacc aaagggctta atgtagtcgg
    21781 aaaccagctg gttagcgcca aggggaattg caataatgcc cagtataagg cctaataaca
    21841 tacctatcag gattttggca tatagtggta atcgtcgggc tcctctcata aatatgaatg
    21901 ttgaaaattt ctactttaaa atcattgact tgccctaata aaggctactg ttttgttaaa
    21961 catatattaa aaagaccgga caaaccggtc tatagcacaa gttgagtata ggttaacgct
    22021 aaagagtatt gaacacacct ttatactcgc cgacaaaatg aagggaatcc gcatttacca
    22081 tgttaagctt gtagctcata agccgtccaa ggttctcaac tttaaaaacc cctgctttta
    22141 caggataaac cttaccggtg tcaacatcga agttgtagtt gaaatagaca ctgcacgagt
    22201 cggccgaata aacctgtttg gtagaaaaaa tatcaatggt gtagtctcca tttttgacct
    22261 caacattgct ctcggagtac atatatagcc taattaggga tcctgttccg gtgaaagcgg
    22321 cgttatcagt gctgtatttt acgccctcac caaggagaga taccacgaaa ggggtgcttc
    22381 cggtttcctg caaggtttcg gaatacttgt actggtatcc taagtaaaca cgaaactttt
    22441 cgtttccaaa tcggatgaaa ttatcggact catcatcgtc gctacctccg catgaagtga
    22501 aagcaacaag aactattccg ataagaagtt ttactaaccg gtttctatgc atacgagtag
    22561 gtatgtggtt gtaattaacc ctgctaaaat aaaaaaaaat tattacaaaa aaggtaaata
    22621 aaaaacggga aattaaaaat ttcccgtcag tgtttacgaa ttgaagagtt taatggtgcc
    22681 catgagcgtg aacatggcca tggctgagct cctccgaagt ggcttccctg acatcaataa
    22741 tttttccggt aaagtaaagg tcttctccag ccaaggggtg gttgaaatcc attctaacat
    22801 gggtatcgcc cacctccaga attgtgccat ttagctcgtt accctcggaa tctctcaacg
    22861 gcaatgtgtt tccaactacc aacatgtctt cctggagcac tccatcaata acgaatatat
    22921 ccttaggaag atcaatgatt gcctcctcgt caacttctcc ataaccatca gcatagggaa
    22981 tcgaaaattc gaatgaatca cctgttttca ggttgtgaat gttggtctcg aagagaggca
    23041 gcagctggcc atggccgtaa aggaaaacta acgggttgtc agcaaccgcc gaatcaacta
    23101 aatcgccatt gacttttaac tcatagctaa tggatacaac tttgtcatta gaaatgttca
    23161 tgtaaattga tttgatttaa ttaataattg ccaaaggaat atctaatttc ggtaaaaaac
    23221 aatagaaatc cccaaatgtt cgaatgaaaa agcgacctta cttaaaagca tgtatatccc
    23281 ttgaggcaaa gattttaaca ataaataatt ttttttttta gaataatgtt gaatattcaa
    23341 ttttatatgc catatttgca acgctattaa ttaatttaat tcatcattta tatcaatttt
    23401 aacatgaaag gtaaagtaaa atggtttgat gcagcaaaag gctttggctt cattcaaacc
    23461 gaggaaggta gtgatgtatt tgttcactac acaggaattg ctaaggatgg tttcaagcgt
    23521 cttgacgaag gtcaggaggt aagttttgaa attaccgaag gaaaacgtgg cccacaggct
    23581 accaatgtgg tagtgttaga ataagtttat tcctaacata ctagccagta aggcctccaa
    23641 tggaggcctt tttttattag aaggtaatgg taaatcgcaa aatgggattg ctgttgtagg
    23701 gtgaataccg gttgtcggtc aaatcccaca gcactaaaaa gttaatggca gccctatcgc
    23761 caatgggttg gtgccaacct cctccaacca ggtacatatt cacccagcct ttcccatagc
    23821 tgctactctg gtcaaaaaag tttcggtcga ggtaaagact ttcaaattca ccatgcaaaa
    23881 taattccccc atgcagatca atgccaaaag tcctatggaa atcggtaata ggaataacct
    23941 gggtaaatcc cgacactccc cagacgtgtg tctcaatagg ttcggtggtg aacccattaa
    24001 tgctaaagcg ttcacgacgg tagctgtagc gcccgcctaa ccctaaagac cactccggga
    24061 aaatccatat acccatcatg ggggcaacat caacctgcgt aaccctgcta ccaacagcca
    24121 atccaaaact tcccccaaaa aatagcctat cggaaaatcg gtcaaaattt ctaccctgtt
    24181 cctggtcctg cgctcttgct gaacctatca acataactcc tgtggccacg aggagatagg
    24241 aaagaatttt aattgaatgt ttggttaagt tggcaattat ttttctcata cggacaacac
    24301 taatcaggtt tatgaaataa agtattacct tccctttttt aattagccac ccaggatagg
    24361 gtcaaggtaa tgccgtatat acggaacgga aatctgcctg ctcgtgcaat tttatcaaaa
    24421 caaaatgaaa gaaaaaaggt tagtaaacaa aaaattatga atattattgt aaccggagcc
    24481 tccagaggta ttggatacca aacagtactg cagcttgcga aatcggcagg aaacagaatc
    24541 atagctctag ccagggacca tgaacagcta acaaggctgc agctagagag cagaaaggtg
    24601 cagcctgaat cggaggtaat acccttagtc tttgatttac agcagactag cagttacgat
    24661 gacgcattga ttcctcaaat caaaggacat tttacttctg ccgatattct aataaacaat
    24721 gctgggttgc ttgtaaacag gacatttgag cagcttactc ttttggaaat ggtgcaaatg
    24781 ctgaccgtaa actttatagc gccagcctac ttgacaaaat tgattattcc attaatgaca
    24841 aaaggcggac atgtagtgaa cattagcagt atgggaggtt ttcagggaag ccagaagttt
    24901 aaaggtcttt ccatatactc cgcttctaaa gctgcgctgg cgtcgctatc ggaatgcctg
    24961 gcggaggagt atataaataa aggtatttcg tttaattgct tggcgctagg agcaaccgac
    25021 acagaaatga tgcgcgctgc atttcctggc tataatgctc ctattaagcc gaatgaaatg
    25081 gccaaattta ttgccgaatt tgcaattaaa ggtaaccagt actttaacgg gaaaattatt
    25141 ccagtctcga cttcaacccc atagtcatta cattattttc actgcatatt aataatcaac
    25201 ttactacatt tacttccaat gccgatatag cttacgaata atacaggtaa aacaatacat
    25261 tcaattattc gcaagtcaac tacgtgtatg gtgcaaagta caacgataaa aaaagagaga
    25321 ccatcacaag tctcccttaa ctttaagcga atagtagtag attctgctgt tacctacctg
    25381 taaattttgg gaattacctc cccttttagc accattaggg cattcatggc aagggcttgc
    25441 atttcatcct ctcccgggta aacggcaata ggtgcaataa aaccaaccat ttcctttatg
    25501 tattcaacca agtcagggtt atgcgctatg ccaccggtca gaacaatgga gtcaactttc
    25561 cctttcaaca cggtagccat ttcgccaatt gccttaccaa cattatatgc catggcatcc
    25621 tgtagcaact tagccatagc atcgccacct cgagctttaa cctcaatttc ataggcatta
    25681 ttagtacccg tgtgagcgac tagtccacct tttccggtga tcattttttt aacttcgtcg
    25741 taggagtatt ggccgctata gcatagtttt gccagctggc cagcaggaag tgttcctgcg
    25801 cgctcgggcg agaaaggccc gtcgccgtcc agtgcgttat tcacatcaac aacccttccg
    25861 ttatgatggg cacctacaga gataccgcct cccaggtgag caacaatcaa gttcagctcc
    25921 tcatagggct tgttaagtga tttggcatga aggcgtgcta ttgccttctg gtttagggcg
    25981 tgaaaaatgg ataccctttt aaagaggggg tgccccgaaa tacgagccac atcgttaagc
    26041 tcatcaacaa caacagggtc ggcaataaat gctttagcat ttggaagcga ttgggctata
    26101 tcatcggcga ttagtgcgcc taagttgctt gcatgctcac ccatgataca gctgcgcaaa
    26161 tcggatttca tctgctcgtt tacttcataa acccctgatt ctattggttt aagcagtcca
    26221 ccgcggccta ctactgcgga tatcttatta acgtcaattc cagtttggcc taattcttcg
    26281 agtataattt tctttcggta gtcaaactgg tctgtaatag ttttgaattg agccaaatcc
    26341 tctaccgagt gcttgatgct cttgagaaag atgctcttgt tgttgttgta aacagcaatt
    26401 tttgttgagg ttgagccggg gttaatggct aaaatcttaa attcatccat tgcacaagtt
    26461 aatttggtag tggggaacta cttaactgca ttagcagcaa gggcaatcga gtacattttc
    26521 gacttaatgc tatcgccacg tgaagtaagg acgcatggaa cttttgcccc cataaccata
    26581 gctccaagct caccattagc aagttttgtg cacgatttga agaaaacatt gcctgcctca
    26641 atgttgggga aaagaataca atcggcatca cctgcaactc cgccacccag tttttttatt
    26701 tgagccgact ccatatcaat ggcaacatca agcgccaatg ggccatccac aatagcaccc
    26761 ttaatttgtc ccctatcggc cattttggag ataatggcag catcaacgca tgccggcata
    26821 ccggcaagca tttgctcggt agcagcattt aaagctacct tgggtttttc aattccaagg
    26881 gaatgggcag tttgaataag gtagtttact atggctattt tctggttcag gtcgggagct
    26941 ggaataatgg caacatcgcc aacaatgagc agcttgggat aagtaggaac ctccacaacg
    27001 gtaacatggc ttaatacagc cttgggtggg agcaatccat tttccttgtc gagtatagcg
    27061 cgcatatact tatcggtgct aaccagtcct ttcatcagca catcgccttc ccccctgttt
    27121 ataagttcta ctgccctacg cgcagccttc atttcgtcgg cttcctgaac aatacggaac
    27181 ttgcccggat ctatgttgtg ctcagcgcac acctttttca taacaggctc atcgcaaaca
    27241 agagtggcat caacaatacc cctatcaaca gccaggctaa ctgcctcaat agtgtgggca
    27301 tcgttggcaa atgcagcaac aagtcttctt cggggctttg attttaccaa atcgaaaagt
    27361 tggtcaagct tatctactct catattccta atctcctcta tagttaaaat taattgttct
    27421 agtttttcaa gttattgata atatttgcag tatcagttgc aatcacaagc tcttcgttgg
    27481 ttgtaatagc cataaccttt acaagggagt taggttttgt aagtagctta tcctgtccac
    27541 gaacgcccct atttgcctct acatcaaatt caacccccat aaaatcaagg gcaccgcata
    27601 ccttttcgcg aagagtgctg tcattttcac caattcctcc cgtaaagatg attaaatcaa
    27661 caccatccat tgctgctgca tacgagccta tgtacttttt taccctgtag tagtacatat
    27721 ccagcgccag gcgtgcacgt tcgtttccgc ttgcagccgc atcctcaagg tcgcgcatgt
    27781 cggaggatat tccggaaatt ccaaaaacgc cgctcttttt attgattaca tcattaacct
    27841 gctgaatgga aagattttcc ttttcggcaa taaacagcaa gatgcccgga tcaatatcgc
    27901 cgcttcgtgt ccccattatt aagccttcca caggagtaaa tcccattgag gtatcaatag
    27961 atttgccatt cttgatggct gcaattgaag caccattccc caaatggcat gtaataattc
    28021 gtagctggtc aaagtcggct cctaaaattt tacaagcttt ttttgcaaca aacatgtggc
    28081 tggtgccatg aaatccatac ctgcgaattt tatagttttc atagtactcg tagggaagag
    28141 cgtaaaggta ggcttccttg ggcatggttt ggtgaaagga tgtatcaaac acagcaacct
    28201 gaggcacatt tggcaaaagc ttctccattg aaagaatacc ctttaagttt gcagggttgt
    28261 gcaggggagc caactcaata cacttctcta tctccctttt cgtgttacta tctatcaggc
    28321 agctcttggt aaaaaactca ccaccatgag ccaccctgtg gccaaccgct tttatctgac
    28381 ttaagctttc taccactcca tgatttttgt caacaagtgc ctctaaaatg agattaatgc
    28441 cggttgtatg atccggaatg gaagccacca tttcatactt atcctttcca actggtttgt
    28501 gggtaagaat ggcatcctta aaaccaacac gttctacaat acccttagct aacagctggc
    28561 tttcgttagc catgttaatc agctggtact tgattgacga acttccgcag ttcaaaacta
    28621 aaataatcat ttttgttatt atttggtaat cagttagttg aaatttgtaa taaaactatg
    28681 caaaattaga aataaaactt atggatttca acgtgtttta agtaatttaa gaaaccttac
    28741 gacctacaaa tagctgagct actgtttaaa aaatgctgta acatggctat ttgcgacaca
    28801 agaacagtaa aatgttctac aacatgctaa aatgaccaat aaaatatgtt gaataaaaat
    28861 cgtataggca aaaatttttc agctaggttt gcaatgcctt aaaagaatgt agatgggcat
    28921 acatatgatt gaagattttg aaaagctggc tacccggctt aaaggtgaaa tacgatggga
    28981 tacccctacc ctgcttcagt atgcaacaga tgcctcagct tacagggaga ttccaaaagc
    29041 ggtggtgtgg cctaaaggca aaagcgattt gcaggagctt gtttcttttg cccaacagca
    29101 aaacctaacg ctaacacccc gaactgctgg cacttcgcta gccggacaag ttgtgagcaa
    29161 tggtgtaatt gttgatctgt cgaagtactg gggaaagatt atcgaagtaa atcagcaaga
    29221 acactacgtt tgggttgaac caggagtaat tcttgatgag ctaaatctat acttaaaacc
    29281 ctacggtttg ttttttgctc ccgaaacctc tacttctaac cgttgtatga ttggtggaat
    29341 ggttgggaac aacagctgtg gttctcattc attgatatat gggagtacgc gcgaccacct
    29401 gctttctgtt aggataatgc tcgacgacgg agaagttcat gagtttagca acctaacgta
    29461 tatcgagttt gaagctaaat gcaagctgag caatcgggag ggcgaaattt accggttttt
    29521 tgagcagctg cttggaaaag aaggaaacag aaagcttata gctgaaaatt ctcctgatcc
    29581 ttcacttcgc cgaaggaatt cagggtatgc acttgatcac ctggcaacca ataccttttt
    29641 taatgggagc accaacccct tcaatctggc taaacttgta gccggtagcg agggaacact
    29701 aggcatagta accgaaataa agctgaacct tgttccgcta ccgcccaagg aaaaagcagt
    29761 tgtggcagtt caccttgaga aaagggagca agcctttagg gctaatctca ttgcattgaa
    29821 gtattacccg gctgctgttg agctaatgga caacgtgatt ttgaactgca caaagggaaa
    29881 tattgagcag caaaagaata gatttttcgt tcagggtgag cctggtgcaa tactgatggt
    29941 tgagttttat ggtagtaacc aacaagagat tgaggaagtt gcaaatgaaa tggaaaatga
    30001 gatgcgcctc aacggatatg gatactactt tcccataatt tgggggaacg aaacaacaaa
    30061 agtttgggct ctccgaaaag ccggattagg tgttctttcg aatgttatag gtgatgcaaa
    30121 gccagtatca cttgtagagg atacagccgt tgcggttgaa aagctacctg actacatgga
    30181 ggaatttgca ggtataatgg gcaagtacaa tctcgattgc gtttaccatg cccatattgg
    30241 cacgggcgag ctccacctcc ggcctgttct caacctcaaa gatccaaacg atgtcgaact
    30301 tttccgtaag gttgggctag aagttgcttc cctggttaaa aagtacaggg gatcgttgag
    30361 cggagagcat ggcgatggac gacttagagg ggagtttatc ccgtttatgt atggaaacga
    30421 ggtttaccag atgatgctcc aggtgaagaa aacttttgac cctaacaaca ttttcaacca
    30481 gggcaagatt gttaacactc cacccatgaa taccagcctg cggtatcaac cgggaatacc
    30541 aactcgggaa attgccacaa tttttgattt tagcggtacc caaggtatta ttcgtgccat
    30601 tgaaaaatgt aacgggtccg gtgattgccg taaaacacac cgcatgggtg gaaccatgtg
    30661 ccccagctac atggctacat taaatgaggc aaacaccacc agggcaagag caaacctgct
    30721 tagagagtac ttaaccaaca gccctaaaga caatcctttt gatcacaagg agctgtacga
    30781 agttttagac ctctgcctga tgtgcaaggg ttgtaaaagc gaatgccctt caagtgttga
    30841 catgacaaag cttaaagctg aattccttca gcactggtac gacattcatg gcattccgtt
    30901 ccgaaacaaa atggttgcct atatcaccca aatttatcgt ttgggggcaa tttttcccaa
    30961 atttaccaat ctattgttat ccagcaaggt aacctcattc gctatcaaga acatgttagg
    31021 ttttgctcaa agcagagcac tgcctaaact aagtagtata actctgcgca gatggcaggc
    31081 aaaacagaag aactcaaata aataccctaa cggtatagtt tacctgttgc ccgatgagtt
    31141 tacaaacttc aacgagtcgc atattggagt aaaagcagct gagctgttaa actatcttgg
    31201 ctacgaagta cgtattcccg atatacttga gagtggcaga acctacattt caaaaggcct
    31261 tatcagaaca gccaagcgca tagccaacaa taacattgat aagcttaagg gaataatagg
    31321 tgaagacaca ccgctggtag gcattgagcc ttcagccata cttagcttca gggatgagta
    31381 ccccgacttg gccgacgacc gtaatacaaa ggcttcgaag gagctggcca tgaattccct
    31441 gctattcgag gaattcttta tgcgagaggc gagcaaaggg aagatagcta aagagcactt
    31501 taagcaggga aatttgagaa ttaaacttca tggccattgc cagcaaaaag cggttgcttc
    31561 cacccaggca acaaaagcca tgctatcatt ccctgaaggg tatgaagtaa gcgaaatacc
    31621 aagcggttgc tgcggaatgg ctggttcctt tggatttgaa aaggagcact acgaactttc
    31681 aatgaagata ggcgaactcg tactcttccc ggaaattcgt aagtcttcag aaaccgatgt
    31741 aatagtagca cccgggacta gctgcaggca gcaaatttat gctggaacag gtcgttatgc
    31801 acttcatccc atagaggtga tgtggcaagc tgccaacatt agcagccagc gttcataggc
    31861 taccacccca ctatttaagt gcatccttca ttgcccggcc aatgttggca ggcgattcca
    31921 caacatgaat gccgcattca cgcatagtat tcattttagc ttcggcagta tcggcagttc
    31981 caccaataat tgcaccggca tgccccattt tccttccttt tggcgcagtt tgccccgcaa
    32041 taaagccaac cacaggctta gttccatgaa tttttaccca ttgtgccgcc tcggtttcca
    32101 tgcttcctcc aatttcgccg atcatcacta tgccatgagt gtcagggtcg ttcatgaata
    32161 gcttaattgc atccagggtt gtagtgccga ttatgggatc accgccaata ccaattcctg
    32221 tactttgccc caatcccagc ttggtaagct ggtcaacagc ctcgtaggtt agggtgcccg
    32281 aacgggatac tacaccgata tggccttttt tatgaataaa gcccggcata attccaacct
    32341 ttgcctcctc gggagtaata attcccgggc aattaggacc aattagccgt gtggcaggaa
    32401 aatcttcaag aaagtacttt accctcacca tatcggcaac cggaataccc tcgctaatac
    32461 atacaacaag tttgatacca gctgccgctg cttcaataat gctatcggcc gcaaaagcag
    32521 gaggtacaaa aattattgaa acgtctgctc cggttgtctt tactccatcg tcaacagtgt
    32581 taaataccgg cctgtccaga tgtttttgtc ctcctttccc tggtgtaacc cctcccacca
    32641 cattagtacc atactcaatc atctgggtgg cgtgaaaggt gccctcgcta ccggtgaagc
    32701 cctgaaccag caccctggaa tttttaccta cgagaatact catctttcct catttttagg
    32761 attacaactc ctatcaaaaa ttaccttaac ctgcatgcaa gaagttaaat tcaagtaaaa
    32821 aaattatcga atcaaaccta gccaatttat aggaaaactg caacaatttt actaacaaat
    32881 ctaacctttt atatacatta caaacgttta cgataaaatt tttcagcatt tccgaatagc
    32941 tatcacaaaa tgtttgcgta acttggtaag ggtattttat aaaagggcaa tgcatacaaa
    33001 tcattaaaat ttaactggtt atggctaaag taaacataaa actagagaac cttagtgaaa
    33061 tgttcccaaa cagctggacg gatgagcaaa aagctaaggg taatacgctg ttcttaaaat
    33121 atttgagcga aaaagcccac aagttctacg gtggcaaagt acaaacagta ccaaaagctc
    33181 ctgttcccgg gtttaactgg tttaatgtgt ggtacacccc cggagtatcg aaagtttcca
    33241 ccaccattcg cgacaacaat gatacctctt ttgagcttac caacaggggc aatctggttg
    33301 ctgttgttag cgattctacc cgtgtgctag gcgatggcga ttgcacgcct ccgggtggtc
    33361 tgggggttat ggagggaaag gcatttttga tgaagtatct tggaggagtt gatgctgttg
    33421 cactttgcat cgacagcaag ggtaaagatg ggaaaaatga tcccgagaag attattgatt
    33481 ttgttaaaat gtgccagcat agctttgggg cagttaacct tgaggacata tctcagccaa
    33541 actgctacag agttctcgac gtgcttcgtg aagagtgcat cattccggtt tggcacgatg
    33601 atgcacaagg aacagcatgt gttacgctag caggattact taatgcccta aagcttgcca
    33661 acaaaaaaat gagtaatgcc aaaatggtgc ttttcggtgc cggagcagcc aacaccacca
    33721 ttgcccgttt gattattgcc gatggcggtg atccgcacaa catggttatt tttgactcac
    33781 acggcggctt gcataaaaat agggaggata taaaggctga tcacaggttc taccgtaaat
    33841 gggaactctg tgaacaaaca aacccacaat gcatcaacac catggaagaa gctatgcgaa
    33901 atgccgacgt gcttatttcg gtttctactc caggtcccga caccataaag caggaatggg
    33961 ttaagctaat ggcacccaaa tcggttgttt ttgcttgcgc caatcccgtt ccggaaattt
    34021 acccctatgc tgccaaagaa gcaggcgctt acattgtggc taccggacgc ggggacttcc
    34081 cgaaccaggt gaacaattcg attggttttc ctggaatttt gaaaggggca ttgattgccc
    34141 gcgcatcaaa aattactgat agtatggcta ttgccgcagc tcactcatta gctggcttcg
    34201 ctgaaaaaag gggtatttcc ccggaaaata ttgttccaac aatggatgaa gctgatgttt
    34261 tccctgttga agctgccgat gttgccgttc aagccgtaaa ggaaggcgtt gcccgtaggc
    34321 aggttagctg ggaggaagcc tacagaattg ccaaaaagga tattgaaagc tcgcgctcca
    34381 tgaccaaagc actttacgac aacgggtaca tagccaatcc accagcagaa atgctcgaag
    34441 atgcgctcct gtggactata caacaggttg aggcaaaaaa gtaaaaggat gatgtttttt
    34501 gttcatccct tgattgggtt ggcaaatggg ttttacacgt catttaactc ttcgagcgag
    34561 cactatacta tatacatata ataatgccca aagaaaactc gggttgaggt ttactttttc
    34621 ccaatcatag gggttatagt atttctaaga atctctgagg caattgaaat cttcatcccc
    34681 aaacaaaagc atgacaaaat ggagtacaga aattcctgta acgctgtaat agatgtcaaa
    34741 attaaaccta cagctttttc ctatgctgaa aaattgtcat gattagtaat tggttcagtt
    34801 ttggatattg cgaaaaaaaa aaaatcaaca tgacaaaacg tgaaaaatat tcgtgggtat
    34861 actggctaag cattttagct tttgtaatag ctttacagct gctagtattt cctgataccc
    34921 aagggccaag agaattcact tatagtgaat ttatcgattc tgtcaatgca ggcaaagttg
    34981 agcgaattgt tttttttgag gataagataa ttggtcagct caaaagcggt gaggaaacag
    35041 gtcaaaaatc aataactcag gcgcctactg caccatggag gcttcgcatt cccagtattg
    35101 agcagcaagt aaaaaggcaa tttgtggtga ataggatacc caacatgaat gaagaatacc
    35161 ttataagcat tctcaccgga acaaatgtta gctttaaagg gcatgtagag aataacgttt
    35221 taagaaactt ttttctgaac tggcttctac cgctgcttat ttttatggcc ctatgggggt
    35281 tctttttcaa aaagatgggg tcgaaaaatc caatgcttga aattggtaag aataaagcgc
    35341 aaatacatgc cgaaaagcca aaaaatccta taacattcaa ggatgtagcg ggggttgatg
    35401 aggcagttga tgaggttaga gagctggttg actttctgaa gtaccccgaa aaattcacca
    35461 ggttaggagg gaagttgcct aaaggggtgt tgcttgttgg tcctccaggg accggtaaaa
    35521 ctctcctggc taaagccgta gctggcgaag ccgatgtgcc cttttttagc ttaagtggtt
    35581 cggactttgt tgaaatgttt gttggagttg gcgccgccag agtccgcgat ttgttcaacc
    35641 aggcaaaagt tcatgctcct tgtataatat tcattgatga aattgacgct ataggtaaaa
    35701 gcagggctca aaatgctgcg tatagcgggg gatacgatga acgggaaaac accctcaacc
    35761 agctactggt tgaaatggac ggttttgatg ccacatcagg agttataatt attgcagcaa
    35821 ccaaccggcc ggatgttttg gatcctgccc tgctaagacc aggaagattt gaccggcagg
    35881 ttttggttga taaacccgac atgaaaggta gggaagccat ttttaaggtt cattccagga
    35941 accttaaact cagctcaaaa gttgatttat cgaggcttgc tgctcaaaca cccggatttg
    36001 caggtgcaga gattgccaac gtatgtaatg aagcagccat tcttgcggta aggaacaata
    36061 gagatgaagt aaccatgagt gattttgagg cggcaataga gcgggttgtt gctggcttag
    36121 aaaagaaaaa taagctcatt aacgaaaagg aaaggcagat tgtagccttc cacgaaagcg
    36181 gtcatgcagt tgtagggtat tttacccctg gtgcggagga ggttcaaaag gtgtctattg
    36241 taccccgggg aataggcgcg ctgggatata ccctgcagat gcccctggag gatagatacc
    36301 tgatgtcgaa atccgaactg ttgggcaaaa tcaaagggct actgggtgga cgtgccgccg
    36361 aagaggtaat atttggagag gtatcaacag gggcttcaaa cgatctggaa cgagcatcac
    36421 aactcgcccg aagcatgatt attgtttacg gaatgagcag taagctgcca aacatttcgc
    36481 tggtaaacaa gtcgaacccc ggctttctgg gacagccttc cggtattgaa agacggtcag
    36541 aatatgtgga gaaactaata gacgaggagg ttaatgaaat aattaatacc tgttaccacg
    36601 aggcaaaaat gttactaaaa gaaaaaaagc atcttctgga taaaatggcc tccattctac
    36661 taaaacagga ggtgattaat cacgatgaaa tcattagtat tcttggagat agaacaccaa
    36721 tcgaggatta aagcaaatgt ttaccgctta acttctatgg ctgaattttt tcatttattg
    36781 ccgccaacat ccggttgagc tctatcacct caataggcct cagggagcta agagcctcag
    36841 aaagaacatc caaaataatg ctctcggctt taggcttaaa attcgaggct gctttcgtag
    36901 agctaaccag ctccgatcgg ctattatcag ggttgggaag gcgaacaacc aaatcgagac
    36961 gttccataca tgccaaggtt cgtgaaagtg aacccttatc ccttcctgat atttgggcca
    37021 aatgagcctg gctgagtggt tcacttgaaa acaaaagggt aagaatgtac cagtaatcaa
    37081 cggtaaagtc atcaccaaaa gcttccgata gccttcgggt aatctcgcct cttatggcaa
    37141 ccgatgtgcg atgtatgttg aatggcaggt ttaaaagatc ttcattaaaa tcatctctca
    37201 taaacaagtg aatattgtgt tagccaagcc gtgaatagtc cgcttaaatt taataaaata
    37261 ttcacaactt attttccact ccagcattta tcggcattcg taaatttgta acaccattag
    37321 tttaaaccaa taataaggtt ttagctctaa tttaatgcaa gcagcaagta ataaaatgct
    37381 tcacaataaa gtttttatat agcattaacg gtttttacct tactagatgt ttaaccacca
    37441 ttctttaaaa ttgatgaatt tttataggag cttattaacc ccattagtag gtattttgat
    37501 tttatcgagc tgtgttaccc aactaacgcc cggggtttcg aaatcacttg ccgactctcg
    37561 cagtgcaaac ataaaaaatt tggaataccg gttatatttt tcgataccca accggttaag
    37621 ttcacctgtt ccgggccgag ccgagcttaa ctttgaactt atcaaccgga aagatttggt
    37681 tattgatttc atgaccgaag ccaccaacct gcaatcaatt accgtaaaca accggctatt
    37741 gagcataagg gttaaaaacg gccacatcaa tattccatcc aaatacctgt taaccgggca
    37801 aaataaagta aatatagatt ttgtatcgtg ttccccatcg ctcaatcgga aagccgacta
    37861 cctttacacg cttctggttc ccgacagggc atcaactgtt ttcccatgct ttgaccagcc
    37921 cgacctgaag gcagctttca acctaactct tgaaattcct aaggaatggg ttgcagtggc
    37981 aaacgcccca ttaggcaaaa ttattgagtc ggatacatca accgtttatg tttttgaaaa
    38041 aaccaagcca gtaagcacct acctgtttgc ttttgcctgt ggtaagttca aagtagcttc
    38101 gcaaccaacc agatatggcg agatggccat ttactatcgc gacaacgatt ctgccaaggt
    38161 aaatcgtaat attgaagcca tttacgagtt gcacacccaa tcgctgggct ggatggaaaa
    38221 ctatacggcc attctatatc cattcagcaa gctggacatc gtgcttattc ccgattttca
    38281 gtataatgga atggagcatc ctggagcaat ctactaccgg gaatcaagca tcttgctcga
    38341 aggcgatccg tcagaaaaca agctgcttaa tcgcgcaaac cttattgccc atgaggtctc
    38401 acaccagtgg ttcggggacc tggttaccat gcgatggttc aatgatgttt ggctaaagga
    38461 ggtattcgca ggcttgatgg ccgataaaat tgtcaatccc cagtttcccg agttcaacca
    38521 ccagcttagc tttctgctat ctcactaccc acgtgcttat tcagttgaca gaacggctgg
    38581 agctaaccct atacggcagg aacttgacaa cctgcttaac gccggctcgc tttatggcga
    38641 tatcatttac cataaagctc ccattgccct tatgcagctt gaaatggcta tgggtgttag
    38701 tgcttttcag gagggattaa gaaagtatct gaatacctat agcatgggaa acgccagctg
    38761 ggatgagctg atagacattc ttgacccgtt aacctcacta aatttaagtg tatggagtaa
    38821 ggcttggatt gaaagtgttg gtatgccaac ccttaaagca acgctaaacg atagcatgta
    38881 cgtagtaaaa aaaatcggcg tacagcttcc aatggtttac agcgtggagg ttttatgcgg
    38941 tagcaatgta aacgaggcta aacccattca gcatattcaa cctgaagttt ctacactatt
    39001 tgcaagtagc tgtgagctag aaagagtgat tgttccaaat gctgatgggc ttggttacgg
    39061 gatgtttgtt cccgattcgc agtcgcttaa actactgtgt gatagttctt ttcgaatagc
    39121 tgatccggtt acccgttcag catccttgat atcggctaac gagctatttt taaacggcaa
    39181 acttgatgga aatacctact tcaacatgct acttgcctca cttgcaaggg aaaacgaacc
    39241 tcaagtgcgc agctacatcc ttgccagtat tgaaacctac tggtggactt ttctggcgga
    39301 tagcgccagg gaaaatttat cgcaacaggt tgaaattcaa ttctccaagt tgttactaaa
    39361 cagttcaatc ccagctgatg agcgaaagtc aattttcaag acatacgcca ggatagctgt
    39421 gtccagcaat gccgctgaag agctgtatgg aatatggagc ggtaataaat ctttaccagg
    39481 tttaaccttc acggagaccg atttaacctt tctagcctac gagctagctg ttagaaatgt
    39541 gcataatgcc gacagtattc tggatctaca ggaaaaaaga atagccaacc ccgacttgct
    39601 tgcaaaattc cgatttgttc gacgcgccct aagtcccgat gacgccttac gcgacgactt
    39661 tttccatagc ttgctgattc ctgaaaaccg acgacctgaa ccctgggtgg ctgaagccct
    39721 tcactacttt aaccatccct taagggatag ttttgccagc caatacctaa gggcttcatt
    39781 ggatttactt cccgagattc agcaaacagg agatatcttc ttccccaaaa cttggcttga
    39841 tgccattcta tggggtaaaa acagcaacca agctgctgaa attgtaagca gctggctaaa
    39901 ccagcaccct aatgtacccg ataacctgaa aaataagctt attcaatcgt cggatttgct
    39961 attcagggct gttgataaaa ggaagaaaaa atagtttcca caaactaacc aaaaaaaagg
    40021 ctactcgctg cccggaggca gaggtagtct tttaaaagga tatgctccta atctgcttac
    40081 ttcacaaact ggagggcata gtactttgcc gttagctttt cgccagtggc cttctcaatc
    40141 atttcgttcc agggatacag gcatcctggc acaaaaacct tttccttgag gtatgcaccc
    40201 acttcggggt gtcccacaaa actagccgca ttaaaatcac taatctttag cacgttggcg
    40261 gctatgtagg cattaagctg tgaggcaagc atttctccca tcaagtagtt atgatagtag
    40321 catgggtaga gggcaacatg aatttttgaa gcccaatcgg gttcgttacg tccttcaggt
    40381 tttttcaaca gctggtactt ttcaaccatg ctccaccaaa gggaattcaa atcctgatcg
    40441 ggattggcgt acatctcctt ttcgaaacgg tacatcacct gagcccagcg gctaaacacg
    40501 agctgctcaa gcctaaggga ttttaggcat tcggaagcaa tttgttcttt ttgttcaggg
    40561 gtgataattc ccatatcgag catccactgg gggttgcttg ccagacgtcc catcatttcg
    40621 gcaattgcct cggttgtaaa gctatgagcg gcatctctta ggaagtaagg ttttgcggga
    40681 tcgttatact tggagtaaac ggcatggcca ttctcatgca aaagggtatt catccaacca
    40741 taggttggct tcacgttgca aagcacccta acatcgcccg aacggtcaat atccgtgcaa
    40801 taggcatgtt gatttttccc gggtttctcg tagagatcgg aacgagctat tatgtcgtca
    40861 atgttaaggc caattccgtt gtagaatttg gctgtaagtg caaccacatc cttgtcgctg
    40921 taaaattggt cgagattaac attgtaaatt ttgggagctt cctgaaagta cctattttgg
    40981 taatgccagg gcatcaactt gtcgggggaa actccaaagc gtttagacaa gtaggagtca
    41041 atttcgagtt taagctcgga aaacgaccca gaggttaagc tgtcgagctc atcaaaaagt
    41101 gccaacactt cgtcgggatc ttgttcagag agaaccatgt tcatggtgtg gtagttggca
    41161 tagcccaatt tttgggcaat ctggttgcgt accttaacca gctcaatgat atcgggggca
    41221 acctcggggc caattttttt gtgcgccttc caggcactct ccacctcgct gctttgggtc
    41281 gaagtggcaa gtatgtcttc tatttcgtta tcggttagct gcttgctgcc aattttagcc
    41341 ctaaagttgg agtacttctt ctcaaggttg gcctccatgg caatttcttt ctcgagtagg
    41401 gtcgggtcaa cctgattacg aagaaaggcc aggtaaagaa tgtccatttg acgagaaagc
    41461 accgaatcct ttaccaatcc ggattcttta atactcttaa gctctgcaaa aagcgaggtg
    41521 tctgcaaaaa ttttaaccag ctcaacctgg gctttctccg atcgctggta atcttcatcc
    41581 ttacccgata tggaggcatc ccacgaagct atagcagctt ctttggataa gggaattacc
    41641 ttcgactcaa cttttgcaat aaagtcggcc atttgttttt ccattttttt ctgattagat
    41701 gtgcatgatg aggcagctac aagcacaatc atcagcatca ctggcataat aggttttatt
    41761 ttattcataa ggcaataatt ttgttgactc aaaattagta aacctttaaa cgatatataa
    41821 ccacaatata aaattatagc gtaaaacttg atgtgtgaac cggcagccta tcagcaaatt
    41881 caaaataata agttggttgt gttgccgaac tataaactcc gtagtaaaat agaagttaat
    41941 ggacaccaac aagaagttag cgggtgcggt cttgtagcta taaaaacatt agctcggctg
    42001 ttaaaggctt ttcagcaaca tgttaagttt ctgaagtgtg gcttcgccaa ccggaacaag
    42061 gggtaaccga aggttgttgt cgataatgcc aagtattgac aatgctgctt taacccctcc
    42121 gggatttccc tcaacgaaaa gagcgtcaat cagctccacc agcttcaggt gaatgacggc
    42181 agcctcagca aacttgcctt tttgagcaaa atgaaccatt tcggcaaaac gtcccggcac
    42241 ggcattgccc acaaccgaaa taataccatc agcacccatg gccatttgtg ataatgccaa
    42301 gccatcgtcg ccggaaatta ccaaaaaatt atcgggtcta tccctcagga tgtacgccat
    42361 ttggttgagg tttcctgaag cttcttttat accgataata tttgggacct cgttggccag
    42421 ggttagcgtg gtttgggcac tcatattaac tcccgttcta cccggaacat tgtagagaat
    42481 caggggtaat ggagagcttt cagccacagc tctgaagtgc atttccaacc ccttctgggt
    42541 tggcttgttg tagtagggcg taaccgaaag aatggcatca acacccttta ggttgaagtg
    42601 ttttatggag ctaatcacgc tcgaggtgca gttaccgcca acacctaaaa caatgggtaa
    42661 cctgccgtta tttttttcaa ctacatgcgc aattaactcg tgcttttcat cgtctgtcag
    42721 ggtcgaggct tcaccggttg tccccatcac aaccaggtag tcaactcccc cgtcaaccaa
    42781 atactccagc aacctatcgg tagcggagta gtcaatagct ttgctgctgc tgaaaggggt
    42841 taccatggca acccctaatc ccttaaatct atcgttttgc ataatagtcg gataagctag
    42901 ttttaagcct gatggggttc atcggcaaaa cggttgttga gcttaaagag aacctgtttt
    42961 acctgctcac agaggtatgg caatccctta agcttgtcaa ccgttaaaat caaatcaaac
    43021 gggttgttga ggtagttttg agagcccagc ttgaatttcg ctttagaaaa tgtcgaaatc
    43081 catcgtactg gaacaacctc atcaaaatct acatcaataa gaatatcata ctccttgtta
    43141 atgaaggctt caacatcgga ggataagggt tttccatacc agttggtttc ggccttacta
    43201 aatatggtta tccctgggtg catggtgaaa acttgtggca cctccttttg ggggtagtag
    43261 cctaaagcgt ggacagttat gtttaaactt tcaaaatatt taaccaggtt taacacgctg
    43321 tcaagaactt cctgactgtt gagggtaaat actatcaaaa tcttacgggc agtagtgagg
    43381 ttatgcacca ttctctgcct cttaaaccct tgagcttgcc tattaatctc gttcagagct
    43441 tttttgtact tataatcgtc aaacatacct tcgcattcta gcttatcaat aataataaaa
    43501 gtgctattaa cccgctactt aattctaaaa ctcggcaaat ttagaaggat aatctaaaag
    43561 gcaattttac aaaaaaatgg gttgataatc aaatttaaca aagctttaca aagtcggagg
    43621 gcaaccgctt gaaaattgac aaccctgaag cttccaatcc caaaatagtt tagtgcagcc
    43681 aggttgttgc ctccccccaa aggtagaatg cgctactctt gaccaataag cttaaaaaac
    43741 tcatcctcgc caactatttt aactcccagc tttgttgcct tttcaagttt agaaggtcca
    43801 atattattgc ctgcaattaa aagggaggtg gaagccgaaa cgctgcttac atttttcccg
    43861 ccgtgctggc ggataagttc ttttaactcg tcgcgcgaaa cttttgaaaa tgttccggaa
    43921 atgactaccg acattccctt aagcgcatcc gaaagctgga ggttttcgat ctgctctact
    43981 tgaagtttta atcccgccat ttttagcttc tcgacaagtt gcctgttagc agggtttgaa
    44041 aaaaattcaa tgatgctttt ggcaattctg tctccaatat cctcaacctc aacaagttcg
    44101 ttgtaggtag ccttggcaat agcctccatg cttccgaaat gttgtgccaa gcttcgtgct
    44161 gtagcttcac ctacgtatcg aattccaatg gcgtaaacaa ccctataaaa gggtacgttt
    44221 ttagattttt cgatacttct aatggcattt tcagcagatt tatgggcaaa acggtcaagc
    44281 aataaaagtt gatccttttt gagaaagtac aggtcagcag catcttttac cagaccattg
    44341 tcgtaaagca aagcaacggt ctcctcccct agtccatcaa tattcatagc cttacggctt
    44401 ataaaatgct ctattctgcc cttaatttgt ggtggacagg caaactggtt ggggcaaaag
    44461 tgtcttgctt catcctctat ttttaccagc ttagaaccgc attcggggca atgggtagga
    44521 aattcgagta ttttggctaa agggctacgt tttgacaggt caactccaac aatcttgggg
    44581 ataatttcac cgcccttctc aacataaacc caatcgccga tgcgaacatc caacagggct
    44641 acctgatcgg cattgtgaag cgaagcacgc ttaacaacag tgccggccag cttaacaggt
    44701 ttaaggttgg ccactggagt aatggcacct gtgcggccaa cctgaaaatc aacagaaagc
    44761 agaacggttg aaacctgctc gggtttgtac ttgtaggcaa tggcccagcg tggggttttg
    44821 gcagtcattc caagtatagc ctgatggcgg tagctgttaa cctttaccac caccccatcg
    44881 gtatcaaagg gcagctgctt acgcgccaaa tcccaatact ttatgaaatc gaatacacac
    44941 tccagattcg aacaaagctg tatatgctcg gaaattttaa acccccactc ctttgcagcc
    45001 atcaagttct ggtaatggtt atcaaaaggc aactcttccc caagcatata gtaaaaaaaa
    45061 ctgtcgagcc tgcgcttggc aacagctgaa gggttgagaa gctttagggt gcctgcagca
    45121 gcattccggg gattggcaaa aggctgctct ccctcatcct cccgctcacg gttaagctca
    45181 tcaaaaatag ctcgcggcat aatgatctca cccctaattt caaactccct cgggtaaccc
    45241 gaaccatgaa gtttcaaggg tatggttcta atggtgcgag cgttggcagt aacatcatca
    45301 cctgcagaac catcgccgcg ggtaacagcc tgcaccagcc tgccatcgag gtaggtaagg
    45361 cctattgcca cgccatcaaa ttttagctcg caaacgtagg ttgggggttc cgaaagggca
    45421 ttacgcaccc ggttgtcaaa atcgcgcaac tcgccttcag aataggtatt tgccaacgac
    45481 agcatgggat agcgatgttg tacctgctta aactcagcac tcgaatcgct tcccacccta
    45541 cgcgagggcg agttgacatc atcaaactcg ggatactgct tttcgagctc caccagctcg
    45601 tttaacatca tatcaaagtc aaaatcgctg atttccggag cagttttcac gtagtagagg
    45661 tagttgtggc ggttaagctc atcacgcaaa aacatgatgc gctgttgcac cctatctttt
    45721 tcttctgctg tcattggttt gtctttaagc ttatgacgtt aacgctaatt atttttcaaa
    45781 tatacttgag aaattaaaat tatgaacagg gaaagttagg ctagcgctgt aaaacacctc
    45841 aggttgaata tacgaaaatt aagtatttgc aaatttatat atttacgggg tatgaacaca
    45901 tgctaattaa acatcttaca agttgaaaac caatgccata gcagataagt acagggactt
    45961 actccgggtt ttacggggta gctgtggaaa ggatgagctg aaaaaaatta ggaaagcgct
    46021 ggatttggtt gtcagcttaa actcggccag ctcaactata cttcacctgc ttgaggttgc
    46081 ccggatttgt gttggcgaaa ttgggcttgg agcccaggct gccattgctg ctatacttca
    46141 cgaaacacta gaaaacaagc aggtaacaag ggttgaaata gagaagcatt ttgattcgaa
    46201 agtagccgag attgttgagg gttacacgct gatatccgga cttgacccaa aggatcctaa
    46261 aactcaggca gagctgttca ggcaaatgct gataacccta tcggggaatg ctgtggtaat
    46321 tctcatcaag ctggccgacc gccttgaaac catgcgctcg ctcagccgtg cagagccaga
    46381 ggtgaggcaa aagcgctcgt gggaaacatt tcacctgtat gctccacttg cccataggct
    46441 tgggctttac cccatcaaat cggagatgga agacctggct atgaagcaca tctaccccaa
    46501 ggagtacaag ttcatagtta agaaacttag ggaaaccact tcaagccgaa acaggtttat
    46561 ccgtagcttt attgagccaa ttgagcagga gctcaaaaat cgccatttcg atttcgagat
    46621 aaagggtagg ccaaagtcgg tgtactccat attccgcaag atgcaaaagc aggatgttga
    46681 ttttgaggat gtttatgatg tttttgccat taggataatt cttaacaccg accccgagca
    46741 tgaaaagtcg gactgctggc aggtttactc catcatcacc gacaagtaca cccccaatcc
    46801 cgaacggctt cgtgattgga tttcagcgcc aaagagcaac ggctacgaaa gtcttcacac
    46861 aacggtgctt ggccccgagg gcaagtggat agaggttcag attcgcacct cacgaatgga
    46921 cgaaatagcc gaaaagggat ttgcagccca ctggaagtat aaggggataa aacaggagca
    46981 gggggttgac gaatgggtaa gtatggttag gaatatactc gaatcgcccg atggttcttt
    47041 tggtgataaa actgacctgc tgcagctcaa ccttaacagc aaggatatat ttgtatttac
    47101 ccccaagggg gaaataagaa aattacccag caaagccacc gttctggact ttgcctttga
    47161 catccactcc gaagtggggt cgaaatgcac cggtgccatt gtgaatggca aaaatgctcc
    47221 aatcaagcaa gtgttgcaga atggcgatat tgttgaagtg ctcacctcaa agaaacaaaa
    47281 gcccaaaaaa gactggctca actttgtaac cacctcaaaa gcaaaaaccc gaattaaaca
    47341 gcagctccgg gaggttgaaa atcaggaaat tgccaggggt agggagatgc tgcttcgccg
    47401 gcttaaaaac tggaaagttg acaaccccga agaagccatt cgcaacatca acaagcagct
    47461 aaaactaaaa gacccaacag aaattttcgt tctgatagcc gaaggtaagg taaaccttac
    47521 ctccattaaa gatatactca cctcaaacat tgaacccgaa gcccctaaga atgttgatgc
    47581 cactactgca cctgcccgcc ctaccgacac aaccggcagc tcatcggaat ttctgataat
    47641 tgacgagcga ctggtaaaca ttgactacaa gctggccaaa tgctgcaatc cgatttttgg
    47701 ggatgagata ttcggtttcg ttacggtgaa ggacggcatc aaaattcacc gtcaaagctg
    47761 cccgaatgcc acccagctga atacccggtg gggataccgg atagttaaag ccagatggag
    47821 aggttcttca aatgatgggg cattccagac ctccatcatg gttacaggta tggacgagct
    47881 ggggatgcta aaccggatat ccgagattat cagcaaggat ttgagggtaa acatgcgcaa
    47941 cctcaacgtc aactccaaaa acggacagtt tgaagctacc atccagctct acgttcagga
    48001 taaaaagcat ctcgaaatgc tgctttaccg gcttggagcc attccagggg taagtaaggc
    48061 cgaaaggcta aggtagcaaa gctgccgagt tgcaattgct taaagttagc taatttggag
    48121 cggttgcatt tcaactctcc catactttta gtatatttgg atgttcataa attggtaagg
    48181 ctatgagcag aaaatcgttg attctattta ccctactaag cctgtttggg atggcagcaa
    48241 ggggacagct ggttcaggat ctatataaaa tctacgaccc tgttttcggt aacgacgacc
    48301 atgactctac agcaatttta actccttttg gagttataaa acccggtgta agctacaacc
    48361 tgtcagttgg cacctcctac tcttcgtttg gcagcgggat gggactatct gaaacctaca
    48421 tatcgcctag ggtgagctac tcacccaacc aaaagttgca ggttgtagcc ggaatttcct
    48481 ttagccgaac gggaacgcac gggttgtcaa caccctcccc caccaacatc agcaccggtc
    48541 aagcccttga caatccatac caggcatggg cctacactca gtataacttc aacaaccgat
    48601 ttagcgttta cgctatgggc tcggttagcc agaatcaaac ctacttctcc ccatattccc
    48661 gaacgctggg gagctacaac agccagatgt acggtgttgg ctttaactac cgcataagta
    48721 gtaaggcaag cattggtgcc agcttcaact tcgttaaccg gaaccccttt ggctaccagc
    48781 tcggtggata tagcaacccc tttattccct actagaaaaa ttaacggtta aagaagccga
    48841 aacatagggc aaaacaattt tccccaggtt gctactatcc taagtattca tcattctaag
    48901 accagtcgtt ccgaacacct gctctaagct ggtccactat tggtttatcc ggttgctaca
    48961 tggccataaa cgtggtcaag ctacagatgc ttacacccaa gcgagccaga aaagtttgca
    49021 ggaaattaga aacctatttg attagttata acatgtacac tacatttgtg tacatacaac
    49081 ttttatagta ctgataccat taaaattggt gtttttatac gacgatgtag tattgatatg
    49141 taagtt
//
LOCUS       DAOSHM010000015        46122 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_617_length_46122_cov_19.285953, whole genome shotgun sequence.
ACCESSION   DAOSHM010000015 DAOSHM010000000
VERSION     DAOSHM010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 46122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 46122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46122
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_617_length_46122_cov_19.285953"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(13..561)
                     /locus_tag="PK839_03885"
     CDS             complement(13..561)
                     /locus_tag="PK839_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG71910.1"
                     /translation="MSSTSEREALVKAFEKRLSEASDEAIIGMLEEIRESGESYMIPS
                     LVNLLFATRGEALKQAVMNFMVDVKIQAAAEYFVSAIQNNPSSPDLYRLVSVCWQSRL
                     DFSSYIDLFLTLLCNADLQTSIEAFSVIENSLDAKSDEEVKELTSRARSMVEHAAEPN
                     KPLVAQLVKLLENFQRDEWQQL"
     gene            complement(897..1721)
                     /locus_tag="PK839_03890"
     CDS             complement(897..1721)
                     /locus_tag="PK839_03890"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF019887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOG71911.1"
                     /translation="MSIRLVATDLDGTLMRSNGEFSVEDIGTLCRLGKLGVVRVIATG
                     RSPYSASRVLSPDFPIDYLVFSSGAGILKWDTKTLVHTNEMPAALVQTIISWLCFHGI
                     DFMVHNPIPLNHYFQYFTAGNSNPDFERRLNLYKEFATPLIQGVPYPNPASQVVAILP
                     NDEGMFNSLRSGVEGVKIIRATSPIDGSSIWMEIFPETVSKASGIRWICQNLEGVTPD
                     QIVVVGNDYNDSDMLQLTAHSFVVENAPELLKKSYTVVPSNDSSGFSYAINKVLGM"
     gene            1868..2128
                     /locus_tag="PK839_03895"
     CDS             1868..2128
                     /locus_tag="PK839_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HOG71912.1"
                     /translation="MTKIITFNELRRVKDNLPAGSMQVIADRLNINVETVRNYFGGTN
                     YKDGAAVGFHVEQGPDGGLVTLDDTTILDIAYEILGNPRELV"
     gene            2212..3063
                     /locus_tag="PK839_03900"
     CDS             2212..3063
                     /locus_tag="PK839_03900"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOG71913.1"
                     /translation="MDFDFNVQKIEEAYRHELLSYLNQLFTGVNLPSHDISHHERVWR
                     YCRSLLLEINRFGLDVPADLVENAIVACYFHDTGLTINLGESHGALGAEICSRYLQQK
                     PNFTSFRNKEILTAIEFHDDKSIRTEENGDALSMLNLTRLVSTADDLDAFGTIGVFRY
                     IEIYLKRAVAANELPGRVLTNLQNRYSNFKSAYALLEKFVDRQECRYYQTFNFFTRLA
                     TEVTLGVGSANGPYGVYRVIKNNLVEKGQSIADVIDYVNENPISEYAQSFFNVLKVEL
                     NINSTVS"
     gene            3067..4347
                     /locus_tag="PK839_03905"
     CDS             3067..4347
                     /locus_tag="PK839_03905"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacetone oxidase family FAD-binding enzyme"
                     /protein_id="HOG71914.1"
                     /translation="MQPVQKFDVVVIGGGPAGLLAGGRAGQLGMKVAILEKMEKPARK
                     LRITGKGRCNITNLKSYDDFVGEIVPNSHFLAKAFGNFFSSDIINLLESNGVPTVTER
                     GQRVFPASGKAWDVAEALVGWAKRCGAQLFNHYEVESLLVSGSAITGLKVRVNKLSES
                     IVFSCKAIVVATGGKSYPATGSTGDGYRFAQQVGHTLTDLRPALTGIETHPAFSFTGR
                     LTLKNVQTTLWVDGAVAGEEFGEVELADYGLSGPAILKLSLKSVDAFRSNRKVRITID
                     LKPALTEEKLEARIIRDTSRSGYFSLEMLLRGLLPPPLVPVYLRLMNLKPKLQAALLN
                     EAERKKLVGHLKNLSFEMTGYRGWNEAIVTAGGVSLNEVDDSTMASKLVQGLFLAGEV
                     LDLTGNTGGYNLQIAFSTGWLAGQSAAAFVQKNA"
     gene            complement(4417..5028)
                     /locus_tag="PK839_03910"
     CDS             complement(4417..5028)
                     /locus_tag="PK839_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007842179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HOG71915.1"
                     /translation="MKIICIGRNYMEHAHEMNSEVPEKPVFFMKPDTSLLRNNQPFFY
                     PDFTQELSYEVEVVLRVNRLGRNINRRFAHRYYDAATLGIDFTARDLQRECKQKGLPW
                     EVAKAFDYSAPVGDFIPKKELADINHLPFRLEINDRLVQAGNTSEMIFSFDELIEHVS
                     KFVTFRTGDLIFTGTPSGVGQVKIGDRLKAYIEDRLILDFFVR"
     gene            complement(5415..7055)
                     /gene="pruA"
                     /locus_tag="PK839_03915"
     CDS             complement(5415..7055)
                     /gene="pruA"
                     /locus_tag="PK839_03915"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018361219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HOG71916.1"
                     /translation="MNNAIFKYDFPKNEPILGYLPGSPERLALEAEIKKLSSEVMDIP
                     LIIGGKEVRTGKTGKVVMPHDHQHVLANYHIAGEEEVKMAIEAALKAHEYWGTVSWVE
                     RLSITVKAAELLSTKYRALINAATMLGQDKNVYQAEIDSACEAIDFLRFNAYYISEVY
                     NHQPSSDKSSINRLEYRPLEGFVFAVTPFNFTAIASNLNMSPVLMGNTVVWKPATTAI
                     LSNYILMKVYKEAGLPDGVINFIPGKGSAIGNIVLNHPMLAGIHFTGSTATFNQFWKT
                     VSNNIGHYRSYPKLIGETGGKDFMFLHSSANINEAVTAAVRGAFEFQGQKCSAASRAY
                     IPQSLWPEFKSKMTATLKEIKQGDPTDYETFINAVIDEASFDNIMQYINQAKTSDKAE
                     IIWGGKGDKSRGYFVEPTVILTTDPHFVTMEEEIFGPVLTIYVYPDNKFNETLEICNS
                     TSPYALTGAIFATDRMALNHACRVLRYAAGNLYFNDKPTGAVVGQQPFGGARASGTND
                     KAGGPLNLIRWTSPRTIKENLIPPVNYKYPYMTNGDSK"
     gene            7446..8993
                     /locus_tag="PK839_03920"
     CDS             7446..8993
                     /locus_tag="PK839_03920"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HOG71917.1"
                     /translation="MHEKDSKVEELKKRKEQILQGGGEKAVAKQIAMGKLTARERIIA
                     LLDEDSFHEYDMFVEHEARDFGMEGKVLYGDGVIIGTGTIYGAPVAVFAQDFTVAGGS
                     LGSMHARKITKIMDYALRMRIPLIGINDSGGARIQEGVNSLAGYGEIFWRNTLNSGII
                     PQISVILGPCAGGAVYSPALTDFVFVVDNISKMFITGPDVIKSVLGEEISQEDLGGAK
                     VHAQITGNAHFYAESEYECFEQIKKLITFIPWNNTRKARRFEPKDPKACYNIEEIVPG
                     DPKQPYDVRDVIRAIVDDSNFFEIQEMWAGNIVIGFGRMNGETVGFVANQPLVMAGVL
                     DVDSSDKAARFIRYCDAFSIPLCTLVDLPGYLPGIDQEHAGVIRHGAKLLYAYSEATV
                     PKLTVILRKAYGGGYIAMSSRHLRADFVFAWPGAEIAVMGPEGAANIIFRKEISEAAN
                     PEEMRRQKVEEYKEKFANPYVAAAKGYIDTVIEPAETRKLLLHALEVASTKVSDRPFK
                     KHGIPPF"
     gene            9005..9364
                     /locus_tag="PK839_03925"
     CDS             9005..9364
                     /locus_tag="PK839_03925"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HOG71918.1"
                     /translation="MTKIDINGNTHDTPKLNDSQLNTLAVEDGEYKTLFTPKFSGYKK
                     WEQHNEKLIRAFIPGTIVEVFVKPGQPVKQGDMLLILDAMKMNNQVKSPINATVKEVN
                     VKVGDKVSKGVDLVVLE"
     gene            complement(9418..10239)
                     /gene="ispE"
                     /locus_tag="PK839_03930"
     CDS             complement(9418..10239)
                     /gene="ispE"
                     /locus_tag="PK839_03930"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HOG71919.1"
                     /translation="MIVFPNAKINIGLNIVDRRSDGFHNISTFFYPVKGLCDMLEVII
                     AEDQDLPLTFSQTGVETNCPANENLCVRAVGLMSKRLALPPIAMHLHKVIPTGAGLGG
                     GSADGSFTLVAINELLGNPLGKKELMELSLQLGSDCPFFIENQPCLAEGRGEKIRPLK
                     IDLSGYFLLVLKPDIHVETGKAYSETKPAPWGVSLVNLIKDDINTWRLTIKNDFEEII
                     FTRYPQIGEIKKRLYGMGAIYAGLSGSGSAVYGIFDREPKDWVKREGYFIRCVRL"
     gene            10654..11319
                     /locus_tag="PK839_03935"
     CDS             10654..11319
                     /locus_tag="PK839_03935"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HOG71920.1"
                     /translation="MMTKRLVHVMAFLMLTSMGVNAQKFSFGVFVDPQLTWFNSDTKR
                     YDPNGPVAGFNIGFSGEKYFAQRYALSSGLSLNTLGGNIRFMQDTFTLKTNDGEYTVT
                     PQTNVKFRAQYLTLPVSLKFRSTQIGYTSFYANVGFKGSIRLKGFAWYSPQNVDREVA
                     NDHFAWGFVSYFIGLGMEYSLGGESALQCGLSFSDGLTGMLDTSNGKITSQSLTLRLG
                     IIF"
     gene            11358..13028
                     /locus_tag="PK839_03940"
     CDS             11358..13028
                     /locus_tag="PK839_03940"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HOG71921.1"
                     /translation="MRIALAQLNYTINHFDLNRGKIVDAIERAKSEGADLVVFSELAV
                     CGYPPRDLLEQEEFIQKCYSSINAIASCCSGITAIVGAPSLNPNPKGKMLYNSAFVLQ
                     NGVVHSIHGKTLLPDYDIFDEYRYFEPADDGYDLVEVAGKRLAVTVCEDLWDDQPTGE
                     ANFRRHKLYRSSPMEHMAMLKPDIIINIAASPFSYNIEQQRLQVLQGNIVRYGLPVIY
                     VNQVGGNAELLFDGGSMVLDANGAVACRLTSFSEDFRVVSSDDFKHSCRSSECSVENQ
                     ENRIARIHDALVMGISDYFRKMGFSHATLGLSGGIDSAVVLALAHSALGSDAIRVLLL
                     PSQYSSKHSVDDAVTLATRMGIRYDTISIQPIFDSFKSQLADLFQGLPEGVAEENIQA
                     RVRGTLLMAISNKFGHLLLNTTNKSEAAVGYGTLYGDMNGAIAVLGDVYKTDVYNLAS
                     YINRNQEVIPPSIISKPPSAELRPNQKDTDSLPPYDVLDRILFAYIEQNHSEKDIIGM
                     GFDSALVHRVIRMVCTSEYKRFQAPPILRVSSKAFGYGRRMPLVSSWT"
     gene            13239..13949
                     /locus_tag="PK839_03945"
     CDS             13239..13949
                     /locus_tag="PK839_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007765214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOG71922.1"
                     /translation="MSKKLQHQVDEDSVFEGVCGLFPNLKPEEREKMRNNFSCMFVPK
                     GKIIYREGDTPPGLVCLSKGIVKLYKEGVGGRDQIVRMAKPVGFMGYRALFADENYTA
                     TAQALEDSVVSIIERNALMEILRNNAELTLHILKSVASELGFSTNRTVTLTQKHVRGR
                     VAESLIFLRDTYGFEDDGVTLKAYLSREDMANLSNMTTSNAIRTLSSFAEEGIIELDG
                     RRIKILDQYKLERVSNLG"
     gene            complement(13958..16426)
                     /locus_tag="PK839_03950"
     CDS             complement(13958..16426)
                     /locus_tag="PK839_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     UDP-N-acetylmuramoyl-tripeptide:D-alanyl-D-alanine
                     ligase/alanine racemase"
                     /protein_id="HOG71923.1"
                     /translation="MQPYTTGQIAQILNAKLEGDPNLEISTIATDSRTPSKSKGTLFV
                     AIVGKHHNGHKFISELYNFQHIRAFMVSDTLGLAEKFPQATFIVVKNTLHALQKLATY
                     HRAQFKYPVVGITGSNGKTIVKEWLNQLLSPDYSIVRSPKSYNSQIGVPLSVLQMDSN
                     FNLGIFEAGISLPGEMLRLQPIINPQVGIFTNIGLPHQENFEDLDEKITEKLKLFKSS
                     ELLIYCKDHELVDRIIYNDDTLSGVKLLSWGVHPQSTLHVESTEEHGNGRAIRVKYGE
                     ETFSFEIPFTDQASYENAMHCLSLLFYLNIDRTTIADRMAKLSPVAMRLELKKGVNGC
                     TIINDSYNSDLGSLSIALDFLLQQKQHDNRVLILSDILQSGYAPELLYSMVAQLVEEK
                     EISHIIGIGKNISRYAHLFDISKEFYSNTLEFTQSITRRQFSNSAILIKGSRNFHFER
                     IVAILEEKVHRTVMEINLNSMVHNLNHYRNQLNPGVKLMVLVKAFSYGSGSYEIANLL
                     QFNRVDYLGVAFADEGVSLREAGIALPIIVLNPSYGAYELMIENRLEPEIYNFEGLAE
                     FANALDRMGVNEYNIHLKIDTGMHRLGFTGEQLPELTSTIQSLKSIRIASIFSHLAAS
                     EDSGQDEFTQSQIALFSTISDEIISAIGYKPLRHILNSAGIERFPHAQFDMVRLGIGL
                     HGISPTQQDKLMPVSTLKSKVIQVKHLQPGETVGYNRMGKINKPTTVAAIPIGYADGL
                     NRKLGNGIGKMMINGVLVPTIGNISMDTCTLDATGVDVKVGDDVVIFGSNPTIMEVSQ
                     ALNTIPYEVLTSVSQRVKRIYFQE"
     gene            complement(16521..17597)
                     /locus_tag="PK839_03955"
     CDS             complement(16521..17597)
                     /locus_tag="PK839_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOG71924.1"
                     /translation="MKILDWYIIRKFIGTYIYAIILIVGIAVIFDFSEKIDDFMEKGA
                     PFNEIIFRYYLNFIPYFANLFSSLFVFIAVIFFTSNMAYNTEIIAILSSGVSFLRMMV
                     PYFISATIIAIFSLVLTNFIIPPANAVRIDFENKYVKKAYVSSERNIHRQVRPGIFVY
                     MRSFNSYSNIAYNLSIEHFDNNRLKSKLIADYAQWDSTKQTWTIKNYYIRDYLERGEK
                     ITRGQSIDSTLYLTPADFKRRSNVIETMNLFQLNDFIDEQRLQGADNIEALLIEKHSR
                     FANPFSAFILTLIGVSLSSRKVRGGIGLHIGFGLALSFSYILFMRFSTIFAVSGHLSP
                     FIAVWIPNIIFAAIALGLYRLAPK"
     gene            complement(17760..18890)
                     /gene="tgt"
                     /locus_tag="PK839_03960"
     CDS             complement(17760..18890)
                     /gene="tgt"
                     /locus_tag="PK839_03960"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013616618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOG71925.1"
                     /translation="MDFRLIGTDSQTSARAGEIATDHGVIETPIFMPVGTVGSVKGVL
                     HRELENDIDARIILGNTYHLYLRPGLEVLANAGGLHRFSSWSRPILTDSGGYQVFSLA
                     SNRKLNAEGASFRSHIDGSSHFFTPERVMDIQRVIGADIVMAFDECPPGDAEYSYACK
                     SLDLTEQWLKRCVNQFATTEPHYGYSQSLFPIVQGCVYPDLRRRAAEYAVSFNCDGYA
                     IGGLSVGEPAEEMYRMIEVVNYILPKNSPRYLMGVGTPENILEAIALGVDMFDCVLPT
                     RNGRNGMLFTSQGIINLRNEKWKNDFSPIDPLGLTFVDSRYSKAYLRHLFVAKEMLGP
                     QIASIHNLGFYLWLVREARIHIVRGDFLEWKKSMVSRLSQRL"
     gene            19422..20570
                     /locus_tag="PK839_03965"
     CDS             19422..20570
                     /locus_tag="PK839_03965"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOG71926.1"
                     /translation="MKEILSSIETYVLVVGILFIAALVVQLYYYLVVYARVACSKSYK
                     RLLSPEAPKPPVSVVICARNEEENLRHFLPRILEQDYPEFEVVVVNDCSCDESEVLLD
                     RLSKEYPRLQVRTIKEDEKFSHSKKLALTVGIKAARYDWLLLTDADCIPQSDKWIATM
                     ASNFDANAGVVLGYGGYLHQKGWLNKFIRFDTLYIAMNYLGFALMGKPYMGVGRNLAY
                     RKDLFFNNKGFASFVQLDSGDDDLFVSQVATRQNTRVDFRKDAHTRSVPRSSHHLWLQ
                     QKIRHTSTSRYYKPGVKIRLTLEPLSRFLLLASAICLVAFRFFPYWIVALVLFKLSVQ
                     LIIIKSVMVRLGERKILLVSLLWDIYSLYFYPKLLLINRFTRRKTTWR"
     gene            20561..21175
                     /locus_tag="PK839_03970"
     CDS             20561..21175
                     /locus_tag="PK839_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOG71927.1"
                     /translation="MEIAPENLSEKARYDYELVNRALEGDQKSYAELMERYRDAIYFM
                     LLKMVNNKSDAEDLTLEAFGKAFKKIHQYTPNYAFSTWLFKIATNNCIDFIRKRKANI
                     VSIDQNIDDQEGQYISPTSMLSSGNPDPEEKLIKEQNIQLIQDIVSKLKPRYRKLVEL
                     RYFKEYSYEEIADELNLPLGTVKAQLFRARELLSNILNNLPPHH"
     gene            21327..21956
                     /gene="rsmG"
                     /locus_tag="PK839_03975"
     CDS             21327..21956
                     /gene="rsmG"
                     /locus_tag="PK839_03975"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006953214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HOG71928.1"
                     /translation="MVNSSIVTQYFSGLNKDQLEKFERLDDLYGYWNAQINVISRKDI
                     DNLMVHHVLHSLSIARVINFARGTKIMDVGTGGGFPGIPLAIMFPQCQFTLVESVAKK
                     AKVCGEVARSLGLDNVEVLNMRAEALSASFDFVVSRAVATIPQLMEWVEKNIHSGGMN
                     QLPNGLICLKGGSFSDELKPYRKFAQTWRVSNFFDDEYFEEKYIIYIQV"
     gene            22132..22287
                     /gene="rpmH"
                     /locus_tag="PK839_03980"
     CDS             22132..22287
                     /gene="rpmH"
                     /locus_tag="PK839_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005840676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HOG71929.1"
                     /translation="MKRTFQPSNRKRRNKHGFRERMSTHNGRRVLSARRAKGRKKLTV
                     SDEIYHK"
     gene            22418..23542
                     /locus_tag="PK839_03985"
     CDS             22418..23542
                     /locus_tag="PK839_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CofH family radical SAM protein"
                     /protein_id="HOG71930.1"
                     /translation="MQAMLVEREKPLRLTESLGVIVEKVAANQRITVEDAVMLYEDAD
                     LALLSQLANMVCGRINGSNIYFNKNFHIEPTNLCVYSCKFCSYHKAAGHPQSWVLSLE
                     EIRELASRYKGSDVTEAHIVGGVHPKWNIDNYGEIISVVKQVLPEVHVKAFSAIELNY
                     VFRKSGLSPREGFKRLKAHGLNSIPGGGAEIFDSGVRAKICPDKASAEEWLSIHEAAH
                     LEGITSNATILYGHIETYRHRAEHMEAIRNLQDKTHGFSCFIPLKFRSANNPLSYLGE
                     VSLLEDLKNYAVTRIFFDNIDHIKAYWPMLGIENTRLAIAFGADDIDGTIDDTTKIYS
                     MAGAEEQKPAMTVEKLTQLIRESGKVPVERNSLYHAIKYY"
     gene            23699..24568
                     /locus_tag="PK839_03990"
     CDS             23699..24568
                     /locus_tag="PK839_03990"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HOG71931.1"
                     /translation="MENKMTRFFKRISANPYISTILLGIGLFVVLLIVLGISLSIYTR
                     HGQTFAIPDFRGESVDKLPELVRKAGLRMEVTDSVYIFNRAPGSIIDQNPKPGTHVKK
                     NRRVFVTINAKNPIKVEVPDVKGYTLRQAKAILEQQGFEVGKLSFTPDMGVNNVLDQL
                     YKGKPAIPGMLIPKGSKIDLLLGMGMHGERTGLPLLIGVKFEEAKNRIIEASLNVGRI
                     KFDETIETYRDSLEARVYSQYPAYSEAISLSFGTRVDIWLTLNESRIPAIKSFNADSI
                     PAVGSYNGEDIIE"
     gene            24585..26537
                     /locus_tag="PK839_03995"
     CDS             24585..26537
                     /locus_tag="PK839_03995"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOG71932.1"
                     /translation="MKGIRHSSFLAIMTLHVMSVEAQDTLVNLSSYPISNLSSKIQAS
                     SAISKSKSSVQLPFFDDFSRRGVFPTDELWEVTGVTANQLYAVNPPTVGVATFDAVDF
                     TGMLYSYLTTLPTAADTITSLPINLNYLPSDSIYLSFYIQPKGLGYSPSRGDSLVVEF
                     YSPMEGAWVRAWSASVNFADSTLAFRNHLERSSTTTHSNALDRNFFRVMMGITQPEFL
                     SDGFRFRFMNYASISPSQEVQGLKSNCDHWHLDMVYLDKYRNLNDTVINDVAFFKPVR
                     NLLRTYSSVPWKHFTRAFSEEFPSPLEFSVGYRNLGDEVWNVTRRYEIVDESGAQPDY
                     LFMGGAENIHEFQDVSYTRFFSYNFQSAWSDSAKFLLKAYLITENKSPLSLPYYLNFN
                     DTISYNLNFYNYYALDDGTAESGYGLYGEGAENGMVAVRFQNYQADSLSGVMIYFNRT
                     YNNANDIGFKITLWDERNGMPGNIIYQKSGVRPLFTDSLDRFTVYALDKVELKEGSFY
                     IGWQQESSGMLNVGFDMNSNNSSRTLYNLNGEWVETRYNGSVMIRPIFGKLYQIPTNV
                     DIQQPSLGFSIFPNPAQHHIRLSLPDGFTPKAIQLINTAGHVVASRPYETNELFYLEG
                     IAPGFYMVRVSNDMGRCLTGKVIVVK"
     gene            26534..27601
                     /locus_tag="PK839_04000"
     CDS             26534..27601
                     /locus_tag="PK839_04000"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010261640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOG71933.1"
                     /translation="MIDKLHPFFNGEVDEETDEQAELYEHFHFDVDRGQAQIRIDRFL
                     TDKIKNVSRNRIQSAADAGNIVVNGKPVKPSYKVKPLDSISIVMAYPPRDTDIKPENI
                     PLSILYEDDSLLVVDKPAGMVVHPAHGNFSGTLVNALLYHLRDVPLFSSGEVRPGLVH
                     RIDKNTSGILVVAKDEVSMNRLARQFFERTTSRKYIALVWGDMEADEGTIKGNIGRSV
                     RDRKKMQVFTDEEQGKHAVTHWRVVERFGYVTLVECRLETGRTHQIRVHFEYIHHPIF
                     NDEVYGGNKILRGTLHSHYKQFVENCFEICPRHALHAKTLGFTHPLSGMEMNFESPLP
                     NDISALIEKWKKYSTGKYSEK"
     gene            complement(27624..28043)
                     /locus_tag="PK839_04005"
     CDS             complement(27624..28043)
                     /locus_tag="PK839_04005"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HOG71934.1"
                     /translation="MTSSKTSHYKVIFPGDLNNNGTLFGGLAMQWMDEVAYITATRFT
                     RKKVVTVSVCKVNFVAPVQPNMIVEIRGTVQKVGLVKMEVLVEIYQEDMYSDYRNKAV
                     EATFIFAAVDNDNKPIRLMDSALKLKIKCDSANERYL"
     gene            28131..28691
                     /locus_tag="PK839_04010"
     CDS             28131..28691
                     /locus_tag="PK839_04010"
                     /inference="COORDINATES: protein motif:HMM:NF014101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HOG71935.1"
                     /translation="MARLKIPRKITNPPIVKGFKPYGPDIDSKKRESIILNFEEYEAF
                     RMCDYDMYNHYQASVLMNVSRPTFTRIYASARMKISKAFVEGRQIEFEGGKVYFDSDW
                     YQCNSCGCHFNNPEISISIQNCPLCGSSEVERHTEESLGDMPRNSEDDLCICPECGFT
                     KEHHYGVPCRNEKCPTCNSPLIRKYH"
     gene            28703..29026
                     /locus_tag="PK839_04015"
     CDS             28703..29026
                     /locus_tag="PK839_04015"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOG71936.1"
                     /translation="MRIAITSQGNSLESILDQRFGHCKYFVIVDMQTGAIEIIPNPYS
                     EAEEQAGTLAVKLLSSKNVNKIISGEFGIKVKPLLDSLNIQMIMYKKPNIPIGKVIEM
                     LNHNN"
     gene            29031..29222
                     /locus_tag="PK839_04020"
     CDS             29031..29222
                     /locus_tag="PK839_04020"
                     /inference="COORDINATES: protein motif:HMM:NF036294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 family protein"
                     /protein_id="HOG71937.1"
                     /translation="MPKMDGTGPEGKGAETGRKLGDCNQLTDEEKASKLGKGLGKKRH
                     TGGGQGKGKRLKYNEKTNQ"
     gene            29235..29462
                     /locus_tag="PK839_04025"
     CDS             29235..29462
                     /locus_tag="PK839_04025"
                     /inference="COORDINATES: protein motif:HMM:NF036294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 domain-containing protein"
                     /protein_id="HOG71938.1"
                     /translation="MPGFNQTGPMGQGPMTGRRMGRCTNYGANQGHTSNSEGNSQTDV
                     FQGRGLGFGRGQRGRGRGRGFGMQNRSRGGF"
     gene            29614..30000
                     /locus_tag="PK839_04030"
     CDS             29614..30000
                     /locus_tag="PK839_04030"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOG71939.1"
                     /translation="MTRKRFAVPMEGNVLSAHFGHCQAFAFVDVENNNITKVTVLDPP
                     EHQPGTFPKWVAENGATEVIAGGMGPQAVNLFNGYGVNVFVGAPIKGVEELVNDFLAG
                     RLSLNANYCDHGDGSHEHHHHNGNCH"
     gene            29951..30862
                     /locus_tag="PK839_04035"
     CDS             29951..30862
                     /locus_tag="PK839_04035"
                     /inference="COORDINATES: protein motif:HMM:NF014046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ARMT1-like domain-containing protein"
                     /protein_id="HOG71940.1"
                     /translation="MAMAATSITTTMETATKTDINIQSQCYSCFLKTYQRLFEKFMLT
                     QEQQDEFLDFFHNTLEKYKNSTSPEVQRELNQGFSKIVGIEDLFEDEKINSNSIALGL
                     YNDWSTQVPISKNPFDLALKLSIAGNIMDYGANNNFDIKSTIRRVIFAEFAINHSKQL
                     QDKIKEAKSILYLADNAGEILFDKLFIETINHPNITYAVRGGFALNDATLHDAIDVGM
                     NSVARVIENGFDAPATVLAKCSSEFMEVYNSADLVISKGQGNLEGLINENDPRIFFLL
                     MVKCDVMASLLNVAKGSFIVYNPTKNN"
     gene            30864..31307
                     /locus_tag="PK839_04040"
     CDS             30864..31307
                     /locus_tag="PK839_04040"
                     /inference="COORDINATES: protein motif:HMM:NF020400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGGC domain-containing protein"
                     /protein_id="HOG71941.1"
                     /translation="MEKVKIGIIICNRYHTCAGGKCFRALQNREGAFEMYKDKDVELV
                     GYTTCGGCPGGNIEYAPAEMKKNGATHIHLATGLLVGYPPCPRLNHFAKFIPEKYGLK
                     VIFGTHPIPQKYFTTHTNLNTWNSSFYKEAIKDTLTNEATRLQYD"
     gene            31300..32211
                     /locus_tag="PK839_04045"
     CDS             31300..32211
                     /locus_tag="PK839_04045"
                     /inference="COORDINATES: protein motif:HMM:NF025001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG71942.1"
                     /translation="MIKKENLKIAIASGKGGTGKTLLSTNLAAFLSERESTLLVDLDV
                     EEPNDSLFIKGNLIRKTEQHKMIPQWDESKCTLCGECTKNCKFHAVVQMGKFIAVFKE
                     LCHSCYACSELCPAKALPMHPYKMGETAEYSYNNLKLIESRLMIGEEQAVPLIHKTHK
                     LVEENFSNSIFQLFDCPPGTSCPVIAATENADFVILVTEPTPFGLHDLKLAVETMRKL
                     HKPIGVVINRSGIGNDDVENYCKTEQIDILTKIPFDKNIASYYSKGELVYNKLESVKH
                     SLNEILDSLKNKIPCQSLQGFETLKGI"
     gene            32247..33125
                     /locus_tag="PK839_04050"
     CDS             32247..33125
                     /locus_tag="PK839_04050"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG71943.1"
                     /translation="MKEIVVLSGKGGTGKTSITASLAVLAGSEAVVADCDVDAANMHL
                     LMQPDFAVASQFYSGELAEINQNICTQCGLCSNVCRFDAIPFQNNKYTVNPLACEGCG
                     YCQKVCPTEAISMKKRKSGYVYISNTKVNSTMVHARLDIGAENSGKLVAKVKSEAKEL
                     AEHQNKYFVIVDGSPGIGCPVVSSLSGASYVVLVTEPTVSGLHDLERIYSVIKRFRIK
                     AGCIINKYDINPKKSEEIIEFLNRENIVHLASIPYDIAFTEAMTQAKTIVETSSRISQ
                     TLIDAWNKIKEEAAKN"
     gene            33131..33469
                     /locus_tag="PK839_04055"
     CDS             33131..33469
                     /locus_tag="PK839_04055"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOG71944.1"
                     /translation="MRIAFTSTGKSWDSIIDSRFGRTEYIVIFDEETKTLELVDNTAV
                     KNEAHGAGTATAQKMYEVKPDVLITGNGPGETAATALKRLNMKIYVDAHNLTLEQAYE
                     SYRNGALKEI"
     gene            33485..34426
                     /locus_tag="PK839_04060"
     CDS             33485..34426
                     /locus_tag="PK839_04060"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOG71945.1"
                     /translation="MEEFVFGPVPSRRLGKSIGVNNIPHKVCSYSCSYCQVGKGEKIQ
                     IERQEFYNPDTLVKQVEQKLNMTSHAEYPDYITVVPDGEPTLDINLGKLITKLKTFGL
                     PVAVITNSSLIYLDDVKRDLSNADYISFKVDAVNHSIWKYINKPHRKLDLHEILNSLM
                     DFSKYYRGKYVTETMLVKGDNTADIELAATAQFLQQLKPSIAYLSIPTRPPAFENTFP
                     PCEETLLKAYEVFYWHCLNVEYLTGYEGNAFTSSGNFENDLLGITAVHPMRIDAVKEL
                     MAKTKTNDGVLEKLVEENKVKRILYERQTFIVRSFNK"
     gene            34448..35209
                     /locus_tag="PK839_04065"
     CDS             34448..35209
                     /locus_tag="PK839_04065"
                     /inference="COORDINATES: protein motif:HMM:NF025716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4405 domain-containing protein"
                     /protein_id="HOG71946.1"
                     /translation="MIKGIQKSKLGIITDIILLVFLALMAGIGFLMKYVLLPGIQRNS
                     KYGADINLEFLGMDRHQWGTIHLITSKIFITLIILHIILHWDMIICILDKMIPRKLVR
                     SWFLAILAIITLTQFINPFLASPALVSYENNYRNTAERVESEPNGMDPLHKTANVEAK
                     KPIPDQSKTSRHSRAKEEYGVHGIQTINGVSVQFTIPAGFIGKELKISEGNQYQPLVR
                     LRKGYGFTMSEVGNAIAKIQEFSGGTIKHLNHEPK"
     gene            35196..35726
                     /gene="cobO"
                     /locus_tag="PK839_04070"
     CDS             35196..35726
                     /gene="cobO"
                     /locus_tag="PK839_04070"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006745397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HOG71947.1"
                     /translation="MNQSNQQNGYIHLYTGNGKGKTTAAIGLAVRAAGANKTIFIGQF
                     VKGMHYAELVSLQRIPQIKVKQFGLDCFIVNNPTQKDVDAARAGFDEISGIMLNNSYN
                     MVILDEICIAIYYRLIGVNEVIDVLGKKPREMEVVLTGRYAPQELIDIADLVTEMKEI
                     KHYYNQGIEARKGIEY"
     gene            35744..37099
                     /locus_tag="PK839_04075"
     CDS             35744..37099
                     /locus_tag="PK839_04075"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOG71948.1"
                     /translation="MKRTDVLVIGGSAAGMVTATTGKAHWPEKSFTLVKKQKDVMVPC
                     GIPYIFGTLESSEKNIMPVDSMMEKIGVKSLVDEATDIDTKGKVVSFASGETIQYEKL
                     VIATGSNPVKPKWLAGADLENVFVIPKDRVYLDEMKKKLGSVKNIAVIGAGFIGVELS
                     DELVKCGLNVTLIEKLPHILNLAFDVELSSKIHEMLTARGVKVITGVGISKVHGTGKV
                     QSIELEDGRKMEMDAVVLSMGYRPNTELAKKAGIFIDEDNFIAVDEYMRTHTPDVFAV
                     GDCAQKRDFVTRRRVATMLASTACAEARIAGMNLFNLSVVKTFSGTIAMYSTAIGETG
                     FGTAGVTEQRAQQEGLSTLTGIFEGVNRHPGNLPGTHKQIVKLVAASNSGVIVGGEVI
                     GGLEAGELTNVIGLAIQNRMSVNSFLTMQIGTHPCLTASPAAYPLIKAAEQIAGKMIK
                     K"
     gene            37105..37893
                     /locus_tag="PK839_04080"
     CDS             37105..37893
                     /locus_tag="PK839_04080"
                     /inference="COORDINATES: protein motif:HMM:NF022077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HOG71949.1"
                     /translation="MNPIEKIELDGVKNIVVVASGKGGVGKSTVSSNLAISLARNGYK
                     TALVDADIFGPSIPRMFGIEKAQPEVTHLGDKEVMFPIEKYGVKIMSIGFFIKKNQGL
                     IWRGPMAANAIMQLFENTLWNDIDYMIIDFPPGTSDIQLTTVQKLNVTGAIIVTTPQE
                     VALNDARKAASMFNNPDLNVPIWGVIENMAWFTPQPHPDEKYYIFGQGGGEMLAKELN
                     TKLLGQIPLVAEVGDAAEKGLSVYNQSNPTVIEAFEKLTETITA"
     gene            37975..38559
                     /locus_tag="PK839_04085"
     CDS             37975..38559
                     /locus_tag="PK839_04085"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOG71950.1"
                     /translation="MESLKNRILNCTKCDLYKTRNNPIFGEGNIHAPIFIIGEAPGRE
                     EDLVGRPFIGKSGQLLDKILAACNFNRNYHVFISNIVRCRPPENRVPTSREAEICMPY
                     LLEQIELVNPKILVLLGSTALKYMAGADHRITKERGTWLEVYGRLAMPVYHPAALLRD
                     PSLKRDTWEDFKKIFYKYRELVNPEHQSPHISIG"
     gene            38767..39009
                     /locus_tag="PK839_04090"
                     /pseudo
     CDS             38767..39009
                     /locus_tag="PK839_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008616585.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
     gene            complement(39032..39931)
                     /locus_tag="PK839_04095"
     CDS             complement(39032..39931)
                     /locus_tag="PK839_04095"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOG71951.1"
                     /translation="MEFSHTSHSSKGLSSAFKIGIAINVVFISVESAFGFLSNSSALI
                     ADAGHNLSDTLALAFSWIAVVISHRKPTKRFTYGFRRSTILVAMLNTILLLAVVVFIT
                     IEAIDRLRNPSEINTRSVIVIAAVGILVNGLTAWLFIKDKKKDLNIRSTFVHFVADAL
                     LSLGVVAAGVMITFTGFVWIDPLVSLVIVAIILLSAIRLLVDSVSLALDAVPEGINIQ
                     AVSNYLKQLPEVKGIHDLHIWALSTTDVALSVHLCTSKQTDLNFISTIQQQLQKQFNI
                     EHSTIQVEYGVTDSECTSSCHPN"
     gene            complement(39933..40292)
                     /locus_tag="PK839_04100"
     CDS             complement(39933..40292)
                     /locus_tag="PK839_04100"
                     /inference="COORDINATES: protein motif:HMM:NF014672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HOG71952.1"
                     /translation="MQNILIVINESPYGTEKAYNAMRTAMTLQKEHGDELNVKVFLMA
                     DGVFCGLPNQTTPNGYYNIERMLDAVIRQGGEVKSCGSCLSARGMEAVDLIEGVQPSN
                     MKEFTRWIVECDKVLSF"
     gene            40541..41077
                     /locus_tag="PK839_04105"
     CDS             40541..41077
                     /locus_tag="PK839_04105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG71953.1"
                     /translation="MIMKMGIAQNDWCRVALATAFLAFAFFSCKDKPEHFNRSIYEQK
                     SGVAIVADTITYDVQIGVTDTSDLWSMECLKYMNQQVFIDYIFNGIYKGSMVAHDFNN
                     GMIINAEKVRQLEQQPGFSRGKITKIQFKELWYIDSLGIFRKEIISYTLGLEAYSNQN
                     TFLGYSPVFVVKPTNVHP"
     gene            complement(41058..42338)
                     /locus_tag="PK839_04110"
     CDS             complement(41058..42338)
                     /locus_tag="PK839_04110"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HOG71954.1"
                     /translation="MSALRVFKFGGASVKSAEGIRNMAEIIRRQTSGNLVVVVSAIGK
                     TTNALEEILSLYLAKSDEKANKLDQLKVFHTHIATELVGDSSSSHPLLAKVEQLFTEL
                     DEKMKFPPSGGYDYCYDQVVSYGELLSTTMVSFYLNQAGIRNRWIDIRKCLRSDTSYR
                     EGVVDFELSQYLCEKEFCFDSDHLFITQGFIAGTAEGTTTTLGREGSDYTAAVLANLL
                     DATDVTIWKDVDGVLSADPKIFSNTCKLDFISYQEAIELAYCGAQVIHPKTIKPIQNK
                     QIPLYVKSFINPEASGTTIGHSDVPLSLPPVLILKRNQALVSLTPHDFSFVIEESLSK
                     IFAILYRHRVKVNLVQNSAISFTICVDDEHLRLPGAIEELQKTFTVRFNSNLELLTIR
                     HYTSHVVDEMVGERIVYLQQRTRSNARFIMDARL"
     gene            42537..43550
                     /gene="fbp"
                     /locus_tag="PK839_04115"
     CDS             42537..43550
                     /gene="fbp"
                     /locus_tag="PK839_04115"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HOG71955.1"
                     /translation="MNGYNVKTLTQFIIERQADFPYATGEFTRLLYQVGVAAKLVNKK
                     VNKAGLVDVLGEAGNINVQGEEQKKLDLFANWQFIQALRNSRECCGIVSEEDQEIITW
                     DDEFSRDGNYLLCMDPLDGSSNIDVNVSIGTIFSIYRRISPRGEKPTLEDFLQPGNKQ
                     AAAGYVIYGSSTMLVYTTGRGVYGFTLDPSIGEFCLSHFSITTPSDGKIYSINEGNYD
                     HFPEGVKVYIDYCKQQDKPTQRPYSARYIGSLVADFHRNLLKGGIFLYPPTAAAPNGK
                     LRLTYECNPIAFIAEQAGGTATDGSQRILDIKPESLHQRVPFYVGSSNMVGKLQEFID
                     KHE"
     gene            complement(43691..45061)
                     /locus_tag="PK839_04120"
     CDS             complement(43691..45061)
                     /locus_tag="PK839_04120"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008021808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HOG71956.1"
                     /translation="MGRIAVAGATIINEGNRFQGTVIINRGIIEEIITGASTQLTGYN
                     PEHTIDARGLLLIPGVIDEHVHFREPGLTSKGDIYSESKAAAAGGVTSIMDMPNTIPQ
                     TTTLELLDDKISTAAEKSLVNYSFYMGATNDNIEQLVKIDPLKVCGIKLFMGSSTGDM
                     LVSNREALEKIFKLSPTIIVAHCEDEASIRQNAEKIKQEKGENIPFRFHSSIRSAEAC
                     MISSMLAVELAKKHGSRLHILHLSTARELSLMNNSIPLADKKITSEACIPHLWFSERD
                     YDRLGWRIKCNPSIKLESDRQALLEGISSGLVDAVATDHAPHLAFEKDSTYFKSASGM
                     PMVQHSLQVMLELSKKGYFSVEKVVEKMCHAPATIFRIDRRGYIRKGYHADLVLINPT
                     STETVTTQSLHYRCGWSPLEGEIFHNSIETTFINGHIVYHKGKFDETIKGEALSFNQN
                     QLAYQG"
     gene            complement(45144..45896)
                     /locus_tag="PK839_04125"
     CDS             complement(45144..45896)
                     /locus_tag="PK839_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007484944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HOG71957.1"
                     /translation="MPSKEKLVIIPTYNELENIDKMLARVMELEGDFNVLIIDDASPD
                     GTAERVKAIQHNFPGRVSLIERPGKLGLGTAYICGFKWALANGYEYIFEMDADFSHNP
                     DDLVNLYKACRDGADIAIGSRYINGVNVVNWPIGRVLMSYMASKYVRLITGMRIMDAT
                     AGFVCYKGSVLKAINLDKIKLVGYGFQIEMKFTAWKLGYKIVEVPIIFTDRKFGVSKM
                     SGGIFNEALWGVIAMKLKSIFKPYQKLNLPER"
BASE COUNT        13038 a         9972 c        10445 g        12667 t
ORIGIN      
        1 gatatagtca acctataatt gctgccactc atcacgctga aaattttcca acagcttaac
       61 cagctgggca accaatggtt tattgggttc tgctgcgtgt tcaaccattg accttgctct
      121 gctggttagc tcctttacct cttcatccga cttggcatca agtgagtttt cgattacaga
      181 gaaggcttcg atgctggtct gcagatcagc gttacacagt aaggtaagaa ataggtcaat
      241 ataagagctg aaatcaagcc ggctttgcca gcacaccgag accagcctgt acaggtcggg
      301 tgaagagggg ttattttgaa tggcgctcac aaagtactcg gcagcagcct gaattttgac
      361 atcaaccatg aaattcatta cagcctgttt aagcgcttca cccctggtgg caaaaagcag
      421 gttgaccaga gagggaatca tgtagctctc accagattct cttatttctt cgagcatgcc
      481 aattatagcc tcgtccgaag cctcagatag ccttttttca aaagccttta ctagtgcttc
      541 tctctcactg gtgctactca tagctgttct tttaattgca aaattaacaa tgttttacct
      601 tttttatcct tagattttca tgactttttt aaaaccccga aggagcgaaa catactaacc
      661 ctaggcaacg cccggggttt aataaaaacc gaaaatatct gcgatgcaag cagtatctcc
      721 ccaagagccg tttagttcct ttgcatcgta ataggaaaaa atttccttcg aggcatgagt
      781 agatcagagg tatttttctt ccttttaccc ttcaactaat tcagggcatg cttttagctt
      841 cttcccggtt tacgtcgtgc ctctcgatgt ttttgtgata ttgaaatggt tcgtagttac
      901 atgcccaaca ccttgttaat ggcatagcta aaccctgaac tatcgtttga tggaacaacc
      961 gtataggatt tttttagcag ttcaggggcg ttttccacaa caaagctgtg cgctgtaagc
     1021 tggagcatgt cggaatcgtt gtagtcattt cccaccacaa caatttgatc gggagtaaca
     1081 ccttcgaggt tctgacaaat ccacctaatt ccgctagcct tggaaacggt ttcagggaaa
     1141 atctccatcc agattgagga gccatcaatt ggtgaagtag cccgtataat cttcacccct
     1201 tcaactcctg aacgcaagct gttgaacatg ccttcgtcat tgggtagtat ggctacaacc
     1261 tgggatgccg gattggggta cggaactccc tgaattagag gcgtggcgaa ttccttgtaa
     1321 aggttcaagc gtctttcaaa atcggggttg ctgttgcccg cggtgaagta ctggaagtag
     1381 tggttgagcg gaatggggtt atgcaccata aaatcaatgc catgaaaaca aagccatgaa
     1441 ataatggtct gaactagagc cgccggcatt tcattggtat gcacaagggt ctttgtgtcc
     1501 cattttaaaa ttccagctcc tgatgagaaa accaggtagt ctattgggaa atcaggcgaa
     1561 agaaccctgg atgcggagta aggcgaacgg ccggtggcaa ttacccttac aacacccagc
     1621 ttacctaatc ggcatagtgt gccaatatct tcaacgctaa attcaccatt gctccgcatg
     1681 agggtcccgt ccaagtcggt agccactaat cttatactca tgagttttat tttttcccaa
     1741 atataggacg attagcggtt ttttgcagtc tggctaaatt caaaataaaa tgtgttaaat
     1801 aatttttatt ttaggattaa ttgcatatat ttaaaacgtt aaactcatta tctaaaccgc
     1861 tagaattatg acaaaaataa tcacatttaa tgagctcagg agggttaagg ataaccttcc
     1921 agccggcagc atgcaggtta ttgcggacag gttgaacatt aatgttgaaa ctgtccgcaa
     1981 ttattttggc ggcaccaact acaaggatgg agcagcagtt ggatttcacg ttgagcaggg
     2041 ccccgatgga ggactcgtta ctcttgatga taccacaata ctcgacatag cttacgaaat
     2101 tcttggtaac ccgagggagt tggtgtaaac aacattcttt ttgcttcgtt gtaaaaacag
     2161 gatacctttg gcgatatcct gtttttttat acatctggaa ttcttaccca aatggatttt
     2221 gattttaacg ttcagaaaat tgaggaggct taccgccacg agctgctatc ctatcttaac
     2281 cagctattta cgggggtaaa tttaccatcg catgacatat cgcaccatga gagggtttgg
     2341 cgctactgca ggagtttgct tctggaaata aacaggttcg gtcttgatgt ccctgctgat
     2401 ctggtcgaaa atgcaatagt tgcctgctat tttcatgata cgggtttaac catcaatttg
     2461 ggggagagcc acggtgcact tggcgctgag atttgtagca gatatttaca gcaaaaaccc
     2521 aattttacct catttagaaa taaagaaatc ctaacggcaa tcgagtttca cgacgataag
     2581 tcaattagga cagaggaaaa tggcgacgcc ctttcaatgc tcaatcttac gcgcctagta
     2641 tccactgccg atgatctgga tgcttttggg actattggcg ttttcaggta tattgaaatc
     2701 taccttaaaa gagccgtggc cgctaatgag ctgcccggca gggtgttaac gaacttgcaa
     2761 aatcggtatt ccaactttaa aagcgcctac gctttgctcg aaaaattcgt tgataggcag
     2821 gagtgcaggt actatcaaac ctttaacttt tttacccgtt tagctaccga ggtaacccta
     2881 ggtgttggta gcgctaatgg cccgtatgga gtatataggg ttatcaaaaa caaccttgtt
     2941 gaaaagggcc aaagtattgc ggatgttatt gattatgtaa acgaaaatcc tatttcggag
     3001 tatgctcaaa gctttttcaa cgtgctcaag gttgagctca atataaattc aaccgtaagt
     3061 tagacaatgc aacctgttca aaaatttgat gtagtagtaa ttggtggagg ccctgccggt
     3121 ctcctggcgg ggggcagggc cggccaactc ggcatgaagg tggctattct cgagaaaatg
     3181 gaaaagcccg cccgtaagct gagaataacc ggaaaggggc gatgcaatat aaccaacctt
     3241 aaaagctatg acgattttgt tggggaaatt gtccccaact cgcactttct ggcaaaggct
     3301 tttggaaatt ttttcagtag cgatatcatt aacctgctgg aatcgaacgg tgtgccaacc
     3361 gttaccgagc gcggccaacg tgtttttcct gcctcgggaa aagcatggga tgttgcagaa
     3421 gccctggttg gctgggccaa aaggtgtgga gcacagctgt ttaaccacta tgaggttgaa
     3481 agccttttag taagcggtag cgcaataacc gggcttaagg taagggttaa taagctctct
     3541 gagtctatcg tattctcatg taaagcaata gttgtagcta ccggaggtaa atcatatccg
     3601 gcaacgggat caactggcga tggctacagg tttgcccaac aggttggaca taccctaacc
     3661 gacttgaggc cggcgcttac cggcattgaa acccatcctg ctttttcgtt tacaggaaga
     3721 cttactctga agaatgtgca aacaactctt tgggttgacg gggccgtggc tggtgaagaa
     3781 tttggtgaag ttgaacttgc cgattacggc ttaagtggcc ctgccatttt aaaactaagt
     3841 ctgaaatcag ttgatgcatt ccggtcgaat cgaaaggtaa ggattaccat tgatctaaaa
     3901 cctgccctga ccgaggagaa gctggaggca agaatcatta gggatacctc caggtcggga
     3961 tacttcagcc ttgaaatgct gcttaggggt ttgctacctc cacccctggt gcctgtttat
     4021 ttaagattga tgaaccttaa gccgaaacta caagcagcat tgctaaacga agccgagagg
     4081 aaaaagctgg ttgggcatct taaaaatttg tcatttgaaa tgaccgggta cagaggttgg
     4141 aacgaagcta tcgttactgc aggtggagtt tcactaaatg aggttgacga tagcacaatg
     4201 gcctcgaaac tagtccaagg cctttttctt gcgggtgagg ttcttgactt aaccggaaac
     4261 accggcggtt acaacctcca gatagccttt tcaacggggt ggttggctgg gcaatcggcc
     4321 gctgcttttg tccaaaaaaa tgcctgaatg aagtttatcg caggactaca gcttagcatg
     4381 tgattaaagc aacgtaaccc ctcctttgga caatgcctac ctgacaaaga agtcgagtat
     4441 cagcctgtcc tcgatatagg cttttagcct gtcaccaatc tttacttgcc ctactccaga
     4501 aggagtgccg gtgaaaatta aatcgccagt tcggaaggtt acaaatttcg aaacatgctc
     4561 tataagctca tcaaaagaga aaatcatttc tgatgtgttt cctgcctgca ctaggcggtc
     4621 gtttatctca agtctgaacg gtagatggtt aatatcggca agttcctttt tgggtatgaa
     4681 atcaccaacc ggggcggagt agtcaaaagc tttggcaacc tcccacggaa gccctttttg
     4741 tttacactcg cgctgaaggt cgcgagccgt gaaatcgatt ccaagggtgg ctgcatcgta
     4801 gtacctatgt gcaaaacgcc tgttgatgtt acggcccagc cggtttaccc taagcaccac
     4861 ttcaacctcg tagcttagct cctgggtaaa atcaggataa aaaaatggct ggttatttcg
     4921 cagaagactg gtgtcgggtt tcatgaaaaa taccggtttc tcaggaacct cggagttcat
     4981 ctcatgggca tgctccatat agttccgacc aatgcaaata attttcatca gtattaagtg
     5041 tgtatgtagt tattaatcag atcgataagt ttatgcccct cttttattcc ttaataatga
     5101 agagctatct tacactactg acttcatgct aaatttttca tgctctgctg gctttcctct
     5161 tagtggaagt agagtaaaag ccatattgat taattcctcc ttatcgtcaa gattgcacct
     5221 cgaaaaatgc aacaattttt acttacttca aagggtagcc aaaaattgta tttgattgaa
     5281 gcctaagctg gagttgaggg attttattta gtgggcaaat ttcggagtaa aaagctacac
     5341 ttacccttta cttaaaaaat gaaagagcca caatgttacc gtggctcttt ccctcttaca
     5401 gctatgaatg aaaactactt actatccccg ttagtcatat agggatattt gtagttaacc
     5461 ggaggtatta ggttttcctt aatggtacgt gggctagtcc accgaatcag gttgagagga
     5521 cctcctgcct tgtcgttagt tccgctggct cttgctccgc caaatggttg ttggcctacc
     5581 acagcacccg ttggcttatc gttgaaatat aggtttcctg cggcatacct tagcaccctg
     5641 caggcatggt tgagcgccat tctgtcggta gcaaaaattg ctccggttaa tgcgtagggc
     5701 gatgtgctgt tgcagatttc aagagtctcg ttgaatttat tatcggggta aacgtagatg
     5761 gtgagcacgg ggccaaagat ttcctcctcc atggttacaa aatgcgggtc ggttgttagg
     5821 attaccgtag gctcaacaaa atagcctctc gatttgtcgc cttttccacc ccagatgatt
     5881 tcggccttgt cagaagtttt agcctggttg atgtactgca tgatgttgtc aaacgaagct
     5941 tcatcaatca ccgcatttat aaacgtctca taatcggtgg ggtcgccctg cttaatctct
     6001 ttcaaagtgg ctgtcatctt gctcttaaat tccggccata gcgattgggg tatgtaggct
     6061 ctggaagcgg cagagcattt ttgtccctga aattcaaacg ctcctcttac agcagcagta
     6121 accgcttcgt tgatgtttgc gcttgaatgg aggaacataa agtcttttcc gccggtttct
     6181 ccaataagct tagggtagga gcggtagtgg cctatgttgt tgcttacggt tttccaaaac
     6241 tggttaaagg ttgcagttga cccggtgaaa tgaattccgg ccagcatggg gtggttaagc
     6301 actatattgc caattgctga gcccttaccg ggaataaagt tgatgacacc atccggaagt
     6361 ccggcttctt tgtaaacctt catcaggatg tagttcgaga gtatggctgt tgtggcgggt
     6421 ttccatacaa cggtgttgcc catcagcaca ggcgacatgt tcagattgga ggctattgcg
     6481 gtgaagttga acggggttac ggcaaataca aaaccctcca atggtctgta ttccaacctg
     6541 ttgatgctac ttttatccga tgagggctgg tggttgtaaa cctcggaaat gtagtaggca
     6601 ttaaacctca gaaaatcaat agcttcacat gctgagtcaa tttctgcctg gtacacgttt
     6661 ttatcctgcc caagcattgt tgcagcgttg atgagcgctc tgtacttggt tgaaagcagc
     6721 tcggctgctt ttacggttat gctcaacctc tcaacccacg aaaccgttcc ccagtactcg
     6781 tgagctttta gagcagcctc aatggccatc ttaacctctt cctcgccggc aatatggtag
     6841 ttggccagta catgctgatg gtcgtgtggc attaccacct ttccggtttt tcccgtgcga
     6901 acctccttgc ccccaattat caggggtata tccattacct ccgatgacag cttttttatt
     6961 tctgcttcaa gtgccagcct ctctggcgaa ccaggtaggt aacccaggat gggttcattt
     7021 ttcggaaaat cgtacttaaa aatagcgttg ttcatagctt cagttagtta ttttattcgt
     7081 tatgcaaatg tattcatatc gaactaaaaa acaaatgata attatcagga ataagttgaa
     7141 aagtaggtta agaccaattt ttctgaggca gttgttttta cttaaaactc gccaaaaact
     7201 taacaagaat atatccctta tgttcacccg tactattgca tatttcggtt acgttaatgt
     7261 gcttgggcgg taactgaata gtcaagtcct caatgtaaca tcaataaaat cagcatgtta
     7321 atactcttac aggctaaaaa agaagtcaat attcttttat atatggccgc tatttagtaa
     7381 ccttgcactt tcgatgtatg atgaggatta caggtggaca atttactaac caaacaaatc
     7441 aagctatgca tgaaaaagat agcaaagtag aggagctaaa aaaacgcaag gagcaaatcc
     7501 tgcagggtgg cggcgaaaag gctgtagcta agcaaatagc tatggggaag cttactgccc
     7561 gggagcgaat cattgccctg cttgacgagg actcgtttca cgagtatgac atgtttgtag
     7621 agcacgaagc ccgcgatttt ggtatggagg gtaaggttct gtatggcgat ggggttataa
     7681 ttggtacggg aaccatttat ggagcaccgg tggctgtttt tgcgcaggat tttaccgttg
     7741 ccggaggttc gttaggctca atgcatgccc gtaaaatcac caagataatg gactatgcgc
     7801 tccgaatgag gattcccctt attggaatca acgattccgg aggtgctcgc atacaggaag
     7861 gggttaattc actggccgga tacggggaaa ttttttggag gaatactctt aacagcggaa
     7921 ttattcccca gatatcggtc atactcggcc catgtgccgg cggtgcagtt tactcaccgg
     7981 cgctaaccga ctttgtgttt gtggtggata atatttccaa gatgtttatc accggccctg
     8041 atgtcattaa gtcggtgctg ggtgaagaaa tatcgcagga ggatttgggc ggcgcaaagg
     8101 ttcatgccca gattacgggt aatgcccatt tttatgccga aagcgagtat gaatgcttcg
     8161 agcagattaa aaagcttatc accttcattc catggaacaa cacccgcaaa gctcggcgct
     8221 ttgagcctaa ggatcctaaa gcgtgctaca atattgagga gattgtccct ggcgatccaa
     8281 agcaacccta cgatgtgcgc gacgtgattc gggctattgt tgatgattcc aacttcttcg
     8341 agatacagga gatgtgggcg ggtaacattg taattggttt tgggcgaatg aatggcgaaa
     8401 cagtgggctt tgttgccaac cagccgctgg ttatggccgg tgtgcttgat gtggacagct
     8461 ccgataaggc tgcccggttt attcgttact gcgatgcttt tagcataccg ctttgcaccc
     8521 tggttgacct tcctggttac ctccccggga ttgaccagga gcatgcaggg gttattaggc
     8581 atggcgccaa gttgctttac gcctacagcg aggctacagt tcccaagctg acggttattt
     8641 tacgtaaagc gtacggcgga ggatacattg ccatgagttc acgccacctg cgtgcagatt
     8701 ttgtatttgc atggccgggt gctgaaattg ccgttatggg ccccgaaggc gcagccaaca
     8761 tcattttccg caaggagatt tcggaagccg ccaaccccga agaaatgcga cgccagaagg
     8821 ttgaggagta caaggaaaaa tttgccaacc cttacgtggc tgctgctaag ggttacattg
     8881 atacggtgat tgaacccgct gaaacccgca agcttttgct ccatgctctt gaggtggcca
     8941 gcaccaaggt ttccgataga ccatttaaaa aacacggaat tcctccattt taaaaagttg
     9001 gaatatgacg aaaattgata ttaatggtaa tactcacgat acgcctaaat tgaacgattc
     9061 gcagctcaat actttggcgg ttgaggatgg tgagtacaaa accttattca ccccgaagtt
     9121 ttcgggctac aagaagtggg agcagcataa tgagaagctt atccgggctt ttataccggg
     9181 tactattgtt gaggttttcg taaaacccgg gcagcctgtt aagcagggtg atatgctgct
     9241 tattctggat gccatgaaaa tgaacaacca ggttaaatcg cccatcaacg ccaccgttaa
     9301 agaggtgaat gttaaggttg gcgataaggt aagcaagggt gttgatctag ttgttcttga
     9361 gtagctacac ttgtgaagca tgctatatga aaagggcgcc acagcgccct tttttattta
     9421 tagccttaca caacggatga agtagccttc tctttttacc caatcttttg gctctctgtc
     9481 aaaaatgccg tacactgccg atccacttcc cgataacccc gcataaattg cacccatacc
     9541 gtacagcctt ttttttattt cccctatttg ggggtagcgg gtgaaaataa tttcctcaaa
     9601 atcgttttta atggtcaacc gccaggtgtt tatatcgtcc tttataaggt ttacgaggct
     9661 cacgccccat ggggcaggtt tagtttcgga gtacgccttt ccggtttcaa cgtgaatgtc
     9721 gggcttcaaa accagcagga agtatccgga gaggtcaatt ttcaagggtc taattttttc
     9781 gccacgccct tcggcaaggc agggttgatt ttcgataaag aaagggcaat cgctgccaag
     9841 ctgcagggat agctccatca attccttttt ccctaaagga ttgccaagca gctcgttaat
     9901 ggcaacaagc gtaaaggagc catcggccga accacccccc agaccggctc cggtggggat
     9961 aaccttgtgc aggtgcatgg caattggggg taaagccagt cgtttactca ttagtccaac
    10021 cgcccttacg caaaggttct cgttggcagg gcagtttgtt tcaactcctg tttggctgaa
    10081 ggttaagggc aaatcctgat cttcggcaat tatgacctcc agcatatcgc ataaaccctt
    10141 taccggatag aaaaaggtag aaatattatg gaaaccatcg ctacgtcggt caacaatgtt
    10201 gagtccgatg ttgattttag cattggggaa gactatcatt atccattttt atggcaaatt
    10261 tagcaaatgt tcatttcgac acacatcgta aatttgcaac cttggctacc cttaaccttt
    10321 ggttaaactt gaaatggttt gaatgatatt gttgtaggaa tgcagaagta agcatgttaa
    10381 tgcgcaatgc agctgaaaat ggttcctttt cttctatatc actagaacag aaaagtggaa
    10441 catggattac actgatacta ctgacaaaca cgggtaaaaa atctgtgcaa atatgtctct
    10501 tctgctttgt tggagtgtca gtaaaagttt ttcctgcgtg tgagggttaa ttgaaaatta
    10561 tcatgtacgt ttgtatccca ttcttttgta cccgtaagag ttacttcaaa atgatttatg
    10621 ctagactact attctaataa ttaatttttt aggatgatga cgaagaggtt agtgcatgtg
    10681 atggcatttt taatgcttac aagcatgggg gttaatgccc aaaagttcag ctttggcgtt
    10741 tttgttgacc cacagcttac ttggttcaac tccgatacca agcgctacga tcccaatggg
    10801 ccagtagcag ggttcaacat tggcttttcg ggcgagaagt actttgccca gcggtatgcc
    10861 ctatcatctg gactatcgtt gaatactctg ggtggaaaca tcaggtttat gcaggatacc
    10921 ttcaccctga aaaccaatga tggcgaatac accgttactc ctcaaacaaa tgttaagttt
    10981 agggctcagt accttaccct gccggtgagc ttaaagtttc gatctaccca aattggctac
    11041 accagctttt atgcaaatgt tgggtttaag ggcagcatca ggctaaaggg gtttgcatgg
    11101 tacagcccac agaatgttga cagggaggtg gcaaacgacc attttgcttg gggttttgtt
    11161 tcctacttta ttgggctggg tatggagtac tctctaggtg gcgaaagcgc tcttcagtgt
    11221 ggcctatcgt tttccgacgg tctaaccgga atgcttgata catctaatgg taaaattact
    11281 tcccagtcgc ttactttgcg ccttggaata attttttaaa cggttttagg cttgtcgggc
    11341 caaatgaatt ttgcaaaatg agaattgcgc ttgcccagct caactacacc atcaaccatt
    11401 ttgacctgaa cagggggaaa attgttgacg ctatcgaaag ggctaaatca gaaggagcag
    11461 acctggtggt tttttccgaa ctagctgtat gtggctaccc tccgcgcgac ttgctggaac
    11521 aggaggagtt tattcagaag tgctacagct ctattaacgc cattgcatcc tgctgtagcg
    11581 gtataactgc cattgttggc gctcctagcc ttaacccaaa tcctaagggg aagatgctct
    11641 acaactctgc ttttgtgttg cagaatggcg tagttcacag catacatggc aaaacgcttt
    11701 tacccgatta cgacatattt gacgagtaca ggtactttga gcctgcagac gatggctacg
    11761 atctggttga ggttgccgga aaacgcctgg ctgttaccgt gtgtgaggat ttatgggatg
    11821 atcagcctac cggtgaagca aatttcaggc ggcataagct ataccgcagc agccctatgg
    11881 aacacatggc gatgttaaag cccgacatca ttattaacat tgctgcttct ccgttcagct
    11941 acaatatcga gcagcaacgg ttgcaggttt tgcagggaaa tatcgttcgt tacgggttgc
    12001 cggttatata tgtcaaccag gttggtggta acgccgagct gctgtttgac ggcggttcca
    12061 tggtcctcga tgccaatggg gcagtagctt gccgccttac cagtttttct gaggatttta
    12121 gagtggttag ctccgatgat tttaagcact cttgcaggtc atcagaatgc tctgttgaaa
    12181 atcaggaaaa tagaattgcc cgaatacacg atgctctggt gatgggcatt agtgactact
    12241 tccggaaaat gggttttagc catgccacgc tagggctttc cggcggaatt gattcagctg
    12301 ttgtcctggc cttagcgcat agcgccttgg gttccgatgc catcagggtg ctgcttttac
    12361 cctcgcagta ctcatcgaaa cactcggtgg atgacgccgt aacccttgcc accagaatgg
    12421 gaatacggta cgacaccatc agtattcagc ccattttcga ctcgttcaaa agccagcttg
    12481 ccgacctgtt tcagggattg cctgagggtg tagccgagga aaacattcag gcaagggtta
    12541 ggggaacgct gctcatggca atttcgaata agtttggcca tttactttta aatactacca
    12601 ataaaagtga agctgctgtt ggttatggaa cgctttacgg cgatatgaac ggagccattg
    12661 ccgtgctggg tgatgtttac aaaaccgatg tgtacaacct ggcaagctat attaacagaa
    12721 atcaggaggt aatccctcct agcattatct ctaaaccgcc atctgccgag ctacgcccca
    12781 accagaagga taccgattca cttcccccat acgatgtgct cgatcggatt ctttttgcct
    12841 acattgagca gaaccatagc gagaaggaca ttataggcat gggattcgac agcgctctgg
    12901 tgcaccgtgt tattcggatg gtttgcacaa gcgagtacaa gcgatttcag gcgcctccaa
    12961 ttttaagggt ttcgtcgaag gcgtttggct acggacgccg catgccatta gtttcatcgt
    13021 ggacgtaatt accggttgaa agaagcggcc attaatccta cacgctaaac actattattg
    13081 tttttgtttt tcaacttttt attgaatgct tgatattcca tggccaaaaa cttttttact
    13141 ttaaaaggat attatacagc atcgagagcg ctgcctatta aactttgtta aaaacatgtt
    13201 ttttaactat aaaaagccct atttttgaaa aaaaggttat gtctaaaaaa ttgcagcatc
    13261 aagtcgatga agattcggtt tttgagggtg tttgcggttt gttcccaaat ctgaaacccg
    13321 aagagcgcga gaaaatgcgg aacaactttt cctgcatgtt tgtgcctaaa ggtaagatta
    13381 tctacaggga aggcgataca ccgcccggct tggtttgcct ttcaaaaggt attgtgaagc
    13441 tctataagga aggagtaggg ggacgcgatc aaattgtgcg catggccaaa ccggtaggct
    13501 ttatgggcta cagggcttta tttgccgatg aaaactacac cgccactgca caggctctcg
    13561 aagattctgt ggttagcatt atagagcgaa acgcgctgat ggaaattctc agaaacaacg
    13621 ctgagcttac cctgcacatt cttaaatcgg ttgcttccga gctcggattt tccactaacc
    13681 gaacggtaac ccttacgcag aagcatgttc ggggacgcgt ggccgagtcg ctcatcttcc
    13741 ttcgcgatac atacggcttc gaggatgatg gggtaacgct aaaggcatac ctatcgcgcg
    13801 aggatatggc caacctgtcg aacatgacca cctctaacgc cattagaacc ctttcgagtt
    13861 ttgccgagga gggaataatt gagctggatg gccgaagaat taaaattctc gatcagtaca
    13921 agctcgagag agtgagcaat ttaggttagc tagccggtta ctcctgaaag taaatgcgtt
    13981 taaccctttg cgaaacggag gttagcacct cgtaggggat agtgtttagc gcttgggaga
    14041 cttccatgat agtcggattg ctaccaaaaa taactacgtc gtctccaacc ttcacatcaa
    14101 ccccggtggc gtcgagtgtg caggtgtcca tgctgatatt tccaatggtt ggcaccagca
    14161 ctccgttaat catcatcttt cctattccgt tgcccagctt acggtttaac ccatcggcgt
    14221 agccaatagg aatggcagca accgtagtag gtttgtttat cttgcccata cggttatacc
    14281 caactgtttc gcccggttgc agatgcttta cctgtatcac cttgctcttt aacgtgctga
    14341 cgggcattag cttgtcctgc tgggtagggc taataccgtg caacccaatt cccagcctaa
    14401 ccatgtcgaa ctgggcatgg ggaaaacgct cgataccagc actattcagg atgtggcgaa
    14461 gtggcttata gcctattgca ctgatgattt catccgatat ggtgctgaaa agcgctattt
    14521 ggctttgggt gaactcatcc tgtccggaat cctcgcttgc agccaggtgc gagaagatgc
    14581 ttgcaatcct tatacttttt aacgattgaa tggtggatgt cagctccgga agctgttcgc
    14641 cggtaaaccc caggcgatgc ataccggtat caatcttcag atgaatgttg tattcattca
    14701 cccccatcct gtccagggca ttggcaaatt cggcaagtcc ttcaaagttg tatatttctg
    14761 gttcaagccg gttttcaatc atcagctcgt aggcgccgta ggatggattg agaacaatga
    14821 taggcagagc aataccggcc tcacgaagcg atacaccctc atcggcaaat gccactccca
    14881 ggtagtcaac cctgttgaat tgcaggaggt tggcaatttc gtatgaacca ctgccataag
    14941 agaaagcttt taccagcacc atcagcttta ctcccgggtt gagctggttg cggtagtggt
    15001 tcaggttatg caccatcgag ttcaggttta tctccatcac cgttcgatga actttttcct
    15061 ctaaaatggc tactatccgt tcaaagtgaa agttccggct tcccttgatg agtattgcgg
    15121 agttgctgaa ctgccttcgg gttatgcttt gtgtgaactc aagcgtattg ctgtaaaact
    15181 ccttgcttat atcgaagagg tgagcatagc gtgaaatatt tttaccaatc ccaatgatat
    15241 gggatatttc cttttcttcc accagctggg caaccatgga gtacagcagc tcgggggcat
    15301 agccgctttg caggatatcc gaaaggataa gtacgcggtt gtcatgttgt ttttgctgga
    15361 gtaaaaaatc gagggctatg gaaagcgagc ccaggtcgga gttatagcta tcgttgataa
    15421 tggtgcatcc gtttacccct tttttcaact ccagcctcat ggcaacgggg cttagcttgg
    15481 ccatacggtc agcgatggtg gttctatcaa tattaaggta gaacaacaag cttaggcagt
    15541 gcatggcatt ctcgtaggag gcctggtcgg taaaaggaat ctcaaagctg aacgtttctt
    15601 ccccatactt tacccggatg gctctaccgt tgccatgctc ctccgtactc tctacatgca
    15661 gggttgactg gggatgaaca ccccatgaaa gcagcttaac cccactaagc gtgtcatcgt
    15721 tatagatgat acgatcaacc agctcgtgat ccttacagta aataagcagc tcggatgatt
    15781 tgaaaagttt aagcttctcg gtaatttttt cgtccaaatc ctcgaaattt tcctgatgtg
    15841 gaaggccaat attggtgaaa attccaactt gaggattaat gataggttga agtcgcagca
    15901 tctcgccagg aagggaaatg cccgcttcaa atatcccgag gttgaagttg ctgtccattt
    15961 gcaacaccga aagggggact ccaatttggg agttgtagct tttggggctg cgtacgatgc
    16021 tgtaatcggg cgaaaggagc tggtttagcc actccttaac aatggttttg ccgttacttc
    16081 cagtgattcc gacaactggg tacttaaact gtgcccgatg gtaggtggct agtttttgaa
    16141 gtgcatgcag ggtattctta acgacgataa aggtagcctg gggaaatttt tcggccagcc
    16201 cgagggtgtc gctaaccatg aatgccctta tgtgctgaaa gttgtaaagc tcgctgatga
    16261 atttatgacc gttgtggtgt ttcccaacga tggcaacaaa aagcgttccc ttgcttttgg
    16321 atggggttcg gctgtcggtg gcaattgtgc tgatctccag gttaggatca ccttcgagtt
    16381 tggcgttaag aatttgtgct atttgccctg ttgtgtaagg ttgcatagta tttataggtg
    16441 gatactccgt acaaatttac acgaaatttc caatgttgat tctgctcagc atgtaataca
    16501 ggatgattaa gctacagaat ttactttggc gctaaacggt agagccctaa ggcgattgca
    16561 gcaaagataa tatttggtat ccacaccgca ataaacgggc tgaggtgccc gctcaccgca
    16621 aaaatggtgg agaagcgcat gaataggatg taggagaagc taagggctag gccgaaacct
    16681 atatgcagcc ctatgccgcc gcgaactttc cgtgacgaaa gcgaaacccc aataagggtg
    16741 agaatgaaag ccgaaaaggg attggcaaag cggctatgct tctctattaa aagtgcctca
    16801 atgttatcgg caccctgcag ccgctgctcg tcaatgaagt cgtttagctg aaaaaggttc
    16861 attgtttcaa tcacgttcga acgtctctta aaatcagcgg gagtcaggta taaagtgcta
    16921 tcgatggatt ggccccgagt aatcttttcg cccctttcaa ggtaatcgcg tatgtagtag
    16981 tttttaattg tccaggtttg cttggttgag tcccactggg cataatcggc aatcagcttc
    17041 gattttagcc ggttgttatc aaaatgctca atggaaaggt tgtaggcaat gttgctgtag
    17101 ctgttaaatg aacgcatgta aacaaagatt ccaggtcgaa cctgcctatg gatattgcgc
    17161 tcggagctca cgtaggcttt ttttacatac ttattctcaa agtcaattct tacagcgttg
    17221 gctggaggaa tgatgaagtt ggtgagcacc agggagaaaa ttgcaatgat ggtggcggaa
    17281 ataaagtatg gcaccatcat ccgtaaaaag ctaaccccgc tgctcaaaat ggctattatt
    17341 tcggtgttgt aggccatgtt ggaggtaaag aatatcaccg ctataaaaac gaatagcgag
    17401 ctgaacaggt tggcaaagta ggggataaag tttaggtagt accggaatat gatttcgttg
    17461 aagggtgcac ccttttccat gaagtcgtca attttttccg aaaagtcgaa tataacggct
    17521 atgcctacaa tcaggataat ggcatatatg tatgttccaa taaacttgcg gattatgtac
    17581 cagtcgagta tcttcacggc attaagtttt gagggcgaaa attagtaaat attgttactt
    17641 gattgtatgc cagctaatac ttagcggctc tttggctaaa gaatacttca tttttttaca
    17701 ttttgagagc agcttcacct tcctgaagat tgagttttgt agctttcgaa tggcacaggc
    17761 tacaatcttt gtgaaagtct gctaaccata ctttttttcc actccaaaaa atctccccga
    17821 acaatatgta ttctggcctc cctaaccagc cagaggtaga accccaggtt atggatgctg
    17881 gcaatttgtg ggcccagcat ttccttggcc acaaacaggt gccgcaggta agctttggag
    17941 tagcgtgaat caacaaaagt taggccaaga gggtcaatgg gtgaaaaatc gtttttccat
    18001 ttttcgtttc gcaggtttat tatgccctgt gaggtgaaaa gcattccatt acggccattc
    18061 cgggtgggaa gaacgcagtc gaacatgtca actcccaggg ctatagcctc cagaatattc
    18121 tcgggtgttc ctacgcccat gaggtagcga ggggagttct ttgggagtat gtaattcacc
    18181 acctcaatca tccggtacat ctcctcggca ggttcgccca ccgaaaggcc gccaattgca
    18241 tacccatcgc agttgaacga aacggcatac tcagcggctc gccttcgcaa atcggggtac
    18301 acgcaaccct gtactatcgg gaaaagggat tggctatacc cgtaatgcgg ttcggtggtt
    18361 gcaaactggt ttacacatct tttaagccat tgttcggtta aatcgagcga cttgcaggca
    18421 tagctgtact cggcatcccc gggggggcac tcgtcaaatg ccatcacgat atcggcgcca
    18481 attacccgct gaatgtccat taccctttcg ggggtgaaaa aatgagatga cccgtcaatg
    18541 tgcgagcgga acgaagcgcc ttccgcattt agcttccggt ttgaggccag cgaaaaaacc
    18601 tggtagcccc cgctgtcggt aagtatgggg cggctccatg agctaaaccg gtgaagccca
    18661 ccggcattgg ccaaaacctc cagcccggga cgaaggtaga gatgataggt gttgcccaga
    18721 attatccggg cgtcaatatc gttctccagc tcgcggtgga gcaccccctt aacactccca
    18781 acggttccaa caggcatgaa aatcggagtc tcaatcactc catgatcggt tgctatttca
    18841 cctgcccgtg cagaggtctg gctgtcggtg ccaattagtc gaaagtccat cctgtatatt
    18901 ttcgccgcaa aaatagcaaa attagctatg tggggcggta tggttttgcc ctacgaacaa
    18961 taatttgtat atagaaatgg gtagcttgtt ttgttaccgg tagttttaca ggctgtagcg
    19021 tttgttaaaa atgcccaaaa attttacacg tcaaattgag tgtccatgtg agactgcttg
    19081 caactttcag gtaagcataa atagtagagt ttcttttatt tttacagcat gacaaggcac
    19141 aagcaggtgt cttgtcatgc taatcatccg aactcaccga tatgctatca acgaggcttt
    19201 tgagttgttt ggcctggttc gggctataga tttcgcccaa aaccctagct acttttgcta
    19261 ccgcctcaac aacctgccgg cgctatttca cctgttacct catgtttgta atcgcctaaa
    19321 tgtctggttc aaacaggtag tttagcaaat tactccttta ttgttgggta aacccaaaaa
    19381 tgtttgatat tattgctgga ttttttaaac aatgaatagc ggtgaaagaa attttaagca
    19441 gcatagagac ctatgtgctg gtggtgggca ttttgtttat tgccgccctg gttgttcagc
    19501 tctactacta cctggtagtt tacgcccgag ttgcctgtag taaaagctac aagagattgt
    19561 tgagccccga agctcccaaa ccgcctgttt cggttgtgat ttgtgcccga aatgaggagg
    19621 aaaatttacg ccattttctt ccgcgtattc tcgaacagga ttaccctgaa tttgaggtgg
    19681 ttgtggttaa cgactgctct tgcgacgaat ctgaagtgct gcttgatagg ctcagcaagg
    19741 aatatccccg tttgcaggtg cgaacaatca aggaggatga aaagttcagc cattctaaaa
    19801 aacttgcgct aacagttgga attaaggcag cccggtacga ttggctgctg cttaccgatg
    19861 ccgattgcat cccgcaaagc gataagtgga ttgcaaccat ggcttcaaat tttgacgcaa
    19921 acgcaggtgt ggtgctgggc tatggtggct atcttcatca aaaaggctgg cttaacaagt
    19981 ttattcgctt cgacacactc tacatagcca tgaactacct gggctttgcc ctaatgggta
    20041 aaccctacat gggtgtagga cgcaaccttg cctaccgaaa agatttgttt tttaataata
    20101 agggatttgc ctcatttgtt cagctcgatt ccggcgatga tgatcttttc gttagccagg
    20161 tggcaacccg tcagaacacg cgtgtggatt tccgcaaaga cgcccacacg cgttcggttc
    20221 cacgcagctc acaccacctt tggttgcagc aaaagattag gcatacctca acctcgcgct
    20281 actacaaacc aggagttaaa atcaggttaa cacttgaacc cttgtcccgt ttcctgctgc
    20341 tggcatcagc aatttgcctg gttgcctttc gttttttccc ctactggatt gttgccctag
    20401 tcctttttaa actatctgtt caactaatta tcataaaatc tgttatggta agattgggtg
    20461 aacgaaaaat tttgctggtc tcgcttttgt gggatatata ttcactgtat ttttacccaa
    20521 aacttctact tattaatcgt tttaccagac ggaaaacaac atggagatag ctcccgagaa
    20581 tctatcggaa aaagcgcgct acgactatga gctggttaac agggcactcg agggagatca
    20641 gaagtcctat gccgagctta tggagcgtta tcgcgatgcc atttacttta tgctgctgaa
    20701 aatggtgaac aacaagagcg atgcggagga cctgaccctt gaagctttcg gtaaggcatt
    20761 taagaaaatt caccagtaca ccccaaacta cgccttcagc acctggcttt tcaaaattgc
    20821 taccaataac tgcatcgatt tcattcgtaa acgtaaagcc aacatagttt ccattgacca
    20881 gaatatagat gaccaggaag gccagtacat ttcacctacc tcaatgcttt cttccggaaa
    20941 ccccgacccc gaggaaaagt tgataaagga acagaacatc cagcttattc aggacattgt
    21001 ttccaagcta aagccccgct acagaaaact tgtggagttg aggtacttca aggagtactc
    21061 gtacgaggag attgccgacg agcttaacct tccattggga acggttaagg ctcaactttt
    21121 cagggctcga gagctgcttt ccaacatcct gaataattta ccaccccacc attaaaccaa
    21181 aaatggctgg cagtgcgtgg tagaaagggt tggtaaccgc atgttatcta gtagcggcca
    21241 aacattatgg caataggtaa actcctaaat ttttgcttgg taattatttt acattattga
    21301 taaccattaa gttataagtt ggagaaatgg tgaacagcag tatagtaacc cagtattttt
    21361 caggtctgaa taaggaccag ctggagaaat ttgaaaggct cgatgacctc tatggatact
    21421 ggaatgcaca aatcaacgta atttcacgta aggatattga caacctgatg gttcaccacg
    21481 ttctgcattc cctgtcaatt gccagggtta taaactttgc aaggggaaca aaaataatgg
    21541 atgtgggaac agggggcgga tttccgggta ttcctctggc tatcatgttc cctcaatgcc
    21601 agtttaccct ggtagaatcc gtggctaaaa aggctaaggt atgtggtgaa gttgcccgat
    21661 cgcttggcct cgacaatgtt gaggtgttga acatgcgagc cgaagcacta tcagcaagtt
    21721 tcgattttgt tgtaagccgt gcggttgcca ccattccgca gctgatggaa tgggttgaaa
    21781 agaatatcca ttcgggcggg atgaaccagc tgcctaacgg gctcatctgc ttaaaagggg
    21841 gtagtttttc cgatgagcta aaaccctacc gtaagtttgc gcaaacctgg agggtgagta
    21901 atttttttga tgatgagtac ttcgaggaaa agtacataat ttacattcag gtatagtatt
    21961 gataaacagg aatatgctaa atttaagatt taatagtggt aataattacc ctacactatt
    22021 tttttcagaa aaatttattt ttaactttgc acactcaaat ttgagaaagg aatacgctac
    22081 aagtccatca ggtctataag gcaaaagtaa taatacaaaa agataacgga gatgaagaga
    22141 acattccagc cttcaaacag gaaaagaaga aataagcatg gttttcgcga gcgcatgtca
    22201 acacataacg gccgcagggt actttcagcc cgtagggcta aaggcagaaa aaagttgacg
    22261 gtttccgatg aaatttacca taagtagatt tgttggtaag aatatttcct aatgggaagg
    22321 cgggatgaaa cctgcctttt attttttgtg caactttaag gcaacattcg ttgtttatcg
    22381 aaaagctatc tttgggtgaa aattaatatg gactttaatg caagctatgc tggttgaacg
    22441 ggaaaaaccg ttacggttaa ccgaatcact tggggttata gtggagaagg ttgctgccaa
    22501 ccaacgaatt acggttgagg atgcagttat gctctacgag gatgcagatt tggcgctgct
    22561 ctcgcagctt gccaacatgg tatgcggcag aataaatggc agtaacatat attttaataa
    22621 aaattttcat attgagccga ctaacctttg tgtttacagc tgtaagtttt gttcctacca
    22681 taaagcagcg ggacatccgc agagctgggt gttgagcctt gaggaaatta gggagctagc
    22741 aagccggtat aaaggaagcg atgtaactga ggctcacata gtaggaggag tacacccaaa
    22801 gtggaacatc gacaactatg gtgaaattat ttctgtggta aagcaggttc tccctgaagt
    22861 ccacgtcaag gcattctcgg caattgagct gaactatgtt ttccgcaaaa gcgggctttc
    22921 tccccgcgag ggatttaaaa ggttgaaagc tcacgggctt aactcaatac ccggcggtgg
    22981 cgctgaaatt ttcgactctg gggttcgtgc taaaatttgc cccgacaaag cttcggcgga
    23041 ggagtggctc tccatccacg aagcggcaca tctggagggt atcacatcca acgctactat
    23101 tctatacgga catattgaaa cctacaggca ccgagctgag cacatggagg caatccgaaa
    23161 tctacaggat aaaacccacg gttttagctg ctttataccc ctgaaatttc gttctgccaa
    23221 taaccccctt agctacctgg gcgaagttag cctgctggag gatcttaaga actacgctgt
    23281 tacgcgtatc ttcttcgata acattgacca tattaaagcc tactggccca tgctgggcat
    23341 tgagaatacc cgtttggcca ttgcttttgg agccgatgac attgatggca ctattgacga
    23401 taccaccaaa atatactcca tggctggtgc cgaggagcaa aaacctgcca tgacggttga
    23461 aaaacttacc cagctaatca gagaatccgg caaagttccc gttgaacgca attcgcttta
    23521 ccatgcaata aaatactact aggtgcagtt atctaaattg acaaattttt atacaatgat
    23581 tttttcatgc tcatggttgc tactttttgc taggatctgg ttattagaaa atcggcggta
    23641 gcaaacgctt ttttgttgct gcaatagtgt taaatttgcc acttccacac ctacggttat
    23701 ggagaataag atgacacgtt tttttaagag aatttcggcc aatccctaca tttcaactat
    23761 tctgctgggt attgggcttt ttgttgtatt gctgatagtg ttagggattt cgctaagcat
    23821 atacacccgg catggacaaa cctttgccat tcctgatttc agaggtgaat cggttgacaa
    23881 gttgcccgaa cttgtcagaa aagcggggct aagaatggag gttaccgatt cggtttacat
    23941 cttcaaccgc gcccccggga gcattatcga tcagaaccct aagcccggaa ctcatgtgaa
    24001 gaagaatagg cgggtattcg ttaccattaa tgctaaaaat cccattaaag ttgaagtacc
    24061 cgatgtgaaa gggtacaccc tgcgccaagc taaggccata ctagaacagc agggattcga
    24121 ggttggaaag ctgtccttta cacccgacat gggagtgaac aatgtgctcg accagcttta
    24181 taaaggcaaa ccagccatcc ccggaatgct catccccaaa ggctctaaaa ttgacctgct
    24241 gcttggtatg ggaatgcacg gcgagcgaac aggccttccg ttgcttattg gagttaagtt
    24301 tgaggaggcc aagaatagga ttattgaggc ctcgctaaac gttggtcgca ttaagtttga
    24361 tgaaaccatc gaaacatata gggattcgtt ggaggctagg gtttacagcc aatatcctgc
    24421 atactctgag gcaatttctc ttagttttgg aacacgggtg gatatatggc tcaccctaaa
    24481 cgagtcgcgt atcccagcta ttaagagctt taatgccgac tctattcctg ccgttggcag
    24541 ctataatggg gaggacataa ttgagtagct acttcaaaat cttcatgaaa ggtatcaggc
    24601 attcctcctt tttggcaatt atgactctcc acgtaatgtc ggtggaagct caggatacat
    24661 tggtaaatct ttcttcatat cctataagca atctttcgtc taaaattcaa gcaagctcag
    24721 caatcagcaa atcgaaatct tctgttcaac ttcctttttt cgacgacttc tcaagaagag
    24781 gcgttttccc aactgacgag ctgtgggagg taaccggggt tactgccaac cagctttatg
    24841 ctgttaatcc cccaactgtg ggcgtagcca ccttcgatgc agttgatttc acgggtatgc
    24901 tttactcata tctaaccacc ctgcccacag ctgccgacac catcacctcg ttgcccatta
    24961 acctgaacta cctcccctcc gacagcatct acctttcgtt ttacattcag cccaaggggt
    25021 tgggctatag cccttccaga ggcgactcgt tggttgtaga attctatagc cccatggagg
    25081 gtgcatgggt tagagcatgg tcagcttcgg taaattttgc cgacagcacc ctagctttta
    25141 gaaaccatct tgaaagatca tcaactacga ctcactccaa tgccctcgat cgcaattttt
    25201 ttagggtgat gatgggaata acccaacccg aattcctgtc cgatgggttc aggtttaggt
    25261 ttatgaacta tgcatccatt tcaccaagcc aggaggttca agggttgaag agcaactgcg
    25321 atcactggca cctggacatg gtgtacctgg acaaatatag aaacctgaac gatacagtta
    25381 taaatgacgt cgcatttttt aaacctgtaa gaaatctcct acgaacctac tcatccgttc
    25441 cctggaaaca ctttactcgt gcatttagcg aagagtttcc tagtcctctc gagtttagtg
    25501 ttggctaccg aaatttgggc gatgaggtct ggaatgttac caggcgctat gagattgttg
    25561 acgagtcagg tgcccagccc gactaccttt tcatgggtgg agccgaaaac atccatgagt
    25621 ttcaggatgt cagctataca cgtttttttt catacaactt ccaatcggca tggagcgatt
    25681 cggctaaatt cctcttgaag gcatacttaa taacagagaa taagtctccg ctctctttac
    25741 cctactacct gaactttaac gataccataa gctacaacct taatttttac aactactatg
    25801 ccttggatga cggtactgcc gaatcggggt acggactata tggcgaaggg gctgaaaatg
    25861 gaatggtagc cgttaggttt caaaactacc aggccgatag cctgagcggg gtaatgatct
    25921 acttcaaccg gacctacaac aatgctaacg acatcgggtt caagattaca ctctgggatg
    25981 aacggaacgg gatgccaggc aacatcatct accagaaatc gggcgttaga ccgctattca
    26041 ccgatagcct tgacagattt accgtttacg ctctcgataa ggtggagctg aaagagggaa
    26101 gtttctatat cggatggcag caggagtcga gtggtatgct caacgtcgga ttcgacatga
    26161 acagcaacaa cagcagtaga acactctaca atcttaacgg ggagtgggtt gaaaccaggt
    26221 ataacggttc ggtaatgatt agaccaatat ttggaaaact ctatcaaata cctactaatg
    26281 ttgacattca acaaccttca cttgggtttt ccattttccc gaatcctgca caacatcaca
    26341 tacgcctatc gttacccgat ggctttaccc caaaagctat tcagctgatc aacactgcag
    26401 gtcatgttgt ggcaagccga ccctacgaga ccaacgaact cttttacctc gagggtattg
    26461 ctccaggatt ttacatggtc agggtttcaa atgatatggg taggtgcctg accggtaaag
    26521 ttattgtggt aaaatgattg ataagctcca tccattcttt aacggtgagg ttgacgagga
    26581 gaccgacgag caagccgaac tctacgagca ttttcacttt gatgttgata gggggcaagc
    26641 ccaaatccga atagataggt tcctgaccga taaaattaaa aacgtcagcc gcaaccgcat
    26701 tcaatctgct gccgatgctg gtaatattgt ggtaaatggt aaacctgtta agccatccta
    26761 caaggtaaaa cctcttgact caatttcaat cgtcatggct taccccccca gggatacgga
    26821 catcaagccg gaaaacattc cacttagcat tctgtacgag gacgattcct tactggtggt
    26881 tgataaacct gctggtatgg tagtccatcc cgcacatggc aacttttcag gaacgctggt
    26941 caacgcccta ctataccatt tgcgggatgt tccacttttc agcagcggcg aggtacgtcc
    27001 agggttggtt catcggatag acaagaatac ttcgggaata cttgtggttg ccaaagacga
    27061 ggtgtcaatg aaccgcctgg cgcgacaatt ctttgaacga acaacttccc gaaaatacat
    27121 tgccctggta tggggggata tggaagcgga tgagggaaca attaaaggaa atattggtcg
    27181 tagtgtgcgg gataggaaga aaatgcaggt ttttacagat gaagagcaag gaaaacatgc
    27241 tgttacccac tggcgggtgg tggagcgatt tggatatgtt acccttgtgg agtgccgtct
    27301 cgaaactggt cgcacacacc agataagggt gcactttgaa tatatacatc atcccatatt
    27361 taacgatgag gtgtatggag gaaataagat tcttaggggc acgctccata gccactacaa
    27421 acaatttgtg gaaaactgtt ttgagatatg cccacgccac gcactgcacg caaaaacact
    27481 gggatttaca caccccctat cggggatgga gatgaacttt gaatcgccac ttcctaacga
    27541 tataagtgca cttattgaga agtggaaaaa atactcaaca ggaaaatatt cagagaaata
    27601 agaagattag cacgcttaat acattaaagg tatctctcgt tagccgaatc gcacttaatt
    27661 tttagcttta acgcactgtc cattaaccga attggtttgt tatcgttatc aactgcagca
    27721 aaaatgaatg tggcttctac tgccttgttt ctataatcgg aatacatatc ttcctgataa
    27781 atctcaacca aaacttccat ttttacaagt ccaacttttt gaacggttcc cctaatttct
    27841 acaatcatgt taggttgcac cggggcaaca aaattgactt tacacaccga taccgttact
    27901 acttttttcc gagtaaatcg ggttgcagta atataggcca cttcatccat ccactgcatg
    27961 gccaaaccgc caaacaaagt cccattatta tttaaatctc ccggaaatat aaccttataa
    28021 tgacttgttt tcgaacttgt catctgctag aattttttta caaaattatt caataaattt
    28081 tgattatgaa cattagttca ttatttttgt atcgaacaat tgctcataat atggcacgac
    28141 ttaaaattcc aagaaagata acaaatcccc caatagtgaa gggttttaaa ccatatggtc
    28201 ctgatataga tagtaaaaaa agagagtcaa taatcctcaa ttttgaagag tacgaggcat
    28261 ttcgaatgtg cgattacgat atgtacaacc actatcaggc ttcagttcta atgaatgtat
    28321 cgcgacctac atttacccga atatatgcgt cggcaagaat gaaaatttcg aaagcctttg
    28381 ttgagggtag gcaaattgag tttgaaggtg gaaaagttta cttcgacagc gactggtacc
    28441 aatgcaatag ttgtggatgt catttcaaca atcccgaaat aagcatatca attcagaatt
    28501 gccccttgtg tggcagttca gaagtagaaa ggcataccga agaatcactg ggtgatatgc
    28561 ccagaaattc tgaggatgat ttgtgtattt gtcctgaatg cggttttaca aaggaacatc
    28621 actacggtgt accctgcagg aatgagaagt gtccaacctg caattccccg ctaattagaa
    28681 agtatcatta aacatcgaaa taatgagaat tgctataaca tcacagggaa actcgttgga
    28741 atcgatactc gaccagcgct ttggccactg caaatacttt gtaattgttg atatgcaaac
    28801 tggtgcaatt gaaataatcc cgaaccctta cagcgaagca gaagagcagg ctggaacttt
    28861 ggcggttaag ttgctttcgt ccaaaaatgt gaacaagatt atttcgggcg aatttggtat
    28921 aaaagtaaaa cctttactcg acagcttaaa cattcagatg atcatgtata aaaaaccaaa
    28981 tatacccatt ggaaaagtaa ttgagatgtt gaaccataat aattagtggt atgccaaaaa
    29041 tggatggaac tggtccggaa ggtaaaggtg cagaaactgg acgaaagctg ggagattgta
    29101 atcagctcac ggatgaagaa aaagcatcaa agctggggaa aggactagga aagaaacgcc
    29161 acacaggagg tggacaaggc aaagggaaac gattaaagta taacgaaaaa accaaccagt
    29221 aaaaaggagg taatatgcct ggatttaatc agactggccc tatgggacaa ggtcccatga
    29281 ccggcagacg aatgggacga tgcaccaact atggtgcaaa ccaaggccat acgtcaaact
    29341 ccgaggggaa ctcccaaacg gacgtttttc aaggaagagg cttaggattt ggacgaggac
    29401 aaagaggtcg tggtcgtggc cgaggttttg gaatgcaaaa ccgttccagg ggtggatttt
    29461 agacgattga tttatactat caattaagcc gttcgtaagt aatgtcgaaa agccacccta
    29521 cacctcttac agcagcaaaa cgctgtaggg ggttataagg aaaccgggta aaaagtaacc
    29581 atacgtaagt aaaaaccaaa aatcaaaaat cacatgacga gaaaaagatt tgcagtaccc
    29641 atggaaggta acgtattatc ggcgcatttt ggccattgtc aagcatttgc gtttgttgat
    29701 gttgaaaaca acaatattac caaagtaaca gtactcgacc cacccgagca tcaacccggc
    29761 acattcccta aatgggtggc cgagaatggc gcaaccgagg ttattgccgg cggaatgggc
    29821 cctcaggccg ttaacctatt caacggctat ggtgtaaatg tttttgttgg tgctcctatt
    29881 aaaggggttg aagaacttgt aaacgacttt ttggctggta ggctaagcct aaacgcaaac
    29941 tactgcgacc atggcgatgg cagccacgag catcaccacc acaatggaaa ctgccactaa
    30001 aaccgacatc aatattcaat ctcagtgcta cagttgtttt ttgaaaacat accagcggct
    30061 atttgagaaa tttatgctaa cccaggagca gcaggatgag tttttggatt tcttccacaa
    30121 cactttggaa aaatacaaaa actcaaccag ccctgaggtt cagagagagc taaaccaagg
    30181 tttcagtaaa attgttggaa ttgaggatct ttttgaggat gaaaagatta atagcaacag
    30241 cattgctctg ggattgtata acgattggag cacccaggtc cccatatcaa aaaatccgtt
    30301 tgatttagcc ctaaaacttt ccattgccgg aaatataatg gattacgggg ctaataataa
    30361 ttttgatatc aaatccacaa ttagacgagt aatttttgca gaatttgcta ttaatcacag
    30421 taagcaacta caggataaaa tcaaagaagc aaaatcaatt ctctacctag ccgataacgc
    30481 gggagagata ctctttgaca agctgtttat cgaaacaatc aatcacccaa atattacata
    30541 cgcagttagg ggcggctttg ctttgaacga cgctacgctg cacgacgcta ttgacgtggg
    30601 aatgaactcc gtggcaagag ttattgaaaa tggatttgat gccccagcta cagtgctagc
    30661 aaaatgtagt tctgagttta tggaagttta taattcggcc gacctagtaa tatcgaaggg
    30721 acaaggaaac cttgaaggtt tgatcaacga aaacgatccc cgtatttttt tcttattaat
    30781 ggtgaaatgc gatgttatgg ctagtctgct gaatgttgcc aagggcagtt ttattgtgta
    30841 caacccaacc aaaaacaact aaaatggaaa aagtaaaaat aggcattatc atctgcaacc
    30901 gctaccatac atgtgccgga ggcaaatgtt tcagagcctt acaaaaccgc gagggtgctt
    30961 ttgaaatgta caaggataaa gatgttgaac ttgttggata caccacctgt ggtggttgtc
    31021 cgggcggaaa tattgagtac gcccctgctg aaatgaagaa aaatggtgct actcacatcc
    31081 atttagccac agggctacta gttggttacc caccctgtcc taggctaaac cattttgcca
    31141 aattcatacc cgaaaagtat gggctaaaag tgatatttgg cactcaccct attcctcaga
    31201 aatactttac cacacataca aatctgaaca cctggaactc ctcattctat aaggaggcaa
    31261 taaaagatac acttaccaac gaggctactc gcttgcagta tgattaaaaa agaaaaccta
    31321 aagattgcca ttgccagcgg aaaaggagga acggggaaaa ccttactttc aaccaatctt
    31381 gcagcatttc ttagtgaaag ggaaagtact cttttagttg atttagatgt agaagaaccc
    31441 aacgattctc tttttataaa aggtaaccta atccggaaaa cggaacaaca taaaatgatc
    31501 ccgcagtggg acgagagcaa atgtactctt tgcggggagt gcaccaaaaa ctgtaaattc
    31561 catgctgtag tgcagatggg aaaattcatt gcggttttta aggagctatg ccacagctgc
    31621 tacgcctgct ctgagctttg cccagcaaaa gcattgccca tgcaccctta caaaatgggc
    31681 gaaacggctg agtatagcta caacaatcta aaactaattg aaagtaggtt gatgattggc
    31741 gaggagcaag ctgttccact aatacacaaa acccataagc tggttgaaga aaatttcagc
    31801 aacagcattt ttcaactatt tgattgtcct cccgggacat cgtgccctgt tattgcagca
    31861 accgagaatg ccgattttgt tattcttgtt acagaaccaa caccctttgg gctccacgac
    31921 ctaaaattgg ctgtagaaac catgcgtaag ctgcacaaac caattggagt ggttattaac
    31981 cgctcgggaa ttggtaacga tgatgttgaa aactactgta aaacagagca aattgatatt
    32041 ctcacaaaaa tcccattcga taaaaatatt gcaagctact actcgaaggg agagctggtt
    32101 tacaacaagc ttgagtcggt gaaacattca cttaatgaaa ttctggattc cctaaaaaac
    32161 aaaattccct gtcaatccct ccaaggattt gagaccctta aagggattta atctatcgaa
    32221 acaaatacag aaattcacag atgtaaatga aggaaatagt tgtactatcg ggcaaaggag
    32281 gaacaggtaa aacctcaatt acagcatcgc tggcggtgct tgcaggtagt gaggcggttg
    32341 tggccgattg cgatgtggat gctgccaaca tgcacctact catgcaaccc gattttgctg
    32401 ttgctagtca gttttatagc ggagaactag ccgaaatcaa ccaaaatatt tgcactcaat
    32461 gcggtctgtg ttccaatgta tgccgttttg atgccattcc atttcaaaat aacaaataca
    32521 ctgtaaaccc tttagcgtgc gagggctgtg ggtactgcca aaaggtttgc ccaaccgaag
    32581 caatatccat gaaaaaaaga aaatcggggt acgtttacat ctccaacacc aaggtaaaca
    32641 gcacaatggt gcatgcccgg ttggatattg gcgccgaaaa ctcgggaaaa ttagtcgcta
    32701 aagtaaaaag cgaagctaag gaattggcag agcatcagaa taaatacttc gttattgttg
    32761 atggatcgcc cggaattggc tgtccagtgg tgtcgtcgtt atcaggcgca agctacgtgg
    32821 tgctggttac tgagcctacc gtttccggat tgcacgattt ggagcggata tacagtgtta
    32881 taaagcggtt ccgtataaaa gcaggatgca taatcaacaa atacgacatc aacccaaaaa
    32941 aatctgaaga aattatagag tttctcaata gggaaaacat tgtccacctc gccagcattc
    33001 catacgacat tgcatttacc gaggctatga cacaagccaa aacaatagtg gaaacaagca
    33061 gccgcataag ccaaacgctt atagatgctt ggaacaaaat aaaagaagaa gcagctaaaa
    33121 actaagtacc atgagaattg catttacatc aacagggaaa agctgggatt ctatcattga
    33181 ttcccgcttt gggagaacag agtacatcgt aatcttcgac gaggaaacaa aaacgcttga
    33241 gctagttgac aataccgctg taaagaacga agcccatgga gccggtacag ccactgccca
    33301 aaaaatgtac gaggtaaaac ccgatgtcct tattacaggt aatggccctg gcgaaactgc
    33361 tgcaaccgca ctaaaacgcc ttaatatgaa aatatatgta gatgcccata acctaaccct
    33421 ggaacaagct tacgaaagct ataggaatgg agcattgaag gaaatctaga ataagtccga
    33481 aacaatggag gagtttgtat ttggccccgt tccctcacgt aggctaggga aaagcattgg
    33541 tgtgaacaac ataccccata aggtttgctc ctactcatgt tcctactgcc aggttggcaa
    33601 aggggaaaaa attcagatag agcgtcagga gttttacaat ccggacactc ttgtaaagca
    33661 ggtggaacaa aaactcaaca tgaccagcca tgccgagtac cccgactaca taacagttgt
    33721 gcccgacgga gagcctacgc tggatattaa ccttggcaaa ctaatcacca aattaaaaac
    33781 ttttgggttg cctgttgctg ttattaccaa ctcctccctt atttatttgg atgatgtaaa
    33841 aagagattta tcgaacgccg attacatatc atttaaggtt gatgcagtaa atcacagcat
    33901 ctggaagtat attaataaac cacaccgtaa gctagatcta cacgagatac taaactcctt
    33961 aatggatttt tctaaatact atagggggaa gtatgttaca gaaaccatgc tggtaaaagg
    34021 agataacact gccgatatag agttggcagc caccgcacaa ttcttgcaac aacttaaacc
    34081 gagtattgca tacctttcaa ttcccactcg accaccggct tttgaaaata cattccctcc
    34141 ttgcgaagaa actctactta aagcctacga agttttctac tggcattgcc tgaatgttga
    34201 gtaccttacc ggatacgagg ggaacgcgtt tacctcgtcg ggaaacttcg agaacgacct
    34261 gttgggtatt actgcggttc accccatgcg gattgatgca gttaaagagc tgatggctaa
    34321 aactaaaaca aacgatggtg ttcttgaaaa gctggtagaa gaaaataaag ttaaaaggat
    34381 actctacgaa aggcaaactt ttattgttcg tagcttcaat aagtagattt caaaaaatat
    34441 aaacgccatg ataaaaggaa tacaaaagtc aaagcttggt atcataaccg acattatact
    34501 tttggtattt ctagctctaa tggcaggcat tggtttcctc atgaagtatg ttttgcttcc
    34561 tggcattcag cgaaacagca agtacggagc agatatcaac cttgaattcc ttggtatgga
    34621 taggcatcaa tggggaacta ttcacctaat tacaagcaaa atctttataa ccctgattat
    34681 actgcacatc atcctgcact gggatatgat tatttgtatt cttgataaaa tgataccgcg
    34741 aaaactagta agaagctggt ttttagcaat tttggcaata attaccctaa cacaatttat
    34801 aaacccattc ctggcaagcc cggctctggt tagctacgag aacaactacc ggaacacagc
    34861 cgaaagggtt gaaagcgaac caaatggaat ggatccgctt cacaaaacag caaacgtgga
    34921 agcaaagaaa ccgatacccg accaaagcaa aacttccagg cactctagag ccaaggagga
    34981 gtatggtgta cacggcatcc aaacaataaa cggggtatcg gtacaattta ccataccagc
    35041 aggttttatc ggcaaggaac ttaaaatatc agagggcaac cagtatcaac ctctggttcg
    35101 tcttagaaaa gggtatggat ttactatgtc ggaggtaggc aatgccattg caaaaataca
    35161 agaattctct ggcggcacaa taaagcactt aaaccatgaa ccaaagtaat caacaaaacg
    35221 ggtacatcca cctttatacg ggaaatggaa agggaaaaac aactgcagcc attgggcttg
    35281 ccgttagagc cgctggtgct aacaaaacaa tatttattgg ccaattcgtt aaaggcatgc
    35341 attatgccga gctagtatcg ctccaacgca tcccccaaat taaagtgaag caatttggac
    35401 tcgactgctt tattgtaaac aaccccacgc aaaaagacgt tgatgctgca agagcagggt
    35461 tcgatgaaat ttcgggaata atgctgaaca atagctacaa catggtgatc cttgacgaaa
    35521 tttgcattgc catatactac aggctaatag gtgttaacga ggttatagac gtgcttggca
    35581 aaaaaccacg cgaaatggag gttgttctta ccggaaggta tgcgccccaa gaacttattg
    35641 acattgccga tcttgtaacc gaaatgaagg aaataaaaca ttactacaac caaggaattg
    35701 aagcccgaaa gggtattgaa tactaaccac aaaaaaataa agtatgaaac gaacagatgt
    35761 attagtaatt ggaggtagcg ctgctggaat ggtaaccgca acaaccggta aagcccattg
    35821 gcctgagaaa agtttcacct tagtgaagaa acagaaagat gttatggttc cctgcggaat
    35881 cccttacatc tttggtactc tggagagtag cgagaaaaac atcatgcccg ttgatagcat
    35941 gatggagaaa attggagtta agtcattggt tgacgaagct accgatatcg ataccaaagg
    36001 gaaagtggtg agctttgcca gcggcgaaac aatccagtac gagaaactgg ttattgcaac
    36061 cggttccaat cctgttaagc ctaaatggct tgccggtgcc gaccttgaga atgtttttgt
    36121 gattccaaag gacagggttt acctggatga aatgaaaaaa aagctgggct ccgtaaaaaa
    36181 cattgctgtt attggtgctg gctttattgg cgttgaactt tccgatgagc tggtgaagtg
    36241 cggtcttaac gtaaccctta tcgaaaaact cccgcatata ctaaacctgg cattcgatgt
    36301 ggagctctcc agcaaaattc atgagatgtt aaccgctcgc ggagttaagg ttattaccgg
    36361 agtaggcatc agcaaggttc atggcactgg caaagttcaa tccattgagc tagaggatgg
    36421 acggaaaatg gagatggatg ctgtagttct atcaatgggt tacaggccca ataccgaact
    36481 agctaaaaag gccggaattt ttattgatga ggacaacttc attgctgttg atgagtatat
    36541 gcgcactcac actcctgatg tatttgcagt aggggactgc gctcaaaagc gtgattttgt
    36601 tacccgccgc cgtgtggcaa ccatgctggc atctaccgct tgcgccgaag cacgtattgc
    36661 cgggatgaac ctattcaacc tatccgtagt aaaaactttc agcggtacca tagcaatgta
    36721 ttccactgca ataggcgaaa cagggtttgg aacagctggt gttacagaac aacgcgctca
    36781 acaagaagga ttatctactc taaccggaat tttcgaagga gttaacaggc accccggcaa
    36841 tcttcctgga acgcataagc aaattgttaa gctggttgcc gcatcaaatt ctggagtaat
    36901 tgtaggtggc gaggttattg gtggtcttga agctggcgag cttacaaatg ttattggatt
    36961 ggcaatccaa aaccgcatgt cggttaactc gttcctaacc atgcaaattg gaactcaccc
    37021 atgtttaacc gcatcgccag ccgcctatcc acttattaaa gcagcagaac aaatagctgg
    37081 taaaatgatt aaaaaatagc ccttatgaat cctatcgaaa aaattgagct agatggcgta
    37141 aagaacatcg tggttgttgc ctcggggaaa gggggtgttg gaaaatcaac cgtatcgtcg
    37201 aacctggcca taagccttgc ccgaaacggt tacaaaacag cccttgttga tgccgatatt
    37261 tttgggccat caattccaag aatgtttggc attgaaaaag cgcaaccgga ggtaacgcat
    37321 ctgggcgata aggaggtgat gttcccaatt gaaaagtatg gtgtaaaaat tatgtccatc
    37381 ggatttttta ttaagaaaaa ccaaggctta atctggcgtg gccctatggc tgccaatgca
    37441 attatgcaac ttttcgagaa taccctttgg aatgatatcg attatatgat tatcgacttt
    37501 cctccaggaa caagcgatat acaactcacc acggttcaaa aactcaatgt tacaggggct
    37561 attattgtca ctacacctca ggaggtagca ttaaacgatg ctcgcaaagc agcatcgatg
    37621 ttcaataatc ctgatttgaa tgttccaata tggggtgtaa ttgagaatat ggcctggttt
    37681 actcctcaac cccaccccga tgagaaatac tatatatttg gtcagggtgg tggcgaaatg
    37741 ctggcaaaag aactcaacac aaagttgcta gggcaaatac ccttagtagc ggaggttggt
    37801 gacgctgctg aaaaggggct tagcgtttac aaccaaagca acccaacagt aattgaggcc
    37861 tttgaaaagc taaccgagac tattacagcg tagagtaatc atccaaacag acccgatatt
    37921 tattctttcc gggtctgttt ttttggataa atacactcct acaaccaatt atcaatggaa
    37981 tcgctaaaaa acagaatact aaattgcacc aaatgcgatt tgtacaaaac aaggaataat
    38041 ccaatttttg gcgagggaaa tatacacgct ccaattttta taattggcga agctcccggt
    38101 cgcgaggaag atttagttgg tcgaccattt attgggaaat caggtcaatt actcgataag
    38161 atactggctg catgcaactt caaccggaac tatcatgttt ttatcagcaa catagttagg
    38221 tgtaggccac ccgaaaaccg tgtaccaact tcccgcgagg ccgaaatttg catgccatac
    38281 ctgttggaac agattgaact ggttaacccc aaaatattag tactgctagg ctctacggca
    38341 cttaaataca tggctggtgc agatcataga atcactaaag aaagaggtac ttggctggaa
    38401 gtttatggcc gtttggccat gccagtttac catccggccg cattactccg cgacccctcg
    38461 ctgaaacgcg atacctggga ggattttaag aagatttttt ataagtaccg cgagctggta
    38521 aaccccgaac atcaatcacc acacattagt attggctaaa attagccaat aattggaata
    38581 ttccctttaa ccttatcgtt tgtgcgattg ttggcttgaa agtcaaaggc aggtaaaggt
    38641 cgaccctaga tacaaacacg ataaattcca actcatcgcc ttgctccgca gtatcgcctt
    38701 gtttaacatc gaatataata cgactagcca caaaacctgc tatctggcaa acaatacccg
    38761 aacgctattt ttaacgatga ggtgtatgga ggaaataaga ttcttagggg cacgctccat
    38821 agccactaca aacaatttgt ggaaaactgt tttgagatat gcccacgcca cgcactgcac
    38881 gcaaaaacac tgggatttac acacccccta tcggggatgg agatgaactt tgaatcgcca
    38941 cttcctaacg atatggccca gctggttgat cgttggagaa gctacacggc gggtcgcagg
    39001 atggcataag ctgggttacg ccatagtttt cctagttagg atggcatgat gatgtgcatt
    39061 ctgaatcggt taccccatat tctacctgaa tggtggaatg ctcaatgttg aactgctttt
    39121 gcagctgttg ctggattgtt gatatgaagt ttaaatcagt ttgcttgctg gtgcaaaggt
    39181 gaactgaaag tgctacatct gtggtgctta gcgcccagat atgcaggtcg tgtatgccct
    39241 tcacctctgg aagctgcttc aagtagttgc taacagcctg tatgttaata ccttcaggta
    39301 ctgcgtcgag tgctagcgaa accgaatcaa ccagcagcct tatggcgcta agaagaatta
    39361 tggcgacaat aaccagggag acaagcggat caatccaaac aaaacctgta aaagttatca
    39421 taacccctgc agcaacaact ccaagcgata acaaggcatc ggcaacaaag tgaacaaagg
    39481 tgcttcgaat atttaaatct ttctttttgt cctttataaa aagccaggct gttaggccat
    39541 tgaccagtat cccaacagca gcaattacta taacactcct cgtgtttatt tccgaggggt
    39601 ttcgcagcct gtcaatcgcc tcaattgtaa taaacactac tacggccaaa agtaaaattg
    39661 tgtttagcat ggccaccaaa atcgttgagc gtcgaaaacc gtaggtaaac cgttttgtag
    39721 gttttcggtg tgaaataaca actgctatcc aggaaaatgc cagggcaagc gtgtcgctta
    39781 ggttatgccc ggcatcagct ataagcgccg atgaattgga aagaaaacca aatgcagatt
    39841 ctacggaaat aaaaaccaca tttatggcaa tgccaatttt aaaggcgctg ctgagaccct
    39901 tgctgctatg gctggtatgg ctaaactcca ttttaaaatg ataaaacctt atcacactca
    39961 acaatccacc gggtaaactc tttcatgttg cttggttgca ctccttcaat aagatcaaca
    40021 gcctccatac cccttgccga caggcagcta ccgcaactct taacttctcc tccttgccta
    40081 atcacggcat caagcatacg ttcaatattg tagtagccat tgggagtggt ctggtttggc
    40141 agtccgcaaa aaacaccatc ggccattaaa aaaaccttca cgtttagctc atccccatgc
    40201 tccttttgca gggtcatggc agttctcatg gcattatacg ccttttctgt tccgtatggc
    40261 gactcgttaa ttacaatcag gatgttctgc atcgttttat ttttgttggt aacacattat
    40321 actattgttt gttcgttata acactatgtg cttttatgct gcataaaagt agtgatatgt
    40381 tgaaaatgtg taatctttgg ctatgaacat ttttaagggt atttttttga aaattaagta
    40441 ccctttacaa tcgctgctgg ttggttaatc ccggctgatt atttttattg ggcaaattgc
    40501 aataattttg cttttgtggt ttacgttata tgcttcgttt atgattatga agatggggat
    40561 tgcgcaaaat gattggtgta gggttgcttt agcgactgct tttcttgctt ttgccttttt
    40621 ttcgtgcaaa gacaaacctg aacatttcaa taggagcatc tatgagcaaa agtctggagt
    40681 agctattgtt gccgacacta ttacctatga tgttcagata ggagttaccg atacctccga
    40741 cctatggtca atggaatgcc tgaagtacat gaatcagcag gtttttattg attatatttt
    40801 taatggcatc tacaagggca gcatggttgc tcatgatttt aataatggta tgattattaa
    40861 cgctgaaaag gtaagacagc tggagcaaca acccggattt tcaaggggga aaatcaccaa
    40921 aattcagttt aaggagcttt ggtacatcga ctctttgggc attttcagaa aagaaattat
    40981 tagctacacg ctggggctag aagcgtacag caaccagaat acgtttttgg gctatagccc
    41041 tgtatttgta gttaaaccta caaacgtgca tccataataa acctggcatt acttcgcgta
    41101 cgctgctgga ggtaaactat cctttcgcca accatttcat ctactacatg gctggtatag
    41161 tgcctaatgg tgagcagttc aaggttgctg ttgaatcgaa ccgtgaaggt tttttgaagt
    41221 tcttctatgg ctcctggtag gcgtaaatgt tcatcgtcaa cacaaatggt gaagctgata
    41281 gccgaatttt gaactaagtt caccttaacc cggtgccggt aaagaatggc gaatattttg
    41341 ctgaggcttt cctcaatgac aaaggaaaaa tcgtgtggag tgagcgatac caatgcttga
    41401 ttgcgcttta gaattagcac ggggggtaag ctcaatggga catcagagtg gccaatggtt
    41461 gtacccgaag cttcggggtt gataaacgat tttacataca agggaatttg cttattctga
    41521 atgggtttta tggttttggg atgaattacc tgggctccgc agtaggccag ctcaatggcc
    41581 tcctggtagg atataaaatc aagtttacag gtgttggaaa agatttttgg atcagcgctc
    41641 agcactccgt caacatcctt ccatatagtt acatcggttg catcgagcag gttggctagc
    41701 acggcagccg tgtaatccga tccctctcgt cctagcgttg tagttgtgcc ttcggcagtt
    41761 ccggcaataa aaccctgggt gataaataaa tggtcggagt caaagcaaaa ctctttttcg
    41821 cagagatact gtgatagctc gaaatcaacc accccctccc ggtacgatgt atcagaacgt
    41881 aagcacttcc gaatgtcaat ccacctattt cttatgccag cttggtttag gtaaaagcta
    41941 accatggtgg ttgacagaag ctctccatac gatacaacct ggtcatagca gtagtcgtaa
    42001 cccccacttg gaggaaattt catcttctca tcaagctcgg tgaatagctg ttcaaccttg
    42061 gctaatagag gatgtgaaga gctgctgtcg cctaccagct ccgtggctat gtgggtatga
    42121 aaaactttta gctgatccaa cttattagct ttttcgtcgc tttttgctaa atacagggaa
    42181 agaatctcct ctaatgcgtt ggttgttttc cctatggccg aaaccaccac tacgagattt
    42241 cccgatgttt gccgacggat aatttcggcc atattcctta tcccttctgc cgatttcacc
    42301 gaggcgcccc caaatttgaa aacacgcaat gccgacatat tctttaaaaa tttattcaaa
    42361 catacatgtt tgattcgatt tgggcaactt atcgatataa tcccggcttt caagtgtaac
    42421 gttcttgtat atcttccgca aacgttttaa ctttggcaat tgtgttactt aacatgtttc
    42481 gatttgggta aattggttat ctttatcaac gttaatcagt agcatttttc tgccccatga
    42541 atggatataa tgtaaaaacg ctcacgcagt tcatcattga gcggcaagcg gatttcccct
    42601 atgcaactgg cgaattcacc aggctgcttt accaggtagg tgtagctgca aagcttgtaa
    42661 acaaaaaggt taataaggct gggctcgtgg atgtgctggg cgaggctggc aacatcaacg
    42721 tgcagggaga ggagcagaag aaactcgacc tgtttgccaa ctggcagttc atacaagctc
    42781 tcagaaacag ccgagagtgt tgtggaattg tttcggagga ggaccaggag attataacat
    42841 gggacgatga gttctcacgc gatggcaact acctgctctg tatggatcct cttgatggct
    42901 catccaatat tgatgttaac gtttccattg gaacaatttt cagcatttat cgacgtatca
    42961 gcccccgggg agagaaacct accctagaag atttccttca acctggcaac aagcaggctg
    43021 cagctggtta cgtgatttac ggttcttcaa ccatgctggt ttatacaacc ggccgcggag
    43081 tttacggttt tactcttgac ccctccattg gcgaattttg cctttcacac tttagtatta
    43141 ctacaccttc cgatggaaaa atatactcca tcaacgaggg aaactacgat cattttcctg
    43201 aaggagtgaa agtttacatt gattactgca agcaacagga taagcctacc cagcgaccat
    43261 actcagcccg ctacattggc tcgctcgtgg ccgatttcca tcggaacctg ttaaagggag
    43321 gaatatttct ttatccgccc accgccgcag cacctaatgg taagctgcgg ttaacctatg
    43381 agtgtaaccc tattgcattc attgccgagc aagcgggggg aacggcaacc gatggtagcc
    43441 agcgaatcct cgatataaag cccgaatcgc ttcaccaacg ggttcctttt tacgtgggct
    43501 cgtcaaatat ggtgggcaaa cttcaggagt ttattgataa acacgagtaa caattacctc
    43561 cagctaaaat taaactgctt acgccaaata ttttaccgga ataaggcttg agcgtgttga
    43621 ccctactggt gatgggttga acttgtagct ctatattacg ttggaggttt tcaatgctgt
    43681 tggcctttca tcaaccctga taagccagtt ggttttggtt gaagcttaag gcttcgcctt
    43741 taatggtttc gtcaaatttt cctttatgat agacaatatg accgtttata aaggttgtct
    43801 caatgctgtt atggaaaatt tcaccctcta acggtgacca accacacctg tagtgaagtg
    43861 attgggtagt tactgtttcg gtagatgttg gattaatcag caccaaatcg gcatggtagc
    43921 cctttcggat atagcctctg cggtcaatcc tgaaaatggt ggctggagca tggcacatct
    43981 tctcaaccac cttttctact gaaaagtacc cctttttcga aagttccagc ataacttgta
    44041 gtgaatgctg aaccattggc attcctgagg ctgacttaaa gtaggtacta tccttttcga
    44101 aggctaaatg cggagcatga tctgttgcca cggcatcaac caaaccacta cttatgcctt
    44161 ccagaagagc ctgcctgtcg gattccagct taatacttgg attgcatttt atcctccatc
    44221 ccaacctgtc atagtccctt tcgctaaacc ataggtgagg gatacatgct tctgaggtaa
    44281 tttttttatc agccagagga atactattat tcataagcga aagctccctg gctgttgaaa
    44341 gatgaagaat atgaagtctt gaaccatgct tcttggccaa ctctactgct agcattgaag
    44401 aaatcataca ggcttcggca ctgcgaatac tggagtgaaa tctaaaagga atgttttctc
    44461 ctttttcttg cttgattttt tccgcatttt gacgtatgct ggcctcatct tcgcaatgag
    44521 caacaatgat tgtaggcgac agcttaaaaa tcttttcgag tgcttcccgg ttgctcacca
    44581 acatgtctcc ggttgacgag cccataaata gcttgatgcc acatactttc aatggatcaa
    44641 tttttaccag ctgctcaatg ttatcatttg ttgcccccat gtaaaatgaa tagttaacca
    44701 gcgatttttc agcagctgta ctaattttat catcaagaag ctcaagagtg gtggtttggg
    44761 gaatagtgtt gggcatgtcc attatagagg taaccccacc tgctgctgct gctttcgatt
    44821 cgctgtatat atccccttta ctggttaatc ccggctccct aaaatgaaca tgctcgtcta
    44881 taacccctgg aattagtagc aatcccctgg catctattgt atgttctgga ttataacccg
    44941 ttagttgagt tgaggctcca gtaattattt cttctattat tcctctattt attattactg
    45001 tcccttgaaa cctattcccc tcgttgataa tcgtggcacc agctactgca attcgtccca
    45061 tcataattct tttaacgctt ttatgcaaca ccactaatat tccaaaaact ctaatgctag
    45121 tagtatttta aatatagaag ttgctatcgc tcaggtagat tcaacttttg gtagggcttg
    45181 aatatacttt ttagcttcat ggcaattact ccccaaagag cctcgttgaa aatgcctccg
    45241 ctcatttttg aaactccaaa ttttctatca gtaaatatta taggtacctc cacaatttta
    45301 taccccagct tccatgcggt aaacttcatt tcaatctgaa agccataccc aacaagctta
    45361 attttatcaa ggtttatagc ctttaaaacg cttcccttat agcaaacaaa gcctgctgta
    45421 gcatccataa ttctcattcc ggttatcaac cttacatatt tcgatgccat atacgacatg
    45481 agtactctgc caattggcca attaaccaca tttaccccat ttatgtatct tgaaccaatt
    45541 gctatgtcgg caccatccct gcaggcctta taaagattga ccaaatcgtc gggattatgt
    45601 gaaaaatccg catccatttc aaatatatac tcgtaaccat tggcaagcgc ccacttgaaa
    45661 ccacaaatgt aagcagtccc taatcctaat ttccccggcc tctctatcaa tgaaacccta
    45721 cctgggaaat tatgctgaat ggcttttacc ctttctgctg tcccatcggg tgaagcatca
    45781 tcaataatta aaacattaaa atccccctca agctccatca ccctggctag catcttatct
    45841 atattctcaa gttcattata ggtcggaata attactaact tttctttact gggcattgtt
    45901 atgtattatt aagttagggt tggcatatgt aatgtgaata tgcgaatata atgcttttat
    45961 gcatttggtt tgagagaatc catctggttt tcaaaatcag acaaaattaa attatatgag
    46021 aaccaaataa ttaaacatcc aaaataaatt ttattttaaa aagatgtaaa aaaggtgtaa
    46081 aaaagtttgg atggaattgg gggaggtggt atatttgcag cc
//
LOCUS       DAOSHM010000016        43561 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_678_length_43561_cov_20.684825, whole genome shotgun sequence.
ACCESSION   DAOSHM010000016 DAOSHM010000000
VERSION     DAOSHM010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 43561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 43561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43561
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_678_length_43561_cov_20.684825"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            245..1084
                     /gene="lgt"
                     /locus_tag="PK839_04130"
     CDS             245..1084
                     /gene="lgt"
                     /locus_tag="PK839_04130"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOG71958.1"
                     /translation="MLVFIHWNVNPEIFSIGNLSVRYYGLMWALAFYLGYVIFNRFVK
                     KEGLPNGFLDSLTMYMLIGTVLGARLGHCLFYEPSYYLSHPLEILKIWKGGLASHGAA
                     LGILIALYLFGRKHKVPMIYVVDRVVITVALGGALIRIGNLMNSEIYGVKTNLPWGFI
                     FERNNEIYPKHPTQLYEAISYLAIFFLLHYIYRKNEGKLKTGFIFGLFLVLLFMARFL
                     IEFLKEPQVDFEAKMTLNMGQLLSLPFIAVGIAFIIYSQVKPSYYTPPKQTPVQKSKK
                     SKG"
     gene            complement(1322..3478)
                     /locus_tag="PK839_04135"
     CDS             complement(1322..3478)
                     /locus_tag="PK839_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOG71959.1"
                     /translation="MKTYSTDQIRNIALIGNTGSGKTSLAESMMFEGKVIERKGSIEG
                     KNTISDYTEIEQLNQRSIFSTVLYTEYMDHKLNVIDTPGSDDFVGSVITSMYPTDMVV
                     VTINAQYGVEVGTEIFNRHAERLERPILIAVNQLDHEKANFEQAMDSLRQTYGTKVLQ
                     TQYPLNTGSLFDSFIDVITMKMYKFKDDTGIREEFPIPAEEMEKATALHNTLIESAAE
                     NDETLMEKFFEQGSLSEEEMRKGLSIGVRNRQIFPVFCLSAKKNIGTKRLMEFIIKIG
                     PSPASTPMLKNNSKTEIKCDAAAPTSLFIFKSTVEPHLGEINYFRVMSGKIAEGMDLT
                     NANNSTKERLTQIFAVAGKNRTKVSEMVAGDIGATVKLKNSKTNQTLCGPGIDFTFEP
                     MQFPNPKFRTAIKPKNEAEVEKLGELLNRAHQEDPTIEVEHSKELKQIIISGQGEHHL
                     NILKWQLTNLNKLEVEFIPPRIPYRETITKIAQADYRHKKQSGGAGQFGEVHLVIEPH
                     VEGAPDPVKYKINGKEINLSVRGKEEIKLDWGGKLIFYNCIVGGSIDARFMPAILKGI
                     MEKMEQGPLTGSYARDIRVAVYDGKMHPVDSNEISFKLAGRNAFKNAFKEAGPKIMEP
                     IYDVEVLVPSDRMGDVMSDLQNRRAIIMGMGSTKGFEVINAKVPLAELNKYSTILSSL
                     TSGRATYTMKFASYEQVPTDVQEKLLKAYEAEEEEE"
     gene            3984..4361
                     /gene="rpsL"
                     /locus_tag="PK839_04140"
     CDS             3984..4361
                     /gene="rpsL"
                     /locus_tag="PK839_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HOG71960.1"
                     /translation="MPTIQQLVRKGRSKMVDKSKAPALDACPQRRGVCVRVYTTTPKK
                     PNSAMRKVARVRLTNGKEVNAYIPGEGHNLQEHSIVLIRGGRVKDLPGVRYHLIRGTL
                     DASGVEGRKQRRSKYGAKRPKKK"
     gene            4394..4870
                     /gene="rpsG"
                     /locus_tag="PK839_04145"
     CDS             4394..4870
                     /gene="rpsG"
                     /locus_tag="PK839_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009597682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HOG71961.1"
                     /translation="MRKSKPKKRLILPDPKFGDVMVTRFVNNLMERGKKSVAFDIFYQ
                     SLDIVGQRMKDADKNPLEIWKKALENITPQVEVKSRRVGGATFQVPMEVRPERKISLS
                     MKNLILYARKRSGKGMSEKLAAEIMAAYNEEGAAFKRKEDMHKMAEANKAFAHFRF"
     gene            4904..7021
                     /gene="fusA"
                     /locus_tag="PK839_04150"
     CDS             4904..7021
                     /gene="fusA"
                     /locus_tag="PK839_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOG71962.1"
                     /translation="MSRDLKYTRNIGIMAHIDAGKTTTTERILFFTGKTHKIGEVHDG
                     AATMDWMVQEQERGITITSAATTTFWRYNDQQYQVNIIDTPGHVDFTVEVERSLRVLD
                     GTIATFCAVGGVEPQSETVWRQADKYRVPKIAFVNKMDRVGADFLSVVGQIKEKLGAN
                     PVPIQLPIGAEDTFVGIVDLIENKAYVWNDDQKSNSNNFRVEEVPDSMKQEVAEWRSK
                     LVEAVASYNDDLLERFFDNPESISNDEIIDTIRRATIDMSIVPVLCGAAFKNKGIQKL
                     LDAVIAFLPSPLDKGNIKGVNPETGNEEIRKPYINEPFCGLAFKIATDPYVGRLAFIR
                     VYSGKIDSGSYVYNVRTGNKERISRLYQMHANKQNPIEFCEAGDICAAVGFKDIRTGD
                     TLCVESNPIALESMTFPEPVIGLAIEPKTQKDMDKLGIALNKLSEEDPTFRVTSDPDS
                     GQTIISGMGELHLEIIVDRLRREFNVDINQGAPQVAYRETLTRTVSHREVFKKQTGGR
                     GKFADIIFEIGPADEGVQGLQFINEVKGGNIPKEYVPSIQSGFDQAMNNGVLAGFALT
                     NLKVVLKDGSFHPVDSDALSFEIAARYGFRAAAPKASPVLLEPIMAVEVVTPEEYMGD
                     VIGDLNKRRGQITGMESKGVARVVKAKVPLSEQFGYVTVLRTLTSGRATSTMEFSNYA
                     EVPASIASEIIEKSRGKVVKEIE"
     gene            7043..7348
                     /gene="rpsJ"
                     /locus_tag="PK839_04155"
     CDS             7043..7348
                     /gene="rpsJ"
                     /locus_tag="PK839_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005782187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HOG71963.1"
                     /translation="MSQKIRIKLKSYDHNLVDKSAEKIVKTVKATGAVVSGPIPLPTH
                     KRIFTVNRSTFVNKKSREQFELSSYKRLLDIYSSTQKTIDALMKLELPSGVEVEIKV"
     gene            7375..7995
                     /gene="rplC"
                     /locus_tag="PK839_04160"
     CDS             7375..7995
                     /gene="rplC"
                     /locus_tag="PK839_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HOG71964.1"
                     /translation="MPQGLIGRKIGMTSVFVEDGKNVPCTVIEAGPCVVTQVKTVESD
                     GYTAVQLAFDEKKEKQTTAPMMGHFKKANTTPKRKLAEFRDWSGEMQLGDVITVDIFN
                     TDRWVDVTGITKGRGFQGVVKRHGFGGVGGQTHGQHNRQRHPGSMGASSYPSRVFPGK
                     RLAGRMGGDRVKIINLRVLKVIPESNLLLVKGSIPGAKGSYLIIEK"
     gene            7995..8627
                     /gene="rplD"
                     /locus_tag="PK839_04165"
     CDS             7995..8627
                     /gene="rplD"
                     /locus_tag="PK839_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009132862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HOG71965.1"
                     /translation="MEAKVLSISGQDTGRTVQLDDSIFAIEPNDHAIYLDVKQILANK
                     RQGTHKSKERSEVSGSTRKLKRQKGTGTARAGSIKSPLFRGGGRVFGPVPRDYSFKLN
                     KKVKLLARKSALTYKVKENAFIVVEDFNIETPKTKEFITICKNLNVADKKTIFVLSET
                     NKNIYLSSRNLQQQKVVQASSLNTYEILHAKAMVLCESSVEVLHKMFKIS"
     gene            8640..8930
                     /gene="rplW"
                     /locus_tag="PK839_04170"
     CDS             8640..8930
                     /gene="rplW"
                     /locus_tag="PK839_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HOG71966.1"
                     /translation="MDILIRPIVTEKMERLTGKLNQYGFIVDKRANKFQIKDAIQDMY
                     GVTVDRVNTMRYAGKRKSRYTRTGVVTGRTNSYKKAVVTLKAGDKIDFYSNI"
     gene            8936..9760
                     /gene="rplB"
                     /locus_tag="PK839_04175"
     CDS             8936..9760
                     /gene="rplB"
                     /locus_tag="PK839_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015547049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HOG71967.1"
                     /translation="MAVRKLKPVTPGQRHKIIGTFEEITTSKPEKSLLRPLKKSGGRN
                     NQGKMTMRYIGGGHKRRYRLIDFQRSREDEPAVVKSVEYDPNRTARIALVEYPDGEKR
                     YIVAPNGIKVGQTVMSGHGVAPEVGNTLYLSEIPLGTLIHNIELYPGRGACMARSAGS
                     YAQLLAREGKYVVVKLPSGETRMVLATCKATIGTVSNPDHALEKSGKAGRSRWLGRRP
                     RNRGVAMNPVDHPMGGGEGKASGGHPRSRKGIPAKGYKTRSKKKASNKFIVERRKK"
     gene            9782..10051
                     /gene="rpsS"
                     /locus_tag="PK839_04180"
     CDS             9782..10051
                     /gene="rpsS"
                     /locus_tag="PK839_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007212071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HOG71968.1"
                     /translation="MSRSLKKGPFIDYKLEKRVIEMNSTSKKSVIKTWSRSSMISPDF
                     VGHTIAVHNGNKFIPVYITENMVGHKLGEFAPTRTFRGHAGNRKK"
     gene            10079..10495
                     /gene="rplV"
                     /locus_tag="PK839_04185"
     CDS             10079..10495
                     /gene="rplV"
                     /locus_tag="PK839_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HOG71969.1"
                     /translation="MGARKREMANRLKEERKDKAMAVLRNCPSSPRKMRLVVDQIRGK
                     DVNTALNILKFSPKHASKDVHKLLLSAIANWKSKNEGIRLEDANLFVKEIFVDQGRTL
                     KRIRTAPQGRAHRIRKRSNHVTVIVDSAPAINDTNN"
     gene            10503..11234
                     /gene="rpsC"
                     /locus_tag="PK839_04190"
     CDS             10503..11234
                     /gene="rpsC"
                     /locus_tag="PK839_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HOG71970.1"
                     /translation="MGQKVNPISNRLGIIKGWDSNWYGGKNYSQKLVEDYKIREYLNE
                     RLSKASLSKIVIERTLKLITVTIHTARPGIIIGKGGQEVDKLKEELRKITNKEVQINI
                     FEVKRPDLDAVIVSNNIARQLEGRISFRRAVKTAIASTMRMGAEGIKVQVSGRLGGAE
                     MARSETYKEGRIPLHTFRADIDYALAEAHTKVGLIGIKVWICNGLVYGKRDLSPNLGI
                     SSAAPAQSGHGQARPHGARKKKSNK"
     gene            11257..11685
                     /gene="rplP"
                     /locus_tag="PK839_04195"
     CDS             11257..11685
                     /gene="rplP"
                     /locus_tag="PK839_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019152134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HOG71971.1"
                     /translation="MLQPKKTKYRRQQKGRMKGNAQRGNQLAFGSFGIKALDSAWITS
                     RQIEAARQALTRHMKREGQIWIRIFPDKPITKKPAEVRMGKGKGAPEGFVAVVTPGRM
                     LFECEGVSLDIAKEALRLAAQKLPIATKFVVRNDYVEPVS"
     gene            11693..11893
                     /gene="rpmC"
                     /locus_tag="PK839_04200"
     CDS             11693..11893
                     /gene="rpmC"
                     /locus_tag="PK839_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HOG71972.1"
                     /translation="MKASEVRELTDKEIVERIEAEKTNLLKLKLNHSISPLDNPMKIK
                     ESRRTIARLKTILTQRKLSQNQ"
     gene            11906..12169
                     /gene="rpsQ"
                     /locus_tag="PK839_04205"
     CDS             11906..12169
                     /gene="rpsQ"
                     /locus_tag="PK839_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HOG71973.1"
                     /translation="MEEVKRNLRKVRTGVVVSNKMDKSIVVAVHRKVKHPTYGKYVKK
                     TTKFYAHDEANTCNIGDVVKIMETRPLSKLKRWRLVEIIERAK"
     gene            12172..12537
                     /gene="rplN"
                     /locus_tag="PK839_04210"
     CDS             12172..12537
                     /gene="rplN"
                     /locus_tag="PK839_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HOG71974.1"
                     /translation="MIQQETRLKVADNSGAKEVLCIRVLGGTGMRYARIGDVIVVTVK
                     NAIPSGEIKKGTVSKAVVVRTKKEIRRPDGSYIRFDDNACVLLNAQGEIRGTRIFGPV
                     ARELRDNYMKIVSLAPEVL"
     gene            12560..12883
                     /gene="rplX"
                     /locus_tag="PK839_04215"
     CDS             12560..12883
                     /gene="rplX"
                     /locus_tag="PK839_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010262408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HOG71975.1"
                     /translation="MASKLHIKKGDLVYVISGESKGKQGRVLSVQVSKQRAFVEGVNM
                     VSKHTKPNSKYPQGGIIKMEAPIHISNLMLIDPASGKPTRIGRRLNDKGKLVRYSKKS
                     GEEIK"
     gene            12883..13440
                     /gene="rplE"
                     /locus_tag="PK839_04220"
     CDS             12883..13440
                     /gene="rplE"
                     /locus_tag="PK839_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HOG71976.1"
                     /translation="MEYVANFKRKYKEEIVPALMKEFGYKSVMQVPRLEKIVINQGVG
                     QAVSDRKIIEAAQADLTLIAGQKAVQTFSRKDISNFKLRKNVPIGLKVTLRRDRMYEF
                     MERLINVSLPRIRDFKGINDKLDGRGNYTLGIEEQIIFPEIDMDKINRLLGMEITFVT
                     STNNDEEAYALLREFGLPFKNAKKN"
     gene            13444..13713
                     /gene="rpsN"
                     /locus_tag="PK839_04225"
     CDS             13444..13713
                     /gene="rpsN"
                     /locus_tag="PK839_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HOG71977.1"
                     /translation="MAKESMKAREVKRAKLVEKYAEKRAKLKAEGDFIGLQKLPRNSN
                     PIRLHNRCMITGRPRGYMRQFGVSRITFREMASKGLIPGIKKASW"
     gene            13741..14136
                     /gene="rpsH"
                     /locus_tag="PK839_04230"
     CDS             13741..14136
                     /gene="rpsH"
                     /locus_tag="PK839_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004335784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HOG71978.1"
                     /translation="MTDPIADYLTRIRNAVMANHRVVEIPASKMKLEMSRILFEKGYI
                     LNYKFEDDGKQGTIKIALKYHPETKKNAIKHIERVSRPGLRKYTNARELPRVLNGLGV
                     AIISTSQGIMTDKEARVKNIGGEVVCYVY"
     gene            14168..14722
                     /gene="rplF"
                     /locus_tag="PK839_04235"
     CDS             14168..14722
                     /gene="rplF"
                     /locus_tag="PK839_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HOG71979.1"
                     /translation="MSRIGNKPVNLPQGVSVKISADNVVSVKGPLGELSQKVDPDITV
                     NLQGNLVVLTRPTDQKRHRSLHGLYRALINNMVIGVSTGWELQQELIGVGYKAEAKGQ
                     LLQLSLGFSHDIDFLLPNEVKVETKTERKGNPTITLKSIDKQLLGQVAAKIRSLRKPE
                     PYKGKGIRFVGEVVRRKAGKSASV"
     gene            14744..15103
                     /gene="rplR"
                     /locus_tag="PK839_04240"
     CDS             14744..15103
                     /gene="rplR"
                     /locus_tag="PK839_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009597644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HOG71980.1"
                     /translation="MALSKEERRLRIRRRIRKKISGTAEKPRLTVFRSNTNIYVQFVD
                     DVTGRTLASASSMVKDIAGKPKITKTEQAKLVGQLAAKNALEKGITTVVFDRSGYLYH
                     GRVKALADAARESGLKF"
     gene            15109..15630
                     /gene="rpsE"
                     /locus_tag="PK839_04245"
     CDS             15109..15630
                     /gene="rpsE"
                     /locus_tag="PK839_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HOG71981.1"
                     /translation="MSANTNIHRVKASDLELKDRLVSIQRVTKVTKGGRTFSFSAIVV
                     VGNEDGVVGYGLGKASEVTAAIAKGIEDAKKNLVKIPVFKGTIPHEQEAKYGGAKVII
                     RPASQGTGVKAGGAMRAVLESVGITDVLAKSKGSSNPHNLVKATVMALLELRDARTIA
                     EQRGISLSKVFNG"
     gene            15643..15816
                     /gene="rpmD"
                     /locus_tag="PK839_04250"
     CDS             15643..15816
                     /gene="rpmD"
                     /locus_tag="PK839_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009161000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HOG71982.1"
                     /translation="MAKVKITQIKSVIGHPERQRRTLRSLGIKRMHQTVEVEGTPQVL
                     GMIAKVRHLIRVE"
     gene            15848..16294
                     /gene="rplO"
                     /locus_tag="PK839_04255"
     CDS             15848..16294
                     /gene="rplO"
                     /locus_tag="PK839_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018667878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HOG71983.1"
                     /translation="MNLNNLKPAKGSTRNRKRVGRGTGSGHGRTSGRGQKGAQSRSGH
                     SYKVGFEGGQMPLQRRMPKYGFKNPFRIEYQAINIGTLQALAEKNGYSIIDVDTLIEA
                     GLVTRNERIKILAKGELTAKLEVKAHAFSKKAIEAIESANGTAVKI"
     gene            16299..17615
                     /gene="secY"
                     /locus_tag="PK839_04260"
     CDS             16299..17615
                     /gene="secY"
                     /locus_tag="PK839_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HOG71984.1"
                     /translation="MKSFIQTLKNIFKIEDLRKRILYTLGILLVYRLGSFIVIPGLDP
                     DQLSALQEQTKEGIMGLLNMFSGGAFSNASIFALGIMPYISASIVVQLLGIAIPYFQR
                     LQKEGESGRNKLNQITRILTIAILILQAPAYLANLHAQLPESAFIVKGASFTIFATIV
                     LIAGTMFIMWLGEKITDKGIGNGISLIIMIGIIARLPFALVAEFFSRAEELGGGLVMF
                     LAELVVLFVVFMFTILLVQGTRKIPVQYAKRIVGNKQYGGVRQYIPLKVNAAGVMPII
                     FAQAIMFIPMLFARFDSTAGIAAAFNYTGFLYNFTFAILIILFTYFYTAIIINPNQMA
                     EDMKKNGGFIPGVKPGKKTVEFLDSIMSKITLPGSIFLAIIAILPAFAMILGVNNQFA
                     HFFGGTSLLILVGVVLDTLQQIESHLLMRHYDGLMKSGRVKGRAGY"
     gene            17643..18419
                     /gene="map"
                     /locus_tag="PK839_04265"
     CDS             17643..18419
                     /gene="map"
                     /locus_tag="PK839_04265"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HOG71985.1"
                     /translation="MLAIKTEEEIELLRLNNDLVSKTLAEVAKIVRPGITTKELDHRA
                     EEFIRDNGAKPGFLGYDGFPATLCVSVNDVVVHGIPSNRVLHEGDIVSVDCGTYMHGF
                     YGDSAYTFEVGDVAPQVKRLLRVTRECLEHGVEAAVEGARIGDVSHAIQTHAESNGYS
                     VVRELVGHGLGRDMHESPEVPNYGNKGRGPKLPAGLVICIEPMINLGTRYVYQDSDGW
                     AVRTQDGKPSAHYELAVVVRNGKAETLSTFQYVEQVITKS"
     gene            18432..18650
                     /gene="infA"
                     /locus_tag="PK839_04270"
     CDS             18432..18650
                     /gene="infA"
                     /locus_tag="PK839_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HOG71986.1"
                     /translation="MSKQPAIEKDGTIVEALSNAMFRVELDNGHVIIAHISGKMRMHY
                     IKILPGDKVRVEMSPYDLTKGRITFRYK"
     gene            18670..18786
                     /gene="ykgO"
                     /locus_tag="PK839_04275"
     CDS             18670..18786
                     /gene="ykgO"
                     /locus_tag="PK839_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014790280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HOG71987.1"
                     /translation="MKVRTSIKKRSDDCKIVRRKGRLYVINKKNPKFKQRQG"
     gene            18830..19207
                     /gene="rpsM"
                     /locus_tag="PK839_04280"
     CDS             18830..19207
                     /gene="rpsM"
                     /locus_tag="PK839_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009125149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HOG71988.1"
                     /translation="MARIVGVDLPKNKRGEVALTYIYGIGRSSSRRILDKAGISYDKK
                     VSEWDDNELNAIRTILNEEYKVEGELRSMVQMNIKRLMDIGCYRGIRHRLGLPVRGQS
                     TKNNARTRKGRKKTVANKKKATK"
     gene            19223..19612
                     /gene="rpsK"
                     /locus_tag="PK839_04285"
     CDS             19223..19612
                     /gene="rpsK"
                     /locus_tag="PK839_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HOG71989.1"
                     /translation="MANKRVSSKKKVVKVDPVGQAHIHSSFNNIIITLTNSTGEVISW
                     SSAGKMGFKGSKKNTPYAGQTAATDCAKVAYDMGLRKVKVFVKGPGAGRESAIRTINA
                     AGIEVTEIVDVTPLPHNGCRPPKRRRV"
     gene            19657..20265
                     /gene="rpsD"
                     /locus_tag="PK839_04290"
     CDS             19657..20265
                     /gene="rpsD"
                     /locus_tag="PK839_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018667875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HOG71990.1"
                     /translation="MARYTGPSTKIARKFGEPILGPDKYFEKKNYPPGMHGLMKKRKK
                     QSEYGVQLQEKQKAKYTYGLLERQFLNTFKKAARSKGITGEILLQLLESRLDNVVYRL
                     GIAPTRAAARQLVGHKHVTVNGSVVNIPSYHVKAGDIIGVREKSKSLEIISDSLAGSH
                     NTRYSWLEWNKDEMYGKFLNVPERSEIPENIKEQLIVELYSK"
     gene            20282..21274
                     /locus_tag="PK839_04295"
     CDS             20282..21274
                     /locus_tag="PK839_04295"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HOG71991.1"
                     /translation="MAILAFQKPDKVIMLESTGTTGTFEFRPLEPGYGITVGNALRRI
                     LLSSLEGYAITWIRIRGVDHEFSTIPGVMEDVTEIILNLKQIRFKRIVDGMDEEKVTI
                     TITGQNQFKAGYLSNFLSGFKVLNPELVICRMEPDVKIEVDVHIGKGRGYIPAEENKP
                     EDAEFGIIPIDSIHTPIRNVKYSVENYRVEQKTDYEKLVIEIDTDGSIHPKDALKEAA
                     KILIYHFMLFSDEKITLETEDKFETEEFDEEALHMRQMLKQKLVDLDLSVRALNCLKA
                     ADVETLGDLVRYNKNDLLKFRNFGKKSLAELEDLLDGLNLSFGMDISKYKLDKD"
     gene            21281..21814
                     /gene="rplQ"
                     /locus_tag="PK839_04300"
     CDS             21281..21814
                     /gene="rplQ"
                     /locus_tag="PK839_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005867127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HOG71992.1"
                     /translation="MRHNRKVNHLSRTSAHRKAMLSNMASSLILHKRINTTVAKAKAL
                     RIYVEPLITKSKNDTTHSRRVVFSYLQNKYAVTELFREVSQKVADRPGGYTRILKTGT
                     RLGDNADMCIIELVDYNTNYVKESAGKTEAKRTRRGGSRAKKEETPAVESPKSATSEV
                     NSEIPSEGEISEEKISE"
     gene            21904..23196
                     /gene="eno"
                     /locus_tag="PK839_04305"
     CDS             21904..23196
                     /gene="eno"
                     /locus_tag="PK839_04305"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOG71993.1"
                     /translation="MGQIVSIHAREILDSRGNPTVEVDVLTESGYFGRAAVPSGASTG
                     VHEAVELRDGDKGRYLGKGVLKAVNNVNTTISEEIVGMHVLDQQEIDEAMIKLDGTPN
                     KGNLGANAILGVSLAVAQAAAQMTTQPFFRYVGGVNARTLPIPMMNILNGGSHADNSI
                     DFQEFMIMPVGAESFSEAIRMGAEVFHNLKNVLKKLGYSTNVGDEGGFAPNLKSNEEA
                     VKVILQAIENAGYRPGEDVFIALDPASSEYYLPEENVYHLHKSSGDKLTPAQMVGYWK
                     DWVTRYPIISIEDGMAEDDWSGWKLMTDTLGSKIQLVGDDLFVTNVERLQKGIDEGVA
                     NSILVKVNQIGTLTETINAVRLADTHAMTAIISHRSGETEDTAIAHLAVALNTGLIKT
                     GSASRSDRIAKYNQLLRIEEMLGPSAIYPGRNFKYIKK"
     gene            23278..25176
                     /locus_tag="PK839_04310"
     CDS             23278..25176
                     /locus_tag="PK839_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis decarboxylase"
                     /protein_id="HOG71994.1"
                     /translation="MAYKSLHHFINKLEEQNELIRISAFVDPVLEIAEITDRMSKQPN
                     GGKALLFENTGTSFPVLTNALGSINRICLALGVSGLDDVGSDINKLFAELAGPKTNIW
                     EKLGTIPTLGKLSAWLPAQISSRGACQEVVHAEPNINLLPVMQCWPFDGGRFITLPMV
                     NTKDPFTGIRNVGMYRMQVFDPKHTGMHWHQHKVGARHFEAYKKLGKRMPVAVALGGD
                     PVYTYAATAPMPDNMDEYLLAGFLRKKKVKLVKCLTVDLEVPEDVDFVLEGFVDPTEE
                     LAWEGPFGDHTGFYSLADWYPRFHLTCITHRRDAVYPATLVGVPPMEDAYIAKATERI
                     FLQPLRITMLPELEDLALPVEGTAHNIALVKFRDYYPGQAYKIMNAIWGAGQMMFNKL
                     LVALPKDIDLTNADQILHSLVNFVDPERDLMFGKGPLDILDHATPTQAFGSKLFIDAT
                     NQTVVMPHLQISINDVKELLERETSRVKNFNLNLLNRGISILIISVDKQAVTNVKSYM
                     QQLFRCQELAGLRAMVAVDMGDAVTDLSMLTWFASSNIDPIRDVFILNLNDGYPARVG
                     VDGTRKAYPQDNFKREWPNVVAMSREVVDHVNKQWEKYGIGGMVESPSKQLLPLVAGE
                     GPVVKLNS"
     gene            25435..28725
                     /locus_tag="PK839_04315"
     CDS             25435..28725
                     /locus_tag="PK839_04315"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HOG71995.1"
                     /translation="MLLFFIKGGGELFAQKTEVEVFDANNGLPQTYVSCLIQDSKGFL
                     WLGTHSGLCLYDGYQFTTFSNNPLDSLSLCNNFVKSIAEDKNGDIWVATLSGLSRYER
                     ATGKFISYFSEPTNPASLAGDKIYYVYVDREKNLWVHTPGTLNLYSYKSNDFTRFNYF
                     NDLFTQPVETDIYPIYEDLQGRIWVGTKDGLMLFDRERMLFKRYSSRISYPYSIYGNR
                     VQAIYEDSRGNFWVGTDKGLNLFNFKLQRFYPVGAGIDENDAVRFIFEDSRSTLLLGM
                     ESGVARLHSSTEKVEQLQNFYSKSSKILPIGISAILKDKSGITWVASRTGLIKINPFS
                     QRFKGYSRDNLGNPLFSNNIIASVFEDSSGKLWVGTWGRGLFIFNPKTGENLRYCKSS
                     SIYRLPNDFAHTVFQTRKGEILVGTRNGVFVYDQRSRQFSNFFASRHVSVGNIFVDNR
                     VYSIDEDSAGNLWFATAIGLHCLSGNRLHSYYSRRGDSIFLPSNLIFDVLAGKQGEVW
                     VATANGLSRISDDCSSVINITVMTDSAGQGQQPLGEVYCLHQLSNGAILAGTSNGLVS
                     VNLLKGSKNLYFENLIIPSISARAIEEDVKGNFWLSTSKGITCYNPSTGQLASYTSDD
                     GLLGNEFNLLSSFQSPSGTIYFGGMQGISYFHPDSVMLNSHIPPLSFTKISLASVSST
                     KNIHPFGIDEVRIPQGFSSLTIEFSALDFNHPEKNLYRYRLQGPGSSKWVELGNKHSI
                     TLTNFREGVFRLEVMGANSDCVWTTEPLSLRIIVVAPFWRSRIAYTVYALLLLFVLIA
                     YGSYRRRNSLRIKRLLAEREDVMAKLELQKEELENKNKNITDSIHYAKRIQDAMLPTV
                     DMFKSHIPNSFILFKPKDIVSGDFYWVKETDSKCFVAVVDCTGHGVPGAFMSIIGIEL
                     LRNITEVEAVEDTAEVLNRLSASVHETFSSVSYPNESGLKVKDGMDVTFCTFDKQYDI
                     MQFTGAFSDLYIIRDGNIIEVKGDRFSVGMADDAGRYMFSSSYIPLMPDDMIYLFTDG
                     YVDQFGGPEGKKFKFRRFRRLLLNIYRLPLETQQKILEESITEWMGGQEQVDDILVIG
                     IRTPTKAK"
     gene            28972..31278
                     /locus_tag="PK839_04320"
     CDS             28972..31278
                     /locus_tag="PK839_04320"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOG71996.1"
                     /translation="MKRLAILLLFSILTPIQNFGQTVSLVLSGGGAKGCAHIGVIKAL
                     EENGIPIDNVSGTSIGAIVGGLYAMGYSPDEMVNIFKSRDFYNWSNGLIDESYKFNIN
                     DLSTTDAENLSVGFTFDRGGLKTKLTSSIVPPVGLDIAFEEMFAQGTAISRGNFDKLF
                     VPFRCNASDVVNKKQVYFREGNLGKAIRTSMTFPLYFTPVYIDSLLLFDGGIYNNFLW
                     KEALQEFHPNIIVGSKVASNSKLPSDEDPLLQVEAMIVGITDYSIPDSLGIVIDTRLE
                     NIELLDFDKVDEIVSAGYNETLKVIPKLREQITRNVTPEELAMRRKNFRQNLPPLFID
                     NIRVGNLSDRQKTYLHKVIFNEYTIVTFDKFKKDYYRLVSDRTFKRLIPEFEFDYTSG
                     LFDVALNPTLNRSVDLGLGLSLSSDVGNEGFASASYKWLSRTSNTIYGNIYFGKLLSS
                     IRLDYIKTFPTKIPVSLVACTIASRYDYHSSNPIPFFEDVKPPYVLQDETFATVSLKV
                     SNTSATTTTLSVSGGEKVDHYYQAMDYYSFDVPDQTTFKFIKGSLCFDKYTLDSKQYA
                     TRGRHQQLVISGFSGREEHYPGTTAPQMTGVRKNHSFATFYLYNESYHRIWRRRFWLE
                     LLFESYYSTQGLFSNYFASILTSNQFSPTPHSNLIFIENYRSTQYAAVGVAPIFEFWK
                     NVHFRVGAYLYQPFRTLKQDDVGVANYSDYFAKRWLIGSASLVYRTPVGPIALTLGYY
                     PTANSNGDDIFFNLTFGYSIFNSRVFDN"
     gene            complement(31290..33071)
                     /locus_tag="PK839_04325"
     CDS             complement(31290..33071)
                     /locus_tag="PK839_04325"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOG71997.1"
                     /translation="MKIAEKILTFFTLKIERKTARDSADRLVQLSIVTIPFLLYLVVF
                     CTLLLFRHQVASGIIAGVAAISILLIIVLAYSKKRVNSFGLVALAISGIALLAILIVG
                     KLDAALMIGVAMFPMLALSTTTPNRAHIISFIFWILYSCILITGPKFIGESVSSTGYY
                     RLLSIIFYPLLQLVASLLYLFKAKRLQELEKQLIEQKNQNKLREDYLAKLSHQLRTPL
                     NNIMVISNMLSNTITEDKYRDLIDTIQASTNSLAGVVNGMVEKSSTGIEPETDFAIPF
                     NLSDTINNTIRLFATQSQTVNFNIKIDESIPKTLKGDPVKLKQIFLNIIENVIKNKEG
                     SKVNIEIIASRMGNAKNSLELFFEVKSNVPLLIHQNEGYSQTVIADSTSSSLNNQFFI
                     DLLDLTITNNLVQSNGGKLNIKLTSNDAVVSFTYFLNSLPEIKKVAEPEVLKTTQRQH
                     DEISTLTAQPSAEAKGDGKLSNANVLLVEDNLINQKIVILSLKKLVKNIDVAANGKEA
                     LDKFGTSQYDIILMDVQMPIMNGYVATRKIREIEASTNSHTPIIAITANALLGDREES
                     LAAGMDDYISKPFQIEVLIQKMKKFLE"
     gene            complement(33450..34064)
                     /locus_tag="PK839_04330"
     CDS             complement(33450..34064)
                     /locus_tag="PK839_04330"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HOG71998.1"
                     /translation="MGLAASIPLGPVGVVCVQRTINKGRISGFFSGLGAATTDTIFAA
                     IGGFSLTFIIGFIEAKQQIIELAGGIIIILLGLRTFYTNPVTQLKRHKKKPNTLVEDF
                     ISMFLLTGANPFTIFLFIALFAALGIVTKNQNLLFTATALVGVFLGASLWWFTLSSLV
                     NLFRHKFRLKQLWWMNKITGSIIFILGIIAIVGVVNKAWFGQLP"
     gene            34329..35954
                     /gene="lnt"
                     /locus_tag="PK839_04335"
     CDS             34329..35954
                     /gene="lnt"
                     /locus_tag="PK839_04335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HOG71999.1"
                     /translation="MRKFYNPNFCRVFFSGILLALPWYEGLPSYSIFFGFVPLLLAYD
                     KLNEKGAGFWKLMLYAFIAFFTWNALSTWWIYKATMLGAILAVLITTMLMTLVFAGYH
                     FVRKYAGKVAGIFGFITLWIAYERFFHDSEVAWPWLSLGNSLGNHPKVIQWYEFTGMM
                     GGSLWILVINFLILGLVHAIRGKQIRRAGWFSIAALLTVILPLWISISMYNRYHEVQN
                     PVRVVIVQPSIDPYNEKFSGLTNEQQLDIMFSLAKQAADSTTDYVVCPETAIDDRIWE
                     HELHVNSSILRIKQFVDSLPNVKWVTGAITLKLFSSSENLPPTARELPYREGYFYDRY
                     NSAIQVDTAFQLPIYHKSKLVVGVEMMPYPKQLKFLEKLAIDLGGISGSLGTQPDRGV
                     LWSPDRRFGVGPIICYESDFGEFVSGYVKNGANLLFVITNDGWWGDTPGYKQHLSFSR
                     IRAIETRRSVARSANTGISAIINQRGDLVRTLGWWERGTISGTINANSKQTWYVREGD
                     YIGRLSAFMAVLTLAAALAAYLMKNNGRSVKRS"
     gene            complement(36159..38183)
                     /locus_tag="PK839_04340"
     CDS             complement(36159..38183)
                     /locus_tag="PK839_04340"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HOG72000.1"
                     /translation="MKRNIIQRGITMVLLTAGSISAFGQVEDPVQWSISSKKIDAKKY
                     ELIITATIEQGWHLYSTKLPEGGPLPTSFNFADSKDYKLVEGVKELTKPKTEHDDIFN
                     LDLSFFEGEAQFGQIVDLAGDEASINLTVEYQACYADKCIYLEKDLSHKLGKNGSVVL
                     AGEEVQPEKTTTATLAPTNDESAKKSSSSLWVFFFVSFAAGLAGLLTPCVFPMIPMTV
                     SFFMGKQGNRMNAILNAIVFGVSIIVIYTSLGLLVSLFNLGPDFTTTLTTHWITNLIF
                     FLLFIAFAASFFGLFEIVLPGSVANKTDSKADKGGFAGSFFMALTTVIVSLSCVGPIV
                     GALLVEAASGLGAKPIVGMFGFSLAFSIPFTLFAIFPSWLNSLPRSGGWMNSVKVVLG
                     FIVLAFSLKFLLTIDQSYNLNFLSRDIYLAIWIAIFTLLGLYLLGKIKFKFDSDLQHV
                     GVLRLFLAIASFTFVVYLIPGMFGAPLKAISGLIPPQSSQKFSLNQNSQAVMPTVNDN
                     TNLSLCDQPKYADIFKLPYNLKGYFDYNQALECARKLGKPIFVDFVGHTCANCKDMEA
                     RVWSDPRVLERLNSSYIIVALYVDDRTELPESEWVTSTRDGKVKKTIGKKNSDFQATR
                     FNVNGQPYYVLLGTNEQMLTEPMGYNTNVDEFVAFLDKGVEEFKKSTTVK"
     gene            38550..40064
                     /locus_tag="PK839_04345"
     CDS             38550..40064
                     /locus_tag="PK839_04345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HOG72001.1"
                     /translation="MKLFTTKQIRELDAYTIKHEPISSYELMERAANALYSWFAQNLS
                     TLQPIVVFCGPGNNGGDGLALARLLYTSGYSVEVYYLTASAHSSDFQLNLERLKAINF
                     LPVEIFSNSDFPHIGENQVVVDAIFGSGLSKPIEGLAAQLVSHINTSGSRVVAVDIPS
                     GLFGEENPFPNSNPVVRAADCLTLQFPKLSFFFAENSSFVGRWHVLPIGLHPEAIAGA
                     STSWHYTNMETIRGMVKPRERFAHKGIFGHCLVVAGSHGMIGAAVMAAMSAARAGAGL
                     VTLHVPACGYEIAQTLAPNIMVVCDSNEHHLSAVDDFQRYDAVCIGPGLGQHPETADA
                     LGKILAEINVPLILDADALNLVAANPKLLGAIPAGTIITPHPGEFDRLFGVSTCGWKR
                     LERAREVAQRYQHVIILKGAYSQVVLPDGRVFFNSTGNPGMATGGSGDVLSGIVVGFL
                     AQGFSIADAALLATFVHGLAGDIAASDLGENALVATDMISYISNALLEIEISNL"
     gene            40084..41181
                     /locus_tag="PK839_04350"
     CDS             40084..41181
                     /locus_tag="PK839_04350"
                     /inference="COORDINATES: protein motif:HMM:NF027441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4831 family protein"
                     /protein_id="HOG72002.1"
                     /translation="MRFSSMMVGLFLGLTMLSCSNKMATVSPVRSAPPVKDYSFIYTL
                     PRTVILIKVDACRSVFYPGPYAAYAWKYLGISDVPTLKSEQWTITDITIKGISESDPS
                     MLFAVFTDGHVPDALVQMTEAGLVIPENRAVIVSMMSKDLRRIENQAIPFSDLSVEPF
                     IGEEKNILYTKVQRDSSFVRVPIQKSMIVEKNADEKAGEAADLIFNLRKRRLEFLTTD
                     VDQPLEGNAVHVILDEIYRLENEYLSLFLGKTAVDTISKTYLFTPSGENNEKAIAFRF
                     SPTKGLLAQNDLSGSPMLVEVEPEPVPDSSEPLLATIGRLAEKQKLAQIFYRIPVVST
                     VKISDGKSEIASRRIPIYQLGPLVKIPAEFY"
     gene            complement(41198..41737)
                     /locus_tag="PK839_04355"
     CDS             complement(41198..41737)
                     /locus_tag="PK839_04355"
                     /inference="COORDINATES: protein motif:HMM:NF024498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HOG72003.1"
                     /translation="MRRFALLLAQLTLTAGVTIAQESSKVKWYTLEEALKLNTSEPRK
                     IFIDVYTDWCGWCKKMDSNTFNNPLIASYLTKDFYAVKFNAEGKNEITYKGQVYKNQG
                     EGQRSTHDFAIALLQGKLAYPSIAFMDGNGNLITFLSGYLTPSQIEPIMVYIMSDAYK
                     TQPWEQFASKFVSKLTSGE"
     gene            42104..>43561
                     /locus_tag="PK839_04360"
     CDS             42104..>43561
                     /locus_tag="PK839_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HOG72004.1"
                     /translation="MNKDIFKLEPQRLWKHFYSLTQIPRPSKKEKAAVDFMINFGRNL
                     GLETITDEVGNVIIRKPATPGMENRKTVILQGHLDMVPQKNSDKKHDFEKDPIEAYVD
                     GEWVTANGTTLGADNGIGVAAAMAVLEAKDIAHGPVEALFTIDEETGMTGAFGIKPGL
                     LKGDILLNLDSEDEGELYVGCAGGLDANIRFSFTPQPVPSDMVAYKLAVLGLKGGHSG
                     LEINLGRGNSIKLLFRFLYRACKTLGVRIGTIDGGSLRNAIPRESFAVVTVPSKNATN
                     FEKLVREYEQIYKNEFSLTEPNLSFTCKTDSLPKFLVDENSQSRAIRAFFSCPNGVVR
                     MSDAVPGLVETSNNLARVYTEGNQIVGQCLMRSSVDSAKEALADKMAAIFELADAAIL
                     FEGGYPGWKPNPNSPILTVMQQVYKNKFGTTPEVKAIHAGLECGLLGGVYPNLDMISF
                     GPTIRHPHSPDEKVNIATVGKFWEFLVEVLKNVPLK"
BASE COUNT        12275 a         9326 c        10155 g        11805 t
ORIGIN      
        1 gaaccgccgt gcccgccgca ggcggaagcc gggtggtgtg agagggtggg ggagtgtggg
       61 ggagtaatcc tcctacctac tcggttggaa ttatccgaat tggttttgct gccattgtcc
      121 tgttggtttt tttggcttaa ttacattagt ttaaccattt ttaaagcatg gcagttttag
      181 cttaaaatca aaatcaaaag caaaaaaaca ctacttttga ccggattaaa taatagttta
      241 acctatgctg gtattcatac actggaatgt aaatcctgaa atcttcagta tcgggaatct
      301 ctcggttcgg tactatggcc taatgtgggc gttggcattc tacctgggtt atgttatttt
      361 caaccgcttt gtgaaaaagg agggattgcc caacggattt ctcgactcgc ttaccatgta
      421 catgttgata ggaaccgttc ttggtgcccg acttggccac tgcctttttt acgaaccatc
      481 atactacctt agccatcctt tggaaatact taaaatatgg aaaggaggac tagccagcca
      541 tggggctgcc ctggggattc ttattgctct ttaccttttt gggcgaaagc ataaagtccc
      601 catgatttat gttgttgaca gggtggttat tactgttgcg ctgggtggcg cccttatacg
      661 gattggcaac ctgatgaact ccgagatata tggagtcaaa acaaacctcc cgtggggttt
      721 catttttgaa cggaacaacg agatataccc caagcaccca actcaacttt acgaggctat
      781 tagctatctg gccattttct tccttctgca ctacatttac cgtaaaaatg aaggtaagct
      841 taaaacaggt tttattttcg ggttattttt ggtgctcttg tttatggccc gcttcctaat
      901 tgagttcctc aaggaacccc aggttgactt tgaggcaaaa atgactttga acatgggtca
      961 gctgctgagc ttaccgttca ttgcagttgg cattgcattc atcatctact cgcaggtgaa
     1021 accttcctac tacacaccgc ctaagcaaac acctgtccaa aagtcgaaaa agtctaaagg
     1081 gtagaattgg gagttttatt cggttcattt acctggctgc aagtacttta aaaggtaaat
     1141 ttatacggtt ggaacaggag ttcaactttc ttctcaattc tttcagaagg ttacaagcta
     1201 ctttttgtaa gggtagttgt ggcatactat cctaaagctc tttagttagt ttcaaaaata
     1261 aagggcaacc ggatgttaac ccggtagccc tgataagttg gttggcttgt aaaagccaga
     1321 tttattcttc ttcttcttcg gcctcgtatg ctttcagcaa tttttcctga acatcagtcg
     1381 gaacctgctc gtatgaggca aacttcatgg tgtaggttgc ccttccgctg gttagggacg
     1441 acagaatggt ggagtacttg ttcaattctg ccaggggaac tttggcgtta atcacctcaa
     1501 aaccctttgt gcttcccatt cccatgataa ttgccctgcg gttctgcaga tcgctcatta
     1561 catcccccat gcggtcggaa ggaactagca cctccacatc gtagatgggc tccataattt
     1621 ttggcccggc ctccttaaaa gcattcttaa aggcatttct accagccagc ttaaatgaaa
     1681 tttcgttaga gtcaaccggg tgcattttgc catcgtaaac ggccacccgg atgtcgcgcg
     1741 catacgaacc ggtgagcggc ccttgttcca tcttttccat aatccccttt agaatggcag
     1801 gcataaagcg ggcatcaata gatccaccaa caatacagtt ataaaatatc agctttccgc
     1861 cccagtccag ctttatttcc tctttacccc taacggaaag gttaatttcc ttgccattta
     1921 ttttgtactt aacaggatcg ggggctccct ccacatgggg ttcaattacc aggtgaacct
     1981 caccaaactg acctgcacct cccgattgtt ttttatggcg gtagtcggct tgagcaattt
     2041 tggtgatggt ttcacggtag ggtattcgcg gagggataaa ctcaacttct agcttgttga
     2101 ggttagtaag ctgccattta aggatgttga ggtgatgctc accctgtccc gaaattatta
     2161 tctgcttaag ctcctttgaa tgttccactt caatggtcgg atcttcctgg tgcgcacggt
     2221 taagcagctc tccaagtttc tcaacctcgg cttcattctt tggttttatt gcggttcgga
     2281 attttggatt ggggaattgc atgggttcaa atgtgaaatc aatcccaggc ccgcagagcg
     2341 tttggttagt tttggaattc tttagcttaa cggtagcccc aatatcgcca gccaccattt
     2401 ccgatacctt tgtccgattt tttcctgcta cggcaaaaat ctgggtcaac cgttccttgg
     2461 tggagttgtt agcattggtc agatccattc cctcagcaat cttaccagac ataacccgga
     2521 agtagttgat ttcacctaga tgcggttcaa cagtagattt gaagatgaat agagatgttg
     2581 gcgcagcggc atcgcattta atttccgttt tgctgttgtt cttaagcatg ggtgtagaag
     2641 ccggggatgg gccaatctta atgataaact ccatgagtcg ttttgtcccg atgttctttt
     2701 tggccgacag gcagaaaacg gggaaaattt gacggttgcg aactcctata gatagaccct
     2761 tgcgcatttc ctcttcggaa agggaacctt gctcgaaaaa tttttccatt aaagtctcgt
     2821 cattttcggc agccgattct atgagggtat tgtgaagggc ggtagctttt tccatttcct
     2881 cggctggtat cgggaactct tcgcgaatgc ctgtatcatc cttaaactta tacatcttca
     2941 tggtgattac gtcaatgaag ctgtcaaata ggctcccggt gtttagcgga tactgggttt
     3001 gcagtacttt agtcccatag gtttgcctaa gcgaatccat tgcttgctca aagttggcct
     3061 tttcgtggtc gagctggtta actgctataa ggataggcct ttccaacctt tcggcatgac
     3121 ggttgaatat ctcggtacca acttccactc cgtactgggc gttgatggtt accaccacca
     3181 tatcggttgg atacattgag gtgataacgc ttccaacaaa atcgtccgac cccggtgtat
     3241 caattacgtt gagcttatgg tccatgtact cggtgtacag cactgttgaa aaaattgacc
     3301 tttggttgag ctgctcaatt tcggtgtagt ccgaaatggt attcttccct tcaatagaac
     3361 ctttccgctc aataactttc ccttcaaaca tcatggactc ggctagagac gtcttccccg
     3421 aaccggtatt tccaataagg gctatatttc taatttgatc tgttgagtaa gttttcatgc
     3481 gtaatatgct atttagtgtt tgacattctt ttctgctatc gtctcaatcc cagcacctac
     3541 tattatttat tttataaagg taatgtaaga aatacggctc tagtagcctt tggcgattgt
     3601 tgaatgcccc caaaaatagt aaaattttga aatatgcaag tttgttctgt gattctataa
     3661 aaaaatggtt gccttgcctc tttatcaagg gatacagtat aaaaatcaac cctgagcatt
     3721 ctttttttta ttcaaattaa gcagctgatt attagggtaa tgctactaat cctggccaat
     3781 tttattagct actgctttca aggttgaaga gtgccgatgg ctttttatgt taaaaaagta
     3841 tatctttgca cccgcaaaat tagttgtcgg caggtaaact tgctgaataa cagcaaaaaa
     3901 ataacaagtt gaacgatggt attgaaaaaa tcaatacctt tgcgcaccta ttttgtaaaa
     3961 ccaatttaac ataaactgta tttatgccca caattcagca gctagtaaga aaaggaaggt
     4021 ctaaaatggt ggataagagc aaggcgcctg cgctggatgc ttgtccccag agacgcgggg
     4081 tatgtgtacg ggtgtacacc actactccta aaaagccaaa ttcggctatg cgaaaagttg
     4141 ccagggttag gcttacaaac ggcaaggagg ttaacgccta cattcctgga gaaggccaca
     4201 acctgcaaga gcactccatt gtgctcattc gtggtggcag ggtaaaagat ttacctggag
     4261 ttcgttacca tctgatacgc ggaacactcg atgcttcagg agttgaaggg cgcaaacagc
     4321 gacgctcaaa gtacggcgcc aagcggccta agaaaaagta agtaatgtta acctcatttg
     4381 attatataca gcgatgagaa aatctaaacc aaaaaaaaga ttaatactgc ccgatcctaa
     4441 atttggcgac gtgatggtta ccaggtttgt taacaacctg atggagcgcg gcaagaaaag
     4501 cgttgcattc gacatcttct accaatcgct cgacatagta ggacaacgga tgaaagacgc
     4561 tgataagaac ccgcttgaaa tatggaagaa agccttggag aacatcaccc cacaggttga
     4621 agtgaaaagt cgcagggtag ggggggcaac tttccaggtt cctatggagg ttcgtcccga
     4681 gcgaaaaata tcgctttcca tgaaaaacct cattctttat gcccgtaaac gtagcggtaa
     4741 gggaatgagc gaaaagcttg ctgccgaaat tatggcggcc tacaacgagg aaggagctgc
     4801 atttaagagg aaagaggata tgcataaaat ggctgaggcc aacaaagctt ttgcccattt
     4861 ccgtttttaa caataccaca gcacgaggaa ggtaggaata tagatgagca gggatttaaa
     4921 atatactcgc aacataggaa taatggcgca cattgatgcc ggtaaaacaa ctactaccga
     4981 gcgcattctc ttttttaccg ggaaaaccca taaaattgga gaggtgcacg atggagccgc
     5041 cacaatggat tggatggtgc aggagcagga gcgtggaatt accattacct cggctgccac
     5101 tactacattt tggagataca acgaccagca gtaccaagta aacatcatcg atactcctgg
     5161 ccacgttgat tttactgtcg aggtggaacg ctcgcttcgg gttctcgacg gaacaattgc
     5221 cactttctgt gcagttggcg gtgttgaacc ccaaagcgag acggtgtggc ggcaagccga
     5281 caagtaccgg gttcccaaaa tcgcttttgt taataagatg gaccgtgttg gggcagattt
     5341 cctatctgtt gttggtcaga ttaaggagaa actcggggcc aacccagttc ctattcagct
     5401 gcctattggc gccgaggata cctttgttgg tattgttgac cttatcgaaa ataaggccta
     5461 tgtgtggaac gacgaccaaa aaagcaactc caacaatttt agggtagagg aagttcctga
     5521 ctccatgaag caggaggttg ccgaatggcg gagcaagctg gttgaagcag ttgcctccta
     5581 caacgatgac ttgctggagc gctttttcga caaccccgag tcaatttcta acgacgaaat
     5641 tatcgatacc attcggcggg caactatcga catgtccatt gttccggttc tctgtggtgc
     5701 cgcttttaag aacaaaggaa ttcaaaagtt gcttgatgct gttattgctt tcctgcctag
     5761 cccgctggat aaagggaata ttaagggtgt taatcccgaa accggaaatg aggagattcg
     5821 caagccatac atcaacgagc ctttctgcgg tttagctttc aaaattgcca ccgatcccta
     5881 tgttggccgt ctggccttta ttagagtcta ttcaggcaaa attgatagcg gctcctacgt
     5941 ttacaacgtt cgcacaggta acaaggaaag aatcagccgc ctttaccaga tgcatgccaa
     6001 caagcaaaat ccaattgagt tctgcgaggc cggagatatt tgtgctgctg taggatttaa
     6061 ggatattcgc acaggcgata ccctttgtgt cgagagcaat cccattgcgc tcgaatctat
     6121 gaccttcccc gaacctgtaa tcggactggc aattgagcct aaaactcaga aggacatgga
     6181 caaacttggt atagctctaa acaagctatc cgaggaggat cctaccttta gggttacatc
     6241 tgaccccgat agcgggcaaa ccattattag cgggatgggc gagcttcatc tggagattat
     6301 tgtagatagg ctcaggcgcg agttcaacgt ggacatcaac cagggtgcac cccaggttgc
     6361 ctatagggaa acgcttaccc gaactgtttc acaccgcgaa gtattcaaga agcaaaccgg
     6421 cggccgggga aaatttgccg atattatctt tgaaattggc cctgcagacg aaggcgtgca
     6481 agggttgcag tttattaacg aggttaaggg cggtaacatt cccaaggagt atgttccttc
     6541 catccagagc ggatttgatc aggccatgaa caacggcgtt ttggcggggt ttgcccttac
     6601 caacctaaag gttgtgctta aggatggttc tttccatccg gttgattccg acgccctttc
     6661 gttcgaaatt gccgcacgtt acggctttag agctgctgct ccaaaagcca gccctgtgtt
     6721 gctcgaacct attatggcag ttgaggtggt aacacccgaa gagtacatgg gtgatgttat
     6781 tggcgacctg aataaacgac gcgggcagat aaccggtatg gagtcaaagg gagttgccag
     6841 agtggtgaaa gccaaagtgc ctctttcgga gcagtttggc tatgtaaccg tgctgcgaac
     6901 ccttacatcg ggtcgggcta cttcaaccat ggaattctcg aactatgccg aggttccagc
     6961 tagcattgcc agcgaaatta ttgaaaaatc acggggcaag gtggttaagg aaattgaata
     7021 attactttta ccaatatgta caatgagcca gaaaattaga attaaactca aatcgtacga
     7081 ccacaacctg gttgacaagt cggccgaaaa aatcgtgaaa acggttaagg ctacaggcgc
     7141 tgtggtaagc ggtcctattc cgcttccaac acataagcgg atattcaccg tgaatcgttc
     7201 cacatttgtg aacaaaaaat cgagggagca gtttgagcta agctcctata agagattact
     7261 cgacatctac agctccaccc agaagacaat cgatgcgctg atgaagctcg aacttccaag
     7321 tggagtcgaa gtagaaatta aagtatgagt gtaacctgta aattgtaaaa aacaatgcca
     7381 caaggattaa ttggacgtaa aattggaatg acttccgttt tcgttgagga tgggaaaaat
     7441 gtcccctgca ccgttattga agcgggtcct tgcgttgtta ctcaggtaaa aactgtcgaa
     7501 tctgatggtt atactgcggt gcaactagct tttgacgaga aaaaggaaaa gcagactact
     7561 gccccaatga tggggcattt taagaaggca aacactacac ccaagcgtaa gctcgccgag
     7621 ttcagggact ggagcggcga aatgcagcta ggcgatgtga ttacagttga tatctttaat
     7681 accgatagat gggttgatgt aaccggaatt accaagggta gaggatttca aggagtggta
     7741 aagcgtcatg gatttggtgg tgttggtggg caaacccacg gccagcacaa ccggcaacgc
     7801 cacccaggtt caatgggtgc ttcatcctac ccgtcacgtg tgtttcctgg caagagactg
     7861 gcaggtagaa tgggcggcga cagggttaaa attatcaacc tacgcgtgct taaagtaatc
     7921 cctgaaagca acctactcct cgttaagggt tctattccgg gagcaaaggg ttcttactta
     7981 attattgaga agtaatggaa gcaaaagtac ttagcatatc aggacaagat acgggcagaa
     8041 ctgtccagct ggacgattca atttttgcca tcgagcctaa cgatcatgcc atttacctgg
     8101 atgtgaagca gattcttgcc aataaacgac agggaactca caaatcaaaa gaacgtagcg
     8161 aggtttctgg tagcaccagg aagctgaaac gtcaaaaggg aaccggaact gcccgtgcag
     8221 gtagcatcaa atcgccccta tttagaggtg ggggtagagt ttttggcccc gtcccacgcg
     8281 actacagctt taagctgaac aagaaagtga agctgcttgc ccggaaaagc gccctaacct
     8341 ataaggtaaa ggagaatgct tttatcgttg tggaggactt taatatcgaa accccaaaaa
     8401 caaaggagtt catcactatt tgtaaaaacc tcaacgtagc cgacaaaaaa accatattcg
     8461 tgttgagcga aacgaataaa aatatatatt tgtcgtccag aaatttacaa cagcagaagg
     8521 tcgttcaagc ctcatcttta aacacttacg aaatactcca tgcaaaagcc atggttcttt
     8581 gcgaaagttc agttgaggtg ctgcacaaaa tgtttaaaat ttcataaacc aagtggagta
     8641 tggacatact aattaggcct attgttaccg aaaagatgga gcgtttaacc ggaaagttaa
     8701 accagtacgg ctttattgtt gacaaaaggg caaataaatt tcagataaag gacgcaatcc
     8761 aggatatgta tggcgttact gttgaccggg taaatactat gcgctatgcc ggtaaacgga
     8821 agtcgcgtta cacccgtaca ggtgtagtta ccggaagaac caacagctac aaaaaagcgg
     8881 tggttacctt aaaggctgga gataaaattg atttttatag caacatttaa acgaaatggc
     8941 tgtacgcaaa ctaaaacctg ttacaccagg acagaggcac aaaattatag gcacattcga
     9001 ggaaattacc acatcgaagc ctgagaaatc gttgctacga cccctgaaga aatctggtgg
     9061 gagaaacaac caggggaaaa tgaccatgcg ctacataggt ggtggtcaca agcgtagata
     9121 ccgcttgata gattttcaac gcagtcgtga agacgaacct gcagttgtaa aatcggtgga
     9181 gtacgatcca aaccgcactg cccgcatcgc gctggtggag tatccggatg gcgaaaaacg
     9241 ctacattgtt gcccccaacg gtattaaggt gggccaaacg gttatgtcgg gtcatggcgt
     9301 tgcccctgaa gttggtaata cattatacct gtccgaaata ccacttggaa cgctcataca
     9361 caacattgag ctctaccctg gaagaggcgc ctgcatggcc cgaagcgctg gctcatacgc
     9421 ccagctcctt gccagagagg gtaagtacgt ggttgttaag ctgcctagtg gcgaaacacg
     9481 tatggttctc gcaacctgca aagctaccat tggaacggtt tccaaccccg atcacgcact
     9541 cgaaaaatca ggaaaagctg gtcgctccag gtggctcgga cgtcgtcccc gaaatcgtgg
     9601 ggtggctatg aaccccgttg atcacccaat gggtggtggt gagggaaaag cctccggagg
     9661 tcatccccgc tcacgcaagg gaatccccgc caagggctat aaaacccgct ctaagaagaa
     9721 ggcaagcaac aagtttattg tggaacgccg gaaaaagtaa tctgaataaa acaacgctat
     9781 tatgagtagg tcgctaaaaa aaggtccctt catagattac aagctggaga agcgtgtcat
     9841 cgagatgaac agcacttcca agaaatctgt tattaaaacc tggtcgcgta gttccatgat
     9901 ttctcccgac tttgtgggac acaccatagc cgttcacaat ggtaacaaat ttattcccgt
     9961 gtacatcacc gaaaacatgg taggacacaa gctgggtgaa tttgcaccaa cacgaacctt
    10021 ccgtggacac gcaggtaata ggaaaaagta agtgaattca ctaaattgat attaagcaat
    10081 gggtgcaaga aaacgtgaaa tggccaatag gcttaaagaa gagagaaagg acaaggcgat
    10141 ggctgttctt cgcaactgcc cgtcctctcc ccgcaaaatg cgcttggtgg tggatcagat
    10201 acgtggaaaa gatgtaaata cagcccttaa catactgaag ttcagcccaa aacatgcctc
    10261 aaaggatgtg cataagctgc tcctttcggc aattgccaac tggaagtcga agaatgaagg
    10321 catacgcctg gaggatgcca acctctttgt taaggagata ttcgttgacc agggaagaac
    10381 cctgaaaaga attagaactg caccacaggg acgcgcacac cgtattcgta agcgctctaa
    10441 tcacgttacc gtgatagtag atagtgctcc agcaattaac gatactaaca actaacagct
    10501 acatgggaca gaaagttaat ccaatatcaa acaggcttgg aatcataaag ggttgggatt
    10561 caaactggta tgggggtaaa aactactctc aaaagctagt tgaagactat aaaattcgcg
    10621 aatacctaaa cgagaggctc tccaaggcca gcctttcaaa aattgtaatt gagcgcaccc
    10681 tgaagctgat taccgttacc attcacactg cccgtccggg aatcatcatt ggaaaaggtg
    10741 gccaggaggt tgacaagttg aaggaggagc ttcgtaaaat taccaacaag gaggtgcaga
    10801 tcaacatatt tgaggtaaaa cgccccgacc tggatgcagt gatagtaagc aacaatattg
    10861 cacggcagct cgagggaaga atttccttcc gcagagccgt taaaaccgct atagcatcaa
    10921 ccatgagaat gggagctgag gggataaagg ttcaagtatc gggtcggttg ggtggagctg
    10981 aaatggcccg ttccgaaacc tacaaggagg gaagaatacc cctacatacc tttcgtgccg
    11041 acatagatta cgcgttggcc gaagcccaca ccaaggtagg tctaattggt atcaaggtgt
    11101 ggatatgcaa cgggctggtt tacggtaaaa gagacctctc gccaaacctt ggtatttcat
    11161 ctgccgcacc tgcccaatcg gggcatggac aagccaggcc tcatggtgct cgtaaaaaga
    11221 aaagtaataa gtagaacacc ttaaagcatt ctggaaatgt tacaaccaaa aaagaccaag
    11281 tatagaaggc agcaaaaggg tagaatgaag ggcaatgccc aacggggaaa ccagctggca
    11341 ttcggttcat tcgggattaa ggctttggat tcggcttgga ttaccagccg acagattgaa
    11401 gctgcccgtc aggctcttac ccgtcacatg aagcgtgagg gacaaatatg gattcgcata
    11461 ttccccgata aacccataac aaaaaaaccg gctgaagttc gaatgggaaa gggaaaagga
    11521 gcacctgagg gttttgtggc tgtggtaacc cccggccgca tgcttttcga gtgtgagggg
    11581 gtttcgcttg atattgccaa ggaagctctc cgcctggccg ctcaaaaact tcccattgct
    11641 actaaattcg tagtacggaa tgattatgtt gaacctgtaa gctagctaga acatgaaagc
    11701 atcagaagta agagaactta ccgataaaga gattgttgaa cgaatcgaag cagaaaagac
    11761 caacctcctt aagctcaagt taaaccatag catttcaccg ctggacaacc ccatgaagat
    11821 taaggaatcg cgtcgtacta ttgcccggtt gaaaacaatc ttaacccaac ggaagctaag
    11881 tcagaaccag taatgttgaa ttgcaatgga agaagttaag cgaaatttac gaaaggtacg
    11941 aacaggtgtt gtggttagca ataagatgga caagtctatt gtagttgctg ttcaccgcaa
    12001 agtaaaacat cctacatacg gaaagtacgt gaaaaaaacc accaaatttt acgcccatga
    12061 tgaggcaaat acctgcaaca taggcgatgt ggtgaagata atggaaaccc gtcctctcag
    12121 caagctgaaa cgctggagat tagttgaaat aattgaaaga gcgaagtagc catgatacag
    12181 caggaaacac gattaaaagt agccgataac agtggagcca aagaggtttt gtgcattcgc
    12241 gttctcggtg gtaccggcat gcgctatgcc cggattggcg atgtgattgt cgttaccgtg
    12301 aaaaatgcca ttccttctgg cgaaattaag aagggtaccg tttcaaaagc agtagtcgtt
    12361 agaactaaaa aggaaatacg caggcccgac ggctcctata tccgtttcga tgataacgcc
    12421 tgtgtgctgc ttaatgcaca aggcgaaatt cgcggaaccc gcatatttgg tcccgtggcc
    12481 agggagctcc gggataacta catgaaaatt gtatcactag cacccgaggt gctgtaatcc
    12541 cttaaaattc aaattacata tggcaagcaa gctacatata aagaaagggg accttgttta
    12601 cgtcatttct ggggaaagca agggcaagca gggcagagtg cttagcgttc aggtaagcaa
    12661 acagcgcgcc tttgttgaag gggttaacat ggtgtccaag cataccaagc ccaactccaa
    12721 gtaccctcag ggtggaatta tcaagatgga agccccaatt catatatcca acctgatgct
    12781 gattgatcct gcatcaggta agcctacccg cattggccgc aggcttaacg ataagggaaa
    12841 gctggttaga tactcaaaaa aatcaggaga ggagattaag taatggaata cgttgccaat
    12901 ttcaaaagga agtataagga ggaaattgtt cccgctttga tgaaggagtt tggatacaaa
    12961 agcgtgatgc aggttccccg gctggaaaag attgtcatca accagggcgt tggtcaggct
    13021 gtttccgata ggaaaattat tgaggcagcc caggccgatc ttacccttat agctggacaa
    13081 aaagctgtgc aaactttttc gcggaaggat atttcaaact ttaagcttcg taagaacgtt
    13141 cctataggtc ttaaagttac ccttcgtcgc gacagaatgt acgagtttat ggaacgtctg
    13201 atcaacgttt cgcttccccg gatacgcgat ttcaagggta ttaacgataa gctcgacgga
    13261 cggggtaact acaccctggg cattgaggag caaataattt tccctgaaat cgacatggat
    13321 aaaattaaca ggttgctggg catggagata acttttgtta cctccaccaa caatgacgag
    13381 gaggcatatg cccttctgcg tgaattcggt ttacccttta aaaatgctaa aaagaactag
    13441 tatatggcaa aagagtcgat gaaagcccgt gaggtgaagc gagcaaaact tgttgagaaa
    13501 tacgcagaga aacgtgcaaa gcttaaagca gaaggcgatt ttattggtct tcagaagtta
    13561 ccccgtaact ctaatcctat tcgccttcat aaccgctgca tgattacggg caggccccgc
    13621 gggtacatgc gtcagtttgg cgttagcaga atcacattca gggaaatggc ttcaaaaggg
    13681 cttattcccg gcataaagaa agcaagctgg taaaacacaa cataatttta gtttacaatg
    13741 attaccgacc ccatagcaga ttacctcacc cgaattcgta acgccgttat ggccaaccat
    13801 cgggtggttg aaatacccgc ttcaaaaatg aagctggaaa tgagccgaat tcttttcgaa
    13861 aaaggctaca ttttgaacta taagtttgag gatgacggca aacagggtac tatcaaaatt
    13921 gccctgaagt accatcctga aactaaaaag aatgctatta agcacattga aagagttagc
    13981 agaccaggat tacgtaagta cactaatgcc agagaactac cccgggtgct caacgggtta
    14041 ggtgtggcca ttatcagcac atcccagggc attatgaccg acaaggaggc ccgcgttaaa
    14101 aatattgggg gcgaagtggt atgctacgtt tactaaataa ttttactaac caaatacaat
    14161 ttttctaatg tctaggatcg gaaataaacc tgtaaaccta ccacagggtg taagcgttaa
    14221 aattagcgcc gataatgtgg taagcgtaaa aggccctctg ggagagctct cccagaaagt
    14281 tgacccggac atcaccgtca acttacaggg aaacctagtt gttctcacca gaccaaccga
    14341 tcaaaaacgc catcgatccc ttcatggact ttaccgagcc ctgattaaca acatggtgat
    14401 tggtgtttca acgggttggg agcttcaaca ggagttgatt ggtgttgggt acaaggccga
    14461 agccaaaggg cagctgcttc agctgagcct cggcttttcg catgacatcg atttcctcct
    14521 tccaaacgag gtgaaggttg aaaccaaaac cgaacgtaag ggaaacccta caatcaccct
    14581 gaagagcatt gacaagcagc tgttaggaca ggtagctgcc aagatacgct ctctccgtaa
    14641 acctgaaccc tacaaaggaa agggtattcg ttttgttggc gaagttgttc gtagaaaagc
    14701 cggtaagtcg gcaagtgtgt aacctgttaa tctattttga gatatggctt taagtaaaga
    14761 ggaaagaagg cttcgtataa ggagacgaat tcggaagaaa atttcgggta cggctgaaaa
    14821 accacgatta acggttttta ggagtaatac caatatttat gttcagtttg tggatgacgt
    14881 taccggtcga actttagcca gcgcatcgtc gatggtaaag gatattgctg ggaaacctaa
    14941 aattaccaaa accgaacagg ccaaattggt tgggcagctg gctgcaaaga atgccctgga
    15001 aaagggaatt accaccgtgg tgtttgaccg cagtggctac ctataccatg gtagggttaa
    15061 ggcattagcc gatgcagctc gggaatcagg acttaaattc taacgataat gtcagcaaac
    15121 actaacatac atagggtaaa agccagcgac ctggagttaa aggatcggct ggtaagcata
    15181 cagcgtgtaa ccaaggttac caaaggtggc cggactttca gcttttcagc aattgtagta
    15241 gttggcaacg aggatggcgt tgtgggttac ggtttgggta aagccagtga agttactgcc
    15301 gccattgcca agggtattga agatgccaag aaaaacctgg ttaaaatacc ggttttcaag
    15361 ggtactattc cccacgagca ggaagccaag tacggtggtg ccaaggttat catcagacct
    15421 gcctcccagg gaacaggggt taaagcggga ggcgccatgc gcgccgtgct ggagagcgtt
    15481 ggaattaccg atgtgctggc aaaatctaaa ggctcatcta accctcacaa cttggttaaa
    15541 gctacggtaa tggcacttct cgagcttcgt gatgccagaa ctattgccga gcaacggggt
    15601 atctcattaa gcaaagtttt taacggttag ttttttaaag ccatggcaaa agttaaaatt
    15661 acccaaatca agagcgttat tggccaccca gaacgccaac ggcgtaccct caggtcgctg
    15721 ggcattaaaa gaatgcatca aaccgttgaa gtagaaggta ctccacaggt attgggcatg
    15781 atagccaagg taagacacct tatacgggtg gagtagtacc tacagaacct taaaaatgga
    15841 taaaactatg aatctaaata acttaaaacc ggccaaggga tctacccgaa atcggaaaag
    15901 ggttggacgt ggaacgggat ccgggcacgg cagaaccagc ggacgcggtc aaaagggagc
    15961 acaatcgcgt tcgggccatt cctacaaggt tggtttcgag ggtggccaga tgcccctgca
    16021 gcgacgaatg cctaaatatg gttttaaaaa tccattcagg attgaatacc aggccattaa
    16081 catcgggaca ctacaggcgc tggctgagaa aaatggatat tcaattattg acgtggatac
    16141 cctgattgaa gcagggttgg ttacccgtaa cgagcgtatc aaaattcttg ctaagggcga
    16201 acttactgca aagctagagg ttaaagccca cgcattttct aaaaaagcaa tcgaggctat
    16261 cgaatcagcc aacggaacgg ctgttaaaat ttaaatggat gaaatccttt attcaaacac
    16321 ttaagaatat tttcaagatt gaggaccttc gaaagaggat cctctatact cttggaattc
    16381 tcttggttta caggcttggt agttttattg taattccagg gttagaccct gaccagcttt
    16441 ctgctctgca ggaacaaacc aaggaaggaa tcatggggct tctgaacatg ttctcgggtg
    16501 gtgctttttc taatgcatcc atatttgccc tgggtattat gccctacatt tcagcctcca
    16561 ttgtggtgca gctacttgga attgcaatac cctactttca acggttacag aaggagggag
    16621 aaagcggtcg taacaagcta aaccaaatta cccgaattct taccattgct attcttattc
    16681 ttcaggctcc tgcctacctg gcaaaccttc atgcccagct tcctgagtct gcctttattg
    16741 ttaaaggtgc tagctttacc atatttgcta ccattgtgct gattgccgga accatgttta
    16801 tcatgtggct tggcgaaaag attaccgata agggtattgg caacgggatt tcacttataa
    16861 tcatgattgg gattattgcc cgacttcctt tcgcactggt tgctgagttc ttctcaaggg
    16921 ccgaggagct tggtggaggt ttggttatgt tccttgccga gctggttgtg ctttttgttg
    16981 tattcatgtt tacaatcttg cttgttcagg gaacccgtaa aattcctgtt cagtatgcca
    17041 aacgaattgt tggcaacaag cagtacggtg gcgttagaca atacattccc cttaaggtga
    17101 acgcagcagg cgttatgcct ataatctttg cccaggccat catgttcatt cccatgctgt
    17161 ttgcccggtt tgactccact gcaggaattg ctgctgcttt taactatacc ggcttccttt
    17221 acaactttac ctttgcaatt ttaatcatcc ttttcaccta tttctacacg gcaattatca
    17281 tcaatcccaa ccagatggct gaggatatga aaaaaaatgg tggatttatt ccaggcgtta
    17341 aaccaggaaa gaaaacagtg gagtttttgg attccatcat gtccaagatt acccttccgg
    17401 gttcaatttt cctggccata atagccattc ttcctgcatt tgcgatgata cttggggtaa
    17461 acaaccagtt tgcacacttt tttgggggca catcgctgtt aattcttgta ggagttgttc
    17521 tggatacact acagcaaatt gaaagccatt tgctgatgcg acactacgac ggactgatga
    17581 agtctggtcg ggttaaggga agggcaggtt actagttgat gcctgcagct aagttcttta
    17641 caatgcttgc aattaaaacg gaagaggaaa ttgagctgct aaggcttaat aacgatttgg
    17701 tttccaaaac gctagctgaa gtagccaaaa tagttcggcc tggcattacc accaaggagt
    17761 tggaccatag agcggaagag tttatacgtg ataatggcgc aaagcctggc tttctcggct
    17821 acgacgggtt tccggcaact ctttgtgtat ccgttaacga tgtggtggtt catggaattc
    17881 cttctaaccg ggtgctgcac gagggagata ttgtttcggt tgactgcgga acctacatgc
    17941 atggattcta tggcgattcg gcctatacat ttgaagttgg tgatgtggct ccacaggtta
    18001 aacggttgct cagggttacc cgcgagtgcc tggagcacgg tgtagaagca gcggttgagg
    18061 gtgcccgcat aggcgatgtt tcacatgcga ttcaaaccca tgccgagagt aatggctact
    18121 ccgtggttcg ggagctggtt ggtcatggcc ttgggcgcga catgcacgaa agccccgagg
    18181 ttcccaatta tggcaataag gggcgcggac ctaaacttcc ggcaggtttg gttatatgta
    18241 ttgagccaat gattaacctg ggaacaaggt atgtttacca ggatagtgat gggtgggcag
    18301 ttcgaacgca ggatggaaaa ccatctgcac actacgaact tgccgtagta gtgaggaatg
    18361 gaaaagccga aaccctctcc actttccagt acgttgaaca ggtaattact aaaagttaat
    18421 aaggcgagcg gatgtcgaaa caaccggcaa tagaaaaaga tggaaccata gttgaagcat
    18481 tgtcaaacgc aatgtttcgg gtggagcttg acaacggcca tgttattatt gcgcatatat
    18541 cgggtaaaat gcgcatgcac tacatcaaaa tactacccgg cgataaggtt agagtggaga
    18601 tgtcgcccta tgatttaacc aaaggacgca taacatttag gtataagtaa acttaaatta
    18661 tcaaaggaga tgaaagtaag aacctcaatc aagaagcgca gcgacgattg taaaatagtc
    18721 aggcgaaaag gccgcttgta cgtgattaat aaaaaaaatc ctaagttcaa gcagcgtcaa
    18781 ggataaattg tttaattttg cagtctaaaa attttttaag aaatcaggta tggcacgaat
    18841 agttggtgtt gatttgccga aaaataagcg tggtgaagtt gcgctaacct acatttacgg
    18901 gataggccga agcagctcgc gcaggattct tgataaggcg ggaattagct acgataagaa
    18961 ggtgtccgaa tgggatgaca atgagctcaa cgccattaga accattttga acgaagagta
    19021 taaagtagaa ggcgagcttc gctcaatggt gcagatgaac attaaacgct taatggacat
    19081 tggttgctac cgtggaattc gccaccgtct tggtttgcct gttagaggcc agtcaaccaa
    19141 gaataacgcc cgcactcgaa agggccgtaa aaaaactgtt gccaacaaga agaaagcaac
    19201 caaataatat tgtgtacaaa ctatggcaaa caagagagta tcttcaaaga agaaagttgt
    19261 taaggttgat ccggttggac aggcacacat acattcttca ttcaacaata ttattattac
    19321 gctgacaaac agcacaggag aagtaatttc ctggtcatcg gcagggaaaa tgggatttaa
    19381 gggctcaaag aaaaacaccc cctatgccgg ccaaactgct gctaccgatt gtgctaaggt
    19441 agcctatgac atggggctgc gtaaagttaa ggtatttgta aaaggacccg gagcaggtcg
    19501 tgaatcagct atacgaacta ttaacgctgc tggcattgag gtaaccgaaa tcgtagatgt
    19561 tactccttta ccccataacg gctgccggcc tccaaaacgc cgcagggtat aattctttta
    19621 ctgaacggaa ttacagaaat aaataatttt aaaggaatgg caagatatac aggacccagc
    19681 acgaaaattg cccgtaaatt cggggaaccc attttaggac cggataagta ttttgaaaag
    19741 aaaaattatc ctcccggtat gcatgggtta atgaaaaaaa ggaaaaagca atctgaatac
    19801 ggagttcaac ttcaggagaa acaaaaagcg aagtacacct atggactttt agagcgtcaa
    19861 tttttgaata catttaaaaa ggctgcacgc tcaaaaggta ttaccggtga gattctactt
    19921 caacttctcg aatcacggct cgacaatgta gtttacaggt tgggtatagc tcctaccagg
    19981 gcagctgctc gccagctggt tggccacaag cacgttaccg tgaatggatc ggtggttaat
    20041 atcccctcct accacgttaa ggctggagat ataataggtg ttcgcgaaaa gtccaagtcg
    20101 ctcgagatta tttccgattc gctggcaggt agtcataaca cccgatactc atggctggag
    20161 tggaataagg acgagatgta cggaaaattt cttaatgtcc cagaacggtc tgaaattcct
    20221 gagaatatta aggaacagct aattgtagaa ctttactcta agtaatctaa cccataccat
    20281 aatggcaata ctggcatttc aaaaacccga taaggttata atgctcgaat ctaccggcac
    20341 aaccggtact tttgagttcc gtcctcttga gccaggctat ggtatcacgg tgggcaacgc
    20401 tttaaggcga attttgcttt cctcgctcga agggtacgcc atcacctgga tacgaattcg
    20461 tggtgttgac cacgagtttt caaccatacc aggtgttatg gaggatgtta ccgaaattat
    20521 tctcaacctg aagcaaatac gctttaagcg tattgtagat ggaatggatg aggaaaaggt
    20581 aaccatcacc atcaccgggc aaaaccagtt caaagctgga tacctttcca actttttatc
    20641 aggttttaag gtgttaaacc cggagctggt aatctgcaga atggagccag atgtgaaaat
    20701 cgaagtagat gttcacattg gtaaaggacg tggatacata cctgccgagg agaataaacc
    20761 tgaggatgct gaattcggca ttatccccat cgactccatt cacaccccca ttcgcaatgt
    20821 taagtattcc gttgagaatt acagggttga acaaaagact gactacgaga agcttgttat
    20881 cgagattgat accgacggtt ctattcaccc caaggatgca ttaaaggagg ctgccaaaat
    20941 ccttatttac cacttcatgc tcttctccga cgagaaaatt accctcgaaa ccgaggataa
    21001 gtttgaaacc gaagagtttg atgaggaggc tctccacatg cgtcaaatgc tgaagcagaa
    21061 gctggttgac ctcgaccttt ccgttcgtgc actcaactgt cttaaagctg ctgatgttga
    21121 aactcttggc gaccttgtca ggtacaataa aaatgacctg ctcaagttta gaaacttcgg
    21181 caagaaatca cttgccgagc ttgaagacct gctcgacggg ctcaacctat catttggtat
    21241 ggatatttct aagtataaac tggataagga ttaaatcgcg atgagacaca acaggaaagt
    21301 aaaccattta agccgtacaa gcgctcaccg taaagcaatg ctttcaaata tggcctcctc
    21361 gttgatactg cataagcgca tcaacactac tgtggccaaa gccaaagctt taagaatcta
    21421 tgttgagccc ctgataacaa aaagtaaaaa cgataccact cattccagac gcgttgtttt
    21481 cagctacctg cagaataagt acgctgtaac tgaacttttt agggaggttt cgcaaaaggt
    21541 agccgataga cccggaggat acacccgtat tcttaaaacc ggaacccggc tgggcgataa
    21601 tgccgatatg tgcattattg agctggttga ctataacacc aattatgtta aagaatcagc
    21661 aggtaaaacg gaagccaaaa gaacccgtag gggtggatca agagctaaaa aggaggaaac
    21721 acctgcggtt gaatccccta aatcagctac tagcgaagtt aattccgaga taccttcgga
    21781 aggagaaatt tctgaagaaa aaatatcaga gtagcgaagc tagcaacata ctatcagggt
    21841 gggggatgag cagcttgctt tatccccttt tttatctaaa tcatcaactt aaaaaaataa
    21901 actatgggac agattgttag tattcacgca agggaaattc tcgattccag aggaaatcct
    21961 actgttgagg tggatgtttt aaccgaaagt ggttactttg gtagagccgc tgtgcctagt
    22021 ggcgcatcta caggtgttca cgaggcagta gagcttcgtg atggtgacaa aggacgatat
    22081 cttggcaaag gcgtgcttaa agccgttaac aatgtgaata caaccatttc ggaagaaatt
    22141 gtcggcatgc acgtgttgga ccagcaggaa attgacgagg ccatgataaa gctcgatgga
    22201 actcccaaca agggaaacct cggggccaac gcaattctcg gagtatccct ggccgttgct
    22261 caggctgccg cccagatgac cacacagccc tttttcagat atgtgggcgg tgtaaatgcc
    22321 agaacattgc ccattccgat gatgaatatc cttaacggcg gatctcatgc cgataatagc
    22381 attgattttc aggagttcat gatcatgccg gttggggctg agtcctttag cgaggctatt
    22441 cgcatgggtg ccgaagtttt tcataacctg aaaaacgtgc taaaaaaact tggatattca
    22501 accaatgttg gcgacgaagg tggatttgca cccaacctaa aatcgaacga ggaagctgta
    22561 aaggtaattt tgcaagctat cgaaaatgca ggttaccgcc cgggtgagga tgtttttatt
    22621 gcactcgacc cggcctcctc agagtactac ctgcccgaag aaaatgttta ccacctgcat
    22681 aagtctagcg gcgataagct tactcctgcc caaatggttg gctactggaa agattgggta
    22741 acgaggtacc caatcatttc gattgaggat ggaatggccg aggacgactg gagcggttgg
    22801 aagctgatga ccgataccct tggtagtaag attcaactcg tgggcgacga tcttttcgtt
    22861 accaacgtgg aacgtctcca gaagggaatt gacgagggag ttgccaacag tatccttgtt
    22921 aaggttaacc agattggcac cctaaccgag acaatcaacg ctgtaaggct tgccgatact
    22981 catgccatga cggctattat tagccaccgt tcgggtgaaa ccgaagatac tgcaattgca
    23041 catttggctg ttgccctgaa tacaggctta attaaaactg gctccgcatc acgctccgat
    23101 agaattgcca agtacaacca actccttcgt attgaagaaa tgctgggtcc ttctgctatt
    23161 taccccggtc gtaactttaa gtacattaaa aagtaacggt tgctttctaa aaattaaatc
    23221 gaggggttcg gattaacatc caaccccttt ttttaaattt attggcaaaa ttttgatatg
    23281 gcctataaat cgttacacca ctttatcaat aagctcgagg agcaaaacga gctgataaga
    23341 atttcagctt ttgttgaccc tgtgctggaa atagccgaga ttaccgacag gatgtccaag
    23401 caacccaacg ggggtaaagc gctcctgttc gagaatacag ggacatcttt tccagtgctt
    23461 accaatgctt tgggttctat aaaccgaatt tgtttggcct tgggcgtttc tgggctcgac
    23521 gatgttggct ccgatattaa taaactattt gccgagttgg ccgggcccaa aacaaacatt
    23581 tgggagaagc ttggcacaat tccaacgcta ggcaagctct cggcttggct tcccgctcaa
    23641 atcagctcga ggggtgcttg ccaggaggtt gtccacgccg agcctaatat caacctgcta
    23701 cccgttatgc agtgctggcc ttttgacggt gggcgtttca tcaccctacc catggtaaat
    23761 acaaaagacc ccttcaccgg tattcgtaac gtgggaatgt accgcatgca ggttttcgac
    23821 ccaaaacata cagggatgca ttggcatcag cataaagttg gagctcgaca ttttgaagcc
    23881 tacaaaaaat taggcaagcg catgcctgtt gccgttgcgc ttggtggaga tcctgtttat
    23941 acctatgctg ccacggcccc aatgcctgat aacatggatg agtacttact tgcgggtttt
    24001 ctacgaaaaa aaaaggttaa gctggtcaag tgcctgacgg tagatttaga agtccctgag
    24061 gatgtggatt ttgtactgga gggatttgtc gatccaacgg aagaacttgc atgggagggg
    24121 ccattcggcg atcataccgg tttctactct cttgccgact ggtacccccg ttttcactta
    24181 acctgcatta cccatcgcag ggatgcggtt tatcctgcta ccctggttgg tgtgcctccc
    24241 atggaggatg cctatattgc caaggcaacc gaacgaatat ttttacagcc tctgagaatt
    24301 acaatgctac cggagctgga ggatttggct cttccggtgg aaggcactgc tcataacatt
    24361 gctttagtta agttcaggga ttactaccct ggccaggcat acaaaataat gaatgcaatc
    24421 tggggcgcag ggcagatgat gtttaataag ctactggttg ctttgccaaa agacatagac
    24481 cttacaaatg ccgaccaaat acttcactcc cttgttaact ttgttgaccc cgaaagggat
    24541 ttaatgtttg gtaaggggcc tctcgatatc cttgaccatg ctactcctac acaggcattt
    24601 ggaagcaaac tctttattga tgccaccaac caaacggttg ttatgcccca ccttcaaatt
    24661 agcatcaatg atgtaaagga gctacttgaa agggaaactt ccagggtgaa aaatttcaac
    24721 ctgaatcttc tcaacagggg aatttccatt cttatcattt ctgttgataa gcaagccgta
    24781 acaaatgtaa aatcctatat gcagcaactt ttccgttgcc aggagttggc tggattaagg
    24841 gctatggttg ccgttgacat gggtgatgct gttaccgatt tgtctatgct aacatggttt
    24901 gcttcaagta acatcgaccc gataagagat gtcttcatac tcaacctgaa cgatggttac
    24961 ccagcccgag ttggagttga tggcacccgc aaggcatacc ctcaggataa ttttaagcgc
    25021 gaatggccaa atgttgtggc catgagccgg gaggtggttg accacgtaaa caaacagtgg
    25081 gaaaaatatg gaattggcgg aatggttgaa tccccctcga aacagctttt accccttgtg
    25141 gctggtgaag ggccggtggt aaagctcaac agctgaactt ttacattgat atggcttcac
    25201 cataatcact tttttgggac tcatagctgc attcaactta ctgtttctga gttagcgctg
    25261 agcatccgct taaaatttcc tcaggagtac cgtttaatgc tgcaggatat aggctactac
    25321 ttgggccagg tggttatcat cctgataaat tcaagcctag ttttgttatg ctgaaaaata
    25381 agtatattag cgggtaccat gagatgtaaa gctgttttta aatgcttgtt gccggtgctg
    25441 ctttttttta taaaaggcgg aggtgaactt tttgctcaaa aaacagaggt tgaagtgttc
    25501 gatgctaaca atggcctgcc ccaaacctat gttagctgtt taattcagga ttcaaaggga
    25561 tttttatggc ttggaaccca tagcggcctt tgcctatatg atggctacca gttcactact
    25621 tttagcaaca accctttaga ttcattgagc ctttgcaaca acttcgttaa atcaatagct
    25681 gaggacaaaa atggcgatat ttgggttgcc acactatcgg ggttaagccg ctatgagcgg
    25741 gcaaccggaa aatttattag ctacttttcc gaaccaacca atcctgcatc ccttgctggg
    25801 gacaaaattt actacgttta cgtagacagg gagaaaaacc tttgggttca cacacccggg
    25861 acattgaacc tgtacagcta taagtccaat gacttcactc gatttaacta ttttaacgac
    25921 ctctttaccc aaccggttga aaccgatata taccccattt acgaagacct gcaaggaaga
    25981 atttgggtgg gtaccaagga tgggttgatg ctgttcgata gggaaagaat gctttttaaa
    26041 cgttacagta gccgaatatc ctacccttac tcaatatatg ggaacagagt tcaagccatt
    26101 tatgaggata gtagaggtaa tttttgggtt ggtaccgata aggggcttaa cctattcaac
    26161 tttaagctgc agcgattcta tccggttggg gcaggcatag atgaaaacga tgccgtcagg
    26221 ttcatttttg aggatagccg ctcaaccctt ttacttggaa tggaaagtgg ggttgcgagg
    26281 ttacattctt ctacagaaaa agttgagcag ctacaaaatt tttatagcaa aagcagcaaa
    26341 attttaccta ttggcatctc tgctattcta aaggataagt cgggaattac ctgggttgcc
    26401 tcacgtacag gtttgataaa aatcaacccc tttagccaac ggtttaaggg ctacagtagg
    26461 gacaacttgg gcaatccgct tttctccaat aacattattg catctgtttt tgaggattcc
    26521 tccggaaaac tctgggtagg aacatggggt agaggtctgt tcattttcaa tccgaaaact
    26581 ggagaaaacc tgagatactg taaaagttca tcaatctacc gcttacccaa tgattttgct
    26641 catacagtat ttcaaaccag aaagggtgaa attcttgtgg gaacccggaa tggtgtcttt
    26701 gtgtatgacc agagaagcag gcagtttagc aatttttttg ccagtaggca tgttagcgtt
    26761 ggtaatattt tcgtcgataa tcgcgtgtat tccattgacg aggattccgc tggaaacctt
    26821 tggtttgcta cggcaattgg tttgcattgc ttaagtggta atcgcctgca tagctactat
    26881 tcccgtaggg gtgattccat atttttacct tcgaatttaa tttttgatgt gctagctgga
    26941 aagcaaggag aagtttgggt ggctaccgca aatggtttat cccgtatatc ggacgattgt
    27001 tcaagcgtga ttaatataac ggttatgacc gattccgctg gtcaaggaca acaaccccta
    27061 ggtgaagttt actgcttgca ccagctatca aatggagcaa ttctggctgg taccagcaac
    27121 gggttggtta gcgttaactt gttgaaaggg agcaaaaacc tttatttcga aaaccttatc
    27181 atcccatcaa tatccgcaag ggcaattgag gaggatgtaa agggtaattt ttggcttagc
    27241 accagcaagg gtattacctg ctataaccca tcaacagggc aacttgccag ctatacatct
    27301 gacgacggct tgctggggaa cgagtttaac ttgttgtcta gcttccagtc gccttcggga
    27361 acaatatact ttggagggat gcagggaatt agctacttcc atcccgattc ggtaatgctt
    27421 aatagccata ttcctccatt aagttttact aaaatatcct tagcgagcgt aagcagtacc
    27481 aaaaatattc acccttttgg tatagatgaa gtaagaatcc cgcagggatt tagctcactg
    27541 actattgaat tttcggcgct ggattttaac catcccgaga aaaatttata ccggtacagg
    27601 ctacagggac ctggtagtag caagtgggtt gaacttggca acaagcattc aatcacctta
    27661 acgaacttta gagagggtgt tttcaggctt gaggttatgg gggctaacag cgattgtgtt
    27721 tggactactg aacctttatc cctccggatt attgttgttg ctcctttttg gcgatcacgt
    27781 attgcataca ctgtctatgc cttgctttta ctattcgtat tgatcgctta tggaagctat
    27841 cgaagaagaa attcactaag gataaaaagg ttgcttgcag agcgcgagga tgttatggcc
    27901 aagcttgaac ttcaaaaaga ggagctggaa aataagaaca aaaatataac cgatagcatt
    27961 cactatgcta aaaggattca ggatgccatg cttcctactg ttgacatgtt taaaagccat
    28021 attccaaatt ctttcatcct ctttaaacct aaggatattg taagtgggga tttttattgg
    28081 gtaaaggaaa ctgatagtaa atgctttgtt gccgttgttg actgcactgg gcacggagtt
    28141 cccggcgcat tcatgtcaat cattggcatt gagctgctta gaaacatcac cgaggttgag
    28201 gcagttgagg atactgctga agtgctgaat aggcttagcg ctagtgttca tgaaaccttt
    28261 tcgtcggtta gctaccctaa cgaaagtgga cttaaggtta aagatgggat ggatgttacc
    28321 ttttgcacct ttgataagca gtatgacata atgcagttta caggggcgtt tagcgatttg
    28381 tacattatcc gtgatggtaa tattatcgag gttaagggcg acagattttc tgttggtatg
    28441 gctgatgatg cagggcgcta catgtttagc agctcataca tacctttaat gcccgacgat
    28501 atgatatacc tttttaccga tggttacgtt gaccagtttg gtgggcccga ggggaaaaaa
    28561 tttaagtttc gtcgcttccg ccgcctgctg ctcaacattt ataggctacc ccttgaaact
    28621 cagcagaaaa tacttgagga aagtatcacg gaatggatgg gcggtcagga acaggttgac
    28681 gacatcctgg ttattggcat tcgtactcct actaaggcaa agtagcatgt agggttaagc
    28741 tgctagctac ttgcctgagg cggcatgttg atcatctaac ccttttaatg ccatctaaac
    28801 gattctaatt tgtttctagt tatgaagcac ccatgtagga cggctagcac aaacacgtat
    28861 gaacaaccgg gcgttacttg cgggttcgac gaagccaaaa atggatgttc catatattta
    28921 aacccgatgc ctaaagggta tagaagtcag aaatctaaaa aattggtaag gatgaaacga
    28981 ttagcaatcc tgttgctgtt ctcgatttta actcccattc agaattttgg ccaaacagtt
    29041 agccttgtgc ttagcggggg aggagccaaa ggatgtgccc acattggtgt aattaaagcg
    29101 ctggaggaaa acgggattcc tattgataat gtttcgggaa catcaatagg ggcgattgtt
    29161 ggtggcctat acgcaatggg ctattcaccc gatgaaatgg ttaatatttt taagtctaga
    29221 gatttttaca actggagcaa tggcctcatt gatgaaagct ataagtttaa catcaacgat
    29281 ttgtcaacta ccgatgccga gaacctgagc gtggggttta cctttgacag aggcgggtta
    29341 aaaactaagc ttacctccag catagtaccg ccggtagggc tggatattgc ctttgaggaa
    29401 atgtttgctc agggaacggc gatttccaga ggaaattttg acaaactttt tgttcctttt
    29461 aggtgcaacg cctccgatgt ggtaaacaag aagcaggttt actttagaga gggtaacctt
    29521 ggtaaagcaa tccggacatc catgacattc ccgctttact tcacgccagt ttatattgat
    29581 tccctactac tgtttgatgg gggaatatac aacaattttt tatggaagga agcgctgcag
    29641 gagtttcatc ccaacattat tgtggggagt aaggtggcct ctaacagcaa gctgcccagc
    29701 gatgaagacc ccttgcttca agtcgaggct atgattgttg gcattaccga ttatagcatt
    29761 cccgattcgt tgggaatagt tattgatacc cggttggaga atattgagct acttgatttc
    29821 gataaggttg atgaaattgt ttctgcaggg tacaacgaaa cacttaaggt tattcccaag
    29881 ttgagggaac aaattacaag aaatgttacc ccggaggagt tggccatgag aaggaaaaac
    29941 tttcgtcaga atcttccccc gctatttatt gacaacatcc gggttggaaa cctttcggac
    30001 aggcagaaaa cctacctgca caaggtaatt tttaacgagt acacaattgt tacctttgat
    30061 aagtttaaaa aggattacta ccgcttggta tccgatagaa cctttaagcg gttgataccc
    30121 gagttcgaat tcgactacac ttcgggactt tttgatgtgg ctttaaatcc taccctaaat
    30181 aggtctgttg atttgggcct gggccttagc ctttcttcgg atgtgggcaa cgaggggttt
    30241 gcttctgcaa gctataaatg gctatcgcga acctccaaca caatctatgg aaatatttac
    30301 tttggaaagc tgctcagcag tattcgcctc gactacatca agacattccc taccaaaata
    30361 ccagtatcgt tggttgcctg cactattgcc agcaggtatg attatcacag cagcaatcca
    30421 attccgtttt ttgaggatgt taaaccgcct tatgttctgc aggatgaaac atttgcaact
    30481 gtatcgctga aagttagcaa taccagcgca accactacaa cgctatctgt atcgggagga
    30541 gaaaaggttg accactacta ccaagctatg gattactact cttttgatgt tcctgaccaa
    30601 accacgttta aatttattaa gggttcgctt tgtttcgata agtacaccct ggattctaaa
    30661 cagtatgcca cacggggtag gcatcagcag ctagtaatat cgggattttc aggtagagag
    30721 gagcactacc ccggaacaac cgccccccag atgaccggag taagaaaaaa ccactccttt
    30781 gcaacatttt atctttacaa tgaaagctac catcgtattt ggagaaggcg attctggctg
    30841 gagctgttat ttgagtccta ctactcaacc caagggcttt ttagcaacta ctttgcatcc
    30901 atccttactt ccaaccagtt ttcgcccacc ccacactcaa acctcatttt tatagaaaat
    30961 taccgcagta ctcagtatgc tgctgtaggt gtagctccaa tttttgagtt ctggaaaaac
    31021 gtacatttta gggttggcgc ttacctttac cagccattta gaaccctaaa gcaggatgat
    31081 gtgggagttg caaattatag cgattatttt gctaagcgat ggttaattgg atcggcttcg
    31141 ttggtttatc ggacacctgt aggcccaatt gccttaacac taggctacta tccaactgcc
    31201 aacagcaatg gagacgatat tttcttcaac ctaacctttg ggtactcaat cttcaatagc
    31261 agggtgtttg ataactaaac agttacccgc tactccagaa atttcttcat tttctgaata
    31321 agcacctcta tctggaatgg cttgcttatg taatcgtcca tacctgccgc taggctttcc
    31381 tccctgtcgc ctaaaagggc gttggctgtt attgcaatga tgggcgtatg gctgttggtg
    31441 ctagcctcga tctccctaat tttgcgagtg gcaacatagc cattcataat gggcatctga
    31501 acgtccatta gaataatgtc gtattgactg gtgccaaatt tgtccaacgc ctcttttccg
    31561 ttggcggcca catcaatatt ctttaccagc tttttcaggc ttagaatcac tattttctgg
    31621 ttaatcaggt tatcctctac cagcagtacg tttgcattac taagtttacc gtcgccctta
    31681 gcctccgccg agggttgggc agtaagggta gaaatttcgt catgctgtct ttgggtagtt
    31741 ttaagaactt cgggttcagc tactttttta atttcaggta ggctgttcaa aaagtaggta
    31801 aagctaacta cagcatcgtt agaggtcagc ttgatgttga gttttccgcc gttgctctgt
    31861 accagattgt tcgttatggt caggtctagc aggtcaatga aaaattggtt gtttaatgaa
    31921 gaggaggttg aatcggcaat tacagtttgc gaatacccct cattttggtg gatgagtagg
    31981 ggcacgttgc tttttacctc aaaaaatagc tcgagggagt ttttggcatt gcccatccgg
    32041 ctggcaataa tctcgatgtt aaccttgctt ccctccttat tcttgattac attctcaata
    32101 atgttgagaa aaatttgttt cagcttaacg gggtcgccct ttagtgtttt gggaatactt
    32161 tcatcaattt taatgttgaa gttaaccgtc tgcgattgcg tggcaaatag gcgaatagtg
    32221 ttgttgatag tgtcagatag gttaaaggga atggcaaaat ctgtttcagg ctctattccc
    32281 gtgctcgatt tctccaccat gccgtttact acacctgcca agctgttagt ggatgcctga
    32341 atggtgtcaa tcagatccct gtacttgtcc tcggtgatgg tgtttgacag catgttggat
    32401 atgaccatga tgttgttcag gggtgtgcga agctggtggg aaagcttggc caaataatcc
    32461 tcgcgcagct tattctggtt cttctgctca atcagctgct tttcgagttc ctgtagcctc
    32521 tttgccttga aaaggtaaag cagcgaggca actagctgca gcagcgggta aaatatgatg
    32581 gaaagtaaac ggtagtagcc cgttgaactg acactttcac caataaattt agggccggta
    32641 atgagaatgc acgagtatag aatccagaaa ataaaggaaa taatatgtgc tctgttgggg
    32701 gtagttgttg ataaggccag catggggaac atggctaccc caatcattaa tgcagcatcg
    32761 agtttaccga caatcaaaat ggccagtagg gctatacctg aaatagctaa cgcaactaat
    32821 ccgaatgagt taacccgctt cttgctataa gccaaaacga tgatgagtaa tatgcttatt
    32881 gcagcaacgc ctgcaattat ccccgatgcc acttgatgtc taaaaagcag caatgtgcaa
    32941 aagacaacaa gatagagtag gaagggtatg gttacaatgc ttagttgaac cagtctatcg
    33001 gctgaatccc ttgcagtttt cctttcaatt tttaacgtga aaaaggttaa gattttttca
    33061 gctattttca ttgctttaag aaaattgagt aaagattgct accaaaggta gtttaaaaaa
    33121 tgtaaaagca ataatcggca cgagcagaat tttacctgta tcttttatgc ttccctcgcg
    33181 gtgctgccga agaacaaccc taatagaaaa atgtaaagct gcttattcaa cataatgagg
    33241 cctaatcttt aatgacctgg aatactcagg taaaggtagg taacgcctat aatatacaaa
    33301 cccgatggtt tgcgctttac aatccatgcc gttgaaaaca aaacgcacta ttggtgattg
    33361 cctgattggc ttcacaaaat ccccgtgcat ctggtaataa aagattgtat taacggatga
    33421 aagatttgga tggccaggga aaggcagcat cagggaagtt gtccaaacca tgccttatta
    33481 accactccaa cgatagcaat tatgccaaga atgaaaataa ttgaaccggt tatcttgttc
    33541 atccaccaga gctgcttgag ccggaactta tggcggaaaa ggtttaccag gctgctaagg
    33601 gtaaaccacc acaagcttgc ccctaaaaaa acgccaacca gtgcggtagc cgtaaaaaga
    33661 aggttttgat tctttgtaac tattcccagg gcagcaaaca gggcaataaa aagaaaaatg
    33721 gtaaatgggt tggctccggt aagcaagaac atggagataa aatcctcaac aagggtgttg
    33781 ggcttctttt tgtgtcgctt aagctgggta accggattag tgtagaatgt tcttaacccg
    33841 agtaggataa taataatccc ccccgcaagt tcaatgattt gctgcttggc ctcaataaat
    33901 ccaataataa aggtcaagct gaacccgcca attgctgcaa atatagtatc cgtggttgca
    33961 gcacctaaac ccgagaaaaa tccgcttatc cttcctttgt taattgtacg ctgaacgcat
    34021 accacaccaa caggtccgag cggaattgaa gcggcaagcc ccattatgat tccttttaga
    34081 agcaggctaa aaatcattag atgtcccttc aatattacaa ctacatacaa gttggcaaat
    34141 ataataaata tagtctaggt taggcttgag gctaaagctg attaaccaaa attatgactt
    34201 tattcattga ccaaaaattc attgggctct tttgaggctt taattattaa atggtcattt
    34261 tcactagtga agacaggata tacaaataat cttatcttta caccgaacta aaatttttat
    34321 ttgtatcaat gagaaaattc tataacccga atttttgcag ggtgtttttt agtgggattt
    34381 tgctagctct gccgtggtat gaaggtctcc ctagctactc gatttttttt ggttttgtcc
    34441 ccctgctgct ggcttacgac aagctaaatg aaaaaggtgc aggtttctgg aagttgatgc
    34501 tttatgcatt tatcgccttt tttacatgga atgcattgtc tacatggtgg atttacaaag
    34561 ctaccatgtt gggtgccatt cttgcggtgc taattaccac catgcttatg acccttgtct
    34621 ttgccggcta ccattttgtc cgtaagtatg ctggcaaagt tgccggaata tttggcttta
    34681 taacactttg gatagcctac gagcgttttt ttcatgactc tgaagttgcc tggccctggc
    34741 tatcgttggg aaatagcctt ggaaaccatc caaaggtaat tcagtggtat gagtttacag
    34801 gtatgatggg gggctcgctt tggattctgg tgatcaattt tcttatactg gggttggttc
    34861 atgccataag ggggaagcaa attcgtcgtg ctggatggtt ttctatagca gcattactaa
    34921 ccgttattct tccactgtgg atatccattt cgatgtataa caggtatcat gaggttcaga
    34981 accctgtccg ggtggttatt gttcagccca gcatcgatcc ctacaacgaa aagtttagcg
    35041 gcttaaccaa cgagcagcaa ctcgatatta tgttcagcct ggcaaaacag gctgctgaca
    35101 gcactaccga ttacgtggtg tgtcccgaaa cggccattga tgataggatt tgggagcatg
    35161 agctacatgt taactccagc attcttcgaa taaagcaatt tgtcgatagc cttccaaacg
    35221 taaaatgggt tacaggggca ataacgctta aactattcag ctcttctgaa aacctgcctc
    35281 caactgcacg tgaattacca tacagggagg gctattttta cgatcgctac aattcagcca
    35341 ttcaggtcga tactgctttt caactcccca tttaccacaa atcgaagctg gttgtgggcg
    35401 tggagatgat gccttacccc aagcagctca agtttctgga gaagcttgct attgacctgg
    35461 gtggaatatc tggcagcttg ggtactcaac ccgacagggg tgttttatgg tcgcccgaca
    35521 ggcgttttgg tgttggcccc attatctgct acgagtccga ttttggcgag ttcgtgtcgg
    35581 gctacgtaaa aaatggagca aacctcctgt tcgttatcac caacgatggt tggtggggtg
    35641 ataccccagg gtacaagcag cacctttctt tttccaggat tagagctatc gagacgcgcc
    35701 gttcagttgc ccgttcggcc aacacaggga tttctgccat tatcaaccaa cggggtgatt
    35761 tagtccgtac gctgggatgg tgggagcgtg gcactatttc aggaacaatc aacgcaaata
    35821 gtaagcaaac atggtatgta agagaaggcg actacatcgg taggctgtct gcattcatgg
    35881 cagtacttac ccttgctgca gcccttgcag cttacctaat gaagaataat ggcaggagtg
    35941 ttaaacggtc ataatcctct aattctattt caagcaatag tatttgtatt gctgcacttc
    36001 tagtattgtt cgctttatgc agtttaataa cttaatgctt gcacagcctg cattttttgt
    36061 ttctaaatac acaccacttg gcttcctgtt tggtgcggat gcgaaattca gaatttatct
    36121 cgggttttaa gatctctatt ttggcaatga ggctagtgct attttacggt tgtagatttt
    36181 ttaaattcct ctaccccttt gtctaggaat gcaacaaatt catcaacgtt agtattgtat
    36241 cccataggtt cggtaagcat ctgctcgttg gttcccagca atacgtagta tggctgtccg
    36301 ttaacgttga accgtgttgc ctgaaaatcg gaattctttt ttccaatggt ttttttcacc
    36361 ttgccatcac gggttgaggt aacccattca ctttcgggaa gttcggttct atcatcaaca
    36421 tagagggcaa caataatgta gctgctgttt aagcgctcca gcacccgagg gtcgctccac
    36481 accctggctt ccatatcctt acagttagca caggtgtggc caacaaaatc aacaaaaatt
    36541 ggcttgccca acttcctggc gcactccagc gcctgattgt aatcgaaata cccctttaag
    36601 ttgtagggaa gcttaaaaat atctgcatac tttggttgat cgcaaagcga caagttggtg
    36661 ttgtcgttta cagtgggcat cacagcctgg ctattctggt taagagagaa tttctggctg
    36721 ctttgtggag gtataagacc ggaaattgct tttagaggtg caccaaacat gcccgggata
    36781 aggtaaacta caaaggtaaa gctggcaata gccaaaaata accttaacac gccaacatgt
    36841 tgaaggtcgg aatcgaactt gaattttatt tttcctagta ggtaaagtcc aagcagggtg
    36901 aatatggcta tccatattgc caggtatatg tcacgactta aaaagttaag gttgtacgat
    36961 tggtcgatgg tgagtagaaa ctttaaggaa aaagccagca caatgaaacc caacaccacc
    37021 ttcacggagt tcatccatcc acccgagcgg ggtagcgaat ttagccacga tgggaaaatg
    37081 gcaaatagtg taaagggaat tgagaaggcc aacgaaaatc caaacattcc aacaataggt
    37141 ttagcaccta gcccagaggc tgcctcaaca agaagcgctc ctacaattgg gccaacacat
    37201 gacaggctga ctataaccgt tgtgagtgcc atgaaaaacg agcccgcaaa tccaccctta
    37261 tcggccttac tatcggtttt gttggctacg cttccgggta gaacaatttc aaatagcccg
    37321 aagaaggatg ccgcaaatgc aatgaaaagt agaaagaata tcaggttggt aatccagtga
    37381 gtggtgagcg tggtggtgaa gtcgggtccc aggttgaaca gcgataccag taaacccaac
    37441 gaggtgtata tcacaatgat ggacacgccg aaaactatgg cgtttagtat agcattcatt
    37501 ctgttgccct gctttcccat aaagaaggaa acagtcatgg gtatcatcgg gaacacgcag
    37561 ggtgttagca atccggctag gcctgcagca aacgatacaa agaaaaatac ccatagggag
    37621 ctgcttgatt tttttgcact ttcatcattt gttggtgcaa gggttgcagt tgttgtcttt
    37681 tcgggttgaa cctcttcacc ggcaagtact acagaaccat tctttcctag cttgtggctt
    37741 aaatcctttt caaggtatat gcacttgtcg gcgtagcaag cctgatattc aaccgtaagg
    37801 ttaatgcttg cctcatcacc tgcaaggtca actatttgac caaactgcgc ctccccctcg
    37861 aagaacgaaa ggtcaaggtt gaaaatgtcg tcatgctccg ttttgggttt ggttagctct
    37921 tttacccctt caaccagctt gtagtccttg ctgtcggcaa agttaaagct ggtgggcaga
    37981 ggcccgccct cgggcagctt tgttgagtac aaatgccagc cttgctctat cgtggctgtt
    38041 ataataagtt cgtatttttt ggcatcaatt ttttttgacg agatgctcca ctgaaccggg
    38101 tcttctacct gtccaaatgc actaatgctg cccgcggtga gcaacaccat ggttattccc
    38161 ctctggataa tgtttcgttt catttgccac gtttttggtt acatgttgaa ttgtgagaaa
    38221 aaatacttaa gctaccttgt cgaggttagt gaattttatc tctatcttgc tcatcactta
    38281 ccggcttttc tacaaaaatc cataattcgt ttgtgggtta cgggttaaca acttcgggcc
    38341 atagaaagtt gtaatcattt tattcgctgg tgcaccagca aaattaaatt ttccctcgta
    38401 gtttttgttg cggcaaatat atagattttg aatcggctta ctttttgatt tttgtcattt
    38461 aagagctttt gttaagaaaa aatcgccaca ttttctactt ttgtagtacc ccattgagcc
    38521 aaaaaaagtc aaccagggta gctttcagca tgaagttgtt tacaacaaaa cagattcgcg
    38581 aactcgatgc ctacaccatt aagcatgagc caatatcctc atatgaattg atggagcgtg
    38641 ctgcaaatgc actttacagc tggtttgcac agaatctgtc aaccctgcaa ccaattgtcg
    38701 tgttttgtgg cccaggcaac aatggcggcg acgggttagc cctagccagg ttactttaca
    38761 cttcgggcta cagcgtcgaa gtttattacc taacagcttc tgcacactct tccgattttc
    38821 agctcaatct ggagaggctg aaagcgatca attttttacc ggttgaaatt ttttccaact
    38881 ccgatttccc tcatattggt gagaatcaag ttgttgttga cgcaattttt ggttctggtt
    38941 taagcaaacc gattgaagga ttggcagccc aacttgttag tcatatcaac acctccggat
    39001 caagagttgt tgccgttgac attccatctg gtctgtttgg tgaggagaat ccgttcccca
    39061 attctaatcc cgttgtaagg gcagctgatt gccttaccct tcagtttcca aaactttcct
    39121 ttttttttgc cgagaactct tctttcgtgg gcagatggca tgttcttccc atcggattgc
    39181 atcccgaagc catagctggc gcatcaacct cctggcacta tacaaatatg gagaccatca
    39241 ggggaatggt caaaccccgg gaacgttttg cacataaggg tatatttggg cattgccttg
    39301 tggttgccgg aagtcatgga atgataggtg ctgccgttat ggctgctatg tcggctgcca
    39361 gggcaggtgc cggactggtt acgcttcatg tccccgcctg tggatacgaa attgcacaga
    39421 cacttgcccc aaacattatg gtggtatgcg atagcaatga gcaccactta tcagcagttg
    39481 atgattttca aaggtatgat gcggtttgta ttggcccagg actagggcaa caccccgaaa
    39541 ctgccgatgc tctcggtaaa atcctagcag agatcaacgt tccacttatt cttgatgccg
    39601 atgccctgaa cctggttgca gctaacccga agctactcgg ggcaatccct gctggcacca
    39661 tcattacacc gcatcctggt gagttcgaca ggctgtttgg cgtttccact tgtggctgga
    39721 aacgtttgga gcgtgccagg gaggttgctc agcgctatca gcacgtaatt attctaaaag
    39781 gtgcttactc ccaggtagtt ttgcccgatg ggcgtgtctt ttttaattct accggaaatc
    39841 cgggcatggc tacaggcgga agtggtgatg ttctctcggg tatcgttgta ggttttttag
    39901 cccaaggttt ttctatagcc gatgcggcgc tacttgctac ctttgtacat ggtttggccg
    39961 gcgatatcgc tgccagcgac ctaggtgaga atgcccttgt tgcaactgat atgattagct
    40021 acatttcaaa tgcactactt gaaattgaaa tctcgaatct gtaaatttag ccaaactatg
    40081 atcatgcgct tttcatcaat gatggttggt cttttcttag gcctgactat gctctcgtgc
    40141 agcaacaaaa tggcaaccgt ttcgcctgtt aggtctgctc caccagtaaa ggattacagc
    40201 ttcatataca ccttaccccg aactgttatt ttgattaagg ttgatgcctg tcgtagcgtg
    40261 ttctatcctg gtccctatgc tgcgtatgcc tggaaatact taggaatttc cgatgtgcca
    40321 actctaaaaa gcgaacagtg gaccattacc gatatcacta ttaaaggtat aagtgagtcc
    40381 gatccatcca tgctttttgc cgtattcacc gatggtcatg tgcctgatgc tctggttcag
    40441 atgactgagg ctggcttggt aattcccgaa aatagggctg ttattgtgtc catgatgagt
    40501 aaagatttaa ggagaataga aaatcaggct atacctttct ccgacctatc ggttgaaccc
    40561 tttattgggg aggagaaaaa tattttatac accaaagtgc agcgcgatag tagctttgta
    40621 agggttccta ttcaaaaaag catgattgtt gagaagaatg ctgatgaaaa ggctggcgag
    40681 gcagccgacc tcattttcaa cctacgcaag cgaaggctgg agttccttac tactgatgtt
    40741 gatcagccct tggagggcaa tgctgttcat gtaattctcg atgaaattta taggctcgaa
    40801 aacgaatacc tttcgttgtt cttaggtaaa acagctgttg atacgatttc caaaacctat
    40861 ctttttaccc cctcaggaga aaataatgaa aaggctatcg cctttcgatt ttcccctaca
    40921 aaagggctgc ttgcccaaaa tgacttgtcg ggtagtccca tgcttgttga ggttgaaccc
    40981 gaaccggttc ccgattcctc cgaacctctt ttggcaacca tcggtcgtct tgccgaaaag
    41041 caaaagctgg cacaaatttt ttaccgtatt ccggttgtct caaccgtaaa aatttccgac
    41101 ggtaagtcgg aaattgcttc ccgccgcata cctatttacc agctaggacc tttggttaaa
    41161 attcctgcag agttttatta atccaggtaa gctaaactta ttcaccgctc gttagcttgc
    41221 ttacaaactt tgaggcaaac tgttcccatg gttgtgtctt gtaggcatcc gacatgatgt
    41281 aaaccataat aggctcaatt tgcgatgggg taaggtatcc cgaaaggaag gttattaagt
    41341 ttccattccc atccataaaa gcaattgaag ggtaggcaag cttgccctgc agtagggcga
    41401 tagcaaaatc gtgggttgac cgttgcccct ctccttggtt tttatatact tgccccttgt
    41461 aggtaatttc attctttccc tcagcattga acttaacggc ataaaaatcc ttggttaggt
    41521 agcttgcaat cagcgggttg ttaaaggtat tgctgtccat tttcttacac cagccgcacc
    41581 agtcagtgta aacatcgata aatattttac gcggctcgct ggtgttcagc tttaaggctt
    41641 cttcaagtgt gtaccactta accttgctac tctcctgtgc tatggtaact ccagctgtta
    41701 atgttagctg ggctaacagt aatgcaaatc ttctcataca tttcaaatgt ttaccacaca
    41761 aaggtaagta aattgatgac tatggaattt gccctaaggc taatttttcg taaaatcggc
    41821 acatcaaaag cccaaaatat aggtgttcct cccgtttttt taacgatgca caaaagttaa
    41881 ggggtaaagt accttgtaac cagaaaccta aaaattcccc tgattaaatt ttttacctac
    41941 ctcaaatcat aaggctaaac aaaaaaatgc attcttccgt ttggctttat ttccgatttt
    42001 ttcccaattc atttgcacct ggagcataat ggtattttcg ggatattaaa attcttgcta
    42061 tctttaaaaa ttcaaaaaac atgtttacct aacgtatatt gctatgaata aggatatttt
    42121 taaactcgag cctcaaaggc tatggaagca cttttacagc ttgacacaaa tcccccgccc
    42181 atccaaaaag gaaaaggctg ctgtggattt tatgattaat ttcgggagaa acctcggact
    42241 cgaaacaatc accgatgagg ttggaaatgt tatcatacgc aagcctgcca ctccgggtat
    42301 ggagaatcgg aagactgtga ttttgcaagg gcacctcgat atggttcccc agaagaacag
    42361 cgataaaaag cacgattttg agaaagaccc aattgaggct tatgttgatg gagaatgggt
    42421 taccgcaaac ggtacaactc ttggcgccga taatggcatt ggtgttgctg cagcaatggc
    42481 ggtgcttgaa gcaaaagaca tagctcatgg ccctgttgag gctcttttca ctattgatga
    42541 ggaaactggc atgaccggtg cctttggcat taaacccgga cttcttaagg gtgatattct
    42601 tttgaattta gattcagaag atgagggaga actctacgtt ggttgtgccg gtggtcttga
    42661 tgcaaatatc cggtttagct ttactcctca gcctgtacct tccgatatgg ttgcctacaa
    42721 gcttgccgtt cttggactta agggtggcca ttcagggctt gaaatcaacc ttggtagggg
    42781 taattccata aagctactat tccgattcct ttaccgtgcc tgcaaaacac taggcgttcg
    42841 cattggcact attgatggtg gaagccttag aaacgctata cctcgtgaat cattcgctgt
    42901 tgttacagtt ccaagtaaga atgcaaccaa ttttgaaaag ttggttcgcg agtacgaaca
    42961 aatctacaaa aacgaatttt ccctaacgga gcccaacctc tcatttacct gtaaaaccga
    43021 ttctttaccc aaattcttag ttgatgaaaa ctcccaatca cgggctattc gcgcattttt
    43081 cagctgccct aatggtgtgg ttcgcatgag cgatgctgtt cccggacttg ttgaaacttc
    43141 gaacaacctg gctagagttt ataccgaagg aaaccagatt gttggacaat gcttaatgcg
    43201 tagctctgtt gactcggcaa aggaggcatt agctgataaa atggctgcaa tctttgagtt
    43261 agccgatgca gctattttat tcgaaggcgg ctaccccggg tggaaaccta atccaaattc
    43321 cccaatcctt actgtaatgc agcaagttta taagaataaa ttcggaacca ctcctgaggt
    43381 aaaggcaatc catgctggtc tggaatgcgg cttgctaggt ggggtttatc ctaacctgga
    43441 catgatatcg tttggaccaa caattcgcca cccccattca cccgacgaaa aggtgaatat
    43501 cgctacagtt ggaaagttct gggaatttct agttgaagtt cttaaaaatg ttcctttaaa
    43561 g
//
LOCUS       DAOSHM010000017        42412 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_698_length_42412_cov_20.180395, whole genome shotgun sequence.
ACCESSION   DAOSHM010000017 DAOSHM010000000
VERSION     DAOSHM010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 42412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 42412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42412
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_698_length_42412_cov_20.180395"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(150..1967)
                     /locus_tag="PK839_04365"
     CDS             complement(150..1967)
                     /locus_tag="PK839_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG72005.1"
                     /translation="MKKFFSLLKFIIPYKWHVVANVVGNILGAIFSLFSLAMLIPFLQ
                     LLFGTVPLVEVKPEFTMTIKSLNDTLNYMLSEMIRNHDRSTAMLFVIGLVLTASFLKN
                     FFTYMSLYYLAPIRTGVLRDIRNTLYRKVVDLPLGYFTEEKKGDVISKMTNDVNEIEV
                     SIIRSLEMIFRDPIIIIIHLIGLIYISPQLTLFVLLLLPLTGVVIGRVGKTLKKTSFK
                     GQRKMGAILTLIEETLSGLRVVKAFNAEKKVISRFESVNNFYTLLMKKMWRRRDLASP
                     LSEFLGTIVVVLVLWFGGNLIFKGKGTLTPEALIAYIGIFYMIINPAKSFSNAYYNVL
                     KGMASADRIDTILSAENPIKIKPEAKVLTEFSSAIEFRNVNFRYQSEPVLHNINLVVE
                     KGKTVALVGQSGSGKSTLVDLLPRFWDVTEGEVLVDGVNVKDCNLESLRNLMGNVNQD
                     PILFNDTFYNNIAFGMPSATEEDVIAAAKIANAHDFIMATPNGYYTNIGDRGSKLSGG
                     QRQRISIARAILKNPPIMILDEATSALDTESEKLVQDAIENLMKHRTSLVIAHRLSTI
                     RNADMICVLHEGEIVERGRHEELLRLNGYYAKLHNMQLK"
     gene            complement(2032..3378)
                     /gene="purB"
                     /locus_tag="PK839_04370"
     CDS             complement(2032..3378)
                     /gene="purB"
                     /locus_tag="PK839_04370"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HOG72006.1"
                     /translation="MSLNALHAISPIDGRYYSKTKELQNYFSEFALIKYRVWVEVEYF
                     IALCNLPLPQLKKFRTGNFDKLRGIYEKFAEGDAARIKNIESTTNHDVKAVEYFLREK
                     FDELGFSDYKEFIHFGLTSQDINNTAIPASIRDFMNSCYYPTLSKLLEKLHDLSNEWA
                     EIPMLAHTHGQPASPTRLGKEIRVFAERIEVQLLQLRSIPYSAKFGGATGNLNAHLVA
                     YPEVDWVAFANGFVENTLGLKRSQTTTQIEHYDNLAALFDCLRRINTILIDLCRDFWA
                     YISMEYFKQRIVEGEVGSSAMPHKVNPIDFENAEGNLGLANAILDHMATKLPISRLQR
                     DLSDSTVLRNVGVPFAHIQIAIQSIVKGLNKLIINNEVIKNDLETNWMVVAEAIQTIL
                     RREGYPNPYEALKKLTRNNKNVTQKDFLTFIDELDIDSRVKTELKSITPFNYLGVK"
     gene            3490..4248
                     /locus_tag="PK839_04375"
     CDS             3490..4248
                     /locus_tag="PK839_04375"
                     /inference="COORDINATES: protein motif:HMM:NF014658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis protein"
                     /protein_id="HOG72007.1"
                     /translation="MEKLVKISAISYLNTAPFAYGLRHSDIFSKIQLLSDYPSECARK
                     LQYNEVDISLLPVGALPSLSHYEVITDFCIGAIADVRTVVLLSNSPLHRIRKIYLDYQ
                     SRTSVQLVRILSRYYWNINPEWVPLSPEQDYSTIPKDYGIVLIGDRVFEVESRYQFVY
                     DLSGEWGKFTGLPFVFAVWAAASQPTDDFKHQLNHALQLGVNSIPQSVDEFALKNIER
                     DEAIRYLNQNISYPLDAEKRKAISLFLSYMSQNQ"
     gene            4330..5760
                     /locus_tag="PK839_04380"
     CDS             4330..5760
                     /locus_tag="PK839_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HOG72008.1"
                     /translation="MKRLKDVASRLACGIVAVFLLTIPFHLVYAGEPATNSPTVVYKI
                     DIQENIMPAAWRHVKRGFEEAARINADIILIHLNTYGGMVDMADSIRTKILNSKIPVW
                     VFIDNQAASAGALISIACDSIYMRKGGSIGAATVVDQSGNVVPDKFQSFMRGMMRATA
                     EAKGKDTIVTKGDTLLKWRRDPRIAEAMVDPSIFIEGIIDSGKVLTFTADEAILHGYC
                     EGKAETVDEVLAKAGVESYRLEEFTPTFIDRIIGFLINPVVSGILIMLIIGGIYFELQ
                     SPGIGFPLVVAVLAAILYFAPLYLEGLAQHWELALFVVGLILLFVEIFAIPGFGVTGI
                     SGIILMVVSLTLAMIDNNLFRGVGSFNVLILLKPLAIVLVAGFLGFLGGLYLSKKFLT
                     SSMFSNLALNSSLSKEDGFVGVDKQIKTKIGLEGIAATMLRPSGKVKIGDDWFDAISE
                     YGYINKGERVRVTRDEAGQLYVVKAE"
     gene            5774..6217
                     /locus_tag="PK839_04385"
     CDS             5774..6217
                     /locus_tag="PK839_04385"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HOG72009.1"
                     /translation="MENFSVRDFLPSDFEALSLVWSLTGLGNPARGDNLEIIMQSIAM
                     GGKLLVATSSQGLVVGTSWMTFDGRRIHLHHFGVHPSYQRRGIGKMLAAESLRFVKQK
                     GYQVKLEVHQGNLAAINLYKQFGFQRLGDYDIYIIRDLTVIDSVL"
     gene            complement(6251..7675)
                     /locus_tag="PK839_04390"
     CDS             complement(6251..7675)
                     /locus_tag="PK839_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOG72010.1"
                     /translation="MLKIQIQEDIKSYLGYTPTSGQAAAIDILSDFTLNPDSGKCLLI
                     KGYAGTGKTSLIAGYVKTLQKLKVPFELLAPTGRAAKVLTDYSGIKALTIHKCIYRQK
                     SISEGIGKFVLNLNKLTNAIFIVDEASMISNSSFEDSAFGSGKLLSDMVEFVKQGKNC
                     RLIVVGDTAQLPPVGLDISPALDARELAMFNLDVQEIFLNEVVRQDLGSGILFNATRI
                     RQILDSTAISTPKFDVSTRFKDIERIDGGSIMEYLTEEYSRSNRNSNVVICYSNKQAN
                     RYNIAIRSRILFNEEELSVGDYLMVARNNYYWVADNPNIAFIANGEIVEVKRIGKHYN
                     LYGFRFADVTLKLTDEDNQEVDARIILDSLTIDGPSLGSEQMKNLYAAVAQDYQHIKT
                     YRTRLQKIREDPFFNAIQVKFAYAVTCHKAQGGQWPNVFIDQGFFRESMINREYLRWL
                     YTAVTRATSKVYMVNFNDIFFGNE"
     gene            7903..8739
                     /locus_tag="PK839_04395"
     CDS             7903..8739
                     /locus_tag="PK839_04395"
                     /inference="COORDINATES: protein motif:HMM:NF024271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3822 family protein"
                     /protein_id="HOG72011.1"
                     /translation="MLKPLKLIDESFDRDRTGSYDLSVTISINGFSFAVLDTLRNTFI
                     VLVSTPKSEQQDFEELFNQAMVDYPWLAGSFKHVYLSFFSPYFTPVPGKFFSEQKARQ
                     LFELVHPLPDGFELHFTPNCNHTDATLLFALPNSVANWWLKLHPKASFLHPIAPLLSS
                     PAAGSNVLLSVDFNENDFLVALHDGMKFAGANAYTGRHPNDVIYYLLAFCKELGYDSR
                     NVKVSITGHSPMLAELNMLDGYFDRVSSIAQYPKVMLFSYQLISHRSEYFSLFNLGAS
                     CE"
     gene            8730..9269
                     /locus_tag="PK839_04400"
     CDS             8730..9269
                     /locus_tag="PK839_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018463192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HOG72012.1"
                     /translation="MRIVGGSLRGRRFDPPKNFGARPTTDFAKEGLFDVLSNYFDFEE
                     LTVLDLFSGTGSISLEFASRGARHIDAVEKIYAHQKFIKDSALRLGVTSISVIRQDVF
                     SFISRSTCRYGLIFADPPYDMEGINTLPELILSKDLLDEDGWLVVEHSDRYSFSGLKQ
                     LIDHRRYGSVNFSFFRKNI"
     gene            9330..9404
                     /locus_tag="PK839_04405"
     tRNA            9330..9404
                     /locus_tag="PK839_04405"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9365..9367,aa:Asp,seq:gtc)
     gene            complement(9489..10328)
                     /locus_tag="PK839_04410"
     CDS             complement(9489..10328)
                     /locus_tag="PK839_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008860944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOG72013.1"
                     /translation="MAYNLLKGKKGLIFGALNEKSIAWKVAERAHEEGAEIVLSNTAV
                     AMRMGSIDELAAKCNAIIIPADATSIPDLENLTDKTMEHFGGKFDFILHSIGMSPNVR
                     KGRTYDDLDYDYLQKTLDISAISFHKIIQTCWKKDAITHGGSIVALSYIAAQRTLYGY
                     NDMADAKALLESIARSFGYIYGRERGVRINTVSQSPTETTAGSGVMGFDALLDFSNRM
                     SPIGNANADDCADFCITLFSDLTRKITMQNIYNDGGFSSMGMSNKAMAIYNRNLEECK
                     DCN"
     gene            complement(10389..12059)
                     /gene="recN"
                     /locus_tag="PK839_04415"
     CDS             complement(10389..12059)
                     /gene="recN"
                     /locus_tag="PK839_04415"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HOG72014.1"
                     /translation="MLRKLYIQNYAIIDELTIEFQPGLNIITGETGAGKSILLGALML
                     ILGQRVDASILKDQSKSCIVEGEFDISSYDLDDFFRVNDLDYNPTTIVRRQIGESGKS
                     RAFINEIPVNLTLLKELGDRLIDIHSQHQNLLLGTSRFQLNVVDTYAIPKNLQSAYLE
                     TYLNYRQLEAELKKLEDEAANFGRDFDYLQHQLEELRAANLKEGELEELEIEQAKLTH
                     ATEIKSALEFTINAASNDETSAIALLKEASASLKKISPYYPAADELEHRLESCRIELR
                     DIVDEAERRSSQVNVDDELLASVSQRIDLLYTLLQKHRKGTMTELIAYRNSIEERVNH
                     ISGMEFNLETRRKDLERRGHELRKKADELSILRRKAAAPIEQSITELLIQLGIKHAIF
                     KVDIQPVNEFQPWGTDRVTFYFSANSQIPPMELSKIASGGELSRVMLSLKSLLVKASG
                     LPTIIFDEIDTGVSGEIADKMGSIIHTMAKNMQVINITHLPQIAAKGKTHFLVYKENQ
                     ELRSTTGIKLLTPEERVVEIAKMLSGEKMSDAAILNARELLKSNGSLN"
     gene            complement(12078..13001)
                     /locus_tag="PK839_04420"
     CDS             complement(12078..13001)
                     /locus_tag="PK839_04420"
                     /inference="COORDINATES: protein motif:HMM:NF027445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4835 family protein"
                     /protein_id="HOG72015.1"
                     /translation="MHKSFAKLMLTLLLIAPLVLDGQELRCNVQINSQKIQGTNRTIF
                     QTLQTAIYEFMNNTAWTNHVYGFDERIECSIMLNITEQLGSDEFKGTLQVQSRRPVFN
                     SSYNTTMLNFQDNNLHFRYREFDKIEFSENSHLSNLSSILAFYAYIILGIDYDSFSML
                     GGTDYLLKAEKVVNNAQNAPERGWKAYEGNRKNRYWMIENLLNSKYRPLREVLYRYHR
                     LGLDRMSDKAVEGRAEIAECMATLQKVYREKPDPYLFALQLFFDAKSDELVNIFSESF
                     PSEKARVVNILTEIDNANAGKYQKILEQKNS"
     gene            complement(13023..14234)
                     /gene="coaBC"
                     /locus_tag="PK839_04425"
     CDS             complement(13023..14234)
                     /gene="coaBC"
                     /locus_tag="PK839_04425"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HOG72016.1"
                     /translation="MRLAGKHIILGVTGSIAAYKAAILTRLLIKEGAEVKVIMTPHAK
                     EFITPLTLATLSKNPILVDFFNPENGGWNSHVDLGLWADLFLIAPASANTMAKMASGV
                     ADNLLLTTYLSARCPVMLAPAMDMDMYSHPATQKNIQILKSYGNIFVEAATGELASGL
                     SGKGRMEEPENILQHVVNLFLAANRFNGYKVLITSGPTYEPIDPVRFIGNYSSGKMGT
                     AIATAIAQQGAQVTFITGPAAVEPQHPSVKIVRVKTASEMLEQAKNEFDSCQVAILAA
                     AVADFAPANTSTDKIKRGKENLDLQLEPTTDIAETLGKMKRQNQIIVGFALETHNEVE
                     NAIDKLTRKNLDIIVLNSLNDAGAGFGHNTNKITLIDRNGKQKSFELKPKSEVAQDIA
                     NAIIDLLQQAK"
     gene            complement(14234..14569)
                     /locus_tag="PK839_04430"
     CDS             complement(14234..14569)
                     /locus_tag="PK839_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004349573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HOG72017.1"
                     /translation="MDLKKMNVPASTITRNLDELDKETGNIYQTVRIIAKRANKLSVD
                     IKQELNHKLEEFAYYTDNLEEVFENREQIEISKYYEKLPKPTLIAIQEFMDGKLYYKM
                     VDNTEVTNQ"
     gene            complement(14587..15399)
                     /gene="bamD"
                     /locus_tag="PK839_04435"
     CDS             complement(14587..15399)
                     /gene="bamD"
                     /locus_tag="PK839_04435"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HOG72018.1"
                     /translation="MTKHRDTKGILFLFTILATIFMVSCSGYEKLLKSKDYEYKYKKA
                     LEYYEKKEHYRYTTLLEQLSPIYKGTFRSDTIEYYIAQGYYYQGDYLLAGHSYDKFRQ
                     TFQRSAFTEDAEFMYAYCFYKSSPRPELDQASTQEAIEAFVEFLTRFPNTNRRTEVNG
                     LLTELREKLVEKSYLSSKLYYKIGDYKAAIVAIQNSLKDFPNSKYREELSYLLVKSSY
                     LLAENSIPNKQRERYQNTIDQYYTFVGEFPESPYLPEAKKMYESSMKVLQIN"
     gene            15973..17211
                     /locus_tag="PK839_04440"
     CDS             15973..17211
                     /locus_tag="PK839_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOG72019.1"
                     /translation="MDIFDKIDNMGPIGQWQSQSHGYFSFPKLEGEIEPHMIFRGKRV
                     LNWSLNNYLGLANLPEVRKADAEAAAQYGLAYPMGARMMSGQTRKHEELEEELARFVG
                     KPSAYVLNYGYQGMVSIIDTLLGRNDVVIYDSESHACIIDGLRIFPGKRFVYQHNDME
                     SCRKQLEHATAVVEKTGGGILLVTEGVFGMAGDLGNLKAITDLKKEFNFRILVDDAHG
                     FGTMGATGAGTSEHFGVQDQVDIYFATFAKSMAGIGAFVASQEHVIRFLAYNMRSQIF
                     AKSLPMAMVLGIQKRLEILKSHPELRENLWNIAGELQKGLREIGFDLGRTQSPVTPVY
                     MFGGIPEAANMIFDLRENFGVFCSMVVYPVVPKGQIMLRLIPTAAHTLNDVKETVEAF
                     RVVRDRLAAGKYNKEKIAQF"
     gene            complement(17428..17658)
                     /locus_tag="PK839_04445"
     CDS             complement(17428..17658)
                     /locus_tag="PK839_04445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72020.1"
                     /translation="MVEETLWDGFDHIISELHRACVTNHMDLPPTPSAKGACYNINLP
                     ADLNNLPAKESLGSVLSRQKQAKIKESLSGSE"
     gene            complement(18100..18723)
                     /locus_tag="PK839_04450"
     CDS             complement(18100..18723)
                     /locus_tag="PK839_04450"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005633198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOG72021.1"
                     /translation="MPECLYIKMYPQNLNPRDIERAVEVLKRGGVIIYPTDTVYGIGC
                     DITNTKAVERIIKIKGMKPKEANFSFICHDLSHIADFARVDNSTFRLMKKNLPGPFTF
                     ILPGLNRVPDYFLSRRKTVGIRIPNHPIPVEIVRQLGNPILTTSLKDSDEVIEYTTDP
                     ELIFENYQNLVDLVIDGGAGNNIPSTVVDCTGDEPAIIREGLGELVF"
     gene            complement(18723..19379)
                     /gene="fsa"
                     /locus_tag="PK839_04455"
     CDS             complement(18723..19379)
                     /gene="fsa"
                     /locus_tag="PK839_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010538888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HOG72022.1"
                     /translation="MKFFIDTANLDQIREANDLGVLDGVTTNPSLMAKENIRGSENIK
                     KHYVDICHIVKGDVSAEVIATDYEGMIREGKELAALHPQIVVKVPCIREGIKAIKHFS
                     DSGIRTNCTLVFSAGQALLAAKAGATYVSPFIGRLDDTSTDGVALIRQIVEIYRHYGY
                     QTKVLAASIRHTMHIIQCLEAGADVATCPLNAILGLLNHPLTDIGLQKFLDDFKKVNG
                     "
     gene            19494..20612
                     /locus_tag="PK839_04460"
     CDS             19494..20612
                     /locus_tag="PK839_04460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72023.1"
                     /translation="MMKGWFILTILLLLTFPKVFGQQVADTLRFNNQELSANKPNDIS
                     VSTQIFLGYRYFIGNSGSTNEFTVKRGYINFIKSLNKNISGRITPDITLDREGDGQGD
                     MEMRLKYCYMKLEEPGDFWIFTNPAVFIGEVFTPWIEFEEKINPYRIQGNHFLEKSDI
                     LISADFGVMAAALLGGTLNEGYQTRVGSDYPGRYGSLAIGVYNGGGYHNIEENNNKTL
                     QYRLTLRPLPDMIPGLQLSYTGATGKGNTTLSPDWNTNVGVLSYQGQPVTLCGQVFSS
                     VGNYSGSLADTLGVSYKVWGWSAFVEFKLLRDRLSIFGRYDYVKGRNLSEFGNITQSV
                     VGVAYSLFRRNKLVVDYSRNESSGMVEEVIEFMVELAF"
     gene            complement(20678..22348)
                     /locus_tag="PK839_04465"
     CDS             complement(20678..22348)
                     /locus_tag="PK839_04465"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HOG72024.1"
                     /translation="MKSDIEIAHEVKMKPVTEIAGKLGIDADDLVLYGKYKAKVPLKY
                     IDKNKYQKNKLVLVSAISPTPAGEGKTTVSIGLAQALNRLGKQTTVVLREPSLGPVFG
                     VKGGATGGGYSQVLPMEDINLHFTGDFAAIERAHNLLAALIDNNIQSKKRSLGIDPRT
                     VRWKRIMDMNDRSLRHIIVGLGGTSHGVPRETGFDITAASEVMAILCLAENIDDLKKR
                     LGNIFIGFTYDKKPIYARDLNAQGAMAALLRDAIMPNLVQTIEGTPAIIHGGPFANIA
                     QGTNSVLATKLGLSLSDFVVTEAGFGFDLGAEKFIDIKCEYAGLNPNTVVLVATVRAL
                     KYHGNKPLKEVSQPDLEALKRGIGNLQKHVENMKQFGICPVVAINRFTSDTDEEVEYI
                     KQVCRDMSVQVAAVDVWGKGGEGAIELAEIVNKIASSCSAQHKTLYDWSWTPELKIET
                     LAKRIYGAEAVDYTPQAKADLEKVYSLGLDKLPICVAKTQKSLSDNPDLLGRPKDFVV
                     TVREIEVASGAGFIIPITGQIMRMPGLPDEPAAERMDVDNNGVITGLF"
     gene            complement(22637..23647)
                     /locus_tag="PK839_04470"
     CDS             complement(22637..23647)
                     /locus_tag="PK839_04470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72025.1"
                     /translation="MNRVILFSLTALVLLISCKPNRTERIDPTNVPASIQLERFEQDL
                     FSASPDSLQTLVPELRKKYGRFVDLFSEGIIHIGKTDSPEYCNYLSQFLSDTMVQNAY
                     RLSQQKFQNISSIKVCLNEAFNRFHCYFPDKPVPKIYTFVSGYNLSLAVDDSILAIGL
                     DRYLGSNTVQYKLLGIPRYQVRNMRPEKIPSDAIRAWMYGSFSFHDSTDNLLSNLIYE
                     GQVMYLTKQMLPQESDSLIFGFTPSQHRWCLKNEKAMWTNLIENKLLFSTNTLDINKF
                     INDAPFTSGFPQESPGRAAVWIGYRIVESLMKNNDTLTLSEMMGISDYQKFLAMSRYK
                     PR"
     gene            23905..25383
                     /gene="dnaB"
                     /locus_tag="PK839_04475"
     CDS             23905..25383
                     /gene="dnaB"
                     /locus_tag="PK839_04475"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HOG72026.1"
                     /translation="MGKVPPQAIELEEAVLGAIMVEKDAVVIVSEILKPECFYKETHQ
                     EIFKAIQDLSSRLEPIDLFTVTEELRRQNKLEEIGGAAFLAQLTTKVGSAAHLEYHAK
                     IIAQKFIQRELIRVSTDIQNRSFDEDVDVDELLDYSEMELFKVAEGNIKSDTQPITTI
                     IKKAIKQIEEAGKREDGLSGVPSGFTALDRLTSGWQNSDLIIVAARPSMGKTAFVLSM
                     ARNMAVDHDVPVAFFSLEMSNLQLVNRLIVSESGIASEKIRNGRIDQSEWTQLTVKIK
                     NLSEAKIFIDDTPALSLFEFRAKCRRLKAQYDVGVIIIDYLQLMTGPSETKGNREQEV
                     STISRSLKAIAKELNVPIIALSQLNRSVETRGGYKRPQLSDLRESGAIEQDADVVIFI
                     HRPEYYGFMEDEDGNSTQGIAEIIVAKHRNGPVDDIKLNFRKEMAKFSDFDDVHYGDI
                     QPSHSPFTSSVPVTFSSKMNEDTHGDYDFLSGQGVDSSDIPF"
     gene            complement(25508..26224)
                     /locus_tag="PK839_04480"
     CDS             complement(25508..26224)
                     /locus_tag="PK839_04480"
                     /inference="COORDINATES: protein motif:HMM:TIGR02254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HOG72027.1"
                     /translation="MRIKFFSISNRYRYVFIDLDRTLWDFERNSLEALHELFSEYKLD
                     SVVADATTFSRTYVKHNNYLWNSYCKGELTKEVLRYKRFDATLSELGVNNHQLAAKLG
                     EEYLRILPLKTILIPNAKELLDYLAPKYGLMILSNGFHEVQVDKLKRCGLNHYFDRVV
                     TSELSGYHKPDPRAFGYALSSANIRKNQGIMVGDDLEVDIVGARKFGMDQIFFNPERK
                     PHGEKVTYEVETLKEITQIL"
     gene            26558..27682
                     /locus_tag="PK839_04485"
     CDS             26558..27682
                     /locus_tag="PK839_04485"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOG72028.1"
                     /translation="MNFSELNLNPHILEGISTMGYGNPTPIQEQAIPVILQGRDLIAC
                     AQTGTGKTAAFLLPILGKIAEKPTTEGYINTLIIVPTRELALQIDQQVEGFAYFISTS
                     SIAVYGGNDGNSWDRQRMALEQGADIIVATPGRLLQHIQMGYVKLDRLEHLILDEADR
                     MLDMGFFDDITAIISQLPTRRQTLMFSATMPPKIRQLAKTILDNPVEISLSVSKPVES
                     ILQAVYMVEDSEKIGLLQSLVAGKEYVQSVLVFASKKMEVKDIERKLVRESIMARAIH
                     SDLEQSQREQVLRDFRNRKFQVLVATDIVARGIDIEDIDLVVNYNVPADPEDYVHRIG
                     RTARAQSDGVAITFVNKKERPQLARIEKFLGETIFRVSHS"
     gene            complement(27730..28761)
                     /gene="rlmN"
                     /locus_tag="PK839_04490"
     CDS             complement(27730..28761)
                     /gene="rlmN"
                     /locus_tag="PK839_04490"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HOG72029.1"
                     /translation="MEKQNNKVPLIGFTPQELCSTMQEYGGTSFQAESLMRWIYRKRE
                     KDISTFSDIPKAILQSVEQSYTVGLYPPKESKRSDDGSVKYLFANGQNQVFEAVAMSS
                     GNRHTLCLSTQSGCRMGCRFCRTGSIRFNGNLSAGDIINQVLSIPDSKRLNRIVLMGM
                     GEPLDNLKVVNKTLEILTADWGCALGKSNITISTVGLLSELEAFLKSPGCNLAISLHS
                     PFPDERAMLMPVERTNPILEIIKLLKHYPLKKPLRLTFEYVALGSLNLSKNHARAIAT
                     LLSGLSCHLNIIPWNRHNAATFQSPTSDELTNFMELLNDAGIPTNLRKSMGGDIDAAC
                     GQMAGNAPH"
     gene            29104..30117
                     /locus_tag="PK839_04495"
     CDS             29104..30117
                     /locus_tag="PK839_04495"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HOG72030.1"
                     /translation="MSTNAEIEKKPSIAVVGSGSWATALAKVLHETQDSIGWCLRDPE
                     LIGYIKKYRHNPNYLSAVEFDVKKLYLSVDINEVAKLYDILLFVIPSTYFQAWLEPLS
                     VSLEGKFIISAIKGIVPGMDCTIAEFFNQQYGVPFDRIGIVSGPTHAEEVALERLSYL
                     TISCKDKLQAKHVASYFETHYIKTNIGTDIYGTEYSAVLKNIYAVASGIAHGLNYGDN
                     FLAVLISNAQREIERFLKKTYPSKRRLSTSVYLGDLLVTCYSQFSRNRTFGTMIGKGY
                     SVKSAMLEMSMVAEGYNAAKCIWEINKKYNVKMPIANAVYNILYENISPAIEMKLLTA
                     KLR"
     gene            30147..31493
                     /locus_tag="PK839_04500"
     CDS             30147..31493
                     /locus_tag="PK839_04500"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HOG72031.1"
                     /translation="MNQLKVDLSNIYTQVAKEEVFALKTKIAQNQLLLEQGTGKGSDF
                     LGWLNLPSSITKEQLYDIQHTASELRAKSDVVVIVGIGGSYLGAKAAIDSLSSSFTQL
                     TEDVLPKIIFAGNSICEDYHAELLELLETKRYSIVVISKSGTTTEPAITFRVLKQHLE
                     RQVGKTEAASRIVAITDLSKGALRKLADSEGYRTFVIPDDVGGRFSVLTPVGLLPIAI
                     AGIDIVKLVEGARKAMDDTSSSVAFDQNPACIYAAARNALYQKGKKVEILVNFTPRLH
                     YLTEWWKQLFGESEGKEGKGIFPAGVDFTTDLHSMGQYIQEGERMLFETVLSVEKPSH
                     RLVVPDDPDNLDQLNFLSGKRFSEVNLMAQMGTIAAHSDGGVPCISISIPELSPYWVG
                     YLFYFFERACALSGYTLGVNPFDQPGVEAYKRNMFALLGKPGYEADGRKLREKLGL"
     gene            complement(31727..34135)
                     /locus_tag="PK839_04505"
     CDS             complement(31727..34135)
                     /locus_tag="PK839_04505"
                     /inference="COORDINATES: protein motif:HMM:TIGR03296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M6 family metalloprotease domain-containing
                     protein"
                     /protein_id="HOG72032.1"
                     /translation="MIKHLQSIRKLAFTAIIGVMTLTGYAVPKHNLPITITQPDGTKI
                     SCFVSGDEFHNWLHDAAGYTIMRNPANGFYVYAVEKNGKLTSSDYVVGKVNPRNSKDL
                     KPYVNISRNEYLAKRHRKQTDNHHVNLDITKGTSGIAPLRAVKGVTMNNIVVFIRFKD
                     DVVADENISYYENVYNGSSASVANYYSTVSYGKFSVSSTFYPQLNGTLVTWYVDENNR
                     NYYQPYDATTNPNGYNPDASNYNDPTSSTYREHTLLKKAVEAVGASIPTGINLDINGD
                     NYIDVISFILAGSNDSWNDLLWPHQWSLWSQSAFINGKQVGDYTLQLQYFGGSQIDLG
                     TLCHEMFHAVGAPDLYHYDDDLNLSPVGYWDIMEGTLDYPQNMGAHMKYLYGGWIDQI
                     PEITTSGTYTLSPLATSPTGNSYKIPSPNSYSEYFVVEYRKKESYDSDLPGNGLLVYR
                     INSKLEGVGNADGPPDEVYIYRPNGTPTLNGSINSAHFNSSVGRTSINDNTNPSSFLT
                     DGTNGGLKISNITAAGETISFDATIDFIPYVVLKNDRGYATAIGDESTTLTVATRFTT
                     QDLASLVGRKIKMVDFYIRGGENGSDVTTNETVKVWEGGTYGNPGSLIYSKNISSEVV
                     PDQWTSHTLSQPIVIQPNKEYWVGYTATASGGYPFATDGGPMVAGKGGWIYINNQWYE
                     LININSQLNYNFLVRAVVGSETNDAETLTANAVNFKVFPNPANASSRVSITLPMAGRV
                     AVDLYNMLGQRVGGSMPTHYEAGVQSLPLSGNELSNGIYLLTVTYDSGTHHTVETQKI
                     VVAR"
     gene            complement(34212..34796)
                     /locus_tag="PK839_04510"
     CDS             complement(34212..34796)
                     /locus_tag="PK839_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72033.1"
                     /translation="MAHNRNKAALSIALSFLLFVTTACYSGKKTADYSTVTNNAFAFV
                     EYYQTTDVQVMAGEYHGGRRIDSPTYAFNPEDRTLNIYRYPDGLQPDSAIVVLGVGKI
                     LKNAAGSGVSSFLVGANQLPFSYNDLTITSLSNNKLFLTYQNKSITLKAGEEWKHYTE
                     KTDTLKLQGLPVIKTMQTSTIRFHGFLSRNILKK"
     gene            complement(34894..36228)
                     /locus_tag="PK839_04515"
     CDS             complement(34894..36228)
                     /locus_tag="PK839_04515"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HOG72034.1"
                     /translation="MKHFYLTATLLLLFTTATFAQDTLNPDTDIIPEDTLLIQYINGN
                     LDSLLNLWYVQQTVDSNSLVANIEDDSVGLTGYPDSFYIKRLQSINSLIELPFNSIVR
                     NYINVYTQKKKSQVEVMLGLTDYYFPIFEEILDKYQLPQELRFLPVIESALNPKAVSR
                     AGATGLWQFMYGTGRRYNLTVNSYVDERRDPIASTHAAARFLRDLYAIYGDWTLVIAA
                     YNCGPGNVNKAIRRSGGKRNYWDIYYYLPRETRGYVPAFIAASYTYYFSHEHNLVPQP
                     INIPPTTDTIVINDMLHLQQVSEVLDIPLDMLRDLNPQYKLDVIPGKFKPYALRLPLD
                     GVGPFIDREKDIYAYKDSLFFNPRVTISPNKYTSSSQYDIPSGSIRYVYRVKEGDVLG
                     IIAEKYGVSVGQLRSWNNLRGNIIRIGQRLVIYIPERTANKMGISGDKSQTS"
     gene            complement(36467..38392)
                     /locus_tag="PK839_04520"
     CDS             complement(36467..38392)
                     /locus_tag="PK839_04520"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOG72035.1"
                     /translation="MKKILLLLIFLLPMLLSAQSPKNLSTKNKRAVARYNEALAAYNR
                     YNLDEAERLLKESIKIEHNFIEAYLVLTQVYSEKGEPEKAISSAEKAVSINNNFFPKT
                     YFNLGVLNFSIGNYQKALEYFEKYLTYHETSGSMVELARNNIERCRFAIHAVANPVPF
                     NPANIGDSINTTLDEYWPSLSVDERTIVYTVRLPKEPEVGIKGTKWQEDFYTSTRNKD
                     GTWSKGIPVGSPLSTVFNEGAQSLSSDGKTMYYTICQGDCDLYFSTINSDGSWGHPLK
                     LPPAINSEKSSEKQPSISPDGQMLYFVSNRPGGLGGFDIWVSVKNPNEVWGNAVNLGQ
                     PVNSPGNEQSPFIHFDNKTLYFSSNDHIGMGGQDIFVSHLTDSGKWSEPLNLGYPINT
                     CKNEEGLVVNAIGKTAYYSSNYIPDKGMDIYTFELYPEIQPIPSSYVTGTIRDYTTLK
                     PVEAHISLIDLQSKRETMNSYSSVTGGFMVCLPSDNSYALFAQAPGYLFYSENFNLKG
                     LYSFDKPYHLDVLLKPVRQGEVTIMRNVFFETDSYELLPESTVELDRVVETLSQNPTI
                     KVEIGGHTDNQGAPDYNQKLSERRAKSVVDYLLSKSVSPERVSWKGYGESQPVAPNSS
                     PEGRAMNRRTELKILEN"
     gene            complement(38564..39202)
                     /locus_tag="PK839_04525"
     CDS             complement(38564..39202)
                     /locus_tag="PK839_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010265750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HOG72036.1"
                     /translation="MGDHTTINPELESGKVLPLMEEFYTLQGEGFQTGRAAYFIRIGG
                     CDVGCSWCDSKQSWNSRLFPPVSVDEIVQRAIAYPARAVVVTGGEPTLYPLGYLCSEL
                     KKHNIQTFLETSGAYPLTGQWDWVCLSPKQQQPPVDLNYSKANELKVIIAKQADLCWA
                     EANAQRVRPDCMLFLQPEWSVHESITPLLVDYIKQNPKWRISLQAHKFMHIP"
     gene            complement(39204..40985)
                     /locus_tag="PK839_04530"
     CDS             complement(39204..40985)
                     /locus_tag="PK839_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018919111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HOG72037.1"
                     /translation="MKSITSRIILLQALVSLSLFCYAQETVTIKILETSDVHGAIFPY
                     DFIGNKPMESSMAQVYTYVKKERENSQQQVILLDNGDILQGQPTVYYSNYIDSSSCNI
                     VSRVFSYMGYDAATVGNHDIETGPLVYRKVERELAIPWMAANAINTITGKPAFKPYTI
                     IKRSGLKVAILGLITPGIPSWLPRNLWPNMRFDDMVESATYWVQQIQEKEKPDVLVGL
                     FHSGYNSSYGGANAETPLNENASLLVARKVPGFDVIFIGHDHDKLITKVENVQGDSVL
                     VLDPTSSARYISEATIQLSLDKKKRVVSKNITGTLVETRRFSPDSAFMAEFKSCYQKV
                     DSFVNQPLGEFTQTISSANAYFGPTAFMDFIHSAQLSITNADISFAAPLSFVSTINKG
                     IVRVSDMFKLYRFENFLYTMNLTGAEVDAYMEYSASLWFNTMKDANDHLLRFRQNADG
                     SMATDNRGKVELASRYYNFDSAAGIVYTVDVSKPDGNKVTILSMVNGEPFDTAKTYRV
                     AINSYRGNGGGGHLTKGCGLSAEDIQKRIVASTDKDLRFYIMKFIEQQKVITPKSFGT
                     WKIIPEEWAASGAARDHKLLFSYTGND"
     gene            41548..42306
                     /locus_tag="PK839_04535"
     CDS             41548..42306
                     /locus_tag="PK839_04535"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOG72038.1"
                     /translation="MNTYDLKGKVAVVTGAMRGIGKGIALLLARNGARVVVTDIDKAS
                     CEQVVKEIEKAGGTGVAFKLDVTNEQEIQDVVKSVHAQFGRIDILVNNAGIFIQEELD
                     GMDTAKIDKELGVNLRGTLLCTKSVLPIMKQQHYGKIVNLASIAGFVGFEVASVYSAT
                     KGAIVNLTRELALELGKYGINVNAVAPGVIETPMTDGLLEDKNVAAALLSKIPYGRTG
                     KPEDIANGVGFLVSDDSSYITGHTLVIDGGWLTM"
BASE COUNT        10673 a         9158 c        10196 g        12385 t
ORIGIN      
        1 ccagcgagca attccttctc cctccacaag aaatggttga gttttttagc cctttgctgt
       61 ttgggagaag ttgcctgtaa aaaataggaa cgatgcttca ggaaatttca gtggcaatat
      121 ttacttgcag cttgtgtcgt ctgaaggtgc tattttagct gcatgttgtg aagcttggcg
      181 tagtagccgt ttagccttag cagttcttcg tgccttcctc tttcaactat ttcaccctcg
      241 tgtagaacgc atatcatatc ggcatttcgg atggttgaaa gtctgtgggc aataaccagt
      301 gaggttctgt gcttcattag gttctcaatg gcatcctgaa ccagcttctc cgattcggta
      361 tcaagggcgc tagtagcttc gtcaagaatc attatgggtg ggtttttaag aatggcccgg
      421 gcaatgctga ttcgctgacg ttggcctccc gagagcttgc taccccgatc gccaatgttg
      481 gtgtagtatc catttggtgt agccatgatg aagtcgtggg cgttggcaat ttttgctgca
      541 gcaatgacgt cctcctcggt tgccgagggc attccaaagg caatgttgtt gtaaaaggtg
      601 tcattgaaga gaattgggtc ctggtttaca tttcccataa ggtttcggag gctttcgaga
      661 ttgcaatcct taacatttac cccgtcaact agcacttcac cttcggttac atcccagaaa
      721 cgtggcagca ggtccaccag ggtgctttta ccagaacctg actgccctac cagagccacg
      781 gttttcccct tttcaactac aaggttgata ttatggagca caggttcact ttggtacctg
      841 aagttgacat tacgaaactc tatggccgag gagaactctg tcagcacttt agcttcgggt
      901 tttatcttaa ttggattctc ggccgaaaga atagtatcaa tgcggtcggc agaggccatt
      961 cccttcagca cgttgtagta agcattggag aacgacttgg cggggttgat aatcatgtag
     1021 aatatgccaa tgtaggctat tagcgcctcg ggggttagcg tcccttttcc cttaaaaatc
     1081 agatttcccc caaaccatag caccaacaca actacgattg ttcccaagaa ctcgcttagg
     1141 ggtgaagcca gatcccttct gcgccacatt tttttcatca acagggtgta gaagttattg
     1201 acgctttcaa aacgggagat aacttttttt tcagcattaa aggcttttac aacccttagt
     1261 ccgcttagtg tttcctcaat cagggtaagt atggccccca ttttacgttg ccccttaaac
     1321 gaagtctttt ttagggtttt gccaacccgg ccaattacaa ccccggttag gggtagtagc
     1381 agcagcacaa aaagcgttag ctgtgggctt atgtagatta accctataag gtgaatgatg
     1441 atgatgatag gatctctgaa tatcatttcg agcgaacgaa tgatggaaac ctcaatttcg
     1501 ttcacatcgt tggtcatttt gctgatgaca tcaccctttt tctcctcggt gaagtatccc
     1561 aatggcaggt caactacctt cctgtaaagt gtgtttcgta tgtccctgag cacacctgtg
     1621 cgaatgggtg ccaggtagta aagcgacatg taggtgaaaa agttttttag gaacgaggcc
     1681 gtaagcacca gtcctatgac gaatagcatg gctgttgacc ggtcgtggtt gcgaatcatc
     1741 tcgctaagca tgtagtttag ggtgtcgttc agggatttaa tggtcatggt aaactccggt
     1801 ttcacctcaa ccagcggaac agttccaaag agcagctgca ggaacgggat gagcatggcc
     1861 aatgagaaga gcgagaatat ggcacctaaa atattaccta caacgttagc tacaacatgc
     1921 catttatatg ggatgataaa tttgagtagg ctgaaaaatt tcttcatttg agatttttgt
     1981 gaaaaagcgt gtaaaggtaa gtttaatatt tcagaaaatt tagaagaata cctacttcac
     2041 tccgaggtag ttgaacgggg taatactttt cagctcggtt ttaaccctac tatctatatc
     2101 cagctcatca atgaaggtga ggaaatcctt ttgcgtaacg tttttgttgt tgcgggtgag
     2161 ctttttaaga gcctcatagg ggtttggata tccttcgcgc cggagaatag tctgaatggc
     2221 ttcggcaaca accatccagt tggtttccag atcatttttg atgacttcgt tgttgataat
     2281 cagcttgttt agccctttga caatggattg aatggcaatc tgaatgtggg caaatggaac
     2341 ccctacgttg cgcagaacgg tggaatcgga aaggtcgcgc tgaagtctcg aaatgggcag
     2401 cttggttgcc atgtgatcga gtatggcatt tgccaagcct aaattgcctt cggcattttc
     2461 aaaatcaatg gggttaacct tatggggcat tgccgatgag cccacttcac cctcaacaat
     2521 tctttgcttg aagtactcca ttgaaatata agcccagaag tcacggcaca ggtcaatcag
     2581 gatggtgtta attcggcgaa ggcagtcaaa aagtgctgca aggttatcgt aatgctcaat
     2641 ctgcgtggtg gtttgagagc gctttaaccc caaagtattc tcgacaaagc cattggcaaa
     2701 tgccacccaa tccacctcgg ggtaggctac caaatgagcg tttaagttac cggtagcccc
     2761 gccaaacttg gcggaatatg gaattgacct gagctgcaat agctgaacct ctatccgctc
     2821 agcgaagacc cgaatttcct ttcccaacct ggtgggtgaa gcaggctgac cgtgtgtgtg
     2881 cgctagcata ggaatttcag cccattcgtt gctcaggtcg tgaagtttct ccaggagttt
     2941 cgagagggtg gggtagtagc agctattcat gaaatcacga attgatgccg gaatggccgt
     3001 gttgtttata tcctgcgagg ttagcccaaa gtgaataaac tccttatagt ctgaaaaccc
     3061 cagctcatcg aatttttccc gtaaaaagta ctcaaccgct ttgacatcgt ggttggttgt
     3121 gctctcaata ttctttattc tggctgcatc accctcagca aatttttcat atatacctct
     3181 aagcttatcg aaatttccgg ttcgaaactt tttgagttgt ggaagcggca agttgcaaag
     3241 ggcaataaaa tactccactt caacccacac cctgtacttg ataagggcga actccgaaaa
     3301 atagttctgt agttccttgg ttttgctgta gtatcttcca tctataggcg atatggcgtg
     3361 tagggcattg agcgacataa gtatagtttt gaatttttac aaaattacta aacattagct
     3421 attatgtagg gtttcctaca agttatattt cagctaccaa tattttacta actttcggaa
     3481 cttctgatta tggaaaaact ggttaaaatt tcggccattt cctaccttaa taccgcacca
     3541 tttgcatacg ggttaaggca ttcggacata ttctctaaaa tacaacttct ttccgattac
     3601 ccctcagaat gtgcccgtaa gttacaatac aacgaggtgg acatcagcct tttgcctgta
     3661 ggggctctac catctctttc ccattacgag gtaattaccg atttttgcat aggagcgata
     3721 gcggatgtgc ggactgtagt tttgttgagc aacagtccac tccatcgtat cagaaagata
     3781 tatctggatt accaatcccg aacatccgtt cagctggtta gaattcttag caggtactac
     3841 tggaatataa atcccgagtg ggttccgctt tctccggagc aggactactc tactattcct
     3901 aaagattatg gtattgtgct tattggcgac agggtttttg aggtggaaag caggtatcag
     3961 ttcgtttacg acctttcggg cgaatggggc aaatttacag gattaccctt tgtttttgcc
     4021 gtttgggctg cagcctcgca acccaccgac gatttcaagc atcagcttaa ccatgcgcta
     4081 cagcttgggg ttaattccat ccctcaatcc gttgatgagt ttgcccttaa gaatattgaa
     4141 agggatgagg ccatccgcta cttaaatcaa aatatttcct accctcttga cgctgagaag
     4201 cgcaaggcca tttccctctt tttaagttat atgagccaaa accagtagta gtgctgcata
     4261 gctatcagcg tcaatggcta tatttacact aaatttggag aagattcgtt aaagtaaaag
     4321 taccccaaaa tgaaaagatt aaaagacgtg gctagtagat tggcatgcgg gattgtcgct
     4381 gtgttcctgc taacaattcc gtttcacctg gtttatgcag gtgagccggc tactaatagt
     4441 ccaactgtgg tttataagat agatattcag gagaacatca tgcctgccgc ctggcggcat
     4501 gtaaaaaggg gttttgagga ggcagccaga ataaatgccg acattatact gatacacctc
     4561 aacacctatg gcggcatggt ggatatggcc gattccattc gtacaaaaat tcttaacagc
     4621 aaaattcccg tttgggtttt tattgacaac caagcggcct cggcaggagc cctgatttca
     4681 attgcctgcg atagtattta catgcggaaa ggtggaagca tcggcgctgc aacggttgtt
     4741 gaccagtcgg gtaatgtggt tcccgacaag ttccagtcgt tcatgcgggg catgatgcgg
     4801 gctactgccg aagccaaggg taaggacacc atagtaacca aaggcgacac cttgttgaaa
     4861 tggcggcgcg atccgagaat tgcagaggcc atggttgacc cgtcaatttt tattgaaggg
     4921 ataattgact ccggtaaggt tctcactttt accgccgatg aagctatact acatggctac
     4981 tgtgagggta aggccgaaac cgttgatgaa gtattagcca aagcaggtgt tgaaagctac
     5041 aggctggagg agttcacccc cactttcatt gaccggatta taggtttcct tatcaacccc
     5101 gttgtatcag ggattctgat aatgctcatt attggtggta tatactttga gctgcagtcg
     5161 cccggaatag gttttccgct agtagttgct gtgcttgccg ccatcctgta ctttgcacct
     5221 ctttaccttg aaggccttgc ccagcactgg gagctggcac tttttgttgt tgggcttatc
     5281 ctgctattcg tagaaatttt tgccattcca ggttttggag ttaccggaat cagcggaata
     5341 attcttatgg tggtcagcct caccctggcc atgattgata acaacctgtt tagaggagtt
     5401 ggctcattca acgtcttaat cctgttaaag ccactggcaa ttgttttagt tgctggtttt
     5461 ctgggcttct taggaggact ttacctgagt aaaaagtttc taacctcctc catgttttcg
     5521 aaccttgcgt taaactctag cttgtcaaaa gaggatgggt ttgtgggagt tgacaagcaa
     5581 ataaagacga aaattggcct tgagggcatt gctgccacca tgttgcgtcc ttccggtaag
     5641 gttaagattg gagacgattg gtttgatgcc atttcagagt atggttatat caataaaggc
     5701 gagcgggtta gggttacacg tgatgaagcc gggcagcttt acgtggtgaa agccgaataa
     5761 ttttgaatgt atcgtggaaa atttctcagt aagggatttt ttaccaagcg attttgaagc
     5821 attatcgcta gtttggagcc taacgggttt gggaaatcct gctcgtggtg ataacctgga
     5881 aattatcatg cagagcatag ctatgggcgg gaagctgctg gttgctacca gctcccaggg
     5941 ccttgtggtg ggtactagct ggatgacctt tgacggtcgt agaatacacc ttcaccactt
     6001 cggcgtacat cccagctacc agcgaagggg gattggcaag atgctggcag ccgagtcact
     6061 aaggtttgtg aagcaaaaag gctaccaggt gaagctggag gttcatcagg gtaacctggc
     6121 tgcaatcaac ctttacaagc agtttggctt tcaacgtctg ggtgattacg atatctacat
     6181 cattagagat ttaaccgtga ttgatagtgt gctctagcct tacccgttag acttagcaca
     6241 gcagtgcaag ctactcattt ccaaagaaaa tgtcgttaaa atttaccatg taaacctttg
     6301 aggtagcacg ggtaacagcc gtgtagagcc agcggaggta ctcacggttg atcatgctct
     6361 ccctaaaaaa tccctggtca ataaacacgt tgggccactg tccaccctgg gccttatggc
     6421 atgtaactgc ataggcaaac ttaacctgaa tagcgttgaa gaaaggatcc tccctaattt
     6481 tttgcagcct tgtccggtag gttttaatat gctggtaatc ctgtgctacg gctgcgtaaa
     6541 ggtttttcat ctgctccgag ccgagcgagg gtccgtcaat ggtgagtgag tcgagaataa
     6601 tcctggcatc tacctcctgg ttatcctcgt cagtcagctt cagggtaaca tcggcaaaac
     6661 gaaagccgta aaggttgtaa tgtttaccta tgcgtttaac ctctacaatt tcaccgttgg
     6721 caataaaagc aatgtttggg ttatctgcca cccagtagta gttgttacgg gccaccataa
     6781 ggtagtcgcc taccgaaagt tcctcctcgt tgaacagtat ccgtgaacgt atggctatgt
     6841 tgtagcggtt tgcctgcttg ttggagtaac agatgaccac gttgctgttt ctattgctac
     6901 ggctgtactc ctccgtcagg tattccatta tggagcctcc atcaatcctt tcaatatcct
     6961 taaaacgagt tgaaacatca aatttaggtg tagagatggc cgttgagtct aaaatctgcc
     7021 ttatgcgggt tgcattgaaa aggattcctg aacctaaatc ctgcctaaca acctcgttta
     7081 gaaaaatctc ctgaacatca aggttgaaca ttgccagctc tcttgcatcc agcgcagggc
     7141 taatgtccaa tcccacaggt ggcagttgag ccgtatcgcc cacaactatc agcctgcagt
     7201 ttttcccctg cttaacaaac tccaccatgt cggaaagaag cttccccgac ccgaaggcgc
     7261 tatcctcaaa tgaggagttg gaaatcattg atgcctcatc tacaatgaaa atggcgttag
     7321 taagcttgtt taagttgagc acaaatttgc cgataccctc ggatatactt ttctgccggt
     7381 atatgcattt gtgaatggtt agcgccttga tgcccgaata gtcagtaaga acctttgctg
     7441 cccttccggt tggggctagc agctcgaatg gcaccttcag tttttgcaga gtctttacat
     7501 aaccggctat cagggatgtt ttacctgttc ccgcataacc tttaataagg agacatttcc
     7561 cagaatctgg gtttagggtg aagtccgaaa ggatgtctat agcggctgcc tgcccggatg
     7621 ttggtgtgta tcctaaatag gacttgatat cttcctgaat ttgaattttt aacaccgcgg
     7681 tactacattt tctgatttaa aaggtttaat ttggctttat gtttttaaat ttgcgcaaac
     7741 ttatactaat tacatctttt tgaaaaactt ttctttagga ataatcattc atgcaatcat
     7801 cctttcatac caggaaagag agtatgaaga tttttttggt tccgaagaag catattaata
     7861 cacacctctt tggaggcact ttatcaaaca aggcaaatag gcatgttgaa acccctgaag
     7921 cttatagacg aatcgttcga cagggataga acgggcagct acgatttatc cgttaccatt
     7981 tccataaatg gcttttcatt tgctgttttg gataccctac gaaatacctt cattgttttg
     8041 gtttcaacac ccaaaagtga gcagcaggat tttgaggagc tgttcaacca ggccatggtg
     8101 gattatccgt ggttggccgg gagcttcaaa catgtttacc tttctttctt ctcaccatac
     8161 tttacgcctg ttccagggaa attcttttcc gagcaaaaag ccaggcaact ttttgagcta
     8221 gtacatccct tgcccgatgg tttcgagcta catttcaccc caaattgcaa ccatactgat
     8281 gccaccctac tttttgccct tcccaattct gtggccaact ggtggttgaa gctacatcca
     8341 aaggcatcat ttcttcaccc aattgctccc ctgcttagtt cacctgccgc aggctcaaat
     8401 gttttactaa gtgttgattt taacgagaat gattttcttg tagctctaca tgatgggatg
     8461 aagtttgcgg gcgcaaatgc ttatacgggt cggcatccaa atgacgtgat ttactacctg
     8521 ttagcctttt gcaaggagtt gggctacgac tcacgaaatg taaaggtttc cattacaggg
     8581 cattcaccca tgctagcaga gttaaacatg ttggatggct acttcgacag ggttagctca
     8641 attgcccagt atccaaaggt tatgctgttc agctaccagc tcatcagcca tagaagcgag
     8701 tacttctcac tttttaacct cggggcatca tgcgaatagt aggcggctca ctaaggggac
     8761 gaaggtttga tcctcctaaa aactttgggg ctcggcctac tacagatttt gccaaggaag
     8821 gtcttttcga cgttctgagt aactactttg attttgagga gcttacggtt ttagatttat
     8881 tttccggtac gggcagcata agcctggagt ttgcctccag aggggcacgc catattgatg
     8941 ccgttgaaaa aatttatgct catcagaagt ttattaagga tagtgcgctc aggctgggcg
     9001 ttacctccat atcagtaatc agacaggatg tattctcgtt tatcagccga agcacctgtc
     9061 gctatgggct aatatttgct gatcctccct acgatatgga gggcatcaac acgctcccag
     9121 agttgatact ttcaaaggat ttgcttgacg aagatggatg gttggtggtc gagcattccg
     9181 accggtacag cttttcgggg ctaaagcagc ttatagacca ccggcgttac gggagtgtga
     9241 attttagctt ttttagaaaa aatatttagg ggaatcttgt attattgctg ggaatgttct
     9301 acctttgcaa tgttttgtta taagctattg gtccggtagt tcagtttggt tagaatacat
     9361 gcctgtcacg catggggtcg cgagttcgag cctcgtccgg accgcaaaaa acctcgggat
     9421 ttcccgaggt ttttgtattt aattgcttgt agaaaagtca ggttaaccgt accaagcgaa
     9481 aggttagcct agttgcagtc cttgcactcc tccaggttcc ggttgtatat agccattgcc
     9541 ttattgctca tacccatgct ggagaatcca ccatcgttgt atatattctg catggtgatt
     9601 tttcgggtga ggtcgctaaa tagggttatg caaaagtcgg cacaatcgtc tgcgttggcg
     9661 ttgcctattg gtgacatacg gttggagaaa tcgagtaggg catcaaaacc cataacaccg
     9721 ctaccggcag ttgtttcggt tggagattgc gaaacggtat taattctaac accgcgctcc
     9781 ctaccatata tgtaaccgaa gctgcgggca atcgattcta aaagggcttt tgcatcggcc
     9841 atgtcgttgt aaccgtaaag tgtgcgttgg gcagcaatat aggagagtgc aacaattgag
     9901 cctccatgcg taatagcatc ctttttccag caggtttgaa tgattttgtg aaaagatatg
     9961 gcggagatgt ccagcgtttt ttgcaggtag tcgtagtcca gatcatcgta cgttcttcct
    10021 ttcctgacat tgggcgacat cccaatggag tgcaggataa agtcgaactt tccaccaaaa
    10081 tgctccatgg tcttgtctgt caagttctcc aagtcaggaa tgcttgtggc atcagcaggg
    10141 ataataatag cgttacattt cgcagcaagt tcgtctatgc tgcccattct catggcaaca
    10201 gcggtgttag agagcactat ttctgcaccc tcttcgtgag ctctctcggc taccttccac
    10261 gcaatggatt tctcgtttaa ggcgccaaaa attagcccct ttttcccttt tagtaagttg
    10321 taggccatat cacaaaatta ggtttaacaa atactaaaca ataaaggtaa atttttgttt
    10381 agtttagttt agtttaggct accgtttgat tttagcagct ctctggcatt taaaattgca
    10441 gcatcggaca tcttttcccc actaagcatt ttggcaattt caacaaccct ttcttccggg
    10501 gtgagtagtt ttattcctgt tgtggagcgc agctcctgat tttccttgta aaccaaaaag
    10561 tgtgttttgc ccttagccgc tatttgggga agatgggtga tatttattac ctgcatgttc
    10621 ttagccatgg tgtggatgat gctgcccatc ttatcggcaa tttctccgga aacaccggtg
    10681 tcaatttcat caaatattat ggttggaagt cccgaggctt taaccagtag cgattttaag
    10741 ctgagcatga ctcgcgacag ctcgcctccc gaggcaattt ttgaaagctc catgggtgga
    10801 atctggctgt tggccgagaa gtaaaaggtt accctatcgg ttccccaagg ttggaattcg
    10861 ttaacaggct gaatatcaac cttaaaaatg gcatgcttaa ttccaagttg gataagcagt
    10921 tctgttatgc tttgttcaat gggcgctgct gcttttcgcc ttaggattga cagctcgtct
    10981 gctttctttc taagttcatg tcctctacgc tccagatcct tgcgtcttgt ttccaggtta
    11041 aactccatcc cagaaatgtg gtttaccctt tcctctatcg agttacggta ggcaatcagc
    11101 tcggtcatgg ttcccttacg gtgtttttgc agtagggtgt agagcaggtc gatacgctgc
    11161 gatacgctag ccagcagctc gtcgtcaaca ttaacctgtg agctgcgtcg ctccgcttca
    11221 tctacaatat ccctcagctc aatgcggcag ctttccaaac gatgttccag ctcatcggca
    11281 gcaggatagt agggcgatat ttttttaagg cttgcagaag cttcttttag cagggctatg
    11341 gccgatgttt cgtcgttgct agctgcattt atggtgaatt ccaatgccga tttaatttcg
    11401 gttgcgtggg taagttttgc ttgttcaatc tccagttcct ccagctcgcc ctctttaagg
    11461 ttagcagcgc gtagctcctc cagctggtgc tgcaggtagt caaaatctcg accaaaattt
    11521 gctgcttcgt cttcaagttt ttttagctca gcctcaagct ggcggtagtt taagtacgtt
    11581 tctaggtagg cggattgcaa gtttttggga atggcgtagg tgtcaaccac attaagctga
    11641 aagcgggagg ttcccagcag gaggttttga tgctgtgaat gaatgtctat gaggcggtca
    11701 cctagttcct taagaagtgt gaggttaacc ggaatctcgt ttatgaaagc ccttgatttg
    11761 cccgattcgc ctatctgtct gcggacaatg gtagttgggt tgtaatccaa atcgtttact
    11821 cggaaaaagt catcaaggtc gtagctgctg atgtcaaact ccccctcaac tatgcagctt
    11881 tttgactggt ctttcagtat agacgcatca accctttggc ccagaatgag catgagcgct
    11941 cccagcagga ttgattttcc ggctccggtt tcacctgtaa tgatattcag gccaggttga
    12001 aactctatgg tcagctcatc aattatggcg tagttttgta tgtagagttt ccgaagcatc
    12061 ggcacaaggg gtttaggcta gctattcttt tgctccagta ttttctggta cttgccagcg
    12121 ttggcattgt caatttcggt aagaatgtta actaccctgg ccttttcgga ggggaacgat
    12181 tccgagaaaa tatttaccag ctcgtcactt ttggcgtcaa agaaaagctg cagggcaaag
    12241 aggtaagggt cgggcttttc gcggtatacc ttttgaaggg tggccataca ctcagctatt
    12301 tctgctctac cctccactgc cttatcgctc atccggtcta gtcctaagcg atggtaacgg
    12361 tagagcacct ctcgaagagg ccggtatttg gagtttagaa ggttttcaat catccagtac
    12421 ctgttctttc ggtttccctc gtaggctttc catccccgct caggtgcatt ctgggcgttg
    12481 ttcactactt tttcggcttt aaggagataa tcggtaccgc caagcatgga aaatgaatcg
    12541 tagtcaattc ctagtataat gtaagcgtag aacgccagaa tggatgaaag attgctgaga
    12601 tggctgtttt cggagaactc aatcttgtca aattctctat agcgaaagtg caggttgtta
    12661 tcctgaaagt tgagcatggt ggtgttgtag gacgagttga ataccggccg gcgcgattgt
    12721 acctggaggg ttcccttaaa ttcgtcagat cccagctgtt cggtgatgtt aagcattatg
    12781 ctgcattcaa tacgctcgtc gaaaccgtaa acgtggttag tccatgccgt gttgttcatg
    12841 aactcgtaga tggctgtttg gagcgtttga aaaatagtgc gattagtgcc ctgaattttt
    12901 tgggagttaa tctgcacatt gcaccttaac tcctgtccat cgagtaccag cggggcaatg
    12961 agcagaaggg ttaacatcag cttagcaaaa gacttgtgca tggcgattcc cgttggtttg
    13021 tactattttg cttgttgaag caggtcaatt atggcgttgg ctatatcttg ggctacctct
    13081 gatttaggct ttagctcaaa ggatttttgc ttaccattgc ggtcaattag ggtaatcttg
    13141 ttggtgttat ggccaaaacc agcgccggca tcgttcaggg agttaagtac tatgatgtcc
    13201 aggttttttc gggttagctt gtcaatggca ttttcaacct cgttgtgggt ttcaagggca
    13261 aaaccgacga tgatttgatt ctgccgcttc atcttaccga gtgtttccgc aatatctgtt
    13321 gtcggctcaa gttgtaggtc aagattttcc ttcccccttt taattttatc ggttgaggtg
    13381 ttagctggag cgaaatctgc cactgctgct gcaagaatgg caacctgaca gctgtcaaat
    13441 tcattttttg cctgctccag catttcggag gcggttttaa ccctgacaat tttaaccgaa
    13501 ggatgttgtg gttcaacagc tgccggacca gtgatgaagg taacctgtgc tccttgttgc
    13561 gcaatggcgg tggcaatggc agttcccatt tttccgctgg aataattacc aataaagcga
    13621 acgggatcga tgggttcata agtaggaccc gaggttatta gtaccttgta tccattaaac
    13681 cggttagccg ccaaaaaaag gttgactacg tgttgtagaa tattttcggg ttcctccatt
    13741 ctgcctttgc cggatagccc gctggctagt tcccctgtag cagcctcaac aaaaatattc
    13801 ccgtaacttt taagaatctg aatatttttt tgtgtagcgg gatggctata catatccata
    13861 tccatggcgg gagccagcat aacagggcat ctggccgaga ggtatgtggt gagcagcagg
    13921 ttatcggcaa caccacttgc catttttgcc atggtgttag ccgaggctgg agctatcaga
    13981 aacaggtctg cccaaagtcc caaatcaaca tgactattcc acccgccatt ctcggggtta
    14041 aagaagtcaa ccagaatggg gttttttgaa agggttgcca gggtaagcgg ggtgatgaac
    14101 tccttggcat gaggggtcat aataacctta acctcagcac cttctttaat cagcagacgt
    14161 gtaagaatag ctgccttgta ggctgcaatg ctgccggtaa cgccaagaat tatatgtttc
    14221 cccgcaagac gcattactgg ttggtaactt cggtgttatc aaccattttg tagtaaagct
    14281 ttccatccat aaattcctga atggcaataa gggttggttt aggcagcttc tcgtagtact
    14341 ttgaaatttc tatttgctcc ctgttttcaa acacctcttc aaggttatcg gtgtagtagg
    14401 caaactcctc gagcttatgg tttagctcct gcttaatgtc aacgctcagc ttgttagccc
    14461 ttttggcaat aattctgacc gtttggtaaa tattcccggt ttccttatct agctcgtcaa
    14521 ggttgcgcgt gatggtcgaa gccggaacgt tcattttttt taaatccatg ggtttactat
    14581 tgattgttag ttgatttgca aaactttcat tgagctctca tacatctttt tagcctcggg
    14641 caagtagggt gattccggaa actcgccaac aaaggtatag tactggtcaa tggtattttg
    14701 gtagcgttcc ctttgcttat tgggaatgct gttttcggca agcaggtacg acgatttaac
    14761 cagcaggtag gatagctctt cacggtactt actgtttgga aaatccttca agctgttctg
    14821 aatggcgacg attgcagcct tataatcgcc aattttatag tatagcttag agctgaggta
    14881 ggacttttcc accaattttt ccctgagctc ggtgagcaac ccgttaacct cggtcctacg
    14941 gttggtatta ggaaagcggg tgagaaattc cacgaaagcc tcaattgctt cctgggtact
    15001 ggcctggtcg agttccggac ggggtgaaga tttatagaag cagtaggcat acatgaactc
    15061 ggcatcctcg gtaaaagcgc ttcgttgaaa tgtctgccta aacttgtcat agctatgacc
    15121 agcaagcagg tagtcgccct ggtagtagta gccttgtgca atgtagtatt ctatagtatc
    15181 cgatctaaag gttcccttgt agatgggcga tagctgctcc agcagcgtgg tgtagcggta
    15241 atgttctttt ttttcgtagt actccagtgc ttttttgtac ttatactcgt aatccttact
    15301 cttcaggagt ttttcataac cgctgcaaga aaccatgaaa atggtggcaa gaattgtaaa
    15361 aagaaataaa attcccttgg tatctctgtg ttttgtcatt atagggcttg tttaatgctg
    15421 cttactctct ttagctgaaa attaaacaaa ctgctatttg agcccttttt cgtaggctgt
    15481 tttgcagctt gcagtagcat aaaatagcag gcaaagttat aaaacttagc ggtaaattaa
    15541 aacttgaaac aagctgtaca tctaaaaaag gttaattacc cttgcagggt atcgaggatt
    15601 tttgggtaag aaaggttata tctttctttg atattttact gtgtgggtcg tagggcagct
    15661 acttttccga tcaaatgttt gaaaaacttg tttggtattc ttttgtaaga tcggtagttt
    15721 aggtgatgga accagctgat gcttacggtt tccctcaggc agaagtttgc tgttggtgag
    15781 cgctttctgt ttgtatgctg ctgataatct ttactctaca tacaagttag ctggctttat
    15841 aaatcttttt acgggatgtt tcaatttttt caatattgaa ataaaggacg tttttttaat
    15901 aaaattgagc tagatttgcg gctaaatttt aacactttat tcattccatc ttaactttta
    15961 aaatttacag cagtggatat ttttgataaa attgataaca tggggcccat tgggcaatgg
    16021 caaagccaga gccacgggta tttttctttc ccgaagttgg aaggagaaat tgaaccgcac
    16081 atgattttta ggggcaaaag ggtgctaaac tggagcctaa acaactacct ggggcttgca
    16141 aacctgccag aagttagaaa ggcagatgcc gaggctgctg cgcagtatgg gttggcttac
    16201 cccatgggtg cccggatgat gagtggtcaa acccgtaagc acgaggagct ggaggaggaa
    16261 cttgccaggt ttgtaggaaa gccctctgcc tacgtactca actatggtta tcaggggatg
    16321 gtttctatta tagatacgct gcttggcagg aacgatgtgg ttatttacga ttccgaatcc
    16381 catgcctgca ttattgacgg gcttagaata ttccccggca aacgttttgt ttaccagcat
    16441 aacgacatgg aaagctgccg taagcagctg gaacatgcta cagctgttgt tgagaaaaca
    16501 gggggcggca tcttgctagt tacagaaggc gtattcggga tggccggtga ccttggtaac
    16561 ctgaaggcta ttactgacct aaaaaaggag tttaactttc gcattctggt tgacgatgct
    16621 catggttttg gcactatggg tgcaactggg gcaggtactt ccgagcattt tggagtgcag
    16681 gatcaggttg atatctactt tgctaccttt gccaagagta tggccggaat aggagctttt
    16741 gttgcctcac aagagcacgt tatccgcttc ctggcctaca acatgcgctc tcaaattttt
    16801 gcaaaatcgc ttcctatggc aatggtgctg ggtattcaga agcggctcga aattttgaaa
    16861 tcccatcccg agcttaggga gaatctctgg aatattgcag gcgagctgca aaaaggactt
    16921 agggagatag gttttgacct tggccgtacc caatcgccgg ttacgccggt ttacatgttc
    16981 ggcggaatac ctgaggccgc caatatgatt ttcgaccttc gtgaaaactt cggcgttttc
    17041 tgctctatgg tagtttaccc tgtggttccc aaaggccaaa ttatgctaag gttgatacct
    17101 acagcagccc acacccttaa cgatgtaaag gaaactgttg aggcattcag ggtggtgcgc
    17161 gacaggttag ctgccggtaa gtataacaag gaaaaaattg cgcagtttta acttttagca
    17221 accatacgtt gaagcgaata cttgatgcta gccccgggag gactagacaa gtcgctgaaa
    17281 tgtgaaaggt tgcccgatca gaatacttaa ccctttgcac ctgaaatctg caaagggttt
    17341 tcttttttat atccaaaatc tgcccggagt ccctggagaa acgaatctgt cgaaggtcgg
    17401 acttgttccg tcagaaaccc gacagtttca ctcagagccc gacaagcttt ctttgatttt
    17461 agcttgcttt tgtctagaca acacgctacc caggctttct ttggctggta aattattcag
    17521 gtcggcaggc aagttgatgt tgtaacaagc gcctttagca gaaggggtag ggggcaaatc
    17581 catgtgattg gttacacaag cccgatgcaa ttctgatatg atatgatcaa aaccatccca
    17641 taaggtttct tcaaccatgg aaaggctccg tttttgagta aattccaaat caaggttcgg
    17701 caagcccgtt tatactaggt gcaaatatct ccgtcattgg cttgatgctg aaatgaccct
    17761 gtatgatttt ttaggcaaaa tttctatcgg ggcagggcgt atccgatgca gctataaata
    17821 caagttccgt gaaggagtgg aacagggcag aatgtaaacc gcctagagga cagcccttat
    17881 tcaacgtgtc ggggtaaata tttgacggag tccgatattc tgtacttgtg gtgtttgtga
    17941 ttttgattac ttgggtaaat aagctcgaaa ggctttcgta aattctttga attattggtt
    18001 tttctttcaa cttttaaaaa agccttacca actctaccca ttctggtagc tttgccactg
    18061 gcaagaatag ccagcgtgat ggaatgttgc atggtagggt taaaagacaa gttcgcccaa
    18121 cccttcacgg attatagctg gttcgtcgcc tgtacagtca acaaccgttg aaggaatatt
    18181 attacctgcg cctccgtcaa taaccaggtc aaccaaattc tggtagtttt caaaaattag
    18241 ctcgggatcg gtggtgtact caatcacctc gtcgctgtcc tttagcgatg tggtgaggat
    18301 ggggtttccc agctgacgga caatttcaac gggtatggga tggttgggaa tgcggatacc
    18361 cactgtttta cggcggctta ggaagtaatc gggaacccgg ttaagaccgg ggaggataaa
    18421 ggtaaatggg ccgggcaaat tctttttcat cagcctgaat gtgctgttat ccacccgggc
    18481 aaaatcggct atgtggctaa ggtcgtggca gataaatgaa aaattggcct ccttaggctt
    18541 cattcccttt atcttgatga tgcgctccac cgccttggtg ttggtgatgt cgcacccaat
    18601 gccgtaaacc gtatcggtgg gataaatgat gactccgccg cgcttcagca cctcaaccgc
    18661 acgctcaata tcgcgggggt tgaggttctg agggtacatt ttaatgtaga ggcactcggg
    18721 cattaaccgt taaccttttt aaagtcgtcg aggaacttct gaagtccgat gtcagtaagg
    18781 ggatggttga gtagcccaag aattgcattc aatgggcatg ttgcaacatc ggctcctgcc
    18841 tctaaacact ggataatgtg catggtatga cgaatggagg cagccagcac cttggtttgg
    18901 taaccgtagt gcctgtagat ttcaacaatt tgccggataa gcgcaacacc atcggtggaa
    18961 gtgtcgtcta acctaccaat gaagggagaa acataggttg ccccagcctt tgcagcaagt
    19021 agcgcctgtc ctgccgagaa aaccagcgtg cagtttgtac ggataccgct atcggagaaa
    19081 tgctttatcg ccttaatgcc ctcccgtatg cacggaacct tcacaacaat ctgagggtgg
    19141 agtgcagcta gctccttgcc ttctcttatc attccttcgt aatcggtggc aattacctcg
    19201 gcgctaacat caccctttac tatgtggcag atatcaacat agtgcttttt aatattctca
    19261 ctacccctga tgttctcctt ggccatcagc gaagggttgg tggtaactcc atcaagaacg
    19321 cccaaatcgt tagcttctcg aatttggtca agatttgccg tgtcaatgaa aaatttcatg
    19381 ctacctggat ttttagattt ctttgcaaat ctacatattt tttagtgggg cagcaactcc
    19441 taacatggtt gcaccagttg ggatgtttac atatttttgc taaaaatgtt aaaatgatga
    19501 agggttggtt tattcttacg attctacttc tcctcacttt tcccaaagtt tttgggcaac
    19561 aggttgctga cactcttaga tttaataatc aggaattgtc ggctaacaag ccgaatgata
    19621 tttcagtttc tactcaaatt ttcttaggtt accgctactt tatcggaaat agtgggagca
    19681 ctaatgaatt tactgtaaag cgaggctata tcaacttcat aaaatcgctt aacaagaata
    19741 tttcgggaag aataacccct gatattactc tcgatcgcga gggagatggt cagggtgata
    19801 tggaaatgcg gctcaagtac tgctatatga agctggaaga gccgggtgat ttctggattt
    19861 ttaccaatcc tgctgtattt ataggagagg tgttcactcc gtggattgag tttgaggaga
    19921 aaatcaaccc ctataggatt cagggtaacc attttctaga gaagtcagat attttgattt
    19981 cagccgattt tggggtgatg gcagctgcat tgctgggagg aacacttaat gaaggttacc
    20041 agacacgggt tggtagtgac tatcccggca ggtatggatc gttggccatt ggtgtgtata
    20101 atggcggcgg ttaccataac atagaggaaa acaataacaa gaccttacag taccggttaa
    20161 ccctacgtcc cttaccagac atgattcccg gtttacagct atcctataca ggggcaaccg
    20221 gaaagggaaa caccacgctt tctcccgatt ggaataccaa cgtgggtgtg ctgtcgtacc
    20281 agggccagcc cgttacactt tgtgggcagg tgtttagcag tgttggtaat tatagcggtt
    20341 ctttagccga cacccttggt gtttcctata aggtatgggg atggagtgct tttgttgagt
    20401 ttaagttgtt acgggatagg ctctccatct ttggccgtta cgattatgtt aagggtcgaa
    20461 atctctctga atttggaaac attacgcaga gcgtggtagg agttgcctat agtttattta
    20521 gacgcaataa acttgttgtt gattacagcc gtaatgaaag tagtggcatg gttgaggagg
    20581 taatcgaatt tatggtggag ctggcctttt aaaaagaaaa ggaggcaggt tatttgaacc
    20641 cgtctcctta actcttgttc atttagtatt tagataatta gaacaaccca gtaattactc
    20701 cgttgttatc cacatccatt cgttcggctg cgggttcatc gggcaacccg ggcatacgca
    20761 taatctggcc ggttatgggg ataataaatc ccgctccgga ggccacttca atttcccgca
    20821 cggttactac aaaatccttt ggacgaccca acaggtcggg gttgtccgat agcgattttt
    20881 gggtcttggc tacgcatatg ggtagcttat cgaggccaag ggagtaaacc ttttccaggt
    20941 cagcttttgc ctggggggtg tagtcaaccg cctcagcgcc gtatatcctc ttggccaggg
    21001 tttcaatttt taactcgggc gtccagctcc aatcgtaaag agttttgtgc tgagcagagc
    21061 agctcgatgc aattttgttc acaatttcgg ccagctcaat agctccttct ccaccttttc
    21121 cccaaacatc aacagcggct acttgaacgg acatatcacg gcacacctgt ttgatatact
    21181 caacctcctc atcggtatcg gaggtaaagc ggttgatggc taccaccggg catataccga
    21241 attgcttcat gttttctaca tgtttctgca ggttgccaat tcctcttttg agcgcctcca
    21301 aatcgggctg cgatacttcc ttcaacggtt tattgccatg gtactttaac gctcttacag
    21361 ttgcaaccag aaccacggtg ttggggttta agccggcata ctcgcacttg atatcgataa
    21421 acttttcggc gccaaggtca aacccaaatc cagcctcggt aactacaaag tcgctgagcg
    21481 ataggcccag cttggtggct agaaccgagt tggttccctg ggctatgttg gcgaatgggc
    21541 ctccatggat aatggccggg gtaccctcaa tagtttgaac caggtttggc atgatggcat
    21601 cgcgcagtag ggcagccatt gctccctggg cgtttaggtc gcgggcgtag atgggctttt
    21661 tgtcatatgt gaaaccaata aagatgttgc ccagcctttt tttcaggtcg tcgatattct
    21721 cggccaggca aagaatggcc ataacctccg atgctgcggt aatgtcaaat cccgtttcgc
    21781 gagggacgcc atgtgatgtt cctcccagcc caacgatgat gtgtcggagc gagcggtcgt
    21841 tcatgtccat aatccttttc caccttacgg ttcgggggtc tataccgagt gagcgttttt
    21901 tgctttggat gttgttgtca atcagggctg ccaacaggtt atgggcacgt tcgatggcgg
    21961 caaaatcgcc ggtgaagtgc aggttgatgt cctccatagg aagcacctgg gagtatccgc
    22021 caccggtggc accacccttt actccaaata ccgggcctaa tgagggctca cgaagaacca
    22081 ccgtagtctg ctttcccaac cggttaagcg cttgcgccag gcctattgaa accgttgttt
    22141 tcccttcacc tgctggggtg ggtgaaatgg ccgaaaccag caccagcttg tttttctggt
    22201 acttgttttt gtcgatatac tttagcggca ccttagcctt gtacttcccg taaagcacca
    22261 ggtcgtccgc atcaattccc agtttgcccg ctatttcggt aactggcttc attttaacct
    22321 catgggcaat ttcaatgtct gatttcatta taatagatat ttaaaatagt ttgtatttat
    22381 actttgtagg ttccaaaggg ttgaatatga aaatttaaaa gacaggtaag ttgaaaatta
    22441 aatgatgttg ttgcgctatt acttactgat attaatgatt agcaacaagc tgtttcattt
    22501 tttgtttatg ctttgatggt tttttggtcc attttgttgg tgccttcagc tttttaaaaa
    22561 tgcaattggg gttcatgcta atgctatttc tacttcgttt tgaaaatctt taaaagtgaa
    22621 atttgcaagt agcttactat ctaggtttat acctcgacat tgccagaaac ttttggtagt
    22681 cggatatgcc catcatttcg cttagggtaa gggtatcgtt atttttcatc aacgattcca
    22741 caattctgta gcctatccaa acggcagccc tgcctggcga ctcctgtgga aatcccgagg
    22801 taaagggtgc atcattaata aacttgttaa tatccagcgt gttggtgcta aaaagaagct
    22861 tattctcaat taaattagtc cacatcgctt tctcgttttt caggcaccag cggtgctggc
    22921 tgggtgtgaa accaaaaatc agcgaatcgg actcctgcgg tagcatttgc ttggtaaggt
    22981 acatcacctg tccctcatat attaggttgc ttagcaggtt gtcagtagag tcgtggaagc
    23041 tgaacgaacc atacatccag gcgcgaatgg catccgaagg tatcttttct ggacgcatgt
    23101 ttctcacctg gtagcgagga atacccagca gcttatactg aacggtattg ctgcccagat
    23161 atcggtcaag cccaatagcc agaatgctgt cgtcaacggc caacgaaagg ttgtaccccg
    23221 agacaaacgt gtaaattttt ggaactggct tatcggggaa gtagcagtga aatctattga
    23281 atgcttcatt aaggcagacc ttaatactgc ttatgttttg gaacttttgc tgcgataatc
    23341 ggtaggcgtt ttgcaccatg gtatcgctta gaaattgcga caggtaattg cagtactcgg
    23401 ggctgtcggt ttttccaata tggataattc cctcggagaa taaatcgaca aaacgcccgt
    23461 acttctttct taactcgggt actagggtct gcaggctgtc gggcgaagca gaaaataaat
    23521 cctgctcaaa cctctccagc tgaatgctag ctggaacatt ggtcgggtca atcctctcag
    23581 tcctgttggg tttgcaggaa atgaggagca caagagcagt aagtgaaaag agtatgaccc
    23641 ggttcatgca ggttgacatt tgcccccaaa gatagcaaag cgttatggaa caagagtatt
    23701 aaaattagct aagcatcaac caatatttta gtgtggttaa taacttgttt gtaagcctgt
    23761 ctcttttgtt aaaaaacact ttgccaccca ttggggttaa gagctgggct attcttacct
    23821 ttgtgggcta agaaaaatga tatggcagaa aagaacccta caggcagcag gaagaccagg
    23881 gtggctaaac ccctcgcggc agaaatgggc aaggtgcccc cgcaggcaat tgaacttgaa
    23941 gaagcagtgc tgggcgctat tatggtggaa aaggatgctg ttgtcatagt cagcgaaatt
    24001 cttaaacccg aatgcttcta taaggagacc caccaggaaa tttttaaggc catacaggat
    24061 ttatcatcca ggctggagcc catcgacctg tttacggtta ccgaggagct taggaggcag
    24121 aacaagttgg aggagattgg aggagccgct tttctggcac aacttaccac caaggtgggg
    24181 tcagcagccc atctggaata ccatgctaag attattgccc aaaagtttat tcagcgtgag
    24241 ctgatacggg tttcaaccga tattcaaaac aggtcgttcg atgaagatgt ggacgttgat
    24301 gagctgctcg attactccga aatggagctg tttaaagttg cagagggaaa cattaaaagc
    24361 gatacgcaac ccattaccac catcataaaa aaggcaatta agcagattga ggaagcgggg
    24421 aagagggaag acgggttaag cggtgtgcct tcgggcttca cagcgctcga caggcttaca
    24481 tcgggatggc agaattccga cctcattatc gtagccgccc gtccatccat gggtaaaact
    24541 gccttcgttc tttccatggc caggaacatg gcggttgacc acgacgttcc ggttgcgttc
    24601 ttctcgctcg agatgtcgaa cctgcagctg gtgaacaggc ttattgttag cgagtcggga
    24661 attgcgtccg aaaaaatccg caatggccgg atcgaccaaa gcgagtggac tcagcttacc
    24721 gttaaaatta aaaatctttc ggaagccaaa atctttattg acgatacacc cgcactttcc
    24781 cttttcgagt ttagggcaaa gtgccgacga ctcaaggcac agtatgatgt gggcgtgata
    24841 attattgact acctgcagct tatgaccggc ccttcagaaa ccaagggcaa tcgtgagcag
    24901 gaggtaagca ccatttcccg ctcattgaag gcaattgcca aggagctaaa cgttcccatt
    24961 attgcccttt cgcagctcaa ccgctcggtt gaaacacgag ggggatataa acgaccgcag
    25021 ctctccgatc ttagggagtc aggtgctatt gagcaggatg ccgacgtggt gattttcatt
    25081 caccggccgg agtactatgg ttttatggag gatgaggatg gaaattccac ccaggggatt
    25141 gccgaaatta ttgtggccaa gcatcgtaat ggccctgttg atgatattaa gcttaacttc
    25201 cgtaaggaga tggcgaaatt ttccgatttc gacgacgtgc actatggcga tattcagccc
    25261 tcgcattcac ctttcacctc gtcggtgccc gtaaccttca gctctaaaat gaacgaggat
    25321 acccacgggg attatgattt tctctccggg caaggggttg atagcagcga tattcctttt
    25381 tagttgaact agtagcgtat caagagagga tgtaggtgta gagtgttttt gaagattaat
    25441 tgatatgccc cagatttaca actctttata ttttcctgca aaagaaaatt tgcagctttt
    25501 tcccgcttta gagtatttgg gtgatttcct taagggtttc cacctcgtag gttacttttt
    25561 ccccgtgcgg tttcctttct gggttgaaga agatttggtc catcccaaac ttcctggccc
    25621 caactatatc tacctctaaa tcgtctccca ccataatgcc ctgatttttt ctgatatttg
    25681 cactggaaag tgcataaccg aaggcgcgtg ggtcgggttt atggtagccc gaaagttctg
    25741 aggttactac cctgtcgaag tagtgattca gcccacacct tttcagctta tcaacctgca
    25801 cttcatgaaa gccattgctc agaatcataa gcccgtactt tggcgcaagg taatcgagta
    25861 gctccttggc atttggaatt aggatggttt ttaagggtaa tatgcggaga tactcctcgc
    25921 caagtttggc tgcaagttgg tgattgttta ctcctaactc ggaaagtgtg gcatcaaatc
    25981 gtttgtatcg cagcacctcc ttggttagct cccctttgca gtagctattc cataggtagt
    26041 tgttgtgctt tacatacgtg cgggaaaatg ttgtggcatc ggcaactacc gagtcaagct
    26101 tgtattccga aaacagctca tgtagagctt cgaggctgtt tcgctcaaaa tcccaaagag
    26161 ttctatccaa atcaataaaa acgtacctgt acctattgct aatgctgaaa aatttaatcc
    26221 tcataacccg ctccttttcg ctgcaaaagt agtaatttac aaaattatag ctaccaggtt
    26281 acttccccac aattagccag aactaaaggc ataagaaaac ggagtagggc aatatttata
    26341 aatgaaataa ttcagaatgg tagcggttta cagagagtat ttaatgaata ttttgaccaa
    26401 aaaagggggg ggtgaaaatt tatgatagca tgaaatagcg cttctgcgcg tcttttaggt
    26461 tgaatattta acccagcacc aggcagggta gcagcacttt ttcacctaaa tttgaaaaca
    26521 gtatctttgc atccgaatac atcaacaaac attaaacttg aattttagcg agttaaacct
    26581 taatcctcac atacttgagg ggatttcgac aatggggtat ggaaatccta ccccaataca
    26641 ggagcaggca attccggtaa ttcttcaggg gcgtgattta atagcctgtg cacaaaccgg
    26701 aactgggaaa acagctgctt ttttgctgcc tattctgggt aaaatagccg aaaagcctac
    26761 cactgaaggc tacataaaca ccctgataat tgttcctacg cgtgaacttg ccctgcagat
    26821 tgaccagcag gtagagggtt ttgcctactt catttcaacc agctccattg ccgtttacgg
    26881 gggtaatgat ggcaacagct gggatcggca gcgcatggcc ctggagcagg gggctgatat
    26941 tatagttgca actcccggaa ggctcctcca gcatattcaa atgggctacg ttaagctgga
    27001 taggctggag catttaattc ttgacgaggc cgaccgcatg ctcgacatgg gtttttttga
    27061 cgatatcacg gcgattatct cccagctgcc aacccgaagg caaaccttga tgttctctgc
    27121 aaccatgcct cctaaaatcc gccagctggc caagactatc cttgacaatc cggttgaaat
    27181 aagcctttcg gtctctaaac ctgtcgaaag tattttgcag gcggtttaca tggtggagga
    27241 ttcagaaaaa atcggattgc tacagtcgtt ggtggccggt aaggagtacg tgcaaagcgt
    27301 tctggttttt gcttctaaaa aaatggaagt aaaggacatt gagcgcaagc tggtaaggga
    27361 aagcataatg gcaagggcaa ttcattccga ccttgagcaa agccagcgtg aacaggtgct
    27421 tcgggatttc cggaatcgaa aatttcaggt gctggtggct accgatattg ttgcgcgtgg
    27481 aattgatatt gaggacatag acctggtggt taactacaac gttcctgccg atcccgaaga
    27541 ctacgttcat cggattggcc ggactgcgcg tgcccaaagc gacggcgtgg ccatcacttt
    27601 cgtcaacaaa aaggagagac cccagcttgc ccgtatcgaa aaatttctcg gcgagacaat
    27661 tttccgggtt agccactcat aagcgcttac tgttaggatt attaaatttt gattgatcca
    27721 gctttagtct caatgggggg cattgcccgc catttggcca catgccgcat caatatcccc
    27781 tcccattgat ttgcgcaggt ttgtcgggat acctgcatcg ttgaggagct ccataaagtt
    27841 agtaagttcg tcgctggtgg gtgattggaa ggttgctgca ttgtgccggt tccatgggat
    27901 aatgttgaga tggcagctta gtccacttag caatgttgct attgccctgg cgtgattttt
    27961 agatagattt aggctgccta acgcgacata ctcaaaggtg agccttagcg gtttttttag
    28021 ggggtagtgt ttgagcagct tgattatttc aagtattgga tttgttcttt ctacgggcat
    28081 aagcattgcc ctttcatcgg gaaaaggtga atgcaggcta attgccaggt tacagccggg
    28141 cgattttaga aaggcctcca gttcggaaag caaaccaact gttgaaatgg taatattcga
    28201 tttacctaag gcgcaacccc aatcggctgt caggatttca agcgttttgt ttacaacttt
    28261 caaattatca agcggttcgc ccatacccat cagcacaatt ctgttcaacc ttttgctatc
    28321 tggtatgcta agcacctggt tgatgatgtc gcctgctgat aggttgccat taaagcggat
    28381 gcttcctgtg cgacaaaatc tgcatcccat tctacacccc gattgggttg acaggcatag
    28441 ggtatgccga ttgcctgaac tcatggctac agcttcgaaa acctggtttt gcccgttggc
    28501 aaacaggtac ttcacagagc catcgtcgct ccgtttactt tcttttggag ggtatagacc
    28561 taccgtatag gactgttcaa ctgattgcag aatagctttt ggtatatcgg agaaggtaga
    28621 aatatccttt tcgcgtttac ggtatatcca gcgcatgagg ctttcagcct gaaagctagt
    28681 tccaccatat tcctgcatgg tgctgcaaag ctcctgtgga gtgaaaccta tgagcggaac
    28741 cttgttattt tgtttttcca tcaacttcaa ttagcacagg tttactgaaa ggcatatcaa
    28801 catatcaacc aatgctgtaa gcgctattct gattattggg cctccatgta aaattgaata
    28861 cttggcaatt ctttttacct ttgtatccac gaaagcatca atccaaatga gcaatcggac
    28921 aggagcacat tctgcatatc aaaagagcat tcttttcatt cttgcttttg tgatattgct
    28981 ccccctattc gggtgtagca acagaacggt tgttcaaaaa tcgggaaagg ctaagtacac
    29041 caaaaagtat aagtgcaaat gtagtaagcc taaggcgaac cgtcaaacct acaagtatct
    29101 gaaatgagta caaatgctga gattgagaaa aaaccttcta ttgccgtagt tggaagcgga
    29161 agctgggcaa ccgcactggc aaaggtgcta catgaaacac aggactcaat tggctggtgc
    29221 cttagggatc cggagttaat tggctatatt aaaaagtaca ggcataaccc caactacctt
    29281 agtgcggttg agtttgacgt taaaaagttg tatttaagcg tcgatatcaa cgaggttgca
    29341 aaattatacg acattttact tttcgttatt ccatccacct actttcaagc ctggctggaa
    29401 ccccttagtg ttagtttaga aggtaagttt attatctcgg caatcaaggg tattgttccc
    29461 ggaatggatt gcaccattgc cgagtttttt aaccagcagt atggcgttcc ttttgaccga
    29521 attggaattg ttagtgggcc tacccatgca gaagaggtag ctctggagag gctttcatac
    29581 ctcacaattt cttgcaagga caagcttcag gctaagcatg ttgcgtccta ctttgaaaca
    29641 cactacatca aaactaatat tggaacggat atttatggca cagagtatag cgctgtgctt
    29701 aagaatattt atgcagttgc ctcaggaatt gcccacgggc ttaactatgg cgacaacttt
    29761 ttggccgtgc ttatcagcaa tgcccaacgt gaaattgagc gatttcttaa gaaaacatat
    29821 cccagtaagc gaaggctaag tacttcggtc tacctgggcg atttgctggt tacctgctac
    29881 tcgcagttta gccggaaccg cactttcggc accatgattg ggaagggcta cagcgttaaa
    29941 tcggccatgc tggagatgag catggtggcc gaggggtata atgcagctaa gtgcatttgg
    30001 gagattaata aaaagtataa tgttaaaatg cccatagcca acgccgttta caacattctt
    30061 tacgagaata tctcgcctgc tattgagatg aagctgttga cagcaaagct aaggtagatg
    30121 aaattttaac ccgttaattt ataaaaatga atcagttgaa agttgactta tcaaacattt
    30181 atacccaggt agcaaaggag gaagtctttg cccttaaaac caaaatagcg caaaatcagc
    30241 tgctccttga gcagggtacc ggtaaaggta gtgattttct gggttggctc aacttaccct
    30301 caagtattac taaggaacaa ctttacgata tacaacatac agccagtgag cttcgggcta
    30361 aaagtgatgt agtggtgata gttgggattg ggggttcata cctgggggca aaggccgcta
    30421 ttgattcact aagtagcagc tttacccagc ttaccgagga cgttttgcct aaaataattt
    30481 ttgcaggaaa cagcatttgt gaggattacc atgccgaact gctcgaactc ctcgaaacta
    30541 agcgctactc aattgttgtt atctccaaat cgggcacaac cactgaacct gccataacct
    30601 ttagggtgct gaagcagcat cttgagagac aggttgggaa aaccgaggct gcaagtcgta
    30661 ttgtggctat aactgacctg tcgaaaggag cgctgcgtaa gctggccgat agcgagggat
    30721 accgtacttt tgtcattccc gatgatgtgg gtggaaggtt ttcggtttta acacccgttg
    30781 gtttactccc cattgctatt gccggaattg atattgtgaa gctggtggaa ggggccagaa
    30841 aagccatgga cgacacctct tcatctgtag cttttgacca gaaccccgca tgcatatatg
    30901 ctgcagcccg caatgccctt taccaaaagg gcaagaaggt ggaaattttg gttaacttca
    30961 ccccacgcct tcactacctt accgagtggt ggaagcagct ttttggcgaa agcgagggaa
    31021 aggaaggtaa gggaattttc cccgccggcg ttgacttcac caccgacctt cactccatgg
    31081 ggcagtacat ccaggaagga gaacgtatgc ttttcgaaac tgttctttcg gtcgaaaaac
    31141 cctcccaccg ccttgttgtc ccagacgatc ccgataacct cgatcagcta aacttcctct
    31201 cgggtaaacg cttctcggag gtcaacctga tggcccaaat gggaaccatt gctgcccata
    31261 gcgatggcgg tgtaccctgc atttctatca gtattcccga actttcaccc tactgggtgg
    31321 gttacttgtt ctactttttt gaaagggcat gtgcgctgag cggctacacg cttggggtta
    31381 accccttcga ccaacccgga gtggaggcct ataaacgcaa catgtttgcc ctgctgggta
    31441 agcccggcta cgaggctgat ggtaggaagc taagggagaa actagggcta tagctgcttg
    31501 ttattcggct aaggttctcc ggctgaattt gatgagttgc tccagtctgt agctagttgg
    31561 taaaagttca tggtataagc ttcgttctta ccctaaattt aaggtagaat tttttgaaga
    31621 ttgccctgca tacgatggta atattatagc tgcatattca aattaaaaaa aagcaggagc
    31681 gtgtttctcc tgcttttttt ggttgtatca gatcaaggaa ttgaccctat ctggctacca
    31741 ctatcttttg cgtttcaaca gtgtgatgag tgccggaatc gtaggttacg gtaagcaggt
    31801 atattccatt tgatagctca ttaccactta gcggtaagga ctgcaccccg gcctcatagt
    31861 gggtgggcat gctaccgcca acccgctgcc caagcatgtt gtagaggtca actgctaccc
    31921 taccagccat tgggagggtt atgcttaccc gtgagctggc atttgcagga tttggaaaca
    31981 ccttgaaatt taccgcattg gctgtcaggg tttctgcatc gttggtttca gaccccacca
    32041 cagcccttac cagaaagttg tagttgagct gggaatttat gttgataagt tcataccatt
    32101 gattgttaat gtaaatccat cctcctttgc ctgctaccat tgggccgcca tcggtggcaa
    32161 aggggtagcc tcctgacgct gttgcggtat atcccaccca gtactccttg ttgggctgta
    32221 tcacgatggg ctgtgagagg gtgtgcgacg tccactggtc gggaacaacc tctgaggata
    32281 tattttttga gtagataagg gagcccgggt taccataggt tcccccttcc cataccttaa
    32341 ccgtttcgtt ggtggttaca tcgcttccat tctctcctcc cctgatgtag aaatccacca
    32401 tctttatttt tctgcctacc agcgatgcca aatcttgagt ggtaaagcga gtggcaacgg
    32461 tcagtgtagt gctttcatct ccaattgctg tggcatagcc cctgtcattt tttagcacta
    32521 cgtaggggat aaagtctatt gttgcatcaa acgagatggt ttcaccggcg gcagtaatgt
    32581 tggaaatttt caggccaccg ttcgttccat cggtgagaaa actgctgggg ttcgtgttgt
    32641 cgtttatgct tgttctcccc acggacgagt tgaagtgtgc gctgttaatg gaaccgttta
    32701 aagtgggggt tccgtttggc cggtatatgt aaacttcatc gggaggccca tcggcatttc
    32761 caactccttc aagcttcgag tttattcggt acaccagaag tccgttgccc ggtaaatcac
    32821 tgtcgtagga ttcctttttc ctgtactcca ctacaaagta ctcactgtag ctgttgggcg
    32881 aggggatttt gtagctgttg ccggttggag atgtggccaa aggtgaaagc gtgtaggtgc
    32941 cggaagtagt aatttcgggt atttggtcaa tccaaccccc gtaaaggtac ttcatgtggg
    33001 cgcccatgtt ctgggggtag tccagggttc cctccatgat atcccaatag ccgacaggac
    33061 taaggtttag atcatcgtcg tagtggtata ggtcgggtgc gccaactgca tggaacattt
    33121 cgtggcagag ggtacccagg tcaatttgtg aacctccgaa gtactgcagc tgaagggtgt
    33181 aatcgccaac ctgtttaccg ttaatgaatg cactctgcga ccagaggctc cactggtggg
    33241 gccacagcag gtcgttccac gagtcattgc taccggctaa aatgaacgaa attacgtcaa
    33301 tgtagttgtc gccatttata tcaaggttaa ttccggttgg aatgcttgcc ccaacagcct
    33361 ccaccgcttt tttcagcagg gtgtgctccc ggtaggttga actcgttgga tcgttgtagt
    33421 tcgaagcgtc ggggttgtag ccatttgggt ttgtggtggc atcgtagggt tggtagtagt
    33481 tccggttgtt ctcgtcaaca taccaggtta caagggtgcc gttgagctgg gggtaaaagg
    33541 tggagctaac actgaactta ccgtacgaaa cagtagagta gtagttggct accgatgctg
    33601 acgaaccgtt gtaaacattt tcgtagtagg aaatattctc atcggctaca acatcatctt
    33661 taaaacggat aaaaaccaca atgttgttca tggtaacacc cttaaccgct ctaagtgggg
    33721 caataccgct agtccctttt gtaatatcaa ggtttacatg atggttgtcg gtttgcttgc
    33781 ggtggcgttt agccaggtac tcattacgcg aaatattcac gtagggcttt aaatcctttg
    33841 agtttcgagg atttactttt cctacaacat agtctgatga cgtcagcttc ccattctttt
    33901 caacagcata aacgtagaac ccgtttgcag gattacgcat gatggtgtag cctgcagcgt
    33961 cgtgtagcca gttatgaaac tcatcgccgc tgacaaagca gctgattttt gtgccgtcgg
    34021 gctgggtgat ggttataggt aaattatgct taggaacggc ataccctgta agggtcatta
    34081 ctccaataat ggccgtaaat gccaacttcc taatactttg taagtgtttt atcatgatat
    34141 taagcaattt tattgttaat ttagatataa aacttccagt tttaagtgta aactagtgca
    34201 ttggaatacc tttatttttt taagatattc ctcgacagga atccgtgaaa gcgtattgtg
    34261 ctggtttgca tagttttaat aacgggtaag ccctgtagct ttagggtgtc ggttttctcg
    34321 gtatagtgtt tccactcctc cccggcttta agggtaattg atttgttttg ataggttagg
    34381 aaaagcttgt tgttcgacag gctggttatt gtcaggtcgt tgtaagaaaa ggggagttgg
    34441 ttagccccta cgaggaatga ggaaaccccg cttccggcag cattctttaa gattttgccc
    34501 acaccaagaa caactatagc ggaatcgggt tgcagcccgt cggggtaacg gtatatgtta
    34561 agcgttctgt cctcggggtt gaatgcgtag gttggcgaat caattcgcct gccgccatgg
    34621 tactcacctg ccattacctg aacatctgtt gtttggtagt actcaacaaa ggcaaaagca
    34681 ttgttggtta cggttgaata atcggctgtt tttttaccgg aatagcatgc ggtagttaca
    34741 aataataaaa aagaaagagc aatagaaagt gctgccttat tacgattgtg tgccatagcg
    34801 aattgcttgt aggtgttttg caaaacccat ctgccttatg ctggtaaggt catactttcc
    34861 atgaatgtgt caattcatgc tagagtgaaa actctatgag gtttggctct tgtcgccact
    34921 aatccccatt ttatttgcag tacgttcagg aatgtagata accaggcgct gtccaatgcg
    34981 aatgatgttg cccctaaggt tgttccatga ccttagctga cccacggata caccgtattt
    35041 ttcggcaatt atccctaata catcgccttc ttttaccctg taaacatacc ggattgagcc
    35101 cgatggtata tcgtactggg aggatgatgt atatttattt gggctaatgg ttacccgtgg
    35161 gttaaaaaac aggctgtcct tataggcata gatatctttt tctctgtcaa taaaaggtcc
    35221 tactccgtcg agcggcagcc taagggcgta aggcttgaac ttgccgggaa taacgtcgag
    35281 cttgtactgg gggttaaggt cgcggagcat atccaaaggg atatccagca cctcggaaac
    35341 ctgttggagg tgaagcatgt cgttaatcac aatggtatcg gtagtagggg gtatgttaat
    35401 aggctgggga accaggttat gctcgtggct aaaatagtag gtgtagctgg cggctataaa
    35461 ggccgggacg tagccgcgtg tttctcttgg taggtagtag tatatgtccc agtagttgcg
    35521 cttgccgccc gagcgccgaa ttgccttatt cacgttgcct ggaccacagt tgtaggcggc
    35581 aatcaccaat gtccagtctc cataaatggc gtagagatct ctcagaaatc gtgctgcagc
    35641 gtgcgttgag gcaattgggt cacgacgctc gtcaacgtaa gagttaacgg ttaggttata
    35701 ccttcgacct gttccataca tgaactgcca caatcctgtg gcgccggcac gagaaacagc
    35761 tttggggttt aacgccgatt ctattaccgg taaaaacctt agctcctgtg gcagctggta
    35821 cttatcgagt atctcctcaa atatggggaa gtagtagtcg gttagtccta gcatcacctc
    35881 aacctggctt ttcttcttct gggtgtacac gttgatgtag ttcctgacta tgctgttgaa
    35941 tggcagctca ataagcgagt tgatggattg cagcctttta atgtagaatg agtcggggta
    36001 accggttaat cccacgctgt cgtcttctat gttggctacc aagctattgc tatctactgt
    36061 ttgctgaaca taccaaaggt ttaaaaggct gtcgaggttt ccgtttatgt actggatcag
    36121 cagggtatct tcgggtataa tatcggtgtc agggttgagg gtatcctgtg caaatgtagc
    36181 agtagtaaat aatagtagca gggttgcggt taggtaaaaa tgtttcatcc tttttattgt
    36241 tagtgtttat ccaacattcg cctagcaaat atagtaatga ttttttgtta cgttgacccg
    36301 ttgcggtcaa aaaaatcgga tgtaacccgg taataaatgt taataccctt gttaatacct
    36361 atatttcaaa ttgtttagcc ttgtatttct ttacatggat taggctaaaa agggttttag
    36421 gtcatccagc cagcggctga tggtcaaaag tgtacaggta atgtggttag ttctccaaaa
    36481 tttttagctc ggtgcgcctg ttcattgccc gaccttcggg agagctgttg ggtgcaacgg
    36541 gttgggattc tccataacct ttccagctaa ccctttcggg cgaaaccgat ttcgacagca
    36601 ggtagtccac aaccgactta gcccgccgtt ccgacagctt ttgattgtaa tccggtgccc
    36661 cctgattatc ggtatggcca ccaatttcaa ctttaatggt ggggttttgc gatagggttt
    36721 ccaccactct gtccagctca acagttgact cgggcaacag ctcgtaggaa tcggtttcaa
    36781 aaaaaacatt gcgcatgatg gtaacctcgc cttgacgaac cggttttagc agcacatcca
    36841 gatggtaggg tttatcgaag gagtagagtc ctttcaggtt aaagttttcc gagtaaaaca
    36901 ggtatccagg tgcttgggca aaaagcgcat agctgttgtc cgaagggagg caaaccataa
    36961 acccacctgt taccgatgaa taggagttca tggtttccct tttgctctgc aaatctataa
    37021 gtgaaatatg tgcttcaacc ggtttgagcg tggtgtagtc cctaattgtc ccggttacgt
    37081 aggatgatgg aatgggttga atttcggggt agagttcaaa tgtgtaaatg tccattcctt
    37141 tatccggtat gtagttggag gagtagtagg cagttttgcc aatggcgttt acaaccagac
    37201 cttcctcatt tttgcaagtg tttatagggt aacccaggtt gaggggttcc gaccatttcc
    37261 cagaatcggt aaggtgtgaa acgaagatat cctgaccacc cattcctata tggtcgtttg
    37321 aggaaaagta tagggtttta ttgtcaaaat gtataaaggg tgactgctcg ttgccgggtg
    37381 agtttaccgg ttgtccaagg tttacagcat tgccccacac ttcatttggg ttcttcaccg
    37441 atacccagat gtcaaatcca ccaagtccgc ccggccggtt gcttacaaag tacagcattt
    37501 gtccgtcggg tgagatggat ggttgttttt ccgatgattt ttcggagttt attgcgggag
    37561 gaagcttcaa tgggtgtccc caacttccgt cagagtttat ggtgctgaag tacaggtcgc
    37621 agtcgccttg gcaaatggtg tagtacatgg ttttaccatc agatgagagc gattgggctc
    37681 cttcgttaaa aaccgtgctt agcggggagc caaccggtat tcccttgctc caggttccat
    37741 ccttgtttcg tgtgctggtg taaaagtctt cctgccattt ggttcccttt attcctacct
    37801 ctggttcctt tggtaagcgt acggtgtaaa ctatggttcg ctcgtcaacc gataggctgg
    37861 gccagtactc gtcgagagtg gtatttatgc tatcccctat gttggctggg ttgaagggaa
    37921 cagggttggc aacagcatgg attgcaaacc tgcatctttc gatattattt ctggcaagtt
    37981 ccaccatgct gccggatgtt tcatggtagg ttaaatactt ttcgaagtac tccagggctt
    38041 tttggtagtt tccaatgctg aagtttagaa cacccaagtt gaagtatgtc ttggggaaaa
    38101 aattgttgtt tatgcttacc gccttttctg ctgaagaaat agctttctca ggttctccct
    38161 tttcagagta aacctgcgtt agcacaaggt aagcttcaat aaaattatgc tctattttga
    38221 tactctcctt cagcaacctt tccgcttcgt ccaggttgta gcggttatat gcagcaagtg
    38281 cttcgttgta gcgtgccacc gcccgcttat ttttagtgct cagattttta ggtgattgag
    38341 cactaagaag catgggtagc agaaatatca ggagcaggag tatttttttc atgctttgga
    38401 gatgtccagt agtgtgccag cagctaaatt ctacgctaca atatacaaaa gtaaacgaaa
    38461 gtaggtaaaa tgtatgattt gggttatttt ttatggatac tccttgctat taacaaccgc
    38521 ttggtttttg tagcagtgag ttaaagagcg aattttattc agcttacgga atgtgcatga
    38581 acttatgcgc ctgcagcgat attctccatt ttgggttttg ctttatgtag tccaccagca
    38641 agggggtaat gctttcgtgg acgctccatt cgggttggag aaaaagcatg cagtctggtc
    38701 gaaccctttg ggcatttgct tctgcccagc acaggtcggc ctgtttggca ataatcacct
    38761 tgagttcgtt ggcctttgaa tagtttaaat ccactggtgg ctgctgctgt ttaggagata
    38821 ggcaaaccca atcccattga cctgtaagcg ggtaagcgcc tgaagtttca aggaatgttt
    38881 ggatgttatg ttttttaagc tcgctgcaaa ggtacccgag agggtaaagt gttggttcgc
    38941 cacccgttac caccactgcc ctggcaggat atgctattgc cctttgaacg atctcgtcca
    39001 cgctaaccgg agggaatagt ctggagttcc acgattgctt cgaatcgcac cacgaacagc
    39061 ccacgtcgca tccaccaatt cgaataaagt aagccgccct ccctgtttga aagccctcac
    39121 cctgaagggt gtagaactcc tccataaggg gcaatacttt acccgactcg agttcaggat
    39181 ttatggttgt atgatcaccc atgctaatcg ttgcctgtgt atgaaaagag caacttgtgg
    39241 tcacgtgcag ctccgctggc agcccactcc tcaggaataa ttttccaagt accgaatgat
    39301 tttggggtga taactttttg ttgctcaatg aacttcatga tgtaaaagcg caggtcctta
    39361 tcggttgatg caacaatacg tttctgaata tcctcggccg agagtccgca gcccttagtc
    39421 aaatgtccac ctccaccatt tccacggtat gagttaatcg ccacccggta ggttttggct
    39481 gtatcgaacg gttctccatt gaccatagaa agtatggtaa ccttattgcc atcgggcttc
    39541 gatacatcaa ctgtgtaaac aattcctgct gctgaatcga agttgtagta gcggctagct
    39601 agctcaactt tccccctgtt atccgttgcc atgctaccat cggcattttg cctgaaccgt
    39661 aacaggtggt cgttggcgtc cttcatggtg ttgaaccata gtgatgcaga gtactccatg
    39721 taggcatcaa cctcggcacc ggtgagattc atggtgtata gaaaattttc aaaccggtaa
    39781 agtttaaaca tgtcgctaac cctcacaatc cctttgttta tggttgaaac aaacgataac
    39841 ggcgcagcaa acgaaatatc ggcattcgtg atgctgagct gggcgctgtg aataaaatcc
    39901 atgaaagcgg ttggtccaaa gtatgcattt gcgctggaga tggtttgtgt aaattctccc
    39961 aggggctggt taacaaagga gtcaaccttt tggtagcaac ttttaaattc tgccatgaat
    40021 gcggagtcgg gagaaaacct tcgggtttca accagtgttc cggtaatatt ttttgaaact
    40081 accctctttt tcttgtctaa tgaaagctgt atggttgcct ccgagatgta gcgtgctgat
    40141 gaggttgggt cgagaaccaa cacggaatcg ccctgcacgt tttcaacttt ggttatcagc
    40201 ttatcatggt cgtgcccaat gaagatgaca tcaaatcccg gtacctttct ggctaccagc
    40261 agggatgcat tctcattcaa cggagtttcg gcatttgcac ctccatagct ggagttatag
    40321 cccgagtgga agagtccaac cagcacatcg ggtttctcct tttcctgtat ttgttgaacc
    40381 cagtatgtag ccgattcaac catatcgtcg aaacgcatgt taggccaaag atttcgggga
    40441 agccagctgg gaatgccggg ggttattaac cccaaaatgg ccacttttaa accgcttcgt
    40501 ttaatgatgg tgtagggttt aaatgcaggt ttccctgtaa tggtgtttat tgcatttgct
    40561 gccatccacg gaatggcaag ttccctttct acttttcggt aaaccagcgg gccagtttca
    40621 atatcgtggt tgccaacggt tgccgcatcg tagcccatgt agctgaatac cctcgaaaca
    40681 atgttacaac ttgagctgtc aatgtagttg gaatagtaaa cggttggttg tccctgaaga
    40741 atatcgccat tatccagcag gataacctgc tgttgactat tttccctttc cttcttaacg
    40801 taggtgtaaa cctgtgccat gctcgattcc attggcttat tcccgataaa gtcgtatggg
    40861 aaaatagctc catgaacgtc actggtttcc agtatcttaa tggtgactgt ttcctgagcg
    40921 tagcagaata gcgacaaact tactaacgcc tgtagtagaa taattcttga tgtaatagat
    40981 ttcatagtcc ttaactttaa aatcgacggc gtaaaaatag cctaattttt cctttttgtc
    41041 tggcctgctt ggctggttaa ttttaagaat gagtaatggt tgtctggtct ttatgtgttt
    41101 attgatatgg tataatgcct gatttttgtt atgatgagtt ttcatcggtt ttactggtga
    41161 tatccggcaa agcttggtac cattcaggtt atttaggtct ttaaccctaa caaaatttta
    41221 ggttttctat cacaagtaat tttacctata ccctttaggc ttcggggttg ggcggagaaa
    41281 cccatatatt ggtttcacct gactcccatt acgcatggtt gtttatgggt gcttacgctt
    41341 gcccccctac acgggtgttt catatttttc ccatcttctt actgttagta gcatagtccc
    41401 ttatgttgag aaagattaaa aaaaagcttt aaaattttaa actaaatgaa acgatgaatt
    41461 gttatttgaa taacacaaag aggctataca gaaaaaaatg tgctaaagca accaaattaa
    41521 tgtaaaccaa aataaggagg aacaattatg aacacttacg atttgaaagg gaaggtagcc
    41581 gttgtaaccg gtgccatgcg cggaattggg aagggtatag ccctgctgtt agcccgaaat
    41641 ggcgcaaggg ttgtggtaac cgacattgat aaagctagct gcgagcaggt ggtaaaggaa
    41701 attgagaagg caggcggaac gggcgtggcc tttaagctag acgttaccaa cgagcaggag
    41761 attcaggatg tggtgaaatc cgttcatgcc cagtttggaa gaattgatat tctggtaaat
    41821 aatgccggaa tatttatcca ggaagagctc gatggtatgg atactgctaa aattgacaag
    41881 gaacttggcg taaacctacg ggggacgctt ctttgtacaa aaagtgtgct gcccataatg
    41941 aagcagcagc actacggcaa aattgttaac cttgccagca tagccggatt tgttggcttt
    42001 gaggtggcaa gcgtttactc agcaaccaag ggagccatag ttaacctaac ccgggagttg
    42061 gcgctcgagc ttggaaaata cggaattaac gtaaatgctg ttgcccctgg agttatagaa
    42121 acccctatga ccgatggact cttggaagat aaaaatgttg cggctgctct tctctccaaa
    42181 attccttatg gtcgtaccgg caaacccgaa gacattgcaa acggtgtagg ttttctggtt
    42241 agcgacgaca gcagctatat taccgggcat actctggtta ttgacggtgg ttggcttacc
    42301 atgtaatttt ttcacgaaat tgtttttgaa gccgaatttg taaattcggc ttcttttgta
    42361 tggtgtgtcg ggcaaacgct actacactac actaccggca agatgcaagt tc
//
LOCUS       DAOSHM010000018        41602 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_721_length_41602_cov_20.391196, whole genome shotgun sequence.
ACCESSION   DAOSHM010000018 DAOSHM010000000
VERSION     DAOSHM010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 41602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 41602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41602
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_721_length_41602_cov_20.391196"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="PK839_04540"
     CDS             complement(<1..406)
                     /locus_tag="PK839_04540"
                     /inference="COORDINATES: protein motif:HMM:NF024886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase N-terminal SAM-like
                     domain-containing protein"
                     /protein_id="HOG72039.1"
                     /translation="MREALESMAVIDVCSLSESVEAIQKSKGAVWCSIPDELNKFNAW
                     LLYRRYSTNTTRTYLETLRSFLIFLQPKAATDATNNDMVRFVNDYILKNGYSLSYQNQ
                     VVNACKLYFKEIAHGELERKLPPNSQYQGDLAL"
     gene            complement(417..590)
                     /locus_tag="PK839_04545"
     CDS             complement(417..590)
                     /locus_tag="PK839_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72040.1"
                     /translation="MTTELKVITCRQGTHRNNKVVFLNFEFDKTLIDAVRKLGCAKWS
                     QTDHKWYIPYREF"
     gene            917..1516
                     /locus_tag="PK839_04550"
     CDS             917..1516
                     /locus_tag="PK839_04550"
                     /inference="COORDINATES: protein motif:HMM:NF014659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HOG72041.1"
                     /translation="MAKFKPSEMDFFKFDFRQIPPKAGRLLIAQPSLTDPFFKRTVIY
                     LVEHDDEGSMGFILNRKLAMSLNDLLNNFPNVEANVSVGGPVGSDTVNFIHTFGSTIP
                     NTLDLGNGLYLNGDIDAVKALAIAGRLTRENFRIFIGYSGWGKDQLKREIDEDSWVVA
                     NFDSQLMAKGAYDSWYFAVQQLGSRFKAWTLYPENPALN"
     gene            complement(1489..2370)
                     /locus_tag="PK839_04555"
     CDS             complement(1489..2370)
                     /locus_tag="PK839_04555"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HOG72042.1"
                     /translation="MASRNYIWLNGQFFDGGNPVINFENRAFRYGDGVFETIHAFGTQ
                     PRHLPLHFRRLLQGMKILELVAPTWLTEQNLELAITKLLNKNRLFDSARVRVTVYRDD
                     GGFYIPSTSNASVLIDTQPLAAKMYTLNQQGCIVGIYDEIKKPLNTLSGVKSCNALLF
                     VKAGLYCMANNLDDCIILNEQGRVAEALSSNVFVVRGGKLYTPSLAEGCIPGIMRELV
                     CTVAPTVGIEVNNQVAINPSAFDSCDEVFLTNAISGIKWVVGIGTRRYFNRVSRTLSE
                     ALNRFTFPDQFRAGFSG"
     gene            complement(2377..2805)
                     /locus_tag="PK839_04560"
     CDS             complement(2377..2805)
                     /locus_tag="PK839_04560"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOG72043.1"
                     /translation="MEPLVVKICEGWLQSGEHRLVAIMARYRGGWIWVMNRNRSTWEL
                     PGGHIETGEEPDETASRELFEETGALDFSMVPLFDYTIQQGSARSDCRMFYAAVTKLG
                     ALPQSEIQKIGMFDSPPDELTYEGVQLQLFEAALGYVSES"
     gene            complement(2820..3437)
                     /locus_tag="PK839_04565"
     CDS             complement(2820..3437)
                     /locus_tag="PK839_04565"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008156828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOG72044.1"
                     /translation="MTYAIIIITSLVSAVALRNGELMQRLLLSPYLVFRNRQWYRLIT
                     HALVHANYLHLIVNMIVLLSFGKAVESILRQLNMEGVIGHPALHYLMLYLGGAVVATL
                     TTLKKFRNDYSYQSVGASGAVSGMIFFCIFFYPLNKLYLMGILPIPGILFAVAYLIYS
                     NYMSRKGGDNINHDAHFVGAVFGFIYPLLISPKLITVFLKQLGLM"
     gene            complement(3439..4476)
                     /gene="holA"
                     /locus_tag="PK839_04570"
     CDS             complement(3439..4476)
                     /gene="holA"
                     /locus_tag="PK839_04570"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007569639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HOG72045.1"
                     /translation="MAKSTTLDSYTKILEEFKNKIYKPIYLLSGEEHYYIDKLSDYLA
                     DNVLSATEKDFNLTIVYGRDVTIADVINTARRFPMMSNYQVVIVKEAQNIKNLSDLEI
                     YLKAPQPSTILVICNKLKPDGKSSSAKLTKLIGLVKQKGVYFESKKLYDYQVADWIVQ
                     YLKPKGIKIDIMAANLLNEYLGTDLSKISHELEKLIITLPAGSNQITTKSIEQNIGIS
                     KDFNRFELTKALGEKNVLKANRIADYFAHNPNANPLILTIAAIHQYFLKVFKLHFIKD
                     KTRENLARELGVNPFFVGEYQSAARKYNPKKCVEIFELLRDYDMRSKGMNNGSTDHGE
                     LLKELIFKIIH"
     gene            complement(4490..5260)
                     /locus_tag="PK839_04575"
     CDS             complement(4490..5260)
                     /locus_tag="PK839_04575"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018710266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HOG72046.1"
                     /translation="MKTKEEIVNNWLPRYTGQAVEDFGTHILLTNFRGYLDYFAKFMG
                     VSIINPDANMPCATAKGITMIDFGMGSSNAATIMDLLSAVKPKAVLFLGKCGGLKRKN
                     QVGDFILPIAAIRHEGTSDDYLPPEVPALPAFNLQRVVSSTIRNHNRDYWTGTVITTN
                     RRVWEYDDDFKDYLRKIRAMAIDMEAATIFTVGFYNEIPSGALLLVSDQPMISEGVKT
                     IESDRVVTDLYLEEQVQIGIESLTRLINNSESVKHLRF"
     gene            5711..6589
                     /gene="fabD"
                     /locus_tag="PK839_04580"
     CDS             5711..6589
                     /gene="fabD"
                     /locus_tag="PK839_04580"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014226406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HOG72047.1"
                     /translation="MKAYVFPGQGAQFVGMGKDLYENSPAAREMFEKANAILGFRITD
                     LMFSGTDEDLRQTKVTQPSIFLHSVILAKVLGDEFSPNMVAGHSLGEFSALVAAGALS
                     FEDGLRLVAARAMAMQKACEKEPSTMAAVLGLDDAKVEEICRSIDEVVVPANYNCPGQ
                     LVISGSVKGVEIACQKLTEAGAKRALKLAVGGAFHSPLMEPARQELEAAIMSTPFSKP
                     ICPVYQNVTAMPTTNPDEIRKNLIAQLTSPVRWTQTVQNMIADGATSFVELGPGNVLQ
                     GLIKKIDRSVVAESRQ"
     gene            complement(6746..7159)
                     /locus_tag="PK839_04585"
     CDS             complement(6746..7159)
                     /locus_tag="PK839_04585"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOG72048.1"
                     /translation="MSTTSTIIYQGNLRTVATHTRSGEQISTDAPVDNQGKGEFFSPT
                     DLLATSLGACMVTIMGISAQTHGFNIDGTRLEVQKIMGTNPRRVVEVIIDLYFPSDYS
                     DKHKRIIEAASRECPVAQSLHPDLKQTIRFHYAEN"
     gene            complement(7259..8596)
                     /gene="gdhA"
                     /locus_tag="PK839_04590"
     CDS             complement(7259..8596)
                     /gene="gdhA"
                     /locus_tag="PK839_04590"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019149313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HOG72049.1"
                     /translation="MEVSKLMAELEKKHPGEVEYLQAVREVLESIEEVYNQNPQFESA
                     KIIERLVEPDRIFTFKVPWTDDEGKIHVNLGYRVQFNNAIGPYKGGLRFHPSVNLSIL
                     KFLGFEQIFKNSLTTLPMGGGKGGSDFNPKGRSDAEIMRFCQSFMLELWKYIGPETDV
                     PAGDIGVGGREIGFLYGMYKKLAHENTGVLTGKGLNWGGSLIRPEATGFGAIYFVQEM
                     LATRNIKLNGKTVALSGFGNVAWGAALKATELGAKVVTISGPDGYIYDEAGVSGEKIN
                     YMLELRNSNQDIVAPYAKKFPGSKFVAGKKPWEVKVDIALPCATQNELNGDDAALIIK
                     NGSICVAEVSNMGCTPEAVEAFTKAKFLFAPGKAVNAGGVATSGLEMTQNSMKLNWPK
                     EEVDVRLHQIMKDIHNACVKNGTGKDGYVNYVKGANIAGFLKVANAMLDQGLV"
     gene            complement(8765..11758)
                     /locus_tag="PK839_04595"
     CDS             complement(8765..11758)
                     /locus_tag="PK839_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008616814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOG72050.1"
                     /translation="MSEENSEQSYLNSYQLDVYDTSFDKLMQKRIYKVLIICSSYDAF
                     MLEEDGRIDEQIFNEYVSLNLRYPPVFIQANTAREALRVLQEEKIDLIISMLNVGEID
                     PFRLAKILKGKYPNVPIVLLTPFSREVSLQIQKEDLSSIDYVFCWLGNADIMLAIIKL
                     IEDKMNADHDILEIGVQAIILVEDSIRYYSSFLPNIYKIVFRQSRSFMKEALNEHHRM
                     LRMRGRPKILMATNYNDAIALYNRYRNNVLGIISDISYKVDNKRDHLAKSGLRLCRVI
                     RENDPNIPFLLQSSDRNNKVYADEMGVGFLHKYSKTFSIDLRNYMINNFGFGDFVFRD
                     PDTLAEVARASDLSQLQQLILTVPDRVLVYHTSRNEISRWLYARAIFTIAELFKPMTL
                     KDFNSIDDVRSFLYKAISAYRLSKGRSLIANFDRNTFDEYKFFTRIGEGSIGGKARGL
                     AFINQVIRKHRLFNKYEGIIISIPRTVVVSTEIFDEFMESNNLYDTALQDISDENVLK
                     AFLNARLPERLKVDLRKVISVFTRPLAVRSSSKLEDSHYQPFAGIYSTYMVPRSSDSE
                     KMLYMVCQAIKAVYASVFYRSSKAYMNATQNVIDEEKMGIVIQEICGNEYGSWYLPTL
                     SGVARSINFYPVGSEQPFDGIASIGFGLGKLIVDGGVALRFSPKYPKKILQLASPETA
                     IRQTQKYFYALNTDPDTFEPMVDDGMNLLKLEIKDAYSIPDLRHTVSTYDMQSGMLRD
                     GFYDEGLKLVTFAPILKHGIFPLAEILTDLLSIGQKEMNNHIEIEFALNMSPRGGTPK
                     MFNFLQIRPIVDTDQSEKFNWEEVDFSKAIIFSNSALGHGLVKNIQDFVYVKPDRFNP
                     AKTIEIAREVEEINNKFIELQRNYVLVGPGRWGSSDPWLGIPIKWSQISEARVIVESG
                     LDSFRVDPSQGTHFFQNLTSFGVAYLTINPFLNDGIYDIEFLNAQPAFLETTFCRWVN
                     FSKPLTIQVDAKNNKGIILKSNGNS"
     gene            complement(11990..12412)
                     /locus_tag="PK839_04600"
     CDS             complement(11990..12412)
                     /locus_tag="PK839_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOG72051.1"
                     /translation="MMRRRSTLPSIVDEFFGDDLWNRFFDDTESVTVPSVNIKEGKDE
                     YAIEVAAPGFDKKDFRIDLNNNVLEISSEKEVKEENKDEKVMRREFRYSSFKRTFTLP
                     DTVDTEKIKASYKDGILSISVPKKDEAKVKPTKQIAIS"
     gene            complement(12705..13385)
                     /locus_tag="PK839_04605"
     CDS             complement(12705..13385)
                     /locus_tag="PK839_04605"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HOG72052.1"
                     /translation="MKRLLFISLLLATAVVDVKAQYSRASLWTSDMERFAREDSTHGC
                     KANCVLFVGSSTFTRWTTAVADFPESNILNRAFGGSTLTDLIYYFDQIVLPYNPLQVV
                     IYEGDNDLAETSKSVGEFIDDVTTITRLVNIHFPRAQILLVSIKLSPSRKEYFSKYLK
                     ANELMKQMADSSEYIDFVDTCTPMLNPDGSPNEDFFGSDLLHLNEKGYLLWKSLLYPR
                     LMKAGQRE"
     gene            13722..17336
                     /locus_tag="PK839_04610"
     CDS             13722..17336
                     /locus_tag="PK839_04610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOG72053.1"
                     /translation="MVEKASKTKSIATDSALGEEQGVVEFDKLKSLVLQNESSIYVVQ
                     SFRVVFCNQSFSDLTGYTEQEVKEILFLDIVHPKDRKLIKILFQNDFQEIRQHKSNSF
                     TLRILTRDNELRWIKSHISLIAWEGKSALLCTSYDITQQKEAEENLADLEQNFGILAN
                     TFGDFIFVLNHNHNIIQANRAVIDRLGYQEHELLLESFVNLHAPSDRSTVRSLLLGVF
                     AGTHCSYSSFLLAKSGYRIPVEVRMVKGMWKRKASIFAIAQDITPRIEAEREVKASEE
                     KFSKAFNTGAVMMAICTMDGGTYLDVNEAFLQKTGLKRDEVIGQSLHDQKIYSDVNRL
                     NQLLAQIREKGKVNNVEVGISNQRGEELTLLLSAEVISIQENDCIMIAMNDITERKRM
                     EEEITNSRIQLKGILDNLPFIAWLADNKGRYLLVNKSFTSFFNTDEGRVLGKKHGDFW
                     AKAHSANLRKHEVEAAKAKKSLSWEAFEKNDLQEEWWEHFVTPVINAENEVIATTGLA
                     RIITDQKLIRLQIEQNLERQKFLTEISYLFNTGVKFETKLNQSLQKVSGLLKLKRAFI
                     VERIHSTTPSPKVFEWHDSTANLDQDALISSLDKYSPAIFDHLNQTSQLVADICSTEE
                     PSVNSLSKALSAKNLFIYPLISEGCCIGFIGVEYSSYNPQTTGNNREFMLTFSSILTA
                     ALEGQSKESKLLESEQRFRTFSELLPEMVFEANDRQVIVFTNNNLLEAFGFSKRKLPA
                     SLKIHDLFMASEHDKLTNHLAKSAQNGQQQSAELLAQRRDKTTFPTLTHVRRVKLSKS
                     KYRYIGVMVDITMQKQQEAELLKAKEQAEAAGRAKERFLSTMSHEIRTPMNAIIGMTN
                     ILLEENPATSQLVNLNALKFSAENLLTLLNDILDFSKIEAERVDLLINETDIHSLHAG
                     ILSSYAQLAAQKGIKLTGSISPLIPHKLLADRVRLNQILTNLVGNAIKFTSNGGVDLS
                     ISLQRQLKKSVWVKFTVTDTGIGIPKDKQKKIFTEFTQVHDQGGKYGGTGLGLAISNR
                     LVNLMGGQIRLKSVFGKGSTFYFSIKLDLEPYHETPEPAEAPEIKLDPTRSYLVLVVE
                     DNELNTLIVQRFLTGWGINNHHAANGVVALEMAKNNRYDLVLMDLEMPEMNGYEATKA
                     LRRLKGKTNNSVPIIALSASAMLDVKRKIFSIGMNDFILKPFKPADLKKKVLFYLSKV
                     "
     gene            17344..18726
                     /locus_tag="PK839_04615"
     CDS             17344..18726
                     /locus_tag="PK839_04615"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOG72054.1"
                     /translation="MRRFIVFFAAVSSLSFFSLEKNAAQVTIELRVKESIGSTSKLSV
                     YTGKEYVTVDSCRPTIEGIYRFNLPETAPLGLYKLTVGKSGSFDLIVSGEKHVALQTV
                     AFAVEDSLRVIDSDENKVFFDYIKLKKTSEQQHWLIKSLLQYHTSGDDVYEKLVDEQR
                     RLTDSFDSRASELAQAYPSLFVSSYINLDTKLQATGTKSDCNSRRMKADIWWNGVNLL
                     DNRLLNTPILIPRLWSYIESLLCEDELGMEEQDSIFVHYIQKLFALPLSEEIRNKFAE
                     TLADGFAETDYFEVIDYLAHAGGKAAATVFDNPDFKTRLELERPLMVGSKAHDFTAKL
                     MEGEKFKLSRSVSRYELIVFWSVWCPHCVETLPKIRSIYEEFRHKGFDVVAVNIDEEQ
                     EPWKEFTSNNSLQWHNTQVPFSSNSPIPEYYNVDGTPKMFLVDNSLRILSRPTSAAQL
                     HATLRKLYGE"
     gene            complement(18831..20723)
                     /gene="speA"
                     /locus_tag="PK839_04620"
     CDS             complement(18831..20723)
                     /gene="speA"
                     /locus_tag="PK839_04620"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008767598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HOG72055.1"
                     /translation="MRKWRIEDSAELYNINGWGVNYFSINSNGNVVVTPKNNGVEIDL
                     RELVDELQVRDVSAPMLIRFTDILDNRIEDMSRSFEVAAQEYGYKGQNFIIYPIKVNQ
                     MRPVVEEIINHGKKFNIGLEAGSKPELHAVIASNPDNESLIICNGYKDEDYIELALLA
                     QKMGKKIFIVIEKQNELKLVARVSQRLKIKPNLGIRIKLASVGSGKWEDSGGDVSKFG
                     LTSSELIQALEFLEGNGMKDSLKLVHFHIGSQVTKIRRIKLALREASQFYVQLYLQGF
                     NIEFVDIGGGLGVDYDGTRSSNSESSINYSLQEYVNDSVSTIVDVSDKNNIPHPNIIT
                     ESGRSLTAHHSVLIFDVLETASLPSWEENEDIGENPHELVSELYEIWDKLNQSKMLES
                     WHDAQQIREEALDLFSLGLLDLKTRAQVERLFWSIARDINQMTADIKHVPDEFRSLPK
                     LLADKYFCNFSLFQSLPDTWAIDQIFPIMPIQRLNEKPDRAATLQDVTCDSDGKIDNF
                     ISTRNISYYLPVHTVKPNESYYIGAFLVGAYQEILGDLHNLFGDTNAVHVSVNDKGYE
                     IDQIIDGETVAEVLEYVQYNPKKMVRTLETWVTTSVKAGIISAKEGKEFLSNYRSGLY
                     GYTYLE"
     gene            complement(21051..21965)
                     /locus_tag="PK839_04625"
     CDS             complement(21051..21965)
                     /locus_tag="PK839_04625"
                     /inference="COORDINATES: protein motif:HMM:NF040144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HOG72056.1"
                     /translation="MRTKAISMIHGRHARLTRSAILFLALTCIGKVAGAQENIKPAIP
                     AKDFTTRIWLGSQLLPGYGQIANRQYWKLPVLYGGMGSMIYLGVKANQTYLSRLSAYN
                     SLTSTDPNRELYKERMVKQRQTRNLYIAAAGAFYVAGAMDAVLVYNNSKHSPAAATIL
                     STLVPGMGQAYNQKYWKIPVVYGGLATLYYLVSWNDRYYSRFKTALKLNIDGDDTTVD
                     EFGGARTADELRYYMDSYRRNRDLSILGLTAIYIINILDANVDGFLHDWNVDDNLSFR
                     VEPAIVNPALTATADTHSALGLSCRVTF"
     gene            complement(22003..22908)
                     /locus_tag="PK839_04630"
     CDS             complement(22003..22908)
                     /locus_tag="PK839_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOG72057.1"
                     /translation="MTKKMALGRGLGALIEDSTFESTRNSDVQTPRHAISELVQEVDI
                     ELIDPNPFQPRTNFDDESLSELAESISKLGIIQPITVRLLDGRYQLISGERRFRAAKL
                     AGLKTLPAFIRTADDQGMLEMSLVENIQREDLNAIEVAISYQRLIDECSLTQDALSER
                     VGKKRATISNYLRLLKLPAEIQLGIINSIISMGHARALISIDDASLQLKLYKKVISDD
                     LSVRQTEELVRNTVSEPQIKQSRSKVMPDLAGPELALREHLTNRLGLRADVKPGGKGD
                     GKIVISYNNPDELSVIIDKFTKIEE"
     gene            complement(22939..23742)
                     /locus_tag="PK839_04635"
     CDS             complement(22939..23742)
                     /locus_tag="PK839_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOG72058.1"
                     /translation="MGKVIAIANQKGGVGKTTSAINLSASLAVLEKKVLLIDADPQAN
                     ATSGTGFDIRNIKTSIYECLIDEVNPNDIILNSEIKGLDLIPSHIDLVGAEIEMLNLP
                     NREKILKEVVAKVKDSYDFIFIDCSPSLGLITVNALTAADSVMIPVQCEYFALEGLGK
                     LLNTIKIIQSRLNPELQIEGFLLTMYDPRLRLSNQVVEEVRKHFQQMVFDTIIQRNIK
                     LSEAPSYGKPVILYDAASTGSANYINLARELLQKNNQTQIENEQKIAQD"
     gene            23990..24979
                     /locus_tag="PK839_04640"
     CDS             23990..24979
                     /locus_tag="PK839_04640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HOG72059.1"
                     /translation="MVNSWFVIVNPRAGGGKGEVDWPLIKGLLTKEGIAFGFALTEHK
                     HHAVELTVKAINSGYRQLIAVGGDGTLNEVVNGIFIQQSVPPAEILVGVIGVGTGNDW
                     FRMYEIPYRYADSVRAIKNRKVFYQDVGVVEYYESSVKHKRYFVNTAGIGFDAEVALR
                     TNRLKDLGRHGFLLYLISILRALFSYKTTRINVNVDGRHLQDKIFSITLGICRYNGAG
                     MLQVPFAISDDGMLDVTVIRRISKLDALTSIPRLYNGKILKHSRVTGLRGQIVEIMSI
                     PSTNLEADGESLGETPLRFTVLTRAIGVVVDENFDAKDNRPAVIQEKQTLSAV"
     gene            25296..26345
                     /gene="porQ"
                     /locus_tag="PK839_04645"
     CDS             25296..26345
                     /gene="porQ"
                     /locus_tag="PK839_04645"
                     /inference="COORDINATES: protein motif:HMM:NF033711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system protein PorQ"
                     /protein_id="HOG72060.1"
                     /translation="MFQKCFITVLVLLSSLCGFSQVGGFGAYQFLNLPFSVRSAALGG
                     KVATIDEPDMAMVMLNPALLDSTLHNNLYLSYSRYVDDISYGAFAYGRSSRWGTFGVN
                     IQQVSYGSFIEADETGLVQGTFKANELAVSLMYARTLDSLFSVGISVKPVYSHLERYT
                     SFGLAVDIGGNYHSRNGLFSSGVVLKNIGLMLKGYTRGNPETLPFEIVAGVSQKLEHA
                     PFRFLVTFHQLQNMNIYYESKLQDSETSLGENSSQPGFAERAGREFLSHLIIGMEFVP
                     VKSFYLRAGYNYQRRNELKVPEKVSMVGFSWGFGVKVAKLHISYSRATYHLAGSTNQF
                     SICTNLNNFVSTKPI"
     gene            26446..27135
                     /gene="cmk"
                     /locus_tag="PK839_04650"
     CDS             26446..27135
                     /gene="cmk"
                     /locus_tag="PK839_04650"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HOG72061.1"
                     /translation="MGKKITIAIDGFSSCGKSTFAKKIAERLGYLYIDSGAMYRAVTL
                     EFLNWEAFRNNTPDTSMTQQILSGLQVGFSFNEQKKTYETTLNGKVVEDAIRSPRISE
                     LVSAVSAIPEVREKMVELQRLMGKDKGVVMDGRDIGTVVFPDAELKIFMTADPAIRAE
                     RRYKELQSKGVQASLEEVLNNILERDKLDQTRAISPLRKAEDAIELDNSYMTIDEQME
                     WAEKLIAARIC"
     gene            27273..28154
                     /locus_tag="PK839_04655"
     CDS             27273..28154
                     /locus_tag="PK839_04655"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008618685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HOG72062.1"
                     /translation="MQVDIDQKSGFCFGVVNAINQAEVGLKQNEEFYCLGSIVHNEQE
                     ISRLEKKGLRVVSRDEFSGLSGKKVLVRAHGEPPSTYELAKENDVELIDATCPVVLKL
                     QERVRKAWEQMKIAGGTVIIYGKKGHAEVIGLVGQTDGNAVVVESPNDLTGIDFSKPV
                     EIFSQTTKDAEMFKQIVDVIKEKMQSCFLSSNIPIKVNHTFCGQVANRKKELIGFAAT
                     HDVIIFVGGQTSSNGKMLFQTCRSSNPKSYYVSSANELRPEWFAGANSVGVSGATSTP
                     LWLMEQVAQEIERITMP"
     gene            28279..28641
                     /locus_tag="PK839_04660"
     CDS             28279..28641
                     /locus_tag="PK839_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72063.1"
                     /translation="MKREVRIVGTIFLVALYCFAASVVCIPKLVPLEEPHNGPGYFFN
                     ASSNLLSLTLPSKNPAGSIGLSPIWVLKNQLKDFSYTIKSVELFLSTIFTQYRNVSIS
                     YPIRLRKTDLLYPFQYFW"
     gene            28757..29257
                     /locus_tag="PK839_04665"
     CDS             28757..29257
                     /locus_tag="PK839_04665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72064.1"
                     /translation="MNTGIAIIGVLIVVISIIPFILVSRAKRKKEKRLRSEIAGMAKS
                     HGCSISMLDVLKDYAVGADENKHFIFFYKHQNGNETKLAINLADIQSCRVINSTRTIG
                     GRDSQYVITDKVALCLTPVEKSNPEIHLELYSADDGTQLVGELELVEKWSKAINNQLK
                     SMKPIK"
     gene            29725..31614
                     /gene="ispG"
                     /locus_tag="PK839_04670"
     CDS             29725..31614
                     /gene="ispG"
                     /locus_tag="PK839_04670"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(E)-4-hydroxy-3-methylbut-2-enyl-diphosphate
                     synthase"
                     /protein_id="HOG72065.1"
                     /translation="MNYCKNLYSYSRRETQEVNIGGVPIGGSNPIRIQTMTTTNTNDI
                     LATVEQCIKAAKAGADYVRITTQGVKEATSLSSIKQELLNRGFTTPIIADIHFNPNAA
                     MEAAKYADKVRINPGNFVTSHAKPTGGSGKQQEEADNTALIEAKLVPLLELCRERGVA
                     LRIGVNHGSLSERIMAKYGDTPEGMVESALEFIKICARHNFRDIVVSMKASNVRVMVQ
                     AVRLLAARMLNEGMEYPIHLGVTEAGNGEDGRIKSAVGIGTLLADGIGDTVRVSLTED
                     PENEIPVAKKLVSYFENRSSFGRIPSMDPLPYNPFSYTRRKTIAVGSIGADNPPVVVA
                     NLSLATKITGKLLSAWGWTYNHAANQWEHSELAADYFYIGSTTIDEEVITDGLPLLST
                     ANSSSQILRQGNKPASFNISAQPICQMVTDADVLSGHLFSTVMVSPNTVLILRTGYSN
                     GFAGQRAAFIRLMNNHENIPVIISREYDDDDRESFQLKASADIGGLLVDGLGDGVMVT
                     APAIADEDICSTVFSVLQAARTRITKTDYIACPGCGRTLFNLQQTLEKVKAHTGHLRG
                     LKIAVMGCIVNGPGEMADADYGYVGAGPGRVTLYKGKQVMKKNIPEEDALDELIGLIN
                     ADRGL"
     gene            complement(31815..33257)
                     /locus_tag="PK839_04675"
     CDS             complement(31815..33257)
                     /locus_tag="PK839_04675"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOG72066.1"
                     /translation="MRKFFKMQQQKVSRGLVLALVMVSSFITPFIGASVNLALPLIGK
                     DFGLNAVGMSWVTMAFLLSSAVFLVPIGKIADIAGRKRVFFWGNIIMAISSVLCAISP
                     SGAVLITFRAVQGLGSAMVFATGVALITSVFPPSDRGKAIGLNVTSVYIGLSAAPLIG
                     GLLISWFGWESLFYVPAFLGIFSALSIRMAVRSEWADARGENFDFAGAAIYVPSMTLF
                     MYGFSQLPDPVAVCLTILGFIGLIFFVRVELRASYPVFNVRLFRNNRLFAMSNVAALI
                     NYATTFAITFVLSLYLQYIKGLSPREAGLLLIVQPLLMAFVSSWSGKMSDKHDPRILA
                     SLGMGIIAVGLALLIPIGKNTSITYLIVAQAILGFGFGLFSSPNTNSVMGSVEKKFLG
                     IASGTVATMRLTGQMASMGIATLILQLYIGKSDINPGNYRQFGWSVGTTFTVFVLLCI
                     VGVWASLARGKNNGNSMRELNPENEMERIA"
     gene            33350..34693
                     /locus_tag="PK839_04680"
     CDS             33350..34693
                     /locus_tag="PK839_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009040277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOG72067.1"
                     /translation="MAKILVVDDDSTFCLMLKTFLTKHEHSVVEAFSFDEAVRQIRSE
                     NFDVVLTDFRLPEKSGMDVLSEAKHKSSATVVIMMTGYADIRMAVNAIKHGAYDYVTK
                     PINPDEILTSISRALPGNRKAIDKDGTHNFIFINGNSEHSQEVSRYISIVAPTNLSVI
                     IQGDSGTGKEYIARKIHAESKRSSKAFVPIDCGALPRELAASEFFGHLKGSFTGAIAD
                     KIGQFEVANGGTLFLDEIGNLPYDIQVNLLRAIQERKIKRIGSSKEIPVDVRIIVASN
                     ENLLNMVNSGAFREDLYHRLNEFTIRVAPLHERKDDLLIFAKHFLSLANVELGKGING
                     FDADVLDILMDYTWPGNLRELKNVIRRAVLMEQSNLLTVSSLPDEIVHRSGPGDDDKS
                     AKHVENLRNIKEKAEYDLIISTLEKAKYNKSKAALLLNIDRKTLYNKMKHYGISG"
     gene            complement(34760..35161)
                     /locus_tag="PK839_04685"
     CDS             complement(34760..35161)
                     /locus_tag="PK839_04685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72068.1"
                     /translation="MMERDYILRVIQEFFKLINGLLGKLNEDNADEIHKQLQEVYSAY
                     FDRSCQFFYRLSCDEMITLIDNDDQFRHVEKVEMLSELLYRDGLFTSNDVLRKDLLCK
                     ALALIERIEKQSKSFSVERNDRKVGILAMLN"
     gene            complement(35405..36253)
                     /locus_tag="PK839_04690"
     CDS             complement(35405..36253)
                     /locus_tag="PK839_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72069.1"
                     /translation="MKSKANILALLLVMMATTAIAQDSFIVTRIKGNVVNYRTGTSLR
                     AGDVLQPSDKVTFDNFDSYVISIGQNMGRYMIKLHEPPAPDAIQQLTATVKDVAVPTK
                     RRSLMAERYRPNESVVEDLGSYFGNDKFSVIGNEVLVPVNPDKFQLNDSRFIVFYYRV
                     NNNPVSKKIGYEQNVLKIEKDKLLNTSAGTISNDEIPNVAVYRYESNTHSSEEITRFN
                     LIFVDKVQLTNEFLTILPILHRQNMSKDDIKKYLIEYYYDFYGVTDSKSIKKFVDDLV
                     DGSAGN"
     gene            complement(36371..36694)
                     /locus_tag="PK839_04695"
     CDS             complement(36371..36694)
                     /locus_tag="PK839_04695"
                     /inference="COORDINATES: protein motif:HMM:NF016152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HOG72070.1"
                     /translation="MNVEEIRDYCLSKPGATESLPFDDETLVLKVGGRIFAMISLSGD
                     SSLTLKCDPEIALELQEKYTFVEPGYHMNKKFWITLRDTTLIPRNLVMQWIDSSFVLV
                     AAGKK"
     gene            complement(36699..37079)
                     /locus_tag="PK839_04700"
     CDS             complement(36699..37079)
                     /locus_tag="PK839_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HOG72071.1"
                     /translation="MGQIVNTDKRKDLGTLGEDSAAEYLVSKGYKILHRNWHFGHKEL
                     DIVARDGNMLVVVEVKTRTSDYWEEPKEAVRRKKQKRLVEAADAYVCHYNLDTEVRFD
                     IVSVVIANGSLAIEHIEDAFYPTL"
     gene            37184..37861
                     /locus_tag="PK839_04705"
     CDS             37184..37861
                     /locus_tag="PK839_04705"
                     /inference="COORDINATES: protein motif:HMM:NF025729.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4412 domain-containing protein"
                     /protein_id="HOG72072.1"
                     /translation="MIRLKEQKWGHFRRFILVIPMLLLFPFNLLSNIDNFEGKIKFLS
                     EGVYDTLSMVLSVKNDLVRMDQYSGKNGLEISYLFDLRSGKVFALSGREKVYKEIYLH
                     DQSKKRRETDVTIIKSQNSMLINGTTCYQWRVKNRQSMTEVTYWVAQSGFDFMEPLQR
                     LIGQLQTPFNLFTLCPDIKYFFPLMVVDRTIFRKFRCSLRVVDIKRVVLPDNLFAIPR
                     GYRELLG"
     gene            complement(37827..38777)
                     /locus_tag="PK839_04710"
     CDS             complement(37827..38777)
                     /locus_tag="PK839_04710"
                     /inference="COORDINATES: protein motif:HMM:NF036772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HOG72073.1"
                     /translation="MENITPPYLKPGDTVGIIAPARFVSHEDVNPAIRFLTARGFIVK
                     TAPSLFAKHFQFAGSDSERASDFMEMVNDSNVKAILCARGGYGTVRILDRINLRKLQL
                     KPKWIVGYSDITVLHSIITSWFGIETLHATMPVNFPKNINDSESASSMVDVLMGRNPA
                     YSIPHHPLNRKGTATGFLTGGNLSILASLIGTDADTSTQERILFIEDVDEYLYHVDRM
                     MLQLKRAGKLKSIAGLVVGHFSEMKDNNIPFGLDAYQIISEAVKEYRIPVCFGFPAGH
                     QEPNLPLIMGRKVQLVVNSQGASLTFHESSSQAILDTHGE"
     gene            complement(39023..40132)
                     /locus_tag="PK839_04715"
     CDS             complement(39023..40132)
                     /locus_tag="PK839_04715"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOG72074.1"
                     /translation="MNKFTRLIALVAIGLILCSWGQTGHYIISSKSSLSFNLEMEQFQ
                     TWASYLAEHASDADKRKDEDPNEAPKHYIDIENYPEFINAGYIPQTLNEAITLHGSYN
                     VYDWGILPYATKATFDSLKSNMARLNWDRAKFFAADLGHYVADGHMPMHITKNYNGQL
                     TGNDGIHSRYESTMVNAREQQLSQYEGAEIHQIDNVTQYILNYLYANYKYVDSVLAAD
                     NYATGISSNYSSTAYKNALWEKTGEFTTMLFANASHALAELMYTAWKEAGSPSLTGVD
                     MIGQDAEGYYLYPIRPNPARNEVSISFHLEKPCVANLIVCDGNGKIIATLAQGELGGG
                     NQTITWLTRNQPNGIYLVALRANGRQFSQKLVVNH"
     gene            40718..41230
                     /locus_tag="PK839_04720"
     CDS             40718..41230
                     /locus_tag="PK839_04720"
                     /inference="COORDINATES: protein motif:HMM:NF018047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA family protein"
                     /protein_id="HOG72075.1"
                     /translation="MGINTPSPIGYIEEKQFGIIRRSYYVCAYQPYRATITGILGSND
                     TDRETIIREFTRFTFTKLHQQGVLHKDYSGGNILVEDLGEDSYRFSIIDLNRMNFKKK
                     VSTKQGLKNLRRLTLDITVNDIVTITSEYAALAGNSNPKQTLFWFLKYHLDFVRFKNF
                     KSWYKKFKRG"
BASE COUNT        11468 a         9638 c         9440 g        11056 t
ORIGIN      
        1 ataaggctaa atcaccctgg tactggctat ttggtggaag ctttcgctcc agctcgccat
       61 gcgcaatttc cttaaagtaa agtttgcacg catttacaac ctgattctga tacgagaggc
      121 tgtacccgtt ttttaaaatg taatcattaa caaagcgcac catgtcgtta ttggttgcat
      181 cggtcgctgc tttgggttga aggaatatta gaaaactcct tagcgtttca aggtaggttc
      241 tggttgtgtt tgtgctatac cgcctgtata aaagccatgc gttgaattta ttcaactcgt
      301 caggaatgct gcaccaaacg gcaccctttg atttctgaat agcctccaca ctttcgctta
      361 atgagcaaac atctataacg gccatacttt caagcgcctc cctcaaccag tcaatgttaa
      421 aactccctgt agggaatata ccatttatgg tctgtctggc tccatttggc acagccaagc
      481 ttccttacag cgtcaatcag cgttttgtca aactcgaaat tcaggaacac taccttgttg
      541 ttcctatgcg tgccctgcct gcaggtgata accttgagtt cggttgtcat gtttcaagtt
      601 gctgattggc caacagtggt aaaactttct tatttaggtc aaactaataa aaatattcaa
      661 tggatacagc taaatggggt gcattttcaa tgtagattgc tcgtaattct tcattttatg
      721 tagctgccat tcggccaagt tatatatata ccccctcggc ctgctcggtt tccagctcca
      781 accccacccc tgcctgtaaa gctaaagtta cgtaaatttt gacaggtagc ggataacctt
      841 caaggatttg gcataatttt agaagataat aggcggtttt tctcacgtaa ccctaaagcc
      901 ggaaaggagg tgcaccatgg caaagtttaa accatcggaa atggatttct tcaagttcga
      961 cttcaggcag attccgccaa aggccgggcg gttgcttatt gcccagccct cgcttaccga
     1021 tccgttcttt aagcggacag tgatatacct ggtggagcac gacgacgagg ggtccatggg
     1081 cttcatcctc aaccgcaagc tggccatgag cttgaacgac ctgctgaaca atttccccaa
     1141 cgtggaggcc aacgtctcgg tgggcggccc tgtgggctcg gataccgtga actttatcca
     1201 cacgttcggc agtaccattc ccaacacgct cgacctgggc aacgggctct acctcaacgg
     1261 cgatattgac gctgttaagg ccttggccat tgccggtagg ctcacccggg aaaatttcag
     1321 gatattcatc ggctactccg gatggggcaa ggatcagcta aaaagggaga tcgacgagga
     1381 ttcatgggtt gttgccaact tcgactctca gcttatggcg aaaggagctt atgatagctg
     1441 gtactttgcc gttcagcagc tggggtcaag gtttaaggcg tggaccctct accccgaaaa
     1501 tccagcccta aactgatcgg gaaaggtgaa gcggtttagt gcctcgctta gcgttctact
     1561 cacccggttg aagtaccttc gggttccgat gcctaccacc catttaatcc ccgatatggc
     1621 attggtgagg aaaacctcgt cgcagctgtc gaatgccgac gggttgatgg caacctggtt
     1681 gttaacctct attccaacag ttggggcaac agtgcacacc agctcccgca tgatacccgg
     1741 tatgcacccc tcagccagcg agggggtgta gagctttccg cctcttacca cgaacacgtt
     1801 ggacgaaaga gcctcggcaa cccttccctg ctcattcagg atgatgcaat cgtccaggtt
     1861 gttggccatg cagtatagcc cggccttaac aaaaagtagc gcattgcagc tcttaacgcc
     1921 cgaaagcgta ttcagcggtt ttttgatctc atcgtaaatt ccaactatgc agccctgctg
     1981 gtttagggtg tacattttgg ctgccaacgg ctgtgtgtca ataagaacgc ttgcgttgga
     2041 ggtagaaggg atatagaatc ccccatcgtc gcggtaaacc gtaaccctca cccgtgcact
     2101 gtcaaaaagt cggtttttgt taagcagctt cgtaatggct agttcaaggt tttgctcggt
     2161 aagccaagtt ggtgcaacga gctccaggat tttcattccc tgcagcaggc gcctgaagtg
     2221 caggggaagg tgacgtggtt gcgttccaaa ggcgtgaatg gtttcaaaaa ccccatcacc
     2281 atagcgaaac gccctgttct caaaattaat gacagggtta ccgccgtcga aaaactggcc
     2341 attaagccaa atgtagttcc tgcttgccat tcggcattaa ctttcagata catacccaag
     2401 cgcagcctcg aaaagctgta gctgaacccc ctcgtatgtc aactcgtctg gagggctatc
     2461 aaacattcct attttctgaa tctcactctg gggaagagcg ccaagcttgg ttaccgcggc
     2521 gtaaaacatc cggcaatcac ttctggcgct tccctgctgg atggtgtagt cgaaaagcgg
     2581 aaccatgctg aaatccaagg ctccagtctc ctcaaacagc tccctgctgg cggtttcatc
     2641 tggttcttct ccggtttcaa tatgtcctcc tggcagctcc caggttgagc ggttacggtt
     2701 catcacccaa atccaccctc cacggtatct ggccatgatg gcaacaagcc ggtgttcccc
     2761 actctgtagc catccttcac atattttcac aaccaagggt tccatatacc ggcatcaagc
     2821 tacattagtc caagctgctt taaaaaaacg gtaatcagtt tagggctgat gagcagcgga
     2881 tagataaagc caaatacagc acctacgaag tgagcatcgt ggttgatgtt atcgccaccc
     2941 tttcggctca tgtagttgga gtaaatcagg taggcaacgg caaacagaat acctggaatt
     3001 ggcagaatgc ccatgaggta gagcttgtta agcgggtaga aaaatatgca gaagaatatc
     3061 attcccgaca cagcccccga agcgccaacg ctctggtagg agtaatcgtt tctgaacttt
     3121 ttgagggttg taagggtggc aacaactgca ccacccaggt agagcattag gtagtgcagg
     3181 gcaggatgac cgataacccc ctccatgttc agctgcctta ggattgactc caccgccttc
     3241 ccaaacgaca gcagcacaat catgttgaca atcaggtgca ggtagtttgc gtgaactaaa
     3301 gcgtgggtaa tcaaccggta ccactgcctg ttccggaaca ccaggtaggg actcaggagc
     3361 aatcgctgca tcaactcccc gtttcggagc gcaacagccg ataccagcga ggtgatgatg
     3421 ataatggcgt aggtcatgct agtgaattat cttaaaaatt aactccttca gcaactcacc
     3481 atggtcggtt gatccattgt tcattccttt tgaacgcatg tcgtagtcgc ggagtagctc
     3541 gaatatttca acacacttct ttggattgta ctttcgggca gcgctttggt actcgcccac
     3601 aaaaaaagga ttaacgccca gctccctggc caaattctct cgtgtcttat ccttaatgaa
     3661 gtgcagctta aagactttca ggaagtactg gtgaatggca gcaattgtta aaatcaaagg
     3721 gtttgcattg gggttatggg caaagtaatc ggcaatacgg ttggccttca gcacattctt
     3781 ctcacccagt gccttggtca gctcaaagcg gttaaagtcc ttgctaatac caatgttctg
     3841 ctcaatactt ttggtggtaa tctggttgct tccggcaggc agggtaatga ttagcttctc
     3901 cagctcgtgg gaaattttgc ttagatcggt acccaggtac tcgttgagca ggttggccgc
     3961 catgatgtca attttaatgc ctttgggttt caggtactgg acaatccagt cggccacctg
     4021 gtaatcgtaa agttttttcg actcaaagta cacccctttc tgcttaacca atccaatgag
     4081 tttggttagc ttggcagagc tgcttttccc atcgggctta agcttgttgc agattaccag
     4141 aatagttgag ggctggggtg cttttaggta aatttccaga tcgctcaggt tcttaatgtt
     4201 ctgggcttcc ttcactatca ccacctgata gttggacatc atcggaaatc gcctggcggt
     4261 gttgatgaca tcggcaatgg taacgtccct gccgtacaca atggtaaggt taaaatcttt
     4321 ttcggttgcg gacagcacgt tgtcggcaag gtagtcggat aacttgtcga tgtagtagtg
     4381 ctcttcgccc gataggaggt atatgggctt ataaatttta tttttaaact cctccagaat
     4441 tttagtgtag gaatcgaggg tggtgctttt agccattgcc ggacaaaggt tagaaacgaa
     4501 gatgctttac ggattcgctg ttgttgatta agcgggtaag tgattcaatg ccgatttgaa
     4561 cctgctcttc aaggtaaagg tcggtaacca ccctgtcgct ctcgatagtt ttaaccccct
     4621 cggaaatcat gggctggtcc gaaacgagca gcagggcacc cgaaggaatc tcgttgtaaa
     4681 agcccaccgt aaatatggtt gctgcctcca tgtcgatggc catggctcta attttccgaa
     4741 ggtaatcctt aaaatcatcg tcgtactccc aaaccctacg gttggtggta ataaccgtgc
     4801 ctgtccagta gtcgcggttg tggttgcgaa tggtcgagga aaccacccgc tgaaggttga
     4861 atgcaggcag ggcaggcact tcggggggca ggtagtcgtc ggatgtgccc tcgtgcctga
     4921 tggcggcaat aggtaggatg aagtcgccaa cctggttctt ccttttcaac ccgccgcatt
     4981 ttccaaggaa aagaacggcc tttggcttaa cagcgctaag cagatccatg atggttgctg
     5041 cgtttgaact ccccatgcca aagtcaatca tggttatccc ctttgcggtg gcacagggca
     5101 tgttggcatc aggatttatt attgaaaccc ccataaattt ggcaaaataa tccaaataac
     5161 ccctgaagtt ggtcaaaaga atatgggtac caaaatcctc aaccgcctgg ccagtatagc
     5221 ggggcaacca gttatttaca atttcctcct tggtcttcat tccgtaatcg ttattagggt
     5281 taaaagtatg ttgatggagc gctactcatt taggcctctt catttcggct ttcatgcaaa
     5341 agtagtttaa aaattttatt ggggcagcat aacggcatta ttaccatgaa gcaatgtttt
     5401 ttaaccattt acaggtttac ccatgcggct aacttgacaa tctgaaaaaa tgccctactt
     5461 ttgtcagccg gatggatggg tcatggccgg gaaaattttc ccagggggat ttatttttgc
     5521 ttgacaaaaa tacagtcata ataccttccg cttaactttt ttagaggatt aatgtaaaac
     5581 aagcggcaaa acgaaccaat aaaatttacc gattacaagc gtttttgttg gttttggctt
     5641 cacataaaac tcaaaagact aagtattgac caacgtaaaa gtgtaaacac aaacctaaaa
     5701 ttttctacgg atgaaagcgt acgtatttcc gggacaaggc gcccagtttg tagggatggg
     5761 aaaagatctc tacgagaact ctccagcagc aagggaaatg ttcgagaagg ccaacgccat
     5821 actaggcttt cgcattaccg acctaatgtt tagtggaacc gacgaggatc taagacaaac
     5881 gaaggttacc cagccctcca ttttcctaca ctcggtaatt ctggctaaag ttttaggcga
     5941 tgaatttagc cccaacatgg ttgccggtca ttcacttggc gaattttcgg ccctggtagc
     6001 tgccggtgcg ctgagctttg aagatgggct aaggctggtg gctgcacgcg ccatggccat
     6061 gcagaaagct tgcgaaaagg aaccctcgac catggcggca gtgcttgggc tggatgatgc
     6121 gaaggtggaa gaaatttgcc gctctattga cgaggttgtt gtgccggcta actacaactg
     6181 ccccggacag ctggttattt ccggatcggt aaagggggtt gaaattgcct gccagaagct
     6241 taccgaagcg ggtgctaaac gggcacttaa gctggcagtg ggtggtgcat tccattctcc
     6301 ccttatggag ccggcccggc aggagctcga agcggccatc atgagcactc cgtttagcaa
     6361 accgatatgc cctgtttacc aaaatgttac agcaatgcct acaaccaacc ctgatgaaat
     6421 caggaaaaac ctgattgccc agctaacctc cccggtaaga tggacacaaa ccgtccagaa
     6481 catgattgcc gatggggcta catcattcgt tgagctggga ccaggcaacg tgcttcaggg
     6541 gttgatcaag aaaattgacc gcagcgtggt ggccgaaagc aggcagtaat cctttgctgt
     6601 ccaaggttta tttgaaaaat aataactagg ggctacctaa aggtggcccc taaacttggc
     6661 ttcttgacca gtagtctttt acgatatcct ttaccctccc gcaagccaaa ccctggtggc
     6721 ttttaccctt taggtttaaa gctctttaat tttcggcgta atggaaacga atggtttgct
     6781 ttagatcggg gtgtaggctt tgggcaaccg ggcattccct cgaagcagcc tcaattattc
     6841 ttttatgctt gtcggaataa tcacttggga agtagaggtc aataataacc tcgacaaccc
     6901 tacggggatt ggttcccatg atcttttgca cttcaagccg cgtcccgtca atgttaaagc
     6961 cgtgggtttg tgcagaaatc cccataattg taaccatgca ggcgccaagc gacgtggcca
     7021 gcaggtcggt tggagagaag aactcccctt tcccctggtt gtcaaccggt gcatcggtac
     7081 taatctgttc gcctgaacgt gtatgtgtgg caacggttct taggttgccc tggtaaataa
     7141 tggttgaagt tgtgctcata ttatgtaaga ttgtgattac cctttcgtag ctataaacgc
     7201 ataaaagggc tcaaagttga gcccttttat agtaaaatca tccaaacagg aagctggttt
     7261 aaaccaagcc ctggtcgagc attgcattgg caaccttgag gaaacctgca atgttggcgc
     7321 cttttacata gttaacatac ccatccttgc ctgttccgtt ctttacacat gcgttgtgga
     7381 tatccttcat aatctggtgt aaacggacat caacctcctc cttgggccag ttaagcttca
     7441 tgctgttctg ggtcatttcc aggccggagg tggcaacacc gccggcatta acagccttac
     7501 cgggggcaaa aagaaactta gccttagtga aggcttcaac tgcctcgggt gtgcaaccca
     7561 tgttggaaac ctcggcaacg caaatggagc cattcttgat tattaaagct gcatcatcgc
     7621 cattcagctc attctgagta gcacaaggaa gggcaatgtc aacctttacc tcccagggct
     7681 tcttacctgc cacgaacttg gagccaggga acttcttggc gtagggtgca acaatatcct
     7741 ggttagagtt gcgcagctcg agcatgtagt taatcttttc accgctaaca ccggcctcat
     7801 cgtagatata tccatcggga ccagaaatgg taaccacctt agctcccaac tcggttgcct
     7861 ttagggcagc cccccaggct acgtttccaa aacctgaaag cgcaacagtt ttaccattta
     7921 acttgatatt cctggtggca agcatttcct gaacaaagta aattgcacca aacccggtag
     7981 cttcgggacg gattagcgaa cctccccagt ttagaccttt accagtgagc acaccagtat
     8041 tctcatgggc aagtttttta tacattccgt agaggaatcc aatttcgcga ccgccaacac
     8101 ctatgtctcc ggctggaaca tcggtttcgg ggcctatgta cttccagagc tcaagcatga
     8161 aagactggca aaaacgcata atttcggcat cgcttcgacc tttagggttg aagtcgctac
     8221 ctcccttacc accgcccatt ggcagcgtgg tgagagaatt tttaaaaatc tgctcgaatc
     8281 ctaaaaactt aagaattgaa aggtttacac ttggatgaaa gcgcaatcca cccttatagg
     8341 ggccaatggc gttgttgaat tgaaccctgt aaccgagatt tacatgaatc ttcccctcgt
     8401 catctgtcca gggaacctta aaggtaaaaa tacgatcggg ctctaccaaa cgctcgataa
     8461 ttttagcgga ctcaaattgg gggttctggt tgtaaacctc ctcaattgat tccagaactt
     8521 cacgaactgc ttgcaagtac tccacctcac ccgggtgttt cttctcaagc tctgccatta
     8581 gtttactaac ttccattgct taattttttt aaggtactac tcaaaacaat ctatgaaagg
     8641 gcatcattta tatcccctta aaagtttttc aaaaatgatg taaaaaaata atatttcaaa
     8701 agaaataggg gtttaaaaat atgatattaa gcagattttt acccaaatcg ttaacattta
     8761 aaacttacga attcccattt gattttaaga tgataccctt gttgtttttt gcatcaacct
     8821 gtatggtaag gggtttagaa aaattcaccc accggcagaa ggtagtttca aggaaggctg
     8881 gctgtgcgtt gagaaactct atatcgtaaa taccatcatt gaggaatggg ttaatggtga
     8941 ggtaggcaac accaaaagag gtaaggttct ggaagaaatg tgttccctgg ctgggatcaa
     9001 ccctgaagct gtcgaggccg gattcaacaa taaccctggc ttccgatatt tgtgaccatt
     9061 ttattggaat acccagccaa gggtcgctgc tgccccacct gcccgggccc accagcacgt
     9121 agtttcgttg caactcgatg aatttattgt tgatttcctc cacctcacgg gcaatctcta
     9181 tagttttagc cgggttaaac cggtcgggtt ttacgtaaac aaaatcctga atatttttca
     9241 ccaacccatg cccaagagcg gaatttgaga agattattgc cttggaaaag tcaacctctt
     9301 cccagttgaa tttctccgat tgatcggtat ccacgatggg gcgaatttgg aggaagttga
     9361 acatttttgg agtgcctcct cgggggctca tgttgagggc aaactctatt tcgatgtggt
     9421 tattcatctc cttttgcccg atgctgagca ggtcggtaag aatctcggca agggggaaaa
     9481 ttccatgctt cagaatgggg gcaaaggtaa ccagcttaag gccctcgtcg tagaacccat
     9541 cgcgtaacat gccgctctgc atgtcgtagg ttgagacagt gtgcctgagg tcgggaattg
     9601 agtaggcgtc tttaatttcg agcttgagga ggttcattcc atcgtccacc atgggttcga
     9661 aggtatccgg atcggtgttt agggcgtaaa agtatttttg ggtttgccta atagctgttt
     9721 cgggggaggc aagctgaaga atttttttgg ggtatttcgg tgaaaatctt agggcaactc
     9781 ctccgtcaac tatgagcttc cctagcccaa aacctatgct tgcaattcca tcgaacggct
     9841 gctccgaccc aaccgggtag aaattgatgc tgcgggctac tcccgaaagc gttgggagat
     9901 accagctgcc gtattcgttg ccacaaattt cctggataac aatgcccatc ttttcctcgt
     9961 caattacgtt ctgcgtggcg ttcatgtatg ccttacttga gcggtaaaat accgaggcat
    10021 acaccgcttt aatggcctgg caaaccatgt aaagcatttt ttcggaatcg gaggatcgcg
    10081 gcaccatata ggtggagtaa atgccagcaa agggttggta gtgggaatcc tcgagtttgc
    10141 tcgaagatct tacagccaat ggccgtgtga aaaccgatat aacctttcgg aggtcaacct
    10201 taagccgttc gggtagccgg gcgttcagaa aagcttttaa aacgttttcg tccgagatat
    10261 cctgaagagc tgtatcgtag agattgttgg actccatgaa ctcgtcgaag atttcggtgc
    10321 taaccaccac tgtacggggt atgcttatga taatcccttc atacttgtta aaaagcctat
    10381 gtttgcgaat gacctggtta ataaatgcaa gcccgcgagc ctttccgcca attgaaccct
    10441 ctccaattcg ggtaaaaaat ttgtactcat cgaaggtgtt cctgtcgaag ttggcaatca
    10501 ggctccttcc cttgctgagt cggtaggctg aaatggcctt gtaaagaaag ctgcgaacat
    10561 catcaatgga gttaaagtcc ttgagggtca tcggtttaaa tagctcggca atggtaaaaa
    10621 tggcacgtgc gtaaagccac cgcgatattt cgttacggct ggtgtggtaa accagcaccc
    10681 tgtcgggaac ggttaaaatg agctgctgga gctgacttag gtcggaagcc cgggccacct
    10741 cggctagggt gtcggggtca cgaaaaacaa aatccccaaa accgaagtta ttaatcatgt
    10801 agttccgcag gtctatgctg aaggttttcg agtacttatg caggaaacca actcccattt
    10861 catcggcata aaccttattg ttcctgtccg atgattgcag caggaaggga atgttgggat
    10921 cgttctcacg aataaccctg cataacctta atcccgactt ggccaggtgg tcacgcttgt
    10981 tgtcaacctt gtagctaatg tcggaaatga tacccagcac gttgtttcgg taccggttgt
    11041 aaagtgcaat agcatcgtta tagttggtgg ccatcaggat tttaggccga ccacgcattc
    11101 gcagcattcg gtggtgctca tttaacgctt ccttcataaa gctgcgcgac tgcctgaaca
    11161 caattttata gatgtttggc aaaaatgagg agtagtagcg aatggaatcc tccaccagaa
    11221 ttattgcctg aacgccaatt tcgagtatgt cgtggtcggc attcattttg tcctctataa
    11281 gcttaattat ggccagcata atatcggcat tgcccagcca gcagaaaacg taatcaatgg
    11341 agcttaaatc ctccttctgg atttgaaggg aaacctcgcg cgaaaagggg gtaaggagaa
    11401 caatgggtac gttcgggtat ttccccttaa gtattttggc cagcctaaag ggatcaatct
    11461 ccccaacgtt gagcatggat atgataaggt ctattttctc ttcctggagt acccgcaagg
    11521 cctccctggc cgtattggcc tgtatgaaca ccggcgggta cctaaggtta agagaaacgt
    11581 actcgttaaa aatctgctcg tctattcgtc catcctcctc cagcataaag gcatcgtagc
    11641 tgctgcaaat aatcagcacc ttgtaaattc gcttttgcat cagcttatcg aacgatgtgt
    11701 cgtacacatc gagctggtag ctgttcaggt acgattgctc tgaattttcc tccgacatgt
    11761 ggtattagct ttcggtcccg atgcgaatat agcaacttta ggtgagattg ttaccctact
    11821 catttatagc cttttgctat gccttaatgg ctggaactat aatgcatgaa tagtgccgat
    11881 ggaagggagt cgaagctaaa acatgctact ataagctggt aggaaaaagg gtgggtaaat
    11941 aacaaaaaaa cccggctcta agtccgggtt tttttgttgc caacagaggt tacgaaatgg
    12001 ctatctgctt ggtgggttta acttttgctt catctttctt aggaacgctt atgcttaaaa
    12061 ttccatcctt gtatgaagct ttaatttttt cggtatcaac cgtatcgggc aaggtgaaag
    12121 tccttttgaa cgaggagtac ctaaactccc gacgcattac cttttcgtcc ttgttttcct
    12181 cttttacttc cttttcgctg gaaatttcaa gaacgttgtt gttcaggtca atacggaaat
    12241 cctttttgtc aaagccagga gcagcaacct ctatggcgta ctcatcttta ccttccttaa
    12301 tatttacgga aggcacggta acactttcgg tatcgtcaaa gaacctgttc cacaggtcgt
    12361 cgccaaaaaa ctcatcaacg atacttggca gtgtgcttct acgtctcatc atcggcatca
    12421 tagctttacc ctccatactt taagttagac attcaatttt taaactctga tacctaacta
    12481 tcaacttgaa tgccaagacg aaaaagcgcc attttgaccg aatggtgtca actttgtctg
    12541 ccatgttgac accaaggcga gaaggagaaa gcgcacaggt gaaaaatgtc agcaccagca
    12601 tcctctattc aacaaaatca tgtccggttt taagctattg acactttggt aatatggcat
    12661 tctctgccaa gcggcagatt tttgggaagc actgtaaaca ggagctattc tctttggccg
    12721 gctttcataa gccgagggta aagcaacgat ttccaaagca agtaaccctt ttcgttaaga
    12781 tggagcaaat cggatccaaa aaaatcctcg ttgggagagc catcagggtt cagcatgggg
    12841 gtgcaggtat ctacaaagtc aatgtattct gaagagtcgg ccatttgttt catcagctcg
    12901 ttagccttaa gatacttact aaaatactcc ttacgcgagg ggcttagctt aatagatacc
    12961 agcagtattt gtgcccgggg aaaatgaata tttaccaagc gggttattgt agtaacatca
    13021 tctataaatt caccaacact ttttgaagtt tcggccaaat cgttgtcgcc ttcatagatt
    13081 accacctgca agggattgta gggtaaaact atctggtcga aatagtaaat caaatcggtc
    13141 aaagtagagc caccaaatgc ccggttaaga atgttcgatt cgggaaaatc ggctacagcg
    13201 gttgtccatc gggtaaaagt ggagctgccc acaaatagca cacagttggc cttacaacca
    13261 tgggtagaat cttccctggc aaacctctcc atgtcgctag tccacaatga agcacggcta
    13321 tactgcgcct taacatctac tacagctgtt gcaagcagca aggaaatgaa tagtaatctt
    13381 ttcataccaa tcaaattcac ctaaaggtta cactaccaag tatcaaaaag ttgaatattt
    13441 atagttaaac tttcacattc gaaacatgtt gcaaacaggg agacaaatca tttcgttgaa
    13501 aaaacgtata attctgcaat aggaatccaa tttgatgata agaacgcggg ttgaaatgta
    13561 acatttccac attacaaata tacctttttg gaatttcaac atacgaacgc aggaacaagc
    13621 tattttccta gtatatcaaa aaatttcaga tcatgtagga attgctcttt actattggca
    13681 aactttttaa taccttttgg caatttttta gtaaccacta aatggttgaa aaagcctcaa
    13741 aaaccaaaag tatagctacg gactccgctc tcggcgagga gcaaggagtg gttgaattcg
    13801 acaagctgaa aagcttggtt cttcagaacg agagcagcat atacgtggtt cagagcttcc
    13861 gtgtagtctt ttgtaatcaa agtttttccg acttaacggg ctacaccgaa caggaggtaa
    13921 aggaaatact attcctggac attgtccatc ccaaggatag aaagctaatc aaaatattat
    13981 tccagaacga ttttcaggaa atcaggcagc acaagtcgaa cagcttcact cttagaattc
    14041 tcacccgcga caacgagctg aggtggataa agtcgcatat ctcgctaata gcgtgggaag
    14101 gaaaatcggc gctgctatgc accagctacg acatcaccca gcaaaaggag gccgaggaaa
    14161 accttgccga tctggagcag aatttcggca tacttgcaaa cacatttggc gattttattt
    14221 ttgttctcaa ccacaaccac aacatcattc aggcaaatag ggcggtgatt gacaggcttg
    14281 gataccagga acatgagctc ttgctcgaat catttgtaaa ccttcatgct ccaagcgata
    14341 ggtcaactgt taggagttta cttttaggag tatttgctgg cacccactgc tcctactcat
    14401 ccttcctgct ggctaaaagc gggtatagaa ttccagttga ggttagaatg gttaaaggaa
    14461 tgtggaaaag aaaggcttcc atatttgcca ttgcccagga tatcaccccc cgtattgagg
    14521 ccgaacgcga ggtgaaagca tcggaggaaa agttctcgaa agctttcaac accggtgccg
    14581 ttatgatggc tatatgcacc atggatggcg gaacatacct ggatgttaat gaagctttcc
    14641 tccaaaaaac aggacttaaa cgcgatgagg tgattggcca atccctacac gatcaaaaaa
    14701 tatactctga tgtaaatagg cttaaccagc tcttggcaca gatcagggaa aagggtaagg
    14761 taaacaatgt ggaagttggc attagtaacc aaaggggcga ggagttaacc ctgctgcttt
    14821 cggccgaggt tatcagcatt caggaaaatg actgcatcat gattgccatg aacgacatca
    14881 ccgagcgcaa gcggatggag gaggaaataa ccaacagcag gatacagcta aagggaatac
    14941 tcgataacct gccattcatt gcctggctcg ccgacaacaa aggtcgctac ctcctggtaa
    15001 acaaatcttt taccagtttc tttaacaccg acgagggtag ggttctcggg aaaaaacatg
    15061 gcgatttctg ggctaaagcc cactcagcca atctccgaaa gcatgaggtg gaagcagcta
    15121 aagctaaaaa atcattatcg tgggaggctt ttgaaaaaaa cgacctgcag gaagagtggt
    15181 gggagcactt tgttaccccc gttattaacg cggagaatga agtgattgcc actactggct
    15241 tagcccgaat aattaccgat caaaagctga ttaggctgca aattgagcaa aatctggaac
    15301 gacaaaaatt cctaaccgaa atttcatacc tgttcaacac cggcgtaaag ttcgagacca
    15361 aattaaacca atccctgcaa aaggtttccg ggttgctaaa actcaaacgg gcttttattg
    15421 ttgaacgtat tcattcaacc acgccttccc caaaggtttt cgagtggcac gacagcacag
    15481 ccaacttaga ccaggatgcg ctgattagct cacttgataa gtattctcct gcaattttcg
    15541 accatttaaa ccaaaccagc caactggtag ctgacatttg ctccaccgaa gaaccgtcgg
    15601 tgaattcgct tagcaaagcc ctgtctgcaa aaaatctatt catctatccg ctcatcagcg
    15661 agggttgctg tataggtttt ataggagttg agtactccag ctacaaccct cagactaccg
    15721 gcaacaacag ggagttcatg cttacgtttt catcaatact aaccgcagca cttgaggggc
    15781 aaagcaagga gagcaagctt ctggagagcg aacagcgttt ccggacattc tccgagctgc
    15841 tgcccgaaat ggttttcgaa gctaacgaca ggcaggtaat agtttttacc aacaacaacc
    15901 ttcttgaagc attcggattc agcaagcgga aactgccggc tagcctcaaa attcacgacc
    15961 tatttatggc ctccgagcat gacaagctaa caaaccacct ggccaaatcg gcccaaaatg
    16021 ggcagcagca gtcggccgag ctgcttgctc aacgccgtga taaaaccacc ttccccaccc
    16081 taacccatgt tcgacgagta aagctcagca aaagcaagta caggtatatt ggcgtgatgg
    16141 ttgacataac catgcaaaag caacaggaag ccgaactgct taaggctaag gagcaagctg
    16201 aagcggcagg ccgtgccaag gagcggtttc tttcgaccat gagtcatgag atacgaacac
    16261 ccatgaacgc cattattggg atgaccaaca tcctgctgga ggaaaatcct gcaaccagcc
    16321 agctggtaaa cctgaacgcc cttaaatttt ctgccgaaaa cctccttacc cttttaaacg
    16381 acatactgga tttcagcaag atagaggcag aacgggtaga tctccttata aatgaaaccg
    16441 acattcactc gcttcacgcc ggaatattga gctcttatgc gcagctggcg gctcaaaagg
    16501 gcattaagct aaccggtagc atcagcccct taataccgca taagctttta gccgataggg
    16561 tacgccttaa ccaaatactc accaacctgg ttggtaacgc cattaagttc acctccaatg
    16621 gaggcgttga cttaagtatt tcgcttcaac ggcagcttaa aaaatcggtt tgggttaagt
    16681 ttacagtaac cgatacgggc attggaatac ctaaggataa gcagaagaaa atttttaccg
    16741 agttcaccca ggttcacgac cagggaggca agtacggtgg cacagggcta ggccttgcca
    16801 tatcaaaccg tttggtaaat cttatgggcg ggcaaatcag gctaaaaagc gtattcggta
    16861 agggttctac tttctacttc agcataaaac ttgatttgga accctaccat gaaacgccag
    16921 agcccgctga agcacccgaa atcaagcttg accccaccag aagctacctg gttcttgtgg
    16981 tggaggataa cgagttgaac acccttattg ttcagcgatt tcttaccgga tggggaatta
    17041 acaaccacca cgctgcgaat ggagtggttg cgctcgaaat ggctaaaaat aaccgctacg
    17101 acctggtgct tatggacctg gaaatgcccg aaatgaacgg ctatgaggct acaaaagcct
    17161 tgcgaaggtt aaaaggaaaa accaacaatt cggttcccat aatcgccctg agcgcatcgg
    17221 caatgctcga tgtgaaacga aaaattttca gcatcggcat gaacgatttt attctaaaac
    17281 ccttcaaacc cgccgatttg aaaaaaaagg ttctatttta tctttctaaa gtgtaggatt
    17341 tgtatgcgaa ggtttattgt tttctttgca gctgtttcta gcctatcatt tttttccctc
    17401 gaaaaaaatg cggcacaggt taccatagag cttagggtaa aagaatcaat aggctctact
    17461 tccaaactgt cggtttatac tggtaaagaa tatgttaccg ttgattcatg ccgccccacc
    17521 attgagggga tttaccgttt caacctgcca gaaacggctc ctcttgggct ctacaaacta
    17581 accgtaggca aatcggggtc atttgaccta atcgtttcgg gcgaaaagca tgtagcgctc
    17641 caaacggttg cctttgccgt tgaggatagc ctaagggtta ttgactcgga tgaaaacaaa
    17701 gttttttttg attacatcaa actcaagaaa acatccgaac aacagcattg gcttattaaa
    17761 tcgctgctac aataccacac ttccggtgat gatgtttatg aaaagttggt tgacgaacag
    17821 cgtcgcctaa cggattcttt tgatagcagg gcgtcggagc tggcgcaagc gtacccatct
    17881 ctattcgttt caagctacat caacctggat actaagctgc aagctacagg aacaaaaagt
    17941 gattgcaaca gcagaaggat gaaagccgat atatggtgga atggggttaa cctgctggat
    18001 aacaggctgc tgaacacacc tatacttatt ccccggctct ggagctacat tgaaagcctg
    18061 ctttgcgagg atgaactcgg tatggaggag caggattcca tttttgtgca ctacatccaa
    18121 aagttattcg cccttcccct ttcggaggaa atccgtaaca agtttgccga aacactagcc
    18181 gacggatttg ccgaaaccga ctacttcgaa gtaatagact acctagcgca cgctggcggt
    18241 aaagcagccg caacggtttt tgataatccc gattttaaaa caaggctgga acttgagcgt
    18301 cccctgatgg tagggagtaa agctcacgat tttacagcta agctcatgga aggtgaaaaa
    18361 tttaagctgt ctaggagtgt atcccggtat gaacttattg ttttctggtc ggtttggtgt
    18421 ccccactgcg ttgaaaccct gcccaaaatc agaagtatct atgaagagtt caggcataaa
    18481 ggatttgacg tggtggctgt taacattgat gaggaacagg agccatggaa ggagtttaca
    18541 agcaacaaca gtttgcaatg gcacaacacc caggtgcctt ttagctccaa cagccccata
    18601 ccggagtact acaacgttga cggaacgcca aaaatgtttc tggttgacaa ctcgctccgc
    18661 atactgtcgc gacccacgtc ggctgcccag ctacatgcca cgttacgaaa actttacggt
    18721 gaataacaat agcaggcaat atcctgtaaa cagcagtagc aaccctgcta tctgtttata
    18781 aaaccaatat atttggctaa atctccccga agggcgtcaa cccgaaccgg ctactccagg
    18841 taggtgtaac cataaaggcc tgagcggtag ttcgatagaa actccttacc ctccttggca
    18901 gaaatgatac cggctttcac cgacgtagta acccaggttt cgagcgttcg caccattttc
    18961 ttggggttat attgaacgta ctccagcacc tcggccaccg tctcgccatc aataatttga
    19021 tcaatctcgt agcccttatc gttaaccgat acgtggacag cattggtatc gccaaaaagg
    19081 ttgtgcagat cacccagaat ttcctggtat gcgccaacca aaaatgcacc tatatagtat
    19141 gactcattag gttttacggt atgaaccggc aggtagtagg aaatattacg ggtagaaata
    19201 aagttgtcaa tcttaccgtc ggaatcgcat gtaacatcct gaagggtagc agcccgatcg
    19261 ggcttttcgt taagtcgctg aatgggcata atcgggaata tttggtcaat agcccaggtg
    19321 tcgggaaggg actggaaaag ggaaaagttg caaaagtatt tatcggccaa caacttggga
    19381 agcgaccgga actcgtcggg aacgtgctta atgtcggcag tcatctggtt gatatcccgg
    19441 gcaatagacc agaatagcct ctccacctgt gcccgggttt ttaggtcgag cagccccagg
    19501 ctaaaaaggt cgagggcctc ttcccttatt tgctgggcat cgtgccagct ctccagcatt
    19561 ttagactggt ttagcttgtc ccagatttcg tatagctcgc taaccagctc gtgcgggttt
    19621 tcgccaatat cctcgttctc ctcccagctt ggtagcgagg cggtttcaag aacatcgaag
    19681 atgagaaccg agtgatgggc ggttagcgac cgtcccgatt cggttataat gttgggatgg
    19741 ggaatgttat tcttatcgct gacatcaact atggtggata cggaatcgtt cacgtactcc
    19801 tgaaggctgt agtttatgct gctttcgctg ttcgatgagc gggttccatc gtagtcaacc
    19861 ccaaggcccc caccaatatc cacaaattcg atgttgaaac cctgaagata cagctgcaca
    19921 taaaactggg aggcttcccg aagggcaagt ttaatgcgac gaatcttggt aacctggcta
    19981 ccaatgtgga aatgaactag cttgagggaa tctttcatac cgttaccttc cagaaactcc
    20041 aacgcctgaa tcagctcact ggaggtaagc ccaaacttgc tcacatcgcc gccggagtcc
    20101 tcccatttac cgctccctac gcttgccagc ttaatccgaa ttcccaggtt gggcttaatc
    20161 tttaaacgtt gcgaaaccct agccaccagc ttaagctcgt tttgcttttc aatcacgatg
    20221 aagatctttt tccccatctt ctgggccagc agggccagct caatgtagtc ctcgtcctta
    20281 tagccgttgc aaattataag cgactcattg tcgggattgc tggcaataac ggcatgaagc
    20341 tcgggttttg aaccagcctc gagcccaatg ttaaacttct tgccatggtt gattatctcc
    20401 tcaaccaccg gccgcatctg gttcaccttt atggggtaga tgatgaagtt ttgccctttg
    20461 taaccatatt cctgggcagc cacctcaaac gagcgggaca tatcctcaat tcggttgtcc
    20521 agaatatcgg tgaaccggat gagcattggc gcagaaacat cccgaacctg cagctcatca
    20581 accaactcgc gcagatcaat ctctacccca ttattcttag gtgttaccac tacattgccg
    20641 ttactgttga ttgaaaagta attcaccccc cagccgttta tgttgtaaag ctcggctgaa
    20701 tcctcaatgc gccattttct cattccgggt tataatgtta tgtggttatg aatcaacgga
    20761 ttaataaatg attttatggg gctaaattag ccttaaaatc ttactataca aagggaaagc
    20821 tgaaatttat ttagtgcaac acaagcatat tacccctacc aacatgaatt gcggggtgaa
    20881 aaatgaataa ccactagtag cacaaaacgg ctactagcaa aaaatgaacg ggatgtacaa
    20941 gtgcattggc cagcttgtgg ttgttcaatg aaataaggaa agcacagagg gatccattta
    21001 ggccatagct atacgcctgt gtatgccctg gcttgtggaa agtagtatag tcaaaatgta
    21061 acccggcacg aaagtccaag tgctgaatgg gtatcagcgg ttgcggtaag ggcagggtta
    21121 acaattgcag gctcaaccct aaagctcagg ttgtcgtcaa cattccaatc gtgaaggaat
    21181 ccatccacgt tggcgtccag aatgttgatg atgtagatgg cggtaaggcc aagaatgctg
    21241 agatccctat tccggcggta gctatccata tagtatcgca gctcatcagc agttctagca
    21301 ccaccaaatt catccaccgt ggtgtcatca ccatcaatat tcagctttaa agcggtttta
    21361 aagcggctgt agtacctgtc attccagctg acgaggtagt aaagtgtcgc caagccgccg
    21421 taaactacag gaattttcca gtacttttgg ttgtaggctt gccccattcc cggtaccaga
    21481 gtggaaagaa tggtagcagc tgcaggtgag tgcttgctat tgttgtaaac cagcacggca
    21541 tccatggctc ctgccacata gaatgccccg gcagcggcaa tatagaggtt gcgggtttgc
    21601 ctttgcttca ccattcgctc tttatataat tcacggttgg ggtctgtgga ggtcaaacta
    21661 ttataggccg aaagcctgct taggtaggtt tggttggcct ttaccccaag gtaaatcatg
    21721 ctgcccatgc ctccatatag aaccggtagc ttccagtact ggcggttagc aatttgcccg
    21781 taaccgggca gcagctgtga gccaagccag atgcgggtgg taaaatcttt agcagggatc
    21841 gccggcttaa tattttcctg agcccctgct actttgccaa tacatgtaag ggctaaaaac
    21901 agaatagccg acctagtcaa gcgagcatgt cggccatgaa tcatggaaat agcctttgtt
    21961 ctcaacgttg caatattatt tccgtcttgc tgctttctgc ctttactctt caattttggt
    22021 aaacttatca ataataacgc taagctcatc agggttgttg taggaaatga caattttgcc
    22081 atcgcccttt ccgcctggct ttacatctgc acgtagtccg agcctgttgg tgaggtgttc
    22141 cctcaaagcc agctcaggcc cggcaaggtc gggcatgact ttagacctgg actgcttgat
    22201 ttggggttca gaaacggtat ttctaaccag ctcctcggtt tgacgaaccg acagatcgtc
    22261 agaaataacc ttcttgtaaa gcttgagctg aagcgaggca tcgtcaatgc ttatgagagc
    22321 acgggcatgg cccatgctga ttattgagtt gatgataccc agctggattt cggcaggaag
    22381 cttgagcagc ctgaggtagt tggatatggt tgcacgtttt ttaccaaccc tttccgaaag
    22441 ggcatcctgg gttagactgc attcatcaat gaggcgctgg tagctaatgg ccacctcaat
    22501 agcgttgagg tcttcgcgtt gaatgttctc aacgagcgac atttcgagca ttccctgatc
    22561 atcagcagtt ctgataaagg caggaagggt tttcaaaccg gcaagtttgg cagctctaaa
    22621 gcgtcgttct cccgaaataa gctggtaccg tccatccaac aagcgaactg taataggttg
    22681 gattattccg agtttggaaa tggactcggc cagctcgctc agggattcat catcaaaatt
    22741 ggttctcggt tgaaaagggt ttgggtcaat cagctcaatg tcaacctcct ggacgagttc
    22801 ggagatagca tgccttggtg tctgtacatc gctgtttcgg gtcgattcga aagttgaatc
    22861 ctcaataagc gctcccaatc cccttcccaa tgccattttt ttagtcatag ttttatctcc
    22921 cagcctatgc ggtaacggtt aatcctgggc aatcttttgc tcattctcaa tctgagtctg
    22981 gttgtttttc tgaagcagct ccctggccag gttgatgtag tttgccgagc ctgtagaggc
    23041 cgcatcgtag agtattacag gtttaccata actgggggct tcgctaagct tgatgttccg
    23101 ctggataatg gtgtcaaaaa ccatttgctg gaagtgcttg cgaacctcct caactacctg
    23161 attcgataat cttaacctcg ggtcgtacat ggtaagcagg aatccctcga tttgtagctc
    23221 agggttcagg cgggattgaa tgatcttaat ggtgttcagc agtttgccta gaccctcaag
    23281 ggcaaaatac tcgcactgca caggaatcat caccgaatca gcggcagtaa gggcattaac
    23341 ggtgattaga cctagtgatg gtgaacaatc tataaatata aaatcatagc tatcctttac
    23401 cttggcaacc acctctttta atattttttc acggttcggt aggttaagca tctcaatttc
    23461 agcccctacc aggtcaatat gagatggtat caggtccaaa cccttgattt cgctgttcaa
    23521 gatgatatcg ttggggttta cctcatcaat caggcattcg taaatgctgg ttttgatgtt
    23581 tctaatatca aacccggttc ctgaagtggc attggcctgt gggtcggcgt cgataagcag
    23641 aacctttttt tcgagcactg ccagacttgc cgacaaattg atagcgctgg tggttttacc
    23701 caccccaccc ttctggttgg ctattgcaat aacttttccc attacctaat ctttaacatt
    23761 aaaacaagcg tcaaaaatac aaaacctccc gcaaccatcc tgcccccacc gctggggaat
    23821 atgaatagta ttcaacagga tataaacatg ttttcaacaa ccgatttaat catacttacc
    23881 ggcaaagata gaaatttttc aaaaaaaagg ttgaccctgc cttaagccat acaattaaac
    23941 ttctatgtgc tatctttgca gctggaattc gatgctaaaa tctatctgat tggtaaacag
    24001 ctggtttgtt attgtaaacc cccgggcagg tgggggtaag ggtgaggttg actggccgct
    24061 catcaagggt ttgttgacta aggaggggat agcttttgga tttgctctta ccgaacataa
    24121 gcaccatgct gtggagctta cggtgaaagc catcaatagc ggctacaggc agctgattgc
    24181 cgttgggggt gatggaacgc tcaacgaggt ggtaaacggc attttcatcc agcagtcggt
    24241 tccacctgcc gagattctgg ttggagtaat tggcgtagga accggcaatg actggtttcg
    24301 aatgtacgaa attccataca ggtacgccga tagcgtaaga gcaataaaaa atcgtaaggt
    24361 tttctaccag gacgtaggtg tggtggagta ctatgagtct agcgtaaagc ataagcgata
    24421 ctttgttaac accgccggaa taggctttga tgctgaagtg gcgctccgaa ccaacaggtt
    24481 aaaggatttg ggaagacatg gttttttgct ctacctcatc agcatccttc gagctctttt
    24541 tagctacaaa acaacccgga taaacgtaaa tgtggatggc cgccatttac aggataaaat
    24601 tttcagcatt accctgggga tttgccgtta caacggcgcc ggtatgctac aggttccctt
    24661 tgccatctcc gatgatggaa tgcttgacgt taccgttatc cgcagaatca gcaagctgga
    24721 tgctctaacc agcattcccc ggctctacaa cgggaaaatc ttaaaacact ccagggtaac
    24781 cggactgcgt ggccaaatag ttgaaattat gagcattccc tcaactaatc ttgaggcaga
    24841 tggcgaatcg ctaggcgaaa cccctctgcg cttcaccgtc ctaacccgtg caataggcgt
    24901 ggtggttgac gaaaactttg atgctaagga taaccgccct gctgttattc aggagaaaca
    24961 aaccctctcg gcagtctaat catcctgctt cccgcttatt agagtttcca ttcacccgca
    25021 catctaccct gggcattact ttttcaacat ttatatagca gccaatacta gacaaccccc
    25081 tagattcgtt tcttaccagc aggcatcata ccgaaaaaac atgccggggt aaccattaag
    25141 atcaaagctc aaaaaaatca tcattaacat ctaaatgaac ggtatgttgg aacctcctcg
    25201 taacaggttt tactttggtt acgtctgtgc ttttgaagca tggaggtttc atattaattt
    25261 gatttgttta cttttacgag gaaaaatcat aatccatgtt ccagaagtgt ttcataacag
    25321 tattggtttt gctcagcagt ctttgtgggt ttagccaggt tggaggattt ggagcctacc
    25381 agttcttgaa ccttccattc tcggttcgtt ctgctgcact gggaggcaag gtggcaacta
    25441 ttgacgaacc ggacatggcc atggtgatgc ttaaccctgc cctgctggat tcaacgcttc
    25501 acaataacct ttacctttcc tactcacgct acgttgacga tataagctat ggtgcatttg
    25561 cctatggcag gagcagcaga tgggggacat ttggggttaa catccagcag gttagctatg
    25621 gatcattcat agaggccgat gaaaccggat tggttcaggg aacatttaag gccaacgagt
    25681 tagccgtgtc gctaatgtat gctaggacac tcgatagcct tttcagtgtt ggcatcagcg
    25741 taaaaccggt atattcgcat ctggagcgtt acacctcatt tgggcttgca gtggatattg
    25801 gtggcaacta ccactcgcgt aacgggctgt tttcgtcggg agtagtgttg aaaaatattg
    25861 ggctaatgct taagggttat acccggggta acccggaaac cttgcctttt gaaattgtag
    25921 ccggagtaag ccaaaagctg gagcatgccc ccttccgttt tctggttaca ttccatcagc
    25981 tgcaaaacat gaacatctac tacgaatcaa agctgcagga tagtgaaaca agcctgggcg
    26041 aaaatagctc acaaccagga tttgccgaaa gggcaggaag ggaatttctt agccatttga
    26101 taatcggaat ggagtttgtt ccggttaaaa gcttttacct aagggcggga tacaactacc
    26161 agcgccgaaa tgagctgaag gtgcccgaga aagtatccat ggttggcttc agctggggat
    26221 ttggtgtaaa agttgcaaaa cttcatatca gctacagccg tgccacctac catttggcag
    26281 gatcaaccaa ccagttctca atatgcacca acctcaacaa ttttgttagc acaaagccaa
    26341 tttagccgaa tagacttaac tcttttttta taataacttt gctccggatt ttctagcagt
    26401 cctaggctgc ttccgcaaca catctatact aacgcgataa tctggatggg taaaaaaata
    26461 acaattgcca ttgatggctt ctcgtcgtgc gggaaaagca cctttgcaaa aaaaattgcc
    26521 gagcgtttgg gatacctcta cattgacagt ggagcaatgt accgggcagt aaccctagaa
    26581 tttcttaatt gggaagcatt ccgcaacaac acacccgaca ccagcatgac ccaacaaatc
    26641 ctgtcgggat tacaggtggg atttagcttc aatgagcaga aaaaaaccta cgaaaccacc
    26701 ctaaacggaa aggtggtgga ggatgccatt cgcagcccac gcatttcgga gttggttagc
    26761 gccgtatcgg ccattcccga ggtaagggaa aagatggttg agctgcaacg ccttatgggc
    26821 aaggataaag gcgttgtaat ggatggtcgt gatatcggaa ccgtggtgtt tcccgatgcc
    26881 gagttgaaga tttttatgac cgccgatcct gcaatcaggg cagaacgtcg gtataaagag
    26941 ctgcaatcaa agggagtaca ggccagcttg gaggaggtgc ttaataacat attggaaaga
    27001 gacaagcttg accaaacccg cgccatttct ccactccgga aggctgaaga cgccatagaa
    27061 cttgacaata gctacatgac catagacgag cagatggagt gggccgaaaa gttgattgcc
    27121 gcaagaatct gctgaaccaa actcccgaaa ggttgttaca tatttggtat gaggttgacg
    27181 gttgccggca agcaaaaaga atgttcacta tgcgcaaacc ggatttaaat ccataagcgc
    27241 tactttaaaa tcagcattga attggtacct ccatgcaagt tgatatagat cagaaatcgg
    27301 gattttgctt tggtgttgta aacgccataa accaagctga agtcgggctt aagcagaatg
    27361 aggagtttta ctgcctgggc agcatcgttc acaacgagca ggaaatttca cggctcgaaa
    27421 aaaaagggct aagggttgta tcccgagacg agttctccgg cctttcgggt aaaaaggtgc
    27481 tggtgcgtgc ccacggcgaa ccaccgtcaa cctacgagct ggcaaaagaa aacgacgttg
    27541 agctcattga tgctacctgt ccggttgttc tcaagctgca ggaacgtgta aggaaagcgt
    27601 gggagcaaat gaaaatagcc ggcggtacgg taattatcta tgggaaaaaa ggccatgccg
    27661 aggttatcgg gctagtgggc caaaccgatg ggaatgccgt agtggtggaa tcgccaaacg
    27721 atttaaccgg aattgatttc tccaaaccgg tagagatatt ctcgcagacc actaaggatg
    27781 cggaaatgtt taagcagata gtggacgtaa ttaaggaaaa aatgcaatcc tgctttctgt
    27841 cctcaaacat acccataaag gttaaccata cgttttgcgg acaggtagcc aaccgaaaaa
    27901 aggagctaat cggatttgcc gcaactcacg atgttatcat ctttgttgga ggtcaaacga
    27961 gctccaatgg taaaatgctt tttcaaacct gtaggtcaag caatccaaaa tcatactatg
    28021 tttcctccgc caacgagctt cggcccgaat ggtttgctgg agcaaacagc gtgggcgtta
    28081 gcggtgcaac atctactccc ttatggctga tggagcaggt ggcacaggaa attgaacgca
    28141 tcaccatgcc ctaaattcct catattctgc ctaccattcc ttcaagtaaa tcatatggca
    28201 gataattttc cgctcaataa cctacaaaag agcttattat gatgctctgg caataatttt
    28261 tttgcttacg tttgcaacat gaaaagggaa gttcgcatag tcggcaccat ttttcttgtt
    28321 gctctttact gctttgcagc aagtgtggtt tgcatcccta aacttgttcc ccttgaagag
    28381 ccacataatg ggccaggcta cttttttaac gcctcatcaa atctgctttc cctaactcta
    28441 ccttcaaaaa atccggcagg cagcattggc cttagcccca tctgggtttt aaaaaatcag
    28501 ctaaaagact tttcctatac tataaaatcc gttgagctgt tcctgtccac catatttaca
    28561 cagtaccgaa atgtttccat tagctaccca ataaggctcc gaaaaaccga tctgctttac
    28621 ccatttcaat acttctggta agcgcattcc aacttaacca cattgccgct ttcggcaact
    28681 cctaaaggtt ttttgcaggt attgtgcctc aacaaaacct gaataactat ttttcaactt
    28741 atctaaatct acaaaaatga atacaggaat agctattata ggtgttttaa tagtggttat
    28801 cagcatcata ccatttattc tcgtaagcag ggcgaaaagg aaaaaggaaa aaaggctgcg
    28861 cagtgagatt gccgggatgg ccaaaagcca tggctgcagc atttccatgc tagatgttct
    28921 gaaagactat gctgttggag ctgacgaaaa caagcatttc atattcttct acaagcacca
    28981 gaatggaaat gaaaccaagc tggctattaa cctggccgac attcagagct gccgggtaat
    29041 aaattcaacg cgcacaattg gtggaaggga cagccagtac gtcattaccg acaaggtggc
    29101 tttatgtctt actccggtgg agaaatccaa ccccgagatt catcttgaac tctatagtgc
    29161 cgacgacggc acccagctgg taggagagct ggagctggtt gaaaaatggt cgaaagctat
    29221 caacaaccag cttaaaagca tgaaacccat caagtaaacc aataacttct ttacacctta
    29281 ccattgaaaa gcccttcatg cacgattgaa gggctttttt cgacctacct caaccacttt
    29341 ttaaattgac cttgaaatga gcttatctaa tgtttactga atcagcgtta gggttatttt
    29401 ctcaaaactc atcaatctac ttgtaattat ccgttgaaaa tcatgcaatt aggatggaca
    29461 tttttctatg gcaccactag acttgcgata tttagcccat tgtgggtatt ggtttcttta
    29521 cctaaccatt ttcaatagaa tcttaaatca gcatgcacct tggctggtta cgtgccgatt
    29581 atacgaacta cggaattatg caaatatgaa actggaataa attttcacag taaaaaaata
    29641 ttcggatttt acttacttgc ataagccatt tgttaccgaa gtttgttaat ttgtaaacga
    29701 aaaacaacag ccagatagaa gcaaatgaac tactgtaaaa atctctatag ctactccagg
    29761 cgtgaaactc aggaagttaa tattggcggg gtgccaatag gtggttcgaa tcccattcgt
    29821 attcaaacca tgaccaccac caacaccaac gacatcttgg caaccgttga gcagtgcatt
    29881 aaagccgcca aggcaggcgc tgattatgtc aggattacaa ctcagggggt taaggaagcc
    29941 accagcctta gcagcataaa gcaggaactg ctaaaccgtg gtttcacaac ccctataatt
    30001 gccgatatac acttcaaccc aaatgcggca atggaggcgg ccaagtatgc cgacaaggtt
    30061 aggataaacc cgggtaactt tgttacttcc catgcaaagc caactggagg gagtggtaaa
    30121 cagcaagaag aagcagacaa tactgccttg atagaagcta agctggtccc gttgttggag
    30181 ctttgccgag aacgaggtgt tgcactaaga ataggggtca accatggttc tctatccgaa
    30241 agaattatgg ccaagtacgg tgatacccct gaaggaatgg tggaatcagc cttggagttc
    30301 atcaaaatat gcgccagaca caattttcga gacatagtgg tttccatgaa ggcaagcaac
    30361 gtgagggtca tggttcaggc cgtaaggtta ctggcagcca ggatgctcaa cgaggggatg
    30421 gagtacccca ttcaccttgg cgtaaccgag gccggtaatg gtgaagatgg ccgtattaaa
    30481 tcggctgttg gcattgggac ccttcttgcc gatggaattg gcgacaccgt tagagtatcg
    30541 cttaccgagg atcctgaaaa cgagattccc gtggctaaaa agcttgtttc ctacttcgaa
    30601 aatcgatcct cctttggcag aattcccagc atggaccccc tcccctacaa cccattcagc
    30661 tacacccgaa ggaaaaccat agctgttggt agcatcgggg ccgataatcc tcccgttgta
    30721 gtggccaacc tctcgttagc cacaaaaata accggtaaac ttttatcggc atggggatgg
    30781 acatacaacc atgcagcaaa ccaatgggaa cattcagaac ttgctgccga ctacttctac
    30841 attggctcaa caaccattga cgaagaagtc attactgatg gcttaccgtt actttccact
    30901 gccaactcta gtagccaaat acttcgtcag ggaaacaaac ccgcctcctt taacatttcg
    30961 gcgcaaccca tatgccaaat ggttaccgat gccgatgtgc tttcaggcca cctattctcc
    31021 actgttatgg ttagtcccaa tactgtatta attctgagaa ctggctactc caatggtttt
    31081 gcaggacaaa gggcagcttt cataaggtta atgaacaacc atgagaacat tccggtgata
    31141 attagtcggg agtacgatga tgacgatagg gaatcctttc aacttaaagc ctcagcagat
    31201 atcggcggac tgctggttga tgggttgggc gatggggtta tggttaccgc accggcaatt
    31261 gccgatgagg atatttgctc aaccgttttt tccgtgcttc aagctgcccg tacacgaata
    31321 accaaaaccg attatatagc ctgccctggt tgtggacgaa ctctcttcaa cctgcagcaa
    31381 acgctcgaaa aagtaaaggc tcacaccggc caccttaggg gacttaaaat tgcagtaatg
    31441 ggctgcatag tgaatggacc gggcgaaatg gccgatgccg actatggata tgttggagct
    31501 ggtcccggta gagttacact ctacaagggt aaacaggtta tgaagaaaaa tatacccgag
    31561 gaggatgccc ttgatgagct aatcggcctg attaatgccg acaggggttt gtaacttttt
    31621 tcggctttac tcaaccccag ggcaactcac aaatggccag attttcttgt ttaagcccat
    31681 tcgggttttg tacggctttg tacgagaagc tataagccta agtaagctgt tgaaattgcc
    31741 aaatatcatg ggaaaagcaa ccgtgaatgg ggtatcgaaa acacccaatc atccgggtaa
    31801 aactcaaacc attcctaggc tattctttcc atctcgtttt cgggatttaa ctcccgcatg
    31861 gagttgccgt tgtttttccc cctggcaagc gatgcccaca ccccaacaat gcatagcaaa
    31921 acaaaaactg taaaggttgt ccccaccgac cagccaaact gcctgtagtt tcctgggttg
    31981 atgtccgact tgccaatgta aagttgaagt ataagggtgg caatgcccat gcttgccatc
    32041 tggccggtta acctcatggt ggcaactgtc cccgaagcaa ttcccagaaa cttcttttct
    32101 acagaaccca taacagagtt ggtgttgggt gatgaaaaaa gaccaaagcc gaatcccagt
    32161 atggcttgtg caacaattag gtaggtaatg ctggtattct taccaattgg aattagcaag
    32221 gccagcccta cggctattat gcccattcct aatgatgcca gaattcgcgg atcgtgctta
    32281 tcggacattt ttccagacca cgacgataca aatgccatta gtagcggctg aacaattagc
    32341 agcaatcccg cctcgcgagg agatagaccc ttaatatact gaaggtaaag gctgagcaca
    32401 aatgtaatgg caaatgtggt tgcatagttg attagtgcgg ctacatttga catggcaaac
    32461 aagcggttgt tccgaaaaag tctgacgttg aatacagggt agctagcccg aagctcaact
    32521 ctaacaaaga aaataagccc gataaagcct agaatggtca ggcaaactgc aacagggtcc
    32581 ggaagttgtg aaaagccata catgaaaagg gtcatggagg gaacgtagat tgcagccccc
    32641 gcaaagtcga aattctcacc cctggcatca gcccactccg atcgaactgc catccttatg
    32701 gaaagcgctg aaaaaattcc cagaaaagcc ggtacataaa ataaactttc ccatccaaac
    32761 caggaaatta gtaagccacc aattaggggt gccgccgata gcccaatata caccgaggta
    32821 acattcaacc caatagcctt tccacggtcc gatgggggaa aaaccgaggt aattaatgcc
    32881 acccctgtag caaaaaccat ggcgcttcct aacccctgaa cagccctaaa agttatgagc
    32941 acggcacctg atggggagat agcgcacagc accgaactaa ttgccatgat tatattgccc
    33001 caaaaaaata cacgttttcg ccccgcaata tcggctatct tacctatcgg aacaagaaac
    33061 accgcagagg agagcaaaaa tgccatagta acccagctca tccccactgc gttcaaacca
    33121 aaatccttac cgataagcgg tagagccaga ttaaccgaag caccaataaa cggcgtaata
    33181 aacgacgata ccataaccaa cgccagaact aaacccctac taaccttctg ttgctgcatc
    33241 ttaaaaaatt ttcgcaaagg tattcgtttt agtcctcttc aaagtagtgt ttattttttt
    33301 ttagctgaaa aaccagctat tttagcgttg gattagatct tcacgactta tggctaagat
    33361 tctggtagtg gacgatgact caacattctg cctgatgctc aagacctttc taaccaagca
    33421 tgagcatagc gtggtggaag cattttcgtt tgacgaggct gttaggcaaa ttcgctcgga
    33481 gaattttgat gtcgtcctga ccgacttccg ccttccagag aaaagcggaa tggatgtgct
    33541 ttccgaggcc aagcataaat catccgccac agttgtaatc atgatgaccg ggtatgccga
    33601 tattcgcatg gcggttaacg ccataaagca cggtgcttac gactatgtta ccaaacctat
    33661 aaatcccgat gagattctta cctccattag cagggctcta ccgggcaaca ggaaagccat
    33721 tgacaaggac ggcactcaca actttatatt tataaatggc aatagcgagc atagccagga
    33781 ggtaagccgg tatataagca ttgtggctcc aacaaacctt tccgttatta ttcagggtga
    33841 tagcggaacc ggaaaggagt acatagcacg taagattcat gccgagagca agcgtagcag
    33901 caaagctttt gttccgattg actgcggggc tctcccgcgc gagcttgccg ccagcgagtt
    33961 ttttggacac ctgaaaggct ccttcaccgg cgccattgcc gataaaatag gtcagtttga
    34021 ggtagccaat ggtggaaccc ttttcctcga cgaaatcgga aacctaccct acgacatcca
    34081 ggttaatctt cttagggcca tccaggaacg aaaaattaag cgtattggga gcagcaagga
    34141 aatccccgtg gatgttagaa taattgttgc ctccaacgaa aaccttctaa atatggtgaa
    34201 ctccggagca ttcagggagg acttgtacca ccggttaaac gagttcacca ttcgggttgc
    34261 ccccctgcat gagcgaaaag acgacctgct aatttttgcc aagcattttc ttagccttgc
    34321 caacgttgag ctgggaaaag gtatcaacgg gtttgacgcc gatgttctgg atattctaat
    34381 ggactacacc tggccaggaa atctaaggga gcttaagaat gtgattcgta gggctgtgct
    34441 gatggagcag tcgaacttgc taaccgtttc aagtcttccc gacgaaatcg tacatcgttc
    34501 cggtcctggt gatgatgata aatcggccaa gcacgttgaa aacctccgta acataaagga
    34561 aaaagccgag tatgatttga ttatatcaac cctcgaaaag gctaagtaca acaagagcaa
    34621 ggctgcccta ttgctaaata ttgaccgtaa aaccctgtac aacaagatga agcattacgg
    34681 aatttctggc taaaaaaatg atatagtaaa aaatgctgcc attcagtctg cctcctgttg
    34741 gcacatttca acattctttc tagttcagca tggccagtat tcccactttc ctatcgtttc
    34801 gttcaaccga aaaagactta ctttgctttt caatccgctc aattagggca agcgccttac
    34861 atagcagatc cttccgtaaa acgtcattgc tagtaaacaa cccatcgcgg taaagaagct
    34921 ccgaaagcat ttcaaccttt tcaacgtgtc ggaactggtc atcattatca ataagggtta
    34981 tcatttcatc acaacttagc cgatagaaga actgacagga cctgtcaaag taagcgctgt
    35041 aaacttcctg aagctgctta tgaatctcat cagcattatc ctcgttaagc ttaccaagca
    35101 agccgtttat aagcttgaaa aattcctgaa taaccctaag aatgtaatcc cgctccatca
    35161 taaggcctac agcttatccc aaagatttta aggtaaaggt aaaataaaaa atagtagtat
    35221 ctcccccaac gtattggagg ttttacacca tacataataa gatggaatag gtgtgacagg
    35281 ctgatgcaaa ggattcacaa gcatcaaatt atgtttggag aaaggaacgt aaagggttgg
    35341 ttaggagtat ttggtagaac agacagcctg gtaagctgcc tgctttagct gctagtaccc
    35401 taaactaatt tccagcggac ccatcaacca aatcatctac aaatttttta attgatttgg
    35461 aatcggttac accgtaaaag tcgtagtagt actcaatgag atacttttta atgtcatcct
    35521 tgctcatatt ctgccgatga aggatgggca gaattgtaag aaactcgttg gtaagctgca
    35581 ccttatcaac aaaaatcagg ttaaatctgg taatttcctc gctggaatgg gtgttacttt
    35641 catacctgta aacggcaaca tttggaattt cgtcattgga aatagttcct gcgctggtgt
    35701 tgagcagctt atccttttca attttaagca cattctgttc gtatccgatt ttcttggaaa
    35761 ccggattatt gttgaccctg tagtagaaaa caatgaacct gctatcgttc agctggaact
    35821 tgtcaggatt tacggggacg agcacctcgt tgccgatgac cgaaaattta tcgttgccga
    35881 agtagcttcc caagtcttca accacacttt cgttgggcct ataccgttca gccataaggc
    35941 ttctgcgctt agtggggaca gcaacatcct ttacagtggc agtaagctgt tgaatggcat
    36001 caggggcagg aggctcatga agcttaatca tgtagcggcc catattctgg ccaatggaga
    36061 taacgtagga gtcaaagtta tcaaaggtaa ccttatcgct aggttggagt acgtctcccg
    36121 ctctgagaga tgttcccgtt cggtagttaa ccacattccc cttaattcgg gttacaataa
    36181 aactatcctg agcaattgcc gtggtagcca tcataaccag aagaagagct agaatatttg
    36241 ctttagattt catggcgtta ggtttttgtt attgcttttt ttgatttcat caattcctgt
    36301 accaaaatag cgaaatgttg ggatttgcca tccctttaac ttgctccgac aggtgatgca
    36361 aagtattgcc ctattttttg cctgcagcaa ccagcacaaa ggagctatca atccattgca
    36421 tgacaaggtt tctcggtata agggttgtat ccctaagggt tatccagaat tttttgttca
    36481 tgtggtagcc gggttcaaca aaggtatatt tttcctgcag ctcaagggca atttcgggat
    36541 cacacttcaa ggttaggctg ctatcgcccg aaaggcttat catggcgaat attctgccac
    36601 ccacctttag caccaaggtt tcatcgtcga acgggaggct ctcggttgcg ccaggttttg
    36661 aaaggcagta gtcacgaatt tcttcaacat tcataccgtt aaagtgttgg gtaaaaggca
    36721 tcctcgatat gctctatagc tagtgagccg ttggcaataa ccaccgacac aatgtcgaaa
    36781 cgcacctcgg tatcaaggtt gtagtggcaa acgtaagcat cggcagcctc caccagccgc
    36841 ttttgctttt tccttcgaac ggcttctttt ggttcctccc agtagtcgct tgtccgagtt
    36901 ttaacctcca caaccaccag catattccca tctctggcca caatatcaag ctccttgtgc
    36961 ccaaaatgcc aatttcggtg taaaatttta tacccttttg atacgaggta ctccgctgcc
    37021 gaatcctcgc ccagtgtacc taaatccttt cgtttgtcgg tgttaacaat ttgccccatt
    37081 tcgggtgaaa cattatttag cgttatgatg tataaacatt acactttagg ttgaaaacca
    37141 taaaggtcaa aactcactta cttcaacagc taacataaac ctaatgatac ggttgaaaga
    37201 acaaaaatgg ggacatttcc gtcgatttat tctggttata cccatgttgt tgcttttccc
    37261 attcaacctg ctatcaaaca tagacaattt tgagggaaaa atcaagtttt taagcgaggg
    37321 ggtttacgac acgctgagca tggttctcag cgtaaagaat gatttggtca gaatggacca
    37381 gtactccggc aaaaatggtc tggaaatatc ctacctcttc gacctgagaa gcggtaaggt
    37441 ttttgctctt tcaggcaggg aaaaagtgta taaggaaatc tacctgcacg atcagtcaaa
    37501 aaaaagaagg gaaacggacg taaccattat caagagccaa aactcaatgt tgataaatgg
    37561 aacaacctgc taccaatgga gggttaagaa taggcagagc atgaccgaag taacctactg
    37621 ggttgcccaa agtggctttg acttcatgga gccccttcaa cggcttattg gccagctgca
    37681 aacgccattc aacctgttta cgctttgccc cgacatcaag tattttttcc cgctgatggt
    37741 ggttgaccga accatattcc gaaaattcag gtgcagccta agggttgtag atataaaacg
    37801 ggtcgtactc cccgataatt tgttcgctat tccccgtggg tatcgagaat tgcttggcta
    37861 gaactttcgt gaaaggtaag cgaggcaccc tgagagttta ccacaagctg cactttacgc
    37921 cccatgatca atggaaggtt gggttcctga tgacccgccg gaaaaccgaa gcataccggt
    37981 atcctgtact ccttaaccgc ctcactaatt atctggtatg cgtccaaccc gaatgggata
    38041 ttgttgtcct tcatttctga gaaatgaccc accaccaatc cagctataga cttgagcttt
    38101 ccagcccgtt taagctggag catcatgcgg tcaacatggt agaggtactc atcaacatcc
    38161 tcgataaaaa ggatgcgctc ctgtgttgaa gtgtcggcat cggttccaat aaggctggcc
    38221 agtattgaaa ggttacctcc tgttagaaaa cctgtagccg ttccttttcg atttagaggg
    38281 tggtgtggaa tgctatatgc cgggtttctc cccattaaaa cgtccaccat gctgctagcc
    38341 gattcgctat catttatgtt ttttggaaag ttaacaggca tggtagcatg aagggtctca
    38401 atgccaaacc agctggttat aatgctatga agaacagtaa tatcgctata tcccactatc
    38461 catttgggtt taagctggag cttacgcagg tttatcctgt ccaaaattct taccgttccg
    38521 tatcctccac gagcgcaaag aattgccttt acgttcgaat cattaaccat ctccatgaaa
    38581 tcggaagcgc gttcgctatc cgaccctgca aactgaaaat gtttggcaaa gagggagggt
    38641 gccgttttca ctataaaacc ccgtgcagtc agaaaccgga tggccggatt aacatcttcg
    38701 tggctgacaa accttgcagg ggcaataatt ccaacagtat cgccaggttt caggtatggg
    38761 ggagtgatat tctccatgct tcatttaaaa attgaccata aatatacatt ttttatttta
    38821 tctgtttggt tcagcccttt actctttata tgcccttttc ttgaaataaa atcaaacctg
    38881 caggttagcc caactaaatt ttaagtttgg caattaatgt tttacctcta ctgtccctcg
    38941 ggtgaagcaa taaaaaaatc cggcgttaaa ctattgccgg aattaagggt aaagcctttg
    39001 ccacagccca aaagggcaat ccctagtggt ttaccaccaa cttttgcgaa aactgcctgc
    39061 catttgccct gagagctacc aggtaaatgc cgttgggctg attgcgggtt aaccaggtta
    39121 tggtttggtt gccgccacca agttcacctt gcgccagggt ggcaataatt tttccgtttc
    39181 catcgcaaac tattaggttt gccacacaag gcttttcaag atgaaatgat atgctaacct
    39241 cgttcctggc gggatttggc ctgattgggt aaaggtagta gccctcagca tcttgaccga
    39301 tcatatctac tccggtgagc gatgggcttc cggcctcctt ccatgcagtg tacattaact
    39361 cggcaagcgc atgtgaagcg ttggcaaata gcatggtagt aaattcgcca gttttttccc
    39421 aaagggcatt cttatatgcc gttgagctgt agttggacga aatacctgta gcgtagttgt
    39481 cggcagccag cacggaatca acatacttat aatttgcgta aaggtagttc aggatgtact
    39541 gcgtaacgtt atcaatctga tgaatttcag ccccttcgta ctgtgaaagc tgctgttccc
    39601 tggcattcac catggtgctt tcgtaacgcg agtgaatgcc atcgttgccg gttagctgcc
    39661 cgttataatt tttagtaatg tgcattggca tatgtccatc tgcaacatag tggccaaggt
    39721 cggctgcaaa aaacttagcc ctatcccagt ttagtctggc catgttggat ttgagcgagt
    39781 caaaagtggc tttagtggca tagggcaaaa tgccccagtc gtaaacgtta tagctcccat
    39841 gaagagtaat tgcttcgttt agggtttgag ggatgtatcc cgcgttaata aactcggggt
    39901 aattttcaat atcaatgtag tgctttggcg cttcgttggg atcctcatcc tttcgtttat
    39961 cggcatcgga ggcatgttca gccaggtagc tagcccatgt ctgaaattgc tccatttcca
    40021 aattaaaaga tagcgaagat ttgctactga taatgtaatg ccccgtttgt ccccaactgc
    40081 atagaattaa cccaatggct accagtgcaa taagtctagt aaatttattc attttttact
    40141 cagaattaac aacgcaagtt taaacatttt ttaagactgg ttgtttgatg gatttcattt
    40201 taataggttt aacattcatg gatggacgtt ttaactaaaa tatcatctat atttagaaaa
    40261 attatacact cgccgtctta tagtaacacc taatggttcg catcttaaat taacagtagt
    40321 ttaccgtaaa acactggaaa gtgttgcatt agagggcaaa actttttaat attgccaatc
    40381 tttttatgaa gcatcgagcc agatttttta aaagctggat gatgcacctt aaactctacc
    40441 tgttattgag ttaatattct tacttatgat tacatccgta ctatcgcccg aatttgctca
    40501 tctacaacca ttttttcata gtatttgctc taaatttgat actgaaggag aggttatctt
    40561 tagcaaacgt aaccagatta aagttttcaa catagacggt ctaaaaatct cagtaaaatg
    40621 ctttaaagtg cctaatctgt ttaacagatt ggtgtatagt ttttttcgag cttcaaaggc
    40681 cgagcgctcc tataggtatg gtttactcgt agcaagtttg ggcattaata caccgtcacc
    40741 tataggctac atcgaggaaa aacaattcgg aattattcga agaagctact acgtgtgcgc
    40801 ctatcagccc taccgggcaa ccataacagg catacttgga tcaaatgata ctgacagaga
    40861 aacgattatc agggaattta cacggttcac ctttaccaaa cttcaccaac agggtgttct
    40921 ccataaggat tactccggag gtaatattct ggttgaagac ttgggtgagg acagctaccg
    40981 cttttcgata atcgacctga accgcatgaa ctttaaaaag aaggtatcca ccaaacaggg
    41041 gctaaaaaac cttagacggc tcactctcga cattaccgtg aacgatattg ttacaattac
    41101 cagcgagtat gcagctttgg ccggaaacag caaccccaag caaacgctct tctggttttt
    41161 gaaataccat ttagactttg tccggttcaa gaacttcaaa agttggtata aaaaatttaa
    41221 aaggggatga acagctcagc caaaacaagc cgtatagatt ctggttccaa ggccaacatt
    41281 ttatcaattt ccacgctctt agcttccacc tcgtttctat tccctatact agctgaagaa
    41341 tcatagcatc tcgcctctta cttttccggg taatggtatg gctactcagt gccttcaata
    41401 ccaagttcac cgctttaaac cattcgtttt tttaccagcc tcaaagccaa ccgaccaggg
    41461 ctctccttca gtgtaggttt acaattttaa ccagctcatt cgattgctaa taaatttttc
    41521 ttagtcccta cttttgagtt ctgagcactt gacttttaaa atttaaaagc ttgtccagtg
    41581 gttttagccc cgaaggggcg ga
//
LOCUS       DAOSHM010000019        41301 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_734_length_41301_cov_21.177763, whole genome shotgun sequence.
ACCESSION   DAOSHM010000019 DAOSHM010000000
VERSION     DAOSHM010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 41301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 41301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41301
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_734_length_41301_cov_21.177763"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            82..1248
                     /locus_tag="PK839_04725"
     CDS             82..1248
                     /locus_tag="PK839_04725"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOG72076.1"
                     /translation="MDCKHTRFINAFLLALPLFILSCSNGSKVTIRVEVKSPEKNMLY
                     ISRIDFGKTTPIDSAKISSGESIKKFRLPQGSEPTFYTISLKKGGALTLLASRDEKIS
                     VKFDVQNMDSYEVVGSKESLKVKEIAESFRKSKQTVDRLRKEYIKAQTDAEREAIGRQ
                     FQAEIDSQRASCSRFIWANPMSKASIMALYQKYNDDLYLFDRAEDLTLLKMVASAWRA
                     LYPESDYTRGMLEDIGRIEKIITNSKINKLISEAEPSIPDLDIPDKNGTFVKLSSLKG
                     KVILLDFFTASNTSSLLDNRELMNIYNTFKNRGFEVYQVSMDADKNEWLNYLSNTRVT
                     WISVREVDPTHSAAALLYNVTQLPTNYLIDKNFNIIDKNIYGERLKEKLNVLLR"
     gene            1314..2159
                     /locus_tag="PK839_04730"
     CDS             1314..2159
                     /locus_tag="PK839_04730"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HOG72077.1"
                     /translation="MNQNRKIYFASDLHLGHPNLRDSLPREKLFISWLDSIDADIEEL
                     YLLGDIFDFWFEYRRVVPKGYTRFMGRIAQLTDRGIPVHFFTGNHDVWMFNYFKEELG
                     VEIHHKPIEKVLYGHKVLMAHGDGLGPGDIGYKLLKRIFTSTTLQWLFARLHPNFSLW
                     FGNQWSVSSRYSKEITHVFRGDDEQITAYARQVLDSKHFDIFMFGHWHSPTIFPLTQD
                     SNLVVLGDWLVSNTYAVLDGDDFWLMKYFPDKPDVPLGHIKLSHVPEAPVLPLHDQFN
                     RINKY"
     gene            2220..2807
                     /locus_tag="PK839_04735"
     CDS             2220..2807
                     /locus_tag="PK839_04735"
                     /inference="COORDINATES: protein motif:HMM:NF016239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HOG72078.1"
                     /translation="MNFLAHLYLSGCNPEVRLGGFIGDYVKGSKFTHFTPDIQKGIKL
                     HRAIDSVTDSHPSTRACSAIFKPVYKRYSGVVSDVVFDHVLAKEWSRYSESNLNEFTQ
                     IFYTQMFQRYQLLPPTVQQFLPNMIRTNRLSSYQTSEGVREALNIMSLRTSLPNNTEY
                     AIVQLKTHYNLFFDYFHPLFNDLMQLATREFGINL"
     gene            complement(2797..4224)
                     /gene="rodA"
                     /locus_tag="PK839_04740"
     CDS             complement(2797..4224)
                     /gene="rodA"
                     /locus_tag="PK839_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HOG72079.1"
                     /translation="MQRKSNLVSNLDWGTIVLYVILVLMGWMNIYAAVYSEEHNSILD
                     FSQRYGKQLIWITFAFFLGVVVLLSNGRIYSVFAYIIYGLVILLLLSTLVFGRVVNAS
                     KSWIPLGSFGIQPAEFAKFATALALARLMSPYEFKLNTFWSYVKVGLIIAIPAGIILL
                     QNDTGTALVFGSFVLVLYREGLPWWVLGVLFLLVALFISTLVFSTPVIIVALYVTTVL
                     IYMLQRGKFKFAISLTAGLTGLGFLLYLASQTFNIQVKTDYFYIIPVGMGIVIATLYA
                     FFSRTKMIYGLVLFLCVTVGFSLSTEYVFENVLDVHHQKRINDLLGIESDPLGWGYNV
                     NQSKIAIGSGGFWGKGYLSGTQTKFNFVPEQSTDFIFCTVGEEWGFVGSLVVLALFAW
                     LLLRLIRIAERQRESFSRIYGYGVVSILFFHIAVNIGMAVGLLPVIGIPLPFFSYGGS
                     SLWAFTILVFILLRLDADRYGTFKG"
     gene            complement(4208..6061)
                     /gene="mrdA"
                     /locus_tag="PK839_04745"
     CDS             complement(4208..6061)
                     /gene="mrdA"
                     /locus_tag="PK839_04745"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOG72080.1"
                     /translation="MRFNFQADKRFTITGLIIIVFIIIAGKLFYIQVIDRSYQLSASS
                     NVLRYVTKYPARGLIFDRNGNLLVSNKVVYDLMMVKKQVVPFDTMAFASLLNITTDHV
                     RDAFVQASRQKGYSPRKSVVLLKQLPAEACAMLQEKLYLFPGFELLPRTVRLYDREVA
                     SHVLGYIGEVDEEIISKNPYYQLGDYIGISGLEKSYEEYLRGKKGVSVYMVDVYNRIK
                     GSYEDGKYDSAAVEGQNLICTLDADLQEYGEKLMQNKIGSIVAIEPRTGEVLAMVSSP
                     TYNPGLLVGRERADNFRTLQNDSLKPIFNRSIMALYPPGSTFKVVNALIGLQEGCVYP
                     QTTYSCHGGYTVGRGVGCHIHPSPLNLTQAIQMSCNTYFCHVFRNILDKPEFGSVEKG
                     FTVWKNHVESFGYGNKLNIDLPNEQKGIVPTVAFYDKYFRKGGWNSLTILSLSIGQGE
                     LAATPLQMANLVTIIANRGFYLDPHVVRAVGDSMVRNSNVPEKHYTTIDTQWFDYIVE
                     GMDMAVNGAPGSGSTARIAALPDIRICGKTGTSQNPHGADHSVFFAFAPKDDPKIAIS
                     VYVENAGFGATWAAPIASLMIEKYLRDSISRPYLEDYILQANLLDRRAKKK"
     gene            complement(6068..6577)
                     /gene="mreD"
                     /locus_tag="PK839_04750"
     CDS             complement(6068..6577)
                     /gene="mreD"
                     /locus_tag="PK839_04750"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HOG72081.1"
                     /translation="MYKDYLRYILIFILLLLIQLLVLNNINLGGYINPYVYLLFILLL
                     PYGTPRWVLLLVGFFTGLVVDYFSNTLGLHASATLILAFVRPSIMSGLRFSNNINERS
                     MLNISLTGTEWFIRYAALGIVIHHSTLFMLETFSFRFFHLTLLRILLSSLVSFVFIFF
                     LEVLLTRKK"
     gene            complement(6577..7395)
                     /gene="mreC"
                     /locus_tag="PK839_04755"
     CDS             complement(6577..7395)
                     /gene="mreC"
                     /locus_tag="PK839_04755"
                     /inference="COORDINATES: protein motif:HMM:NF010532.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HOG72082.1"
                     /translation="MNSFIRLLQRFSYIILFAVLLAVSLSMYINTSYYQKAKVGAATG
                     VITGFFSEKLHNIRSYMDIKRDNVKLSLENVELRNEVERLREVIGKCADTAGYNLADT
                     SITLIRAMVVNNSVSHRQNFITINIGINDGAYQGMGVICDGGVVGVIAATSPNYSTVI
                     SLLNTTLKVSAKHAKSGVFGTLSWNGYNYQEVELTDIPLHVGISVGDTIATSGFSSIF
                     PKDIPIGVIKDFYKKDGSFYNIRVQLLTDFRALNYVYAVRVRNITEINQIEGSR"
     gene            complement(7585..8607)
                     /locus_tag="PK839_04760"
     CDS             complement(7585..8607)
                     /locus_tag="PK839_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOG72083.1"
                     /translation="MGLFSFLTQELAIDLGTANTIILYNDKIMVDQPSIVAIDQSTGK
                     MIAIGETARAMHGKTHENIKTIRPLRDGVIADFNAAEQMIRGMISMINIKNLVFTPSL
                     RMVIGIPSGSTEVEIRAVRDSSEHAGGRDVYLIYEPMAAALGIGLDVLAPEGNMVVDI
                     GGGTTEIAVIALGGIVCNKSIRNAGDGFTSDIQAYMRHQHNVKIGERTAEDIKIKVGS
                     ALPDLENPPADLIVRGPNLMTALPIEVPVSYQEIAYCLDKSLSKIESAIFNVLELTPP
                     ELYADIVEKGIYLAGGGALLRGLDKRLTEKINIPFRVVEDPLHAVARGTSIALKNVGK
                     FPFLMR"
     gene            complement(8647..10173)
                     /gene="purH"
                     /locus_tag="PK839_04765"
     CDS             complement(8647..10173)
                     /gene="purH"
                     /locus_tag="PK839_04765"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HOG72084.1"
                     /translation="MEVIRQIRKALISVYHKENLEDIVRTLDSHNVLIYSTGGTLDFI
                     HSLGVKAIPVEELTGYPAILGGRVKTLHPKVFGAILARRENENDQFQLTEYAIPEIDL
                     VVVDLYPFEQTVVSGASEAEINEKIDIGGVSLIRAAAKNYRDVLVVSNRQQYARFSKM
                     LEENGCTTSLEQRKQFAAEAFETTSHYDVAIFNHFNNQFPQMAFKQSIQGVKELRYGE
                     NPHQKGIFYGSFDGMFEQLHGKEISFNNLMDIDAAVNLIAELDQPAIAILKHNNACGV
                     AVRDTLLQAWSDALAGDPVSAFGGVIVANRAISMDTAEAINKIFLEVLLAPDFEEDAL
                     ALLKSKKNRILLRYKDTRLPAITFRSLLNGVLVQERDSIKHDSAGLRIVTKVAPSTHQ
                     VEDMLFANVVVKHSKSNAIVLVKDKQLVGSGVGQTSRVDAVRQAIDKAKTFGFDLQGA
                     VLASDAYFPFPDNVELAAAAGIKAIIQPGGSVKDQESIDCCNRHGVAMVFTGIRHFKH
                     "
     gene            10677..11942
                     /locus_tag="PK839_04770"
     CDS             10677..11942
                     /locus_tag="PK839_04770"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG72085.1"
                     /translation="MNALKPFIILIRLARESMQFAYQSIKANKLRTFLSLFGITIGIF
                     AIISVFTMVDSLEKNIRNSLESLGNNVVYVQKWPWGGNEEYPWWKYLNRPEPTLNDMS
                     EIRRRSSLAQASCFNVSFNKQVKFQNKSLSNVTIYGTTNGYDEIRSFELGSGRFFNLF
                     ELNSGKPYAVIGATICEELFQNADPIGQSIQIGGKKATVIGVFAKEGKSAIGETSSDD
                     MVVIPLGMAKSMVDLRNSGPWIMVKARDNVVPEDLIDELRGILRSYHRLKPLDSDDFS
                     LNQIDMMKESLDAIFSSISFAGGFIAFFSIIVGGFGIANIMFVSVKERTSQIGIQKAL
                     GAKRYFILIQFLYEAMLLAILGGIIGLILIFAGTLIVTYGLDFTISLTLENIIWGLAI
                     SAIIGIASGFIPAWMASRLSPVEAINTKS"
     gene            complement(11944..12729)
                     /gene="rsmA"
                     /locus_tag="PK839_04775"
     CDS             complement(11944..12729)
                     /gene="rsmA"
                     /locus_tag="PK839_04775"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007563391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HOG72086.1"
                     /translation="MPVVRPKKSLGQHFLHDRRIAGLIVSSLQAEGVDYVVEVGPGMG
                     VLTEDLLQRFGPVFYAVEVDGESVQYLRQRLPELGGNLIEADFLDVDIENCFNGRVAI
                     IGNLPYNISSQIFFKILAMRQRVVEAVVMVQREVAQRIAEKPGSRTYGILSVLLQAYF
                     NIEYLHTVSEGAFTPPPRVKSSVIRLTRNHVAELDCNPELFVRVVKASFNQRRKMLRN
                     AIHSAFPGVEAGKPFSERRAEQLSVADFVELTNMVEQQLTAVS"
     gene            complement(12737..13900)
                     /locus_tag="PK839_04780"
     CDS             complement(12737..13900)
                     /locus_tag="PK839_04780"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOG72087.1"
                     /translation="MSDIGEQWVYVGGWNGLGSDITDSFKVSTRGRGNFSLQTTTPEM
                     LALSFDRVHFPILLCVEPGSNVEVTGTASSYQVKGSFESARINTFQQRYNSFLSEVAH
                     LSGLIPDSTATPESDSLASVLIHKRDSLISSFRTYTLSEVRANPYCLSSVPFILTTFD
                     GGQELLPYELFGNTYQKVDSCLGLAYPDKPLVQTFHQHIQMQEKKLAQLHKADRYSTG
                     DVLPAISFETVDSQAISIPGLWAKAILIEFWADWCPQSVSTADQLRILYEKHSNQGFK
                     IIRVGMGLEPEALYQRVKRDSIPWINVAIPDSEKDYWVDSIGVVYLPFNFLIDRSGRV
                     VAKNLFGDTLVRKLDDLLGANSGRLKPDVHPKTGITSKPEVKVNTITSNPETK"
     gene            14204..16147
                     /locus_tag="PK839_04785"
     CDS             14204..16147
                     /locus_tag="PK839_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HOG72088.1"
                     /translation="MKYLAFDKDQLVNLEYSLEREILSTNRAGGYLSTTIVLCNTRKY
                     HGLMILPIDEFDGENHVLLSNVDETVVQHEKVFNLGIHRYPGIYEPRGHKYIVDFEYE
                     PTPTLVYRVGGVILKKELLFVHNEEQVMIRYTLLDAHSPTKLRIKPFMAFRNVHSLSK
                     ANMWANTKYQECSNGVRYKLYTGFPFLYMQLNRQNEFVASPDWYYNVEYMEEIRRGYE
                     GHEDLFVPGYFETSIRKGESIIFSAATNEQSTPALLRKFNSELTRRPPKDSFMNCIAN
                     TARQFIIQRGKRYDIIAGYPWFSSWSRDSFISLPGLTLGLDDVKTCKALLDSLSTDLK
                     NGLFPNVRTGNSEAFNSADAPLWYFWAIQQYASYTQNYTEVWDDYGKKMKAILKGYRE
                     GLPFSIKMQPNGLIWAGEKGYAITWMDAVIQAKPVTPRIGYPVEINALWYNALMFTLE
                     LATKNKDLRFVKEWQPIADQVKKSFNELFWIPEKNYLADYVDENGQNWDIRPNQLFAV
                     SLPYSPIDDEKKIAVLNVVSHELYTPKGLRTLSPNNPNYKGRYEGDQPTRDMAYHQGT
                     AWPWLLGAYVEAQFKLQGKGALGLAQEIVDSLQEDIALHGICSVAEVYDGDPPQRPNG
                     CISQAWSVAELIRIVKMIEKYSD"
     gene            16158..17450
                     /locus_tag="PK839_04790"
     CDS             16158..17450
                     /locus_tag="PK839_04790"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOG72089.1"
                     /translation="MRVLMFGWEFPPHITGGLGTACFGLTRGLTKQKVDVIFVVPKAY
                     GDEDKSAVRLVAAEDVDVAYSAKNLEEFWRHLKYLEISSNIVPYVDPEEYAKIIEHNE
                     AETVEYESGKVKTRFKFSGKYNPNLMQEVTRYAVVAANLAAREKFDVIHSHDWLTFPA
                     GIAAKMVSGKPLVVHVHATEFDRSGENINQAVFDVEQRGMMVADKVITVSNYTRNIVI
                     TRYGIPEDKVVTVHNAVDFSDEQDMEFERGVSEQIVTFLGRVTFQKGPDYFVEAANKV
                     LKRTHNVRFVMAGSGDMLNRMIRRVAQLGIAHKFHFTGFLKGPDVNRMFHLSDVYVMP
                     SVSEPFGISPLEAMRSSVPVIISKQSGVSEVLKYAIKVDFWDIDALADSIYGLLNYNA
                     LSTFFIRHGQEEVNSLKWDNAASKVIEAYQDVLSRKNS"
     gene            17547..18722
                     /locus_tag="PK839_04795"
                     /pseudo
     CDS             17547..18722
                     /locus_tag="PK839_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
     gene            19262..23512
                     /gene="glgP"
                     /locus_tag="PK839_04800"
     CDS             19262..23512
                     /gene="glgP"
                     /locus_tag="PK839_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HOG72090.1"
                     /translation="MNDKLLKPDYLFEVSWEVCNKVGGIHTVMSTKASMIENELKNNY
                     ITVGPDLIKGIEHNAEFIEDTNLFAAWRSHAIENGMPIRVGRWNIPCKPVAILVNFSQ
                     LISKKDEIFKGFWEKFKLDSITGQWDYIEPALFGYAAGKVIESFTNFNLSLRHRIVAQ
                     FHEWMTGTGLLYLKANVPQVGTVFTTHATVLGRSIAGNNIPLYSNIEKIDPESKAREF
                     GVISKQSLERISAQQADCFTTVSDITARECSHFLKKDVDLLTPNGFDDTFIPEAEEHK
                     LRRVEGKIKFYEVAEAMLAHEVAKDSMIVGISGRYEFKNKGIDVFLDALGKLNRDNNL
                     GREVLAFLLIPAGHHGPRKDVFHNLLEKEKDSNYSILDDTHVTHYLNDPDSEPILKKI
                     RALGLNNSSSDKVKVFWVPCYLNGNDGIFNKSYYDLLIGMDFTVFPSYYEPWGYTPME
                     SLAFKVPTITTSLAGFADWVINNYKKPKDAITIIKRNDENDLEVTVQIASTIDAFTRI
                     THERYNELMNNAQEIASIALWKNFIDYYYKAYDKALSLVGSRTHQFMELERQEILPTV
                     ERKFRTTQPSWTRLIVQKNLPEKLKPLDELSRNLWWSWNTDSKKLFESIDKELWIKCE
                     KNPILFLERISLPRFQELEHDAEFVEKLHSIYLNFTAYMMQRYQRKGPKIAYFSMEFG
                     LHSSLKLYSGGLGILAGDYLKEASDRNIDMVGVGLLYRYGYFTQQLSTSGQQIAVYDQ
                     QNFTQSLALPVRDEDGRWVTVSVAFPGRQVQARIWKVDVGRTELYLLDTDFEENLVND
                     RTITHYLYGGDLENRFMQEMILGIGGIRALNELGIKADIYHLNEGHAAFTGLERLRKF
                     ILDEKLSFSEAMEVVRATSLFTTHTPVPAGHDAFPEELVRTYMSHYPDRLMISWDQFM
                     DLGRMVPGDSTQRFSMSVLATNLAQEVNGVSWLHGEVSRKMFAGMWPSYFPNELHISY
                     VTNGVHFPTWTAREWKSIMENGQATNSNGYKTPAWNELENIPDSKIWEIRNTLRKRLI
                     KLINNRLSNPEMTRFESPRQVIEIQEKLSDSILTIGFARRFATYKRANLLFKDLDRLD
                     QLVNNPEHPVQFLFAGKAHPNDKAGQDLIKSIIEIAKQPRFLGRILFLQNYDMELSRH
                     MIQGTDVWLNTPTRPMEASGTSGQKAVMNGVLHFSVLDGWWVEGYRENAGWALPMEST
                     YAESEFQDELDAEMIYTILENEIVPAFYQRNSDDIPDKWISFIRKSMTQVASNFTTLR
                     MINDYQNRFYNKLYFRSSELIENDFEVAKKISSWKRRITRNWDEIEIIQVKQFDVSRE
                     AIIMGKEYTAEVILDMGSLGPDEIGVEMVLADLIEGGEVKVKRVHEFQVQAVEGSRVT
                     YRLNLIPVEPGAFECGIRIYPKNPMLPHRMDFPLVRWA"
     gene            23649..24344
                     /locus_tag="PK839_04805"
     CDS             23649..24344
                     /locus_tag="PK839_04805"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HOG72091.1"
                     /translation="MDNLVEQLEVKFLQKGNPAVALQQKKYMRNQFEFFGIQSADRRE
                     IQKLLFTPEHLPAKNELGSTINRLWLKPQREFQYFAQEMAYKFHKQLELADISLYENM
                     VTTKSWWDTVDYIASRLMGEYFASFPEQRKPFIQKWVTSDNLWLKRSALIFQLNYKQQ
                     TDEVLLAATIHSLLPSSEFFICKAIGWVLRNYSKVNPEWVLQFVAHTPLPPLSKKEAT
                     RLIVQPPLRHKQV"
     gene            24497..24856
                     /gene="rpsF"
                     /locus_tag="PK839_04810"
     CDS             24497..24856
                     /gene="rpsF"
                     /locus_tag="PK839_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010265188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HOG72092.1"
                     /translation="MNHYETVFIATPVLSEAQMKETVTKFKDFITQDGGEIIHEESWG
                     LRKLAYPIQKKTTGFYHLLEFKAPGELIDKLETQYRRDERIIRFLTVKLDKYAVEYAE
                     KKRSNKVEKVEPKTMED"
     gene            24860..25129
                     /gene="rpsR"
                     /locus_tag="PK839_04815"
     CDS             24860..25129
                     /gene="rpsR"
                     /locus_tag="PK839_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005637107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HOG72093.1"
                     /translation="MSQNQSEIRYLTPPTVEVKKKKYCRFKKNRIKYIDYKDPEFLKK
                     FLNEQGKILPRRLTGTSQKYQRKVSTAIKRARHLALLPFVTDLMK"
     gene            25151..25597
                     /gene="rplI"
                     /locus_tag="PK839_04820"
     CDS             25151..25597
                     /gene="rplI"
                     /locus_tag="PK839_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009276783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HOG72094.1"
                     /translation="MEIILLQDMPSLGSKNDIINVRNGYARNYLIPKGFATIATESAK
                     KVLAENTKQRAHKEAKIKADAEELAKKLNGLKLTIGAKTSSTGKIFGSVNTIQISEAL
                     AEKGFEIDRKLIFIQDDQIKEVGSYTAEVKLHKEVKVSIEFDVVSE"
     gene            complement(25645..26022)
                     /locus_tag="PK839_04825"
     CDS             complement(25645..26022)
                     /locus_tag="PK839_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HOG72095.1"
                     /translation="MKKIINTNDAPRAIGPYSQAVEANGFLFVSGQVPVNPSTGKIVE
                     GGIAEQTEQVVKNIGAILAKAGLTYANVVKTTCLLSDMENFATMNEVYARYFTAEMPA
                     RAAFGVVKLPLGALVEIECIAIR"
     gene            complement(26104..28608)
                     /locus_tag="PK839_04830"
     CDS             complement(26104..28608)
                     /locus_tag="PK839_04830"
                     /inference="COORDINATES: protein motif:HMM:NF040057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HOG72096.1"
                     /translation="MPLQDTVPATDTIKPAAGKSVLEDPVISNAEDSITYSIDGQTAY
                     LYGNAVVTYQNLELKAKYIEFNMATKQVYACGLPDSTGKVVGRPVFKEGGQTYEMDEI
                     HYNFDTKRAKITGVVTEQSGGFMHSKVTKMLEDQVVNLQDGKYTTCDLDHPHFYIAIT
                     KGKMIPDKKIIAGPAYLVIEDVPLPIGIPFGFFPNTRKRQAGVILPEYGEENIRGIFL
                     RGGGVYFGMGDYMDEKLTGDYYSKGSWGLRSQTTYKVRYRFSGTISLEASSYVTSERG
                     LPDYSKDNTYWIRWNHTQDAKANPNSTFQANVNFGSSNHNRYNARNINNALANTFNSS
                     ISYSRIWPGSPFSMSASLNHSQNSIDKSVDLGFPKVSLNMSRIYPFKRKLSVGKPSWY
                     EKIGLSLNTNLENRVQVKEDNLFKSSVVDSMQNGVRHDIPISTSFNLLKFITISPSAS
                     YNEFWYLQTINKHWDPDSQVVVVDTIKGFKRGYQYSTAVSMSTKIYGTFQFGKKSKVQ
                     AIRHVITPGISLSYRPDFAKESFGFYKSVQVDSTGRMAQYSIFERGIYGGPGGGKSGV
                     VNFSLGNMVEMKVLSPKDTANPVKKVKILEGFSINTSYNMLADSLNWSDISVSGRTNL
                     FNNININFSGAFSPYAIDAKGSRYNRFELDRSGKLARFTRGSVGLDFALNSSKGSASS
                     GGDQSRSQGMGGSNPPLGTAPDGSSFGESLGTQYGMDYVDFSVPWNLRVSYNLSYSKP
                     AYVSSLVQTLTFSGDINLTPKWKIAFSSGFDLKSRQLTTTSLNFYRDLHCWEMRLTVI
                     PIGYYKSFSFQINVRSSILQDLKLTKRDHYLDNLGE"
     gene            28766..29944
                     /locus_tag="PK839_04835"
     CDS             28766..29944
                     /locus_tag="PK839_04835"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOG72097.1"
                     /translation="MEGLRLKHFLPIPLLVVLSLLLAVGEKALSQTSSSYQLTRVVVD
                     AGHGGKDPGTVGRVSKEKDIALSIALKVGSYIEKNFPEVKVIYTRNDDTFIPLDERSR
                     IANEAKADLFISIHCNSAPNRTSFGTETYVMGLHRSADNLDVAMRENAVISYEDDYST
                     RYEGYDPSSSESYIIFSMLQNAFLDQSLSLANLIQTDFRDRASRKDRGVKQAGFLVLW
                     KTSMPSVLVEVGYLSNASEEKYLNSQEGQDYIASSIYRAFKEYKKNVDSYSNQLAQIQ
                     LTKPADSSVEQKGNTQVQPPSSTSQSHDFPIFKVQLMASNKPIPLDSPNFQNIKTVEE
                     LIINDSYKYCVGSLSSYDEALKLCREVRKQFPDAFVIATKNGVPVALQQAIKDNKTKN
                     "
     gene            30146..31141
                     /locus_tag="PK839_04840"
     CDS             30146..31141
                     /locus_tag="PK839_04840"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HOG72098.1"
                     /translation="MKIKLTKEAKIGIFMVACLFAMFWGINFLKGKNVFSSNNTYYVV
                     FTSVDGLKPTNDVLINGFKVGLIEEIEFNDIKSGKFKVNLLISKKYKLPRNTIAKLIS
                     ADLMGGKAIKLEISSDTAYYQPGDTLPGVIEVGLIDQLGHQMTPVKEKAEQLMVQLNN
                     TLLVVEKVFNEENRDQLSQGIANLNASLNNISQISAALDTMLNSPKGSLKTTFSNIES
                     VSSNLKKSNKELTNIIQNVSSVSDSLADINLAQTFTKLETTLNDIQAIIAKVNSGEGT
                     LGNLVSNDSLYTNLQKVTLSLDLLINDIKNNPKRYVSFSLIDLNRSQSSGEKVKE"
     gene            31477..32922
                     /gene="dnaA"
                     /locus_tag="PK839_04845"
     CDS             31477..32922
                     /gene="dnaA"
                     /locus_tag="PK839_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007210310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HOG72099.1"
                     /translation="MNVLDFKEVWQNCLNIIRDNVPPISFKTWFEPIVPVRLEQNVLT
                     IQVPSPFFYEYLEEQYIDILRKTLRKELGSDAKLEYSVVMEHGGYNNGTPYTVKFPTQ
                     NKTEIKNKPVSIQNLQEPPIKNPFIIPGIKKLHVDPQLNQEYTFANFLEGDCNRLARS
                     AGMAISQKPGNNPFNPFFIYGNSGLGKTHLAQAIGIEVKQKFPEKTVLYVNAAKFQTQ
                     FVDAHIKNNNINDFIHFYQSIDVLILDDVHEFAGKEKTQDAFFHIFNHLHQTGKQLVL
                     TSDQPPAELQGLEQRLLSRFKWGLSADLQTPDFATRVAILKRKVYNDGIVIPEEVIEY
                     IASNITQNIRELEGALISMLAQSTLNGREITIELASEMIEKLVKNPARKDLSIDYIQK
                     VVCEYFGLPVDVLQAKTRKREIVQARQIAMFFSKNLTKSSLSTIGAVIGSKDHATVLH
                     ACKTINNLMETDKRFKAQIEDIEKRIKYSNQ"
     gene            33079..35415
                     /locus_tag="PK839_04850"
     CDS             33079..35415
                     /locus_tag="PK839_04850"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG72100.1"
                     /translation="MKALKRVALPVIVGFLAVLGTAAAPLATDSLARILNQAGQDTAS
                     LNAVFKKLISVDLPNPTQTDSLLNELLSRFKKLNYNDGVNRVLSFRGDLYYQQGNFQQ
                     ALEVEKELASKYGGSKTNMLPLANAYKMIAGCFFNMGIHDSAVVYAEKSISLFKNLND
                     SQNVARSLNLLGGIYWNMGNLTKASEKLYESLRLREQLNDSLGMANAYNNIGLIYDSQ
                     GKLTEAMKMYESALEIYNKLNNSKGIGRVCNNMAIILKNQKKFSESLEMFLKSLEVDK
                     KLNNIDDQGKTLNNIGQLYIEMGNIQAAIGYYQQARQLFLRSGNENGLAATLLNMGKA
                     SYDQKNYAESRTYYLQSLEVAKRINSTEWIRDSYHGLYLLAKQAGRASEALTLFERYK
                     SLDDSLRGLENMNKIDQLRVEFETEKKEREITLLTKDNELKTEKLNRQKTYNLLLLTI
                     TIAVVAFLGLTALAIKRLRADKKILVDKNQEIQAQKEEIESQRDLLEVTNTELNQQKE
                     ELQAQTDQIERQNYIIASANQRMTEGLEYASLIQKSLSSDINSFGKHFSGYSLLYMPK
                     DIVSGDFFWVWDENRDDNFTFAIADCTGHGVAGAFMSLMAINLLRDAVGVNNITEPQK
                     ISTFLYNELIRKKASIFDSNLIIGLDFIVCSYSPKSQVLCYSGNHLNFYLVREGEVNK
                     LKVERNIDRQSATLIFAQSSIKLKKNDRLYFTTDGFADQISETKRKKIGNSDFQNLLL
                     SVGNYPIKQHSELLAEYFSKWKGTYEQVDDVLVLGLEV"
     gene            complement(35412..36251)
                     /gene="cysQ"
                     /locus_tag="PK839_04855"
     CDS             complement(35412..36251)
                     /gene="cysQ"
                     /locus_tag="PK839_04855"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005836050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HOG72101.1"
                     /translation="MIEQPEIINLLVTAINTSVRAGSKIMEVYNSDDFQVNLKSDRTP
                     LTLADRVAHEEIKNTLYKTLIPVLSEEGRSIHYVERKGWEYFWIVDPLDGTKEFIKRN
                     GEFTVNIALMYEGFPILGVIYVPVTECLYYSIHPEGSFKVTGIKPTMEVNLTYSDVCS
                     GAKRLPLSQNHSGIVVVESRSHNSPETLEFISNLDKKYGGITRISCGSSLKMCMVAEG
                     IADIYPRLSLSSEWDTAAGQAIVEGAGFQVISFDSGDRMSYNKEELINPWFIVRNPAR
                     FEV"
     gene            36493..37947
                     /locus_tag="PK839_04860"
     CDS             36493..37947
                     /locus_tag="PK839_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005789241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HOG72102.1"
                     /translation="MLSSLEPNNLWKFFEEICKIPRPSKKEKLVIAYLQNFAREHGLK
                     VRTDHAGNVLVEKPAYPGFEKLKPVVLQCHMDMVCEKNSDVAHNFQTDPIVPGIDGEW
                     VKANGTTLGADDGIGIAAQLAILESTEIKHGPLECLFTVDEETGLTGANELEKNFFSG
                     KILINLDSEDEGELFIGCAGGVDTLATFAYDTEKVPTNSTAFRLTVKGLKGGHSGDDI
                     DKGLGNSIKIINRFLWRGVNSYDLRLSMLDGGNLRNAIPREAYAIFTIHEEDRAALRK
                     EFTLIKNDILAELHDVEPDLSITFENADLPPYVVDEPTQYDLLNALYACPHGVISMSR
                     QLKGLVETSTNLASVKFIQDNQILVTTSQRSSVESAKKDISNMVESVFRLANARIQHS
                     DGYPGWTPNTNSEILGITRNSYIRLFGVEPVVRAIHAGLECGLFLEKYPELDMISFGP
                     TIKAAHSPYERLNIPSTMKFWKLLLDVLQNIPSI"
     gene            complement(37987..38145)
                     /locus_tag="PK839_04865"
     CDS             complement(37987..38145)
                     /locus_tag="PK839_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004372607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HOG72103.1"
                     /translation="MYKCRICGYIYNPDEGDPASGIPAGTPFEELPESWHCPICYATI
                     EYFDELPE"
     gene            complement(38282..38662)
                     /locus_tag="PK839_04870"
     CDS             complement(38282..38662)
                     /locus_tag="PK839_04870"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HOG72104.1"
                     /translation="MAELSSLYIKGTSKTPQIDFKPGMLQVSGRSIPEDAVEFFQPVI
                     GWLEEYLKSPEPLTRLNLRMEYINSGSNRFIFNLIKMLDDAYLKGHSVLVNWYYEEDD
                     DTIRNLGRDFQALVKVPFKMVEIV"
     gene            complement(38919..40121)
                     /locus_tag="PK839_04875"
     CDS             complement(38919..40121)
                     /locus_tag="PK839_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HOG72105.1"
                     /translation="MKRSVFAACFIVTALFSQLLFGQEPQKESESYVFTPIKELKVTP
                     VKNQNRSSTCWSFSGVAFLESELLRMGKPEVNLSPMFVVRNAYVEKADRFVRFNGTNN
                     FGPGGSFFDVVEMVKKYGIVPMEVYPGLNYGEDNHVHGELDAVTRSFVDEIVKNPNKK
                     LSTGWKPAFIGIVDGYLGQVPASFTYNGKTYTPITFAQSLGINLDDYVCIGSYTHHPF
                     YSKFVLEIPDNWIHGEVYNLPINEFERVLDNAIDNGYTIAWGSDVSEKGFSWRNGVAV
                     VPDINSAENAGSDKDHWVQLSQKEKEAKVYSFEKPLKEMVITQELRQQAFDNYQTTDD
                     HGMLIMGKAKDQNGNIFYKVKNSWGSEGKYNGFFYASKPFVLLKTTDIMVHKNAIPDD
                     IARKLNLK"
     gene            complement(40229..40978)
                     /locus_tag="PK839_04880"
     CDS             complement(40229..40978)
                     /locus_tag="PK839_04880"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HOG72106.1"
                     /translation="MKRIDFTVEWHNSVTSSNSICAQRCEEGAAEGLVIAAMFQEMGR
                     GQRGNSWESEAGRNLTFSILLRPGHLRVDEQFYLSKVAAASVCDWVSAYVSGDDVTIK
                     WPNDIYIGDSKVAGILIENSFSSAMLGTSVVGIGINLNQQTFPDDIPNPTSLRLATGM
                     EFDLHSSLAEFLECFARRYELLSDEQSRFQLDEYYLKKLYRRDLYHGYCDKNGNFKAK
                     IAGVKPTGELVLQLPTGELRVYAFKEVMFLI"
BASE COUNT        12257 a         9723 c         8908 g        10413 t
ORIGIN      
        1 tttaccctcg atttccccct ccacgctatt tgaaagttga acataatggt tacttttgca
       61 ttcggtaaat agaaaacaaa aatggattgc aagcatacga gattcataaa tgcctttctt
      121 ctggccctac cgctgtttat tctttcatgc tccaatggca gcaaggttac cataagggtt
      181 gaggtgaagt caccagaaaa aaacatgctt tacataagcc ggattgactt tggtaaaaca
      241 accccaatag actcagctaa aatatcatcc ggagaaagca ttaaaaaatt taggttaccc
      301 cagggcagcg aacccacatt ctacaccatc agcctgaaaa aagggggagc acttactcta
      361 ctcgcaagca gggatgaaaa gatttcggtt aagttcgatg tccaaaacat ggacagctac
      421 gaggtggtag gctcaaagga atcgttgaaa gttaaggaaa ttgccgaatc atttagaaaa
      481 tcaaagcaaa ccgttgacag gcttagaaag gagtacatca aagcccaaac tgacgccgaa
      541 cgtgaggcta ttggaaggca gtttcaggca gaaatagact cgcaaagagc cagctgcagc
      601 aggttcatct gggctaaccc tatgtcgaaa gccagcatta tggctcttta ccagaaatat
      661 aacgatgatc tctacttgtt tgaccgggct gaggatttaa ccctgctcaa aatggtagcc
      721 tcagcatggc gggcactcta ccccgaatcg gactatacca ggggtatgct tgaggatatt
      781 ggcaggattg agaaaattat taccaactct aaaataaaca agctgatttc ggaagcagaa
      841 ccatctattc ccgacctgga catccctgat aaaaatggta cctttgtaaa gctatcatca
      901 cttaaaggaa aagtaattct tcttgacttt ttcaccgcat ccaacacctc aagcctactc
      961 gacaaccgtg aattgatgaa tatttataac acctttaaaa ataggggttt tgaggtttac
     1021 caggtttcca tggatgccga taaaaacgaa tggcttaact acctgtcaaa tacccgagta
     1081 acatggattt cggtcaggga agttgatcct acacattcag ctgctgccct actttacaac
     1141 gttacacagc tgccaacaaa ctacctgatt gacaagaact tcaacattat tgataaaaac
     1201 atatacggtg aaagacttaa ggagaagctg aatgtccttc taaggtaggc taagcctaaa
     1261 cgccaagtaa ccattcagca tgtagagatg ttcaaaaaca cttatttaag ccggtgaatc
     1321 agaatcgaaa aatatacttt gcctccgacc ttcaccttgg gcatccaaat cttagagata
     1381 gcctcccccg tgagaagctt ttcatatcgt ggctcgatag tattgatgct gatattgagg
     1441 agctctacct gctgggtgac atttttgact tctggtttga gtaccgcagg gtggttccaa
     1501 agggatatac ccgtttcatg ggacgtatag ctcagctcac cgataggggg ataccggtac
     1561 atttcttcac cggaaaccac gatgtttgga tgttcaacta ttttaaagag gagctaggtg
     1621 tcgagattca ccataaaccc atagagaagg tgctttacgg gcataaggtg ctgatggcgc
     1681 atggtgacgg ccttggaccg ggagatattg gctacaagct gttgaaacgc atttttacca
     1741 gcaccaccct tcagtggctt tttgccaggc tacacccaaa cttttcactt tggtttggga
     1801 accagtggag cgttagcagc cgctactcaa aagagataac ccatgttttc agaggcgacg
     1861 acgagcaaat aaccgcttac gcccggcagg ttttggatag caagcatttt gacattttca
     1921 tgtttggtca ctggcattca ccaactattt ttccccttac gcaggattcc aacctggttg
     1981 tgctaggcga ctggctggtt agcaacactt atgccgtgct cgatggtgac gatttttggc
     2041 ttatgaagta tttccccgat aaacccgatg tgcccttagg gcatatcaag ctcagccatg
     2101 ttcccgaagc accagttttg ccgcttcatg atcaatttaa cagaattaat aaatattagg
     2161 tagatcggct tattcagcgc ttccgccagt gcaattaacc aaaagcaaac atcaaccctg
     2221 tgaattttct agcccatctc tacctttcgg gttgtaatcc tgaagtcaga ttgggcggct
     2281 tcattggcga ctatgtaaaa ggtagtaaat ttacccactt cactcccgat attcaaaagg
     2341 gtattaaact tcatagggca atagacagtg ttaccgatag ccacccttcc acccgagcct
     2401 gttcggcaat ttttaaacca gtgtataagc gctactcggg tgtggtgagt gatgtagttt
     2461 ttgaccatgt tttggcaaaa gagtggagcc gctactctga aagtaacctg aatgaattca
     2521 cacaaatctt ctacacccaa atgtttcagc gctaccagct tctcccccct accgttcagc
     2581 aattcctccc caacatgata cgcaccaacc ggctttcgag ctaccagaca agtgaagggg
     2641 ttcgggaagc actcaacatt atgagcctcc gaacctcact tcccaacaac acggagtacg
     2701 ccattgtgca gctgaaaact cactacaacc tatttttcga ttattttcac ccgcttttca
     2761 acgatttaat gcagcttgcg accagggagt tcggcatcaa cctttaaatg ttccatacct
     2821 gtcggcatca agccttagca ggataaaaac caggatagta aaagcccaca acgatgagcc
     2881 tccgtagcta aaaaaaggta aagggattcc tataacggga agtagcccaa ctgccatccc
     2941 aatgtttact gcaatatgaa aaaataggat ggacactacc ccgtacccgt atatcctaga
     3001 aaaactctca cgttgtcgtt cggcaatcct gataagacgc aagagcagcc atgcgaaaag
     3061 cgcaagaact accaaagaac ctacaaaacc ccactcctca cccaccgtgc agaaaataaa
     3121 atcggtactt tgctcaggaa caaagttgaa ctttgtttgg gttcccgaaa ggtatccctt
     3181 tccccaaaac ccacccgaac caatggcaat tttggattgg ttaacattgt agccccatcc
     3241 cagcggatcg gattcaatgc caagcaaatc attaattcgc ttctgatggt gaacgtcgag
     3301 aacattctca aaaacatact cggtggaaag cgaaaaacct actgtaacgc ataggaaaag
     3361 cactaacccg taaatcattt tcgtcctcga aaagaaagca tataacgttg ctatgacaat
     3421 acccatgcct acagggataa tataaaaata atcggttttt acttgtatgt tgaatgtttg
     3481 tgaggcaagg taaagtagaa agcccaaacc ggtaaggcct gctgttaggc ttattgcaaa
     3541 tttaaacttc ccccgttgca gcatgtatat taaaacggta gtaacataca gcgcaactat
     3601 aataaccggc gtgctgaata ccagggttga aataaaaagc gcaaccagca aaaaaagcac
     3661 tcccaacacc caccatggca atccctcgcg gtagagcaca agcacaaaag aaccgaaaac
     3721 caatgccgtt ccggtatcat tctgtagcag aataataccg gcaggaatag caatgattaa
     3781 accaacttta acgtatgacc aaaatgtatt tagcttgaac tcgtatgggc tcattagcct
     3841 ggcgagagca agtgcagtgg caaacttggc aaactccgca ggctgtattc caaatgatcc
     3901 gagtggaatc catgattttg aggcgttaac caccctgcca aacaccaggg tggaaaggag
     3961 caaaagaata accagcccgt agatgatgta ggcaaaaacg gaatatatcc ttccgttgct
     4021 taacaaaaca acgactccca ggaaaaaggc aaaagtaatc caaatcaact gcttgccata
     4081 gcgctggcta aaatcgagta tgctgttgtg ctcctcgctg taaactgcgg catagatgtt
     4141 catccacccc atcaacacaa ggatgacgta aagcacgatg gttccccaat cgaggttgct
     4201 taccaggtta ctttttcttt gcacggcggt cgagtaggtt tgcttgaagt atatagtctt
     4261 cgagataggg acgggatata gagtcccgta ggtacttttc aatcattagg ctagcaattg
     4321 gcgcagccca agttgcacca aacccggcat tttccacgta caccgaaatg gcaattttag
     4381 gatcatcttt gggagcaaag gcaaaaaata cggagtggtc ggctccatgt ggattttgtg
     4441 atgttcccgt tttcccgcaa atgcgaatat cggggagagc agcaattcgg gcggtacttc
     4501 cgctcccggg ggctccgtta acggccatat ccattccctc aactatatag tcgaaccact
     4561 gggtgtctat tgtggtgtag tgcttttcgg gaacatttga gtttctcacc atgctgtcac
     4621 ctacagctct caccacatgg ggatcaaggt aaaaccccct gttggcaata atggttacca
     4681 ggtttgccat ttgcaagggt gttgctgcca gctctccctg cccaatggaa agcgagagga
     4741 tggtaaggga attccagccg ccttttctaa agtacttgtc gtagaacgca actgtcggaa
     4801 caatcccttt ctgctcgttg ggtaaatcaa tattcagctt attaccgtaa ccaaatgatt
     4861 cgacatgatt tttccagacc gtaaaaccct tttcgactga tccaaattcg ggcttatcga
     4921 gtatgttccg gaacacatgg cagaaatagg tgttacacga catctggatg gcctgagtta
     4981 gattcaatgg agaggggtga atgtggcatc ccacacctcg cccaacggtg tatcctccat
     5041 ggcagctata ggtggtttgc gggtaaacgc aaccttcctg tagcccaata agcgcattca
     5101 caaccttaaa ggtagaaccc ggtgggtaaa gggccatgat ggagcggtta aaaattggtt
     5161 tgagcgaatc gttttggagg gtcctgaagt tatcggcacg ctcgcggcca acaagtaatc
     5221 ctggattata cgtagggctt gaaaccatgg ccagcacctc gcctgttctg ggctcaatag
     5281 ccacaatgct gcctatctta ttctgcatca acttttcgcc atactcctga aggtcggcat
     5341 ccaaagtgca aatcagattc tgcccctcta cggcagcaga atcgtatttt ccatcctcat
     5401 acgagccctt gatgcggttg taaacgtcaa ccatgtaaac gctaacaccc tttttcccac
     5461 ggaggtactc ctcatacgat ttttccaaac cgctgatacc aatataatcg cccagctggt
     5521 agtagggatt ttttgaaata atttcctcgt caacctcgcc aatgtatccc agaacatgac
     5581 ttgctacttc cctgtcgtaa agcctaacag tacgtggcag cagctcaaat cccggaaaca
     5641 ggtaaagctt ttcctgaagc attgcacatg cctcggccgg aagctgctta agcagcacaa
     5701 ccgatttgcg cggggagtac cctttttggc gggaagcctg tacaaacgca tcccttacat
     5761 ggtcggtagt aatgttcaac agggaggcaa atgccatcgt atcaaagggt acaacctgct
     5821 tcttcaccat catcaggtcg taaaccacct tgttcgaaac cagcaggttt ccgttccggt
     5881 cgaatatcaa cccacgagcc ggatacttgg ttacatacct taacacgtta ctgcttgccg
     5941 aaagctggta cgacctgtct atcacctgaa tatagaatag cttccccgct atgatgataa
     6001 acactatgat gattagcccg gtaatggtga atcgtttatc agcctgaaaa ttgaacctca
     6061 tagcccatta cttttttcgg gtgagtaaaa cttcaaggaa gaaaataaac acaaacgaaa
     6121 ccaggctgct aagtaggatt ctcagtaggg tgaggtgaaa aaagcggaag ctgaaggttt
     6181 caagcatgaa tagggtagaa tgatgaataa ctattcccag tgctgcatag cgaataaacc
     6241 actcggtgcc tgtaaggctt atgttgagca tgctccgctc gttgatgtta ttgctaaacc
     6301 ttagaccaga cattatcgac gggcggacaa aagcaagtat tagcgtagcc gaagcgtgaa
     6361 gtccaagggt atttgaaaag taatcgacaa ccaatcccgt aaaaaagcct acaagcagca
     6421 agacccagcg gggagtgcca tagggtagca aaagtatgaa taacaggtaa acgtaagggt
     6481 tgatataccc tcccaggtta atattattaa gtaccaggag ctgaatcaga agcaacagga
     6541 taaagatcag aatgtatcgc aagtaatcct tgtacatcac ctactcccct caatttggtt
     6601 aatctccgta atgttcctaa ccctaactgc gtacacgtag ttaagcgccc gaaaatcggt
     6661 aagcagctga acacgaatgt tgtagaagct accatccttc ttgtaaaaat ccttgataac
     6721 accaatgggt atatcctttg gaaaaatgga tgagaaaccg ctagtggcaa tggtatcgcc
     6781 aacagaaatg ccaacatgca ggggtatatc ggttagctca acctcctggt aattataacc
     6841 attccatgac agggtaccaa aaacacccga cttggcatgc ttggcagaaa ccttaagggt
     6901 tgtattaagc agcgatataa ccgtgctgta gttgggcgat gttgccgcta taacccctac
     6961 gactcctcca tcgcaaatca cacccatgcc ctgataagcg ccatcgttta taccaatgtt
     7021 aattgttata aagttctgac ggtgggatac ggagttgttc acaaccattg ccctaatcag
     7081 ggtaatgctg gtgtcggcaa ggttgtagcc tgccgtgtcg gcacatttcc cgatgacttc
     7141 tctaagtctt tcaacctcgt ttcgaagctc aacattctca agcgatagct taacattatc
     7201 ccttttaata tccatgtagc tacgaatatt gtggagcttc tcagagaaga aacctgtaat
     7261 aaccccggtt gccgcaccaa cttttgcctt ctgatagtag gaagtattga tgtacattga
     7321 aagtgaaacg gccagtagca ccgcaaaaag aatgatatac gaaaatcgtt gaagtagccg
     7381 aataaagctg ttcatcctcg atactcttaa ttaccagcgt caaaacatca ggttgaaaat
     7441 attaaaaaac gctccaacaa ccatttacaa gggtgctatc aggtgcttca tttttttgtg
     7501 tagccatcca gacgagctgg atatagaaat atttttccta acggagccac acggggctta
     7561 atgattttta aactctgtta ccctttaacg catcaggaat gggaatttgc caacattttt
     7621 cagtgcaatg ctggtacccc tagctacagc atgaagcggg tcctccacaa ccctaaaggg
     7681 tatgttaatt ttttcggtaa gccttttatc gaggcctcta agcagcgcac ctccacctgc
     7741 caggtaaatt cccttctcaa caatatcggc gtaaagttca ggaggtgtta gctcaagcac
     7801 gttgaatatt gccgattcaa tttttgaaag cgatttgtcc agacagtagg caatttcctg
     7861 atacgaaacc ggaacctcaa taggcagggc agtcataagg ttggggcctc ggacaatcag
     7921 gtcagcagga ggattctcca agtcgggaag tgcagaaccg acctttattt taatatcctc
     7981 ggcagtgcgt tcgccaatct taacattatg ctggtgcctc atgtaggctt gtatgtcgga
     8041 tgtaaaaccg tcaccggcat tccgaatgga tttgttgcaa acaataccac ccagggcaat
     8101 aacggcaatc tcggttgttc ccccacctat gtccaccacc atgttccctt cgggagcaag
     8161 cacatcaagg ccaataccca gggcggcagc catgggttca tagataaggt aaacatccct
     8221 accgcctgca tgctccgagg aatccctaac agcacgaatt tctacttcgg tgctgccgct
     8281 tggaatacct attaccattc tgagagaggg ggtaaaaacc aggtttttta tgtttatcat
     8341 gcttatcata ccccgaatca tctgttcggc agcgttaaaa tcggcaatca cgccatcacg
     8401 aagcggtcgg atggttttaa tgttctcatg ggttttacca tgcattgccc tggcagtctc
     8461 tccaatggct atcatcttgc cggtgctttg gtcaatagcc acaatggagg gttgatccac
     8521 cataatttta tcgttgtaaa ggatgatggt gttagcggtt cccaggtcta tggcaagctc
     8581 ctgggttaaa aaagaaaaaa gtcccatatt ctatgtctag ttatattatt gcttagtgtt
     8641 tattgcttag tgtttaaaat gtcttatgcc tgtaaaaacc atagcaactc catgcctatt
     8701 gcagcaatct atgctctcct gatcttttac ggatccacct ggctgaataa tagccttaat
     8761 accagcagca gcagctagct caacgttgtc gggaaaaggg aaataggcat ccgacgccag
     8821 aactgctccc tgaaggtcaa agccaaaggt ctttgcctta tcaatagctt gcctaacggc
     8881 gtcaaccctt gaagtttgac caacgccgct tccaaccagc tgtttatctt taaccagcac
     8941 aatggcgttg gacttcgagt gcttcaccac aacgttggca aaaagcatgt cctcaacctg
     9001 atgggtgctt ggagcaactt ttgttactat ccgaaggcca gcgctatcat gctttatgga
     9061 atcacgctcc tgaaccaaca cgccattcag caacgagcga aatgttattg ctggaagccg
     9121 ggtgtcctta tacctcaaga gtatcctgtt tttctttgac tttagtaggg caagtgcatc
     9181 ttcctcaaaa tcgggggcaa gcagaacctc cagaaatatt ttattaattg cttctgccgt
     9241 atccatgctg attgcacggt tggccacaat cactccgcca aaagccgaaa ccggatcgcc
     9301 ggccagcgca tcgctccatg cctgcaaaag ggtatcacgc acggctactc cacatgcgtt
     9361 gttgtgcttc aaaatagcga tggcaggttg atcgagctct gcaatcaggt ttactgcagc
     9421 atcaatatcc attaggttgt tgaaggatat ttccttgcca tggagttgtt caaacatccc
     9481 gtcgaaacta ccataaaaaa ttcccttttg atggggattt tcaccgtagc gtaactcttt
     9541 caccccttga atgctctgct tgaaagccat ttgcggaaac tggttattga aatggttgaa
     9601 aatggcaaca tcatagtgtg atgtagtttc aaatgcttca gcagcaaact gcttacgctg
     9661 ctccaggcta gtagtgcatc cattttcctc aagcatttta ctaaacctgg catactgttg
     9721 acggtttgaa acaaccagaa catcccggta attcttagca gctgcccgaa tcaacgaaac
     9781 acccccaata tcaatttttt cattaatttc agcctccgag gcaccagaga ctaccgtttg
     9841 ttcaaacggg tacaggtcaa ctactaccag gtcaatttcg gggattgcat actcggttag
     9901 ctggaactgg tcattctcat tttcgcgacg tgccagtatt gcaccaaaaa cctttggatg
     9961 aagggtttta acgcgccctc cgagtatggc tggatatcct gtaagttctt ctactggaat
    10021 ggcttttacc cccaggctgt ggataaaatc taaggttcca cctgttgagt aaattaaaac
    10081 gttgtggcta tcaagtgtcc ttacaatatc ttccaggttt tccttatggt aaacagaaat
    10141 taaggccttc cttatttgtc ttatgacttc cattgcctga ttttcctact gatacaaatt
    10201 aaaagcccca aatatttgga gtggtaaaaa tggttaaatc ctttgagaat tcaatgctga
    10261 acagcaaaaa atatataaac aaaaatttcg gggatgattg gggtaagagt aacaggttaa
    10321 gagtaataaa aaaagtaagt tgtaaaactt tataaaacac ccatgttgtg cagccagcgt
    10381 gagcacgcat caacaatcgg ttgttagttg cgagttcggc gaagccaaaa tatgggagat
    10441 ccatcccctc aaccccaaac ttaaaaggta tgggccaagg tttttgtaat agaaaaccta
    10501 aacattttgt acggataaaa ttattaaaag caagtgtacg ccagaattca tagtggttaa
    10561 acaaatatac tccttcaaaa attctgtttc caccagcgca ttcagagtaa ttaacaaatc
    10621 catttggcta gaccataatt ttttaaatac ctttgctttg tttaacatgt atcagaatga
    10681 atgccctaaa acccttcatc atactcatca ggcttgcccg ggaaagcatg cagtttgcct
    10741 accagtccat aaaggccaac aagctacgga cattcctctc gctgtttggc ataaccattg
    10801 gaatttttgc catcatctcg gtatttacta tggttgacag tctggaaaaa aacatccgga
    10861 acagcttgga atcgttagga aataacgtgg tttacgtgca aaagtggccg tggggtggca
    10921 atgaggaata cccctggtgg aagtatctaa accgaccaga gcccacacta aatgacatga
    10981 gcgaaattcg tcgccggagt agcctggctc aagccagctg tttcaacgtg tcgtttaaca
    11041 agcaagtcaa gtttcagaac aaaagcctgt caaacgtaac tatttacggg actaccaacg
    11101 gatacgacga gataagatcg tttgagcttg gcagcggcag gtttttcaac cttttcgagc
    11161 taaactccgg caagccatac gctgtgatag gtgctacaat ttgtgaggag ctattccaaa
    11221 atgctgatcc tattggtcaa agcatacaaa ttggtgggaa aaaagccacg gttataggcg
    11281 tttttgccaa ggaaggcaaa tcggccattg gcgaaaccag ctcagacgat atggtggtaa
    11341 tccctctagg catggccaag tctatggttg acctgcgaaa ttctggccca tggataatgg
    11401 tcaaagctcg cgataacgtt gtccccgaag acttgattga cgagttgagg ggaatactcc
    11461 ggtcatacca tcgcctaaaa ccgcttgaca gcgacgactt ctctctaaac cagatagaca
    11521 tgatgaagga gagcctcgat gcaattttca gctccattag ctttgctggt gggtttatag
    11581 ccttcttctc aataattgtt ggtggatttg gaattgccaa catcatgttt gtttcggtta
    11641 aggagcgaac cagccagatt ggcattcaaa aggcgcttgg ggcaaagcgc tacttcatac
    11701 tcatccaatt cctctacgag gccatgcttc tggcaattct gggtggcatt attggactaa
    11761 tccttatatt tgctggaacg ctgatagtta cctacgggct tgattttacc atatcgctta
    11821 cactcgagaa catcatctgg gggcttgcca tttcggctat tataggaatt gcatctggtt
    11881 ttatcccggc gtggatggcc tccaggttaa gtccggttga ggcaatcaac acaaaatcgt
    11941 aggctaactc accgctgtga gctgctgctc caccatgttg gtcaactcaa caaaatcggc
    12001 taccgaaagc tgttcagccc tcctctccga aaagggctta ccagcttcaa ccccgggaaa
    12061 cgctgagtga atcgcatttc gcagcatctt tcgacgttgg ttaaacgaag ctttaactac
    12121 ccgtacaaag agttccgggt tgcagtctag ctctgccaca tggttacggg ttagcctgat
    12181 gaccgacgat ttcaccctgg gtggtggggt aaacgcgccc tcgctcactg tatgcaaata
    12241 ctcaatgttg aagtatgcct gaagcagcac gctaagaatg ccgtaggttc tggaaccggg
    12301 cttttcggca atgcgttgcg ccacctcgcg ttgcaccatc accactgcct ccaccaccct
    12361 ttggcgcatg gcaagaattt taaaaaatat ttgggacgaa atgttgtagg gcaggttacc
    12421 aataatggca actcgtccat tgaagcaatt ttcaatatca acatccagaa aatcggcttc
    12481 tataagattg ccgcccaact ccggtaagcg ctgcctaagg tactgcaccg actcgccatc
    12541 aacctccacc gcataaaaaa cagggccgaa tcgctggagc aagtcctcag taagcacgcc
    12601 catacccggc cctacctcaa caacgtagtc aacaccttcc gcctgaaggc tcgaaacaat
    12661 caaccctgca atcctacgat cgtgtaggaa atgctgcccc aaactctttt taggccgaac
    12721 aacaggcatt aacttgctat ttagtttcgg gattgctagt aattgtgttt acctttacct
    12781 caggcttaga agtgatcccg gtttttggat gaacatcggg ctttagccga ccagaattcg
    12841 cgccgagcaa atcatccagc ttgcgcacaa gggtatcgcc aaataaattt ttagccacca
    12901 ccctaccgga tcgatcgata aggaagttga atggaaggta aacgacacca atcgaatcaa
    12961 cccagtagtc tttctccgag tcgggtatgg ccacatttat ccacgggatg gagtccctct
    13021 taaccctctg gtagagagcc tcaggctcta accccatacc aacacgaatg attttgaacc
    13081 cttggttgga gtgcttttcg tagagtatgc gcagctggtc ggctgtgctc accgactgtg
    13141 ggcaccagtc agcccaaaac tcaataagaa tagctttagc ccaaagtccg ggaatagata
    13201 tcgcctggct atcaacagtt tcaaaactta tagctggcaa aacatctcca gtggaatacc
    13261 tatcagcctt atgcaattgt gcaagttttt tttcctgcat ttgaatatgc tggtgaaaag
    13321 tttgcaccag aggtttatcg ggatatgcta agcccaagca ggaatcaact ttctggtaag
    13381 tattgccgaa gagttcgtaa ggtagaagct cctgccctcc gtcaaaagta gttaaaatga
    13441 agggaactga ggacaggcag taggggttag ccctcacctc tgaaagggtg tatgtcctaa
    13501 agctggaaat aagtgagtct cttttatgaa taagtaccga tgctaacgag tcgctctcgg
    13561 gagtggctgt gctgtctgga attaagccgc tgagatgagc tacctcagat aaaaaactat
    13621 tatagcgttg ctgaaaagtg tttatcctgg cgctttcaaa tgaacccttc acctggtatg
    13681 aacttgcagt accggttacc tccacatttg aacctggttc aacacataac aggattggaa
    13741 aatgaaccct gtcgaacgaa agtgcaagca tttcgggtgt tgtggtttgt agagaaaagt
    13801 taccccttcc gcgtgtgctt accttaaaag aatcggttat atcgctgcct aatccgttcc
    13861 atcccccaac ataaacccat tgctctccta tgtcactcaa tttaaggcta atgcttgctc
    13921 gtttatagga attagtgcag gagctaattg caattgcagc tggcaggaca aaaaggaacc
    13981 taaaaaaagt tgttgaatgg taatattttg aagggtgcat aaaactttta taaaaattaa
    14041 cactgggtgc aaacgaaatt ttcgataaat tcaaaaattt gctcctcaaa agtagcaaaa
    14101 agtttcccca aaggggtgat tcaatatgat tttttgataa cttgtagcag gaataaaacc
    14161 taaaaatttt cataacctaa atatattaaa attaaccttt gccatgaaat atctagcatt
    14221 cgacaaggat caacttgtaa atctagagta ctcactagag agggaaatac ttagcaccaa
    14281 cagggctggt ggatatttaa gtaccacgat tgtactctgc aacacccgta agtaccatgg
    14341 gctaatgatc ctgcctattg atgagtttga tggtgaaaat cacgtgctgc tttcaaacgt
    14401 tgatgaaacg gtggtgcagc acgaaaaggt attcaacctg ggcattcacc gctatccagg
    14461 aatttacgaa ccacgtggac ataaatacat tgttgatttt gagtacgagc caaccccaac
    14521 gctcgtttac cgggtgggcg gtgtaatact aaaaaaagag ctgctttttg ttcacaatga
    14581 ggagcaggtg atgatacgtt acaccctgct ggatgctcat agcccaacaa agttaaggat
    14641 aaagccattc atggctttca gaaatgttca tagcctcagc aaggcgaata tgtgggctaa
    14701 cactaaatac caagaatgca gcaatggcgt aaggtataag ctctacaccg gctttccgtt
    14761 cctctacatg cagctgaaca ggcaaaatga gtttgttgca tcccccgact ggtactacaa
    14821 tgtggagtat atggaggaaa tcagaagagg atatgaggga catgaagacc tatttgtgcc
    14881 cggatacttt gaaacctcca tcagaaaggg agaaagtata attttttcgg ctgccaccaa
    14941 cgaacagtca acccctgccc tactgcgtaa attcaactcc gagctaaccc ggcgaccacc
    15001 caaggacagc ttcatgaact gcattgccaa cacagcgcgc cagtttatca tccaacgggg
    15061 aaaacgctac gatattattg ccggctatcc gtggtttagc agctggagcc gcgactcgtt
    15121 catttcacta cccggattaa cccttggact cgatgatgtt aaaacctgca aagcactgct
    15181 cgactcgcta agcaccgatc taaaaaacgg gttattcccc aacgtcagaa caggcaattc
    15241 cgaagcgttt aactccgccg atgcaccact ttggtatttc tgggccattc aacaatacgc
    15301 cagttataca caaaactaca ccgaagtgtg ggacgattat ggcaaaaaaa tgaaggctat
    15361 cctgaaaggt tatcgcgaag ggctaccctt tagcattaaa atgcagccaa atggcttgat
    15421 ttgggccggt gaaaagggct acgctatcac atggatggat gccgttattc aggcaaaacc
    15481 ggttaccccc cgcattggct acccggttga aataaatgct ctatggtaca acgccctgat
    15541 gtttaccctt gaactggcta ccaaaaataa agatttaagg tttgtgaaag aatggcaacc
    15601 cattgcagat caggtaaaaa aatcattcaa cgagctattc tggattccag agaaaaacta
    15661 cctggccgac tacgttgacg aaaatggaca aaactgggac atcaggccca accagctttt
    15721 tgcagtttct ttaccctact cgccaattga tgatgagaaa aaaattgccg tgctcaacgt
    15781 tgtaagccat gagctttaca ccccaaaagg attaagaacc ctttcgccga acaacccaaa
    15841 ctacaaggga aggtatgagg gcgatcaacc aacacgcgat atggcctacc atcagggaac
    15901 agcgtggcct tggcttttag gagcctacgt tgaagcacaa tttaaacttc agggtaaagg
    15961 cgctttagga ttggctcagg aaatagttga ttcgctgcag gaagatattg cactacacgg
    16021 aatttgctcc gtggcagagg tttacgacgg cgatccacca caacggccca atggctgcat
    16081 atcgcaggca tggagcgttg ccgagctaat cagaattgta aaaatgattg agaagtatag
    16141 cgattaacca aacgcgtatg agagttttaa tgttcggatg ggaattccct ccccatataa
    16201 ccggaggttt aggaacagcc tgctttggct taacgcgtgg acttaccaag caaaaagttg
    16261 acgtcatctt tgttgttcca aaagcttacg gcgatgagga taaatcggct gtgaggttgg
    16321 ttgctgccga agatgttgat gtggcctact ccgcaaagaa cctggaggag ttctggaggc
    16381 accttaagta tctagagatc agctccaaca ttgtccccta tgttgacccc gaagagtacg
    16441 ctaaaattat cgagcataac gaggcagaaa ctgttgagta tgaatccggc aaggtaaaaa
    16501 cgcggtttaa gttttctgga aaatacaacc ccaacctgat gcaggaggtt acccggtatg
    16561 ctgttgtagc agccaacctg gctgcaaggg aaaagttcga cgttatccac tcgcacgact
    16621 ggctaacctt tccggcaggt attgccgcca agatggtttc gggcaaaccc ctggtagtgc
    16681 atgttcacgc caccgaattt gacaggagtg gggagaacat caaccaggca gtattcgatg
    16741 tggagcaaag gggaatgatg gttgccgaca aggttatcac cgtgagtaac tatacccgca
    16801 atatagtaat tacccgctat ggaataccag aagataaggt ggttacagtg cataacgctg
    16861 tggacttctc cgatgagcag gacatggaat tcgagcgcgg ggtctccgag caaatagtta
    16921 ccttcctggg cagggtaact tttcagaagg gtcccgacta cttcgttgaa gctgccaaca
    16981 aggtgctcaa gcgaactcat aacgtacgct ttgttatggc tggaagcggt gatatgctaa
    17041 accggatgat tcgtcgggtg gcccagcttg gcattgccca caaatttcac tttaccggat
    17101 ttcttaaagg cccggatgtg aacaggatgt tccacctctc cgacgtttac gtcatgccct
    17161 ccgtttcaga gcctttcgga ataagcccat tagaagcaat gcgttccagc gtacctgtta
    17221 tcatttccaa gcagtcgggt gtatctgagg tgcttaaata cgccataaaa gttgatttct
    17281 gggatattga tgcactcgcc gatagcattt atggcctgct gaactataat gcattatcta
    17341 cgttttttat caggcacgga caggaagagg tgaatagctt gaagtgggat aatgccgcca
    17401 gcaaggtaat tgaagcatat caagatgtgc taagcaggaa aaattcttaa aaacatatac
    17461 tccagtaggt atgaatcgtc cagctggtca taaacaacag tgtagatacc gcaacttatt
    17521 actataaagt taaaaatttt gagctgatga aaacaatttg cttctacttt caggtgcatc
    17581 aaccatatag gctccgaagg taccgcttct tcgatattgg gaaaaaccat agctacttcg
    17641 acgattttgc caaccgctcc atcatgcgga aggtagccga aaagtgctac ctaccgacca
    17701 accagcttat gctcgaccta atcaacgagt ttggcacaag gtttaaaatt tcctactcca
    17761 tttcggggac tgcgctcgac cagtttgagc aatacgcacc ggacgtactg gacagctttc
    17821 gcaggttggc caaaaccgga agtgtggagt ttttggccga gacctactcc cactccctct
    17881 cctcgctcaa gagcaaggaa gaattctacc ggcaggttga agcccaacag aataaaattg
    17941 agaaactcct tggatacaag cccaaaacct tcagaaacac cgagcttata tattccgatg
    18001 aaattggcgc catggttgcc gatttggggt ttaaaaccat gctcaccgaa ggcgccaagc
    18061 atattctggg atggaaaagt tccaacttcg tgtatgccaa cgctataaat cctaagttga
    18121 agctgctgct gaaaaatttc cagctaagcg atgacatcgc cttccgcttt tccaaccgcg
    18181 actggaacga gtggccgcta accaccgaaa agtttgtttg gtggctcaac aacattgatc
    18241 ccaaggagga aattgttaac ctttttatgg attatgaaac ctttggtgaa caccagtggg
    18301 ctgaaaccgg aatattcgat ttccttcgtg ccctgccatc tagggttttc ggtcactcca
    18361 attttgaatt ccttacccca tcggaggctg cagagaaaca ccaaccggtt gccctgcttc
    18421 acgttcccta ccctatttcc tgggccgacg aggaaagaga ccttaccgca tggttgggca
    18481 acgaactgca ggatagcgct ttcaaccaac tatatgcgct taccgataag gttgaaaaaa
    18541 ttgatgaccc cgagatcaaa agggactggc tttacctcca aaccagcgac cacttctact
    18601 acatgtgcac caaatggttt agcgatggcg atgtgcacaa gtacttcaac ccctacggtt
    18661 caccctacga ggcattcatc aactacatga acatcctaag cgactttacc cttagggtta
    18721 aggataagct actcgaaaaa acattggtcg atgaatcatc agctgaacta gacaaccccc
    18781 aaaagctcat taaactctat gaggataaaa ttaacgagct aaagaatcag ctaaatgagc
    18841 aacaagcaaa gcctaccggc aaaactggca aagctaaagt tgataatcct gacgctgcca
    18901 gcacaccagg taataaggca aaaacaagca gcaagtctac aaaggctgtt gctaaaccaa
    18961 agagtaaaac tacgcctaaa agcaagcctg ttgtaagcac caaaactgaa gaaatagcgc
    19021 taaagccatc ttcaaaaaag aagaacagta tcaaaagtag cccaacaact gccaaatcca
    19081 gtaccaaatc aagtaattca aagtcaacca ctaagggaac aaaaaagaaa aaagagtagg
    19141 ttaatcggta gaaactgcca aataatgaca aaccggtagc aacaaatgaa atggttaacc
    19201 tatttatttt ggattaaact tggaaaaaat caaaatacta ccataaaaat attgtgaacg
    19261 aatgaatgac aagctgctaa aacccgatta cctttttgaa gtaagctggg aggtgtgcaa
    19321 caaggttggt gggatacaca ccgttatgtc aacaaaggca tcgatgattg aaaatgagct
    19381 gaagaacaac tacattactg ttggccccga cctaataaag gggattgagc ataatgctga
    19441 attcatagaa gacaccaacc tatttgccgc ctggcgtagt catgccattg aaaacggaat
    19501 gcccataagg gttggccgat ggaatatacc ctgtaaaccg gtagctattc tggtcaactt
    19561 ttcccaactt atatcaaaaa aagatgagat tttcaaaggc ttttgggaaa aattcaagct
    19621 cgattccata accggacaat gggactatat tgaacccgct ctatttggct atgcagcagg
    19681 caaggtgatt gaaagtttta ccaacttcaa cctaagctta cgacatcgca tagtagccca
    19741 gtttcacgag tggatgaccg gaacaggatt gctctaccta aaagcaaatg ttccacaagt
    19801 aggaacggta ttcacaaccc atgctacggt gctaggacga agtattgctg gtaacaacat
    19861 accgctgtat agcaacatcg aaaaaattga ccctgaaagc aaggccagag aatttggtgt
    19921 tatctccaag caatcgttag aaagaatcag tgcccagcaa gccgactgct tcaccactgt
    19981 tagcgatata accgccaggg aatgcagcca ctttcttaaa aaggatgttg acctacttac
    20041 ccctaacggt ttcgacgata cctttatacc cgaagctgaa gaacacaagc tgcggagagt
    20101 tgagggtaaa attaaatttt acgaggtggc agaagccatg ctggcgcacg aagttgcgaa
    20161 ggatagcatg attgttggta taagcggacg ctacgaattt aaaaacaagg ggatagatgt
    20221 ttttttggat gccctgggca agctgaatag ggacaataac cttggcaggg aggtgctggc
    20281 attcctgctt atacctgccg gacatcatgg cccccgaaag gatgtttttc acaacctact
    20341 cgaaaaagaa aaagatagca actatagcat ccttgacgat actcacgtta cccactacct
    20401 aaacgacccc gatagcgaac caattcttaa aaaaataagg gcgcttggct taaataactc
    20461 atcatccgac aaggtaaagg tgttttgggt tccctgctac ctgaacggta acgacggaat
    20521 attcaacaaa agctactacg acctgctaat cggcatggac ttcaccgttt tcccttccta
    20581 ctacgagcct tggggctaca cccccatgga aagcttagct ttcaaagttc caacgataac
    20641 cacctcactt gcaggatttg ccgactgggt gataaacaac tacaaaaagc ctaaagacgc
    20701 aattaccatc atcaaacgta acgacgaaaa cgatctggag gtaactgtcc aaattgcctc
    20761 caccattgat gccttcaccc ggatcaccca tgagcgctat aacgagctga tgaacaatgc
    20821 gcaggaaatt gcatctattg ccctttggaa aaactttatt gactactact ataaagctta
    20881 cgataaagcc ctttcgctgg ttggtagccg aacccatcag ttcatggagc tcgaaaggca
    20941 ggaaattctg cctacagttg agcgcaaatt ccgaaccacc caaccctctt ggacccgcct
    21001 gattgttcag aagaaccttc ctgaaaagct aaaacccctt gacgagcttt cacgaaacct
    21061 ttggtggtcg tggaataccg attccaaaaa actatttgaa tccatcgaca aggaactctg
    21121 gataaaatgt gagaaaaacc ccatactatt cctcgagaga attagcctac cgcgttttca
    21181 ggagctggag cacgatgctg aatttgtgga aaagcttcac tccatatacc tgaattttac
    21241 cgcctacatg atgcagcgct accagcgcaa agggcctaaa atagcctact tcagcatgga
    21301 gtttggcctc cacagctcac tgaagctcta ctcgggtggg ttgggcatct tggccggcga
    21361 ctacctcaaa gaagcctccg acaggaatat tgacatggta ggtgttggcc tactttaccg
    21421 ttacggttac tttacccagc agctatccac ctctggccag caaatagccg tatacgacca
    21481 gcaaaacttc acccaatcgc tggccttacc agttcgcgac gaggatggca gatgggttac
    21541 agttagcgtg gcattccctg gaaggcaggt gcaggcacga atctggaagg ttgatgtggg
    21601 tagaacagag ctttacctgt tggacaccga cttcgaggag aacctcgtaa acgaccggac
    21661 aattactcac tacctttacg gtggcgacct ggaaaacagg tttatgcagg aaatgattct
    21721 gggaattggc ggaatcagag cgctaaatga actgggaata aaggccgaca tttaccacct
    21781 taacgagggt catgcagcat ttacaggact ggaaaggctt agaaagttta ttctcgatga
    21841 aaaactttca ttctccgaag ccatggaggt tgtacgggct accagcctgt ttaccaccca
    21901 tacacccgtt ccggccggtc atgatgcctt ccccgaagaa cttgtgcgaa catacatgtc
    21961 ccactacccc gacaggctga tgatttcatg ggatcagttc atggacctgg gtcgtatggt
    22021 acccggtgat tccacacaaa gattttcgat gagcgttctg gcaacaaacc tggcccagga
    22081 ggtaaatggg gttagctggc ttcacggtga ggtgagccga aaaatgttcg ccggaatgtg
    22141 gccaagctac ttccccaacg agctacatat cagctacgtt accaatggcg tccatttccc
    22201 aacatggacc gcacgggagt ggaaatcaat tatggaaaac ggccaagcca ctaacagcaa
    22261 tggttacaaa actcctgcct ggaacgagtt ggagaatatt ccagattcaa aaatttggga
    22321 aattcgtaat accctcagga aaaggctcat caaacttatc aacaacaggt tgagtaaccc
    22381 cgaaatgaca cgcttcgaaa gtccacggca ggtaattgag atacaggaaa aacttagcga
    22441 tagcatcctc accattgggt ttgcccgccg ttttgcaacc tacaagcgcg ccaacctcct
    22501 ttttaaagat ttggaccggt tggaccagct ggtgaataac cccgaacatc ctgttcaatt
    22561 cctctttgcc ggaaaagcac accctaacga taaggcagga caagacctca taaaaagcat
    22621 cattgagatt gccaagcaac cgcgcttcct tggacgaatt ctgttcctgc aaaactacga
    22681 catggagctc tccaggcaca tgattcaagg taccgatgta tggctcaaca cccctacccg
    22741 accaatggag gcttcgggta ccagcggcca aaaagcagta atgaacggcg tgcttcactt
    22801 cagtgtcctg gatggctggt gggttgaagg ttaccgcgaa aatgccggat gggcacttcc
    22861 catggagagc acctacgccg aatccgagtt tcaggatgaa cttgatgccg aaatgatata
    22921 caccattctg gaaaacgaaa ttgtacccgc attctaccaa cgtaacagcg acgatatacc
    22981 ggataaatgg atttcattca tccgtaaatc catgacccag gtagcatcta acttcaccac
    23041 ccttaggatg ataaacgact accagaacag gttttacaat aagctctact tccgttcctc
    23101 cgagcttata gaaaacgact ttgaggtagc caaaaaaatt tcgtcatgga aacgacggat
    23161 tacccgtaac tgggatgaaa ttgaaatcat tcaggtcaaa cagtttgatg tttcgcgcga
    23221 ggccattatc atgggtaaag agtacaccgc cgaggttatc ctggacatgg gttcccttgg
    23281 tcccgacgaa atcggggttg agatggtgct ggccgaccta attgaaggag gcgaagtaaa
    23341 ggttaaacgg gtccatgaat ttcaagttca agcagttgaa ggctccaggg ttacctacag
    23401 gctcaacctc atacctgtag agccgggtgc ttttgaatgc ggaatacgca tatatcctaa
    23461 aaaccccatg cttccccatc gcatggattt cccgttggta cgctgggcat agcaaaaacc
    23521 atgcagttca gcaccccata cttgtttatc cacgttgtgc ggataagcct tgctttgatt
    23581 gggccgaacc ttgataataa tcctgtagca gtagcttaaa tataatacga aaaactacaa
    23641 ccaagcaaat ggacaacctc gtagagcagc tggaagtaaa attcctacaa aagggcaatc
    23701 cagcggtggc gcttcaacaa aaaaaataca tgcgaaatca gtttgagttt ttcggcattc
    23761 agtcggctga ccgacgagag attcaaaagc tattgtttac accggaacac ctgcctgcta
    23821 aaaacgaact cggctcaacc atcaacaggc tctggcttaa accccaacgg gaatttcaat
    23881 actttgccca agaaatggcc tacaaattcc ataaacaact ggagctagcc gatataagcc
    23941 tatacgagaa catggtaacc accaaatcgt ggtgggatac ggttgactac attgcctcac
    24001 ggctcatggg cgagtacttt gcctcatttc ctgaacaacg gaaaccgttc attcaaaaat
    24061 gggttacctc ggacaacctt tggcttaaac gctcggcact aatcttccag cttaactaca
    24121 agcagcaaac cgacgaagta cttctcgccg ccacaattca ttccttgctg cctagcagtg
    24181 aattttttat atgtaaggcc ataggatggg tgcttagaaa ttatagcaaa gtcaaccctg
    24241 aatgggttct ccaatttgta gcgcacacac ccttgcctcc cctcagcaaa aaagaagcta
    24301 caaggttaat tgtccaacct ccactgcgcc ataaacaggt ttaggcttca acaagttttc
    24361 cctgaacatg agggtgcaaa ccgcaaacac cttaagccga tgctatttat tcttgagaat
    24421 tctaccctct gtgtatttgg aagatttaaa aattagctat aattttgcac cctattaact
    24481 aaaatcatat tttatggtga accattacga aaccgttttc attgctactc ccgttttgtc
    24541 tgaggcccag atgaaggaaa cggtcactaa attcaaggat ttcatcaccc aagatggggg
    24601 tgaaattatc catgaagaga gctggggatt aagaaagttg gcctacccca ttcagaaaaa
    24661 aacaaccgga ttttaccacc tgttagagtt taaagcaccc ggtgagctga ttgataagct
    24721 ggagacacag taccgtcgcg acgagcgtat tatccgtttc cttaccgtta agctcgataa
    24781 gtatgctgtt gagtatgccg agaaaaagcg cagcaacaag gtagaaaagg tggaacctaa
    24841 aacaatggag gattaagcca tgagccagaa tcaatcagaa ataagatacc tcacaccccc
    24901 aactgtagaa gtcaagaaaa agaagtactg ccgtttcaag aaaaacagga ttaaatatat
    24961 tgactacaag gatccggaat ttctaaaaaa atttttaaac gagcagggga aaattcttcc
    25021 ccggcggtta accggaacct cgcaaaagta ccaacgaaag gtatctactg ccattaagcg
    25081 ggctcggcat ttagccctac tgccatttgt aaccgatttg atgaaataat taaaggtagg
    25141 agataagcaa atggaaatca tactattaca ggatatgccc agtttgggaa gtaaaaacga
    25201 tattataaac gttcgaaacg gttacgctcg aaactatctt attcctaaag gctttgccac
    25261 cattgccaca gaatcggcca aaaaagtgct ggccgagaac accaagcagc gagcccataa
    25321 ggaggcaaaa attaaagccg atgccgaaga gctggctaaa aagcttaacg gcctgaagct
    25381 taccattgga gctaaaacca gctcgaccgg taaaattttt ggttcggtga acaccattca
    25441 gatttcggag gcgcttgccg agaaaggatt tgaaattgac cgcaagctaa tcttcattca
    25501 ggacgaccaa attaaagagg ttggctccta caccgccgag gtaaagctgc ataaggaggt
    25561 gaaggtcagc atcgaatttg atgtagtatc ggaatagcac tactcatcca gcataaaaaa
    25621 acctgccgtt ggcaggtttt ttttctacct tatggctatg cactcaatct ccaccaaggc
    25681 tccaagcggg agtttaacaa ccccaaaggc agcccttgcc ggcatttcag ctgtaaagta
    25741 acgagcatat acctcgttca tggtggcaaa attctccata tcgctaagca ggcaggtagt
    25801 tttcaccacg ttggcatagg taaggccggc cttcgccaga attgccccta tgtttttaac
    25861 tacctgctcg gtttgctcag caattcctcc ctctacaatt ttaccggttg atggattcac
    25921 gggaacctgc cccgacacaa aaagaaaccc attggcctca acagcctggc tgtatgggcc
    25981 aattgcccga ggagcatcat ttgtgttgat aatttttttc atgattaaca aattttagtg
    26041 taataaatat agatttcggc aaaaatttat catgctagta agcaatcaag aaaatcggcc
    26101 cggttattcg cccaggttat cgaggtagtg gtcgcgcttg gtaagcttta aatcctgaag
    26161 tatggaggag cgaacgttta tctgaaagct gaagcttttg tagtatccaa taggtataac
    26221 ggtcagccgc atctcccagc agtgcaggtc tctgtagaaa ttgagggacg tggttgtaag
    26281 ctgccggctc ttcaaatcga acccggagct aaaggcaatt ttccattttg gagtcaggtt
    26341 gatatcgccc gaaaaggtca gggtctgaac taaggaggat acataggcag gtttggagta
    26401 gctcaggttg tagcttaccc tgaggttcca tggaacgcta aaatcgacat aatccatgcc
    26461 gtactgtgtg cccagagact caccaaacga cgaaccatcg ggtgcggttc ctaagggggg
    26521 attgcttcca cccattccct gcgacctgga ctgatcccca cctgaagagg cgctaccctt
    26581 gctgctattc aacgcaaagt ctagcccaac gctgcccctg gtaaaacgcg ccagcttgcc
    26641 gctcctatca agttcaaacc tgttgtacct gcttccctta gcatcaattg catagggact
    26701 aaatgcaccg gaaaagttaa tgttgatgtt gttgaaaaga ttggtgcggc cgctaacgga
    26761 gatatccgac cagttcagag agtcggccag catgttatag gaggtattga tgctgaaccc
    26821 ttccagaatt tttacctttt taaccgggtt agcagtatcc tttggcgaga gcaccttcat
    26881 ttcgaccatg ttacctaaag aaaagttcac gacccccgat tttccacctc ccggcccacc
    26941 gtaaattccc ctttcaaaaa tggagtactg tgccatcctt ccggttgaat ccacctgaac
    27001 agacttgtag aatccaaaac tctccttggc aaagtcggga cggtagctca gcgaaatacc
    27061 gggtgtaatc acatgcctga ttgcctgcac ctttgatttc tttccgaact ggaatgtccc
    27121 ataaatcttt gtactcatgg acacggcggt gctgtactgg tatccccgct tgaagccttt
    27181 tatggtatca accacaacta cctgtgaatc gggatcccag tgcttgttga ttgtttgcaa
    27241 gtaccagaac tcgttgtagc tagcacttgg gctaatggtt ataaacttta gcaggttaaa
    27301 tgaggttgaa ataggtatat cgtgcctcac gccgttttgc atgctgtcaa ccaccgagct
    27361 tttaaacagg ttatcctcct tcacctgcac cctgttttca aggtttgtgt taaggctcaa
    27421 cccaatcttt tcgtaccacg agggtttccc aacgctcaat ttacgcttga atgggtaaat
    27481 acggctcatg ttgagcgaaa ctttcggaaa acctagatca acagacttgt ctatgctatt
    27541 ttgcgaatgg ttaagcgaag ccgacatgct gaagggtgaa cccggccata ttcttgagta
    27601 cgaaatgctg gagttgaaag tgtttgccag ggcattattt atgtttcggg cattgtaccg
    27661 gttatggttg gatgagccaa agttgacgtt ggcctgaaag gtggagttgg ggtttgcctt
    27721 agcatcctgt gtatggttcc accttatcca gtaggtgtta tccttactat agtccggcaa
    27781 gccacgttca ctggttacat atgatgatgc ttctaggctt atagtgccgg aaaaccggta
    27841 gcgaaccttg taggtggttt ggctgcgtaa cccccatgag ccctttgagt agtaatcgcc
    27901 cgtaagcttc tcgtccatgt agtcgcccat gccaaagtaa acacccccac ccctcaggaa
    27961 aattcccctg atattttcct cgccatactc tgggagaatt acccctgctt gccgttttcg
    28021 ggtgttggga aagaacccaa agggaatgcc aattggtagc ggtacatcct cgataaccag
    28081 gtatgcaggc ccggcaataa tttttttatc gggaatcatt tttcccttgg ttatggctat
    28141 gtaaaaatgt ggatgatcca aatcacaggt ggtgtactta ccatcctgca ggtttactac
    28201 ctggtcttca agcatcttgg ttaccttgct gtgcatgaaa ccaccagact gctcggtaac
    28261 cacgccagta atcttagccc gcttagtatc gaagttatag tgtatttcat ccatctcgta
    28321 ggtttggcca ccctctttaa acaccggacg accaaccact ttccccgtgc tatcgggcaa
    28381 accgcaggcg taaacctgct tggttgccat gttaaactcg atgtactttg cctttagctc
    28441 cagattttgg taggttacca cagcgttgcc atagaggtag gccgtttgcc cgtcaataga
    28501 gtaagtaata gaatcctcag cattacttat cacagggtct tccagcacgc ttttgccagc
    28561 tgcaggttta attgtatcgg tagcgggcac cgtatcctga agtggcacct gcaatacaaa
    28621 attgggcgaa aaaggtatgg cctgcctagc aaaccctaat gagctatata aaacaataaa
    28681 aagcgttgtt accctgaatt tcaattttag tcgtattttt gtaacaaaat tgagctcaaa
    28741 actactaaaa aatttttagg gaataatgga ggggctgcgc ttaaaacatt ttttaccaat
    28801 acccttgctg gtggttctat ccctcttgct ggcggttgga gaaaaagctt tgagccaaac
    28861 cagcagctcc tatcagctca ccagggtggt agttgatgcg gggcatggag gtaaggaccc
    28921 aggaacagtt gggcgggtaa gcaaggaaaa ggacattgcc cttagcattg ctctaaaggt
    28981 aggaagctac attgaaaaga acttccctga ggtgaaggta atctacacca gaaacgacga
    29041 cacctttata ccgttagacg agaggagtag aattgccaat gaagcaaaag ccgacctctt
    29101 tatctccatt cattgtaatt ccgccccaaa cagaacttct tttggcaccg aaacatacgt
    29161 gatggggttg caccgatcgg ccgacaacct ggacgttgcc atgcgtgaaa acgctgtaat
    29221 ttcgtatgaa gatgactact cgacaaggta tgagggttac gacccaagtt cttccgagtc
    29281 gtacataatc ttctccatgc ttcagaatgc ctttcttgat caaagcctct cgcttgccaa
    29341 cctgattcaa actgacttta gagacagggc tagccgaaag gacagagggg taaagcaggc
    29401 cgggttcctt gtgctatgga aaacctctat gccaagcgtc ctggttgaag tgggttacct
    29461 cagcaacgcc agcgaagaga agtacctgaa tagccaggag ggtcaggact acattgcctc
    29521 ctcaatttac cgagccttta aggaatacaa gaaaaacgtg gatagctaca gcaaccagct
    29581 ggcacaaata cagctcacta agccagccga cagcagcgtt gaacagaaag gcaacaccca
    29641 ggttcaaccc ccatccagca caagccaaag ccatgatttc ccaattttta aggtgcaact
    29701 aatggcctca aacaagccaa tccccctcga ttcacctaat tttcagaata ttaaaacagt
    29761 tgaagagctt atcataaacg attcctataa gtactgcgta ggcagcctga gctcctacga
    29821 cgaagccctt aaactctgta gggaagtgcg taagcaattt cccgatgcat ttgtaattgc
    29881 cacaaaaaat ggagttcctg tagcgctaca gcaagccatt aaggataaca aaaccaaaaa
    29941 ttgataggtg gaaattttac ttacccttat tcaggtataa actgactaca cgtacagaac
    30001 cagctaacac taatcaccat tacaaacaat agcaataaat attttaaata gccgcgggca
    30061 acgtgaagag aaaattaccc cagattgatg tggttgtgta tctttgccat ctgccagcta
    30121 gttaaatgtt aatactactt tagaaatgaa aataaaactt accaaagaag caaaaatagg
    30181 gatattcatg gtggcctgct tgtttgccat gttttggggt atcaactttt taaaggggaa
    30241 aaacgtgttc agctccaaca atacatacta tgtagttttt acatcggttg atgggctaaa
    30301 acccaccaat gatgttctca tcaacgggtt taaggttggc ctgatagaag aaattgagtt
    30361 caacgacatc aaatcgggga agtttaaggt taaccttttg attagcaaga agtacaagtt
    30421 gcctcgaaac accattgcca agctaatcag cgccgattta atgggcggta aggctattaa
    30481 gctggaaatt tcgtccgaca cggcctacta ccaacccggc gataccctac ctggagtcat
    30541 tgaggtaggc ctgattgacc agctgggcca ccagatgacc ccagttaaag aaaaagctga
    30601 gcagctcatg gttcagctga acaatacgct gctggtagtt gaaaaagttt tcaacgagga
    30661 gaatcgcgac cagctatcac agggtatagc taacctgaat gccagcctca acaacattag
    30721 ccagataagc gcggcactcg acaccatgct gaactcccct aagggctcgc tcaaaaccac
    30781 ctttagcaac atcgaatcgg ttagcagtaa cctcaagaaa agcaacaagg agcttaccaa
    30841 cattattcaa aacgtgtcgt cggtgagcga ctcattggcc gacatcaacc tggcacaaac
    30901 cttcacaaag ctggaaacca ccctgaatga catccaggct ataatagcta aagtaaactc
    30961 cggagagggc acccttggca accttgtcag caacgacagc ctttacacca acctgcaaaa
    31021 ggtaactttg agcctcgacc tgcttattaa cgacatcaag aacaacccca aaaggtatgt
    31081 tagcttctca ttaatcgatc taaacaggtc gcaatcctcc ggcgagaagg ttaaagaata
    31141 gcctgaaacg taaacatctt aatatctgca tgttaacccc aacatgcaca gggttgaata
    31201 gttacagtgc agctgggtaa acaacaagct taatattgta ttaacaaata gttaacattc
    31261 aaatttttac gttatcaaca tgatatctaa ttgttaatta gctaattaaa aaatttttac
    31321 gaatgaaaaa aaattattac catgatagct aacctgttgt aatgttaaag ggaaattcaa
    31381 gccctaacca cctttttttt taaaaaaatt cattacaatt ttgttgagga aatagaaaag
    31441 agtaaaaaaa aatcataagg ttttttagct atggtaatga atgttttaga ttttaaagag
    31501 gtatggcaga actgcctgaa tattataaga gacaacgttc ctccgataag cttcaaaaca
    31561 tggtttgagc caatagttcc agtaaggctg gagcagaatg tgctcaccat acaggttccc
    31621 agcccattct tttatgagta tttggaagag cagtacatag acattctccg gaaaacactg
    31681 agaaaagagt tgggatcgga tgcaaaattg gagtacagcg tagtgatgga gcatggcggg
    31741 tacaacaacg gcacccccta tacggtaaag ttccccactc agaataaaac cgaaattaag
    31801 aataaaccgg tatccataca gaacctacaa gaaccgccaa taaaaaatcc ttttataatc
    31861 cccggtataa aaaaactcca tgttgacccc cagctcaacc aggaatacac ctttgcaaat
    31921 ttccttgaag gagattgtaa ccggctggca cgttcggccg gtatggcaat ttcccaaaaa
    31981 ccaggcaata accccttcaa cccttttttc atatacggca actcgggact gggtaaaacc
    32041 cacctggcac aggctattgg cattgaagta aagcaaaagt ttccagagaa aaccgtcctt
    32101 tacgttaacg cagctaaatt tcaaacccaa tttgtagatg cccacataaa aaataacaac
    32161 attaacgact ttatccactt ttaccagagt attgacgtgc tcattctgga cgatgtgcat
    32221 gaatttgccg gaaaggaaaa aacccaggat gcctttttcc acatcttcaa ccacctgcac
    32281 cagaccggta agcagctagt actcacctcc gaccaacccc ccgctgagct acaggggctt
    32341 gagcagcgcc tgctctcccg ttttaaatgg ggactctcgg ccgacctaca aacacccgac
    32401 ttcgctaccc gtgtagcaat ccttaagcgt aaggtttata acgacggcat tgttatccct
    32461 gaagaggtaa ttgaatacat agcctccaat attacccaga acatccgcga gctggaggga
    32521 gcgctcatct ccatgcttgc acagtcaacc ctcaacggca gggaaattac cattgagctg
    32581 gcatcagaaa tgatagaaaa actggttaaa aaccctgcca gaaaggattt gtccatagac
    32641 tacatacaaa aggttgtgtg cgagtacttt ggtttaccag tagatgtgct ccaggcaaaa
    32701 acccgaaaac gagaaattgt gcaggctcgc cagattgcca tgtttttttc caaaaacctc
    32761 accaaatcct ccctctcaac aataggtgca gtgatcggca gtaaagatca tgcaacagtt
    32821 ctccatgcct gcaaaacaat taacaaccta atggagaccg acaaaagatt caaagcccag
    32881 attgaggata ttgagaaacg cataaagtat agcaaccagt aaagaaacaa cctaacaagc
    32941 agccctatac tacatcggct tgattaaaat tcacccagct gcattttaat ccaaataagg
    33001 ctatctttgt aaattgttgt agggtttacc agccgggaga aaagcttaag ttaattttaa
    33061 agctgatttc agcttgccat gaaagcatta aaaagggttg ctctacctgt aattgtagga
    33121 tttctggctg tgttgggcac tgcagctgct ccattggcta ccgatagttt agcccgaatt
    33181 ttaaaccagg ccggacaaga tactgcttca ttgaatgccg tttttaagaa gctgatttcc
    33241 gttgatttgc caaaccctac tcaaaccgat tccctcttaa acgaattact tagcaggttt
    33301 aagaagctta actacaacga tggcgtaaac cgggtgctat ctttcagggg agatctatac
    33361 taccagcagg gtaactttca gcaggcactt gaagttgaaa aagagctggc cagcaagtac
    33421 ggtgggtcaa agactaacat gctgccgctg gccaacgcct acaagatgat tgccgggtgt
    33481 ttttttaaca tgggtattca cgatagcgcc gttgtgtatg ccgagaaatc tatttcactt
    33541 ttcaaaaatc tgaacgatag ccaaaatgtt gcacgaagcc tcaatcttct tggaggtatt
    33601 tactggaaca tgggaaacct caccaaagct tccgaaaaac tatacgaatc gcttcggctg
    33661 cgcgaacaac tcaacgactc gctgggtatg gctaatgcat acaacaacat tggcctaatc
    33721 tacgactcac aagggaagct caccgaggca atgaaaatgt atgagagtgc tttggaaatt
    33781 tacaataaac tcaataacag caagggtatt ggtagggtat gcaacaacat ggccatcatc
    33841 ctaaaaaacc agaaaaaatt tagcgagagc cttgagatgt tcctaaaatc ccttgaggtg
    33901 gataaaaagt tgaacaacat tgacgaccag ggcaaaaccc tcaacaatat aggtcagctt
    33961 tacattgaaa tgggcaacat tcaggcggca attggatact accagcaagc caggcagcta
    34021 ttccttagga gcggaaatga aaacgggttg gctgcaacgc tcctaaacat ggggaaagct
    34081 tcctatgatc agaaaaatta cgctgaatca cgaacctact acctgcaaag cctggaagtt
    34141 gccaaacgaa taaattcaac cgaatggatt agagactcct atcatgggct ttacctgctt
    34201 gccaagcaag ctggacgtgc atcggaagcc cttactttat ttgaacgtta caaatcgctt
    34261 gatgactccc tgcgcgggct ggaaaatatg aataagatag accaactccg agttgaattt
    34321 gaaactgaga agaaagaacg tgaaattacc ttgctaacca aggataatga gcttaaaact
    34381 gaaaagctaa accgccagaa aacatacaac ctccttttac tcaccatcac aattgccgta
    34441 gtagcttttc ttgggctaac cgctttagcc ataaaacgtt taagagccga taaaaaaata
    34501 ttagtagata aaaaccagga aatacaagct cagaaggagg aaatagaatc tcaacgcgac
    34561 ctgcttgagg ttaccaacac agagctaaac cagcagaagg aagagctaca agcccaaacc
    34621 gaccaaatag agaggcaaaa ctacatcata gcatcggcaa accaaaggat gacagaaggt
    34681 ttggagtacg cctcgctaat acagaaatcc ctatcctctg acattaattc gtttggtaag
    34741 catttcagtg gatactccct gctctacatg cccaaggaca ttgtaagcgg agactttttc
    34801 tgggtgtggg atgaaaaccg tgatgataat tttacttttg ccattgccga ttgcaccgga
    34861 catggtgtag ccggtgcctt catgagcctg atggctataa atctgcttag ggatgccgtt
    34921 ggggtgaaca acattacgga gccacagaaa atctctacct tcctttacaa cgagctaatc
    34981 agaaaaaaag cctctatttt tgactccaac ctgatcatcg gactcgactt tattgtttgc
    35041 agctactccc ctaaaagcca agtactctgc tactcaggaa atcaccttaa cttctacctg
    35101 gtgcgagagg gggaggttaa caagctaaag gttgagagaa atattgacag acaatcggcc
    35161 accctgattt ttgcccagtc atccattaaa ctcaaaaaga atgacaggct ttacttcact
    35221 accgatggct ttgccgacca gattagcgaa accaaaagga aaaaaattgg aaattccgat
    35281 ttccagaatc ttctactctc agtaggtaac tatcccataa aacagcattc tgagttactc
    35341 gctgagtact tctcaaaatg gaagggtacc tacgaacagg ttgacgacgt attagttctg
    35401 gggttagagg tttaaacctc aaagcgggct ggattacgaa cgataaacca gggatttatc
    35461 agctcttctt tattgtagga cattctatct cccgaatcga acgaaattac ttgaaaccca
    35521 gcaccctcaa ctatagcctg accagcagca gtatcccact cagaggaaag cgataaacgc
    35581 ggataaatat cggctatccc ttcggctacc atacacattt taagagaaga cccacacgaa
    35641 atacgggtaa tgccaccgta ctttttatcg aggttcgaaa tgaattccag cgtttcgggc
    35701 gagttgtgcg accggctttc aaccacaaca ataccgctat gattttggct tagtggtagg
    35761 cgcttggccc cggagcacac atcagagtag gttaaattca cctccattgt tggcttgata
    35821 cctgtaacct taaacgaacc ctcggggtga atggaatagt aaaggcactc ggtaacagga
    35881 acatagataa cgcccaggat aggaaacccc tcatacatta aggcaatatt tacagtaaac
    35941 tctccgttgc gcttgataaa ctcctttgtt ccatccagag gatctacaat ccaaaagtac
    36001 tcccaaccct ttcgctccac atagtgaatg cttctaccct cctcgctaag aaccggtatt
    36061 aaagttttgt acaacgtatt ctttatttct tcgtgagcca ccctgtcggc caaggtgagg
    36121 ggtgttctat cagacttgag gttaacctgg aaatcatcgg agttataaac ctccatgatt
    36181 tttgaacctg ctcttacgct ggtatttata gccgtaacca gcaggtttat gatctctggc
    36241 tgttcaatca tgacctagtt gcttgctgca aaggtagctc actttccttg aataataaaa
    36301 aatataccct tccccaccaa ccccatttaa catttttatt cattaaaaaa taagcataaa
    36361 gaaacttcct tccaccgctt accaattcat gttttacaat atgtttgcta aggttcctca
    36421 aaattggaaa aattgttatg tttacccaaa attttaagac ataacaatac ctaactttta
    36481 acccatatca ccatgttaag ctcgcttgaa cccaacaacc tttggaaatt ctttgaggaa
    36541 atctgtaaaa ttccccgccc atcaaaaaag gaaaagctgg tcattgccta cctgcaaaat
    36601 tttgccaggg agcatggcct taaagtaaga accgaccatg ccggaaacgt gctagtagaa
    36661 aaacctgcct atcccggatt tgaaaaactc aagcccgtag tgctgcagtg ccacatggac
    36721 atggtttgcg aaaaaaatag cgatgtggcg cataactttc aaaccgaccc aattgtgcca
    36781 ggcattgacg gagaatgggt taaggccaat ggaaccacct tgggcgccga cgacggaata
    36841 gggatagccg cccagcttgc catactggaa tcaaccgaaa ttaagcatgg accgctggaa
    36901 tgccttttta ctgttgacga ggaaacaggt ttgacaggag ccaacgagct cgaaaagaat
    36961 ttttttagcg gtaaaattct gattaacctc gactcggagg atgagggaga actatttatt
    37021 gggtgtgccg gtggagttga tactctggct acctttgcct acgacactga aaaagtaccc
    37081 accaactcta ccgctttcag gctaaccgtg aaggggctaa agggagggca ttcaggtgac
    37141 gacattgaca agggcttggg caactctatt aagattatca accgttttct ctggcggggg
    37201 gttaacagct acgacctacg gctatccatg ctagatggtg gaaaccttag aaatgccatt
    37261 ccccgtgagg cctatgcaat ttttaccata cacgaggagg atagggctgc cctacgaaaa
    37321 gaatttacac tcatcaaaaa tgacattcta gccgagctcc atgatgttga acccgattta
    37381 tccataacct ttgaaaacgc cgacctacct ccctatgtgg ttgatgagcc cacccaatac
    37441 gacctgctaa acgcgctgta tgcctgccca catggggtta tttcgatgag taggcagctt
    37501 aaagggttgg ttgaaacatc taccaacctg gcttcggtga aatttataca ggacaaccag
    37561 atacttgtaa ccacaagcca gcgcagctca gtggagtcgg ccaaaaagga tatttccaac
    37621 atggttgaaa gtgttttccg gctggccaat gcccgaattc agcattccga tggctaccca
    37681 ggatggactc ccaacaccaa ctctgaaata ctgggcatta cccgcaactc gtacatcagg
    37741 ctttttggag tagaaccggt tgtaagggca atccatgccg gtctggaatg cggcttattt
    37801 cttgagaaat atcccgagct ggatatgatt tcgtttggcc caaccattaa agcggcacac
    37861 tctccctacg aaaggcttaa cattccttca accatgaagt tctggaagct tctgctggat
    37921 gtgcttcaaa atatcccgag tatttaggta gcctacccgc tatgtaactc ggtacttcaa
    37981 cgggaactac tcgggaagct catcaaaata ctctattgtt gcatagcaaa tagggcaatg
    38041 ccaactttca ggcagctctt caaaaggagt tccggcgggt atgcccgagg cagggtcgcc
    38101 ttcgtccgga ttatagatgt acccgcaaat tctacattta tacatcaacg cagggtattt
    38161 atgaattaaa gttgtgtggc aaatacagta aacccctaat ccttactgaa ataagcttgc
    38221 ataccagcat tcaaaatttt ctgaaaggag ttttcattaa cacgttaaat ggaggccata
    38281 cctaaacaat ttcgaccatt ttaaacggaa ccttaactag agcttgaaaa tcgcggccaa
    38341 gattcctaat ggtatcgtcg tcctcctcat agtaccagtt gaccaaaacg ctatggcctt
    38401 ttaaataggc gtcatcgagc atcttgataa ggttaaaaat gaagcgattc gagccgctgt
    38461 ttatgtactc cattctcagg ttaagcctgg taagtggttc gggacttttc aggtactcct
    38521 ccagccaccc aatcaccggt tgaaaaaatt caacggcatc ttctggtata gaccttcccg
    38581 aaacctgtag catgccaggc ttaaaatcaa tctgaggggt tttggatgta cccttaatat
    38641 aaaggctgct taattcggcc atggcagttt agcatttata tgttgaggta aatttagcta
    38701 aaaatatgcc cactttaaaa atttttggta aaaaaacgtc atcggattaa cccatcatcc
    38761 ggcataatcg ccaacattaa acagagtgaa cagttttcag ggaaaagctg tctttcctaa
    38821 acctaaggta acgaaaacat aatagtgatg aagccaggta atgcatacta tctggcttca
    38881 tcaagaatag acaaaagaat ttgcccacaa ctaaagggct acttgaggtt taacttgcgg
    38941 gcaatatcat cgggaatggc atttttatga accataatat cggtagtttt aagcagcaca
    39001 aagggtttag aagcatagaa aaaaccgtta tacttaccct ctgatcccca gctattcttc
    39061 accttgtaaa agatgttgcc gttctggtcc ttagccttgc ccataatcag cataccatga
    39121 tcatcggttg tttgatagtt atcaaaggcc tgctgacgca gctcctgggt aataaccatc
    39181 tcctttagag gtttctcaaa ggagtaaacc ttagcctcct tttccttttg ggagagttga
    39241 acccaatgat ccttatcgga acccgcattt tcagcgctgt ttatatcggg aaccactgcc
    39301 acgccattac gccagctaaa acccttttcg ctcacgtcgc taccccatgc aatggtgtaa
    39361 ccattatcaa ttgcgttatc taacaccctt tcaaattcgt taatgggcaa attataaacc
    39421 tcgccatgaa tccagttgtc aggaatttcc agaacaaact tgctgtagaa cgggtggtgc
    39481 gtgtacgaac caatacatac gtagtcatcg aggttaatcc caagcgattg tgcaaaagta
    39541 atgggtgtgt aagttttccc attgtaggta aacgatgcgg gaacctgccc aaggtagcca
    39601 tcaactatgc caatgaatgc gggtttccaa ccggtggaaa gttttttgtt gggattctta
    39661 actatctcat caacaaaaga acgggtaacc gcatcaagct caccatgaac atggttatcc
    39721 tctccgtagt tcaagccagg ataaacctcc atgggaacaa taccatactt ttttaccatc
    39781 tcaaccacgt cgaaaaacga acctccaggt ccgaaattat tggtgccgtt gaacctgaca
    39841 aagcgatcag ccttttcaac gtaagcattt ctcactacaa acatgggcga aaggttaact
    39901 tcgggtttac ccatgcgaag tagctccgat tccagaaagg caacacctga aaagctccag
    39961 caggtacttg agcgattctg gttcttaacc ggtgtaacct taagctcctt aattggggta
    40021 aaaacgtaac tttccgattc cttttgtggc tcctggccaa aaagcagctg gctaaaaagg
    40081 gccgttacaa taaaacacgc tgcaaataca gatcttttca tagtttcata cagtttaatg
    40141 ttcatgttaa acattgctta gctagtttaa tagattccgg atttaaaaaa aagtttaaaa
    40201 gtagccagac agtaaattac taacacgttt aaataagaaa cataacctcc ttaaaggcat
    40261 ataccctcaa ctcacccgta ggaagctgca agaccagctc ccccgtaggc tttacaccag
    40321 caattttagc cttaaagtta ccatttttgt cgcagtaccc atggtacaaa tctcgccggt
    40381 aaagcttctt taagtagtat tcgtccagct ggaaacggct ctgctcatcg gaaagcaatt
    40441 cgtaccgccg ggcaaaacat tccagaaatt ctgcgagcga ggagtgcaga tcgaactcca
    40501 tcccggtagc taaccttagc gaggttgggt tgggaatatc gtccgggaag gtttgctggt
    40561 tcaggtttat tccaatgcca accaccgatg tgcctagcat tgccgagcta aagctgttct
    40621 cgatcagaat gccggctacc ttcgaatcgc caatgtagat gtcgttaggc cacttaatgg
    40681 taacgtcatc gcccgaaacg taagcactca cccagtcgca cacggaagca gcagcaactt
    40741 ttgacaggta gaattgctca tcaaccctga gatgccctgg cctaagcagt atgctaaatg
    40801 tcaggtttct gccagcttcg ctctcccacg agttgccgcg ttgccctcgg cccatttcct
    40861 gaaacatggc ggcaataacc agcccttcag cagcaccctc ctcgcaacgt tgagcacaaa
    40921 tactattgga agaggttacg ctgttatgcc actcaactgt aaaatcaatc cttttcaagt
    40981 ttttcctgct ttaaatccag ctcctaaacc tacaatattt ttcatttggg tagattccaa
    41041 ctacataaat tcaacgacag ctgcttcagg gtaacatcca ttctatacag caaaggtttg
    41101 attagcttgc acaagtgcac atccccgtgt tggcaaattc cccggccaat agttgaaaaa
    41161 attaaaaagt tatcactaca tgccattccc tccaggtttt ggtcacccac aggttgatac
    41221 tgctggtttc aacttttttg cctaccttta aagccagctg ttgcaccaat ttagctccgt
    41281 ataaatcata taatatggct t
//
LOCUS       DAOSHM010000020        38632 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_819_length_38632_cov_20.155429, whole genome shotgun sequence.
ACCESSION   DAOSHM010000020 DAOSHM010000000
VERSION     DAOSHM010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 38632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38632
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_819_length_38632_cov_20.155429"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            312..3038
                     /gene="ppdK"
                     /locus_tag="PK839_04885"
     CDS             312..3038
                     /gene="ppdK"
                     /locus_tag="PK839_04885"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008673882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HOG72107.1"
                     /translation="MSKVKRVYTFGNKKAEGKADMKNLLGGKGANLAEMCLLGLPVPA
                     GFTITTDTCNEYYQNNHELPSDLIPDIWEAMKNIEKIMGMKFGDPENPLLVSCRSGAR
                     KSMPGMMDTVLNIGLCSSTIPGLIKKTNNPRFVWDSYRRLIMMYADVVMEKAEGVEPT
                     DGKGIRKLLDDILDEYKNSKGYKDDTQLTAEDLQHLAEEFKKQVKKSLGKEFPDDAKE
                     QLLGGIKAVFKSWNGKKAVSYRRIEGIPDDWGTAVNVQAMVFGNMGNSSATGVAFTRN
                     PATGENVFYGEWLINAQGEDVVAGIRTPNPLNNETKNDQNKHLPSMEELMPDTYKELV
                     AVRNTLEKSFKDMQDIEFTIQEGKLYMLQCRTGKRTGTAALNMAMDMLHEGLIDEKTV
                     VLRVEPAQLDELLHPICDPEEEKKVNPIVKGLPAGPGAAVGKVVFTAEDAVAAARRGE
                     SVILVREETNPEDVEGMRAAVGILTARGGMTSHAALVARGWGKCCIVGAGAMLVNSSE
                     KIAKISGTNIVIKEGDTLTLNGTRGHVYSNALKLMEATENPRFREFMKLVDKYRKMGV
                     RTNADTPEDAKIAREFGAEGIGLFRTEHMFYGKGSEEPLFLLRKMILSTTVEERRKAL
                     DELFIFVKRDIKNTLVAMDTLPVTFRLLDPPLHEFVPQSPEKQAELAKSLGITVEAVA
                     KRGENLHEVNPMMGHRGVRLGITYPEVTEMQVRAVFEATAELLKEGKSPHPEIMVPVT
                     CEVAELDFTRKIVDRVYAEVCQKFGMKSIEFKYGTMIEIPRACLLADEIARTTEFFSF
                     GTNDLTQMTFGFSRDDAGGFLPDYLDKKLLPADPFQTIDQKGVGQLIQMAVEKGRSTR
                     PELKVGICGEQGGDPASVEFCFKTGLTYVSCSPFRVPIARLAASQASIKYGK"
     gene            3099..4520
                     /locus_tag="PK839_04890"
     CDS             3099..4520
                     /locus_tag="PK839_04890"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HOG72108.1"
                     /translation="MSRQKSKLIRVIIQLFLLILPATWLWAQDDELLPTDTADFKSHY
                     NMLLIFEADSGHTDMVQYLLELGADPNTQTEQGVTPLMYASQWGYRDIAELLLKNGAN
                     PNLAPFDGNTALHASVKAGNDSIAELLLDYNAEVNVANRKGLTPLHYAVWYGFPYLTD
                     LLIYHGADVNAQDNHGNTPLMLAVYNGTNLCTTLLLESGANPDIQDNEGKSPLMVAAQ
                     FNDTLLSKLLLSYNADIKLHDKKNATALALAIANRSSQTIQLLLENNAGSYTLSKSYY
                     QLAAETGSREVLEILRKNGLKTKLKPSIGSIGVGTGFIFNNHEFLWGFEIEPVESISK
                     TGLSVGYWFRPSPVAVLYPKNNKLFQLHEEKQVVSVKLRHYIPIVFRPKGIGLKAQFG
                     PSFDWISRNTSGTVTNPPDVVRLGANAALNLHINSFEITVGWDYTWLKTLDASPHRAN
                     INIRYNLPIAKPRIVNKTIKYVR"
     gene            4510..4935
                     /locus_tag="PK839_04895"
     CDS             4510..4935
                     /locus_tag="PK839_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72109.1"
                     /translation="MSGRLNKGLIFIVLLAAIPLSCEELNSWFVDCSQCFKDKISRTD
                     ITVKLTINDENPWVIFDVYRGNTDSGELLFTDTSVVAEYYWRAEINQFYSVKARYSCK
                     GRVIYVVDGAMLKTKLESSACDEKCYTIHGDVLDARLKY"
     gene            5248..7443
                     /locus_tag="PK839_04900"
     CDS             5248..7443
                     /locus_tag="PK839_04900"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HOG72110.1"
                     /translation="MKRNLVKLLLIAVITRFGVWSALADEGMWMVNLIHQNIATMQTM
                     GLKLTAEDIYSLNHSSLKDAIVQFNDGGCTGELVSAQGMFFTNHHCGVEAVQALSSEK
                     NNLLENGYWAGSLSEELPAHDQVALILVGVEDVTAKVFASLNAGLSNQEYFDKMREVM
                     QQLEDETSKKTGYHTIVKPFYNYNQFFMFTYERFLDVRLVGVPPSSIGNFGGDVDNWH
                     WPRHTGDFCIFRIYTAPDGKPAAYSPRNVPYKPKNFLKISLKGVNEGDFAMIMGYPGT
                     TYRYATSYEAQYSRDVVAPWKVDVWGNFISIVKQARNYNPKAKVAYTDKHDYLVNFYQ
                     KDVWQAQSMFRYNVVERLAAREDSLKAWVNQNPSLRSKYLPTIPVTRNFFEQATQNKW
                     EALENSLSALSFFPVDVYKNVNACNNLLGAIFEQNKPYSPLKFWKVDRIRREARVLKK
                     NIPGIFDSYFYEPDMRLYATAFGNLIANIGDCQNLGLLNAIKKVDNIDSLYPYYIQGF
                     YEKSYFTKAQNLEKLIKYPYMDSLLTDPLFILYHNYSALWDSIYPQMHNARMDFQKAM
                     QLYTKGLMEMNAGKLLYPDANSTMRLTYGRVTGYKPADGLYYKPFTTLDGVMEKENPN
                     EDVFTVDPKLRQLWQNKDYGRYASNGTIPVCFLTDNDITGGNSGSPVLDGNGNLIGLA
                     FDGNSEAMACDFMFEPETQRTIAVDIRYVLFIVDKFANAQHIIGELTTL"
     gene            complement(7525..8286)
                     /locus_tag="PK839_04905"
     CDS             complement(7525..8286)
                     /locus_tag="PK839_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOG72111.1"
                     /translation="MKITFLGTGTAQGIPVIACSCPVCASTDSHDKRLRTSALIEEQG
                     INILLDAGPDFRQQMLNANVKNLNAILITHEHRDHVAGLDDVRAFNWVQRQPMDIWAE
                     DRVLEAIQREYAYIFAERKYPGIPEVELHQVFGHQFDILGVTVIPIRVYHCKLPIFGF
                     RIGDLTYITDGSFISEEEKDKIVGSRVLVINALRMQKSLSHFSLPEALKLVEELSPRK
                     AYITHISHQMGFYRDVARQLPDNVFLAYDGLVVEV"
     gene            8470..9177
                     /locus_tag="PK839_04910"
     CDS             8470..9177
                     /locus_tag="PK839_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002559375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOG72112.1"
                     /translation="MAGKLYLVPTTIGSTSWNEVIPSGVVELTRGLKFFVVEELRTAR
                     RYLSALGIQTPIEKLCFFTLNEHSSAADLTDMLKPLLDGNDVGLMSESGLPAIADPGS
                     MLVSAAHHHGIRVVPLCGPSSIIMTMMASGLNGQNFAFVGYLPVKSEERKRRIRQLEQ
                     RSRAENQTQLFIEAPYRNVALFRDILQTCADEARLTIAAGLTLEGEYIATKKIREWKQ
                     LPIPDINKINTVFALLF"
     gene            complement(9169..10179)
                     /locus_tag="PK839_04915"
     CDS             complement(9169..10179)
                     /locus_tag="PK839_04915"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOG72113.1"
                     /translation="MRKIFFLLISVFILALALVACDRDFSDKEGDELAMLEAWVQVNG
                     IPTESLKPSGLYFVNQQEGTGLSPSEGDFVIYSYKEKSLDGIVLSTNDSATAVLYGEY
                     NVNVHYSQLFTKYDKSNSTVEGYQREMVKGVYEGLGYMKEGGKAMLIMPSKLAYGKRS
                     VSYTSSVIRPYQSLVYEVELHKVVKNPREYELQLLHDYMNLHYPGLAPVNDSIYYIQL
                     SPPTNDTVTLAKDSVAYVYYKGMLLDGYMFDSNIDSVATRLGRSFSSTDSLKVTVGTG
                     VIKGFSQALVLMKKGEWGRVIMPSYCGYDSVGSTTIPPYSPLIFDLYFSSKSVVTKPQ
                     SK"
     assembly_gap    10277..10376
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(10707..11603)
                     /locus_tag="PK839_04920"
     CDS             complement(10707..11603)
                     /locus_tag="PK839_04920"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOG72114.1"
                     /translation="MIQKNFFNAILLLSPLLMGACDNSLKEDKRNDEEKIIEGLISSK
                     KWTYTKSEGVYHVVVQPSYGYEVNYGDTVTFWYKGYTIQSPVLVFDTNIKSEAVLAKL
                     DTNTRTLEPVRVIAGKTKLIEGLKRGLLLCRQGQKSTIFFTSDIGFGDNQVGPLPPWT
                     PLAYDIEVVALNGRGIESEQNIIKSMDLTGYTLHSSGLYYRYLLDSSTVRPLSNSVVY
                     GWYSISLTDGTVVTQTPGANEKVDLTGQMPEALRLGFQLASLGGKITILAPSPLGYGK
                     SGSSKVMPYVPIVVDIRLDSIK"
     gene            complement(11600..12487)
                     /locus_tag="PK839_04925"
     CDS             complement(11600..12487)
                     /locus_tag="PK839_04925"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOG72115.1"
                     /translation="MTKSSGILLAFLVVATVGCSNRSKLFPGFSVTPTGIHYKLISLG
                     NGTSTAKPSNYITASISYRTAKDSVFFQGTRQFQLAQSSYEGSIDECFMMLAEGDSGA
                     FYIKALPFFTQTLETKLPTFIGVNDYMRVDIRLHSIKTQEEFKKDMEAFLSWIEDFGD
                     YEKVIIRQYIDGQKLSVEPTPSGLYIIPQIRTERAPVEKGDTITIHYEGRFFDGRIFD
                     STRKRNEPFQLVYGDKWQVIPGIDEALGTMREGEKSMLIVPSHLAFGQQGNTSGIIPP
                     FTSLVFELEVVEVKKGVQL"
     gene            complement(12534..13058)
                     /locus_tag="PK839_04930"
     CDS             complement(12534..13058)
                     /locus_tag="PK839_04930"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOG72116.1"
                     /translation="MKILTIIPLAVILVSCGGNERQPDRQPNVAETREMLQRINKVLV
                     QQDREQILDYIKRNNLVEMKESPTGLFYYISGTSNGPKVRKGDVVEYSYRVSLLDGTV
                     CYQSNAEAPATFEVGHGGVVSGLEEGILLMRQGQSAKFIMPPHLAYGLIGDSKQVPAR
                     SIVIYDVELLAVYR"
     gene            complement(13093..14127)
                     /locus_tag="PK839_04935"
     CDS             complement(13093..14127)
                     /locus_tag="PK839_04935"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HOG72117.1"
                     /translation="MKLNLDEAWAGALRELLVPTNRIVLTTHHNPDGDAVGSVLGLWH
                     ILEQVGVHATAVTPNDFPDFLSWMPGSDTILRFSSSRQKVREVMAGADIIICLDYNGF
                     SRTEDMESLFSASSAKKILIDHHPQPENTFDLSISVVEVSSTSELVFELATEIYGLKA
                     LNADAATCLYAGIMTDTGSFSYACSRGRTLEIAGMLVDSGAVVERIQSLVYNNFSADR
                     MKLLGYCLADKMMVFPEFHAAYIWLTREELKRFNYQMGDTEGLVNYPLSIKGIIFSVI
                     FVENDNHIKVSLRSRGNFSVNEFARKYYNGGGHLNAAGGKSFQPIDATLADFEQLLRQ
                     HLQELNAAAI"
     gene            14426..14845
                     /gene="ndk"
                     /locus_tag="PK839_04940"
     CDS             14426..14845
                     /gene="ndk"
                     /locus_tag="PK839_04940"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: protein motif:HMM:NF001908.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HOG72118.1"
                     /translation="MNTRFTFCMIKPDAFKNDCVSEILCEILKAGFKVRGIKITMLTK
                     AKAEQFYDIHRGKDFFDKLTTYMSSGPVMAMVLEKPEAVEALRKLVGKTNPEEADEGT
                     IRRRFGVNTTQNAVHASDSYENAEHEWGFFFSKREIY"
     gene            15285..18752
                     /locus_tag="PK839_04945"
     CDS             15285..18752
                     /locus_tag="PK839_04945"
                     /inference="COORDINATES: protein motif:HMM:TIGR03296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M6 family metalloprotease domain-containing
                     protein"
                     /protein_id="HOG72119.1"
                     /translation="MKKTLLSFLGVCLMAAAMVTNQAVAVPAYPGLIKYEQANGKIIN
                     IYLKGDEKVKWVLSEDGYTLMLNSKGVYEYAFQDGKGDITLSGVEANNPAERTKAERE
                     FLGKVSKGLYYSPSQLSLMKSIWEIKSAESLKAFPTTGNRKLICILMGFKDKAFTKTQ
                     SDFNNLFNQVGYTTGGATGSVKDFYMENSWSQFNLTVDVFGPYTSSQNMSYYGANDAS
                     GYDVNPRALVTEAVNAADASANYANYDNDGDGTVDGVYVIYAGYGEEAGASADCIWAH
                     AWSITPVTKDGKTISKYSCSSELRGTSGTTITAIGVICHEFGHVLGAPDYYDTDYSTG
                     GQYEGTGYWDLMASGSWNNNGVTPAHHNAYTKVKVYGWATATVLSSAATISVTNAEAN
                     KSFYQINSTTTNEYWIMENRQQVGFDANIPGHGLLIYHVHKDIATASSSNNINATYPQ
                     KMYPVCASATTNPGSTPSSYGSISSTGCPWPGSSSKTSFTDATTPNMKSWAGANTAKP
                     MTNIAENTSTKTVTFDFMGGSGGGTAPTATTLAASAISTSSATLNGQVNANNATTTVT
                     FEWGTTTSLGSSFSATPSSVTGTTTTNVTGNLTGLSANTTYYFRVKAVNASGTTYGST
                     LNFTTSSTPTSITLPVTENFNSSTLPTGWTTQNTGTSITERWSVSNTYKAGGSAYEMK
                     CTYQNVNPGTTRLITPAINTVGVSQVTLTFKHFLDAYSTGATLRVQTSNNKSTWTNTT
                     WSVATGSSNVGPATVTVNITSNLNSETTYIAFVVDGNLYQIDYWYVDNVSITAGSGAT
                     IPTVTTNSVTSITSSSASCGGNVTSDGGATVTERGICYSTSQNPTTSSSKVTSGTGTG
                     SFTANMTGLSASTTYYVRAYAINAQGTAYGAQQSFTTLSGSTSLPLPVTQTFNTSSLP
                     TGWTTQNTGTSITERWSVSNTYKAGGSAYEMKCTYQNVNPGTTRLITPAINTVGVSQV
                     TLTFKHFLDAYSTGATLRVQTSNNKSTWTNTTWSVATGSSNVGPATVTVNITSNLNSE
                     TTYIAFVVDGNLYNIDYWYVDNVSIVASGSGSPAFDTPISDLTENDGVKIFNLYPNPA
                     SDYINISLTGASSEVTVRIFDVRGSMVKSQVLNASENRIDVSTLAKGIYMVSVGEGKE
                     ISTKRFIKQ"
     gene            complement(19058..20236)
                     /locus_tag="PK839_04950"
     CDS             complement(19058..20236)
                     /locus_tag="PK839_04950"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOG72120.1"
                     /translation="MRRISLIISNLLLFVAIANAQVSIEKILGEVERNNTTLVALRQK
                     MEAEKIGSRTGLYPQNPSFEYSYQWGNPADIGSKTDISLIQSFDFPTAYLYRSKIAKA
                     EIELLATEYLRQSKQLLLDVRLVCIELVYQNALSSVLGKRVSNAKLIADAYSKKLDVG
                     EASALENNKARLTLLNATQELFSVKLKQNELLAKLASLNGGIPLELSDSVFNMPAIPA
                     SFDEWYAATEQSNPDLKAIEREIAVAQRNIQLSRALCLPQIKAGYASEKVAGEHFQGI
                     AAGISIPLWENSKRVKYAKARAQYLRSVEADRKVEIFSRQQAQYNTALELQKNIAEYR
                     NSMGTINSSRMLDEALQKGEISLVDYLLEESLYYSSAETLLQMERDFCRVVAELYQYQ
                     "
     gene            complement(20233..23334)
                     /locus_tag="PK839_04955"
     CDS             complement(20233..23334)
                     /locus_tag="PK839_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005933348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOG72121.1"
                     /translation="MLDRIIKFSLSNKYTILIAFFVLAVVGIRTARNMDVDVFPDLTA
                     PTVVVMTDAHGMASEEVERLVTFPIETAINGATDVRRVRSVSSQGFSFVWVEFDWGSD
                     IFKARQIVSEKLIAVSSQLPIGVGQPVLAPQSSVMGEIFFVGLTADSTSMMDLRTIAE
                     WEVKPIILATNGVSQVTIIGGDFKQYQVLADPVKMMSYGVSMDELATVCRNISGNSTG
                     SNIRQFGNEYAVRGMARASDLETLGNSFIKFLNGHPVRVSDVAEVVIGSAAKMGYASE
                     NTKPAVIISISKQPNANTLKVTENIEKNLDNIYKTLPKDVRLDTGIFRQADFIQTSVG
                     NVQNALMEGAIFVVIVLFLFLGSFRTTIISLLAIPLSLLGTIVALKAFGLTINTMSLG
                     GMAIAIGSLVDDAIIDVENVFKRLRQNNLKPVDERQSSFTVVFEASREIRASILNATL
                     IIIVAFLPLFFLSGMEGRMLKPLGISFIVSLTVSLVVAMTLTPLLSKMLLTNESYLAK
                     NEREKWLSRNLQTYFKASLEWVLRHKKVVLGAVLSLVILSIVAFASMGRSFLPEFNEG
                     SLTLSVVTKPGTSLDETNKLGNLVEQQLLAIPEIYSTARRTGRGELDEHSQTVNSSEI
                     DVNFKLSERSRDEFVANVRQTLAGIPGIAATVGQPLGHRIDHMLSGTRANIAIKLFGT
                     DLNQMFAIGNQIKGEISGIEGLVDVNVDQQVDIPQIQLKANRDMLAAYGIPVGSFNEF
                     IDVAFGGEKLADIYEGQRRFDLVLKLNHRYTESIEGIGVALIDTYNGGKVPLSEVAQI
                     QSVSGPSSISRENAQRKVVVSANVAGRDMRSVVNDIQDRVNSHITLPEGYRIEYGGQF
                     ESEANASRTLMITSILAILVIFLLLFQEFRNFKLAAIILLNLPLALIGGIFSIYITGG
                     ILSIPAIIGFITLFGIATRNGILLVSKFQQLQAQGLPIHDTILKGSTDRLNAILMTAL
                     SAALALIPLAMRGHLPGNEIQSPMAKVILGGLITSTILNIYIVPIAYSLLAKRGLTKT
                     DKQ"
     gene            complement(23346..24872)
                     /locus_tag="PK839_04960"
     CDS             complement(23346..24872)
                     /locus_tag="PK839_04960"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOG72122.1"
                     /translation="MKRLFILIFSITIYGCSHTADSSAGAAHQHDESSHEAEAGDDAQ
                     TFTIYTSTCELFAEAKPFVVGTTTTVLSHFTNLADFKPLTSAAITIKLTVNGKEYSQT
                     IAIPEKNGIYHFTVTPDLSGQGQLVWIVERDGKSELFDAGLVRVFANEHEAEAFHAEH
                     IPVDAIRFTKEQSWQVDFSTEWPCMEAIGEVIKTTARVEPAPTDEMVISAKTGGIVSF
                     YSSKPLVLGQHVERGDKLLEIVSSGLANEDLSVRYAEAKSNFERAKSNYERILELSKS
                     KVASEKELGIALNEYQNASSTYTNLKENFSASGQNVSSPIDGYVQDISVQNGEYVEAG
                     QPVLTVFGSRFYVLNAGVQQKYFKSLEGLKTATVKQANGSYVGIDKFNGRIMSVGNSA
                     LAGNGYLIPVIIQVDATDGLVVGSFVELNLIAKSDSMLLTVPTLAILEEQGNYYVLVQ
                     LNPETFEKRQVQLGVSDGLRVEIRSGLLSTERVVTRGAVLVKLAQVSGKLDAHAGHVH
                     "
     gene            complement(25096..25533)
                     /locus_tag="PK839_04965"
     CDS             complement(25096..25533)
                     /locus_tag="PK839_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72123.1"
                     /translation="MGVKQLCSVWLILLANIMLQVHAFVPHHHHNGQVCFDGYSVNIT
                     IPNDGNDARNNPAHPTDEDAECCALKHDYLIPDDGFEYKPAVTNIHHGFNIAGGFITS
                     CASIPFQIFLVNTRLYGGYTNNFHFSSYVSIPGLRSPPAKNLL"
     gene            complement(25666..26883)
                     /locus_tag="PK839_04970"
     CDS             complement(25666..26883)
                     /locus_tag="PK839_04970"
                     /inference="COORDINATES: protein motif:HMM:NF019301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HOG72124.1"
                     /translation="MKQVVIISRYLLAVVFIFSGFVKGIDPMGSAYKFHDYFMAFHMN
                     FMQPLSLAFAFILCAAELLIGLMLLYGVNLRIAAWGVFIFMLAFTPLTLILAIFNPVS
                     DCGCFGDALHLTNWQTFFKNIVLLSAAYTFFRYRASLPPSFGEKKQSAMFAVLCVVAF
                     LPPIHGSLHEPLYDFRPFHVGVNIHQAMIYPDGAPADVYKTKLYYKKNGEVKEFDESN
                     YPWQDSSWTFVDSKFILVKKGYTPPISNFSLHSVDGVDLTDTIIQSTGYTFLAVSPQL
                     EKAGVKAQEQVKNIFMGATERGYKFYWATASSGEAMEKIKVNSRVELPFVLGDEVTLK
                     TITRSNPGLILIYNGTIIGKWGWRDIPEQLFKNPDLLAWQLNNQRLIAGNRLTATLVL
                     MLMLAFIALKPWK"
     gene            complement(26909..27601)
                     /gene="tmk"
                     /locus_tag="PK839_04975"
     CDS             complement(26909..27601)
                     /gene="tmk"
                     /locus_tag="PK839_04975"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019129850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HOG72125.1"
                     /translation="MALIVIEGLDGAGKSTQINLLGSFLTQKGVASHFLHFPRVDAPY
                     FGELIARFLRGDLGRIDEVDPYVVAMLYALDRMDASGLIKSWLKTDEVVLLDRYVYSN
                     IAFQCAKLNDSSRVDKLRSWILNLEFDYFAIPKPDISIFLDVPFEFTSKRLSEQREGA
                     DREYLNGRDDIHEKDLNFQQRVREVYLLQQSLDPQFVVIPCYDEHGSMLEPDKIFNRI
                     VEAILVHKALVI"
     gene            27704..28585
                     /gene="folP"
                     /locus_tag="PK839_04980"
     CDS             27704..28585
                     /gene="folP"
                     /locus_tag="PK839_04980"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007660571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOG72126.1"
                     /translation="MEQPPSYLKQRKTMTSNGRIISLDKPLVMGIINVTDDSFYNGSR
                     YKLGFRIAARAEQIIREGGSIIDVGACSTRPGAKPVSEQQELKRLAKALHAIRKKYPE
                     AIISVDTYRSRVARMAVGDYRANIINDISAGELDPEMFSTIAELKIPYVLMHMRGTPE
                     TMQQNPTYDNVIREIITYLNGKIEQLKQLGVSDIIVDPGFGFGKTVDHNFSILKNLQV
                     FRLFERPILVGLSRKSMISKTLGIPAAKCLNGTTVLNTMALLNGATILRVHDVREATE
                     AIKLVESTTRQPDYQYI"
     gene            28661..29446
                     /gene="cdaA"
                     /locus_tag="PK839_04985"
     CDS             28661..29446
                     /gene="cdaA"
                     /locus_tag="PK839_04985"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HOG72127.1"
                     /translation="MTNLFITIRALDVIDVVLVAFLLYQMYMLIRGTVAINIFVGIVL
                     LYIFWLVVRALNMELLSSILGQIIGVGVLALIIVFQQEIRKFLLFIGNRYINRRRFSF
                     ENFFFPKKREKQILVKVDAIVKACRNMSETRTGALIVIARKSELQLYAESGDIIDADT
                     STRMIENIFFKNSPLHDGAVIIVNDRILAARCVLPITENINLPPHYGMRHKSAIGITE
                     VTDAIAIVVSEETGKISVASEGMLRYDIGWLELDKYLRDNLSS"
     gene            29550..30797
                     /locus_tag="PK839_04990"
     CDS             29550..30797
                     /locus_tag="PK839_04990"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72128.1"
                     /translation="MIKYAISKADQNLKGEITLSPSKPMNHKFLVIRILKSSNLTQRL
                     TTESEDAQIVNSEVINEGIKKNRGTSAKALRHIRAFLNYFGGEWILSSSNILKDRSTE
                     RIVKVLQKSGLNVSYEKRSGRPPFRLTGKNLSGIIQRVDGSINSKIIESKLLFAPTAS
                     IDQIEEVCNRILSSNYVGTTLRALQYLGVNTDWKEDEVLVEHEINDGSEMVIEPDWSL
                     ASCWYQMVALAQKGELQINGLKIDTFQNESAIRHIYNDFGISTLIMPDGISIKRKGKL
                     TGKFTHDFTSYPNLLPCAITTCVGLGIPFEMKGIESLNAADPNRMQLLTEELVKVGAK
                     VELKSINGTQVATFNGTSGLSRLKSVEFESKNDYRILLALVSIAVRGIQVIIDSPMAT
                     NKAYPSFWDDMKKLGFVVKSVTE"
     gene            31194..32045
                     /locus_tag="PK839_04995"
     CDS             31194..32045
                     /locus_tag="PK839_04995"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HOG72129.1"
                     /translation="MRNKKVLRLGLLGLLATMMVASCSKEEDERLQEPISNDWTVRPD
                     FATLDTRPQDVIASFTLTEKDAELVKPDENVVKGAKYALVIGISDYSGTQNDLQYCDD
                     DATDWKNRLVSEGYSVTILLDRNATKSAIESAVNSLASKSIAGNEIAFCYSGHGSRGN
                     IVTTDLYYISSSWFKTKFSTAKSTKMMFCFDACQIGAMKTALNATGRVIAVASSTSTY
                     SYDGDASMRNGVFTYYQMYGFDNLSYTYLEPDCSYACSSMKSWASARRLKVAPSYADS
                     YSGNFDL"
     gene            complement(32165..33316)
                     /locus_tag="PK839_05000"
     CDS             complement(32165..33316)
                     /locus_tag="PK839_05000"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HOG72130.1"
                     /translation="MLLIRNLPLYSSLFLLLSFAGAARGQAVFLLDSLPAKTSATDTV
                     FMATSLNGWNPHDLMYAFSPDSLGTRILQLPSVVDTFEYKLCRGSWKTVEVSADGRDI
                     PNRLYTSYDTVKLSVGGWRDMLAKGLAVSTASENVRYLPTSMRMPQLNRNRTIRLYLP
                     PNYADGGPFPVIYMHDGQNLFDDATSFAGEWHVDEILDSLYTYRGFSAIVVAIYNDDS
                     ERLNEYSPWRNDSLGAGGDGNKYVRFIVETLKPFIDKHYRTDPRSQSTAIIGSSMGGL
                     ITLYAALEYPNVFGKAAVFSPSLWFSPKVFEYIQKYRRKGDQRFYMLAGEKESERMVT
                     DVTRAADLLRAAGFNNDSYLMIKIVPNGRHTEGFWSRELGEAIRYLYEF"
     gene            complement(33369..34322)
                     /gene="metF"
                     /locus_tag="PK839_05005"
     CDS             complement(33369..34322)
                     /gene="metF"
                     /locus_tag="PK839_05005"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009597798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HOG72131.1"
                     /translation="MKVIDILTKSRSTLFSFELLPPLKGHTIDTVFDTIDRLKEFSPA
                     YINITHHREDVVYMPNTAGEPVPVKVRRRPGTISLAAAIHQRYGIDVVPHLICGGFDA
                     HDIEDALIDLDFLGIQNILVLRGDADKISGRFEPKRGGHQHAVELVRQVQEFNRGIYL
                     EKLIENPKESDFCIGVGGYPEKHPEAPNIDVDIRYLKEKVDAGASYVVTQMFFDNAVY
                     FKFVERCREAGIDVPIIPGIKPISAKEQLTNLPRVFNVNIPQVLVQEVDRCADNRQVF
                     DVGVEWAVAQSKELKARGAPAIHFYTMGRAENIARVAAKVF"
     gene            complement(34439..38110)
                     /gene="metH"
                     /locus_tag="PK839_05010"
     CDS             complement(34439..38110)
                     /gene="metH"
                     /locus_tag="PK839_05010"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HOG72132.1"
                     /translation="MKSKELIAEQLNKRILVLDGAMGTMIQQLGLTEEDYRGAVFANH
                     PVNLKGNNDVLALSRPDVIASLHKQYLEAGADIIETNTFNANAVSQADYALEDWIEQI
                     NLEAAGIARRLADDFTTLSPDKPRFVAGSIGPTNRTASMSPDVNRPGYRAVTFDTLVQ
                     AYSLQIKALIKGGVDLLLVETVFDTLNAKAALFAAASVMEELQTDIPVMISATIADLS
                     GRLLTGQTLEAFVTSVSHYPLLSIGLNCAFGAEQLLPYVKQLAVVTPFRVSVHPNAGL
                     PNQLGGYDQSAEMMAGWVEQMVQAGAVNIVGGCCGTTPKHIERVAAVASKYPPRKTPT
                     LPRFTRLSGLEMLEIRPESNFINIGERTNVAGSKKFEKLIIEEKYGEALAIAREQVEG
                     GAQAIDVCMDGALINAPVAITEFLNLVASEPDIARVPVMIDSSRFEVIEAGLKCTQGK
                     SIVNSISLKEGEQEFIRRAKLIRRYGAAVVVMLFDEEGQATTTEHRCRVAMRSYNLLV
                     QTVGFAPEDIIIDPNILAIGTGMAEHSSQAVSFIETTKWIKQNLPYAGVSGGVSNLSF
                     AFRGNNQIREAIHTVFLYHAVNAGMNMGIVNPGMLRIYTDIEPDLLRLTEDLVLNRRH
                     DATERLLAYAHNVKDDKTDDKALPEWRSYPPGKRLEHAIIKGNEDYVEVDTLEAFSIM
                     GSAISVIEGPLMDGMKEVGTLFGSGRMFLPQVVKSARVMKKAVGILEPYISSSDESSK
                     IQKSKGTIVLATVKGDVHDIGKNIVSVVLACMGYEVVDLGVMVPTDKIINGVIGHKAD
                     ILGLSGLITPSLEEMANVARELEHSGLTIPLLVGGATTSELHTALKIDTEYSGSVIHV
                     KDASLAGGVVAELLSPNQHEMYTQKVKERYAGLRSTYSEQKKALISIDDARQNRFKFD
                     WSSYTPPQPNHRGIQPFLNVDISELRPYISWTFFFHAWGLKGRFPEILTDPVTGSEAK
                     KLLDEANRMLDDIAAAKSIRASGVAGLFPAASQNDDILVFTDETRSSVMATLPQLRNQ
                     QLKTNGEFNLSLADYLAPLNSGIPDWLGCFVVTSGIGVDELANKYRAAGDDYKAIMVK
                     LLADRLAEAFAEKLHQQVRKELWGYRPNENLSPAEVLAGRYDGIRPAPGYPACPDHRG
                     KKFIFDLLKASDLLGVELTETLVMQPLASVCGYYFSHPKSSYFNIGRVTNDQLGDYAR
                     RLGVSLEEAVRFFPTNVEQ"
     gene            complement(38141..>38632)
                     /locus_tag="PK839_05015"
     CDS             complement(38141..>38632)
                     /locus_tag="PK839_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014776560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HOG72133.1"
                     /translation="LVAHLRKFSESCFTLFVIPTLVAPDDYIFNVENEFNGVVIEGQY
                     YDKQFMFGKGAGGFPTASAVLSDITARLHGYRYEYKKFYFSSPPSYTTDVVLSLYIRY
                     SRESDLNVFDILEVQERYNGPEYRWVVARVKLSSLLKIKELLPKRNLFTIFTGEYSGG
                     ILS"
BASE COUNT        10990 a         9292 c         8445 g         9805 t
ORIGIN      
        1 aacttgtccg cctaccggcg gattctccac tcaacaggtg ttacagtgag tctgccgaac
       61 tgctcactgt aacacctgtc gaacttttca gggttagttt ttagctgctc tcaacctctc
      121 ggcttacaag caagcttaca tattcaccta cccgatagcc ggcccattca aacgattgca
      181 agaaaatgtt gcgctcaaaa ggaaacagaa aatcacgtcc aaaatttttt aatacttttg
      241 taagttgtct aagattttca atttgtgctt cttatacata actaaaagtc taattaaaac
      301 ctgattgata aatgtcgaaa gttaagagag tttatacctt tggtaacaaa aaagctgaag
      361 gtaaggcaga tatgaaaaat ctgctgggag gaaagggggc aaatctggcc gaaatgtgcc
      421 tactagggct tccagttcct gcagggttta ccataactac agatacttgt aacgagtact
      481 accagaataa ccatgagcta ccctctgacc tcattcccga tatatgggag gccatgaaga
      541 atatcgaaaa aatcatggga atgaagtttg gcgatcccga aaatccgttg cttgtcagct
      601 gccgctcagg tgccaggaag tcaatgcccg ggatgatgga cacggtgcta aatataggcc
      661 tttgctcgtc aaccatccct ggattgatta aaaaaacaaa taatccaagg ttcgtatggg
      721 attcttaccg ccggcttatt atgatgtacg ctgatgtggt aatggaaaag gctgaaggtg
      781 ttgagcctac cgacggaaag ggcatacgaa agcttctcga cgatatactt gatgagtaca
      841 agaatagcaa gggttacaag gacgataccc aactcaccgc agaagacctt caacatctgg
      901 ctgaagaatt taaaaagcag gtaaagaaat cgttgggtaa ggaattccca gatgatgcca
      961 aggagcagct gctgggcgga attaaagctg tttttaagag ctggaacggt aagaaggccg
     1021 tttcgtaccg caggattgag ggaattccag acgactgggg aacagcagta aacgttcagg
     1081 ccatggtatt tgggaacatg ggcaacagct cagcaaccgg tgttgccttc acccgcaacc
     1141 ccgcaaccgg tgaaaatgta ttctatggcg aatggcttat caatgctcag ggtgaagatg
     1201 tggttgccgg catccgtaca ccaaacccgt tgaacaacga aaccaagaac gaccaaaaca
     1261 agcaccttcc aagcatggag gagctaatgc ccgataccta caaggagctt gtggctgtcc
     1321 gcaacacgct cgaaaagtcg ttcaaggaca tgcaggatat tgaattcacc attcaggaag
     1381 gcaagctata catgctacag tgccgtacgg gaaagcgcac cgggacagcc gccctgaaca
     1441 tggccatgga tatgcttcac gagggcttaa ttgacgaaaa aactgttgtg ctacgcgtgg
     1501 aaccagccca gctcgacgag ctgctgcacc ccatctgcga cccggaagag gagaaaaaag
     1561 ttaaccccat tgtaaaggga ctaccggcag gtcctggagc agctgttggg aaggtggtat
     1621 ttaccgctga agatgctgtt gctgcagcaa gaagaggcga gagtgttatc ctggttcgcg
     1681 aggaaaccaa ccccgaagac gttgaaggta tgagggctgc agttggcatc cttacagcca
     1741 gaggcggaat gacctcacat gctgctctgg ttgcacgcgg ttggggaaaa tgctgcatag
     1801 ttggagcagg cgccatgctg gttaattcat ccgaaaaaat tgcaaagatt tccggtacaa
     1861 acatcgttat taaggagggt gacacgctta cccttaatgg aacccgtgga cacgtatact
     1921 ccaacgcact taagctaatg gaggcaaccg agaacccacg cttccgtgag tttatgaagt
     1981 tggttgacaa gtatcgtaag atgggcgttc gcaccaatgc cgacacacct gaggatgcta
     2041 agattgcccg cgagtttggt gctgagggaa ttgggctttt cagaactgag cacatgttct
     2101 acggtaaagg ttctgaggaa cccttgttcc tgcttaggaa aatgattctt agcacaactg
     2161 ttgaagaacg tagaaaggcg ctggacgagc tctttatatt tgtaaagcgt gatattaaaa
     2221 acacgctagt tgctatggat accctaccgg ttaccttccg ccttcttgac ccaccccttc
     2281 acgaatttgt tccccaaagc ccggaaaaac aggcagaact tgcaaaatcg cttggaatta
     2341 ctgttgaggc tgtagctaag cgtggcgaaa acctgcacga ggttaacccc atgatgggac
     2401 ataggggagt tcgtttggga attacctatc cggaagttac cgaaatgcag gtgcgtgctg
     2461 tttttgaagc tactgctgaa ctactaaagg aaggcaaatc accacatccc gaaattatgg
     2521 ttccagtaac ctgcgaggtt gccgaactcg acttcacccg taagattgtt gaccgtgttt
     2581 acgccgaagt ctgccaaaag tttggaatga aatccatcga attcaagtat ggcaccatga
     2641 tagaaatacc ccgtgcatgc cttctggccg atgagattgc ccgtaccacg gagttcttct
     2701 cttttgggac aaacgactta acccaaatga catttggatt cagccgcgac gatgcgggtg
     2761 gtttccttcc cgattacctg gataaaaaac ttttgcctgc cgatccgttc caaaccattg
     2821 accaaaaagg tgttggccaa cttatacaaa tggcggtaga aaagggtcgc agcactcgcc
     2881 ccgagcttaa ggtgggtatc tgtggcgaac agggaggtga tccggcttcg gttgagttct
     2941 gcttcaaaac cggccttacc tatgttagct gttcaccctt cagggttcct atagctcgtc
     3001 ttgcagcttc gcaggcaagc attaagtatg gaaaatagtt ttactgtttt ttcctaccaa
     3061 cgcctccagt ttggaggcgt tttttttatt tttgtgccat gagcaggcaa aaatcaaaat
     3121 taatccgagt tattatccag ctattcctac ttattctccc tgccacctgg ttgtgggcac
     3181 aggatgatga gctgctgcca acagatacgg cagattttaa atcacactac aacatgctgt
     3241 tgatatttga ggctgactcc gggcataccg acatggttca gtacctgctg gagctgggtg
     3301 ccgaccccaa cacgcaaacc gagcaggggg taacacccct catgtacgcc tcgcaatggg
     3361 ggtataggga cattgccgag ctgctgctga aaaatggcgc caaccccaac cttgcaccat
     3421 ttgatggcaa tacagccctt catgcttcgg ttaaagcggg caacgatagc attgccgaac
     3481 tcctgctcga ctataacgcc gaggtaaatg tagccaacag gaaaggcctt acccctcttc
     3541 actatgctgt ttggtatgga tttccctacc taaccgacct gttaatttac catggcgctg
     3601 atgtaaatgc tcaggacaac catggaaaca ctccgctaat gctagcggtt tacaatggca
     3661 caaacctctg caccacccta ctgctggaat cgggagcaaa ccctgacatt caggataatg
     3721 agggaaaatc accactaatg gtggcagctc agtttaacga caccctttta agcaaattac
     3781 tgcttagcta caacgcagat atcaaactac atgataaaaa aaatgccact gcacttgctc
     3841 ttgccattgc taatcgttcc tcacagacca ttcaacttct ccttgagaat aatgccggta
     3901 gctatacgct ctcaaagagt tactaccagc tagcagccga aacagggagt agagaggttt
     3961 tagagattct taggaaaaac ggattaaaaa ctaagctcaa accctccata ggcagcattg
     4021 gggttggaac gggctttata tttaacaatc atgaatttct ttggggtttt gaaatcgaac
     4081 cagttgaaag cataagcaaa acgggactat cagttggcta ctggtttcgt ccttcacctg
     4141 ttgctgtgct ttacccaaaa aacaacaagc tattccagct ccacgaggaa aagcaggttg
     4201 tttcagtaaa gctgcgacac tacattccaa ttgtatttcg ccctaaaggg atcgggttga
     4261 aagcccagtt tggcccatct ttcgattgga ttagtcgaaa taccagcgga accgttacca
     4321 accctccgga tgtggtaagg ctgggagcaa atgcagccct gaatttgcac ataaactctt
     4381 ttgaaattac cgttggatgg gactacacat ggttaaaaac actggatgcc tcgcctcata
     4441 gagcaaatat taatatcagg tataacctcc ctattgccaa gccaagaatt gtaaacaaaa
     4501 cgataaaata tgtcaggtag attaaacaaa gggttgattt tcatcgttct acttgcagct
     4561 atccccttat catgcgagga gctgaacagc tggtttgttg actgctcgca atgcttcaaa
     4621 gacaaaattt cccgcaccga cattaccgta aagctaacca tcaatgacga aaatccctgg
     4681 gtcatatttg atgtttacag ggggaatacc gacagcggag agctgctatt caccgatacc
     4741 tccgtcgtag ccgaatacta ctggcgtgcc gaaatcaacc agttctattc tgttaaggca
     4801 agatacagtt gcaagggaag ggtaatatac gtagttgatg gggctatgct taagactaag
     4861 ctggaaagta gcgcctgcga tgaaaaatgc tacactatac acggagacgt tctcgacgca
     4921 cggttgaaat attagcccta ccataaaatc attttaaaaa tgatattttt gccatagaaa
     4981 aagcttatca ttatagctgt aataatatag acttatagct gtatgcgtga tcaccctacc
     5041 ctagtaggtt ttcaagggca aacatacatt tcgaatgttt tgctgatgag ctgcaaccta
     5101 aataccgaat ggcaaaatca acgtgctctg atgatgcaac cataaatgga caaattaaca
     5161 aaacaaatgg ataggtttta gagtgtggct acccataaag caacatgcta aagcatttgt
     5221 aataggctac caaataattg aatccttatg aaaagaaacc ttgtaaagct gctgcttata
     5281 gctgtaataa cccgtttcgg tgtatggagt gcccttgccg atgaggggat gtggatggta
     5341 aacttaatcc accagaacat tgcaacaatg caaacaatgg gcttaaaact cacagcagag
     5401 gatatataca gcctgaacca ctccagcctg aaggatgcca ttgttcagtt taatgatgga
     5461 ggttgcacgg gagagctggt ttcggcccag ggaatgttct ttaccaacca ccactgcggg
     5521 gtggaggccg ttcaggcctt aagctccgaa aaaaataacc tgctggaaaa tggctactgg
     5581 gctggcagcc tttccgagga gcttcctgcc catgaccagg ttgcccttat tttggttggg
     5641 gttgaggatg ttacagccaa agtttttgcc tcgcttaatg caggcctctc caaccaggag
     5701 tattttgata aaatgaggga ggttatgcag cagcttgagg atgaaacatc gaaaaaaacc
     5761 ggctaccaca ccattgttaa gccattctac aactacaacc agtttttcat gttcacctac
     5821 gaacggtttc tggatgtcag gttggttggg gttccaccta gctcaattgg taactttggg
     5881 ggtgatgttg ataactggca ttggcccagg catacaggtg atttctgcat attcagaata
     5941 tacactgcac ccgatggcaa gcctgcagcc tacagcccaa gaaacgttcc ctacaaacct
     6001 aaaaacttcc taaaaatcag cctaaagggt gtaaatgaag gcgattttgc catgattatg
     6061 gggtaccccg gcacaaccta ccggtatgcc acttcctacg aagctcagta cagccgcgat
     6121 gtggtagctc cctggaaggt tgatgtttgg ggcaacttca tcagcattgt gaagcaggcc
     6181 agaaattaca accccaaggc caaggtggcc tacaccgaca agcacgacta cctggtcaac
     6241 ttctatcaaa aggatgtttg gcaagcccag tcaatgttcc gatacaatgt agttgaaagg
     6301 cttgcagctc gtgaggatag cctaaaagcc tgggttaacc aaaatccctc gcttcgaagc
     6361 aagtaccttc cgaccattcc tgttacccga aacttttttg aacaggctac ccaaaacaaa
     6421 tgggaagcac ttgaaaactc gctttcggcc ctttcctttt ttcctgtcga tgtttacaag
     6481 aatgtaaatg cctgcaacaa cctgcttggg gccatttttg agcaaaacaa accctacagc
     6541 ccacttaaat tctggaaagt tgaccgcatt agaagggaag ccagggtact caagaaaaat
     6601 attcccggca tttttgacag ctacttctat gagcctgata tgcgcttgta cgcaacagca
     6661 tttggtaacc ttatagccaa tattggcgat tgccaaaatt taggcctact taatgcgata
     6721 aaaaaggttg acaacatcga cagcctatac ccatactaca tacaaggttt ctatgaaaag
     6781 tcatatttta cgaaggctca aaacctggag aagctcatca agtaccccta catggactcg
     6841 ctgcttaccg acccgctttt catcctttac cataactata gtgcgctatg ggattcaatt
     6901 taccctcaga tgcacaacgc cagaatggat tttcagaagg ctatgcagct ttacaccaaa
     6961 gggttgatgg agatgaatgc aggcaagctg ctttacccgg atgcaaactc caccatgcgg
     7021 ttaacatatg gcagggttac cggctacaag cctgctgatg ggctttacta caagcccttc
     7081 accacacttg atggcgtgat ggagaaggaa aatccaaatg aggacgtctt tacggttgac
     7141 ccaaaactac gtcagctttg gcaaaacaag gattatggac gttatgcaag taacggtaca
     7201 atcccggttt gctttctgac cgataacgac ataaccggag gaaactccgg tagcccagta
     7261 ctcgacggta atgggaatct tattggtctg gcgtttgatg gaaactccga agccatggcc
     7321 tgcgacttca tgtttgagcc cgagacccaa cgaaccattg cagttgatat ccggtacgtg
     7381 ctttttatcg ttgataagtt tgccaatgcc cagcacatca tcggcgagct cacaacccta
     7441 taatacaaca gcacacaagt tgttggcctg aggtgtgctt ttaagctgat tgcaaatcaa
     7501 ctgaagaaat tctgcacaac cttcttaaac ctcaacaaca agtccatcgt aggccaggaa
     7561 aacattgtcg ggtagctgcc gggcaacatc ccgatagaat cccatttggt ggcttatatg
     7621 tgtaatgtag gcttttcgcg gactaagctc ctctaccagc tttaaggctt cgggaagaga
     7681 aaaatgcgaa aggctttttt gcatccttaa agcattaatc accagcactc tggagcctac
     7741 aattttatcc ttttcctcct ctgaaatgaa gctgccatcg gtaatgtagg tcaaatcgcc
     7801 tatgcgaaag ccgaatattg gcagcttgca atggtaaacg cgaatgggga ttacagtaac
     7861 tccaagtatg tcaaattgat gcccaaacac ttggtgcagc tccacttcgg gaataccagg
     7921 atattttcgt tcggcaaaaa tataggcgta ctccctctga atggcctcca gcaccctatc
     7981 ttcggcccaa atatccatgg gttgacgctg cacccagttg aaagccctaa catcgtccag
     8041 cccggctaca tgatcgcggt gctcatgcgt tatcaaaatg gcattaagat ttttgacgtt
     8101 ggcgttaagc atttgttgcc ggaaatcggg accggcatca agtaaaatgt taataccttg
     8161 ctcctcaata agcgccgatg ttcgtaaacg cttgtcgtgc gaatcggttg aggcacacac
     8221 cggacatgaa caagctatca cgggaatacc ctgtgcggtt ccagttccta aaaaagtaat
     8281 cttcactgta aaaaaaagtt agaatagctc atattatctc agctggcaag ctaactatcc
     8341 aaccacaaca tcaaccagca ggaaatgttc tatgttactt caatccagct taccaatatc
     8401 ccaacgaaac gctaccttag caaaaatttt cagtaaatat agcttgttgc agtaaataaa
     8461 aactaaagga tggccggaaa actttacctg gtacccacga caattggctc aactagctgg
     8521 aacgaagtga ttccaagcgg agtggtagag ctaaccagag ggttgaaatt ttttgtagtt
     8581 gaagaactcc gcacagctcg tcgttacctt tcagcactag gcattcaaac acccattgaa
     8641 aagctttgtt tcttcactct taacgagcat tcctcagctg cagacttaac cgatatgctt
     8701 aaacccttgc ttgatgggaa tgatgttggg ctgatgtcgg aatcgggctt accagcaata
     8761 gccgatccgg gttcaatgct ggtgtcggca gcacaccatc atggaattag ggttgttcca
     8821 ttgtgcggcc ccagctccat aatcatgacc atgatggcca gcgggctgaa cgggcaaaat
     8881 tttgcctttg ttggctacct ccccgttaaa agtgaggaga gaaagcgacg aataaggcag
     8941 ctggagcaac gttcgagggc ggaaaaccaa acccaacttt ttatcgaggc accctaccgc
     9001 aacgttgccc tcttcaggga tattctccaa acttgtgccg atgaggccag gctaactata
     9061 gctgcaggcc ttaccctgga gggagagtat attgccacaa aaaaaatcag ggagtggaaa
     9121 cagcttccca tccctgatat caacaagatt aatacggttt ttgccctgct attttgactg
     9181 tggtttagtt actacagact tactgctgaa gtatagatcg aaaattaggg gagagtaagg
     9241 cggtatggtg gtactcccta cagaatcata accacagtaa gagggcataa ttaccctacc
     9301 ccattcgccc ttcttcatca gcacaagcgc ctggctaaac cctttaataa cccctgtccc
     9361 cacggttacc ttaagggaat ctgtagagga aaagcttcgt cccaacctgg tagcaacact
     9421 gtcgatgttg ctatcgaaca tgtagccatc aagcaacatg cccttatagt aaacataagc
     9481 aaccgaatcc tttgccagag ttaccgtgtc gttggttgga ggcgatagct gaatgtagta
     9541 aatgctatcg ttaacaggtg ctaggccagg gtagtgtaga ttcatgtaat cgtgaagcag
     9601 ctgaagctcg tactcgcgag gatttttgac taccttatgc agctcaacct cataaacaag
     9661 cgattggtat ggtcttataa cagaactagt gtatgaaacg cttcgtttcc cgtatgcaag
     9721 cttagagggc ataatcagca tggcttttcc gccttccttc atatagccca atccttcgta
     9781 aactcctttc accatttcgc gttggtaacc ctcaaccgta gagttagact tgtcgtattt
     9841 tgtaaatagc tgcgagtagt gaacatttac attatactca ccataaagca cagctgttgc
     9901 gctatcattg gttgacagga ctattccgtc gagtgatttc tccttgtagg agtatataac
     9961 aaaatcaccc tcgctgggcg aaagcccagt tccttcctgc tggttgacga agtacagccc
    10021 gcttggctta agcgattcgg taggaatacc gttgacctgc acccatgcct ccagcatagc
    10081 caattcatct ccctccttat cagaaaaatc cctgtcgcat gcaaccaacg caagtgcaag
    10141 tataaatacc gaaatcaata aaaaaaatat ctttctcata gctaccattt agttcaagat
    10201 tcaaaaacct gtccgcctac cggcggattc acccttcgac tggctcaggg ttcaaaactt
    10261 gtccacctgc cggcggnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnggcg
    10381 gattcaccct tcgactggct cagggttcaa aacttgtcca cctgccggcg gaacttgtcc
    10441 gcctaccggc ggattaaaag tattcatctt cttccctcta acttttaact tttaacatct
    10501 tcacggttca cgtcacacgt atttattccg ccccttcagg aatagaaaat tgcacgattc
    10561 aaaggttaat gattacagtt caatagaata tctactacta ataaaaacgc aaaataaaca
    10621 aattaccctt taaaaaattg cccaaaataa aacatcatgt catttaacaa tgctaaaaaa
    10681 tcataattca gggtttatgc caacgcttat ttaatgctat cgagccgaat atcaacaaca
    10741 ataggaacat agggcatcac cttgctactt ccgcttttac catatcctaa tggagacgga
    10801 gctaaaattg tgattttgcc ccccagcgac gccagctgga aacctagccg tagcgcttcg
    10861 ggcatttggc cagtaaggtc aactttctca ttcgccccgg gtgtttgggt tacaacagtt
    10921 ccatcggtaa gggagatgct gtaccaaccg taaaccacgc tgttcgacag aggtcttact
    10981 gttgaagaat cgagaaggta cctatagtac aagccgctag aatggagggt atatcccgtc
    11041 agatccattg atttaataat gttttgctcc gattctattc ccctaccgtt aagggcaacc
    11101 acctcaatat cgtaggccag gggtgtccac ggtggcagcg ggccaacctg attgtcacca
    11161 aatccgatat cggatgtaaa aaatatggtt gatttttgcc cttgtctgca tagcagcagg
    11221 cctcgtttta gcccctcaat aagtttggtt ttccctgcaa tgactctaac aggttcaagg
    11281 gtacgggtat tggtgtcgag ttttgccaaa acagcctccg acttgatgtt ggtgtcgaaa
    11341 accagcacgg gggactggat ggtgtagccc ttataccaga aggtaaccgt atcgccgtag
    11401 ttaacctcgt agccgtagga tggctgaact acaacatggt aaacaccctc ggacttggtg
    11461 taggtccact ttttagaact tataagaccc tcaataatct tttcctcatc gttgcgctta
    11521 tcctccttaa gcgagttatc gcaggcaccc atcaatagag gagacaaaag tagaatagcg
    11581 ttaaagaaat ttttttgaat catagctgaa cacctttttt cacctcaacc acctccagct
    11641 caaaaaccag agaggtaaat ggcggaataa ttccgctggt gtttccctgc tgaccaaaag
    11701 caagatgcga tgggacaatt agcattgatt tctcgccctc gcgcatggtt cccagcgctt
    11761 cgtcaatacc tggaattacc tgccacttgt caccgtaaac aagctgaaag ggttcgttac
    11821 gcttgcgggt ggagtcgaat attcgcccat caaaaaagcg accttcgtag tgaatggtga
    11881 tggtgtcccc tttttcaacc ggagctcttt ccgttcgtat ctggggaata atgtacaggc
    11941 ctgaaggtgt aggctcaact gatagcttct gtccatcaat atactgcctt atgataacct
    12001 tttcgtaatc gccaaaatct tcaatccacg ataaaaaggc ctccatatcc tttttgaact
    12061 cctcctgggt tttaatgctg tgcaaacgaa tgtcaacccg catgtagtcg ttaacaccaa
    12121 taaatgtggg cagcttggtt tcgagggttt gagtaaaaaa cggcagcgcc tttatgtaaa
    12181 aagcaccgct atcgccctca gccagcatca tgaagcactc gtcaatagaa ccttcgtagc
    12241 ttgactgggc aagctggaac tgccgggttc cttgaaagaa aacggagtcc ttagcagtac
    12301 ggtaggaaat actagcggta atgtaattag agggctttgc cgtagaagtt ccgttgccaa
    12361 gcgaaataag cttgtagtga attccggtgg gggttacaga aaatccagga aaaagtttcg
    12421 accggttgga gcagccaacc gtagccacaa ccagaaatgc taacagaata cctgaacttt
    12481 ttgtcatgca gctaaggaat taatgcgtgt aaagttagcc aaaatatcaa aaatcaacga
    12541 taaacagcca acaattcaac atcgtaaata actattgacc tagcgggtac ttgtttgcta
    12601 tcgccaatca ggccgtaggc caaatggggg ggcatgataa atttagcgct ttgtccctgc
    12661 ctcatcagca gaatgccttc ttctaaacct gaaacaacac ccccatggcc aacctcaaag
    12721 gtggctgggg cttctgcatt ggattggtag caaacggttc catcgagcag cgaaaccctg
    12781 tagctatact caacaacatc gccctttcta accttggggc cattagatgt ccctgaaata
    12841 tagtaaaata aacctgtagg gctttccttc atctccacca agttgttccg cttaatgtag
    12901 tccagtattt gctccctatc ctgctgaacc aacaccttgt tgatacgctg aagcatctcc
    12961 ctagtttcag ccacattggg ctgcctgtcg ggctggcgct catttcctcc acacgatacc
    13021 aggataacgg ctaaagggat gatggttagt attttcatca gcaatctatc tcaacgtctt
    13081 cttcacctat atttatattg ctgcagcatt caactcctgt aaatgctggc ggagaagctg
    13141 ctcgaaatca gccaatgtag cgtcaatagg ctgaaatgat ttccctccgg cagcattaag
    13201 atggccgcct ccgttgtagt acttacgggc aaactcgtta acagaaaaat tacccctcga
    13261 ccgaagcgaa accttaatat ggttatcgtt ctcgacgaat atcaccgaaa aaattatccc
    13321 cttgatggaa agggggtagt tgactaaccc ctcggtatcg cccatttgat agttaaaccg
    13381 cttgagttcc tccctggtca accagatata cgcagcatga aactcaggga aaaccatcat
    13441 tttatctgcc aggcagtaac ccagcagctt cattctatcg gccgaaaagt tattgtaaac
    13501 caacgattga atgcgctcaa caacggctcc actgtcaacc agcattccgg caatttcgag
    13561 ggttctaccc cgagaacagg cgtagctaaa cgatccggta tcggtcatta tgcctgcata
    13621 caggcaggta gctgcatcgg cgttaagtgc tttgagcccg taaatttcag tagccagctc
    13681 gaaaaccagc tcggaggtgg agctcacttc cacaaccgaa atggataaat cgaaggtatt
    13741 ttccggttgg ggatggtggt caataagaat ttttttggct gaagaggccg aaaacaagga
    13801 ttccatatct tctgtccggc taaacccatt gtaatcaaga caaattataa tatcggcacc
    13861 agccatcacc tcccttacct tttgcctact gctggaaaat cgcagaatcg tatcgcttcc
    13921 cggcatccat gaaagaaaat cggggaaatc gttaggggta acggcagtgg catgaacccc
    13981 aacctgctca agtatatgcc atagcccaag cacagagcca acagcatcac catcagggtt
    14041 atgatgggtt gttagcacta ttcggttggt aggaaccagc aactccctta atgcccctgc
    14101 ccatgcctca tcaagattca acttcatcga gcttttattt tactatgatg cacccatgtt
    14161 tttggagcca caagctccat tgtaaaataa ccaacatggg tgtttcacca tacctttaaa
    14221 atcaaatttt attatgatga aatttacaaa gatagatttt gccgacaaga aagttttgcc
    14281 tatttttaaa aaattccgga aaagaggagt gccgcttcac cggaagtatt ttaaacatga
    14341 atttcaaaat ggaagcattt gcccgctaga gctgctgttg tattaatgcc agacatgtta
    14401 aacaggttaa caaaaatctt aaagtatgaa cacccgattc acattttgca tgatcaaacc
    14461 cgatgctttt aagaacgact gcgtaagcga aatactttgt gaaattctta aagccggctt
    14521 taaagtaagg ggcataaaaa ttaccatgct aaccaaggct aaggccgagc aattctacga
    14581 catccaccgg ggtaaggatt tttttgacaa gcttacaacc tacatgagtt caggccctgt
    14641 gatggcaatg gttttagaaa aacctgaggc cgttgaagcg cttcgaaagc tggtgggaaa
    14701 aaccaacccg gaggaagccg acgagggaac aattcgcaga cgatttggtg tgaataccac
    14761 tcaaaatgcc gtacatgcct ctgacagcta cgagaatgcc gagcatgaat ggggattctt
    14821 cttttcgaaa cgggaaatct actagacaaa agaattttct taccctgacc tagcgctcgt
    14881 catctcgtat atttaaaatg atgggaaaaa tagccgtaaa agaaaatcag cattattgag
    14941 cccattttcc actatggaga ctacctataa tgcaggtaga taaagaaatc atcttatagc
    15001 taacaacaac cctagcttac aacaaaattg ctcgccccta ttcctaagat agtttagcag
    15061 gtaacttttc ccacaaatcc gctactatta aaaggttata gaatcataac gacttataat
    15121 gctgatatta agccatttgg tcgtttgtgc aaaggaacat gtagtttgta cttagaaagc
    15181 tgctttatgt cgcaaaataa aattttgcat tattcagcaa cttaaattac gtcccgttaa
    15241 gtaaaccttt tttttaacca acctaaaatt aattctatta agctatgaag aaaactttac
    15301 taagtttctt gggtgtatgc ttaatggcag ctgcaatggt aaccaaccaa gccgttgctg
    15361 tgccggcata ccctggcctg ataaagtacg agcaggccaa cggaaagatc atcaacatct
    15421 atttaaaggg tgatgaaaaa gttaagtggg tgctaagcga ggatggctac acccttatgc
    15481 tcaacagcaa gggagtttac gagtatgctt ttcaggatgg caagggcgat ataacgctat
    15541 cgggcgttga ggcaaataac cctgctgaaa ggacaaaagc ggaacgtgaa tttttaggaa
    15601 aggtaagcaa ggggttgtac tatagcccaa gccagctttc gctgatgaaa agtatttggg
    15661 aaataaaatc ggctgagagc ttgaaggcct tcccaacaac aggcaacaga aagttgattt
    15721 gtatattgat gggattcaag gataaagcct tcaccaaaac acaatcggat ttcaacaacc
    15781 tgttcaacca ggttggctac actacaggcg gtgctactgg gagcgtcaag gacttctaca
    15841 tggaaaattc atggagccag tttaacctta ccgttgatgt atttggcccc tacacctcca
    15901 gccagaacat gtcgtactat ggtgcaaacg atgcctctgg ttatgatgtt aacccaagag
    15961 cattggtaac cgaagccgtg aatgccgccg atgcctcggc caactatgcc aactacgaca
    16021 atgatggcga tggcacggtg gatggagttt acgttatcta cgccgggtat ggcgaggaag
    16081 caggtgccag cgcagattgc atttgggctc atgcctggag cataactccg gttacaaagg
    16141 atggcaaaac catcagcaag tactcctgct cctccgagct taggggaact tcgggaacaa
    16201 caatcactgc aataggtgta atttgccatg agtttggtca tgtgctgggt gctcccgatt
    16261 actatgatac cgactacagc accggtggcc agtatgaagg aacaggctac tgggatttaa
    16321 tggccagcgg atcgtggaac aacaacgggg taacgcccgc ccaccataac gcctacacca
    16381 aggttaaggt ttacggctgg gctactgcta ccgttctctc ctctgcagcc accatatcgg
    16441 ttaccaatgc cgaggctaac aagagctttt accagataaa ctccaccact accaacgagt
    16501 actggattat ggagaaccgc cagcaggttg gttttgatgc caacattccc ggtcatggtc
    16561 ttttaattta ccatgttcac aaggatattg ccacggcaag cagcagcaac aacatcaacg
    16621 caacctatcc acaaaaaatg tatcccgttt gtgccagtgc aaccaccaac cccggcagca
    16681 ctccgagctc atacggtagc attagcagca ccggatgccc atggcctggc tctagcagta
    16741 aaacatcatt caccgatgcc actactccca acatgaaatc gtgggccggc gctaacactg
    16801 ccaaacccat gaccaacatt gccgaaaaca cttcaaccaa aaccgtaacc tttgacttca
    16861 tgggcggttc tggtggcgga acggctccca cagcaaccac gctggcagcc tcggcaattt
    16921 caaccagcag cgcaaccctg aacggacagg tgaatgccaa caacgccact acaacggtaa
    16981 cctttgaatg gggaaccact acaagtctgg gcagcagttt tagcgcaact ccaagtagcg
    17041 taactggaac aactacaact aacgttaccg gaaacctcac aggtttatcg gccaacacta
    17101 cctactactt tagggttaaa gcggtgaatg ccagcggaac aacctatgga agcaccctga
    17161 acttcaccac ttcgagcaca ccaacctcaa tcacacttcc cgttacagaa aactttaact
    17221 catccacact accaacagga tggactaccc agaacaccgg taccagcatt accgagcgct
    17281 ggagtgtttc caacacctac aaggctggtg gctcggccta cgagatgaag tgcacctacc
    17341 agaacgtcaa ccccggcacc accaggctca tcaccccggc catcaacacc gtgggggtta
    17401 gccaggttac ccttaccttt aagcatttcc tggatgccta cagcacaggc gctaccctta
    17461 gggtgcaaac gtcaaacaac aagtcaacat ggaccaacac cacctggtcg gttgccaccg
    17521 gcagctccaa cgttggcccg gcaaccgtaa ccgttaacat tacctccaac ctcaactccg
    17581 aaaccaccta catagctttt gtagttgatg gcaaccttta ccagattgac tactggtacg
    17641 ttgacaacgt ttccattact gcaggtagtg gggcaaccat tcctacggtt acaaccaact
    17701 cggtaacatc catcacctcc agctccgcat cgtgcggtgg aaatgtaacc agcgatggag
    17761 gtgccaccgt taccgaacgc ggaatttgtt atagcacctc ccagaatcca actacttcca
    17821 gcagcaaggt taccagcggc acgggaacag gctcgtttac ggctaacatg acaggacttt
    17881 cggccagcac tacctactac gtgagagctt atgccatcaa tgcccaggga actgcctacg
    17941 gcgcacagca gagcttcacc acgctgagcg gatcaacatc acttcctctt cctgttaccc
    18001 aaaccttcaa cacctcatca ctgccaaccg gatggactac ccagaacacc ggtaccagca
    18061 ttaccgagcg ctggagtgtt tccaacacct acaaggctgg tggctcggcc tacgagatga
    18121 agtgcaccta ccagaacgtc aaccccggca ccaccaggct catcaccccg gccatcaaca
    18181 ccgtgggggt tagccaggtt acccttacct ttaagcattt cctggatgcc tacagcacag
    18241 gcgctaccct tagggtgcaa acgtcaaaca acaagtcaac atggaccaac accacctggt
    18301 cggttgccac cggcagctcc aacgttggcc cggcaaccgt aaccgttaac attacctcca
    18361 acctcaactc cgaaaccacc tacatagctt ttgtagttga tggcaacctt tacaacattg
    18421 actactggta tgttgacaac gtttccattg ttgccagcgg ctcaggttca cccgcattcg
    18481 atactccaat ttcagacctg acagaaaacg atggagtaaa aattttcaac ctttacccca
    18541 acccggcaag cgattacatc aacatcagtc taaccggtgc tagtagcgag gtaacagttc
    18601 gtatcttcga tgtacgcggg tcgatggtta aatcgcaggt tctcaacgca agcgaaaacc
    18661 gaattgacgt aagtaccctg gcaaaaggta tttacatggt gtcggtaggc gaaggtaaag
    18721 agatttctac caaacgcttt attaagcagt agtaggaaac tatccagtca taaaaagttg
    18781 aaaaagccgt ggtagcttac cacggctttt tttaatatca acagcaaaca aagctgcaat
    18841 tgccttagac agaaattgct ggcaagttag aataattcca catgggcata ctccccttgt
    18901 tcccccggtt atgaacatga gcaactttcc tgagaatctg aggttcaagg ttccaataca
    18961 tgaaaattgc tacagctgct aaatttgagg gattttacca tttagtgttg aaaatagcaa
    19021 aaatttaaag ctacggttcg aaagtcaaat ttttggatca ttggtactgg tatagctcag
    19081 caacaactct gcaaaaatcc ctttccattt gaagcaatgt ttcagcgcta ctatagtaaa
    19141 gcgattcctc cagcaggtaa tcaacaagcg aaatttcacc cttttgaagc gcttcgtcca
    19201 gcatcctact gctattaatt gtgcccatgc tattcctgta ttctgctatg tttttttgca
    19261 actccagagc agtattgtat tgcgcttgct gcctactaaa aatttcaacc ttacggtcgg
    19321 cttccacact cctaaggtac tgggcacgtg cctttgcata ctttaccctt ttggaatttt
    19381 cccataaggg tattgaaatt cctgcagcaa taccctgaaa atgctcaccg gcaacctttt
    19441 cactagcgta tcccgcttta atctgaggta aacataacgc cctgcttagc tggatattcc
    19501 tttgtgccac cgcaatctcc cgttcaattg ccttaagatc cggattagac tgctcggtgg
    19561 cagcatacca ctcatcaaaa cttgcaggta ttgcgggcat gttgaaaacg ctatcgctta
    19621 actcaagcgg tatgccacca ttcaggctag ctagtttagc aagtagctcg ttttgcttaa
    19681 gtttaacgct aaacaactct tgtgtggcat taagcagggt aagacgagcc ttgttgttct
    19741 caagtgcgct ggcttctcca acatccaact tttttgaata ggcatcggct attagcttag
    19801 cattgctgac acgcttcccc agcactgatg aaagggcatt ttggtaaacc aactctatac
    19861 ataccagcct aacgtccagc agaagttgtt tactctgacg aaggtactct gttgcaagca
    19921 attctatttc agctttagca atttttgacc ggtaaaggta tgctgtaggg aaatcgaacg
    19981 attgaatcag gctaatgtcc gttttactgc ctatatcagc aggattgccc cactggtagg
    20041 aatactcaaa gctggggttt tgagggtaaa gtcccgttct gctgccaatt ttttcggcct
    20101 ccatcttctg cctgagagca acaagggtgg tattatttcg ctctacttca cccaaaatct
    20161 tctcgatgga tacctgtgcg ttagctattg caacaaacaa caaaaggttg gaaattatca
    20221 ggcttattct tctcattgct tatcagtttt agtcaaaccc cgtttggcca gcaagctgta
    20281 tgcaataggc acaatgtaga tatttaagat ggtggaggtg attaatcctc ctagaataac
    20341 cttggccatt gggctttgaa tttcgttacc agggagatgc cccctcatgg caagaggaat
    20401 taaagccaat gctgccgaca gggcggtcat gagtatagca ttcaacctgt cagttgaacc
    20461 ttttaatatg gtatcgtgga ttggtaagcc ttgggcttgc agctgctgga atttggatac
    20521 cagcagaata ccattacggg tggcaatacc aaaaagtgtt ataaacccaa taatggcggg
    20581 tatgcttaaa attccacctg ttatatagat tgagaaaatt cctccaatga gtgccagtgg
    20641 gaggttgagg aggattatgg cagccaactt aaagtttcga aactcctgaa aaagcaacag
    20701 aaaaataaca agaatggcca gtatggaggt tatcattagt gtccgcgaag cattggcttc
    20761 gctttcaaat tgaccgccat actcaatcct gtatccctcg ggcaaggtga tgtggctatt
    20821 cacccgatct tgaatatcgt tcaccacact tctcatatct cgtcctgcca cgttggccga
    20881 caccaccacc ttgcgctgag cattttcacg actaatggaa cttgggcccg aaactgactg
    20941 aatttgagcc acctccgata aaggcacttt acctccattg taggtatcaa ttagcgcaac
    21001 gccaataccc tcgatacttt ccgtgtagcg gtgattcaac ttcaacacca aatcgaaacg
    21061 tcgctggcct tcgtaaatat ctgccagctt ttcgccacca aaggcaacat ctataaactc
    21121 gttaaagctg ccaacaggaa tgccgtaagc ggcaagcata tcccggttgg ctttcaactg
    21181 aatctgtgga atgtcaacct gctgatcaac attcacatca accagtccct ctatcccgga
    21241 aatctcacct ttaatctggt tgccaatggc aaacatttgg ttaaggtcgg tgccgaaaag
    21301 tttaatggca atgttggcac gagttcccga gagcatgtgg tctattctgt ggcccaaagg
    21361 ctgaccaact gtggctgcaa tacccggaat gccggcaagg gtttggcgaa cgttagcaac
    21421 aaactcatcg cgggagcgct ctgacagttt gaagtttaca tcaatctccg agctgttaac
    21481 cgtttgggag tgctcgtcga gttcacccct acccgttctg cgagccgtac tgtagatttc
    21541 gggaatggca agcagctgct gctcaaccag gttgcctagc ttgttggttt cgtcaagcga
    21601 tgttccgggt ttggttacca ccgacagggt tagcgacccc tcgttaaact ccggtaggaa
    21661 acttcggccc attgaggcaa aagcaactat ggagagaata acaagcgaca gcacagctcc
    21721 caacacaact tttttatggc gaagaaccca ttcgagcgat gccttaaagt aggtttgcag
    21781 attacgtgaa agccacttct cccgttcgtt tttggccagg taactctcgt ttgttagcag
    21841 catctttgat aaaagagggg taagtgtcat ggcaaccacc agggaaacgg taagcgaaac
    21901 aatgaatgaa atgccaagag gtttaagcat tcgcccttcc atacccgaca ggaaaaacaa
    21961 tggcagaaag gcaacgataa tgataagagt agcgtttaga attgaagctc gtatctccct
    22021 ggaagcctca aaaacaacgg taaaggatga ttgtctttca tcaaccggct tgagattgtt
    22081 ttggcgcagg cgtttaaata cgttttcaac atcaattatg gcatcatcaa ccagcgaacc
    22141 aatggcaata gccataccac ccagactcat ggtgttgatg gtaaggccaa aagccttaag
    22201 tgcgacaatt gtaccaagca gcgaaagggg tatggccaga agagaaataa tagttgtgcg
    22261 gaagctgccc aggaaaagga acagcacaat caccacaaaa atagcacctt ccataagggc
    22321 gttctgaaca ttgccaacag aggtctgaat aaaatcggcc tgacggaaaa ttccggtatc
    22381 aagcctaaca tccttaggaa gagttttgta aatgttatcg agatttttct caatgttttc
    22441 agttacctta agggtattgg cattgggctg cttggatatg gaaatgatga ctgcgggttt
    22501 tgtattctcc gaggcataac ccattttggc agcacttcca ataacaacct cagcaacatc
    22561 acttacccta acgggatgcc cattcagaaa cttgataaag gagtttccca acgtctccaa
    22621 gtcggaggca cgagccattc ctctcaccgc atactcgttg ccaaactgac ggatattact
    22681 gccggtagag ttaccgctaa tgtttcggca aactgtggca agctcgtcca tgctaacacc
    22741 gtagctcatc attttaacag gatcggctag cacttggtac tgcttaaaat ctccaccaat
    22801 aatggttacc tgcgacactc cattagtagc aagaataata ggtttaacct cccattcggc
    22861 aatggttcgt aaatccatca tgcttgtgct atcggctgtt aaaccaacaa agaaaatttc
    22921 gcccatcacg ctcgactgtg gggcaagcac cggttgacca acgcctattg gtagctggga
    22981 actaacagcg ataagctttt cgcttacaat ttggcgagct ttgaaaatat cgctgcccca
    23041 gtcaaattca acccaaacaa acgagaatcc ctgggaggaa accgagcgaa cgcggcgaac
    23101 atccgttgcc ccattaattg ccgtttcgat gggaaaagtt accagccgtt ccacctcctc
    23161 cgaggccatg ccatgggcat cagtcatcac cactacggtt ggtgctgtaa gatcgggaaa
    23221 aacatccaca tccatgttgc gggcggtgcg aattcctact accgccagca cgaagaaagc
    23281 aataagtatg gtgtacttat tgctcagcga gaacttaata atcctatcca gcatagcgca
    23341 tcagcttaat gaacatggcc cgcgtgggca tcaagttttc ctgatacctg tgccagcttt
    23401 accaaaaccg ccccgcgggt tacaacacgt tcagtggaaa gaaggccaga cctaatttct
    23461 accctaagcc catcgcttac cccaagttga acctgccgtt tttcaaacgt ttcggggttt
    23521 agctgaacca aaacatagta gttgccctgt tcttcgagaa tggccaatgt gggtacagtc
    23581 agcagcatac tatccgactt ggcaatcagg tttagctcaa caaagcttcc cacaacaagc
    23641 ccgtcggtag catctacctg aataatcacc ggaatcaggt agccattacc agccaaggcc
    23701 gaattcccta ccgacatgat cctgccgttg aacttatcta ttccaacata gctgccattg
    23761 gcctgtttta ccgtagcggt ttttaaaccc tccaaacttt tgaaatattt ttgctgaact
    23821 ccggcattga gcacataaaa tctgctgcca aaaactgtta aaacgggttg accagcctca
    23881 acatactctc cattttgaac ggaaatgtcc tgcacatacc catcaatagg cgaagaaacg
    23941 ttttgtcccg atgcactaaa gttctccttt aggttggtgt atgttgaact tgcgttttga
    24001 tactcgttta gtgcaatgcc cagctctttt tcagaagcca ccttactttt agaaagctcg
    24061 agaattcgtt cgtagttaga cttagccctc tcgaagttgc tttttgcctc ggcataccta
    24121 acgctcaaat cctcattggc caaaccacta cttacaattt ccagtaactt atcaccccgc
    24181 tcgacatgct gccctagcac cagaggttta ctgctataga aagatacaat accaccagtt
    24241 tttgcggaaa taaccatttc gtcggttggt gcaggttcaa cccttgcggt agttttaatt
    24301 acctcgccta tggcttccat gcatggccat tcggttgaaa agtcaacctg ccacgattgt
    24361 tccttagtaa atctaatcgc atcgacaggt atatgttcag catgaaacgc ttccgcctca
    24421 tgttcgttag cgaacaccct aaccaggcct gcgtcaaaga gctcgctttt gccatccctt
    24481 tcaacaatcc acaccagctg tccctgtccg gacagatcgg gtgtaaccgt aaaatggtaa
    24541 ataccatttt tttcaggaat ggcaattgtt tgggagtact ctttaccatt caccgtcagc
    24601 tttatggtaa tagccgcact tgtaagaggt ttaaaatcgg caagattagt aaaatgggac
    24661 aacactgtag tagttgtccc aacaacaaaa ggtttagctt cggcaaatag ctcacaggtt
    24721 gatgtataaa tggtaaaggt ttgagcatca tcaccggcct cagcttcatg actactttcg
    24781 tcatgttgat gggctgcacc agcactgcta tcggcagtgt ggctacagcc ataaattgta
    24841 atgctaaaaa tcagaatgaa taaccgcttc atccgtatat aaaattttaa gttacactgt
    24901 aattacgtat atttttgcat ttcaagcgtt tacgttgaat gcaagaaaac ttaaagattt
    24961 tttttgaaat catcctccat gaactattta cctgcaagca gctaaacgtc gaaacgcagc
    25021 tgattcacaa acagtcactt aaacttgagt aaattacagg aagatccacc atttaggtgg
    25081 ttggaaaaga ttaggctata agaggttttt agccggagga gaccgtaggc caggaatgct
    25141 tacgtagcta gaaaaatgaa aattgttagt ataaccccca tacaaccttg tgtttaccaa
    25201 aaatatttga aaaggaattg aagcacaaga agtaataaag ccaccagcta tgttaaagcc
    25261 atgatgaata ttggtaaccg caggtttata ttcaaaccca tcgtcgggaa tcaggtagtc
    25321 atgctttagc gcacagcatt cagcatcctc gtcggtggga tgagcagggt tgttccttgc
    25381 atcatttcca tcgttcggaa ttgtaatgtt tacggagtat ccatcaaagc aaacctgacc
    25441 gttgtggtga tgatggggta caaaagcatg aacctgtagc attatattag ccagcaggat
    25501 tagccaaacc gaacaaagtt gcttcactcc catcgttggg taaagattaa aaaatcttta
    25561 taatttcgat gaaaaccaaa aggtaaggtt taatttggta agaagttttc tttacccttt
    25621 atccttttat atgttttaag ttcacccgtg gagtttaaaa gtaagctact tccaaggctt
    25681 aagtgctata aatgccagca ttagcataag caccaaagta gcagtaagtc tatttccggc
    25741 tattaaccgc tggttgttta gctgccatgc aagtaaatcg ggatttttaa aaagttgctc
    25801 aggaatatcg cgccaacccc acttcccaat aattgtcccg ttgtaaataa gaattagacc
    25861 ggggttcgat ctggttatgg ttttcagagt aacctcatca cctagtacaa atggaagttc
    25921 aaccctactg tttaccttaa ttttttccat tgcttctccc gaagaggcag tggcccagta
    25981 aaatttatac cccctctcgg ttgcgcccat aaatatattc ttcacttgct cctgagcttt
    26041 tactccagcc ttttcgagct ggggggaaac tgccagaaaa gtataacccg ttgattgaat
    26101 aatcgtatcg gttaggtcaa ctccatcaac cgaatgaagg gagaaattcg aaatgggcgg
    26161 tgtataaccc ttctttacga gtataaattt ggaatcaaca aaagtccacg aagaatcctg
    26221 ccatggatag ttagattcgt caaattcctt tacctcccca tttttcttat agtagagctt
    26281 tgttttatac acatcagcag gggcaccatc gggatatatc attgcctgat gaatgttaac
    26341 acccacatga aatgggcgga agtcgtacaa gggttcatgc agcgaaccat gaattggggg
    26401 aagaaaagcc acaacacaga gaacagcaaa catagcagac tgttttttct cgccaaatga
    26461 aggaggaagt gacgcccggt acctgaagaa agtatatgca gccgacagga ggacaatatt
    26521 tttgaaaaat gtttgccaat tggtcaggtg tagggcatcg ccaaaacagc cgcaatccga
    26581 aaccggatta aaaatagcca ggataagggt aaggggcgta aaggcaagca tgaatataaa
    26641 aacaccccat gcagcaatcc gaagatttac accataaagc aacattaagc caatgagcag
    26701 ctcagctgcg caaagaataa aggcgaaagc tagcgacagg ggctgcataa aattcatgtg
    26761 aaaagccata aagtagtcat gaaacttata ggctgaaccc atgggatcta tgccttttac
    26821 aaatcccgaa aaaataaata ccacggctaa aaggtacctg ctaatgataa cgacctgctt
    26881 cataagtaga aacttttcaa tttaaatgtc aaattactaa tgctttatgt accagaatgg
    26941 cctcaacaat tctgttaaaa attttatccg gctccagcat tgagccatgt tcgtcatagc
    27001 aaggaataac aacaaattgt gggtcaagtg attgctgtag caggtaaacc tctcgaaccc
    27061 gttgctggaa attcaaatcc ttttcgtgaa tatcgtccct cccgttaagg tattccctat
    27121 cagcgccctc cctctgctcg gaaaggcgct tactggtaaa ttcaaatgga acgtctaaaa
    27181 aaatactaat atcgggtttc ggaatggcaa aatagtcaaa ttcaaggttt aaaatccagc
    27241 ttcttagctt atcaacccta ctgctgtcgt taagtttagc gcactgaaag gcaatgttgg
    27301 agtaaacata gcggtcgagc agaaccacct cgtcagtttt gagccacgat ttaattaaac
    27361 ccgacgcatc catccggtca agcgcataaa gcatggctac cacataggga tcaacctcat
    27421 caattctacc caaatcgcct cgaaggaaac gggcaatcag ctctccaaag tatggtgcgt
    27481 caaccctggg aaaatgaagg aaatggctgg caacaccctt ttgggttaaa aaagaaccca
    27541 gcaggtttat ttgagttgac ttaccagcgc catcgagacc ttcaataaca atcagtgcca
    27601 tgagtaaaat gtttaccttt gcagcaaaaa tcaacttgct gtggcaaaat tacatcaatg
    27661 tttagtaact ttagccatcg aacaagccgc aatatagcaa aaaatggaac aaccgccgtc
    27721 gtatctcaag cagaggaaaa ccatgaccag caatggccga attatcagct tggataaacc
    27781 actggttatg ggaataatca atgtaaccga cgattcattc tataatggaa gtcgctacaa
    27841 actggggttt agaatagccg cacgcgccga gcaaattatt cgtgagggtg gaagcattat
    27901 agatgtagga gcatgctcca ctcgcccggg agctaaaccc gttagcgagc agcaggagct
    27961 taaacggcta gcgaaagccc tgcatgcaat ccgaaagaaa tatcccgaag ctataatttc
    28021 ggttgatacc tacagatcga gggttgcccg tatggctgtt ggcgattatc gggcgaacat
    28081 cataaacgac atttcagctg gagagcttga ccctgaaatg ttcagcacca ttgccgaact
    28141 caaaattcca tatgtgctga tgcacatgag gggaaccccc gaaaccatgc agcagaaccc
    28201 tacatacgac aatgttattc gggagattat cacctatctg aacggaaaaa tagaacagct
    28261 aaagcagctt ggggtgagcg atattattgt ggatccgggt tttggttttg gcaaaactgt
    28321 tgaccataac ttttccattc tgaaaaattt acaggtattc aggctttttg agcgacccat
    28381 actcgtaggg ctatcgcgca aatcgatgat tagcaaaaca ttgggaattc cagcagctaa
    28441 atgcctcaat ggaacaacgg tgcttaacac catggcgctt ctgaacggtg caaccatact
    28501 gagggttcac gatgttcggg aggcgaccga ggctattaag ctggtcgaaa gcaccacccg
    28561 tcaacccgat taccagtaca tttaagccaa ctcatgccac aacactctac ttacattatt
    28621 ttgtaaccga gcgccagact ttaaaataat ctatttgcca atgaccaacc ttttcataac
    28681 cattagggca ctcgatgtga tagacgttgt tctggtggca tttttgctct accagatgta
    28741 catgctcata cggggaaccg tagcaataaa cattttcgtt ggcattgtgc tgctctacat
    28801 attctggtta gtagtacgag ctctcaacat ggagcttctg agcagcatac tgggacaaat
    28861 tatcggtgtt ggagtgcttg cactgatcat agtgtttcag caggaaataa gaaagttcct
    28921 cctgtttata ggcaaccggt acattaacag aaggcgattt tcgtttgaaa acttcttctt
    28981 tccaaaaaaa cgcgagaagc aaattcttgt taaggttgat gccatagtca aggcttgtcg
    29041 aaacatgtcg gaaacccgaa ccggtgcgtt aattgtgatt gccagaaaat cggagctgca
    29101 gctttacgcc gagagcggcg atattattga tgccgacaca tcaacccgga tgatcgaaaa
    29161 tattttcttt aagaatagcc cgctgcacga tggggcagtt atcattgtta acgaccggat
    29221 tctagccgca cggtgcgtgc ttcccataac agaaaacatc aacctgccac ctcactacgg
    29281 tatgcgccac aaatcggcaa ttggaataac tgaagttacc gatgccattg ccattgtggt
    29341 ttcggaggaa accggaaaga taagcgtagc ttcagagggg atgctaaggt acgacatcgg
    29401 ctggttggag ctggacaagt accttcgcga taacctatca agctgataat caactgcccc
    29461 atgaattatg atatggtagc ttgcattaat tagcttcatt ttacttacat ttaccgctgg
    29521 gaccatatca taatttaatt cgatgcacca tgattaagta tgccatttcg aaagccgatc
    29581 aaaatttgaa aggtgaaatt accctttctc cctccaaacc catgaaccac aagtttttgg
    29641 ttattcgcat tctcaaaagc tccaacctta cccagcggct aaccaccgaa tcggaggacg
    29701 cacaaatagt aaacagcgag gtaatcaacg agggaattaa aaagaatcgg ggaacctcgg
    29761 caaaagcctt aaggcatatc agggccttcc tcaactactt tggcggcgag tggattctct
    29821 catcatcaaa catccttaag gatagatcaa cagagagaat tgttaaggtt cttcaaaaaa
    29881 gcggtttaaa cgttagctac gagaagcgta gcggcagacc gcctttccgg cttaccggca
    29941 agaacctttc cgggatcatt caacgggttg acggatccat taacagtaaa attattgaga
    30001 gcaagctgct ctttgcccct accgccagca ttgaccaaat tgaagaggtg tgtaatagaa
    30061 ttcttagctc caactacgtt ggtaccaccc ttcgggcact tcagtacctg ggtgttaaca
    30121 ccgattggaa ggaggacgag gtgctggtgg agcatgagat aaacgatggg tcagagatgg
    30181 ttattgaacc cgactggtcg ttagcttcgt gctggtacca aatggttgcc cttgcccaaa
    30241 agggtgagct gcagattaac gggctgaaaa ttgacacatt ccagaacgaa tcggctattc
    30301 ggcatatcta caacgatttt ggcatttcaa ccctaattat gcccgacggc atcagcatta
    30361 agcgaaaggg taagctgacc ggtaagttta cccacgattt taccagctat cccaatcttc
    30421 tcccatgcgc tattacaacc tgtgtaggtc tgggtattcc atttgagatg aagggaattg
    30481 aaagccttaa tgctgcagac cccaacagga tgcagctcct cacggaagag cttgtcaagg
    30541 taggagcaaa agtagagctg aaaagcatta acggaaccca ggtggcaacc tttaacggaa
    30601 cctcaggcct aagccggctt aaaagtgttg agtttgagtc aaaaaacgat taccgcatac
    30661 tacttgctct agtctctatt gcagtaagag gcattcaagt gatcatagac agcccgatgg
    30721 caaccaataa agcttaccct agcttctggg atgatatgaa aaaactaggg tttgtggtaa
    30781 agagtgttac cgaataatag ctattattca gagaacccct tgggctgcca aatcaaccct
    30841 taaaaaaacc gggctttttc gtcataaaag gcaggctcat tgctgtatgc gtcggttaga
    30901 attttttaac agcaggtagg ttggctaccc gctactttgc catgcatgga agccggctaa
    30961 cggccattac accaactcca ccataacttc cggtaagctg caggtatttt tactctaaaa
    31021 attaccattt tgttttatat gcagcgattc cggttattcg tgcgtagcaa gccgtcgttt
    31081 gtcgcaaaat cagcttttgt ttgcgtaatc aacctaactt tcggatgttg aatttcattt
    31141 tcaacaaaaa atcaactcaa accaattaac caaaccaaca aattaaaagt gctatgagaa
    31201 acaaaaaagt gttaaggcta gggttactag gcctacttgc caccatgatg gtagctagct
    31261 gctcaaagga agaagatgaa cgtttgcagg agccaatctc aaacgattgg acagtacgtc
    31321 cggattttgc tactctagat acccgtccac aggatgttat tgccagcttt accctcaccg
    31381 aaaaggatgc agaattggta aaacctgatg aaaatgtagt aaaaggtgcc aagtatgcac
    31441 tggtgattgg aatttcggac tattccggaa cccaaaatga cctgcagtac tgcgacgatg
    31501 atgcaaccga ctggaaaaat aggctagtaa gcgaaggtta ctccgtaacc atactgctcg
    31561 atcggaatgc taccaaaagt gccattgaat cggcagtaaa ctccctggct agcaagtcta
    31621 ttgccggaaa cgaaatcgcc ttttgctact caggacatgg cagtagggga aacatagtta
    31681 ccaccgacct gtactacata agcagctcgt ggtttaagac taagttttcc actgccaaga
    31741 gcaccaaaat gatgttctgc ttcgatgcat gccagatagg tgcaatgaaa acagccctaa
    31801 atgctaccgg cagggttatt gctgttgcct cgagcacatc aacctactca tacgatggcg
    31861 atgcctccat gagaaacgga gtgttcacat actatcaaat gtatggcttt gataacctta
    31921 gctacactta cctcgagccc gactgcagct atgcctgcag ctccatgaag tcatgggctt
    31981 ccgccagaag gcttaaagta gcgccctctt acgctgattc atattcaggc aacttcgacc
    32041 tgtaaactac cacaattcga tacgatgtaa aaagggagtg cttttgcgct cccttttgct
    32101 tatactttag ataagacaga gcggaaatgc cgtaaaggca catggatgtt atacttgcaa
    32161 agtgttaaaa ttcataaaga tatcgaatag cctcgcccaa ctccctgctc caaaatccct
    32221 cggtatgcct gccgttggga acaattttta tcatcaggta ggaatcattg ttaaatccag
    32281 ctgccctaag caagtcggct gctctggtaa cgtcagtaac catcctttca ctctccttct
    32341 cgcctgcgag catgtagaac cgttggtcgc ctttgcgcct gtatttttga atgtactcga
    32401 aaaccttggg tgaaaaccaa agcgagggtg aaaaaacggc ggccttccca aaaacattag
    32461 gatactccag cgctgcgtat agggtgatta gcccgcccat ggagctgcca ataattgctg
    32521 tgctttgcga acgggggtcg gttcggtaat gcttatcaat aaacggtttt aacgtttcaa
    32581 caataaacct aacatactta ttgccatcgc caccggcgcc caacgaatcg tttcgccatg
    32641 gtgagtactc gttaagcctc tcggaatcgt cgttgtaaat tgcaacaaca atggcagaga
    32701 aaccccggta ggtgtatagg ctgtcgagaa tctcatctac atgccactcg ccggcaaacg
    32761 acgtggcatc gtcaaaaaga ttctgcccgt cgtgcatgta aattaccggg aaagggccac
    32821 catcggcata gtttggaggc aggtataacc tgatggtcct attccggtta agctggggca
    32881 tcctcatgct ggttggcagg taccttacgt tttcagaagc agtagatact gccagaccct
    32941 tagccagcat atcgcgccac ccccctacac tcaacttaac agtatcatat gatgtataaa
    33001 gcctgttggg tatatcccgc ccatcggccg aaacctcaac ggttttccag cttccccgac
    33061 aaagcttgta ctcaaatgtg tcaaccactg aaggtaattg tagtatccgt gttccaaggc
    33121 tgtcggggct aaaggcatac attaaatcgt gaggattcca cccattcaac gatgtggcca
    33181 taaaaaccgt atcggtagcg gatgttttag caggtaggga atccagaaga aataccgcct
    33241 gccctcttgc tgctccagca aagctaagca gaagaaataa agagctgtat aatggcaggt
    33301 tgcgtatcaa caacaagatt ttatacaaat ttagtttttt atggtttctg ctaatggaca
    33361 tgctactgct aaaaaacttt tgcagcaacc cgagctatat tttcggccct tcccatggtg
    33421 taaaagtgaa tggcaggggc gcccctggct ttaagttcct tcgattgggc taccgcccac
    33481 tctaccccaa catcaaaaac ctgccggttg tcggcacagc ggtcaacctc ctgaaccagc
    33541 acctgcggaa tgttgacgtt gaaaaccctt ggtaggtttg ttagctgctc cttcgcactg
    33601 ataggtttta tgcccggaat tatagggaca tctatgccag cctcacgaca acgctcaaca
    33661 aatttaaaat atactgcgtt atcaaaaaac atctgggtta ccacatagct ggcacccgca
    33721 tccacctttt ccttaaggta gcgaatatca acatcaatat taggtgcttc ggggtgcttc
    33781 tcgggataac cgcctactcc aatgcagaaa tcggactcct tggggttttc aatcaacttt
    33841 tccaggtaga tgcccctatt gaactcctgc acctgccgaa ccagctcaac ggcatgctgg
    33901 tggcctcctc gcttaggttc aaacctgccg gaaattttgt cggcatcgcc ccttagcacc
    33961 aggatattct gaatacccag gaagtccaga tcaatcaagg catcttcaat atcgtgagca
    34021 tcaaaaccgc cacatataag atggggaacc acatcaatgc cataacgctg gtgaatggct
    34081 gcagcaaggc taatggtacc tggtcttcta cgtaccttaa ccggaacagg ctctcctgca
    34141 gtgtttggca tgtacaccac atcctctcga tgatgggtaa tattgatgta ggcaggcgag
    34201 aactccttca gcctgtcaat tgtgtcaaaa acggtatcta tggtatgacc ctttaaggga
    34261 ggcagcagct caaaggagaa taaggtgcta cgcgattttg ttagaatgtc tattaccttc
    34321 atattgatgg attgttgaag tgtgacggga aaatatcaac ccggatttca acattctgca
    34381 ctaaagttaa aaaaataaaa ctctatcttg caggaatatc tataccgttt taaaaaattc
    34441 actgttcgac gtttgttggg aaaaacctta cagcttcctc aaggcttacg cctaacctac
    34501 gggcataatc gccaagctgg tcattggtaa ccctgccaat attgaagtag ctgcttttag
    34561 gatgcgagaa gtagtaaccg caaaccgacg caaggggttg cataaccagg gtttcggtta
    34621 gctcaactcc aagtaaatcg gatgctttaa gaagatcaaa aataaacttt ttcccccggt
    34681 gatcggggca ggcaggataa ccaggagccg gacgaatacc atcataccta ccagctaaaa
    34741 cttcggcagg tgagaggttt tcgttaggtc tataacccca aagctccttt cgtacctgct
    34801 gatgtagctt ttcggcaaaa gcttcggcca gcctgtcggc caacagttta accatgatag
    34861 ctttgtaatc gtcgcctgca gcacggtact tgttggccag ctcatctacc ccaattcctg
    34921 aagttaccac aaagcaacct agccagtcgg gtatgcccga gtttagcggt gccagatagt
    34981 cagccagtga aaggttgaac tccccatttg tctttagctg ctgatttcta agctgtggca
    35041 gggtagccat caccgaactt cgggtttcgt cggtaaatac caaaatatcg tcgttctgcg
    35101 aggcagcagg gaaaagccct gctacacctg acgctcgaat tgattttgct gcggctatgt
    35161 catccagcat tcggttagcc tcatcgagaa gctttttagc ctcggaacct gtaacaggat
    35221 cggtaagaat ttcgggaaat cgtcccttga gaccccaggc gtgaaaaaag aatgtccagc
    35281 taatgtaggg tcttaactcc gaaatatcaa catttaggaa tggttggatt cccctatggt
    35341 tgggttgtgg cggtgtgtag ctgctccaat cgaacttaaa cctgttttga cgggcatcgt
    35401 caatagaaat cagtgctttt ttctgctcgc tataagtgct tcggagtccg gcgtaacgtt
    35461 ccttaacttt ttgagtgtac atctcatgtt gattcggaga taacaactca gcaaccacgc
    35521 caccggctag cgaagcgtcc tttacgtgaa taaccgaacc tgaatactcg gtatcaattt
    35581 tcagggcagt gtgtagttcg gatgttgttg ccccaccaac aagtaagggg atagttaatc
    35641 cactatgctc cagctctctg gccacatttg ccatttcctc aagtgatgga gttataagcc
    35701 cgctaagccc caaaatatcg gctttatggc caattacccc attaataatt ttatcagtcg
    35761 gaaccatcac gcccaggtca accacctcat accccatgca ggccagcacc acactcacaa
    35821 tatttttacc aatatcatgg acatcaccct taacggtggc aagaacgatt gttccctttg
    35881 acttttgaat tttgctgctt tcatccgaac tacttatata tggttcgaga ataccgacag
    35941 ctttcttcat aacccttgcg ctcttcacca cctgcggaag aaacattcta cctgagccaa
    36001 aaagggtgcc aacttccttc atgccatcca ttaaaggccc ttcaataacg gatattgcag
    36061 accccattat gctaaaggcc tcaagcgtat caacttcaac gtaatcctca tttcccttta
    36121 taatggcgtg ctcaaggcgt ttaccgggtg ggtaactcct ccattccggc aaagctttat
    36181 catctgtctt atcatccttt acattatggg cataggctag tagccgttcg gttgcatcat
    36241 ggcgacggtt aagcaccaaa tcctccgtca acctaagcaa atctggttca atatcggtgt
    36301 aaatgcggag catgccaggg ttcacaattc ccatgttcat accggcattt acagcatggt
    36361 aaaggaaaac cgtatgtatc gcctcccgaa tctggttgtt tccacgaaat gcaaacgaaa
    36421 ggttgctcac acccccgcta actcctgcat agggtaggtt ttgcttaatc catttggtgg
    36481 tttcaataaa gctgaccgcc tgcgatgagt gctcggccat tccggttcca atagccagaa
    36541 tgttggggtc aataattata tcctcggggg caaagcccac tgtttgaacc agaaggttgt
    36601 aggagcgcat ggcaaccctg cagcgatgtt cggtggttgt tgcctgccct tcctcatcaa
    36661 aaagcatgac aacaactgca gcaccatacc gcctgataag cttagccctc cggatgaact
    36721 cctgctcacc ctccttcagg cttattgaat tcacaatgct tttgccctgt gtgcacttga
    36781 ggcccgcttc aataacctcg aagcgggatg aatcaatcat aaccggaact cttgcaatat
    36841 ccggttcgga tgcaaccagg ttaagaaact cggtaattgc aacaggggca ttaatcaggg
    36901 cgccatccat acacacgtcg atggcctgcg ccccaccctc aacctgttca cgagcaatgg
    36961 ctaacgcttc accgtacttc tcctcgataa ttagcttctc gaatttttta gagccggcta
    37021 cgttagtgcg ttcaccaata tttataaaat tactttcggg tcgaatttcg agcatttcca
    37081 gtccgcttaa gcgggtaaag cgcggtaatg taggtgtttt tcgaggtggg tatttggaag
    37141 caacggcggc aacccgctcg atgtgttttg gcgttgtacc acagcatccg cccacaatgt
    37201 tcaccgctcc tgcctgtacc atttgctcca cccaaccggc catcatctcg gcagattggt
    37261 cgtaacctcc aagctggttt ggaagcccgg cgttagggtg aacgctaact ctaaatggcg
    37321 ttaccacagc aagctgcttc acatagggta gcagctgctc ggcaccaaaa gcacagttaa
    37381 gcccgatgct caaaagcgga tagtgtgaaa ccgaggtaac aaatgcttcc agggtttggc
    37441 cggttagcaa acgtccgctc aaatcggcta ttgttgctga aatcatcacc gggatgtcgg
    37501 tttgcagttc ttccatgaca ctggcagcgg caaacagtgc tgccttggcg ttaagcgtat
    37561 cgaaaaccgt ttcaactagc agcaaatcca ctccaccttt tatcaacgct ttgatttgca
    37621 agctgtatgc ctgaaccaaa gtatcaaatg taaccgcacg gtaaccaggc cggttaacat
    37681 cgggcgacat ggatgctgtt cggttagtag ggccaattga gcccgctaca aacctgggtt
    37741 tatcaggact aagtgttgta aaatcgtcgg ctagtcgtcg tgcaataccg gctgcctcca
    37801 aatttatctg ctcaatccaa tcctccaatg cgtaatcggc ttgcgaaact gcgtttgcgt
    37861 tgaaagtgtt tgtttctatg atatcagcac ctgcttcaag atactgctta tgaagcgatg
    37921 caataacatc gggacgagaa agcgcaagca catcattatt acccttcagg ttgacaggat
    37981 ggttggcaaa cactgcgcca cggtaatcct cctccgtaag gccaagctgc tgaatcatgg
    38041 ttcccatggc cccgtcaagc accagtatcc ttttgttaag ctgctcagct atcaattcct
    38101 tcgatttcat taattgcctt tcctttacgt ttgtggggtt ttaagagaga attccaccgc
    38161 tgtattcacc ggtaaagata gtaaacagat tccgcttggg gagtaattct ttaattttca
    38221 gaagtgacga tagcttaacc ctggccacca cccagcggta ttcgggtccg ttataccgtt
    38281 cctgcacctc cagaatatcg aatacgttga ggtccgactc ccggctatac cgaatgtaaa
    38341 ggctaagaac tacatcggta gtatagctgg gaggagatga aaagtaaaac tttttgtact
    38401 cataacggta accgtgcagc ctggcagtaa tgtcgctcag gacagccgag gctgttggga
    38461 aacctccggc tcctttgcca aacatgaact gcttatcgta gtattggccc tcaatgacaa
    38521 ccccgttaaa ctcattttca acattgaaaa tgtagtcgtc gggagcaacc agagtaggga
    38581 ttacaaaaag cgtgaagcag ctttcggaaa attttcgcaa atgggcaacc aa
//
LOCUS       DAOSHM010000021        38325 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_832_length_38325_cov_21.715966, whole genome shotgun sequence.
ACCESSION   DAOSHM010000021 DAOSHM010000000
VERSION     DAOSHM010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 38325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38325
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_832_length_38325_cov_21.715966"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(64..549)
                     /locus_tag="PK839_05020"
     CDS             complement(64..549)
                     /locus_tag="PK839_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72134.1"
                     /translation="MAVAGFKTLHLLIPYIMDNKNFEDNLFFYWTKNLVGTNSRFILN
                     LAIAILFGTLYSFKIAQTNVILLIFGVVSPVIFTLCLYNLILLVSGDKQEVLNFPSVF
                     LVKKSNRLLSIFDSSLVVLLGWLIYRGTLNYFFFRFLLTFFIPVLLIIFLRGLYFFIT
                     G"
     gene            642..2378
                     /locus_tag="PK839_05025"
     CDS             642..2378
                     /locus_tag="PK839_05025"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOG72135.1"
                     /translation="MLKKILLITIFAVALLSLFPNCAKVVTPTGGPKDTIPPALVNSI
                     PKPNAINFRGEKLVLEFDEFLALKDVQQKLLVSPPLNNRPQIKPKGKRIELEIKDTLL
                     ENTTYTVYFGDAIRDNNENNAIPNFEFAFSTGPTIDTASLAGTIVNSFTNEPVEGALI
                     MLYSSFADSLPYIKRPTHMAKSGKNGRFKVNNLKNIDYKLVAITDANGNYLYNQGIED
                     IAFVEGKVTHDMLMNASDSSRWVRLRTFSEPLPYQLLTGTNRPQRDLIELNFSRKPVG
                     GFKLVQVDNPVSEKWYIPEPDNEGDTVKLWIISEKISAADTLKLALSYLKTDSLNMLQ
                     PRIDTLRFVYYESEEAGNDSRSKRGKKEEVVKPTGFKVETSIKNMAVAIPGVPFEFTL
                     PSPARKVNGALVQIFNQTDSILEPGVKLVADSINPRIYRFNTSWKPNVTYKMIVLPGA
                     FESYTSTLNDTLKLQLTGADPESFGTLTVKFSGVSKAVVAELLTEKDAVVDRKAVVGN
                     QSVTFTFIAPAKYKLRFIDDLNANGTWDSGNYLKRIQPERVIPFTEGKNKGVINIRAN
                     WDNEISVSLQKP"
     gene            2386..2685
                     /locus_tag="PK839_05030"
     CDS             2386..2685
                     /locus_tag="PK839_05030"
                     /inference="COORDINATES: protein motif:HMM:NF014022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-binding protein"
                     /protein_id="HOG72136.1"
                     /translation="MSVLMNFAMFPTDKGASVSPYVARVVEGVRSLNHASQLTAMGTI
                     VETSSLEEALQVVEKAYQLLEPDCDRVYVTVSLDIRKGGSGRISLKVKSVEDKLA"
     gene            2816..3526
                     /locus_tag="PK839_05035"
     CDS             2816..3526
                     /locus_tag="PK839_05035"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HOG72137.1"
                     /translation="MNKADIANYFKGRVIIFLFFILLVSEGIKAQPYVGLRAAYTTST
                     MTFKPDAETKLLTGSGLDYGLEFKYFNAKYVGFQSELYLTSRGYLKAIDVEELGDTLF
                     KRVNLYLELPMFIQFRLKLGIVMLHVNAGPYFAYLLSAKEGDNSTGKYRLNKVDINIL
                     RENRFDYGLMGGVGLSHDFQWGTIQAEARFGYGLGDLYDYSYSGMPSESRAIIQNFSL
                     SYLYRFGQHNTQQEVKQP"
     gene            3780..4445
                     /locus_tag="PK839_05040"
     CDS             3780..4445
                     /locus_tag="PK839_05040"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72138.1"
                     /translation="MKAWKEGDYRLQQVISNTRLAPGAFLLKFRKQFDFSAGQIVALG
                     VSTDIEPRFYSIASSEEDELISVLYTEKPDGKLTPMLSMLNPGNPILVSKPFGAFTSF
                     KSEPVFVAAGTGVAPFISALLSNKVRGATLIHGAPYPEYFYFSDYLSEKLGRNYIQCC
                     SRCMPGSHYVGRVTSYLLQWDELDPNRNYYLCGSTEMVVETRSLLISRGVPPDRINAE
                     IYF"
     gene            4534..5568
                     /gene="rlmN"
                     /locus_tag="PK839_05045"
     CDS             4534..5568
                     /gene="rlmN"
                     /locus_tag="PK839_05045"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HOG72139.1"
                     /translation="MPELLPLYGKTLAELQDIVSQEGLPPYAAMQLADWLYKKNVESI
                     EAMTNISKSAREQLVGRYYVGLSKPEGVSASADGTKKYLFRSGNGKFVEAAYIPETGR
                     ATLCVSSQAGCKMGCLFCMTGRQGFQGNLSSGEIINQLRSIPEFKNVTNIVYMGMGEP
                     FDNLENVLRSTEVLTSEWGMGWSPKRITVSSIGIIPAMNTFLSRSSCHLAISLHSPFD
                     EERRMLMPIQNVYPIADVVEEIRKANIAGQRRVSFEYIMFDGLNDSPRHVREISRLLH
                     NLRCRVNLIRFHPIPESSLRSASDETIIKFRDALTSKGIFTTIRASRGLDIQAACGLL
                     STKGLLKNKD"
     gene            complement(5624..6760)
                     /locus_tag="PK839_05050"
     CDS             complement(5624..6760)
                     /locus_tag="PK839_05050"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOG72140.1"
                     /translation="MKQINAEFGVDTANSLLKLINSHNAIDLTAGWTRFPIPVQVADL
                     VARELRQTHGRAPIEGTLELRKHVSEYIQKKYFRTYHPAAEIAITSGAAQAIFSAVAA
                     LVSDGDEVIIFEPTRNDTALAIEVCGARPVYLKLHEDNASIDWHEVQKSVTPRTKLII
                     ISSPNSLTGSILSPDDMENLQKLINGTKLKIISDETLSDIVYPGVIPSSVAFFPKLAD
                     NAYIIGSLSQTLGISDWDLGYCLAPAAMMNNFQRIQRAIANTANHALQLAIADFMSTN
                     ETSFPYHAVIQKNRQILLDSLSSTRFKPLPMQAGYTQTLDYSDVSDLKDVEFAEMLVR
                     DYGVGVSPVSIYFHDKTIRKHLLINLAVEVDVMTEAALRLSKTA"
     gene            complement(6860..7588)
                     /gene="recO"
                     /locus_tag="PK839_05055"
     CDS             complement(6860..7588)
                     /gene="recO"
                     /locus_tag="PK839_05055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HOG72141.1"
                     /translation="MLQKSRGIVLHAVKFRDNSLIATCYSKEFGRTTIQVNHAYGSGK
                     TAGLAVYFQPLDILDLVFYKKETSEISLLKEVAIAIPLSELHFDPVKISISMFVSEVI
                     YRTVREVEPNSELYEFLENSIRLLDVMHDGVANFHLIFLIQLTRYLGFYPANGWSETM
                     PFFDYKNGFFCPSPPSHSFNLDKTSSRRLGMLLGTPFYRSNEISLNHAQRIQAINDLL
                     CYYNFHLGSPLDFKTLPILIQLFQ"
     gene            complement(7592..9883)
                     /locus_tag="PK839_05060"
     CDS             complement(7592..9883)
                     /locus_tag="PK839_05060"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HOG72142.1"
                     /translation="MARVTSAVFLFLFLLQGVLQAQIPVGSWRDHFSFGNIKLLCYEE
                     GKIYCGADNGIFSYNIATGEVDKVTRVNGLSEVGISALSYFPSLQILAIGYESGNIDL
                     LVQNSVVNLPFIKEKPLSGSKTINRFTLFNNLIYTSTDFGLVVVDPAKREIKETYYIG
                     NNGTGIRVSSMEVFGDRFWAATSNGLKSALCSDPLLVSFEHWTTETSFTNPTAECAGV
                     AAKDGYLFALERQENANDILWRFDGSSWVEVDRPLPATLHLSVGNNRILVTSANGIQT
                     YQFPGIRDEFLSPASLFINPQAAISVDADKLAIADKFNGLMFGSSQALRSIYPNGPAN
                     NNHLAIDVSSNRVVVASGGYDATFTNLWHPFMVHDFTHEEWSSFADYSYHDAVAVRVN
                     PGNPSDYYVASWGGGLYQFHDGSMVAHFTPENSSLQSIFPNAPYCRISGLDFDPKGNL
                     WVANTMVANPISVRKTDGTWISFPYASAINSDKLSTLSYSNYGTLWLLLPRGQGLFAL
                     NPGNNIDIKDDDRYRKLQPASSSGSLFNVDINALAFDRDDYLWLGTSQGVLVCFNPGK
                     VFDGGMYFQKIKIPDVVEGLAVYLLETEEVTSVTVDGGNRKWFGTARSGVFLFSPDGT
                     KELAHYTIQNSPLPSNRITDVKVHPASGEVFIATDKGLVSFRSNANQPGSGFGKVYAF
                     PNPVRPEYEGDITITGLMENTLVKITDISGNLVYETFSVGGMATWNGKNSKGQRASSG
                     VYLVFCSASQGEKTAVTKILFIR"
     gene            complement(9933..10514)
                     /locus_tag="PK839_05065"
     CDS             complement(9933..10514)
                     /locus_tag="PK839_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP diphosphatase"
                     /protein_id="HOG72143.1"
                     /translation="MPQKLVFATNNVYKLKEVQAILGNAFQLLSLHDIGFIAEIPEDY
                     ETLEENAMQKARYVFSRTGYSCFADDTGLEVEALNGEPGVYSARYAGEDKKSTENIKK
                     LLMKLNGVENRNARFRTVIALTLSGKDYLFEGTVNGKIIDSPNGFDGFGYDPVFIPDG
                     YNQTFAQMPLALKNSISHRGMAISKLVDFLKKQ"
     gene            complement(10526..11323)
                     /locus_tag="PK839_05070"
     CDS             complement(10526..11323)
                     /locus_tag="PK839_05070"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOG72144.1"
                     /translation="MKGESKTILVPTDFSQVAEYALQHAVRVSGVVKEPITILHIVKA
                     EADKAEAEARLEKIVRGTKEKYGVDITPVVRVGNIFTDIALVADEVQASFVVMGSSTI
                     KEMDENKVSWVLKVITSCKVPFITIQEPPVNKRYDEVVFPVDFTLENQEKHKWISYFS
                     DFYLSRFHLVKPNTTDPELLAKIDINMASARRFLDQKGAKSVEYTVPGLKPYEQEILS
                     LAANIRADLIVLMTTPLDSEGRFTVDPMEQYILANAGHIPVMCINPR"
     gene            11770..13113
                     /locus_tag="PK839_05075"
     CDS             11770..13113
                     /locus_tag="PK839_05075"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOG72145.1"
                     /translation="METLLFQRTNELLKEREKSENLLARVLPQETADELKQFGKIKAQ
                     KFQMVTVLFSDIQGFTKITDDTDSEELIDQLDRFVYEFDSVVQKYHIEKIKTVGDAYM
                     CAGGLPKKNRTNPIEVVVVALEMMSWMKETNRRHQKGDVWELRMGIDTGPVIAGVVGQ
                     SKISYDIWGATVNTASRMESAGEAGKINISENTYLLVRDFFICTYRGQIPVKNRGNIQ
                     MYFVEGILPNLSQNLEGTLPNQDFFIELQLIRLSDLEELVLEKLEQGLPKNLYYHNVK
                     HTVDVYTQVELIGRAEGVSKEDLLLLRTAALFHDAGHLVDYATHEQMGVKLAREILPN
                     YRYSSDQIDEICKLIMSTKLPPNPKNLLEEIICDADLDYLGRTDFIPVSNMLYKEMHE
                     NGMIGSLHEWNILQMNFIQNHQYFTQTARRLRNVNKDMQLQNIKSWINQMEKSNL"
     gene            13246..14082
                     /locus_tag="PK839_05080"
     CDS             13246..14082
                     /locus_tag="PK839_05080"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOG72146.1"
                     /translation="MDSIRKVVLVPWDFTQSSEYALQHAIELAKAGDNEIMLLHIIPH
                     SPLFDIFYKPNFKKIEAINKQFEKVAQDVQSRFGIIPQTLVMEGSPRKIIRGLILTAN
                     VNLIVAEHGYTIKDKTYRTSDFIKNLTFKDITLPFIIVSKPPRHDHYIEIVIPVDYDR
                     KFKEAVHWIIYLAKYYKCNINLIKPFLTDDGQKKQLANNIYFTKKMLDGVGIIYGIKT
                     AKKKSFFKDEVFRFANTIEADLILVMADKYNYFVDAKREANLDIPVMCVNPRIRKYQS
                     FY"
     gene            14103..16922
                     /locus_tag="PK839_05085"
     CDS             14103..16922
                     /locus_tag="PK839_05085"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOG72147.1"
                     /translation="MMKSKPNARKLNLQFFSSLSLKSKILILFTGSTILILIAALIFI
                     SNQLKRQSVENAIAQIGITADQQALMIQNALEKNLYIARTLSGTAKSCSQLPPDRLNS
                     YLTSAYRDVLQSEPNILSVWDSWELKFLDPAFSNSYGRIRNMAWRSNGTILTKSETIL
                     TPDSPQYARIKKELTESVEEPYFFSFDNGSPVLMTSLVVPNIIDGKFAGVSGVDIPLT
                     GFNELIRGIDRYEGSYAFVLSTTLKFIAHPDKNLAGDDAMASYEELFTLHGVPEIIAN
                     GNSAFFEGADASGANSFVAIRPIIIGSCPQSWALVLLVPKGVVLHDATVIFWKTLLVG
                     LAGILLLTLLIQAVARKYILEPIIHVSENLSRLAKGHVDGSMKVHVEVSDELGAMSEY
                     LNRTVDGLTKKTEFAQTIGQGNYGAKMELMSDEDALGQSLIDLSANLKKAQEEVQKRA
                     ADDQKRQWTNEGLAKFSEILRQNNDRLDLLSINILRNLIDYLGANQGGLFVYNDDDPS
                     NPFFDLLAAYAYNRQKFIKKRILPGEGLIGTCAIEKQTIYLTEIPENYITISSGLGEA
                     KPRNLLIVPMKVDENVLGVIEIASFSAIMDYQVEFVEKLAQSIAQTLTTVKTNLKTAE
                     LLEKTQQQTEEMKAQEEEVRQNLEELATIKEELEKRNEDILENQKQLEWEKSLLDSLL
                     NYVPDKIYFKDLKSRFIKVSRSTLKAHGFENPDDLIGKTDFDLFGEEHARPAYEDEQN
                     IIRTDSPIIGLVEKEVFTDGRVSWAETSKLPLKNPRGETIGTFGITRDITASKLMEEE
                     VKKKEMDALAYQRDMQKKALEAQNLYEAINNSTYVIEYNPDGYITFINDAYLELLNSK
                     REDVVGKHHSYQLDFTDEQRKNYKQFWDDLNSGIARKETHRFTVGKRTYLFFETYTPL
                     KDENGKVFKIMKIAVNVSHLLHEGE"
     gene            17159..18481
                     /gene="dgt"
                     /locus_tag="PK839_05090"
     CDS             17159..18481
                     /gene="dgt"
                     /locus_tag="PK839_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007216926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dNTP triphosphohydrolase"
                     /protein_id="HOG72148.1"
                     /translation="MEWTKLLDASRFGATGATKNTYDTARTNYQHDFDRLIFSSPFRR
                     LQNKTQVFPLPGSIFVHNRLTHSLEVSSVGRSLGQNVYLQISETLSPHEKPLVAEIPG
                     IIAAACLAHDMGNPPFGHSGEEAIRKFFEDNSGVFKPMVDSWEWSDLVRFEGNANALR
                     LLTQQLTGRRTGGFRLSNAVLASVVKYPYPSASGKKKFGFFKSEANPFLSIAHATGMH
                     SVGNMEFCRHPLVYLVEAADDICYQIMDIEDAHKLGILSTPMVVDLYRAFFTDEPKGA
                     RDRITSTLKEVQDPNEQVAYLRAIVISKLVEECARIFVNNLKPIMDGEDFSSLLEKIS
                     GASHTAMEAIKNLAVERVYNHRKVVEIEIAGYQILGSLLEIFCDAVLHPQTGLSKKVI
                     RLVPNQYMTSSGDDYTKIMSVLDFVSGMTDVYALETYRIIKGINLPTL"
     gene            complement(18485..19453)
                     /locus_tag="PK839_05095"
     CDS             complement(18485..19453)
                     /locus_tag="PK839_05095"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOG72149.1"
                     /translation="MLEAIKQPVDEELKKFEPFFRKNMGSNIRFLDLITNYIYRNKGK
                     QLRPIIVFLSAGLNGKICQSTYVAAGMIELLHTATLIHDDVVDESFERRGAFSINALW
                     RSKVAVLVGDFLLSKGLLVAVQNKEYELLEVMSNAVREMSEGELLQIEHSRKMDINEE
                     AYYDIIRKKTAYLIASCCENGAISAGADSDSIRRMHEFGINLGMAFQIRDDLFDYQPK
                     GLVGKPNGNDIKEKKLTLPLIYALREAPAAEGRKVLREIRSRSKSSVTISTVVKFTNE
                     YGGISYATGQMLNFKQQAQEVLNSFPDSDYRQSLMLLTDYVTDRNK"
     gene            19987..20295
                     /locus_tag="PK839_05100"
     CDS             19987..20295
                     /locus_tag="PK839_05100"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOG72150.1"
                     /translation="MVKINELKVEQLEDAANMLKAMAHPMRIAIISLLEANGRLTVTQ
                     IHEKLQIEQSTASHHLGILKDKGLLACKREGKNNLYFLRNENLGKILDCVGSCTSTRF
                     "
     gene            20313..20843
                     /locus_tag="PK839_05105"
     CDS             20313..20843
                     /locus_tag="PK839_05105"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HOG72151.1"
                     /translation="MAVFDIVVVILLVVGAIRGLRQGFLSQLASVVALLLGIWGAVRF
                     SDYMATLLSEKFDIGSAHLPIISFAVTFILIVIAIHFIEKIVERFLNLAMLGGINKVL
                     GFVFGFAKYAIITSVLLVFVEKANIRFNFFTEEQKSKSLVYNPVASLAPAIFPYLHFD
                     TIKESVKKSLGNQKGK"
     gene            20958..22250
                     /gene="tyrS"
                     /locus_tag="PK839_05110"
     CDS             20958..22250
                     /gene="tyrS"
                     /locus_tag="PK839_05110"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010991893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HOG72152.1"
                     /translation="MNFIDELQWRGLVHDVMPGTADQLNKEMTSGYIGFDPTAESLHV
                     GSFAQIMLLKRFQLCGHKPIVLVGGATGMIGDPSGKSQERNLLDSATLQKNLEGIRNQ
                     LSRFLDFDSGNPNAAVMVNNYDWMKNFSFLDFIRDVGKHITVNYMMAKDSVKKRFGEE
                     TKEGMSFTEFSYQLVQAYDYLWLYKNLGCRLQMGGSDQWGNITTGVELIRRMTGGEAF
                     ALTIPLITKADGSKFGKTESGNVWLDSKLTSPYKFYQFWLNTSDDDAEKYIKVFTLLD
                     QSTVDALVEEHRREPHLRKLQKMLAREITIMVHSEEDYRKAVEASELLFGNATTETLM
                     SMDEDTFLSVFEGVPLFEVSRQHIAEGVPLSTLAVELAPIFSSKGELRRLVTGGGLSL
                     NKVRVENTELPVSNSHLLNGKYILIQKGKKSYSIIRTV"
     gene            complement(22428..22667)
                     /locus_tag="PK839_05115"
     CDS             complement(22428..22667)
                     /locus_tag="PK839_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72153.1"
                     /translation="MKNLNFTRGQVTNILEGIAKKEEGVQHILKMSIEAIKMVWRDVY
                     NKRHQDISNSMKMQILPLFLHLALGNTTYGREGAI"
     gene            22805..23671
                     /locus_tag="PK839_05120"
     CDS             22805..23671
                     /locus_tag="PK839_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72154.1"
                     /translation="MEQDYRTAEPSFVATVKVWKRYRLVFLIVGVAALVGSTVASLLI
                     APKFRATAVFAPTRELQVPGTPLSDSLFVEFNNFGGAVEREQFLEVMASGAFRDTLIS
                     RMGLHSYWGLQTTGDKSMQAARNLYGKHVRVKPTQFIGIAVEVTDADPQMAAAVANAA
                     VGIADSMMHSLKSEVAAKTLQALEHQYRLALMELASLQDSFANLKKSFGLAPAVELSG
                     LRQAIAFARVQVTGDIPTCYVIDRAVKPSVKVSPNRGLIVVISTLSAIFFTIFVVAVL
                     ELARGSKLNQGD"
     gene            23682..25121
                     /locus_tag="PK839_05125"
     CDS             23682..25121
                     /locus_tag="PK839_05125"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOG72155.1"
                     /translation="MVTDEHKTKLAALFLGLAFVAINSLLVTKNILSFSLLPVILLLV
                     LGVVLVPVKIFYLVIALAPLSVPLSEFLPGRSFDLWLPTEPLIFALLLLTAIHLIQER
                     RFDTGLFGNPLFWAMLFYLGWLVITCISSELPLVSVKYTVLRIGYIAFFFYIPFVLFK
                     ATPQNFTKYIAFYITGLTVVVAITLYRQARIGIFDKFAAVGTCNPFYTDHTSYGAALA
                     FLIPVVVAWAASTKTLWQKILMGILILVFTVALVLSYSRAAWLSLLVAVLIWVVWLMK
                     LRFRTIAILSVSIIAIGFGLRGQLAQWVGKNQTVSTGSLSDQLLSVGNITTDVSNVER
                     LNRWTSALRMFRERPFLGWGPGTYQFTYAPFQPSQFFTVESSNLGAKGNAHSEYLGLL
                     AEAGWPATMGYIAILLVAFIMGFRLLGKLPLDFPYRKLLLGVLLGLSTYAFHGFLNNF
                     LDMDKIAALFWGYLAMIAAMNTAYRTSSS"
     gene            complement(25108..26355)
                     /locus_tag="PK839_05130"
     CDS             complement(25108..26355)
                     /locus_tag="PK839_05130"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOG72156.1"
                     /translation="MSIATSQNRLKLSTFKLNALLGMAQAISAELKTEELVDRFRSIL
                     NDDLGIDRILLYKKEEDRWELLLNSNCPQKVVEGISVERDLMAFNEITFVGVAENPLL
                     LELDVIIPIVQNNAPVGYVLIGDTSEYEKGVSATIRHLNFVQTLSIIIFVAIENLRLF
                     HKSLEQEAMRKELELASKMQSMLIPTPESMPHYPHISIASFYNPHFEVGGDYYDVIPL
                     NDNEFGFCIADVSGKGISAALLMSNFQANLRALFSLDIPLTRLVEKLNERVMATANGE
                     KFITLFVGRYNVEKHLLEYVNAGHNPPFMIDKQSKIISTLSNGCVGLGMLDCIPVINQ
                     GAVTIDQPSLLVCYTDGLVEIMRNNKVEYNTQVIEQEIVKLEPISTTIQRIAESRGIK
                     NDGSSNPEIFDDISILGFGFNCC"
     gene            complement(26463..27029)
                     /gene="efp"
                     /locus_tag="PK839_05135"
     CDS             complement(26463..27029)
                     /gene="efp"
                     /locus_tag="PK839_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HOG72157.1"
                     /translation="MATTADFKNGLCIEFNGKLYTIVQFQHVKPGKGGAFVRTKLKNL
                     ETGKVIENTFTSGVKINVVRVERRPYQFLYKDDLGFHFMHQETFEQVSLSGDLIENSD
                     LLKEGQYVEMMVEADKENILTCELPPFVEMEVTYTEPGLKGDTASSTALKPATVETGS
                     VINVPLFVNTGDKIRIDTRTREYYERVK"
     gene            complement(27194..28042)
                     /locus_tag="PK839_05140"
     CDS             complement(27194..28042)
                     /locus_tag="PK839_05140"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HOG72158.1"
                     /translation="MCPQGQKTGSFRINKNFELLRSRQGFVVLLACTLILGIPVASAQ
                     KAFGPGEKLNYKIYYGLIHGGEAVLEVRKGDYKGKPANHIYMAGKTVGLANKFYYVED
                     IYESYTDPETDWPYFSIRNIHENRYRHYSTQVWDHWSRKDSSIVNSSKTGEVVVVKGC
                     QDILSSFYYLRNILIKSDPVKDQLFIVDTYFTDEKFPLMVRFKGRETIKTSLGEFRCL
                     KFMPVVEVGRVFKTQDDMTIWLSDDDNLVPVRVRFEIFVGAFYCDLYSFSGLKNPFSS
                     YSPKVK"
     gene            complement(28002..28709)
                     /gene="tsaB"
                     /locus_tag="PK839_05145"
     CDS             complement(28002..28709)
                     /gene="tsaB"
                     /locus_tag="PK839_05145"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HOG72159.1"
                     /translation="MATILCMETGSTTCSVALGSESGVIGFREVSDANIHASALPVLI
                     EELIRETGFELGNIDAIAVSMGPGSYTGLRIGVSLAKGMCYALHKPLIAVGSLEAMVT
                     GVGEFINMESINNNSLLSPMIDARRMEVYTQLFNRKGEMLSEVKALVVETDTFSDLLK
                     KNTVVFFGSGASKCQQVIGNSGAIFVEHFKPSARYLLPLAQKKFRDELFEDIAYFEPY
                     YLKDFVPTRPKNRIIPD"
     gene            complement(28967..30289)
                     /locus_tag="PK839_05150"
     CDS             complement(28967..30289)
                     /locus_tag="PK839_05150"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOG72160.1"
                     /translation="MKKGLLKYLIGAMVIIIVLAIVGKKKGWFGSTDAIEVYVEKPQK
                     RTITESITANGKIQPEVEIKISSEVSGEIIELPVKEGNWVEKGTLLVRIKPDTYISMR
                     DRAIASVNSANARLAQAKAQLSQSELAFNRSKQLFEQKAIAESEFETAKTNYEVAKSE
                     LKASEFNVESAQASLKEAEENLRKTSIYSPISGTVSKLNVELGERVLGTIQMAGTELM
                     RIANLNRMEARVDVNENDIVKVNIGDTALVEVDAYLDRKFKGIVTQIANTANVQGVST
                     DQVTNFEVRIFLLDDSYKDLVTDSRLSPFRPGMSTTVEILTETRSNVLTVPILAVTVR
                     SDSTAKKDITGMEKVDDSSTPVEQTNSKLNKPKEIVFAVSGDTARIITVKTGIQNNQY
                     IEIVEGLKGDEDVVTAPYSAISRKLKDKSLVKKVKTKNDLLKAEVAKK"
     gene            complement(30413..31753)
                     /locus_tag="PK839_05155"
     CDS             complement(30413..31753)
                     /locus_tag="PK839_05155"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOG72161.1"
                     /translation="MKKIVLFALAMEVLSGMAQAQDPWTLEQCIQYANENNLQIKMGE
                     LSVNMSKDKLLQSKLSIYPSANLNANHAYSFGRVTNYITNQKESRNTQSTSFSFSSSM
                     PLFEGFQLTNTKKQSNYDLLASIQDVETVKNNIALSVASAYLQILYQQELVEVAQRQV
                     EQSEMQVKRTALLVNAGSLPEGNLYEVEALLASDELQLVNSRNQLDLAYLNLTQLLEI
                     KDVENFRIAKPNISEVDSIAVIQNPQEIFTVAQQTMPQILGSQYKVQSSEMGLKIAKG
                     ANYPRLTLSGSYSSGAQSYLNNDNPLLTSDPFFTQIKDNASTSVGLSLGIPIFNGGST
                     RYRVSTARISLDNARLTLENEKNILYKDIQQAYADAIAAQKKHKASQKSKMASEESFR
                     YTENKFNLGLLPAIDYTTARNNLAKAETDLLQAKYELVFKTKILDFYKGVPLSL"
     gene            32636..34621
                     /locus_tag="PK839_05160"
     CDS             32636..34621
                     /locus_tag="PK839_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004330529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HOG72162.1"
                     /translation="MFKNHPKGLLVLALTNMGERFGYYTMLAILTLYMQAKFGLTAAS
                     TSMIYGAFLASVYFLPIFGGVLADKVLGYGKTILLGILVMFIGYLLLAIPLEGNSVGL
                     TMMIISLVLIALGTGFFKGNLQALVGKLYDDKRYDTNRDIAFSIFYMFINIGAFFAPS
                     AANYINNAVMKSSDFTYDASIPKNYVVLNDEKQAVDKNTFIVQNLTPAEQQLSGKEFD
                     AVLTKAKKKHKVVFTEDIEHALFRLKAAGLNQYTQSGKITDPAMMISNNEYELLQSRS
                     QEDMLKKAEVTAKIQAISITDPALEGGPNPDDFARNFGKHYVSESLSTSYNLAFGVAC
                     ISLIISVLIFLLFKKSWKSVDKTVKQEIAESKANGKEVKVMSKEEVRERTVALLLVFF
                     VVIFFWMSFHQNGLTLTFFARDYTQSVISPGWTMLFNLFPLLSLIVFLYGIYLSTIGL
                     LGGKPNKKSGLLLTVLGIAGLAGYYFTRVKGIEVMTKITPQIFQQFNPMFIVILTPLT
                     VALFSYLGKKKKEPSAPRKIGIGMFLAAIGFTIMLICSFGLPAPSEISGVSDQLVSPA
                     WLISTYFVLTVAELFLSPMGLSFVSKVAPPQYSGLMQGGWLAATAIGNLLVGVIGTFW
                     EKLPLVGFWVILIICCVISGIFIFSVMKRLEKATGNV"
     gene            34926..35273
                     /locus_tag="PK839_05165"
     CDS             34926..35273
                     /locus_tag="PK839_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HOG72163.1"
                     /translation="MNIYVSGLSYRINDDDLRQLFEEYGEITSAKVITDRETGRSRGF
                     GFVEMANNEEAQRAIDELTGAEYDGKVISVSPAKPKTDKPRNGGGFNRERRGGFDRGS
                     RGGSGNRGERRDY"
     gene            complement(35402..35791)
                     /locus_tag="PK839_05170"
     CDS             complement(35402..35791)
                     /locus_tag="PK839_05170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72164.1"
                     /translation="MKTFKTILLVLVLLLIGAGAFLYFGVYEKGVMAGRILRVSQKGV
                     IFKTYEAKLSIESFGALRSTSPIAETFDFSVERKQKELIKQLEEVALTGERVNVHYLK
                     RYMRVPWRGNTKYFAESIERLGKTQQP"
     gene            complement(35850..36596)
                     /locus_tag="PK839_05175"
     CDS             complement(35850..36596)
                     /locus_tag="PK839_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018696781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOG72165.1"
                     /translation="MKALHSHRSIRRYKSTPIDESLLNEILQAGIRASTTGNMQVYSI
                     IVTKSQEVKQQLLPCHFNQQMVVEAPVVLTFCADFNRFNKWCGQRKAEPGYDNFISFF
                     TGAIDALLVAQNVCIAAEENGLGICYLGTTTYTADRIVEVLKLPKGVVPITTVVMGYP
                     DESPELTDRLPLAGVVHHEVYQDYSEGDIDQIYMNKESLQLTKKLLEENGKETLAQIF
                     TDIRYTRADNLLFSKVFLKTIEQQGFMNNK"
     gene            complement(36708..37664)
                     /locus_tag="PK839_05180"
     CDS             complement(36708..37664)
                     /locus_tag="PK839_05180"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOG72166.1"
                     /translation="MDISIVVPLYNEEESLSELAAWINRVMTLHNLSHEVIMVDDGSS
                     DRSWAVIQELRKQYPAIRGIRFRRNYGKSAALQVGFTAASGDVVITMDADLQDSPDEI
                     PELFRMIKEEQLDLVSGWKKKRYDPLLTKRIPSKLYNFTVRMVSGIKLHDMNCGLKAY
                     RNNVVKSVEVYGEMHRYIPVLAKQAGFRKIGEKIVQHHERKYGSSKFGWSRFINGFLD
                     LLSVTFVSRFGKKPMHFFGTLGTLMFFAGGVIAVVLVANKIWSQTHMLRYRPVTDQPL
                     FYIALAIAIIGVQLFLAGFIGELVSRSASDRNTYIIEEDTGA"
     gene            complement(37675..38229)
                     /locus_tag="PK839_05185"
     CDS             complement(37675..38229)
                     /locus_tag="PK839_05185"
                     /inference="COORDINATES: protein motif:HMM:NF025227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HOG72167.1"
                     /translation="MEEKKSNLNVTMTWGLVVGLALIILSLVLYIMNIYKAPTWVGIL
                     NYALIIGGIVMAQLKYRNNILDGYITYGKAVGTGVLVSLFASIIYAFYFIILTKFIDT
                     GYMDKVLETVEQAYAENGLSEDQIAAALEMSKKMMTPIMMALSTVFGFVFMGTLFSLI
                     TSIFIKKEAPLFGSESKEGETLSE"
BASE COUNT        10248 a         8657 c         8869 g        10551 t
ORIGIN      
        1 aggcggacaa gttctggaat ccaccagtag gcggacaagt attcgcttac ctttaaccct
       61 gttttagcca gttatgaaga aatacaaacc cctgaggaaa attataagca acaccggaat
      121 aaagaatgta agtagaaaac ggaaaaagaa gtagtttagg gtgccccggt aaattagcca
      181 cccaagcaaa accaccagag agctgtcgaa aatggaaaga agccggttgc tttttttcac
      241 caaaaaaact gatggaaaat tcagcacctc ctgtttatcc ccgctaacaa gcaaaataag
      301 gttatagagg catagtgtaa aaattacagg cgatactacc ccgaatatca gtagaattac
      361 gttggtttga gcgattttaa aggagtaaag cgtcccgaaa agaatagcaa tagccaggtt
      421 gaggataaac cgggaatttg tgcctacaag atttttagtc cagtagaaaa acaaattatc
      481 ctcgaagttc ttgttatcca ttatgtaggg aataagcagg tgtaaagttt taaatccagc
      541 aacagccaca cacagctaat tttcaaggtt ttttggctag ctgctgcaaa gataattgcg
      601 attaggttaa ctttgctaac ttaaacaaag gataaatata gatgctaaaa aaaattcttc
      661 tgataaccat ttttgctgtc gctctgcttt cacttttccc taactgtgcc aaggtggtaa
      721 ccccaacggg aggcccaaag gacaccattc ctccagcgct ggtaaacagc atcccgaagc
      781 ctaacgccat aaacttcaga ggggaaaaac tggtattaga gtttgatgag ttcctggcat
      841 tgaaggacgt gcagcaaaag ttgctggttt caccacccct gaataacaga cctcaaatta
      901 agccaaaggg caagcgaatt gagctggaga taaaagatac cctgctggag aacaccacct
      961 acacggttta ctttggcgat gccataaggg ataacaatga gaataatgcc attccaaatt
     1021 ttgagtttgc attttcaacc ggcccaacca ttgacactgc ttctctggca ggtactattg
     1081 tcaactcttt tactaatgaa ccagttgagg gagcgctaat catgctctac agtagttttg
     1141 ccgattccct accctatatt aaacgtccaa cacatatggc aaagtcgggt aaaaatggca
     1201 ggttcaaggt aaacaatctt aaaaacattg attacaagct tgttgccatt accgatgcca
     1261 acggtaacta cctttacaac cagggcattg aggatattgc atttgtcgag ggtaaggtaa
     1321 cacacgatat gctaatgaac gcttccgatt cgtctaggtg ggtaaggctg cgaacattta
     1381 gcgagccact gccctaccag ctacttacgg gtaccaaccg ccctcagcgg gatctaattg
     1441 agctaaactt ctcccgcaaa ccggttggcg gttttaagct ggtacaagtt gacaaccccg
     1501 ttagcgagaa atggtatatt cccgaacccg acaacgaggg agacaccgtt aaactttgga
     1561 ttatctcgga gaaaatttcg gctgctgaca cgctaaagct ggctttgtcg tacctcaaaa
     1621 ccgattccct caacatgctt cagcctcgga tagataccct taggtttgtt tactatgaat
     1681 ccgaggaagc cgggaatgac tcccgttcaa agcgcggaaa gaaggaggaa gttgtaaaac
     1741 caaccggatt caaggttgaa acctccatca agaacatggc agttgctatc cccggagttc
     1801 cctttgagtt tacccttcct tcacctgcaa gaaaggtgaa tggtgcgcta gtgcagattt
     1861 ttaaccaaac cgacagcatt ttggagcctg gcgtgaagct tgtcgcagac tcgataaacc
     1921 ccaggattta ccgtttcaac acctcctgga aacccaatgt aacctacaaa atgattgttt
     1981 tgccgggtgc ttttgaaagc tacacctcaa ccctgaacga cacccttaag cttcagctta
     2041 ccggagctga ccccgaaagt tttggaaccc tcacagttaa attttccggt gtaagtaagg
     2101 cggttgtagc tgagctgctg accgaaaagg atgcagtggt agataggaaa gccgttgtag
     2161 gcaaccaatc cgtgaccttt acgttcatag cccctgcaaa gtataaactt cgatttattg
     2221 atgatttaaa cgcaaacggt acctgggata gcggcaacta cttgaagcgc attcagcccg
     2281 aaagggtaat cccgtttact gaagggaaaa ataagggtgt gataaacatt cgggccaact
     2341 gggataacga gatttcggtt agcctgcaaa aaccataaaa tacctatgtc tgtactgatg
     2401 aattttgcca tgtttcccac cgataagggt gccagtgtta gtccctacgt ggccagggtg
     2461 gtggagggcg ttcgttcgtt gaaccatgcc tcacagctta ccgcaatggg aacaattgtg
     2521 gagaccagct cccttgagga ggccttgcag gtggtggaaa aagcttacca attgctcgaa
     2581 cccgattgcg acagggttta cgttacagta agcttagaca ttcgcaaggg aggctccgga
     2641 aggattagct taaaggtgaa atcggtcgaa gacaagctgg cttaggcaat tcttctacat
     2701 atccaacaag cctcagccat aatagcttca ttaccgctga tagtttcatt atgtgcgaag
     2761 aattgagtgg aagctttcat gataatatac ttgcatgcca atgtgtaact ttgaaatgaa
     2821 taaggctgac attgccaatt attttaaggg aagggtaata atttttctct tcttcatttt
     2881 gcttgtttcg gaaggcatta aggctcagcc atatgttggg ctaagggctg cttataccac
     2941 ttcaaccatg acttttaagc ctgatgctga aactaaactt ttaacgggta gcgggctgga
     3001 ctacggactt gaattcaagt acttcaatgc aaagtatgta gggtttcaat ccgagctcta
     3061 cctgaccagc aggggttatt taaaagctat tgatgttgag gagttgggtg atacactttt
     3121 taaaagggtt aacctctatc tggagttgcc catgtttatt cagttcaggt tgaagttggg
     3181 gatagtaatg ctgcatgtga atgccggtcc gtattttgcc tacctcctca gcgccaaaga
     3241 gggcgataac tccaccggaa agtacaggct gaacaaggtg gacatcaaca ttcttcgcga
     3301 aaaccgtttc gactacgggt tgatgggtgg tgtaggccta agccatgact tccaatgggg
     3361 aaccattcag gctgaagcca ggtttggtta cgggttgggt gatctttacg attactcgta
     3421 ttccggcatg ccaagcgagt caagggctat catacaaaat tttagcctga gctaccttta
     3481 ccgatttggc caacacaata cacagcaaga agtcaaacag ccttaagtta taattttctc
     3541 acctgaacgc aggagtgtgt gttttcaaaa atttactgta aactatttgt agttttgaat
     3601 gttactgctt tcgttgtggt aaacctatgt tttagtctaa cgtgcttgct tgccagggtt
     3661 aagcaacccg tgcttcagga ataattgctg cacactgtta aggcattaag gtaatttacc
     3721 ccctactagg cttatgttag ctgataaatc gtagtactaa aattatttcc gattacttca
     3781 tgaaagcatg gaaggaaggc gattatagat tacagcaggt gatttcgaat acccgactag
     3841 ccccgggagc gtttcttctg aaattcagaa aacagttcga ttttagtgca gggcagattg
     3901 ttgcccttgg agtttctacc gacattgaac caaggttcta cagcattgct tcttccgagg
     3961 aagacgagtt gatatcggtt ctctatacgg aaaaacccga tgggaagcta actcctatgc
     4021 tgtcaatgct caatccaggt aacccgattc tggtgtcaaa gccatttgga gcatttacca
     4081 gctttaaatc cgaaccggtg tttgttgccg ccggtactgg ggttgctcct ttcatttctg
     4141 cactcctatc caataaggtt agaggggcaa ctttaataca tggtgctcca tatcccgagt
     4201 acttttactt ttccgattat ctttccgaga agctaggaag aaactatatc cagtgctgct
     4261 cgaggtgtat gcccggaagc cattatgttg gtcgtgttac cagctacctg ctgcaatggg
     4321 atgagctaga tccaaaccga aattactacc tttgtggcag caccgaaatg gttgttgaaa
     4381 caaggagctt actaatttca aggggtgtac cacccgatag aataaacgct gaaatttact
     4441 tttaaaccta ttggtagctt ttcacaattc attaatttac aatttaccag aaaatcatac
     4501 tacctcgatt gtttccatta acttcttaat cttatgcccg aactactccc gctatatgga
     4561 aagacgcttg ccgagctaca ggatattgtt agccaggagg ggttgcctcc gtacgccgct
     4621 atgcagcttg ccgactggct ttacaaaaag aatgtggaga gcatcgaggc gatgaccaac
     4681 atttccaaga gcgccagaga acagcttgtc ggtaggtatt acgtcggcct tagcaagccc
     4741 gaaggcgttt cagcttcggc cgatggaacc aaaaaatacc tttttcggag cggaaatgga
     4801 aaatttgtcg aggcagccta tatacctgaa accggaaggg caacactttg cgtatcgtca
     4861 caggccgggt gcaaaatggg ttgcctgttc tgcatgactg gcaggcaggg ttttcaggga
     4921 aatctttcct caggtgagat tatcaaccag ctccggagca tacccgagtt taaaaatgtt
     4981 acgaatattg tttacatggg aatgggcgaa ccattcgaca acctggagaa tgttcttcgc
     5041 agcaccgaag tgctgacctc cgaatggggt atgggctgga gcccgaaacg cattaccgtt
     5101 tcatccattg gaattatccc tgcaatgaac accttcctat cccgaagtag ctgccatttg
     5161 gcaattagcc tgcattctcc atttgacgag gagcgtagga tgctgatgcc tattcagaat
     5221 gtttacccga ttgccgacgt tgtggaggag atccgtaagg ccaacattgc ggggcaacga
     5281 agggtatcgt tcgagtacat catgtttgat gggcttaacg acagtccacg gcatgttcgg
     5341 gaaatatccc gactgttgca caacttacga tgtagggtta acctgatccg gtttcaccca
     5401 attcctgaat cgtcccttcg cagcgcatcc gatgaaacga tcataaagtt tcgcgatgcc
     5461 cttacctcta aaggaatatt caccaccatc agggcatctc ggggactgga tattcaagct
     5521 gcatgtggac ttctatccac aaagggtttg ctgaaaaaca aggattgaag gtagcattcc
     5581 catcctggta aaattttaat tgggcagtaa agctggttaa gcgctatgct gtttttgata
     5641 aacgaagagc agcctcggtc attacgtcaa cttcaacagc caggtttatc agcaagtgct
     5701 ttcgaatggt tttatcgtgg aagtaaatgg aaacaggcga aaccccaacg ccataatccc
     5761 taaccagcat ttcggcaaat tcaacatctt ttaaatccga cacgtccgag tagtccaggg
     5821 tttgagtgta tccggcctgc atgggtagtg gtttgaacct ggtagatgaa agagaatcaa
     5881 gcagaatctg gcgatttttc tgaattacag catggtaggg gaaagaagtt tcgttggtgc
     5941 tcatgaaatc ggcaatagcc agctgtaggg cgtggttggc ggtgttggca atggctcgct
     6001 gaatacgttg aaagttgttc atcatggctg ctggagcaag gcagtaacct aagtcccaat
     6061 cggaaattcc cagggtttgg ctcaacgagc caattatgta tgcgttatcg gccaatttag
     6121 gaaaaaacgc cacgctagat ggaataacgc cagggtacac gatatcgctc aaagtttcat
     6181 cgctgataat tttaagcttt gttccgttta tcagcttttg caaattctcc atgtcgtcag
     6241 gcgaaaggat gcttccggta aggctgttag gagagcttat gattatgagc ttggtacgcg
     6301 gggtaaccga cttttgaact tcatgccaat caatacttgc gttatcctcg tgtagcttaa
     6361 gataaacagg tcgggcacca caaacctcga tggctagcgc tgtgtcgttg cgtgtgggct
     6421 cgaaaatgat aacctcatca ccatcactca ccaaagccgc aactgccgaa aaaattgcct
     6481 gggcagcacc cgaggtaatg gcaatttcag ctgcaggatg gtatgtccga aagtattttt
     6541 tctgaatgta ctcagaaaca tgctttctga gctctagcgt gccctctatg ggcgcacgtc
     6601 catgcgtttg cctcaactcc ctggcaacca aatcggccac ctgcactggt atgggaaacc
     6661 ttgtccatcc cgcagttaga tctatcgcat tatggctatt aataagttta agcaaggaat
     6721 ttgccgtatc aactccaaat tccgcgttaa tctgtttcat tcaaaagaaa aatttatcat
     6781 tgttttataa aaatactcaa gttgtgccac cccaccataa atgtttgaca aattgagcca
     6841 atttggtggt gaaagtgcgt tattgaaaaa gttgaattaa aattggaagc gttttaaaat
     6901 cgagagggga gccaaggtgg aagttgtagt agcacaacag gtcgttgatt gcctgaattc
     6961 tttgagcatg gtttagagat atttcatttg acctataaaa tggggtgcct agcagcattc
     7021 ccaggcgacg gcttgaagtt ttgtcgaggt tgaaagaatg ggatggtggt gaaggacaga
     7081 aaaagccatt tttatagtcg aaaaacggca tggtttcgct ccatccgttt gccgggtaaa
     7141 aacccaggta tctggttagc tggatgagaa aaatcaggtg gaagttggcc actccatcgt
     7201 gcatgacatc cagcagcctt atagaatttt ccaaaaattc atacagctct gagttgggtt
     7261 caacctccct aacggtgcgg taaatcacct cgctaacaaa cattgaaatg ctgattttaa
     7321 caggatcgaa gtgaagctcg ctcagaggta tggcaattgc cacttctttt aagagcgaaa
     7381 tttcggatgt ctcctttttg tagaacacca agtcaagaat gtcaagcggt tggaagtaaa
     7441 cagccagacc agcagttttc ccgctaccgt atgcgtggtt cacctgaatg gttgttcgac
     7501 caaactcctt gctgtagcat gtagctataa ggctgttatc acggaacttg accgcatgca
     7561 ggacaattcc ccttgatttc tgaagcatgg gttacctgat aaaaagaatt tttgtaacgg
     7621 cagttttttc gccctgacta gccgagcaaa agaccaggta aaccccggag gaagcccgtt
     7681 gtcccttgct gtttttccca ttccatgtag ccatacctcc aacagagaaa gtctcgtaaa
     7741 ccaggtttcc gcttatatcg gtaattttta ccagggtatt ctccattaaa ccggtaatgg
     7801 taatgtcacc ttcatattcg ggtcgaacgg gatttgggaa agcataaacc tttccaaagc
     7861 ccgatcctgg ttggttggca ttgcttcgaa acgaaaccag ccctttatcg gtggcaataa
     7921 aaacctctcc tgatgccgga tgaactttta catcggtaat cctattggac gggagagggc
     7981 tattctggat tgtgtagtgg gccaactcct tggttccgtc ggggctaaac aggaatactc
     8041 ccgaacgggc tgttccaaac catttacggt tacccccgtc aaccgttaca gaggttacct
     8101 cctcggtttc aagaaggtaa actgccagcc cctcaaccac atcgggaatt ttaatctttt
     8161 gaaagtacat gcccccatca aaaaccttac cggggttgaa gcacactaaa acaccctggc
     8221 ttgttcccag ccataggtag tcgtccctat caaatgccag ggcgtttata tcaacgttga
     8281 atagcgaccc gctgctactg gccggttgta gttttctgta gcggtcgtcg tccttgatgt
     8341 cgatattatt accagggttc agggcaaaaa gtccttgacc acgaggtagc agtagccata
     8401 gcgttccgta gttggagtag ctgagggttg acagcttgtc agaattgata gctgatgcat
     8461 agggaaatga aatccacgtt ccatctgtct ttcggacaga tatagggttt gcgaccattg
     8521 tgtttgccac ccacaggttg ccttttgggt caaaatccag gccgcttata cggcagtagg
     8581 gagcattggg gaaaatggac tgtagggagc tgttttcagg cgtaaagtgt gcaaccatgc
     8641 tgccatcgtg gaactggtat aggccgccac cccagctggc tacgtagtaa tctgatgggt
     8701 tgcctggatt aacccttacc gcaacggcat catgatagct ataatcagca aaactgctcc
     8761 attcctcatg cgtgaaatca tgaaccatga agggatgcca caagttggtg aaggttgcat
     8821 cgtagcctcc cgaggccaca actacacgat tgctgctaac atctatggca aggtggttgt
     8881 tgtttgcagg accgtttggg tagatagacc ttagcgcctg agaactacca aacataagac
     8941 cgttaaactt atcggctatg gccagcttgt ctgcatctac cgaaattgca gcttgtgggt
     9001 tgataaacag tgatgcgggt gaaagaaatt catctctaat ccccggaaat tggtaggttt
     9061 gaatcccatt agcggaggtt accagaatcc tattattgcc tacgctaaga tgaagggtgg
     9121 caggcaaagg ccggtcaact tcaacccatg agcttccatc gaatcgccag agaatgtcgt
     9181 tggcgttctc ctgtctttcc agagcaaaaa ggtagccatc cttagcagca actccagcgc
     9241 attcggcagt agggttggta aacgatgttt cagtagtcca atgctcaaag cttaccagaa
     9301 gagggtcgga gcacagggca cttttcaggc cattggaagt tgcagcccag aatctatccc
     9361 caaatacctc catgctgctc acccggatgc cggttccatt gtttccgata tagtaggttt
     9421 cctttatttc ccttttagct ggatcaacca ccactagtcc aaaatcggtt gaagtgtaaa
     9481 tcaggttgtt aaaaagggta aaccggttaa tggttttact acccgaaagg ggtttttcct
     9541 ttataaaggg aaggttaact acgctgttct gaaccagcaa atctatgttt ccactttcgt
     9601 agccaatggc aagtatttgc aatgaaggga aatagctaag tgccgagatt ccaacttccg
     9661 aaaggccatt caccctcgta actttatcaa cctcaccggt ggctatattg tatgagaaaa
     9721 ttccgttatc agctccgcag tatatcttcc cttcttcgta gcacagcaat tttatgttac
     9781 caaacgaaaa gtggtcgcgc cagctaccta ccggaatctg tgcttgcagg actccctgca
     9841 gcaaaaaaag gaataaaaaa accgcactgg ttactcttgc cattcagctt taattagttt
     9901 aagggcggta gcagtaaagt ttaagacaga atttactgtt ttttcaaaaa atcaacgagt
     9961 ttggatatag ccatacccct atggcttatg gagtttttaa gcgcgagggg catttgggca
    10021 aaggtttggt tgtagccatc ggggatgaaa accggatcgt atccaaaacc gtcaaagcca
    10081 ttgggggagt caattatttt tccgttgaca gttccctcaa aaaggtagtc ctttccggag
    10141 agtgtcaggg cgataacagt acggaatctg gcgttacggt tctcaacccc gttgagtttc
    10201 atcagaagtt ttttaatgtt ttcggttgat tttttgtctt cgccagcata tcgagcgctg
    10261 taaacgccag gttcgccatt caatgcctct acttcaagtc ctgtgtcgtc ggcaaagcag
    10321 ctgtaccctg ttcttgaaaa tacgtaacgg gctttttgca tggcattttc ctccagggtc
    10381 tcatagtctt cgggtatttc ggcaataaat ccaatatcat gtagggatag cagctggaaa
    10441 gcatttccaa gaatggcctg aacttctttt agcttataga cgttgttggt ggcaaaaacc
    10501 agtttttgtg gaatgttaat catacctagc gtggattgat gcacataacc gggatgtggc
    10561 cagcattggc aagaatgtac tgctccatgg gatcaaccgt aaaccgccct tcgctgtcaa
    10621 ggggtgtagt cataagaaca atcagatcgg ccctaatgtt ggcggcaagg ctcaaaatct
    10681 cctgctcgta aggttttaac ccgggaacag tatattcaac agatttagcg cccttttggt
    10741 caaggaatct acgtgccgaa gccatgttga tgtcaatctt ggccagcagt tccggatcgg
    10801 tagtgttggg tttaaccaga tgaaacctcg acaggtaaaa atcgctgaag taggatatcc
    10861 acttgtgctt ttcctggttt tcaagggtaa aatcaacagg aaaaaccacc tcgtcgtagc
    10921 gtttgtttac cgggggctcc tgaatggtaa tgaagggcac cttgcagctg gtaatcacct
    10981 tgagcaccca tgaaacttta ttttcatcca tttctttaat tgtggatgag cccatgacaa
    11041 caaagctggc ttgtacctca tcggccacca gggctatgtc ggtgaaaatg ttacctaccc
    11101 gaaccaccgg ggtgatgtca actccgtact tctcctttgt gccccgaact attttctcca
    11161 gcctggcctc tgcctctgct ttatcggcct ctgctttaac aatatgcaaa atagtgatag
    11221 gttctttcac tacgccggaa accctaacgg catgctgtag ggcatactct gccacctgtg
    11281 aaaagtcggt agggaccaga atggttttac tttctccctt catacttaca tttttaaaat
    11341 gttcaaggct ttagccggac ttccaaagtt ttataaaatt ttcatacaaa aataataaaa
    11401 cctttaaaac ttgggtttct tttaacggat tcgtggttat tttaaacccc ggggttgcgg
    11461 cataaattac tgaaccatga atctggaggg gcaatgtgtt ataagttaga gagaagagaa
    11521 aaaatcagtc caaaccagat ttcctttaat ggcttttatc ctttaacctt ttacatacct
    11581 gaatatgcca acgagacgaa taaatattac ctgattaatt tcaaatcatt taaaaatatt
    11641 gtagcttaga tggcaaatct tcctgcatat tactgaataa tgatttggct tggttcaata
    11701 ctagtgttat taaccctttt tttgctctat agcttgataa gctactcgcg aacaaaggcg
    11761 atgcatggct tggagaccct actatttcaa cgtactaatg agcttttgaa ggagagggaa
    11821 aaatcggaaa atcttctggc ccgtgtacta cctcaggaaa cagccgatga gcttaagcaa
    11881 tttggaaaga ttaaagcgca aaagtttcag atggtaaccg tgctattctc ggatattcaa
    11941 ggttttacca agataaccga cgacacggat tccgaagagc ttatcgatca gctcgacagg
    12001 tttgtttacg agtttgattc ggttgtacaa aagtaccata ttgaaaagat taaaactgtt
    12061 ggggacgcct acatgtgtgc cggagggctt ccgaagaaaa accgaaccaa ccccattgaa
    12121 gtggtggttg ttgcgcttga aatgatgagc tggatgaagg aaactaaccg aaggcaccag
    12181 aaaggtgatg tgtgggagct acgtatgggc atagacaccg ggccggttat agctggagta
    12241 gtagggcaaa gtaaaatttc gtatgatatt tggggagcaa ccgttaacac tgccagcagg
    12301 atggaatcgg ccggcgaagc tggtaagata aacatttcgg aaaataccta cctgctggtg
    12361 cgggattttt tcatttgcac ctaccgtggg caaatccccg taaagaatag ggggaacata
    12421 cagatgtact tcgtggaggg tattctgccc aacctatccc aaaacctcga aggcacactt
    12481 cctaaccagg actttttcat cgagctccag ctaatccggc taagcgacct tgaagagctg
    12541 gtgctggaga aacttgagca gggccttcca aaaaatctat actaccacaa cgtcaagcat
    12601 acggtggatg tgtacaccca ggttgagctg attgggcgtg cagaaggggt tagcaaggag
    12661 gatctgctcc tactccgaac cgctgccctg tttcatgacg caggccacct ggttgactac
    12721 gccacccacg agcaaatggg ggtaaagcta gccagggaaa ttctacccaa ctaccggtac
    12781 tcttccgacc agatagatga aatctgcaag ctaatcatgt caaccaagct accccccaat
    12841 ccgaaaaacc ttcttgagga aattatatgt gatgccgacc tggattacct cggacgaacg
    12901 gactttatcc ccgtttccaa catgctttac aaggagatgc acgaaaatgg aatgattggt
    12961 tcccttcacg aatggaatat tctgcaaatg aattttattc aaaaccacca gtacttcact
    13021 caaaccgcac gaaggctaag gaatgtaaac aaggacatgc agcttcagaa catcaagagt
    13081 tggataaacc aaatggaaaa atccaacctt tagctatgat ttttacacaa aatattttac
    13141 ttttactgaa tttattcaaa tgtaatggga attaatcact ttggctttta ctcgcaattg
    13201 ttgagcatga atgagtatgc cattttataa acaaaacatc tcggtatgga tagcataaga
    13261 aaggtagttc ttgttccttg ggatttcacc caaagttccg aatacgctct tcaacatgcc
    13321 attgagctgg cgaaggctgg agataacgaa attatgctgc tgcatatcat tccccattcc
    13381 ccactctttg acatcttcta caaaccgaac ttcaaaaaaa ttgaggcaat caacaagcaa
    13441 ttcgagaagg ttgcacagga tgtgcagagc aggtttggaa ttatcccgca aaccttggta
    13501 atggagggaa gtccccgtaa gataatcaga gggttaatac tcacggcaaa cgtgaacctg
    13561 attgttgccg aacatggcta caccattaag gataaaacct accgcacctc cgatttcatt
    13621 aagaatttaa cctttaaaga tattaccctg ccctttatta ttgtaagcaa gcccccaagg
    13681 cacgaccact atatcgaaat tgtaatcccg gttgattacg accgcaagtt caaggaggct
    13741 gtacactgga tcatctacct ggccaaatac tacaagtgca acatcaacct tattaaaccg
    13801 tttcttaccg atgacggaca aaagaagcag ctggccaaca acatctactt taccaaaaag
    13861 atgctcgatg gtgtgggaat tatttacggc attaaaaccg ctaaaaagaa aagttttttt
    13921 aaggacgagg tattccgctt tgccaatacc attgaggccg atttgatact ggttatggcc
    13981 gataagtaca actattttgt tgatgccaaa agggaggcca acctggacat ccccgttatg
    14041 tgcgttaacc ccaggattcg taagtatcaa agtttttact aatctctacc acacatcggg
    14101 ttatgatgaa gagtaaacca aacgcaagga aactcaacct tcagtttttt tcatcgcttt
    14161 cacttaaaag taaaattctt atcctgttca ccgggtccac tattcttatc cttatagctg
    14221 ctcttatttt catcagcaat cagctaaaaa gacaatctgt tgaaaacgcc attgcccaga
    14281 taggcattac tgccgatcag caagccttga tgattcaaaa tgccctagaa aagaatctgt
    14341 atattgccag aaccctatcg ggaactgcca aatcctgcag tcaactgcca cccgataggc
    14401 tcaacagcta ccttacttct gcttaccggg acgtgctgca atcggaaccg aatatcctat
    14461 ccgtttggga tagctgggag ctcaaatttc ttgacccggc tttcagcaac agctacggta
    14521 gaatcaggaa catggcatgg aggagcaacg gaacaatact caccaaatcg gagaccattc
    14581 ttactcctga ttcaccccag tacgcccgaa tcaaaaaaga gctaaccgaa tcggttgagg
    14641 agccctattt tttttctttc gataacggct cccctgtgct gatgaccagc ctggttgtgc
    14701 ccaacatcat tgatgggaag tttgccggag tgtcgggtgt cgacatcccc ctcactggtt
    14761 ttaacgagct catcagaggc attgaccgct atgaagggag ctatgccttt gtgctctcaa
    14821 ccaccctgaa gtttattgcc catcccgata agaacttggc aggtgatgac gccatggcca
    14881 gctatgagga gttgtttacc ctgcatggag taccagaaat tattgccaac ggcaactctg
    14941 ccttttttga gggtgccgat gctagtggag caaattcatt tgtggccatt cgtccaatca
    15001 taattggtag ttgcccccag agctgggcgt tggtgttgct tgttccaaaa ggtgttgtgc
    15061 tgcatgatgc caccgtaatt ttttggaaaa ccctgctggt tggcctggca ggcattctgt
    15121 tgctcacctt gctgatacag gcagtagcca ggaaatacat tctggagcct attattcacg
    15181 tttctgaaaa ccttagccgg ctggcaaaag gacatgtgga tggcagcatg aaggtgcatg
    15241 ttgaggttag cgatgagctg ggggccatgt cggaatattt gaatagaaca gttgacggtc
    15301 tcaccaaaaa aactgagttt gcccaaacta tcgggcaggg taactatgga gctaaaatgg
    15361 agttgatgag cgatgaggat gcccttggcc aatcgctaat agacctatcg gctaacctta
    15421 aaaaggcgca ggaggaagtt caaaaacgag cagctgatga ccaaaagcgg caatggacta
    15481 atgaggggct ggcaaaattc agtgaaattc tccggcagaa taacgatagg ctcgacctgc
    15541 tgtccatcaa catccttcgt aacctgattg actaccttgg ggctaaccag ggtggcctgt
    15601 ttgtttacaa cgacgacgac ccgtcaaacc cattcttcga cctactggct gcctatgctt
    15661 acaaccgtca aaagttcatt aagaaacgga tactccctgg cgagggttta atcggaacat
    15721 gtgccattga gaagcaaacc atttacctca ccgaaatacc agaaaactac atcaccattt
    15781 cctccggact tggagaagcc aaaccccgca acctccttat tgtgcccatg aaggttgacg
    15841 aaaacgttct gggagttata gaaatagcct ctttctcagc cataatggac taccaggtag
    15901 agtttgtaga aaagttagct caaagcatag cccaaaccct taccaccgtg aagacaaacc
    15961 ttaaaaccgc cgaactgctc gaaaaaaccc agcaacaaac agaggagatg aaggctcagg
    16021 aggaggaggt aagacagaac ctggaggaac ttgccaccat aaaggaggaa ctcgaaaaac
    16081 gcaacgagga cattcttgaa aaccagaaac agctcgagtg ggaaaaatcg ctactcgact
    16141 cgctgctaaa ctacgtcccc gacaaaatat acttcaaaga cctgaagagc cgcttcatca
    16201 aggtaagcag gtcaacccta aaagcccacg ggttcgagaa cccggatgac ctgattggca
    16261 aaaccgactt cgaccttttt ggagaggagc atgcgcgtcc cgcctacgag gacgagcaga
    16321 acattatacg aaccgactct cctattattg ggctggtgga gaaggaggtg tttaccgacg
    16381 gcagggtatc gtgggccgaa acttccaagc tgcctcttaa gaatccaagg ggagaaacta
    16441 ttggcacctt cggaattact cgcgatatta ccgcatcgaa gctcatggag gaagaggtta
    16501 agaaaaagga aatggatgcc cttgcatacc agcgcgacat gcagaaaaaa gccctagagg
    16561 cgcaaaacct ctacgaggca ataaacaact ccacctacgt tatagagtac aaccccgacg
    16621 ggtacatcac ctttatcaac gacgcttacc tggagctcct caactcgaaa agggaggatg
    16681 tggtcggaaa gcaccattcc taccagctcg attttaccga tgagcaacgg aaaaactaca
    16741 agcagttctg ggacgattta aacagcggaa tcgcccgcaa ggagactcat cggttcaccg
    16801 ttggtaaaag aacatacctc tttttcgaga cctatacacc actaaaggac gagaacggaa
    16861 aagtttttaa aatcatgaag attgcagtca acgtgagcca cctgctgcat gagggcgagt
    16921 agaagctgca taaatgaaac ttttaacagc accttccgcc cgtaaacttt ttcccatcat
    16981 ataaggtgca gcgtttatgc gaatactacc ttaataattt acctaccttt acccgaatcc
    17041 tttagggcaa ggggcgaatt ttagcattat gggaaaatgg aggtagattt tcccgaataa
    17101 ggtattcctt tttctgccca aaaggtagtg ccaaacatca gtcaatttat gccaacaaat
    17161 ggaatggaca aaactactcg atgccagccg gtttggcgca accggggcaa ctaaaaacac
    17221 ttacgatacc gcccgaacca actaccagca cgacttcgac cggctaattt tttcctcacc
    17281 cttccgtcgg ttgcagaaca aaacacaggt attccctttg ccgggaagca tatttgttca
    17341 caaccgcctt acccatagcc ttgaggtgtc gagcgtgggc cgatcgctgg gacagaatgt
    17401 ttacctgcag atatccgaaa cccttagtcc acatgaaaaa ccactggttg ccgaaatacc
    17461 aggcatcatc gcagctgcat gcttggccca cgacatgggc aacccgcctt ttggccacag
    17521 cggtgaggag gccatacgaa agttttttga ggacaacagc ggggttttca aaccgatggt
    17581 ggacagctgg gaatggtccg atttggtgcg atttgagggc aacgccaacg cccttaggct
    17641 ccttacccag cagctaaccg ggcggcggac aggtggattc aggcttagca atgccgtgtt
    17701 ggcttcagtg gttaaatatc cctacccatc agcttcgggc aaaaagaagt tcggtttttt
    17761 taagtcggag gccaatccct ttctgagtat tgcacatgca acaggaatgc atagtgtagg
    17821 caatatggaa ttttgccgcc atccgttggt ttaccttgtc gaggctgccg atgatatctg
    17881 ctaccagatt atggatattg aggatgccca taagctgggc atcctttcca cgccaatggt
    17941 ggttgacctc taccgtgctt tttttaccga tgagcccaaa ggtgcaaggg atagaattac
    18001 ctctacgcta aaggaggttc aggatcccaa cgaacaggtg gcctacctta gggctattgt
    18061 catcagcaag ctggtggagg agtgtgctcg gatatttgtg aataacctga agccaatcat
    18121 ggatggagaa gacttttcat cgttgctcga aaaaatatcg ggagcaagcc atacggctat
    18181 ggaggctatt aaaaacctgg cggtggagag agtttacaac cacaggaagg tggttgagat
    18241 tgaaatagca ggctaccaaa tcctggggtc gctgctcgaa attttctgcg atgccgttct
    18301 ccatccacaa accgggctat ccaaaaaggt catacggctt gttccaaacc agtacatgac
    18361 ttccagtggt gatgactaca ctaagattat gtcggtactc gactttgtgt cggggatgac
    18421 ggatgtgtat gcgctcgaaa cctacaggat cattaagggg ataaacctcc caacacttta
    18481 ggggttactt gttgcggtcc gtaacgtaat cggtgagaag catcagcgat tggcggtaat
    18541 ccgaatcggg aaagctgttg agcacttcct gggcttgctg cttgaagttt agcatctgtc
    18601 cggtggcata gctaattcct ccatattcat tggtaaactt taccactgtt gaaatggtaa
    18661 cgctgctttt acttctgctc ctgatttcac gcagtacttt tctgccttca gcagcgggtg
    18721 cctccctaag cgcataaatc aagggtaggg ttagcttctt ttccttgatg tcgttgccgt
    18781 tgggtttacc aaccaggcct ttgggctggt aatcgaagag atcgtcccta atctggaagg
    18841 ccattcccag gttaatacca aattcgtgca ttcgccttat ggaatcggaa tcggcaccag
    18901 ccgaaattgc cccattctca cagcacgagg ctataaggta ggccgtcttc ttacggatta
    18961 tatcgtagta agcctcctcg ttaatatcca ttttccgtga gtgctctatc tgcagcaact
    19021 ccccctcgct catctcccta acagcgttgc tcatcacctc caacaactcg tactccttat
    19081 tctgaacggc caccagcaag cctttggaaa gtaaaaaatc gccaaccaga accgctacct
    19141 tcgacctcca gagggcattg atggaaaaag cgcccctccg ctcaaacgac tcatcaacaa
    19201 cgtcgtcatg aataagcgtg gcagtgtgca gcagctcaat cataccggca gcaacatagg
    19261 ttgactggca aatcttgccg ttcaacccag ccgatagaaa tacaatgatt ggtcgtagct
    19321 gcttcccctt gttgcggtat atgtagttgg taatcaggtc gaggaaacga atgtttgagc
    19381 ccatgttctt cctgaaaaag ggttcaaatt ttttcagctc ctcgtcaact ggctgtttaa
    19441 tggcttcgag cattcctgga atgttgaaaa tattaagcgg taaattaaca ctttttttgg
    19501 ctattcattt aacgctaaag ctcaataaaa gtttagccga catttggcaa atgccatttt
    19561 tgttgatttt cccttgttat tacatgaagt tccgaataca cttatttttt attagtgcct
    19621 ttcgcctgta ctaacctttc aacttgctcc tcctcggggt tgatattcga caactggtag
    19681 aatgtggaat tgggtaaata cctctgcgaa gctggtgagg aggcttggct ggtaaataca
    19741 ggccgggcat aaaattctgt ttgtagtaaa atattaatat atttgcatgg atgtaatatg
    19801 aaacaccttt gtgagtaagc ctgaataaaa aaggacgaac aaccgagcgt aacttgcgga
    19861 ttcggcgaat ccaaacaatg ggtgcttcat ccgaaggtta acaagataga aatgccaaag
    19921 cgtggctaat tgtaaattta tagcactaaa ttttgtgcgg atggaactat taacagtatt
    19981 ttttaaatgg taaagataaa tgagttgaag gttgagcagc tcgaagatgc cgccaacatg
    20041 ctaaaggcaa tggcccaccc tatgcgcata gccatcatca gcttactgga ggctaatggg
    20101 aggcttacgg ttacgcaaat tcatgaaaaa ctccaaatag agcagtcaac cgcctcacac
    20161 caccttggaa ttttaaagga taaggggctt ctcgcctgca agcgcgaggg aaagaacaac
    20221 ctgtactttc tgcgtaacga gaatttggga aagattttgg attgtgtggg cagctgcacc
    20281 tccacaaggt tctaatccta acaacccttc taatggcagt atttgacatt gttgtggtaa
    20341 ttctcctggt tgtgggcgcc ataaggggat taaggcaggg attcctgtcc cagctggcat
    20401 cggtagttgc tcttctactg ggtatttggg gtgcagtacg tttttcagac tacatggcca
    20461 ccctgctttc ggaaaagttt gacattggct cggctcatct ccccatcatt tcgtttgccg
    20521 taaccttcat tttaattgta atagccatcc actttattga aaagatagtt gaaaggtttt
    20581 tgaatttggc catgctgggg ggaattaata aagtgcttgg gtttgttttc ggctttgcca
    20641 agtacgctat tattaccagc gtgctgctgg tttttgttga aaaggctaat atacgtttca
    20701 acttcttcac cgaggagcaa aaatcaaaat cgctggtgta caatcctgtt gcaagccttg
    20761 ctccggcaat attcccctac cttcatttcg acacaattaa ggaatcggtt aaaaaatccc
    20821 tcgggaacca gaagggtaag taggttcgac cttccttttc acctacactc gacattctgt
    20881 agttactttt gggacaaaaa cgcttatatt tggacgtttt tttgatattc ctgcaccgga
    20941 ctaattttac aatagaaatg aatttcatag atgagctcca atggcgcgga ctggttcacg
    21001 acgtgatgcc gggaacggcc gatcagctga ataaggagat gacctcggga tatataggat
    21061 ttgatcccac tgcggagtcg ctacatgtgg gcagcttcgc ccagatcatg ctgctgaaga
    21121 ggtttcagct atgcgggcat aagcccattg tgctggttgg aggcgctacc ggaatgattg
    21181 gcgaccccag cggcaagtcg caggagcgaa acctgctgga cagcgccacc ctacagaaaa
    21241 acctggaggg tatccggaat cagctttccc ggtttctcga cttcgacagc ggtaacccca
    21301 acgcagctgt tatggtgaac aactacgact ggatgaagaa tttctcgttc ctcgatttca
    21361 tccgcgatgt tggcaagcat atcaccgtta actacatgat ggcaaaggac tcggtaaaga
    21421 agcgatttgg cgaggaaacg aaggagggca tgtcgttcac cgagttttcc taccagctgg
    21481 tacaggccta cgattatctt tggctctaca agaacctggg ttgccgtctg cagatgggtg
    21541 gttccgacca gtggggtaac atcaccacgg gggttgagct catccgccgg atgaccggtg
    21601 gcgaagcttt tgcacttacc atccccttga ttaccaaggc cgacgggagc aagtttggaa
    21661 aaaccgaaag cgggaacgtg tggcttgact caaaacttac gtcgccctat aagttctacc
    21721 agttctggct caacacctcc gacgacgatg ccgaaaagta cataaaggtt ttcaccctgc
    21781 tcgaccagtc aaccgttgat gcccttgttg aggagcaccg tagggaaccc catttgcgaa
    21841 agctgcaaaa aatgctagca cgcgaaatta ccattatggt gcactccgag gaggattacc
    21901 gaaaggccgt ggaggcctcc gagttgctat ttggtaatgc caccaccgaa accctgatgt
    21961 ccatggatga ggatacgttc cttagcgttt ttgagggtgt gccattattt gaggttagcc
    22021 ggcagcatat tgctgaagga gtgccccttt caacgcttgc cgttgagttg gcacccattt
    22081 tctcatcaaa aggagaacta cggcgtctgg ttaccggcgg tggacttagc ctgaataagg
    22141 taagggttga aaacacggag cttccggttt ccaacagcca tctgctaaat ggcaagtaca
    22201 tcctcattca gaagggcaaa aagagctaca gcataatacg tacggtataa ggtttaaccc
    22261 cctatttttt tgacacttta cttaaaacgg gttaggacgt ttttcctgta agcttgatta
    22321 agcaaaggca gcgcatgctc cgaatttgtc aaagatagta agagcgaaga aaagccgggt
    22381 atttttgatt ggtgctatct tccaggccta gtccaaccta cgacgggtta aatggcgccc
    22441 tccctaccat acgtggtatt gcctaaagcc aagtgcaaaa acaatggcag aatttgcatc
    22501 ttcatggaat tgctaatatc ttggtgccgt ttgttgtaaa catcccgcca gaccatttta
    22561 attgcctcta tgctcatctt taaaatatgc tgtacgcctt cttccttttt ggcaattcct
    22621 tctaaaatgt tcgtaacttg cccgcgtgta aagtttaggt ttttcatact tttaagtttt
    22681 atggtttgaa actataagtt tatgaaaaac ttgctttaca cactttttgg gacagtatct
    22741 attattaatg ccgaatatgg aactttagca ttttaaattt tcgagttctc atcaacccta
    22801 acccatggag caggattata gaactgcaga accaagtttc gtggcaacgg taaaggtttg
    22861 gaagcgttac cgtttggtat ttcttattgt tggcgtagca gcactggtag gctcaaccgt
    22921 tgcctctttg ttaatagccc cgaagttccg tgctactgcg gtatttgctc ctacaaggga
    22981 gctacaggta ccaggcactc cattgtcgga tagcctgttc gttgaattta acaactttgg
    23041 gggagccgtt gaaagagagc agtttcttga ggtgatggcc tccggtgctt ttagggatac
    23101 gctaatctcc cgaatgggtc ttcacagcta ctgggggttg cagacaacag gcgataaatc
    23161 catgcaggct gcccgcaacc tctatggcaa gcatgtaagg gttaagccta cccaattcat
    23221 cggtatagcg gttgaggtta ctgatgccga tccgcaaatg gccgcagcag ttgccaatgc
    23281 agccgttggt atagcagaca gcatgatgca ctccttaaaa tcggaggtgg cagctaaaac
    23341 ccttcaggcg cttgaacatc agtaccggct ggcgttgatg gagctggcaa gtttacagga
    23401 ttctttcgct aatctcaaaa aaagttttgg gttagcccct gccgtggagt tatcggggtt
    23461 aaggcaggcc attgcgtttg cccgtgtgca ggtcacaggt gatataccca catgctacgt
    23521 aattgaccgc gctgttaagc ctagcgtaaa agtttcccca aacagggggc tgatagtggt
    23581 aatatccacg ctatctgcaa ttttttttac catttttgtg gttgcagttc ttgagctggc
    23641 aagagggtca aagttaaacc aaggcgacta atcccgtggc catggtaacc gatgagcaca
    23701 aaacaaagct ggctgcactg tttctcggtc ttgcttttgt agccataaac agcctactgg
    23761 ttactaaaaa catactgtcg ttcagcctgc taccggttat cctgctgttg gtgctgggag
    23821 tagtgcttgt ccccgtaaaa atattttacc tggtaattgc cctggcgccg ctatccgtcc
    23881 ctcttagcga gttcctgcca ggccgttctt tcgacctgtg gctgccaacc gagcccctca
    23941 tttttgctct acttctgtta accgctattc atcttattca ggagcgccga tttgacactg
    24001 gccttttcgg taatccactc ttctgggcca tgctcttcta ccttggatgg cttgtcataa
    24061 catgtatcag cagcgagtta cctttggtat cggtaaagta cactgttctg cgaattggtt
    24121 acatagcatt tttcttctac attccatttg tactgtttaa ggcaacgcct cagaacttta
    24181 caaagtacat tgccttctac ataaccggat tgacggtagt tgtggctata accctatacc
    24241 ggcaggctag aataggcatt tttgataagt ttgctgcggt tggaacctgt aatccattct
    24301 acaccgacca cacctcctac ggtgcagcgt tggcgtttct cattcctgtt gtggtggctt
    24361 gggccgccag cacaaaaacg ctatggcaga aaatattaat gggaatactt atcctcgttt
    24421 ttaccgttgc gctggtgcta tcctatagcc gggcggcatg gcttagcctt ttagtagctg
    24481 tacttatatg ggtagtatgg cttatgaagc tgcgatttcg tactatagcc atcctttcgg
    24541 taagtattat tgcaataggt tttgggttaa gaggtcagct ggcccaatgg gtggggaaaa
    24601 accagacggt atcaacgggc agcctaagcg atcagctgct ttctgttgga aatattacaa
    24661 cagacgtgtc gaacgttgag cggctaaacc ggtggaccag cgcattgcgc atgtttaggg
    24721 agcgtccgtt tttgggttgg gggcctggta cataccagtt cacctatgcc ccatttcagc
    24781 cttcgcaatt ttttaccgtt gaaagttcaa acctgggggc aaaggggaat gcccatagcg
    24841 agtacctggg tttgcttgcc gaggcgggct ggcctgccac tatgggatat atcgcaattc
    24901 tattagttgc ttttattatg gggtttaggt tgctgggtaa gctgccgctg gattttccct
    24961 accggaagtt attgctcggg gtgttgctgg ggctgtcaac ctatgctttt cacgggttcc
    25021 tgaacaactt tttggatatg gacaaaatag cagccctttt ttggggatat ctggctatga
    25081 tagctgctat gaatacagcg tataggacta gcagcagttg aacccaaagc ctaaaattga
    25141 aatgtcatca aaaatttcgg ggttgctgct gccgtcgttc ttaatgccac gcgactcggc
    25201 tatacgctga attgtggttg atatgggctc aagcttaaca atttcctgct cgattacctg
    25261 tgtgttgtac tccactttgt tgttgcgcat aatttccacc aggccatcag tgtagcaaac
    25321 cagcaaggac ggctggtcaa tggtaacggc tccctgatta ataaccggaa tgcagtcaag
    25381 cattcccaag cccacacagc cgttggaaag ggttgaaatt attttgcttt gcttgtcaat
    25441 catgaagggt ggattgtgcc cggcgttgac gtactccagg aggtgcttct caacgttgta
    25501 tcgcccaaca aatagggtaa tgaatttttc gccattggca gtagccataa ccctttcgtt
    25561 tagcttttca actaatcttg taagggggat atccagtgaa aaaagtgccc gcaggttggc
    25621 ctgaaagttc gacatgagca gggctgcgga aattcccttg cctgaaacat cggcaatgca
    25681 aaagccgaat tcattatcgt tgagggggat aacatcgtag tagtcgccgc ctacctcaaa
    25741 atgggggttg tagaatgagg caatggaaat gtgggggtag tgtggcatgg actccggggt
    25801 aggaattagc atggactgca tcttggaggc aagctcaagt tccttccgca tggcctcttg
    25861 ctccaaactt ttatggaaaa gcctaaggtt ctctattgcc acgaaaataa tgatggaaag
    25921 ggtttgcaca aagttgaggt gcctgatggt ggcgctaacc cctttctcgt actcgctggt
    25981 atcgccaatc aggacgtaac ccaccggggc attgttttga acgatgggaa tgattacatc
    26041 aagttccaat agcagggggt tttcggctac tccaacaaag gttatttcgt taaaggccat
    26101 caagtcgcgc tcaaccgata tcccctcaac cactttttgc gggcagttgc tgtttagaag
    26161 caactcccag cgatcctcct cttttttgta gagtagtatc ctgtctatac caagatcgtc
    26221 gttaaggatt gatctaaacc tgtcaaccag ctcctcggtt ttcaactcgg cgctaattgc
    26281 ctgggccata ccaagtaggg catttagctt gaatgtgctt aactttaacc tattttgcga
    26341 ggttgctata gacatggttt aaaaaagtag cccaatccaa cttggaaaag tggaataagt
    26401 taacaataaa aaaaggggaa gtcaaaattt tatgaataat ttagcttgga tagcgatgaa
    26461 aactacttca ccctttcgta gtactccctt gttcttgtgt ctatacggat tttatctcct
    26521 gtattgacaa ataagggaac gttgatcacc gaaccggttt caacggttgc gggtttcagg
    26581 gcagttgaag aagcggtgtc gcccttgagg cctggttcgg tgtaggtaac ctccatttcc
    26641 acaaacgggg gaagctcgca ggttagtatg ttctccttat cggcttctac catcatttca
    26701 acgtattgtc cttccttaag caggtcggag ttttcgatga ggtcgccgct caggctaacc
    26761 tgttcaaagg tttcctggtg catgaagtga aaaccaaggt catccttata gaggaactgg
    26821 tagggacgac gctcaacccg cacaacattt atttttaccc ctgaggtaaa tgtattttca
    26881 attaccttgc ctgtttcaag atttttaagc ttagttcgaa cgaatgctcc ccccttgccc
    26941 ggttttacgt gctgaaattg cactatggtg tataacttgc cgttaaactc aatgcatagc
    27001 ccgtttttga aatctgccgt tgttgccata aaatattttt aacaaattag ggtgcaaatg
    27061 taataattct ttaacttgtt gaaacaaatt tacaagtaag catttctcca tggagcattc
    27121 gggcacaaag aacaagcagc atgtttgggt ttaaataaaa acaacttagt gctctattgc
    27181 cggtgtaaga aaattatttc actttgggac tataggagga aaacggattt tttaaaccgg
    27241 aaaaactgta taaatcgcag tagaaggctc ctacaaaaat ctcgaagcgc actctaaccg
    27301 gcaccaggtt atcgtcgtcc gagagccaga ttgtcatatc gtcctgggtt ttaaaaactc
    27361 gtcctacctc tacaaccggc ataaatttca ggcatcgaaa ttctccaaga cttgttttaa
    27421 tggtttcccg acccttaaag cgaaccatta gcggaaattt ttcatcggta aagtaggtgt
    27481 caacgataaa gagctgatcc ttcacaggat ctgattttat caggatatta cgaaggtagt
    27541 agaacgacga gagtatatcc tgacatccct ttaccactac cacttctcct gttttggagc
    27601 tgttgactat gcttgagtcc ttacggctcc agtgatccca aacctgggtg ctgtagtgcc
    27661 ggtaacggtt ctcgtgaata ttcctaatcg agaagtatgg ccagtcggtt tcggggtcgg
    27721 tgtagctctc gtagatatcc tcaacgtagt aaaacttgtt ggccaggcca accgttttgc
    27781 cggccatgta aatatgattg gcaggctttc ccttgtagtc gcccttcctc acctcgagca
    27841 cagcctcgcc gccatgtata agcccgtagt aaattttgta gtttaacttt tcacccggtc
    27901 caaatgcctt ttgagccgaa gctaccggta tgcctagaat tagagtgcag gccagcagta
    27961 caacgaatcc ttggcgggat cgaagaagtt caaaattctt gttaatccgg aatgatcctg
    28021 ttttttggcc ttgtgggcac aaagtcctta aggtagtatg gctcaaaata tgcaatgtcc
    28081 tcaaacaatt catctctaaa tttcttttgc gccaaaggta gtaaatagcg agctgatggt
    28141 ttgaaatgtt caacaaatat tgcaccactg ttaccaatca cctgttgaca cttggatgct
    28201 ccgctcccga aaaatacgac agtatttttt tttagcaaat cgctgaaagt atccgtttcc
    28261 accaccaagg cttttacctc cgacagcatt tctcccttcc tgttaaaaag ttgggtgtaa
    28321 acctccattc gtcgggcatc tatcatgggg ctgagcagag agttgttgtt gatgctttcc
    28381 atgttgataa attctccaac cccggttacc attgcctcca ggctacccac agcgattaga
    28441 ggcttatgca gagcatagca cattcccttg gcgagcgaaa cacctatacg caggccggtg
    28501 taggagccag gacccatgct aacagcaatg gcatcaatat tccctaattc aaaacccgtt
    28561 tcccttatca gctcttcaat cagaacagga agcgccgagg catggatatt ggcatcggaa
    28621 acctctctga atccaataac gccgctctcg ctgcccagtg ctaccgagca ggttgttgag
    28681 ccggtttcca tgcaaagaat cgttgccatg ttggtgttgc aattgtgcga agctaattct
    28741 tcagggttgc tacagcaatt ttttgtctgc aaccctggca attcctattt tttcatttca
    28801 ggttgatgag ggcctgttgg cttaacgcaa aaacgttggg tttcaggggt gaaaagccca
    28861 ttaaacccta ccacctgttt gctgcgccat ccacaactta ctgccaagcc aagcctggca
    28921 gcagattggg tagataccgg gttaggaata acggtcaacc cgaatgctat ttctttgcaa
    28981 cctcagcttt caggagatca tttttggtct taaccttttt aaccagcgat ttgtccttta
    29041 gcttgcgtga gattgcgctg tagggagccg taaccacatc ctcatcaccc tttaatccct
    29101 caacaatttc gatgtactgg ttgttctgta ttccggtttt caccgtgata atcctggctg
    29161 tatcgccaga aacagcaaat acaatttctt taggtttgtt cagcttcgag ttggtttgtt
    29221 cgacgggggt tgacgaatcg tcaacctttt ccattccggt gatatccttc ttggcggttg
    29281 aatcgcttcg cactgtaacc gctagtatgg gtacggtgag aacgttgcta cgggtctcgg
    29341 taagaatttc aacggtggtt gacatgcccg ggcggaaagg gctaagccgg gaatcggtaa
    29401 ccaggtcttt atatgagtca tccaggagga atattctaac ctcaaagttg gtaacctggt
    29461 cggttgagac cccctgaaca tttgcagtgt tggcgatttg ggtaactatc cctttaaact
    29521 tgcgatccag gtatgcatca acctccacca gagcggtgtc gccaatattc acctttacaa
    29581 tatcattctc gttaacatca accctggcct ccatccggtt taggttggca atccgcatga
    29641 gttcggttcc tgccatttga atggttccca acacccgttc gcccagctct acgtttagct
    29701 tcgatacggt gcccgatatg ggtgagtata tggatgtttt gcggaggttt tcctcggctt
    29761 ccttcagcga tgcctgggcg ctttccacat tgaactcgga agccttgagt tccgatttgg
    29821 ccacctcgta gttggttttg gcggtttcaa attccgattc tgcaatggcc ttttgttcga
    29881 acagctgctt gctacggtta aaagccagct cgctctggct aagctgggct ttggcctgag
    29941 ctaaacgtgc attggcagag ttcaccgagg ctattgcacg atccctcatg gagatgtaag
    30001 tatcgggctt aattctgacc agcagcgttc ccttttcaac ccagttgccc tcctttaccg
    30061 gaagttctat tatctctcca gatacctcgg agctaatctt aatttcaact tcgggttgta
    30121 ttttcccgtt agcggtgatt gactcggtaa tagtcctttt ttggggtttc tctacataaa
    30181 cctcaatagc atcggttgag ccgaaccagc cctttttttt accaacaatg gctaaaacta
    30241 ttatgatgac cattgcgcca atcaggtatt tcagtaatcc ctttttcacg agagtatggt
    30301 ttagttaaac atcaaattat caaaagtatg tcgatatttt aaaacggtaa aaccttaatt
    30361 ggttgctgct gattcccaag ccaaaatgta catgttttat tacgaattaa acttaaaggc
    30421 tcaaaggaac acctttgtaa aagtcgagaa ttttggtttt aaaaacaagc tcgtatttag
    30481 cctgtagcag gtcggtttcg gccttggcca ggttgttacg ggctgttgta tagtctatgg
    30541 ctggcagcaa accaaggttg aacttattct cggtgtagcg gaacgattcc tcggaggcca
    30601 ttttactttt ctggctggcc ttatgttttt tctgtgcggc tatggcatcg gcgtatgcct
    30661 gctggatatc cttgtagaga atgtttttct cattttccag agttaggcgg gcgttatcga
    30721 gcgatatcct ggctgttgat accctatacc gggtgcttcc gccattaaat attggaattc
    30781 caaggctaag ccccaccgag gtgcttgcat tatcctttat ttgggtgaaa aatggatcgg
    30841 aagtgagcag tgggttgtcg ttgttcaggt agctctgagc tccggagctg tagctgccgc
    30901 taagagtaag gcgtgggtag tttgctcctt tggcaatttt taagcccatt tcggagctct
    30961 gcaccttata ctgtgaaccg agtatttggg gcatggtttg ttgggctacc gtaaaaattt
    31021 cctgtggatt ttggataaca gcaatgctgt caacctccga aatgtttggt ttggctattc
    31081 taaaattctc aacatcttta atctcaagta gctgggtaag gttaaggtat gccaggtcga
    31141 gctggttgcg ggaattgacc agctgcagct cgtcggaagc taggagagcc tctacctcgt
    31201 atagattgcc ttcgggaagg cttcctgcat ttaccagcag ggcggttcgt ttaacctgca
    31261 tttctgattg ctctacctga cgttgtgcca cttctaccag ctcctgctgg taaagaattt
    31321 gcagataggc cgaggcaacc gacagggcaa tattgttttt cacagtctcc acatcctgta
    31381 tgctggccaa caaatcgtag ttggactgct tcttggtgtt ggtaagctga aacccctcaa
    31441 acaagggcat gctgctacta aacgaaaaac ttgtagattg ggtattccta ctctccttct
    31501 ggttggtgat gtagttcgta actcgtccaa agctgtaggc gtggttggca ttcaggtttg
    31561 cgctagggta gatgctgagt ttcgactgta gcagcttatc cttactcatg tttacggaga
    31621 gttcacccat ttttatttgc aggttattct cgttggcata ctgaatgcat tgctccagtg
    31681 tccaaggatc ttgggcctga gccatacctg aaagcacctc cattgccagg gcaaaaagaa
    31741 ctatcttttt catccgttat cgatatttag ggtatttata gatgtttatg gtaattgtaa
    31801 ttcagctgta acttttttaa caaacaaaat aatcgtaggt tgaggtatga ccttttcata
    31861 catgttggtg ttggtattga catgtgtact ccatacgtat attcctgccg gtggtttggt
    31921 ttcattcagg tttcagaggt aatgttctgt cacggaaaga atttggccag ggtatgtttt
    31981 taagtttagc ttgttggata aacattacat catcgaaatt tgggtatttt gagtgtttaa
    32041 tgctcatttt ttaaccaaat tggccctagc aacaatctta catgtaaata ccactaagtt
    32101 gtaccagtta atgctgattt taacccgaat agctatacat gaagcatcaa aatattaaaa
    32161 ggtgcatgaa attaccctga agcgccacgt atggcaaaaa acgtgtaaaa tcaggattta
    32221 tcctttaccc cttccagctg ggcaaacaac aatattgaac atcccctcgg aacttcagct
    32281 caccattatg gttggtcaaa gttcctttct actgtttttt tgtgttgctt aacccggctg
    32341 caactaggat gctgctaaca gcctgtggtt taatttttga cgaaagtata gctaaaattt
    32401 gtaccgacca tttaaagaaa gctaaaactt taagtattca cacaatttat ttctcaaagt
    32461 tgaagtgaag ttgatgttta cctggctgaa attaatgttc aactatcatg tgtattgctt
    32521 gatggttgta gtaaggaagg tgctataaaa catattaatt ttgaactaaa ccaaaaaaaa
    32581 ctatttttgt caaatcaaaa aaaaaccaaa tcaataacct aataaattct aagaaatgtt
    32641 taaaaaccat cctaaaggtt tactggtctt agccctaacg aacatgggcg aaaggtttgg
    32701 atactacacc atgctggcta tccttacttt atacatgcaa gctaaatttg ggttaacagc
    32761 agcgtcaacc agcatgattt atggagcttt tctggcttct gtgtattttt tacccatttt
    32821 tggtggagtt ctggcggata aggtgctggg ctacgggaag accattttgc tgggtatttt
    32881 ggtcatgttt atagggtatt tacttttggc tattccactc gaaggcaatt ctgtggggct
    32941 gactatgatg attatctcgc tcgttttgat tgcgctgggt accggattct tcaaaggaaa
    33001 tttacaggcg ctggtaggta aactttacga cgacaagcgt tacgacacca accgggatat
    33061 tgccttttcc attttttaca tgttcatcaa tattggcgcc ttttttgccc caagtgctgc
    33121 taactacatc aacaatgccg tgatgaaatc gtccgatttc acctatgatg cctcaattcc
    33181 taaaaattat gttgttctga acgatgagaa gcaagcagta gataaaaaca cctttattgt
    33241 acaaaacctt acaccggccg agcagcagct ctccggtaag gagtttgatg cagtgcttac
    33301 taaagccaaa aagaagcaca aggtggtttt caccgaggat attgagcatg cgcttttccg
    33361 tctgaaggct gctgggctga accagtacac ccagtcgggc aaaattaccg atccggctat
    33421 gatgattagc aacaacgagt atgagctgct gcaaagccgt agccaggagg atatgctgaa
    33481 aaaagctgag gttactgcca aaattcaggc aatcagcatt accgatccgg cgctggaagg
    33541 aggtcccaat cccgacgatt ttgcccgtaa ctttggtaaa cactacgtta gcgaatcgct
    33601 atctacatcc tacaacctgg cctttggcgt ggcctgcatc agcctgatta tctctgtact
    33661 catattcctg cttttcaaga aatcgtggaa atcggttgat aaaacagtta agcaggaaat
    33721 agccgaatca aaggccaacg gcaaggaggt taaggtgatg tcgaaggagg aggttcggga
    33781 acgaaccgtg gcgttgctgc ttgtattttt cgtagtaatc tttttctgga tgtcgttcca
    33841 tcaaaacggg ttaacgctaa ccttctttgc gcgcgactat acccagagcg taatctcgcc
    33901 cggttggact atgctgttta acctgttccc attgctatcg cttattgttt tcctttatgg
    33961 aatttacctt tctacaatcg gattgctggg tggtaaaccc aataaaaaat caggactttt
    34021 actaacggtg ttgggtattg ccggattggc aggctactac tttacccgcg ttaagggaat
    34081 tgaggtaatg actaagatta ccccgcagat tttccagcag tttaacccca tgttcatcgt
    34141 catcctaacc ccgttaaccg ttgcgctgtt cagctattta ggtaagaaga aaaaagaacc
    34201 ttcggctccc cggaaaattg gtatcggtat gtttctggca gccattggtt ttaccattat
    34261 gctaatctgc tctttcggac tccctgcacc ctccgaaatt tcgggtgttt ccgatcagct
    34321 ggtgtctcca gcctggctca taagcaccta tttcgtgtta actgtagccg agctattcct
    34381 cagccccatg gggttatcct ttgtatcgaa agttgcaccg ccacagtaca gcggtcttat
    34441 gcagggtgga tggcttgcag ctaccgctat cggcaatttg ctggtagggg taatcggcac
    34501 tttctgggaa aaacttccac tggttggctt ctgggtaatt ctcattattt gctgcgtaat
    34561 ttcgggcata ttcatcttct ccgttatgaa gaggctcgaa aaagccacag ggaatgtata
    34621 gggggaggtt gaaaatactt tcatacccaa ctacccaaaa aattattatg gcctgaaatg
    34681 gtgaataccc atgaaatagt aaggctgccc gaagtgggca gccttactat ttctccaaat
    34741 tttttttttg aaaaataaaa agatgttggc tttcaaaaaa atagctacct ttgtgccgaa
    34801 tcgaaaatta agttacaata cgaatccttt acccacacct cagaacgtgc gtgagtgcaa
    34861 aagtttcctg tattgcctta aggctcgaaa aacaatcaat ttatcactta attcaattta
    34921 attttatgaa catttacgtg tcaggtctga gctacagaat taatgacgac gacctaaggc
    34981 aactttttga agagtatggc gagattacat cagccaaggt gatcaccgac agggaaactg
    35041 ggcgttctag aggttttggc tttgttgaaa tggccaacaa cgaagaggcc caaagggcta
    35101 ttgacgaatt aacgggagca gagtatgatg gaaaggtaat cagcgtttca ccggccaaac
    35161 caaaaactga caagccccgt aatgggggcg gttttaaccg tgagcgtcgg ggtggtttcg
    35221 accgtggtag ccgtggcggt agcggtaacc gtggtgaaag aagggattac taatccttac
    35281 gtagataact taaaaggctg tccttaaccg acagcctttt ttaatatata acttttccag
    35341 ggcgattgtt tttcctggta gttgttagcg taagcttggt ttgttgtctt caacacacga
    35401 tttagggttg ttgcgtcttc cccaaacgct ctatgctttc tgcaaaatac ttggtgttgc
    35461 cgcgccaggg aacacgcatg tagcgtttaa ggtaatgcac gttgaccctt tcgccagtga
    35521 gggcaacctc ctcaagctgt ttgattagct ccttttgctt gcgctcaact gagaaatcga
    35581 acgtttcggc aatggggctg gtgcttcgta atgctccaaa cgactcgatg ctgagcttcg
    35641 cctcgtaggt cttaaaaata acaccctttt ggctcaccct taaaattcgg ccggccataa
    35701 cacccttttc ataaactcca aaatagagga aagcccctgc acctatcagg agcagcacca
    35761 gcaccagtaa aatagtctta aaagtcttca tgctttttct cctagagttg tgttgaagta
    35821 aaagtgtgta gctgcatata aacccaatcc tacttgttgt tcataaaccc ttgctgctcg
    35881 atggtcttaa gaaatacttt cgaaaatagg aggttgtcgg ctcgggtgta gcgaatatcg
    35941 gtaaatattt gggccagggt ttccttacca ttttcctcaa gtagcttttt agtcagctgc
    36001 agcgattcct tgttcatgta aatttggtct atgtcgccct ccgagtagtc ctggtaaacc
    36061 tcatggtgaa ctactccggc taagggtaaa cgatcggtta gctccggcga ctcgtcgggg
    36121 tagcccatta caaccgtagt aatggggaca actccctttg gcagcttcag tacttcgacg
    36181 atgcggtcgg cggtgtaggt ggtggtaccc aggtagcaaa taccaaggcc gttctcctcg
    36241 gcggcaatgc atacattttg tgccaccagc aaagcgtcta ttgcaccggt gaaaaacgag
    36301 atgaagttat cgtatccggg ttcagccttt ctctgcccgc accatttgtt gaaccggttg
    36361 aaatcagcac aaaaggtcag cacaaccgga gcctctacaa ccatttgctg gttaaagtgg
    36421 cagggaagaa gctgttgttt tacttcctgg cttttggtta ctataatact gtaaacctgc
    36481 atgttgccag ttgttgaggc gcgaataccc gcctgaagga tttcgttaag cagcgattcg
    36541 tcaatcggag tgctcttata cctgcgtatg gaacgatggg aatggagtgc tttcattttg
    36601 gtatgagttt taatgaacca aaggaactaa ttttgaaaca aaatggttca aaaaaattca
    36661 tgttcttgga cattcatatt acacgagtcc gctgcggctc atctttttta tgccccagta
    36721 tcctcctcaa taatgtaggt gttcctgtcg gaggcgctgc gggacaccaa ctcgccgatg
    36781 aagccagcca ggaaaagttg aactcctata atggctatag ccagggcaat gtagaacagg
    36841 ggctggtcag ttactggtct gtacctcagc atgtgggttt gcgaccatat tttgttggcc
    36901 accagtacaa cggctatcac cccgccggca aaaaacatga gcgtgcccag tgttccgaaa
    36961 aagtgcatgg gtttcttgcc aaagcgggag acaaaggtaa ccgagagcag gtcgagaaag
    37021 ccgttgataa atcgcgacca cccaaatttt gacgagccgt acttgcgctc gtggtgctgc
    37081 acaatttttt cgccaatttt gcgaaaaccc gcctgcttgg caagaacggg gatgtagcgg
    37141 tgcatttcgc cgtacacctc cacgcttttc accacgttgt tgcggtatgc ctttaggccg
    37201 cagttcatgt cgtgcagctt tattccgctc accattctga cagtaaagtt gtacagcttg
    37261 ctgggaattc gcttggttag cagggggtcg tagcgtttct ttttccaacc cgagaccagg
    37321 tcaagctgct cctccttaat catcctgaaa agctcgggga tttcgtcggg gctgtcctga
    37381 agatcggcat ccatggtgat taccacgtca cccgatgctg cggtaaagcc aacctggagt
    37441 gcagccgatt taccgtagtt ccgcctgaac ctgattcccc taatggcggg gtactgtttt
    37501 cggagctcct gtataaccgc ccagctcctg tcgctgctgc catcatcaac cataatcacc
    37561 tcatggctca ggttgtgcag ggtcatcact cggttaatcc atgctgcaag ttcggacagc
    37621 gattcctcct cgttgtaaag cggaactaca atagatatgt ccattaattc agggctattc
    37681 ggaaagagtt tcaccctcct tgctctcgct gccgaaaagc ggggcttcct tcttaataaa
    37741 aatggaggta atcagcgaga agagggtacc cataaacaca aagccaaaaa cagtgcttaa
    37801 ggccatcatt atcggtgtca tcattttttt cgacatctcc aacgctgccg caatctgatc
    37861 ttcgctcagg ccattttcag cgtatgcctg ctccacggtt tcaagaacct tgtccatgta
    37921 gccggtatct ataaactttg tgagaataat gaagtagaag gcatagatga tggatgcgaa
    37981 aagcgaaacc agaactccag ttcccactgc tttcccatag gtaatgtaac catccagaat
    38041 gttatttctg tactttagct gagccattac tattccgcca atgatcagcg catagttcag
    38101 aatacccacc cacgtagggg ctttgtaaat attcatgatg taaagtacaa ggctcagaat
    38161 gatcaacgct agtcctacga ctaaacccca cgtcattgta acgtttaggt ttgatttttt
    38221 ttcttccata actaagtagt tttaggtttc agcaaatata tataaaaatg gttagaatga
    38281 aatgtaagga taaagggtaa agggtaatac aaggataaag gttaa
//
LOCUS       DAOSHM010000022        37188 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_874_length_37188_cov_20.134947, whole genome shotgun sequence.
ACCESSION   DAOSHM010000022 DAOSHM010000000
VERSION     DAOSHM010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 37188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 37188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37188
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_874_length_37188_cov_20.134947"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(174..467)
                     /locus_tag="PK839_05190"
     CDS             complement(174..467)
                     /locus_tag="PK839_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019129288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72168.1"
                     /translation="MKAVFIAYNQALSDRMDNALKVAGIKGFSKWTEMLGVGSNTGEP
                     HLGTHTWPSMNSGILTIVEDEKVDPLLNYIRQVNKIAEEQGIHAFVWDIERMV"
     gene            complement(550..3678)
                     /locus_tag="PK839_05195"
     CDS             complement(550..3678)
                     /locus_tag="PK839_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019129287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOG72169.1"
                     /translation="MSLYSTAVNKPVSTIMIFIAIIVLGIFSITYIPVDLMPDMDIPM
                     ISVITTYPGANAEDIEINVTKPMENQLNSLQNLKKLTSSSKDNVSYVACEFEYGINMD
                     EASNNVRDAVSLAENALPDDCEKPMILKFNSSMVPVLIYGIKADKSYAALEKIVDERI
                     VERLSRIDGVGTVTIMGKPIRIINVAVDPRKLDSYNLSLEQVGAAIGAENMMLPAGRI
                     EVGKMEFPVRTNGDFSSSDVVKDLVISNNAGKVVYLKDVATVKDTLKKILVDETIDGQ
                     KGVRLMVQKQSGANTVKIAKMVRSRMEEIKTELPSDVQIVKIMDSSEFIQGSINNLSE
                     TLIYAFIFVVLVVILSLGQWRSTFIILTTLPVSLISGFIYLYATGNSINIISLSAMSI
                     AIGMVVDDAIVVLENITKHIERGSTPREASIFATREVGLAVIASTLVIIAVFFPLTLV
                     GGLTGVFFRQLGWIVTITITVSVIAALSLTPMLSSRMLKSKSHYKHKHNSFVKIVNKF
                     WGFFDEIYGRTLKAILNHKTLTLTGAFIIFVGSLFLMKFIGTEFTPSQDNARISVYME
                     VPQGLRIDETKKLARDFEKKIKAKYPEIELISTSIGADEGGSLMSIFQKSASYIINYT
                     LRLSSIDNRTRTIFEIADSMRRDIAAYPQITTSYVDAGGTRNAGSSAMGMGGGNNVEV
                     KILGYDISQTNQIAEEIATTLKNEKGFKDVSISRDQDRMELQVIPDREKLAQLGLNTT
                     MLASAVRNRVYGFTATKYREEGTEYDVIVKYDDDNLTSISDLENITFKGPQGNLVRVS
                     DVAQVRQVYTTPNIDHENKTRIVKVSSSLTGIDLGKAMSIINAKLSTMTVPQNVLVEI
                     GGAAQDMMDTFKDLAQLFVLIMLLTYIVMATQFESLREPFIIMFSVPFAITGVLIALF
                     ITGTTLNMVSGIALVMLVGIAVKNSIVLVDFTNLLVDRGYSISRAVVEGGKSRLRPIL
                     MTSATTLLGMLPMALLKGEGSEIWRPLGIAIIGGLLFSMLISLIIVPVIYTLFANARI
                     KRERKSLQEANM"
     gene            complement(3717..4751)
                     /locus_tag="PK839_05200"
     CDS             complement(3717..4751)
                     /locus_tag="PK839_05200"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOG72170.1"
                     /translation="MKTARTLLILSFSALIASCTGNKTEVTGETKQAEVRVSTQPVEI
                     QKVEHQLRLPATVQAYKENHLAPATAGRVEKVLVDVGDRVRAGQVVAILDRTSYQQAK
                     IQFDKLSIDMARMDSLLKVGAVPQQQYDQVKMQYDIAKTNLQFLDDNTTLKSPIDGVI
                     TGKYINDGEMFAMTPVAGIGKAAIVSIMQLDRLKLLVGVSSSYLSKIELSSSVLITTE
                     VFPGEKFNGKIEKIYPTIDNLTKTFTIEVVVPNRSNMLRPGMFATAFITIGSGNSILI
                     PGYVIQKQSGTNERYVFTYSDGKAVRKVIEVGEVFDDKIEITSGLNPGDELIVVGQAN
                     LMDGQAVTKI"
     gene            complement(4778..6142)
                     /locus_tag="PK839_05205"
     CDS             complement(4778..6142)
                     /locus_tag="PK839_05205"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOG72171.1"
                     /translation="MRKTIFHVLYGLGVAMAVSLTPLSGYGQTDTLKLTVKDAVNMAI
                     QQNLMQKVSELELKKKEEKVGEYYAALYPNIKATGSYSRYLELPVIFIPEGTFGPGSG
                     GAMKIGYDNSYSGGFSATLPIFMMNIYQSIRMGKTDVEISAEKMRENQINLTASVKNT
                     FYNLLLLEQSYQVIEMSYQNAQANLENIQKLNASGMVSDYDAIRIKVQVDNLYPTLMQ
                     TKNTYNNLLNIFKVLLNVNDTIPVTLDHSELSTVTLEEIASPTEVWQQDNTTLRQLEL
                     SRKMIGIQEKMVRSSSLPSLVAMGNYQYQAQANDFKFNDYRWVPTSLVGLQLNIPIFS
                     GFAVRKQLNQVKISAQQLDMQMEFTRSNLSAQVQNSVNAINTAIKKVESAQGNVTLAQ
                     KGYAIAQTRYNTGQATLLELNDADNAMMQARLNLIQAQFEYLNARNEYEKITGQSALP
                     TPAN"
     gene            complement(6169..6792)
                     /locus_tag="PK839_05210"
     CDS             complement(6169..6792)
                     /locus_tag="PK839_05210"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOG72172.1"
                     /translation="MIVKEQIVNQAMLLFRQMGIRSVTMDMIAEQTGISKRTLYENFS
                     NKDELVEACVMQEIESRKEQAKRIIEGSSHIIETYILFMWDHINRLRGTSPLFIKDIQ
                     KLYPQSICKQTNEFNNRMRENMKQFIIMGIKDGVFRKEINPDIASILIFEQIKVLHVE
                     GGFETSPLSMFSPAEVFEHISINFIRGISTSKGLELVDFYYSKHKMV"
     gene            complement(7141..8580)
                     /locus_tag="PK839_05215"
     CDS             complement(7141..8580)
                     /locus_tag="PK839_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HOG72173.1"
                     /translation="MRLHVLSRYIGFVLLLNAAFILFSLLLSIHYHDSGFYPLLFTLL
                     LTTLWAVFPMIFVPKTQSLSNKEGYLVVVLSWIISCFIGMLPLLLYGGEFTVVKAFFE
                     SVSGYTTTGASIIKDVEALPKSLLFWRSSMHWIGGIGIVVFMLVILPSLGKAQLTLSR
                     MEISPLAQENFRFRTRKMLNVILYVYIGLTLCDIILLRIFGMNLFDAVTHAFATIATG
                     GFSTKNTSIAWFNSVPIEMVTMFFMFVSGIHFGLLYSTFTFKKSNVFRSPIVKYYFWV
                     TIAGIVAVAINLHGSTYDTWGECFRFASFQVISLSTTTGFANADSSIWPPLSIVIIIF
                     FTIQCACAGSTAGGLKVDRVVIFFKALKSQVLKIEHPQAVIPVRVGNRNISDEVVGSV
                     TLFIVFYILILLASTMLLAAMGVDLLTGFSASVTTMGNAGPGFGLVGSMSNFSTIPEP
                     GLVVLSLDMLLGRLEIFGFILLFLVRSWR"
     gene            complement(8584..9333)
                     /locus_tag="PK839_05220"
     CDS             complement(8584..9333)
                     /locus_tag="PK839_05220"
                     /inference="COORDINATES: protein motif:HMM:NF024140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCP four helix bundle domain-containing protein"
                     /protein_id="HOG72174.1"
                     /translation="MSIRLKILSGFIILASLLIISGLISIYELTKLGNSVNKLLRDNY
                     LSIDYARQMSHSLEKQNSAILLAVAGDKGSASDLFGISVKEFKENFEKAATNLTQPDE
                     KTLTDSISLVFNSYRQATESFIASSNASLNEYITTIRPMMETIQNSVDNLLLINQQAL
                     TETAAILKNSPYRTIMPGLIVIITSVVFSMLFYYMISYYFVKPVIGITKGINDFIKYK
                     RPFEVAVDTKDEVNELKEAVKNLTTLKNTPR"
     gene            9470..10039
                     /locus_tag="PK839_05225"
     CDS             9470..10039
                     /locus_tag="PK839_05225"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOG72175.1"
                     /translation="MDDKDLVGRVIESNDHKAFAQLVERYQKMVVTTCRGFVQSYADA
                     EDLAQDVFIELFESLPGFRNESKLSTWIYRIAVNKSLNFVRRRKREVLLNNLYFWNKD
                     SNSLALGQAVSPEGEQAMLDAERSKQLKLAINKLAENQRVALILSRYQELSYKEIAEV
                     MGVSVSSVESLLFRAKENLRKMLLKGKNI"
     gene            10086..10544
                     /locus_tag="PK839_05230"
     CDS             10086..10544
                     /locus_tag="PK839_05230"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HOG72176.1"
                     /translation="MKMNCKLAQQNMLNYLDGTIDKSIINAFEEHVGSCSSCRDELQL
                     LKPIYKQVEGDLGEYAPNPYFAAKVLAKLTEKREKPTTIFTQKSYILAVSLAAAAVAV
                     GLFVGIQFVSSGSSLAQPEIATILANDYSNFSDSNPYNFEVEEVDTSLNK"
     gene            10551..11099
                     /locus_tag="PK839_05235"
     CDS             10551..11099
                     /locus_tag="PK839_05235"
                     /inference="COORDINATES: protein motif:HMM:NF025173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HOG72177.1"
                     /translation="MNFLTKNRILVIVVVILSLLLLSILGGLGYGYYRSKQASKLPPP
                     ANRFEHQAAFISRSLNFSPIQNEQFIRLGEGFHFKFDSLATQIRLVSGEINNEITRAE
                     VDTLKLNMLIDRYANLQRQLKELSVRHLMEVRALCNPGQQQQFCRMARQINRRHQQMN
                     RNNSNDRMRGMPPRGHQNQNNF"
     gene            11124..11636
                     /locus_tag="PK839_05240"
     CDS             11124..11636
                     /locus_tag="PK839_05240"
                     /inference="COORDINATES: protein motif:HMM:NF025173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HOG72178.1"
                     /translation="MKRLSILLLGAIMAAGFTANAQNCGKGMGMGMGAPKMNPDSLQS
                     CNIPNLTPDQQKKIYDLRVAHIKAVTPLRNQLAEKYAHLKTLESAEKPDLNAINKTID
                     EIVSIKGQLMKSRAAHRADVSALLTDEQKVFFNAHQGMGNRMHQKGMGAGMHQGMGKN
                     KPMGSCQQMN"
     gene            11817..12677
                     /gene="speB"
                     /locus_tag="PK839_05245"
     CDS             11817..12677
                     /gene="speB"
                     /locus_tag="PK839_05245"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HOG72179.1"
                     /translation="MMIGYGGLPEAFTNYSTSKVAVLPVAYDGTSTWVKGADKGPYAL
                     LEASANMEVYDIETNSEVYRIGIFTDEIMEGDFSSPDQMVSAVRSRTLKLINDGKLVA
                     TIGGEHSVSIGAVQAHARTYDHLTVLQIDAHADMRSSYEGSPYNHACVMARVKELCPV
                     VQVGIRSMDSSEKTNIDPHRVFWAHQLVANNSWMDEAIDLITPNVYITFDLDGFDPSI
                     FPSTGTPEPGGLLWYPTLTFLRKVIESRNLVGFDIVELCPNPLDKSSDFLAAKLLYKI
                     LSYKYLNTVV"
     gene            12710..12907
                     /locus_tag="PK839_05250"
     CDS             12710..12907
                     /locus_tag="PK839_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72180.1"
                     /translation="MNKCKCVKVPPPEVGGKEFKLNAYYEFDYIPPVKDNPSFYKVFS
                     LEDNVSGSFSIKAFNEHFKKY"
     gene            complement(13096..13764)
                     /locus_tag="PK839_05255"
     CDS             complement(13096..13764)
                     /locus_tag="PK839_05255"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOG72181.1"
                     /translation="MYPILSKSPLFMGVSPVEIENMISQTPHVVKNFSKGQLVAQRND
                     EVKSLTILVEGSVKGEMADFSGKILKIEEMQAPKPIAHAFIFGEHNQYPVDVVALDDC
                     RILYIPKADFLALMQRNATVLINYLNSISNRTQFLSNRLWFLSFKTIKEKIAHYLLNL
                     SYGGANATLILPKSQQELAEFFGVTRPALARVFSDLEVEGIISIKRREVVIRDRNKLI
                     EIAS"
     gene            13944..14549
                     /locus_tag="PK839_05260"
     CDS             13944..14549
                     /locus_tag="PK839_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72182.1"
                     /translation="MNDHQTGKLDISFNTDLPEPLVFSTWKHHLNYIHDAILSIEVGS
                     ETTSLINEVAAGVGGTLVDLYIGELTPMVIAEEVIQQLVSFDALGYDSFSRWLGVSKS
                     GYQNMYLSDGSAWILRLGMVNRRYVHIHPARYSLNTVRCRSANLKFAIAYRLLYGMDD
                     SNFSIDKVNRTRETLGLPPAAEKIALSGALRILKQLSVKID"
     gene            15017..16687
                     /locus_tag="PK839_05265"
     CDS             15017..16687
                     /locus_tag="PK839_05265"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOG72183.1"
                     /translation="MRRNSFLYALTFSVICYACGSKERNVTSEQQVFRYNESKGISTL
                     DPAFARNLALIWPVTQLFNGLVQLSDSLAVEPCVAKQWHVSDDGLQYTFLLRDDVYFH
                     DSEVFPNGLGRRVTASDFVYSFSRLLNPKLASPGSWVLSMLDRNFAGFTNGCKALNDT
                     ILIIRLSKPFPAFLGLVSMPYCFVVPHEAIEHYGGEFGRRPVGTGPFYMKYWRDGEKL
                     VLRRNAHYFELDNEGKRLPYLESVVVTFVVDKQSEFIEFISGNIDMLSGVHASSKDEL
                     LTRTGNLNPKYREKVLMLTGPYLNTEYLGVLLDSIGLESLENSALHDLRVRKALGYGF
                     DRNAMMLYLRGNLGFPATRGIVPPAMPGFETSTEGFYYNPDKSRQLLAEAGYPNGNGL
                     PPITISTTDDYLDVCEFIQHQLGEVGFDIRIDVLPGAAYRDMMANSKLSLFRASWVAD
                     YADPENYLALFVSSNFSPLGPNYTHFSSSEYDQLYRKALAEPLYDNRLPYYRAMDSLV
                     IANAAVIPLYYDKVVRFISKDVEGITLNPMNLLELKRVRKSYSGLAPS"
     gene            16722..17579
                     /locus_tag="PK839_05270"
     CDS             16722..17579
                     /locus_tag="PK839_05270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72184.1"
                     /translation="MIVVADSGATKTDWRIIMEDFSSASFETVGLSPYFNTELDFNDA
                     LTHGFPKELNPNEVSKVFFYGAGCGTQERGSRVESYLHSFFPAAEIFAWSDALGAAHA
                     LLHNDQGIIVILGTGSNISYYDGAKLEAKTPSLGFILGDEGSGAHMGRLLLRDFFYGR
                     MDSVLGKRLSSIYDMDLAHVLNMIYDSPKPSAYLASFVPFLLNNVENPYISDLITRSF
                     EEMYQLHLSCYPNIRSIKIGVTGSVGCLFSSILDRVASQHGFKVYKYVRYPISSLVDY
                     HVNLYHSGR"
     gene            complement(17554..20034)
                     /locus_tag="PK839_05275"
     CDS             complement(17554..20034)
                     /locus_tag="PK839_05275"
                     /inference="COORDINATES: protein motif:HMM:NF017539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NACHT domain-containing protein"
                     /protein_id="HOG72185.1"
                     /translation="MVELLTQEVESRFGRKISCQKDCISLSISIMDTCREYLSPATLR
                     RLFGLLATNSNPSHVTLDILSRYAGYTGWDDFVSHKKSKIPEGIIRSADEDWEQAAEH
                     AKAVSSKTLNHIMHLSGIRFEETVHRRQVEERLDQFLNSNNPATAIIAPGGYGKSILL
                     AHWYQIHRDLKANMNDVFLLAPATMLDHYASSNTFIGHWLAQLLGQKSLESLLVKDLP
                     GRIVFIFDALDEVVSVGAKFNKILEALADLAEAFAQTNRFKLVISTRLSTWTQLKPYI
                     THPLLWYFSDSELLTHEGANIPPLSPDEVQQILDQTINLRFNQRLLIFELHPDLRQTL
                     SYPYFLQLFIQVYTPEKQNLLNNQHEILLEFLKSKIYFSTLPDEKVDILTEIIRLGNG
                     HRVLKEDIKRSYPIHLKLSGNYFTAYHELLSYGIITEEQVVESTGRYFKYVRITSPQL
                     TGLLIVQDIVNSTGGITEETFRQVEEKFAGTEFYPLVQGTLYYTGSRDRNEKVLVKFF
                     ELCPNAYTHGQLLREICATLRNSSYLLNVLMPEYTKTRLGRQLLVEQNIDLNHLNGYF
                     TTVLKHYLSNTTDISELFFAQTLLAFNGFITLNGMLAQPNHEATRNVKPPADVSPLVA
                     GAWFSNQLFAEIIMGNGNNTTPIVERATSYYRKFTSYAERAEFSELIVPTLLLLGQHP
                     AVENYLEDISSIAGSHYQVVNYFNRKLFEILSGKNHFSAEEDCQIQQIYQQLNPIRSF
                     PQVIAGEVGRAASYFHTQNLTMAHQCIRNAIELSGIAGYKLVEMKLMNGLARVLSEMR
                     EEKQAMLCREYAETLWKCSGFTSPNGIG"
     gene            20291..20419
                     /locus_tag="PK839_05280"
     CDS             20291..20419
                     /locus_tag="PK839_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72186.1"
                     /translation="MKKENSIREKITLKFKDDAGVFTIFFLVTLLFVYALIKALTL"
     gene            20448..21296
                     /locus_tag="PK839_05285"
     CDS             20448..21296
                     /locus_tag="PK839_05285"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HOG72187.1"
                     /translation="MLSQVWEQCHASVCSLHFINERGMVIDSLSGFKVNSSLITSEYA
                     FYINNAQKVEISFVEGDANTPTATMRIGYNEFINDLRIGYTNNQSDYAVFNINFSEFE
                     KIPGLTLCECRNFQIGKQVATLGFNFGENNLSIKSAVISSAFSSKNGIRCLELDGLTC
                     FGNSGSPVIDPDSMQVIAIISRRNTPAFKSYMELNRCIVSNIEELRKLEGKLKIDTID
                     PVQVLIANQNQIRLLASNLYKYTATGSSKAIMLDRILSYFNEQAITEQAQISSLEQKV
                     GYGLID"
     gene            21616..21852
                     /locus_tag="PK839_05290"
                     /pseudo
     CDS             21616..21852
                     /locus_tag="PK839_05290"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
     gene            21952..23385
                     /gene="aspA"
                     /locus_tag="PK839_05295"
     CDS             21952..23385
                     /gene="aspA"
                     /locus_tag="PK839_05295"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HOG72188.1"
                     /translation="MVDAASQYESGSTRVEHDLLGERDVPAEFYYGVQTLRALENFNI
                     SGVTLNFYPDLIDSLANVKQAAAEANFELGLIDEKVRDAIVAACKEITNGKLHNHFVV
                     DMIQGGAGTSTNMNANEVIANRALELMGYSKGEYQHCHPLNHVNLSQSTNDAYPTAVK
                     LAIVRSNAKLIKVLESLIESFRKKGQEFAHVIKMGRTQLQDAVPMTLGQEFEAYAVTL
                     GEEIERLTQNAKLFLEINMGATAIGTGINSDPEYPQKVLKHLRSITGLELVLAANLVE
                     ATQDTGAFVMYSSAVKRLAVKLSKICNDLRLLSSGPRTGLSEINLPPMQPGSSIMPGK
                     VNPVIPEVVNQIAFRVIGNDLTVTMAAEGGQLELNVFEPVIVHSIFESVEMLKNGMNT
                     LKYRCIDGITANEERCRSMVHNSIGLVTALNPYIGYEASTQLAKEALSSNRSVYQLVL
                     EKGLLTREQLDRILAPENMIKPGKMKL"
     gene            23551..24045
                     /gene="cdd"
                     /locus_tag="PK839_05300"
     CDS             23551..24045
                     /gene="cdd"
                     /locus_tag="PK839_05300"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HOG72189.1"
                     /translation="MSESNSFQFRYNRLRGKEELAPNERELVDRAVAALSSSYSPYSH
                     FRVGAAVLLENGEIITGSNQENGAYPSGLCAERVALFYAGARYPGIPVVSMAVVASSG
                     DGLTENPVTPCGACRQVMSEYRAVSGKPFAILMVGSNEIVRVEDVEQLLPFSFSLSNG
                     MKRK"
     gene            complement(24061..25668)
                     /locus_tag="PK839_05305"
     CDS             complement(24061..25668)
                     /locus_tag="PK839_05305"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOG72190.1"
                     /translation="MAYSIVAICGSERLSREIKSIVGIAEFDFDLHFVTDDTNIITNL
                     TQLNPDIVLFDVSYSEYAAPDLLTIIKNDIKTESVPILAIADFLQPERIQALFIAGAD
                     DFISQPLAAAELIQRTMIGIQRGRMLKKLKEQSRQFNSITDAISKAGNSMIVINHLGK
                     ITWVNEGFKRLYECPLDYFVSIFGDNLFDSSTNPVTREALDKCRKNGEYVVYESKWVT
                     PQGKEKFIQTSLTPIFDSLQNLSQIIAIETDISDLKAIEKDLEEKHEHLLTITENLES
                     VNAVLAEHQERIQKQALQLEEEKQKSENLLKNILPWEVAYALQKKGSYKPKKFKEVSV
                     MFTDFVGFSKISSNYGDIDEFLSVLGGFFEVFDEITSQRFIEKIKTIGDSYMCVGGLP
                     RVNRSHPFDTVLAAIEIQRFIHDKAVQAAMEGKPVWNLRIGIHTGSVIAGVIGKRKFA
                     YDIWGDTVNIASRMETASEPGKVNISQTTYSYIKDYFVCTSRGKVPAKNLGEIEMYFV
                     ERIKPEYSEDELGLIPNSSFRKIVASF"
     gene            complement(25877..26083)
                     /gene="xseB"
                     /locus_tag="PK839_05310"
     CDS             complement(25877..26083)
                     /gene="xseB"
                     /locus_tag="PK839_05310"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HOG72191.1"
                     /translation="MTKKPQRYIDAINEIEQIITEIENNELDVDDLTEKIRRASELLK
                     FCKTKLRATEEEIQKIISDFEEDS"
     gene            complement(26088..28973)
                     /locus_tag="PK839_05315"
     CDS             complement(26088..28973)
                     /locus_tag="PK839_05315"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG72192.1"
                     /translation="MKLVIQKWGVALLLTAYSLIAAQAGSPSSSKVDSLYQRVLAIAD
                     ENPQEASRLANLCRQESSKSGNSRDKALSLMANAQALIAMSLWNNASEKIDSASLFVQ
                     KLNDLNLEADLVDLRSNLEVKVSGSDAALKMLQKAYSQAIKSKRPDLGVKFLLLQAGL
                     YQQKGNLAEANRMREIALDQSSKAGCKKCQAECQQNIASSYWQQGRFNEALESYFKSL
                     ILFEDINDTIGMLAALKGVGISYRDLGQFEKSLTYLNRALKLSDSSNNYTEEAAILNI
                     IGSLYFKFNQVDDALKQYEQSLQIRKKLGLLRSAISTHSNMARAYLQKSMYKQAIEHL
                     NEALALQEQVVDPLAEASTLTEIGNLNLQQGNIAEALRHYLMALKLRQTYGQDEDIAK
                     SLTSIGLTYRRLGMYKSALKYLEQAREIIQDMKGSMNDAAYILQNLGHIYLDLNSPDK
                     AIATYKEALTLKEKSGDSIGAAKILKNIAQAQLKSSQPDQARTSISQALKLVTRTHST
                     SDIADLYNELGNVERQAKNPGMAIKHYHTAIENYKALNNNEGVALCLRKIGEIQVGQK
                     QLDEARKNINSSIEIGKQSDNKYLLSYGYLAQSELYRAQGLFDKALESYIKHIGIRDS
                     LESVKRNEANLEAQIDLELDQTKTEIKVMEAEVEALRQKTALDKAIIEKQRLFRNSLI
                     AIILLVVTAASIAFYSLIQKRRYAKLLEEKIEEIRLVNERLNQSESELRQTIKTKDKL
                     FSIIAHDLRSPFTALVGLSEVMATNLESLSPDEIREFSHHIHRSATEVLNLTENLLSW
                     ARSQTGKIELSPKPINLKDLINQVFSVAAIPAQEKKIKLRESIPANLTVFADFDTLQT
                     VIRNLVSNAIKFTPQGGSIGISAKNSSNSMVEIAITDTGVGIDPENQAKLFRVDGFTT
                     KGTNQENGTGLGLILCKEFVELNHGTIEVSSTPGLGTTFTISLPSNN"
     gene            complement(29079..31307)
                     /locus_tag="PK839_05320"
     CDS             complement(29079..31307)
                     /locus_tag="PK839_05320"
                     /inference="COORDINATES: protein motif:HMM:NF026288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing sensor
                     histidine kinase"
                     /protein_id="HOG72193.1"
                     /translation="MFKADNHPTKLWHLKKFRHILLLASVFSFHTGNILLAQNSSSVH
                     LLDSLLTEAKQTTGIRKITALDKISRYYWEKNPDSSLYYSNLALVEAQRDKSNEALGD
                     AYNSMGNAYSSKHENETAITYYLKSKDYREKSGDWTKVGHSLSNLGLTLRDLKRYSES
                     ISSYRQAAKYYSDAKNYLFEGISYLDIAQVYRQLNDLNRALENAILAINIFLEHDITQ
                     PLARCYNFIGNLHKDLKNFDLAEDYYLKAYEIWSKANNLSGISSALNNLGIIYDEKGN
                     YEKALENYNRSLDIAIQLNDSIGISTAYNNIGFIYVKLNQLERGINAYLKSMAISRQI
                     GEIESYLNTCNNVASVYLKAGMLDKCEEYLNIVLGNLKSITELAYIQETYQLLSELYA
                     KRGNYKKAFEFKSLQLAYNDSIYNQQQVRNILETQTRFETEAKEREIQLLKKDNEIKQ
                     LEFEKQRNFQNILTFITILLAIAIAVTIYISRIIRKKNLLLAEKNRALEEANKKMAET
                     EVNLRELNATKDKLFSIIAHDLKNPFNALLGFSDILERNYNFLSDEERIEYIGVINDS
                     TQSLYKLLDNLLQWTRTQTGTITYIPENFKLLPLVKQEITNVNLNSEKKKISITVNID
                     ENISVFADKNTTATIIRNLLSNAIKFTDLGGHIEIAARYKDQMVETSVSDSGIGINDN
                     DLDRIFMLDGSFTTKGTSNESGTGLGLLLCKEFVEKNNGKIWVESEKGKGSTFFFTLP
                     AK"
     gene            complement(31323..32735)
                     /gene="xseA"
                     /locus_tag="PK839_05325"
     CDS             complement(31323..32735)
                     /gene="xseA"
                     /locus_tag="PK839_05325"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HOG72194.1"
                     /translation="MSNGSTRLTLLELNLLVKETLAKSLAGSYWVVGEISEITEHPSG
                     HCYMELVQKGDDTDTIRAKARANIWGYTYRMLKPYFESTTNRPLSRGMKILVNAQVVF
                     HEYYGYSLTVLDIEPEYTLGDIELKRREVIARLISDGVMNMNKELELDMLPQRVAVIS
                     SANAAGFQDFVKQLTHNPHGYAFTYELFPALMQGDEAELSIINALGKIYDKLACFDLV
                     TIVRGGGAQTDLSCFDSYALASNIAQFPIPVITGIGHDRDVTVADMVSHTSLKTPTAV
                     AEFLIDKFRDAEAVANNLAGSVSQLAGKLLADMKESLTSNQVELQAILNQKVAQNKLY
                     LQQLTSSLPHMAKLVQVLHADELKSLNIRLSIPISRIKANYVKLNAYAGLLKSMPHKL
                     LQNEKIKIEHHFSTLKNLHPDSILHRGFTITYSNGKIVKNLNNLNVNDVITTQFWNGS
                     IESNITKIKASKKLLANNKK"
     gene            complement(32741..34372)
                     /locus_tag="PK839_05330"
     CDS             complement(32741..34372)
                     /locus_tag="PK839_05330"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOG72195.1"
                     /translation="MKYSFRILLIIQLVGLSSAAKPQSKYFIGFTDKANSPYTISNPE
                     QFLSIRSIERRTRQNLALTESDLPVNPSYLDTLANMGITIHFPLRWMNGVVATISTPD
                     QLTQIQNIPFVSGIKEIYTPTKEHKSSKFTIEEPQSQPFSKPDKDAPNYGISATQVNM
                     LNGQTLHSNGFLGDGFEIAVLDAGFYNVDSHPVFDSLWMNKRILGTKDFVNPTSNIYN
                     EHAHGMMVLSIMGGYANGQLVGTAPHARYWLIRTEDASSEQLVEEYNWAAGAEFADSA
                     GADIINTSLGYTTFDVPSQNHTYSNLDGQTTVITRAANMAASKGMLVVVSAGNDGTSP
                     WYYISAPADSPKVLTIGAVDGNRNKAAFSSFGPTADERVKPDLCAMGVGTIIATTDGL
                     VGSGNGTSFSAPLISGMMACLWQAKPNLTVNELMESVVSASSSFNSPNNEIGYGIPDF
                     ALAMPVGTYSYKPNIRIIPNPFSSQVQIYLPLEIDEAVSCQLLTPSGLTLYMCRQITE
                     NGVLRINIPESLPKGLYIFHIAAGNQAFNLKAIKD"
     gene            complement(34384..35181)
                     /locus_tag="PK839_05335"
     CDS             complement(34384..35181)
                     /locus_tag="PK839_05335"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HOG72196.1"
                     /translation="MMKKIHILVIAALLPSFIQAQSDTVFNRTDEQGLKQGLWRKYYS
                     TGVKAYEGWFKDNKPTGEFKRFHENGSLKVILSYRQDSTAWAVFFSPKGNKIAEGKFV
                     GQQKDSLWRYYNPDGTINYDEYYQNGLKNGRCRQYYPDGKLLESAPFTNGKLHGAVIQ
                     FFPEGLNKSVVNFNNGIQDGDLKVFYPSGRKRLEGKYANGLKEGEWIFYGENGKITQR
                     IRYVKGVPENYNELLERETHLLDSLMQNAGRIKEPDFDEMSYGSNYP"
     gene            complement(35863..>37188)
                     /locus_tag="PK839_05340"
     CDS             complement(35863..>37188)
                     /locus_tag="PK839_05340"
                     /inference="COORDINATES: protein motif:HMM:NF016232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG72197.1"
                     /translation="ESQNIEYKSSWHNDYLKWICGFANAQGGKIYIGMNDAATVIGVE
                     NQKTLMEEIPNKIKNNMGITAEVNLLHEGDKDYIEIVVQPYSVPISLRGRYYYRSGSV
                     MQELTGAALNDFLLKRAGYTWDDVIDERATFADIDEPTIKKYLGKAEEVGRLPDIDGL
                     STPELLEKLRLAQDGKLKRAAIVLFGKDPGKFYPNTFVKIGKFEDDDFTIRFQEVEEG
                     NIIQVLDKVLRTLDYKFLIRNISFEGMNRVETLEYPITALREMLLNALIHRNYMGAPT
                     QIRVYDKKLSVWNDGGLPPTITLSQLKENHSSHPRNPILAEACFKGGYIDSWGSGIMK
                     IINSCKIDGLPVPDLKEKEGGFIVTLFKDRFSKEQLEKLGLNDRQMKAVEYVKTKGKI
                     TNAVYQEINGIGKSVTIDELRDLVEQQIFVRIGETGRGTYYELANKNDR"
BASE COUNT         9528 a         7876 c         8652 g        11132 t
ORIGIN      
        1 cggtagtatc ccaaaaagtg tgtaaagctt aggtttttca tctttttaaa agtttacact
       61 tcaaaattat gaaagcctga ctttattgct tcccgaatac tatggtttaa ttttaataca
      121 aagggctacc ccgttcgatg tagcccttgc tttcttgccg gtagatggag tttttacacc
      181 atcctttcga tgtcccaaac aaaggcgtgt attccctgct cttcggcaat cttgttgact
      241 tgcctgatgt agttgagcaa agggtcaact ttctcatcct caacaatggt gagaattcca
      301 gaattcattg atggccaggt gtgggttccc agatggggtt caccggtatt gctgcccacc
      361 ccaagcatct ctgtccattt gctgaatccc ttaattccgg ccaccttgag ggcgttgtcc
      421 attctgtcgc taagtgcctg gttgtaggct ataaaaactg ctttcattat tttcggtttt
      481 atgttcagtt ttaggaggag tgccattggg aatagccctt ttggctactc ctcccgaagg
      541 tgtttacaat tacatgttag cttcctgaag tgattttcgt tccctcttaa ttctggcatt
      601 tgcaaacagg gtgtagataa ccggaacgat aatcagggag atgagcatgg agaataaaag
      661 tccaccaata attgcaatac ccagcggtct ccaaatttcc gatccttctc ccttaagcag
      721 tgccattggg agcataccca gaagcgttgt agccgaggtc atcagaattg ggcgcaacct
      781 ggattttcca ccttccacaa ccgcacggct gatggagtaa cccctgtcaa ccagcaggtt
      841 ggtgaagtca accagcacaa tcgagttctt aacggctata cccacaagca ttacgagtgc
      901 aattccggaa accatgttaa gggtggtgcc ggtgataaag agtgcaatca gcacaccggt
      961 tatggcaaac ggaacggaga acatgataat aaagggttcg cgtagcgatt caaactgggt
     1021 ggccatcaca atgtaggtta gcagcatgat tagcacaaac agctgggcta aatctttgaa
     1081 tgtatccatc atgtcctgtg ctgctccgcc aatttcaacc agcacatttt ggggaacggt
     1141 catggtggaa agtttggcgt ttataatgct catagctttc cccaggtcaa ttccggtaag
     1201 tgagctggaa acctttacaa ttctcgtctt gttctcgtgg tcaatgttgg gggtagtgta
     1261 aacctgtcta acttgcgcca catcgcttac ccttaccagg tttccctgag gtcccttaaa
     1321 ggtaatgttt tccaggtcgc ttattgaggt taggttatca tcgtcgtact taacaattac
     1381 atcgtactct gttccctcct cacggtattt ggttgcggta aaaccataaa ccctgttacg
     1441 gacggcgctg gccaacattg tagtgttcag cccaagctgg gcaagttttt ctcgatcggg
     1501 aataacctga agctccatac gatcctggtc acggcttatg gaaacatcct tgaacccctt
     1561 ctcattttta agcgtagtgg caatttcttc ggcaatctgg ttggtttggc taatgtcgta
     1621 gccaagtatt ttaacctcaa cattattgcc accgcccata cccattgctg agctacctgc
     1681 gtttcgggtg ccacctgcat caacgtatga ggtggtaatc tgtgggtagg cggcaatatc
     1741 acgcctcatg ctatcggcaa tttcaaagat tgttcgggtt cggttatcaa tgctgctcaa
     1801 cctcaacgta tagttgatga tgtacgaggc tgatttctgg aagatgctca tcagcgatcc
     1861 cccctcgtcg gcacctatgc tggttgaaat aagctcaatt tcaggatatt ttgcctttat
     1921 ctttttctca aaatcgcgtg ccagcttctt tgtttcgtca attctcaacc cctggggaac
     1981 ctccatgtaa acgcttattc gggcgttatc ctgtgagggg gtgaactcgg tgccgatgaa
     2041 cttcattagg aatagactac ccacaaatat tatgaaagca ccagtcaggg ttagagtctt
     2101 gtggttcaaa atagctttca gggtgcgacc gtatatctcg tcaaagaatc cccaaaattt
     2161 gtttacaatt ttgacaaagc tgttgtgttt gtgcttgtag tggcttttgc tcttaagcat
     2221 tcttgacgag agcattgggg taagggaaag cgcagcgatt accgaaacgg tgatggtaat
     2281 agttacaatc catcccagct gtctaaagaa aacacctgta agcccgccaa ctagcgtcag
     2341 agggaagaaa accgctataa taaccagggt ggaggcgata acggcaagtc caacttcacg
     2401 tgttgcaaat atagatgcct ccctcggtgt acttccccgt tcaatatgct tggtaatgtt
     2461 ttcaagcacc acaatggcgt cgtccactac catcccaatt gcaatagaca tggctgatag
     2521 tgagattatg ttgatggagt tgccggtggc gtaaaggtat atgaaaccgc ttatcagcga
     2581 aacggggagt gtggtcagaa taataaaggt tgagcgccac tgcccaagcg acaggattac
     2641 aacgagaact acaaatataa aggcataaat tagggtttcg gaaaggttgt taatagagcc
     2701 ctggatgaat tcggatgagt ccatgatttt aactatctgg acgtcagatg gcagttccgt
     2761 tttaatctcc tccatgcggc tgcgaaccat cttggcaatt tttaccgtgt tggcgccgct
     2821 ttgcttctga accattagcc tgacaccctt ctggccgtct attgtttcgt caaccagaat
     2881 tttctttagg gtatccttaa cagtggccac atctttaagg taaacaacct ttccggcatt
     2941 gttggagata accaggtcct ttaccacatc gctgctgctg aaatcgccat tggtcctaac
     3001 aggaaactcc atttttccaa cttcaatacg tccggcagga agcatcatgt tttcggctcc
     3061 aatggctgct ccaacctgtt cgaggctgag gttgtacgag tcgagcttgc gggggtcaac
     3121 ggcaacattg ataattctta tgggcttacc cataatggtt actgttccca ccccgtcaat
     3181 tcggcttagc cgttcaacta ttctttcgtc aactattttc tccagcgctg cgtagctctt
     3241 gtcggcttta atcccgtaga tgagcaccgg caccatggac gagttgaatt tcagaatcat
     3301 gggtttttca caatcgtcag gaagcgcatt ttcggcaagg ctgacagcat ccctaacgtt
     3361 gttgctggct tcatccatat ttatgccata ctcgaactca caggcaacat acgacacgtt
     3421 gtccttgctc gacgaggtta gcttcttgag attctggaga gagttaagct ggttctccat
     3481 gggtttggta acgttgatct cgatatcttc ggcgttagcg ccagggtagg tggtgataac
     3541 gcttatcatc gggatgtcca tatcgggcat caagtcaacg ggaatgtagg tgatagagaa
     3601 tattcccagc actataatgg caataaaaat cattatagta ctaaccggtt tattaactgc
     3661 tgtgctatat aaactcataa tgcgtgtttt aattagctga taaaaaaagg ttggtactaa
     3721 atttttgtta ccgcttgtcc atccatcagg ttggcctgtc caactactat gagctcatcg
     3781 cctgggttaa gcccgctggt aatctcaatt ttatcgtcga aaacttcacc cacttcaata
     3841 actttccgaa cagcttttcc atctgagtag gtgaatacgt agcgctcgtt ggttcccgat
     3901 tgtttctgta taacatatcc gggaattagt atagagttgc cgcttccaat ggtgatgaat
     3961 gccgtggcaa acatacccgg acggagcatg ttcgaacggt tgggtacaac cacctcaatg
     4021 gtaaaagttt tggtgaggtt atcaatggtg gggtatatct tttcaatttt accgttgaac
     4081 ttttcgccag ggaaaacctc ggtggtaatc agtaccgagg agcttagctc tatcttgctt
     4141 aggtagcttg acgaaacgcc aaccagcagc ttaagcctgt cgagctgcat gatggaaaca
     4201 atagctgcct ttcctatgcc ggctaccgga gtcatggcaa acatttcccc gtcgttaatg
     4261 tactttccgg tgataacgcc gtctatgggt gatttcaggg tggtgttatc gtcaagaaat
     4321 tgcaggttgg ttttggcgat gtcgtactgc atcttaacct gatcgtactg ctgttgagga
     4381 actgctccaa ccttcagaag ggagtccatg cgggccatat caatgctaag cttgtcgaac
     4441 tggattttag cctgctggta gcttgtccta tcgagaatgg ccactacctg tccggctctt
     4501 accctatcac ctacgtcaac cagaactttt tccacccttc cggcggtggc gggtgccaga
     4561 tgattttcct tataggcttg aacggttgcc gggagtcgca gctggtgttc taccttctga
     4621 atttcaacag gttgtgttga tactcttacc tcggcctgtt ttgtttcacc ggtaacctcg
     4681 gttttgtttc cggtgcacga ggctataagc gctgaaaatg aaagtattag taatgttctt
     4741 gctgttttca tagtctgaag tttatttttt ttgttgatta gttagctggt gttggtaaag
     4801 cggattgtcc tgtaattttt tcgtactcgt ttcgggcatt tagatactcg aattgagcct
     4861 gtataaggtt gagcctagcc tgcatcatgg cattatcggc gtcgttgagt tcaagcaggg
     4921 ttgcctgtcc ggtgttgtaa cgggtttgag ctatagcgta acccttttgg gcaagggtaa
     4981 cattcccctg agccgattct acttttttta tggcagtgtt aattgcatta acggagtttt
     5041 gaacctgggc ggaaaggttg ctgcgggtaa actccatctg catgtcgagc tgctgggcgc
     5101 taattttaac ctgatttagc tgcttgcgaa cagcaaaccc gctaaaaatg gggatattaa
     5161 gctgaagtcc aacaagcgat gtgggtaccc agcgataatc gttaaactta aaatcgttgg
     5221 cctgggcctg gtactggtag ttccccatag ccaccagcga gggcaggctg ctgcttctta
     5281 ccattttttc ctgaatgcca atcattttac gtgaaagctc cagctgccta agtgtggtgt
     5341 tgtcctgctg ccaaacttcc gttggggaag caatttcctc aagggtaacc gtgcttagct
     5401 cgctatggtc gagggttaca gggatggtgt cgttaacgtt gaggagcacc ttaaaaatgt
     5461 taagcaggtt gttataggtg ttttttgtct gcataagggt gggatatagg ttgtcaacct
     5521 gaactttgat tcgaatggca tcgtagtcgg aaaccattcc gctggcattt agtttttgaa
     5581 tattttccag gttggcctgg gcattttggt agctcatttc gataacctgg tagctttgct
     5641 ccagtagcag gaggttgtag aacgtatttt ttacactagc tgtcaggttg atctggtttt
     5701 cccgcatttt ttcagcagag atttccacat cggttttccc catccggatg gactggtata
     5761 tattcatcat gaaaatagga agggtggccg aaaaacctcc cgagtagctg ttgtcatagc
     5821 caattttcat ggctccgccg cttccagggc caaacgtacc ctcaggaata aaaattacag
     5881 gtagttcaag gtacctgctg tagctgccgg tagcttttat gttggggtaa agggcggcat
     5941 agtactcgcc aactttttcc tcctttttct ttagctccag ctccgacacc ttttgcatca
     6001 gattttgctg gatggccatg tttacagcat cctttacggt gagcttaagg gtatcggttt
     6061 gcccataccc ggacaatgga gtgagcgata ctgccattgc cactcctaat ccatataaca
     6121 cgtgaaagat tgtttttcgc ataaggtaca tattaggatt tttaagtgtt agaccatttt
     6181 atgttttgag tagtaaaaat ctaccagttc aagccccttg ctggttgaga taccccgaat
     6241 aaaatttata gaaatatgtt cgaaaacttc ggcaggagaa aacatggaaa gtggcgatgt
     6301 ttcaaatccg ccttcaacat gcagcacttt tatctgttca aaaatcagga tggaggcaat
     6361 gtcggggttt atctccttgc ggaacacccc atccttaatc cccatgatga tgaactgctt
     6421 catgttctcc ctcattcggt tgttgaattc gtttgtctgc ttgcagatgc tttgagggta
     6481 aagtttttgt atgtccttaa taaacaaggg gcttgttcca cgcagccggt tgatatggtc
     6541 ccacataaag aggatgtagg tttcaatgat gtgtgaactt ccctctataa ttcgcttggc
     6601 ttgttccttt cggctttcaa tttcctgcat cacgcaggct tcaaccagct cgtccttgtt
     6661 cgagaaattc tcgtaaagcg tacgcttcga tattcctgtt tgctcggcaa tcatatccat
     6721 ggttacactt cttatgccca tttgccggaa tagaagcata gcctggttta ctatttgttc
     6781 ctttactatc atttttcggt tttaccctcc tttaatttga ccgcaatgtt attacaaaaa
     6841 actttttaaa caataaaagt ttttttaata tttgttaaat attctttaat tttttgtagc
     6901 agctcctcta tgctttatgc ggaattacac acacttcctt taaatataaa gtatttgttc
     6961 gaacgggtgg aaggcaaagc tacatcaaca agccgatagg cgagaaaagt tgtaagggtg
     7021 ttagttctct tgtaagctac acaagggcga ataactactt cttaaagtgc tgctagaatc
     7081 ttgctatcat gcaccgaaaa tgtgtagaat gccattatag cttagaaggt aaatatgagg
     7141 ctaccgccac gacctaacca gaaaaagcag gatgaagccg aaaatttcga gccttcccag
     7201 cagcatgtca agtgaaagaa ccacaaggcc tggttcgggg atggtagaaa agttggacat
     7261 tgatccaacc agtccaaaac ctgggccagc attacccatg gtggttaccg aagcagaaaa
     7321 ccctgtaagc aggtcaaccc ccattgcagc cagcagcatg gtgctcgcca gcaaaatcag
     7381 gatgtagaaa acaataaaca gggttactga gccaaccacc tcgtcagaaa tatttctgtt
     7441 gcctacccta acggggataa cagcctgggg gtgctctatt tttagcactt ggcttttgag
     7501 cgctttaaag aaaataacaa ccctgtcaac ctttaaacct ccggctgttg aacctgcgca
     7561 ggcgcattga atggtaaaaa atatgatgat gacaatagat aggggtggcc aaatagatga
     7621 atcggcattg gcaaaacccg tggtggtgga gagggaaatt acctgaaaag aggcaaacct
     7681 aaagcattcg ccccatgtat cgtaggttga tccatgaagg ttgatggcaa ctgcaactat
     7741 tccggctatg gttacccaaa agtagtattt aacaatgggg gagcggaaaa cattgctttt
     7801 cttaaaggta aaagttgagt atagaagccc aaaatgaata cccgaaacaa acatgaagaa
     7861 catggttacc atctcaatag gcacgctgtt aaaccatgca atgctggtgt tttttgttga
     7921 aaacccccct gtggcaatgg tagcaaaggc atgggttacc gcatcaaaca ggttcatgcc
     7981 aaaaattctt aacaggatga tatcgcaaag ggttagcccg atgtacacgt agaggatgac
     8041 gtttagcatc ttgcgtgttc ggaatctgaa gttttcctgg gcaagtggtg agatttccat
     8101 tctggatagg gtcagctggg ctttacccag cgaggggaga ataaccagca tgaaaacaac
     8161 aatgccaata cctccaatcc agtgcatgga ggagcgccag aatagcaggc ttttgggcag
     8221 ggcttccaca tcttttatga tggaagctcc ggttgtggtg tagcccgaaa cgctctcgaa
     8281 gaatgccttc accaccgtaa attcaccacc gtaaagtaga agcggaagca ttccaatgaa
     8341 acatgatata atccagctga gcacaacaac caggtagcct tccttgttgg aaaggctctg
     8401 tgtctttgga acaaatatca tggggaaaac cgcccatagg gtggttagca gcagggtgaa
     8461 gagtagcggg taaaaaccac tatcgtggta atggatggat agtagaagtg aaaacaggat
     8521 gaaagctgcg tttagcagga gaacaaatcc aatgtagcgg gaaagaacat gaagtctcat
     8581 ctattacctg ggtgtatttt ttagggtggt aagattcttg acagcttcct tcagctcgtt
     8641 tacctcatcc ttggtatcaa cagcaacctc aaatggacgt ttatacttta tgaaatcatt
     8701 aatccccttc gttatcccga ttactggctt aacaaagtag taggaaatca tgtagtaaaa
     8761 tagcatggag aacactacgc tggtgataat cactatcaat ccaggcatga ttgtccggta
     8821 agggctattt ttaagaatgg cagctgtttc ggttagggct tgctggttta ttagcaggag
     8881 gttgtccacg ctgttctgta tggtttccat cattggtctt atagttgtaa tgtattcgtt
     8941 cagtgaggcg ttagacgatg caataaatga ttctgtagcc tgcctgtagc tgttaaagac
     9001 tagcgaaata gagtcggtaa gcgttttttc atcgggttgg gttaggttgg ttgcagcctt
     9061 ttcaaaattt tccttaaatt ccttcacaga aatgccaaaa aggtctgaag cgctaccctt
     9121 atcgcctgca acggcaagca gaatggcact attttgtttt tccaggctat gcgacatttg
     9181 ccgggcatag tcaatgctta agtagttatc ccttaaaagt ttatttaccg agttgcctag
     9241 cttggttagc tcataaatcg aaattagccc tgaaataatc agcagcgaag ccaggattat
     9301 aaaccccgaa agtattttta ggcggatact catttttttt tacaaatgta taacgtaatg
     9361 gtggagttta atacgattgg gattgttaaa ttcgattttt tagtaaaagt agattgcacc
     9421 gcaagaaatg gcaggttgag ttgtcaaaaa tacattacat tcgtagctga tggacgacaa
     9481 ggatttggta ggcagggtta ttgagagtaa cgaccacaag gcttttgccc agctggtgga
     9541 gcggtaccaa aaaatggtgg ttacaacctg ccgggggttt gttcaatcct acgccgatgc
     9601 cgaggattta gcacaggatg ttttcatcga gctctttgag tccctacccg gctttcgtaa
     9661 cgagtcgaaa ctttcaacct ggatataccg aatagcggtt aataaatcgt taaattttgt
     9721 tcgccggcgt aaacgcgagg tgctgctgaa caacctgtat ttttggaata aggatagcaa
     9781 ctcgttggct cttggacaag ccgtttcccc ggagggagag caggcaatgc tggatgctga
     9841 aagaagcaag cagctgaagc tggccattaa caagctggcc gaaaatcaac gggtagccct
     9901 aattctaagc aggtatcagg aattgtcgta taaggaaatt gccgaagtta tgggagtttc
     9961 tgtatcttcg gttgaatcgt tactgttcag ggctaaagag aaccttcgaa aaatgttgct
    10021 caaggggaaa aatatttgaa aatcatcgca agaaatcata ctacaacttg tctaattata
    10081 aaattatgaa aatgaactgt aagctggcac agcaaaatat gctgaactac cttgatggca
    10141 caattgacaa gagcattatc aacgccttcg aggagcatgt tggttcatgc agcagctgcc
    10201 gggatgagct acagctacta aaaccaatat acaagcaagt ggaaggagat ttgggcgagt
    10261 atgctccaaa tccttacttt gccgcaaaag ttttggcaaa gctgactgaa aagcgggaga
    10321 aaccaacgac catttttaca caaaagagct acatactggc agtatcgcta gccgctgctg
    10381 ctgtggctgt tggactattt gtcgggatac aatttgttag ctcagggtca tcgttggcac
    10441 agcctgagat tgctacaatt cttgccaacg attatagtaa tttctcggat agcaatccgt
    10501 acaattttga ggttgaagag gttgatacct cactaaataa gtaagccacc atgaactttt
    10561 taacgaaaaa cagaatactg gtaattgtcg tagtcattct ttcactgctg ctcctttcaa
    10621 ttctgggagg tttggggtat ggctactaca ggagcaaaca agcctcaaag ctacctccgc
    10681 ccgccaacag gtttgagcat caggcagcat ttatttcccg atcgctaaat ttttcaccca
    10741 tacaaaacga gcagttcatc aggcttgggg aaggatttca tttcaagttt gactcgctgg
    10801 caacacagat tcggctggtt tcgggtgaaa ttaataacga aattacccgg gccgaagttg
    10861 atacccttaa gctgaatatg ctcattgaca ggtatgccaa cctgcaacga cagcttaagg
    10921 agctatcggt aagacatcta atggaagtaa gggcactttg caaccctggt caacaacaac
    10981 agttctgccg gatggcacgt caaatcaacc ggcgtcacca acaaatgaac cgaaataaca
    11041 gtaatgatcg catgcgtggt atgcccccca ggggccacca aaatcagaat aatttctaaa
    11101 ttttaactta aacttgcaaa actatgaaac gattgagtat tttactgctt ggtgccatta
    11161 tggcagccgg atttacagcc aatgcgcaga attgcggtaa gggtatgggc atgggaatgg
    11221 gagcacccaa aatgaatcct gatagcctac aaagctgcaa catccctaac cttactcccg
    11281 atcaacaaaa aaagatttac gatcttcggg tagcccacat caaagccgtt accccacttc
    11341 gtaatcagct ggctgagaag tatgcccatc tgaaaacact ggaatcggcc gaaaagcctg
    11401 atttgaatgc catcaacaaa actattgatg aaattgttag cattaaaggg caacttatga
    11461 agagtcgtgc tgctcatagg gccgatgttt cagcgttact taccgatgag caaaaagttt
    11521 tctttaacgc ccatcagggg atgggtaata gaatgcatca aaagggcatg ggtgctggga
    11581 tgcatcaggg aatgggaaag aataaaccca tgggttcatg ccaacaaatg aactaagcca
    11641 attaaaaata agaattgaag gtaaaatgct ggcttctccc aagcggggag gccagttttt
    11701 tttgtatgat gaaaacccta gctgtttcgg gcagttggtt attggccatt atcatgaaaa
    11761 attcaatgtt aaattctcca aaaatttagt aattttgcaa agttttttaa ggttttatga
    11821 tgattggata cggcggacta ccagaggcat ttacaaacta cagtacatca aaggtagccg
    11881 ttctacctgt ggcttacgat ggcaccagca catgggtaaa gggcgccgac aagggaccct
    11941 atgcacttct cgaagcttcc gcaaacatgg aggtttacga cattgaaacc aactccgagg
    12001 tatataggat aggcattttt acagatgaaa tcatggaggg cgatttcagc agccccgatc
    12061 aaatggttag tgcggttcgc agccgaacac taaaattaat caacgacggt aagcttgttg
    12121 caaccattgg gggtgagcat tctgttagca ttggtgctgt tcaggctcat gcccgcacct
    12181 atgaccacct caccgttctt caaattgatg cacatgccga tatgcgcagc agctatgagg
    12241 gtagccctta taaccatgca tgtgtaatgg ccagggttaa ggagctgtgt cctgtggttc
    12301 aggttggcat tcgtagcatg gatagctccg aaaaaaccaa catagatcct catcgtgttt
    12361 tctgggctca tcagcttgtc gccaacaata gctggatgga tgaagccatc gatcttataa
    12421 ccccaaatgt ttacattaca tttgatttgg atggatttga tccttccatt tttcctagca
    12481 ccggaacacc agaaccggga ggtttgctct ggtaccccac tttgacattt ttaagaaaag
    12541 tgattgaatc aagaaacctt gtaggattcg atattgttga gctttgtcca aacccccttg
    12601 ataaatcttc agactttctt gcagccaagt tgctatataa aatactgtcc tacaagtact
    12661 tgaatacagt cgtctgaatt ttagaaagta taaccaacct gaattagata tgaataagtg
    12721 caagtgtgtt aaagttccac ctcctgaggt cgggggcaag gaattcaagc tgaacgctta
    12781 ctatgaattt gactacatcc ccccggttaa ggataatcca tcgttttata aagtttttag
    12841 ccttgaggat aacgttagtg gcagttttag cattaaggca tttaacgagc actttaaaaa
    12901 gtactgaaag agccagtaaa tccccaaagc aatgcttggg gtaggggttt ggtgctagtg
    12961 ctaaaactag ccagagtcgg cctttagtta ttcaggtagt ctatttcgtt gcagaatact
    13021 agacatctaa atggcttgtc catttttaac agggctggcc gggtgtaaat agttgaattt
    13081 caagtcttcc aaaagttagc tggcaatttc tatcagcttg ttcctatccc taataaccac
    13141 ctcgcgtcgt tttatgctta taataccttc gacctccaaa tcggagaaaa cacgggcaag
    13201 ggctggtcgg gttaccccaa aaaattctgc cagctcctgt tgtgatttgg ggagtatcag
    13261 ggtggcgtta gccccaccgt aggaaagatt tagcaagtaa tgtgcaattt tttctttaat
    13321 ggttttgaag gacaggaacc aaagcctatt ggagagaaat tgagttctgt ttgatatgga
    13381 attcaagtag tttataagga ctgtggcgtt acgctgcatg agcgctagga aatcggcttt
    13441 aggaatgtag agtatgcggc aatcgtcgag cgcaaccaca tcaacaggat actggttatg
    13501 ttcgccaaaa ataaaggcgt gtgcaatggg ctttggtgcc tgcatctcct caattttcag
    13561 tattttaccc gaaaaatcgg ccatttcacc cttgacactt ccctcaacca atatggtaag
    13621 gcttttcacc tcatcgttcc gctgggctac cagctgccct ttactaaaat ttttcaccac
    13681 atgtggggtt tggcttatca tgttttcaat ctctacgggg ctaaccccca tgaaaagtgg
    13741 tgattttgac aaaatcgggt acatatacga gtaggtttag taaatttttg catgaatata
    13801 gtggtaatta cgtaaattaa cttcaaaaaa acattcctcg tagttggggc gaaattcgtc
    13861 gctcgggtta aatggtctat agaagatctt atgtttgtta tatttttgaa ggaatgccct
    13921 ataattgtga taattgctat gccatgaacg accaccaaac tggcaagcta gacatttcgt
    13981 ttaataccga cctgccggaa ccattagttt tcagcacatg gaagcaccac ttgaactata
    14041 tccacgatgc cattttaagc attgaggtag gcagcgaaac aacttctctc atcaacgaag
    14101 ttgcagcagg cgttggaggt acactggttg atttatacat cggagagtta accccaatgg
    14161 ttattgccga agaggttatc cagcagcttg taagctttga cgcattgggc tatgatagct
    14221 tttcccgatg gcttggtgtt tccaagagcg ggtaccagaa catgtacctg agcgacgggt
    14281 cagcatggat tttaagattg ggtatggtta accggcgtta cgttcatatt catcctgcca
    14341 gatactcgtt gaataccgtg cggtgccggt cggccaacct gaaatttgct atcgcatacc
    14401 ggttgttata cggtatggac gattcaaatt tttcaatcga caaggtaaat aggactagag
    14461 aaaccttggg attaccacct gcagctgaaa aaattgccct ctcgggggca ttaagaatcc
    14521 ttaagcagct aagtgttaaa attgattaaa attaaaccag ctcaataata taaccttcta
    14581 tatgtcagat aaatcaacca ttccaaaaca aatgttttat atcatatttt tggtctgaaa
    14641 tttgtgaaac atcattaaca attttacgtt attagtattg gttttaggta caagttttag
    14701 ggttaatagt tggttaaggt ttaggttagt tttaggttta ggaacggagc tgcttttaag
    14761 ccgctccgtt cttattttaa tagcttaata tctagtgcgg cagctctcat aatatctttg
    14821 gtatttaaac gggtaaaggt aacattgtcc tcctaaattt acgattctgc gggcacaagc
    14881 ttactttgtt gcatggatta agcgtaaaat ttccttattc aacatttaaa agtttccggg
    14941 ttgatttctc ttcgacttcc tgtttgtagg gtttctaaaa attatgtagg tttgaatttg
    15001 ctaaacatac ctgttgatga ggcgcaactc cttcctatac gcgttaactt tttctgttat
    15061 ttgctatgcg tgcggtagca aggaacgtaa cgtcacctcc gagcaacagg tttttaggta
    15121 taacgagagt aaagggatat ccactctcga tcctgcattt gccagaaatc ttgcgctaat
    15181 ttggccggta acccagctgt tcaacgggct tgtgcagctt tctgactcgc tagctgttga
    15241 accctgcgtt gcaaagcaat ggcatgtttc agatgatggg ttgcagtaca cctttttact
    15301 cagggacgat gtttactttc atgattctga ggtttttcca aatggtttgg gaaggagggt
    15361 aaccgcttct gactttgttt actcctttag tcggttactt aaccctaaac ttgcttctcc
    15421 cggatcgtgg gtgctatcga tgctcgaccg aaactttgcg ggatttacca atgggtgtaa
    15481 ggctttaaat gataccatac tgataatcag gctttccaaa ccttttcctg catttctggg
    15541 cttggtgagc atgccctact gctttgttgt tcctcatgag gccattgaac attatggtgg
    15601 tgaatttggc cgaaggcctg ttggaacagg ccctttctac atgaagtact ggcgcgatgg
    15661 tgaaaaattg gtgcttcgtc gtaatgccca ctactttgag cttgacaatg aagggaaacg
    15721 actaccttac ctggaatcgg tagtggtaac ttttgtggtg gataaacagt cagaatttat
    15781 agagtttatt agcggaaaca tcgatatgct ttccggcgtt catgcctcct caaaggacga
    15841 gctgctaacc cgaaccggca accttaatcc gaaatatagg gaaaaggtac taatgctcac
    15901 cggcccctat ctgaatacag agtatctggg agtgctcctc gactccattg ggctggagag
    15961 cctagaaaat agtgcgctgc acgatttacg ggttagaaaa gctttaggtt atggctttga
    16021 caggaatgcc atgatgcttt acctccgggg aaatttagga tttccggcaa ccagggggat
    16081 tgtacctcct gctatgcccg gttttgaaac ttcaacagag ggtttttact acaaccccga
    16141 taaatcaagg cagctactag ccgaagctgg ctatcccaac ggaaatgggc tcccaccaat
    16201 taccatctca acaacagacg attaccttga cgtgtgtgag tttatccagc accagcttgg
    16261 cgaggttggc tttgatatta ggattgatgt gctcccgggg gctgcctacc gtgacatgat
    16321 ggctaattcc aaactatccc tgtttagagc ctcttgggtt gccgattatg ccgaccccga
    16381 aaactacctg gccctttttg tgagtagcaa tttttcaccc cttggaccta attacaccca
    16441 tttttcttca tcggaatacg atcaacttta tcgtaaagca ttagctgaac ccttgtatga
    16501 taatcgcttg ccatactacc gggcaatgga ttccttagta attgccaatg ctgctgttat
    16561 acccctgtac tacgataagg tggtacgatt tatttctaag gatgttgagg gtatcacctt
    16621 aaatcctatg aatttgcttg agctaaaacg ggtcaggaag tcatactcgg gtcttgcccc
    16681 tagctgattt tttatggtat taattctaat tcacagataa aatgattgtt gttgccgata
    16741 gcggggccac aaaaaccgac tggcgaatta ttatggaaga tttttcttca gcatcgtttg
    16801 aaaccgttgg ccttagtccc tactttaaca ccgagctgga ttttaacgac gccttgacgc
    16861 atggatttcc taaagaattg aatcctaatg aggtttctaa agtttttttc tacggtgccg
    16921 ggtgcggaac ccaggagcgc ggcagcagag ttgagagcta ccttcactcc ttttttcctg
    16981 cagcagaaat atttgcttgg tccgatgcgc tgggtgccgc ccatgcgcta cttcacaacg
    17041 atcagggtat catagtaata ctggggactg ggtcaaacat ctcgtactac gacggagcta
    17101 agctggaggc taaaacgcca tcgcttggct tcatcctggg tgatgagggt agcggagcac
    17161 acatgggaag gctgctgctc agggattttt tttatggccg catggacagt gttttgggaa
    17221 aaaggttgtc ttctatttat gacatggatt tagcacatgt tttaaatatg atttacgata
    17281 gccctaagcc ttcggcctat ttagcttcgt ttgttccttt tctgctcaat aatgtagaaa
    17341 acccatacat atcggattta ataacccgat cgtttgagga gatgtaccag ctgcacctga
    17401 gctgctatcc caatatacga agcattaaaa ttggtgtaac cggatcagta gggtgtttgt
    17461 ttagcagcat tctcgatagg gtggctagtc agcatgggtt taaggtttac aaatacgttc
    17521 gctacccaat cagctctctt gttgattacc acgtcaacct ataccattcg gggaggtaaa
    17581 ccctgagcat ttccagagtg tttcagcgta ttccctgcat aacatcgcct gtttttcctc
    17641 tctcatttcc gaaagcactc gggctaagcc attcatcagc ttcatttcaa caagtttgta
    17701 gcctgcaatc ccgctcagct caatggcatt cctgatgcac tgatgggcca tagtaaggtt
    17761 ttgggtatga aagtagctgg cagccctacc aacttcgcct gcaattactt gcgggaagct
    17821 tcgaataggg ttgagctgtt ggtatatttg ctgaatttgg cagtcttctt cggctgagaa
    17881 gtggttttta ccgctgagta tttcaaagag ttttctgttg aagtagttaa caacctggta
    17941 atgcgatcct gcaatggagc tgatgtcttc aagatagttc tcaactgccg ggtgttgccc
    18001 taggagaagc agggttggaa caataagttc cgaaaattct gcccgctcgg cataagaggt
    18061 aaattttctg tagtatgaag tggcgcgttc aactatcggg gtggtattat tgccattgcc
    18121 catgattatt tcggcaaaga gctggtttga aaaccacgct cctgccacta gcggtgaaac
    18181 atctgcgggt ggcttaacat tacgtgttgc ctcgtggtta ggttgtgcta gcattccgtt
    18241 tagggttatg aacccattga atgcaagaag agtttgggca aaaaagagtt cggaaatgtc
    18301 ggtagtgttg gacaaatagt gcttaagcac agtggtaaaa taaccgttaa ggtggtttag
    18361 gtcaatgttt tgctccacca ggagctgtcg ccctaatcgt gttttggtat attcgggcat
    18421 gaggacgttc aataggtagc tgctattcct gagcgtagcg cagatctccc ttaatagctg
    18481 gccatgggtg taggcgtttg gacaaagttc aaaaaatttt acgagaactt tttcgtttcg
    18541 gtctctgctg ccggtgtagt agagagttcc ctgcacgaga gggtagaact cggtacctgc
    18601 aaatttttct tctacctgcc gaaaggtttc ttcagtgatg cctccggttg agttaacaat
    18661 atcctggaca ataagcagcc ctgttagttg agggcttgtg atgcgaacgt acttaaaata
    18721 ccggccggtt gattcaacaa cctgttcttc ggtgataatg ccgtaggaca ggagttcatg
    18781 gtatgctgta aagtagttac ccgaaagttt aagatgtata gggtagcttc gcttaatgtc
    18841 ttccttcaaa acacgatggc cattgcccag cctgattatt tccgttagga tatcaacttt
    18901 ttcgtcgggc agtgtagaaa agtaaatttt actcttaaga aactctaaga gtatttcgtg
    18961 ctggttattg agcaaattct gcttctcagg ggtgtaaacc tgaataaaca gctgaagaaa
    19021 gtaaggatag ctaagcgttt gccttaaatc gggatgaagt tcaaaaataa gcaaacgctg
    19081 gttgaatctt aggtttatgg tttggtcaag gatttgctga acttcgtcgg gcgaaagcgg
    19141 aggaatattg gctccttcgt gggttagcag ctcagaatcg gagaaatacc aaagcagtgg
    19201 gtgcgtaatg tatggcttta gctgagtcca agttgacagt cgggtcgaaa taaccagctt
    19261 aaacctgttt gtttgggcga aagcctcagc caggtcggca agagcttcga gtatcttgtt
    19321 gaacttggct cctaccgaca ctacctcgtc cagagcatca aagatgaaga ctattctacc
    19381 aggaagatct tttacaagta ggctttctaa cgatttttgc ccaagaagct gtgccagcca
    19441 atggccgata aaagtgttgg agcttgcgta atggtcgagc atggtcgccg gtgccagtag
    19501 gaatacgtcg ttcatgttag cctttaaatc tctgtgtatc tggtaccaat gggcgagcag
    19561 gattgattta ccgtagcctc cgggagctat aatagcagta gcagggttgt tgctgttcaa
    19621 aaattggtca aggcgctctt ccacctgccg tcggtggact gtctcttcaa acctaatgcc
    19681 tgataaatgc atgatatggt tgagtgtttt actgctgacc gctttggcat gttcagcggc
    19741 ttgctcccaa tcttcatcag ctgaccgaat gattccttca ggaattttgc tctttttgtg
    19801 actgacaaaa tcatcccaac ccgtatatcc tgcatatcgc gagagaatat caagggttac
    19861 atgcgatgga ttagaattcg tagctaaaag accgaacagc cttcgtaggg ttgccgggct
    19921 cagatactcc ctacatgtat ccattatact gatagagagg gagatacagt ctttctggca
    19981 gcttattttc ctgccaaacc tactttcaac ctcctgagtc agcagctcaa ccatggtatg
    20041 gtactatgtt tattaagcta actgaaatgt ataaataccg ttaactatgc aaagatatgt
    20101 gattgtttag atagcaaatc tggaaatttc taaaacttga cagctttgac acttttcctg
    20161 gtaatctgaa atggggtaaa tttgaactgg caaacatttc atatgtaata agtgggggta
    20221 agcaaaccaa tcaaaatgaa atgtacttgc ggggagtaaa ccagggttag tgtttaagca
    20281 aagataagtt atgaagaagg aaaattccat aagggagaaa ataacgctga agtttaagga
    20341 tgatgccggg gtgtttacca tctttttttt ggtaaccctg ctttttgtat atgcgcttat
    20401 taaggcgcta acactttgat gttaacagac taaaaaaact tacggttatg ctatcacaag
    20461 tttgggaaca atgccacgca tcagtttgta gccttcactt cattaatgaa aggggaatgg
    20521 ttattgattc tctttccgga ttcaaggtta acagttcgtt gataaccagc gagtacgcat
    20581 tttacataaa caatgcccaa aaggtggaga tttcctttgt tgaaggcgat gccaatactc
    20641 caacggcaac catgaggatt ggctacaacg aatttattaa cgatttacga attggctaca
    20701 ccaataacca gtccgattac gcagttttca acataaattt ttctgaattt gaaaaaatac
    20761 ccggtttaac cctgtgcgag tgtagaaatt ttcaaattgg aaaacaagtg gctacattgg
    20821 gtttcaactt tggcgaaaac aacctgtcga taaaatccgc tgttatttct tcggcatttt
    20881 caagtaaaaa tgggattaga tgtcttgaac tggatgggct tacttgcttt ggaaatagcg
    20941 gttcgccggt tattgacccc gatagcatgc aggttatagc aataatctca cgaagaaaca
    21001 ctcccgcatt taagtcgtac atggagctaa acaggtgcat tgtttcaaac attgaggagt
    21061 taagaaagct ggaggggaag ctgaaaattg atacgattga cccggttcag gtgctaattg
    21121 ccaaccaaaa ccaaatacgg ctgttggcat ctaatcttta caagtacacg gctacaggct
    21181 catcaaaggc cattatgctc gaccggatac tttcctattt caacgagcag gccatcacag
    21241 agcaggcaca aatctcttct ttagaacaaa aggttggtta tggtcttatc gattaatggt
    21301 tagttggttg gtgagtgctt gctaaaggcg tcccgtaagg gatgcctttt ttcctcctat
    21361 aaggtagaaa aatatccgtt tctcattaag tacaacgggt attaatggct tttgcagtat
    21421 aattcagctg aacgaatttc tgcttttcct gttttattag ctaacattta gtaaattgca
    21481 ctaattcgtg gtaaaacacc tttgtaaatt ttaaattata gcacaatgaa taccaaccag
    21541 atgatagaat ttttaaaaaa gattgagctt ttccggggcc ttaacgaaaa ggaacttgaa
    21601 agcttagtta acatgatgga ggtgattgag tttaagcctg gcgaattgct gtttagggag
    21661 aatagccagc gggtggggat catggtaatt tatgagggcg aagtggagct ttttaggagc
    21721 aacccctttg gggtggagct taaacttacc tactttaccc ccggcgattt tcttggcgag
    21781 ggttcttggg caagcaattc gcctcactcc acctcagcca gggccctaaa accaacaaaa
    21841 gtattcgtgc tgaaggataa tgtgcttagc cagcatgcgg aaatcgggtt gaaaattctc
    21901 atcaacataa cgcgtgtaat acaacgtcgt atgcacaatg ccaacaaccg gatggtagat
    21961 gcagcttcgc agtatgaatc gggcagcacc agggttgagc atgacctgct aggcgaacgg
    22021 gatgtccctg cggagtttta ctatggagtg caaacccttc gtgccctcga aaactttaac
    22081 atttcggggg taaccctgaa cttttaccct gatctgattg attctctggc caatgttaaa
    22141 caggctgctg ccgaggccaa ctttgagctg ggacttattg acgaaaaggt tagggatgcc
    22201 attgttgctg cctgtaagga aattactaac ggtaagctgc acaatcattt tgtggttgat
    22261 atgatacagg gaggggcggg cacatcaacc aatatgaatg ctaacgaggt gattgccaac
    22321 agggcactcg aattaatggg ctactccaaa ggtgagtacc agcattgcca cccgttgaat
    22381 catgttaacc tgtcgcagtc aaccaacgat gcctacccca cggcggttaa gctggctata
    22441 gtgcgctcca acgccaagct gattaaagtg cttgaatccc tcatcgagtc gttccgtaag
    22501 aagggacaag agtttgccca cgtcatcaaa atggggcgaa cccagcttca ggatgctgtc
    22561 cccatgactt tagggcagga attcgaagcc tatgccgtaa ccctggggga ggaaattgaa
    22621 cggctaacac agaacgcaaa gctatttctt gaaataaaca tgggcgcaac tgctattggc
    22681 accggcatta actccgatcc ggagtacccg caaaaagtgc tgaagcacct taggagcatt
    22741 acggggctgg agctggtttt ggccgcaaac ctggttgagg caactcagga tacaggtgca
    22801 tttgtgatgt actcctctgc tgtgaaaagg ctggctgtta agctttcaaa aatttgtaac
    22861 gatcttcggc tgttatcatc gggaccaaga accgggttaa gcgagattaa ccttcccccc
    22921 atgcaacccg gttcgtctat tatgcccgga aaagttaacc ccgtcattcc cgaagtggtt
    22981 aatcagattg cttttagggt gataggcaat gatttaaccg ttaccatggc tgccgagggc
    23041 ggacaactcg agctgaatgt ttttgaaccg gtcatcgtgc acagcatttt tgaatcggtc
    23101 gaaatgctga aaaatggtat gaatacgcta aaatatcgct gtatagatgg gattactgcc
    23161 aacgaggaac ggtgcaggag catggttcac aacagtattg ggttggttac tgctcttaac
    23221 ccctatattg gctatgaggc ctctacccag cttgcaaaag aagcgctttc atctaaccga
    23281 agtgtttacc agctggtgct ggaaaaaggg ttgcttacac gggagcagct cgatcggatt
    23341 ttagcacctg agaatatgat aaaaccgggc aaaatgaaac tctaaattcc tggagctgat
    23401 gggggatttt gatacttgac tgcttaatgc taaggcgatt gtgtacaatt ttctccaggc
    23461 atgtttagtt ttccttttaa agtaaatagt caatagtttt ttattctaca tttgtggggt
    23521 aaatgtaggt tttctaataa tttgcattcc atgagcgaaa gtaatagctt tcaatttcgg
    23581 tataaccggt tgaggggcaa agaagagtta gctcccaacg aaagagaact ggttgacagg
    23641 gctgttgctg ccctgtcgtc gagctactca ccctactcac actttcgggt aggggcagcc
    23701 gtgctacttg agaatgggga gattatcacg ggcagcaacc aggagaatgg tgcttaccct
    23761 tcggggctat gtgcagagag ggttgcgttg ttctatgccg gtgctcgata tccgggaatt
    23821 cctgtagttt caatggctgt tgtggcaagt tcgggagatg gacttacgga gaatccagta
    23881 accccatgcg gagcatgccg gcaggttatg agcgagtaca gggcagtttc agggaaaccg
    23941 tttgccattc taatggttgg gagtaacgaa attgtcaggg ttgaggatgt agagcagctg
    24001 cttcccttta gtttttcact ctccaatggg atgaagcgga agtaaggcct ttgctgaagg
    24061 ttagaaggag gccactattt tcctgaagga ggagttgggt attagcccaa gttcatcctc
    24121 ggagtactcg ggttttatgc gctccacaaa gtacatttca atttcaccca ggttttttgc
    24181 gggaaccttt ccccgcgaag tgcaaacgaa gtaatcctta atgtaggaat atgtggtttg
    24241 cgaaatatta acttttccgg gttcgctggc agtctccatt cggctggcta tgttcaccgt
    24301 gtcgccccaa atatcatagg cgaatttgcg ttttccgatt actcctgcaa ttacacttcc
    24361 ggtgtgaatg ccaatacgga ggttccatac cggtttacct tccattgcag cctgtacagc
    24421 cttatcatga ataaatcgct gaatctcaat ggcagcaagt acagtgtcga atgggtggct
    24481 acggtttact ctggggagtc ctcctacaca catatagcta tcgccaattg ttttaatttt
    24541 ctcgatgaaa cgctgggagg ttatctcgtc gaatacctca aagaaaccac ccaaaacact
    24601 gaggaactcg tctatatcac catagttgct gctgattttt gagaacccaa caaaatcggt
    24661 gaacattacc gaaacctcct tgaacttctt aggtttgtat gatccctttt tctgaagggc
    24721 atatgcaacc tcccatggaa ggatgttttt cagtaaattt tcagattttt gtttctcctc
    24781 ctcgagctgt agggcttgtt tctgaattct ttcctgatgc tctgccagca cggcgttaac
    24841 gctctccagg ttttcggtga tggtaagcag gtgttcatgc ttttcctcca ggtctttttc
    24901 aatggctttc aggtcggaga tgtcggtttc gatggcgatt atttggctta agttctggag
    24961 gctatcaaaa atcggcgtaa gcgatgtttg aatgaatttc tccttgccct gaggggtaac
    25021 ccatttgctc tcataaacaa catactcgcc atttttccgg catttgtcaa gtgcctctcg
    25081 tgtaaccggg tttgttgagg aatcaaatag gttgtcaccg aaaatggata caaagtagtc
    25141 gagcgggcat tcgtatagcc gtttaaaccc ttcgttaacc caggtaattt tacccaggtg
    25201 gttgataaca atcatggagt taccagcctt gctaatggca tcagtaatgc tgttgaactg
    25261 ccgggattgc tcttttagtt ttttcagcat tcttccccgt tgaatgccta tcatggtccg
    25321 ttgaataagc tcagcagcag caagaggctg gctaataaag tcgtctgcac cggcaatgaa
    25381 aagggcttgg attctctcag gttgtaagaa atcggctatg gccagtatgg gtaccgattc
    25441 ggtttttata tcgtttttaa taatggtgag cagatctggc gctgcatact ccgaatagga
    25501 aacatcaaaa agaacaatat cggggttgag ctgcgttagg ttggtgatga tattcgtgtc
    25561 gtcggtaacg aagtgcaaat caaaatcaaa ttcggcaatt cccacgatgc tcttaatttc
    25621 acgggagagc ctttcagacc cacaaattgc aactatgctg taagccataa tgctctattc
    25681 aacctatatg caaataaagt taaatttttg aaatattctt cagggttttg cctggaagtt
    25741 tataggagta gtttaggaca gagagaaggg taaaaatatc attaacaatt ttttgataga
    25801 actatttgta gatgttgatg aaggggtaca cattgtccaa cagtcataaa gggcgaatga
    25861 ggcttgggca gctcttttag ctatcctctt caaaatcgct tatgattttt tgtatttcct
    25921 cctcggtagc ccggagcttg gttttgcaga atttgagcag ctcagaggcc cttcttatct
    25981 tttcggtcag gtcgtccacg tccagctcgt tgttttcaat ttctgtgatg atttgctcaa
    26041 tttcgttaat ggcatcaata tacctttgtg gctttttggt catagttcta attatttgag
    26101 ggtaatgaaa tggtgaatgt tgttccaagt cccggtgtgc tgcttacctc aatagttcca
    26161 tggttcaact caacgaattc cttacagagt attagcccca atcctgttcc gttttcctga
    26221 ttagtaccct tggtggtgaa cccgtcaacc ctgaaaagtt tggcctgatt ctcaggatca
    26281 atgccaacgc ctgtatcggt aatagcgatt tcaaccatgc tgttacttga attttttgct
    26341 gatattccga ttgatccacc ctgcggggtg aatttaatgg cgttgctcac caaattcctg
    26401 ataacggttt ggagggtatc gaaatcggca aaaaccgtta ggttggcggg aatgctttcc
    26461 cgaagtttga ttttcttttc ctgtgcagga atcgcagcta ccgaaaaaac ctggttgata
    26521 aggtccttga gattaattgg ttttggcgag agctcaattt ttccggtttg gcttcttgcc
    26581 cagcttagaa ggttctcagt caggttgagc acctcggttg ccgagcggtg gatgtggtgg
    26641 ctgaactcac gaatctcatc gggcgaaagg ctctccaggt ttgttgccat tacctccgat
    26701 agtccaacta aggcggtaaa tgggctccgc aagtcatggg caatgattga aaatagctta
    26761 tccttagttt taatggtttg acgcagctcg ctctcagact ggttgagcct ttcgtttacc
    26821 agcctgattt cctcaatttt ctcctcaagt agcttggcat aacgccgttt ctgaataagg
    26881 ctgtagaaag ctatgctggc cgcagttacg acaagtagga ttatggcaat tagcgagttg
    26941 cggaataatc tttgcttctc gattatggct ttatccagag cagtcttctg tctgagcgct
    27001 tccacctctg cttccataac cttaatctcg gtttttgttt ggtcgagctc caggtctatc
    27061 tgagcctcta ggttcgcctc gtttctttta acgctctcta ggctgtcgcg aatgcctata
    27121 tgctttatgt agctttccag ggctttatcg aaaaggcctt gtgcgcggta tagctcactt
    27181 tgagctagat agccgtatga tagaaggtat ttgttgtctg attgtttccc aatttcaatt
    27241 gagctgttga tgttttttct tgcctcatcc agctgttttt gcccaacctg aatttctccg
    27301 atttttctta ggcaaagggc aactccctcg ttgttattaa gagctttata gttttcaatg
    27361 gctgtgtggt aatgcttgat tgccatgcca ggatttttag cctgtcgttc aacattaccc
    27421 agctcgttgt atagatctgc aatatcgctg gtgctatgtg ttcgggttac caacttgagg
    27481 gcttgtgaaa ttgatgtacg agcttggtcg ggttggctgc ttttaagctg ggcctgggca
    27541 atgttcttta aaattttagc agcgcctatg ctatcgccgc ttttttcttt aagagtcagg
    27601 gcttccttat atgtggcaat tgccttgtcg ggggagttga ggtcaaggta gatatgcccc
    27661 aggttttgta ggatgtatgc agcatcattc atgctaccct tcatgtcctg aattatttcc
    27721 cttgcctgtt caaggtattt aagggcgctt ttatacattc ccaaccttcg gtaggtaagg
    27781 ccaatgctgg tgagcgattt ggcaatatcc tcatcctgcc cgtaggtttg ccgaagtttt
    27841 aacgccatta ggtagtgacg cagcgcttcg gctatattgc cctgctgcag gttaaggtta
    27901 cctatttctg ttagggttga tgcctctgcc agcgggtcaa caacttgttc ctgaagggcc
    27961 aaagcttcat tgaggtgttc gatggcctgt ttgtacattg atttttgcaa gtaggcacgg
    28021 gccatattgc tatgcgttga aatggccgac cgaagtaatc ccagtttttt ccttatttgc
    28081 aggctttgtt cgtattgctt tagtgcgtcg tcaacctggt tgaatttaaa atacagactt
    28141 ccaataatgt tgagtatggc tgcctcttcc gtatagttgt tactactatc cgacagtttt
    28201 aaggctctgt tgaggtaggt aagcgatttc tcaaactggc caagatcgcg gtaggatatg
    28261 cctacaccct tcagggcggc tagcatcccg atagtatcgt tgatatcctc gaagagaatc
    28321 agcgatttaa agtagctctc gagtgcttcg ttgaagcgtc cttgttgcca gtatgagctt
    28381 gcgatgtttt gctggcattc agcctgacac tttttgcaac cagctttact agattggtca
    28441 agggcaattt ccctcatcct gtttgcctct gccaggtttc ccttttgctg gtaaagcccg
    28501 gcctggagca ggagaaactt tacacccagg tcaggccttt tacttttgat agcttggctg
    28561 tatgcttttt gtagcatttt gagagcggca tcgctgcccg aaaccttaac ctccaggttg
    28621 cttctaagat ctactaaatc ggcttcgaga ttcaaatcgt tgagtttttg gacaaaaagc
    28681 gaagccgaat caattttctc gctggcgttg ttccaaagcg acatggcaat taaggcctgg
    28741 gcatttgcca tgagcgatag cgctttatca cgactatttc ctgatttaga tgattcctgc
    28801 ctgcacaggt tggcaagcct gctggcttcc tgtggatttt cgtcggctat tgccagcact
    28861 ctttggtata ggctatccac cttactgctg cttggtgaac ctgcctgagc agctataagg
    28921 ctgtaagcgg taagcagtag ggctacaccc catttttgaa taaccagctt catgttttgt
    28981 gttcagaaaa atcggtaaaa aatacaaaat tatactggac tagaaatggg ttggctgatt
    29041 aaaaaattat gataaaatta ggaggggtgt gcgtgagctt attttgctgg aagggtgaag
    29101 aaaaaggtgc tgccctttcc cttttcactt tccacccaga ttttaccgtt gtttttttca
    29161 acaaactcct tacaaagcag tagtcccaaa ccggtacccg attcgttgga tgttcctttt
    29221 gttgtgaatg agccatcaag catgaaaatc ctgtcaaggt cattatcgtt tattccaatt
    29281 cccgaatcgc ttacagatgt ttcaaccatt tgatccttat agcgtgcggc tatctctatg
    29341 tggccaccca aatcggtgaa cttaatggcg ttgctaagca ggttgcggat tatagtagcg
    29401 gttgtgttct tatcggcaaa cacgctaatg ttctcgtcaa tgttaacggt aatgctgatt
    29461 tttttcttct cggagttcag gttgacgttg gttatctcct gcttaacgag aggaagaagc
    29521 ttgaagtttt cgggtatgta ggttattgtt ccggtctgag ttctggtcca ttgcagcagg
    29581 ttatcaagca gcttataaag cgattgggtg gaatcattta taactccaat gtactcaata
    29641 cgctcctcat cggagaggaa gttgtagttg cgctcaagga tatcgctaaa tcccagcagc
    29701 gcattgaagg gatttttcag gtcatgagcg attatagaga atagcttgtc cttggtggca
    29761 tttagctccc ttaagttaac ttctgtttcg gccatttttt tattcgcttc ttccagcgcc
    29821 ctgttctttt cggcaagcag caagttcttt tttctgatga tgcgtgaaat gtatatggta
    29881 accgcaattg ctatggccag taggatggtg atgaacgtta gaatgttttg aaaatttcgc
    29941 tgcttctcaa actcaagctg ctttatttcg ttgtcctttt ttaagagttg tatctcacgt
    30001 tcctttgctt cagtttcgaa cctggtttga gtttcgagta tgtttcggac ctgttgctgg
    30061 ttgtatatgc tgtcgttgta tgccagctgc agcgacttaa actcgaatgc ctttttgtag
    30121 tttccacgct tggcatacag ctcactgagc agctggtagg tttcctgaat gtaggccagc
    30181 tcggttatac ttttaagatt gccaagaact atattcaggt actcctcaca cttgtctagc
    30241 attcctgctt taaggtaaac cgaggcaaca ttattgcaag tattaaggta cgactcaatt
    30301 tcgcctattt gccttgatat agccatagat ttcaggtagg cgttgatgcc tcgctccagc
    30361 tggttgagct tcacgtatat aaacccaata ttgttgtagg ctgtagaaat acctatagaa
    30421 tcgttaagtt gtatggcaat atccagcgag cggttgtagt tttcaagggc cttttcgtag
    30481 ttccccttct cgtcgtaaat tattcccagg ttgtttaaag cagatgaaat accggatagg
    30541 ttattcgctt ttgaccaaat ttcataagct ttaagatagt aatcctctgc caaatcgaaa
    30601 ttcttaaggt ctttatgcag gtttccaata aagttgtagc atcgggccag gggttgagtg
    30661 atgtcatgct ccagaaaaat atttattgcc aaaatggcgt tttcaagagc cctgttaagg
    30721 tcgttgagct ggcggtaaac ctgggcaatg tcaaggtatg aaattccctc aaagaggtaa
    30781 ttcttagcat cactgtaata ttttgctgcc tgcctgtagc tgctgatgga ctcgctgtat
    30841 cgcttgagat ctcgtagcgt caatcctaga ttgcttaatg agtggcctac ctttgtccag
    30901 tcaccgctct tctcacggta gtctttcgat ttaaggtagt aggtaatggc cgtttcgttc
    30961 tcatgctttg atgagtaagc attacccata ctgttgtatg catcgcccag cgcttcgttg
    31021 cttttgtctc gttgggcttc aaccaaggcc aggttggagt agtatagcga tgagtcagga
    31081 tttttctccc agtagtacct tgaaatttta tcaagcgcgg ttatcttcct aatgccggtt
    31141 gtttgtttgg cctcggtaag taggctgtca agcagatgca cagaagagct gttttgtgca
    31201 aggaggatgt tgccggtatg aaaagaaaaa accgaagcaa gtaatagtat atgtcgaaat
    31261 tttttcaagt gccagagctt tgttggatgg ttgtctgctt taaacatcaa attctaaatt
    31321 tactattttt tattgttagc aagcaacttt ttgcttgctt ttatttttgt aatgttactt
    31381 tctatgctcc cattccaaaa ttgagtggta atcacatcgt tgacgtttaa attgttcaga
    31441 ttttttacaa ttttcccgtt ggaataggtt atcgtaaagc cgcgatgaag gatgctgtcg
    31501 gggtgcaggt tcttcagtgt gctaaagtgg tgctcaattt ttattttttc gttttgcagt
    31561 agcttatgcg gcatgctttt gagcaaacca gcataagcgt ttagcttaac atagttggcc
    31621 tttattctgg aaatgggaat cgaaagcctt atattcaaac ttttaagctc atcagcatgc
    31681 agcacctgaa ctagttttgc catatgaggt agtgagctgg ttagctgttg aaggtatagc
    31741 ttgttttggg cgactttttg gtttagaata gcttgtagct ctacttgatt gctggttagg
    31801 ctttccttca tgtcggcaag taattttcct gcgagttggg aaacactccc ggcaaggttg
    31861 tttgcaacag cttcagcatc acggaattta tcaataagga attctgccac agcggtaggt
    31921 gttttaagtg atgtgtggga aaccatgtcg gcaaccgtaa cgtccctatc gtgcccaatt
    31981 cctgtgataa ccggaatggg aaactgggca atgtttgagg caagagcata cgaatcgaag
    32041 catgagaggt cggtttgagc accaccaccg cgaacaatgg ttacgaggtc gaagcaggcc
    32101 agcttgtcgt aaattttgcc cagggcgttt atgatagaca gctctgcttc atcgccttgc
    32161 attagtgctg gaaataactc gtaggtaaag gcgtagccat gggggttatg ggtaagttgt
    32221 tttacaaaat cctgaaaccc cgcagcattt gccgatgaga tcaccgcaac ccgttgggga
    32281 agcatgtcca gctccagctc cttgttcatg ttcatcacgc catcggatat caacctggca
    32341 ataacctcac gacgtttcag ctcgatatcg cccaacgtgt attcaggttc aatgtcgagt
    32401 acggttaggc tgtagccgta gtactcatgg aatacaacct gtgcgttgac cagaattttc
    32461 attcccctgg aaagtggccg attggtggtt gactcaaagt agggtttcag cattcggtag
    32521 gtgtagcccc aaatgtttgc ccttgctttt gctcttatgg tatccgtgtc atctcccttt
    32581 tgcacaagtt ccatgtagca gtggccggat ggatgttcgg taatctcgct aatttcgcca
    32641 accacccagt agctgccggc aagcgacttg gccaaagtct cttttaccag caggtttagc
    32701 tccagaaggg ttaagcgggt actgccgttg ctcatcgggg ttagtcttta atagctttca
    32761 aattgaacgc ttggttaccg gcagcgatat ggaaaatata gaggcctttt ggtaggctct
    32821 ccggaatgtt aatacgtaaa accccatttt cggtaatctg cctacacata tagagcgtta
    32881 ggccgctcgg ggttagcagc tggcagctta cagcttcgtc aatctccagc ggaagatata
    32941 tttgcacttg gctgctaaac ggatttggaa tgattctgat gtttggtttg tagctgtagg
    33001 ttccaaccgg catggccagg gcaaagtcag gaatgccgta cccgatttca ttattagggg
    33061 aattgaatga acttgaggct gaaactaccg attccattag ctcgttgact gttaggttag
    33121 gttttgcctg ccataggcaa gccatcatgc ccgagattag tggagcggaa aaggacgtgc
    33181 cgtttccgct gcccaccagc ccatcggtag tagcaataat cgttccaaca cccattgcgc
    33241 ataagtctgg ctttacacgt tcatcggctg ttgggccaaa ggagctgaaa gcagccttgt
    33301 tcctattgcc atccactgca ccaatggtta gcaccttagg cgagtcggct ggtgctgaaa
    33361 tgtagtacca tggtgatgta ccatcgttcc cggcactcac caccaccagc ataccctttg
    33421 atgccgccat gttagctgcc cgggtaatca ctgtcgtttg cccgtcaagg ttgctgtagg
    33481 tatggttttg agaaggcaca tcaaaagtgg tgtatcccag ggaggtgtta atgatgtcgg
    33541 cacctgcgct atcggcaaac tcagcgcctg ccgcccagtt gtactcttca accagctgtt
    33601 cggagctggc atcctcagtc ctgatgagcc agtagcgggc gtgaggtgct gtgccaacaa
    33661 gctgtccatt ggcatatccc cccatgatgg acagcaccat cataccatga gcatgttcgt
    33721 tatagatgtt agaggtggga ttaacaaaat cttttgttcc cagtatccgt ttgttcatcc
    33781 aaaggctgtc gaaaaccgga tgtgagtcaa cgttgtaaaa cccggcgtca agtacggcaa
    33841 tttcaaaccc atctcccaaa aatccattac tgtgtaaagt ctgtccgttg agcatattta
    33901 cctgtgtggc cgatatcccg tagtttggtg cgtccttgtc aggtttagaa aatggttggg
    33961 attgtggttc ttctattgtg aatttcgagc ttttatgctc cttggttggt gtatatatct
    34021 ctttaattcc ggagacaaag ggaatattct gaatttgcgt cagctggtcg ggagttgaaa
    34081 ttgtagccac aaccccgttc atccaccgaa gcgggaaatg aattgtaata cccatatttg
    34141 caagcgtatc aaggtagctg gggttaacgg gtaaatcgga ttctgttaat gccaaatttt
    34201 gccttgttct acgctcaata gaccttatag ataggaactg ttcggggttc gaaatggtgt
    34261 atggggagtt ggccttatcg gtgaagccaa taaagtactt gctttgaggt tttgctgcgc
    34321 tggataaacc aaccagctgg atgattagta gtattcgaaa gctgtacttc atgggtagtt
    34381 ggattagggg tagttagatc cgtatgacat ttcatcaaaa tcgggttctt ttatcctacc
    34441 cgcattctgc atgagcgagt ccagcaaatg ggtttccctt tcgagaagct cgttgtagtt
    34501 ttcggggaca cccttaacat accttatccg ctgggtgatt ttaccgtttt ctccgtagaa
    34561 tatccattcc ccttctttca gcccattggc atattttccc tccaaacgtt ttctcccgga
    34621 agggtaaaag accttcaggt cgccatcctg aattccattg ttgaagttga ctaccgactt
    34681 atttaagcct tcaggaaaga actggataac agctccatga agcttaccgt tggtgaatgg
    34741 ggcagattca agcagcttac catccggata gtactgcctg caacgtccgt tttttaggcc
    34801 gttttggtag tactcgtcgt agtttatggt gccatccggg ttgtagtagc gccaaaggct
    34861 atccttctgt tgtccaacga atttcccctc ggcgattttg ttccccttag ggctgaaaaa
    34921 cacagcccag gccgtactat cctgtcggta gctgagtatc accttcaaac ttccattttc
    34981 gtggaatcgc ttaaactctc cggtgggctt gttatcctta aaccaaccct cgtatgcttt
    35041 taccccggtg gagtagtatt ttcgccagag gccctgcttc aatccctgct catcggtgcg
    35101 gttgaaaacc gtatcgcttt gtgcttgaat aaaagatggg agcagggcgg ctattaccaa
    35161 aatgtggatt tttttcatca tgctgttcgt ttgctgcaac ctgcacaaat ttagctaaca
    35221 gtttttgatt ggttatacgg taaggtaaaa ttggtttcaa gaaggtggag atttcttgga
    35281 atacctgaac gtgtaggctt tagttatgcc gggctgatgg ataaaaacca gctgttcaat
    35341 tgtttgcccc ctgattgtag ttgatgtgaa cagggatggc cagagtaaag gaagtgaagc
    35401 tcaggctacg cctccgcttc gctttcttcc tttacaaaca aaaaacggga gatgattacc
    35461 ttatgtattg atagaaagat acctttacac actttttggg atgctaccga gttagttttg
    35521 tatttctatt ataatcgact atattcacta acaaatgact actaattgcc caatttgccc
    35581 aactattacc cgatcgttgt tccttttgct ttattcttat aaacgcgatt tctttatcat
    35641 ccggttgttc cggtagttta tctgcaatac cggtagttta tctgcaattg ccggacattt
    35701 attggactca atattaccgg ttttgctggc aatgcgggct ttcagcatta agggattgag
    35761 ccaattatga gttaattgag ccaattcttt ttttgtgtaa ttcataataa ctcccggtac
    35821 ttttcggtta tttagcggtc gtttatcaac cgtttcggtc gtttagcggt cgtttttatt
    35881 tgccaactca taataagtac ctctacctgt ttcaccaatt cttacaaata tctgttgctc
    35941 aactaaatca cgcaattcgt ctattgtaac agatttgccg attccattga tttcctgata
    36001 cactgcgttg gtaatttttc ctttcgtttt tacatattca acggctttca tttgacggtc
    36061 gttcaatccg agtttttcta attgttcttt agaaaaacgg tctttgaaaa gcgttacgat
    36121 aaaaccgccc tctttttctt tcaggtcggg tactggcagt ccatcgattt tgcaggaatt
    36181 tataattttc attatgccac tgccccatga atcaatatat ccgcctttga aacatgcttc
    36241 tgcaagtatc ggattgcgtg ggtgcgacga gtggttttcc ttgagttgcg acaacgtaat
    36301 tgttggtggt aaacctccgt cgttccaaac agacagtttt ttatcataca cacgaatttg
    36361 cgtaggcgca cccatataat ttctgtgaat gagcgcatta agtaacattt cgcgaagtgc
    36421 agttatgggg tattcgagcg tttcgacgcg attcattcct tcaaacgaaa tgttgcgtat
    36481 caggaattta taatcaagtg tgcgtaaaac tttatctagc acttgaataa tattaccttc
    36541 ttctacttct tgaaaacgaa ttgtaaaatc gtcgtcttca aacttgccga tttttacaaa
    36601 ggtgttcgga taaaactttc cggggtcttt gccaaaaagc acgattgcag cgcgtttgag
    36661 ttttccgtct tgtgccaatc gtaatttttc caaaagttcc ggtgtggaaa gtccgtcaat
    36721 atcaggcaaa cgacctactt cttcggcttt tcccaaatat tttttgattg ttggctcgtc
    36781 aatatccgca aaagttgccc gctcgtcaat aacatcatcc caagtataac ctgcccgttt
    36841 gagcaaaaaa tcgttgaggg ctgcgccagt gagttcctgc attacgctac cgctacggta
    36901 ataatacctt cctcgcaggg aaataggcac tgaatagggc tgcacgacaa tttcaatata
    36961 atctttgtca ccctcgtgca aaagattaac ttcggcagta attcccatat tgtttttgat
    37021 tttgttggga atttcttcca tcagcgtttt ttggttttcc acgccgataa cggtagcggc
    37081 atcgttcatg ccaatatata ttttaccacc ttgcgcattg gcaaagccgc atatccattt
    37141 caagtaatca ttgtgccaac tgcttttgta ttctatgttt tgggattc
//
LOCUS       DAOSHM010000023        36805 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_890_length_36805_cov_20.484082, whole genome shotgun sequence.
ACCESSION   DAOSHM010000023 DAOSHM010000000
VERSION     DAOSHM010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 36805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36805
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_890_length_36805_cov_20.484082"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            31..103
                     /locus_tag="PK839_05345"
     tRNA            31..103
                     /locus_tag="PK839_05345"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:64..66,aa:Gly,seq:gcc)
     gene            165..249
                     /locus_tag="PK839_05350"
     tRNA            165..249
                     /locus_tag="PK839_05350"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:199..201,aa:Leu,seq:taa)
     gene            395..1759
                     /locus_tag="PK839_05355"
     CDS             395..1759
                     /locus_tag="PK839_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HOG72198.1"
                     /translation="MRVHFIAIGGSAMHNLAIALKQKGYEVTGSDDEFFEPSLSRLKQ
                     HGLLPSEIGWYPERLTPDIDAVVLGMHASSENPELQKARVLGLKIYSYPEYLYEQTRG
                     KTRVVIGGSHGKTTITSMVMHVLQQAGFDFDYMVGAQLEGFDNMVGLNNQTNLAVFEG
                     DEYLTSPVDPRPKFHLYKPTIAVISGIAWDHFNVFPTIENYRDQFVGFISCIEPNGTL
                     VYCSEDDEVAGVVSSTKRSDLELIPYTTHPYRIEPNVGCQLVTEQACYPLKIFGRHNM
                     QNISAAKAVCLKLGVSESNFYRAISTFKGAAKRLQRLAEGANSTVFLDFAHSPSKVAA
                     TIGAVLEEYPNRKLVACLELHTFSSLNINFMSQYRNVFSGVAVPIVYFDPVTVEHKNL
                     PPLNINEVRDAFGQPIPDVCISASEMVEKLNGMNWENANLLLMTSGNFSGLNLNGLAL
                     QIAR"
     gene            1842..2321
                     /locus_tag="PK839_05360"
     CDS             1842..2321
                     /locus_tag="PK839_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72199.1"
                     /translation="MKIARVIVVSLALIMAVTTARAQDYKTGLGLRGGYPSGITVKHF
                     FGSKTAVDGILSFGWGGIGITGLYELHNRIPSAPGLNWYYGFGAHFATADANKNNPFA
                     NDRGDDVFLGVDGAIGLEFTFPDAPICISLDILPILNLIESPGIWFNAGLSVRYAFK"
     gene            2415..2486
                     /locus_tag="PK839_05365"
     tRNA            2415..2486
                     /locus_tag="PK839_05365"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2448..2450,aa:Glu,seq:ttc)
     gene            2515..2769
                     /gene="rpsT"
                     /locus_tag="PK839_05370"
     CDS             2515..2769
                     /gene="rpsT"
                     /locus_tag="PK839_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HOG72200.1"
                     /translation="MANHKSAEKRIRETETRRLHNRYYAKTTRNALKALRNTTNKAEA
                     IEMLPKVSSMLDKLAKKHIIHRNKAANLKSSIQKHVNALQ"
     gene            complement(2826..3632)
                     /locus_tag="PK839_05375"
     CDS             complement(2826..3632)
                     /locus_tag="PK839_05375"
                     /inference="COORDINATES: protein motif:HMM:NF025115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4163 domain-containing protein"
                     /protein_id="HOG72201.1"
                     /translation="MLKSRIIRLLSLAVLMGLVYFLFNTVRATYTPNLSVAPDSLVFT
                     RGCVEPDSSCFRILLKFPVVQGKRAARIQKMIAYDYLKLLSDSTKVKGVNQFKEVLTR
                     LTESYDSAYVDYLSLFPGSNGISWYLNADYDVLLNDGKVLTIQYRYEDYLGGAHGMYG
                     YHYQNIDLRKSRPLSLNDIVSNTKQFSELAEKLFKGRVGVDHLNGYWFEGGKFSLPRE
                     FGLSENGIVLHYNVYEIASFADGDLVFEIPYSSMGGMLSKRFRYLAEEGK"
     gene            4007..9553
                     /locus_tag="PK839_05380"
     CDS             4007..9553
                     /locus_tag="PK839_05380"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HOG72202.1"
                     /translation="MNSKSIKWLLTVGVIAIIILQVGCKGKQDVEISTDPLFGEYISA
                     YTSGMISAHSAITIKLTKPSSKFTFNGDEVHEGYFSFSPSVKGKAYWVDDQTLEYRPT
                     EVMRSGKVYSSSLALSKLFKGVEPKLKTFRFSFRVIPQSVSIELNGLIVRDAENPNSY
                     ELDGTVQTADVAESEKLNECIKASVNGMSIATKLDSTEVVNTYHLRLEGIERTVKKGE
                     VRISWNTSAIGGNSKGYEILEVPPLGSFKVISAKAIQTPEQSVTIIFSDLLDRNQNFE
                     GLVELEGISGLRYEVDRNMLRVYPPETIEGEKNLNISAGVLNAIGKRLNAEFSQNLLF
                     EDVNPALRTVGHGAILPTTQGLIFSFEAVNLKAVSVQIVKIYESNVPYYLQVNTMGGT
                     NELRRVGYPVLNKVIGLNQFGPIVPNKWTRYTLDLAQLFKADRGAIYQISITFNKKQL
                     LSSCGDDSDQTTPTSPFSPGDEEVARGYDGPGYYDDYYYYYDDDYNWRERDNPCNSAY
                     YSESRMINQIIISSDIGIIAKLGNDGGMLVAVTDLPSAKPLSNANVRISDYQNQTIAD
                     GNTNNDGFFEFKTTRRPFLVTASDGKQKGYLRVDNGTSNPLSNFDVSGNEVQHGLKGM
                     IYGERGVWRPGDTLHLCFILEDKGKTLPPNHPVVLELHDPKGLLVRRAVQPNNDAGIF
                     YFPTVTDPQAPTGPWSATVRVGGAIFQKTLKVESIKPNRLKINLELQGNNIPPSGMVN
                     ATLNAKWLHGAAAANLKAKYELTVSKANASFPEFSSYSFDDPGIDFPTQTQTLWDGQL
                     DDNGTARVYGSLNIPDSRPAAINVLFKGRVFEPGGDFSIDLFSKTFYPFSELVGVKPP
                     QPKMGTSWLETDTDQQFSVVTINNDGKPISCPNLELELYKLTWRWWWEQPETGEAGYV
                     NRNYDYLISSHKFGTVNGKATVKLRVDYPEWGRYYVKVTNKTTGNSCGTIVYFDWPYS
                     SGKSQANRPGGASILAISVDKPDCKVKESFKLTIPGAENSRALVSIENGYKVVKAFWV
                     DASSQANVVDIQTTPEMTPNVFVHVTLIQPHTNKTNDLPIRLYGITRINVSDPETTLQ
                     PVITMPDELKSDKPVEILIREKSGKKMNFTLAMVDEGLLDINRFKTPDPHSVFYAREA
                     IGVKTWDLFDEVIGAYGSTMERLISVGGDEELNPTQGEKIQRFKPVVRFFGPFVLEKG
                     KTEKISFNMGNYFGSVRIMVVAANDGAYGFAEKSCPVKNDLMVMATLPRVLGPQETVT
                     MPISIFTTSPAIKQVKIRVKTSSNLKVLNGNEQNLEFTKPGDLLAFFTLQVSEGTGPA
                     RVEVLAEGNGVKSTQVVDIEVRNPISIQVVSIDTLLPPNGKKSLQLSTFGIKGTNEAY
                     LEASVTPPLNLNKRLGYLIQYPHGCVEQTTSAVFPQLYLSQLVDLTADQQKKTSRFVS
                     EAIKKLAGFQNSDGSFRYWPSYVYGDDWSTSYVGHFLLEARSKGYDVPEGMLSRWTSY
                     QGKSARNYIPRPTNPFNSDLVQAYRLYTLALAAKPEVGAMNKLKEQELGLIAKMRLAG
                     AYALIGQKDVALAIVGSVPIKFSPYRELSYTYGSDIRDRGIVLETLILLGLDSKAYPI
                     ALEISKALSQNDWMSTQETAVALLALSRLSIDKEKGGELNLSYTLAKNSRVISSRRPV
                     AREQLEIRDGSNTLEIQNMTKNNVYASVVLKGVPAPGNETSYSSNLNIMARYRSASGD
                     ELNPYNLKKGMDFTCEVTVSNPGIRGELKELALTQIFPAGWEIQNTRFEGTENVSGST
                     FDYQDIRDDRVITYFSLKPNERRSFTIRLTATYSGRYYMPGPHCEAMYDNAISAGEKG
                     GWVTVE"
     gene            9561..11897
                     /gene="pbpC"
                     /locus_tag="PK839_05385"
     CDS             9561..11897
                     /gene="pbpC"
                     /locus_tag="PK839_05385"
                     /inference="COORDINATES: protein motif:HMM:TIGR02073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HOG72203.1"
                     /translation="MNFRRNPTGVWQVLTGITCLLVFLLLPPVRFKSETSTVLLDRNG
                     QLLSATVSDEGMWQFPASDTVPNKFATCITQFEDAYFRFHPGVNPFSMARAAFQNIKA
                     RKVVSGGSTITMQLVRLSRRGKPRTLGEKIVEMAMAVRTEISYSKPRILAMYSSNAPF
                     GGNVVGLDAAAWRYFGVSPQNLSWAEAATLAVLPNAPGLIFPGRNQHLLVEKRNKLLG
                     KLLKRGYIDDETYHLALLEPSPSLTGGLPHEATHLLTRCIHNGFKGKLVRSTIDRELQ
                     ENIMRVAQNHHGYLLANGIRNMAAMVVDNQTGEVMAYMGNVQSNNLSNSPMVDVISSK
                     RSYGSLLKPFLYGLMLSEGLLYPRQLVNDMPVAYAGFAPQNFSHTFEGVVPANEIISR
                     SLNVPSVNLLQQYGIEKFHSSLKQMGITSINKPAGHYGLSIILGGAEASLWELSGIYA
                     ALARQAQGIAAGNISLRYVLDSTTSSAQPFSCSGIGSGAAYLTLEALTQASRPGEDGT
                     WRNFVSSQKIAWKTGTSYGFRDAWAIGVTTCYTVAVWVGNADGEGRANLIGVATAAPV
                     MFDIFSLLPSSVWFSPPWFDLKPVVTCRQSGHQASPACTDTVTEYVPAVRTKTLPCPY
                     HQKVLVDKTHRYRLTQRCASQEEMVEVSWFVLPPAQEWYYSKLHADYRILPPFGKGCT
                     ENPDRLQSMALIYPYSGADIYIPVEAKGVKGQSIWKAAHRNPKATIYWHLNDRFIGKT
                     TGLHEVAVSPSPGVYTLTLVDDNGEMLQRRVRIAGEKK"
     gene            complement(12012..13646)
                     /gene="groL"
                     /locus_tag="PK839_05390"
     CDS             complement(12012..13646)
                     /gene="groL"
                     /locus_tag="PK839_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005941147.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HOG72204.1"
                     /translation="MSAKVLNFDIEARDKLKRGVDQLANAVKVTLGPKGRNVVMEKKF
                     GSQQVTKDGVSVAKEIELEDPFENVGAQMVKEVASKTSDTAGDGTTTATVLAQAIVNV
                     GLKNVTAGANPMDLKRGIDKAVAKVIENLKKQSVEVGDDYLKIEQVATVSANNDTEIG
                     KLIAEAMKKVKKEGVITVEEAKGIETTVEVVEGMQFDRGYISPYFITNPEKMETVLEN
                     PMILITDRKISTMKDLLPVLEPVAQAGRAMLIIAEDIEGEALATLVVNKLRGSLKIAA
                     VKAPGFGDRRKEMLEDIAILTGGVVISEEKGLRLDGAKMDMLGTAEKITIDKDNTTIV
                     NGGGKKDNINKRIAQIRTQIENTTSDYDREKLQERLAKLAGGVAVLYVGAATETEMKE
                     KKDRVDDALSATRAAVEEGIVPGGGVAYIRAIEALENMKGNNEDETTGIQIIKRAIEE
                     PLRQIMTNAGLEGSVIVQKVKEGKGDYGYNAQTNKFENLFQTGVIDPTKVTRVALENA
                     ASIAGLILTTECVITEKKEDKPVPMGNPGMGGMDMM"
     gene            complement(13687..13965)
                     /locus_tag="PK839_05395"
     CDS             complement(13687..13965)
                     /locus_tag="PK839_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HOG72205.1"
                     /translation="MSEVKIKPLADRVLVEPMEAEEKTASGIYIPDTAKEKPQKGTVI
                     AVGPGTKDVTMEVKKGDVVLYGKYAGTEISVDGKDYLMMKQSDILAII"
     gene            complement(14259..14606)
                     /gene="secG"
                     /locus_tag="PK839_05400"
     CDS             complement(14259..14606)
                     /gene="secG"
                     /locus_tag="PK839_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HOG72206.1"
                     /translation="MYTLFSVLIIIVSILLILIVLIQNPKGGGLASNFSGSNQVMGVR
                     KTADFLEKSTWTLAVALILFTLFATLAMPKKSAEEQKSVIESKVKNTAEPNALPTFPV
                     NPTEGNMGSDTTK"
     gene            complement(14629..15399)
                     /locus_tag="PK839_05405"
     CDS             complement(14629..15399)
                     /locus_tag="PK839_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72207.1"
                     /translation="MDNETLYGIMEARLKPTPETLNQLRETIDEFPYFQGCRVLILKS
                     LSQLGNDEFEAQLRQNAVLLPSRRYLYFTLYPSKPLEETEKIDGGNAEDKKISKPTPK
                     RGRPRKAPTDERKPSFILLDDEKQPAITIHVEASSNTTDTEILELAEGEGEVDSQANG
                     RGLNESEDLIAKFINNQPTISRSTTPGNKDTGENEDISISSVEEPEELASESLAQIYL
                     SQGYLEKAIRVFEKLSLKYPEKSSYFADEIRKIKESTK"
     gene            complement(15404..15919)
                     /locus_tag="PK839_05410"
     CDS             complement(15404..15919)
                     /locus_tag="PK839_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HOG72208.1"
                     /translation="MKRIIRIAIILLPLFVLIQPGCKVSYTFTGASISPETKTFSVQL
                     FQNLAGIVNPTLSSYLTEELKNRFITQTSLSMVDDFGDFAFSGQITQYNVSPISIQDN
                     EVAAQNRLTISVRVKFVNAKDPKLSFDKTFSQYENFDSNQNFVQVEAELVRLIVEKLV
                     DDIFNAAAANW"
     gene            complement(15923..17164)
                     /locus_tag="PK839_05415"
     CDS             complement(15923..17164)
                     /locus_tag="PK839_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOG72209.1"
                     /translation="MELQTIKQRFGIIGNAPGLNRAIDIARQVAPTDLSVLITGESGT
                     GKEVFPQIIHQFSARKHGNYIAVNCGAIPEGTIDSELFGHEKGSFTGATDSRQGYFEV
                     ANGGTIFLDEVAELPHSTQVRLLRVLETGEFIRVGSSKVIKTNVRVVAATNVNLYEAI
                     QEGKFREDLFYRLNTVPIYIPPLRERKEDIYLLFRKFAVDFSEKYRMPPITLDENARK
                     LLTDYSWPGNVRQLKNVTEQISIIEQSRVITADILQKYIPDNNVPRLPTLYSKKGATE
                     EPSFSSEREILYKILFDMRNDVNELKKLVNVIMQSGVTGAESSFADSAVLHNNFGPVN
                     PIITSPAQPTFEPMSFVKSNNEIQDTEEIMEESLSLEEKEVELIRKALDKHNGRRRQA
                     ASELGISERTLYRKIKEYKLD"
     gene            complement(17378..18835)
                     /locus_tag="PK839_05420"
     CDS             complement(17378..18835)
                     /locus_tag="PK839_05420"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HOG72210.1"
                     /translation="MKPKPATTFQRRLNLFDSTAIVVGSMIGSGIFLVSADMARNLGS
                     PGWLLVAWAITGILTVIAALSYGELAVMMPHAGGQYVYLREAYNPLAGFLFGWTLFLV
                     IQTGSIAAVSVAFSKFLGVIFPWFSDSNVLLQVGSLKITTIHLMSIFIILFLTWVNTR
                     GINIGKYVQDTFSYTKIAALLLFFIVGFFIARNDEAVRLNREIFWQGQQLVDGKPVPL
                     SGISLIIALGIAMVGSLFAADAWNNVTFASEEIKDPKRTIPLSMVLGVVIVIVLYMLV
                     NYIYIKVLPVRGSPDATTVMGQGMQYASNDRVGTAAMYGTFGESAAIIMAILIMISTF
                     GCVNGMILSGARVYYAMAKDGLFFKQVGILNSMGVPGVSLALQCAWACILCLSGTYSN
                     LLDYVIFSVLIFYVLSVVGVFILRVKKPDVARPYKTWGYPVTPAVYILACTFIMVILL
                     IYKPAYTWPGLIIVILGIPVYYLWKKPKFSRTKRT"
     gene            complement(19160..20644)
                     /locus_tag="PK839_05425"
     CDS             complement(19160..20644)
                     /locus_tag="PK839_05425"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOG72211.1"
                     /translation="MNRLSTLLFGILSAMPVMSCIAQQPMNRLTYPEVLQLAKEQSPQ
                     AILAKHRFRAAYWEYRTFVAEYRPTLSMNGTIPEFSRSLIRYQNPDGSYSYIEENANT
                     TSLGLSLSQNIGFTGGQIFVKSNLERTDLLGDSSSTSYLSVPVSIGFSQPLFAMNRLK
                     WNKRIEPIKYEEAKKSFIQSMEDISIEASRLFFNLAMAQQNLETARLNYSNTDTLYKI
                     AQGRYNIGTIAENELLQMELSFLNAGTQVNEAEVDLQLKKIQLKSFLGLNDQYDLEII
                     LPTAIPQFELIYDKVMMLARENNPQILSYERQLIEADRDVAQARSERGFNASLYATYG
                     LTQRALNVSDAYKDPQDQQNVRVGITIPILDWGLGKGKVKMAQSNREVIKTTVQQAMT
                     DFEQDIFLKVMQFNRQDDQVMIAEKADYISQNRYEVTKQRFLIGKIDVLDLNVAQSER
                     DQAKQKYISTLGNYWQFYYQMRRLTLFDLENNQPLQADFEMLLK"
     gene            complement(20666..22045)
                     /locus_tag="PK839_05430"
     CDS             complement(20666..22045)
                     /locus_tag="PK839_05430"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG72212.1"
                     /translation="MLRVDKLYKRYLHDINIAIEAIMANKLKSALTALGIIFGVAAVI
                     SMLAIGNGAQQEILEQMKMVGVNNIIVNSIFEAPDTQTSEEENGTKQKQKFSPGLTLK
                     DVEAIQQIIPSVKRISPEISLNSFVQYDGIRMPAKIMGVSNDYFSLFNLPLEDGVIFD
                     SYHEEHGVPVCVIGSNIKTKFFSTQNPIGQYIKFGSVWLRVIGVLAKSDIKVTGFESS
                     GINVYNDNIYVPVKSILLRYQNRALVNTRKISESGTSVFVSGGFVSYGGSTQSQAASN
                     YHQLDRIILQVNQTEDITSTSEVLSRMMLRRHSEVKDFEITVPELLLKQQQRTKDIFN
                     IVLGAIASISLIVGGIGIMNIMFASVMERIKEIGTRLAIGAKKADIIAQFLSEAVLIS
                     VSGGIIGVILGFVLSFLIRHFAGILTIVSPLSILVAFGVSAAVGVIFGLSPAKRAAEK
                     DPIESLRYE"
     gene            complement(22051..23526)
                     /locus_tag="PK839_05435"
     CDS             complement(22051..23526)
                     /locus_tag="PK839_05435"
                     /inference="COORDINATES: protein motif:HMM:NF012739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HOG72213.1"
                     /translation="MRKKVIIGLAVVLAIILAVVLFGSSDKDKAAPQFARVMQGKFEV
                     LVNVTGELKAQNSENIMAPTELRGRTFRISEVKIQDLIAEGTLVDSGDYVATLDRAAL
                     SNQLKSTEDELEKSLQAFLKVQLDTTLQLRELRNNLINLKFDMEEKQLVLEQSKYEPP
                     ATQRQAQINLDKAQRAYEQEVHNYTLKKEQAEASMKEVAINLQRQKRERQDMLDVLDK
                     FEIRAPKPGMLIYYREWNGQKRKVGSSVSPWDLIVATLPDLSVMNSSTYVNEIDISKI
                     KTGQPVRITVDAFPEKKFTGEVIEVANIGEQLPNTDAKVFEVTIRVNEYDPILRPSMT
                     TGNQIITNVYDSVTYIPLEALHANDSISYVYTRKGKMQVVVPGEMNENFIIIEKGLEA
                     NDEVYLSMPEKPEKFSLAGKELISVIKKKKEQKIQDEMRLREVQDSAMKSRSQAQKMM
                     FQMNGQQVRKNGSAPKINSELKGKGNSGSNQANPKPTAPKS"
     gene            24071..26374
                     /locus_tag="PK839_05440"
     CDS             24071..26374
                     /locus_tag="PK839_05440"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HOG72214.1"
                     /translation="MVTKISAMVMVATIGMGCINAQQPDYAQWVNPLIGTDNMGHTFP
                     GATVPFGSIQLSPDTDTVPYSLDGKSYNPEVYRYCAGYQYSDKTIVGFSHTHFSGTGH
                     SDLGDILVMPTVGQLQLNPGTSANPEKGYRSRYSHNNEYAKPGYYRVILDDYNIKAEL
                     TATTRVGVHRYTFPQSDSAHIIIDMVHGIYNYPEKNVWTFVRVESDTLVTGYRVTSGW
                     ARTRTVYFAIAFSKPFNTYGLVNDENLAYKGFWRKFNQNENFPENAGRRVKCHFDFTT
                     GSNEAIMLKVAISPVSTANAIENLKMEAPGWSFDNMAQHARDIWNHELGRIDVATLNQ
                     NDKVNFYTSLYHAYLGPTLYMDQDGSYMGLDQNIHKATGFENYTTFSLWDTHRALHPL
                     FNIIQPKRNADMIRSMLAHYSQSVHHMLPVWSHYANENWCMIGYHAVSVIADAAVKGN
                     TAFDLNEALNAAVATAENDWYDGVGYYMQYGYVPEDLSPNSVSKTLEYAYDDWCISQL
                     AAKANRPDLAARFYQRSRNYLNVFDKEIRFMRPRLSNGNFKKEFDVLSTHGQGFIEGN
                     SLNYSLYVPHDPESLASLLGGKEKLASYLDTLFTKYLPDEYFAQTEDITREGLIGTYV
                     HGNEPSHHVAYLYNWSAEPWKTQEKVRMICQTMYKPTPDGLSGNDDCGQMSAWYIFSA
                     LGFYPVAPASEFYSIGSPTVVNATINLENGKTFRISTLNQSPANIYVQNAILNGKPLT
                     GLKLSHSDIMSGGELQLVMGKKPIKRM"
     gene            26628..29126
                     /locus_tag="PK839_05445"
     CDS             26628..29126
                     /locus_tag="PK839_05445"
                     /inference="COORDINATES: protein motif:HMM:NF017077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG72215.1"
                     /translation="MIKPKVLTGFIIILLFSASSIIVTYQGFRELNLTRQEIGQPGQK
                     LAVINRLISGVYGGEADVRMFIITNDTGYLESFEERKKKVTSVLNSLDGLMAETPGQQ
                     QNVKQIKALLSRKWAIVNELLALRKTYTDDRFYEEALKQIERAGTESRIRASVAKTTT
                     VTTTLHDTVVTLENKDENLFGKIKRFFVGPERVDTVSRSVNIETRLDTIDKGQYVSDS
                     IINNLVKLINGIRADQRKYQFDLGVKEAELLEQDRELMKKIESIVSSLERQELSSSIR
                     QSVLAQDLVNRSIIKILALGSFTFILLLVLLAVIFRDISRSNQYRTQLLEAKQYAERL
                     LRVKEQFLANISHEIRTPLSAVIGLTHQLGKTDLNARQQDYVSTLSSSADHLLSVIND
                     ILDYSKLESGQVRFETAMFDPVNVLESVTQTFNPKAEEKQLLVHTNIDPAVPHVLWGD
                     SFRLKQALMNLLSNAIKFTESGSIIVSLGVVRLTPEYVKLQFTVADTGVGIPPEQQQA
                     IFEEFTQADSSISRKYGGTGLGLTLVKKIVELQGGEISLESKINEGTIVKFSIPYALT
                     GVETKEETLERSSFAIPNNTAILVIDDDEVNLLIVTEMATTLGLQVDTLSKPEALYET
                     LSSKKYSAILTDIQMPGLNGYEVVKLVEQFNENIPVVAITANSMVDSPEHFTSHGFAG
                     YLIKPFVETDLQKVLSPLIGIPKPVKQLQVSTKSRRSSPAQIDLSDLYRFTGGDKNSL
                     RLILTSFLDNTYRNADELNRYIREKNLKQASEVAHRMKSAFSQLKIYHISSLLQKIET
                     LKPDKYRAANIYLDYLNKQIKPVVKEIRELLEKL"
     gene            complement(29172..32480)
                     /locus_tag="PK839_05450"
     CDS             complement(29172..32480)
                     /locus_tag="PK839_05450"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOG72216.1"
                     /translation="MRRYFLFALLGITVYTATGQPYNRLGNPLVTNFTPEDYDATEQT
                     WCITQDDRGVMYFGTYDKGILEYDGKTWRKIQVPDSKPVNSMIKGDDGVIYVGTSGDF
                     GFLEPTADGSQRFVSLSGSINDTIKQSLSHIYKTYYFKGNVLFCSCSYIFVYDKQGKL
                     TPVYLGYQPEYANFLTFVVNDKVYIGSYLKGLRMLNSDWTLSTAPGGSSFERINIYSI
                     VSLSPSELLLVTDSGFFVYNQKTGSSKKMEKQNNFIESMLSQSVIPYHAIKLHNGNIG
                     VGTVQSDWLSFFELNADSSSPSTIVNPQTGLQAAQITYLYQNGESPVWATLFDGGISR
                     IELHSPIRRFSTESGINELILDIIQFNGTLYVATINGVYYQTAAANGMSVFKPVEGIN
                     GVVWSLLKFTPPGGSPMLLAGSYVDGVFEIKGNRAISISKPLYRSFPNVQHQCLTLYQ
                     SVLHPNMLYVGGISRLTYIEWSKGEWVKTQNIALDEIRSEVWSIGEDARGNLWLGTNL
                     KGVYMIDPHEKVYNFNSSSDFSGFKLLRIYPDGDSLYVLTPKGIFYYSYSDSTFRKCG
                     LLGKNSLENGFSRLLKVGNEIVAACYNDSTGKYWVCTMAMDSSGEWVTNDKPFKRLPN
                     KGADALYVDDGGVLWIGIGKELFSFNTRVKRSYNAPYHALVRSVYAKDSLLFNGSFSE
                     VDQDGRKITLLQQPQSKIPQLKYRYNGLVFSFSSPYFEEEAELLYSHYLEGSDETTWS
                     AWDGRTEVTYTNLSEGKYTFHVKAVNIFGEESIPATFTFVIHPPWYRTLLAYFCYFIF
                     FIGLVWGLVRWNTRRLIAEKERLEKIVEERTAEVVAQKEEIERQKEKISIQNEEITSS
                     IQYASRIQSALLTPSELVSRIFPEHFILYLPRDIVSGDFYYVTQVESKKICIVADCTG
                     HGVPGGFMSMLGISFITQILSEVKELQAADILNRLRQLVISTLHQTSDVGGSKDGMDI
                     TIYIVDEQSMTMQFAGANNPLILIRNGEVTQVKGDKMPIGIHLRGEVPFTNNVIEIQK
                     GDVMYTFSDGYVDQFGGESGRKFMIKDFRELLLKIHTYPMAEQRQILLDTLISWQGDT
                     PRIDDVVVMGVRIV"
     gene            32896..33669
                     /locus_tag="PK839_05455"
     CDS             32896..33669
                     /locus_tag="PK839_05455"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HOG72217.1"
                     /translation="MRISRPAVLIIVFASLMQVSCNRKAQLLYLNNLDTGLLTSTQPA
                     PYQLRPGDVLSVQVITQDPEISRLFNIVSSGTSTSYTAYSNDASLYINGFSVNDSGMV
                     RLPVVGTMPVAGLTVRQAQDSIQLKYERYLKDALVVVKLLSFKVTVLGEVRRPGTYTN
                     YRDNLNIFEAIGLAGDFSDYGERTNVLVLRQTPEGVKTFRLNLQDKNLLTAEGFYLLP
                     NDTVIVEPRNAKVMALNTPNISIFLSIITTALLLLNYVK"
     gene            33728..36208
                     /locus_tag="PK839_05460"
     CDS             33728..36208
                     /locus_tag="PK839_05460"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HOG72218.1"
                     /translation="MKGDGMEDNMNLKSNPQQQDLPEEEQLDVKKLLFRVLANWYWYA
                     ITLFISLFIAYLINRYSEQSFSVSSSVIVRDDDQTKSYVGAEQLIQSLRLVKNTKSVQ
                     NEIGILQSYSLARQTVEELEDFNVTYVAVGRRGIKEQKLYTGSPFKVVIDSGATNMQG
                     HPVRVNILNSREYRLEIDEGMGISRTMRFGEPFTNESFNFTVFLRDPLNYDPKVAGRK
                     YYFTINDPNTVALSYKSKVTVTLNDKKGSILTLNSTGYVAQQEADYLNKLMEVYIRRG
                     LEEKNQIAENTIRFIDRQLEDMADSLRSAELHLQDFRTSNRVLDITGEGNLLLEQIKT
                     IQNEQSSMELKARYYAYLSDYLEQRKDFNTLVAPSVMGVDDPQLNALLSEISKAYIDR
                     EELKTTVREGSPGLEQLNIRIENLRSSLAEKVKSLIESNAIAQQELNSRLSRVDAEIK
                     RLPVNERMLIGFERDFSLIDKMYTYLQEKRAEAAIARASNIPDNKVLDYAIAQNASLI
                     KPKRSTNYLIALLVGLAIPFIIMLLYDFFNDKIVDLKEISKRTRVPVIGTLGHNRYQT
                     ELPVLEKPKSTLTESFRGLRTNLQYLMREPESRVVSVSSTVVGEGKTFIAVNLAAAIA
                     VTGKRVLLLGLDLRKPRLHRLMGYSGDIGVSTYLIGQNSAGDIVFPSQHENLYFAPSG
                     PIPPNPAELLGTARMAELVAWARQNFDFIVVDSPPVAIVSDIFIIARFTDANLFTIRY
                     GYSSKEVLNLINNIYRHHEVRNLAMVINDFEPRRGYSYGYGYAYSYGYSYGYGYRYGY
                     GPYTNSDGYYSDEDEPKPTWKERIKRLF"
     gene            36439..>36805
                     /locus_tag="PK839_05465"
     CDS             36439..>36805
                     /locus_tag="PK839_05465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72219.1"
                     /translation="MYYIDLRNKFINLGCFNINQIYAFDPDFNRNNLTYWQKKGWLIY
                     LRNGYYAFPEFKQEINSQYFLANKIYRPSYVSLQSALSYYEIIPESVVQITNVTTLKT
                     KSFENDFGQFYYKSIKSDFY"
BASE COUNT         9955 a         8850 c         8398 g         9602 t
ORIGIN      
        1 gccagtttgt agtagatttt tgaaaatttt gcggaagtag ctcagttggt agagcataac
       61 cttgccaagg ttagggtcgc gagttcgagt ctcgtcttcc gctcagttct ttattaaagt
      121 tcaaagtttt gcactttgaa ctttttaagt ttaataccga aaatgcccgg gtggtggaat
      181 tggtagacac gcaggactta aaatcctgta gccattgcgg ctgtgcgggt tcaattcccg
      241 ccccgggtac gttaaagcct cgcacttgcg gggctttaat atttatcaga atcagcttac
      301 acctacataa agcagccact caccaaccta agattactac ttttcaaaat ataaattcta
      361 catttggctc tatcaaccac cacacattta aaacatgcga gtacacttca ttgccattgg
      421 gggaagcgcc atgcacaacc tggcaattgc gttgaaacaa aagggatacg aggttaccgg
      481 ttcggatgat gaatttttcg aaccttcact ctccaggtta aaacagcatg ggctgctccc
      541 gtcagagatt ggctggtatc cggaaaggtt aacgcccgat attgatgccg tcgtgctggg
      601 catgcacgcc agttcagaaa atcctgagtt gcaaaaagcc cgagtgcttg gtttgaaaat
      661 atactcctat ccggagtacc tctatgagca aaccagggga aaaaccaggg tagttattgg
      721 tggcagccat ggaaaaacca ccatcacctc gatggtaatg catgtgttgc agcaggctgg
      781 ctttgatttc gattatatgg tgggagccca gctggaggga tttgataata tggttggact
      841 taacaaccaa accaacctgg cagtatttga gggcgatgaa tatttgacct cacctgtgga
      901 cccacgcccg aaattccatc tttacaagcc aaccattgca gttatttcgg gtattgcatg
      961 ggaccatttt aacgtcttcc ctaccattga aaattaccgt gatcaatttg tgggtttcat
     1021 cagctgtatt gagccaaacg gaacgctagt ttactgtagc gaggatgatg aggtagcagg
     1081 ggtagtaagc tccacaaaac gttcagacct ggagttgatt ccgtacacaa cacatcctta
     1141 ccgaatagaa cccaacgttg gctgccagct ggttaccgag caggcatgct accccttaaa
     1201 aatttttggt cgccataaca tgcagaacat tagcgctgct aaggctgtat gcttaaaact
     1261 tggagttagc gaatcgaatt tttaccgggc aatctccacc ttcaagggag ctgcaaaaag
     1321 actgcaacgg ctagccgaag gagcaaactc cacagttttt ctcgattttg cccactcgcc
     1381 atcaaaagtt gccgccacta ttggcgctgt tctggaggaa taccccaatc gtaagctggt
     1441 tgcctgcctg gagctgcaca cctttagcag cttgaacata aattttatga gccagtaccg
     1501 taatgttttc tcaggtgttg cggtgccgat agtttacttt gacccggtta cagtggaaca
     1561 caaaaattta cctccattaa acattaacga ggtgcgggat gcttttggac agcctatacc
     1621 agatgtgtgc atttcggcaa gcgaaatggt tgaaaaactt aacggcatga attgggaaaa
     1681 tgccaacctg ttgctgatga cttcggggaa cttttcgggt ttaaacctta acgggttggc
     1741 tttacaaata gcacgatgat ttaaactttg ttaaagatat cctgttatag gatattgttc
     1801 ctacttttac aacacatatt aactaaccaa aataaaagca catgaaaata gcaagagtaa
     1861 tagtggtatc gctagcgctt ataatggcgg taacaaccgc aagagcgcag gactacaaaa
     1921 ccggtttggg attacgaggc ggataccctt cgggtatcac ggttaaacat ttttttggca
     1981 gtaaaacagc tgttgacggc atcctttcgt ttggctgggg tggaattggg attaccggcc
     2041 tttacgaatt acataaccgg attcccagtg ctccaggact caactggtac tacgggtttg
     2101 gcgctcactt tgccacggct gatgccaaca agaataatcc atttgccaac gacaggggcg
     2161 atgatgtgtt tctaggcgtt gatggggcaa ttggattgga gtttactttc cccgatgctc
     2221 ccatttgcat cagcttggat attctgccta tcctgaacct catagaatcg cccggtattt
     2281 ggtttaatgc aggcctttcg gttcgctatg cttttaagta aaaacagcat aaattttttc
     2341 ccatttattt gtagaaaata aaaattcact tacctttgca cccgccttgg taggcaatat
     2401 gctacttaga gtaaggcccg ttcgtctagg ggttaggacg tcaggttttc atcctggtaa
     2461 cagcggttcg attccgctac gggctacgaa taacgaacct atttataaac ggatatggca
     2521 aatcacaaat cagcagaaaa acgaatcagg gaaaccgaaa cccgaaggct tcacaaccgc
     2581 tactatgcca aaactacacg taacgccctg aaagctttac gtaacaccac taacaaggca
     2641 gaagcaatag agatgcttcc caaggtttca tcaatgctcg acaaacttgc caaaaagcat
     2701 attattcata ggaataaagc tgcaaatctt aagagttcca tacaaaagca tgtaaatgcc
     2761 ctgcagtaag aaaggcttaa ttttccatga actgcacttt gcgcctccca caatcgaggc
     2821 gttttttatt taccttcttc ggctaggtac ctgaatcgtt ttgaaagcat cccccccatg
     2881 ctggagtatg gaatttcaaa gaccaaatcc ccatcggcaa agctggctat ttcatacacg
     2941 ttgtagtgaa gcactattcc attttcgcta agtccaaact ccctgggtag gctgaatttt
     3001 cctccttcaa accagtagcc attcaaatga tcaaccccta ccctaccctt aaagagtttt
     3061 tcggccaatt cggaaaattg cttggtgttg cttacaatat cattaaggct gagcggtctg
     3121 gacttacgca ggtctatgtt ttggtaatga tagccataca ttccatgtgc tcccccaagg
     3181 taatcttcgt agcggtactg gatggtcaac acttttccat cattcaacag gacgtcgtaa
     3241 tcggcattta aataccacga aatgccattg ctgccaggaa ataacgatag gtaatcaaca
     3301 taagcactat catagctttc ggttaaccgg gtaagaactt ccttaaactg gttaactccc
     3361 tttacctttg tgctgtccga taaaagtttc aggtagtcgt aggctatcat cttttgaatt
     3421 cgggctgccc ttttcccctg aacaaccgga aacttaagga gtatcctaaa acaggaagaa
     3481 tcgggctcta cgcatccacg tgtaaacaca agagaatctg gagccaccga taagttgggt
     3541 gtataggttg cccttacggt gttaaataga aaataaacca accccataag aactgctaac
     3601 gatagaagac gtattattct ggacttcagc atattactat tttcccttta aatattgaac
     3661 cagctccagg cgagctatta aacctatttc tttactgctc aaatatagct cgataatttc
     3721 gaaaaatctt atgctaggtc tgccccattc atcacccgtc gtaaaccttc aaatgccgct
     3781 cgcctcccaa ccgattgaac tacatataaa atgcttaaat acagatgtac gacccataac
     3841 cttgcggtat tcgggttcta aaagcgcatt gtttttaaag ttcctttacc ttaagaaagt
     3901 agatggatgt ttgtgtgtta ccaatatttg tgggcatttc aatattaatg cggtaaactc
     3961 aaaatcttga gtaattttgg gtagtagtat aaatcatcct tcaagcatga acagtaaatc
     4021 aatcaaatgg cttttaactg ttggggttat agccataatc atccttcagg ttggatgtaa
     4081 gggcaaacag gatgttgaaa tttcgactga tccgctattt ggagagtaca tttcagcata
     4141 tacctcggga atgatttcgg cacactccgc catcacaatt aagcttacaa agccaagcag
     4201 caagtttact ttcaatggcg acgaggtaca cgaaggctat ttcagctttt caccatcagt
     4261 aaagggtaaa gcctactggg ttgatgacca aactctagag tatagaccga cagaggtaat
     4321 gcgttcgggc aaagtttact cctcttccct ggcattaagc aagcttttta aaggtgtaga
     4381 accaaagcta aaaacgtttc gctttagctt cagggtaatt ccacaatctg ttagcattga
     4441 gctgaatggt ttgattgtta gggatgcaga aaatccaaac agctatgagc tcgatggcac
     4501 ggttcaaaca gctgatgttg cagagtccga gaaactgaac gagtgcataa aggcaagcgt
     4561 caacggaatg agtattgcta caaaacttga ttccaccgaa gtcgtcaaca cctaccattt
     4621 gcgtctggag ggaattgaac gaaccgtaaa aaaaggagag gtaaggatat catggaacac
     4681 ctcggcaata ggaggaaact caaaggggta tgaaatcctt gaggtgccac ccttgggtag
     4741 ctttaaagtg attagcgcaa aagccattca aacgccggag caatccgtca ccattatttt
     4801 ttccgaccta ctggacagaa accagaactt tgaggggttg gttgagcttg aaggaatttc
     4861 ggggctgagg tacgaagttg accgaaacat gctccgggtt tacccacccg aaactattga
     4921 aggcgagaaa aatcttaaca ttagtgctgg tgtgctaaat gcaattggta aaaggcttaa
     4981 tgctgagttt tctcaaaacc tactgtttga ggacgttaat cctgcacttc gcactgtggg
     5041 gcatggagca attctgccta caacccaggg gctgattttc tctttcgaag cagttaacct
     5101 taaagcggtt agcgttcaga tagtgaagat atacgaaagc aatgtgccct actaccttca
     5161 ggtaaatacc atgggaggga ctaacgagct aagacgtgtg ggttaccctg tgctcaacaa
     5221 ggtcatcggg ttgaaccagt ttggcccaat tgttccgaat aaatggacca ggtacacgct
     5281 ggatctcgca caacttttca aagccgacag gggcgcaatt taccaaatta gcattacatt
     5341 caataaaaag caacttcttt cttcctgcgg ggatgactcc gaccaaacca ctcctacatc
     5401 tccttttagc cccggcgatg aagaagttgc acggggttac gatggacccg gttactacga
     5461 cgactactac tattactacg acgatgatta taactggaga gagagagaca acccctgcaa
     5521 ctcggcctac tactcagaat cgagaatgat taaccagata ataatatcat cagatatcgg
     5581 tattattgca aagctaggta atgacggtgg aatgctggtt gctgttaccg acttgccttc
     5641 agcaaaaccg ttgtctaacg ccaacgtaag aatttccgac taccagaacc aaaccatagc
     5701 cgatggtaac accaacaacg acggattttt tgagtttaaa acaacacgaa ggccattttt
     5761 ggtaaccgca agtgatggca agcaaaaagg atacttgcgt gtagataacg ggacatcgaa
     5821 cccgctaagc aacttcgacg ttagcgggaa tgaggttcaa catggcttaa agggaatgat
     5881 atacggtgaa aggggtgtat ggcgccccgg cgataccctt catctctgct ttattcttga
     5941 ggacaaggga aaaacacttc cgccgaacca cccggtagta cttgagctgc acgatccgaa
     6001 gggtttgctg gtcaggagag ctgttcagcc caacaacgat gcagggattt tctactttcc
     6061 aacagtaacc gatcctcaag ctcctaccgg accatggagc gcaacagtta gggtgggcgg
     6121 agccattttt caaaaaacct taaaggttga aagtataaaa ccaaaccgtc ttaaaataaa
     6181 ccttgaactt cagggcaaca acataccccc gtcgggcatg gtcaacgcta cccttaacgc
     6241 caaatggctg catggcgcag ctgcggcaaa tctaaaagct aaatacgagt taactgtaag
     6301 caaggcaaat gcttcgtttc ccgaattcag cagctactca tttgacgatc cgggaattga
     6361 ttttccaacg caaacacaaa ccttgtggga tggacaactc gacgataatg gcaccgccag
     6421 agtctatgga tcactaaaca tacccgatag ccgcccagcc gctattaatg tgctttttaa
     6481 gggaagagtt tttgaaccgg gtggtgattt cagcatcgat ctgttttcta aaacatttta
     6541 cccgtttagc gagcttgtgg gagttaagcc accacagcct aaaatgggaa ccagctggct
     6601 cgaaaccgac accgaccagc aattctcggt agttaccatc aacaacgatg gcaagccgat
     6661 aagctgtccg aacctggagc tggaactcta caagctaacg tggcgatggt ggtgggaaca
     6721 gcccgagaca ggcgaggccg gctacgtgaa ccggaactac gactacctta tatcgagcca
     6781 taaatttgga acagtcaacg gcaaggctac cgttaagctt agggttgatt atcccgaatg
     6841 ggggcgctac tacgttaagg ttaccaataa aacaacgggg aacagctgcg gaacaatagt
     6901 atacttcgac tggccataca gttcaggaaa atctcaggct aatcgtccgg gtggtgccag
     6961 tattctggct atctcggttg ataagcccga ttgtaaagtt aaagaaagct tcaaacttac
     7021 cattcctggc gccgaaaatt cgagagctct tgttagcatt gagaatgggt acaaggtggt
     7081 taaagcattc tgggttgatg cctcaagcca ggccaacgtg gttgacatcc aaaccacacc
     7141 ggagatgacc ccaaatgtgt tcgtccatgt taccctgata cagccacata ccaataagac
     7201 caacgacttg cccatccgtc tctatggaat cacccggatt aacgtttctg accccgaaac
     7261 aacgctgcaa ccagtaatca ctatgcccga tgagttaaaa tcggataaac ccgtcgaaat
     7321 tctcatcagg gagaagagcg ggaaaaaaat gaactttacg cttgcgatgg ttgacgaggg
     7381 gttgctcgac atcaaccgct tcaaaacccc cgatccccac tcggtatttt acgcccggga
     7441 ggccataggg gtgaaaacat gggatttgtt cgacgaagtc ataggtgctt acggaagcac
     7501 aatggaaagg ttgatttctg taggtggcga cgaggagcta aatcctaccc aaggagaaaa
     7561 aatccagcgc ttcaaaccgg ttgtccgatt ctttggcccc tttgtgctcg aaaagggtaa
     7621 aactgaaaaa ataagcttca acatgggaaa ttattttggc agcgtaagga taatggttgt
     7681 tgcagccaac gatggagcct atggttttgc tgaaaaaagt tgtccggtaa aaaatgacct
     7741 gatggtaatg gccacccttc cccgggtact gggacctcag gaaacagtaa ccatgccaat
     7801 cagcatcttt accacatcgc ccgccattaa gcaggtgaag attagggtaa agacctcttc
     7861 aaacctgaaa gttctgaatg gtaatgagca aaacctagaa tttacaaaac ctggcgacct
     7921 gttagcattc ttcacattac aagtttccga aggcaccggt ccggcaaggg ttgaagtgct
     7981 tgccgaaggc aacggggtaa aatcaaccca ggtagttgac attgaagtac gtaatcccat
     8041 ttcaattcaa gttgtaagca ttgataccct cctaccgcca aacggcaaga aaagtttgca
     8101 gcttagcact tttggcataa aaggcacaaa tgaagcttac ctcgaggcat cggttacacc
     8161 acctctaaac ctaaacaaaa ggttgggcta ccttatccag tatccccatg gctgcgtgga
     8221 gcaaacaacc tcagcagtat tcccccaact ttaccttagc caactggttg atctgacagc
     8281 cgaccagcag aagaaaacct cacgattcgt ttctgaggct attaaaaagc tggcagggtt
     8341 tcagaatagc gatgggagct ttcgctactg gccatcctat gtttatggtg atgactggag
     8401 taccagctat gtaggccatt ttcttcttga ggctcggagc aaagggtatg atgttccaga
     8461 gggcatgcta tcgagatgga ccagctacca gggaaaatcg gcaagaaatt acattccccg
     8521 gcctaccaac ccgtttaaca gcgatttggt tcaggcatac cggttgtaca cccttgctct
     8581 cgcagcaaaa cccgaagttg gcgctatgaa taagcttaag gagcaggaac ttggccttat
     8641 tgcaaaaatg aggctggccg gcgcctatgc ccttattggc caaaaggatg tagcgttggc
     8701 catagtagga agtgttccca ttaaattcag cccatacagg gagctaagct acacttatgg
     8761 ctcggacatt agggacaggg gaatagttct ggaaacctta attttgctgg gtttggattc
     8821 aaaagcttat ccgattgccc tggagattag caaggctctc tcgcaaaacg actggatgag
     8881 cactcaggaa actgcagtag cattacttgc cctctccagg ttatccattg acaaggaaaa
     8941 gggtggcgag ctaaatctat cctatactct tgccaaaaat tccagggtga tttcaagtcg
     9001 caggcctgtt gcccgcgagc agttggaaat acgcgatggc tctaacactc ttgaaattca
     9061 aaatatgact aaaaacaacg tatatgccag cgttgttcta aaaggagtac ctgcaccagg
     9121 caatgagaca tcgtacagca gcaacctcaa catcatggct cggtaccgtt cagcttcagg
     9181 tgatgagctg aatccctata acctcaaaaa gggaatggat ttcacctgcg aggtaactgt
     9241 ttcgaacccc ggtatccgtg gggaacttaa ggagcttgcc cttacacaaa ttttccctgc
     9301 aggatgggaa attcaaaaca cccgcttcga ggggacggaa aatgtttcgg ggtcaacttt
     9361 tgactatcag gatattcggg acgaccgggt tatcacctac ttcagcctta agcctaatga
     9421 aagaaggagt ttcaccataa ggctaacagc cacctattcc ggcaggtact acatgccggg
     9481 tccacactgc gaggcaatgt atgataacgc catttctgcc ggtgaaaaag gagggtgggt
     9541 aaccgtggag tagcaagcca atgaatttta gaaggaatcc tacgggtgtt tggcaggtgc
     9601 ttactggtat aacgtgcctg ctggttttct tgttacttcc ccctgtaagg ttcaagagcg
     9661 aaacctcaac cgtgctactc gaccgtaacg ggcagctgct ttcggctact gtttccgatg
     9721 aaggaatgtg gcagtttcct gcctccgata cggttcccaa taagtttgca acttgcatta
     9781 cccagtttga ggatgcctac ttccggtttc accctggcgt aaaccctttc tcaatggctc
     9841 gtgcagcctt ccaaaatata aaagcccgaa aagtagtaag cggtggcagc accataacca
     9901 tgcagctggt tcgattgtcg cgacgaggaa aaccaagaac cttaggtgaa aagattgttg
     9961 aaatggcgat ggctgtccga acagaaattt cgtattcaaa acctcgaata ctcgccatgt
    10021 attcatccaa cgcacccttt ggaggaaatg tggttgggct cgatgcagca gcttggcggt
    10081 actttggagt aagcccgcaa aatctaagtt gggccgaagc tgctacgctg gcagtgctac
    10141 ccaatgcccc cgggttgatt ttccccgggc gtaaccagca cttgcttgtt gaaaaaagga
    10201 ataagctgct gggtaaactc ctgaaaaggg gctacattga cgatgagacc taccatcttg
    10261 ccctgctgga accttcccca agcttaaccg gggggctgcc ccatgaagca acccacttgc
    10321 ttacccgctg catccacaac gggtttaagg gtaaactcgt acggtcaacc attgatagag
    10381 aactccagga aaacatcatg agggttgcgc aaaaccatca tggatacctc ctggcaaatg
    10441 gaattcgaaa tatggctgcc atggtagtgg acaaccaaac gggtgaggtt atggcctaca
    10501 tgggcaatgt tcaatccaac aatctatcca actcaccgat ggttgatgtg attagttcaa
    10561 aacgaagcta cggaagcctc ctcaagcctt ttctctatgg gctaatgctt agtgagggat
    10621 tactctaccc ccgccagctg gttaacgata tgcccgtagc ctatgcagga tttgcgcctc
    10681 aaaattttag ccacaccttt gaaggcgttg tgccagccaa cgaaatcatt tcccgatcac
    10741 taaacgtgcc ttctgttaac cttttgcagc agtatggaat tgaaaaattt cacagcagcc
    10801 ttaaacagat ggggataacc agcatcaaca agcctgcagg tcactatggt ctttccatca
    10861 tacttggggg cgctgaggct agcctttggg agctatcggg catatatgct gctcttgccc
    10921 ggcaggcaca ggggattgct gcaggaaata ttagcttacg ctatgttctg gatagcacaa
    10981 catcctccgc acagcccttc agctgcagcg gaattggttc cggtgctgcc taccttacac
    11041 ttgaagcact aacgcaggct agccgccccg gtgaggatgg aacctggcga aactttgttt
    11101 cgtcgcaaaa gattgcatgg aaaacaggaa ccagctatgg gtttcgtgat gcttgggcta
    11161 ttggagtaac cacttgctac acggtagcag tgtgggtggg aaatgccgat ggcgagggga
    11221 gagcaaatct tattggcgta gccacagctg cccctgtaat gtttgacatt ttttcgctgc
    11281 tcccttcatc ggtgtggttt tctccgccat ggttcgactt aaaacctgta gtaacctgca
    11341 ggcaaagcgg acaccaggcc tcaccagcct gtaccgatac agttaccgag tatgttccag
    11401 ctgttaggac aaaaacgctg ccttgcccct accatcaaaa ggtgctggtt gataagactc
    11461 ataggtatag gctaacccaa cgctgcgcaa gccaggagga gatggttgag gttagctggt
    11521 ttgttctgcc gccagcccag gagtggtact actccaaact acatgcagac tataggattt
    11581 taccaccatt tgggaagggg tgcacggaga atcccgacag gctgcaatca atggcgttaa
    11641 tttaccccta ttccggcgca gacatctata tcccggtaga agcaaaaggc gttaagggtc
    11701 aaagcatttg gaaggctgcc caccgaaacc ccaaggcaac tatctactgg catctgaacg
    11761 atagatttat cggaaaaact acaggtttgc acgaagtagc cgtttcgcct tccccgggag
    11821 tttatacgct cacgcttgtt gacgacaacg gtgaaatgct gcagcgaagg gtgaggatag
    11881 ccggcgaaaa gaagtgagta accacaacta gcaccaactc gtaggggtta cacttttccg
    11941 gtaggtaagg tagatgaaaa aaaacggagg atattcaatc ctccgttttg taggctatgc
    12001 atggttgaat gttacatcat gtccattccg cccattccgg ggttgcccat tgggacaggc
    12061 ttatcttcct tcttctcggt gataacacac tcggtggtaa gaattagccc ggcaatagaa
    12121 gctgcatttt ccagagctac acgggtaacc ttggttgggt cgataactcc ggtttggaat
    12181 aggttttcaa acttgttggt ttgagcgttg tagccatagt cgcccttacc ctccttaacc
    12241 ttctgaacaa ttaccgaacc ttcaagaccg gcattggtca ttatctggcg taggggttct
    12301 tcgatagccc ttttaataat ctggataccg gtggtttcat cttcgttatt tcccttcatg
    12361 ttttcaagag cttcgatagc cctgatgtag gcaacacctc caccgggtac tataccctct
    12421 tcaacagctg cgcgagtagc gcttagggca tcgtcaaccc tatccttctt ctccttcatt
    12481 tcggtttcgg tggcagcgcc aacgtagaga acggctaccc cgcctgccag cttggcaaga
    12541 cgctcctgaa gcttctcacg atcgtagtcg gaggtggtgt tttcaatctg ggtgcgaatt
    12601 tgggcaatac gcttgttgat gttgtccttt ttgcccccgc cattcacaat agttgtgttg
    12661 tccttatcaa ttgtaatttt ttcggcggtt cccagcatgt ccatcttagc gccatcaagc
    12721 cgtaggcctt tttcctcgct aatcaccaca ccaccggtta ggattgcaat atcctcgagc
    12781 atctccttac ggcgatcgcc aaatccgggt gcctttactg cagcaatttt gagcgaaccg
    12841 cgaagcttat ttacgaccag tgttgcaagt gcttcgcctt caatatcctc agcaatgatg
    12901 agcattgcac gtcctgcctg agcaaccggt tcaagaacgg gtagcaggtc cttcatggtc
    12961 gaaatcttac ggtcggtgat aagaatcatg gggttctcca gaaccgtttc catcttctcg
    13021 gggttggtaa tgaagtaggg tgaaatgtat cccctgtcga actgcatacc ctcaaccacc
    13081 tcaacagtag tttcgatacc cttagcctcc tcaacggtga ttacgccttc ctttttaacc
    13141 tttttcatgg cttcggcaat cagcttacca atttcggtgt cgttgtttgc cgaaacagtg
    13201 gccacctgct caattttaag gtagtcgtcg cctacctcaa cagattgctt tttaagattc
    13261 tcaatcacct tggcaacagc cttgtcaata ccacgcttca aatccatagg attggctcca
    13321 gcggtaacat tttttaatcc tacgttgaca attgcctggg ccaaaaccgt tgcagttgtt
    13381 gttccgtcgc cggctgtgtc ggaggtttta gatgccacct ccttaaccat ctgggcacca
    13441 acattttcaa atgggtcttc cagctcaatc tccttggcaa ccgaaacgcc atccttggtt
    13501 acctgctgtg agccaaattt cttttccatt accacgttgc gtcccttagg tcctagggtt
    13561 acttttacgg cattggccag ctggtcaacg cctcttttaa gcttatcgcg tgcttcgata
    13621 tcaaaattta agacttttgc tgacatagtt atgcttgatt tagttgttta acaatgtaga
    13681 aaccgattaa ataatagcca gaatgtcgga ttgtttcatc atcaggtaat ccttaccatc
    13741 aacggagatt tccgtgccgg catacttgcc atatagcact acatcgcctt tttttacctc
    13801 catggtaaca tcttttgtac cgggacctac ggctattact gttcccttct ggggtttctc
    13861 ctttgccgta tcgggaatgt aaattccact tgctgttttt tcttctgctt ccatgggctc
    13921 aacgagcacc ctatcggcta acggtttaat ttttacttct gacatagcac tatattttaa
    13981 tgttagtatt ttgattttta cgagagggtg tgcatcataa tacatgccaa accggtaaac
    14041 tgactttttt tcatggttct tctgccacat ttgatcaata cttccagcct ttggcagaat
    14101 tttcgagctt ttagctccct gcttcgccgg aaagctttga caatttatac caacaaccag
    14161 gaatttgcca acatggcatg aaagctaacg ccttgaggga taaaaaatgc cagcaaagca
    14221 tgctggcatt ttttttagta tcatgtaagg gttactgcct acttggtagt atcacttccc
    14281 atatttcctt cggttgggtt aaccgggaag gtgggaaggg cattgggctc ggcagtattt
    14341 ttcacctttg attcgataac agatttctgc tcttctgcgc ttttctttgg catagccaga
    14401 gtggcgaaaa gtgtaaaaag tatgagggca accgccaatg tccaagttga tttttcgaga
    14461 aaatcggctg ttttcctcac ccccatcacc tgatttgacc ccgaaaagtt agaagccaga
    14521 ccgcctccct taggattctg aatcagcact atcaggatga gcaggatgga aacaataatt
    14581 ataagcactg agaatagcgt gtacatcgta ggagttgtta atgttaactt attttgttga
    14641 ttccttaatc ttccgaattt cgtcggcaaa gtaactactt ttttccggat atttcaaact
    14701 taatttttcg aataccctaa ttgccttttc aaggtaaccc tggctgaggt agatttgggc
    14761 aagcgattcg ctggccagct cctccggttc ttccaccgag ctgatagaaa tatcctcatt
    14821 ctcaccggtg tccttattac ccggggttgt agaacgagaa atggtgggct ggttgtttat
    14881 aaatttagct ataaggtctt cgctctcatt caagcctctt ccatttgcct gggaatctac
    14941 ttcaccttcg ccctcagcaa gttccagaat ttcggtgtcg gtggtattac tacttgcctc
    15001 aacatgaatg gttattgctg gctgtttttc gtcatcgagt agtatgaatg agggtttcct
    15061 ttcatcggta ggagccttac ggggacgtcc tcttttaggt gtaggtttgg agattttttt
    15121 atcttcagca ttaccgccat ctattttttc agtctcttct agtggttttg aaggatatag
    15181 agtaaagtaa agatagcgtc gtgaggggag caaaacagca ttttgccgaa gttgagcctc
    15241 aaactcatcg tttcccagtt gcgaaagact tttgagaata agtactcggc acccttgaaa
    15301 gtaggggaac tcatcaatgg tttccctcag ctggttaagg gtttcgggtg taggcttcag
    15361 tcgtgcctcc attatgccgt atagggtttc attatccata gcgctaccag tttgctgctg
    15421 ctgcgttgaa aatatcatcc actagcttct cgactatcaa ccggaccagt tcggcttcga
    15481 cctgcacaaa gttctggttg ctgtcgaagt tttcgtactg cgaaaaggtt ttatcgaagc
    15541 tgagcttggg gtccttggcg ttaacaaatt tgaccctgac agaaattgtg aggcggtttt
    15601 gcgctgctac ctcgttgtcc tggattgaaa ttggtgaaac gttatactgg gtaatctgcc
    15661 cggagaaggc gaaatcgcca aaatcgtcaa ccattgacag gctggtttgg gtgataaagc
    15721 ggtttttgag ctcctcggtg aggtagctgc taagggtggg gttgactatt ccggcaaggt
    15781 tttgaaaaag ctgaacgctg aaggttttgg tctcgggaga aatggatgca ccggtaaagg
    15841 tgtagcttac cttgcacccc ggctgaatta agacaaacag gggaagaaga ataatagcta
    15901 ttcggatgat tcgcttcata tattagtcga gtttgtactc cttgatttta cggtaaagtg
    15961 tacgctcgga gattccaagt tcggaagcag cttgtcttcg gcgaccgtta tgcttatcca
    16021 gagcttttcg gataagctcc acctcctttt cctccaacga aagcgactcc tccattattt
    16081 cctcggtgtc ctgaatttcg ttgttgctct ttacaaacga catgggttca aaggtgggtt
    16141 gtgctggcga ggtaataatc gggttcaccg gtccaaagtt gttgtgcagc acggctgaat
    16201 cggcaaagga agattcagca ccggtaacgc cactctgcat gatgacgttg acaagctttt
    16261 taagctcgtt cacgtcgttg cgcatatcaa agagaatctt atagagaatc tccctttcgg
    16321 aggagaagct tggttcttcg gtagcaccct ttttggagta aagggtggga agtcggggta
    16381 cgttgttatc ggggatgtac ttctgaagga tatccgccgt aatcacccgg ctttgttcaa
    16441 taatggatat ttgctcggta acgtttttga gctggcgaac attgcccggc caggagtaat
    16501 cggtaagcag cttgcgggcg ttttcgtcaa gggtgatggg gggcatacgg tacttttcgg
    16561 agaagtccac ggcaaatttt ctaaagagca ggtaaatatc ctcctttcgt tcacgaagcg
    16621 ggggaatgta tatggggacg gtgttgagac ggtagaagag atcctcccgg aacttaccct
    16681 cctgaatggc ttcatagagg ttgacgttgg tggcggccac cacccttacg tttgttttta
    16741 tcaccttaga ggagccaacc cttatgaact caccagtttc aagaaccctt aacagcctta
    16801 cctgggttga gtggggaagt tctgccacct cgtcgagaaa aatggtaccc ccattagcca
    16861 cctcaaagta accctggcgg ctgtcggtag cccctgtaaa cgaacccttt tcgtgcccaa
    16921 acagctcgga gtcaatagta ccctcgggaa tagcaccaca gttaacggct atgtagttgc
    16981 catgcttgcg tgcgctaaac tggtgtatta tctgaggaaa aacttccttc cctgtgccgc
    17041 tctcaccggt aataagcacc gacaggtcgg taggagcaac ctggcgggct atgtcaatag
    17101 ccctgtttaa tccaggggca ttcccaatta ttccaaagcg ttgttttatg gtttgtagct
    17161 ccatgacatt tccctaacac ctgacaaaat tacatatttt tctcaatctt gtattaatgc
    17221 cttaggataa aaaatgccgt tgattttaac tttcgaacag ggaggttaca gcatgaacac
    17281 tttttagcca cacagccagc tgtctagacg cgctgatttt taaacatgta gcattttctt
    17341 ttacggaaat ttcaacatgg gcttgtattg caaccgctta ggtgcgttta gttctagaaa
    17401 atttgggctt tttccaaagg tagtaaacag ggattcccaa aataactatg ataaggcctg
    17461 gccatgtgta ggcaggttta tagataagca aaattaccat aatgaatgta caggcaagaa
    17521 tatatactgc cggtgtaact gggtagcccc acgttttata aggacgagct acgtcaggtt
    17581 tttttaccct taagatgaaa acccccacca cggaaagcac gtagaaaatc agcactgaga
    17641 atatcacata atccagcagg ttgctgtaag tgcccgaaag gcacagaata caagcccaag
    17701 cacactgaag cgccagactg acaccaggta cacccataga attaagtatg cctacctgtt
    17761 taaagaacag gccatccttg gccatggcgt agtaaacgcg ggccccggat agtatcatcc
    17821 cgttaacaca tccaaaggtt gaaatcatga tgagaatggc catgatgatg gctgcagatt
    17881 cgccaaatgt tccatacata gctgctgtgc ctaccctgtc gttcgaggcg tactgcattc
    17941 cctgtcccat caccgttgtg gcatcgggcg atccacgaac gggtaaaacc ttaatgtata
    18001 tgtagtttac cagcatgtag agcactatga caataacaac gccaagcacc atacttaacg
    18061 ggatggttcg tttggggtcc ttaatctcct ccgaggcaaa ggttacatta ttccatgcgt
    18121 cggcggcaaa caacgatcca accatggcta tacctaaagc aataataagc gaaatgcccg
    18181 aaaggggaac aggtttacca tcaaccagct gctgcccttg ccagaatatc tccctgttga
    18241 gccttacggc ttcatcattt ctggcaataa aaaaaccaac aatgaaaaat agaagcaatg
    18301 ctgcaatttt tgtgtagctg aatgtatcct gaacatattt tccaatgttt attcccctgg
    18361 tgtttaccca ggtaaggaag agtataatga aaatagacat taggtggatt gtagtgattt
    18421 tcaagctgcc aacctgcagc aaaacattgc tatcggaaaa ccacggaaat atcaccccaa
    18481 gaaacttgct aaacgcaact gaaactgcag caatggaacc ggtttgtatt acgagaaaga
    18541 gcgtccagcc aaataggaaa ccagctagag gattgtaggc ttctcttagg taaacatatt
    18601 gtcctccggc atggggcatc atcacggcca actcaccata gctgagtgct gctataacgg
    18661 taagaattcc ggttattgcc cacgcaacca gcaaccaccc gggcgagccc agatttctgg
    18721 ccatatcggc gctaaccaga aatatgccag agccaatcat gctacccacc actattgctg
    18781 tggaatcaaa taagtttaat cgccgttgaa aagtggttgc tggctttggc ttcattccaa
    18841 tctcaataag ctacttctta ggggttgcct gtaaacagat gcaagaaaat taaaattatt
    18901 ctgattagca ataccaaacc ccatgaaacg gtatgctcca ttcaaaaaaa atgcttcaac
    18961 caaaaccttt attggtgtct gcatacaacc ctgtaattag gagcaacaag cgactattat
    19021 ggaatggata gactgccagg tagaagtccg ttaagtatgc gaaagcacgc aagggctact
    19081 cttcgtgcag attatgattt tagctgaatt ggtgcagctt atcagcgctc gtgctgtaac
    19141 tcactgaaaa aggcaaatct tacttaagca gcatttcaaa atcggcctga aggggttggt
    19201 tattctcaag gtcgaagagc gttaacctgc gcatctggta gtaaaactgc cagtagtttc
    19261 ccagggtgct gatgtacttt tgcttggcct gatcgcgctc ggactgggct acgtttaaat
    19321 cgagcacatc aatcttgccg atgaggaatc gttgcttggt aacctcgtac ctgttttgtg
    19381 aaatgtagtc ggctttttcg gcaatcataa cctggtcgtc ctgacggttg aactgcatca
    19441 cctttaaaaa gatatcctgc tcaaaatcag tcatggcctg ctgtacggta gttttaatta
    19501 cctcgcgatt cgattgggcc attttcacct ttcccttgcc caatccccaa tcgagaatag
    19561 gaatggtaat acccacccta acattctgct gatcctgcgg gtccttgtag gcatcagaaa
    19621 cattgagtgc cctctgggtc aacccatagg tagcgtatag gctagcgtta aatccccttt
    19681 cggaacgggc ttgagccacg tcacggtccg cttcgatgag ctgccgttcg taggaaagaa
    19741 tttgcggatt gttctccctg gccagcatca tcaccttgtc gtaaataagt tcaaattggg
    19801 gaattgctgt gggtagtatt atttccagat cgtactggtc gtttagcccc aggaacgact
    19861 taagctgaat tttcttaagc tgaaggtcca cctcagcctc gttaacctga gttccggcat
    19921 taaggaagct gagctccatc tgtagcagct cgttttcggc aattgtaccg atgttgtagc
    19981 gcccttgggc aattttataa agggtgtcgg tgttggagta gttaagcctg gccgtttcga
    20041 ggttttgctg ggccatggca aggttaaaaa acaacctgct ggcctcaatg gaaatatcct
    20101 ccatggactg gataaagctt ttcttggcct cctcgtactt aataggctca attcgcttat
    20161 tccactttag gcggttcatg gcaaacaggg gctgggagaa accaatggaa acaggcacgc
    20221 taaggtagga agtggagctg ctgtcgccca gcaggtctgt tcgttccagg ttgcttttaa
    20281 cgaaaatctg gcctccggta aacccaatat tttgcgataa cgataggccg agtgaggtgg
    20341 tgttggcatt ctcctcaatg tagctatacg acccgtcagg attttggtag cgaattagac
    20401 ttctgctgaa ttcgggaatt gttccgttca tgctcagcgt tggcctgtat tctgccacaa
    20461 atgtacggta ctcccagtat gccgctctaa acctatgctt ggcaaggatg gcctggggcg
    20521 attgttcttt ggccagctgg agcacctccg ggtaggttag ccggttcatg ggctgctggg
    20581 caatacagct cattaccggc attgctgata gtatcccgaa tagtagggtt gacaacctgt
    20641 tcatctcaac aaattattag cgtttttact cgtagcgtaa cgactcaata gggtcttttt
    20701 cggcagcacg cttggcaggc gacaagccaa aaatgacgcc cacggcagcc gataccccaa
    20761 atgcaaccaa aattgaaagt gggctgacta tggtaagtat tccggcaaag tgcctgatta
    20821 gaaacgatag cacaaagccc agtatcactc caataattcc gcccgaaacg ctgatgagca
    20881 ctgcttcgga aaggaattgc gcaattatgt cagctttctt ggccccaatg gccaggcgtg
    20941 ttcctatctc ctttatacgc tccataaccg aggcaaacat gatgttcata attccaatcc
    21001 cgccaacaat gagcgagatg ctggcaatgg cccccagcac aatgttgaat atgtccttgg
    21061 tgcgctgttg ctgcttgagc agcagctctg gaacggttat ctcaaaatcc ttcacctcgg
    21121 aatggcgcct gagcatcatc cggctgagca cctcggaggt ggaggtaata tcctcggttt
    21181 ggttcacctg caggataatc cggtcgagct ggtggtagtt ggaggccgct tgtgattgcg
    21241 tgctacctcc ataggagaca aacccaccgc tgacgaatac gctagttccc gattcactaa
    21301 tcttgcgggt gtttaccagc gccctgttct ggtagcgaag cagtatgctt ttcaccggca
    21361 cgtagatatt atcgttgtac acgttaatac cgctgctttc aaatccggta accttaatgt
    21421 ccgatttagc cagcacgcct atcaccctta gccatacact gccaaacttg atgtactgcc
    21481 caatgggatt ctgggtgcta aaaaacttgg tctttatatt tgaacctata acacaaaccg
    21541 gaacaccatg ctcctcgtgg tagctgtcga agattacccc atcctccagg ggaaggttaa
    21601 agagggaaaa gtagtcgttg gatactccca taattttagc gggcatacga attccgtcgt
    21661 actggacaaa cgagttaagg ctgatttcgg ggctaatcct tttaaccgag gggattatct
    21721 gctgaatggc ctccacatcc tttagtgtca gcccgggcga aaatttctgt ttttgcttgg
    21781 tgccattctc ctcctccgag gtttgggtgt cgggtgcctc gaatatggag ttaacgatga
    21841 tgttgttaac gccaaccatc ttcatctgct ccagtatctc ctgctgggca ccattcccta
    21901 tggccaacat gctgataact gcagccacac caaagattat gcccagcgct gttagggccg
    21961 attttagctt attggccata attgcctcaa tggcaatgtt gatgtcgtgc aggtagcgct
    22021 tgtagagctt gtcaactcgc agcatactca tcaggattta ggagcggttg gtttagggtt
    22081 tgcttggttg ctaccagagt ttcccttccc tttaagctca ctgttgattt tcggagctga
    22141 gccatttttc ctcacctgtt gcccattcat ctgaaacatc attttctggg cctgagacct
    22201 gcttttcatt gcagaatcct gcacctcgcg aagcctcatt tcatcctgaa ttttttgctc
    22261 ctttttcttc tttataaccg aaatgagctc cttccccgca agcgaaaact tttcgggttt
    22321 ctcgggcatc gaaaggtaaa cctcatcgtt tgcctccagc cctttttcaa taatgatgaa
    22381 attctcattc atttcgccag gaaccaccac ctgcattttc cccttacggg tatagacgta
    22441 gctgatggag tcgttcgcat ggagcgcctc gagaggaatg taggttaccg agtcgtaaac
    22501 attggtaatt atctggttac cggttgtcat ggagggtcga agtatcgggt catactcatt
    22561 taccctgatg gttacctcaa aaaccttggc atcagtgttt gggagttgct cgccaatatt
    22621 ggctacttca ataacctcac cggtgaattt tttttcagga aaggcatcaa cggtaatacg
    22681 aacgggttgt ccggttttaa ttttactgat gtctatttcg ttaacgtagg tgctggagtt
    22741 cattaccgaa aggtcgggaa gggtggcaac aatcaaatcc cagggtgaaa cgcttgaccc
    22801 caccttgcgt ttttgcccat tccactcgcg gtagtagata agcatacccg gcttgggtgc
    22861 acgtatttca aacttatcaa gcacatcaag catatcctgc ctttctcgtt tttggcgttg
    22921 aaggttgatg gccacctcct tcattgaggc ttcggcctgc tcctttttca gggtatagtt
    22981 gtgaacctcc tgctcgtatg cacgctgagc cttatcaagg tttatctgag cctgccgttg
    23041 ggtagccggt ggttcatact tcgactgctc cagcaccagc tgtttctcct ccatgtcaaa
    23101 cttcaggttg ataaggttgt tccttagctc gcgcagctgc agggtggtgt cgagttgaac
    23161 tttcaggaaa gcctgaaggc ttttctccag ctcgtcctcg gtgcttttta gctggttgct
    23221 aagcgcagcc ctatcgagcg ttgccacgta atcgccagag tccacaaggg ttccttcggc
    23281 tatcaaatcc tgaattttaa cctccgagat tcggaatgtc cggcctcgca gctcggttgg
    23341 ggccatgatg ttctctgaat tttgagcttt cagctcgccg gttacgttaa ccaaaacctc
    23401 aaattttccc tgcataaccc gggcaaactg tggtgctgcc ttgtccttat cggacgatcc
    23461 aaagagaact accgcaagta taattgccag aacaacggcc aggccaatga ttaccttttt
    23521 tctcatattc accttctgtt tttgctaaga atttgagttg aaaatagcaa ataggtttaa
    23581 gctggtaaaa cattctggcc taaaaatata aaatatgcgc taccctactg gcctcttcaa
    23641 cttttaatat tgtcacatct cctaatgtcc tgaaaatttt aggtttattg cctaacaaaa
    23701 gggatgggga ggcttttcaa aatactgctc taatttttac tgcaaaacta tactacaaag
    23761 ttgtcgagga gtaaaactcg gaacccgcca tcgcactgct tggccatcag gtttgatggg
    23821 aaaatcaccc ttttttttta cactttcaac atcaacagcc gaacaaaata aaattaccaa
    23881 ttatcccata tctgatggtt tttaaaatca acatttccgg ccgattatca tcaattcaac
    23941 caaaagccac tcaacagttt taggttctat cggctaaagc agcctttgcg agctgctgcg
    24001 agctgctgat tattcggtaa acatgtatct tcaaaaaaat tacctttaca acaatttata
    24061 aaccacgcta atggttacaa aaatttccgc aatggttatg gtagcaacaa ttggaatggg
    24121 ctgtataaac gcccaacagc cagactatgc acaatgggtt aaccccttaa ttggaaccga
    24181 caatatgggg cacacctttc ccggggccac cgttccattc ggttccatcc agctaagccc
    24241 cgacaccgat accgttccct actcgcttga tggtaaaagc tacaatccag aggtttaccg
    24301 ctactgcgcc ggctaccagt attccgacaa gacaattgtg ggtttcagcc acacccattt
    24361 tagcggaacg ggacactccg acttaggcga tattctggta atgcctacgg tgggccagct
    24421 gcagcttaac cccggtacat ccgctaatcc cgaaaagggc taccgttcga gatatagcca
    24481 caacaacgag tatgccaagc cgggatacta ccgggtgatt ctcgacgact acaacatcaa
    24541 ggctgaactt actgctacca cacgcgttgg tgttcaccgc tacacctttc cccagtctga
    24601 ttcggcccac attatcatcg acatggtcca cggcatctac aactatccag aaaaaaatgt
    24661 ttggactttc gtcagggtcg aaagcgatac gctggttaca ggctacaggg taacttcggg
    24721 ctgggccaga acgcgcaccg tttactttgc catcgccttc tccaaaccct ttaacaccta
    24781 tggcctggtg aacgacgaaa acttggctta caagggtttc tggcgtaagt ttaatcaaaa
    24841 cgagaatttt cccgaaaatg caggccgacg ggtgaagtgc catttcgatt tcactaccgg
    24901 cagcaacgag gccattatgc ttaaagtggc catatcgccg gtaagcaccg caaatgccat
    24961 tgagaacctt aaaatggaag cccccggttg gagcttcgat aacatggctc aacatgccag
    25021 agacatttgg aaccatgagc tgggaagaat tgatgttgcc acactcaacc aaaacgacaa
    25081 ggtaaacttc tacacctcgc tttaccatgc ctacctggga cctacccttt acatggatca
    25141 ggatggaagc tacatggggc tcgaccaaaa cattcataaa gcaaccgggt ttgaaaacta
    25201 taccaccttt tcgctttggg atacccatag ggcgctacac ccccttttca acatcataca
    25261 acccaagcga aacgcagaca tgattaggtc aatgctggca cactacagcc aaagcgttca
    25321 tcacatgcta cccgtatggt cgcactacgc caacgaaaac tggtgtatga ttggatacca
    25381 tgcagtttcg gttattgctg acgctgcagt taaaggaaat acggcatttg acttgaacga
    25441 agcgcttaat gcagccgttg caactgccga aaatgactgg tatgatggcg ttggctacta
    25501 catgcagtac ggttacgttc ccgaggacct atcgcccaac tcggtctcca aaaccctgga
    25561 gtacgcctac gacgactggt gcatctccca gcttgcagca aaagcaaatc gacccgacct
    25621 tgcagccagg ttctaccagc gatcccgaaa ctacctcaat gtttttgaca aggaaatacg
    25681 ctttatgagg cctcgcctct ccaacggaaa tttcaaaaaa gagtttgatg ttctgagcac
    25741 ccatggacag gggtttatcg agggtaactc gcttaactac tcgctctatg tgccccatga
    25801 ccccgaaagc ctggcttcac tcctgggtgg aaaggaaaag ctggccagct accttgatac
    25861 ccttttcacc aagtacctac ccgatgagta ctttgctcaa accgaggata ttacacgcga
    25921 agggctgatt ggaacctatg ttcacggtaa cgaaccctcg caccatgtgg cctacctcta
    25981 caactggtct gcggaacctt ggaaaacaca ggaaaaagtc cgaatgattt gtcaaaccat
    26041 gtataaaccc acacccgatg ggttaagcgg taatgatgat tgtggccaga tgagcgcatg
    26101 gtacattttt agcgctctcg gattttatcc ggttgctccg gcatcggagt tttactccat
    26161 tggtagccct accgtggtaa acgctacaat taacctggaa aatggcaaaa ctttccgcat
    26221 ttcaacctta aatcaatcac ctgcaaatat ttacgtgcag aatgcaatcc ttaatggaaa
    26281 accattaacc gggttaaaat tgtcccattc tgacataatg agcggcggtg agctgcagct
    26341 ggtaatgggg aaaaaaccaa taaaaaggat gtaaaatata ggggctgcca ggctaaattt
    26401 cattctctaa aacctttata catcctgtta cgtgtgggta ttccaccagg tgtaaagaaa
    26461 ttttcacctc ttaaaccagg cgcgggcacg agtgctcttg atagctttgt agaaaatgga
    26521 gggctacccc acccggacca attcagccag gtgcaacgta aatttttcta cctttgaagt
    26581 gttgttaaac tgttatggcc gattcgcttg acaaaccaaa gggcttcatg ataaaaccaa
    26641 aagtgctaac gggttttatc atcattctgc tattctcggc ctcatccatt atagtaactt
    26701 accagggttt cagggagctg aaccttaccc ggcaggagat tggacaaccc ggccaaaaac
    26761 tagccgtcat caaccggctg ataagcgggg tgtacggagg tgaagccgac gtaagaatgt
    26821 tcattattac caacgacacg ggctacctcg aaagttttga ggaaagaaag aaaaaggtta
    26881 catcggtgct caactcgctg gatggcttaa tggctgaaac gccagggcag caacagaatg
    26941 ttaagcaaat aaaagcctta ctttcacgga aatgggcaat tgtaaatgag ctccttgcac
    27001 tcagaaaaac ctatactgac gataggtttt acgaggaggc gctaaaacag attgaaagag
    27061 ccggaaccga aagccgtata agggcatctg tagccaaaac aaccaccgta accaccaccc
    27121 tgcacgacac ggtggtaacc ctcgagaaca aggatgaaaa cctttttgga aaaataaagc
    27181 gatttttcgt tggtcccgag agggttgaca cggtttcgcg aagcgttaat atcgaaaccc
    27241 ggctcgatac tattgataag gggcagtacg tttccgattc gataatcaac aacctggtta
    27301 agctcattaa tggcatcaga gccgaccagc gtaagtacca attcgacctg ggcgtaaagg
    27361 aagccgagct gctggagcag gacagggagc tgatgaaaaa gatagagagc attgtttcat
    27421 cactcgaaag gcaggagctt tcttcgtcca tacgccaatc ggttcttgcg caggacctgg
    27481 taaaccgctc catcataaaa attctagcgc taggctcctt cacctttatc ctgctgcttg
    27541 tcctgctggc tgttatattc agggatattt cgcgaagcaa ccagtaccgt acccagctgc
    27601 tggaagctaa gcagtatgcc gaacgtctcc tgagagtaaa ggagcaattt ttggctaaca
    27661 tcagccacga aatacgaacg cctcttagtg cggttattgg tctaacgcac cagctcggga
    27721 aaaccgactt gaatgccagg cagcaggact acgtcagcac gctcagtagc tcagccgatc
    27781 acctgctgtc ggtaatcaac gacattttgg actactccaa gctcgagtca gggcaggtgc
    27841 gatttgaaac agcaatgttc gacccggtga atgtactgga aagcgttact cagaccttta
    27901 atccgaaagc tgaagaaaag cagctgctcg ttcacaccaa cattgaccct gccgttccgc
    27961 atgttttatg gggtgattcg tttcgcctga aacaggcgct tatgaacctg cttagcaatg
    28021 ccattaagtt caccgaaagt ggaagtatta tagtcagcct tggcgtggta aggctaactc
    28081 ccgagtatgt taagctgcag ttcacagtgg ccgacacggg agttgggatt ccccctgaac
    28141 agcagcaggc tatttttgag gagttcaccc aggccgacag cagtatttcc aggaaatatg
    28201 gaggaacagg acttgggctc acgctggtga aaaagattgt tgagcttcag gggggtgaaa
    28261 tttcgttgga aagcaaaatc aacgaaggaa caattgttaa gttctccatt ccctacgcct
    28321 taacaggggt tgaaacgaag gaggagactt tagaaagaag cagctttgcc ataccaaaca
    28381 atacggctat tctggtaatt gacgatgacg aggttaacct gctaattgta accgaaatgg
    28441 ctactactct cggattgcag gttgatacgc tgtctaagcc cgaagcactc tacgaaactc
    28501 ttagcagcaa gaagtatagc gccattctta ccgacatcca gatgccgggg cttaacggct
    28561 acgaggtggt gaagctggtt gaacagttca acgaaaatat tcctgttgtg gccatcaccg
    28621 ccaatagcat ggttgacagc ccggagcact tcaccagtca tgggtttgcc gggtacctga
    28681 taaaaccctt tgtggaaacc gacctgcaaa aggttctgtc gcccctgatt ggcattccaa
    28741 aaccggtgaa gcagctgcag gttagcacaa aaagcaggcg tagctctccg gcacagattg
    28801 atctgtcaga tttataccga tttaccggcg gtgataagaa ctccctcagg ttgatactaa
    28861 cctccttcct ggacaacacc taccgtaatg ccgacgagct gaaccggtat attcgtgaaa
    28921 aaaatttaaa gcaggcctcg gaagttgccc atcgaatgaa gtcggcattt agccagctca
    28981 aaatctatca catttcgtcc ctgctccaaa aaatagaaac gctcaagccc gacaagtaca
    29041 gggctgccaa catctacctc gactacctta acaagcaaat taaaccggtg gtaaaagaaa
    29101 ttcgtgagct gctggagaag ctgtagccgg ggtaaacccg aagttttccc aaacgtcgga
    29161 ttttaatcag gtcaaacaat ccttacaccc attaccacaa catcgtcaat ccgtggagta
    29221 tcgccctgcc acgaaataag cgtgtcgagc agaatttgcc tttgctcggc cattgggtag
    29281 gtgtgaattt tcagaagcag ctccctgaag tccttaatca tgaattttcg ccccgactca
    29341 ccgccaaact ggtcaacata gccatcggag aaggtgtaca tcacatcgcc cttctgaatt
    29401 tcgatgacgt tgttggtaaa cggcacttca ccacgaagat gaattccaat gggcattttg
    29461 tcgcccttta cctgggtaac ctctccattt cttatcagga taagcgggtt atttgccccc
    29521 gcaaactgca tggtcatgga ttgctcatca acaatgtaga tggtgatgtc catgccgtcc
    29581 ttgctaccgc caacatcgct ggtctggtgc agcgtgctta taaccagctg ccgcagccta
    29641 ttcaggatgt cggctgcctg tagctcctta acctcgctta atatttgggt aataaaggag
    29701 atacccagca tactcataaa tccacccggt acgccatgac ccgtgcagtc ggcaactata
    29761 catatttttt tgctttccac ctgggttacg tagtaaaaat cgccactgac aatatccctg
    29821 ggtaagtata gaatgaagtg ctcgggaaat atcctgctga ccagctcgga tggtgtaagc
    29881 agtgcgcttt gtatccggct ggcatactga atactgctgg ttatctcttc attctgaatt
    29941 gaaattttct ccttttgcct ttcaatttcc tccttttggg ccacaacctc agcagtgcgc
    30001 tcctcaacaa tcttttcaag tcgttccttt tcagcaatca gccttcgggt gttccacctt
    30061 accaaccccc aaaccagccc tataaaaaag ataaagtagc aaaaatatgc cagaagggtt
    30121 ctgtaccatg gtggatggat gacgaaggtg aaggtagccg gaatactttc ctcaccaaaa
    30181 atattgacag cttttacatg aaaggtatat ttcccttcac tcaggttggt ataggtaacc
    30241 tcggttctcc catcccatgc gctccaggta gtttcatccg aaccctccag gtagtggctg
    30301 tagagcagct cggcctcctc ctcaaaatag ggcgaagaaa agctaaaaac taagccattg
    30361 taccggtact ttagctgggg aatcttgctt tgaggttgct gcagaagggt aatctttcta
    30421 ccatcctggt ccacctcgga aaaagagccg ttgaaaagga gggaatcctt ggcataaacg
    30481 cttcgaacca aagcatggta tggagcgttg tagctccgct taaccctggt attgaagctg
    30541 aacagctcct tccctatccc aatccatagc accccgccat catcaacata cagagcatcg
    30601 gctcctttgt tgggaagcct tttaaaaggc ttgtcattgg taacccattc gccggatgaa
    30661 tccatcgcca tcgtgcacac ccagtacttg ccagtagaat cgttgtaaca ggcggcaaca
    30721 atttcattcc ccaccttcaa caaacgtgaa aagccgtttt ccaaagaatt tttacccaac
    30781 aatccacatt tcctgaaggt gctatcgctg tagctatagt aaaatatgcc ctttggagta
    30841 agaacgtaaa ggctgtcgcc atcggggtaa atacgtagca gcttaaaacc ggagaaatcg
    30901 ctgctggagt taaagttgta aactttttcg tggggatcaa tcatgtaaac gcccttaagg
    30961 tttgtcccca gccaaagatt tccccttgcg tcctcgccaa ttgaccatac ttcggagcga
    31021 atctcatcaa gggctatgtt ctgggttttc acccattccc ccttgctcca ttcaatgtag
    31081 gtaagcctcg aaataccccc aacgtatagc atattaggat gaagtacgga ctggtaaagg
    31141 gtaagacact ggtgctgcac gtttggaaaa gaacggtaaa ggggtttgct tatcgaaata
    31201 gccctgttgc ccttaatttc aaaaacccca tcaacatacg aaccggcgag tagcattggc
    31261 gagcctcccg gaggagtgaa ctttaacaat gaccacacaa cgccattgat accctcaacc
    31321 ggcttaaata ccgacattcc gttagcagcc gccgtttgat agtaaacccc gttgatggtt
    31381 gcaacgtaaa gcgtgccatt aaactgaata atatccagaa ttagctcgtt gattccgctt
    31441 tcggtcgaaa accgtcggat gggcgaatgg agctctattc gggaaattcc tccgtcgaac
    31501 agtgtggccc acaccggcga ttcgccattc tggtaaagat aggtaatttg agcggcctga
    31561 agccctgttt gtgggtttac tatggtgctg ggcgaggagg aatcggcgtt tagctcaaag
    31621 aagctcagcc agtcggactg caccgttcca accccaatat tcccgttatg cagcttgatg
    31681 gcatgatagg gaattaccga ctggcttaac atcgactcga taaaattatt ttgcttctcc
    31741 atctttttgg agctgccggt cttttggttg taaacaaaaa aaccactatc ggtaaccagc
    31801 agcagctccg atgggcttaa agacactatg gagtagatat taatgcgttc aaaactacta
    31861 cctccgggag ctgttgataa cgtccaatcg ctattcagca ttcgtaaacc cttcaggtag
    31921 ctgccaatgt aaactttatc gttcactaca aaggtgagaa aattggcata ctctggctga
    31981 tatcctaggt aaaccggcgt tagcttgcct tgcttatcat aaacaaaaat gtacgagcag
    32041 gaacagaaaa gaacgttgcc cttgaagtag tatgttttgt aaatatgcga aagcgattgt
    32101 tttatggtgt cgttaatgga acccgaaagg gagacaaatc gttgcgaacc atcggctgtg
    32161 ggttctaaaa atccaaaatc gcccgatgtt cccacgtaga taaccccatc atcaccctta
    32221 atcatggagt tgaccggttt gctatccgga acctgaattt ttctccaggt ttttccatca
    32281 tactctagaa ttcccttatc gtaggtgcca aagtacatga cacccctgtc gtcctgggta
    32341 atacaccagg tctgttcagt tgcatcgtaa tcctcaggcg taaaattggt taccaacggg
    32401 ttccccagcc tgttataggg ttgccccgtt gctgtgtaaa cggttatgcc aagtaatgcg
    32461 aaaaggaagt accttctcat agggttaatt ttctggtttt aaaagtatga aaaagaaaat
    32521 caccatgcaa atttgaaaaa actaatcctc ctaaaaacaa aatactatta agttcgaaag
    32581 gcagcaaaca actgtcagct caattctacc gatggttgcc agataatgca ggttgcataa
    32641 tttcaacatt taccatccta ctatgaacct aacttgatgt tgaatttctc aaacaataca
    32701 gccagctcta ccatactatt agatttcatc atggcagatt tataggttcc tgagggttaa
    32761 cattactaat aaaattgagg catcgtaacc ccgttacccc ccaccaggct taaacaaaaa
    32821 acttccagca ataaacatgc ctactaatca aaactttaaa attattgtta cttttgtgca
    32881 aggaaatgca acaacatgag aatttcacgt ccagcagttt tgattatagt ttttgcaagc
    32941 ttgatgcagg tatcgtgcaa ccgtaaggct cagctgctct acctgaacaa cttagacacc
    33001 ggacttttaa cttcaacgca gcctgcaccc taccagctca ggccggggga tgtgctaagc
    33061 gttcaggtta tcacccagga tcccgaaatc agccgcctat tcaacattgt ttcgagcggt
    33121 acctctacct cctacactgc ttactcaaac gatgcctcgc tttacataaa cggattctcg
    33181 gtaaacgact ccggcatggt acgcctgccc gttgttggaa cgatgcccgt agcagggctg
    33241 accgttcgcc aagctcagga ttcgatacag cttaaatatg aaaggtacct caaggatgct
    33301 ctcgtggtgg tgaagctgct cagctttaag gttacggtgc tgggcgaagt aagacgaccc
    33361 ggaacctaca ccaactatag ggacaacctc aacatatttg aggcaattgg acttgccggc
    33421 gatttctccg attacggtga acgaaccaac gtgcttgtgc tccgccaaac acccgagggg
    33481 gtaaaaacat tccggctcaa cctgcaggat aaaaatctgc ttaccgccga ggggttctac
    33541 ctgctgccaa acgatacggt tattgtggag ccacgaaacg ccaaggtgat ggccctgaat
    33601 acccctaaca tttccatatt cctttccatc atcaccaccg ccttgctgct gcttaactat
    33661 gtaaaataaa gaggaaatta caacggttca tgttggtggt aaatcgcaac taaagggtaa
    33721 aaaaacattg aagggagacg gtatggaaga caacatgaat cttaaatcca acccacaaca
    33781 gcaggacctc cccgaggagg agcagctgga tgttaaaaag ttgctgttcc gcgtgctggc
    33841 caactggtac tggtatgcca tcacactctt tatctccctt tttatagcat atctcatcaa
    33901 ccggtactcc gaacagtcat tcagcgtctc atcaagtgta attgttcgcg acgacgacca
    33961 gactaagtca tacgttggcg ccgagcagct catacagagc ttgcggctgg tgaagaatac
    34021 caagagcgtt cagaatgaaa tcgggatact gcagtcatac tccctggccc ggcaaaccgt
    34081 ggaggagttg gaggatttta acgttaccta cgtggccgtg ggccgtaggg gaataaagga
    34141 acagaagctt tacaccggtt ccccctttaa ggtagtgata gactccggtg caaccaacat
    34201 gcaaggccat cctgttaggg ttaacatcct gaacagcagg gagtaccgtt tggagattga
    34261 tgagggaatg ggcatttcac gtaccatgag gtttggcgag ccattcacca acgagtcgtt
    34321 caactttaca gttttcctgc gcgatccatt gaattatgac cccaaggttg cagggcgaaa
    34381 gtactacttt accattaacg atcccaacac cgtagcccta tcgtacaagt cgaaagttac
    34441 ggttaccctt aacgacaaaa agggttcaat tcttacgcta aacagcacag gctacgtggc
    34501 acaacaggag gccgactacc tcaacaagct catggaggtt tacatccgac gagggctgga
    34561 ggagaagaac cagattgccg agaatacaat taggttcatt gaccgccagc ttgaggacat
    34621 ggccgactcc cttcgcagtg cggagttaca tctgcaggat tttcgcacct ccaaccgggt
    34681 gctggacata acaggcgagg gcaacctgct gctcgaacaa ataaaaacca tacaaaacga
    34741 gcagagcagc atggagctca aagcacgcta ctacgcctac cttagcgact acctcgaaca
    34801 gcgtaaggat ttcaacaccc tggttgcccc atcggttatg ggggtggacg acccacagct
    34861 caatgccctg ctttcggaaa tatcaaaggc ctacatagac cgcgaggagc tcaagactac
    34921 cgttagggag ggtagccccg gccttgaaca gctgaacatc cgcattgaaa accttcgaag
    34981 ctccctggcc gaaaaggtga agagccttat tgagtcaaat gccattgccc agcaggagct
    35041 gaactcacgg ctaagcaggg ttgacgctga gattaaacgc ctccctgtca acgaaaggat
    35101 gctgataggt tttgagcgcg acttttcgct gatagacaag atgtacacct acctgcagga
    35161 gaagcgcgcc gaggctgcta tagcgcgcgc ctcaaacatc cccgacaaca aggtgctgga
    35221 ctatgcaatt gcccaaaacg cttcgctcat caaacccaaa aggtcaacca actaccttat
    35281 agccctgctg gtggggctgg ccattccgtt catcatcatg ctactctacg actttttcaa
    35341 cgataaaatt gttgacctga aggagatatc caaacgaacc cgggtacccg ttataggaac
    35401 gctggggcac aaccgctacc aaacggagct gcccgtgctg gaaaagccca agtcaaccct
    35461 cacggagtcg ttccgcgggc tacgaaccaa cctgcagtac ctgatgcgtg agcccgaatc
    35521 cagggtagtt tcggtatcct ccaccgtggt gggcgagggt aaaactttca tagccgtcaa
    35581 cctggcagct gccattgccg ttaccggcaa aagggtgctg ctgcttggac tcgaccttcg
    35641 aaaacccagg ctgcacaggc taatgggcta ctccggcgac atcggggtaa gcacctacct
    35701 cattggccag aacagcgcag gggatatagt gttcccgtcg cagcacgaga acctgtactt
    35761 tgcccccagc gggcccatac ctcccaaccc cgccgagctg ctcggtacag cccgcatggc
    35821 ggagctggtt gcctgggcaa ggcagaactt cgacttcatc gtggtggaca gtcctccagt
    35881 tgccattgtt tccgatatat tcattattgc ccgcttcacc gatgccaacc tgttcaccat
    35941 ccgctacggc tacagctcaa aagaggtgct caacctgatt aacaatatct acaggcacca
    36001 cgaagttcgc aacctcgcca tggtaatcaa cgactttgaa ccccgaaggg gctacagcta
    36061 cggatacggt tacgcctaca gctacggata cagctacgga tacggctaca ggtatgggta
    36121 tggcccctac acgaacagcg atggctacta ctccgatgag gacgagccca agccaacatg
    36181 gaaggagcga atcaaaaggc tgttttagtc ccttttctgc tccattttgt tgagctatta
    36241 gttttaaatg caccgtccta cgggcgatgt aactgacagg gcgaagctgt aagaaagcac
    36301 tagcagttat gatatccaca gacttacaca tattaacaca gatgattaag tggtatttgc
    36361 gaaaatttgt gtgcatttgt gggaaaaacg aaagcatgct acgtttccgt tttgcattaa
    36421 aaatagtgaa ttttagaaat gtattacatt gatttgagaa ataaatttat taatcttggt
    36481 tgctttaata tcaatcagat atatgcattt gaccctgatt ttaatagaaa taatctaaca
    36541 tattggcaga aaaaaggttg gcttatttat cttcgcaatg gttattatgc ttttcccgaa
    36601 tttaaacagg aaataaattc tcaatatttt ttagcaaata aaatatacag accatcttat
    36661 gttagcttgc aatctgcatt atcttattat gaaatcatcc ctgagtctgt tgtgcaaatc
    36721 acaaatgtta ctacacttaa gactaaatcc tttgaaaatg attttggaca attttactat
    36781 aaaagtataa aatcagattt ttatt
//
LOCUS       DAOSHM010000024        34687 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_979_length_34687_cov_20.733137, whole genome shotgun sequence.
ACCESSION   DAOSHM010000024 DAOSHM010000000
VERSION     DAOSHM010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 34687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34687
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_979_length_34687_cov_20.733137"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            62..790
                     /locus_tag="PK839_05470"
     CDS             62..790
                     /locus_tag="PK839_05470"
                     /inference="COORDINATES: protein motif:HMM:NF015527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase family 2 domain-containing
                     protein"
                     /protein_id="HOG72220.1"
                     /translation="MIVSHKHKFIFIKPAKTASSSVELMLSKICGPNDVLTPLSSFND
                     RFDGDYYEYQPRNHKGFENHMSANDIKKRVGDKVWYEYLKVTIVRNPWDMVVSRYHWE
                     KARLWPGIRRNIVKILRNPFAIVRYKPLRHRIKMIFVLASFSNFIKYFDKSWTNSRFY
                     FDKEGKPICDIYLSYENLNEGISALESLLGVQLGSPPHLKNKPRHRKNHYSQYYNQKQ
                     KEQIEKLFKHELEIFDYTFERVAQ"
     gene            1319..1939
                     /locus_tag="PK839_05475"
     CDS             1319..1939
                     /locus_tag="PK839_05475"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HOG72221.1"
                     /translation="MFSGIVEEPATVVALRREMENLHITLKCSFAGSLKIDQSVSHNG
                     VCLTVVSREGDTYTVTAIRETLEKSNLGLLKVGDKVNVERSMRIDSLLDGHLVQGHVD
                     QTAVCTGVEEAEGSWYYTFEYDPSKGNITVEKGSIAVNGVSLTVVNSRSSSFQVAIIP
                     YTYEHTNFHQIQKGTVVNLEFDIVGKYITKMLRQYIESGQIANLSR"
     gene            2005..3066
                     /locus_tag="PK839_05480"
     CDS             2005..3066
                     /locus_tag="PK839_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG72222.1"
                     /translation="MNADRLIKSPQAVALIISVISALIAGVVSAAIFGVATEKQVLSC
                     VAIAMASMAGTFIASTILVSLFAARKIKPLYKILYQVDVKNSDIANEFEDNNLLENIN
                     ASLVSWTTSKTEEIDRLKEMERYRKEFLGNVSHELKTPIFNIQGYVLTLLDGGLADSH
                     INRKYLERTEKSINRMIGIVEDLEIISKLEAGELKLNMERFNIVGMVEDIFEALEERA
                     RKKSISLEFDKVYERPVWVYADKKRIYQVLHNLIINSINYGIQHGHTVVAFSDSGKRV
                     TTWVTDDGIGISQKDIPRIFERFYRVDKSRSREQGGTGLGLAIVKHIIEGHKQSIKVE
                     SSLNKGTTFSFTLDKPTKQ"
     gene            complement(3128..4141)
                     /locus_tag="PK839_05485"
     CDS             complement(3128..4141)
                     /locus_tag="PK839_05485"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOG72223.1"
                     /translation="MILVTGGTGLVGAHLLYELTSRGESVRALKRERSNTEFVDWVFK
                     LYSHESSKLLQGIEWVDGDLLDYQSLLDATKGVQTVYHTGGIVSFNPLQSSSVIETNI
                     TGTGNLVDACMDNGVKEICHVSSIAALGQANESGMVDEDCRWIESKGQNAYAVSKLYG
                     ENQLWRGFAGGLRVIVVNPTVILGPGRWDSGSGQLFKRVYKGMPFYTNGVTGFVDVRD
                     VARAMVVLMEETGINGERFILNSENVSYRDIFTMMAASLGKRKPSIRVPAGVVDAVYP
                     LVQLFGQLSGRGNLVSKANLKTAFRVTLYSSQKIREKIGFEFIPIEETIRFVGKAFRN
                     AEP"
     gene            4382..6076
                     /locus_tag="PK839_05490"
     CDS             4382..6076
                     /locus_tag="PK839_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019129894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HOG72224.1"
                     /translation="MASENLNEELASERTKNFLEEIIEEDIKSGKHGGRVLTRFPPEP
                     NGYLHIGHAKSICLNFGLAQRYGGKTNLRFDDTNPEKEDVEYVNSIKEDIRWLGFQWA
                     GEYYASDYFEQLYEWAEVLIKKGLAYVDDQTQEEIRINRGTVNQPGKPSPWRNRSVDE
                     NLDLFRRMRAGEFGDGERVLRAKIDMAHPNMLMRDPIMYRIIHAEHHRTGSKWCIYPM
                     YDYAHGQSDSIEKITHSICTLEFDVHRVLYEWFIEKLEIFPSRQYEFARLNLSYTVMS
                     KRKLLELVREKIVMGWDDPRMPTICGLRRRGYTPESIRYFADKVGVAKRDNVIDLSLL
                     EFCLREDLNKRAERRMAVLSPLRVLITNYPEGQVEELDAINNPEDDAAGTRSVPFSRE
                     IYIEQDDFMENPPKKFFRLAPGQEVRLRYAYFIRCNEVIKDEQGNIIQLNCTYDPASR
                     GGNSPDGRKVQGTIHWVSAQHAIKADVRLFDRLFSKPDPNDVPEGVDWKSTINSDSLR
                     VVEAYIEPSLKNVKPLDKFQFERLGYFSVDYDSTSQKLVFNRTVTLKDTWAKIAGK"
     gene            6182..6254
                     /locus_tag="PK839_05495"
     tRNA            6182..6254
                     /locus_tag="PK839_05495"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6215..6217,aa:Gln,seq:ctg)
     gene            complement(6263..7735)
                     /gene="cysS"
                     /locus_tag="PK839_05500"
     CDS             complement(6263..7735)
                     /gene="cysS"
                     /locus_tag="PK839_05500"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009129741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOG72225.1"
                     /translation="MDSKLHLYNTLSRTKEEFKPINPPHVGLYVCGPTVYGAPHLGHA
                     RPAITFDLLFRYLTHLGYRVRYVRNITDVGHLENDADEGEDKIAKKARLEQLEPMEVV
                     QRYTDLYHDAMRKLNVLPPSIEPRASGHIIEQIELIKRILDNGYAYVRNGSVYFDVAR
                     YNERYPYGKLSGRRIDDLMANTRQLDGQEEKQNPFDFALWKSADPAHIMRWPSPWGEG
                     FPGWHMECSAMSIKYLGQPFDIHGGGMDLLFPHHECEVAQSVAASGHEAVRYWIHNNL
                     ITINGQKMGKSLGNFITLEELFTGSHKLLQEAYSPMTIRFFMLQAHYRSTLDFSNEAL
                     QAASKGFARLMAAIRTLDRIPPSIKSTVDVSDLQNRIYEAMNDDLNSPVAIAALFEWV
                     KIINLLAEGKETISAADLETLGSMFRTFTFDILGLVDEQQSASKHTELAGKLIELLLA
                     MRLEAKRQKDFATSDRIRSELEQLGVVVRDRKDGFDWEIS"
     gene            complement(8335..8766)
                     /locus_tag="PK839_05505"
     CDS             complement(8335..8766)
                     /locus_tag="PK839_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72226.1"
                     /translation="MATCWVLITVSAVSAQTANADAEKALKAEKVAFITSKLELTPKE
                     AKKFWPVYDAYWDKKNEVLRERRALTGELTKIIEQSPDDKTLLAYADRYVENRQRETE
                     LLVELNEELKEILPPRKILLLYKANHEFKNYLLQKVQETGK"
     gene            complement(8787..9251)
                     /locus_tag="PK839_05510"
     CDS             complement(8787..9251)
                     /locus_tag="PK839_05510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72227.1"
                     /translation="MEAKKNGLEVIEGKAGKELPFSVPDGYFDSLPRRIQERCTQPQH
                     QQPKQSWIFALRSQLALAAGFALLVVAAYFGYYTTTRSIPRDSYTLQKSDYLNIVSRS
                     ISEFDDIDLYKAIENKRKTDSIDSAARTLQQRYFIKKGNATTIIGEKYEEKP"
     gene            complement(9303..9851)
                     /locus_tag="PK839_05515"
     CDS             complement(9303..9851)
                     /locus_tag="PK839_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOG72228.1"
                     /translation="MECYSDKELLEMFRNGETRNFAFNLIVNKYQERLYWHIRKIVVG
                     HDDADDVIQNTFIKVWGGLETFREESQLFTWLYRIATNEALTFLKKKKVKSFLSMDDV
                     ESQLAKTLETDAYIDGNELQLKLQKAILKLPEKQRLVFNMKYFEEMKYEEMSEILGTS
                     VGALKASFHHAVKKIEKFMGEN"
     gene            complement(9859..10815)
                     /locus_tag="PK839_05520"
     CDS             complement(9859..10815)
                     /locus_tag="PK839_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HOG72229.1"
                     /translation="MKYTSTGNKDTRSGFGAGLYELGKVNPNVVALCADLIGSLKMED
                     FSREFPDRFFQTGIAEANMIGLSAGLTIGGKIPFAGTFAVFATGRVYDQIRQSVAYSN
                     KNVKICASHAGITLGEDGATHQMLEDIGLMKMLPNMTVINTCDYNQTKAATIAIANHV
                     GPVYLRFGRPAVPNFTPADQQFTIGKAVKLTEGTQVSVFATGHLVWKALEAADMLEQQ
                     GISAEVVNIHTIKPLDEEAIIRSIQKTGCAVTAEEHMMNGGLGDSIAQVVARIHPVPL
                     EMVAINDTFGESGTPEELMVKYGLDAPSIVKAAKRAIARKNN"
     gene            complement(10944..11342)
                     /locus_tag="PK839_05525"
     CDS             complement(10944..11342)
                     /locus_tag="PK839_05525"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF003559.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HOG72230.1"
                     /translation="MKNIALIAHDAKKPELARFLKDHEEWLPGVNLLATGRTAEFLEE
                     QGIKCRHLSPGQSGGYNEITGMIQRGEVDIVIFLRDPKVVQQHHEDIRLLLEACNIKN
                     IPLATNKATAELVILGLIHMEAIERMKRNK"
     gene            complement(11364..12209)
                     /locus_tag="PK839_05530"
     CDS             complement(11364..12209)
                     /locus_tag="PK839_05530"
                     /inference="COORDINATES: protein motif:HMM:NF012670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOG72231.1"
                     /translation="MTDFEFLNKMAAQVRRDVIRMVHRPSSGHPGGSLGCADFFTALY
                     FDVLRADPKHFTMDGTGEDLFFLSNGHLSAAWYSVLARRGYFDVKELSTFRLLNSRLQ
                     GHPTPAEHLPGIRIASGSLGQGLSVGCGAALAKKLNGDSHLVYTLHGDGELQEGQIWE
                     AALFAAAKGIDNLIATVDYNGKQIDGPVDTVVGLGDLKAKWLAFGWDVLETNGNDMPA
                     LVKTLHEAVEHTGKGKPVIILMRTQMGMGVDFMVGNHKWHGKAPNDEEFAKAMAQLPE
                     TIGDF"
     gene            complement(12322..13137)
                     /locus_tag="PK839_05535"
     CDS             complement(12322..13137)
                     /locus_tag="PK839_05535"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010264780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HOG72232.1"
                     /translation="MVNLQEIIDAAWENREMLGTPEVNDAIKSVVEMLDKGEVRVAEP
                     ADDGWKVNEWVKKAVLLYFSICSFQATGAGPLEFYDKIPLKKNYQSLGVRVVPHAVAR
                     YGSYIARGVVMMPSYVNIGAYVDEGTMVDTWATVGSCAQVGKGVHLSGGVGIGGVLEP
                     VQAAPVIIEDGCFIGSRCIVVEGVRIGRESVLGANVAITRSTKIIDVSGSQPVEYKGM
                     VPPRSVVIPGSYTKQFPAGSYQVACALIIGKRKESTDLKTSLNNALRDFGVSV"
     gene            13356..15755
                     /locus_tag="PK839_05540"
     CDS             13356..15755
                     /locus_tag="PK839_05540"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOG72233.1"
                     /translation="MRKLMLLSVLLVVSLGLQSQELVRYQVPPAEMVAILEAPPTPGF
                     LLSPDGKHALLSYPQVLPDLAELAKPELKLAGFRVNPENFGRYDSRIFDRLELLNISN
                     KMVTSLSGIPDSGHITSFSWSPDGKNVAISVYFNSSVELWIVEVESGVARPLSASLNE
                     SVISPGFAWHPDGKSLYVLKHREGMKKPGREKLPLGPSVQQATGQKSQVRTFQDLLKS
                     PADEELFEYYATSQLYRVNLLGEASEVGNPGIISQVEPSPDGSYIYVEQVVRPFSYIV
                     PAQYFPRLCHIWNADGHLVKRLAEQPLADDIPSGFSAARKGKREMQWRSDVPSTIVWV
                     EAQDDGDPHRDVKIRDVVYSLDAPFRGEPVKLVELETRFSSISWGWSNLAVAYGRWWS
                     SRRQTASLFNPSAPTDRPRVLWDRSWEDAYTDPGQLVMVNNANGFSVLQTNSSRTKLY
                     LSGKGSSPEGDMPFLDEINLKNLGVKRLWQCHAPLYEYFIGFYDMDNSRIVTSQESVN
                     RQPNFFIRDIKRQKLVQLTNFPDPYPGLHGVHKELVTYRRPDGVTLTGTLYLPAGYEK
                     GTRTLPTLMWAYPQEFLSADFAGQVTGSPYRFIRPSVLSPILWVVRGYAVFDNMSMPI
                     VARNGGEPNDTFIEQLVENAKAAVDTLVGMGVANPQKVAIGGHSYGAFMTANLLAHSD
                     LFAAGIARSGAYNRTLTPFGFQNEERTYWQAPEVYNRMSPFMHADKINEPLLLIHGEN
                     DNNSGTFPLQSERLFAAIKGHGGTARLVMLPYESHGYRARQSVFHTIWEMDCWLEKYL
                     K"
     gene            15971..16774
                     /locus_tag="PK839_05545"
     CDS             15971..16774
                     /locus_tag="PK839_05545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72234.1"
                     /translation="MVGFRKIWVLIFFVVFCKVSFSQEKLLPFDTLSIVHFRVINANT
                     REPVERAYILNLTYNWGSISDLLGYSYLPIHFGDKLKISAIGYYTEELICVGQFNADS
                     AFYEIKLKPRVYELNEVKITKFSTYERFLRGVINLNIPKTRDDKQLEVIEKYLFGVVK
                     KMDLKNTPTMASGIAFGKDWYAKQNEKLAEQLEREKTRRVIDEKFNPGLVQKLTGLSD
                     KELYEFIGYLDFSDEFLLQATDYEIHQKIMEKYNVFMAGKSNELKNQEK"
     gene            16771..17754
                     /locus_tag="PK839_05550"
     CDS             16771..17754
                     /locus_tag="PK839_05550"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HOG72235.1"
                     /translation="MSEENSSCCQPEASDVVSAYELIKDKVKHTPVHSSASVNALLGA
                     TLFFKCENFQKVGAFKYRGATHAVLKLNDEEARKGVATHSSGNHAAALALAAANRGIP
                     AYIVMPENAPAIKKKAVAGYGGIITYCRSTQQDREETLRVVQQQTGATLIHPYNNINV
                     ICGQGTAALELLDEMPDLDVVIAPVGGGGLLSGTATFIKGQYPHVKVFGAEPINADDA
                     FRSLRDGKIYPSLSPSTIADGLLTSLCPLTYSILRSKCDGIFTVSEKGIVNAMRLIWE
                     RMKLVVEPSSAVPVGVIAEHPEAFRGKRVGIIVSGGNADLDHLPWLDQSNG"
     gene            complement(17744..18781)
                     /locus_tag="PK839_05555"
     CDS             complement(17744..18781)
                     /locus_tag="PK839_05555"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOG72236.1"
                     /translation="MFALGLLFCYASVYINPARFWIPAIVGLFYPFFLLANIAFLTYW
                     IIVWKKELLISLLAILLGINHLSAFVQLRPGNKHESAVPSIRLISYNVNLFRLYPWSK
                     LPPSYDGVIRYIGSNNFDIACLQEFYTVSTKFSEADAKRKLGMRSKIGYIIQNSRSAY
                     GLAIFSRFPIVNSGEIKFENTLNSSIYADVKINGDTVRVYNLHLQSLRLKEHNFNFLL
                     NNELRQGNDRYDEIMDLASRLRIAFIKRSQQVELVNRHLKNCPYPVILCGDFNDTPVS
                     YTYRELTGKLHDSFKEAGAGMLNTYAGILPSYRIDYILHSPEFKTLKFSSPRLNLSDH
                     YPVCANFTLTH"
     gene            complement(18831..19718)
                     /locus_tag="PK839_05560"
     CDS             complement(18831..19718)
                     /locus_tag="PK839_05560"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOG72237.1"
                     /translation="MSISKEIKDSFRFGSSLTQLIYINLAIFLIYSLVRVIMFIGGTQ
                     PGSSYAHWLAVPAGLHQLLSRPWTLLTYMFFHENFLHILFNLLWLYWFGRLFLQHLSQ
                     RQLVGVYLLGGITGAIFFIAAYNILPVFRAISSEAMALGASASVLAVVIAVSTLKPNN
                     SIYILFLGEVKLKYLALATIIIDIISIPVSNPGGHIAHLGGAVFGFSFIKLLNRGTDL
                     TKFLNIFSRVKKPKAHRSKNLKVSYRRPETDYEYNRRKANEQKSIDRILDKIAKSGYD
                     SLSKEEKETLFRMKNDSVN"
     gene            complement(19905..20726)
                     /locus_tag="PK839_05565"
     CDS             complement(19905..20726)
                     /locus_tag="PK839_05565"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOG72238.1"
                     /translation="MSLSRSGFLSSIPPVVKNLILINTVIWLVTWMLQRYGVDLNAML
                     GLYTPGSPHFKPYQVVTHMFMHGGFTHLFFNMFALWMFGRILEQVWGGKRFLLYYMVT
                     GLGAAAVHSLVNYLQFSSIQADSIAMLNTLSPDTFAAFVTKHFPEFYNSIYSDFLDKW
                     FQNPSNPIMIDQAQQLSQQLLSLQLNIPTVGASGAVYGVLLAFGMLFPNTVLMILFPP
                     IPIKAKWMVILYGGLELFLGLTQAGSNIAHFAHLGGMIFGFFLIKYWQSRKNVFY"
     gene            complement(20797..22641)
                     /gene="mutL"
                     /locus_tag="PK839_05570"
     CDS             complement(20797..22641)
                     /gene="mutL"
                     /locus_tag="PK839_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HOG72239.1"
                     /translation="MADIIHLLPDSIANQIAAGEVVQRPASVVKELLENSIDAGCKAI
                     QLIVKDGGKTSIQVVDDGIGMSETDARLCFERHATSKIKDAADLYSIRTMGFRGEALA
                     SIAAVAEVELRTRRLSDEVGSMVQVGGSEFVAQQPVSCPVGSTLTVKNLFYNVPARRK
                     FLKSNSVELKHIISEFQHVALAFPEISMSLSHNGSEIYKLAPSNLKQRIVNLFGKSIS
                     SQLVDCVAETSIVAISGYIGKPESARKSPGEQFFFVNGRFMKSPFLHKAVVNAYNRLL
                     PANSIPSYFIYFRVDPGSIDVNIHPTKTEVKFEDDGAVWQIVHAAVREALGKFAVAPS
                     IDFSSDPGFSIPHFPKDSTVELPEIELNPNFNPFEDEGARFAQRTREHNFTPQQPVKG
                     WEQLYQDVNPNFNFEPKQTAVQTFASKGFDDSVAENTGRFFQIKGKYIITAVKSGMLV
                     VDQKRAHERILYEQFYKNLNLEKNVAQRELYPQTIELNPQDFETIITNQENFLRLGLD
                     ISNLNHNTISINSLPGNTRITDPALFLENLLAIINENEALPFDSAKEKIALSMAKAAA
                     VGYGKRLSESEMQEVVDQLFACREPNYTADGKKTIALIEMSEFEKWLK"
     gene            complement(22656..22880)
                     /locus_tag="PK839_05575"
     CDS             complement(22656..22880)
                     /locus_tag="PK839_05575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72240.1"
                     /translation="MVNNFFFGAQEHRDTIFWVKTHKGIVRGSVDRANSAFTPERMGA
                     MWIRHPDSYLPTRNILEVLTILLLVAHLIF"
     gene            23089..23736
                     /locus_tag="PK839_05580"
     CDS             23089..23736
                     /locus_tag="PK839_05580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72241.1"
                     /translation="MKPAGAVELIILTAASMLNGCTKNVEPFPTSATLSPSLSVPLGE
                     GGVSLTKTLQTLGIPVVNLSEDVPQWATYGFVYVADTIPLNLTEVYNRGDSITYLMVR
                     TNIWNQFPLGGRAQVFFLDANNQVIDQLYEDMVSVGPAEHGSHGEVVNTAFESNETSF
                     NTSRISLLSAAQKVVIYAGLEITDEGVSESNITYFDQYQLRVQVALQVDFIMNLN"
     gene            23746..25179
                     /locus_tag="PK839_05585"
     CDS             23746..25179
                     /locus_tag="PK839_05585"
                     /inference="COORDINATES: protein motif:HMM:NF040164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5723 family protein"
                     /protein_id="HOG72242.1"
                     /translation="MALRRIVNLLIFFSAYSTIVCAQQDNTLFLLHNIPQSNFTNPAV
                     QIQCPVYVGVPLLSSVHFNVNSTGFSYGDVANGSGVANLNALVAGMHRWDFVSEEVHF
                     TPVSFGFMYDRHQYISFAWTERVETKVFLPKKLLSLSVDGNTQYVGDELKTGNPGANA
                     VYYREFSVGYSKKVNSGLILGVHGKLLFGLAGAFTRRRPVNVDVDAITHNIDAQWNPK
                     VDVAYPLGVATDASGNVSNVTVGGFSPLNFFLNFGNQGIAADLGFIWQHDDVTWSGSL
                     LDLGFIWWHKQTTRFENSGHFVYRGATPADLDNPDDYINQLGDSLNNQIQVEYSRKGF
                     ITLLNPKIYLGASYPVSERFNVGAHVRAEWYPGRPVMGITLSGMFFTKKGGSAALSYS
                     VMNGSFINVGAGLGWGGQTFQFYVVSDNLMAALYPEKARNANLRFGFNLFFGCIEKKK
                     KVEVPHGNGCGCYWSWDDKTRRKLGRN"
     gene            25443..25979
                     /locus_tag="PK839_05590"
     CDS             25443..25979
                     /locus_tag="PK839_05590"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOG72243.1"
                     /translation="MKKICRIFAIAVWMAPLGLMAQPPEVGQVAPDILLSSPTGDSIR
                     LSSLRGKLVLVDFWASWCSPCRRENPNLVQTYNKFKDACFENGNGFTIYSVSLDQKQD
                     AWLKAIADDGLVWPYQVSDLKGWRSEAAKQYGVRAIPMNYLINANGVVVATNLRGDEL
                     DSTLRKLRKWRIFSNECI"
     gene            26011..29127
                     /locus_tag="PK839_05595"
     CDS             26011..29127
                     /locus_tag="PK839_05595"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HOG72244.1"
                     /translation="MKVGHILWKTAKIILITLGSLILLVVILLAAAKLSENKIVGLVL
                     NKVSVELKAPFSVREVNLLPLRNFPNLTVELQDFSVGKSTDSLQSGQSSNPFDTLFSL
                     HKLYVAVKAKPLLSSKVVVDQVEIDGATLNYHVDSTGASNFDFLMTTTDTTQVPEDTS
                     QVETVLNILLHDLTLSNIVLNYADDSLRAKACVAIPEIKLNGKVLGSYYAGAVEGSIS
                     VSDLALEGTHADRMGKTSLDFKVGYDNGDISVDKLNLNADGFGLSANGKAKLSDSIFV
                     DMGVGLTNMDFGRLTAYLPDTLLSSYGLTALGGIFDLKAHIKGFVYDTLLLPSVEASL
                     DFRNGFIKTTSYPTVETLAFNGKVKLPDPNNLKTASADFSTFRVKTHASNLNLAFKIL
                     NIEKPIYNVKGDMFITLDEFGSFIPDSLVKSLSGNIMARIETRGQLPDSLGMSSADYF
                     MQNTTLNVKLRNITAVLDSSTSVRGVNLNFDFLPGKQVAVENFTADLSIPGYHVGLDN
                     FLLRARLIGDIANTSKFGVDVDSLLIKVGNSSLGGRFKVMNLDKPTYSAKSFVNVNFN
                     ELRPFIPDTLVETLTGRMAATVQSHGTLNLDSLSSQIMPIAFEQSRFTLNLDSLNFAM
                     PNDTLMRVDNLSLKLAMSDDTIRINGFHGQAMGIEFAIDSTRIWNVYKAMLKGEKDKK
                     LVADTRIQLSDIDFAMFAPFMATDSTAAEPTPQPAEPQEKVAGSEAPATESNFTIPSY
                     IIRGTVAVNSFKYGNMLIDKISTKFRVDDSLYVIDDLKLNAFGGDLTLSALYDTRNDT
                     ATVIEAKCNLNRMNIRQLLADNNDFDQTFFTHENIEGLATGELFARVIMRDTNILYDK
                     INLLGNFKLENGGIYNFEPLMQLSKFTNLNELERVVFRTLETSVFVYNNEIYFPKTDI
                     VSSAMDISVYGMQSFADDYEYHLIVHLGDVLVGKSNKLLKQQGMESDVFEGQDKANRS
                     GLYLVALNRGKDSKYGFDTQHLQRLMKTTIRVQERGLNLIFNPKVVNFSTEIDRRERK
                     KKPNEAKG"
     gene            29111..29785
                     /locus_tag="PK839_05600"
     CDS             29111..29785
                     /locus_tag="PK839_05600"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOG72245.1"
                     /translation="MKLKVKLDRHHLIAYAISLVLVVLIGYKLWRYFTPDSKEAVLQE
                     SPEPRPLDIVFGSDTASLPVYMYASYSCTFCRQFFTDVFPMLKKEFLDNGRVKLVMRL
                     LENTNHPDALNSAKTAVCINRYGYFDKLHELLLTDSRVIYTPEFRDMVNDFTARDELV
                     AECILGGQADEYILQIRNEFRKFGLSGTPTFIINRNVYRGYRSYQEFRKIILHHMETE
                     DRKGIN"
     gene            29856..30227
                     /locus_tag="PK839_05605"
     CDS             29856..30227
                     /locus_tag="PK839_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72246.1"
                     /translation="MKNKIYLLPLSLLAVFTVVTWSCQKDDEEDVCQKFDPPQCEIAN
                     ACCPTDGGNCYYEYGDQKFYCDKSKATADDPDGCNAAEDQVIDVICSTAKGADIQLAK
                     VQLRELTRKLMNEARMLSVCH"
     gene            complement(30531..32450)
                     /gene="dxs"
                     /locus_tag="PK839_05610"
     CDS             complement(30531..32450)
                     /gene="dxs"
                     /locus_tag="PK839_05610"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HOG72247.1"
                     /translation="MGSDYRILPHVNSPNDLKGMKPEQLVELSEELRRFIVEVVSTNP
                     GHLGASLGVVELTVALHHVFNTPDDKLIWDVGHQAYAHKILTGRRDVFNTNRRYKGIS
                     GFPKRSESPYDAFGVGHASTSISAALGMAVASELKHESHQVVAIIGDGALTGGLAFEG
                     LNNGGSLKSNLLVILNDNHISIDPGVGALNEYLLDITTSKTYNRIKKDVWDLLGRFDS
                     LGPKARSAIQKLDNAIKTALLRQSNLFESLGFRYFGPVDGHDVAYLIRILNDLKDIPG
                     PKLLHCITVKGKGYTPAEQNQTIFHAPGKFNKDTGERISLLTSKPAPPRYQDVFGETL
                     VELAQSNDRIVGITPAMATGCSMNIMMEKMPERTFDVGIAEQHAVTFAAGMAADGLIP
                     FCNIYSSFMQRAFDQVIHDVALQNLHVVFCLDRAGLVGDDGPTHHGAFDLAYLRLIPN
                     ITIAAPMNEEELRNMMFTAQLDGQGTFSIRYPRGNGVMVDWRKSFTEIPIGKARILRE
                     GSDIAILTIGHVGNLAATAAGKLEAEGISVFHADMRFVKPLDADLLRSVAKRFTTVVT
                     VEDGCLPGGFGSGVTEWLNDNGYNVRVVRLGIPDEFIEQGPPQQLYAECGFDTENIYQ
                     TAKKYVQKKVKMRVG"
     gene            32878..34272
                     /locus_tag="PK839_05615"
     CDS             32878..34272
                     /locus_tag="PK839_05615"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOG72248.1"
                     /translation="MRAELLKKRALTRMVRLASNLSDEKLIRMVELSQRHLMRGVTDE
                     RVRFTAEKIKKLFEEKHPATELAKSTFRRVSKESRKRIVENFFINAGVTGRQRQEELK
                     RELGFGMPWFFVISPTAKCNLKCKGCYAGEYTKSEDLSFELVDRIFTEAKELGMYFIT
                     ISGGEPYMWPPLLDIFRKHSDMFFQTYTNGTLLTKDMAQKLAKLGNVAPAISVEGFEK
                     ETDARRGNGVFQRVMDAMDNLREAGVVFGYSVTPTRLNSELLVSDEFVDLMIQKGCVF
                     GWYFQYVPIGRKPDVSLMATPEQRNYIRQRTKEIRNTKPIFIGDFWNDGPIVGGCLAG
                     ARHRGYFHINCKGDVEPCVFLQFSVDNIKDKKLLDVIQSPFFRAIQDAQPYCENKNLL
                     TPCALIDNPRVLRDVVKQHNARPSYNSSYEVISNPDVCNFLDAYSRDYKKITDPIWEN
                     EVSLKYQSWKERFS"
BASE COUNT         9064 a         7929 c         8388 g         9306 t
ORIGIN      
        1 atacgaatgt ttttagtatt ttagtatagt ttttaacgtg tgatgatgcc gctggaggtt
       61 aatgatagtt tcgcataagc ataagtttat ttttataaag ccggccaaaa cagccagctc
      121 gagcgtagag ctgatgctaa gtaaaatatg cgggcccaat gatgttttaa ctcctttatc
      181 ctcttttaat gatcggtttg acggtgatta ctacgaatac caaccccgaa accataaagg
      241 atttgaaaac cacatgtcgg caaatgatat taagaaaaga gttggggata aggtttggta
      301 cgaatacctt aaggttacca tagttcgaaa cccttgggat atggtggttt ccagatatca
      361 ctgggaaaag gctcgattat ggcccggtat aagaaggaat atcgttaaaa tattaaggaa
      421 cccttttgca atagtgcgat ataaaccgtt acggcataga ataaaaatga tttttgtgct
      481 tgcctcgttc agtaatttta tcaaatactt tgacaaatcg tggaccaact caaggtttta
      541 ctttgataaa gaaggaaagc cgatttgtga tatttaccta agttacgaaa accttaacga
      601 agggataagc gctttagaaa gcctacttgg tgttcagctg ggaagcccgc cacacttaaa
      661 aaacaaacca agacatcgta aaaatcatta tagtcaatac tataaccaaa aacaaaagga
      721 acaaattgaa aagcttttta agcacgaatt ggagattttt gattatacct ttgagcgtgt
      781 agcccaatag aataaaatgc tagagaaaaa atttctattc gtagcaaatc aagattcatt
      841 aatttaggtt ttaataattc ttactctctg taaactgccc tgctaccgca caagtctccc
      901 cgtgtggtgg agagcagagc gtaagtggta gtagagaata ggaaagggag cggtagccgc
      961 tattaaaaag ctaatccggc atgattatgc tttgtaaatt taatagttgg gtgtgttgag
     1021 tgctctggtt gaggggtacg gacgagattg ccacacgtcg ggagacgtga ggcagctaaa
     1081 gaaccattac cttgacaggt agtggggtgc attataccct actcatacca agagggttag
     1141 cctttttttt aaagctttgc cttgcagagg tcatccttgg caaggtgtgg tcgagctgct
     1201 ttttgtcccg cactttcaag gcattttttg tatttttgca gtgtatatca gctagctctt
     1261 atttttaaaa cctagctggt aagggtttcg tagttgaact acctaaatca aattaatcat
     1321 gttttcagga attgttgaag aaccggccac ggtggtcgcc ctacgcaggg aaatggaaaa
     1381 cctgcacatc accctgaaat gctcctttgc ggggagtttg aaaattgacc agagcgtttc
     1441 gcacaacggg gtttgcctta cggtggtaag cagggagggc gacacctata ccgttacggc
     1501 cataagggaa acccttgaga aatcgaacct ggggttacta aaggtgggcg ataaggtgaa
     1561 cgtggagcgt agcatgagga tagacagcct gcttgatggg catctggttc aggggcatgt
     1621 tgaccaaacc gctgtgtgca cgggtgtgga ggaagccgag ggcagctggt actacacctt
     1681 tgagtacgac ccttcaaagg ggaatataac agttgaaaag ggctccattg ccgttaacgg
     1741 ggttagcctc accgtggtga actcgcgcag cagctcgttt caggtggcca ttattcccta
     1801 cacttacgag cacaccaact ttcaccagat acaaaagggg actgtggtga atctggagtt
     1861 cgacatcgtg ggtaagtaca taaccaagat gcttcgccag tacatcgaat cgggtcaaat
     1921 tgccaactta tccaggtagg ccatgaaggg agctgtcagg ttttacaaca gagctgtggt
     1981 ttgggtaacg aataacccat tcaaatgaat gctgatcgtc tgattaaatc tccccaggca
     2041 gtagccctca tcatttcggt tatttcggcc ttaatagccg gtgtggtatc ggctgccatt
     2101 ttcggggtag ccaccgaaaa gcaagtatta tcctgtgtgg ccatcgccat ggcatcgatg
     2161 gccggcacat ttatagcttc cacaattctg gtaagccttt ttgccgcccg aaaaatcaaa
     2221 cccctatata aaattctata ccaggtggat gtgaagaatt ccgatattgc caacgaattt
     2281 gaggataaca acctgctcga gaatatcaac gctagcctgg ttagctggac aacaagcaaa
     2341 accgaggaga ttgaccggtt aaaggagatg gagcggtacc gaaaggagtt tttgggaaac
     2401 gtgtcgcatg agctgaaaac gccaattttc aacattcagg ggtacgtgct gacgctgctc
     2461 gacggtgggc tcgccgattc ccatatcaac aggaaatacc tggagcggac cgaaaaaagc
     2521 ataaaccgga tgatcggcat tgtggaggat ttggaaatta tctcgaagct tgaggctggt
     2581 gagctaaagc tgaacatgga acggttcaac attgtcggca tggtagagga tatctttgag
     2641 gcgcttgagg agcgggccag gaagaaatca ataagccttg agttcgacaa ggtttacgaa
     2701 aggccggtgt gggtttacgc cgataaaaag cgaatttacc aggtgctgca taacctcatc
     2761 atcaactcaa taaactacgg aattcagcat ggccacactg ttgttgcgtt ttccgattcg
     2821 ggcaaaaggg taacaacctg ggtaaccgac gatggaatag gcattagcca gaaggacatt
     2881 ccgagaattt ttgagcgttt ctacagggtt gataaaagcc ggtcgcgcga acagggaggc
     2941 acagggcttg gacttgccat tgtgaagcat atcatcgagg gtcataagca gagcatcaag
     3001 gtggagagta gccttaacaa gggtacaaca tttagcttca cccttgacaa gccaaccaag
     3061 cagtgatgtg gaaacaaagt atgctgcaac ccaattgccc taaagccagt gtggaataat
     3121 tgttgcgcta tggctccgcg ttcctaaatg cctttccaac aaacctgatg gtttcttcaa
     3181 tgggtataaa ctcgaaccca attttttccc ttattttctg cgaggagtac agggttaccc
     3241 tgaaagccgt ctttaagttg gccttggaaa cgaggttccc tcttcctgag agctgtccaa
     3301 atagctggac aagtgggtaa accgcatcca ctacaccggc tggcaccctg atgctgggct
     3361 tgcgtttgcc cagcgaggca gccatcatcg tgaagatatc cctgtagctg acgttctcgg
     3421 agttaaggat aaacctttca ccgttgatgc cggtttcttc catgagcacc accattgccc
     3481 ttgcaacatc acgcacatct acaaagcccg taacgccgtt ggtgtagaaa ggcatgccct
     3541 tatatacccg cttaaagagt tggccgcttc cactatccca gcgtcccggc ccaagaatta
     3601 ccgttgggtt aacaacaata actcgcaatc ctccggcaaa gcctcgccaa agctgattct
     3661 ccccgtacag cttgctgaca gcgtaggcat tttgcccctt actctctatc catcggcaat
     3721 cctcatcaac cattcccgat tcgttggcct ggcccaatgc ggctatcgag cttacatggc
     3781 aaatctcttt taccccattg tccatgcagg cgtcaaccaa attgcccgtt ccggtaatgt
     3841 tggtttcaat cacgctcgag ctctgcaggg ggttaaacga aacaattcca ccagtatggt
     3901 aaaccgtctg aacacccttt gttgcatcaa gtagcgattg gtagtcgagc aaatcgccat
     3961 caacccactc gattccctgt agcagcttgc tggactcatg gctgtaaagc ttgaataccc
     4021 aatcaacaaa ttcagtgttg gagcgctcac gcttcaacgc ccgcacgctt tcgccgcgtg
     4081 aagttagctc gtatagaaga tgcgccccta ccagtccggt tcccccggtt accaaaatca
     4141 tactatttcc atctgttggt ttgctattaa gaacaagtat tctgcaccca ggttcaaaca
     4201 tagcaaaatt tcagctaaat tggtaaaaat caggaatgtt gaagatttac gggtgatttg
     4261 aagctgccat tatatgggcg gttacgtcta aacttaaggg aaggagtgta aaatttatta
     4321 ggtgctaata aactttagcg gcaaatttct acttttgctt ctgtgaatca acccaaatat
     4381 gatggcaagc gagaacctaa atgaggagtt agcttccgaa aggactaaaa attttctgga
     4441 ggagataatt gaggaggata ttaagtcggg aaagcatggc ggaagggtct taacccggtt
     4501 tccaccagag ccaaacggct acctgcatat tggccatgcc aaatcaatat gcctgaactt
     4561 tgggcttgcc caaaggtatg gcggcaaaac caacctgcgg tttgacgaca ccaaccccga
     4621 gaaggaggat gtagagtatg taaactccat caaggaggac atcaggtggc tgggattcca
     4681 gtgggcgggt gagtactatg cttccgacta ctttgaacag ctctacgagt gggcagaggt
     4741 tctcattaaa aaggggctag cctatgttga cgatcaaacc caggaggaaa taaggataaa
     4801 ccgcggcaca gttaaccagc ccggtaaacc tagcccctgg cgtaaccgat cggttgacga
     4861 aaatcttgac ctgttccgtc ggatgcgggc cggggagttt ggcgatggcg aaagggtgct
     4921 ccgtgcaaaa attgacatgg cgcaccccaa catgctcatg cgcgacccaa tcatgtacag
     4981 gattattcat gccgagcacc atcgaacagg tagcaagtgg tgcatttatc caatgtacga
     5041 ctacgcccac gggcaatccg actccatcga gaaaattacc cactccatct gcacacttga
     5101 gtttgacgtt caccgcgtgc tgtacgagtg gttcattgaa aagcttgaaa ttttcccctc
     5161 gaggcagtac gagtttgccc ggctcaacct ctcctacacg gtgatgagca agcgtaagct
     5221 gctggagctg gttagggaga agattgttat ggggtgggac gatccaagaa tgcccaccat
     5281 ttgtgggcta cgacgcaggg gctacactcc ggagtccatc cgctactttg ccgacaaggt
     5341 gggggtggcc aagcgcgata acgtgataga cctttcactt ctggagtttt gccttaggga
     5401 ggatttgaac aaacgtgccg aacgacggat ggcggtgctt agcccgctgc gggtgctgat
     5461 taccaactat cccgaggggc aggtggaaga gcttgatgct attaacaacc ccgaggatga
     5521 tgcggccggt actcggagtg tgccattcag tagggagatc tacattgagc aggatgactt
     5581 tatggagaat ccgccgaaaa agttcttccg gttggcgcca gggcaggagg tacggcttcg
     5641 atatgcctac tttatcaggt gtaacgaggt gattaaggac gaacagggta acattattca
     5701 gctcaactgc acctacgacc cggcctcccg aggtggaaat tcacccgatg gtaggaaggt
     5761 tcagggaacc atccactggg tatcggctca acatgccatt aaagctgatg tacgcttgtt
     5821 cgacaggctt ttttccaagc ccgaccccaa cgatgttccc gagggggttg actggaaatc
     5881 aaccattaat tccgactcgc tcagggtggt tgaagcctac attgaaccct cgttgaagaa
     5941 tgttaaacct ctcgataaat tccagtttga aaggcttggc tactttagcg tggattacga
     6001 ctctacatcc cagaagctgg ttttcaaccg caccgttacc ctgaaggata catgggctaa
     6061 aattgcgggg aagtagcaaa ggcgaaaagc ctattgcggc ttggtaggtc atggggaata
     6121 aaaattttcc cggtagaatt accagtaaaa ggctaattag ctatatttgc accgtaaaaa
     6181 ttgtcctgtg gtgtaattgg caacacatct gactctggat cagaagagtc caggttcgac
     6241 ccctggcagg acaacatttt ttctagctaa tctcccagtc gaacccgtct ttacggtcac
     6301 gcacaacaac tcccagctgt tccagctcgc tgcgaattct atcggatgtt gcaaagtcct
     6361 tctgcctttt agcctcaagc ctcatggcta agagtagttc tattaacttg cctgccagct
     6421 cagtatgttt ggaggccgac tgttgttcgt ccaccaggcc cagtatgtcg aaggtgaagg
     6481 ttcggaacat cgagccaagc gtttccaaat cggctgcaga gatagtctcc ttcccttctg
     6541 ccagcaggtt gatgattttt acccattcaa atagcgcagc aatggccaca gggctattca
     6601 aatcgtcgtt catggcctca taaatgcggt tttgcaggtc ggacacatca accgttgatt
     6661 tgatggaagg gggaatcctg tccagtgttc taatggcagc catcaaacgg gcaaaccctt
     6721 tggaggcagc ctgcagggct tcgtttgaaa agtcgagggt ggagcggtag tgagcctgaa
     6781 gcatgaagaa ccttatggtc atggggctat acgcctcctg caaaagctta tgcgatccgg
     6841 tgaatagctc ctcgagggtg atgaagttgc ctagcgactt gcccatcttc tggccgttaa
     6901 tggtaatcag gttgttgtgt atccagtatc gaactgcctc gtggccacta gcagccacgc
     6961 tttgggcaac ttcgcactcg tggtggggga atagcaggtc cattccaccg ccatgaatgt
     7021 cgaatggttg tcccaggtac ttgatgctca tagcggagca ttccatgtgc catccgggaa
     7081 atccctcccc ccagggcgat ggccatcgca tgatgtgcgc cggatcggcg cttttccaaa
     7141 gtgcaaaatc aaatgggttc tgcttctcct cctgcccatc gagctgtcgg gtgttggcca
     7201 tcaggtcatc aatcctccgt cccgatagct ttccgtaggg gtaccgttcg ttgtaacggg
     7261 ccacatcaaa gtaaaccgaa ccattgcgaa cgtaggcgta accgttgtcg agtatccttt
     7321 taatcagctc aatttgctca ataatatgcc ctgaggccct gggttcaatg cttggtggca
     7381 gcacgttaag cttgcgcatg gcgtcgtggt aaaggtcggt gtagcgctga accacctcca
     7441 tgggttccag ctgttccagc cgcgcctttt ttgcaatttt gtcctcaccc tcgtcggcat
     7501 cattttccag atgtccaacg tcggtaatgt tacgcacgta gcgcacccta taccccaagt
     7561 gggtaaggta gcggaaaagc aaatcgaatg taatggccgg tcgggcgtgc cctaaatggg
     7621 gcgcaccgta aacagttggc ccgcaaacgt ataatccaac atggggtgga tttatcggtt
     7681 taaattcctc cttggttcta cttagagtgt tgtataggtg cagcttgcta tccataagtg
     7741 tttgctgttt gatgaggctg aaaatcttgt tgatgtatag cggctatggg tttactccgc
     7801 agtaatattt tcccggcaac atcgcaaagc ctgagccggg tttgtagcat attttccttg
     7861 atggggtgct gcctgtttgg ttgcttcaat tttcgctagc atggctaatt caggaggttt
     7921 tagtgtcatg aaaaccatgt taccaggtaa aaattagtta gccgggtaac aatatgcgca
     7981 aaggtaaaac ttttaggcaa atgccgaatg gggatacgat gacccggtaa ttgttccccc
     8041 agccaccgaa ggtggtttta tttggagtaa caagaatttt tttactggga agtatcattt
     8101 tcgtggacaa caatgaatgg acgccgttaa ccatcgtcgg tagctttacc tacccatgga
     8161 ttagaaagac gagctgattt gcacgttttt ctgctaaggg cagccttttg aggaaatcga
     8221 agcagtaatc taagcaacag accaacaagg cgcttgtacc aataactcct gttgaattgc
     8281 tggataaagt tttactatgc aaaaaggtgt ggttgcagca aatataacca ccggttactt
     8341 tcctgtttcc tgaacctttt gtagcaggta gtttttaaat tcgtggtttg ccttgtagag
     8401 taaaagaatt ttacgcggtg gaagtatttc ctttagctcc tcatttagct ccaccagcaa
     8461 ctcggtttct ctttggcggt tttccacata cctgtcggcg taagctaaga gggttttgtc
     8521 gtcgggcgac tgctcaataa ttttagtgag ttcaccggta agggctcggc gctcccttaa
     8581 cacctcgttt tttttatccc agtaggcatc gtaaaccggc cagaattttt ttgcctcctt
     8641 tggggttagc tcaagcttgc tggtgataaa agctaccttc tctgccttca gagctttttc
     8701 ggcatcagca ttggctgttt gggcgcttac cgccgatacg gtaatcagca cccaacaggt
     8761 agccatgaaa attttaaggt ggccactcat ggtttttcct cgtatttctc tccaataatg
     8821 gttgtagcgt tgcctttttt tatgaagtat cgttgctgaa gtgttctggc tgcgctatca
     8881 atgctatcgg ttttgcgttt gttttcaatt gccttgtaca ggtcaatatc gtcaaattcg
     8941 cttatagaac ggctgacgat atttaggtag tccgatttct gaagggtgta gctatccctg
     9001 ggaattgagc gagtagtggt gtagtatcca aaatacgctg caacaacaag cagggcaaag
     9061 ccagcggcta atgccagctg ggatcggagg gcaaaaatcc acgattgttt aggttgttgg
     9121 tgctggggtt gggtgcaccg ctcttgaatt ctacggggta agctgtcgaa atagccatcc
     9181 ggaacagaga atgggagctc tttccccgcc ttgccctcaa ttacttctaa cccgtttttc
     9241 tttgcttcca tgatgattct aattcaactg tttctattac gatgatattt gtcagggttg
     9301 gtttaattct cccccatgaa tttttcaatc tttttcactg catgatggaa tgaagcttta
     9361 agcgccccaa ccgaggttcc aagaatttcg gacatttcct cgtacttcat ctcttcaaag
     9421 tacttcatgt tgaaaaccag ccgttgtttc tcgggtagtt taagtatggc cttttgtagt
     9481 tttagctgta gctcgttacc atctatgtag gcgtcggttt cgagggtttt ggcaagttgc
     9541 gattctacgt cgtccataga taggaatgac tttacctttt ttttctttag aaaggtaagc
     9601 gcttcgtttg tggcaatccg gtaaagccag gtgaagagct gcgattcctc cctgaaggtt
     9661 tcgaggccac cccaaacctt gataaaggtg ttctggataa cgtcgtcggc atcgtcgtgg
     9721 ccaacaacaa tctttcggat atgccagtag agcctttcct ggtacttatt cacaataagg
     9781 ttaaaggcga agttccgggt ttccccgttg cggaacattt ccagcaactc cttatcggaa
     9841 tagcactcca tttcaccatt agttattttt tcgggcgata gccctctttg ctgctttaac
     9901 aatactgggt gcgtcaaggc cgtacttcac cattagctcc tcgggggttc cgctttcgcc
     9961 aaacgtgtca ttaatggcaa ccatttcgag gggtacggga tgaatacggg caaccacctg
    10021 ggcaatgctg tcgcccaagc caccattcat catgtgctcc tcggcggtca cggcacaacc
    10081 ggttttctga attgatctga taatcgcctc ctcatccagg ggtttaatgg tgtggatgtt
    10141 aaccacttcg gccgaaattc cttgctgttc cagcatgtcg gctgcctcaa gggctttcca
    10201 taccagatgt ccagtggcga aaacagacac ctgagttcct tcggttaact tcaccgcttt
    10261 tccaatggta aattgctgat cggcaggggt gaagttaggt actgccggac gtccaaaccg
    10321 aaggtaaacc ggtccaacgt ggtttgctat ggctattgta gctgctttgg tttggttgta
    10381 gtcgcaggta ttgataacag tcatattggg caacattttc ataagcccaa tatcctcgag
    10441 catttgatgg gtagccccat cttcacccaa ggtgattcct gcatgtgaag cgcaaatctt
    10501 cacattttta ttcgagtagg cgacgctttg cctaatctgg tcgtaaaccc ttccggtggc
    10561 aaaaactgca aaggtcccag caaagggaat tttaccacca atggtaagac ctgcagaaag
    10621 tccaatcatg ttggcttcgg ctatgccagt ttgaaaaaat ctgtcgggaa attctctgga
    10681 aaagtcttcc atcttgagcg agcctatcag atcggcgcaa agggctacga cattagggtt
    10741 aactttaccc agttcatata gacctgctcc aaagcccgaa cgtgtatcct tgtttcctgt
    10801 tgaagtgtac ttcatccgtt gtaaatgtat ttttttaaaa caaaaaatct gttgaaaatt
    10861 gctgttggtt taaggtagtt ctgttaggag ttcaggaaag ccaacagcag attaccaaat
    10921 tggcaaacga gaatggtcgg tggttatttg tttcgcttca tacgttcaat ggcctccatg
    10981 tggataagcc caagaataac cagctctgcg gttgccttat ttgttgccaa agggatattt
    11041 ttgatgttgc aggcctctag cagtaagcga atatcctcat ggtgctgctg caccaccttg
    11101 ggatcgcgca gaaagataac tatatcaact tccccgcgtt gaatcatgcc ggttatctcg
    11161 ttgtaaccac ccgattggcc aggactaagg tggcggcatt ttatcccctg ctcctcaaga
    11221 aattcagcag ttctccctgt ggccaacagg ttaactcccg gcagccactc ttcatggtct
    11281 ttgagaaacc tggccaactc gggtttcttg gcatcatggg caattagcgc aatgttcttc
    11341 attgtataaa ttatttaaat agtttaaaag tcgcctattg tttcgggcag ttgtgccatg
    11401 gcctttgcaa actcctcatc gtttggggct tttccatgcc acttatgatt acccaccata
    11461 aaatcgacac ccatgcccat ctgggttcgc atgagaatta tcaccggttt acccttaccg
    11521 gtgtgctcca cggcttcgtg tagggtttta actagtgcgg gcatgtcatt tccattggtt
    11581 tccagcacat cccaaccaaa ggccaaccat ttggccttaa gatcacccag accaacaaca
    11641 gtgtcaacag ggccatcaat ttgctttccg ttgtagtcaa ccgtggcaat aaggttgtct
    11701 ataccctttg ctgcagcaaa gagggcggcc tcccagattt gcccttcctg cagctccccg
    11761 tcgccatgca gggtgtaaac aagatgacta tcgccattca atttttttgc cagcgcagca
    11821 ccacaaccca ccgataaccc ctgaccaagc gatccggatg ctatccggat gccgggaaga
    11881 tgttctgccg gggtaggatg cccctgaagc cttgagttta agagcctgaa ggttgagagc
    11941 tccttaacat caaagtaccc cctacgagcc agcacgctgt accatgctgc cgaaagatgc
    12001 ccgttggaca ggaaaaacag atcttccccg gtgccatcca tagtaaaatg tttaggatcg
    12061 gccctaagca cgtcgaagta cagcgctgtg aaaaaatcgg cacaacccaa ggatccacct
    12121 ggatgtccac ttgatggcct gtgcaccatt cggattacat cgcgccgaac ctgagcagcc
    12181 attttattaa gaaactcgaa atcagtcatc tatttgttaa actttaagaa ctttttccaa
    12241 tatacaaata tagtaatact tattgagagt tagccgttca accataccgg ttgccgtttg
    12301 tatgtatgct ggtgctgcca gctaaacgct aaccccaaaa tcacgtaggg cgttatttaa
    12361 cgaagttttg agatcggttg actccttacg tttaccaata atcagggcac aggcaacctg
    12421 gtagctaccg gcagggaatt gcttggtgta ggaacccgga ataacaaccg agcggggtgg
    12481 aaccattcct ttatactcca ctggttggga acccgacaca tcaattattt tggttgaccg
    12541 ggtgatggcc acgttggcac caagcaccga ttccctccca atgcgaacac cctccactac
    12601 aatgcatcgc gaaccaatga aacacccatc ttcaatgata acaggagccg cctgaacagg
    12661 ttcaagcact ccgcctattc ccactccacc actcaggtgc acgcccttac caacctgggc
    12721 gcatgaacca acggttgccc atgtatcaac catcgtcccc tcatctacat atgcgccaat
    12781 gttgacatac gagggcatca taaccacacc cctggcaata tagcttccat accgtgccac
    12841 ggcatgggga actaccctta cacccagcga ttggtagttc ttttttaggg gtatcttatc
    12901 gtaaaactcc agcgggcctg cccctgtagc ctgaaagcta catatggaaa agtagagcaa
    12961 aacggccttc ttcacccatt cgttaacctt ccacccatca tcagcaggct cggccacacg
    13021 aacttctccc ttgtcaagca tttcaaccac gcttttaata gcatcgttta cctcaggggt
    13081 acctagcatt tcgcgatttt cccatgctgc atctataatt tcctgaagat taaccataaa
    13141 ttgtcagtat ttctgttcaa ccaattctat attgagaagt caaaaagtaa gtattttagt
    13201 aaggttttgc attcagctta aaccttatac gggctgcata taatccataa agtttaaact
    13261 gaaaccaaaa cgtggttcag ctgcaaatgt agctttttta caggcagatt ttgataaggg
    13321 ctgattataa cctaaaaata gaaaattact tggctatgag aaaattgatg ctactttcgg
    13381 ttttattggt agtttcgtta ggcttgcaat cgcaggagct ggttcgctac caagttccgc
    13441 cggcagaaat ggtggccatt ctggaagcac ctcccactcc gggatttttg cttagccccg
    13501 acggcaaaca tgccttgctg tcataccctc aagttctgcc cgacctggcc gagctggcta
    13561 agcccgagct gaagctggca ggcttcaggg ttaaccccga aaactttgga cgttacgatt
    13621 cccgaatatt cgacaggttg gagttgctca atattagtaa caaaatggtt acttctcttt
    13681 cggggatacc cgatagcggt catattacct ctttcagctg gtcccctgac gggaaaaatg
    13741 ttgccataag tgtttatttc aacagctcgg ttgaactttg gatagttgag gttgaaagtg
    13801 gagtagccag gccgctatca gctagtttga acgagtctgt tatttctcct ggatttgcgt
    13861 ggcatcccga tggcaaatcg ctttacgtat tgaagcaccg tgaaggtatg aaaaagcccg
    13921 gcagggaaaa gttgcccttg ggtccatcgg tgcagcaagc tacgggacag aagtctcagg
    13981 tgaggacatt tcaggacttg ctcaaatcac ctgccgacga ggagttgttt gagtactacg
    14041 ccacttcgca gctttacagg gtaaatttac ttggagaggc ttccgaggtg ggtaacccgg
    14101 gcataatttc acaagttgag ccatcgcccg atggtagcta tatctacgtg gagcaggttg
    14161 taaggccatt ttcatacatt gttccggcgc aatattttcc aaggctctgc catatatgga
    14221 acgcggatgg gcatttggta aagcgattag ccgaacaacc tcttgccgat gacataccat
    14281 cgggattcag cgctgctcgt aaggggaaaa gggagatgca gtggcgttcg gatgttcctt
    14341 caaccattgt gtgggtggag gctcaggacg acggcgatcc acaccgggat gttaaaattc
    14401 gcgatgtggt ttactcgctt gatgccccgt ttagaggcga accggtaaaa ttggttgaac
    14461 ttgaaacacg tttctcctca attagctggg gatggagtaa ccttgcggtt gcttacggcc
    14521 gttggtggag tagccgaagg caaaccgctt ccctatttaa tccttcggca ccaaccgata
    14581 ggccaagggt actttgggat aggtcgtggg aggatgccta caccgatccg ggacaacttg
    14641 taatggtaaa taacgctaat gggttcagcg tgctacaaac caacagcagc aggacaaaac
    14701 tctaccttag cggtaaaggc tcttcgcccg agggcgatat gcccttttta gacgaaataa
    14761 acttaaaaaa cctaggcgtg aaaaggctgt ggcaatgcca tgccccgctc tacgagtact
    14821 ttatcggttt ttacgatatg gataactcgc gtattgttac ctcccaggaa tcggttaaca
    14881 ggcagcccaa cttcttcatc cgtgatatca agaggcagaa gctggtacag cttaccaatt
    14941 tccctgaccc atatcctggc ttgcatggag tgcacaagga gctggttacc taccgacgtc
    15001 ccgatggggt taccctgacc gggacactct acctgcccgc tggctacgaa aagggaacca
    15061 gaacgctacc caccctcatg tgggcttatc ctcaggagtt cctaagtgcc gattttgccg
    15121 gacaggtaac cggttcgccc tatcggttta tccggccgtc tgttttgtcg cctatacttt
    15181 gggttgtgcg tgggtatgcg gtattcgata acatgtcaat gccaatagta gccagaaatg
    15241 gcggtgaacc caacgatacc ttcattgaac agctggtaga gaacgcgaag gcagcagtgg
    15301 atacgctggt gggcatggga gtggctaacc cccaaaaggt ggcaattggc ggccattcct
    15361 acggtgcttt tatgactgcc aacctgcttg cccactccga tctttttgca gccggaattg
    15421 cccgcagcgg ggcttacaac agaaccctta ccccatttgg ctttcagaac gaggagcgaa
    15481 cctactggca agcccctgag gtttacaatc gtatgtctcc cttcatgcac gccgataaaa
    15541 ttaacgagcc ccttttgctc attcatggcg aaaacgataa taatagcgga acattcccgc
    15601 tacagagcga acggctgttt gccgctataa aaggacacgg tggaacagcc aggctggtaa
    15661 tgcttccata cgaaagtcat ggctacagag ctaggcagtc ggtttttcac accatttggg
    15721 aaatggattg ctggctcgaa aaatacctga aatgatatga gcctgttaat agatttgatg
    15781 atataattca tggataaagg attcacggtt tatgtttagg agttttctta gcacttttca
    15841 atttttctta cctgtttgga ttttctggaa attcatccca gtaaattgat ggcgttaaaa
    15901 aaagttaacc gaagctgccg catcactttt aataattaaa tattacgcta tttttgggaa
    15961 aaatgatagc ttggttggtt ttcgtaaaat atgggtgctt attttctttg tggttttctg
    16021 caaagtatca ttcagccagg aaaagctact accctttgat acgcttagca ttgttcactt
    16081 cagggttatc aatgcaaaca cccgggaacc tgttgaaaga gcctacatac tgaatttaac
    16141 ctacaactgg ggaagtattt ccgatttgct gggttacagc tatttgccga tacactttgg
    16201 cgataagctt aaaatttctg caattggata ctatactgaa gagttgattt gcgttggcca
    16261 atttaatgcc gattctgcat tttatgagat aaagctaaaa cccagggtgt atgagttgaa
    16321 cgaggttaaa atcaccaagt tttcgaccta cgaaaggttt cttagaggtg tgattaacct
    16381 aaatattcct aagaccaggg atgataagca gctagaggtg attgaaaaat acctgtttgg
    16441 cgttgtaaag aagatggatt taaaaaatac acccaccatg gcttcgggga ttgcttttgg
    16501 taaggattgg tatgccaagc agaatgagaa gttggcagaa cagctggaga gggagaaaac
    16561 ccggagggta atagatgaaa aatttaatcc cgggcttgtc cagaagctta caggcctgag
    16621 tgataaggaa ctttacgagt ttatcggtta tttagacttc agcgatgagt tcctcctgca
    16681 ggctactgat tacgagatac atcaaaaaat aatggaaaag tataacgtct ttatggcggg
    16741 aaaatcaaac gagttaaaaa atcaagaaaa atgagtgagg agaattcaag ctgctgtcaa
    16801 ccagaagctt ccgacgtagt ttcggcctat gagctgataa aggataaggt gaagcatact
    16861 ccggttcatt cgtcggcgag tgtaaatgct cttctgggag caacactctt ttttaagtgt
    16921 gagaactttc aaaaagtcgg agcatttaag tacaggggtg caacccatgc agttttaaag
    16981 ttgaatgatg aggaggctag aaaaggggtt gcaacccaca gttcaggcaa ccatgcggct
    17041 gcccttgcgc ttgctgctgc aaatagaggt attccggcct atattgtgat gcccgaaaat
    17101 gcccctgcta ttaagaaaaa agcagtagcc ggttacggag gtatcattac ctactgccga
    17161 tcaacgcagc aggacaggga ggaaacgctt agggttgttc agcagcaaac cggtgcaacc
    17221 ctcattcacc cctacaacaa cataaacgtt atctgtgggc agggaacagc tgcgttggag
    17281 ctgctcgatg aaatgcccga cctggatgtg gttatagcac cggttggtgg tggcggattg
    17341 ctcagcggca cagcaacctt tataaagggg caatacccgc acgtgaaagt gttcggagcc
    17401 gaaccaatca atgctgatga tgcctttcgg tcgcttcgtg atgggaaaat ttacccttcg
    17461 ctcagtccat ccaccattgc cgatgggctc ctcacctcgc tttgcccgct tacctactcc
    17521 attctccggt cgaagtgcga tggcattttt acggtatccg agaagggtat agtaaacgcc
    17581 atgcggttaa tctgggagcg tatgaagctt gtggttgaac cctcatcagc tgttccggtg
    17641 ggcgttatag ctgagcaccc agaggctttt aggggaaaac gagttggtat aattgtctca
    17701 ggtggaaatg cagatctcga ccatctgccc tggctggatc agtctaatgg gtaagggtaa
    17761 aatttgcaca aacagggtag tggtcggaaa ggttaagacg cgggcttgaa aattttaaag
    17821 ttttgaattc aggcgaatgc aggatgtagt ctatacggta cgaaggtaga atgccggcat
    17881 aggtatttag cattccggca cctgcctctt taaacgaatc atgcagctta cccgtgagct
    17941 ccctgtaggt gtatgaaacg ggggtatcat taaaatcgcc acaaagaata accggataag
    18001 ggcagttttt caggtgccgg ttaaccagct caacctgctg ggaacgcttg atgaaagcga
    18061 ttcttagcct cgaagccaaa tccataattt cgtcataccg gtcattcccc tgccttaatt
    18121 cattatttag cagaaagtta aagttgtgct ccttaagcct gagcgattgc aggtgaaggt
    18181 tgtaaaccct cacggtatcg ccgttgattt taacgtcggc atagatggac gagttgaggg
    18241 tgttctcaaa tttaatttca cccgaattta ctatcggaaa tcttgaaaag attgccagac
    18301 cgtatgccga acggctattt tgaatgatat acccaatctt ggagcgcatg cccagcttcc
    18361 ttttagcatc ggcctccgaa aatttagtgc ttactgtgta aaactcctga aggcaggcta
    18421 tatcgaagtt gttgctgccg atgtagcgaa tcaccccatc atatgagggc ggaagtttag
    18481 accatgggta gagcctgaac aggtttacat tgtatgaaat caacctgatg gaggggacag
    18541 ccgactcatg cttgttgcca gggcgtagct ggacaaaagc gctaaggtga tttattccta
    18601 gcagaatggc gagtagcgaa attaacagct cttttttcca tacaatgatc cagtaggtca
    18661 ggaaggctat gttggcaagc aggaaaaatg ggtagaatag gcctacaatg gcaggtatcc
    18721 aaaaccgggc ggggttgatg taaaccgacg catagcagaa tagcagacct agcgcaaaca
    18781 aaatgtttgc tgcaacaatt actttatgta gaattccgcc cttggccatg ctagttcacc
    18841 gagtcgtttt tcattcgaaa cagggtttcc ttttcctcct tgctcaggct gtcgtagcct
    18901 gatttggcta ttttgtcgag aatccgatcg atagattttt gctcgttggc ctttcggcgg
    18961 ttgtactcgt agtcggtttc gggtcggcgg taggaaacct tgagattttt gctccgatgg
    19021 gctttgggtt ttttcacccg gctgaaaatg ttgagaaatt ttgtcaaatc agttcccctg
    19081 ttaagcagct tgatgaagga gaagccgaat accgctcctc ccaggtgagc gatgtgccca
    19141 ccggggtttg aaaccggaat gctgatgatg tcgatgatta tggtggccag cgccaggtac
    19201 ttcaacttta cttcacccag aaagaggatg taaattgaat tgttgggctt aagggttgaa
    19261 accgctataa caactgccaa aaccgatgcc gatgctccaa gagccatagc ttcggacgat
    19321 atagccctga ataccggtag aatattgtag gctgcgataa aaaatattgc tcccgtaatg
    19381 cctcccagga ggtaaactcc caccagctgc ctttgcgaga gatgctgtag aaacaacctt
    19441 ccaaaccagt acaaccacag caggttgaat agtatgtgca agaaattttc gtggaaaaac
    19501 atgtaggtta gcagcgtcca cggtcgagaa agtagctggt gtaaacctgc gggaacggca
    19561 agccagtggg cgtagctgct gcccggctgt gtgccaccta tgaacatgat taccctaaca
    19621 aggctataga tcaggaagat tgccagatta atgtagatca gctgggtaag gctgctgcca
    19681 aacctgaagg aatcttttat ttctttcgaa atgctcatgt tgtgtagctg gtagtgtaaa
    19741 tttataagtt ttggtcgaaa tatccaagca gcaacttttg agagtggtaa aagctgttag
    19801 catgttaggg ttgcaacaat ggcatatttg ctcataagca aatattttca atttactaaa
    19861 ctataagggt agcccattgg ttgttttcta caatttttca ataactagta gaaaacattt
    19921 tttctcgatt gccagtattt tatcaggaaa aagccaaaaa tcattccacc aagatgggca
    19981 aagtgggcaa tgttacttcc ggcttgcgta agtccaagga acagctcaag tccgccgtaa
    20041 aggataacca tccatttggc tttaatgggg atggggggaa acagaatcat cagaacggtg
    20101 ttggggaaaa gcattccgaa tgcaagcagc actccataaa cagcacccga tgcgccaacc
    20161 gtaggaatat tcagctgcag ggaaagaagc tgttggctta gctgctgggc ctgatcaatc
    20221 ataatcgggt tgcttggatt ctggaaccac ttatctagaa agtcggagta aatactgttg
    20281 tagaactcgg gaaagtgctt ggtaacaaac gcggcgaagg tatcgggcga aagcgtattg
    20341 agcatggcaa tagaatctgc ctgaatagag gagaattgca ggtagttaac aagcgagtga
    20401 acggcagcag ctccaagtcc ggtaaccatg tagtatagca aaaacctttt accgccccag
    20461 acctgttcca gtattctacc gaacatccac agggcaaaca tgttgaaaaa caggtgggta
    20521 aaaccgccgt gcatgaacat gtgggtaacc acctggtagg gtttaaagtg aggcgacccc
    20581 ggtgtgtaaa gccccagcat ggcattcaaa tcgaccccgt aacgttgcaa catccaggta
    20641 accagccaaa ttacagtatt aatcaaaata agatttttta ctaccggggg tatgctgctt
    20701 agaaatccgc ttctacttaa actcattgtc tttaaaaatt taaagggtta aaacccgtta
    20761 caagttaaac atgaaataat gatgaaggtt tttgagctac ttaagccatt tttcaaactc
    20821 gctcatttca ataagagcaa tagttttttt gccgtcggcg gtatagtttg gttccctgca
    20881 ggcaaagagc tggtcaacca cctcctgcat ttccgattcc gagagccgct tgccgtagcc
    20941 cactgcagca gctttagcca tggatagagc aattttctcc tttgcgctgt cgaagggtag
    21001 ggcttcattt tcgttaataa ttgccagcag gttttcgagg aaaagtgcgg ggtcggttat
    21061 ccgcgtgttc ccgggtaagc tgttaatgct tatggtgtta tggtttaaat tgctaatatc
    21121 cagacccagg cgtaggaaat tttcctggtt agtgatgata gtctcaaagt cttgcgggtt
    21181 tagctcgatg gtttgagggt aaagctcgcg ctgggctaca tttttttcaa ggttaagatt
    21241 tttgtagaat tgctcgtaga ggatacgctc gtgtgctctt ttctgatcaa ccaccagcat
    21301 ccccgattta actgcagtga tgatgtactt tcccttaatt tgaaagaacc tgccagtatt
    21361 ttcggcaact gagtcgtcaa aaccctttga ggcgaatgtt tggacggctg tttgtttggg
    21421 ctcaaagtta aagttggggt tgacgtcctg ataaagttgc tcccatcctt taaccggttg
    21481 ctgcggggta aagttgtgtt cccttgtcct ctgggcaaac ctggctcctt catcctcaaa
    21541 cgggttaaaa ttagggttaa gctcaatttc agggagttca accgttgaat ctttggggaa
    21601 atggggaatg ctgaacccag ggtcggagct gaagtcaatg gagggggcca cggcaaattt
    21661 ccccagtgcc tctcttacag ctgcatggac aatctgccat actgcaccat catcctcaaa
    21721 cttaacctcc gttttggtag ggtgaatgtt tacatcaata gaacccgggt caaccctgaa
    21781 gtaaataaag taggagggga tgctgtttgc tggtagcagg cggttgtaag catttaccac
    21841 tgccttgtgg aggaagggac ttttcatgaa cctcccgttg acaaagaaaa actgttcacc
    21901 cgggctcttc cgggcacttt ctggcttacc aatgtaaccg ctaattgcaa caatagaggt
    21961 ttcggcaacg caatcaacca gctgcgagct gatggacttt ccgaaaaggt tcacaatgcg
    22021 ctgcttcagg tttgatgggg caagcttgta aatctccgag ccattatgcg ataggctcat
    22081 ggaaatctcc ggaaaggcaa gggcaacatg ctgaaattca gatatgatat gcttcagctc
    22141 caccgagttg cttttaagga acttccggcg tgccggtacg ttgtagaaaa ggtttttaac
    22201 agtaagagtc gaaccaacag ggcagctcac cggttgttgg gcaacgaatt ccgatccgcc
    22261 tacctgaacc attgagccaa cctcgtcgga caacctgcgt gtacggagtt caacttcagc
    22321 tactgccgca attgaagcca gggcttcgcc cctgaagccc attgttcgaa tgctgtataa
    22381 atcggccgca tccttaattt ttgaagtggc atgacgctcg aagcaaaggc gcgcatcggt
    22441 ttcggacatc ccaataccat cgtccaccac ctgaatcgaa gtttttccac catcctttac
    22501 tatgagctga atggccttgc accccgcatc aatggagttc tccaacaact ccttaactac
    22561 cgaagcggga cgttgaacca cctcgcctgc agcaatctgg ttggcaattg aatcgggtaa
    22621 tagatgtatg atgtctgcca tctgggaaat taccattaaa aaatgaggtg ggctaccagt
    22681 aaaagaattg tcaaaacttc cagaatattc ctggttggta aatagctgtc ggggtgtctt
    22741 atccacatcg ctcccatgcg ctcgggggta aatgccgagt ttgccctatc aaccgaacct
    22801 cgaactattc ctttgtgggt tttaacccag aaaatggtgt ctctgtgctc ttgtgctcca
    22861 aaaaagaaat tatttaccat gccgacctgc cagcctaatc aaacctccaa aattactcat
    22921 tttaatttat tgttttgaga cggttagata ttcacttggg atttgaaagg aaactcaacg
    22981 agcaaaaata gcagatagtt gtttactagc ctgaaatctt taaattcacg aagtgtaatt
    23041 ttggttaaaa ttttgtagct tacacgtagt ttaaaaaatt tcgatattat gaagcccgct
    23101 ggtgctgttg agctgattat cttgactgct gcatccatgc ttaacggttg cacaaagaat
    23161 gttgagccgt ttcctacaag cgctactctt agcccttcgc tttcggttcc gcttggggaa
    23221 ggcggtgtgt cgcttactaa aacccttcaa actcttggaa taccagtggt caatctttca
    23281 gaagatgttc cacaatgggc tacctatgga tttgtttacg ttgccgatac cattcccctt
    23341 aacctgaccg aagtatacaa ccgtggcgat tctattacat acctgatggt tagaacaaat
    23401 atctggaatc aatttccgtt gggtggcagg gcacaggtgt ttttccttga tgctaacaac
    23461 caagttatcg atcagcttta tgaggatatg gtgagtgttg gcccagctga gcatggaagc
    23521 catggcgagg ttgtaaacac tgctttcgaa tcgaacgaaa ccagcttcaa taccagcaga
    23581 atcagcttat taagcgcagc ccaaaaggtg gtaatatatg ccggtttgga aattacagat
    23641 gaaggcgttt ccgaatcgaa tattacctac ttcgaccagt accagcttag ggttcaggtc
    23701 gctttacagg ttgattttat catgaaccta aactagcaaa ttatcgtggc attgcggcga
    23761 atagtcaacc tgctgatttt tttctctgcc tacagcacca ttgtttgtgc ccagcaggac
    23821 aacacgctgt tcctgcttca taatattccc caatcaaact tcaccaaccc ggcagtgcag
    23881 attcagtgcc cggtttatgt gggagttccc ctgctgtcct ctgttcattt caacgtaaat
    23941 tccacgggat tctcgtatgg cgatgttgcc aatgggtcgg gggttgcaaa cctcaacgcc
    24001 ctggttgccg ggatgcaccg gtgggatttt gtttcggagg aggtgcactt cactccagtt
    24061 tcgtttggtt ttatgtacga caggcaccag tacattagct ttgcatggac ggagcgggtt
    24121 gaaacaaagg tgttcctccc aaaaaagttg ctttcccttt cggttgatgg aaatacccag
    24181 tacgttggcg atgagctgaa aaccggcaac ccaggggcga atgccgtgta ctaccgcgaa
    24241 ttttcggttg gttactccaa aaaggtaaat tccgggttga ttctgggtgt tcatgggaaa
    24301 ttattgtttg gcctggccgg tgcgtttacc cgtcgtaggc ctgttaatgt tgatgttgat
    24361 gccattaccc ataacattga tgcccaatgg aatccgaagg tggatgttgc atacccgcta
    24421 ggtgtggcaa ccgatgcaag tggtaacgtt agcaatgtta ccgttggcgg tttttcaccc
    24481 ttgaattttt tcctgaattt tggaaatcag ggaattgctg ctgatttggg ttttatatgg
    24541 cagcatgatg atgttacatg gagcggtagc ttgctggatt tggggtttat ttggtggcac
    24601 aagcagacca cccggttcga gaattcaggt cattttgttt atcgcggcgc tacccctgcc
    24661 gatttggaca atcccgatga ctacatcaac cagctgggcg attccctaaa caaccaaatc
    24721 caggtggaat actcccgcaa aggattcatt acactgctga acccaaaaat ttaccttggt
    24781 gcaagctacc cggtatcgga aaggtttaat gttggagccc atgttcgtgc agagtggtac
    24841 cccggtcgcc ctgttatggg tattacctta tccggaatgt tttttacgaa aaagggtggt
    24901 tcggcagccc taagctactc ggtgatgaat ggttcgttca tcaacgttgg cgctggcctc
    24961 ggttggggtg gccaaacttt tcaattctac gtggtgtccg acaaccttat ggctgcgctc
    25021 tatcccgaaa aggcccgaaa cgccaacctt aggtttggtt ttaacctctt tttcggctgc
    25081 atcgaaaaaa agaagaaggt ggaggtgccc catggcaatg gatgcggctg ctactggagc
    25141 tgggatgata aaaccaggcg aaaacttggt agaaattagc tgctgtccct cttatttggg
    25201 gttgagcgaa ccattttcgt cccggttaca agtaaattga aatttttgcg ggttaatacg
    25261 atactgcaaa agatattttt gcccattgct ttcttaaaac tatttggact gttaaagctg
    25321 accggctaaa aattgtaggg ttctgccttt tgtcttttcc acattttcat tatttttact
    25381 tcgttgaaat ttttaatata acaagtttta aaattttggt tgaaaatcgt aataagctgc
    25441 tgatgaaaaa aatatgccga atatttgcaa ttgcagtatg gatggcccct ttaggtctta
    25501 tggcacagcc ccctgaagta ggtcaggttg ccccggatat cctgctttcc agcccaactg
    25561 gagattccat aagactttcg tcgctaaggg gcaagcttgt gctggttgat ttctgggcat
    25621 cctggtgttc accctgtcgc agggaaaacc ccaacctggt gcaaacctac aacaagttta
    25681 aggacgcctg ctttgagaat ggtaatggat ttaccattta tagcgtttcg ctcgaccaga
    25741 agcaggatgc atggttgaaa gccatagccg acgatggcct ggtttggccc taccaggtta
    25801 gcgatcttaa aggctggcgt agcgaggccg caaagcaata tggcgtgagg gcaattccca
    25861 tgaactacct tatcaacgcc aacggcgttg tggtggctac aaacctcagg ggcgacgagc
    25921 tcgacagcac cttgcggaag ctgcgtaagt ggaggatttt ctccaatgag tgtatttgat
    25981 aatcacatca actagctaac ttttatcgac atgaaggttg gtcatattct gtggaaaaca
    26041 gctaaaataa ttttaattac cctggggtcg ttaatcctgt tggtagttat actacttgca
    26101 gcagctaagc tgtcggaaaa taagatagtt gggctggtgc tcaacaaggt aagcgtggag
    26161 cttaaggcgc cattctccgt cagggaggtt aacctactcc ccttacggaa ttttccaaac
    26221 ctgactgttg aacttcagga ctttagcgtt ggtaaatcaa ccgattcgct tcaatcaggg
    26281 cagagcagca acccttttga tacacttttt agcctgcata agctctacgt tgccgtgaaa
    26341 gctaaaccac ttctgagcag caaggttgta gttgaccagg tggagattga cggggcaacc
    26401 ctgaactatc atgttgactc caccggtgca agcaacttcg atttcctgat gactaccacc
    26461 gataccaccc aagtacctga ggacacctcg caggttgaaa ccgttctgaa tattctcctg
    26521 cacgacctta cactcagcaa cattgttctt aactatgccg acgatagcct gagggctaag
    26581 gcttgtgttg ctattcctga aattaagctc aacggaaaag ttttgggcag ctactatgca
    26641 ggggcagtgg aggggtcaat ttccgtttct gatttggctc ttgagggtac tcatgctgac
    26701 agaatgggta aaacttctct tgacttcaag gtagggtacg ataatggcga tatttctgtt
    26761 gataagctca atttgaacgc cgatggattt gggctgtctg caaatggcaa ggctaaactt
    26821 tccgactcaa tttttgtaga tatgggtgtg ggcctcacca atatggattt tggcaggctg
    26881 actgcctatc tccccgatac tcttctctcc agctacggac tcactgccct tggaggcatt
    26941 ttcgacctta aagcccacat caaaggtttt gtttacgaca ccctcttgct cccctcagtt
    27001 gaggcaagtt tggatttccg aaatggattc atcaaaacca cctcgtaccc cactgtcgaa
    27061 acactagctt tcaacggcaa ggttaagcta cccgacccca acaaccttaa aactgccagc
    27121 gctgatttta gcacattcag ggttaaaaca cacgccagca acctgaacct ggctttcaag
    27181 atattgaata tcgaaaaacc catctacaac gttaagggcg acatgttcat taccctcgat
    27241 gaatttggca gctttattcc cgatagcctt gtcaagagcc tttcgggaaa catcatggcc
    27301 cgcatcgaaa cccgcgggca gctgcccgac agcctgggga tgagcagcgc cgattacttc
    27361 atgcaaaata ctactcttaa tgttaagctt cgcaacataa ccgctgtgct agactcttca
    27421 acctctgtcc gtggtgtaaa ccttaacttc gactttcttc ccggaaagca ggttgctgtc
    27481 gaaaatttca cagccgattt atccattccg ggttaccatg tcggcctcga caattttctg
    27541 ctacgtgcca ggctcattgg cgatattgct aacaccagca agtttggtgt tgatgttgat
    27601 tcactcctca ttaaggttgg aaacagctcc cttggaggta ggttcaaggt aatgaatctg
    27661 gacaaaccca cctattcagc taaatcgttt gtaaatgtta acttcaacga gctcaggcct
    27721 tttattcccg atacgctggt tgaaaccctt acaggacgca tggctgcaac cgtgcagtcg
    27781 catggtaccc tcaacctcga ctcgctttcc agtcaaatta tgcccattgc ttttgagcag
    27841 agccgcttta cgcttaacct cgacagcttg aactttgcca tgcccaacga caccctgatg
    27901 agggttgaca acctctcgct gaagcttgcc atgagcgacg ataccatccg cattaatgga
    27961 tttcatggcc aggctatggg cattgagttt gccattgatt ccacgcgaat ctggaatgtt
    28021 tacaaggcca tgctgaaggg tgagaaggat aaaaagttgg tagccgatac ccgaatacag
    28081 ctcagcgata ttgattttgc catgtttgcc ccattcatgg ctaccgattc cactgctgcc
    28141 gaaccaacac ctcaacctgc tgaacctcag gaaaaagttg cagggtcaga agcgccggca
    28201 accgaaagca acttcaccat tccatcatac ataatcaggg gcacggtggc tgtcaacagt
    28261 tttaagtacg gcaacatgct cattgacaag atatcaacca aattcagggt tgacgacagc
    28321 ctttacgtta ttgacgacct gaagctgaac gcatttggcg gggatcttac cctctcggct
    28381 ctctacgata cccgtaacga tacggccacc gtaattgagg ccaagtgcaa cctcaacaga
    28441 atgaatatca ggcagcttct tgccgataac aacgactttg accaaacctt ctttacccat
    28501 gagaacatcg aaggccttgc caccggtgag ttgtttgccc gggttatcat gcgcgatacc
    28561 aacatcctgt acgacaagat aaacctgctg ggcaacttca agctggagaa tgggggtatt
    28621 tacaacttcg agcccctcat gcagctgtcg aaattcacca acctgaacga gttggagagg
    28681 gttgttttca gaacgctcga gaccagcgta ttcgtttaca acaacgagat ttacttccca
    28741 aaaaccgaca tcgtaagctc agccatggac atttcggttt atggaatgca gagttttgca
    28801 gatgattatg agtaccacct cattgttcat cttggtgacg tccttgttgg taaatccaac
    28861 aagctgctca agcagcaggg aatggagagc gacgtgtttg aaggtcagga caaggcaaat
    28921 cgttcggggc tttacctggt ggcccttaac cgggggaagg attccaagta tggttttgat
    28981 acgcagcatt tgcagcggtt gatgaaaacc accatcaggg ttcaggagcg tgggcttaac
    29041 cttatattca accctaaggt ggttaacttt agcacggaga tagacaggag agaacgcaag
    29101 aagaagccaa atgaagctaa aggttaagct ggacaggcat cacctgattg cctacgccat
    29161 atcgcttgtt ttagttgtgc ttattgggta caagctgtgg cgatatttta cccccgattc
    29221 caaagaggct gtgcttcagg agtcgcccga accaaggcct ctggatatcg tgtttggcag
    29281 cgatacagcc tcccttcccg tttacatgta tgcaagctat agctgcacct tttgcaggca
    29341 gttttttacc gatgtttttc ccatgctgaa aaaggaattt ttagacaacg gcagggttaa
    29401 gctggttatg aggctgctgg agaataccaa ccatcctgac gcgttgaatt ccgcaaaaac
    29461 cgctgtctgc atcaaccggt atggctactt cgacaagctg cacgagttgc tccttaccga
    29521 tagcagggtt atatacaccc ccgaattccg cgacatggtc aacgacttta cagcaaggga
    29581 cgagcttgtg gccgagtgca ttctgggcgg acaggccgac gagtacatat tgcaaattag
    29641 gaacgaattc cgtaaatttg gcctcagcgg tacacccacc tttatcatca accggaatgt
    29701 ttaccggggt taccgcagct accaggagtt caggaagatt atactacatc acatggaaac
    29761 ggaggatagg aagggcataa attaaatgat agccacttgt aaacatggaa atctaagcaa
    29821 acctacagtg tttaacaaac aaaaaattta tggaaatgaa aaacaaaatc tatttgctgc
    29881 cgttgtcgtt gctggctgtt tttacagttg taacctggag ctgccagaag gatgatgaag
    29941 aggatgtttg tcagaagttt gatcctccac aatgcgaaat tgccaatgcc tgctgcccta
    30001 ccgatggggg caactgctac tacgagtatg gcgatcaaaa gttctactgc gacaaaagca
    30061 aggctacagc cgatgacccc gatggttgca atgcagccga agatcaggtt atagatgtaa
    30121 tctgctctac cgccaagggt gctgacatcc agctggccaa ggtgcagcta cgggagctaa
    30181 cccgcaagct gatgaatgag gcccgcatgc tttcggtttg ccattaaccc tgatgggttc
    30241 ccgttgttgt atttgtttgc gcctgtgttt gcaatatttt ctgccgggcg caaacttgta
    30301 tttgaaaggt tgaatacttc aacatttaag ggcaactcat acccaccaaa gttgtgtttt
    30361 taatgccttt ttcaacgtta gtttgaatta aaggccattt aataggctta ctgcctcaac
    30421 gccatgctat aattacagcg tgtagttagt cttaattgtg tagtatggat tttaagcgtc
    30481 acaaaggctt tcagagaaat tcattgcgag tgccagctcg atgtaaaatt ttaccctacc
    30541 cgcattttaa cctttttctg gacgtatttc ttggcagttt ggtagatatt ctcagtatca
    30601 aagccgcatt cggcgtatag ctgctgggga ggaccctgct caatgaactc gtcggggatt
    30661 ccaagcctaa ccaccctaac gttgtagccg ttatcgttaa gccattccgt tacaccgctt
    30721 ccaaaaccac caggtaaaca accatcctca acggttacga cagtcgtaaa ccgcttggca
    30781 acggagcgta gcaggtcggc atccaggggt ttaacaaaac gcatgtcggc atgaaaaacg
    30841 cttatacctt cggcttcaag cttcccggct gcagtagcag caagattacc cacatggcca
    30901 atagtcagga tggcgatgtc ggaaccctcc cgaagaatac gggccttgcc aatagggatt
    30961 tcggtgaatg atttgcgcca gtcaaccatt accccatttc ccctggggta gcgtatggag
    31021 aatgtaccct gcccatcaag ttgggcagta aacatcatgt ttcgcaactc ctcctcgttc
    31081 atgggagccg caatagtgat attggggatt aaccttaagt aggctaagtc gaaagctccg
    31141 tggtgggttg gcccatcatc accaacgagt ccggcacggt caaggcaaaa caccacgtgg
    31201 agattttgaa gggctacatc atgtattacc tgatcgaatg ccctctgcat gaagctggag
    31261 taaatgttgc agaacggtat aaggccgtcg gcagccatgc cggcagcaaa tgtaacagcg
    31321 tgttgctcgg ctatgcccac atcaaatgtt ctttctggca ttttctccat cataatgttc
    31381 atggagcatc cggtggccat agcaggagta attccaacta ttcggtcgtt actttgggcg
    31441 agttcaacca gggtttcacc aaaaacatcc tgataccttg gtggggctgg cttgctggta
    31501 aggaggctga ttcgctcacc ggtgtccttg ttgaattttc cgggagcgtg gaagattgtt
    31561 tggttttgct cggcaggagt atagcctttt cctttaacgg ttatgcagtg caggagttta
    31621 ggacccggaa tatccttcaa atcgtttaaa attcgtatca ggtaggccac atcgtgcccg
    31681 tcaacagggc caaagtagcg aaatcccaaa gattcaaaca ggttactttg cctgagcagg
    31741 gctgttttga tagcattgtc gagtttctgg atagccgaac gtgccttggg gcctaggctg
    31801 tcaaaccttc ccagtaaatc ccaaacatcc ttctttatcc tgttgtaggt tttggaggtg
    31861 gttatgtcga ggaggtactc gtttagcgcc ccaaccccgg ggtcaataga tatatggtta
    31921 tcgttaagaa taaccagcag gttcgacttc aagctaccgc cgttgttaag cccttcaaag
    31981 gccaatccgc cggttagcgc tccatcacca ataatggcaa caacctggtg tgattcgtgt
    32041 tttaactctg aggcaaccgc cattcccagt gcagcagaaa tggaggttga ggcgtgccca
    32101 acgccaaatg catcgtaggg gctctcgctc cgttttggga aaccgcttat tcctttatac
    32161 ctgcgattgg tgttgaaaac atcccttctg cccgtaagaa ttttatgtgc gtaggcctgg
    32221 tggccaacat cccaaattag cttgtcgtcg ggtgtattga atacatgatg aagggcaact
    32281 gtcagctcaa ctacccccag gctggctcct aggtggccgg gattagttga aaccacctca
    32341 acgataaatc tgcgcaactc ctcgctaagc tcaacgagct gctccggttt catccccttc
    32401 agatcgttgg gcgagttaac atggggtaat attctatagt cgctgcccat tgctgctcca
    32461 cttatattcc tttaaccgca aagttaagaa aatgttgctc ttttctgaaa atatttatag
    32521 ggcacacgga gttgctacct gacgagtgaa agggttagct taagtaccat cataattttg
    32581 atgcgttcat catttaggct tatgtaagct tctgcttttt atgtttgatt tatactaatg
    32641 ggttgtccta taacacttta attacagaca gaatatgtta catggggggt tgccctctca
    32701 tcagtattaa aaaatctaaa attatgttaa aaggctggat gcctatggtt ttattcacta
    32761 ccttttgaac acgttcaagg ctcatagaat aaagggatgg cttgaatttt caataaccag
    32821 ttcaggtttt taaaataagt ttttcttata cgaatttgtt cattaatttt ttaagccatg
    32881 agagcagaac ttttaaagaa gagagcatta acccggatgg ttaggctggc ttcaaatcta
    32941 tcggacgaaa agctgattag gatggtggag ctttcgcagc ggcacctcat gagaggggtt
    33001 accgacgaaa gggtgaggtt tacggcagaa aagattaaga agctctttga ggagaaacat
    33061 cccgcaaccg agctggccaa aagcacattc agaagagtat cgaaggagag ccgaaagcga
    33121 atagttgaaa atttctttat caatgcgggg gttaccggaa ggcaaaggca ggaggagctg
    33181 aagcgtgagc tgggatttgg catgccgtgg ttttttgtta ttagcccaac ggcaaagtgc
    33241 aacctcaagt gcaaggggtg ctatgccggg gagtacacca agtccgagga cctttcattc
    33301 gagctggttg accgcatttt taccgaggcc aaagagctgg ggatgtactt tatcaccata
    33361 tcaggtgggg aaccttacat gtggcctcct ctgctcgata ttttcaggaa gcatagcgat
    33421 atgtttttcc aaacctacac caacggcacg ctgttaacta aggatatggc ccaaaagttg
    33481 gcgaagctgg gcaacgtggc accggcaatt tcggtggagg ggtttgagaa ggagacggat
    33541 gcccgtaggg gcaacggtgt ttttcaaagg gtgatggatg ccatggacaa cctgcgtgag
    33601 gccggtgtgg tattcggata ctctgttaca ccaacacggc tcaactccga attgctagtt
    33661 tccgacgagt ttgttgactt gatgattcaa aaggggtgtg tgtttggctg gtacttccag
    33721 tacgtgccaa ttggccgtaa accggatgtt tcgctcatgg caacgcccga gcaaagaaac
    33781 tatattaggc agcgcaccaa ggagataagg aatacaaagc caatttttat cggcgacttc
    33841 tggaacgatg gtccaattgt gggcggatgc cttgccggtg ccaggcacag gggatacttc
    33901 cacatcaact gcaagggcga cgtggagccc tgcgttttcc tgcagttttc ggtggacaac
    33961 attaaggata aaaagctgct tgacgttata caatctccat tcttcagggc aattcaggat
    34021 gcccaaccct attgtgagaa taagaacctt ctcaccccct gcgctctaat tgataatccc
    34081 cgggttttaa gggatgttgt taagcagcat aacgccaggc cttcctacaa cagcagctac
    34141 gaggtgattt ctaatccgga tgtttgtaat tttttagatg cctattcaag ggattacaag
    34201 aaaattaccg atccaatttg ggaaaacgag gttagcttga agtaccaatc ctggaaggaa
    34261 cgcttcagct agcagaaatt ttgtccattc gtaggatgtt cagtgccggc atgcttcggc
    34321 caactccggt tgaggggtat gatttgcccc gaaatgcttt ggggcataaa accccgctac
    34381 tccacgaacc tacccacccg ccggcggaga gcttgccgaa ggcaaccgtg cggcagcaaa
    34441 gggacgtata atttattccg atacgaacaa gttgttcggt taaatatttt gcaaagttaa
    34501 gtagttgata gatagataga aaaattttaa aaaaaattaa ttttttgata caacattgaa
    34561 atttttatgt tacctttact ttgattttga aaaatcgtta atgggttcta cgattttttt
    34621 gaattaaaag tggaaaaatg aaccgagctt gcgagctcaa cgaagttaat ttttagaacc
    34681 cacctta
//
LOCUS       DAOSHM010000025        33815 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_1013_length_33815_cov_21.364455, whole genome shotgun
            sequence.
ACCESSION   DAOSHM010000025 DAOSHM010000000
VERSION     DAOSHM010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 33815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33815
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1013_length_33815_cov_21.364455"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            261..2831
                     /locus_tag="PK839_05620"
     CDS             261..2831
                     /locus_tag="PK839_05620"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HOG72249.1"
                     /translation="METKKDTLMNNQAEQMAATYSFDEAYEASIKYFKGDEMAAKVWV
                     SKYALKDSFGKIFEKTPDDMHRRIARELARIEARYPNPMGEEALYELLKDFRYIVPQG
                     GPMTGIGNKYQIASLSNCFVIGNSGNADSYGGIMKIDEEQVQLMKRRGGVGHDLSHIR
                     PKGSPVLNSALTSTGIVPFMERYSNSTREVAQDGRRGALMLSISIKHPDSEKFIDAKM
                     EPGKITGANVSVKIDDEFMRAAIEGKPYIQQYPVDSPKPKYRQEIDAASLWKKVIHNA
                     WQSAEPGVLFWDTIIRESVPDSYADLGYRTVSTNPCGEIPLCPYDSCRLLAINLYSYV
                     ENPFTPKAKFNFDLFKRHAHAAQRIMDDIIDLELEKIDAILDKINSDPEEEEIKAVER
                     NLWQKIRRKTIEGRRTGIGITAEGDMLAALNLRYGSDNAVDFAVEVQRVLAVEAYRSS
                     VNLAKDRGAFPIYNAQRETNNPFIKRIKAEDEQLYSEMVTYGRRNIALLTIAPTGTTS
                     LMTQTTSGIEPVFLPYYRRRRKVNPNDKNVKITFVDGVGDSWEEFNVFHHKFLTWLEV
                     NGYNTDEVKGYSDDKLQELVKLSPYYGATSGDVDWVSKVKMQGMIQKWVDHSISVTVN
                     LPENATEELVAKVYQTAWESGCKGCTVYREGSREGVLIADRKDAEGDSKDTFPAKRPP
                     VLDCDVIRFKNNREDWVAFVGLYDNRPYEIFTGLTEDDVLPIPKSVIKGKIIKIKDEN
                     GSRYDFQYVDKYGYTNTIGGLSHMFNKEYWNYAKLISGVLRNGMPIPDVVNLVSSLRL
                     DSETINTWKAGVERALRRYIPDGTKAKPGKKCPECGSESLIYQEGCLTCQSCGYSKCG
                     "
     gene            complement(2891..3397)
                     /locus_tag="PK839_05625"
     CDS             complement(2891..3397)
                     /locus_tag="PK839_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HOG72250.1"
                     /translation="MTSCMQLRGDPLVYETLQRLNISFDYYEHPPVPTVEEATKYWKG
                     IDSTHCKNLFLRNHKGDRHYLVILEYTQVLNIHQLERVLNQGKLTFASPQRLLKYLGL
                     TPGSVSPFGLLHDTSHHVQVFIDSQLCQTHHLSFHPNLNTASLVIPTRDFFIFLAWTG
                     NPYELITI"
     gene            complement(3412..3933)
                     /locus_tag="PK839_05630"
     CDS             complement(3412..3933)
                     /locus_tag="PK839_05630"
                     /inference="COORDINATES: protein motif:HMM:NF019542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid pRiA4b ORF-3 family protein"
                     /protein_id="HOG72251.1"
                     /translation="MNTILYIRLTIDNSNPAIYRDIQVKSQTSLEVMHEIIQATFEWS
                     NLHSHYFKDSAGNIIKDKKGILIGSIITTSGSITYVYDYADSWGVRIEVLEARDIMDE
                     NFTPICTDGRRNSPPEESGGIVCYTDTLDMLQLHQNKEWRYPLIAEWLGEDYNPETFN
                     MEAINSRLQNIII"
     gene            complement(4226..4627)
                     /locus_tag="PK839_05635"
     CDS             complement(4226..4627)
                     /locus_tag="PK839_05635"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HOG72252.1"
                     /translation="MNSLTIEPTTISPHVSFDMDKGVFIISGYSRPENVRDFYAPIIS
                     WLELFRDQLLHEQSEGKPISPIIFNFNLIYFNSSSAKFLYDIVMLLDEVQKKGINLTI
                     KWHYSKDDSELREAGEDLSDMAQVPFDFIEV"
     gene            complement(4669..5079)
                     /locus_tag="PK839_05640"
     CDS             complement(4669..5079)
                     /locus_tag="PK839_05640"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HOG72253.1"
                     /translation="MEPLYIEPSEFTPRVFFDPGNTVFEISGFSRPENTVDFYKPILR
                     WLDEYEENVLNKNTEYSKSNMVLNLKMTYFNSATSKYLLDLLMKFMKFHSKGNTVEIN
                     WFYDEDDDEILESGEEISEMINFPFNYIPITPTQ"
     gene            complement(5093..5605)
                     /locus_tag="PK839_05645"
     CDS             complement(5093..5605)
                     /locus_tag="PK839_05645"
                     /inference="COORDINATES: protein motif:HMM:NF038262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SiaB family protein kinase"
                     /protein_id="HOG72254.1"
                     /translation="MKNSEVLISHNGPLSYEEIGFLLNKMTAILEGYNFGITVKKKVY
                     AAMVESLENVYKHQDPIPEDAGHFPKFTLSLDDKYIYLSVSNPLLKTKIPMLEDRLAR
                     VNQLDKNGLKEFYKQIILNGNVSQKGGAGLGIINIAKVTENKLNFTFTEIDGQHAYFT
                     LGIKVAHYTE"
     gene            complement(5948..8098)
                     /locus_tag="PK839_05650"
     CDS             complement(5948..8098)
                     /locus_tag="PK839_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HOG72255.1"
                     /translation="MKKRFLALLTGLLLLTGAMVRADEGMWLLPLLKQLNIDVMQQKG
                     LKLSADDIYQINQSSLKDAVIIFGRGCTGEIVSDQGLILTNHHCGYGAIQQHSTPEHD
                     YLADGFWAKSLQEEIPTPGLTATFLVRIEDVTDQVNAALNPSMTEAERNQAIQAVGKK
                     IASSATAGTHYQASVRSFFGGNQFFLIVNEVYNDVRMVGAPPSSIGKFGADTDNWMWP
                     RHTGDFSVFRVYAGKDNQPADYSPDNVPLKPKRFLPVSIKGLQNGDFAMIMGYPGSTE
                     RYMTSVEVEEMMSISNANRIYIRGIRQDILLKDMLADKAVNIKYASKYSGSSNYWKNS
                     IGMNKSLKRLKVIDVKKAGEAEFTRWVNANPERVQMYGEALNLINEGVTGRKDYLHAT
                     QYIGEALSRGTELMSIAGIAERIVEPLKSGNNEELSKNVAMLKSRATMFYKDYNAPTD
                     RKVAKAMFKIFSENVPVQYQPDIFNTIRTKYKNDFDRFIDEMFDKSMFVDSVKLFAFL
                     SKPNLKTLQKDPAFIAAQSINAKAMELYGLMEPFNQKLQKGQRLFIAGIMEMNKGKAL
                     YPDANFTMRLTYGEIKDYYPMDAVHYDYQTTLDGVMEKEDPDNWEFVVPQRLKDLYQA
                     KDYGRYGVNGKMPIAFISTNDITGGNSGSPVMNGNGELIGLAFDGNWEAMSGDIVFEN
                     NLQRTISVDIRYVLFIIDKYAGATRLVDEMQIVE"
     gene            complement(8169..8774)
                     /locus_tag="PK839_05655"
     CDS             complement(8169..8774)
                     /locus_tag="PK839_05655"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005822775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HOG72256.1"
                     /translation="MGCLRTIHTPGLVEAGCDEAGRGCLAGPVFAAAVILPPDFYDNS
                     IDDSKKLTLKQRLAARQLIQHYALDWAVARVSNEEIDRLNILNASILAMHMAIKALTR
                     ATPQHLLVDGNRFKNFPGIPHTCIVGGDHTYQSIAAASILAKTYRDELMNELSEQFPG
                     YGWRTNKGYPTKLHRRAIAELGPTPYHRLTFALLPQSKTKC"
     gene            complement(8780..9907)
                     /locus_tag="PK839_05660"
     CDS             complement(8780..9907)
                     /locus_tag="PK839_05660"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HOG72257.1"
                     /translation="MRIAVNTRLLIHGKLEGIGWFTYETFSRMAIDHPNDEFFFIFDR
                     PFSREFIFAKNIHPVVLGPPARHPILWYIWFEFSVNRFLKKLKPDIFISPDGYLSLKA
                     KTPQLAVIHDINFYYYPKGLPWLTSLYLNKYFPKFAGKAKRIVTVSEYSKNTLVEAYK
                     VNPQKIDVVYNGASDNFKPLTEIERADQRNQLTDGWPYFVFVGAFSPRKNISSLINAF
                     DMFRRKTNIQAKLVLVGETMFKASSIEAAHHKSKFKDDIIFTGRKGLPELSRIVGAAQ
                     ALIFPSLFEGFGIPVLEAMLCEVPVAASNSTSMPEVAGNAAIYFEPTSDESIAEAMER
                     IWMDTQLRQRLISNARVQRQKFSWEQAASRLYDIIAKTIES"
     gene            10148..10615
                     /gene="rimP"
                     /locus_tag="PK839_05665"
     CDS             10148..10615
                     /gene="rimP"
                     /locus_tag="PK839_05665"
                     /inference="COORDINATES: protein motif:HMM:NF002531.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly cofactor RimP"
                     /protein_id="HOG72258.1"
                     /translation="MISRETIEAIVAEKLEEENLFIVDISISPKNTITIMLDGEKGVS
                     IKSCVAISRLVESKLDRNVEDYGLEVTSAGLGQPFRVVRQYSINIGREVEVLLLDGNK
                     FKGVIVEAGSDNFSIEYSEKVLMDDGKHKQVVQQRKSFPYNVVKSTKAVISFK"
     gene            10640..11878
                     /gene="nusA"
                     /locus_tag="PK839_05670"
     CDS             10640..11878
                     /gene="nusA"
                     /locus_tag="PK839_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HOG72259.1"
                     /translation="MENINLVDTFAEFKELKNIDRPTMMSVLEDVFRATLIKLYGSDD
                     NFDIIINIDKGDFEIWHNREVVEDGKVEDPGRQIALSEARKIDADFEVGEEVTSEVKF
                     ADFGRRAILALRQNLTSRIMELEKNNLYNKYKDRVGDLITGEVYQVWKKEIMVLDDEG
                     NELVLPKTEQIPTDFYHKGDSIRAVVYKVEMKNNTPQIILSRTANAFLERLFELEVPE
                     IFDGLITIKKIVRIPGERAKVAVESYDDRVDPVGACVGMKGNRIHGIVRELRNESIDV
                     INYTNNTQLFIQRALSPAKITSIKFDETTKRAEVYLKPSEVSLAIGKGGYNIKLASQL
                     TGYEIDVYRESEGEDEEDVNLDEFADEIEGWIIDTLKGIGCDTAKSVIEIPFKELVKR
                     TDLEEETVKEILKILKSEFE"
     gene            11939..14917
                     /gene="infB"
                     /locus_tag="PK839_05675"
     CDS             11939..14917
                     /gene="infB"
                     /locus_tag="PK839_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOG72260.1"
                     /translation="MTNDKSIRISKLAREFNVGVSTLVEFLKKKGYHVTPDPNAKVDE
                     DIVNLLTKEFGAEVNIKKESQKVSLKSLRDAKETLSIDEIEKKRDEDEGDSDFSNDEV
                     IIKTTGFVHEKIDDFGMEKPKIDLKVKGKIDLDNLSSQPRSSKKETPVESTTSEVPVT
                     PLKQATKEVATKKESKEDEGAKPERTKVKTPKVVGKIDLDKTSKKKAKEKPEKNSNQK
                     TKEESAIVENEMQPIVEEKLETVPSVQEVIEKPIEVEREEIYRSTVEKLAGPTIKGKI
                     DITAFETKKKQPVASSDAVKNDKKKRRKRIKKDVGRVQLPEAEQQGTPRSNPPLLVKK
                     ADLPVNNKKSNAGGSKKKRPVKQEVDEEAVQRQIKETLAKLTTKGKTKGSKYRREKRE
                     ILNARQQEENQRLELEKKILKVTEFVSVNELASMMSVPVTDVIEVCMNLGLIVSINQR
                     LDAETMTIVADEFGYKVEFVSIELQESVKDQEDDSPEMLKPRPPIVTVMGHVDHGKTS
                     LLDYIRHSNVIAGEAGGITQHIGAYGVELENGKKITFLDTPGHEAFTAMRARGARLTD
                     VAIIVVAADDAVMPQTIEAINHASAAGVPIVFAINKIDKPTADPEKIKGELANLNYLV
                     EDWGGKYQCQEISAKKGTNVDKLLEKVLLEAEMLDLKANPDRKASGTVIESALDKGKG
                     YVATVLVDSGTLHHGDIILAGIYYGHVKAMFNERGVRITEAGPSTPVQVVGLNGAPQA
                     GDYFHVMESDKEARELANKREQLVRMQGLKTKKHITLDEIGRRIAIGNFQELNIVVKG
                     DVDGSVEALSDSLIKLSTPQIQVNVIHKAVGQISESDILLAAASNAIVLGFQVRPSMG
                     ARKLAEKEEIDIRLYSIIYDAINEIRDAMEGMLSPEIKEEITGTAEVLNVFKISKVGT
                     VAGCIVREGKISRSNKVRLIRDGIVVYTGDLESLKRFKDDVKEVSAGFECGLNINNFN
                     DIKVGDIVEGFQEVEVKRKL"
     gene            15192..15713
                     /locus_tag="PK839_05680"
     CDS             15192..15713
                     /locus_tag="PK839_05680"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HOG72261.1"
                     /translation="MTLPTGSYSQEELSWFRAIFDDYYESIRSFAYYKTGSVDLADDV
                     VQEVFLKLWNIRSSVRNDTVKTLLYTIATNLIKNHFKHQRVVYNFQVSKSTQDEAESA
                     DANVRMDDMNRQLQDALASIPDAAREVFLMNRIDGFTYSEIAQRLNLSVKTVEKRMSE
                     AIRLLRNKFGYKL"
     gene            15710..16603
                     /locus_tag="PK839_05685"
     CDS             15710..16603
                     /locus_tag="PK839_05685"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HOG72262.1"
                     /translation="MTTPKEHTKQVSETGHEELMDAIKALQVPPSTRSKEDAWNMIMQ
                     SIATTVQPKETTRINSLRVWLTTAAAAAIVVVSCFSYLWFSRVSVSSPRGVATDVILP
                     DGSTAKLNADSWLKYPKFYSLVGRKLKIEGEVFFTVTPGRSFVVSDDFKRQVEVVGTK
                     FNMATRGSTFKVACFSGSVMVSNPGSERVQLGKGEVAEAAGKTLKVSKDELHIADSPG
                     WTNGEFKFVGVPLSEVLTELERQFNIKVTAMDFNPNGRTFTGYFTKSSLEQALELVAQ
                     PMSLRYEVSSDSTVVKIYSNK"
     gene            16675..19359
                     /locus_tag="PK839_05690"
     CDS             16675..19359
                     /locus_tag="PK839_05690"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOG72263.1"
                     /translation="MVNRFRIAGAVVVFSLLALMAIPHALMGQALITEKFSNAPLVES
                     LNLISKKYAVKVAFDNEALSGYNINARFTNEPVEKVMSRLLKDTNFEYIVIDNVYVIR
                     RKIAVTTDTPKVNPLKFNGVVRDRNSGESLPYASIKVVNTQLGTAANSDGFFSLAQTQ
                     TDSLAKADSLYIRVSYVGYKPIEMKVARFSHTNSPLVVELDRNDLEIKEVKVTDEYSS
                     IVNHGFRPGELVWSSRQVAQVPTFSGIDAIAPLMLLPGVDGTTESLSGLTVRHSAADK
                     NLVTFDGFTLYNIDHFFGSFSSLNSKVVKDIRILRGGFDAQWGGRASSVIEITGKTGN
                     EHNLVVDAGADELSADVSVEGPIGKKLSYVVAARRSFTDYYRSPLYNSLMGVTRPDFS
                     SVQALANTSDADVVKPTFYYYDANAKVSFKPNIRDIISVSGYTSGDKLTYNQNVQNPY
                     VDENADLSTTGVGFRWARQWGSSFYHNLTIGGSSFNLNYVHQDSIMRYRQASQRDTVL
                     KNYDLENVLNDFSLNLLMQFKPDVHNTLEGGLSSNLVNLNYFESYFHNVNGLSIIDTT
                     HSSDNLSALYSGWLQNTFAGGKLKALTIGFRATYHNLSGKFYFEPRLQAIVAPTPNLS
                     LKLAAGIYNQYVNRIVQANSSGVYRNFWIVSDGKLFPVVKSQHLIAGMTWEDKGWLVD
                     VEGYLKYTEGISFIQNVTRRVNGNQFKTTSLLLTLDSRATGVDLLVRKDWSKGQVWAA
                     YSLSRSFNESPKLNGGEEYYALDDHLHELKIAGTFTVGKWHLSASWIYGSPKPWDEIL
                     LTSSLQLSPDYVKNSVRLIPYHRLDAGLAFVQRVDKVEVTAGVKAFNLYDRQNVIAKP
                     FSLSDTPVQDYLAGRALITFQEIIGMDFTPMFYLNLKF"
     gene            complement(19325..20206)
                     /locus_tag="PK839_05695"
     CDS             complement(19325..20206)
                     /locus_tag="PK839_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72264.1"
                     /translation="MRKKYMQFTTTITGEQVENFCKSTADENPIHNQQHMRELGKEIV
                     VPGLYIFSLIVNFLQKNESLNFNTFRIFFGNIVGINEAIDLGFERSPISESQRYLFAR
                     TGADCFTLDNSRSFADIRSNGIGMEERGRHVPITYNKEQVHIFRKLLGLSNAALLSFL
                     FSVSLASPMLISTIRKPVTDVDKEIAMLFNKSVYPEGISPLYQSLEIFRLENSTILEP
                     EGQVDYWVQLEKEKAGKFYAANVSCGQSGKVMYNSRYRLVAVPNKVMLRMVRELKNQG
                     SKKVEEAKTLNSGRTLA"
     gene            20576..21454
                     /locus_tag="PK839_05700"
     CDS             20576..21454
                     /locus_tag="PK839_05700"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HOG72265.1"
                     /translation="MKRFQKIPDNSFERNKTSLGNELFQSFRVPEGIGHDASWESILR
                     SIKSPSISVSPRIPNLIFKVAATLIVLIGLGSMIWLYGLGNVEVSVPYGQLATHVLPD
                     SSQVTLNADTRLTYNRNRWLLQRRVRLDGEGLFKVMKGSRFTVQTETASTSVLGTVFN
                     VFARKGVTRVSCLEGKVKVVAKESKKEAYLTNGMEAKTMGFELSKPDSKKEEFKTSSW
                     TKGEFYYTNTPIGQVFDELERQFDVDLFFDGDTTRTYTGYFKKDNLNSALRLVCIPMD
                     MKWSVNDTLVIINEIK"
     gene            21503..22333
                     /locus_tag="PK839_05705"
     CDS             21503..22333
                     /locus_tag="PK839_05705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72266.1"
                     /translation="MKRLNVIFAASLMMMAAISFTSCQKDDGDTTDPLVTTMQEGDEV
                     DSYFDETMSEVDELTYESDSKSQMDMAVINSMGQGTRTRKTSWEGDIRIDTVTYNNFV
                     NGNARFERVKNGQIIIRTTGHYLGETFIREVSFNNFTINGNLIEGNKRIQKTSQYTYE
                     IQLENGKVTFSDGTTHTRTMTRTRTMVEGYDTPYYIWDDVYTIEGSSTGVNRQGNAYT
                     HQITNALVVRMDCRWIVSGTFNVTVNNNSMSLDYGNGVCDNLAIATINGRAYEVRLRK
                     "
     gene            22461..22664
                     /locus_tag="PK839_05710"
     CDS             22461..22664
                     /locus_tag="PK839_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019151303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOG72267.1"
                     /translation="MHAVVVNQEKCPRNHPCPVVRICPTGAISQKSWNSAPEISADDC
                     LACGKCVTSCGYGAFRFDNAGAK"
     gene            22702..23223
                     /locus_tag="PK839_05715"
     CDS             22702..23223
                     /locus_tag="PK839_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOG72268.1"
                     /translation="MKKLSGYFLSVLLVAAMLSACDGNNPAKGQQESAPAAPTKTGSK
                     ITGTYAVDITREEFLTKILDYTQEPTNVKYLGNKPAIVDFWASWCGPCRIASPILEEL
                     AKEYHGKILVYKVDTQKEQQISAELGIQSIPTFIFFPVDGKPFATSGIASTPEDTRKM
                     FVEIIEKELLGNR"
     gene            complement(23255..23701)
                     /locus_tag="PK839_05720"
     CDS             complement(23255..23701)
                     /locus_tag="PK839_05720"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF025341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HOG72269.1"
                     /translation="MRQTIPFRIVELEPDSYHMVVEASMSGKSVHLIVDTGASRSLID
                     ISSDSGLRVEGVDETLAVGFNTDKISIQLAIFPYIGVGGFTFSDFVAAVTDLDALRSL
                     YSKVAGMDIAGLLGCDFLVRYVSSINFRTKRIYLKQLTAEVKSSQE"
     gene            24412..25335
                     /locus_tag="PK839_05725"
     CDS             24412..25335
                     /locus_tag="PK839_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004334652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HOG72270.1"
                     /translation="MKNSRKDLVRIGVFYDGNYFLHVSNYYNYFHPRKKRLNIAGLHE
                     FIRNLVASEEEADPRNCQVIEAHFFRGRISAQEASQRGNQLYNDRVFDDILMSEGVVT
                     HYLPIRPRGGRREDKGIDVWLALEAFEQTFYKRFNVLVLITSDGDYVPLIRKVNSLGT
                     KVMVLSWDFDYTDDQGREVTTRTSQELIREVTYPVAMHELINSGLKSKDRLTENLFTV
                     QDFGQAKPVEKPVSPEEIKQARVLSLNSGYGFIQYPPNNLFFYYLDVVDGDFDELIEG
                     DMVEFTISKNDRGQDVAKNVRKIPEYNANKW"
     gene            complement(25458..27947)
                     /locus_tag="PK839_05730"
     CDS             complement(25458..27947)
                     /locus_tag="PK839_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005862081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HOG72271.1"
                     /translation="MQGIYPDSFEQKVGFDRVRELVAGRCLCPIGLQHVESVQFSTDY
                     SLISTWLAQVDEMKSICLMEEGFPVDHFYDLTPSLRKIAAQGAWLDESELLKLRTSLE
                     TIRALLSFFKKFTDRYPAIWELAKNVAYYPTVVERINALLDKFGRIKDNASPQLAQIR
                     SAITSKQSSVSKLVQTILRSARDQGIIEQDASPSVREGRVVIPVAAANKRKLKGIVHD
                     ESSTGKTVFIEPVEVVELNNEIRELEYDERREVVRILVEFSDSIRPYLPELLGAYDFM
                     GMVDFIRAKALFAIEYQGAKPIIKETTGTYLRQAKHPLLQLSLKREGKEIVPLDIELK
                     SEERILLISGPNAGGKSVCLKTVGLLQYMLQCGFLPCVLENSEMGLYRKIFIDIGDEQ
                     SLENDLSTYSSHLLNMKYFLKNATPETLILIDEFGSGTEPSAGGAIAEAILQGFANTG
                     TYGVITTHYSNLKHFAASTRGIVNGAMLFDSSRIEPLFKLEIGKPGSSYAFEIARKIG
                     LPDSIINEATAKVGDDYVTFEKHLREISRDKRYWEKKRENIRLASKKAEAEEAQLTRE
                     LEELASQRKEILAAAKKNAQELLAETNRKIENTIRIIKESNAQKEETRKARQELEQFK
                     QQVDEAAHNLNDEVERKMERIKQKQEKRALHRKAKEPAASSRTEPVASPPLTIGDRVK
                     IIGSDLLGEVVGLSGQNVSVAVGNLISMVNLERVQRISTAEFRNANKAVTQPQVGMGF
                     DTYKRRLNFKSDIDIRGARADEALETVQNLVDDALMLGFGRVRILHGKGNGILRSVIR
                     DYLKSTPGVKAFSDEQEEFGGAGITVVDLDV"
     gene            complement(28097..28675)
                     /locus_tag="PK839_05735"
     CDS             complement(28097..28675)
                     /locus_tag="PK839_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HOG72272.1"
                     /translation="MEKSVKGTETEKNLLKAFAGESQAKNRYTFFSKVAKEEGFEQIA
                     EIFMTTALQEEQHAKQFFRFLEGGMVEITASYPAGVIGTTAENLKAAAMGEHEEWSEL
                     YPHFADIAEKEGFTRIATAFKMVAKVEVEHEKRYKRLLEKVEKDKVFERDEEIEWVCR
                     KCGYVHKGKKPPKNCPVCQHPQAYFEEKANNY"
     gene            28954..30309
                     /gene="gdhA"
                     /locus_tag="PK839_05740"
     CDS             28954..30309
                     /gene="gdhA"
                     /locus_tag="PK839_05740"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HOG72273.1"
                     /translation="MKNIALQEKVNAFMASLTAKNPGETEFHQAVREVVESLMPFIDE
                     NPIYSDEKILERITEPERVIIFRVPWIDDRGNLQINRGYRIEMNSAIGPYKGGLRFHP
                     TVNLSILKFLAFEQVFKNSLTSLPMGGGKGGSDFDPKGKSDKEVMRFCQSFMTELYRH
                     IGPNTDVPAGDIGVGGREIGYLFGQYKRIVNEFTGVLTGKGLKWGGSLIRPEATGYGN
                     VYFAQEMLKTRGQSIEGKIVTVSGSGNVAQFTVEKAVELGGKVVTLSDSDGFIYDSEG
                     INTEKLAYVKWLKNDKRGRIKEYADKYKCEYYPAERPWSIKCDIALPSATQNEINGEE
                     AKTLVRNGCMCVSEGANMPSTPEAIEVFLNAKILFGPGKAANAGGVATSGLEMSQNAL
                     RLSWTREEVDNRLRLIMSNIHETCMKYGKADDGFINYVNGANIGGFVKVADSMIAQGA
                     V"
     gene            30746..32287
                     /locus_tag="PK839_05745"
     CDS             30746..32287
                     /locus_tag="PK839_05745"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF006942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HOG72274.1"
                     /translation="MNVGIPKELLQHELRVAATPKTVVRLKKQGFEVLVESGCGVRAK
                     FSDEEYVATGAEVVSSAQELYSSSDIILKVQPPTEAEVGLMKRGTLLLSYLFPAQNLP
                     LLGKLAEAGINAIAMDAIPRISRAQKMDVLSSMANISGYRAVVEAANYFGRFLNGQIT
                     AAGKVEPAKVLVIGTGVAGLAAIGTAKSLGAIVRAFDTRKEAREQIQSMGAQFITVEI
                     DEDGSTASGYSKEMSQAYIDAEMALFKRQAAEVDIIITTAQIPGKAAPKLLLEEHVMA
                     MKPGSVIIDLAAPTGGNCVLTKKDEVYTTPNGVVIAGRVDTLPTQASFLYGNNLCNLL
                     DDMGKAENFRIDMNDDVIKRAMVTYNGKVNWPPEPLAVSAQAKKTEAGKPSPEEEAAK
                     KAGKVKKALISNVIWLAVVGALLFLLGKVAPADFMGHITAFVLAVFVGWNVIWNVTHA
                     LHTPLMSVTNAISGIIIIGGLLQIQNDFSDPRTIMAFIAILIAFINIVGGFTVTYRML
                     KMFKK"
     gene            32295..33683
                     /locus_tag="PK839_05750"
     CDS             32295..33683
                     /locus_tag="PK839_05750"
                     /inference="COORDINATES: protein motif:HMM:NF014307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HOG72275.1"
                     /translation="MISTNIQIAAYLFASILFILSLSGLSSQESAKKGVIYGIVGMAI
                     ALCATLFGGNIQGYVYIIVAMVVASVIGVIVARKVQMTAMPQLIALLHSFVGLAAVLV
                     GFSSFIDPKTVSMAGAERAIHMVEIFVGIFIGAITFTGSIIAWGKLTENITSKPLVFK
                     GKDVVNVVLLLASVVLGVMFVGQGGTHGMPFLIAMTVIAAFFGVMLVMAIGGADMPVV
                     ISMLNSLSGWTAAASGFMLGNDLLIVTGALVGSSGAILSIIMCRAMNRSFFAVISGGF
                     GQEVKTGRKLEGKVNSMTHDEAAEWLKEAQSVVIVPGYGMAVAKAQYPIHDMVEKLRS
                     QGKSVKFGIHPVAGRLPGHMNVLLAEANVPYDIVLEMDEVNPELPETDVAMVVGANDT
                     VNPGAQEDPSSPIYGMPVVEVWKAKRVIVMKRSMAVGYAGVENPLFYKENVSMLYGDA
                     KDSVDKINAKLG"
BASE COUNT         9307 a         7162 c         8104 g         9242 t
ORIGIN      
        1 tccttgtctt cagccccaac ggggcgcaat agctagcaag gggtgaagtc ccttgtcagc
       61 ttttattgcc ttcccttcat aagctgccgg tgttaataac taaaaatgat tgttaatatt
      121 tagaacatgt ttagcttggc gtcaaaggtt aacctttaat aacttcgtac ctgctttccc
      181 aaattttcaa aaatccgcac aattcttatt gtcaggcata tgggaaagtt ttttattgcg
      241 acttatacaa ccaaaatata atggagacaa aaaaagatac cctgatgaac aatcaggcag
      301 aacaaatggc agcaacctat agttttgatg aagcctacga ggcaagtatt aagtatttta
      361 agggtgacga aatggctgca aaggtatggg ttagcaagta tgcccttaag gattcctttg
      421 gtaaaatttt cgaaaagaca cccgacgaca tgcatcgtag aattgcccgt gagttggcaa
      481 gaatcgaggc aagataccca aacccgatgg gggaggaagc gctctacgag ctgctaaaag
      541 attttcggta catagtaccc cagggagggc caatgaccgg cataggcaat aagtatcaaa
      601 ttgcctcgct ttctaactgc tttgtaatag ggaatagcgg taatgccgat tcgtatgggg
      661 gaattatgaa aattgatgag gagcaggtgc agcttatgaa gcgcagggga ggtgtaggcc
      721 acgacctttc ccacatccga ccaaaaggaa gccctgtgct taacagtgcg ctaacctcta
      781 caggtattgt gcccttcatg gagcgctact caaactccac gcgcgaggtg gctcaggacg
      841 ggcggcgcgg agctctaatg ctaagcatct ccataaaaca tcctgattcc gaaaaattta
      901 tagatgccaa gatggagccc gggaaaatta cgggtgctaa cgtgtcggtt aaaattgacg
      961 atgagtttat gcgggcagct attgagggta aaccttacat tcaacagtac cccgttgact
     1021 cacctaagcc aaagtaccgc caggaaattg atgctgcatc cctgtggaaa aaggtgatac
     1081 acaacgcatg gcaatcagct gaaccggggg ttctattctg ggataccatt attcgcgaat
     1141 cggttcccga cagctatgcc gatttaggtt atcgaaccgt atcaaccaat ccctgtggcg
     1201 agattcccct ctgcccttac gatagctgcc ggttgctggc aatcaacctt tatagctatg
     1261 ttgaaaatcc ctttacccct aaggcaaaat ttaacttcga cctgttcaaa aggcatgccc
     1321 atgcagccca acgcatcatg gacgacatca ttgacctgga gctggaaaag attgatgcca
     1381 ttctcgataa aattaactcc gaccccgaag aagaagaaat taaggctgtt gaacggaacc
     1441 tctggcagaa aattaggcgc aagacaattg agggaaggcg aactggaatt ggtattacgg
     1501 ccgaaggaga tatgctggct gcactaaact tgcgctacgg ctccgataat gcagttgatt
     1561 ttgctgttga agttcaacgt gtgctagctg ttgaggcata ccgttcgtct gtaaacctgg
     1621 ctaaggatag gggagcattc cctatttata acgcccagcg tgaaaccaac aaccctttta
     1681 ttaagcgtat taaggccgaa gatgagcagc tttatagcga aatggttacc tatggcagga
     1741 gaaatattgc cctgctcacc attgcaccta ccggcacaac cagccttatg acccaaacaa
     1801 cctctggaat agagccggta tttctgccct actacagacg acgccggaag gtgaacccca
     1861 acgataaaaa tgttaaaata acctttgttg atggggtggg ggattcatgg gaggaattta
     1921 acgtgttcca tcataaattt cttacatggc tggaggtaaa cggctacaac accgatgagg
     1981 ttaaaggtta cagcgacgac aagcttcagg aacttgttaa actatcaccc tactatggcg
     2041 ctacttctgg agatgtggat tgggtaagca aggttaaaat gcaggggatg atacagaaat
     2101 gggttgacca ctccattagc gtaaccgtca acctgcccga gaatgccacc gaagagctgg
     2161 tggccaaagt ttaccaaact gcttgggaaa gcggttgcaa gggttgcact gtctatcgtg
     2221 agggatcgcg tgagggcgtt ctgattgccg atagaaagga tgccgaaggg gattcaaaag
     2281 ataccttccc tgctaaaagg ccacccgtgc tcgactgcga tgtaattaga ttcaaaaata
     2341 accgtgagga ctgggtggca tttgttggct tgtatgacaa ccgcccctac gagattttta
     2401 ccggtttaac cgaggatgac gttctcccaa tccccaaatc ggtgatcaaa gggaaaatta
     2461 tcaagataaa ggatgagaat ggaagccggt acgactttca gtatgttgat aagtatggct
     2521 ataccaatac gattggcggc ctgtcgcaca tgttcaacaa ggagtactgg aactatgcca
     2581 agttgatttc aggtgtgctg cgcaacggaa tgcccattcc cgatgtggtt aatcttgtgt
     2641 cgtcgctgcg gctggatagc gaaacaatta acacatggaa ggcaggagtg gagagagccc
     2701 tacgtcgcta cattcctgat ggcaccaagg ccaaacccgg caaaaaatgc cccgagtgcg
     2761 gttccgaatc gctaatttac caggagggat gtttgacatg tcaaagttgt gggtattcca
     2821 aatgcggcta agttgtcacg tgtagctatt taagctcgtg ttccccttgc tagtgtgcag
     2881 ggggaaattt ttatatggtg ataagctcgt aggggttacc tgtccatgcc agaaagataa
     2941 aaaaatccct tgttgggatt accagggagg cggtgtttag gttagggtga aagctgaggt
     3001 ggtgagtctg gcacagctga gaatcaataa acacctgcac atggtgtgag gtgtcgtgga
     3061 gtagtccgaa aggtgaaacc gagcctggag ttagccctag gtatttgagt aagcgttggg
     3121 gtgatgcaaa cgttagtttt ccttgattta gcaccctttc gagctggtga atgttaagaa
     3181 cttgtgtgta ttcaaggatt accaggtagt gcctatcgcc cttatgattc cgaagaaata
     3241 ggtttttaca atgggtggaa tcaataccct tccagtattt tgttgcctcc tcaacggttg
     3301 gcaccggagg gtgctcatag taatcaaaag aaatgttaag cctttgcagg gtttcgtata
     3361 caagcggatc gcctcgaagc tgcatgcacg atgtcattgg tggtggacct tctaaattat
     3421 aatattctgc agcctgctat tgatcgcctc catgttaaag gtttcgggat tgtagtcctc
     3481 gccaagccat tcggcaatta atgggtaccg ccattccttg ttctggtgca gctgaagcat
     3541 atccagggta tcggtgtagc aaacaatacc tcccgattct tcaggagggg agtttcttcg
     3601 accatcggtg catatcggag taaaattttc gtccatgatg tcccgggctt ccagcacttc
     3661 tattctgacc ccccacgaat cggcataatc gtacacatag gtaatacttc cgcttgtagt
     3721 aataatgctg ccaatcagaa tacctttctt gtccttaatg atgttgccag ctgaatcctt
     3781 aaagtagtgc gagtgcaggt tgctccattc aaatgttgcc tgtatgattt cgtgcatcac
     3841 ctccaggctt gtttggcttt ttacctgaat gtccctgtaa atggcaggat tggagttgtc
     3901 aatggttaat cgaatataaa gtattgtatt catatcgggg tagtaaatca tttattcagc
     3961 attcttccaa aaatgtcttt tatatagata cccagcaaag taaactaaaa gtaagaatat
     4021 tttcaggaaa aattgtaggg gtgattaaaa cctgttcaag ggctgaaaaa taatcaggtt
     4081 ttgatttatt agtcgaaggc cttcttgtat tgttgttcat actattgcgg gtagcagtaa
     4141 cgcttaggag tttttacaag atggtttgat tggtgggaat taaaacgaaa acatccatat
     4201 ttcgctatgg agtggataaa ttaacctaca cctcaataaa atcaaatgga acttgagcca
     4261 tatccgagag atcttcacca gcctcacgga gttcggagtc atctttactg tagtgccatt
     4321 taatggtgag attgatgccc ttcttttgaa cttcatcaag gagcattacg atatcgtaaa
     4381 gaaattttgc cgatgaggag ttgaaataaa taaggttgaa gttgaaaata atggggctta
     4441 ttggtttccc ttctgattgc tcgtggagaa gctgatccct gaataattca agccatgata
     4501 taattggtgc atagaagtcc ctgacgtttt cgggacgtga atatcccgaa atgatgaata
     4561 cccccttatc catatcaaag ctaacatggg gtgaaatagt agtgggttca atggtaagag
     4621 agttcatgaa taaacctgtt tttaaaagat ttggttatta ataagaagct attgggttgg
     4681 ggttattggg atatagttga agggaaagtt tatcatttcg gaaatttctt cgcccgattc
     4741 aaggatttcg tcatcgtcct cgtcgtagaa ccagtttatc tcgaccgtat ttccctttga
     4801 atggaacttc atgaatttca tcagcaggtc gagcaagtac ttggaggttg cagagttaaa
     4861 ataggtcatt ttaagattaa gaaccatgtt gcttttgcta tactcggtgt tcttgtttag
     4921 tacattctcc tcgtattcat cgagccatct aagtataggc ttgtaaaagt caactgtatt
     4981 ttcgggcctt gaaaaccctg agatctcaaa tacggtgttg ccagggtcaa aaaagactct
     5041 gggtgtaaac tcgctgggtt ctatgtatag tggttccata gtttttttcg aattattccg
     5101 tgtaatgtgc aacctttata cccagtgtaa aataggcatg ctgcccgtca atttcagtaa
     5161 aagtaaagtt tagcttgttt tcagtaacct tggcaatgtt gatgatgccc agcccggcgc
     5221 cacccttttg tgaaacatta ccgttaagga taatttgctt gtagaactct tttagcccgt
     5281 ttttgtcgag ctggttgacc ctcgccagcc tgtcctcaag catcggtatt ttagttttga
     5341 gcagcgggtt cgaaacgctt aggtatatgt acttgtcgtc caacgagagg gtaaactttg
     5401 gaaaatgccc tgcatcttcg ggaatagggt cctggtgctt gtacacgttt tctaaacttt
     5461 ccaccatggc tgcataaacc ttctttttta cggtaatgcc aaaattgtac ccttccagga
     5521 tggctgtcat cttattgagc aagaatccaa tttcctcgta ggaaagtggc ccattatggc
     5581 tgatgagtac ttcgctattt ttcaccttat aatgctacaa aaattttact aaattatgaa
     5641 atcgaacttt aaaattgcaa ttattaccca aaaatacttt tgttaaaaaa aagtagctag
     5701 tgaaatatag ggagttaggt tgcatgcaaa acatcaagtt taaggcagcg taacctaccc
     5761 ttatttgcca accaatattc attttaatat tttacatacg gaaaatattt ttgacgagtg
     5821 gaggaatttt gtaattaaat ggttttgccg aatggttata tggcggtttg atgatgaaaa
     5881 ggggatgcca aatactggca tcccctttgc tattattatg ggtttaaaac cgataggaaa
     5941 ttctgtgcta ctccacaatt tgcatctcgt ccaccagcct ggttgcaccg gcatacttgt
     6001 caattatgaa tagcacgtag cggatatcaa ccgaaattgt tcgctgcagg ttgttttcga
     6061 atacgatatc accgctcatg gcctcccagt taccgtcgaa tgccaaacct atgagttcgc
     6121 cattaccgtt cattacaggg cttccggagt ttccaccggt gatgtcgttg gttgaaatga
     6181 aggcgatagg cattttgcca tttacgccgt agcggccata atccttagcc tggtataggt
     6241 ctttaagacg ttgcggcact acaaattccc agttgtccgg atcctccttt tccattacgc
     6301 catcgagagt agtttggtag tcgtaatgaa cggcatccat ggggtagtaa tcctttattt
     6361 cgccgtaggt aagcctcatg gtaaagttgg catcggggta gagcgccttg cccttattca
     6421 tctccataat tccagctatg aagagtcgct gccctttttg cagcttttgg ttgaagggtt
     6481 ccattaaccc gtaaagctcc attgcctttg cgttaattga ttgggcggca atgaatgcgg
     6541 gatctttttg aagggttttc aggttcggtt tcgacaggaa ggcaaatagc tttacagagt
     6601 caacaaacat ggatttgtcg aacatttcat ctataaacct atcaaaatcg ttcttatact
     6661 ttgtacgaat agtgttgaag atatcgggtt ggtactgtac aggaacattt tcggaaaaaa
     6721 ttttgaacat agccttggcc acctttctat cggttggggc gttgtagtcc ttgtagaaca
     6781 tggtagccct actcttaagc atagctacat ttttgcttag ctcctcgttg tttcccgatt
     6841 ttaagggttc aacaatacgt tcggctattc ctgcaatgct catcagctcg gttccacggc
     6901 tgagtgcttc acctatgtat tgggtggcat ggaggtagtc ctttcgaccg gtaactcctt
     6961 cgttaatcag gtttagcgct tccccgtaca tttgaactct ttcagggttg gcatttaccc
     7021 accgggtgaa ttcggcttca ccggcttttt taacgtcaat cacctttagg cgtttgaggg
     7081 acttgttcat gccgatggag tttttccagt agttggacga accggagtac tttgaggcat
     7141 acttgatgtt aacggctttg tcggccagca tgtctttcag aagaatgtcc tggcgaatcc
     7201 ctctaatgta gattctgttg gcattggaga tgctcatcat ctcctctact tccacggagg
     7261 tcatgtagcg ttcggtgctg ccggggtaac ccataatcat ggcaaaatcg ccattctgta
     7321 atcctttaat tgaaacagga aggaatcgct tgggtttcaa gggtacgttg tcgggtgagt
     7381 agtcggctgg ttggttatcc ttgccggcat aaacgcggaa tactgagaaa tcaccggtat
     7441 ggcgtggcca catccagtta tcggtgtcgg caccaaactt acctattgac gagggaggag
     7501 cacctaccat tctgacgtcg ttgtaaacct cgttaacaat cagaaagaat tggttgccgc
     7561 caaagaaact tctaacgctg gcctggtagt gcgtaccggc tgtagcgctg gatgctattt
     7621 ttttgcctac agcttgaatg gcctgattgc gttcagcctc ggtcatgctg gggtttaggg
     7681 cagcatttac ctggtcggta acatcctcga tacgtactaa aaatgttgcg gttagccccg
     7741 gggtgggaat ttcttcctgc aacgactttg cccaaaatcc atcggcaagg tagtcatgct
     7801 caggggtgct atgctgctgg atggccccgt agccgcagtg atggttggta agaatcaacc
     7861 cctggtcgga tactatttcg ccggtgcaac ctcgaccgaa gatgattaca gcatctttaa
     7921 ggctcgactg gttaatctgg tagatgtcgt cggcagagag cttaagccct ttttgctgca
     7981 tgacgtcaat gttcagctgc ttgagcaggg gcagaagcca catcccctca tcggccctga
     8041 ccattgcacc ggttaaaagc aacaaaccgg ttagtagtgc aagaaatctt tttttcatgt
     8101 tgtttgagtt ttggttgtta atggttaagt atagtgttat gacagactag ctcttgttta
     8161 gtttaaagct aacattttgt cttggactgg ggcaaaaggg cgaacgtcag ccgatggtag
     8221 ggcgttggcc ctagttctgc aattgccctc ctatgcagct tggttgggta gcccttgttt
     8281 gttctccagc cataacctgg aaactgttct gagagttcat tcattagctc atcgcggtat
     8341 gttttagcaa gtattgatgc tgcggcaatt gactggtagg tgtggtctcc acccactatg
     8401 caggtatggg gtattcctgg aaaattctta aacctattac catcaaccag taaatgctgg
     8461 ggtgttgccc tggtaagagc ctttatcgcc atgtgcattg ccagaatgga tgcattaaga
     8521 atgttcaacc tgtctatttc ctcgttcgac acccttgcca cagcccaatc taatgcgtag
     8581 tgttgaatta gctgccgggc ggcaagccgt tgctttaggg ttagcttttt tgaatcatca
     8641 atggagttat cgtaaaaatc gggaggcaaa attactgctg ccgcaaaaac aggccctgcc
     8701 aggcaacccc ttccggcctc atcgcaaccc gcttcaacga gtcccggcgt gtgtattgtc
     8761 ctcaaacatc ccattcccat caactttcga ttgttttagc aatgatgtcg taaagcctgc
     8821 tggcggcttg ctcccagctg aacttttggc gctgcaccct tgcgttggag atgagtctct
     8881 gccttagctg ggtatccatc catatacgct ccattgcttc agcaattgat tcatcactgg
     8941 ttggttcaaa gtatattgcg gcattccctg ctacctcggg cattgaggta gaatttgatg
     9001 cggcaacagg aacttcacac agcatggcct ccagaacagg tatcccaaaa ccctcaaaaa
     9061 gcgacggaaa aatcagggct tgagcagcgc caacaatacg gcttaactcg ggcaaccctt
     9121 ttcggccggt aaaaataata tcatccttaa atttactttt atggtgggct gcttctatgg
     9181 aagaagcttt gaacattgtt tcacccacca gaaccagctt tgcctgaatg tttgttttac
     9241 gcctaaacat atcgaaagcg ttaatgaggc tgctaatatt ttttcttggg ctgaatgcac
     9301 caacgaaaac aaagtagggc catccatcgg taagctggtt gcgctgatcg gctcgttcta
     9361 tttcggttag gggtttaaag ttatcactag caccattgta aactacatct attttttgcg
     9421 gatttacctt gtaagcctca acaagcgtat tttttgaata ctcacttacg gtgacaattc
     9481 gttttgcctt acctgcaaat tttgggaaat acttgtttag gtacaacgag gtcagccatg
     9541 gcagtccctt ggggtagtag taaaagttga tgtcgtgaat aactgccagc tggggtgtct
     9601 tggctttaag cgacaggtag ccatcgggag aaatgaaaat atcgggtttc agctttttca
     9661 aaaaccgatt tacggaaaac tcaaaccaga tataccaaag aataggatgt cgtgcaggcg
     9721 gaccaagcac aacagggtga atatttttgg caaaaataaa ctcccggcta aacggccggt
     9781 caaaaatgaa gaaaaactca tcgtttggat ggtctatggc catgcggcta aaggtctcgt
     9841 aggtaaacca tcctattcct tcgagttttc catgaatcaa gagccttgtg ttaaccgcaa
     9901 tgcgcatgtt ttctttttgg ctaaatacac aaaaatttta tcaatttgca agttaaggca
     9961 aagtcatcat aacatttgaa aacgttgcta gttgctatgc tggtgcgcac tttaaataga
    10021 actaacccga taaaattcat aaattcagca taatattttg aaagaatgtt agaaagctgt
    10081 atatttgcaa cagtttttaa gcaggtggca ggggacagag tcccctgttt tattagatgt
    10141 agcaataatg attagccggg aaacaattga agcgatagtt gcagaaaagc tggaggagga
    10201 aaaccttttt attgttgata tctccataag tcccaaaaat accataacca tcatgcttga
    10261 tggtgaaaag ggtgttagta ttaaatcatg cgtggcaatc agccgtttgg tcgagtcgaa
    10321 gctcgatagg aacgttgaag attatgggtt agaggtaacc tctgcagggt taggacagcc
    10381 gtttagggtt gtccggcagt atagcatcaa tattggcagg gaggtagagg ttttgctttt
    10441 ggacggaaac aagttcaaag gcgttattgt ggaggctggt agcgataatt ttagcatcga
    10501 gtatagcgag aaagttctta tggatgatgg taagcacaaa caggtggtgc agcagcgaaa
    10561 gtcgtttccc tacaatgtgg taaagtcaac caaagcggtc atttcgttta aatagtaaat
    10621 atgataaata atcagcacaa tggaaaacat taacctagtt gacacatttg cggagtttaa
    10681 ggagttaaaa aatattgaca ggcctaccat gatgagcgtt ctggaggatg tttttagggc
    10741 gacgcttata aagctttacg ggtctgacga taacttcgat attattatca acattgataa
    10801 gggcgatttt gaaatttggc acaaccgcga ggtggttgag gacggcaagg tcgaagatcc
    10861 aggaaggcag atagccctat cggaagctag aaaaattgat gcggactttg aggtgggcga
    10921 ggaggtaacc tccgaggtga agtttgcaga cttcgggcga agggcaatcc tggcattgcg
    10981 ccaaaacctc acctcacgaa tcatggagct cgaaaagaac aacctctaca ataagtacaa
    11041 ggatagagtt ggagacctta tcaccggtga ggtttaccag gtctggaaga aagaaatcat
    11101 ggttctggac gatgagggta acgagctggt gctgcccaaa acagagcaga taccaaccga
    11161 tttctaccac aagggtgact caataagggc cgtggtgtac aaggtggaga tgaaaaacaa
    11221 caccccgcaa attatccttt ccagaactgc caatgccttc ctcgagcgcc tattcgagct
    11281 ggaggttcct gaaattttcg atggcctgat taccatcaag aagattgttc gtattcccgg
    11341 cgagcgcgcc aaggttgcag ttgagtcgta cgacgatagg gttgaccccg ttggcgcttg
    11401 tgttggaatg aagggaaacc gcatacacgg tatagttcgg gaacttcgca acgagagcat
    11461 cgacgttatc aactacacca acaataccca gctgttcatt cagcgcgccc taagccccgc
    11521 taaaatcacc tcgattaagt ttgacgaaac aaccaaacga gccgaggttt accttaaacc
    11581 aagcgaggtt tccctggcga ttggtaaggg cgggtacaat attaagctcg ccagccagct
    11641 caccggctat gaaattgatg tttaccgcga atcggaagga gaggacgagg aagatgtgaa
    11701 tctcgatgag tttgctgatg aaattgaggg gtggattatt gacaccctaa agggcattgg
    11761 ctgcgacacc gccaagagcg tgattgaaat tccctttaag gagctggtga aacgaaccga
    11821 tttggaagag gaaacggtca aggaaattct taaaatatta aaatccgaat ttgaatagta
    11881 ttgtctttca tgtagcttta cacgagcatt tctaacataa acaacgggaa ccacctgtat
    11941 gacgaacgat aaatcaatac gaataagtaa gctggcaagg gaatttaatg tgggggtttc
    12001 tacacttgtg gaatttctca agaaaaaggg ctaccacgtt acccccgatc caaacgctaa
    12061 ggttgatgag gatattgtta acctgctcac caaggagttt ggcgctgaag ttaatattaa
    12121 aaaggaatcc cagaaggtga gcctcaaatc gcttcgcgat gcaaaagaaa ccttatctat
    12181 agacgaaata gaaaaaaaga gggatgagga tgaaggggat agcgactttt ccaacgatga
    12241 ggtaattatc aaaactaccg gtttcgttca tgagaaaatt gatgatttcg gaatggagaa
    12301 accgaaaatt gatttaaaag ttaaggggaa aattgatttg gataacctat cctcccagcc
    12361 caggagttct aaaaaggaaa cccccgttga gtcaacaaca agcgaagtcc ccgttactcc
    12421 ccttaaacaa gcaaccaaag aggtggccac aaaaaaagag agtaaggaag atgagggtgc
    12481 caagcctgaa aggactaagg ttaaaacacc caaggtggta ggaaaaattg acctggacaa
    12541 aaccagcaaa aagaaggcta aagaaaagcc tgaaaaaaat agtaaccaga aaaccaaaga
    12601 agaatcagct atagttgaaa atgagatgca acccatcgtt gaggagaagt tggaaacagt
    12661 cccttcggtt caagaggtga ttgagaaacc catagaagtt gagagggaag aaatataccg
    12721 atcaacggtt gaaaagttgg ccggtcctac aataaagggg aaaattgata ttaccgcctt
    12781 tgaaaccaag aaaaagcagc ctgttgcatc ttctgatgcg gttaaaaacg ataagaaaaa
    12841 acgccgaaaa cgaataaaga aagatgttgg gagggttcag cttcccgaag cggagcagca
    12901 gggaacccca agaagtaacc ccccattact agtgaagaaa gccgatttgc ctgttaacaa
    12961 taagaagagt aatgctggtg gtagtaagaa gaaacgcccg gtaaaacagg aggttgacga
    13021 ggaagccgtt cagcgtcaaa taaaggaaac ccttgccaaa cttactacca aaggtaaaac
    13081 caagggttca aagtatcgca gggaaaaacg cgaaattctg aacgcccgtc agcaagagga
    13141 aaatcagcgg cttgagctgg agaagaaaat tctgaaagtt accgagttcg tgtcggtcaa
    13201 cgaactagcc tccatgatga gtgtaccggt taccgatgta attgaagtgt gcatgaatct
    13261 aggccttatt gtttccatca accagaggct tgatgccgaa accatgacta tcgttgccga
    13321 tgaatttgga tataaggtgg agtttgtcag cattgaactt caggaatctg taaaagacca
    13381 ggaggatgat tctcccgaaa tgcttaaacc ccgcccgccc atcgttaccg ttatggggca
    13441 tgttgaccat gggaaaacct ccctgctcga ctatatccgt cattcaaatg taattgccgg
    13501 cgaggcaggt ggaataaccc aacatattgg tgcctacgga gttgagctgg agaacggcaa
    13561 aaaaatcacc tttttggaca ctcccggtca tgaggcattt acagccatgc gtgcccgcgg
    13621 tgccaggctc actgatgtgg ccattatagt tgtagctgcc gacgatgccg ttatgcccca
    13681 aaccattgag gctatcaatc atgccagcgc tgctggggtt ccaatagttt ttgccataaa
    13741 taaaattgat aagcccaccg ccgaccccga aaagataaag ggggagctgg ccaaccttaa
    13801 ctacctggtt gaggactggg gcggcaaata ccagtgtcag gaaatttcgg ccaagaaggg
    13861 caccaatgtt gacaagctgt tggagaaagt tctgctcgag gccgaaatgc tcgatctaaa
    13921 agctaaccct gacagaaagg cctcgggaac ggtgattgag tctgcattgg acaagggaaa
    13981 gggatatgta gctaccgtgc tggtggattc aggaaccctg caccatggag acataatcct
    14041 tgccggaata tactacggcc atgttaaagc catgttcaac gaacgaggag ttagaataac
    14101 cgaggccggc ccatcaacac ccgttcaggt ggttggactt aacggtgctc ctcaggcagg
    14161 cgactacttc cacgttatgg agtccgacaa ggaagcaagg gagcttgcca acaagcgcga
    14221 gcagctggtg cgtatgcagg ggctaaagac caagaagcat ataacccttg atgaaattgg
    14281 ccgccgtatt gccattggca acttccagga gctgaacatc gtggtgaagg gcgacgtgga
    14341 cggttcggtc gaggcgctct ccgattccct tatcaaactt tcaacccctc aaattcaggt
    14401 caacgtcatc cacaaggctg taggccaaat ttccgaatcg gatattctgc ttgctgcagc
    14461 atccaacgcc attgtacttg gattccaggt tcgtccttcg atgggggcaa gaaaacttgc
    14521 cgaaaaggaa gaaattgaca ttcgccttta ctccattatc tacgatgcca ttaatgaaat
    14581 cagggatgcc atggagggca tgctgtcgcc cgaaattaag gaggaaatta ccggcaccgc
    14641 cgaggtgctt aatgttttta agatatccaa ggtgggaacc gtggccggct gtatagttcg
    14701 ggaggggaaa attagccgta gcaacaaggt aaggttaatt cgtgatggaa ttgtggttta
    14761 taccggtgat ttagagtctc ttaaacgctt taaggacgat gtcaaagagg tttcggcagg
    14821 ttttgaatgc gggttgaaca tcaacaactt caacgatatt aaggtgggtg atatagtgga
    14881 aggtttccag gaagttgagg tgaaaagaaa gttgtagcaa tactcacgta tttagggaga
    14941 taactatttt ggggtctccc ttttttttat tttgatgttt tgggagtttt atggaagctg
    15001 taaatttttt aagtagtact aaaagaggtg caatagcaca ccgattaacc caatagtgct
    15061 ctgattaacg caggttgaat actttgtgag atagtttgtt ccgttccttc agcgtgccat
    15121 taaaacaatt attattaggc tatgtttaat tgaaaatcat caattacatt tgtttcgtta
    15181 actaatcgct aatgacttta ccaacgggta gctattcaca ggaggagcta agctggtttc
    15241 gggctatttt cgacgactac tatgaaagta tccgtagttt tgcctactat aaaactggga
    15301 gcgttgacct ggctgatgat gtggtgcaag aagttttcct taaactctgg aacatccgtt
    15361 cctccgttcg caacgatacc gtgaaaacat tgctttacac cattgccaca aacctgatta
    15421 aaaaccactt caagcaccag cgggtagtgt ataactttca ggttagtaaa tccactcagg
    15481 acgaggctga atctgcagat gccaacgtaa ggatggacga tatgaaccgt cagcttcaag
    15541 acgctttggc ttccattccc gatgccgcca gggaggtatt cctgatgaac cgcattgacg
    15601 ggtttactta cagtgaaatt gcccagcgcc ttaatttaag cgtgaaaacc gttgagaaaa
    15661 ggatgtcaga agcaatacgt ttgcttagga ataaatttgg ctataagcta tgaccactcc
    15721 aaaagaacat actaagcagg tgagcgaaac agggcatgaa gagttgatgg atgccattaa
    15781 agctttgcag gtacccccgt ccactcggtc gaaagaggac gcatggaata tgattatgca
    15841 gtcaatagct acgactgtgc aacccaagga aaccacaagg attaatagcc tgagggtatg
    15901 gcttacaacc gcagctgcag ctgcgatagt ggttgtttcc tgttttagct acctatggtt
    15961 ctcaagggtt tcggttagct ctcccagagg agtagctacc gatgtgattc tacccgacgg
    16021 ttccacggct aagcttaatg ctgattcatg gttgaagtac ccaaagtttt atagcctggt
    16081 gggtagaaag ctgaaaattg aaggcgaggt tttctttacc gttacaccag gtagaagttt
    16141 tgttgtgagt gatgatttta aaaggcaggt tgaggtggtt ggcacgaagt tcaacatggc
    16201 aaccaggggc tccactttta aggtggcatg tttctcagga agcgtcatgg tgagtaatcc
    16261 gggttcggaa agggtacagc tgggaaaggg cgaggtggcc gaggctgctg gtaaaactct
    16321 gaaggttagc aaggatgagc ttcatatagc tgacagccct ggttggacaa atggagagtt
    16381 taaatttgtg ggtgtcccgc tttcagaagt gcttactgaa cttgaacgcc agttcaacat
    16441 aaaagttacc gcaatggatt ttaatcctaa tggtagaact tttacaggct actttactaa
    16501 atcgagtctt gaacaagccc ttgagctggt agcccaacca atgtcccttc gctacgaggt
    16561 ttcctctgat tcaaccgttg ttaagattta tagcaacaaa tagccgtagg gtttttattc
    16621 attcggttgt attattcaca gggtgaactt tttcgttagg ctaaggttaa atttatggtt
    16681 aaccggtttc gaatagcagg tgctgttgta gtgttttcac ttctggcatt aatggccatt
    16741 ccccatgcgc ttatggggca agcgcttatt accgagaaat tcagtaatgc gcccttggta
    16801 gaatcactca acctgattag caagaagtac gcggtaaaag ttgcctttga caacgaggct
    16861 ttatcgggct ataacataaa cgcaaggttt actaatgagc ctgttgagaa agtgatgtcc
    16921 cgcttgctaa aggacactaa ttttgagtac atcgtaattg acaatgttta cgtcattcgg
    16981 agaaaaatag ccgttaccac cgatactcca aaagtaaatc ccctgaaatt taatggtgtg
    17041 gttcgcgatc gaaacagtgg tgagtcccta ccctacgctt ccataaaggt tgtcaacacc
    17101 cagctgggaa cggccgccaa ttccgatggt tttttctcgc tggcgcaaac acaaaccgat
    17161 tcgctggcta aagctgattc cctgtatatc agggtgagct atgttggcta taagccaata
    17221 gaaatgaaag tagccaggtt tagccacact aattcacccc tggtggtgga actcgatagg
    17281 aatgaccttg aaattaaaga ggtgaaggta accgacgagt actcgtctat agttaaccat
    17341 ggttttcgtc cgggtgaact ggtgtggagt tcgagacagg tagcccaggt gccaaccttt
    17401 tctggaattg atgccattgc acctttaatg cttttacccg gagttgacgg aaccaccgaa
    17461 tccctgtctg gcttaaccgt aaggcattct gctgccgata aaaacctggt aacattcgac
    17521 ggctttacac tctacaacat agaccacttt tttggatcat tctccagcct taacagcaag
    17581 gtagtaaagg atatccgtat tttaagagga ggatttgatg cgcagtgggg tggtagggct
    17641 tcttcggtaa ttgaaataac cggcaaaacg ggaaatgagc ataacctggt ggttgatgca
    17701 ggcgccgatg agttgtctgc tgatgtttcc gtggagggac ctatagggaa aaagttaagc
    17761 tatgtagtag ccgccagacg ttcgtttacc gactactaca ggtcgccgct ctataactcc
    17821 ctgatggggg ttactcgtcc cgatttttct tcagttcagg ctctggccaa cacttctgat
    17881 gcagatgtgg taaagcccac attttactac tatgatgcca acgctaaagt ttcctttaag
    17941 cctaacattc gggacattat ttcggtaagc ggttatacat ctggcgataa gctaacctat
    18001 aaccagaatg tgcaaaatcc atacgttgac gaaaacgccg atttgagcac aacaggtgtt
    18061 gggtttcgtt gggccaggca gtggggaagc tcgttctacc acaaccttac aattggagga
    18121 tcgtcattta acctgaatta cgtgcatcag gatagcatta tgaggtatag gcaggcttcc
    18181 cagcgcgata ccgtattgaa aaactatgac ctggagaatg ttctaaacga tttttcctta
    18241 aacctgctca tgcagtttaa acccgacgtg cataataccc ttgagggcgg gttgagttct
    18301 aacctggtca acctgaacta ctttgaatcg tatttccaca atgtgaatgg gttaagcatc
    18361 atcgatacca cccatagttc tgataacctc tcggcgttat actccggttg gcttcagaac
    18421 acctttgctg gcggcaagct aaaagcttta accataggtt ttagggccac ttaccacaac
    18481 ctctcgggta aattctactt tgaacccaga ctgcaggcca tagttgcacc cacacctaat
    18541 ttaagcctta agttggccgc aggcatttac aatcagtatg taaatagaat tgtgcaagcc
    18601 aactcatcgg gcgtttaccg gaatttctgg atagtttcag atggtaaatt gtttcctgtg
    18661 gtgaaatcgc aacatttgat tgctggtatg acatgggagg acaaaggatg gctggtagat
    18721 gttgaaggat acctgaaata caccgagggc atttctttta tacaaaatgt tacccggcgg
    18781 gtgaatggga accagtttaa aacaacctcc ttgctactta cactcgacag tagggcaacg
    18841 ggagttgatt tgctggtcag gaaggattgg agtaaggggc aggtgtgggc agcttactcg
    18901 ctcagccgtt ccttcaatga atctccaaaa ctgaacggcg gagaggagta ttatgctctg
    18961 gatgatcacc tgcatgaact taaaattgcc ggaacattta ctgtcggcaa gtggcacctg
    19021 tcagccagct ggatttatgg atctccaaaa ccctgggacg aaattcttct aacatccagc
    19081 ctgcagcttt cgcccgacta tgtgaagaat tctgttaggc taatacccta ccataggctg
    19141 gatgccgggc ttgcctttgt tcaacgagtt gacaaagttg aggtaacagc tggtgtaaag
    19201 gctttcaacc tctacgatag gcaaaacgta atagctaaac ccttctccct gtccgatacc
    19261 cccgttcagg attacctggc aggaagggca ttaattacgt ttcaggaaat tattggaatg
    19321 gattttacgc caatgttcta cctgaattta aagttttagc ttcttcaacc tttttactac
    19381 cctgattttt cagctctctg accatcctta gcatcacctt gttgggaacc gctaccagcc
    19441 tgtatcggga gttgtacatc accttgccgc tttgtccaca gctaacgttg gccgcataaa
    19501 actttccggc cttttccttt tcaagctgaa cccagtagtc aacttgacct tcaggttcca
    19561 gaatggttga attttccagc ctaaatatct cgagtgattg gtatagcggc gaaattccct
    19621 ctgggtacac cgacttgttg aacaacatgg ctatttcctt gtcaacatct gttaccggtt
    19681 ttcttatcgt gcttatcagc atggggctgg ccaacgatac agagaaaagg aaacttagta
    19741 gagcggcatt tgaaagccct aatagctttc gaaatatatg cacttgctcc ttgttatagg
    19801 taatgggaac gtgccggccc cgctcctcca tacctattcc atttgaacga atatcggcaa
    19861 acgacctgct gttgtcgagc gtaaaacaat cagcaccggt tctggcaaac agatacctct
    19921 gcgattcgct tatgggcgaa cgctcaaaac caagatcgat agcttcgtta attccaacta
    19981 tgttgccgaa gaaaattcga aatgtattaa aattcaagga ctcattcttt tgtagaaagt
    20041 tgactatcag ggagaaaata tataagcctg gaacaactat ttccttgccg agttctcgca
    20101 tgtgctgctg gttgtgaatg ggattttcgt cggcagttga cttacaaaag ttttcaacct
    20161 gctccccggt tatagtagta gtgaattgca tatatttttt tctcaaaact acctaagttt
    20221 tacaagttaa acaaacgttt taactttttt caactaagga gccaacattg taacgatatt
    20281 gcctttcggg taacatccga gccaggtagt ttatcgtgtt tatccattac tccgatttga
    20341 agatgacagg aaaattccgt tttcaacatt ctccaagttt actaaaaaga accaggctgc
    20401 atttgagcgg aatattttac tggctagtaa aatgactacc cccttgctcg atgatattta
    20461 gcgtctcata atctgaaatc gtactctgaa atgaaaagct tgaggcaata tttatttaaa
    20521 aagtggtagg gtaaaatctt ttctggttgt attaatggca aaagcagatc gggaaatgaa
    20581 acgatttcaa aaaataccgg ataatagctt tgagaggaac aaaacttctc tcgggaatga
    20641 gcttttccag tcgtttcgcg ttccagaggg aatcgggcac gatgcttcat gggagtcaat
    20701 tctaagaagc atcaaatcac ccagcatttc tgtttcacca cgcataccca acctgatatt
    20761 caaggtagca gccactctaa ttgtgttgat tggtttgggt tcgatgattt ggctttacgg
    20821 attgggtaat gttgaggtgt cggttcccta cggccagctg gccacccatg tactacccga
    20881 cagtagccag gtaacgctta atgcagatac caggttaacc tataaccgaa atagatggtt
    20941 gcttcaacgt agggttagac ttgatggaga aggcctgttt aaagtaatga agggaagcag
    21001 gttcaccgtt caaaccgaaa ccgcttctac ctctgtgctt ggtacggttt ttaacgtatt
    21061 tgcccgtaag ggcgttaccc gggttagctg cctggaaggt aaagttaaag tggtggcaaa
    21121 ggaaagcaaa aaggaagcgt atcttactaa tggaatggag gctaaaacta tgggctttga
    21181 gctgagtaaa cctgactcca aaaaggagga attcaaaacc tcatcatgga ctaaaggtga
    21241 attttactac accaataccc caatagggca ggtgtttgac gagcttgaac ggcaatttga
    21301 cgtggacttg tttttcgatg gtgataccac cagaacctac accggatact ttaaaaagga
    21361 caacctgaac agcgctctta ggctggtttg tatcccaatg gatatgaagt ggagcgttaa
    21421 cgatacttta gttataatta atgaaattaa gtagctacta atcataacaa aaaacccaat
    21481 gtttaactta aattttaatg tcatgaaacg attaaatgtg atttttgcag caagcctcat
    21541 gatgatggcc gcaatcagct ttacttcctg tcagaaggat gatggggata caaccgaccc
    21601 acttgtaact accatgcagg aaggcgacga ggtggacagc tacttcgacg aaaccatgtc
    21661 cgaggtggat gaattaacct acgagagtga ctccaaatcg caaatggata tggctgtgat
    21721 taacagcatg ggacaaggaa cccgcaccag gaaaacttcg tgggagggtg atattaggat
    21781 tgacacggtt acctataata actttgtcaa tggcaacgcc aggtttgagc gcgtgaagaa
    21841 tgggcagatt ataattcgca ccacaggcca ttatcttggc gaaaccttta tccgtgaggt
    21901 gtcgttcaac aacttcacca ttaatggcaa cctcatcgaa gggaacaagc gaattcaaaa
    21961 aacttcccag tacacctacg agatacagct tgaaaacggg aaggtaactt tttccgacgg
    22021 gactacccat acaagaacca tgacccgcac cagaactatg gtagaaggct atgatactcc
    22081 ctactacatt tgggatgatg tttacaccat tgagggtagc tcaaccggag ttaacaggca
    22141 gggtaatgca tacacacacc aaataaccaa tgccctggtg gttaggatgg attgccgttg
    22201 gattgtttcg ggtactttca atgtaaccgt taataacaat agcatgtcac tcgactatgg
    22261 aaatggagtt tgcgataacc tggctattgc aaccattaac ggaagggcct acgaggttag
    22321 gctgagaaag taatccaaac aatgtgtaaa cctaaaggga ggcagtagcc tccttttttt
    22381 gttaaaatat atgaataaat atgtaaatat atacatacat gtattaaatt tgcaatgtta
    22441 accattaaaa ttcaaacaaa atgcatgctg tagttgttaa tcaggagaag tgcccaagaa
    22501 atcacccctg cccggtagta aggatatgcc caacgggggc aatcagccag aagagttgga
    22561 actcggcacc tgaaattagc gctgatgatt gcctggcctg tggcaagtgc gtaacgagtt
    22621 gtgggtatgg tgccttcagg tttgataatg cgggtgcgaa atagctactt tacagttcaa
    22681 ctacataaat tgattactca aatgaaaaaa ctatcaggat acttcttgtc agttctactc
    22741 gttgctgcca tgctgagcgc atgcgacggg aacaaccctg caaagggtca gcaggagtca
    22801 gctccagcag cgcctaccaa aacgggttca aaaattaccg gtacctacgc ggttgatatt
    22861 accagggagg aattcctgac taaaattctg gattatactc aggagcctac aaatgtcaaa
    22921 tatcttggca ataagcctgc cattgttgat ttttgggcta gctggtgcgg gccatgccgc
    22981 atagcatccc ccatactcga ggagctcgca aaggagtatc acggtaaaat tctggtttac
    23041 aaggttgata cccagaagga gcagcaaatt tcagccgagc tgggcatcca gagcatcccc
    23101 acattcattt tcttcccagt ggacggcaag ccctttgcta cttctggaat tgccagcacc
    23161 cccgaggata cccgcaaaat gttcgtcgaa attattgaaa aggaactcct aggaaaccgc
    23221 tagccccata agcatagctt aaattgggaa ttacttactc ttggctactt tttacctcag
    23281 cagtcagctg cttgaggtaa attcgctttg ttcggaagtt aatggacgag acgtaacgaa
    23341 ccaaaaagtc gcaccccagc aaacctgcaa tatccattcc tgcaactttt gagtagaggc
    23401 ttcgcagggc gtcaaggtcg gtaaccgcag ccacaaaatc actaaaagta aagcctccta
    23461 ctccgatgta tggaaaaatt gcaagttgga tgctgatttt atccgtattg aaaccaacag
    23521 ccagcgtttc atcaactccc tcaacccgca agccgctatc agaggatatg tctataagcg
    23581 agcgtgatgc tccggtgtca acgatgaggt gcaccgattt accactcatg gaggcttcca
    23641 ccaccatatg atagctgtcg ggctccagct ccactatacg aaaggggatt gtttggcgca
    23701 ttcaatagct tgtatgaaga ataactcctg gttaacttct ctattctgct gcaaagctag
    23761 caatcttttt atcatttggc ttttctcgta atggtctgct tttgctcccc gagattttga
    23821 aatcaaaaat ttagctaatt tctatcaatt ttgaaccaat tactccctat gatgttcttg
    23881 taattacgcc tgaaaaccgt atttttgcct atccaaaaga tatgtttaaa ccacgtaaat
    23941 tttaatataa acattcatat tggttttacg atttaacaat caaatagaca ggcaggttga
    24001 taggtcattt tgcatttaaa aaataagtta agttgaaata ctcacatggc gctttatgat
    24061 tttatagctt gagccgtatg caattgctgg taagcttgtc taggttgaaa aaataaactc
    24121 tttagcgata taccagagca tgcaatatta aaagaatgag ctaaccgctt agtatcaaga
    24181 gtaaaattgc gcaaagagtt ttaaatcttc atgccgaccg gtatctgcct tgatgcattt
    24241 tgtaaagcta agcgataaat tagcgttgga agtataggac tttctagtgg agcaaccata
    24301 gtctctgacg ttgtgcagag taaatgggag ttgtgccaac agggcgggag tattacagag
    24361 taatcaaaac caaataacta ctaatcaagc atataactaa attttattct tatgaaaaat
    24421 tcaagaaaag accttgtgcg aataggagtt ttttacgatg gaaactactt tttgcatgta
    24481 agcaactact acaactactt tcatccaaga aaaaagaggc tgaacattgc cgggttgcac
    24541 gagtttataa ggaatttggt ggcttcggag gaggaagcag atcctcggaa ctgccaggtg
    24601 atagaggccc atttttttag gggcaggata agcgcccagg aggcaagcca acggggtaac
    24661 cagctttata acgaccgggt tttcgacgat attttgatgt ccgagggcgt tgtaacccac
    24721 tacctgccaa ttaggccacg gggtggacgc cgtgaggata agggtataga tgtttggttg
    24781 gcactcgagg cctttgagca aaccttctac aagcgtttta acgttttggt gcttatcacc
    24841 tccgatggcg attatgttcc attaattcgt aaggtaaact ctttgggaac taaggtgatg
    24901 gtgctctctt gggattttga ctacaccgat gaccagggtc gcgaggttac cacccggacc
    24961 tcgcaggagc ttattcgcga ggttacctac ccggtggcca tgcacgagct gataaactcg
    25021 gggttgaaaa gtaaggacag gttaaccgaa aacctcttca ctgttcagga ctttggtcaa
    25081 gctaaaccag tggaaaagcc cgttagcccc gaagagatca aacaagccag agtccttagc
    25141 ctgaatagcg gctacgggtt tatccagtat cctcccaaca accttttctt ctactacctt
    25201 gatgtggtgg acggcgattt cgacgagtta attgaggggg atatggtgga gttcaccatc
    25261 tctaagaacg acaggggcca ggatgtggct aagaatgttc gcaaaattcc tgagtacaac
    25321 gccaataaat ggtagttggt ggcaaattaa aatggctagc gtaacgggtt gggcgagaat
    25381 tcgctcaacc cttttaagct gaaattgttt tcctgagaat tttatggagg gaaaaaacta
    25441 ccgtatgtat gccggaccta aacatccaaa tcaacaacgg taattcctgc tccgccaaac
    25501 tcctcctgtt catcggagaa agccttaaca cccggtgtag atttaaggta atcgcgaatc
    25561 acactgcgga gaatgccgtt acctttccca tgaagtattc ttacccttcc gaagccaagc
    25621 attaaggcat cgtcaactag attttgtact gtttcaagag cctcatcagc tcgtgctcca
    25681 cggatgtcaa tatcggattt gaagttgagg cggcgcttgt aggtatcgaa acccattcca
    25741 acctgaggct gggttacagc cttgttggca ttcctaaact ccgctgttga tatcctttgc
    25801 accctttcta ggtttaccat gctgataagg ttaccaacgg caaccgatac attttgaccc
    25861 gacaggccta ccacttctcc aagtaggtcg cttccgataa tttttaccct atcgccaatt
    25921 gtcaatggag gggatgctac aggttctgtt ctgctgcttg ctgccggttc ttttgctttt
    25981 ctatgcaacg cccttttttc ttgtttttgt ttgatgcgct ccatctttcg ctctacctcg
    26041 tcgttaaggt tatgggcagc ttcgtcaacc tgctgcttaa actgctctag ctcctgacgg
    26101 gctttgcggg tttcctcctt ttgtgcattc gattctttaa taatccttat ggtgttttca
    26161 attttcctat tggtctcagc tagcagctcc tgtgcatttt tctttgcagc agccagaatc
    26221 tccttacgct ggctggctag ctcttcgagc tccctggtca gctgtgcttc ttctgcttcc
    26281 gcttttttgc tggccaaacg aatgttttcc cgttttttct cccagtaccg cttatcgcgt
    26341 gagatttcac gtaaatgttt ttcaaaggta acatagtcat cgcctacctt tgccgtagcc
    26401 tcgtttataa tgctgtccgg taggccaatc tttcgtgcaa tttcaaatgc gtagctgcta
    26461 cccggtttgc caatttccag cttaaatagg ggttctatac gggagctgtc aaaaagcatg
    26521 gcgccattta caattccacg agtgctggcg gcaaaatgct tcaggttgga gtagtgggtg
    26581 gtaattacac cgtaggttcc ggtattggca aagccctgaa ggattgcctc ggctattgct
    26641 cctcctgccg acggctcggt gcccgaccca aactcatcga tgagaatgag tgtttcaggt
    26701 gtggcatttt tcaggaaata cttcatgttt agcaggtggg agctgtaggt gctgaggtcg
    26761 ttctccagcg attgctcatc gccaatatct atgaagattt tgcggtataa gcccatttct
    26821 gaattttcca atacgcaggg cagaaaaccg cactgcagca tgtactggag taggccaacg
    26881 gttttgaggc atactgattt accgcccgca tttgggccgg aaatcagaag tattctctct
    26941 tcggatttaa gttcaatatc cagcgggacg atttcctttc cttcgcgttt caagctcagc
    27001 tgcagtagtg ggtgcttggc ttgtcggagg taggtgccgg tggtctcctt gattatgggc
    27061 tttgcaccct gatactcaat ggcaaatagg gctttagctc taataaaatc aaccattccc
    27121 ataaaatcgt atgctccaag cagctcggga agataggggc gaatactatc ggagaactca
    27181 accagtattc gaacaacctc gcggcgttca tcgtactcca gctcccttat ttcgttgttc
    27241 agctctacca cctcaaccgg ttcaataaaa accgttttgc ctgttgatga ttcgtcgtga
    27301 actattccct taagcttacg cttattagcc gctgcaaccg gaataaccac cctgccctcc
    27361 ctaaccgagg gtgaggcgtc ctgttcaata atgccctggt cgcgagctga tctcagaatg
    27421 gtttgcacca gctttgaaac ggatgattgt tttgatgtta tagccgagcg aatttgggcc
    27481 agctgtggag aagcgttgtc tttaattctt ccaaacttgt cgagtagggc gtttatgcgc
    27541 tccaccacgg tggggtaata ggctacgttt ttggccagct cccagatggc gggatacctg
    27601 tcggtaaatt ttttaaagaa gctaagaagc gcccgaatgg tttccagcga ggtgcgaagc
    27661 tttagtaatt ccgactcatc gagccatgcg ccctgagccg ctattttcct tagcgatggg
    27721 gttaaatcgt agaaatgatc cacagggaag ccttcttcca tcaggcagat ggatttcatc
    27781 tcatcaacct gcgccagcca ggttgatatc aggctgtagt ctgttgaaaa ctgaacagat
    27841 tcaacatgtt gcaaaccaat tgggcagagg cagcgcccag ccaccagctc ccttaccctg
    27901 tcaaatccca ctttttgctc aaagctgtcg gggtaaatgc cctgcattac attattttcc
    27961 ttttatgtag aggtaaaaaa caaggattaa aaaaccaact gtaacctgct gcaaagttac
    28021 aaaaatgttg caggcttaag gtcaatggaa aaagaatggc cggtgggtaa accggccatg
    28081 aagatgcaag gactgcttag tagttgtttg ccttttcctc gaagtatgcc tgaggatgct
    28141 gacacaccgg gcagttcttg ggtggctttt tccccttatg aacgtacccg cacttccggc
    28201 acacccactc aatttcttcg tcgcgttcaa aaactttgtc tttttcaacc ttttcgagaa
    28261 gccttttgta gcgcttttcg tgttccactt ctacctttgc aaccattttg aaggctgtgg
    28321 caattctcgt aaaaccttcc ttttcggcaa tatctgcaaa atgggggtag agctccgacc
    28381 attcctcatg ttcacccatg gctgctgcct taaggttttc ggcggttgtt ccaataacac
    28441 cagcagggta tgaggcggta atctcaacca tgcccccctc aaggaatctg aagaattgct
    28501 tggcgtgctg ctcctcttgt agggctgtag tcataaaaat ttcggctatc tgctcaaagc
    28561 cttcctcctt ggcaaccttt gagaaaaagg tgtaacggtt ttttgcctgc gattctcctg
    28621 caaaggcctt caggagattt ttttcggttt cggttccctt aacgctcttt tccattttgc
    28681 ttgactttta gttttgttat actcccagct aaggtagcaa aaatagttaa cagtctttta
    28741 tgtaaaactt taacttgttg gaaaacccta ccttgatgct acaagacttc caataatatt
    28801 attgattcca taaaacctat ggcttaagta taattgtgtg agttgattat taatttaaaa
    28861 aatatcaaaa aaatacaata aaaaatatcc cgttgggctt ttttttacat taaattgctc
    28921 aaaaacaacc taaccaattt aaacctattg actatgaaaa acattgcact tcaggaaaag
    28981 gtgaacgctt ttatggcaag ccttactgca aaaaaccccg gcgaaacgga atttcatcag
    29041 gccgttaggg aagtcgttga gagcttaatg ccttttatag acgagaatcc gatatactcg
    29101 gacgaaaaga tcttggaacg cattaccgaa cccgaaaggg tgattatttt tcgtgtaccc
    29161 tggatagacg acaggggtaa ccttcagata aaccgaggct acagaataga gatgaacagt
    29221 gccattggcc catacaaggg gggattacgc tttcacccaa cagtcaacct gagtattctt
    29281 aaatttctgg cattcgagca ggtgtttaag aatagcctca cctcactacc catgggagga
    29341 gggaaggggg gatctgactt cgatcccaaa ggaaaaagcg ataaggaggt tatgcgcttt
    29401 tgccaaagct ttatgacgga actttaccgg catattgggc ccaataccga tgttcctgcc
    29461 ggagatatag gagttggggg ccgggagatt ggatacctgt ttgggcagta caaacgtata
    29521 gtaaacgagt ttacgggtgt gcttacaggc aaggggttga agtggggagg gtcgttaatt
    29581 cgccccgaag cgaccggtta tggcaacgtg tattttgcac aggagatgct gaaaactcgc
    29641 ggccaaagca ttgagggtaa aattgttacg gtttcaggtt cgggcaatgt ggctcaattt
    29701 acagttgaga aggcagttga actaggaggg aaagtggtaa ccctttccga ttccgatgga
    29761 ttcatctatg attcagaagg cattaacact gaaaagttgg cgtatgtaaa atggcttaaa
    29821 aacgataaga gggggcgtat caaggagtat gctgacaagt ataaatgcga gtactacccc
    29881 gccgaacgtc catggagtat aaagtgcgac attgcactac cctccgcaac ccagaacgaa
    29941 attaatgggg aagaggcaaa aacgctggtt cgaaatggct gtatgtgtgt ttcggagggt
    30001 gcaaatatgc cttcgacacc cgaggctata gaggttttcc tgaatgccaa aattcttttt
    30061 ggccctggca aagctgccaa tgctggtgga gtagctactt caggactcga aatgtcacaa
    30121 aatgcacttc gcctttcatg gacccgtgaa gaggttgaca accgcctccg ccttatcatg
    30181 tccaacattc acgagacctg tatgaaatac ggtaaagcag atgatggttt tattaactat
    30241 gtgaatggcg ccaatattgg cggatttgtg aaggtagccg attctatgat agcccaaggt
    30301 gctgtgtaga aagtttttca cccatttggt taaaatcgca tcttttggcg atagcgtgcc
    30361 taaacttgat attcaggaga gtggggttaa tctttcttta ccattttgat gcaacttgct
    30421 agcttatgtc atattccttt tcaaaattac cacttacatt tggcagaaaa atgcactcat
    30481 caaattttta aacccgaagt gccaacttgt aattattctt tatttatgga gatagttaag
    30541 ttgagcagat catttagttg atcttcctac ggttgctaat ggttaggaaa acagataaat
    30601 gatgggtggc aggttgcaaa agcaatgtgt taacgggagt aatatgaaac atgaagctta
    30661 atgcgaacat tatgtcccgt taaattcttt taaaatacag taaatagatg atgtttgtag
    30721 agaccgacat taaattttac aagatatgaa tgtaggaatt cctaaagaat tacttcaaca
    30781 tgagctaagg gttgccgcta ctcccaaaac cgtggttaga ttgaaaaagc agggttttga
    30841 ggtgttagta gagtccggat gtggtgtccg ggccaagttc tcggacgagg agtatgtagc
    30901 gacgggtgct gaggttgtct cttcggctca agagttgtac tcatcctcgg atataatact
    30961 aaaggttcaa ccgcctaccg aagccgaggt tggtttgatg aagcgtggca cattgctatt
    31021 aagctacctg tttcccgctc aaaatttgcc cctactgggc aagcttgccg aggcaggtat
    31081 taatgctata gccatggatg ccatccctag aatttcgagg gcacagaaaa tggatgtgct
    31141 atcgtctatg gccaacattt cgggatacag agctgtagtt gaagccgcta actattttgg
    31201 acggttttta aacggccaga ttaccgctgc agggaaggtt gagcctgcca aagtgctggt
    31261 tatcggaact ggagttgcag gcctggcagc tattggcacg gcaaaatcgc ttggagcaat
    31321 tgttagggca ttcgatacgc gtaaggaggc cagagagcaa attcagtcca tgggagcaca
    31381 attcataacg gttgagattg acgaggatgg ctccactgct tcgggctata gcaaggagat
    31441 gagccaggct tatattgatg ccgaaatggc gctgtttaag aggcaagctg ctgaagttga
    31501 tatcatcatc actactgcgc aaataccagg gaaggccgct ccaaagctgc tgcttgagga
    31561 acatgttatg gctatgaagc cgggctcggt tattattgat ctggcggctc ctacaggtgg
    31621 taactgcgtg ctaaccaaaa aggatgaggt ttacacaact ccaaacggtg tggtgattgc
    31681 cggaagggtt gatacccttc ccacccaggc cagcttccta tatggcaaca acctgtgtaa
    31741 cctgctcgac gatatgggaa aagcggaaaa tttcaggata gacatgaacg acgacgtcat
    31801 caagcgggca atggttactt acaatggcaa ggtaaactgg cctcctgagc cgctggcggt
    31861 tagcgcacaa gccaaaaaga ccgaagctgg taagccttcc cctgaggagg aagctgccaa
    31921 aaaggccgga aaagttaaaa aagcgctgat ttccaacgtc atttggcttg ccgttgtcgg
    31981 agccttatta ttcctcctgg gaaaagttgc acctgctgat ttcatggggc atatcaccgc
    32041 ttttgttctg gctgtttttg ttggctggaa tgttatatgg aacgttaccc atgccctgca
    32101 taccccgttg atgtcggtta ccaacgccat tagtggtatc attatcatag gcggtttgct
    32161 gcaaatacag aacgattttt ctgaccctag aaccattatg gcattcatag ccatactgat
    32221 tgccttcatc aacattgttg gcggttttac ggttacctat aggatgttga aaatgtttaa
    32281 aaagtaaacg gagtatgatt tcaaccaata ttcaaatagc agcctacctt ttcgcctcaa
    32341 ttcttttcat tctaagcttg tcggggttat cgtcacagga atcggccaaa aagggtgtaa
    32401 tttatggcat tgtgggcatg gcaattgccc tgtgtgccac cctgttcggc ggtaatattc
    32461 aaggctacgt ttacattatt gttgccatgg tagttgcttc ggttattggc gttattgttg
    32521 cccgcaaggt gcagatgacg gccatgcccc agctgattgc ccttttgcat agttttgttg
    32581 gactagctgc cgttctggtg ggttttagct cattcataga ccccaaaacc gttagcatgg
    32641 ctggcgccga gagggctatc cacatggtgg aaatttttgt gggaatattt atcggggcca
    32701 tcaccttcac cggttcaatt attgcctggg ggaagttaac ggaaaatatt accagtaaac
    32761 ctcttgtatt taaaggaaag gatgtggtta atgtagttct tttgttggct tcagtagtct
    32821 tgggcgttat gtttgttggc cagggtggca ctcatggtat gccgttcctt attgccatga
    32881 ccgttattgc agcatttttt ggagtaatgc tggttatggc aattggaggt gctgacatgc
    32941 cggtagtaat ttctatgctc aactcgcttt cgggatggac cgctgctgct tcgggtttta
    33001 tgctgggcaa cgatttgctg atagttacag gggcgctggt gggcagctcg ggggctatcc
    33061 tttccattat catgtgcagg gccatgaaca ggtcgttttt tgcggttatt tccggaggct
    33121 ttggccagga ggtaaaaacc ggtagaaagc tggagggtaa ggttaactcg atgacgcacg
    33181 atgaggcggc agagtggcta aaagaggctc aatctgttgt aattgttccc ggatacggca
    33241 tggctgtggc aaaggcacaa tatcctatac acgacatggt ggaaaaactt cgaagtcagg
    33301 gcaaatcggt taaatttggc attcacccgg ttgccggaag gcttccgggt cacatgaacg
    33361 ttcttcttgc cgaggccaac gtgccctacg atatcgtgct ggagatggac gaggttaacc
    33421 cggaacttcc cgaaacagat gtagccatgg tggttggagc taacgatacg gtgaaccccg
    33481 gagcgcagga agacccatca agcccgattt acggaatgcc cgtggtggag gtttggaaag
    33541 ccaaaagagt gattgttatg aagcgttcca tggctgttgg gtatgccggg gtggaaaacc
    33601 cacttttcta caaggaaaac gtgagcatgc tttacggcga tgcaaaggat agcgttgata
    33661 aaatcaatgc aaaacttggc tagttcggac cagcaagtaa aaatcccggg aaaccgggat
    33721 tttttgtatc tattttgcag atgtgagaag gataaagaat tcaaaattca aagacctgtc
    33781 cgcctaccgg cggaacttgt caggctcctg acgga
//
LOCUS       DAOSHM010000026        31793 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_1100_length_31793_cov_20.908627, whole genome shotgun
            sequence.
ACCESSION   DAOSHM010000026 DAOSHM010000000
VERSION     DAOSHM010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 31793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31793
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1100_length_31793_cov_20.908627"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(96..566)
                     /locus_tag="PK839_05755"
     CDS             complement(96..566)
                     /locus_tag="PK839_05755"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HOG72276.1"
                     /translation="MRNPYQNQPSHGCFCCSSHNPIGLKLEFWFDDERGTVGTQWLPS
                     ENFQGYHDVLHGGIQSTLMDEVASWCIYILAGTAGVTSKMEVAYKKPVQISNGMVSVV
                     GRMEKMEKRLAIVKTELFDGKGILCSTGQITYFTYSAEVAKQQFGYPGLAEFLS"
     gene            698..1300
                     /locus_tag="PK839_05760"
     CDS             698..1300
                     /locus_tag="PK839_05760"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOG72277.1"
                     /translation="MHGNATKMKLMETARELFSAKGVDRVGVREIAAKAGVNLSLMNY
                     YFESKENLLEQIFEFSVRENGHKLRQILSSGLPLDEKIRQYVYMYIDILIDDPLLVPF
                     VLSTIHRKADKSPYLKSIHTLYSTESFSKQLKEEADKGHIRPIDTEQFFVSMISLIIF
                     PFAVEPLVKYRMSLNNDQMNIFLAARKEHVFDMLMAVLKA"
     gene            complement(1425..2792)
                     /locus_tag="PK839_05765"
     CDS             complement(1425..2792)
                     /locus_tag="PK839_05765"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOG72278.1"
                     /translation="MDYDVVVIGAGPAGYVAAIRAGQVGLKTAIVEKKLIGGMCLNWG
                     CIPSKAIIESGRLYRRIKDDARFFGVDGIDEQNITFSWEQARKRALQVAKQLTSGVEL
                     MLKKNGVEIINGEARITSENSVTVANRNITASNIIIATGSHHLPLKAKLPSEKVVELE
                     QFYSLKELPEHIAVVGEGSIAIELAQFFNLIDRNVTILVPGERMMPLADEYLAGWMLS
                     KLKRDGIRVIFNANDMADSSHFEGNQLVVGKDKFPCDIIINSKLRQGIIPPSDIPLET
                     ANGFIATNDFFQTSFPNIFAVGDVNGEMMFANAASTQGFHTINFIKGIKEPFDKTRYP
                     LNMYSSPEVAQIGKIESQLKADGVNYRVSELPLSANGKALSEGNTEGFVRILSEVKYG
                     QVLGVQIIASNATDMISEAAAFMEAEATIYDVAKTVHAHPTISEVFMEAGFVAVDSAK
                     EENRQ"
     gene            complement(2809..4095)
                     /locus_tag="PK839_05770"
     CDS             complement(2809..4095)
                     /locus_tag="PK839_05770"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HOG72279.1"
                     /translation="MRYIFNFPDIGEGLDEGTIAEWYVQKGQHVEAGDPLVNMETDKV
                     VTDIPSPKSGTIVATYGKIGETIRVGSPLVEIDIEGVEGEAAVAEVRKEEPVEEGAGV
                     VGTLEVAGNSAFLPAGDEGFEQQPAADSKIGRKALATPVARAMAKELGIDINRVTGTG
                     PSGRVTTQDIENFLKKGGTQKSLSVEPSAAMVASGEQLVEYEPLTQIRKTIARNMIQS
                     KQNAAHMSVFDEVEVSELVRIRAKYKDAFAEKGVKLSYLPFILKALAVALSHHKTLNS
                     QLDLENNRIIYKKYFNIGIAVDTEEGLVVPVIRGVDKLSVFEIAERVNEIAEKARNRK
                     LTLDDMKDGTFTVTNYGSIGGQFAVPVINYPQVGILGIGRLVEKPVISHGELAKGMVL
                     PLSLSVDHRIVDGGEVARFLNRLIAYLADPVSLIME"
     gene            complement(4122..5099)
                     /locus_tag="PK839_05775"
     CDS             complement(4122..5099)
                     /locus_tag="PK839_05775"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HOG72280.1"
                     /translation="MKVMTMVQAINNALDIKLQEDPMIVTYGEDVGVEGGVFRATEGL
                     QKKHGTNRVFDSPLAESAIVGTAVGMAANGLKPIVELQFCGFVYPSYNQIISHVARFH
                     NRSRGRYKMPMVIRFPYGGGINALEHHSESMETIYGHIPGVKVVIPSTPYDAKGLLIS
                     AIESEDPIIFMEPKRIYRAIKQEVPEEKYSIPLGKAKVLQQGTDITLIAWGAMVREAQ
                     KAIVMAKKDGIAVELIDLRSIYPIDRQTIVESVRKTGRVVIAHEEPLTFGTGAELTAI
                     INEEAFLSLEAPVNRVAGFDTIVPLPRGEHHYIISPEKIYYNILKTMKF"
     gene            complement(5229..6317)
                     /gene="pdhA"
                     /locus_tag="PK839_05780"
     CDS             complement(5229..6317)
                     /gene="pdhA"
                     /locus_tag="PK839_05780"
                     /inference="COORDINATES: protein motif:HMM:TIGR03181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HOG72281.1"
                     /translation="MLSDFSPLEDRMFQVMDNDGRVINPKYKPDIEPERLVKAYKDML
                     FARQADLMAVSYQRQGRMYTYPPNFGQEAISGGLAVVMREQDWLVPAFRELGAWLAKG
                     ATLKDVFLYFIGYEDGTVFPNAQNILPISVPIASQLQHAAGIGYAINYKKEDSVVYAI
                     VGDGGTSEGDFSEAVNFASVWKAPVVFIVQNNQYAISVPFKMQTRSVNIAVKSYAFGI
                     PGIKVDGNDLLAMTSALQEASAHARAGNGPVLIEALTYRRGSHTTSDDPTKYRSKEEE
                     EAMAATDPIDRLRKYLIGQKLYDASSEGELISQFKQEVERQFGEAENYHPYPLEDVFK
                     YTYVDMPDELKQQKIEHEKFLKWKESLK"
     gene            6574..7545
                     /locus_tag="PK839_05785"
     CDS             6574..7545
                     /locus_tag="PK839_05785"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004351088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOG72282.1"
                     /translation="MKYSVVIPVYNRPLEVKELMESLTEQAFKDFEVLVVEDGSSIPC
                     KQEVLKYKGRLNLTYLEKENTGPGLTRNYGACSANGEYLIFFDSDCLIPNGYFQEVEK
                     ELASAPADFFGGPDRAHPSFTPIQKAISYSMTSFLTTGGIRGGKHKLDKFLPRSFNMG
                     VTAEAFRKVEGFSAMRYGEDLDLSLRLVAAGYSSRLFPGAFVYHKRRTDFRKFYRQVF
                     SSGFARINLHVRHHGSMKLVHLLPSAFVVGICLLLVASIFFPWLLMLPLLYCFALFTD
                     SLYKGNSPWVALLCIPASWVQLFGYGLGFIAACWKLTMRGKNYFLPD"
     gene            7952..8461
                     /locus_tag="PK839_05790"
     CDS             7952..8461
                     /locus_tag="PK839_05790"
                     /inference="COORDINATES: protein motif:HMM:NF038262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SiaB family protein kinase"
                     /protein_id="HOG72283.1"
                     /translation="MHQGNIIIAYKGSVNADIIADTLSLVEVKLNDEMEHSLTRKKVY
                     NVLVECLQNLFHHVDTVPGFYNEDNFGVFILSKIDEGFRVSTGNMVNGQKKEILKQKI
                     DKINSLDKEELKEFYKFVLNNQMFSDKGGGGLGLIDIARKTGNKLEYTFTPFDESFDF
                     FTLSATIND"
     gene            8480..8860
                     /locus_tag="PK839_05795"
     CDS             8480..8860
                     /locus_tag="PK839_05795"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HOG72284.1"
                     /translation="MELLSIEGTPKTPTVTMNPNTGVIEIKGRSIPENSIEFYKTIID
                     WIDEYSKSPQPNTVVNIQFEYFNTSSSKCILDVFKKFEMLKKNKADVVINWYYEEDDE
                     DMLEAGEDYESIIKVPFKMIQIVD"
     gene            9042..10055
                     /gene="murB"
                     /locus_tag="PK839_05800"
     CDS             9042..10055
                     /gene="murB"
                     /locus_tag="PK839_05800"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HOG72285.1"
                     /translation="MPVIKKNVSLKPYNTFGVDVSASYFVKANTLEKILYAINFASYN
                     QLPIFIIGGGSNMLLTKNLNGVMVNPFIQGLTLTEETDSNAIVKVGAGMTWDALVNIA
                     VDHGWGGIENLSNIPGSVGAAPIQNIGAYGVEAKDAIFKVEAIEIKTRKSLEFSNSEC
                     RFGYRDSIFKQELRGKVIVTHVWFKLSKKPEYQLAYGNLKEEVEKLGGVSLKNIRQAV
                     INIRSAKLPDPAVIGNAGSFFKNPVVEDVLFQKIQQDYPDVPHFSVSESHVKIPAGWL
                     IEQCGWKGKRVGSCGVHDKQALVLVNYGGATGEEILTLARSIQQSVYDRFHINLDMEV
                     NIC"
     gene            complement(10094..11875)
                     /locus_tag="PK839_05805"
     CDS             complement(10094..11875)
                     /locus_tag="PK839_05805"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HOG72286.1"
                     /translation="MDTKINERLQLLRRQMKSRGFDAYVIHHSDPHISEYIPDYWKVR
                     EWASGFSGSAGTLVLTEKKAALWTDSRYFLQAEQQLAGTGIELCKQGLPDTPDIPGWI
                     KLNLEAGSTVGCNGGIISVSAARYLSKSLEQLGFKCSFDDDIASTVWLDRPPLPTAKA
                     YIHAAEFAGISAGDKLESIRRNLLNINATACLMCSPDEVCWTLNLRGSDIQFNPVVLC
                     YAYVDLERAILFVDGRKLDDEISNFLTSAGVSTMGYEQVGNFISNISRHTVLVVDSDK
                     TNYKLYNQIPQTVKIVERIGIVTEIKAKKQKPEVEGIHQAMVQDGVALVNFFYWLEEV
                     LGRERLTELTVAKKLLDFRSKQQHFVSESFETIAGYRDHGAIVHYSATPESDYTIEQD
                     GFLLIDSGGQYLNGTTDITRTVHLGKPNADEMKHYTLVLKGMISLAKARFPFGTRGSQ
                     LDTLAKMALWSNHLTYGHGTGHGVGYFLNVHEGPQQIRPENHLPIEPGMVMSDEPGIY
                     ISGCYGIRIENLILCTDDISNDFGRFLTFETLTLCPIDTKPIAVSMLSDDERDWLNHY
                     HATVYEKLSPYLDEEHKTWLKKKTLPL"
     gene            complement(11919..14750)
                     /locus_tag="PK839_05810"
     CDS             complement(11919..14750)
                     /locus_tag="PK839_05810"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOG72287.1"
                     /translation="MNGNSLSSKESETHIYLQHIHRYLRAGFWLWNSETNHLLLGHKF
                     QEALGVKADELGIHLDDFFSVVHPDDRRQLSTILFRLLKQKLRYREFDVRVMVNDVWH
                     RLRIHALVGEIATDGSVQSLFGKFLPADPRQPISGPAIPASTFMGFDAFTYNPEEGQL
                     QWQFQPSSMFWGNTPKGKTWNFSEFRAILSPSSFKELSQSWQVFANGNEEFFTRNLTL
                     SSPQNAKRIITLLARKVEGSRYIQGVMIDTSKLCGIADKSSFLHFYSSIASSHRLLMI
                     ELSREGAIKGCNRITEKSFGYSLAELQNPQLLPSLFTNADSFKQLVTYANSGGSKPFV
                     CRVRNREGQVKDISWILLKLPEVVGMEGNTLIGQDVTRQVQVNFEVNLLQQRYQAIKL
                     ANAELQGAANFEGVFSCFGQQLERIFPSMVTVVLSYNAADGFITVEGIFGIQHKTWES
                     LIFDLGWNPVGRRFLLHDKDKHAIKSAAAYRVNESFTGIMEGYISMSASKIIERYINI
                     EAIYAEGLVSGNHMYGGVLLLKPEPADEPDLTLLEELASVAVNTIQRIDTAEGLQQQI
                     FTLKSKIEQKDELFSQLSHQLRTPLNAVLGFSQLLLELNQPDDLLKKYIGIINSKGKQ
                     LLRLVNDTIDYNLIEKGNLTLVKSTFNLNHLLQQLHRSFSQQFELYQNEGIKLKFIQP
                     GNAENIFLKTDEGRLEQALANLLDGIFKTVERGTIELSCRKENSKVMIFLRISGAGAE
                     SKVNESLLDQFRQSENIESPMQSLLGIRMARQIVTLLGGEMRFDIGSNDNAEVEVLLP
                     LTDSSTSGSNNNIEDNAPVYPDFKNKVILIVEDEEVNYLLFRELLVSCGATTLWAKNG
                     REAVNLVSTINQTIHLVVMDIQLPVMDGYAATMEIKQINPKIPVIAETAYISADDRKK
                     AEAAGCDGYITKPIEPKVFYEVLEIFLGT"
     gene            complement(14882..17383)
                     /locus_tag="PK839_05815"
     CDS             complement(14882..17383)
                     /locus_tag="PK839_05815"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOG72288.1"
                     /translation="MPEKQDKTKVTAAFYFFKAGLLLLLMVTVMVVKGQTGQLLVVGF
                     VTDSVGSPVPFASVVVEGGPIGVSTDINGRFRITLPAAQSAVLTISCVGYKSTSVLIN
                     ADVNSPIVELRVKLDVNELLIDEIRVSSDRRWTGNIERVNARDISFMPNVSGNFESII
                     KLMPGVASTNELSSQYSVRGGNFDENLVYVNDIEIYRPFLVRSGQQEGLSFINSDLVS
                     AVEFSAGAFNAEFGDRMSSVLNVSYRMPTSYQSGVSLSLLGASAYTEGVLGNRKFSYI
                     TGVRYKTSSYLLNTLDVKGDYTPSFLDWQGLFSYRLNDKLAFSFLGNYATNSYQFAPD
                     VRETRFGVFSNALQLKIYYEGKELDRYISGMGAAYVEYAPSQNTSIKIYSANYLTHES
                     ETFDLLGEYFLNELDNSMGSGSYGDSLINVGIGGFLNHARNFLFARINNFGLVGSVRF
                     SNAKLKWGAQLQHEFFDDELNEWNLLDSSGYAIPYNGSAIGLSNVMVAGNSTSVNKLS
                     AFSQLDYSLSRGAFTLATNTGLRLLYWDYTGDVLVSPRVSLSLKNQRLKNFNFHLAGG
                     VYYQPPFYRELRMPNGSLNPNIKAQRSVQLLAGLEYYFTAWNRPFKLSVELYNKWLDN
                     LIPYKVDNVRIRYAGQNLAKGFARGVDIKLNGELLEDAESWVSISLLKTMEDILHDSY
                     VDANGQTVYPGYYPRPTDQTLGFNLFFQDYLPGNPTFRVHLSANYGTGLPVGVNGIDR
                     YDVTFRMKDYKRVDIGFTKILRDETSSNGISRKLKGFKGIWLGAEVFNLFNFNNTVSY
                     LWIQTVGNQQGESDTFAVPNYLTSRRLNIRLQLKF"
     gene            17592..17999
                     /locus_tag="PK839_05820"
     CDS             17592..17999
                     /locus_tag="PK839_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002559836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOG72289.1"
                     /translation="MIQQVEFALPKFPIGFHLITELVVKKLPALPETGMANIFIKHTS
                     AALTINENADYTVRKDFQKFISTLVPFSNTYEHTLEGEDDMTAHIKSSLLGQSVWIPI
                     SNGKLNLGTWQGIYLCEFRNHGGNRKLVITIIS"
     gene            18298..19908
                     /locus_tag="PK839_05825"
     CDS             18298..19908
                     /locus_tag="PK839_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008780255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOG72290.1"
                     /translation="MEKLLLLGDEAIAQGAIDGGLSGIYAYPGTPSTEIMEYVQGSKM
                     AAERNIHRQWSANEKTAMEAAIGMSYAGKRAMVCFKHVGLNVAADAFINSAITGVNGG
                     LVVVSADDPSMHSSQNEQDSRFYGKFAMIPILEPSNQQEAYDMAYQSFELSERLNVPV
                     LFRITTRMAHSRADISRHEVKDQNKLRMPLDPRQYVLLPAIARKNYKKLLSTQPAIEL
                     EAEQSKFNAFIPGENHDMGIVATGIAYNYLVENYPNHQCPFPVVRIGHYPIPRKKITE
                     LYESCKSLLVIEEGYPMVEEMLRGFFNVGKPIHGRLDGTLPRDGELNPNIVAKALGFP
                     AAGEEPLAEVVKNRPPSLCMGCGHRDVYTDLNEAIKDYSAGRVFSDIGCYTLGALPPF
                     NAINSCVDMGASITMAKGAADAGLVPAVAVIGDSTFTHSGMTGLLDAVNENSPITIII
                     SDNSTAGMTGGQDSSATGKIVDICRGMGVDPSHIRVVVPLKKNHEENVKIIREELAYN
                     GVSVIIPQRECIQTFARRKKTEKKESND"
     gene            20018..20599
                     /locus_tag="PK839_05830"
     CDS             20018..20599
                     /locus_tag="PK839_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009275907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HOG72291.1"
                     /translation="MNNDIILAGVGGQGILSIAAAIGLAAVNSNLHIKQSEVHGMSQR
                     GGDVQSHLRISDAPIASDLIPAGKAHLIISVEPMEGLRYMPFLSKNGWLVTNITPFDN
                     IPDYPKVDEILKRVKAVKNHIALDADSIAKECGSTKASNIVMLGAASPFIDIPAAGIE
                     EGIRQLFNRKGEDIVNLNLKAFRAGREYSEKNR"
     gene            20855..23494
                     /locus_tag="PK839_05835"
     CDS             20855..23494
                     /locus_tag="PK839_05835"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HOG72292.1"
                     /translation="MYKLLPPFCIAILLFAVQPAQAFLDEDKKICQAVRVNPKPPVVD
                     GSLDDEVWEKGEWSGNFIQHEPYNGNEPSQKSYFKILYDSDYLYAAFKMSDSEPDKVV
                     SRITRRDDADGDRVTLCIDSYFDQRTAFLFTTNAAGVKSDEVMTENGDKEDMNWDPIW
                     WAKAVKTPDGWTAEMKIPLNQLRFANSSDMKWGVQVMRYIYRNQETDLWQHVPKDAPG
                     FVHLFGELHGLNGLAPKRQVEVAPYMVTKTERLEAESGNPFMTGKNNGFNAGVDAKIG
                     ITNNFTLDLTVNPDFGQVEADPAVVNLSAFETYFEEKRPFFIEGSGMMNFSLMFGDGD
                     LATQNLFYSRRIGRAPQLQPDLDDNEYAKIPSNTAILGAAKVTGRTGKGMSVGILETI
                     TNSTYATIDSMGVRTHEIAEPLTNYTVASVRQEFSGGNTIISGIATSTNRQLNDLSSD
                     YFHQNAFTGGMDLQRFWSNKKYLFCGKLYFSHVNGSKEAIARTQTSPARYFQRPDNNY
                     ARLDSTRTSLTGTGGLLQFGRVGEGHLQYMGAVTWHSPQLEINDIGYIQNVDDIFQVM
                     WVGLRFWKPTLWFRYVNFNLNQWATWNFGGQSTDKGGNINFNLQFTNYWTFNAGINYN
                     SESLSAHALRGGPMMKIPGSWNAWYNIGTDGRKKLVFYMGGSTLESNENYAYYQSVYA
                     TLSYRPLKNLQLSLNPSYSVNSDKLEFVENVDYGNETRYIQGKLSQKTFALSLRVEFS
                     INPDLSVQYYGRPYLSSGKYTKFKRITNPMANSFDQRFVTFEPSQLVYNQDEEEYYVD
                     ENNDGGSDYSFSNRNFNFMDLQSNLIVRWEYRPGSTLFLVWTLGKQAYESEYGSSLNN
                     NIETLYDAHPHNIFLLKFSYRFN"
     gene            23815..24363
                     /locus_tag="PK839_05840"
     CDS             23815..24363
                     /locus_tag="PK839_05840"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013618053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HOG72293.1"
                     /translation="MKIYTKTGDKGMTSLIGGMRVPKYHPRIDTYGTVDELIAFIGLL
                     RDQPVDGHTRDMLVFVQDKLMSCAAVLAAEEPNSKTDLPTVSDADVRKLEADIDAMDA
                     LLEPLTSFVLPGGHPTVSLCHVCRTICRRAERLAVKLATETPLPVNLTIFLNRLSDYL
                     FTLSRKLAKDLKVDQIKWHPDK"
     gene            24449..24670
                     /locus_tag="PK839_05845"
     CDS             24449..24670
                     /locus_tag="PK839_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004362633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HOG72294.1"
                     /translation="MYWTLELASKLEDAPWPATKEELIDYAIRSGAPLEVIENLQELE
                     EETEIYESIEDIWPDYPTKEDFFFNEDEY"
     gene            complement(24891..27992)
                     /locus_tag="PK839_05850"
     CDS             complement(24891..27992)
                     /locus_tag="PK839_05850"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOG72295.1"
                     /translation="MSESILKALMQLFAIISRPVAEEEMEANDYSDRRRVVEEFLRNQ
                     LNQELVAEYIKVFDNYYNLYQEKQSEKTKSKKRTSSSSVRVLKICAQINEELQQQQKI
                     VVLVRLLEFVKPEEGKVTEQEMAFISTVAETFYVSADEFNMLSDFVLSSFNQIPITPN
                     VLLIDGNKDFELPGVKHIYAEGLLDELRVVFIESANIFFLRYRGTHELYLNGHLIQNE
                     KIFVLSNGSSIRNPKIKPVYFSDIVGRFALDKLKERIVFEALDVEYRFRSGKIGIHQM
                     TFTEESGHLVGIMGASGAGKSTLLNVLNGSEKPTAGNVLINGIDIHRQPELVEGQIGF
                     VSQDDLLIEELTVYQNLYFNARLCFDNYTPQQLDEAVSRTLVNLGLNEIRDMRVGTPL
                     NKKISGGQRKRLNIALELIREPAILFLDEPTSGLSSRDSENILDLLKELTLKGKLVFV
                     VIHQPSSEIFKMLDKLLILDTGGYLIYNGDPIESIMYFKSRVQHANWNESECATCGNV
                     NPEQIFNIVEANVLDEYGNPTRARKISPVEWNNHYKKWFSAQPQLDGIPDFLPKIHFK
                     TPGWFKQLKIFVKRDVLSKLANSQYMIINVLETPLLAFLLSYIIKYFNIDVSNRVGYT
                     FMENSNIPVYLFMSVIVGVFVGLTVSAEEIIKDRKIQKRESFLNLSWSSYLMSKVAIL
                     LVLSAVQAFMFVIVGNSILEVRGMYFEYWLVLFSAWFSSNMLGLVISDSFKTVVTIYI
                     LIPFLVIPQIILSGVIVKYEKLNPKISSPSSIPFYGELIVARWAYEALAVEQFCNNPY
                     EKPFYVFDEAMSISDYKRNYWLKSLQNKVDFCSRNLGLPEKSEEVEDALTLLRNEIMH
                     EMTSPQGSKLTFSKYNQVSMAGVSPELLDELKSYLELVRKYYVKLYNKASSQKDAYIS
                     SFQAAPGGQEAFVNLKRQYYNESLTDFVRNTGEVERITEYKGRLIQKIDPIFLYPDTK
                     FVKAHFYSPVKPVFGKFAGTFWVNVAIIWISNILLYLILYYRLLKRFLEQFDRIGAEK
                     EAN"
     gene            complement(28015..28431)
                     /locus_tag="PK839_05855"
     CDS             complement(28015..28431)
                     /locus_tag="PK839_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72296.1"
                     /translation="MAKKILIPFILIGLFSVKATAQVENLRNTCALNFPPEYVSDGQE
                     YFASLKPDQVIEFRTTFFGENTYRITTCTSVKKGQVVLTVYDTDKNLLFSNRDYDYSP
                     YWNFRFTSTVICIIQVDVVSQQFTPGYVMLLIGFKQ"
     gene            complement(28444..29343)
                     /locus_tag="PK839_05860"
     CDS             complement(28444..29343)
                     /locus_tag="PK839_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72297.1"
                     /translation="MLNFKRIVFFVSGCFLIFGLTECNHPNKSDNQTIKEDVSIDTLP
                     TLIKYESSIFPIPSPWQVVGLVKNLSIPYNENLLNRYDRYQKYGKSFKQALNLGIYGA
                     DISYLCIYDRTQEAIKYLDAIRKLCDQLGLEQIFDQNFFDAVEQNINNKDSLARIITK
                     AYSSSDLYLKTNERSDIGALIVAGSWIESMYFLTQIALGNSNRDIINRFGEQKHPLDN
                     LIELLTPYYYKSSDFSELIDELINVAYEYDGIIYSYNYKEPIIDAANRQITINSTTQV
                     VVSEYHIRIISSKIESIRNKLVE"
     gene            29596..31731
                     /locus_tag="PK839_05865"
     CDS             29596..31731
                     /locus_tag="PK839_05865"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HOG72298.1"
                     /translation="MKKVTLLTAAFMLSLTSVAQQKVLTLEQAVLRQYADFRPQTLAN
                     FSWKANVNAYTYTEGDSIIEVSVPSGMPKTVMTLELLNRLLSADSIPALKRLAYTWAS
                     ENVIKVFSAPYLAFVDVGLGKITRRFTIPEEAENLELSPNNTSLAYTVANNLYITDAK
                     GTPVAVTTNDNPGIVNGQAVHRNEFGINKGIFWSPSSSYLAYYRMDETMVTDYPLVDI
                     TQRIARVEPVKYPMAGMKSHEVTLRVYNLSTHSNIEIKTGLPAEQYLTCVTWSPDEKS
                     IFIATLNREQNHMRLNRYSAQTGELMATLFEETDSEYVEPQQPLAFLDDNSGRFIWQS
                     RRDGWNHLYLYDVNGKLLRQLTRGEWEVTNINAIDLPNGKIYITATRESPIERQVYAV
                     DLKKDNITMLTQGAGFHNALFSPDCKYFVDFYSSISIPNRVNLCTGKGKIVKNLVNAP
                     NPLASYNVSLPELLTLKANDGTTLYGRLIRPVNFDSTLRYPVVVYVYGGPHSQLVRNS
                     WMGGASLWECYMASRGYILFTLDNRGTSYRGSNFEQTIHRRLGTIEIADQLAGVNFLK
                     SLPYVDSTRIGVHGWSFGGFMTISLMLRAPEFFKVGVAGGPVTDWKFYEVMYGERYMD
                     TPQENPDGYSTSDLKQYVKNLKGKLLIIHDDMDKTVVPQNSLTLLHEFVKSNVQVDFF
                     LYPQHEHNVMGKDRVHLIDKIIRYFDDNL"
BASE COUNT         8858 a         7488 c         6913 g         8534 t
ORIGIN      
        1 tgacggaagg ttgagtggtt ctgacggtta tattgttcaa tttctattta tcaactttca
       61 acctctaccc tgtttcctgt atcccttccc tacacctagc ttagaaattc agccagtcca
      121 ggataaccaa attgctgctt agctacttcg gctgaatagg taaagtatgt tatttgaccg
      181 gttgagcaaa gaatgccttt accatcgaat agctctgttt taactattgc caaacgtttc
      241 tccatttttt ccatcctacc cacaacgcta accatgccat tcgagatttg aactggtttt
      301 ttataggcaa cctccatctt tgaagttact cctgcagtcc cggctagtat gtatatgcac
      361 cagctggcaa cctcgtccat cagggttgac tggattccgc catgaagcac atcatggtat
      421 ccctgaaaat tctcagaagg aagccactgt gtccctacag ttccccgttc atcgtcaaac
      481 caaaattcca acttcaaccc aatggggttg tgggacgagc agcagaagca tccatgacta
      541 ggctggttct ggtatggatt tctcaacttt ataaccatgt ttatcgagta gtattattgc
      601 acacaatttt agcattattt cacaaaactt taagcctagt agctctttgt tttttagcga
      661 aagagttaaa tttggtaaac ctataaatct ggactctatg catgggaatg ccacaaaaat
      721 gaagctgatg gaaaccgccc gcgagctatt tagcgcaaag ggcgtagata gagtcggagt
      781 tcgtgaaatt gcagctaaag caggggttaa cctttcactc atgaactact atttcgagag
      841 caaggagaac ctgctagaac aaattttcga attttcggtt agggaaaatg ggcataagct
      901 taggcagatt ctgagctctg gccttccgct ggatgagaaa ataaggcaat acgtttacat
      961 gtatattgac attttaattg atgaccctct tctggttccc tttgttctgt caaccatcca
     1021 cagaaaggca gataaatcgc cctacctaaa atccattcac accctataca gcaccgaaag
     1081 tttctccaag cagctaaagg aagaggcaga taagggacac atcagaccta ttgacaccga
     1141 acaattcttt gtgagcatga tctccctaat tattttccct tttgcagttg agcccttggt
     1201 gaagtatagg atgagcctta acaacgatca aatgaatatc tttctggctg cccgcaagga
     1261 gcatgttttc gacatgctga tggcagtgtt aaaagcatag gttgatgaga gccggcgcta
     1321 gggtaatcgt gcaaaacaaa gtggaagcat ggggcaatat agctgaaaag gccacggctt
     1381 acctagtata ggctccgtga cctcagcagt tcatcctgaa aaggttactg tctgttttcc
     1441 tccttagcgc tatcaaccgc tacaaatcct gcttccataa aaacctccga aatggtggga
     1501 tgggcatgaa cggtcttggc cacgtcgtag atggtagcct cggcctccat gaatgcagca
     1561 gcttccgata tcatatcagt tgcgttgctt gctataattt gcactcctaa aacctgccca
     1621 tattttactt cggataggat tcttacaaac ccttccgtat tgccctccga aagggctttc
     1681 ccattagcag aaagtggaag ttcgctcacc ctgtagttca ccccatcggc ttttagctgg
     1741 gattcaatct tgccaatctg cgctacttcg ggtgaagaat acatgttaag cgggtacctg
     1801 gtcttatcaa aaggctcctt tattcctttg ataaagttaa tagtgtggaa gccttgagtt
     1861 gacgctgcat tggcaaacat catttcacca ttcacatcgc ctaccgcaaa aatgttggga
     1921 aagctggttt gaaagaagtc attggtagca atgaaaccat ttgccgtttc aaggggaatg
     1981 tccgagggcg gaataatgcc ctgccgcagc ttagagttga ttataatatc acaggggaat
     2041 ttatctttcc caacaaccag ctggttccct tcaaaatggc tactatcggc catatcgttg
     2101 gcattaaaaa ttaccctgat tccatccctt ttcagcttgc tgagcatcca gccggcaagg
     2161 tactcatctg caagcggcat catacgttcg ccgggaacga gaatggtaac gttgcggtca
     2221 atgaggttga agaattgagc cagctcaatg gcaatggatc cttccccaac cacagctata
     2281 tgctccggga gctccttaag actataaaat tgctccagct caaccacctt ttctgatggg
     2341 agcttggcct ttaaaggcag atggtgcgag ccagttgcaa taataatgtt gcttgctgta
     2401 atgtttctat tcgcaacggt aacagaattt tcggaagtaa tccgagcctc cccgttgatg
     2461 atttcaaccc cattcttttt cagcattaat tcaaccccgg aggtgagctg tttggcgact
     2521 tgcagggctc ttttgcgcgc ttgttcccag ctgaaagtga tgttttgctc gtcaatacca
     2581 tcaacgccaa aaaatcgggc atcgtcttta attcgcctgt acagcctgcc gctttcgatg
     2641 attgccttag agggaatgca gccccagtta aggcacattc ctccaattag cttcttttct
     2701 acaatggcgg tttttaaacc cacctgtccg gcacgtatgg cagccacata tcctgccggg
     2761 ccagctccaa taactactac atcgtaatcc atatcaatat tttaaagttt actccatgat
     2821 tagcgaaacg ggatcggcaa gatacgcaat aagcctgttg aggaatcgcg ccacctctcc
     2881 accatccaca attctgtggt caaccgaaag tgaaagtggg agtaccattc ccttggcgag
     2941 ctcgccatga cttataaccg gcttctccac cagccttcca atacccagta ttcctacctg
     3001 cgggtagtta atgacgggaa cggcaaattg ccctccaatg gaaccatagt tggtaactgt
     3061 aaaagttcca tccttcatat cgtcgagggt tagcttacgg ttacgggctt tctcggcaat
     3121 ttcgtttaca cgctcggcaa tttcaaaaac gctgagctta tcaacacctc tgattacagg
     3181 aacaaccagt ccttcttcgg tatcaaccgc tataccaatg ttgaagtact tcttgtagat
     3241 tatccggttg ttctccaaat cgagctggga gtttaacgtt ttgtggtggc taagcgccac
     3301 ggccagggct ttcaggataa atggcaggta gctaagctta acaccctttt cggcaaatgc
     3361 atccttatac tttgcccgaa tgcgaaccaa ctcgctgact tcgacctcgt cgaacacgct
     3421 catgtgggcg gcattctgct tcgactgaat catgttccgg gcaatggttt tccttatctg
     3481 ggtgagcggt tcatattcaa caagttgctc cccactggct accattgcgg ctgaaggctc
     3541 aacagaaagg cttttttgag tacctccctt tttaagaaag ttttcgatat cctgtgtggt
     3601 aaccctacct gaaggaccag tcccggtaac cctattgata tctataccca actcctttgc
     3661 cattgcccga gcaactggcg ttgctagagc cttgcgccca attttactat ctgcagcagg
     3721 ttgttgttca aaaccttcgt ctcctgcagg caaaaaggcg ctatttccgg caacctcaag
     3781 ggtaccaaca acccctgcac cctcctcaac aggttcttct tttcggacct cagcaactgc
     3841 tgcttctccc tcaacgccct caatatcaat ctcaacaagc ggggagccca cacgtatagt
     3901 ttctccaatc ttaccgtagg tggcaacaat ggttcccgat ttgggagaag gaatatcggt
     3961 taccacctta tcggtttcca tgttaacaag cgggtcgccg gcttcaacat gttgtccctt
     4021 ctgcacatac cactcggcaa ttgttccttc gtcaaggcct tcgccaatat ccggaaaatt
     4081 aaatatatac ctcatgttta gtagtttaaa ctttcaagca tttaaaattt catcgttttt
     4141 aggatattgt agtatatctt ttcgggtgaa atgatatagt gatgttcgcc acgaggcagg
     4201 ggcactatgg tgtcaaaacc tgcaacacgg ttaaccggag cttcaaggct taggaaggcc
     4261 tcttcgttaa taatggcggt aagctctgcg cctgttccaa aggtaagcgg ctcctcatgg
     4321 gctattacca ccctaccggt cttccggacg gattccacga tggtttgcct gtcaatcgga
     4381 taaatggacc ggaggtcgat gagctccact gcaattccat cctttttagc cataacaatt
     4441 gctttttgag cctcgcgtac catggctccc caggcaatca aagtgatatc tgttccctgc
     4501 tgaagaactt tggccttccc caatggaatg ctgtattttt cctcgggaac ctcctgctta
     4561 atggcccggt aaattcgctt gggttccatg aagataatgg gatcttcact ttcgatagcc
     4621 gaaattagaa gcccctttgc atcgtaagga gttgagggaa taaccacttt tactcccgga
     4681 atatggccgt aaatggtctc catgctttcg gagtgatgct ccagcgcatt tattccacca
     4741 ccgtaaggga agcgaatcac catgggcatt ttgtagcgac cacgtgatcg gttgtgaaac
     4801 ctggccacgt ggctaataat ctggttatag gaagggtaaa caaacccaca aaactggagt
     4861 tccacaatag gtttaagccc gttagcagcc atccccacag cagtgcctac aattgccgat
     4921 tcggcaagag gtgaatcaaa aaccctgtta gtcccatgtt tcttctgtaa cccctcggtt
     4981 gccctaaata caccaccctc aacacctaca tcttctccgt aggtaactat catagggtct
     5041 tcttgtaact ttatatccaa ggcattattt attgcctgaa ccattgtcat tactttcatc
     5101 tgcttaatcc gtataagtga ttgttaatct atttattcca aatcttattg taatcaaaag
     5161 gtggttgccc gaatgatata cccctatcac aacaggctat aactctaatt cgttgccatt
     5221 gggatatttc attttaaact ctccttccat ttaaggaatt tttcatgctc aatcttctgc
     5281 tgcttcagct catcaggcat atcaacatag gtgtatttaa acacatcctc aagcgggtat
     5341 ggatggtagt tttcggcctc gccaaactgc cgttcaacct cttgcttaaa ttgggatatc
     5401 agctcaccct cggaagaagc atcgtaaagc ttttgcccaa taagatactt tcgaagccgg
     5461 tcaatgggat cggtagcagc catggcctcc tcctcctcct tggaacggta tttagttggg
     5521 tcatcggagg tggtgtggga gccgcggcga taggtaagcg cctcaataag tacgggtccg
     5581 ttaccggctc tggcatgcgc cgaggcttcc tgcagcgcac tagtcatggc aagtaggtca
     5641 ttcccatcaa cctttatacc agggatacca aaagcatatg atttaaccgc aatgttgacc
     5701 gaccgggttt gcattttaaa cggaacggag atggcatact ggttgttttg cacgataaaa
     5761 actaccggtg ctttccatac cgaggcaaaa ttgacagctt cggagaaatc gccctcggaa
     5821 gtgcctccat ccccaacaat ggcataaact acgctatcct ccttcttgta gttgatggca
     5881 tagccaatac ctgcggcatg ttgaagctgt gaggcaatgg gcacagaaat gggaagaata
     5941 ttttgtgcat tcggaaatac cgttccatcc tcataaccaa tgaagtagag aaaaacatct
     6001 tttagggttg cccccttggc gagccaggcg ccaagctcgc ggaatgccgg aaccagccaa
     6061 tcctgttccc tcatcacaac ggccaggcca cccgatatgg cctcctgccc gaaattaggt
     6121 ggataggtgt acatgcgtcc ctggcgctgg tacgaaacgg ccatcaaatc ggcctgacga
     6181 gcaaaaagca tgtccttata tgcttttacc aaccgctcgg gttcaatgtc gggtttgtac
     6241 ttggggttaa taaccctacc gtcgttgtcc attacctgga acatcctatc ttctaatggt
     6301 gaaaaatctg aaagcatact attgatgaat ttatttgttt ttacgtaagt gttggtgttc
     6361 taatcgtttt cattaacaac catttaattg aaaatgttca ttaaaacatg ttgaggtgtg
     6421 ctgatttggc atgaatagca ttaagttttc ccaaggatta caaaacacca gcttgttcca
     6481 cagtgattaa aattttataa tcgtccaatc tagctggtgg aagcgggcaa aatattttgc
     6541 attaccttta cctgtctaat aaaaactgct tccatgaagt actccgtggt tattcctgtt
     6601 tacaataggc cgctagaagt caaggagctt atggaaagcc ttaccgaaca ggctttcaaa
     6661 gatttcgagg tgctggtggt agaggatggt tcgtcaattc cttgtaagca ggaggttctt
     6721 aaatacaaag gccgtttgaa ccttacatat cttgaaaagg aaaatacggg accaggctta
     6781 acacgaaact acggcgcctg ttcagcaaat ggtgaatatc tgatattttt cgactccgac
     6841 tgcctcattc ctaatggata ctttcaggag gtagagaagg agctagcctc ggctcctgct
     6901 gactttttcg gaggccccga ccgtgcacat ccttcattca cccccataca gaaagccatt
     6961 agctactcca tgacctcttt tcttaccaca ggcggaatac gtggaggtaa gcataaactc
     7021 gacaagtttt tgccccgtag ctttaacatg ggtgttaccg cagaagcctt tagaaaagtt
     7081 gaaggatttt ccgccatgcg ctatggcgag gaccttgact taagcctgcg gttggtagca
     7141 gcaggataca gctcccgtct tttccccggg gcttttgttt accacaagcg ccgtaccgat
     7201 tttcgaaaat tctaccggca ggtttttagc tcgggctttg ccaggataaa ccttcatgtt
     7261 cgacatcacg gctcaatgaa gctggtgcat ttgctccctt ccgcttttgt tgtaggcatt
     7321 tgcctgttgc tggtcgcttc tatatttttt ccttggctgc tgatgctgcc tctactctac
     7381 tgctttgccc tttttactga ctctctatat aaaggcaaca gcccttgggt tgctttgctg
     7441 tgcataccgg cgtcttgggt gcagctgttc gggtacggcc tagggtttat agctgcatgc
     7501 tggaagctaa ccatgagggg taaaaattat tttttgcctg actaagtagc aaatttgagt
     7561 gtcgtttaaa taaggtcatt ggtggttgac attataccac ctctcaggaa gattagcttt
     7621 tcactcattc tagtaacata atggacatcc actaatacta acacaaagta cctttaaaat
     7681 caggagtaac aaccttacgc atagctattt tagtaaagtc ttctttcact tatcagcccc
     7741 agtagctgaa aaaacttccc aaacccatca ggtaaggttg taacctgaag tagctatccc
     7801 aaaaagcaaa ataataggat gcaaaaaatt aagcgcctac ctcttgcaac tttcaaaaaa
     7861 aagacagtat atttgtttag agataccaac agctggcaaa ttgctcgaaa accaatccat
     7921 ttccgatttt ttaaaccggt gttatgctag tatgcatcag ggcaatatca ttattgcata
     7981 caagggctcg gttaatgccg atattattgc ggatacgctt agcctggttg aggtaaaact
     8041 taacgacgaa atggagcata gccttacacg aaaaaaagtt tacaacgtgt tagtagagtg
     8101 ccttcagaac ttgtttcacc acgttgacac agtgcccggg ttttacaatg aggataattt
     8161 tggagtattt attctttcaa aaattgatga gggtttcagg gtttcaacag gcaatatggt
     8221 aaacgggcaa aaaaaagaaa ttcttaagca aaaaattgac aagattaatt ctctggacaa
     8281 ggaagaactc aaagaatttt ataaatttgt tctcaacaat caaatgttct ctgacaaggg
     8341 aggaggggga ttagggctga tagatattgc ccgtaaaaca ggaaacaagc tggagtacac
     8401 cttcacacct tttgatgagt cgtttgattt ttttaccctg agtgcaacaa ttaacgacta
     8461 acgaactaaa acgaacacca tggaattgct aagcatcgaa ggaactccta aaacaccaac
     8521 cgttaccatg aaccccaaca caggggttat tgaaataaaa gggagatcta ttcccgagaa
     8581 ttcaatagaa ttctacaaga ctatcattga ttggattgat gagtactcaa agtcccccca
     8641 acccaacacg gtggttaaca ttcagtttga gtacttcaac acctcatcat ccaagtgcat
     8701 tctcgatgta tttaagaagt ttgaaatgct gaagaagaac aaggcagatg tggtaatcaa
     8761 ctggtactac gaggaggatg acgaggacat gcttgaagca ggtgaggatt acgaatccat
     8821 catcaaggtt ccctttaaaa tgattcagat tgttgattaa aacgacagag tatcacaatt
     8881 ataaagggcg tttatcgccc ttttttactt catgaactac cacgggctaa gtacaaattt
     8941 atgagcttta cggacaatcc tattaaatat caaccaatcc ctacattcca tgttaccaat
     9001 tattgattac ttttgttgtt gatgatgaaa ataccacaag catgccggtt attaaaaaaa
     9061 atgtttcgct aaaaccctat aatacatttg gtgttgatgt ttcagcatcc tactttgtaa
     9121 aggccaacac gctggagaaa atcctttacg caataaattt tgcatcgtac aaccagcttc
     9181 caatctttat catcggaggg ggtagcaaca tgctgctcac caaaaaccta aacggcgtaa
     9241 tggttaatcc ttttattcaa ggtttaaccc ttaccgagga aaccgactcc aatgcaatcg
     9301 taaaggttgg tgccggaatg acctgggatg cattggtaaa catagctgtc gatcacggat
     9361 ggggtggaat cgaaaaccta tcaaatattc ccggaagcgt aggtgctgca cccatccaaa
     9421 atattggtgc ctacggggta gaggccaagg atgctatttt caaggtcgag gcaattgaga
     9481 taaaaacacg taaatcactg gaattttcaa atagcgaatg ccgttttggc taccgcgata
     9541 gcatattcaa acaggagctc aggggcaagg ttattgttac ccacgtttgg tttaaactat
     9601 caaaaaaacc tgaataccag ctagcttacg ggaatcttaa ggaggaggtt gaaaagcttg
     9661 gtggcgtttc gttaaaaaat atccgccagg cagttataaa cattagaagc gccaagctcc
     9721 ccgatccggc tgttatcgga aatgccggca gctttttcaa aaatcccgtt gttgaggatg
     9781 tgctgtttca aaaaattcaa caggactacc ccgatgtacc ccatttcagc gtgtcggaat
     9841 cccatgtaaa aattccagcc ggatggctta ttgagcaatg cggttggaag gggaaacggg
     9901 taggcagctg cggcgtgcac gacaagcaag cgctggtgct ggttaactat gggggtgcaa
     9961 caggtgagga aatattaacc ctagcacgct caattcagca atctgtttac gacaggttcc
    10021 acatcaacct cgacatggag gttaatattt gctagccacg ttagaataga caaataagct
    10081 gatgttgaat agcttacaaa ggtaaagttt tcttcttcaa ccaagttttg tgttcctcat
    10141 caagataggg cgacaatttt tcgtaaaccg ttgcatggta gtgattcagc cagtcccgtt
    10201 catcgtcgct aagcatactt accgcaattg gcttggtatc aatggggcaa agggttaacg
    10261 tttcgaaagt taaaaaacgg ccgaagtcgt tggaaatatc gtcggtgcaa agtataaggt
    10321 tttcgattcg aatgccatag cagccagaaa tatatattcc aggttcatcg gacatcacca
    10381 tgccgggctc aatgggaaga tggttttcgg gacgaatctg ctgggggccc tcgtgaacgt
    10441 tcagaaagta cccaactcca tgacctgttc catgcccata ggtaagatgg tttgaccaaa
    10501 gggccatttt ggccagcgta tcgagctgcg aaccacgtgt tccgaagggg aacctggcct
    10561 tggcaaggct aatcatccct tttaaaacca gcgtgtaatg cttcatctca tcggcattag
    10621 gcttacccag gtgaacagtt cgggtaatat cagtagtccc gttcaggtat tgccctcccg
    10681 agtcaataag cagaaatccg tcctgttcta tggtatagtc agactcggga gttgcgctat
    10741 agtgaactat tgccccatga tccctataac cagcaatggt ttcaaaactt tcgcttacaa
    10801 aatgctgttg tttgctcctg aaatcaagca actttttggc taccgtaagc tctgttaacc
    10861 tctccctccc caaaacctcc tccaaccagt agaaaaaatt aaccagggca accccatcct
    10921 gcaccattgc ctggtgaatg ccttcaacct ctggtttctg cttttttgcc ttaatttcag
    10981 ttacaattcc aattctctcg acaatcttta ccgtttgggg tatctggttg tacaacttgt
    11041 agttagtttt atcggaatca accaccaaca ccgtgtgcct gctgatgtta gagataaaat
    11101 ttccaacctg ctcatagccc attgtagaaa cgcctgcact ggtaaggaaa ttagaaattt
    11161 catcgtcgag ttttctcccg tcaacaaaaa gaatagcccg ttcaaggtca acataggcat
    11221 agcaaagcac taccggattg aactggatgt cgcttccacg gaggttgaga gtccagcaaa
    11281 cctcatcggg ggaacacatt agacaggccg tagcattaat gttaagcaag ttacgacgga
    11341 tactttcaag cttatcacca gcggagattc cggcaaattc agctgcatga atataggctt
    11401 tagcagtagg aagtggtggc ctgtctaacc atactgtgct tgcaatatcg tcatcaaaac
    11461 tacatttaaa gccaagctgt tcaaggcttt tcgaaagata tcgtgcagca gaaaccgaaa
    11521 taatgccacc attacaccca actgttgacc cagcttctag attcaacttt atccagcctg
    11581 gaatatccgg tgtatcggga agcccctgct tacaaagttc aatgcctgta cctgcaagct
    11641 gctgctcagc ttgaaggaag tagcgcgagt cggtccaaag tgctgctttt ttttcggtta
    11701 gcaccagagt tccagccgat ccggaaaatc cggaagccca ttccctaacc ttccagtaat
    11761 cgggaatgta ctcgctgata tgggggtcgc tatgatgaat aacataggca tcaaaacccc
    11821 tgcttttcat ctgccggcgc agcagctgca atctttcatt tattttagta tccattcaat
    11881 ctatttatta acaaatcaac atcatgtgtt atgtaaaatc aggtacccag aaaaatttcc
    11941 aaaacttcgt agaatacttt aggttcaatt ggcttagtaa tatacccatc gcatccggcg
    12001 gcctcggcct tttttcgatc gtcagctgaa atgtatgcgg tttcagcaat gactgggatt
    12061 ttaggattga tctgctttat ctccatggtt gcagcatagc catccattac cggaagttga
    12121 atatccatta caaccaggtg tatggtttga ttgatagtgc taaccaggtt aacagcttcc
    12181 ctgccgtttt ttgcccacag ggttgttgcc ccacacgaaa ccagcaactc cctaaacagc
    12241 aggtagttta cctcctcgtc ctcaacaatc aggataacct tgtttttaaa atcgggataa
    12301 acgggagcat tgtcctcgat gttgttatta ctacccgaag tactgctatc cgttaggggt
    12361 aatagaactt caacctccgc attgtcgttg gagccgatat cgaaccgcat ctccccacca
    12421 agcaatgtaa caatctgcct agccatccta atgcccagta gagattgcat tggcgactct
    12481 atgttttcgg actgtctgaa ctgatcgagt aagctttcgt taacctttga ttcggcacca
    12541 gctccgctta ttcttaaaaa gatcatcacc ttgctgtttt cttttctgca gctcagctca
    12601 attgtgcccc gttcaacagt tttaaagata ccgtccaaaa ggttggctaa tgcctgttcc
    12661 aaccttccct catcggtttt aagaaagatg ttttcggcat ttcctggttg tataaatttt
    12721 agctttatcc cctcattctg gtaaagttcg aactgctgtg aaaaagagcg gtggagctgt
    12781 tgaagcaggt ggttaagatt aaaagttgat tttaccagag tcaggttgcc cttttcaatg
    12841 aggttataat caatggtgtc gttaaccagc cggagaagtt gcttcccctt gctgttgatt
    12901 atcccgatgt attttttaag caaatcatcg ggttgattca actccagcag cagctggctg
    12961 aaacccaaaa ctgcattcag gggggttctt agctgatggc ttagctggct gaatagctcg
    13021 tctttctgct caatttttga tttaagtgta aaaatttgtt gctgtaaacc ctcggctgta
    13081 tcaatccgtt gaatggtatt aacagcaaca gaagccagct cttcgagtaa ggttaaatcg
    13141 ggttcatcgg caggttctgg tttaagtagg aggactcccc cgtacatgtg gttgccagaa
    13201 accaaaccct ctgcatatat cgcctcaata ttgatgtatc gttcaataat ttttgatgcc
    13261 gacatggaaa tatatccttc cataataccg gtaaacgatt cgttaaccct gtaggctgca
    13321 gccgacttaa tggcatgttt atccttatcg tgcagtagga accggcggcc aacagggttc
    13381 catcccaagt cgaaaattaa cgattcccag gtcttgtgct gaatgccaaa aatgccttca
    13441 accgtaataa agccatcggc agcattatac gacaatacca ctgttaccat gctggggaat
    13501 atccgctcaa gctgttgccc aaagcacgaa aaaacaccct cgaaattagc agcaccctgc
    13561 aactcggcat tagccaactt aatagcctgg tacctttgtt gaagtaggtt aacttcaaaa
    13621 tttacctgaa cctgcctggt tacatcctgc ccaattaggg tattgccttc cataccaacc
    13681 acttccggaa gtttaagcag aatccacgaa atatctttaa cctgtccttc gcggtttcta
    13741 actctacaca caaaaggttt agaacctcct gaatttgcat aggtaaccag ctgtttaaaa
    13801 gaatcggcgt ttgtgaaaag agacggtaat agctgtgggt tctggagttc ggccaggctg
    13861 tagccaaaag atttttcggt aattcggttg catcctttaa tagcgccctc cctgctaagc
    13921 tcaatcatca gcaaacgatg tgacgaagca attgatgagt aaaaatgcag aaaacttgat
    13981 ttatcagcaa ttccacaaag tttggatgta tcaatcatca ccccctgaat atacctgctt
    14041 ccttcaacct tcctggcaag cagggtaatg attcgtttgg cgttctgagg acttgaaagg
    14101 gttaagttcc gtgtaaaaaa ttcttcattt ccgttggcaa aaacctgcca gctttgcgag
    14161 agctccttga agctggaggg tgaaagaatt gccctaaatt ctgaaaaatt ccaggtttta
    14221 ccttttggtg tatttcccca aaacatagaa ctaggttgaa attgccattg cagctggcct
    14281 tcctccgggt tataggtaaa agcatcaaag cccataaatg ttgatgcagg tatggctggc
    14341 ccggatatgg gttgcctggg atcagcaggt aaaaattttc caaacaggct ttgcacactc
    14401 ccgtcggtag ctatttctcc tactaaagca tgaattctca acctatgcca tacgtcgttc
    14461 accattaccc taacatcaaa ttccctgtag cgtaactttt gcttcagcaa tcggaaaagg
    14521 atagtagaaa gttgccttct atcgtcgggg tgaactacgc taaagaagtc gtcaagatgg
    14581 atgcccagct catcagcctt aactccaagt gcctcttgga atttatgccc tagcagcaag
    14641 tggttggttt ctgaattcca cagccagaat ccagccctta ggtaacgatg gatatgctgt
    14701 aggtagatat gtgtttcaga ctctttgctt gataagctat ttccattcat ttttacagag
    14761 gaaaatcctt taacagtatg ctacaatgat gaaccaattt agctttttac tttacaattt
    14821 ataaaaatag caaagttagg ctaaactctc aacatttttt taggaaaaca aaagctattg
    14881 gttagaattt aagctgcaac ctgatattta agcgccttga ggttaggtag ttgggaacag
    14941 caaaggtatc tgattcaccc tgctggttgc caacggtttg aatccagagg taggaaaccg
    15001 tgttgttgaa attaaacagg ttgaaaacct cggcgcccaa ccatatcccc ttaaatcctt
    15061 tcaacttccg ggatatacca ttagaagagg tttcgtcacg gagtatcttg gtaaaaccga
    15121 tgtcaaccct tttgtaatcc ttcattctga aggtaacatc atacctgtcg ataccgttca
    15181 caccaacagg caagcctgtc ccgtagttag cgctaaggtg taccctgaaa gtcggattac
    15241 cgggaaggta atcctggaag aacaagttaa accccagggt ttgatcggtt ggtcggggat
    15301 agtaccctgg gtaaaccgtt tgtccgttgg cgtcaacata gctatcgtga agaatatcct
    15361 ccatggtttt aagtagcgaa atgctaaccc acgactcggc atcctcaagc agctcaccat
    15421 taagcttaat atcaacgccc cgggcaaaac ccttagccag gttttgtcca gcataacgaa
    15481 ttcgcacgtt gtcaacttta taggggataa ggttgtcgag ccatttgttg taaagttcta
    15541 ccgacagctt aaaaggtcgg ttccaggcag taaagtagta ttccaaacca gcaagcagct
    15601 gcaccgaacg ttgagctttg atatttggat ttaagcttcc gttgggcatt cgcagctccc
    15661 ggtaaaacgg aggttggtag taaacgccac ccgctaaatg gaagttaaaa tttttaagtc
    15721 tttgattttt aagcgataac gagactcggg gacttaccag cacatcaccg gtataatccc
    15781 agtagagaag acgtagtccg gtatttgtgg caagggtaaa tgcccctctg cttaagctgt
    15841 aatcgagctg tgaaaaagcc gataacttat tcaccgaggt tgagttacct gctaccatca
    15901 catttgaaag gccaatggct gaaccattat agggaatggc atagcctgag gagtcaagca
    15961 gattccactc gttcagttca tcgtcgaaga actcatgctg aagctgggcg ccccatttta
    16021 gcttggcatt tgaaaacctc acacttccaa caagcccaaa attattgatg cgggcaaaga
    16081 ggaagttgcg ggcatgattc agaaatccac cgattcctac gttaatcaga ctgtcgccat
    16141 aggaacccga tcccattgag ttgtcaagct cattcaggaa gtattctcca agcaggtcga
    16201 atgtttcgga ctcgtgagta aggtagtttg cagagtaaat ctttatgctg gtgttttggc
    16261 ttggggcata ctccacataa gcggctccca ttcccgaaat gtacctgtcc agctccttac
    16321 cctcgtagta tattttcagc tgaagcgcat tgctgaacac gccaaacctg gtttctctaa
    16381 catctggagc aaactggtag ctgttggtgg cataattgcc cagaaagcta aacgcaagct
    16441 tatcattcaa cctatacgaa aacaacccct gccaatcgag gaatgaaggg gtgtagtcgc
    16501 cctttacgtc aagcgtgttc agcaggtagc ttgaagtttt gtagcgcact ccggttatgt
    16561 acgaaaactt ccggttaccc aaaacaccct cggtgtatgc agaggcacca agcagactta
    16621 gagacacacc agactggtag gaggtcggca ttcggtagct cacattcaga accgacgaca
    16681 tcctgtcgcc aaactcggcg ttgaatgctc cggcagaaaa ctctacagcc gagaccaaat
    16741 cggaatttat gaagcttaac ccctcctgct gccccgatct tactaaaaat ggcctgtaaa
    16801 tttcaatatc gttaacgtaa accaagttct catcaaaatt tcccccccta accgagtact
    16861 gcgagctaag ctcgttggtt gatgccacgc ccggcatcag cttgataatg gattcaaaat
    16921 ttccagaaac attgggcata aagctaatat ccctggcgtt aaccctttcg atgttacccg
    16981 tccagcgacg gtcggacgat acacgaatct catcaatcag caactcgtta acatctagct
    17041 tcaccctaag ctcaacaatt gggctattta cgtctgcatt aataagaacc gaagtgcttt
    17101 tgtagcctac acacgaaatg gtgagaacag cactttgcgc agccggaagg gtaatgcgaa
    17161 accgtccatt gatatcggtc gaaaccccta ttggcccacc ctctacaaca acgctggcaa
    17221 aaggaacagg gctgccaacc gagtcggtta caaaccctac aaccaggagc tgacccgtct
    17281 ggccttttac aaccataacg gttaccatta acaacaggag caaccctgct ttaaagaaat
    17341 agaaagcagc ggtaacctta gtcttatcct gtttttcggg cattacaagt tgttgagttt
    17401 atctgtacat attccctcaa aagtagcatg ctgaacaata ccaagcctaa gaataaccat
    17461 atttgcttaa aaaaattgtg ctacaatagc tgtcaacatg gtttacaaat caaatttcct
    17521 tacctttagt tgatttttaa atttactccc gctttcgatt tacctaattg tgaaacataa
    17581 aatgcctgag catgattcaa caggttgaat ttgccctccc caaatttcca attggttttc
    17641 acctgataac cgagttggtt gttaaaaaat tgccggcgtt acccgaaacc ggaatggcca
    17701 acatattcat taagcacacc tcggcagcgc taacaattaa tgagaatgcc gattacaccg
    17761 tacgaaaaga ttttcagaaa tttatatcca cccttgtccc tttttccaac acctacgagc
    17821 ataccctgga aggtgaagac gatatgactg cccatattaa atcgtctctc ctaggccaat
    17881 cggtttggat tcccatctct aacggaaagc tgaaccttgg cacctggcag ggaatttacc
    17941 tatgcgagtt tcgaaatcat gggggaaacc gtaagctggt tattactatc atttcctgac
    18001 gtaacattac ctaccttaaa ttttaacttt tcatcaaaaa agggctttta gcttaatcat
    18061 tctaaaataa ttcctacttt tgctaacaat cagaacccaa ttttgaactg aatggtttaa
    18121 aactgaaatg attttaccat gtattcggcg tattacttat ataacgttgc ccagaacgta
    18181 ggttacctga agcgttttgg tggcctttag aacttgttta gcttatacca ttaccttaat
    18241 agctaaccac gccaaagcaa aagccaaata acaattttga agaataaacc aaacgaaatg
    18301 gaaaagttac tacttctagg cgatgaagcc attgcccagg gagcaataga cggagggcta
    18361 tcgggcattt acgcctaccc tggcacccca tcaacagaaa tcatggagta tgttcagggt
    18421 tcaaaaatgg ctgccgaacg gaacattcac aggcaatggt cggccaacga aaagacagcc
    18481 atggaagctg ctatcggcat gtcctatgct ggcaagcgtg caatggtgtg ttttaagcac
    18541 gtagggctca acgtggctgc cgacgccttt attaattctg ccataacagg cgttaatgga
    18601 gggctggtgg tagtatcggc cgacgatccc tccatgcact catcgcaaaa cgagcaggac
    18661 tcaaggttct atggcaaatt tgccatgatt cccattcttg agccatcaaa ccagcaggag
    18721 gcctacgaca tggcctacca aagttttgaa ctctccgaaa ggcttaacgt ccccgtgctg
    18781 ttcaggatta caaccaggat ggcacactcc cgcgccgata ttagcaggca tgaagttaag
    18841 gatcaaaaca agcttagaat gccgctcgac ccaagacagt acgttttact tcccgcaata
    18901 gcacgtaaaa actataaaaa gcttctttcc acccaacctg ctattgagct ggaggctgaa
    18961 caatctaaat ttaacgcctt cattccagga gaaaaccacg acatgggtat tgtagctacc
    19021 ggaattgcct acaactacct ggttgaaaac taccccaacc accagtgtcc ctttccggtt
    19081 gtcaggattg gacactaccc tattccccgc aaaaaaatta ccgagctcta cgaaagctgc
    19141 aaatcgctgc tggttattga agagggatac cccatggtgg aagagatgct taggggattt
    19201 ttcaatgttg gcaagccaat tcatggacgg ctcgacggga ccctgccccg cgacggcgag
    19261 ttaaatccaa acatagttgc caaggcatta ggtttcccgg ctgctggtga agagcctttg
    19321 gcagaagttg taaaaaaccg ccctccatcg ctctgcatgg gatgtggaca ccgtgatgtt
    19381 tacacggatt tgaacgaggc aattaaggac tacagcgccg gccgggtttt ttccgacatt
    19441 gggtgctaca ccctgggagc tttgccacca ttcaacgcca taaattcatg cgttgacatg
    19501 ggagcctcca ttacaatggc caaaggggca gctgatgccg gacttgttcc agctgttgct
    19561 gtcattggcg actccacctt cacccacagc ggtatgacag ggctactcga tgctgtaaac
    19621 gaaaactccc ctataaccat catcatctcc gataattcaa ctgccggtat gaccggaggc
    19681 caggattctt cggcaacagg taaaattgtt gacatttgca ggggtatggg cgttgacccc
    19741 agccacatca gggtggttgt accgttaaag aaaaaccacg aggaaaacgt caagataatt
    19801 cgcgaggagc tagcctataa tggcgtttcc gtaattatcc cccagcgtga gtgcattcag
    19861 actttcgccc gtaggaaaaa aacagaaaag aaagaatcaa atgattagca atgagctgga
    19921 gaaagtttac catgcatctt tttcggatga acgtagctca gcaattaatc aatatttcta
    19981 acaacgtatt atatatcaac tagttaaaca atttataatg aataacgata taattttagc
    20041 aggtgttggc ggacagggaa ttctttcaat tgctgcggca attggcctgg ccgctgttaa
    20101 cagcaacctg catatcaagc aatcggaggt tcacggtatg agtcaacgcg ggggtgatgt
    20161 gcaatcgcac cttcgcattt ccgatgcacc cattgcctcc gacttaattc ccgccggaaa
    20221 agcccacctg attatctcgg ttgaacccat ggaaggctta cgctacatgc catttctgtc
    20281 gaaaaacgga tggctggtta ccaacataac cccctttgac aacatacccg actacccaaa
    20341 ggtggacgag atactaaaga gggtgaaagc cgttaaaaac catattgccc tcgatgccga
    20401 ctccattgcc aaggagtgcg ggtcaacaaa ggcttcaaac atcgtcatgc taggggcagc
    20461 ctcacctttt attgatattc ctgccgctgg catagaggag ggaatacggc agctatttaa
    20521 ccgtaaggga gaggatatcg tcaaccttaa ccttaaagcc ttcagggctg gcagagaata
    20581 cagcgaaaag aaccgctaat cctctattac cctgcaagag tatacgttgt ttaacccact
    20641 ggaaatgagg gttacaggtt ttctcagaaa ataggtcggc tacattacac attatcgaaa
    20701 aactccagcg acggctggag tttttttttt acgcattggt aaaggtaagc aggtcattat
    20761 ttttgcgtcc tacagcaaat tttcatcccg cttgcagcaa aactcacaac atgtcgtcgt
    20821 tatgttgttg tattgaaaac cttaaaacat aagtatgtat aagttgctgc ccccattctg
    20881 cattgcaata ctgctatttg ctgttcaacc tgcccaggca ttccttgatg aggataagaa
    20941 aatttgccag gccgtccggg taaatccaaa accccctgtt gttgacggaa gccttgacga
    21001 tgaagtatgg gaaaagggtg aatggagcgg aaattttatc caacatgagc cctacaacgg
    21061 gaatgagccc agccagaaat cctacttcaa aattctatac gatagcgact acctgtatgc
    21121 tgcctttaag atgagcgata gcgagcccga taaggttgta tcgaggataa cccggcgcga
    21181 tgatgccgac ggggataggg ttacactctg catcgactcc tactttgacc aacgtactgc
    21241 attcctcttt acaaccaacg ctgcaggagt gaaaagcgat gaggtgatga ccgaaaatgg
    21301 tgataaggaa gatatgaact gggatcccat ctggtgggcc aaagcggtta aaactcccga
    21361 tggctggaca gccgaaatga aaataccgtt aaaccagctt cgtttcgcta actcatccga
    21421 catgaaatgg ggcgttcagg tgatgcggta catttaccgc aaccaggaaa ctgacctctg
    21481 gcagcatgtt ccaaaggatg cacctggatt tgttcacctt ttcggcgagc tgcacgggct
    21541 aaacggactt gcccccaagc ggcaggtgga ggtagcaccc tacatggtta ccaaaaccga
    21601 acgacttgag gctgaatctg gaaatccctt tatgaccggt aaaaataatg gtttcaatgc
    21661 tggcgttgac gcaaaaatag gaataaccaa taacttcaca ctcgacctaa ccgttaaccc
    21721 cgattttggg caggttgaag ccgaccctgc cgtggtaaat ctttcggctt tcgaaaccta
    21781 ctttgaggag aaaaggccat tttttattga gggaagcggc atgatgaact tttcgttgat
    21841 gtttggcgat ggcgacctgg caacccagaa cctgttttac tcccggagaa ttggtcgcgc
    21901 tccccagctt caacccgatt tggacgataa cgagtatgct aaaattcctt caaacactgc
    21961 aatcctcggc gcagccaagg ttaccggacg aaccggaaaa ggcatgtcgg ttggtattct
    22021 tgaaactatt accaacagca cctacgccac catcgacagt atgggtgttc gcacacatga
    22081 aatagcggaa cccctcacca actacacagt tgccagcgtg cggcaggagt ttagcggagg
    22141 aaacaccatc atttcgggca tagctacttc aaccaaccgc cagctgaacg atctctccag
    22201 cgactacttt catcaaaatg cctttaccgg cggcatggat ttacagcgat tttggtcgaa
    22261 taagaaatac ctgttttgtg gaaaactcta ttttagccat gttaacggaa gcaaggaggc
    22321 tatagccaga actcaaactt ctccagctcg ctactttcaa aggccagaca acaactatgc
    22381 caggctcgac tctactcgca cgtcgcttac cggtactggg ggattgctac agtttggaag
    22441 ggttggcgaa ggacacctgc agtacatggg agcggttacc tggcattccc cccaactcga
    22501 aattaacgat ataggataca tccagaacgt tgatgatatc tttcaggtga tgtgggttgg
    22561 acttaggttc tggaaaccta ccctgtggtt tcgctatgta aactttaacc ttaaccagtg
    22621 ggctacatgg aactttggag ggcagtcaac cgataaaggt ggaaatatta acttcaacct
    22681 acagttcacc aactactgga catttaatgc cgggattaac tataacagcg agtctctgag
    22741 cgcccatgcc cttcgtggtg gtccaatgat gaagatacca ggtagctgga atgcatggta
    22801 taatataggg actgatggga gaaaaaagct ggttttctac atgggtggat caacgctgga
    22861 aagcaatgag aactacgctt actaccaatc ggtttacgca accctttcat accgccccct
    22921 taaaaatctg caactttctc taaacccgtc atactccgta aacagtgata agctcgaatt
    22981 tgttgaaaat gttgattacg gcaacgaaac ccgatatatc cagggaaaac ttagccagaa
    23041 aacgtttgcc ctttcgttga gagtggaatt tagcattaac cccgacctgt cggtgcagta
    23101 ctatggccga ccctaccttt caagtggcaa gtacaccaaa tttaagcgta ttaccaatcc
    23161 aatggccaac agtttcgatc aacgattcgt aacctttgag cccagccagc ttgtttacaa
    23221 ccaggatgag gaggaatact atgttgacga gaacaacgat ggcggttccg actactcatt
    23281 ctccaaccgc aacttcaact ttatggacct acagtcgaac ctgattgtgc gctgggagta
    23341 cagaccgggg tcaacccttt tcctggtttg gacgctgggt aagcaagcct acgaatcgga
    23401 gtacggcagc tcgttgaaca acaacatcga aaccctttac gacgctcacc cccataatat
    23461 attcctcctg aaattttcgt acagattcaa ctagccctaa acactcggca ttcgaatgta
    23521 ggcaggaagc acctgcaacg ctccacctgt aggtgttaat cccaacccgc attattccca
    23581 tcagcaactt gaggaatcat ctgaacacat gtcagcaatt gcctaccgca atacttcata
    23641 gaagttcaca gatccggcct tcaaaaaacc tgcagcatca cacccgtgaa aaaattcaaa
    23701 accgacattt aaaaatttat aaaaatttaa tttcaaaata ctaacaaatc ttaaccaaaa
    23761 catgtatgtt tagggtgaag tttgcactac gattatttca cagctagcca taccatgaaa
    23821 atatatacca aaacgggcga caaggggatg acctccctga taggcggaat gcgtgttccc
    23881 aagtaccacc ccaggataga tacctacgga acagttgatg agctaattgc ctttatcggg
    23941 ttgctgcgcg atcaaccagt tgatggccac accagggata tgctggtatt tgtccaggat
    24001 aaattgatga gctgtgccgc agtgcttgca gccgaagaac ccaactcaaa aactgactta
    24061 ccaacagttt ccgatgccga cgttaggaag ttggaagcag acattgatgc catggatgcc
    24121 cttcttgaac ccctgacatc gttcgttctt cccggtggac atcctacagt ttcgctttgc
    24181 catgtttgca gaacaatttg ccgtagggct gaacgactgg ccgttaaact tgcaaccgaa
    24241 acgcccctac ccgtcaacct gactatattt ctgaatcggc ttagcgatta ccttttcacg
    24301 ctttcccgga agctggcaaa agatttaaag gtagatcaga taaaatggca ccctgataag
    24361 taattccaat tttttatata tttgcgggta tatggttatt tataatctat taattatcag
    24421 tttattaact aaaatataaa ttctacctat gtactggaca cttgaattag catcaaagtt
    24481 ggaagatgcg ccctggccgg caacaaagga ggaactcatt gactatgcaa ttcgctcagg
    24541 cgcaccactt gaggtgattg aaaacctgca agaacttgag gaggaaactg aaatatatga
    24601 aagcatagag gatatttggc ccgattatcc caccaaggag gatttctttt ttaacgagga
    24661 tgaatactaa tcattctaaa tggtaacaaa gcaaccagca agcaaccatt caaaagaaat
    24721 tagaaaaagc cacttcggaa tccatgtagg tgaactctct gcccccgcca tgaagggtta
    24781 tttgccttac agccaaaaag tttttggtag gggtagcatg tttttgcata acccacctgt
    24841 ggaaaactgt ggaataatag gaaagaagga ttgttggtgt tgacccggct ttagttcgct
    24901 tccttctcgg cgccaattct atcaaactgc tctaaaaaac gtttaagcaa cctatagtat
    24961 aaaataaggt agagcaggat gtttgaaatc caaataattg ccacatttac ccagaaagtt
    25021 ccagcaaatt ttccaaatac cggcttaaca ggtgagtaga agtgtgcctt cacaaacttt
    25081 gtatcaggat aaaggaaaat gggatcaatt ttctgaataa gccttccttt atactcggta
    25141 attctttcca cttcacccgt gtttctgaca aaatctgtaa gactctcgtt gtagtattgc
    25201 cgtttaaggt ttacaaatgc ttcctgccca ccaggagcag cctgaaagct ggatatgtag
    25261 gcatccttct ggctactggc tttgttataa agcttcacgt agtacttgcg aaccagctcc
    25321 aggtagcttt tcagctcgtc gagcaactcg ggtgaaactc cagccatgga aacctggtta
    25381 tacttactaa aagtaagctt cgagccctgc ggcgatgtca tttcatgcat aatttcgtta
    25441 cgcagcaggg ttagggcatc ttccacctcc tcactttttt cgggcaagcc caggttccga
    25501 gagcagaagt caaccttatt ctgaagcgat tttaaccagt agttcctctt gtagtccgaa
    25561 atgctcatgg cctcatcaaa cacgtagaag ggtttctcat acgggttatt acagaactgc
    25621 tcaactgcca gtgcctcgta ggcccatcgg gcaacaatca gctccccgta aaatggaatg
    25681 ctacttggtg aggatatttt gggattaagc ttttcgtact ttacaattac cccgctcaag
    25741 atgatctggg gaataaccaa aaacgggatg agaatataaa ttgttacaac cgtcttgaag
    25801 ctatccgaga tgaccaaccc cagcatgttc gacgagaacc atgcgctgaa tagaaccagc
    25861 cagtactcga agtacatccc gcgaacctca aggattgaat tcccaacaat tacaaacatg
    25921 aatgcctgca ctgccgacaa caccagcagt atggcaacct tcgacatcag gtagctggac
    25981 cagctgaggt tcaggaatga ttcgcgtttc tgaatttttc tatccttaat aatctcctcg
    26041 gcgcttaccg tgaggcctac gaaaactccc acaatcaccg acatgaacag gtaaacagga
    26101 atgttgctgt tttccatgaa ggtgtagcca accctgtttg aaacatcaat gttgaagtac
    26161 ttgataatgt aggacagtag gaaggccaac agcggtgttt ctagcacatt gataatcatg
    26221 tactgcgagt tggccagctt ggacagcacg tcgcgcttga caaaaatttt cagctgctta
    26281 aaccaaccag gggtcttaaa gtgtattttg ggaaggaaat cgggaatacc gtctagctgg
    26341 ggttgagctg aaaaccactt tttgtagtgg ttgttccact ctacggggct tattttacgg
    26401 gcacgggtag gattgccgta ctcgtcgagc acgttggcct ccacaatgtt gaatatttgt
    26461 tcggggttca cattgccaca ggtggcgcat tcgctctcgt tccagttggc atgctgaacc
    26521 cttgacttaa agtacatgat ggactcgata ggatcgccat tgtagatgag gtagccaccc
    26581 gtgtcgagta tcaggagctt gtcgagcatc ttgaatattt cggacgaggg ttgatggatt
    26641 accacgaaaa caagttttcc cttcagggta agttccttga gcaggtcgag gatgttttcc
    26701 gaatcgcgtg agctgagacc ggaggttggc tcgtcgagga aaaggatggc aggctcccga
    26761 ataagctcca gggcaatgtt gagccttttt cgctgacctc cgcttatctt tttgttaagg
    26821 ggtgtaccca cccgcatatc cctgatttca ttaagcccaa ggtttaccag tgttcgggaa
    26881 accgcctcat cgagctgctg gggcgtgtag ttgtcgaaac acaagcgggc gttgaaatac
    26941 agattttggt aaacggtaag ctcctcaatg agcagatcgt cctgtgaaac gaatccaatt
    27001 tgtccctcca ccaactcggg ttgcctatga atgtcaatgc cgttgatgag cacatttccg
    27061 gctgtgggct tttcgcttcc gttcagcacg ttcagcaagg tagattttcc tgcacccgag
    27121 gctcccataa tgcctaccag gtgtcccgat tcctcagtaa acgtcatttg gtgaataccg
    27181 atttttccac ttctgaatcg gtactcaacg tctaaagcct cgaatactat cctctccttg
    27241 agcttatcga gggcaaaccg cccaacaata tcgctgaagt aaaccggttt gattttagga
    27301 ttgcgaatgg aagagccatt gctcagaaca aaaattttct cgttctggat taggtggccg
    27361 tttaggtaga gctcatgggt gcccctgtaa cgcaggaaaa atatgttggc cgactcaata
    27421 aaaacaaccc gcagctcgtc tagcaagccc tcagcgtaaa tgtgcttcac accaggcagc
    27481 tcaaaatcct tgttgccgtc gatgagtaaa acatttgggg taataggaat ttggttgaag
    27541 ctggagagaa cgaaatcgga aagcatgttg aactcatcag cggaaacgta gaatgtttca
    27601 gcaacggtag aaataaatgc catttcctgc tcggtaacct tgccctcttc gggcttaaca
    27661 aactccagca gcctaaccag caccactatt ttttgctgct gctgtagctc ttcgtttatc
    27721 tgggcacaaa ttttcagcac ccttaccgag cttgacgatg ttcttttttt ggacttggtc
    27781 ttctcgcttt gcttctcctg gtaaagattg tagtagttgt cgaaaacctt gatgtactcg
    27841 gccaccagct cctggttcag ctggttacga aggaattcct ccactacccg acgacggtcg
    27901 ctgtaatcat tggcttccat ttcctcctct gcaaccggtc gggaaatgat ggcaaaaagc
    27961 tgcattagag cttttagaat cgattcgctc atggagtgct atataaggtt agaactactg
    28021 tttaaaccca ataagcagca taacatatcc aggtgtaaat tgctggctta ccacatccac
    28081 ctgaataata catataaccg ttgaagtgaa gcggaaattc cagtatggtg aatagtcata
    28141 atccctgttg gagaaaagaa ggtttttatc ggtgtcgtac acagttagaa caacctgccc
    28201 ctttttaacg cttgtgcagg tggtaatcct gtaggtattt tctccaaaga aagtggtgcg
    28261 gaactcgata acctggtcgg gcttaaggga ggcaaagtac tcctgaccat cggaaacgta
    28321 ttcgggagga aagttcagtg cacaggtgtt tctgaggttt tctacctggg cggtggcttt
    28381 aactgagaaa agtccgatta gtatgaaggg gattaaaatt tttttagcca tggcaacata
    28441 ttgtcattca actaatttat tgcggatact ttcaattttg ctgcttatga tccggatatg
    28501 gtactccgaa accaccacct gcgtggtgct atttattgtg atttgcctgt tggcagcatc
    28561 aataatcggc tccttgtagt tgtaggagta aattatgcca tcgtattcgt aggcaacatt
    28621 gataagctcg tctatcagct cggaaaaatc ggacgatttg tagtagtagg gagttaacag
    28681 ctcaataaga ttatcaaggg gatgtttctg ctcgccaaac cggttaatga tgtcccggtt
    28741 gctgtttcca agagcaatct gggtcagaaa gtacatgctc tcaatccacg aaccggcaac
    28801 tattaaggcg ccaatatcgc tccgctcgtt ggtcttaagg tataaatcgg aacttgagta
    28861 tgctttggtt ataattcgag ctaatgagtc cttgttgtta atattctgct caaccgcatc
    28921 aaaaaaattc tggtcgaaaa tttgctccag accaagctgg tcgcagagct tacgtatggc
    28981 atcgaggtat ttaatggctt cctgtgttct atcgtatatg cacaggtagc tgatatcggc
    29041 accgtaaatg ccaaggttaa gagcctgctt gaaagatttg ccgtactttt gataccggtc
    29101 gtaccggttg agcagattct cgttgtaagg aatgcttagg ttctttacca gccctacaac
    29161 ctgccaagga gagggaatgg gaaaaataga actttcatac ttgataaggg tgggtagcgt
    29221 atcaatactt acatcctctt taatcgtttg attatcagac ttattaggat gattacattc
    29281 agtaagccca aatattaaaa aacaacccga tacaaaaaaa actatgcgct taaaattaag
    29341 catacatcac ttttgaaatt gttcagtgca agtaagataa aataaatgaa atatccaaag
    29401 ggcaaaagcg gcaaattagc taccccaaca aacaacgggc aacaacctga tttggtaaaa
    29461 aaatattaaa cctcccggag cagcaccatg tcaaaataaa aggattattt ttgctttagc
    29521 ttggtgtagg atacccgaat cgcacacact aaatgctgat acaaacacta acttataaag
    29581 ttaaaagctt gaaatatgaa aaaagttacc ctccttacag ctgcatttat gctgtcgcta
    29641 acatcggttg cccagcagaa agttttgacc cttgaacagg cagtcctaag gcagtatgcc
    29701 gactttagac cccaaaccct ggcaaatttc agctggaagg ctaatgtcaa cgcatacacc
    29761 tacactgaag gcgacagcat tattgaggtt tccgttccaa gcggtatgcc taaaaccgtt
    29821 atgacgcttg aattgctaaa ccgtttgctc tcagccgatt caattcctgc actgaagagg
    29881 ttggcctaca catgggcatc ggaaaacgtt atcaaggtat tctcggctcc atacttggcc
    29941 tttgttgatg ttggtttggg gaaaatcaca cgtaggttta caattcccga agaggcggaa
    30001 aacctggagt taagccctaa caataccagc cttgcctaca cagttgcaaa caacctctac
    30061 attactgatg ctaagggtac tccggttgcc gttacaacca acgataaccc gggcattgtt
    30121 aacggacaag ctgttcatcg gaatgagttc ggaatcaaca aaggcatttt ctggtcgccc
    30181 agcagcagct acctggccta ctaccgcatg gacgaaacaa tggtaaccga ctaccccttg
    30241 gtggacataa cgcagcgcat tgcaagggta gagcctgtga agtaccccat ggccggcatg
    30301 aagagccacg aggttaccct cagggtttac aaccttagca cccattcgaa catcgaaatt
    30361 aaaaccgggc tacccgcaga acagtacctt acctgcgtta cgtggagtcc ggatgaaaag
    30421 tcgattttta ttgcaacgct gaacagggaa cagaaccaca tgcggctcaa ccggtatagc
    30481 gctcaaaccg gagagctgat ggcaaccctt tttgaagaaa ccgattctga atatgttgaa
    30541 ccccaacaac cgctggcgtt tcttgacgat aattcgggta ggttcatctg gcaaagccgg
    30601 cgcgatggct ggaaccacct ctacctttac gatgtaaacg gaaagttgct caggcagcta
    30661 acccgtggcg agtgggaggt taccaatata aacgctattg atcttccaaa cggtaaaatt
    30721 tacattaccg caactcgtga aagcccaata gaaaggcaag tttacgctgt tgacctaaaa
    30781 aaggacaaca ttaccatgct aacccagggg gcagggtttc ataacgcact tttcagtccc
    30841 gattgtaagt attttgtcga tttctactcc tccatttcca ttccaaacag ggttaacctt
    30901 tgcacgggca agggaaaaat agttaaaaac ctggtcaatg cccccaaccc tctggccagc
    30961 tacaacgtat cccttcccga actcctcacg cttaaggcta atgacggcac aaccctgtac
    31021 ggccgtttga ttagacctgt aaattttgac tctacactaa ggtaccccgt ggtggtttac
    31081 gtttacggag gccctcactc gcaactggta cggaactcgt ggatgggtgg ggcaagcctt
    31141 tgggagtgct acatggcttc aaggggctac atccttttca ccctcgataa ccggggaact
    31201 tcctaccggg gaagcaattt tgagcaaaca atccaccgcc ggctaggaac catagaaatt
    31261 gccgaccagc tggctggggt aaacttcctg aaatccttgc cctatgttga ttccacaaga
    31321 attggtgttc atggatggag ttttggagga ttcatgacca tttcgctcat gctgcgtgca
    31381 cctgaattct ttaaggttgg agttgctggt ggcccggtta ccgattggaa gttctacgag
    31441 gtgatgtatg gtgagcgtta tatggacacc ccacaggaaa accccgacgg gtatagcacg
    31501 tccgacctta aacagtacgt aaaaaactta aagggaaagc tcctcatcat ccacgacgat
    31561 atggacaaaa cggtggtgcc tcagaacagc ctcacgctgc ttcacgagtt tgttaaaagc
    31621 aacgttcagg tcgatttctt cctctacccc cagcacgagc ataacgtgat gggtaaggac
    31681 agggttcacc tgattgataa aattatccgg tacttcgacg acaacctgta aaaccagcta
    31741 ccctggtttt tgctacaagc tgtgcagagc agatgtgaga tgttccctta agg
//
LOCUS       DAOSHM010000027        31701 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_1102_length_31701_cov_20.789863, whole genome shotgun
            sequence.
ACCESSION   DAOSHM010000027 DAOSHM010000000
VERSION     DAOSHM010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 31701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31701
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1102_length_31701_cov_20.789863"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(422..913)
                     /locus_tag="PK839_05870"
     CDS             complement(422..913)
                     /locus_tag="PK839_05870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72299.1"
                     /translation="MEKGAKGNITGNQLEVAVKTVLTGKGFELINYRVWDKNKEKYGE
                     ELLLENVPFTTIYNHKGNTEFLLISKRFNLQIRIECKWQQVKGSVDEKLPYLYLNTIE
                     AMPENTIMILIDGAGWKTGAVKWLKDAVQQKKYTTAETQNKEIIVFTMTDFFTWANNT
                     FNK"
     gene            complement(914..1903)
                     /locus_tag="PK839_05875"
     CDS             complement(914..1903)
                     /locus_tag="PK839_05875"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: protein motif:HMM:TIGR00571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HOG72300.1"
                     /translation="MTQAIKYKIKDDNVSTSEDLFNHFNVGAKPFLKWAGGKGQLLDK
                     FQEFYPKLLKQKEIKTFYEPFLGSGAVFFDIAQHYDIEDAYLYDINEELILTYKVIQK
                     DVTKLLDFLYQYQKKYIKLDKKQRQVFFYDQRANFNLQRSNINFDKYSEQWFPRAAQL
                     IFLNRTCYNGLYRVNSKGEFNSPAGDYDNPTICDEQNLLAVHQVLKIAVIKKADFKEI
                     INDIKAKSFVYFDPPYRPISKTASFKAYSKQDFAENEQIQLAKLFRQLDNKGTHLMLS
                     NSDPKNYDPSDNFFDDIYRGYNITRIPAKRMINSDPTKRGAINELVVTNYQIA"
     gene            complement(2197..3036)
                     /gene="prmA"
                     /locus_tag="PK839_05880"
     CDS             complement(2197..3036)
                     /gene="prmA"
                     /locus_tag="PK839_05880"
                     /inference="COORDINATES: protein motif:HMM:NF001785.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006479 - protein methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HOG72301.1"
                     /translation="MAAFTELRIKTSPFSEETNEIIIAQLAELGFDSFMESESELLAY
                     SSIHSVSAAINTLHSIAIPAGVSIDYSAGETGERDWNEEWETGFEPVTVDGRCHIRAS
                     HHPVNPAAEYNITINPRMAFGTGHNPTTYMMLEALMDMNVEDCRVLDMGCGTGVLAIM
                     AELRKARSVTAIDTDEWAYRNTLENIRINGCSRVEVLLGDAQLLANREFDLVLANITL
                     NVLLANLPAYAQSLVCKGHMLLSGILTTDVDRLLQYAKSIGLEPVATRTREGWAMVQL
                     HRS"
     gene            complement(3036..3791)
                     /gene="tpiA"
                     /locus_tag="PK839_05885"
     CDS             complement(3036..3791)
                     /gene="tpiA"
                     /locus_tag="PK839_05885"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008860706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HOG72302.1"
                     /translation="MRKKIVAGNWKMNTSLAEGFSLAKEVIAGSSRVDQGVELVVIPP
                     FTHLSEIVKLARGSRVRVGAQNCAQWPKGAYTGEVSASMLQSAGVEYVILGHSERREY
                     FNETNRSLFEKLQLALQENLTPIFCCGEKLGEREAGQHFAVVEKQVAESLFGLETRQV
                     QQVVIAYEPVWAIGTGKTATADQAQEMHAFIRGLLAGKYGSTLADSISILYGGSCKPS
                     NAAELFAKPDVDGGLIGGASLVAADFIAIAGSF"
     gene            complement(3855..4937)
                     /locus_tag="PK839_05890"
     CDS             complement(3855..4937)
                     /locus_tag="PK839_05890"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG72303.1"
                     /translation="MIRFVIKRILYFILVVFGVVTLIFALFHMVPGDPARMVMGQRTD
                     SASLAAARADMGLDRPVAVQYLKYLNDLSPLSVHNPNNPKSYIYLDTLLYSGIVNLVK
                     LSDNRQLVLKLPYLRRSYQSRKPVTSIIAETLPNTFILALVAIIFATIVGIAMGIIAA
                     IRKDTAVDRMLLITSSFGMSLPSFFAAILVGWVFAYLLGSYTGLNLTGNLWEVDDLGE
                     GVHLQLKNLILPAFTLGIRPLAEITQLMRSAMLDTLNQDYIRTARAKGLKFRHIIMRH
                     ALKNSLNPVLTAISGWFASLMAGVIFVEYIFGWKGLGFVMVTALNSYDVPLVIGCVIS
                     ISLIFGVVNLIMDIIYSLLDPRVRMG"
     gene            complement(5001..5810)
                     /gene="mazG"
                     /locus_tag="PK839_05895"
     CDS             complement(5001..5810)
                     /gene="mazG"
                     /locus_tag="PK839_05895"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005831986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HOG72304.1"
                     /translation="MDKKTDNITRNERAREAFDRLLTIMDELREKCPWDRSQTFETLR
                     PLTIEETYELSEEILDKNFQAIKKELGDILLHIVFYSKMAAEENQFDMAEVINSLCDK
                     LIYRHPHIYGNVEVKDTNQVVENWEQLKITEKDGNKTVLSGVPASLPSLIKANRIQEK
                     VRAVGFDWEERSQVWDKVEEELEELKVEVERQNRNRIEGEFGDLLFSVVNAARLYNID
                     PETALERTNRKFMKRFGYLESKTIKQGQSLKDMTLDQMNAIWEESKQFDTE"
     gene            complement(5833..6678)
                     /locus_tag="PK839_05900"
     CDS             complement(5833..6678)
                     /locus_tag="PK839_05900"
                     /inference="COORDINATES: protein motif:HMM:NF014003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HOG72305.1"
                     /translation="MLIFNIPDYSITFVMLLANPMDNLRIVTLTTDWNQNDYYVGILC
                     GRLLSIEPTLRIVELSHRVPPFDYIQAAFIMRGCFENFPPGTIHICMVNSDSTTSKQL
                     LLFELKGHFMIVPDNGIVGLITEVAPDRVYSIPFEEHSSFGSLSAVASAMNMLLFNSN
                     LADIALIAGTYKQNINIRAASDESSITGSVIYVDSYQNAITNITREQFEQVRKGRKFS
                     IYVQSFSYKIEEIVENYADVEDGDMLALFNSLNLLEIAIRNGFLTELLALSVGSSIMV
                     KFTNR"
     gene            7099..8106
                     /locus_tag="PK839_05905"
     CDS             7099..8106
                     /locus_tag="PK839_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010264372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOG72306.1"
                     /translation="MVGERIITVEGVDPLAIYGVNNTRFEMISRHYPKLKLVARGNQI
                     KVTGDEELIDDLESKINRMVEFYSRYSRLTEANILDFLNDNDSSIVKAEEDALDVLVY
                     GTNGRTVKARTINQRKLVELYQTNDLLFAIGPAGSGKTYTAIALAVRALRNKEVKRIV
                     LTRPAVEAGERLGFLPGDMKDKLDPYLQPLYDALHDMIPQKKLMEYMDDGTVQIAPLA
                     YMRGRTLDNAFVILDEAQNTTLNQLKMFLTRMGKNAKFIVTGDITQIDLPNRGDSGLV
                     KAIGILENIGSIAMVYFDARDIVRHKLVKHIVNAFERYTSGNGSSERTENNDECEDNS
                     Q"
     gene            8278..9225
                     /locus_tag="PK839_05910"
     CDS             8278..9225
                     /locus_tag="PK839_05910"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HOG72307.1"
                     /translation="MEAIVRTDFKFPGQTSVYHGKVRDVYNIANKHLIMVATDRISAF
                     DVILPKGIPYKGQVLNQIAAKFLDATADIVPNWKIATPDPMVTVGRLCEPFKVEMVVR
                     GYLTGHAWREYKSGNRLLCGVPLPEGMREHQKLPAPIITPTTKAIEGHDEDISREEII
                     ASGLVEEHDYAQLERYTLALFERGTRMAEKMGLILVDTKYEFGRADGRIYLIDEIHTP
                     DSSRYFYADGYTERFNRGEEQRQLSKEFVREWLMANGFQGQEGQKVPEMTPEFVNQVS
                     ERYIELYEQITGEKFQRAEGSDVMKRIERNILNFLKTLV"
     gene            complement(9312..12200)
                     /locus_tag="PK839_05915"
     CDS             complement(9312..12200)
                     /locus_tag="PK839_05915"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOG72308.1"
                     /translation="MPATLKPFLALTLQHHRQLGTTIGAYLATPTESHGIYIISENAA
                     VAQPSDLEDIVSPIQKDMLQQLQQISDKALQKYFDKQAKSVAEFLDSLTRSEQLNSHV
                     HSYVERRIAKVVALAAHSDTPIFLRKKEEKNLYEENRLNVELEPTLPLFRFELTPDSI
                     MYSIMAMNNGRTVNLTTTNGEVVTNNPCIIRIDRTIYQFEAIDGKKLTPFLSKPYITV
                     PKSAEKKYMQTFVLNAIRHQQVEAVGFNIVDEQHDGRAILTLEERLAGGWCLTLKFSY
                     GDRGYLCNTPYNTEVRLVEEGNGYTFRRFNRNRIWESRVICLLSEKFNLQRVSEAEFL
                     PAAVENAKENLYPLVEWLNQHVNQLKTEGFAVAQLVRGMNFYLNGVVVAISTDSTTDW
                     FDLYGKIKLDGFEIPFVYLKRNILKNIREFLLPNGQVFIIPEVWFSRYKPLMLIGKEE
                     NEGIRLPKSLFGLLADTNEVNSPAASELRHRLSNLKKEGIQLPQRLNARLRSYQLVGY
                     AWLKLLNQNGINGCLADDMGLGKTIQALTLLQSINEEQNYATGMGKTSLVVVPTSLVA
                     NWISEAGRFTPELKVAAYMGQQRVKSASWLLNHHVVVTTYGLIRNDIDMFREVTFRYV
                     VLDESQTVKNPLTKGYRSVMQLRAINYLSLSGTPVENSLLDLWSQLNFLNRGMLGGLR
                     AFRNEFQTPIERQGDHEKEKQLKQLIEPLILRRTKEEVAKDLPEISEQVVYCRMSDSQ
                     KEIYEKEKSVVRNFILETLDSPAPNQSTVSILASLTRLRQIANHPAMLPEYRLADSGK
                     FDEVAMGIESATAGHHKILVFSSFVRHLEQVEVFLQDRKIGYEKLTGSSANRSEIVER
                     FQTDPDKKVFLISLKAGGVGLNITAADYVFILDPWWNPAAEMQAASRSHRIGQDKKVF
                     VYRFITSETVEEKILSMQERKENLAQLFVSSSSSLGQISREAILELLE"
     gene            12835..14721
                     /locus_tag="PK839_05920"
     CDS             12835..14721
                     /locus_tag="PK839_05920"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOG72309.1"
                     /translation="MRSYITALLLCLSPMLLAGQVVEGDIPSVQRSTDKVKIDGKNYY
                     IHIVKSGETLYSISKAYEVSQSDIAVNNPDIYNGLKIGQALKIPVRASESKGTDDYIY
                     HIVAKGETLYSISRRYNIPVDVLTSINPEVKDGLLVSQVILIPRNPMIPEAKKPAADT
                     VQFIMHEVQKGEGLLAVARRYNIDAKTIETYNQEVLKDGLKLGEYLRIPAVKQHPADS
                     LMVTSKPEPVAFFSTCKNNFRYNGQPFDISLLLPFSQGSAVDSDELIDEAEEVKMDPG
                     TTNTLESSRITSQETLASLNFYEGFLLAVDSMKKAGVSMNINVFDTKHQPEEVTSILN
                     KGKLDKSSIIIGPLFVNEIKPVVAYAERKSIPMVSPLYSGNEVISEYPGLISVNQGYG
                     EQLEVFIKNFKFSDTCHYVVIYDMEKLLTGPIQNFSSALNQRIRESGVRVDSVVHQTA
                     AMKSEEVQAKLHSYMKADGCNVVIIPSQDEPFVSDLLGQLYAVKSLNGLKMIVYGPAR
                     WQKMKNVPPDYLYKLNARVFTPFYVDYSRDEVKRFVASFRDRYRAEPNQFSFLGYDVG
                     IYFISALKTYGPDFTKCLKDFQKDMLQSTFTFDVVSNESRLSNTHQFIIGYTDDYDLV
                     RIIP"
     gene            15045..16664
                     /gene="pckA"
                     /locus_tag="PK839_05925"
     CDS             15045..16664
                     /gene="pckA"
                     /locus_tag="PK839_05925"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016274536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HOG72310.1"
                     /translation="MANLDLSAYGVNQPAEVFYNPSYELLYQHETDPSLQGYERGIVT
                     KLGAVNVDTGVFTGRSPKDKYVVKEPNTEKNVWWAQPGKKSSDNKPITQEVWNHLKDI
                     SAKQLSGKKLYVVDAYSGANEDTRLCVRFVMEVAWQAHFVKNMFIRPTDEELKNFKPD
                     FIVLVASKTSNPKWKEQNLNSEVFVAFHLTERMALIGGTWYGGELKKGIFSVMNYFLP
                     LKGIASMHCSANVDQKNGNVAIFFGLSGTGKTTLSADPQRALIGDDEHGWDDNGIFNL
                     EGGCYAKCIDLDPEKEPDIYAAIRRDALLENLDVDSEGNIDFHSKVKTENTRVSYPIY
                     HIQNIVKPVSKAGHAKHVIFLTADAFGVLPPVSRLSPEQMQYHFLSGFTAKLAGTERG
                     VVEPQPTFSSCFGQAFLMLHPTVYARELVKKMQLHGSKAYLVNTGWIGGPYGVGKRID
                     LPSTRAIINAILNGSIEESEFELLPVFNLQVPLRVNGVNPHLLNPQKSWANPGDWDKA
                     ARNLAGMFIKNFENFTDNEWGKSLVKAGPHI"
     gene            17068..17724
                     /gene="plsY"
                     /locus_tag="PK839_05930"
     CDS             17068..17724
                     /gene="plsY"
                     /locus_tag="PK839_05930"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010264348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HOG72311.1"
                     /translation="MEILSKIAIVFLAYLMGSIPTSVWVGKIFYGVDVREHGSGNAGA
                     TNTIRVLGLLPGIVVFIVDVLKGYLAVRLLYLTDFYIPKTGMFINFQLILGIAAMIGH
                     IFPIFAQFRGGKGVAVLSGVVFALHPYATLIVFGIWIVSVIITGYVSLSSMIAGFSFP
                     LILIFVYRTTNPSLILFAFILAILMLFTHQKNIERLFKGEESKFHLKKKPHNEPDKPK
                     "
     gene            17786..18502
                     /locus_tag="PK839_05935"
     CDS             17786..18502
                     /locus_tag="PK839_05935"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005677448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HOG72312.1"
                     /translation="MQIFYAPNITGSSYTLPEDEARHCLRVLRMRVGDEVELVDGKGN
                     LFTGRIANPDLKECTLEIVRKASNFGERHFYLHIAIAPTKNADRFEWFAEKVTEIGVD
                     EITPLLCERSERKSVNSERIERIVISAMKQSEKAYLPTLNTMTSLEKLVASTPVDFVK
                     LIAHCNEKGIPHLKKMVKPGDKVLILIGPEGDFSPKEVDFALINGFRAISLGNSRLRT
                     ETAGIVACHAVNMLNTEDFS"
     gene            18533..19339
                     /locus_tag="PK839_05940"
     CDS             18533..19339
                     /locus_tag="PK839_05940"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG72313.1"
                     /translation="MRRLLVGGYRFSWFHIAAALLGGVVLLFIVAPILGMIISTPAKS
                     LIETAAEQEVVRSIWLSVWVAMAATLVFALFCIPLAYLLARFDFPFKRLVLGIIDLPI
                     VIPHSAAGIAVLGLIARDGLLGRLTSPLGIELVGSPAGIAIAMAFVSVPFLINAAREG
                     FALVPERLEKAALSLGASPITIFFTVALPLAWRSVVSGLVMMFARGMSEFGAVVIVAY
                     HPMVAPVLIFERFGAFGLKYAKPVAVLFIGVSLTVFIIMRLLSRNRSNHA"
     gene            19332..20396
                     /locus_tag="PK839_05945"
     CDS             19332..20396
                     /locus_tag="PK839_05945"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOG72314.1"
                     /translation="MLRVEGVCKKFQDFSLNQISFTVDQGDYFMLLGPSGAGKSLILE
                     TIAGLHKPDSGKIFFQGVDITHIGVRERNFGIVYQDYAVFPHLTVYGNIAYPLRRKGI
                     RGKELHKQVTRFAERVSVAHLLARHPQTLSGGEVQRTALARTLAMEPRILLLDEPLSS
                     VDVKLRSELRSLLRSINSSGQTVIHVTHDYEETLALASHLAVVNGGCIEQVGSVQEIF
                     QNPRSEFAAMFAGVHNFYPASIVEFSEHGLTMVRLNDTIAICCYTEGCNGEGFVCFSE
                     ESVILSTEKPHQSALNVFRGRVVEIFPQRFGLMAVVDAGFRVLSKVTWESASRFGLTP
                     GVEVWVSFKASSVRFIPRAS"
     gene            complement(20398..21249)
                     /locus_tag="PK839_05950"
     CDS             complement(20398..21249)
                     /locus_tag="PK839_05950"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HOG72315.1"
                     /translation="MRQSGVRCIIFLLLPLVVVSCLKPGSYNIRERYVVKAKTLIVRS
                     SPSTASAPVVTLQQGDTINAIVSDTEWVTVKVGTKKGYVAASYLNRIEPTPTPNLLSW
                     VEKTASWESWVFWLIAIPAIVIWIFSGKLIVQYKNRLAQKHDFQVKGLVLMPVVFFIN
                     ALLLAILYINWKDLLLNSIEHGIRAIPNNPDIIDWVVWCQALSLLVSILVDLLGTVIK
                     SGILWGVVLTFVDLLWSVFIFAATFFLTLSLYYYAIVFLLIFFGIQYVSMVYQNSRKI
                     SSYSRPR"
     gene            complement(21486..24104)
                     /gene="alaS"
                     /locus_tag="PK839_05955"
     CDS             complement(21486..24104)
                     /gene="alaS"
                     /locus_tag="PK839_05955"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HOG72316.1"
                     /translation="MTSAEIRRAFLNFFEGKSHTIVASAPMVVKNDPTLMFTNAGMNQ
                     FKDIFLGNTPAKFKRVANSQKCLRVSGKHNDLEEVGHDTYHHTMFEMLGNWSFGDYFK
                     NEAIDWAWEFLVDVLKIDSTRLYATIFEGSPDEGVEKDYEALNCWAKHLPSNRIIEGN
                     KKDNFWEMGETGPCGPCTEIHIDLRGEDERKANPGEKMVNTGHPLVIEIWNLVFIQFN
                     RKANGQLEPLPAKHVDTGMGFERLSMVVQGKKSNYDTDVFKPLIDRISNISGLKYGAD
                     AKSDVAMRVIADHLRAICFAIADGQLPSNVKAGYVIRRILRRAVRYGYTFLNFREPFM
                     AGIIPVLTEQMGDQYPELRKQQSLITSVITSEETAFLRTLETGMRMLDQLVERARKEH
                     KMEISGKDAFVLYDTYGFPLDLTELILRENGLSVDHSGFDGEMALQKERSRADAAVEE
                     MDWIVVNDDENAEFVGYDTLTCNVHIARMRTVRVKSNEQYQLVLDKTPFYAESGGQVG
                     DSGTITGEGEAIRVLNTIKENNLTIHIVDKLPANPNGTFVASVDVNRRASTAANHSAT
                     HLLHNALRKILGLHVEQKGSLVHPDYLRFDFSHFQKMSEDEIRQVERLVNSVIRENVH
                     LDEKRNMPIDEARSMGAMALFGEKYGDRVRVIHFGESVELCGGTHVEATGRIGFFKIL
                     SETAIAAGVRRIEAITGIKAEEFVYSQEDTIKEIKSLLGNPSNVSQTVARMVADNEML
                     RKEISSLQAEKLAEVKHQLKQSIEQRNGINVICQKVGLNDSTTIKDLAFQLRNEVPNL
                     FLAIGATASEKAILTVMISDNLVQSLQLNATSIIREAAKEIQGGGGGQPFFATAGGKN
                     PAGLDAALAKTLEFLE"
     gene            24255..25217
                     /locus_tag="PK839_05960"
     CDS             24255..25217
                     /locus_tag="PK839_05960"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOG72317.1"
                     /translation="MVKVKYHFNPHTLKFDAVSLPFYKRLAKILIHLGFYLLVFVVLG
                     YGFSMVFDTPIAQGLKRTNSEYSLKYEMALRQMQEFNDILTEIEQKDNNIYRAIFECD
                     SIPFSIRRGGYGGSERYSELVNNSNSHLLVKTFKILDELSWRAYIQSRSLDEVTELAI
                     NKERMIECVPAIQPISVKNLVRISDFFGYRRDPITRAPTMHEGLDFAGPIGTPIYSTG
                     DGIVIEAGYSFNGYGNQVMIDHGFGYKTRYAHLSKVNVKVGERVKRAEVIGKLGNTGK
                     STGPHLHYEVIVRGKPVNPINYFNDMTEQDYEAMLKNYSSQFLD"
     gene            25235..26206
                     /locus_tag="PK839_05965"
     CDS             25235..26206
                     /locus_tag="PK839_05965"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOG72318.1"
                     /translation="MAKYKYKFHPEQLTFVRVRATLKEKLFVALKYMVATAIVGIISY
                     LVFPFFIDTPEVRKLKRENQELMLNFELIDKRLDQLRAVIENLQQRDDNIYRIIFEAE
                     PIHPSVREAGMGGVDRYSYLEGLSNNKIIVETARKIDLLSKKAYIQSRSFDEISTLAK
                     RKEEMVRCIPAIMPINNKDLTRMASSFGMRIHPFYKVLKMHTGMDFTSPLGTEVYSTG
                     DGVVTNVEYAQRGYGYNIIVDHGFGYKTRYAHLGKIIVKPGQKVRRGMVIGLVGNTGT
                     SVAPHLHYEVIRNNTPVNPINYYFNDLTPEQYDKLVEIASQPTQSFD"
     gene            26224..26562
                     /locus_tag="PK839_05970"
     CDS             26224..26562
                     /locus_tag="PK839_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005925825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOG72319.1"
                     /translation="MPYKEKNIEKFYFSIGEVAEMLGVNASLIRFWEKNFDIIQPRKN
                     QKGTRFYTKEDIDNLKLIYHLVKEQRMTIEGARKKIAEDKHSVSDSFKIIESLNSIKQ
                     MLLEVRDQLD"
     gene            26590..27684
                     /locus_tag="PK839_05975"
     CDS             26590..27684
                     /locus_tag="PK839_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010538027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HOG72320.1"
                     /translation="MKLDEIVSYFEELAPTAYQEEYDNAGLIVGDASMEVKAALLTLD
                     VTPEVVLEAIDKGANLIISHHPIIFRGIKKFTGSSYVEKTVAAAIKNDIAIFAAHTNF
                     DSMMGGVNLSIARKLGITNLEILQPINNGLFKLVTFVPVDSVEKVRDAMFKAGAGQIG
                     GYDCCSFGSEGFGTFRAGDNTHPYVGQKGEMHREPEVRVETILPKNILARVVEKMIEA
                     HPYEEVAYDIYPLEIPYRQVGLGMVGNIEEPVASVDFLRQVKEVFGAGVVRYTALSRQ
                     VIKRVAFCGGSGASLIGKAIGSGADIFITSDIKYHQFFDADGRIIIADIGHFESEQFT
                     IELFYEYLLKKFPTFAVLKSRVNTNPINYL"
     gene            27704..28462
                     /locus_tag="PK839_05980"
     CDS             27704..28462
                     /locus_tag="PK839_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018710268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HOG72321.1"
                     /translation="MKNKPAEPTAELSVEEKLRFLYMLQQVDSKIDRIRMLRGELPLE
                     VQDLEDEIEGLETRIDNLKQEIKSLESEVSAKKQEIKDANTLIKKYQEQQKTVRNNRE
                     FDSLSKEIEYQTLEIELCEKRIKEFTQQVKDKTLVIEQSQKLLEERKLDLKGKRMELD
                     NIINETQKEEEELIRKSEEYGKIIEPRLYTAYRRIRGNARNGLAVVTVQRDACGGCFN
                     KIPPQRQLDIKIHKKIIVCEYCGRILVDDSLASM"
     gene            complement(28519..29214)
                     /gene="rsmI"
                     /locus_tag="PK839_05985"
     CDS             complement(28519..29214)
                     /gene="rsmI"
                     /locus_tag="PK839_05985"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HOG72322.1"
                     /translation="MSKLFIVPTPIGNLGDITLRALEVLRSVSLVLAEDTRTSGNLLK
                     HYSIETPLRSHHKFNEHQSVEAIAEQIAAGLDVALISDAGTPGISDPGFLLVRTCLER
                     GVEVECLPGATAFVPALVQSGMPCDRFVFEGFLPQKKGRSKRIEALQDEDRTMVFYES
                     PFRLVKLLEQLMAVFGPDRRVSVSRELTKLHEETMRGTLAEVAAHFNSKTVKGEVVVV
                     VQGLGRNKSLDEE"
     gene            complement(29360..30100)
                     /locus_tag="PK839_05990"
     CDS             complement(29360..30100)
                     /locus_tag="PK839_05990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72323.1"
                     /translation="MQYLRDSKTLLDDSMKIIERALASENLPSIERNILLDKLAKAYE
                     LLLFSVEKQAEPTHQAHSQHTVKANAETTTQGIKRDQPLSTPSHNVEPVAPAGSYSKK
                     PPEQIQKFREKAPSTNESKSIEVEPTPGAASTDRPATLADKFQDKQKSLNETIANQAR
                     RSDMGTKLQNKPITDLAKAIGINDKFLFTNELFDGDSALYAETVNRLNEMDDLNEAII
                     YLQDNFSWSATSEAASKFIDLLRRKFAL"
     gene            30191..30778
                     /locus_tag="PK839_05995"
     CDS             30191..30778
                     /locus_tag="PK839_05995"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HOG72324.1"
                     /translation="MPFIENSVSNKRNGWIEVIAGSMFSGKTEELIRRLKRAKYARLR
                     VEIFKPQIDTRYSEEKVVSHDANSIISTPVTSSGNILLLAQDVDVVGIDEAQFFDMGL
                     PRVCTQLADMGVRVIVAGLDMDFRGKPFGPMPNLMAIAEYVTKVHAICVRCGELAHFS
                     HRTADTDKLVVLGETDIYEPLCRHCYNEALSKSKT"
     gene            30782..31237
                     /locus_tag="PK839_06000"
     CDS             30782..31237
                     /locus_tag="PK839_06000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HOG72325.1"
                     /translation="MAYREVSFRISRKNFWFITFLIVLLPISSKYRLLIFGKRTQGVV
                     VANEVKSSAFYQSHGYDSYPVIEFNADSLTLRMYGPENTNYEVGRRFTVIYNPDNPYN
                     CMILSLSYFYTGRGVILPGTIMLLWISFYLTFRSRDSKNNPRKGERDSP"
BASE COUNT         8355 a         7027 c         7326 g         8993 t
ORIGIN      
        1 ttaagttttc gcctactttt agtcttggtt ttgctggtgc accgtactaa gtaagtaagc
       61 caatttttgt caagcgaatt aggggggtat gcaatatttc agcttttacc aaaagaacat
      121 gcctacggca ttgaagctcc gtaggagcaa acttatggtt gaaagtaggt aaactagtga
      181 aaacaagttc cgtaggagcg gacttttcat tcataaaatt caaaaatata ttcctcctta
      241 tactttactt caaaatttct caactaatca aggtattcat ctcttaaatt tttcattgta
      301 aatgcaactt ttttgttaaa caatatttga tatagcttct ctttgggtgt taaaaatcca
      361 aaacgctttc ttggcctgtt gttcagtatc atttccaccc gttgcaaatg ctcattttga
      421 attatttgtt gaaagtattg tttgcccaag taaaaaaatc tgtcatagtg aatactataa
      481 tttctttgtt ttgggtttct gctgttgtgt attttttttg ttgaacagcg tctttcaacc
      541 acttaactgc accagtcttc caacctgcgc catcaatcaa aatcattatt gtattttcgg
      601 gcattgcttc aattgtgttt agatacaagt aaggtagttt ctcatcaaca gaacctttaa
      661 cttgttgcca cttgcactca atacgaattt gcaaattgaa tctctttgaa atgagtagaa
      721 attctgtatt gcctttgtga ttgtaaattg ttgtaaatgg aacattctca agtagcagct
      781 cttctccgta tttctctttg tttttatccc aaacacgata gttgataagt tcaaatcctt
      841 ttcctgtaag aacagtttta actgccactt caagttggtt tccagtaata ttacccttag
      901 ctcctttttc catttatgca atttgatagt ttgtaacaac gagttcatta attgctccac
      961 gctttgtcgg gtcagaattt atcattcgtt tagcaggaat tctagtaatg ttgtagccac
     1021 ggtatatatc gtcaaaaaaa ttgtcagaag ggtcgtagtt tttgggatct gagttgctta
     1081 acattagatg cgttccttta ttgtcaagtt gcctaaatag ttttgcaagt tgaatttgct
     1141 cattttctgc aaagtcttgt ttactgtatg ctttgaaact agctgtttta cttattggcc
     1201 tgtaaggcgg atcaaaataa acgaatgatt ttgctttaat gtcgtttata atttctttga
     1261 agtcagcttt cttgattaca gcaattttca aaacttgatg aactgctaat aaattttgct
     1321 cgtcacaaat tgtagggtta tcatagtcac ctgctggaga attgaactct ccttttgagt
     1381 ttactctgta aagaccgttg tagcaggttc tgtttaaaaa tattagttgt gctgctctag
     1441 gaaaccattg ttctgaatac ttatcaaagt tgatattgga gcgttgtaaa ttgaaatttg
     1501 ccctttggtc atagaaaaat acttgacgtt gcttcttatc gagtttgatg tatttctttt
     1561 gatattgata aagaaagtca agaagttttg ttacatcctt ttgaatgact ttgtaagtca
     1621 aaatcaattc ttcgttgatg tcatataggt aagcatcttc aatatcataa tgctgtgcaa
     1681 tgtcaaaaaa aactgcaccg ctgcccaaaa aaggttcata aaaagtttta atctcttttt
     1741 gtttaagtag ctttgggtag aattcttgga atttgtcgag tagctgtccc tttcctccag
     1801 cccattttag aaaaggcttt gccccaacat tgaaatggtt gaaaaggtct tcagatgtcg
     1861 aaacgttgtc gtccttaatt ttatatttta tagcttgcgt catagtgttt aaaaccattg
     1921 tttaaattta gtcagaaaaa agagtaaagt aaaaaaattg agattatagt ttcgaccaag
     1981 cattttgcac tactcgttta tccccgccct tcatatggca ttacttccaa cttgggaagt
     2041 atagacgcta cgacattaca ttatttggta aacaaattta tggacttttc ttaaatcaca
     2101 aaaaatctaa aatatttcga cagatacctt ctccgatggc ctgagcatgt gtctcgaacc
     2161 tgtagctgag gttaatgggt ggttggtgta gttaacctac gaccggtgca gctgcaccat
     2221 tgcccagccc tcgcgggttc gggttgcaac gggctctagt cctatgcttt tagcatattg
     2281 cagaagccta tctacgtcgg tggtgagtat gccgctaagg agcatatgtc ccttgcagac
     2341 caggctctga gcgtaggccg gcaggttggc aagcagcacg ttcagggtta tgttggccaa
     2401 aaccaggtcg aattcccggt tggccagcag ctgcgcatcg ccgagcagca cctctactct
     2461 ggagcagcca ttaattctga tgttctccag ggtgttacgg taggcccact cgtcggtgtc
     2521 gatggctgtg acggaacggg cttttctcag ttcggccatt atggccagca cccctgtacc
     2581 gcaccccata tccagcaccc ggcaatcctc tacattcatg tccatgagcg cttcaagcat
     2641 catgtaggtg gtggggttgt gccccgtgcc gaatgccatc ctggggttaa tggttatgtt
     2701 gtattcagct gcggggttta ccgggtgatg tgaagccctg atgtggcacc ggccatcaac
     2761 agttaccggt tcgaagcccg tctcccattc ctcgttccag tccctttcgc cggtttcacc
     2821 tgcggagtag tcaatgctca cccctgccgg aatcgctatg gagtgaaggg tgttaatagc
     2881 agcgctaaca ctatgaatac tagaatacgc tagtagttca ctttcgctct ccataaagct
     2941 gtcaaagccc agctcggcca gctgggcgat gattatctca ttggtttcct ccgaaaaggg
     3001 agaggtttta atgcggagct ccgtgaaggc agccatcaga acgagccagc tatggctata
     3061 aaatcagcag caacaagcga tgcgcctcca atcagcccgc catccacatc gggcttggca
     3121 aaaagctcgg cggcgttcga gggcttgcag ctacccccgt acagtatgct aatgctatcg
     3181 gccagtgttg aaccgtattt gccggccagc aggccacgta tgaatgcatg catttcctgc
     3241 gcctggtcag ccgttgcggt ttttcctgtg ccaatggccc aaacgggctc gtaggcaatt
     3301 accacctgct gaacctgcct ggtttccagc ccgaaaagcg attcggcaac ctgcttttcg
     3361 acaactgcaa agtgctgtcc ggcttcacgt tctcccagct tttcgccgca gcagaagata
     3421 ggggtgaggt tttcctgaag cgctagctgc aacttttcaa aaagcgagcg gttggtctcg
     3481 ttaaagtact ccctgcgttc ggaatgccca agaataacat attcaacccc agccgattgc
     3541 agcatggagg ccgatacctc accggtgtag gcaccctttg gccattgggc acagttttga
     3601 gcacccaccc ttacacggct gcccctggcc agctttacaa tctcgctcag gtgggtgaac
     3661 ggaggtatca ccaccagctc aaccccctgg tcaaccctcg atgagcctgc aatcacctcc
     3721 ttggccaggc tgaatccttc tgccaggctg gtgttcattt tccagtttcc cgctacaatt
     3781 ttttttctca tggcatatta tttttaaggt ttcccgtcaa cagaatttcc agacaaacat
     3841 agcaaaaatt cctgtcagcc cattcttacc cttgggtcaa gcagggagta gataatatcc
     3901 atgatgaggt tgaccacacc gaaaattaag gagatggata ttacgcatcc gatgaccagt
     3961 ggaacgtcgt agctgtttaa ggctgtaacc ataacaaatc caagtccttt ccacccaaaa
     4021 atatattcca caaagatgac acctgccata agggaggcaa accatcccga aattgccgtt
     4081 aaaacagggt taagggagtt ttttagtgca tgtcgcatga tgatgtgccg gaactttagc
     4141 cccttggccc tggccgtgcg gatgtagtcc tggtttaggg tatcgagcat ggcgctacgc
     4201 ataagctggg tgatttctgc tagtggtcgg atacccagcg taaatgcagg tagaatgagg
     4261 tttttaagct gaagatgaac cccctcgccc aggtcatcaa cctcccaaag gttaccggta
     4321 agattaagcc cggtgtagct gccaagcagg taagcaaaaa cccaacctac cagtattgca
     4381 gcaaaaaatg agggcaacga catgccaaac gatgatgtta tcagcagcat cctgtcaacg
     4441 gccgtatcct tacgaatggc agcaataatt cccatggcaa tgcccacgat ggttgcaaat
     4501 attatggcta ccagtgccag tataaaagtg ttgggaaggg tttcggcaat aatggaggtt
     4561 accggtttgc ggctttggta agaacggcgc aggtagggga gtttgagtac gagctgtcgg
     4621 ttgtcggaga gttttaccag gttaacaatg ccgctgtaga gaagtgtatc gaggtatatg
     4681 tagcttttgg ggttgttggg attgtgaacc gacaggggcg acaggtcgtt gaggtacttt
     4741 aggtactgaa cggcaacggg cctgtcgagc cccatgtcag cacgtgccgc tgccagcgag
     4801 gctgaatcgg tgcgctgacc catcaccatg cgggccggat cgccgggaac catgtggaat
     4861 agagcgaata tgagggtaac caccccaaaa accaccagga tgaagtaaag tattctttta
     4921 ataacgaagc ggatcatttt gcgttgtaaa tgaggagtga ttaccttctg gttgatgggc
     4981 agcccaatgt ttattgctgg ttactcggta tcaaactgct tcgactcctc ccaaatggca
     5041 ttcatctggt ccagcgtcat atccttaagc gattgtccct gcttgatggt tttagactcc
     5101 aggtagccaa accgtttcat gaacttgcgg ttggtgcgtt ccagcgctgt ttcggggtca
     5161 atattgtaga gcctggccgc attgacaact gaaaacagga gatcgccaaa ctcgccctca
     5221 attcggttgc ggttttgcct ttccacttct accttcagct cctcaagttc ctcctcaacc
     5281 ttatcccaaa cctggctgcg ctcctcccag tcgaaaccaa cggctctaac cttttcctga
     5341 atcctgttgg ccttaataag cgagggaagt gaggccggaa cacccgaaag cacagttttg
     5401 ttcccgtcct tttcggtgat tttcagctgc tcccaatttt ccactacctg gttggtatcc
     5461 tttacctcaa cattaccgta gatatgcggg tgacggtaaa ttagcttatc gcaaagactg
     5521 tttataacct cagccatatc aaactggttc tcctcagcgg ccattttgga gtaaaagaca
     5581 atgtgcagca ggatgtcgcc aagctccttc ttgattgcct gaaagttttt atccagaatc
     5641 tcctccgaca actcgtaggt ttcctcgatg gtaagtggcc gtaaggtttc gaatgtttgg
     5701 ctgcggtccc aagggcactt ctcccggagc tcatccataa tggtcaaaag cctgtcgaat
     5761 gcctctcttg ccctctcgtt tcttgtgatg ttatctgttt tcttatccat attgctaaat
     5821 ttattgaact tgctagcggt tagtgaactt aaccataatg ctcgaaccta cagaaagagc
     5881 gagaagctcg gtcagaaatc cgttgcgaat ggcaatttcc aagaggttaa gcgagttgaa
     5941 gagagccaac atgtcgccat cctctacatc ggcgtagttt tcaacaattt cctcaatttt
     6001 gtagctgaag ctttgcacgt agatggaaaa tttccggccc tttcgcacct gttcaaactg
     6061 ctccctggtg atgttggtta ttgcattttg gtaggaatca acatagataa cgcttcccgt
     6121 tattgacgat tcgtcggatg ctgccctgat gttaatgttc tgcttgtagg tgccggctat
     6181 cagcgcaata tcggccaagt tggagttaaa aagtagcatg ttcatggcgc tggcaacagc
     6241 gctaagcgac ccaaagctgc tatgttcttc aaagggaatg gagtaaactc tgtccggagc
     6301 aacttcggta atcaggccaa ctatgccgtt gtcgggcaca atcatgaaat gaccctttag
     6361 ctcgaacagc agaagctgct tgctggttgt gctatcgctg ttgaccatgc aaatatgtat
     6421 agttcccggc ggaaagttct caaagcaacc ccgcatgata aatgctgcct ggatgtaatc
     6481 gaatggcgga accctgtggc ttagctccac aatgcggagg gtaggctcaa tggagagcaa
     6541 cctgccgcat agaatgccga catagtagtc gttttggttc cagtctgtgg ttaaggttac
     6601 tatccgcaaa ttatccatgg gatttgctaa aagcattaca aaagtaatgg agtaatcggg
     6661 aatattgaaa attagcatcc tatttcaata gcatcaccac gcaaatagca agtatccaac
     6721 cacataacta tatggactga ttttaaaaaa gaccagggaa tcattatgaa taacgtaggt
     6781 aaatcaagaa gtgcgttgga atgcctaaaa ccgaacctag acgccaaacg gtaagaaaaa
     6841 aatcaattat actaaaaacc tacactcttg cggaagttac attagctaaa aaattgattg
     6901 ctgaaacttt aaaaatagct gtataaaaat atttgcgcca gtggcacccc atttaacagg
     6961 aatgggttat atttgtgagg ttttggttat taacagtagc ttgtttacta aattcaatct
     7021 ggttgagaac tcaaatagcc ttacaatgtt ttgattatat tgcccagctc ccgttgggtg
     7081 gcaatcttgt gaaattttat ggtaggagag cggattataa ctgttgaggg agttgatccc
     7141 ctggccattt acggggtgaa caacacccgt ttcgagatga tttcccgcca ctaccccaag
     7201 ttaaaattag ttgccagggg taaccagatt aaggtaaccg gcgacgagga gctgattgat
     7261 gacctggaga gcaaaataaa ccgcatggtt gagttttaca gccgatactc ccggttaacg
     7321 gaggctaaca tacttgattt tctcaatgat aacgacagct caattgttaa ggccgaggag
     7381 gatgctctgg atgtgctggt ttatggcaca aacggccgaa ctgttaaggc gcgaaccatt
     7441 aaccagcgaa agctggtgga gctataccaa accaacgacc tgctttttgc cataggacct
     7501 gctggttcgg gcaaaaccta tacagcaata gctctggctg taagggcact ccgaaacaag
     7561 gaggtgaagc gaatagtgct tacccgtcct gccgtggagg ccggtgaaag gttgggcttt
     7621 cttcccgggg atatgaagga taagctcgac ccctaccttc aacctcttta cgatgcccta
     7681 cacgacatga taccacagaa aaaactgatg gagtatatgg acgatgggac tgttcagatt
     7741 gctcccttgg cctacatgcg cgggagaacc ctcgacaatg ccttcgttat actcgacgag
     7801 gcccagaaca ccacccttaa ccagctcaaa atgtttctta cccgaatggg taaaaatgcc
     7861 aagttcattg ttaccggtga cattacccag attgaccttc ccaacagagg tgattctgga
     7921 ttggtaaagg caattggaat tttggaaaac attggtagca ttgccatggt ttactttgac
     7981 gcaagggata ttgttcggca taagctggtg aagcacattg tgaatgcctt tgaaaggtac
     8041 acctccggta atgggagttc tgaaaggact gaaaataatg atgagtgtga ggataactct
     8101 cagtagtaac cttggttcta gtgaagtgcc atttgcaaca gcattatctt ctaaacacta
     8161 aacattgtgg gcgttggggt gtttgccacc aagaaacttt ttggtaatcc taatacactg
     8221 cactttaacc tcaatggaac agaagccaaa tcaactcaaa ctaaaataca gaagcaaatg
     8281 gaagcgattg taagaacaga tttcaaattc cctggccaaa cctctgtgta ccatggaaaa
     8341 gttcgtgatg tttacaatat tgccaacaag cacctgatta tggtggcaac cgataggata
     8401 tcggccttcg acgttatcct tcccaaaggc attccctaca aggggcaagt gcttaaccaa
     8461 atagctgcta agtttctgga tgccacagcc gacatagtgc ccaactggaa aatagccacc
     8521 cccgatccga tggtaaccgt tgggcgcctg tgtgaaccat ttaaggtgga gatggtggtg
     8581 aggggctacc tcaccggtca tgcctggcgg gagtataaat cgggtaaccg gttactttgt
     8641 ggggtgccac ttcccgaagg gatgcgcgag caccagaaac ttcccgctcc cataatcaca
     8701 ccaactacca aggccattga aggtcacgat gaggatattt cacgtgagga aattatcgcc
     8761 agcgggctgg ttgaagagca tgactacgca caacttgagc gctatacact cgctcttttt
     8821 gaacgcggca cccgaatggc cgaaaagatg gggctaattc ttgttgacac caagtatgag
     8881 tttggacgtg ccgacggccg catctacctg attgatgaaa ttcatacacc cgattcttcc
     8941 cgttattttt acgccgatgg ctataccgaa aggttcaacc ggggtgagga gcaacgtcag
     9001 ctttctaaag aatttgttcg cgaatggctt atggccaacg gctttcaggg acaggaggga
     9061 caaaaggttc cggaaatgac cccagaattt gtaaaccagg tttcggagcg ctacatagag
     9121 ctctacgagc aaataaccgg cgagaaattc cagagggcag agggctctga tgtaatgaag
     9181 cgtatcgagc gcaacatact caactttctt aaaaccctgg tttagcacca actcctgctc
     9241 cagtagattt ttctgaaacc atgttaacct tcaacccctt tgctgaatag ctttagcttg
     9301 gggttttata cctattccag cagttcaaga atggcttccc tgctaatctg gcctagcgaa
     9361 cttgacgagc ttacgaacag ctgggcaagg ttctcctttc gttcctgcat ggagagaatt
     9421 ttttcctcca cagtttcgga tgtaataaaa cggtaaacaa aaactttttt atcctgacca
     9481 atcctgtgcg accgcgatgc agcctgcatt tcggcagcag gattccacca tgggtcgaga
     9541 atgaaaacat agtcggctgc ggtaatattc aggccaactc cacccgcttt aagcgatatg
     9601 aggaatacct ttttatccgg gtcggtttgg aacctttcca caatttcgct ccggttggct
     9661 gatgaccctg ttaatttctc gtatcctatt tttctatcct gaaggaaaac ctctacctgc
     9721 tcaagatgtc tgacaaatga ggagaacacc aggattttgt gatgacctgc ggtggcgctc
     9781 tcgatgccca tagccacctc gtcaaacttg ccggaatcgg ccagccggta ttcgggcaac
     9841 atggccggat ggttggcaat ttgtctgagt cgggttaggc tagccagaat ggaaactgtt
     9901 gactgattag gggctggact gtctaaagtt tcgagtatga agttgcggac aaccgatttt
     9961 tccttctcgt aaatctcctt ttgtgaatcg gacattctgc agtaaaccac ctgctcgcta
    10021 atttcaggta ggtctttagc cacctcttcc ttagtgcggc gcaaaatgag aggctcaata
    10081 agctgcttca gctgcttttc cttttcgtga tctccctgcc gctcaatggg agtttgaaac
    10141 tcattgcgga aggcccttaa gccgcccagc attcccctgt tcaggaaatt aagctgcgac
    10201 cacaggtcga gcagggagtt ttcaacaggt gttccgctta ggctgagata gttgatggcc
    10261 ctcagctgca ttacggagcg gtagcctttg gtcaacggat tttttacggt ttggctctca
    10321 tccagaacaa catatcgaaa ggttacctca cggaacatgt caatatcgtt acggatgagg
    10381 ccgtacgtgg taaccacaac gtgatggttc agcaaccagc tggccgattt taccctttgc
    10441 tgccccatgt aggcggcaac tttcagctcg ggggtaaacc tgcccgcctc gctaatccaa
    10501 tttgccacca gtgatgttgg cacaacaacc aacgaggttt tacccattcc tgtggcataa
    10561 ttttgctcct cattgattga ttgtagcaac gttaaggctt gtatggtttt gcccagtccc
    10621 atgtcatcgg ccagacaacc gttgatgcca ttttggttta gcagcttaag ccatgcatac
    10681 cctacaagct ggtagcttcg tagccgggca tttagccgtt ggggtagctg gataccttct
    10741 tttttaaggt tggaaagcct gtgcctgagc tccgaagctg cgggggagtt gacttcattg
    10801 gtgtcggcta gcaggccaaa aagcgatttt ggcaacctta tgccttcatt ttcctccttg
    10861 cctatcagca ttaagggttt gtaacggctg aaccatacct ccggtataat gaaaacctgc
    10921 ccgttgggca gcagaaattc cctgatgttt ttgaggatgt tccgcttcag gtaaacaaat
    10981 ggaatttcaa acccatcgag cttgattttg ccatacaggt caaaccagtc ggtggtggag
    11041 tcggttgaaa tggccactac aaccccgttg aggtagaagt tcatacccct aaccagttgg
    11101 gcaacggcaa aaccttccgt tttaagctgg ttaacatgtt ggttgagcca ctccaccaag
    11161 gggtatagat tttcctttgc attctcaacc gctgccggta aaaattcggc ctcgctaacc
    11221 cgctgaaggt taaacttttc ggaaagtaag caaataactc tgctttccca tattctattt
    11281 cggttaaagc ggcgaaaggt gtacccgttg ccctcttcaa ccagcctgac ttcggtgttg
    11341 taaggggtat tgcacaggta tcccctgtcg ccatatgaaa attttagcgt taggcaccat
    11401 cctcccgcca acctctcctc aagggtaagg attgcccttc catcatgctg ctcatcaaca
    11461 atattgaagc caaccgcctc cacttgctgg tggcgtatgg cattcagcac aaaagtttgc
    11521 atgtatttct tttcggccga ttttggaacc gtgatgtagg gtttggatag gaatggagta
    11581 agttttttac catcaattgc ctcaaattgg taaattgttc tgtcaattcg aattatgcag
    11641 ggattgttgg taaccacctc gccgttggta gttgtcaggt ttactgttct gccgttgttc
    11701 atggccatga tggaatacat tatcgaatca ggggtgagtt caaatctgaa caggggcaag
    11761 gttggctcga gttcaacatt cagacggttt tcctcgtaaa gatttttttc ttcctttttt
    11821 cgcagaaaaa taggagtgtc ggagtgcgct gccagcgcaa caaccttggc aattctgcgt
    11881 tcaacgtagg agtgaacatg actgtttagc tgttcacttc gggtcaggga gtccagaaat
    11941 tctgccaccg attttgcctg tttgtcaaag tacttctgca gggccttgtc ggaaatttgc
    12001 tgcagctgct gaagcatatc cttctgaatg ggtgatacaa tgtcttcaag gtctgatggt
    12061 tgggctaccg ctgcattttc agaaatgatg taaataccat ggctttcggt gggtgttgcg
    12121 aggtaggctc caatggttgt tccaagctgt ctatggtgct gaagggtaag cgctaaaaat
    12181 ggtttgaggg tggccggcat agctacatta atttttttgc aattttagca taaatcagcc
    12241 aaacctttag tttcaaactt ataaggacta agagtaaagt taaaggagaa gggataaaaa
    12301 aacttggcca gcttcgatgg aacctgtccg gctttgacga attaaatgcg ataaaggata
    12361 aagaattgag agcagataag gaagaaatga ataaatactt atttagctgc ttgatattta
    12421 cttgttatcg gttcgcacat cacggtaaag gaatgacctg tgttaaattt ttagaattga
    12481 aaaccctcaa cccctatagc ttttccaatt attaaagtct tatttttgta gaatatattt
    12541 tgaaatttaa acgttattga aactctttga attcctagga taatttgcat agctcatatt
    12601 gaattttggc tactcctttt tataaaattc cttactattg aggattacgt taagtctaaa
    12661 acaatcggat tttaggaggt cataaaaagg aatgcatttt gttattgaag gtggcacagg
    12721 agtgtgtgca ttctgcctta tatttcttag gtgtggagga taaccttctt gctaggtaga
    12781 tgtcagattg attggtaaca ggaaaataat atcaacataa atatttttta gctgatgaga
    12841 tcctacatta cggccttgct tttatgccta tcacccatgc ttcttgccgg gcaggtagtg
    12901 gagggcgata taccctccgt tcaacgttct acagacaagg taaaaattga cgggaagaac
    12961 tactacatcc atatagttaa aagtggcgaa accctgtact ccattagcaa agcctatgag
    13021 gtttcacagt cggatattgc tgtcaacaac cccgatattt acaacgggtt gaagattgga
    13081 caagcgttaa agattccggt aagagcctct gaaagtaaag gaaccgatga ttacatatac
    13141 catattgttg ccaaggggga aacgctgtat agcatttcca gaaggtacaa catccctgtt
    13201 gatgtgctca caagcattaa ccccgaggta aaggatggtc tgctggttag ccaggttatt
    13261 cttattcctc gcaacccaat gatacccgag gcaaagaagc cggctgccga caccgtgcag
    13321 tttatcatgc acgaagtgca gaagggagag gggttgctgg ccgttgcacg acgatataac
    13381 atcgatgcta aaaccattga gacttacaat caggaagttc taaaggacgg ccttaagcta
    13441 ggcgagtacc ttagaatacc tgctgtaaag cagcaccctg ccgattcgct tatggttaca
    13501 agcaaaccag aaccggtggc attcttttct acctgcaaaa acaactttag gtacaacggc
    13561 caaccctttg acatttcgct acttcttccc tttagccagg gtagcgccgt cgatagcgac
    13621 gagcttatcg atgaggccga agaggtcaaa atggatcctg gtactaccaa cacccttgaa
    13681 tcgtcaagaa ttacctcgca ggaaactctt gcctccttaa acttttatga gggctttcta
    13741 cttgccgttg actcgatgaa gaaagctgga gtttccatga atatcaacgt tttcgacact
    13801 aaacaccaac ccgaagaggt tacaagtatt ctaaataagg gcaaactcga taagtccagc
    13861 attatcatag gtccactgtt tgttaacgaa atcaaaccag ttgtggctta tgccgaaaga
    13921 aaatcgattc ccatggtttc gcctctctac agtgggaatg aagttatatc agaataccct
    13981 ggtctaatat cggtgaacca aggatatggc gaacagctgg aggtttttat aaaaaacttt
    14041 aagtttagcg atacctgcca ctatgttgtt atttacgaca tggagaaact tcttacaggt
    14101 ccaattcaaa attttagctc tgcactcaac caaagaatcc gcgaatcggg tgttagggtt
    14161 gactctgttg ttcatcaaac agcagcgatg aaatcggaag aggttcaagc taagcttcat
    14221 agctacatga aagccgacgg gtgtaatgta gttataatcc cctcgcagga tgagcctttt
    14281 gtttccgatt tgctggggca actctatgcg gttaagtctt tgaatgggct taagatgatt
    14341 gtttatggcc ctgcacgctg gcaaaagatg aaaaatgttc cacctgatta cctgtacaaa
    14401 ctgaatgccc gtgtgtttac tccattttac gttgactatt caagagacga ggttaaacga
    14461 tttgttgcca gcttcaggga taggtacaga gctgaaccca accagttttc attcttgggt
    14521 tatgatgtgg gcatttactt tatttcggcg ctgaaaacct atggccccga ctttactaaa
    14581 tgtttaaaag atttccaaaa ggacatgctt cagtccacct ttactttcga tgtggtttcg
    14641 aatgaatcca gacttagcaa cacccaccaa tttattattg gctacaccga tgattacgac
    14701 cttgtgagga ttataccttg aaatggcctg cagcttcatt gcgaacgaat gagcgcatcc
    14761 agcttcagaa taactttttg gttgtgtaaa gaatagccgg aacattcgtt ttactccagc
    14821 atgttgttga taggttggag gttagatatt ttcctgtatt ttcctcctag cgggaacctt
    14881 tccctaagcg tgtctggtgg ttggggcagc tggcaatttt actgcctaaa aagatgccta
    14941 aaaatggtat tgttaaacat cttttgttta aggggttaca ttttggccat tcgtaattat
    15001 attgtatgtt tggttcaaaa ttaaactata aaaatccatt aaccatggct aatctggact
    15061 tatcggcata cggtgtaaac caacctgccg aagtttttta caatccgagt tacgagcttc
    15121 tttatcaaca cgaaaccgat ccttcactcc aaggatacga gagaggcatt gtaaccaagt
    15181 tgggcgctgt taatgtggac accggcgttt ttaccgggcg ctcacctaag gataagtatg
    15241 tggtgaagga gcccaatacc gaaaaaaatg tttggtgggc ccagccaggg aagaaaagtt
    15301 ccgacaacaa gcccatcacc caggaggtat ggaatcacct gaaggatatc agcgcaaaac
    15361 agcttagtgg gaaaaaactc tacgttgttg atgcctatag cggtgcaaat gaggacacac
    15421 gcctttgcgt caggtttgtc atggaggttg cctggcaggc acattttgta aagaacatgt
    15481 tcattaggcc aaccgacgag gaacttaaaa attttaaacc cgactttatt gttctggtag
    15541 cctccaaaac ctcaaaccct aaatggaagg agcagaacct taacagcgag gtttttgtag
    15601 cctttcatct taccgagcgc atggccctaa ttggtggcac ctggtatggc ggagagttga
    15661 aaaagggtat tttttccgta atgaactatt tcctgccctt aaaggggatt gcctccatgc
    15721 actgcagcgc taacgttgat caaaaaaatg ggaatgttgc cattttcttt gggctttcag
    15781 gaacaggaaa aacaaccctt tccgccgatc cccaacgcgc ccttataggc gatgacgagc
    15841 atgggtggga cgacaacgga attttcaacc ttgagggtgg atgctatgcc aaatgcatag
    15901 acctggaccc cgaaaaggaa cccgacatct atgccgctat tcgtcgggat gcactgctcg
    15961 agaacttgga tgttgacagc gagggaaata ttgactttca ctctaaagtt aaaactgaaa
    16021 acacccgggt atcctatcca atttaccata tccaaaatat tgttaaaccg gtttcgaagg
    16081 caggacacgc taaacacgta atattcctca ccgccgatgc ctttggagtg ctaccgccgg
    16141 tatcgcggct ttcgcccgag cagatgcagt accactttct aagtggtttt actgccaagc
    16201 ttgcaggcac agagagggga gtggttgagc cccagccaac tttcagcagc tgttttggcc
    16261 aggccttcct tatgctccat cccacggtat atgcccgtga gctggttaaa aaaatgcagc
    16321 ttcacggctc aaaagcttat ctggtcaaca ccggctggat tggcggaccc tacggggttg
    16381 gtaaacgtat tgacctgcct tcaacccggg ccataatcaa cgctatcctg aatggctcca
    16441 ttgaggagtc cgagtttgag ctgctacctg tttttaacct tcaagtccca ttaagggtta
    16501 acggggttaa cccacattta ttaaatcccc agaagtcgtg ggcaaatcca ggcgattggg
    16561 ataaagcagc cagaaatttg gctggaatgt tcattaaaaa ttttgaaaat tttaccgata
    16621 acgagtgggg aaaaagcctg gttaaagcgg gtcctcacat ctaaacttac taagtaagtt
    16681 tcggtttata cactacttgc tgcctaactc caccacatga ctgattatgt atgggcttgt
    16741 ggtggtttta tttgacacgg gttttaatac tcctggctag caataagcta tgcctcaggt
    16801 ttctaataat caacagcctt aaggtaaatc tcctatggct tgtattgaag atgtcctaat
    16861 tttggtaatt tgtgtgagcg attagcctag ctcaaccctg ggcgttttat aacatttttt
    16921 aacacttccc ccttgctttc aaatagctga catgaccagc tttaacctat aattttttcc
    16981 atgaatgttt cggtttaaag gtggtatgag taacttttaa cacatattct tttgccctct
    17041 taaagcaata ggcttatctt tgcctttatg gaaattttgt ccaaaatagc cattgtcttt
    17101 ctggcttacc tcatgggctc cattcctacc tccgtgtggg tgggaaaaat tttttatggg
    17161 gttgacgttc gtgagcatgg cagcggcaat gctggggcga ctaatacaat acgggtgctt
    17221 ggccttctgc ctggaatcgt tgtttttatt gttgatgttt taaagggtta ccttgctgtt
    17281 aggctgcttt acctcaccga tttttacata cctaaaacag gcatgttcat taatttccag
    17341 ctcattcttg gaattgccgc catgattgga catattttcc caatttttgc ccagtttcga
    17401 ggtggaaagg gggtagccgt tctttcgggg gttgtttttg ccctgcatcc ctatgccact
    17461 ctgattgttt tcgggatatg gattgtatcg gttatcatta ccggctacgt ttcgctaagc
    17521 tccatgattg ctggtttttc atttccactt attttgatat ttgtttaccg caccacaaat
    17581 ccctccctga tacttttcgc ctttattctg gccatcctta tgctattcac ccatcaaaaa
    17641 aatattgaaa ggctgtttaa gggggaggaa tcaaaatttc acttgaaaaa gaaaccacat
    17701 aacgagcccg ataagcctaa atagccagtt tatgaattac tttatcggct ttatttgacc
    17761 taacctgaat agccctataa ggcaaatgca aattttttac gccccaaata tcacgggaag
    17821 cagctatacc ctgcctgaag atgaggcacg acactgccta agggttctta ggatgagggt
    17881 gggcgacgag gttgagctgg ttgacgggaa aggaaatctt tttaccggca ggatagccaa
    17941 tcccgaccta aaggagtgta ctctggaaat tgttcggaaa gccagcaatt tcggtgagcg
    18001 ccacttctac cttcacatcg caatagctcc aactaagaat gccgatcgtt ttgaatggtt
    18061 tgccgagaag gttacggaaa ttggagttga tgagatcaca cctcttcttt gcgagcgttc
    18121 ggagcgcaaa tcggtaaatt ctgaaagaat tgagagaatt gtgatttcgg ccatgaagca
    18181 atccgaaaaa gcctatctac ccacactgaa tacaatgact agcctggaga agttggtggc
    18241 aagcactcct gttgattttg ttaagctaat cgcccactgc aacgagaagg gcataccgca
    18301 tcttaaaaaa atggtaaaac caggcgataa agtgctcatt ctcattggtc ccgaaggcga
    18361 tttttctcct aaggaggtgg attttgcact cataaatggt tttagagcaa tcagccttgg
    18421 caattcccgg ctaagaaccg aaacggctgg tatcgtcgct tgtcatgcgg taaacatgct
    18481 aaacactgaa gatttttcat aaatttacca ccatcttcta aataaaattg gcttgagaag
    18541 actactagtg ggcgggtacc gattttcatg gttccacatt gctgcagcat tgctgggagg
    18601 agtggtgcta ctttttattg ttgccccaat tttggggatg attatttcca cccccgccaa
    18661 gagcttgatt gaaacagcag ctgagcagga ggtggtgagg agtatatggc tttcggtttg
    18721 ggtagcaatg gcggctacgc tggtatttgc ccttttctgc ataccacttg cctacttgct
    18781 ggcgaggttt gactttccgt tcaaaaggtt agtattggga attatcgatt tacccattgt
    18841 tataccccat tctgcagctg gtattgccgt acttgggctt attgcccgtg atggtctctt
    18901 gggacgatta acttctccgc tgggcattga gctggtgggc tcaccggctg gtattgcaat
    18961 tgccatggca tttgtgagcg tgccattttt gattaatgcc gcacgcgagg gttttgccct
    19021 tgttcccgag cgtctcgaaa aggctgccct aagccttggc gcttctccaa ttacgatttt
    19081 ttttactgtg gcgcttcccc ttgcatggcg tagcgtggtg tccggactag ttatgatgtt
    19141 tgcacgcggt atgagcgagt ttggcgccgt ggtgattgta gcttatcatc ccatggtggc
    19201 tcccgtttta attttcgaac ggtttggtgc ttttgggctt aagtatgcca agccggttgc
    19261 cgttttgttt ataggtgtta gccttacggt gtttatcatc atgcgacttc tttcacgtaa
    19321 caggtcgaac catgcttagg gttgaagggg tttgcaaaaa gtttcaggat tttagcttga
    19381 accagatttc gttcacggtt gaccaagggg actacttcat gctgttgggg ccatcggggg
    19441 caggaaaatc gttaattctc gaaaccattg ctggactgca caaaccagac tccggaaaaa
    19501 tattttttca gggtgtcgat attacccata ttggggttag ggagagaaat tttggaattg
    19561 tgtatcagga ttatgccgtt tttccccacc ttacagttta cggcaacatt gcctatccgc
    19621 ttcgccggaa aggtataagg ggtaaggagt tgcataagca ggtaacaagg tttgccgaaa
    19681 gggtttcggt agcgcatttg ctagccaggc atcctcaaac tctttcggga ggggaggtgc
    19741 agcgaactgc tctggctcgt acattggcca tggagccacg tatcctgctt ctcgacgaac
    19801 ctctttcgtc tgttgatgtt aagcttcggt ctgagctacg ctccctgctt agaagcataa
    19861 atagcagcgg ccaaacggtg attcacgtta cccatgacta cgaggaaacc cttgcgcttg
    19921 catcccacct ggcagtagtt aacgggggtt gcattgagca ggttggaagt gttcaggaga
    19981 tttttcagaa tcctcgctcg gagtttgctg ccatgtttgc cggagttcat aacttttacc
    20041 ccgctagtat tgtagaattt tcggagcatg gattaacaat ggtcaggttg aacgacacta
    20101 ttgctatatg ctgctacacc gaaggttgca atggagaggg ctttgtttgc ttttccgagg
    20161 agtccgttat cctctcaacc gagaaaccgc atcagagtgc tcttaatgtt tttcggggta
    20221 gagtagtgga aattttcccc cagcgttttg gcctaatggc tgttgttgat gctgggttta
    20281 gggtgctttc gaaagtaacc tgggaatcgg caagccgttt cggtcttacc cccggtgttg
    20341 aggtgtgggt atcgttcaag gcaagctcag taaggtttat ccctagggcc agctaattta
    20401 cctgggcctg ctatatgatg aaattttcct gctattctgg taaaccattg aaacatactg
    20461 aatgccaaaa aatatcaaca ggaatacgat ggcgtagtag tagagcgaaa gtgtgaggaa
    20521 aaaagtcgct gcaaaaataa aaacgctcca gagcaaatca acaaaagtaa gaaccactcc
    20581 ccatagaatc ccgcttttaa taaccgttcc gagtaaatca accaaaatgc ttaccagcaa
    20641 tgatagggct tgacaccaaa ctacccaatc tattatgtcg ggattgtttg gaatagccct
    20701 aatgccatgt tctatgctgt taagtagtaa atccttccag ttgatgtata gaattgccag
    20761 gagcaatgcg ttgataaaaa aaaccaccgg cattagtaca agccccttta cctgaaaatc
    20821 atgtttttgg gccagtcggt ttttatactg aacgattagc ttgcctgaaa aaatccaaat
    20881 cacgattgca ggaatagcta ttagccagaa cacccacgat tcccagctgg ctgttttctc
    20941 aacccagctt aggagattcg gagttggtgt gggttcaatc cggttgaggt aggaagctgc
    21001 aacatatccc tttttagtgc caaccttaac cgtcacccat tcggtatccg agacaatagc
    21061 atttatcgta tcgccctgtt gaagggtgac aactggggca gaagcggttg agggggagct
    21121 gcgaacaatt aacgttttgg cctttaccac gtagcgttct cgaatgttat agctcccggg
    21181 tttcaggcat gagacaacca ccaatggtaa caggaggaaa ataatgcacc taaccccact
    21241 ttgcctcata gtatagaaag ttgaatccag cacaaaggta atgtctgaag caaatgcagc
    21301 aaaaaaatta gtttaaaaaa taaacctaat ggttaaaggg gaagcggttc tactgtttgc
    21361 tagtcatttc attaaaagct aaacaccctt cagaaggtaa caggataagt tcttcttttt
    21421 tcttaaagct gaaagctatg cttagtctat gttaacacct aagtaaaatt aagagtctaa
    21481 aaatcttact ccaggaattc taatgttttg gccaaagcgg catccagacc tgccgggttt
    21541 ttccctccgg ctgttgcaaa aaagggttgg ccaccaccgc ccccttgaat ttccttagcc
    21601 gcctcacgaa taatgcttgt tgcgtttagc tgcaaagatt ggacaaggtt atcgcttatc
    21661 ataactgtta gaattgcctt ttcgcttgcc gttgccccga tagccaggaa aaggtttgga
    21721 acttcattcc ttagctgaaa ggctaaatcc ttgatggtag ttgaatcgtt caggcctacc
    21781 ttttggcaaa taacgttgat tccgtttcgc tgttcaatac tttgcttaag ctggtgctta
    21841 acttcggcca acttttcagc ctgcaggctt gaaatctctt ttcgtagcat ttcgttatcg
    21901 gctaccatac gggcaacggt ttgggataca ttggaggggt ttcccaacaa ggacttaatc
    21961 tccttaatgg tatcctcctg agagtaaaca aactcctccg cttttatccc ggtaatagct
    22021 tcaattcgcc ttactcctgc agcaatagcg gtttcggata aaatcttgaa aaatcctatt
    22081 ctacctgttg cttcaacatg tgtgccgccg cacagctcaa cggactcgcc aaaatggata
    22141 accctaaccc ggtcgccata cttctcgcca aaaagcgcca tggcacccat acttcgggct
    22201 tcgtctatag gcatgttcct tttctcatca agatgaacat tttcacgaat aacgctgttc
    22261 accagtcttt caacctgtcg gatttcgtcc tcggacattt tctggaagtg cgaaaagtcg
    22321 aaacgtaggt agtcgggatg aaccagcgag cccttttgct caacatgtaa tcccagtatt
    22381 tttcgtaggg cgttgtgaag caagtgtgta gctgagtggt tggctgctgt gcttgcccgt
    22441 cggttgacat caaccgatgc cacgaatgtg ccgttagggt tggccggcag cttgtcaaca
    22501 atatgtatcg tcaggttgtt ttccttaatt gtgttcaaca ccctgatagc ttcgccctcg
    22561 ccggttatgg ttcccgagtc gcctacctgt ccgcccgatt cggcgtagaa tggggtctta
    22621 tccagcacca gctggtactg ttcgtttgac tttaccctta cggtgcgcat cctggcaata
    22681 tgtacgttac aggtgagcgt atcgtagcct acaaactccg cattttcgtc atcgttcact
    22741 acaatccaat ccatttcctc taccgcggca tcggctctgg agcgttcctt ctgcagcgcc
    22801 atttcgccat caaacccgga atgatcgacc gacaggccat tttcccttag tattagctcg
    22861 gttaagtcga gtggaaatcc ataggtgtca tagagaacga aggcgtcttt tcctgaaatt
    22921 tccattttgt gctctttacg ggcgcgctca accagctggt cgagcatgcg cattccggtt
    22981 tcaagtgttc tgaggaaagc tgtttcctcg ctggtaatga cacttgtaat aagcgattgc
    23041 tgtttcctaa gttcaggata ctggtcgccc atctgctcgg ttagtaccgg aataatacct
    23101 gccatgaaag gctccctgaa gttgagaaat gtgtagccat atcgaacagc ccgtcttagt
    23161 atgcggcgaa tcacgtagcc tgccttaaca ttcgatggga gttgaccatc ggctatggca
    23221 aagcagatag ctcttaggtg gtcggcaatt acccgcatgg ccacgtcgct tttggcatca
    23281 gcaccatact ttaggccgct tatgttcgat atgcggtcta tgagcggttt gaaaacatcc
    23341 gtatcgtagt tgctcttttt cccttgaacc accattgata ggcgttcaaa acccataccg
    23401 gtatctacgt gctttgcggg taggggttca agctggccat tagccttgcg gttaaactgt
    23461 ataaaaacca ggttccatat ctcaattacc agcgggtggc cggtattcac catcttttcc
    23521 ccggggttag ctttccgctc atcttcacca cgcaggtcta tatggatttc ggtgcaggga
    23581 ccacagggtc cggtttcgcc catttcccag aagttatcct tcttgttgcc ctcaataatt
    23641 cgattagagg gtaggtgttt tgcccagcag tttaacgcct catagtcctt ttcaacaccc
    23701 tcatcggggc ttccttcgaa aattgtagca tacaacctgg tgctgtcaat tttaagtaca
    23761 tctaccagaa attcccatgc ccagtcaatg gcttcgttct tgaagtagtc gccaaacgac
    23821 cagtttccca acatttcgaa catggtgtgg tggtatgtat catgaccgac ctcttccaag
    23881 tcgttatgtt ttcctgaaac cctaaggcat ttctgcgagt tggccactct tttgaatttt
    23941 gccggagtat tgcccagaaa aatatcctta aactggttca ttccggcgtt ggtgaacata
    24001 agggttggat cgtttttcac caccattggt gcagaggcaa caatggtgtg cgatttgcct
    24061 tcaaaaaagt ttaaaaatgc ccgacgaatt tctgcagaag tcataaaatg cttgctttga
    24121 aaaggttaac atttattaat taatcatttt tcaaaaaaaa aattattttt ggcattgaaa
    24181 aattcatttt ttaccataga tttgttgcat cttatcaaaa aggaggcaaa catagttaca
    24241 aaagttaaaa aaggatggta aaagtaaaat atcatttcaa ccctcatacc cttaaatttg
    24301 atgctgtttc cctgcctttt tataagcggt tggcaaagat actaattcat ttgggatttt
    24361 accttttagt ttttgttgtt ttggggtatg ggttttccat ggtgtttgat actccaatag
    24421 cgcagggttt gaagcgcacc aattctgagt actcgctaaa gtatgaaatg gccttgcggc
    24481 agatgcagga gttcaatgac atacttactg aaattgaaca gaaggataac aacatctacc
    24541 gggcaatttt tgaatgcgac tccattccgt tctccattag gaggggtggt tacgggggtt
    24601 cggaaaggta ttccgagctg gtaaacaaca gtaactcgca tttgctggtg aagactttta
    24661 aaattcttga tgagctgtca tggcgggcct acatccagtc ccgttcactc gacgaggtta
    24721 ccgagttggc catcaataaa gagcgaatga ttgagtgcgt tcctgccatt cagcccattt
    24781 ctgttaaaaa tctggttaga atatccgact tttttggcta taggagagac ccaattaccc
    24841 gtgctccaac tatgcacgag ggtttggact ttgctgggcc tattggaact cccatttact
    24901 caaccggcga tggtattgtt attgaggccg gctactcctt taatggctac ggtaaccagg
    24961 ttatgatcga tcatgggttt ggctataaaa cccgctacgc acaccttagc aaggtaaacg
    25021 ttaaggtggg tgaaagggtt aaacgggcgg aggtgatagg caagcttggc aataccggga
    25081 agagcaccgg tcctcattta cactacgagg tgattgttcg gggtaaaccc gttaacccga
    25141 taaactactt taacgatatg accgagcagg attacgaggc catgctcaag aattattcta
    25201 gccaattcct tgattaacta tctacattgg aaaaatggca aagtacaagt ataaattcca
    25261 tcccgagcag ctaacattcg ttagggttag agctaccctt aaagaaaagt tgtttgttgc
    25321 cttgaagtac atggtggcaa cagctattgt tggtataata tcctacctgg ttttcccttt
    25381 ctttatcgat acccccgagg ttcgaaagtt aaagcgggag aaccaggagc ttatgcttaa
    25441 ctttgaactt atcgacaagc ggttggatca gctcagagcg gttattgaaa acctacagca
    25501 gcgcgacgat aacatttacc gcatcatttt cgaggccgaa cctattcatc cttctgtaag
    25561 agaagcgggg atgggcggtg ttgacaggta ctcctacctc gaaggccttt caaacaacaa
    25621 gattattgtt gaaactgccc ggaaaattga ccttttaagc aaaaaggctt acattcaaag
    25681 ccgttcgttc gatgagattt ccactctggc taaaagaaag gaggaaatgg tacgctgcat
    25741 tcctgccatt atgcctataa acaataaaga cctcacccgg atggcctcat cgtttggcat
    25801 gagaattcat ccattctaca aggtgttgaa aatgcacacg ggcatggatt ttacatcccc
    25861 gttaggcacc gaggtttact caacaggcga tggggttgtt accaatgtgg agtatgccca
    25921 acggggctat ggctacaaca ttattgttga ccacggattt ggctacaaaa cccgctacgc
    25981 ccatttgggg aaaattattg ttaaacccgg ccaaaaagtt aggcggggca tggttattgg
    26041 cctggtagga aacacgggaa cttctgttgc gccccatctt cactacgagg ttatccgtaa
    26101 caacacgccg gttaacccaa tcaactacta cttcaacgac cttacacccg agcagtacga
    26161 taagctggtt gaaatagcat ctcagccaac ccaaagcttt gactaaacga ttcttttgaa
    26221 ctaatgcctt acaaggaaaa aaatatcgaa aaattctact tttccattgg agaggtagcc
    26281 gaaatgctcg gcgttaacgc ttcccttatc aggttctggg aaaagaactt cgacattatt
    26341 cagccccgta agaatcagaa gggtacgaga ttctacacca aggaagatat tgacaacctt
    26401 aagctaattt accatctggt aaaggagcaa aggatgacca ttgagggggc gaggaaaaaa
    26461 attgccgagg acaaacattc cgtatccgat agctttaaga ttatcgaaag cctgaactcc
    26521 atcaagcaaa tgctactgga agttcgcgac cagctcgatt agccaaattc ttcaagtctt
    26581 caaacccaaa tgaaactcga cgaaattgtt tcgtattttg aagagcttgc cccaacagcc
    26641 taccaggagg aatacgacaa cgctggtctt attgtaggcg atgcctcaat ggaagtaaag
    26701 gcagctcttc tcactcttga tgttaccccc gaggttgttc ttgaggccat tgataaaggg
    26761 gctaacctga taatctcaca tcatcctatt atttttaggg gtattaaaaa gtttaccggc
    26821 agcagctatg ttgaaaaaac agtagcagca gccatcaaga acgatattgc catttttgca
    26881 gcccacacca acttcgacag catgatgggt ggggttaact tgtcaattgc ccgtaagttg
    26941 ggtattacca atctggagat tttgcagccc ataaacaatg ggcttttcaa gctggtaacc
    27001 tttgtccctg tcgatagcgt ggaaaaggtt agagatgcca tgtttaaggc cggagccggt
    27061 caaattggag gttacgattg ctgttccttc ggctccgagg gctttgggac attccgcgct
    27121 ggcgacaaca ctcaccccta cgtgggccaa aagggcgaga tgcacaggga gcccgaggtt
    27181 agggtggaaa ccattcttcc aaaaaatatc cttgcaagag tggtggaaaa gatgattgaa
    27241 gcccacccat acgaggaggt ggcatacgat atttaccctc tcgaaattcc atacaggcag
    27301 gttggattgg gaatggtggg gaatattgag gagcctgttg cttcagttga ctttttaaga
    27361 caggtaaagg aagtgtttgg cgctggtgtg gttcgctaca ccgcgctttc acgtcaagtt
    27421 atcaaaaggg ttgcattctg cggaggttca ggtgccagcc tgattggaaa ggctattggg
    27481 tcgggcgccg acatatttat tacctcagat atcaagtacc accaattctt cgatgccgat
    27541 ggcagaatta tcattgccga cattgggcac ttcgagagcg aacagtttac tattgaatta
    27601 ttttatgagt accttttgaa aaaattccct acctttgcgg tcttaaaatc aagggtaaat
    27661 accaatccaa ttaactactt atagcttgta aaatatggca gcagtgaaga acaaaccagc
    27721 tgaacctacc gccgagcttt cggtggagga gaaattacgt tttctgtaca tgcttcagca
    27781 ggttgattct aagattgacc ggattagaat gctgagggga gaacttcccc ttgaggttca
    27841 ggacctggag gatgaaattg aagggctcga aaccagaatt gataacctga aacaggaaat
    27901 caagagcctt gagagcgagg tgtctgccaa gaaacaggaa attaaggatg ccaatacgct
    27961 tataaaaaaa tatcaggagc agcaaaaaac tgttaggaac aaccgggagt ttgattcgct
    28021 ctcaaaggaa attgaatacc aaacattgga aattgaactt tgcgaaaagc gaattaagga
    28081 gtttacccaa caggtaaagg ataagactct tgttattgag cagtcgcaaa agttgcttga
    28141 ggagcgaaaa ctcgatctaa agggaaaaag aatggagctc gataatatta taaacgagac
    28201 ccagaaggag gaagaggagc ttattaggaa atccgaggag tatggaaaga ttattgaacc
    28261 caggctttac acagcatacc gtcggattag gggaaatgca cgcaacggct tggctgttgt
    28321 taccgtgcag cgtgatgcct gcgggggttg cttcaacaag attcctcccc agcgccagct
    28381 cgacataaag attcataaga aaattattgt gtgtgagtac tgtggccgaa ttctggtgga
    28441 cgactctttg gccagcatgt aatagaaaca accttcgtat gcctccaagc ggtttagtct
    28501 tgggggcttt tttattgctt actcctcgtc aaggctttta ttccttccta aaccctgaac
    28561 aaccactacc acttcaccct taacggtttt cgagttgaag tgtgctgcca cttcggctag
    28621 tgttccccgc attgtttcct catgcagctt ggtcagctcc ctcgaaacgc taaccctccg
    28681 gtcggggccg aaaacagcca tgagctgctc cagcagcttt accagccgaa agggcgattc
    28741 gtaaaacacc atcgtgcgat cctcgtcttg aagtgcctca attcgcttgc tgcgaccctt
    28801 tttctgtggc aggaaccctt caaacacaaa tctatcgcag ggcataccgc tctgaaccag
    28861 cgcaggaaca aaagctgttg ccccgggtag gcattccacc tcaactcccc gttccaggca
    28921 agtgcgcacg aggaggaaac ccggatcgga aatgccaggt gttccggcat ccgatatcag
    28981 ggcaacgtca agtccggcgg ctatctgttc ggcaattgct tcaaccgatt ggtgctcgtt
    29041 gaacttatga tgcgatctta gtggggtttc gatgctgtaa tgcttaagca gattcccact
    29101 ggtgcgggta tcctctgcaa gtactagcga aaccgaacgt agtacctcca gcgctcttaa
    29161 cgttatatca cctaaatttc ctattggcgt aggaacaatg aatagtttgg acatggattg
    29221 gaggttagcg tgttttttta ctttggtgaa gtttcaattt caaacatttt gggtgggtgg
    29281 ggctttacat ctgccagcgc tatcaaatgt taaaaaatga acattactaa aaaaagtaac
    29341 agggaagggc aggaggtagt tacagtgcaa acttcctgcg caataaatcg ataaattttg
    29401 aagcagcttc gctggttgca ctccagctaa agttatcctg aaggtaaata atggcctcgt
    29461 tcaagtcgtc catctcgtta agacggttta ccgtttcggc atagagcgcc gaatcgccat
    29521 caaacagctc gttggtgaac aggaacttat cgttaatgcc tattgccttg gcaaggtcgg
    29581 taatgggctt gttctgcagc ttggtgccca tatcggacct gcgcgcttgg ttggcaatag
    29641 tttcgttaag tgacttttgc ttgtcctgaa acttatcggc caacgttgcc ggtcgatcgg
    29701 tgctggctgc tccgggggtt ggctcaacct caatactttt actttcattt gtagatggag
    29761 ccttttccct gaacttttgt atttgctcgg gtggtttctt tgagtatgaa ccagctggag
    29821 caacaggttc aacattatgg cttggggtgg ataaaggctg atctcttttg atgccttgag
    29881 ttgtagtttc ggcatttgcc ttaacggtat gctggctatg ggcttgatgg gtaggttcag
    29941 cctgcttttc aacgctaaac aacagcagct cgtaggcttt ggcaagtttg tcaagcagta
    30001 tgttacgttc aattgacggc agattttcgg atgccagggc tctttcaatt attttcatgc
    30061 tgtcgtctaa aagtgttttc gagtctcgaa gatattgcat cactgtcaac tgttaagatt
    30121 tttcgacctt caaaaataag ttttattaaa tttgtatgta aaactaacgc taataattca
    30181 ttttagaccc atgcctttta tcgaaaattc tgtaagcaac aagcgcaatg gatggataga
    30241 ggttattgcc ggttcgatgt tttcgggtaa aacagaggag ctaatccgcc gactaaagag
    30301 agcaaagtat gccaggttgc gcgtggaaat tttcaagccc caaattgata cccgttactc
    30361 cgaggagaag gtggtatcgc acgatgccaa ctccattatc tccacgccgg ttacttcatc
    30421 gggcaacatt ttactgctgg cacaggatgt agatgtggta ggaatagatg aggcgcagtt
    30481 ttttgatatg ggactaccta gggtttgcac ccagcttgcc gacatggggg taagggtaat
    30541 tgtggcaggg cttgatatgg attttcgtgg taaacccttt ggcccaatgc caaacctgat
    30601 ggctattgcc gagtatgtta ccaaggttca tgccatctgt gttcgctgtg gcgagctggc
    30661 tcatttctcg caccgaactg ccgacaccga taaactagtg gtgctgggcg aaaccgacat
    30721 atacgaaccc ctttgcaggc actgctataa tgaagccttg agcaaaagca aaacctaaca
    30781 aatggcctat cgggaagtta gctttcgcat tagtcgtaag aacttctggt tcataacctt
    30841 tctcatagtc cttctaccca tttcttcaaa gtaccggttg ctcatctttg gtaaacgaac
    30901 gcaaggcgtg gtggttgcca acgaggtgaa gtcatctgct ttttatcagt cgcatggtta
    30961 cgactcctac ccggttattg agtttaatgc agattctcta acactaagaa tgtatggccc
    31021 cgagaatacc aactatgaag ttggacgtag gtttacagtt atttacaacc ccgacaatcc
    31081 atataactgt atgattttat cgctctccta cttttatacc gggcgtggcg ttattctgcc
    31141 cggaacaatt atgcttttat ggatttcatt ttacctgacc tttagaagca gggatagcaa
    31201 aaataaccct cgaaagggtg aaagggattc tccgtaggcg tagtgaaatt gagcattgta
    31261 gttgaaggtt atagtaagaa tgcagctttt cggattcact tccgggtgtt caagttacgc
    31321 ttgtttgagc ctggttttcc tgaatgattg gcagaaccac tatattccta tattttccct
    31381 gaaaggtttc gtatctggga gctagtaaaa attgttactt acacagaata tggtcgtaga
    31441 gcatgctctg aaattgggat tttatattga tataactcaa aaccaaacag aaaaattgca
    31501 aaattactag cggcatgatg tacgaattat tctcggaatt gtttaaaaac caattgtaga
    31561 atcatcatgc gtataggaga gttgccgaag tttaaagatg cgatagagga gttgaaaata
    31621 taactctctc tgtgctgctc catgctctct gtggtaagaa aagcccaacc acggaggaca
    31681 cagaggtaca cggagaaaaa a
//
LOCUS       DAOSHM010000028        31125 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_1129_length_31125_cov_19.931703, whole genome shotgun
            sequence.
ACCESSION   DAOSHM010000028 DAOSHM010000000
VERSION     DAOSHM010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 31125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31125
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1129_length_31125_cov_19.931703"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(529..2307)
                     /locus_tag="PK839_06005"
     CDS             complement(529..2307)
                     /locus_tag="PK839_06005"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOG72326.1"
                     /translation="MSESKLLKSGEFLVNEVEANDIFIPEEFNEEQRMIAQTCRDFLE
                     AEVYPKLDDLDKSDRELMKSLLKKTGELGLLGIAVPEEYGGFGQSFITQMLVAETTGA
                     GYSFSVAYMAHCGIGTMPILYYGNEEQRQKYVTRLATGELLGAYCLTEPGAGSDANSG
                     KTNAKLSEDGKHYILNGQKMWITNAGFADTQVVFAKIENDRVLSAFIVESKWPGVVVG
                     PDEHKMGIKGSSTAQIYYNDVKVPVENLLGNRGEGFRIALSILHMGRMKLGANVIGAA
                     KETITQSVRYANERKQFNTQIANFGSIKHKLAEQVIRTFAHESAIYRVSQNIDELITR
                     YKAEGCDYGKAAIDAISHYAVEDAILKVNGSEMLDFVVDEGVQIHGGMGYSAEMNVER
                     GYRDSRINRIFEGTNEINRLLVVDTAIKRSLKGDFNLMGPAEELYTNLDGIKVEMGAA
                     GYFEQKKQLIRNFKKIALLGIFGANKTFAKQFVSEQEVQNNLSNIIMDIYVAESLALR
                     VEKLESRGHASMDIYRSILDVFIFDAASRIRKNAIDAILSFAAEPELGKLIAATERLS
                     AVNPINVKEARRKIADKLIADNVYKF"
     gene            complement(2334..3128)
                     /locus_tag="PK839_06010"
     CDS             complement(2334..3128)
                     /locus_tag="PK839_06010"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOG72327.1"
                     /translation="METKKIEVPVMAELFARGEKPEYLFWVGCAGAYDDRYKKVTRAF
                     TKILVHLNISFAVLGKEETCTGDPARRAGNEMLFQMQAFQNIELFKSYDIKKIITICP
                     HCFNTFKNEYPDLGGSYEVISYTQLLEQYITEGKLKLDAGKFKNTRITYHDPCYLGRA
                     NGIYEEPRRIIKTLTGEFVEMERNRSFSLCCGAGGAQMFKEAEKGNKEVFIERTEDAL
                     KVNPEIIATACPFCMTMITDGIKYKNQEEKIRNLDIAELIAESLNL"
     gene            complement(3182..4501)
                     /locus_tag="PK839_06015"
     CDS             complement(3182..4501)
                     /locus_tag="PK839_06015"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOG72328.1"
                     /translation="MTKQVVFALALLVTLVAFGFTITRIIRFFSLTRKGFPVKNIGKR
                     IGIMLKVAFGQTKIFRRPIIGFLHAMVFWGFCLIIFGSIEMVIDGLFGLEKSLSVLGW
                     FHDFIMAAGDIFAPIIAIAIVVFLIRRVFLHIKRFEGIEMKKISHIDANIALTLILLL
                     MISLMGMNTAYVSWCHSLGKEIHGVYPVSQHLTGLFSSMPVEVIYTYYEVFWWSHILL
                     IFLFANILPYSKHFHVFMSIPNVFLSRLEPLGKLPSMESITREVKLMLDPNAAFAAPP
                     SDTPIERFGVKDAEDVVWKSYFDSLSCTECGRCTSVCPANLTGKRLSPRKVMMDLRAR
                     MKEKGPLMLKNGRDYSDGKSLIRDYISEEELWACTTCNACAQECPININHPTLIVDMR
                     RYLVMEEGSAPGEIKAMFNNIENNGAPWQYSPEDRLNWANNLEINIQ"
     gene            complement(4787..5752)
                     /locus_tag="PK839_06020"
     CDS             complement(4787..5752)
                     /locus_tag="PK839_06020"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HOG72329.1"
                     /translation="MSVLVYTENWDGKFKKLSFELVSYATRVAEMLNTSCIALSIGNV
                     DDAELKKLGSYGAQKILSVNSDNLKVFDTQNYTEAIAQVAKNNDAKVVVISNNNVGKA
                     VAPRLSVKLKAALAAGVNGLPLSTNPFTVRKRAFSGGAIAHVVLKTDVKIVTLPQNSV
                     DVTETPVNAEVEKISISLPEPKTVVKDVQKQAGKLLLTDAEIVVSGGRGMKSPDNWGP
                     LVELAELLGGATACSRPVSDEGWRPSEEHTGQTGKIIAPNLYIAVGISGATQHIAGVS
                     GSKYIVAINNDKSAPIFEVAQYGIVGDAQKVLPKLVEAVREIKGK"
     gene            complement(5778..6527)
                     /locus_tag="PK839_06025"
     CDS             complement(5778..6527)
                     /locus_tag="PK839_06025"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HOG72330.1"
                     /translation="MKILVCISNVPDTTTKVKFTEGDSKLDTNGIQWVINPWDELSLT
                     RALELKDDGNTGVKSVSVAHVGLSSSEPTIRKALAIGADDAIRVNADSSDAYFVAAQL
                     AEVVRKEQFDLILCGIESSDYNGSSVGGMLAEFLGIPSISAVSNLKIENGQVVITREV
                     DGGKEVVTTPTPLVAVVQKGIAKEPRIAAMRGIMTARTKPIKLYEPVPVEIMTEYVKY
                     ELPKPRAACKFVDAENPKQLIELLQTETKLI"
     gene            7024..7827
                     /locus_tag="PK839_06030"
     CDS             7024..7827
                     /locus_tag="PK839_06030"
                     /inference="COORDINATES: protein motif:HMM:NF022188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HOG72331.1"
                     /translation="MTKADSFNRDVVFIGAGNLATSLSVAFFNAGWRVVQVYSRTYQS
                     AATLANRLNAEPTATLNSLIRDAQLYVVALPDSVIQTVAEQLPSLKGFLVHTSGSTSI
                     EVLSVADVLGYGVFYPLQTFSKARLVEFKNVTVCIEGNSQATLNALSSIAQAITSSVV
                     ALDSEKRRWLHLAAVFASNFTNHMLAAAYRIAEQNGVDFKIFEPLVRETVSKAFLANP
                     KDFQTGPAVRFDQQTMEQHLSMLGFSDLELQNIYKELSLSIQNFAQSKK"
     gene            7833..8360
                     /locus_tag="PK839_06035"
     CDS             7833..8360
                     /locus_tag="PK839_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HOG72332.1"
                     /translation="MRKNFKEMLALVKAFVFDVDGVFTDGTIIIHPSGELLRTSNTRD
                     GYAVGVAVGRGFPVAIISGGKSDAVGVRFRGLGVQDIYLGVADKMIALEEFRKKYDLE
                     LANLLYMGDDLPDLEVMKLVGVPTCPHDAAYEVKQVAVYISSFNGGRGCVRDVIEQVL
                     RLNGKWGESSDMYMQ"
     gene            8454..9410
                     /locus_tag="PK839_06040"
     CDS             8454..9410
                     /locus_tag="PK839_06040"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglycerol-phosphate
                     geranylgeranyltransferase"
                     /protein_id="HOG72333.1"
                     /translation="MKFFQLIRYKNLLIIIATMILMRYFIIEPILLSYGLALQMPTGV
                     FIMLVMATVLIAAGGYVINDYFDTRTDLVNKPGEVIVGQEVTRREAIAMHMVLSTLGI
                     LLGLVVSFKVGRPVFSLLFFLTAGILWFYSTLYKRQLIVGNLVVSVLTGLIPLIPLVF
                     ELPLLGNFWEVIAVYQLDLHQLVYWVGGYATMAFLLTFVREIIKDIEDFEGDFTYGRN
                     TIPVRYGIKTAKVTAASMLGITMLALVYLFAAYMRQLSTGEFDFVTFSYFFLFIVVPC
                     FVLLVMILLAQEKKDYHRASGFCKLIMLFGLLYAFIFRWFIV"
     gene            9420..10022
                     /locus_tag="PK839_06045"
     CDS             9420..10022
                     /locus_tag="PK839_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HOG72334.1"
                     /translation="MLLQELLTGYKLVLASGSPRRKDLLGGLNIPFEVWPTEHQDESF
                     PSGLDTEIIPQYLAKKKSLAFTGKLDNSTILITSDTIVACNGMVLGKPADMVEASNML
                     KLLSGNMHTVVTGVCISSSSRSATFQSSTNVYFRNLSAEEIDYYVQHYNPLDKAGAYG
                     VQEWIGFVGVERIEGSYFNVMGLPVQRLYIELVSFLQNNS"
     gene            10027..12192
                     /locus_tag="PK839_06050"
     CDS             10027..12192
                     /locus_tag="PK839_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HOG72335.1"
                     /translation="MTKKSLIFFLYFWVTLIPVKADEGMWLPLLIGKNTSNMQALGCK
                     LTSEEIYSVNHSSLKDAVVQFGGGCTAEIISPDGLIITNHHCGFSSIQSHSSVQNDFL
                     TNGFWAMSRSEELPNPGLSVRFLVRMEDVTGSVLQGVSPNTPEAERSRIVSEHIKSIE
                     SAAMAAEPGYLAQVKPFYYGNDYYLLIYEVFNDVRLVGAPPEAIGRFGGDTDNWVWPR
                     HTGDFSLFRIYADKNNKPAPYSPDNVPYKPKKFLKISTKGVNEGDFTMIYGFPGRTQQ
                     FITSDAVSLLVNQSNPHKIHLRDIRLSIFNAYMSGNDTIRIQYASKHAGVANAWKKWI
                     GETVGLQRLNAVQWKLEQEQMFTAWANSNPELSREYAGLLPKFKRLYGELAPLSLVAD
                     YRNEAVYAVELLGFASRFKDVLAEFSDSTLDTVKVASLKNRLSSYASTFYKNFSSRVD
                     MEVFAAMMYQYHQQIGGDYQPQFLTQVNNQQQDSWMQLAYNIYSKSIFTDSLMVLNLI
                     NDMDRSNAQIIASDSIYKIYAQFENIYQSKVDTRYREIQDSIDILYRTYVKGLREMQP
                     DKMFFPDANSTLRVAYGKVAGFSPRDGVNYLYYTTIDGIAQKSLQSDVPDYKPPVKLM
                     ELYRNKDFGRYAVNGTVPVAFIATNHTTGGNSGSPVLDSNGNLVGVNFDRCWESTMSD
                     VMFDPNYCRNIALDIRYVLFIIDKFAGAQHLISEMQLDG"
     gene            12500..13186
                     /locus_tag="PK839_06055"
     CDS             12500..13186
                     /locus_tag="PK839_06055"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic pyrophosphatase"
                     /protein_id="HOG72336.1"
                     /translation="MANRIMDPIERLMGLRYKSHPWHGVDVGPEVPDVVTAFIEMVTT
                     DTVKYEVDKMSGYLRIDRPQKYSSVLPALYGFIPQTLSDESVAQIARGKTDRKDLSGD
                     GDPLDICVLTEKNIVHGNLLVHAIPIGGFRMIDGNQADDKIIAVLKDDAVYGHIQSID
                     QCPEAVITRLRHYFLTYKDLPGTVRDCEITHVYGVEECHQVINASLDDYRKKFENLDI
                     VLSQYGITKK"
     assembly_gap    13436..13535
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(13633..15759)
                     /gene="pnp"
                     /locus_tag="PK839_06060"
     CDS             complement(13633..15759)
                     /gene="pnp"
                     /locus_tag="PK839_06060"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HOG72337.1"
                     /translation="MNIFKKTIELGDGRTITLETGKLARQAHGSVVLTMGKTMLLATV
                     VSAQEAKEDADFMPLSVDYKEKFAASGRFPGGFFKREAKPSDYEVLIARLVDRALRPL
                     FPDDYHAETFVTINLISAEKEIMPDALAGLAASAALAVSDVPFHGPISEVRVARVNGK
                     LLINPSFQQLEEADIDMIVGATYDNILMVEGELKEVSEDEMLEAIKFAHEAIKPQCLA
                     QMELAKEIGVVKRTYCHEVNDEQLKASVKEFCYDKVYSIAKSMLPKQERTAAFEAIKQ
                     SFMETIPEEERESKEVMVNRYYHAVEKEAMRNMILNEGVRLDGRKTNEIRPIWCEVDY
                     LPMAHGSAIFTRGETQSLTTVTLGTKLDEKIIDEVLLQGTEKFTLHYNFPPFSTGEAK
                     ASRGVSRREIGHGNLAHRALKNMIPQGNENPYAIRVVSDILESNGSSSMATVCAGTLA
                     LMDAGIQIKKPVSGIAMGLITEQGSERYAILSDILGDEDHLGDMDFKVAGTRDGITAT
                     QMDIKVEGLSYEVLSKALKQAHEGRMHIMNIMLNTIPAPRADFKPHAPRIEQFEIPRD
                     LIGAVIGPGGKVIQEIQRETSTTINIEEANNHGIVSVFAENAENRDAAVKWIKSIVAV
                     PEVGEVYRGKVKSIMPFGAFVEILPGKDGLLHISEIEWKRLEKVEDVLKEGDEIDVKL
                     IDVDKKTGKLKLSRKVLLEKPATPKK"
     gene            complement(16095..16367)
                     /gene="rpsO"
                     /locus_tag="PK839_06065"
     CDS             complement(16095..16367)
                     /gene="rpsO"
                     /locus_tag="PK839_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HOG72338.1"
                     /translation="MNYLTVEKKKEIFREYGKSDTNTGSSESQIALFSYRINHLTEHL
                     KTHRKDYATQRSLLKMVGQRRRLLDYLKEKDIDRYREVVKALNLRK"
     gene            complement(16938..17525)
                     /locus_tag="PK839_06070"
     CDS             complement(16938..17525)
                     /locus_tag="PK839_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008620744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOG72339.1"
                     /translation="MNANNRSDQELLSEFKEGSNAAMEVLIKRHERRVYTYILLMVHN
                     SHLADDLMQETFVKAIKSLRGGKYSESNRFQSWLLRIAHNLVIDYFRQKSHYREVSNE
                     EYGVDLLNTPRFSEMNAEQRMVYERVIAEVRMMVDGLPPSQREVVMLRYYADLSFKEI
                     ADITNVSINTALGRMRYALINLKKVVKEKNLCLEY"
     gene            17836..20637
                     /gene="uvrA"
                     /locus_tag="PK839_06075"
     CDS             17836..20637
                     /gene="uvrA"
                     /locus_tag="PK839_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOG72340.1"
                     /translation="MTRSVENVISVKGARVHNLKNISLDIPRNALVVITGVSGSGKSS
                     LAFDTIYAEGQRRYVESLSAYARQFLGRMAKPEVDLISGIPPAIAIEQKVNTRNPRST
                     VGTSTEIYDYLKLLFARIGRTYSPVSGKEVKKHSVSDVVNFISSFNEGTKMMLLAPLT
                     PNGDMKQKLEALAHEGFTRVEVGNQIVRIDEQKFREVVDLSGSELRLVIDRFAAAADD
                     EFLSRLADSVQTAFAEGKGFCLLKVFDDNGSYSLEQFSSLFEMDGIKFEEPTEHLFSF
                     NSPLGACPTCEGYGKIVGIDENLVIPNRSLSVYDDAIACWKGESMSWYKDRVVENASR
                     YGIPIHKPYFQLSEEQKSLLWEGTPHFTGINDFFRELEGKKYKIQYRVLLSRFMGKTT
                     CPTCRGGRLRREATFVKVNGYTIQELVEMPVADLAEVFNNLELTEHEVGVAKRILIEL
                     KNRLNYLVNVGLGYLTLNRLSSSLSGGESQRINLATSLGSSLVGSLYILDEPSIGLHP
                     RDTHLLIDVLKKLRNMGNTVVVVEHDEEIIRASDYIVDIGPYAGVNGGEVVFEGPIEN
                     LEKATQSLTANFLLSREQIPVPRVRRKWNSYIEISGARENNLKGIDVKFPLSCLVVVT
                     GVSGSGKSSLVDDILYPALLKQLTGAGPRAGQFDSLGGDWKLISGVELVDQNPIGRSS
                     RSNPVTYIKAWDEIRKLLSEQPYAKRNGYKPAHFSFNVEGGRCEECQGEGVIHVEMQF
                     MADLTLVCESCKGKRFKDDILEVRYNGANVYDILNMTVDQAIEFFSKGKTLTEQKVVD
                     RLKPLQDVGLGYIQLGQSSSTLSGGESQRVKLASFLQKDASIRPLLFIFDEPTTGLHF
                     NDIRQLLNAFDALLAKGHSLVIVEHNLEVIKSADWVVDLGPEGGQEGGLLVFAGTPED
                     LAANEASHTGVFLKKKLNG"
     gene            complement(20682..21122)
                     /locus_tag="PK839_06080"
     CDS             complement(20682..21122)
                     /locus_tag="PK839_06080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72341.1"
                     /translation="MKSFTTTILILGLLLLQPHSTQAQCKNFAKKICKLELAPYVHDG
                     NYNAAILTEGEDAELFKTFYAGQEYRISICGSESLAKIEFLVMDVDRRVLYSNAKNNY
                     ATSWNFKVEATQQLIILVRVPSSGNSGSELASGCVAIMFGLKAE"
     gene            complement(21139..22056)
                     /locus_tag="PK839_06085"
     CDS             complement(21139..22056)
                     /locus_tag="PK839_06085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72342.1"
                     /translation="MATGFVKRIVKLLGLLLIVGGVACKSEKPSDKSLKVTEDELAAD
                     SVISQSIQSVKQIFYSLPSPLETALILKRSGAVYNEDLLNPVENVAHYNTSKSMALNL
                     GIYSTDLSYASLFDQTQATINLMNASKKMADGLGILNAIDNSVIQRLEENINNRDVIM
                     ETISETFLNTNSILEEDDRVAIGSIILVGGWLEGLYLATSLVSDEKKMDNELVDRIAD
                     QKFSLETVLSLLKQNSDNADVMEIYSDMQKLSEIFYDIQISASRVEVVPASNNGATIL
                     RSKNVITVNPDVFNKLKTKVKELRTKYTA"
     gene            complement(22276..22791)
                     /locus_tag="PK839_06090"
     CDS             complement(22276..22791)
                     /locus_tag="PK839_06090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72343.1"
                     /translation="MLAEILKIVLPALMVFLAGYLAISAMIKSETNRRKSELMFNNIK
                     ITTPVRLQAYERLVLFLERISPESLLPRTTKKGMLASDLHTALLGTIRAEWEHNLSQQ
                     LYVSKEAWEMVKNAKENIIKLINVCADRINPNETSMALSQKIIETYVELDTNPTTLAI
                     DFLKKEVAEIF"
     gene            22880..24073
                     /locus_tag="PK839_06095"
     CDS             22880..24073
                     /locus_tag="PK839_06095"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006844052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOG72344.1"
                     /translation="MEKVSSRIAALEESQTIAMSQKSRELAAQGIDVVNLSLGEPDFF
                     TPDFIKEAAKRAIDENFSFYPPVSGYKDLLEAICGKLKRENDLTFTPDQVVVSAGAKH
                     SLSNVLMCVVEKDDEVVVPAPYWVSYVELIKLAEGRSVIVPTTIENDFKITPQQLEAA
                     ITPKTRAFLFCSPSNPTGSVYSKAELEAIARVIEQHPNIIVISDEIYEHINFVGKHES
                     IAQFDSIRDRVVIINGVSKGYAMTGWRIGFSASAKWIAKAAAKLQGQQTSGVCTIAQK
                     AATAAFNSDGQTTRQMCEAFKRRRDLFVKGISGINGFKINVPQGAFYLFPDITHFLGL
                     QFNEQVVATADDFAMYLLNNAHVAVVSGSAFGSPYNIRLSYATSDEKLSMAVERIAQA
                     VKLLR"
     gene            24112..25167
                     /gene="mltG"
                     /locus_tag="PK839_06100"
     CDS             24112..25167
                     /gene="mltG"
                     /locus_tag="PK839_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HOG72345.1"
                     /translation="MSQPQLKTFSRRWLPIVLALAGLFVGFLVLYRYVTTFRVDNVAL
                     THDTYLYIPTGSDFDEVVRDMEGKGLLKNVGSFIKVSRKMGYDLHVKPGRYRVTPDMS
                     NQKLVNKLLMGAQDPVRVTFHNIRTKQQLSGRISKQLEADSLSILTMLRSQVDAMAFG
                     FDTATIMAMFIPNTYEFYWNTDANDFFTRMKKEYESFWTPEREQKAYRLGITRVDVSI
                     LASIVEEETRMVTEMPVIAGVYINRLHRGIPLQADPTVRFALGDFSIRRILSKHLQLD
                     SPYNTYKYKGLPPGPIRCPSIRAIDAVLNAEKHSYLYFCAKPDFSGYHVFARTLMEHN
                     RNAEAYRRALNREKIFR"
     gene            25237..25866
                     /locus_tag="PK839_06105"
     CDS             25237..25866
                     /locus_tag="PK839_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HOG72346.1"
                     /translation="MKQLITPILVCLSGILLAQPAEVLTLNKPSLSRGKNIMEALASR
                     ASATEFTSDELSLQDISDLLWAANGINRPEIGKKTAPSATNSQDIDVYVVTSKGAYIY
                     EPVQHQLKLVNAGDFRELVAGRQKNFANAPLFCIMVSDISRFRGNDDSQKMRWAAMDA
                     AIVSQNINIFCSGVGLRTRTRAFMEADKLREALKLTPSQFPMLNNPVSN"
     gene            26089..26445
                     /locus_tag="PK839_06110"
     CDS             26089..26445
                     /locus_tag="PK839_06110"
                     /inference="COORDINATES: protein motif:HMM:NF039670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6150 family protein"
                     /protein_id="HOG72347.1"
                     /translation="MFRFVKILLTLVAVLLAEITFAQKFFEVEKVEDAQVKFYAVDDP
                     KDADLLFCIIYEEKLITKAGIMMEVETPREAQVLIIFVDDPALADIKVWLVEKPEEVV
                     WKNESKRKLLSVGGLK"
     gene            26523..27692
                     /locus_tag="PK839_06115"
     CDS             26523..27692
                     /locus_tag="PK839_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011966691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HOG72348.1"
                     /translation="MNDQHRYNQRGVSASKEDVHNAIKNLDKGLYPKAFCKVVPDILA
                     GNPDYCLVMHADGAGTKSSLAYAYWRETNDMSVWKGIAQDAVVMNLDDLLCVGVTDNI
                     LLSSTIGRNKNLIPGEVISAIVEGTAQFCEEMQKYGIHITLTGGETADVGDLVRTVIV
                     DSTVIARLKRAEVISNSNIQDGDVIIGLSSCGKANYEQEYNGGMGSNGLTSARHDVFS
                     SYLADKYPETFDPSVPRELVYSGSFKLTQMVDEVGITAGKLMLSPTRTYAPVVKEVLD
                     SYRPVIHGLVHCSGGAQAKVLNFVDNLHVVKDNLFPIPPLFRIIQSESNTSWREMYKV
                     FNMGHRFEIYVFPEFADDIIAISKDFGVDARVVGYCSSFQGKKLTIKSEKGEFYY"
     gene            27941..29029
                     /gene="prfA"
                     /locus_tag="PK839_06120"
     CDS             27941..29029
                     /gene="prfA"
                     /locus_tag="PK839_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HOG72349.1"
                     /translation="MSDNTITRKLEGVRQKFEEIGQKITDPAVMADMKKFVSLNKEYK
                     ALEPLVAAYEKYKNILSNIESSRQILSSEKDEELREMAKEELDALTQEQEKLEEEIKL
                     LLLPADPEDSKNAVMEIRAGTGGDEASIFAGDLFRMYTKFFERKGWKYEVTSSSEGTV
                     GGYKEIVMSVTGNGVYGILKYESGVHRVQRVPQTETQGRIHTSAATVAVLPEAEEFDI
                     DLKMEDIRKDIFCSSGPGGQSVNTTYSAIRLTHIPSGIVVQCQDQKSQLKNFDKALAE
                     LRTRLYNLEYQKYLDNLSSKRKTMVSTGDRSAKIRTYNYPQGRVTDHRINLTLYNLSA
                     IMDGDIQELIDKLQLAENAERLKAESTD"
     gene            29042..29875
                     /gene="pyrF"
                     /locus_tag="PK839_06125"
     CDS             29042..29875
                     /gene="pyrF"
                     /locus_tag="PK839_06125"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HOG72350.1"
                     /translation="MTPEQLFDQIRLKRSFLCVGLDTELTKIPEFLLTKDDPIFEFNK
                     AIVDATHDLAIAYKLNLAFYEVHGATGWNSLVKTIKYIRRFYPSMFIIADAKRGDIAS
                     TARMYANAYFKSLDCDAVTVSPYLGHDSVTPYLNFEGKWTILLAITSNDGFRDFQLAV
                     NRDTGNFMFEDVVAKSLQWGNEANIMYVVGATHPEYFAAVRKIVPNHFLLVPGVGEQG
                     GSLEKVAEHGLNNRCGLIVNSSRGIIYADITSRFTGAARARALKLQQQMESILTAAKL
                     V"
     gene            complement(29936..31072)
                     /locus_tag="PK839_06130"
     CDS             complement(29936..31072)
                     /locus_tag="PK839_06130"
                     /inference="COORDINATES: protein motif:HMM:NF019342.2"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HOG72351.1"
                     /translation="MRIAQIRLLAASMLITTSVLHTGCSTNTRSSEEITIGVEELCSK
                     DGKSLIITHPTVSTIKTFDKLVKNGLLVLPNRTRIVGIYHSGENYDYALSQQYIAENA
                     IDYFYLLPIKPEITPDSIFHRNACSGIFEKLVSTCAGIIFTGGPDIPPSIYNEPTSLL
                     TQITDPERHSMEISFLFHLLGGRRDTLFKPLLADSSNLAVLGICLGMQSINVAAGGTL
                     YQDIPTDIYHLSTVESVLAQEINQQHRNYNCSYGYEEQLLGGTFHQVEFSDELFSSIA
                     AGKKPFVLSSHHQCLKMLGDNIRVAGYSADGKVVEAIRHIKYKNVVGVQFHPEASLLY
                     DRNELFLITPNQQSGQSYLNLYPGESGENFHREFWRWFSALLVK"
BASE COUNT         8026 a         6862 c         7192 g         8945 t
ORIGIN      
        1 cagctgcaaa caagatggtg gaaccgaccc accagagtct ccttgcagga ggcgttctaa
       61 actacttctc taatgcatgg cgggcaatcg gtgtgtgtga agcgagagaa gaaaaggcta
      121 agctgttacc ggtcaggtat ggataagaga gatgagcggg agcgaaccct tgatgacgca
      181 tcggtaaccg atccgccaac tggcggagag ctcggcgaag tctgtgactt cgtagtactg
      241 aagtagcagt cgctgaccgc gtcaaatcct tgctaaagtt ccggactcca gaccgaaaac
      301 tactaacaac gaaaaacaaa ctttgcctta caacaagaca gttgtaagcc gcctttagcc
      361 acgacttagc taccctttgc tcaaacccta gcgcttaaac ctgcttcgaa cggggaaata
      421 tcagccatag gtatgcataa aagggtaacc gaaatcgatt acccttttta atgactttga
      481 aaatgcctaa atgaaatgtc aggattttag cattcttact cccctcaatt agaacttata
      541 cacattatcg gcaatcagct tatcggcaat ttttctacgg gcttccttaa cattaatggg
      601 gtttactgcg cttaggcgtt cggtagcagc aatcagctta cctagttctg gctcagcagc
      661 aaaggataat atggcatcaa tggcattttt acggattctg gaggctgcat caaaaataaa
      721 aacgtccaga atactcctgt atatatccat tgaagcatga cctctgctct ctagtttttc
      781 gacccttagg gcaagcgatt cggcaacgta aatatccatg atgatgttgc tgaggttatt
      841 ttgaacctcc tgctcgctta caaactgctt tgcaaaagtt ttgttggcac caaaaatccc
      901 cagcagggca atctttttga agttcctgat gagctgtttt ttctgctcaa agtaaccagc
      961 agcacccatt tctaccttaa ttccatccag gttggtgtaa agttcctcgg ctggtcccat
     1021 aaggttgaag tcgcctttca gcgagcgctt tattgctgtg tcaactacca gcaggcggtt
     1081 aatttcgttg gttccctcaa aaatccggtt gatacgggag tcgcggtagc ctctttccac
     1141 attcatttcg gcagagtagc ccattccgcc atggatttga acaccctcat caactacaaa
     1201 gtcgagcatt tcggaaccgt ttaccttcag aatggcatcc tcaacagcgt agtggcttat
     1261 ggcatcaata gctgccttgc cgtagtcgca accttcggct ttgtagcgtg taatcagctc
     1321 gtcgatgttc tgactaaccc ggtagattgc cgattcgtgg gcaaatgttc tgataacctg
     1381 ctcggccagc ttatgcttaa tggagccaaa gttggctatt tgcgtgttga actgcttgcg
     1441 ctcattagcg taacgaaccg attgggttat ggtttccttg gctgccccaa taacgttagc
     1501 ccccagcttc attcgtccca tgtgaagaat gctaagggca attcggaacc cctcaccacg
     1561 gtttcccaac aggttttcga ccggaacttt cacatcgtta tagtagattt gagcggtaga
     1621 ggatcctttt attcccatct tgtgctcatc ggggccaacc acaacgcccg gccacttact
     1681 ttccacaatg aaggcgctta gcaccctgtc gttttcaatt tttgcgaata ccacctgggt
     1741 atcagcaaat ccggcgttgg taatccacat tttctggccg ttgagaatgt agtgcttgcc
     1801 atcttcagaa agcttagcgt tggttttgcc agagtttgca tcgctgccgg ctccaggttc
     1861 tgttaagcag tatgcaccaa gtagctcgcc ggttgcaagt cgggttacat acttctggcg
     1921 ctgctcctcg tttccatagt atagtattgg catggtacca attccgcaat gggccatgta
     1981 ggccacagaa aatgagtagc cagcaccggt tgtttcggca acaagcattt gcgtgatgaa
     2041 cgattgtcca aaaccgccat actcttctgg aactgctatg ccgagcaacc cgagctcacc
     2101 tgtttttttc agtaagcttt tcatcagctc cctgtcgctt ttatccaggt catcaagctt
     2161 ggggtaaacc tcagcctcaa gaaaatcgcg acatgtttgg gcaatcatcc tttgctcctc
     2221 gttgaactct tcagggataa aaatatcgtt ggcttcaact tcgtttacca gaaattcgcc
     2281 acttttaagt agtttactct cactcatggt ttgcttggtt tatgttttgt agtctataga
     2341 tttaacgatt cggcaataag ctccgctatg tcgaggttac gaatcttctc ctcctggttc
     2401 ttatacttaa taccatctgt tatcatggtc atgcaaaacg gacatgcggt tgctatgatt
     2461 tcggggttca ccttcagcgc atcctcggtt cgctcgatga aaacctcctt gtttcctttc
     2521 tctgcctcct tgaacatttg tgcccctccg gcaccgcagc aaagggaaaa actccggttg
     2581 cgctccattt caacaaattc gccagtcagg gttttaatta ttctgcgggg ttcttcataa
     2641 atgccgttgg cccgacccag gtagcatggg tcatggtaag ttatacgggt gttcttgaat
     2701 ttaccggcat caagttttag cttcccctcg gttatgtact gctccagcag ttgggtgtag
     2761 ctaataacct cgtagctacc gcccaaatcg gggtactcgt ttttaaaggt gttaaagcag
     2821 tgagggcaga tggtgataat ttttttaata tcgtatgact tgaacagctc aatattttga
     2881 aatgcctgca tttggaacaa catctcgttt ccggcccttc gggcaggatc acccgtgcag
     2941 gtttcctctt ttcccaacac ggcaaagctg atgttcaggt gaaccagaat tttggtaaat
     3001 gcccgggtaa ctttcttgta cctatcgtcg taggctccgg cacatccaac ccagaacagg
     3061 tattcgggtt tttctccgcg ggcgaaaagt tcggccatta caggcacttc tatttttttt
     3121 gtttccatct ttatggctgg ttattcaaat ggttgtgttt gcattgtaat gggaaaaata
     3181 cttattgtat gttgatttcc aaattgttag cccagttaag cctgtcttct ggtgagtact
     3241 gccagggtgc tccgttgttc tcgatattgt taaacattgc ctttatttct ccgggtgctg
     3301 agccctcctc catgaccaga tagcggcgca tatccacaat caaggtggga tggttaatgt
     3361 tgattggaca ctcctgggcg caggcgttgc aggtagtaca agcccagagc tcctcctcgg
     3421 agatgtagtc cctaatcagc gatttgccat cgctgtaatc ccttccattt ttcagcatta
     3481 atgggccttt ctccttcatc cttgcgcgca ggtccatcat taccttgcgg ggtgaaagcc
     3541 ttttgccggt taggtttgcc gggcaaacgg aggtgcagcg tccgcattcg gtgcaggaga
     3601 gggaatcgaa gtagcttttc caaacgacat cctcggcatc ctttacgcca aatctttcga
     3661 ttggtgtgtc ggaaggaggt gcggcaaagg cagcgttcgg atccagcata agcttaacct
     3721 cgcgggtaat gctctccatg ctgggtagct tgcccagtgg ttccagcctc gacagaaaca
     3781 cgttagggat ggacatgaac acatgaaagt gcttggagta ggggagtatg ttggcaaaaa
     3841 gaaaaatgag cagaatgtgt gaccaccaaa agacttcgta gtaagtgtaa atgacctcta
     3901 ccggcatgga ggaaaacaga ccggtaaggt gctggcttac cggataaact ccatgaatct
     3961 cctttccaag agaatggcac cagctcacgt aggcagtgtt catccccata agggaaatca
     4021 ttaaaagcaa aattagcgta agggcgatgt tggcatctat gtgcgaaatt ttcttcatct
     4081 cgatgccctc gaagcgttta atatgcagga ataccctgcg tattaaaaaa accacaatgg
     4141 caatggctat gataggtgca aagatatcgc cggcagccat gatgaagtcg tgaaaccaac
     4201 ctaaaacgct cagcgacttt tcaagcccga acagcccgtc aatgaccatt tcgatgcttc
     4261 caaagatgat taagcagaat ccccagaaaa ccatggcgtg tagaaaccca atgatgggcc
     4321 gacgaaaaat tttggtttgg ccaaatgcca ccttgagcat tatcccaata cgttttccaa
     4381 tatttttaac cggaaacccc ttacgggtta acgagaaaaa ccggatgatt ctggttatgg
     4441 taaaaccaaa agcaaccagc gttaccagca atgctaatgc aaaaacaact tgtttggtca
     4501 tggagcttca gttttttatt attctattgt ttcgtcgggt ttgcaggtaa tgcaatggtg
     4561 tatgccgcct atgttcatat catgggctat tttatggaac ttcgtgcaac cggacatcct
     4621 cctgctgcgt agtgtggatt taatcacctg aatctattca ttaaacataa ccttacatgc
     4681 aggagcactg cttgtttatc tcctgcatgt tgttttttta ggcagcaatt ttttctgctc
     4741 gtggagccaa aacaccaata ttggagctgc caaacatcgg cattggctat tttcctttaa
     4801 tttccctaac agcttctacc agtttgggaa gaaccttttg ggcgtcgcct acaatgccgt
     4861 actgggctac ctcaaatatt ggagcactct tatcgttatt aatagctaca atatactttg
     4921 aaccgctcac accggcaata tgctgggttg ctcccgagat accaactgca atgtaaaggt
     4981 ttggggcgat tatttttccg gtttgcccgg tgtgctcctc gctgggacgc caaccttcat
     5041 ctgaaaccgg acgcgagcat gcggtagcac ctccaagcag ttcggccagc tccaccagtg
     5101 gtccccagtt atcgggcgat ttcattccgc gtccgcccga aactacaatc tcggcatcag
     5161 tgagcagcag tttgcctgct tgcttctgta catcctttac cacggtttta ggttcgggta
     5221 ggctgatgct aattttttcc acttcagcgt taaccggagt ttcggttaca tcaacagagt
     5281 tttggggtag ggtaactatt tttacgtcgg tttttaacac aacgtgggca atggcaccac
     5341 ccgaaaaagc tctcttacga acggtaaagg gattggttga tagcggtagg ccatttacac
     5401 ctgctgctag cgcagctttt aactttacgg ataatctagg cgcaacggct ttgccaacat
     5461 tattgttaga aattacaact acttttgcat cgttgttttt tgcaacctgg gctatagctt
     5521 cggtgtagtt ttgtgtgtcg aataccttca ggttgtcact gtttacgctc aatatctttt
     5581 gggcgccata gctacccagc tttttcaact cggcatcatc aacatttcca atcgaaaggg
     5641 caatgcagct ggtattcagc atttcggcaa cccttgttgc atatgatacc agctcaaagc
     5701 ttagcttttt gaattttccg tcccaatttt ctgtatatac tagtactgac atatcgtaat
     5761 cgtttaatgg gtgaaggcta aattaacttg gtttcggttt gtaggagttc gatgagctgt
     5821 tttggatttt cagcatcaac aaacttacaa gctgcgcgtg gtttgggtag ctcgtactta
     5881 acatattcgg tcatgatttc tacaggaaca ggctcgtaaa gttttattgg cttagtgcgt
     5941 gctgtcataa taccgcgcat tgcggcaatt cttggttcct tagctattcc cttttgaaca
     6001 acggcaacta atggggtggg ggtggtaacc acttcttttc cgccatcaac ctcgcgggtg
     6061 ataacaactt gtccgttttc tatcttgagg tttgaaactg ccgatatgga gggtattcct
     6121 aaaaattcgg caagcatacc tccaaccgaa gatccattgt aatcgctcga ctcgattcca
     6181 catagaataa ggtcaaactg ttccttcctt accacctcgg caagctgagc agccacaaag
     6241 taggcatcgg acgagtcggc attcaccctg atggcatcgt cggctcctat agctaacgcc
     6301 tttctgatgg ttggttccga tgaggataaa cctacgtggg ctacggatac gcttttcacc
     6361 ccggtgttac catcatcctt caactccaac gctctggtga gcgaaagttc atcccatggg
     6421 ttaataaccc actgaatacc gttggtatcc agcttgctgt ctccctctgt aaacttcacc
     6481 ttggtggtgg tgtcgggtac gttgctgata cacactaaaa ttttcatcag actatagtat
     6541 aagttttaaa atgtattggt ttactacttt acacttgaag gtttcttgat tagaattgca
     6601 aaaataatca aagttattaa tgggaaaaat ttgttgggtg gggtttatac ccactgtcgt
     6661 caaaattcac tgaagttgag taggttatgg cgcttttttg gagttgagca ggaagtgaag
     6721 cgatggccaa tgacgtagct gtgaaccggg agagggaaga agctaaggaa agaggttgag
     6781 ctatatgaac catgaatccg tcagatccca acgcctgttt gctaaaagcc aggcaagttc
     6841 agttaagaac cggacctgcc cggacgctga gcctgtcgca gggtaaactc ctcggaatga
     6901 gacaagcttt cgtcaaaagc cactcaggct tcgattttct ttatcccttg ccttaattct
     6961 tgaatgtcct ttctccagta accttatttt attagctatc tttatgcttt aaaatatatg
     7021 gtgatgacca aggctgattc cttcaatagg gatgttgttt ttattggtgc tggcaatctg
     7081 gccacttcac tttctgttgc cttttttaat gccggttggc gtgtggttca ggtttactcc
     7141 cgaacctacc aaagcgctgc cacccttgcc aaccggctca acgcagagcc aactgccacc
     7201 ctcaattcgc taattcgcga tgcccagcta tacgtggttg cccttcccga ctcggtaatt
     7261 caaaccgtag ccgaacagct tccgtctctt aaaggtttcc tggtacatac gagtgggagt
     7321 acatcaattg aagtgctttc ggttgctgat gttctgggat acggggtatt ttatccgctg
     7381 caaacatttt caaaggccag gctggttgag tttaaaaatg taacggtttg cattgaggga
     7441 aatagtcagg caaccctaaa cgccttaagc agtatagccc aagctattac atcatcggtt
     7501 gtagctctcg attcggagaa acgcaggtgg ttgcatcttg ctgctgtttt tgcctcaaat
     7561 tttaccaacc acatgctggc tgcagcatac aggattgccg agcagaatgg agttgatttt
     7621 aagatttttg agccgctagt tagggagact gtttcaaaag cttttcttgc caatccaaag
     7681 gattttcaaa cggggcctgc tgtacggttc gaccagcaaa ccatggagca gcatctctcc
     7741 atgcttggat tttctgactt ggagttgcag aatatttaca aggagctatc tttgagcatt
     7801 cagaattttg cccaaagtaa aaagtaatgc ccatgcgtaa aaattttaag gagatgttgg
     7861 ccctggtaaa ggcttttgtt ttcgatgttg atggggtttt taccgatgga acaataatta
     7921 ttcacccttc gggtgagctg ctgagaactt caaatacccg cgatggctat gctgttgggg
     7981 tagcggttgg aaggggtttt cctgtggcta ttatttccgg cggaaaatcc gatgccgttg
     8041 gggttcgttt cagggggctt ggtgttcagg atatttacct tggggtggcc gacaaaatga
     8101 ttgctcttga agaatttcgt aaaaagtatg acctggagct ggctaacctt ctttacatgg
     8161 gcgacgacct acctgatctt gaggtaatga agctggtggg cgttccaact tgtccccatg
     8221 atgccgccta cgaggttaag caggttgctg tttatatctc ctcatttaac ggaggcaggg
     8281 gatgcgttcg agatgttatt gagcaggtgc taaggctcaa tggtaagtgg ggcgaaagtt
     8341 ccgacatgta catgcagtaa ccctactgct tttataggta tgctgaaaat caagtggaat
     8401 aaaacaacaa aataggctac ttttacatca caccagctat tttaacttga cgtatgaagt
     8461 tttttcaact aattcggtat aagaacctgc ttatcattat cgccacaatg atactgatga
     8521 ggtactttat tattgaacca attttgctaa gctatgggtt ggcgcttcag atgccaaccg
     8581 gcgtttttat tatgcttgtc atggctacgg tgcttattgc tgccggagga tatgttataa
     8641 atgattattt tgatacccgt accgatttgg ttaacaagcc tggagaagtt attgttggac
     8701 aggaggttac ccgtcgggag gcaattgcca tgcacatggt gctaagcaca ctcggcattt
     8761 tgctggggct tgtagtatca ttcaaggtgg gtcgtcctgt tttttcgctt ctctttttcc
     8821 tcactgccgg aatactatgg ttctactcca ctctgtacaa gcgccagctg attgtcggga
     8881 atcttgttgt ttcggtttta acaggattaa ttcctctcat tcccctggtg tttgagcttc
     8941 ccttgttggg aaacttctgg gaggttattg ccgtttacca gctcgacctg caccagcttg
     9001 tttactgggt tggtggttat gccacaatgg ctttcctact cacatttgtt cgtgagatta
     9061 taaaggacat cgaggacttt gaaggggatt ttacctatgg tcgtaacacc attcctgtcc
     9121 gatatggtat caaaacagcc aaggttacag cagcttccat gcttggaatc accatgctgg
     9181 ctctggttta cctttttgcc gcttacatgc gtcagcttag caccggcgaa ttcgattttg
     9241 ttactttttc ctacttcttt ttattcatag ttgttccatg ctttgtgctg ctggtcatga
     9301 tacttctggc gcaggaaaag aaggactacc acagggcaag tggcttttgc aagcttatca
     9361 tgctatttgg ccttctctac gcatttattt tccgttggtt tattgtttaa cttttattga
     9421 tgcttttaca agaacttttg acaggataca agctagtgct tgcctcgggc tctcccagac
     9481 gaaaagattt gcttggggga ttgaatatcc catttgaggt atggcctacc gaacatcagg
     9541 atgagtcttt cccttcaggt ttggacactg aaataatacc ccagtaccta gctaaaaaaa
     9601 aatcgttagc ctttactggc aaactcgata attcaactat actgataacc tccgacacaa
     9661 tagtagcatg taacggaatg gtgctgggta aacctgctga tatggtggag gctagtaaca
     9721 tgttgaagct cctttcgggc aacatgcata ccgttgttac tggagtctgc attagctcca
     9781 gtagtaggtc ggctactttt caatcctcca cgaatgttta ctttcgaaat ctttctgccg
     9841 aagagataga ctactacgta cagcactata acccacttga taaggctgga gcctatggag
     9901 tacaggagtg gattgggttt gtaggtgtcg aaagaataga aggctcctac tttaacgtaa
     9961 tgggattgcc cgtacagcgc ctatatattg agctggttag ttttttacaa aataatagct
    10021 aaaactatga ctaaaaaatc attaattttt ttcctgtatt tttgggtaac ccttattccg
    10081 gttaaggccg atgagggtat gtggcttccc ctgctgattg gaaaaaatac ttccaacatg
    10141 caggccctgg ggtgcaagct aacctccgaa gaaatctaca gcgttaacca ctccagcctt
    10201 aaggatgccg tggtgcagtt tggcggtggc tgcactgccg aaattatttc acccgatgga
    10261 ttaattatta ccaatcatca ttgcggattt tcttccattc aatcgcactc atcggtacaa
    10321 aacgattttc ttacgaatgg tttttgggcc atgtctcgct ccgaagagct gccaaacccc
    10381 ggattatccg tccgtttcct ggttcgaatg gaggatgtta ccggttcggt gctgcaaggt
    10441 gtttcgccca atacgcctga agctgaacgg agcaggattg tttctgaaca cataaagtct
    10501 atcgagtctg ctgctatggc ggccgaacca ggatacttag ctcaggtcaa gccattttac
    10561 tatggaaacg actactacct tctgatttac gaagtcttta acgatgtaag gttggtaggc
    10621 gcaccacccg aagccatagg ccgttttgga ggcgataccg ataactgggt atggccccgc
    10681 catacgggtg atttttccct gtttagaatc tacgccgata aaaacaacaa gccggcaccc
    10741 tactcacccg ataatgttcc ttataaacct aagaaatttc tgaaaatttc aaccaagggt
    10801 gttaatgagg gtgattttac catgatttac ggtttcccgg gtagaactca acagtttatt
    10861 acatcagatg ctgtaagcct gctggttaac cagagcaatc cccacaaaat tcacctgagg
    10921 gatatacgtc tttcaatttt caatgcttac atgtctggca acgataccat ccgaatccaa
    10981 tatgccagca aacatgcagg ggttgccaat gcatggaaga aatggattgg cgaaactgtt
    11041 gggttgcagc gcctgaatgc tgttcagtgg aagcttgagc aggagcaaat gtttaccgca
    11101 tgggcaaatt caaatcctga actttccagg gagtatgccg gcttactccc taagtttaaa
    11161 aggctctacg gggagttggc acccctttcg ctcgtagccg actacaggaa tgaggcggta
    11221 tatgctgttg agttgctcgg atttgcttca cgttttaaag atgttttagc tgaattttcg
    11281 gacagcacgc tcgacacggt taaggtggca tcgcttaaaa acaggctgtc ttcctatgct
    11341 tctacatttt acaaaaattt tagctcgcgg gtagatatgg aggtatttgc agccatgatg
    11401 taccaatacc accagcaaat cggtggcgat taccagccac aatttttaac tcaagtcaac
    11461 aaccagcaac aggatagctg gatgcagctg gcttacaaca tttacagtaa gtcaattttt
    11521 accgacagcc tgatggtgct gaacttgata aatgacatgg atagaagcaa cgctcaaatt
    11581 attgcatccg acagcattta caagatatac gctcagtttg agaacatata ccagtctaag
    11641 gttgatactc gctatcgtga aattcaggac agcatagata ttctataccg aacctacgtt
    11701 aagggactca gggagatgca gcccgataaa atgtttttcc ctgatgccaa ctctaccctt
    11761 agggtggctt atgggaaagt tgccggattc tcgcctcgcg acggggtaaa ttacttgtat
    11821 tacaccacaa ttgacggtat tgcccagaaa tcactgcagt cggatgtccc cgactacaaa
    11881 cctcctgtca agctgatgga gctatatcgc aataaggact ttggtaggta tgcggtgaat
    11941 gggacagttc ctgttgcctt tattgcaaca aaccatacaa caggtggaaa ctccggaagt
    12001 cctgtgctcg attcaaatgg aaacctcgtg ggggttaact ttgaccgatg ctgggaaagt
    12061 accatgagcg atgtgatgtt cgaccccaac tactgccgaa atattgccct ggatattcgc
    12121 tacgtgctgt ttattattga taaatttgcc ggagcacaac acctgattag cgagatgcag
    12181 cttgatggct agctcctaaa gctatcagaa attttttgaa ttacaagttg ttctatctta
    12241 gagtataatc taatttcatc ataggtgaaa aggcttacac gtttaacggg ttcaggctaa
    12301 ccgatgagtg cctttgcctg aattatacca aatcactaaa gcgtccctgt ccctggacga
    12361 aggaatatta agatagctta tgggaatttt actacatgta aggtttaaag catttctttt
    12421 gagaaattgt atgtatatgg tgttgaggtg ttggagcgta aaagctgttt ttaaaacttt
    12481 atcctgaaaa aagcattgta tggcaaatag aattatggat cccattgaga ggttgatggg
    12541 tttaaggtat aaatcgcacc cctggcatgg tgttgatgtt ggtcctgaag ttcctgatgt
    12601 ggttactgcc tttatcgaaa tggttactac cgataccgtt aagtacgagg ttgacaagat
    12661 gtcgggctac cttaggattg atcgtcctca gaagtactcc agcgtgctgc ctgcgctcta
    12721 tgggtttata ccccaaaccc tatcggatga aagtgtagcc caaatagcac gcgggaaaac
    12781 cgaccgcaaa gatttatcgg gcgatggtga tccgctggat atttgtgttc ttacagaaaa
    12841 aaacatagtc cacggtaacc tgctggttca cgcaatccca attggtggct ttaggatgat
    12901 tgatggcaat caggctgatg acaaaataat tgcagttctt aaggacgatg ccgtttatgg
    12961 gcatatacaa agtatagacc agtgtcccga agcggttatc accaggttaa ggcattactt
    13021 ccttacctac aaggatttgc caggcacagt acgcgattgt gagataaccc atgtttacgg
    13081 agtggaggag tgccatcagg ttattaacgc atcactggac gactaccgaa aaaaatttga
    13141 aaacctcgat attgttctat cccagtacgg catcactaaa aaataaatac ggaggtgctg
    13201 ttttacttgc cgattaccct gtgaagcaaa atttgaattc acttaaaaca atttcctggg
    13261 gatggggtca ttatcaatgt gaattactca tccacctatt tttcgaaagt gctaacaagt
    13321 taagaaaagc caggatgccc tggctttaca cactttttgg gacagtatca actttaaggg
    13381 ggaaaatcgg attgcaggag gtaaaattcg taaaaaaaac ctgccagaag tttgcnnnnn
    13441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnggaaa atcggattgc aggaggtaaa
    13561 attcgtaaaa aaaacctgcc agaagtttgc tggcaggctt tttaatacta tgagtcattt
    13621 actttgaatc acttactttt taggtgtggc gggtttttcg agcagcacct tgcgtgagag
    13681 tttaagcttg ccggtttttt tatcaacgtc aatcagctta acatcaatct catcgccttc
    13741 cttcagaaca tcctccacct tttccaggcg cttccattca atttccgaga tgtgtagaag
    13801 tccgtctttt ccgggtaata tttccacaaa cgccccaaaa ggcatgatgg atttaacctt
    13861 ccccctgtaa acctcgccta cttcaggaac cgcaactatg ctcttaatcc acttaactgc
    13921 agcatcgcga ttttcggcat tttcggcaaa tacgcttaca attccgtggt tgttagcctc
    13981 ctcaatgttg atggtggtgg aggtttcacg ctgtatttcc tgaatcactt tacctccggg
    14041 accaataacg gcaccaatca ggtccctggg aatttcaaat tgctctattc ggggcgcatg
    14101 gggtttgaag tcggcacggg gagccggaat ggtgtttagc atgatattca taatgtgcat
    14161 gcggccttca tgagcctgct taagagcttt tgaaagcact tcgtaggaaa gtccttccac
    14221 cttaatatcc atttgggtgg cggttatccc atcacgcgtg ccggcaacct taaagtccat
    14281 atcgcccagg tgatcctcgt cgccaagaat atccgaaagg atggcgtaac gctctgaccc
    14341 ctgctcggtg atgagcccca tggcaatgcc cgaaaccggt tttttaatct gtatgcccgc
    14401 atccattagc gccagcgtgc ctgcacatac tgttgccatg gatgatgacc cattggactc
    14461 cagaatgtcg cttacaactc ttattgcata aggattttcg tttccttgag gaatcatgtt
    14521 ttttaatgcc cggtgggcaa gatttccgtg cccgatttct cgacggctaa cccctctgga
    14581 ggcttttgcc tcaccggtgg aaaagggtgg aaagttgtag tgaagagtaa atttttccgt
    14641 accctgaagc agcacctcgt ctattatctt ctcgtcaagc tttgttccta acgtaactgt
    14701 ggttaacgac tgggtttcac cacgggtgaa tatagccgac ccgtgagcca tgggaaggta
    14761 gtcaacttcg caccagatgg gtcgaatttc gttggttttt cgaccatcaa ggcgaacgcc
    14821 ctcgttgagt atcatgttgc gcatggcctc cttttcaact gcatggtagt agcggttaac
    14881 cattacctcc ttcgattccc gttcctcttc cggaatagtt tccatgaacg actgcttgat
    14941 ggcttcaaag gcagctgttc gctcctgctt tggcagcatg gatttggcaa tgctgtaaac
    15001 tttatcgtag caaaactctt ttacactagc ttttagctgc tcatcgttta cctcgtggca
    15061 atacgtacgt ttaaccacgc ctatctcctt ggccagctcc atttgagcca agcactgggg
    15121 tttgatggct tcatgggcaa actttattgc ctcgagcatt tcatcctccg aaacctcctt
    15181 tagctcccct tcaaccatga ggatgttgtc gtaggttgcc cctacaatca tgtcgatgtc
    15241 tgcctcctca agctgttgaa aggatgggtt gattagtagc ttgccgttca ctctggccac
    15301 gcgaacttcg gatatgggac catgaaatgg aacatccgat acggcaagag cagcggatgc
    15361 tgccaagccg gcgagtgcgt cgggcataat ttccttttcg gccgaaatta ggtttatggt
    15421 aacgaaggtt tcggcatggt agtcgtcagg gaataacggc cgaagggcac ggtcaaccag
    15481 acgggcaata agcacctcgt aatcggaagg ttttgcctca cgtttaaaga aaccacctgg
    15541 aaaacgtccg ctggctgcaa atttttcctt gtagtccacg cttagcggca tgaaatctgc
    15601 atcttctttt gcttcctgag cgcttacaac tgtagcgaga agcatggttt tacccattgt
    15661 tagcacaacc gagccatgag cctgcctggc caactttccc gtctccagcg tgatggttcg
    15721 accatcgccc aactcgattg tttttttaaa aatattcatc tgcttgtgtt tatttaattt
    15781 gttgtgtttc aaaaaaaggt tttaagaagt tctcgttaaa ggtagaaata ctatatactt
    15841 gtcattttaa cctttgccaa tcatgatagg gtcggtatgg ataatcctca tggtcgcaca
    15901 gctataaccg agtatttgtg cggtatggga aaatcatttt aaaaaatatt gttctgccgt
    15961 gatattctgg tttaaaaaat ttcgtttaga aatgctagct atattaaaaa aagtagaggc
    16021 aatttactct tccatttttg atttaagggg tagattgtaa aaaaggcaat tttttaaaat
    16081 tgccttttta agttttactt tctcaaattc agcgctttaa ctacctcgcg atacctgtca
    16141 atatctttct ccttcaagta gtccagcaac cgcctccgtt gaccaaccat ctttaacagc
    16201 gaacgctgtg tagcgtaatc ttttctgtga gttttaagat gttctgtgag atggttaatg
    16261 cggtaggaaa acaatgcaat ctggctttcc gaagaaccag tgttggtatc ggacttacca
    16321 tactccctaa aaatttcctt tttcttttct acggtcaaat agttcatagc actaataatt
    16381 agtgagtaga tatacactta ttttgaggtg caaagatagg agttttttta cattttgcct
    16441 atacacttaa tatttttttg aaaagttctg aagatgatgg atatctgaaa aaattgaagg
    16501 acaatggata aggagaattt gtttggattt ggctgataaa tccttcaacc aggctctgga
    16561 caggctaaaa tcttctagct ttagctattc ctgtccccat tttaaacttt ttcagggtgc
    16621 tcgcctctta gaaggagaat tttcccatgg cgtaagtgaa attgatggtg aaccacccgt
    16681 gtaggacaac ttgcacaaac ctgcatgaac aaccggcctt tacctgcaaa ctcggtgaag
    16741 ccaaaatacg aatgttccat tacattgacc ccgaagccta aaagtagggt aaaggtaaaa
    16801 gtaatcagta accggaaacc taataatttt gctatgctaa ggctctacgt caaaaagtta
    16861 agacttgttt cgttttgctg catcaaccct aattagctcc ctgccagggt tgagaagcaa
    16921 ccgatcctgg cagctggtta gtattcaagg caaaggtttt tctccttcac cacctttttt
    16981 aggtttataa gtgcataccg cattcttcct aaggcagtgt taatgctcac gttggtgata
    17041 tcggctattt ccttgaaact caagtcggca tagtaccgga gcattaccac ctcacgctgg
    17101 ctggggggta gcccatctac catcatcctt acttcagcga ttacacgttc gtaaaccatt
    17161 cgttgttccg cattcatttc agaaaaacgg ggcgtgttga gcaggtcaac tccatattcc
    17221 tcgttagaaa cctcacggta atggcttttc tgtctgaagt aatcaataac caggttatgc
    17281 gctattcgca gcagccacga ttgaaaccgg ttgctctccg aatacttgcc cccccttagc
    17341 gactttatgg ccttcacaaa agtctcctgc atcaagtcat ctgccagatg cgagttatga
    17401 accattagta aaatgtaggt gtaaactcta cgttcgtgcc tcttaattag cacctccatt
    17461 gcagcattgc tgccttcctt aaattccgat agaagctcct ggtcggaacg attgtttgca
    17521 ttcacagcaa cctcccatgt attggtaatc gggtagagct tcttccaata ggcaggtgtg
    17581 atttaggtta gttaacttaa gttacaatcg tttgccaaaa aaagtatttt tttttgatat
    17641 gtgcaagttg aacccatttt tttattatca cgtggctgtt tattttctta cttttttttg
    17701 agggaaggtt caaaggaaat tcctcagcaa aattactggt ttggtttgtt tcaggtttac
    17761 agatgagaaa agttgaggtt attccagctt gttttgcgaa tagctgctac ctttgccggt
    17821 taatattttt tttaaatgac acgtagtgtt gaaaatgtta tttcggtaaa gggtgcacgg
    17881 gttcataatc ttaaaaatat cagcctagac attccccgca acgccctggt agtaataacg
    17941 ggagtatcgg gttcgggcaa gtcatctctg gcatttgata ccatatatgc cgaaggtcag
    18001 cgtcgctatg tggaaagcct ctctgcctac gcacggcaat ttcttggacg tatggctaag
    18061 ccagaggttg atctgattag cggaatcccc ccggcaattg ccattgagca gaaggttaat
    18121 acacgaaatc cccgctctac ggttggaaca tcaaccgaaa tatacgacta ccttaagctg
    18181 ctctttgccc gaattggaag aacatactcc cccgtatcgg ggaaagaggt gaaaaagcat
    18241 tctgtcagcg atgtggttaa cttcatcagc tcctttaacg agggaaccaa aatgatgctt
    18301 ttagcacccc ttacaccgaa tggggatatg aagcagaagc tggaagctct tgctcacgaa
    18361 ggctttacac gtgttgaggt tggaaatcag atagttcgga ttgatgagca aaaattcagg
    18421 gaggtggttg acctttcggg atcggagttg cggctggtta tcgatcgttt tgccgcagcc
    18481 gctgatgatg aattccttag ccggttggcc gactcggttc agactgcctt tgcagagggg
    18541 aaaggctttt gcctgttgaa agttttcgac gacaatggga gctatagcct ggagcaattc
    18601 tccagcctct tcgagatgga cgggattaag tttgaagaac ccaccgagca cctgttcagc
    18661 tttaatagcc ctttgggtgc ttgccctact tgtgagggct atgggaaaat tgtaggaatc
    18721 gatgaaaatc tggtcatccc taaccgtagt ctttctgttt acgatgatgc aattgcctgc
    18781 tggaagggtg aaagtatgag ctggtataag gatagggttg tggaaaatgc cagccgatac
    18841 ggaataccaa ttcataaacc atactttcag ctttccgagg agcagaaatc gctactttgg
    18901 gaaggaactc ctcatttcac tggaattaat gactttttca gagagctgga gggaaaaaag
    18961 tataagatac agtatcgggt tttgctttcc cggttcatgg gaaaaactac ctgcccaacc
    19021 tgcaggggag gaagacttcg tcgtgaggca acttttgtca aggtgaacgg ctataccata
    19081 caggaattgg ttgagatgcc agtagccgac ttggcggaag ttttcaacaa tctcgagctc
    19141 accgaacatg aggttggggt ggccaaacga attcttatcg agctaaaaaa tcgcttgaac
    19201 tacctggtca acgtggggct tggatacctc acgctcaacc ggctctcctc atcactgtcg
    19261 ggaggtgaat cgcagcgtat caacctggcc acatctttgg gaagcagcct ggtaggttcg
    19321 ctgtatatcc tcgatgaacc cagcatcggg ctacatcctc gtgacaccca cctgctcatt
    19381 gatgttctga agaaactgcg taacatgggc aatacggtgg ttgttgttga gcacgatgag
    19441 gaaatcatca gggcttccga ttatatcgtt gacattggcc cctatgccgg ggttaacgga
    19501 ggcgaggttg ttttcgaagg acccatcgaa aaccttgaaa aagccactca aagcctaacg
    19561 gccaactttc ttttaagcag ggagcaaata cccgttccca gggttcggag aaaatggaat
    19621 agctatattg agatttccgg agcccgggaa aataacctaa agggtattga tgttaaattt
    19681 ccgcttagct gtctggttgt agttacaggt gtaagcggat cgggtaaatc gagcctggtg
    19741 gacgatattt tataccctgc ccttctgaag cagctaacgg gtgctggtcc ccgtgccgga
    19801 cagtttgatt cgctaggtgg cgactggaag ctaatttcag gggttgagct ggtagatcag
    19861 aatccaattg gccgatcgtc gcggagcaat ccggttacct acatcaaggc atgggatgag
    19921 attcgtaagc ttttatccga acaaccctac gcaaaacgaa atgggtataa gcccgctcat
    19981 ttctccttta atgtcgaggg tggacgatgc gaggagtgcc agggtgaagg tgttattcat
    20041 gttgagatgc agtttatggc cgatttgacc ttggtttgcg agagctgtaa ggggaaacgt
    20101 tttaaggatg atatcctgga ggtgaggtac aacggagcca acgtttatga cattttaaac
    20161 atgacggttg atcaggccat tgaatttttt agcaagggga aaactttaac tgagcagaaa
    20221 gttgtggata ggctaaaacc gcttcaggat gttgggctgg gctacataca gctgggacaa
    20281 tcgtccagca ccctcagcgg tggggaaagc cagcgggtga aacttgcctc tttccttcaa
    20341 aaggatgcgt ccattcgccc gcttcttttt atttttgacg aaccaaccac tggtcttcat
    20401 ttcaacgata ttcgccagtt gctgaatgcc tttgacgccc tgctggctaa aggacacagc
    20461 ctggtcatag ttgaacataa ccttgaagtg ataaaaagtg ccgactgggt agtagatttg
    20521 ggcccggaag gcgggcagga gggaggtttg ctggtttttg ccgggactcc cgaagatctt
    20581 gctgccaacg aggcgtccca tacgggtgta tttctaaaaa agaagctgaa tggttaataa
    20641 aaaaagccgc atgtagcggc tttgtacagt cttagctgta gctactcggc cttcaacccg
    20701 aacatgattg ctacgcaacc acttgccaac tccgagccag aatttcccga tgaaggcact
    20761 ctcaccaaaa taatcagctg ctgggttgcc tcaaccttga agttccatga tgtggcatag
    20821 ttatttttgg cattggagta tagcaccctg cggtcaacgt ccatcaccag aaattctatt
    20881 ttagccaggg attcggagcc acaaattgat attctgtatt cctggcctgc ataaaaggtt
    20941 ttaaatagct cggcgtcctc accctcggtt agaattgctg cgttgtaatt cccatcatga
    21001 acgtacggag ccaactccaa cttgcaaatt ttttttgcaa agtttttaca ttgggcttgt
    21061 gtgctgtggg gttgaagtag cagtagccct agaataagaa ttgtagtggt aaatgatttc
    21121 ataccggaat gtgttatgct atgctgtgta ctttgtacgc aattccttaa cttttgtttt
    21181 aagcttgttg aaaacatcag ggttaacggt aatgacattt tttgaacgca ggatggttgc
    21241 cccgttgttg ctagcgggaa caacttcaac ccgagatgca gaaatttgaa tgtcataaaa
    21301 tatttcgctt agcttttgca tgtcggagta aatttccata acatcagcgt tatcggaatt
    21361 ctgctttaaa aggcttaaaa cggtttcaag ggaaaacttc tgatcggcaa ttctatccac
    21421 cagctcatta tccatcttct tttcatcgct aaccagcgag gtggccaggt aaagcccctc
    21481 caaccatccg cctaccagaa ttatagatcc aatagcaacc cggtcatctt cctccaggat
    21541 ggagttggtg ttaaggaatg tttcggatat ggtttccatt attacatcgc ggttgttgat
    21601 gttttcttca agccgttgaa ttaccgagtt atcaatggca ttcagaatgc ctagcccatc
    21661 tgccattttc tttgatgcat tcatcaggtt aatcgtggcc tgggtttgat cgaacaggct
    21721 ggcgtagctt aagtcggtgc tgtagattcc caggtttaac gccatgcttt tactggtgtt
    21781 gtagtgcgca acgttttcaa ccgggtttag caggtcttca ttgtatactg cgcctgaccg
    21841 ttttagtatt agggccgttt cgagtgggga gggcagggag tagaatattt gcttaaccga
    21901 ttggatgctt tgagaaatca cgctatcagc ggcaagttca tcctccgtga cttttaacga
    21961 tttgtccgag ggtttctccg atttgcaggc taccccgcct actatcagca gcagtcctaa
    22021 taactttact attcgcttga cgaaacctgt agccatatca ccaactttta attgtcaatt
    22081 ttacccacat tttcagtaag taaagataaa ataatttttt actatattcc tatagaaatt
    22141 cattgttgaa ttcaaataat tgcaaaaaag ttgataacca gggttgattt tcaaccattt
    22201 tgttgtttgg atggaatcaa gctatgtcgg tccagtaaat gcaacatgca gaaaaataat
    22261 tcctctgctt aaggcttaaa aaatttcggc tacctccttt tttaggaaat caatagcaag
    22321 cgtagtgggg ttagtatcaa gctccacgta ggtttcaatt attttttggc tcaaggccat
    22381 gctggtttcg ttggggttta tcctgtcggc acaaacgttg ataagcttaa taatgttctc
    22441 cttggcattt ttaaccatct cccaggcttc cttgcttacg tagagctgct gcgataggtt
    22501 gtgctcccat tcagccctta tggtgcccag caatgccgtg tgcaggtcgg aagccagcat
    22561 cccttttttt gtagttcttg gcagcaacga ttcgggtgat attcgttcga ggaaaagcac
    22621 aagcctctcg taggcttgca gcctcactgg ggtggttatc tttatgttat taaacatcag
    22681 ctcagatttc ctgcggttgg tttcgctctt aatcatggcc gatatggcaa ggtatcccgc
    22741 cagaaaaacc attagagcgg gcagcactat cttcagaatt tctgctagca tgggttagga
    22801 ttttaaggga aagtttgact atttttgagt tcttaaaatt acaaaaagaa ttttgaattt
    22861 attttaaatc tatacggtta tggagaaagt atcttctcga atagcagcat tggaggaatc
    22921 tcaaaccatt gccatgtcgc aaaaatcacg ggagttggcg gcacagggaa ttgatgtggt
    22981 taacctcagc ttgggtgaac ccgatttttt tactcccgat tttattaagg aggccgcaaa
    23041 gcgcgctatt gatgagaact tttcgtttta ccccccggtt tcggggtaca aggacttgct
    23101 ggaggcgata tgcggaaagc tgaagcggga gaatgatctt acctttaccc ccgaccaggt
    23161 tgttgtttcg gctggtgcta agcatagcct gtcaaatgtg ctaatgtgcg ttgttgaaaa
    23221 ggatgatgaa gtggttgtcc cagcacccta ctgggttagc tacgttgagc ttattaagct
    23281 ggccgaaggg cggagcgtta tagttccaac caccattgag aatgatttta agattacccc
    23341 tcagcagctt gaggcagcaa taactccaaa aaccagggca tttctctttt gctccccttc
    23401 aaaccccaca ggaagcgttt actccaaggc tgaacttgag gccattgcca gggttattga
    23461 gcagcaccca aatatcatag taatttccga tgaaatttac gagcatatca acttcgtggg
    23521 aaaacatgag agcattgccc agtttgatag cattcgcgac agggtggtca tcattaacgg
    23581 ggtgtcgaaa ggctacgcca tgaccggttg gcggattgga ttttctgcgt cggctaaatg
    23641 gatagccaag gctgctgcca agctacaggg acaacaaact tcgggagtat gcaccattgc
    23701 ccaaaaagct gccactgctg cctttaactc cgatggtcaa accacccgcc aaatgtgcga
    23761 ggcgtttaaa agacgccgcg acctgtttgt aaagggaatt tcgggtatca acggtttcaa
    23821 aataaatgtt cctcagggcg cattctacct cttccccgac attacgcact tcctgggttt
    23881 gcaatttaat gagcaggttg ttgctactgc tgacgatttt gccatgtatc tgctaaacaa
    23941 cgctcatgtt gctgttgtat cgggctcggc ttttggttcg ccctacaata ttcgtttgtc
    24001 ctatgctacg tctgacgaaa aacttagcat ggctgttgag cgcattgccc aggctgttaa
    24061 gctactacgc tagcaacttg ttgagcttat tcaacctaaa actgatttaa cttgagccaa
    24121 ccccagctaa agactttttc cagaagatgg ctgcctatag ttttggcatt agcaggattg
    24181 tttgtgggct ttttggtgct ttaccgctat gttactactt tccgtgtcga caatgttgcg
    24241 ttaacgcacg atacatactt gtacataccc accggatccg attttgatga agttgttagg
    24301 gacatggaag gtaagggtct cctaaaaaac gttggtagct ttataaaagt ctccaggaag
    24361 atgggttacg acctgcatgt taagccagga cggtaccgtg taactcctga catgagcaac
    24421 caaaagctgg tgaacaagct tttaatggga gcgcaggacc cagtgagggt tacatttcac
    24481 aatatcagaa ccaagcagca gctctcggga cgaatttcca aacagctgga ggctgattcg
    24541 ctttccattt tgaccatgct gaggtcgcag gttgatgcca tggcattcgg atttgatact
    24601 gcaaccataa tggccatgtt tattccaaac acctatgaat tctactggaa taccgatgcc
    24661 aacgactttt ttacaaggat gaaaaaggag tatgaaagtt tctggacacc tgaacgcgag
    24721 cagaaagcct atcggcttgg gataacaagg gtagatgttt ctatattggc ctcaattgtt
    24781 gaggaggaaa ccagaatggt aactgaaatg cctgttattg caggagttta cattaatagg
    24841 ctgcataggg gaatccccct tcaagccgat ccaaccgtta ggtttgccct tggcgatttt
    24901 tccatcagaa gaattctttc caaacacctt caactcgatt cgccctataa cacgtacaag
    24961 tacaagggac ttccaccagg tccaattaga tgtccctcca ttagagctat tgacgctgtg
    25021 ctaaatgccg agaaacatag ctatctctac ttttgtgcaa agcctgattt ttcgggctac
    25081 catgtttttg cccgaactct catggagcat aaccggaatg cagaggcata ccgcagggca
    25141 ttgaataggg aaaaaatttt caggtagctt tgctgccaga aatctccatg cgtttgctaa
    25201 cttgcatcca tgtttctaat tctaaaacct acagcaatga aacagctaat tacaccaatt
    25261 cttgtatgcc ttagcggaat cctcctggcc caacctgccg aggttttaac cttaaataaa
    25321 ccctcattat ccagagggaa aaacattatg gaggccctag ccagcagagc ttccgccacc
    25381 gaatttacat ccgatgagtt atccctgcag gatatttccg atttgctttg ggctgcaaac
    25441 ggcattaacc gacccgaaat tgggaaaaaa acggcacctt ctgcaacaaa ctctcaggat
    25501 atagacgttt atgtggttac ttcaaaagga gcctatatct atgaacctgt acagcatcag
    25561 ctaaagctgg ttaatgctgg agattttagg gagttagtgg ctggccgtca gaagaacttt
    25621 gccaacgctc cgctattctg tattatggtt tcggatatct cacgattccg gggtaatgat
    25681 gattctcaga aaatgaggtg ggctgcaatg gatgctgcca ttgtttcgca aaacattaac
    25741 atattttgct ctggggtggg cttgagaaca agaaccaggg cttttatgga agcagataag
    25801 ctacgtgagg cgctaaaact taccccctca cagttcccaa tgctaaataa tccggtgagc
    25861 aattaggcat attgtagagt tcctaacgca attttgcttg tttaccatct ggaattttat
    25921 aatactgaca ttactgaacc ctgatgtgcg ggaaactttc agcagataaa aacttatcat
    25981 caaatcagca accagaacaa gttatgtttt gagcacttgc tgcaactttt ttaattttga
    26041 gcagggtaaa agtttacact atacatggtt aaatttgcaa gcagttctat gtttcgtttc
    26101 gtaaagatac ttctaaccct ggttgctgtt ttactggctg agattacttt tgcccagaaa
    26161 ttctttgagg tcgaaaaagt tgaggatgcc caggttaaat tttatgcggt tgatgacccc
    26221 aaggatgccg atctgctgtt ctgcattatc tatgaggaaa aattgattac taaggccggc
    26281 attatgatgg aggtggaaac gcccagggag gcgcaagtgc tcattatttt tgttgacgat
    26341 ccggctcttg ccgacattaa ggtttggctg gttgaaaaac cggaggaggt ggtgtggaaa
    26401 aacgaatcga aacgaaagtt gctttctgtt ggtgggctca agtaatgatg ggggctactt
    26461 ttttttaatt tgaagtaata gcggtgcagg taatctcgaa ccataaacta tcaatttcct
    26521 atatgaacga tcaacaccga tacaaccaac ggggagtttc agcatcaaag gaggatgttc
    26581 ataacgcaat aaaaaatctg gataagggcc tataccctaa ggctttttgt aaggttgttc
    26641 ccgatatttt agctggcaat ccggattact gcctggtgat gcatgccgat ggggcaggaa
    26701 caaagtcgtc gttggcctat gcatactggc gcgagactaa tgatatgtcg gtttggaaag
    26761 gaatagctca ggatgctgtt gtgatgaatt tagacgacct gctttgtgtg ggcgttacgg
    26821 ataacatact tctctcctct actattggcc ggaataagaa ccttattccc ggcgaagtca
    26881 tttctgcgat agttgaggga accgctcaat tttgcgagga gatgcaaaaa tatggtatcc
    26941 acataacgct tacgggtgga gaaacagccg acgtaggcga cttggtcaga accgtaattg
    27001 ttgactctac cgttattgcc aggctgaagc gagcggaggt aatatctaac agtaacattc
    27061 aggatggaga tgttatcatc ggcctatcat cgtgtggcaa ggctaattat gaacaggagt
    27121 acaacggagg catgggtagc aacgggctca cctctgcccg ccatgatgtt tttagcagct
    27181 acctggccga taagtatccc gaaacattcg atccgtcagt tcctcgcgag ttggtgtaca
    27241 gtggtagctt taagctcacc cagatggttg atgaagtcgg aattactgcc ggaaagctga
    27301 tgctttcacc tacaagaacc tatgcccctg ttgtgaaaga ggttcttgac agctaccgtc
    27361 ctgtgattca tggccttgtg cattgctcgg gaggagctca agctaaagtg ctcaacttcg
    27421 ttgataacct acacgtggtt aaggataacc tttttcccat tcctcctttg tttaggatta
    27481 tccaaagcga gtcgaataca tcgtggaggg aaatgtacaa agttttcaac atggggcacc
    27541 gatttgaaat atacgttttc cctgaatttg ccgatgatat tattgccatt tctaaggatt
    27601 ttggcgtaga tgcaagggtt gttggctact gtagcagctt tcaggggaaa aagctaacaa
    27661 ttaaatcaga aaaaggtgaa ttttactact agaaagtggt gtttgagctc ttttgaacgt
    27721 gaagaataaa attttgtata gccctctccg tgcttgctgt tattagtcat tcaatcagct
    27781 gtttaccaca ttattaaaaa ggtgtattat accctgcacc aaagatctga acgtcacagt
    27841 aatatcaacc ttgcaccttg ttgggtaaag cattatgagt ggtggaagaa tactcgcttt
    27901 ctaaatttat gcttaccttt gcgcattata ttaccagtct atgtccgaca atacaattac
    27961 caggaagcta gaaggagtaa ggcaaaagtt cgaagaaatt gggcagaaaa taaccgatcc
    28021 ggccgtaatg gccgatatga aaaaatttgt aagcctgaat aaggagtaca aagcgctaga
    28081 acccttagtt gctgcctacg aaaagtacaa gaatatactg agcaacatcg aatcgtcccg
    28141 gcaaatactt agctccgaaa aggatgagga gctgcgagaa atggccaagg aggaactcga
    28201 tgcccttacc caggagcagg aaaagctgga ggaagagata aaattattac tgctcccggc
    28261 tgaccctgaa gactccaaga atgccgtgat ggagatacgc gccggaacag gtggcgacga
    28321 ggccagtatt tttgccggcg accttttccg catgtacacc aagttttttg aacggaaggg
    28381 ttggaagtac gaggttacca gctcttcgga gggtacggtt ggtggttaca aggagattgt
    28441 gatgagtgtt accggaaacg gcgtatatgg cattttgaag tacgaatcgg gtgtacatcg
    28501 ggtgcaaaga gtcccacaaa ccgaaactca gggaagaatt cacacatcag cagccactgt
    28561 tgctgtgctt cccgaggctg aagagttcga tattgacctt aaaatggagg atatccgaaa
    28621 ggatattttt tgctcttcgg gtccgggtgg tcaaagcgtt aatacaacct attccgccat
    28681 tcgtttaacc catattccca gtggaattgt tgttcaatgc caggaccaaa aatcacagct
    28741 taaaaacttt gacaaggcgt tggccgagct acgtacccgc ttgtataacc ttgagtacca
    28801 aaagtacctc gacaaccttt cgtccaagcg taaaaccatg gtttcaaccg gcgaccgatc
    28861 ggctaaaatc cgaacctaca actacccgca gggacgagtt accgatcata ggataaacct
    28921 taccctttac aacctctctg ccatcatgga tggagatatt caggagctaa ttgacaagct
    28981 tcagctagct gagaatgccg aaaggctaaa agccgaatca acagactaac cagtagccag
    29041 aatgactccc gaacagctat tcgaccagat aagattgaaa cggagtttcc tatgtgtggg
    29101 gttggacacg gagcttacaa aaattcccga gttcctcctt accaaggatg acccgatttt
    29161 tgagtttaac aaggcaattg ttgatgccac tcacgatttg gccattgcct ataagctgaa
    29221 tctggcgttc tacgaggtgc atggcgctac cggatggaat agcctggtta aaaccatcaa
    29281 gtacattcga cgcttctacc cctcaatgtt catcattgcc gatgccaaac ggggcgatat
    29341 tgccagcact gcacgcatgt atgcaaatgc ctactttaaa agtctggatt gtgatgcggt
    29401 taccgtttcg ccatacctgg gtcacgattc cgttacgcca tacctgaatt ttgagggcaa
    29461 gtggactatc ctgttggcca tcacctccaa cgatggcttt cgcgatttcc agctggccgt
    29521 taacagggat acaggtaact ttatgtttga ggatgttgtt gccaagtcgt tgcagtgggg
    29581 caacgaggct aatataatgt acgtggttgg ggcaactcat cccgagtatt tcgccgctgt
    29641 acgaaaaatt gttcctaacc attttctgct tgtcccgggt gttggggaac agggtggtag
    29701 cctcgaaaag gttgccgaac atggtttaaa taaccgctgc gggttaattg ttaattcttc
    29761 aaggggcatt atttatgctg atattaccag ccgatttacg ggcgcagcta gagcgcgtgc
    29821 cctaaagctg cagcagcaga tggaatctat tttaactgcg gcaaaacttg tttagccctt
    29881 tttcatttta cacgttggta gatattcaat gctctacagc cacaatacgg attttttact
    29941 ttacaagcag ggccgaaaac catctccaga actccctgtg aaaattttca ccggattccc
    30001 caggataaag gttcaagtac gattggcctg actgttggtt tggggtaatc aaaaacagct
    30061 cattcctgtc gtacagaagt gatgcctcgg ggtgaaactg aacaccaacc acgtttttat
    30121 acttgatatg gcgtattgcc tccaccactt taccgtcagc tgaatatcct gctaccctaa
    30181 tgttgtcgcc tagcattttc aaacactggt ggtggctact cagcacaaag ggctttttac
    30241 cggcagctat tgagctgaac agctcatccg aaaactctac ctgatgaaaa gttccaccga
    30301 gaagctgttc ttcgtaacca tagctgcagt tgtagttacg gtgctgctgg ttgatttcct
    30361 gtgccagcac cgattcaaca gttgaaaggt ggtagatatc ggttggaata tcctggtaaa
    30421 gcgttcctcc ggctgccacg ttaatgctct gcattcccag gcaaattccc agcacagcaa
    30481 ggtttgagct atcagccagc agcggcttaa aaagggtatc acgccttcca cccagtaagt
    30541 ggaagaggaa ggaaatttcc atgctatgcc tttccgggtc ggttatttgg gttagcaggc
    30601 tggtgggctc attatatatt gaaggcggta tatcgggacc tccggtaaaa attataccag
    30661 cacatgtgct tactagcttc tcaaatattc cgctacaggc gttacggtga aatatgctgt
    30721 ccggtgtgat ttcgggtttt atgggcagaa gataaaagta gtcaatggcg ttctcagcaa
    30781 tgtactgctg cgagagggca taatcgtaat tttctccact gtggtaaatt ccaacaattc
    30841 ttgttctgtt tggcagtaca aggaggccgt ttttcaccag cttgtcgaat gttttgatgg
    30901 tgctaacggt tgggtgggtg ataataagcg atttaccatc ctttgagcaa agctcttcaa
    30961 ctccaatggt aatttcttct gaactgcgtg tattggtaga gcaaccggtg tgtagcacac
    31021 tggttgtgat gagcattgat gctgcaagca gccggatttg agctatgcgc atagttttcc
    31081 gattttaata ggcagaatta aatctattcg actcattaaa catcg
//
LOCUS       DAOSHM010000029        30039 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_1194_length_30039_cov_21.055696, whole genome shotgun
            sequence.
ACCESSION   DAOSHM010000029 DAOSHM010000000
VERSION     DAOSHM010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 30039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30039
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1194_length_30039_cov_21.055696"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(388..1227)
                     /locus_tag="PK839_06135"
     CDS             complement(388..1227)
                     /locus_tag="PK839_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HOG72352.1"
                     /translation="MEDSEKIKEFVRQKYSEIALQDKETNQSSCCGSGCCSTEVYNIM
                     TEDYSTVEGYNPNADLGLGCGLPTKFAGIKKGDTVIDLGSGAGNDCFVARNETGADGR
                     VIGIDFTPAMIEKAKANASKLGFANVEFRLGDIERMPVSANMADVVISNCVLNLVPNK
                     DRVFNEIFRVLKPGGHFSISDVVLVGNLPDELCNAAEMYAGCVAGAIQKDHYIELIQK
                     NGFTNIAIQKEKPITIPDDILKSYLDEAALQNFKSGTTGIFSITVYAEKPAACGCGTG
                     CCS"
     gene            complement(1239..1574)
                     /locus_tag="PK839_06140"
     CDS             complement(1239..1574)
                     /locus_tag="PK839_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002978307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOG72353.1"
                     /translation="MGVTKTEHYAEQQKAISELAKAMGHPARIAIVEHLVKVNACICG
                     DIVNELPLAQPTVSQHLKILKEAGLIKGEIEGNSICYCINQDAINRLQKYFDDLSAKL
                     NFKSNNCCK"
     gene            complement(1818..3203)
                     /locus_tag="PK839_06145"
     CDS             complement(1818..3203)
                     /locus_tag="PK839_06145"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOG72354.1"
                     /translation="MNKRLLLLMLPVLAACGTKGPKEAVIAGKFTNSPDSNVYISTAG
                     ITDTVALDENGGFVYRAKLDKPQLYKLNIARTQTMVYLNPGDSSYMEFDVTSPVDGPV
                     FSADLKNIHQSIFSRRAALHNLMGSVNTLYSLSYDNFNRKLDSIQQVLYSMIDSASIE
                     PKDLAELEKTRTRYVILNVKANYPEYSAYLGGKEFNPDSVDYSFLSEVDPNNAYHLMF
                     DEYTSLVSIITQNQLVKKPGYKELLAKSSGERLPFEFKLIDSMLANPKVRDYLKMNKL
                     INDIEYGEFYELEGVVKSYMDSCQTPAYKQSVGNIFNQKMQLAPGKPAPVFVCKDING
                     KEYSLEDFRGKLVYIDFWATWCGPCRHELPYLEELQKDFKGKNIVFVSISLDDNKAAW
                     EKMVKEKQMKGVQLYGEDAWKSIPATSYQIKGIPTFFLIDAKGNIIKPNAPRPSSNEI
                     RPLITAELEKI"
     gene            complement(3687..5411)
                     /locus_tag="PK839_06150"
     CDS             complement(3687..5411)
                     /locus_tag="PK839_06150"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOG72355.1"
                     /translation="MRTLNRFNKLILLVAALNYHQAAAQNGILKSPLDLKPALSGSFG
                     ELRTAHFHSGIDLRTNGKIGFRVYASEKGYVSRIKVSPVGFGKVIYITHPNGLTTVYA
                     HLDRFTNAVAEYVEKQQYASHSFEVELFPKPGEFNVSKGEVIGFSGNSGSSGGPHLHF
                     EVRSSATQWPVSPLKYLDGNFLADNSAPTIKSAWFYEIDSMDYLNDSILPNPISIKKT
                     GNLYFAPDTIRIAGKSRMGFGVEAFDIINGSSTTCGFHKLSMLVNNKQTFSFAVDSFS
                     YAETRCLNSIIDYSIRQLRQQEIVRLWADPGNLFSGLKADKSLGILTVESDSIYKITI
                     ELEDFYKNTSKLEMVVKGVHPFKNPESTPGLLRHSYVSWWNSYSFDSKDYSIYIPRRT
                     LYHDILLRHSALHTDSLAFPVISIHHARTPLNGKFTLRVKIAGVPANLRDKCFIGYIN
                     GKGIEYCSSRQDGPDMVTSPDKFGKFTLALDTVPPRIIPLNISQNADIAGQSSIKIKL
                     TDNYGIGTYSGFIDDGWVLFEWDPKTSLLTHTLTPKRERQNTWHTLRVEVADLLNNRV
                     TYKCRFYW"
     gene            5694..7937
                     /gene="purL"
                     /locus_tag="PK839_06155"
     CDS             5694..7937
                     /gene="purL"
                     /locus_tag="PK839_06155"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF002290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HOG72356.1"
                     /translation="MIEHQAMQQHEVTMDTVRELGLLPEEFEMIKKFLGRVPNYTELS
                     IYSVMWSEHASYKNSIKWIKTLPRKGSALLAEAGAENAGLVDIGGGYACAFKIESHNH
                     PCAVEPYQGAATGVGGINRDIFTMGARPVAQLNSLRFGNPKLERTRWLMRGVVKGIGD
                     YGNAFGIPVLAGEVFFDDSFNTNPLVNAMSVGIVSKDSVVSAVAKGVGNPVYIVGSAT
                     GKDGIHGATFASANITENSADDIPSIQVGDPFQEKILLEATQEVIKTGALVGMQDMGA
                     AGIICSTSEMSEKGKHGMRIDLDKVPLRQTNMEAWEILLSESQERMLMVVKKGRERDV
                     EEILNKWDIGYAVIGNVTSGDSLEFYYHNELAAKVPASSLVLGGGAPVYDREYSEPEY
                     YKEFKKFNMNQIPEPADLVEVARFLTAHPNIASKRWVYQQYDTMIGTGNMTTNFPSDA
                     GVFNLKELNKALAMTVDCNSRYVKADPEVGTMIAVAEAARNIVCSGATPLAITDCMNF
                     GNPYNPEVYWQFVKAIKGMAKACKKFDTPVTGGNVSFYNQSSINGREEAVYPTPTIGM
                     IGMLENKNHHTTLAFEEKGHMIFLIGKSRNDIASSEYLYSYHGIKQSPAPFFDLDEEF
                     AIQQALLDLIARNLVQSAHDVSDGGLFIALLESAMPRNLGFDITTDAEIRTDAFLFGE
                     SQGRIVVSVSPSRETHFIDYMVEHGIPFTALGHVTKSEIRIDDVSFGFVADLKQLYDT
                     ALEKIMA"
     gene            7953..8663
                     /gene="ubiE"
                     /locus_tag="PK839_06160"
     CDS             7953..8663
                     /gene="ubiE"
                     /locus_tag="PK839_06160"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009126981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HOG72357.1"
                     /translation="MPTPDKSSQNVEAMFNSIASNYDFLNHFLSLGIDRKWRKRLIRL
                     VAKGNPQKVLDVATGTADLAMLLAREYGGVSITGVDISENMLAIGKRKVESENLSGQI
                     SLHRASALNLPFENDSFDAAMVAFGVRNFEDLSMGLSEVYRILINGGQFAILEFTIPD
                     KFPVAQLYRFYFRHVLPLFGGIISGSRTAYKYLPESVLAFPEKENFLEIMRQVGFTGC
                     TCKSLTFGIASIYTGFKP"
     gene            8917..9657
                     /locus_tag="PK839_06165"
     CDS             8917..9657
                     /locus_tag="PK839_06165"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOG72358.1"
                     /translation="MKKIFVVIAVIWCSVTAAQAQYRKPNILNDPDYDVGKPLRFGFS
                     IGINIMDFDIKNSSVPELNADSSQFQYFTEVTHINPGLNVNAISDLRLAENFHLRFLP
                     GYSFGQRNLDFFKVENGRVTHETTMDIESNFIDLPLGIKYLAERTSNIRPYLYVGGDY
                     RIDLAAYKRLKVEKGILVRLQKQDFYYELGFGVDFFFEYFKFSTEVKWSAGIGNVISS
                     DYAEGAENYRYAVDAMNSRLIMISFHFE"
     gene            9853..11412
                     /locus_tag="PK839_06170"
     CDS             9853..11412
                     /locus_tag="PK839_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HOG72359.1"
                     /translation="MAKDIILELTPENAANSQLLLKAVASKLGMSVKDVGHYTITRRS
                     IDSRHGKVRVNLGIRVFDRDEPIADGKPQFTYGDVSKSPPVAVIGAGPAGLFAALRLI
                     EHGYKPIVVERGKEVSARKRDVALLNREHKLNPDSNYAFGEGGAGTFSDGKLYTRSRK
                     RGDYNRVLYILCNHGASEDILFDSHPHIGTNRLPGIIVKIRETIISYGGEILYNSRVE
                     SIKTDGSRVTGVKLSDGRLVEAKACILATGHSARDVYSMLLNQQIQIEAKPFAMGVRV
                     EHPQAIIDSIQYHGTPRGDYLPAASYSLVQQVEGRGVYSFCMCPGGFIVPAATEPNEV
                     VVNGMSPSLRNSRWANSGIVVEVRLDDLKSYSKYGELAGVEYQHYLEELASLHGGFGV
                     KAPAQRLVDFVDGNYSASLPSCSYHPGITSSELHAWLPDDIGFRLRKGFRAFGARMKG
                     FLTNEAVVLGVESRTSSPVRIPRNPETLQHVGVEGLFPAGEGAGYAGGIVSSAFDGER
                     CADAVAFYLGG"
     gene            11656..12231
                     /locus_tag="PK839_06175"
     CDS             11656..12231
                     /locus_tag="PK839_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HOG72360.1"
                     /translation="MGRKIVVGVTGASGAIYARSLLEILGELSDQLEEVALIFSDNGR
                     AVWEFELGHIPPFSAPFRVYDNNNLFAPVASGSAGFDTMFIAPCTMGTLGRIAAGTSS
                     DLICRSADVMLKERRRLIVVPRELPLSLIHIENMRTVTLAGGIIVPASPSFYSRPSSM
                     EELVRTVVFRMIDLAGLELPHYRWGEAKSDS"
     gene            12257..12907
                     /locus_tag="PK839_06180"
     CDS             12257..12907
                     /locus_tag="PK839_06180"
                     /inference="COORDINATES: protein motif:HMM:NF016232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG72361.1"
                     /translation="MQGEEKVVSRYITNLIGMGEGLTLDFKHSITDSKKIARSLAAFA
                     NTQGGTLLVGVKDNGSITGVDTDEEYYMVEAASTLYCRPEVPFEVVKWNMNAKTVLEV
                     KVKPSKLRPHKAPDKNGHYKAYIRIADENMVVTSTQLKIWKAERSRKSIGLNLSNAQT
                     ALFEHFKQNPRITIDEFCKIGFLPATEAERILVNLTVLGTIRYSGNPADEYFYPSV"
     gene            13280..14152
                     /gene="lipA"
                     /locus_tag="PK839_06185"
     CDS             13280..14152
                     /gene="lipA"
                     /locus_tag="PK839_06185"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009123058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HOG72362.1"
                     /translation="MTEVRKPAWLKVKLPGGEGYRRVDSIVKNNSLHTICSSGMCPNI
                     GECWGNGVATFMILGDICTRACKFCATTTGRPLPPNPNEPEKIARSIQLMALNYCVIT
                     SVDRDDLPDQGAAHWRNTVNAIRRVNPNTMIEVLIPDFDGRSDLIDIFLESNPNVVGH
                     NLETVERLTPEVRTKARYRTSLETLSYIASRGFTTKSGIMLGIGETFDEVVQTLDDLS
                     AVGCRMITIGQYLQPRPNNLPVQRYVTPEEFEEYARIARQKGFVFAEAGPMVRSSYHA
                     DKAQKLFGTRPDSV"
     gene            14192..14929
                     /gene="lipB"
                     /locus_tag="PK839_06190"
     CDS             14192..14929
                     /gene="lipB"
                     /locus_tag="PK839_06190"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HOG72363.1"
                     /translation="MQNVIFTDLGTIDYKEAWDLQEKLFDEVSLNKAEGDGNLNFLLF
                     CEHPHVYTLGKSGQRSNLLVSDEMLKRINASFYHINRGGDITYHGPGQLVAYPIFNLE
                     AFGLSLKSYISLIEQVVIDCLGDFGVPANRLDGATGVWLDAGIKGKERKICAIGVRAS
                     RFVTMHGLAFNINTNLDYFGYINPCGFTDKGVTSLQRELGEEVNPEMVKQRILDHFAR
                     KFNFTVNTRLFPNLKLATNYEGGKHNG"
     gene            14904..16646
                     /gene="recJ"
                     /locus_tag="PK839_06195"
     CDS             14904..16646
                     /gene="recJ"
                     /locus_tag="PK839_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HOG72364.1"
                     /translation="MREGSTMDKRWVFKDVDGDEAVNKLAAELNIPHTLASLLVQRGI
                     KTFDEAKRFFRPSLDDLYDPFLMKDMKEAVDRIVTAIHHGESILFYGDYDVDGTTAVA
                     LLYSFFRQRYSKIGYYIPDRYGEGYGISYKGIDYAFENGYSLIIALDCGIKANEKVLY
                     ASQRKIDFIICDHHFPGDQVPGAVAVLDPQRPDCHYPYKELSGCGVGFKLLQGYCLKT
                     GTPMQELFQHIDLVAVSIASDIVPITDENRVLAYYGLEKINSNPSKGLKTIIQIAGID
                     KQKIAIDDIVFRIGPRINAAGRMESGKSAVDLLCAADDGTAYEMGSLVNSCNNDRKNV
                     DRSITHEALRMIAADARLQHCHSTVLFNPSWHKGVVGIVASRLIETYYRPTVVLTQSG
                     DLATGSARSVPGYDLYQAVEACSDLLENFGGHMYAAGLSLKLENVENFKNRFEEYVSQ
                     TITPELMMPQIEIDARIKLSDINDKFYRILKQFQPFGPGNMSPVFAAENVRDNGECRL
                     VGPDKEHLKLSLAEEGNPNIYHGIAFQLGEYYRDIHNGKSFNVCFNIYENVYRGITTL
                     QLRVKDLKFIGNQD"
     gene            16807..17826
                     /locus_tag="PK839_06200"
     CDS             16807..17826
                     /locus_tag="PK839_06200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72365.1"
                     /translation="MKARYLIIGAVLLPVASYGQKQLQPTIAAMKWGVDYKLHVRLAN
                     DSAYVVDVRGLYHTGRDLFVDTTDNSATYYPVTLDREFVDYIKSKKIDTSDSLSLADS
                     NRTASYKTLWSALHSNLGGGYIHFVNCLIYALESQNLKLNDPIFKRPATRWKPNPVTQ
                     SYKRTHKWSYFYPDNQKLAQKEYRIRLKENDLKDLQGVPLQFTNLFLHTSQKKYEELV
                     RQQKNREVAQINLIRLLLGAKYLGEDQIRHISNSVISAVLKYNVNTLPSIIIFDDYNA
                     AVALTLDMNGYRIDRIVYSDGEVISGKELEGRTAIIDGLIRNINEANERVFQKKLQLY
                     YQKTR"
     gene            complement(17998..19671)
                     /gene="ettA"
                     /locus_tag="PK839_06205"
     CDS             complement(17998..19671)
                     /gene="ettA"
                     /locus_tag="PK839_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HOG72366.1"
                     /translation="MADEKIIFSMVGVCKTFPPQKKVLNNIYLSFFYGAKIGIIGLNG
                     SGKSTLLRIIAGVEKSFDGEVVFSPGYSIGYLEQEPHLDESKTVKEVVQEGVAGTVKL
                     LEEYEELNARFAEPMDDEQMNRLIQRQGELTELIDHADAWNLDSKLERAMDALRCPEG
                     STPVKILSGGERRRVALCRLLLQEPDILLLDEPTNHLDAESVQWLETHLQQYKGTVIA
                     ITHDRYFLDNLAGWILELDRGEGIPWKGNYSSWLEQKTQRLAQEEKQESKRRKTLERE
                     LEWVRMSPKARQAKSKARLSAYDKLLNEDVKQKEEKLEIFIPNGPRLGNLVIEAKDVA
                     KSYGEKLLFEHLNFILPPNGIVGVIGPNGAGKTTLFRLIMGMETPDYGEFRIGDSVKL
                     AYVDQQHQAIDPEKTVFEVISGGLDSMMLGSRTINARAYVARFNFSGADQEKKCGMLS
                     GGERNRLHLALALKEEGNVLLLDEPTNDIDVNTLRALEEGLEDFAGCAVVISHDRWFL
                     DRIATHILAFEGDSQVYFFEGSYTDYEENKRKRLGDEGPQRIKYRKLKE"
     gene            complement(19748..20593)
                     /locus_tag="PK839_06210"
     CDS             complement(19748..20593)
                     /locus_tag="PK839_06210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72367.1"
                     /translation="MKKFAVTLAILFGVANLYAQRNLVRPEDVKTFMASKTYIVLEEN
                     PTSEYNIEIREAVERSWKITPYEFITSKEFEKVRQDIDKSFLVLLQVRFDNDKVAPVY
                     NFLSVSLGAAVKKLTDMPDICSVPLSYKEVSEDTYTYKLEGLINFMQNYIRDINTNPK
                     LIKKDAFKLYNKNMKQIKNKELWVTESSLDKTTRALTDIRNNYKYSIKVVKPEDIQKA
                     ILERNPNVVFLHKVGPEGTRLQWRVWKMIIGAGDGMMYYYDMHTISKSAGDGFLVKDF
                     KKIGS"
     gene            21011..22831
                     /locus_tag="PK839_06215"
     CDS             21011..22831
                     /locus_tag="PK839_06215"
                     /inference="COORDINATES: protein motif:HMM:NF039989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOG72368.1"
                     /translation="MEKKKIVDVHEVKKVVNLGPIVGDGVAHLIMSALRFNKLNEKYS
                     NISHLKGLEFLSAAMDTFEITYEVNPEELKRIPRTGAFISVSNHPFGGVDGMILIKMM
                     LEQQRTDIKILSNFIIQRIPQIAEFVFPVDPFDNGVKRGSNLIGLKKALTHVSNGGAL
                     GLFPAGEVSTYYDNMDEVGVVDRQWQPSMIKFIKRAGVPVVPIYFQGENSRIFHLLGL
                     IHPMLRTAKLPSELLNKKNQVIRVRIGYPISVEEQDAYCDLDRYGRFLRVKTYALGSS
                     LDVDKFLSYKLRSAPVPEPVIPPVPFSAVENEVEKLLSKYLLFRSKNYAVVCAPSVEM
                     PNLMTEIGRLREITFREVGEGTNRKIDVDEFDLYYWQLFIWDEEEKRIVGAYRAGKGK
                     EIIENYGIEGFYIQTLFKIDKEFAPILEQCIELGRSFIVPDYQRKPLPLFLLWKGILY
                     FLIKNPEYRYLIGPVSISSRFSDYSKGVIITFMKSNYFDHNFARFIRPRNSFRVPVKE
                     EEFKIIFDTSDDLDKLDKFIKEVEPYDYRMPVLLKKYLKLNGRILGFNVDPKFNNALD
                     GLLILDLLQVPIQTITSLSKEINDKSILERFNISDYTFED"
     gene            23116..24354
                     /locus_tag="PK839_06220"
     CDS             23116..24354
                     /locus_tag="PK839_06220"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HOG72369.1"
                     /translation="MRLALYFLLAAIFALSCNRQVVSEPELVERANELHKKIVTIDTH
                     TDAPLTFMRTGYDFSGKNNKAIGSCVDLAKMEAGGLDAAFFAVFISQDECTPDAYRKV
                     NAQALQIASTVINTVAKYPDRAEIAVNPEDIFRLKAAGKRAIYIGMENGYPLGEDLSQ
                     LKVFYDLGARYVTLCHTRNNQICDSSTDPEGSRNNGLSDFGRSVVAEMNRLGMLVDVS
                     HISDKSFYDVLAVSKVPVFASHSCARAICNNPRNLDDNMLKALAKNGGVVQMCILSDY
                     VKQGEKNPVRDSAFRALRRKYGNFRNLTPEQEEQAEADWHNLEQQYPAKLATVSDVVD
                     HIDHIVRVAGIDHVGIGTDFDGGGGVDGCRDASELHAITIELLRRGYSERDIRKIWGE
                     NFLRVFRQARDYAAAHGQGK"
     gene            24716..25348
                     /gene="yihA"
                     /locus_tag="PK839_06225"
     CDS             24716..25348
                     /gene="yihA"
                     /locus_tag="PK839_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009346821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HOG72370.1"
                     /translation="MVIRSAKFVASYPGVDRCPASSIPEYAFIGRSNVGKSSLINMLT
                     GTKTLAKISETPGKTQLINYFLVNESWYLVDLPGYGYAKASKDKRKEFSQIITNYILK
                     RSSLTLLFVLIDSRLEPQPIDIDFINWLGVNQIPLALVFTKTDKTPKALLEANMEAFK
                     NKMMEMWEVLPPIFTSSAVTRTGREDVLSYIDSINANFKSTQPKKVERVW"
     gene            25391..26335
                     /locus_tag="PK839_06230"
     CDS             25391..26335
                     /locus_tag="PK839_06230"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HOG72371.1"
                     /translation="MHSKHNIKFFTGRSSRYLSERIADSFGIPLGKSNVTVFSDGEFQ
                     PSFDESVRGCTVFIIQSTFPPVDNLFELLLMVDAAHRASAYKVVAVMPYFGWARQDRK
                     DKPRVAIGAKMVANMLRAAGVDRIMTMDLHADQIQGFFDVPVDHLYASSVFVPYLKSL
                     QIDNIAIASPDMGGAKRANAYARFMNASLVLCYKNREKANQVDEMTIIGDVRGKNVVI
                     LDDMVDTAGTLAKASGLMKDQGALSVRAFATHPVMSGKSYELIRDSQIDQLVVTDTIP
                     LRSDRDTSKIKVLSVAELFADVIEKVYNYKSISEKFII"
     gene            26467..27051
                     /locus_tag="PK839_06235"
     CDS             26467..27051
                     /locus_tag="PK839_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HOG72372.1"
                     /translation="MKTIQLSATLRSETGKKAAKATRKSELIPAIIYGGQKNIMISIG
                     EKDMKKVVYTPDVFVVKLNVDGENFDTIVKEIQFHPVSDAILHVDFYQVSNDKNITIA
                     LPVVLIGQAEGTKQGGKLIQVVRKLKVNGLVKNLPENISIDVTQLELGKSIKVGDLSF
                     KGYTLVDPMSTVIASVKSTRAARETQPTEAAPQQ"
     gene            27063..27626
                     /gene="pth"
                     /locus_tag="PK839_06240"
     CDS             27063..27626
                     /gene="pth"
                     /locus_tag="PK839_06240"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015546856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOG72373.1"
                     /translation="MKYLVAGFGNIGPEYSNTRHNVGFMVLDALARASNITFGSARYG
                     DVAELRHKGKTFILLKPSTYMNLSGKAVSYWMQKEKIALENLLVVVDDLALPLGVIRI
                     RSRGSDGGHNGLKSTNETLGTQDYARLRFGIGNGYPRGHQVDYVLGEWSDEELKILPE
                     RLAKAGEAILCFGTIGIERTMNLYNNQ"
     gene            27639..28058
                     /locus_tag="PK839_06245"
     CDS             27639..28058
                     /locus_tag="PK839_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009437504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HOG72374.1"
                     /translation="MGAESERVDKWLWMVRVFKTRSLAIDCCRNERVTVNGVPVKASH
                     TVQLNDLVAVRKPPVTLTFRVKGFPGSRIGAKLVDEYAENLTPPEEYQKLDPSFRAFS
                     IYRNPGTGRPTKKERRSLDDFFDSEADDSPWDADDSN"
     gene            complement(28096..29346)
                     /locus_tag="PK839_06250"
     CDS             complement(28096..29346)
                     /locus_tag="PK839_06250"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG72375.1"
                     /translation="MKKKTTLYIVIGIVVVAVAAHLLLRPRIAQNSYEKGIVLLDSLQ
                     YGQAYEQLNRAVRFSPKNPDYLFGRGMALAGLNRDSAAIADYTKAIAIDSMNFDYFLN
                     RGVSLRKIGNYTASLRDLNRCISLNDSNSRAFYQRGLLNAAMMEYDQAVKDYNRSIEL
                     DGSNLEAFYSRGESLANISDFKGAIADYDRMIEGKNATPESYKKRGTFKLKIQDFNGA
                     ISDLLVALNDLPNDKELLFSLGEAYSNLKDFDQAIGYLDRAASIDPNDPLIYGTRGGA
                     LLNKGDFKAAIQSFNKAINLKPDYLKAVYGRGIAKAFLKDYRGSIEDFNRAISIDKNF
                     TQAYYNRAISKAIMGNHKDALPDFDIAIKYDPKDAEAFFNRGISRMNVKDTKGGCEDF
                     RKALELGYQPAEQMVKIYCSGSGN"
     gene            complement(29617..>30039)
                     /locus_tag="PK839_06255"
     CDS             complement(29617..>30039)
                     /locus_tag="PK839_06255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72376.1"
                     /translation="LNEYGELVKTEWQKTGIIRPNIVIDAFVVMPNHLHGILIITDNY
                     DCGGTLQRASTTKTTEQFGKPTKNSIPTIVRLFKSTTTKQINQLRVETRCNVSPHVST
                     IPMQPLWQRNYYEHIIRDDTELTRIRQYIINNPKRWGG"
BASE COUNT         7742 a         6738 c         7180 g         8379 t
ORIGIN      
        1 ttgctgatac ggaattttta atacctttaa ctaatcttta acggcggata acgccgctgg
       61 atgttaactc cccagcagcc cataccccaa ttgcattgtg tatctatttt tgataaaatc
      121 agaatttaac ccccgctact gtgcaagccc ttccgtcagc cggctgggaa cttaacgaag
      181 ttattttttt tataatccgg ctggattatg cgttttaaac ctattgttga taaaccggaa
      241 tgtgtttgcc acttgaccga agttgtgggg agtgatgggg gaaaaattca ccccataccc
      301 tcccgtatgc acagcttgga accattgtgc gactcattca tactatagcc actttgaaat
      361 agtagcccga ggtacgggta ataaaattta gctgcagcag ccggttccac agccacaggc
      421 ggccggtttt tcggcataca ccgtaatgct gaaaattcct gtggttcccg atttaaagtt
      481 ttgaagtgcg gcctcgtcaa ggtagctttt caagatgtcg tcgggaatgg taatgggctt
      541 ttctttctgg atggcaatgt ttgtgaatcc gtttttctgg ataagttcta tgtagtggtc
      601 tttttgaatt gctcctgcaa cacaaccggc atacatctcg gcggcattac aaagctcgtc
      661 agggaggttg cccaccagca caacatctga aatgctaaaa tgaccgcctg gcttaagcac
      721 cctaaaaatc tcattgaaca ccctatcctt gttggggaca aggttaagca cgcagttgct
      781 gattaccaca tcggccatgt ttgccgaaac cggcatgcgt tcgatatcgc ctagcctaaa
      841 ctcaacattt gcgaaaccaa gcttgctggc atttgcttta gccttttcta tcatggcggg
      901 ggtaaagtca atgccgataa cccgtccatc cgctccggtt tcgtttcggg ctacaaaaca
      961 gtcgtttcct gccccgctac caaggtcaat aacggtgtcg ccctttttaa ttcctgcgaa
     1021 cttcgttggt aacccgcatc ccaggcctag gtcggcattg gggttgtacc cctccacggt
     1081 actgtagtct tcggtcatga tgttgtaaac ctcagttgag cagcatccgg aaccgcagca
     1141 cgacgattgg ttggtttcct tgtcctgtaa ggcaatttcg ctgtactttt gcctaacaaa
     1201 ttctttaatt ttttcagagt cttccatttt tttctttttt acttacaaca attgtttgac
     1261 ttgaagttaa gcttggctga caggtcgtca aagtattttt gcagcctatt aatagcatct
     1321 tggtttatgc agtagcaaat ggagttcccc tcaatttctc ctttaatcaa ccctgcttct
     1381 ttaagtattt taaggtgttg ggaaacagtg ggttgtgcta atggcagctc attaacaata
     1441 tcgccgcaaa tacaagcgtt cacctttacc agatgctcaa ctatggcaat tctggcgggg
     1501 tgtcccatgg ccttagccaa ctccgaaatg gccttttgct gttcggcgta atgctctgtt
     1561 tttgtaacgc ccatagatat atttaaatat tgcaatatta cgataaaaaa ttttgtccac
     1621 aaggatactt tcaatttttc tctcaattat tttttaacct gaataacccg aactatgttg
     1681 tttaaagctg ccaggaccga caggtagcaa cggattgcta caatttttct gaaagaaaag
     1741 taataaaaaa accccgaact tgtttcgggg tttaatttta attagtttat tcctggtgct
     1801 gagcaacagc catgcggtta tattttttcc agctcggctg ttatcagcgg tctaatttcg
     1861 ttggaagatg gccttggggc atttggttta ataatgttgc ccttggcatc aatcaggaaa
     1921 aaggtaggaa tgcccttaat ctggtagctg gtggcgggta ttgatttcca tgcatcctct
     1981 ccgtaaagct gaacaccctt catctgtttt tccttaacca ttttctccca ggcggccttg
     2041 ttgtcgtcga gcgaaatgct tacaaaaaca atatttttac ccttaaagtc cttttgcagc
     2101 tcctcgaggt aggggagctc atgtcggcag gggccacacc aggttgccca aaaatcaatg
     2161 taaaccagtt tccctctgaa atcctccagg gagtattcct taccgttaat gtccttacac
     2221 acaaaaacag gtgcaggttt cccgggtgcc agctgcatct tttggttgaa tatgtttcca
     2281 acgctctgct tgtacgcggg tgtttggcag ctatccatgt agctctttac aactccttcc
     2341 agctcgtaga actccccgta ctcgatatcg ttaatcagct tgttcatctt taggtagtcg
     2401 cgtacctttg gattagccaa catgctgtcg ataagcttaa attcaaaagg cagcctttca
     2461 cctgaagatt tagccaatag ctccttgtag ccgggttttt tcacaagttg attctgggta
     2521 atgattgata ccaaactggt atactcatca aacatcaggt ggtaggcgtt atttgggtca
     2581 acctcgctga ggaaggagta atcaacgctg tcggggttaa attccttacc acccaggtat
     2641 gcagaatatt cggggtagtt ggccttgacg tttagaatta cataccttgt gcgggtcttc
     2701 tcgagttctg caaggtcttt aggttcaata ctagcagaat ctatcatgga gtacagcacc
     2761 tgctggattg agtcgagctt acggttgaag ttgtcgtagc taagggagta aagggtgttc
     2821 acgcttccca ttaaattatg caaggcagct cttcggctga aaatactttg gtggatgttc
     2881 ttcaggtcag ccgagaaaac cggaccatca acaggtgagg taacatcaaa ttccatgtag
     2941 ctgctatcgc cggggttgag gtaaaccatg gtttgtgtac gggctatgtt aagcttgtag
     3001 agctgaggtt tatcgagctt agccctatag acaaagcctc cattttcatc aagtgccacc
     3061 gtatcggtga tgccggcagt agagatgtac acattactat cgggtgagtt ggtaaacttc
     3121 cccgcaatta cagcctcctt gggccccttg gttccacagg cagccaatac cggaagcatt
     3181 aaaagcaata ggcgtttgtt cattttcatt gagtttgttt gagtttgttt gtgagtatga
     3241 atcattttta ttgagggtaa agttgtaaaa tttttatgtg ccaaccgtac agccggagaa
     3301 cttaaatttt aggtttgaat ttgctggtaa ggttaccttg ttgttcatct accaataggt
     3361 gcatatcatt taccaaagat acttcatttt agagtattat aatgtttgag tataaaatat
     3421 ctattggttt aatttatagc cagccaaagg ttttaatgcc agcaaatata aaaataggtt
     3481 aatgcaagtt gttgtaaggg aaaaacgttg gtttttgcgg actatatttc actaccaggt
     3541 acatataagt taccggcttt aaagtgagca aacccaatgt tattctttat tttgccatgg
     3601 aagggtgaaa aaaattacaa gcattcaacc ttttcttcag tttattatgg agattcctat
     3661 ggaaagcaca agtggttaga ataagcctac cagtagaatc ggcatttgta ggttacccta
     3721 ttgttcagca ggtcagctac ctcgacccta agggtgtgcc aggtgttttg cctttccctt
     3781 ttaggtgtga gggtatgggt tagcaggctg gtcttcgggt cccattcaaa cagaacccac
     3841 ccatcatcta tgaatcctga ataggtgcca attccgtagt tatcggtaag tttaattttt
     3901 atgctgcttt gacccgcaat atccgcattt tgtgaaatat ttagaggaat tatgcgtgga
     3961 ggaacggtgt ccaaagccag ggtaaacttc ccaaacttgt cggggctggt aaccatgtcg
     4021 ggtccatcct gccggctgga gcagtactcg atacctttac cattaatgta tccaataaaa
     4081 catttatcac gcaagttggc cggaacgcct gcaattttta cccttagggt aaatttcccg
     4141 ttaaggggag tcctcgcatg gtgaatgcta attacgggaa atgccaggct gtcggtatga
     4201 agagctgaat gcctgaggag aatgtcatgg taaagggttc ggcggggaat gtagattgag
     4261 taatccttgc tatcgaagct atagctgttc caccagctca catagctgtg tcgcaaaagg
     4321 ccgggggttg attccgggtt tttgaacgga tgaactccct ttactaccat ctcgagtttg
     4381 gaagtatttt tataaaaatc ttcaagttct atggttatct tataaatgct gtcggattca
     4441 accgttaaaa ttcccaatga tttgtctgcc tttaatccgc taaacaaatt accaggatca
     4501 gcccacaggc gtacaatttc ctgttgtcga agttgccgta tgctgtagtc aataatgcta
     4561 tttaggcatc gggtttctgc atacgaaaac gagtcaacag caaaggaaaa ggtctgtttg
     4621 ttatttacca gcatgctcaa cttgtggaag ccgcaggttg tactactccc gttgataatg
     4681 tcgaatgcct caactccaaa acccatcctg ctttttccgg caattctgat ggtgtcggga
     4741 gcaaagtaca ggtttcctgt tttcttaatg ctgatggggt ttggcagtat gctatcgttt
     4801 aggtagtcca tactgtcaat ctcgtaaaac catgcgcttt tgattgttgg ggccgagtta
     4861 tcggccagaa agttaccatc aaggtacttc aatggtgaaa cgggccattg ggttgcagag
     4921 ctgcgaacct caaagtgaag atgaggccca cccgagctgc ccgagtttcc ggaaaagcca
     4981 ataacctctc cctttgacac gttgaactca cctggtttgg gaaatagctc tacttcaaag
     5041 gaatggctgg cgtactgctg cttctctaca tattctgcaa ctgcatttgt aaaacggtcg
     5101 agatgagcat aaactgtggt aagtccgttt ggatgggtta tgtagataac ttttccaaac
     5161 cctactgggc taaccttaat tcgtgataca taccccttct cggatgcata aacccgaaaa
     5221 ccaattttac catttgtccg caaatctatg ccggagtgga agtgtgctgt tcgtaattca
     5281 ccaaaagagc ctgatagtgc aggtttcagg tctaagggtg atttcaggat accattttgt
     5341 gcagccgcct gatggtagtt tagggcagct acaagtaaaa ttaacttatt gaaacgattt
     5401 aacgtacgca tggcttattt ttaaaacact caaagataaa ttatgtacgg atatttagtc
     5461 tggcggacat cgtaaacagc taatattttt gaaaagcagc tggtattaac taggttaaag
     5521 tgaatgggta aaaagtaaag aaaacctgtt cagctttgac aggttcaaga cattctggat
     5581 cactgttatc aattcaattc ttagccggtg ggtgcatgcc tcggtttttc tccccacctt
     5641 gcgtaaattg aaaattgtcg tgtatctttg gcacaaggtt tattcaataa tttatgattg
     5701 aacatcaggc tatgcagcag catgaagtta ccatggacac cgtaagggag ttggggttac
     5761 tccccgagga gtttgagatg ataaaaaagt ttctgggaag ggtgcccaac tataccgagc
     5821 tgagcattta ctcggtgatg tggtcagagc atgcttcgta taaaaattcc ataaagtgga
     5881 taaaaaccct gccccggaag ggttcggcgt tgctggcaga ggctggagcc gagaatgcag
     5941 gcctggttga tataggaggt gggtatgctt gcgctttcaa aatagaatcg cacaaccatc
     6001 cttgtgctgt tgagccctac cagggagctg ctactggtgt aggaggcatc aaccgcgaca
     6061 tctttaccat gggcgctcgt ccggttgccc agcttaactc cctcaggttt ggaaatccta
     6121 agctcgaaag aactcgctgg ctaatgaggg gagtggtgaa gggaattggc gactacggaa
     6181 acgcctttgg cattccggtt ttggccggag aggttttctt tgacgattca tttaacacca
     6241 accctctggt aaatgccatg tcggttggaa ttgtgtcgaa ggattccgta gtatcggctg
     6301 tggcaaaggg tgttggcaat cctgtatata ttgtgggctc tgctaccggc aaggatggta
     6361 ttcatggggc aacctttgct tcggccaaca tcactgagaa ttctgccgac gacattcctt
     6421 ccatacaggt tggcgatccc tttcaggaga agattttgct tgaagccacc caggaggtta
     6481 ttaaaacagg tgcactagtg ggcatgcagg atatgggcgc tgcgggtatt atctgctcca
     6541 cctcagagat gtccgaaaag ggcaagcatg gcatgaggat agatctggac aaggttcctc
     6601 tgcgtcaaac caacatggaa gcatgggaaa ttctgctttc cgaatcgcag gagcgaatgt
     6661 tgatggtggt taagaagggt cgcgaaaggg atgtggagga aattctcaac aagtgggata
     6721 taggatacgc tgttatcggg aatgttactt cgggcgattc gctcgagttc tactaccaca
     6781 acgagctggc tgctaaggtt ccggcctcat cgctggtgct tggcggtggt gcgccggttt
     6841 acgacaggga gtacagcgag cctgaatact acaaggagtt taaaaaattc aacatgaatc
     6901 agattcctga gcctgcagat ctagttgagg tggcaaggtt tctgacagcc catcccaaca
     6961 tagcctcgaa acgatgggtt taccagcagt acgacaccat gattggaacg ggcaacatga
     7021 ctaccaactt cccctccgat gcaggagtct tcaaccttaa agagctaaac aaggcgttgg
     7081 ccatgaccgt tgactgcaac tcgcgttacg ttaaggccga ccccgaggtt ggcaccatga
     7141 ttgcagttgc cgaagccgca cgaaacatcg tttgttccgg tgcaactcct ttggcaataa
     7201 ccgactgcat gaactttgga aacccctaca accctgaagt ttactggcag tttgttaagg
     7261 ccattaaggg aatggccaag gcctgcaaaa agtttgacac tccagtaacc gggggtaacg
     7321 taagctttta caaccaatcg tcaatcaacg ggagggagga agctgtttac cctactccga
     7381 caattggtat gattggcatg ctggaaaaca aaaaccacca tacaacgctg gcttttgagg
     7441 aaaaggggca catgattttc cttattggca aatcacgcaa cgacatagcc tcatcggaat
     7501 acctctacag ctaccacggc attaagcaat cgcctgcacc cttctttgac ctggatgagg
     7561 aatttgccat tcagcaggca ctgctcgacc tgattgctag gaatttggtg caatccgcac
     7621 acgatgtttc tgatggtggc ctctttattg cccttcttga gagtgccatg ccccgtaacc
     7681 tgggtttcga tatcactact gatgccgaga ttcgaaccga tgctttcctg tttggtgagt
     7741 cgcagggtcg aattgtagtg tccgtttctc catcgcgtga aacccatttc attgactaca
     7801 tggttgagca tggcatcccg tttaccgctc tggggcatgt aaccaaatcc gagatcagaa
     7861 ttgacgatgt ttcgtttgga tttgttgccg atttgaagca gctttacgac actgctctgg
     7921 agaagatcat ggcctagtat tcctaacgct ttatgccaac acctgacaaa agcagccaga
     7981 acgttgaggc catgtttaac agcattgcct ctaactacga tttcttgaac catttcttaa
     8041 gccttggaat tgatagaaag tggcgtaaac ggttaatccg gttggtagct aagggtaacc
     8101 cacagaaggt tcttgatgtg gccacgggaa cagccgattt ggccatgctg ctggcgcggg
     8161 agtatggagg tgtttccatt acaggggtgg atatttcgga gaacatgctg gccattggca
     8221 agagaaaggt cgaatccgaa aatctttcag gtcagatttc gctccacagg gctagtgcat
     8281 tgaacctacc ctttgaaaac gactcttttg atgctgccat ggtggctttt ggggtaagaa
     8341 attttgagga tttgtcaatg gggttatcgg aggtatatag aatccttata aatggcgggc
     8401 agtttgccat acttgagttt accattcccg ataagttccc tgttgcccag ctttacaggt
     8461 tctactttag gcacgtgctg cctttgttcg gggggattat ttcgggcagc cggacagcat
     8521 ataaatacct gcccgaatcg gtacttgcat ttcctgagaa ggagaatttc ttagaaatca
     8581 tgaggcaagt tgggtttaca ggttgcacat gcaagagttt aactttcggg attgcttcaa
     8641 tatatacagg ctttaaacct taaattcatt ttgtgcatga agccatttca ggggagaggc
     8701 cctgattgct tattcaacaa ccgaccatta gaaaccctac cacgaatgtt tgacgtctgt
     8761 ttaagctatt gtttgcaata ttttttatac cctgcacgtt tgataacagg ctttacctat
     8821 cgtacataaa tgttcaggtg cagtatccgt ttgagagtca ttcatccaag gtaatttttt
     8881 tacgtagatg ttttacccta agagtttata actattttga agaaaatctt tgttgtaata
     8941 gcggttatct ggtgctcggt gactgcagct caggcgcagt accgtaagcc taacattctc
     9001 aacgatcccg attatgacgt tggcaaaccc ctaaggtttg gtttctccat cggcatcaac
     9061 attatggatt ttgatattaa gaacagctcc gttcccgagc ttaatgccga cagctcccag
     9121 ttccagtact tcacagaggt tacgcatata aacccgggtt taaatgtcaa cgctatttcc
     9181 gatttaaggc tagccgaaaa ttttcaccta cgctttttac cgggttactc ctttgggcag
     9241 cgaaatctcg atttttttaa ggtcgaaaat ggacgggtta cccatgaaac tacaatggat
     9301 attgagtcga attttattga tttaccttta ggcattaagt accttgccga gcgaaccagc
     9361 aacattaggc cgtatctata cgtgggtggc gattaccgta ttgatttagc tgcctataaa
     9421 cggcttaagg tcgaaaaggg aatactggtt aggctgcaaa agcaggattt ctactacgag
     9481 ctcgggtttg gtgttgactt cttttttgaa tattttaagt tttcaacaga ggtgaaatgg
     9541 tcggcaggca ttggcaacgt tatttcaagc gactatgccg agggggccga gaactaccgc
     9601 tatgctgttg atgctatgaa ctccaggctg ataatgattt ccttccattt tgagtagaaa
     9661 agtggaagtg tagcataaaa caggctgttc atccatcttc ttgtagttgg ggtaatacgc
     9721 ggaattttga tataccctca atcagctata aattgttcac ctcgccgggt tttagggatt
     9781 attgagaagg ggcttaacac tattgtttgg agaaattcct ttttttcaca ctgatgctga
     9841 taatttttca ccatggctaa ggatataatt cttgaactca cccctgaaaa tgcggccaac
     9901 agccagctat tgctgaaagc agttgccagt aagctgggaa tgagtgttaa agatgtcggc
     9961 cattacacta ttacccgacg gtcaatagac tcacgacacg gaaaggtaag ggtaaacctt
    10021 ggcatcaggg tttttgatag ggatgaacct attgctgacg gtaaacccca attcacctat
    10081 ggcgatgttt ccaaatcgcc tcctgttgca gttattggcg caggtcctgc tgggttgttt
    10141 gccgcattaa ggcttattga acacggctac aagccaatag tggtagagcg gggcaaagaa
    10201 gtaagcgccc gcaaaaggga tgttgccctg ctaaaccggg agcataagct caaccccgat
    10261 tccaactatg cttttggcga aggaggtgca ggaacctttt ccgatgggaa actctacacc
    10321 cgttcgcgaa aaagaggtga ttacaatcgt gtgttgtaca tcctttgtaa ccacggggct
    10381 agcgaggata tacttttcga ttcgcacccc cacattggaa caaatcgctt gcccggcatt
    10441 attgtgaaaa tcagggagac aatcattagc tacgggggcg aaattctata taactcccgg
    10501 gttgaaagta ttaaaaccga cgggagcagg gttacgggtg ttaaactttc cgatggccga
    10561 cttgtggagg ccaaggcctg catactggca accgggcatt ccgctcgcga cgtgtattcc
    10621 atgctgttga atcagcaaat tcagatagaa gcgaaaccct ttgccatggg tgttcgggtg
    10681 gagcatcctc aggctattat tgactccata cagtaccatg gtactcctcg gggcgactac
    10741 ttgccagcag catcctactc gcttgtgcag caggtcgaag gaaggggtgt ttactcgttc
    10801 tgcatgtgcc cgggagggtt cattgttcct gctgccactg aacccaacga ggtggtggtg
    10861 aatggcatgt ccccctcact tcgaaactcc cgctgggcaa actcgggtat agtggttgag
    10921 gttaggctcg acgatttgaa gtcctactcc aaatatgggg agcttgccgg tgtggagtac
    10981 cagcactacc ttgaagagtt ggccagcctt catggcggtt ttggtgtaaa agctcctgca
    11041 cagcggttgg ttgactttgt ggatggaaac tactcggcga gcttaccaag ctgttcctat
    11101 catcccggca ttacttcctc cgaacttcac gcctggcttc cagatgatat tgggtttagg
    11161 cttaggaaag ggttcagggc atttggtgca agaatgaaag ggttcctcac caacgaggcg
    11221 gttgtgctgg gagtggagtc gagaacttca tcacctgtga gaattccccg taaccccgaa
    11281 actcttcaac atgtaggggt ggaagggcta tttcctgccg gagagggcgc cggctatgcc
    11341 ggtggtatag tttcttctgc ttttgacggc gaacgatgtg ccgacgctgt ggctttctac
    11401 ctgggcggtt aaaagtgatt caccaaagcc ttgagctgga aatggattgt gatgcacctt
    11461 cctgcttaaa tcggaataaa gcagctaccc ataatgtcct tagcgccatg gtaaatagtt
    11521 agctaacgac acgctaagcc ggattggcgt gtgaacaaaa tcaacaagtt gaaagttttt
    11581 ttgatacatt tgttggcaat ataacaagta gaatgctgta ggcaaacgtc cggactgcta
    11641 actattgatt ttgacatggg acgaaaaatt gtagtggggg taacaggcgc cagcggtgcc
    11701 atatatgccc gttcgctgct agagattttg ggtgagcttt ccgatcagct ggaggaggta
    11761 gcgcttattt tttccgataa cgggcgtgca gtttgggagt ttgagcttgg ccatattcca
    11821 cctttttctg ctcctttccg cgtttacgac aacaacaacc tgtttgctcc tgtggcctcc
    11881 ggatcggctg ggttcgacac gatgtttatt gcgccctgca ccatgggaac cttgggaaga
    11941 atagctgcag gaaccagctc cgacctgatt tgtcgatcag ccgatgttat gctcaaggag
    12001 cgacgtcggc tgatcgtagt gccgagagag ctacccttga gccttataca tattgagaac
    12061 atgcgaaccg ttaccctcgc aggcggtatt attgttccgg ccagcccatc gttttatagc
    12121 cgcccttcat ccatggaaga gctggttagg actgtcgttt tcaggatgat tgatttggca
    12181 gggcttgagc tgcctcatta ccgatgggga gaagctaaaa gcgattcgta gcctgataaa
    12241 ttcgaatttt tttaccatgc agggggagga gaaagttgtg agtaggtaca ttactaattt
    12301 aattggcatg ggggaggggt tgaccctcga tttcaagcat tccatcaccg attcaaaaaa
    12361 gattgcccgt tcattggctg catttgcaaa cacccagggc gggacgctgc tggttggggt
    12421 aaaggataac ggcagcatta cgggtgtcga taccgatgag gagtactaca tggtcgaagc
    12481 tgcctcaaca ctttactgca ggcccgaagt accttttgag gtggttaaat ggaacatgaa
    12541 cgccaaaacc gttctggaag tgaaggtaaa accttcaaaa ttacgccccc ataaagcccc
    12601 cgataagaat gggcattaca aggcctacat ccggatagcc gacgaaaaca tggtggttac
    12661 atcaacccag cttaaaattt ggaaggcgga acgttcgcgc aaatccatcg gtcttaacct
    12721 atccaatgcg caaacagccc tgtttgaaca tttcaaacag aatccacgca ttaccattga
    12781 cgagttctgc aaaattgggt ttctcccggc tactgaggca gagcgtattc tggttaacct
    12841 caccgtttta ggaacaataa ggtattcagg taatcctgct gacgaatatt tctacccttc
    12901 cgtctaaact ttttatgctt attatggttt tatggtctaa aggcaatagc tgcttacggt
    12961 ttatcttttg tagagttcat gttgaccttt cttcattttt aatgaagtaa ccgggcagga
    13021 cacagtttgc ataagcacgt atgaacaacc aggcgttact tgcaagtccg gtgaagctaa
    13081 aatttaggcg ttacattcgc tcagcccaaa tgcctaaaag gcatgagcta aagtagctgt
    13141 aatcggaaac ctgaaaattt tgaacagatg aaaccattat gatcagcata attttttatt
    13201 agcgtctatt tacagggcaa tggtattttt tagcgtgaaa gaccccaatt ttttttttac
    13261 tttgtaaaaa ttttgaagga tgacagaagt tagaaaacct gcctggttaa aggtgaagtt
    13321 gcccggaggc gagggttaca gacgggttga cagcatagtg aagaataata gcttacacac
    13381 gatttgttcc agcggaatgt gtccaaacat cggcgaatgc tggggaaacg gtgtggcaac
    13441 atttatgata cttggtgata tttgcacccg tgcatgcaaa ttctgcgcta ccaccaccgg
    13501 aagacccctt ccacctaatc caaatgaacc agagaaaatt gcccgctcca ttcagctaat
    13561 ggcgctgaac tactgcgtta tcacctcggt tgaccgagac gatttgcccg atcagggagc
    13621 cgctcactgg cgtaacacgg tgaatgctat tcgtagggtg aacccaaata ccatgataga
    13681 agttcttatc cccgatttcg acgggaggag cgatcttatt gatattttcc tggaatcaaa
    13741 ccccaacgtt gttgggcata atcttgaaac agttgagcgg cttacccctg aggtaagaac
    13801 aaaagcccga tacagaacca gcctggaaac ccttagctac attgccagca ggggttttac
    13861 aaccaaatcg ggaataatgc tgggtatagg cgaaacgttc gatgaggtag ttcaaaccct
    13921 cgacgatctt tccgctgtgg gctgcagaat gataaccatt ggccagtacc ttcaacctcg
    13981 gccgaacaac ctgcccgttc agcgttatgt tactcccgaa gagtttgagg agtacgccag
    14041 aatagcccgc caaaaagggt ttgtatttgc cgaagccgga cctatggttc gttcctccta
    14101 ccatgccgat aaggcacaga agctgtttgg aactcgacct gatagtgttt aaatgcttta
    14161 cccaccatga gaaaaagaaa aaatacatat aatgcaaaac gtaattttta cggatcttgg
    14221 aacaatagat tacaaggaag catgggatct acaggagaaa ctattcgatg aggtaagcct
    14281 aaacaaggcg gagggcgatg gcaacctgaa cttcctgctg ttctgcgaac atccgcatgt
    14341 ttacaccctt ggaaaaagtg gccaaaggag caacctcctg gtctccgacg agatgctaaa
    14401 gcgtatcaac gcctcctttt accatatcaa caggggcgga gacatcacct accatggccc
    14461 cggccagctg gttgcctacc caatttttaa ccttgaggct tttggcctct cgttgaaatc
    14521 atacatcagc cttattgagc aggtggttat agactgcctc ggcgattttg gagtaccggc
    14581 aaaccgtctt gatggagcta ccggtgtttg gcttgatgcc ggcattaagg gcaaggagag
    14641 gaagatttgc gccatcggtg tccgggccag ccgttttgtt accatgcatg ggcttgcttt
    14701 caacatcaat acaaacctcg actattttgg atacatcaac ccatgcggct ttaccgataa
    14761 aggcgttaca tcgttacagc gggaacttgg agaagaggtg aatcctgaaa tggtgaaaca
    14821 gcgaatttta gaccattttg ccagaaagtt taattttact gtaaacacac ggctttttcc
    14881 gaacctgaag ctagctacga actatgaggg agggaagcac aatggataaa cgttgggttt
    14941 tcaaggatgt tgacggcgat gaagccgtta acaagcttgc tgctgagctg aatattcctc
    15001 acacactcgc ctcgctactt gttcagcgtg gaataaagac atttgatgag gccaaacggt
    15061 ttttcagacc atctcttgac gatctttacg atccttttct tatgaaggat atgaaggagg
    15121 ctgttgaccg tatagttaca gcaattcatc acggagagtc aatccttttt tatggcgact
    15181 acgatgtaga tggcacaacc gctgttgccc tgttgtattc tttttttcgc cagcgttact
    15241 ctaaaattgg ctactacatt cccgacaggt atggcgaggg ttatggaata tcatacaagg
    15301 gcattgatta cgcttttgag aatggttaca gcctaattat agcacttgac tgcggaatta
    15361 aggccaacga aaaggttcta tacgccagcc agaggaaaat agattttatc atatgcgacc
    15421 atcatttccc tggcgatcag gtgcctggtg cggttgcagt tcttgacccg caacggcccg
    15481 attgccacta cccctataag gaactttcgg gttgtggggt aggttttaag ctgcttcagg
    15541 gatactgcct taaaaccggt acccccatgc aggaactttt ccagcatata gacctggtgg
    15601 ctgttagcat tgcctccgac attgttccta tcaccgacga aaatagggtt ttagcatact
    15661 atggccttga aaaaatcaac tccaacccat ccaagggcct taaaacaatc attcagattg
    15721 ccggaattga taaacagaaa atagccattg acgatattgt ttttcgaata ggccctcgta
    15781 ttaacgctgc cggccgcatg gaatcgggca aatcggctgt tgatttgctt tgcgcagccg
    15841 atgacggaac cgcatacgaa atgggatcgt tggtaaactc gtgtaataac gaccgaaaga
    15901 atgttgaccg aagcatcacc cacgaagcgc tcaggatgat tgctgccgat gccaggctcc
    15961 agcactgcca ttcaacggtt ctcttcaacc cttcgtggca taagggtgtg gtgggtatag
    16021 ttgcctcgag gttgattgaa acctactaca ggcctacagt tgtgcttacg caatcgggtg
    16081 atttggcaac cgggtcggca cggtcggtac cgggctacga tctctaccag gcggtagagg
    16141 cctgctccga cttgctcgaa aactttggtg gccacatgta cgctgcaggg ttgtcgctta
    16201 agcttgaaaa tgtcgagaac tttaaaaacc gtttcgagga gtacgtaagc caaaccatca
    16261 ccccggagct tatgatgccc caaattgaga ttgacgccag aatcaagctg agcgacatta
    16321 acgataagtt ctaccgcata cttaagcagt ttcagccttt tggccccgga aacatgtcgc
    16381 ctgtttttgc tgctgaaaat gtgagggata acggcgaatg ccggctggtg ggtcccgata
    16441 aggaacacct taaactttcg ctggctgagg agggcaatcc taatatctac cacggcattg
    16501 ccttccagct gggcgagtac taccgcgata tccacaatgg aaaatcgttc aacgtctgct
    16561 tcaacattta cgaaaatgtt tacaggggca ttaccaccct gcagcttagg gttaaggact
    16621 tgaagtttat cggaaatcag gattgacact aacatgctaa aaaacagata tactggaaat
    16681 tttgagcatt atgatttcat gggacttaca cagcataaaa ctacgtagag ctactttttg
    16741 gttaaatttg gctgagcaag caaataattc cctattttta taaaggaaaa ttaaacgagc
    16801 aatcgcttga aagcaaggta cttgataatc ggagcagtgt tgctgcccgt tgcttcatat
    16861 ggtcagaagc agctgcaacc taccattgcc gccatgaaat ggggcgttga ctacaagctg
    16921 catgttaggt tggcaaacga ctcggcctat gttgttgatg ttagaggcct ataccacacc
    16981 gggagggatt tgtttgttga cactaccgac aactctgcca cctactatcc ggtaaccctc
    17041 gacagggagt ttgtggacta cattaagagt aagaaaatcg acaccagcga cagcctctca
    17101 ctggccgata gcaaccgcac agctagctat aaaacgttat ggtcggcgct gcattccaat
    17161 cttggcggtg ggtacattca ctttgttaac tgcttgattt acgccctgga gtcgcaaaac
    17221 ctaaagctta acgaccccat attcaagcgt cctgctacca ggtggaagcc taatcccgta
    17281 acccaatcgt acaagcgtac acataagtgg agctattttt atcctgacaa ccagaagctg
    17341 gcgcaaaagg agtacaggat acgcctgaaa gaaaatgacc tgaaagacct tcagggtgtt
    17401 ccacttcagt ttaccaacct gtttctgcac acttcccaaa agaagtatga agagctggtt
    17461 cggcaacaaa agaaccggga ggttgcacag attaacctca tcaggttgct gcttggtgct
    17521 aagtatttag gcgaggatca gattaggcat ataagcaaca gcgtaatttc tgccgtgctt
    17581 aagtacaacg ttaacaccct tccctccatc atcatttttg acgactacaa tgcagctgta
    17641 gcactaactc tcgatatgaa cggctaccgg attgatagaa tcgtttactc tgatggcgag
    17701 gtgatttcgg gaaaggaact cgagggtcgc actgcaatta ttgatgggct gattaggaat
    17761 atcaacgagg ctaacgaaag ggtttttcag aaaaagttac agctttacta ccaaaaaacc
    17821 agataggatg agcctctcct tggattcgta aaataatcgg ggcttgctga tctccatagc
    17881 agcttaagta ctcacgccta ttagcgaggc tacaacgttc acttcaattt ttgaagattc
    17941 cgccatatag gctgtttcat ggctgggctg ctcactccat ttaacattca gggttggcta
    18001 ctcttttagc ttgcggtact taattcgttg gggaccctca tcgcccaacc gttttctttt
    18061 attttcctcg tagtcggtgt atgaaccctc aaagaagtaa acttgcgaat ctccctcaaa
    18121 ggcaagtatg tgggtggcga tgcggtcgag gaaccaacgg tcatgtgaaa ttaccactgc
    18181 acaaccggca aaatcctcaa gaccctcctc cagcgccctg agggtgttca catcaatgtc
    18241 gttggtgggt tcgtcgagca gcagcacgtt tccttcctcc ttcagggcta gcgccagatg
    18301 aagccggttg cgctcaccac ccgatagcat tccgcacttt ttctcctggt cggcgccgct
    18361 aaagttgaag cgggccacat aggctctggc gtttatcgtc ctgctgccaa gcatcatgct
    18421 atcgagaccg cccgaaataa cttcaaaaac agttttttcc gggtctattg cttgatgctg
    18481 ctggtcaaca taggccagct taaccgaatc gcctattcta aactcgccat agtcgggcgt
    18541 ttccatgccc atgataagcc tgaatagcgt tgtttttcct gctccgttgg ggccaattac
    18601 accaacaatg ccattaggtg gaagtataaa attcaggtgc tcaaatagca gcttttcacc
    18661 gtacgatttt gccacgtctt tagcctcaat aaccaagttc cctaagcgtg gtccgttggg
    18721 gatgaaaatc tccagctttt cctccttctg cttgacatcc tcgttcaaca gcttgtcgta
    18781 ggccgataga cgggctttcg acttagcctg acgggctttt ggcgacatgc gtacccactc
    18841 cagctcgcgc tcaagggttt tccggcgttt gctttcctgt ttttcctcct gtgccaggcg
    18901 ttgagttttc tgctcaagcc acgacgagta gttgcccttc cagggtatgc cctcacccct
    18961 gtcaagttcc agtatccaac cggccaggtt atccagaaag tagcgatcgt gggtaatggc
    19021 tataaccgtt cccttatact gctgcaggtg ggtttcgagc cactgcaccg attcggcatc
    19081 gagatggttg gtaggctcat cgagcaggag aatatcgggt tcctggagca gcagcctgca
    19141 tagggctacc ctacgacgtt caccgcctga gaggatttta accggagtgc tgccctcggg
    19201 gcacctaagt gcatccatgg ctcgctccag cttggaatcg agattccatg cgtcggcatg
    19261 atcaatcaac tcggtgagtt caccctgcct ttggatgagc ctattcattt gctcgtcgtc
    19321 cataggctcg gcaaatcggg cattcaactc ctcatattct tcaagaagtt taactgttcc
    19381 ggcaacacct tcctgaacaa cctcctttac tgtcttgctt tcgtcgagat gtggttcctg
    19441 ttcaaggtag ccaatggaat agcccggcga gaataccacc tcgccatcaa aggatttttc
    19501 aaccccggca attattcgta gcagggttga ctttccggag ccgttaagcc caattattcc
    19561 aattttggcg ccgtagaaga acgacaggta gatgttgttc agcacctttt tttgaggtgg
    19621 aaatgtttta cacaccccaa ccatggagaa aataattttt tcgtcggcca tttacttcgc
    19681 tgttacaggg tataaaaaat accgctggcg aagcatgtta accagcggtt ggttagtgta
    19741 atgctattta gctgccaatc tttttgaagt ccttaaccag gaatccatca ccggctgatt
    19801 tcgagatggt atgcatgtcg tagtagtaca tcatcccatc gccagcgcca ataatcattt
    19861 tccagacgcg ccattgcagc cttgttccct cgggaccaac cttgtgtagg aatactacat
    19921 tcgggttgcg ttccaaaatg gctttctgaa tatcctcggg tttaacaacc ttgatggagt
    19981 acttatagtt gttcctgatg tcagttaggg cacgggtggt tttatcaaga ctgctttcgg
    20041 ttacccataa ctccttgttt ttaatttgct tcatgttctt gttgtagagc ttaaaggcgt
    20101 cctttttaat gagttttggg ttggtgttga tatccctgat gtagttctgc atgaagttaa
    20161 tcaacccttc gagtttgtag gtgtaggtat cttctgaaac ctccttgtag cttaaaggaa
    20221 ctgagcatat atcgggcata tcggttaact ttttgacggc agcccctagc gatacgctta
    20281 agaagttgta aacgggtgcc accttatcgt tgtcgaacct aacctgtaac agcaccagaa
    20341 aagacttgtc tatatcctga cgcacctttt caaactcctt ggatgtgatg aactcgtagg
    20401 gtgtaatttt ccatgagcgc tcaacagctt cacgaatttc gatgttatac tccgatgtag
    20461 ggttttcctc aagcacaatg taagttttcg acgccatgaa tgttttaaca tcctcaggtc
    20521 ttaccagatt tctttgggca tagaggtttg ccactccaaa aagaatcgct aatgttactg
    20581 caaatttttt catgagctta gacattttta accacgtaat ttacaaataa ttccttaaag
    20641 agcaattcgc cggaagtcaa cctgaactaa attttctgaa gctggttaat ctctaccatt
    20701 tggcccggca aaatgagatt atgcatgatt ccaggttctt tacctctgca tttatagatt
    20761 gcaaatttat acttcttttt ttaaaaattc atgatttaat caaaggttcc ttaacaaaat
    20821 tagggctaac ctgaaaacaa aaaaatgttt ccctccaggg taagcttgaa cggagctaag
    20881 ttcaaaaaag ctaaatcggt aaattttatg tagatttgaa cggtggaaat ggtggcagtt
    20941 accagtataa attgctatat atcaacttat ctgcatacat tctcattcaa attcagaaaa
    21001 cctgggagca atggagaaga agaaaattgt ggatgttcac gaggtaaaaa aggtggtcaa
    21061 cctagggcct attgttggtg atggggtagc ccatttgata atgtcggccc tgcggttcaa
    21121 caagctgaac gaaaagtact caaatatatc ccacctgaaa ggattggagt tcctttcggc
    21181 agcaatggat acctttgaga taacctacga ggttaacccc gaggagctca aacgaatacc
    21241 ccgaaccggt gcgttcattt cggtgtcgaa ccatccgttt ggtggggttg acggtatgat
    21301 tcttattaag atgatgctgg agcagcagcg cacggatata aaaattctgt caaattttat
    21361 catccagcgt attccccaga tagcagagtt tgtgttcccg gttgacccat tcgacaacgg
    21421 agttaagcgt ggatcgaatc tgatagggct caaaaaggct ttaactcatg ttagcaatgg
    21481 gggggcatta gggcttttcc ctgccggaga ggtttcaacc tactacgata acatggatga
    21541 ggttggtgta gttgacaggc aatggcaacc atccatgatt aaatttatta aaagagcagg
    21601 cgttccggtt gttcctattt actttcaggg tgaaaatagc cgtatattcc accttttggg
    21661 acttattcac cctatgcttc gcaccgccaa gctaccttcg gagctgctta ataagaagaa
    21721 ccaggttatc agggtgagaa ttgggtaccc catcagcgtg gaggagcagg atgcctactg
    21781 cgatttggac aggtatggcc gattcctcag ggttaaaacc tatgctttgg gttcgtcgct
    21841 tgatgtggat aagtttctta gctacaagct aaggtctgcg ccagtacccg aacctgttat
    21901 accgccggtt ccatttagcg cggtggaaaa cgaggttgaa aagctgctca gcaagtacct
    21961 gcttttccgc agcaaaaact acgcagtggt ttgtgcccca tcggttgaga tgcccaacct
    22021 gatgaccgaa attggccggc taagggagat tacctttcgc gaggtgggag agggaaccaa
    22081 ccggaaaatt gatgtggatg agttcgacct ttactactgg cagctcttta tttgggatga
    22141 ggaggagaaa agaattgtgg gtgcctacag ggcaggaaag ggcaaggaga taatcgaaaa
    22201 ctatggcatt gaaggctttt acatacaaac ccttttcaaa attgataagg agtttgcccc
    22261 tatcctggag cagtgcattg agctggggcg ctccttcatt gtgcccgact accagcgcaa
    22321 accactgccc ctattcctgc tctggaaggg aattctctac ttcctgatta aaaatccgga
    22381 ataccgctac ctgattgggc ctgtgagtat ttcgagccgt ttctccgatt actccaaagg
    22441 ggttatcatc acctttatga aatcgaatta ctttgaccat aacttcgcaa ggttcattcg
    22501 tccgcgcaac agctttcggg tgcctgtgaa ggaggaggag ttcaaaataa ttttcgacac
    22561 ttccgatgac ctcgacaaac ttgataagtt catcaaggag gttgaaccct acgactaccg
    22621 catgcccgtg ctacttaaaa agtacttgaa gctcaacggt agaatcctag gctttaacgt
    22681 tgaccctaag tttaacaatg cccttgacgg tttactaatc ctcgaccttc ttcaggtccc
    22741 tattcagacc attacctccc tgtcgaaaga aattaacgat aagtccattc tcgagaggtt
    22801 caacattagc gattatactt ttgaggatta aaatgcagct tgtgtagcct gctttaccgt
    22861 ttaacctatt ggagctagtt gtttgtttgt agaatcccct tcgtgtacgg gtacagggga
    22921 tttttttcaa ttcttgatgt tttctagcta aaccataacc gatttgttcg gtcaattttt
    22981 ctgttcgata gctggctata aagtttgaaa acagcttttt tgaaagttta cgcagctgtt
    23041 aaatatgggg aattgtagtt tttttgtgca ttttctttta ttatccttta gtcttttttt
    23101 aaattttctg gtaaaatgag attagcattg tactttctgc tggcagcaat atttgcactt
    23161 tcgtgcaaca ggcaagtagt gagtgaaccg gagttggttg agcgtgccaa tgagctgcat
    23221 aaaaagattg taaccattga tacccatacc gatgccccac tcaccttcat gcgtaccggg
    23281 tatgatttca gcggtaaaaa caacaaagcc attggtagct gtgttgacct agccaagatg
    23341 gaagcaggtg gtctggatgc cgcatttttt gctgtattta taagtcagga tgaatgcacc
    23401 cccgacgcat accgaaaagt caacgcacaa gcccttcaaa ttgcgagtac cgttataaac
    23461 accgttgcca agtaccccga cagggctgaa attgctgtaa accccgagga tattttccgg
    23521 ttgaaagctg cggggaaacg agccatttac attggcatgg aaaacggata cccgcttggg
    23581 gaggacttat cgcagcttaa ggttttctat gatttgggcg ctcgctacgt aacactctgc
    23641 catacccgga acaaccagat ttgcgactcc tcaaccgacc ctgaaggttc aaggaataat
    23701 gggcttagcg atttcgggag atccgtagtg gccgaaatga acaggctggg tatgctggtt
    23761 gacgtttcgc atatctccga caagtcgttt tacgacgtgc ttgccgtttc caaggtgcct
    23821 gtttttgcct cgcacagctg tgccagggca atttgcaata accctcgaaa ccttgacgat
    23881 aacatgctga aggccctagc taaaaatggc ggggtagttc aaatgtgtat tctcagcgac
    23941 tacgtcaagc agggtgagaa gaatcctgta cgcgatagcg catttagggc gctgcgtcgc
    24001 aagtatggca acttccgaaa ccttactccc gaacaggagg agcaggctga ggcagactgg
    24061 cacaacctcg agcagcagta ccctgctaag cttgccacgg tatccgacgt ggttgaccat
    24121 atcgaccaca ttgttagggt tgccggaatt gaccacgtgg gcatcggaac cgacttcgac
    24181 ggcggtggag gtgttgatgg atgtcgcgat gcctctgagc tacacgcaat aaccattgaa
    24241 ctcctaaggc gggggtattc cgagcgggat atcaggaaaa tctggggcga gaatttcctg
    24301 cgtgttttcc ggcaggcccg cgattatgcg gcagctcatg ggcagggcaa gtagctattg
    24361 ctgagttgct cacaatagta atacctgcat caccatagct gaattttctc cattcatcct
    24421 gcaatcaaca caccagccag ctcatggctg gtttaacctt tatctaacgg cgtattatgc
    24481 tgatgtggct actacccaat gagctatttg caccggatat aaagctatcc aagcaagttt
    24541 ccccgagcgt tcacctttat tctcagctta ttattcccga tgaaggatat tttgctgtgt
    24601 ttttctattt gtatgtattt tctactcttt tgaccaatgg tttgcttttt aattttatag
    24661 gtttgtatct ttgaggtgga ttctgggaca atacatcaaa ctaaaattta caactatggt
    24721 gattcgatct gctaaatttg tagcgagcta ccccggtgtc gataggtgcc ccgcttcgtc
    24781 aattcccgag tatgcattta ttggccggtc aaacgtagga aaatcgtcgc taatcaacat
    24841 gctgacgggg accaaaacgc tggctaaaat atccgaaacc ccggggaaaa cacagctcat
    24901 caactatttt ctggtaaacg agtcatggta tctggttgat ttgccgggtt atggctatgc
    24961 caaggcttca aaagacaaac gaaaggagtt ttcccaaatc atcacaaatt acatacttaa
    25021 acgttcaagt cttaccctac tttttgtgct gattgattca cgtcttgaac cccagccaat
    25081 agacattgat tttattaact ggctgggtgt aaaccaaatc ccgctagcgc tggtatttac
    25141 taaaaccgat aaaacgccta aagctctcct tgaggccaac atggaagctt ttaaaaataa
    25201 aatgatggag atgtgggagg tgttacctcc aatatttacc tcctcggccg ttacccgaac
    25261 tggaagggag gatgtgctat cctacatcga cagcataaat gcaaatttta agtcaaccca
    25321 gcccaaaaag gttgaacggg tctggtaaaa ttagtacatt tgcgggcatt aacaggaaaa
    25381 aattcctaaa atgcacagca agcacaacat taagttcttt accggtcgca gctcccggta
    25441 tctctctgaa agaattgccg atagttttgg cattcctctt gggaaatcca acgttacggt
    25501 atttagcgat ggcgaatttc agccttcctt tgatgaatcg gtgaggggct gcaccgtttt
    25561 tatcatccaa tcaacctttc cacctgtcga caacctattc gagttgctgc taatggttga
    25621 tgcagcccac agagcttcgg cctacaaggt tgttgcggtt atgccctatt ttggttgggc
    25681 caggcaggac cgaaaggata aaccccgcgt ggccattggc gcaaaaatgg ttgccaacat
    25741 gcttagggca gctggcgttg accgtattat gaccatggac cttcatgccg atcagattca
    25801 aggtttcttc gatgtaccgg ttgaccacct ttacgcctct tccgtttttg ttccctacct
    25861 taaaagcttg caaattgata atattgccat tgcctcaccc gatatgggtg gcgcaaaacg
    25921 tgccaatgcc tatgcccgat ttatgaatgc ctcgctggtt ctatgctata agaaccgcga
    25981 aaaggctaat caggttgacg agatgaccat tattggcgac gtacggggta aaaacgttgt
    26041 aatactcgac gacatggttg acaccgccgg aacgctggcc aaggcttccg gtttaatgaa
    26101 ggatcagggt gcgctatctg ttagggcatt tgccacacac cccgttatgt cgggcaagtc
    26161 atacgagctc attcgcgatt cccagattga ccagctggtg gttaccgata ccataccgct
    26221 gcgtagcgac agggacacct ccaagattaa ggttctctcc gttgccgaac tttttgccga
    26281 tgttatcgag aaggtttaca actacaagtc tatcagcgaa aaatttatta tttagcgttg
    26341 tgtttttaaa gccttttcat atctttgcag ccctaaatat ccctcaaaat taacggtgtg
    26401 atggggggct gaaaaatttt cgagggcagt cctgagtagc acctattggt taacaaaatt
    26461 tctacgatga aaacaattca gcttagtgcc acgctgcgta gcgaaaccgg gaaaaaagca
    26521 gccaaggcaa cacgtaaatc cgaactcatc ccggcaatta tttatggtgg ccaaaagaac
    26581 attatgattt cgattggcga aaaggatatg aaaaaggttg tttacacccc cgatgttttt
    26641 gtggttaagc taaacgttga tggtgagaat ttcgacacta tcgttaagga aattcaattt
    26701 cacccagtat ccgatgccat tctgcatgtt gacttttacc aggtaagcaa cgataaaaat
    26761 attaccattg ccctcccggt tgtgctaatc gggcaggcag agggcaccaa gcagggtggt
    26821 aagttgattc aggtagttag aaagctaaag gtaaatggtc tagttaagaa tctgcccgaa
    26881 aatatcagca ttgatgttac tcaactagaa cttggcaaaa gtataaaggt tggtgatttg
    26941 tcgttcaaag gctataccct ggttgatccc atgagcactg tgattgccag cgttaagtca
    27001 acccgtgctg cacgcgaaac tcaaccgact gaggctgctc ctcagcagta aattgacatg
    27061 gattgaagta cctggttgca gggtttggaa acataggacc ggaatattcc aacacccgtc
    27121 ataacgtagg atttatggtg ttggacgccc tggccagggc gtccaacatt acttttggaa
    27181 gtgcccgtta cggtgatgtt gccgagctac ggcacaaagg caaaactttt atcctgctaa
    27241 aaccgtccac ctacatgaat cttagcggta aggcggtgag ctactggatg cagaaggaga
    27301 aaattgccct tgaaaacctc ctggtggtcg ttgacgatct tgccctgcct ttaggcgtta
    27361 ttcgcatacg ctccagaggt agcgatggtg ggcataatgg gctgaaaagt accaacgaaa
    27421 cgcttggaac acaggactac gccaggttgc ggtttggtat cggcaatggt tatccccgcg
    27481 gccatcaggt tgactacgtt ttgggtgagt ggagcgatga ggagctgaaa atcctccccg
    27541 aacgattggc taaggccgga gaggcaattt tgtgctttgg cacaataggc atagagcgca
    27601 ctatgaatct atataacaac cagtaagcta accaacaaat gggcgccgag agcgagcggg
    27661 ttgacaagtg gctgtggatg gtgagggttt ttaaaacccg ctccctggcc attgactgtt
    27721 gccgtaacga aagggtaacg gtgaacgggg tgcctgttaa agcctcccac accgttcagc
    27781 ttaacgactt ggtcgcggtg cgcaaacccc ctgttacgtt aacattcagg gtaaagggtt
    27841 tccccggtag ccgaattggt gcaaagctgg tggatgagta tgccgaaaac cttacccctc
    27901 cagaggagta tcagaagctc gatccgtcct tcagggcttt tagcatatac agaaatcccg
    27961 gaacaggccg gcctaccaag aaagagcgtc gttcgctcga cgactttttt gattcggaag
    28021 ccgatgattc cccttgggat gcggacgatt ccaactagat tactccactt tgaagcctaa
    28081 gttagcgcgt ggcgtttagt tgcccgaacc ggagcagtat atcttaacca tttgctctgc
    28141 aggctggtag cccagctcaa gtgctttccg gaaatcttcg cagccgccct tggtatcctt
    28201 cacgttcatg cgcgaaattc ccctgttgaa aaacgcttcg gcatccttag gatcgtattt
    28261 aatggcgatg tcgaaatcgg gtagggcatc cttatggttg cccataattg cttttgatat
    28321 agcccggtta tagtaagcct gagtgaagtt tttatcaata gagatggccc ggttgaagtc
    28381 ctcgattgac ccgcggtaat ccttcaaaaa agctttggca atcccacgcc cgtaaacagc
    28441 tttcaggtag tcgggcttta ggttgatggc cttgttgaac gactgaatag ctgctttaaa
    28501 atcgcccttg ttgagcaggg ctcctccgcg tgtgccatat atcagcggat cgttggggtc
    28561 tatcgaggca gccctgtcga ggtagccaat tgcctggtca aaatccttca ggttggagta
    28621 ggcctcaccc aggctgaaga gcagctcctt gtcgttggga aggtcgttaa gggcaaccag
    28681 caagtcggaa atcgctccat tgaagtcctg aattttcagc ttgaaggtgc cgcgcttttt
    28741 gtacgattcg ggggtggcgt tctttccctc aatcatcctg tcgtaatcag cgatggcgcc
    28801 cttaaagtcg gaaatgttgg ccagcgattc gcccctgctg tagaatgcct ccaggtttga
    28861 gccgtctagc tcaatgctgc ggttatagtc ctttaccgcc tggtcgtact ccatcatggc
    28921 tgcgttgagc aggccgcgct ggtagaaagc cctggagttg gagtcgttta atgaaataca
    28981 ccggttaagg tctctcagcg aggcggtgta gttgccaatt tttctaaggg aaacacccct
    29041 gtttaggaag tagtcaaagt tcatggagtc aatggcgata gctttggtgt agtcggcaat
    29101 ggcggcagag tcgcggttaa ggccggcaag cgccattccc cttccaaaaa ggtagtccgg
    29161 atttttcggg ctaaacctga cagccctgtt aagctgttcg taggcctgcc catactgcag
    29221 cgagtcgaga aggacgattc ctttttcgta gctattttgt gctattctgg gacggagcag
    29281 taggtgagct gctaccgcta ccactactat tccgattaca atatacaggg tagttttctt
    29341 cttcatctcc tttgtgttgt ttagttctac acgcactgac agcaagccta accactgcct
    29401 aaccggccca aatttagttt atttgactgg ttggaacaac cgcctgttaa attttttgat
    29461 gtaataagct taacatgtaa ccaaaggagg gtagggtgtg gtgtacactg gcgtccggaa
    29521 agggaatgca atcggctggt tatgtgtgcg ggaacgaatg tttatgtatt tatcgcaaaa
    29581 atgtaccgtg gcaatattta cggcaattta ttcaaattat cctccccatc gtttcggatt
    29641 gttaatgata tattgccgta tgcgggttaa ttcggtatca tcccggatga tgtgttcgta
    29701 ataatttcgt tgccacaatg gttgcattgg tattgtggag acgtgtggag acacgttgca
    29761 acgtgtctcc acacgtaatt ggttgatttg ttttgttgtt gttgatttga ataatcgtac
    29821 aatggttgga attgaatttt ttgtgggttt cccgaattgt tcggttgttt ttgttgtgga
    29881 ggcacgttgc aacgtgcctc cacaatcata attatccgtt ataattaaaa tcccgtgtaa
    29941 atgatttggc ataacaacga atgcatcaat aacgatattt gggcgaataa tgccggtttt
    30001 ctgccattcg gtttttacca attccccata ttcattcaa
//
LOCUS       DAOSHM010000030        29651 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_1219_length_29651_cov_20.980268, whole genome shotgun
            sequence.
ACCESSION   DAOSHM010000030 DAOSHM010000000
VERSION     DAOSHM010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 29651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29651
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1219_length_29651_cov_20.980268"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(488..1603)
                     /locus_tag="PK839_06260"
     CDS             complement(488..1603)
                     /locus_tag="PK839_06260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72377.1"
                     /translation="MVSKRIGHKPKIAAALALVQLLAIQQFCYGQDSTSTLGLNLYSS
                     AFFDNKEFTGDIKKGYTLPGFFIQPVINFSTDNYSVEAGFHLLYMAGTDSLEHLVPVL
                     TLARKLSKSSTILVGTLDARNGHFLPEPLFKSERVFTSQPETGVQLKHKTNNTFSDLW
                     INWEQFIKTGSPFQEQFTVGFTFQYLESNSKTPVFFTKTMALATHQGGQIDSTNLPVT
                     TIVNIGETAGILIPLKPGNANLSIEVSGFLSADKSPNPHLKYDQGYAFYPRIQMEWPF
                     TRFSFGYWHARSFINPRGEELFGSLSTVNPTLDSKVRKLVTCSLEYSKTLQQGFTFGV
                     GVNGYYDTRQGLLDYSYTFRMIFDGELVRFRRTGASR"
     gene            2455..3636
                     /locus_tag="PK839_06265"
     CDS             2455..3636
                     /locus_tag="PK839_06265"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008157124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HOG72378.1"
                     /translation="MKRKQKIQLIALAVLAFLAIFLSLPQNRYIVKALIYQQVNIDDY
                     KIFHNRTVRMGQPQPWKISPQYNHYQLSDKQLEYFERYKTVAYLVVKDTAILYESYWD
                     GYGSDSYSNSFSMAKSIVSLLVGCALQDGYIRSIDQPVADFLPEFRQDGRDKITIKHL
                     LTMSSGLSWDESYSTLFSLTTKGYYGNNLAGLVLGQHVVEEPGKRFMYRSGDTQLLSL
                     VVEKASGETLSDYASKRLWSKIGAEHDALWCLDRQNGVEKAFCCFNSNARDFARFGQL
                     VLNQGTWNGEQLIPKDYLAEALQPATYLIDAETGKPCDFYGYQWWRINMDGHEVSYAR
                     GLLGQYIFVIPSLNAVVVRLGHLRSDVTIEGQPEDVYQYLRCGVEIIKQLQIPSEYER
                     K"
     gene            3623..4825
                     /locus_tag="PK839_06270"
     CDS             3623..4825
                     /locus_tag="PK839_06270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72379.1"
                     /translation="MSENDNLFLTLERLRVKLLAFPAADVTLFEKAKKKHISLFNPEQ
                     IEFVNDKPDILMFLTGGSEHIAIESVQEYRFYLMLASREANAWASATEVKAWMNMHNV
                     SSLLINTDDPLASTYIQEFYKASNGIRHLHGQRLGVVGKPSAWLVASTISPFLLQSRL
                     GIEQVDISWNEIMFDEIQQVSPDFTTLFGMADDKEELMASGRIFEGLASLIPFYRLNA
                     LSVECFPLVNQTSHTACLALAKLNLDGFPTACEADSCSAAGMMLANEVCGVIPWMANT
                     ILVEGNRAVFAHCTAPLNLLEEFKLDSHYETGKGYSIAGSLRSPDVTIFRLDNTLNKI
                     FITRAEVKSRPKSKTACRTQLEVEISDSAARYFTDSPFGNHHLILPNSYTMRLKLAAR
                     LLRMEVVE"
     gene            complement(4903..5469)
                     /gene="frr"
                     /locus_tag="PK839_06275"
     CDS             complement(4903..5469)
                     /gene="frr"
                     /locus_tag="PK839_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007366899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HOG72380.1"
                     /translation="MNPNDATATISTADEHMQKAIDHLENELRVIRAGRANPAILSGV
                     MVDYYGTMTPLSQVSSVGTPDARTIVVQPWEKNMINPIEKAIMAANLGFNPQNNGESI
                     RIMVPPLTEERRKELVKQVKHVGETARMSIRNARRDAIEEVKRLQKNGLPEDAAKDAE
                     AKIQKITDSFNKKVDELLDKKEKEILTV"
     gene            complement(5501..6211)
                     /gene="pyrH"
                     /locus_tag="PK839_06280"
     CDS             complement(5501..6211)
                     /gene="pyrH"
                     /locus_tag="PK839_06280"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HOG72381.1"
                     /translation="MIKYKRILLKLSGEALMGADAYGISTDVLNSYARQIKEVTDLGV
                     EVGIVIGGGNIFRGLKGCSMGFDRVKGDQMGMLATVINSLALQSAIEGIGGKARVLTA
                     TKMEPVGELYSKDKAIQVLSEGNACIIAGGTGNPFFTTDTASALRAVEINANILLKGT
                     RVDGVYSADPEKDPSAHRFESITFTEAYDKKLKIMDLTAFTLCSENNLPILVFDMNTP
                     GNLKKAVFGDKVGTLVKN"
     gene            complement(6333..7160)
                     /gene="tsf"
                     /locus_tag="PK839_06285"
     CDS             complement(6333..7160)
                     /gene="tsf"
                     /locus_tag="PK839_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HOG72382.1"
                     /translation="MPNITAADVSKLRKITGAGMMDCKNALVEAEGDIDRAIELIRER
                     GKAIATKRAGREAGEGAGLAKVNANATRGAMIVLNCETDFVAKNEDFLKVAKKILDFA
                     LDTAPSNLEALKESVMEGRKVSDMITEFSGITGEKVELSYYDQVEAPMVVPYIHPGNK
                     LATIVGLSKVTDMQVAKDIAMQVAAMNPVSIDKEDVPEEVKLKELEIGREQARLEGKP
                     ENMLDKIAEGKLQKFFKESTLLNQEFIKDNKMTVRQYIQSADKDLKVLAFKRVSLNV"
     gene            complement(7184..8053)
                     /gene="rpsB"
                     /locus_tag="PK839_06290"
     CDS             complement(7184..8053)
                     /gene="rpsB"
                     /locus_tag="PK839_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HOG72383.1"
                     /translation="MSRTSFQELLDAGVHFGHLKRKWNPKMAPYIFMEKNGIHIIDLH
                     KTVVKIDEAAAAVKQIAKSGRKILFVATKKQAKDIVAERIKPINMPYVTERWPGGMLT
                     NFATIRKAVKKMSSIDKMMTDGTIDTLSKREKLQVSRQRAKLEKNLGSIADLNRLPAA
                     LFVVDVQKEYIAVREAKRLNIPVIAMVDTCCDPTPIDFVIPANDDASKSIALIVDIMA
                     KAIEEGLEERKAEKEKEPAQKEKSEEQPTRTRARKVKVKVESTHNEGNAEVADSVEEV
                     AEQNTSVEENEQE"
     gene            complement(8164..8550)
                     /gene="rpsI"
                     /locus_tag="PK839_06295"
     CDS             complement(8164..8550)
                     /gene="rpsI"
                     /locus_tag="PK839_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018360966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HOG72384.1"
                     /translation="MQIYNGLGRRKSAVARVYLREGKGNIVINGRDMKDYFQSELLQY
                     VVMQPLNVLNVGGNFDIKVTLDGGGVKGQAEAVRLGISRALVEYNPDNKPTLKANGFM
                     TRDSRSVERKKPGRPKARKRFQFSKR"
     gene            complement(8559..9014)
                     /gene="rplM"
                     /locus_tag="PK839_06300"
     CDS             complement(8559..9014)
                     /gene="rplM"
                     /locus_tag="PK839_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018697208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HOG72385.1"
                     /translation="MDTLSYKTVSANKATVTKEWVVIDATNQVLGRLSSQVAKILRGK
                     HKPNYTPHVDCGDNVIIINAEKVKLTGHKLTQKQYVRHTGYPGGQRFSTPKEMLQRKP
                     IWVVEHAVKGMLPKNRLASDLFRNLHVYEGPEHPHEAQKPRVLDLSTFK"
     gene            complement(9123..9308)
                     /locus_tag="PK839_06305"
     CDS             complement(9123..9308)
                     /locus_tag="PK839_06305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72386.1"
                     /translation="MEILLLLNNRSFSRGRISMYKMFDFGYGQIEENKEIMPEKCSTL
                     HFRNPNMDKNHLRGNLT"
     gene            9475..11772
                     /locus_tag="PK839_06310"
     CDS             9475..11772
                     /locus_tag="PK839_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOG72387.1"
                     /translation="MHSYLDELSESQREAVTTSEGPSLVIAAAGSGKTRVLTYRIAHL
                     LNLGVPPWSILALTFTNKAAREMKERIAKIVGDSDAHKLWMGTFHSIFARILRSEAEL
                     LGYSKNFTIYDTTDSRNLIVQIIKELGLDSQVYKPGEVLSRISSAKNNLVTPQAYAAN
                     SSIIAQDVKARKSEMSRLYSLYTQRCFKSSAMDFDDLLLNINILFRDFPDVLLRYQNR
                     FRYILVDEYQDTNFAQYFIVKKLSEKYRNVCVVGDDAQSIYSFRGAKIENILNFRNDY
                     PDYKLFKLEQNYRSTQTIVNAANSVIENNQKQIKKVAFSANEVGDKIKLIKAYTDQEE
                     GFLVASEILNTIRDKSCAYSDIAILYRTNAQSRIFEETLRKRNIPYRVYGSLSFYQRK
                     EIKDLLAYFRLSVNPNDDEAFVRIVNYPARGIGSTTLQHLQELANVQNISLWETAQQL
                     KQLNHQLKPAAVKKLTDFVGMVGGFQSAVYTADAYEAAYSIARNSGILTDLKADKTQE
                     GLSRLQNIEELLNSIREFIEARRNEGVDETTTLVDYLENVALLTSLDNDDPNDRNRIS
                     VMTIHSAKGLEFDYVFLTGLEENLFPSNMSMNTPEELEEERRLFYVALTRAAKGVTIS
                     FAQTRLKWGNINISNPSRFISEIDQRYVESPFEPDGGGHLKATSELSRQTSINNFEKK
                     SIGNRAAPYSQPPGRTPVSVPENLKELPPEEICEGVEVYHGKFGQGKVLSVEGEGANK
                     KATVLFTEAGQKNLLIKFANLKGIA"
     gene            11985..12569
                     /locus_tag="PK839_06315"
     CDS             11985..12569
                     /locus_tag="PK839_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4290 domain-containing protein"
                     /protein_id="HOG72388.1"
                     /translation="MDYNTQRKKLALPEYGRHIQQMVDYIASVEDRDERNRLAKSLVA
                     IMGNLNPHLRDINDFKHKLWDHLFIMSDFKLDIDSPYPIPSMEQYNEKPRRVPYPGNP
                     IKFKHYGRIIEMMIEKAVAMEEGPEKDALKQLIANQMKKANITWNKESVSDDDIFRDM
                     QTLSNGMLVMAPGSKLVDNKEFKNKPRTKYHKKH"
     gene            12576..13886
                     /gene="murA"
                     /locus_tag="PK839_06320"
     CDS             12576..13886
                     /gene="murA"
                     /locus_tag="PK839_06320"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HOG72389.1"
                     /translation="MATFEVIGGKRLKGDLFPQGAKNEALQVICATLLTPEKITINNI
                     PAIRDVVKLIELLQGMGVEVTRLSESSYSFIARNIDIDYLTTDDFKVKAAAIRGSIMV
                     VGPLLARYGKAYAPKPGGDKIGRRRLDTHLLGFQKLGVEFDFDVNDNFFTLEGKHLKG
                     SYMLLDEASVTGTANILMAATMTPGKTTIYNAACEPYVQQLCHMLVSMGARISGIGSN
                     LLTIEGVDGLGGCEHTILPDMIEIGSFIGLAAMTCSDITIRNVSFGNLGIIPDAFRRM
                     GINFEVTNDDIHIPPHEHYEIDTFIDGSILTLADAPWPGLTPDLLSVFLVVATQAKGS
                     VLIHQKMFESRLFFVDKLIDMGAQIILCDPHRATVIGHDRQLRLRPTVMTSPDIRAGV
                     ALLIAAMSADGKSIIHNIEQIDRGYQNIDGRLNAIGAEIHRIDR"
     gene            complement(14148..15122)
                     /locus_tag="PK839_06325"
     CDS             complement(14148..15122)
                     /locus_tag="PK839_06325"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOG72390.1"
                     /translation="MGMSNKKFLVYIAVTVAMAFWALSFIWYKDAYVYYRPITLVFFR
                     LVISAAFLYVFSLAMRKLERVKKGDLKLFFLLAFFEPLLYFIGESFGMTLISSTMAAV
                     IVSTIPLLSPVGEFFMFHRRISTTNLMGIIASMLGVCLVIFHNGFGQVDVNFTGVALM
                     FLAVFSTVGYSLIVGKLTVKYNVYSIITYQNTIGALYFMPLFLIFDLNHFQTVGISWQ
                     AMVPVIKLGIFASTFAFMLFTYSIKHLGITRANTFSNAIPVLTAIFAYFLLDEALTPV
                     MIAGILVVVSGLFLSQISPKTKTQIELKVLPEHPVVGIGLRLRKLFRK"
     gene            complement(15410..17713)
                     /locus_tag="PK839_06330"
     CDS             complement(15410..17713)
                     /locus_tag="PK839_06330"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG72391.1"
                     /translation="MNSTEFHRNILLVLILLTFSLSYSQAQSDTVRIGEMLQRAEVLR
                     TTNPDSAIGLAIQALNIIDGSDNKELMVRVEHTLGNCYYSRNDFELALSHLKKSLQLA
                     TEEGRKFWVAMNLNRIGNVYQLKSDYGSALQAYTEALNINRSIDNKSQLARTLVNIGT
                     VYSITGSYKEAIENMLKALETYEQAKDYEGIAWASLSISRLFSRIDLYEKALQYAESS
                     LTHYRQLSNINGVTLAKTELANIYYHLGMYPKAKEMATDVLATNQQNQNIYGQAANHL
                     LLGIINFELGDYTSAKSNLLTSLELKRQVNDSIDLAKLSRYLGEVNLKSGNVAAGLES
                     LEQAMRIAQRQRVLTDQRDIFLSLSQAYRLRGDYRRSLDYFTRYSAVKDSINASQIAR
                     MEMQYDFDKREREQELISRQREAIQQAKLERQRMLTLFISIALLLAVALAGVIFYFYR
                     NKKKINALLVAQNQEILRQKEEIEAQKEEIESQRDLAERQRDQIAGQQKQITDSIRYA
                     SRIQSAVMPRTDTLATLLTDYFIYFQPKNIVSGDFFWVTRLNDGRIAIAVADCTGHGV
                     PGAFMSILGITLLKEIIAYQQVNTAGDLLDKLRQMVIAALNQSGQVQDSHDGMDMGLL
                     VVDAKGRKAEYAGAYMPLLVSRNSELPEVSAGVQPETANGWSIYEIKGDKMPVGYHLA
                     GERPFTTHRFDLLSNDTFYLMSDGYTDQFGGALNQKLMMVNFKKNLLGLQTLSLSRQN
                     EELQKQLIEFQGTQKQVDDILVMGFRL"
     gene            17920..19281
                     /locus_tag="PK839_06335"
     CDS             17920..19281
                     /locus_tag="PK839_06335"
                     /inference="COORDINATES: protein motif:HMM:NF017509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HOG72392.1"
                     /translation="MNVVKENTNEQTISIRARVEQADYVEKVEKKLKDYRKNSNIKGF
                     RPGMAPAALIHRMYYKYVLVDEVTHIAFDAVMDYIDKEKIKILGEPLPSEGQNTINWD
                     TDKEFELAWEIGVAPEFEIKLGKKDKIPFYNIAPDDKIRKQYIDSYLMRFGHFAEADE
                     TNERGLIEATLTELNSDETPREGGLFVERATISVELVADPDERAKLVGVKVGDVLTID
                     VQKAFPNEADRASLLSTQKENLPNIQPVFQLTVNKTSNYQPAELNQELFDRVFGEGVV
                     TTEEEFYQKIDEEIESGLKNEGENRFAVELRNILLNKIDMQLPKDFLIRWLTTINEGK
                     FTREQVENEYPLFEGDLKWQLIRNKIASEQNFTVTNEEMESFAMEYAQQQFMAYGMSY
                     LPKEYIERYAKDILKNQDEIRKMQEGIISRKVIAWFKENLPLDVKEITHEEFDKLEPV
                     SGK"
     gene            19408..20091
                     /gene="clpP"
                     /locus_tag="PK839_06340"
     CDS             19408..20091
                     /gene="clpP"
                     /locus_tag="PK839_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010266217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HOG72393.1"
                     /translation="MNEFEKYATKHRGISSLSLQRFGTITNSYISPTIIEERQLNVAT
                     MDVFSRLMMDRIIFLGVPIDDDVANIIQAQLLFLESTDPSKDIQIYINSPGGSVYAGL
                     GIYDTMQYITSDVATICTGMAASMAAVLLTAGEKGKRTALPHSRVMIHQPMGGTEGPA
                     TDIEITTREILKLRDELYEIIATHSGNPIDRIRKDSDRDYWMTAREAMEYGMIDEVLT
                     RSKNLKETK"
     gene            complement(20129..20956)
                     /locus_tag="PK839_06345"
     CDS             complement(20129..20956)
                     /locus_tag="PK839_06345"
                     /inference="COORDINATES: protein motif:HMM:NF014305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOG72394.1"
                     /translation="MLNQSVKKFWKKLPRLCLIGMVGIMPSINKVVGQGFESLYEAKV
                     FTHKGKTLPYRIMYPKNFNPEQKYPLVIFLHGSGERGNDNQKQLVHGASLLANDSVRS
                     RFPAIVVFPQCPEDDFWANIKENPNQQGYRSIEFLPESKPTAAMDLLIRLVNGLKRES
                     YVDSRRIYVGGLSMGGMGTFELVARMPKVFAAAFPICGGSNPESAKRYAHKVKFWVFH
                     GAKDDVVPPQYSIDMVSSIRKLGGDARLTVYPDAGHNSWDQAFREPELLLWLFSCKK"
     gene            complement(21021..21719)
                     /gene="radC"
                     /locus_tag="PK839_06350"
     CDS             complement(21021..21719)
                     /gene="radC"
                     /locus_tag="PK839_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007755017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HOG72395.1"
                     /translation="MEQEYKRLTIKDWAVEDRPREKMLHQGIGSLSDTELIAILLRSG
                     NPEETAVELAQRILNMAQNNLNELGKFTIHQLTQVKGVGEAKAITLLAALELGRRRKS
                     VEAINREQITSSRNVMEIFQPLLADLPHEEFWVLLLSRANKVLDRVRVSQGGVTGTVT
                     DVKLIIRAAVEKLASAIIVVHNHPSGNPKPSEKDIAITEKLKQAVDFFDIALLDHLII
                     TDSKCYSFADEGTL"
     gene            complement(21999..22982)
                     /locus_tag="PK839_06355"
     CDS             complement(21999..22982)
                     /locus_tag="PK839_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOG72396.1"
                     /translation="MIPSSSIFSLKPDGDFEKIALEVFRFQSQHVRVYHDYIQQLGVN
                     PDAVASTDEIPFLPIQLFKTQDVIAEACQPEATFTSSGTTGNEVSRHRVADLSLYRES
                     FTRTFSIFYGSPSDYCILALLPSYLERSGSSLVFMVDELIRQTGNPDSGFYLNQLYAL
                     TEKLSALDRSGQKTILIGVSFALLDLAERFTFNLQNTIIMETGGMKGRRKEVVREELH
                     STLCSAFGVNVIHSEYGMTELLSQAYSTGKGMFRCPPWMRVVIGSTDDPFCLVGDGRT
                     GIIKIIDLANLYSCSFIQTQDMGKLHSDGCFEVLGRMDDAPIRGCNLLVYD"
     gene            complement(23092..24354)
                     /locus_tag="PK839_06360"
     CDS             complement(23092..24354)
                     /locus_tag="PK839_06360"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005862970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOG72397.1"
                     /translation="MITIDSYSFKGKKAIVRVDFNVPLDKKTLEVTDDTRIRGAVPTI
                     KKILGDGGAVILMSHLGRPDGTPQDKYSLRHVVPAIEKNLGQKIKFAADCMGEETKKM
                     AAGLKPGEVLLLENLRFYEEEEGKPYSLPETASEEEKKEAKARVKESQKNFVKQLASY
                     ADCYVNDAFGTAHRAHASTALIAAHFPGNSMFGYLIESELKAMDKVLKSPTKPFTAIM
                     GGAKVSDKILIIENLLDRVDNLIIGGGMTYTFICAQGGKVGKSICETDKLELANQLLE
                     KAKAKGVKVYLPVDAVNADAFDANANTQITPIDSTPDGWMGLDIGPKTIAKFREVILE
                     SKTILWNGPMGVFEMDKFAAGTSEIAKAIAEATQRGAFSLVGGGDSVAAVNKNHLADK
                     ISFVSTGGGAMLEYMEGKELPGIKAIRG"
     gene            complement(24787..25254)
                     /locus_tag="PK839_06365"
     CDS             complement(24787..25254)
                     /locus_tag="PK839_06365"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOG72398.1"
                     /translation="MEIRQAQKQQLIEVLYIIRECSNQLVEKGVNFWSNSHADMEEIS
                     SDIEQGYVYVLVNNHVSIATVTLKPHATNQKVMTISRLAVFPAFQRKGLASMLISFGI
                     DLAQKGNFTAMEGYSPLYDSNLAALLEKRGFERKGTLSPPSEKIGSIIFEKLL"
     gene            complement(25244..26896)
                     /locus_tag="PK839_06370"
     CDS             complement(25244..26896)
                     /locus_tag="PK839_06370"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOG72399.1"
                     /translation="MAKNWYVYLLLTVTLMACSKREKADLIVTNAKIYTVDSTFSTHS
                     SMAVVNGIVKELGDSVYIMSKYTAKKVVDAGGMYVYPGFIDSHCHFLGYGLSLNKAML
                     SEASSWDEVVARLVEHQKRFPTSWVEGRGWNQNEWKVREFPTNELLNRAFPDKPVYIV
                     RIDGHAAIANNAALKIAGVTASTRVEGGEVVLQKGKPTGLLLDNAMELVRRIIPKPTI
                     DEKAAALQNAQKKCFAVGLTGVQDAGLDVDEVLLIDSLQQSGKMEIWVNAMLNPTPAN
                     YERFISKGVYRTNKLTVRSIKLYADGALGSRGALLLEPYSDDRGNRGLQVEPTEKLDS
                     LCGMAFKAGYQVCTHCIGDAAVKLMLDIYSKYLPAGNILRWRIEHAQVVDRNDLPRFG
                     SLAVIPSVQTTHATSDMDWADERLGSRIKNAYAYQELLLQNGWIANGSDFPIESINPL
                     YGFYAGVARKNLEGKPDGGFQPENALTRKQALCAMTIWAAKAAFCEDARGSLEPGKAA
                     DFVILNTDLMNDELPAIPRATILKTFVSGKEVFGSNAFSHGN"
     gene            27031..28476
                     /locus_tag="PK839_06375"
     CDS             27031..28476
                     /locus_tag="PK839_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72400.1"
                     /translation="MAKLPTACLLAVASIFALCHSACVRDDFSESSALKLSFSADTVL
                     FDTVFTTIGSSTQYFKVYNRSGHDLHISSIRLAGGSTSPYRINVDGRSGVTFSNVSLR
                     SRDSLFVFVEVTVDPTSLNSPLLVADSIMFSTNGNIQDVKLVAWGQDVHLLRSEIISN
                     DTVLATDKPYLIYSYLLVDTATTLTIPAGVRMYFHNQAMLVVAGTLKVEGEQGSPVVM
                     EGDRLEDFYRDKAGQWGGIWLTAGSRYSAISWAEIRNAITGITVDTCFTSHAPTLTLS
                     HTRIENCSYVGLLARGAKVKADNCLVSNAAQVCVALTMGGEYRFYHCTVANYWGDYIY
                     RKGPALLLNNYYLYRLEENGPLYVEPRDLVEASFFNSIVYGSMSSEFQVDNIVNGAPV
                     AAQMNYHFEDCILKVPQNFDLTDTEKFTRVFTLDPKFKDSNAHVFLLDTLSPAMGIAN
                     PAVATDYPIDLNGFNRLTDGKPDLGAYERQE"
     gene            28478..29515
                     /locus_tag="PK839_06380"
     CDS             28478..29515
                     /locus_tag="PK839_06380"
                     /inference="COORDINATES: protein motif:HMM:NF040322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HOG72401.1"
                     /translation="MRKLLIILPILLACAASTKVKAQNIPATRHRVMFYNVENLFDPF
                     DDSLTLDEEFTPFGDRHWTWKKMEQKLNGIYKVILAVGEYDPPVLVGLCEVENGFVLH
                     QLVSQTPLSKFEYRVVHRESPDRRGIDVALLYRPDRFTLQEKEFVTVRFPDEPWRTTR
                     EILYVRGLLGGDTLHVFVNHWPSKYGGELESEGGRYAAAYALKNKVDSIKIFYPDARI
                     LIMGDMNDEPESDPVARGFGACLTAETACPYDFVNLSAILKQRGQYSYKYQGVWGIID
                     QLIASRSMLDSRHVLYTFTGNAGVFSAPFLLEDDERYAGKRPFRTFIGFKYHGGYSDH
                     LPVYLDLFSNK"
BASE COUNT         7524 a         6935 c         6985 g         8207 t
ORIGIN      
        1 cagtgccgct tgggtattcc cacgctacgt tccgcccccg acccatttgc gggtctggtt
       61 tttatgcctt ttttataata ccagcaacgt tcaactattc ccgcctttcc gagcactgcg
      121 gagaatacct tctccccagt aagggctgaa gacaaggata aaggataagg gttaaaggat
      181 aaaaaaaatg tttgggtaac gatgaagcgt gtccgcctcc cggcggaact tgtccgcctc
      241 ccggcggatt catggaagct attccgcccc ttcagggctt aacgatgttg gaaacgtcct
      301 tatcaagggg ctttgcccct tgctgcttat tgcaccccgt tggggctgaa gaatgaaaaa
      361 gcaaagaata ttttcaagtt ttgacggact taaaacaatg acaggctttc gtggaatatt
      421 aagttcaacg ctaaatttcg actgttaaca attaacaggt tcagttctaa cgggtctttt
      481 cgagttgtca acgagatgcc ccagttctcc tgaaccgtac tagctctccg tcgaaaatca
      541 ttctaaaggt gtagctgtag tccagcaatc cttgcctggt atcgtagtag ccatttactc
      601 caactccaaa ggtaaaaccc tgttggaggg tcttactgta ctccagcgaa caggttacga
      661 gcttgcgaac ctttgaatcg agtgtagggt taacagttga cagtgaacca aaaagttctt
      721 cacctctggg attaataaat gatctggcat gccagtaacc aaatgaaaag cgggtaaaag
      781 gccattccat ttgaatcctt gggtaaaacg cgtagccttg atcatattta aggtggggat
      841 ttggcgattt gtcagccgaa agaaaacctg aaacctcgat gctaagattg gcattgcccg
      901 gtttgagagg aattagtata cctgccgttt ctccaatgtt aacaattgtg gtaaccggaa
      961 gattggtgct gtctatctga ccaccctggt gggtagccaa tgccattgtt ttagtaaaaa
     1021 agacgggggt cttactgttt gattccagat actgaaaggt aaatcccacc gtaaactgct
     1081 cctgaaaagg gctacccgtt tttatgaact gctcccagtt aatccacaag tcgctgaagg
     1141 tattgttcgt tttatgctta agctgaaccc cggtttcggg ttggctggta aacacccgtt
     1201 cggacttaaa aagtggttca ggaaggaaat ggccatttcg ggcatccagt gtgccaacca
     1261 ggatggtaga gcttttggaa agctttcgtg caagtgtcag gacagggact aaatgctcca
     1321 ggctatcggt accggccata tatagaaggt gaaaacccgc ttcaacgcta tagttatcgg
     1381 tactgaagtt aataacgggt tgtataaaga atcccggcag tgtgtagccc ttttttatat
     1441 cacccgtgaa ctccttgttg tcaaaaaagg ctgaggagta aaggtttaag cctagtgtag
     1501 aggtgctatc ctgtccgtag caaaactgtt gaattgcaag tagctgcacg agagctaacg
     1561 cagctgcaat ttttggctta tgaccaattc gtttgcttac catgatgaat ataaaagttt
     1621 atctaattga aaatcgggta aaggtatgct gttttttgct agtttttcct agttttattg
     1681 ccctgttttc agcatttttt tgcaacaccc ttatatgtat ttgattatca atattaacat
     1741 tgtgattttg ggcgttgcaa aatttttatc atgtttatca gaaaagttaa atatcggtct
     1801 ggaagtcaat ctgtacaagt aatacaaaag tttcgagata actacaaagt tgtcaaaact
     1861 atcggttgcg ccaccactca gcacttaatt gaaaagctgg agcaattgac cagggaggag
     1921 gtagagcatt tgagtgggaa tcaaaggaat cgcatagaag ctcatatctg cattgctttt
     1981 gaggcctact gaatactcaa ggaacttgaa agagtcctac gggaagagaa atcgtccatg
     2041 tcgttaaaaa gatccagtga gttgacacga aacatgtatc atatcaccta tactttgccc
     2101 aaatcaaagc aaacaaaatc caaacttctc aacatggatg aagagcaatc tgaactctac
     2161 caaattattt gtaaaaattt tcagggtgtt gcaatgcgga aaacaggaaa agtttatcta
     2221 attgaaaatc gggtaaaggt atgctgtttt ttgctagttt ttcctagttt tattgctaat
     2281 ttgatataat ccgattttga ctatctttaa ggtttacaac tgtttagatg atgatgctta
     2341 taggttgagg cagtttggtt cttctagctg ttagggttta tcatcttatt ataggtaaac
     2401 tccttaggga cggacgattt ttttaacatt gaccataaca ctaaaagcaa acccatgaag
     2461 cgcaagcaaa aaattcaact tattgcgttg gcggtgctgg cctttttggc catttttctt
     2521 agcctacccc aaaatcgcta cattgttaaa gctttaattt accagcaggt aaatattgac
     2581 gattataaaa ttttccataa ccggacagtc cgtatggggc aaccgcaacc atggaaaata
     2641 tctcctcagt acaaccacta tcagctatcg gataagcagc tggagtactt tgagaggtat
     2701 aaaactgttg cctaccttgt ggtgaaagat accgccatac tttatgagtc ttactgggat
     2761 ggttacggct cagattccta ctccaactca ttttctatgg ccaagagcat tgttagcttg
     2821 ctggtaggtt gcgcccttca ggatggctac ataaggagta ttgaccagcc ggttgccgat
     2881 tttcttcccg aattccgtca ggatggccgg gataagatta ccattaagca tctacttacc
     2941 atgagctcgg ggctaagctg ggacgagtcg tacagcacct tattttcttt aaccacaaag
     3001 ggttactatg gaaacaacct tgcaggactg gttcttggac agcacgtggt tgaggagccc
     3061 ggtaaacggt ttatgtaccg tagcggcgac acccagctgc tatcgttggt tgtagaaaag
     3121 gcttcagggg aaactctttc tgactatgct tcaaaacgtt tgtggtcgaa aatcggtgca
     3181 gagcacgatg cactctggtg cctcgacagg caaaacggtg ttgagaaggc cttttgctgt
     3241 ttcaactcga atgcccgcga ttttgcccgc tttggccaac tcgtgctcaa ccaggggact
     3301 tggaatggag agcagctaat cccaaaggac tatctagcag aagcgctaca acccgccacc
     3361 tatctgattg atgccgaaac cggaaaaccc tgcgattttt acggctacca gtggtggcgt
     3421 attaacatgg atgggcatga ggtaagctac gcccggggtt tgctgggtca gtacatattt
     3481 gtaatcccca gccttaatgc ggtggttgtt cgcctaggtc acctccgaag cgacgtaaca
     3541 atcgaagggc aacccgagga tgtttaccaa taccttcgtt gcggagttga aatcatcaag
     3601 caattacaaa taccaagcga gtatgagcga aaatgacaac ctgttcctga ctttggaacg
     3661 gctaagggtt aagcttctgg cctttcctgc agctgacgta acgctatttg aaaaggctaa
     3721 gaaaaagcat atatcccttt ttaaccccga gcaaattgag tttgtgaatg acaaacccga
     3781 tattttaatg tttcttaccg gaggttctga gcatattgct attgaatcgg ttcaggagta
     3841 ccggttttac ctcatgctgg cttcacggga ggccaacgcc tgggcgtcgg caacggaggt
     3901 taaggcgtgg atgaacatgc ataatgtttc atccttgctt ataaacaccg atgacccgct
     3961 agcttcaacc tacatccagg agttctacaa ggcatcaaat ggaatacgtc atttacacgg
     4021 gcagcgtctt ggagtagttg gcaagccgtc agcatggctt gttgcttcta cgataagccc
     4081 ttttttactt cagagcaggc tgggtataga gcaggttgac atttcgtgga atgaaatcat
     4141 gttcgatgaa attcagcagg tgtcgccgga ttttacaact ctgtttggca tggctgatga
     4201 caaggaggag ctcatggcca gcggaagaat attcgagggg ttggcttccc ttatcccctt
     4261 ttataggctt aatgcccttt cggttgagtg ctttccgctg gtaaaccaaa ccagccacac
     4321 agcatgttta gcccttgcca agcttaatct tgatggattt cctacggcat gtgaggccga
     4381 tagctgtagt gctgctggaa tgatgctggc taacgaggtg tgcggggtaa tcccctggat
     4441 ggccaatacc attttggtcg aaggtaatcg ggcggttttt gcccactgca ctgcacctct
     4501 caacctgttg gaggagttca aactcgattc acactatgaa acgggcaaag ggtattcaat
     4561 tgccggtagc cttaggtctc cggatgtaac tattttcagg ttggacaaca ccctgaacaa
     4621 gattttcatc accagggcag aagtaaagag cagaccaaaa tctaaaacgg cctgtagaac
     4681 ccagctggag gttgaaattt ccgattcggc tgcccggtac tttaccgatt caccctttgg
     4741 aaaccaccac ctgattttac ccaacagcta caccatgcgg ttaaagctgg ctgcccgact
     4801 tttgagaatg gaggtggtag agtagcccaa aagggctatg aacttagagt ttggcagaaa
     4861 cagaaaagta atcttatcgg acaggtcaag tggtagggct ggctaaacgg taagaatttc
     4921 cttttccttc ttatcgagga gctcatcaac ctttttgttg aaggaatcgg taattttttg
     4981 aatcttggct tcggcatcct tagcggcatc ctcgggtaac ccgtttttct gtaaccgctt
     5041 cacctcctca atggcatcac gccgggcatt gcgaatgctc attcgggcag tttcgccaac
     5101 gtgcttaacc tgtttaacaa gctcctttcg gcgttcctcg gtgaggggtg gaaccatgat
     5161 gcgaatgctc tcaccattgt tctgggggtt aaaccccagg ttggctgcca tgatagcctt
     5221 ttctattggg ttaatcatgt ttttttccca tggttgcact acaattgttc tcgcatcggg
     5281 ggttcctaca ctcgacacct gagatagggg cgtcattgtt ccgtagtagt ctaccataac
     5341 tccagaaagt attgcggggt tagccctgcc ggcgcgtatt acccgtaact cgttttcaag
     5401 gtgatcaatg gctttttgca tatgttcatc agcagtgctg atggttgctg ttgcatcgtt
     5461 ggggttcata cacttactgt taattttatt gtaaaagtat ttaattttta actaaagttc
     5521 caaccttatc gccaaagaca gcttttttta agtttccagg ggtgttcatg tcaaaaacca
     5581 ggatggggag gttgttttcg ctacagaggg taaaggcggt caaatccata atttttagct
     5641 ttttatcgta tgcttcagta aaagttatgc tctcgaaccg atgggcagag ggatcttttt
     5701 cggggtcggc cgagtaaacg ccatccaccc gggttccctt taaaagaatg ttagcgttga
     5761 tttcaactgc ccgaagtgcc gaggcggtgt cggtagtaaa aaaggggttt cctgttcctc
     5821 cggcaataat gcaggcattc ccttcggaaa gcacttgtat ggctttgtct tttgagtaga
     5881 gctcacctac gggttccatt ttggtagcgg tgagcactct tgcttttcca ccaatacctt
     5941 ctatggccga ttgaagtgcc aggctgttaa taacggtggc caacatgccc atctggtctc
     6001 ctttcacccg gtcgaaaccc attgaacatc ccttaagccc acgaaaaata tttccaccac
     6061 ctataactat tccaacttct acacccaaat cggttacctc cttgatctga cgcgcatacg
     6121 agttaagtac gtcggtgctt atcccatagg cgtcagcacc cataagggct tcaccactga
     6181 gcttaagcag aattctttta tacttaatca ttttgatagt attatcggga tgatgatgta
     6241 aaggtacaaa aaaagaattt tacccgtaag ggtcagggta acataaaaaa accccgtggt
     6301 ataacacgag gttttaatgc tttgatgttg tgctaaacat tgagcgatac ccgtttaaag
     6361 gccagaactt tcaaatcctt atcggcgctc tggatgtact ggcgaacggt catcttgtta
     6421 tccttgatga actcctggtt cagcagggtg ctttccttga aaaatttctg tagtttaccc
     6481 tcggcaatct tgtcgagcat gttttcgggt tttccttcaa ggcgtgcctg ctcgcggcca
     6541 atttcaagtt ctttcagctt gacctcttcg ggaacatcct ccttgtcaat tgaaacaggg
     6601 ttcatagcgg caacctgcat ggctatgtcc ttggcaacct gcatgtcggt aaccttgcta
     6661 aggccgacta tggtggccag cttattgccc gggtgaatgt agggaactac catgggggct
     6721 tcaacctgat cgtagtagga gagctccact ttttccccgg taattccgga gaattcggta
     6781 atcatatcgg aaacctttcg gccttccata acagattcct taagcgcttc gaggttcgat
     6841 ggagctgtat caagcgcaaa gtctagaatt tttttggcta ccttaaggaa gtcttcattt
     6901 ttagccacaa agtcggtttc gcagttgagt acaatcattg caccacgggt agcgttggca
     6961 ttcacttttg ctaaaccagc accctcacct gcctctctgc ctgctctttt ggtggcaatt
     7021 gcctttcccc gttccctaat aagctcaata gccctatcaa tatctccttc agcttcaacc
     7081 agggcattct tgcaatccat cattcctgct ccggttatct ttcgtagttt ggagacatct
     7141 gctgcagtta tatttggcat atattttccc tttttagaat ttactactct tgctcgtttt
     7201 cttcaacgct tgtgttttgt tcggctacct cctcaacaga atcggcaact tcagcattcc
     7261 cctcattgtg ggttgattca accttaacct ttaccttgcg ggcacgggtt cgggtgggct
     7321 gttcttcgct cttttccttt tgggctggct ctttttcctt ttcagccttt ctttcctcaa
     7381 gcccttcctc aatagctttg gccataatgt caactatcag ggcaatggat ttggaagcat
     7441 cgtcgttagc cggaattaca aagtcaatgg gagtaggatc acaacaggta tcaaccattg
     7501 caattaccgg tatgttaagc cgcttggctt cacgaacagc gatgtattcc ttctgaacat
     7561 ccactacaaa aagggctgct ggcagcctgt tgaggtcggc aattgagccg aggttctttt
     7621 ccagcttggc tcgctgacgc gaaacctgaa gtttttcacg ttttgatagg gtatcaatgg
     7681 tgccgtcggt catcatcttg tcgatggagg acattttttt aacggcctta cggatagtgg
     7741 caaagttggt gagcattccg cctggccagc gctccgtaac atagggcatg tttattggtt
     7801 taattcgctc ggccacaata tccttggcct gcttcttggt agcaacaaag aggattttgc
     7861 gtcccgattt ggcaatttgc tttactgcag cagcagcctc atctattttt actacggttt
     7921 tgtggagatc gatgatgtga atgccattct tctccatgaa gatgtatggc gccatcttgg
     7981 gattccactt gcgttttaga tggccaaagt gcactcctgc atcgagcaac tcctgaaatg
     8041 atgttcttga catggttgtt ttttagttta gattaattgg caatcgcaca gtagtcccgt
     8101 gggggagtct gggcaattta tcactaaact gcttgccaaa ctactatggg aaaatttata
     8161 attttaacgt ttgctgaatt gaaatctctt tctagccttt ggcctaccgg gtttcttgcg
     8221 ctcaacgctg cgtgaatcgc gtgtcataaa cccatttgcc ttaagggtgg gcttgttatc
     8281 ggggttgtac tcaaccagcg ctctggaaat acccagcctt actgcttcgg cttggccctt
     8341 aacaccgcca ccatcaaggg taaccttgat atcgaaattt ccaccaacat taagtacgtt
     8401 aagtggttgc ataacgacgt actgcagcag ctccgattga aagtagtcct tcatatcccg
     8461 gccgttaata acgatgttac ctttgccctc gcgcaggtaa actctggcta cagccgactt
     8521 tcttcttcct aatccgttgt aaatctgcat accttaaact atttaaaagt gctcaaatct
     8581 agtactcttg gcttttgtgc ctcgtgcgga tgctcgggac cctcatatac atgtaggttc
     8641 ctgaacagat ccgatgcaag cctgtttttt gggagcatac ccttaacagc atgttcaaca
     8701 acccaaatgg gcttgcgctg aagcatttcc ttcggggtcg agaatcgttg tcctccggga
     8761 tagcctgtgt gtcgtacgta ctgtttctgg gtaagcttat ggccggtgag cttaaccttc
     8821 tcagcattaa tgataataac gttgtcacca cagtcaacgt gtggcgtgta gttgggtttg
     8881 tgcttgcctc taagtatctt ggcaacctgg ctcgacaacc tacccagaac ctggttggtg
     8941 gcatcaatca caacccactc ttttgtaacg gttgccttgt tggccgaaac cgtcttgtag
     9001 cttagtgtat ccactgtaaa ctcctagttt taagttaata cctgttgttt tgtgatcctt
     9061 gcatttttaa acatcaagtg ggttgcaaag gtataactat ttatttaaac cacaacacct
     9121 ttttatgtta agtttcccct taaatggttt ttatccatgt ttgggtttcg aaaatgcagg
     9181 gttgagcatt tttcgggcat aatttctttg ttctcctcaa tttggccata accaaaatcg
     9241 aacatcttgt acatggaaat tcggccccgg gagaagctgc ggttgtttag tagaagtaaa
     9301 atttccaccg tcccgatgtg ttttccttca atcgtctatt cttctaaaac tgttccttcg
     9361 gtgctgatat tatgagactt ataattatcg aagctctttc aaccgatgtt ttgccggtca
     9421 ttttttgtat agccattttt caggactatt tttgcaggtt gaaaatacca caccatgcac
     9481 agctaccttg acgagttgag cgaaagccaa cgtgaggctg ttactacttc cgagggtccc
     9541 agcctggtta ttgcggctgc cggatcgggt aaaacaagag tacttaccta ccgaattgcc
     9601 catctgctca acctgggcgt gcccccctgg tcgatacttg ccctgacctt taccaataag
     9661 gccgcgcggg agatgaagga acgtattgcc aaaattgttg gggattccga cgcccataag
     9721 ctctggatgg gaactttcca ctcaattttt gctcgtattc tccgctccga agcagagctc
     9781 ctgggctaca gtaagaattt caccatttac gatactaccg actcccgtaa cctgattgtt
     9841 caaatcataa aagagctggg gctcgacagc caggtttata agcccggcga ggtgctgtcg
     9901 cgtatatcct cagctaagaa taacctggtt accccgcagg cctacgccgc aaactcaagt
     9961 attattgcac aggatgtgaa agcccgcaag tcggaaatga gccgtttgta ctccctttac
    10021 acccagcgct gttttaaatc gtctgccatg gattttgacg atttgctgct aaacataaac
    10081 attcttttcc gcgactttcc ggatgtgctg ctgcggtatc agaacaggtt ccgctacatc
    10141 ctggttgacg agtaccagga taccaacttt gcccagtact tcattgtaaa aaaactctcg
    10201 gagaagtaca ggaatgtttg cgtggttggc gatgatgcgc aaagtattta ctccttcagg
    10261 ggcgctaaaa tcgaaaatat cctcaacttc cggaacgatt accccgacta caagctcttt
    10321 aagcttgagc aaaactaccg ttctactcaa accattgtta atgctgctaa cagcgttatt
    10381 gaaaataacc agaagcaaat caaaaaggtt gctttttcgg ccaacgaggt gggcgataag
    10441 atcaagctga taaaggccta taccgatcag gaggaaggct ttctggtggc ctccgaaatt
    10501 ctcaacacca tcagggataa gagttgtgcc tacagcgata ttgccattct gtaccgaact
    10561 aacgcccagt cgcgtatatt tgaggaaacc ctccgcaagc gaaacatacc ctacagggtt
    10621 tacggtagcc tatcattcta ccagcggaag gagataaagg atttgcttgc ctacttcagg
    10681 ctatccgtaa accctaatga cgatgaggcc tttgtacgaa ttgttaacta ccctgcaagg
    10741 ggtattggca gcaccaccct tcaacacctg caggaacttg caaacgtcca aaatataagc
    10801 ctctgggaaa cagctcaaca gctaaaacag cttaaccacc agttaaaacc tgctgctgta
    10861 aaaaagctga ccgattttgt tggtatggtt ggcggttttc aatcggctgt ttatacggcc
    10921 gatgcctatg aggcggccta ctccattgcc cgcaattccg gcatccttac cgatcttaag
    10981 gccgataaaa cacaggaggg gctttcccga ctgcaaaaca ttgaggagct gctcaacagc
    11041 atcagggagt ttattgaggc gcgcaggaat gaaggtgttg acgaaaccac tacgcttgtt
    11101 gactaccttg aaaatgttgc actgctcacc tccttggaca acgatgaccc caacgaccgg
    11161 aaccgtatta gcgttatgac catccattcg gctaaggggc ttgagtttga ttacgttttc
    11221 ctaacagggc tggaggagaa ccttttccct tcaaacatgt ccatgaacac gccggaggag
    11281 ctggaggaag aacgccggtt gttctatgtt gcgcttaccc gggcagccaa gggggtaacc
    11341 atttcatttg cccaaacccg gctcaaatgg ggaaacatta acatttccaa tcccagccgg
    11401 tttataagcg agattgatca gcgctacgtt gaatccccct ttgaacctga tggtggaggt
    11461 catctaaaag caaccagtga gctatcccga cagaccagca taaacaattt tgaaaaaaaa
    11521 tcaattggaa atcgggcagc tccctattca cagcccccgg gcaggacacc cgtatccgtt
    11581 ccggaaaacc taaaagagct tccacccgag gaaatttgcg aaggcgtgga ggtttaccat
    11641 ggaaagtttg gccagggtaa ggttttaagc gtcgaaggcg aaggcgctaa caaaaaggca
    11701 accgtgctat tcacggaggc aggccaaaaa aaccttctca ttaagtttgc caacctgaag
    11761 ggaatagctt agcttcagct taaccggaaa ggctgatgct gcaggtgcca actttttcaa
    11821 tcccttaagt cacgtgagga tgcttaaccc tttcaggcag tttgcatttt tacaaccgat
    11881 aacctaactt tgcagcataa aatgatgtac tgcattaggc ttaccgccag ctatttatta
    11941 catacatccc gaaatttttt atttttacct tttaaatatt tctaatggac tacaacaccc
    12001 agcgaaagaa actcgctctt ccggagtacg gaaggcacat acagcaaatg gttgactaca
    12061 ttgcatccgt tgaagatagg gacgaacgca acaggctggc aaaatccctt gtcgccataa
    12121 tggggaacct taacccccat ctcagggata ttaacgactt taaacataag ttatgggatc
    12181 acctgttcat catgtccgat ttcaagcttg atatagactc tccatacccc attcccagca
    12241 tggagcagta caacgaaaag ccccgtaggg ttccataccc gggtaaccct atcaagttta
    12301 agcattacgg gcgaataatt gaaatgatga ttgaaaaagc tgtagcaatg gaggaaggac
    12361 ccgagaagga tgccctcaag cagcttattg ccaaccagat gaaaaaggcc aacattacat
    12421 ggaacaagga gtcggtgagc gatgatgata tttttcgcga tatgcagacc ctaagcaacg
    12481 gcatgttggt aatggctccc ggatcaaagc tggtggataa caaggaattt aaaaacaaac
    12541 cccgcaccaa gtaccataaa aaacattagg gtggcatggc aacgttcgag gtaattggcg
    12601 gtaagcgtct aaaaggcgac ttatttcctc agggagctaa gaatgaggcg ctacaggtta
    12661 tttgcgccac cctgctaacc cccgagaaga ttaccataaa caacattcct gccatcaggg
    12721 atgtggtgaa gctcatcgag ctgcttcagg gtatgggggt cgaggttacc cgcttatccg
    12781 agtcgtcgta ttcatttata gccagaaata ttgacattga ttatcttacc actgacgatt
    12841 tcaaggttaa ggcagctgcc atccgtgggt caatcatggt ggttggtccc ttgcttgccc
    12901 gctatggcaa agcatatgca cccaaacccg gaggtgataa gattggaagg cgtaggctcg
    12961 acacccacct gcttggcttt cagaagctag gggtggagtt tgacttcgat gttaacgaca
    13021 actttttcac cctggagggg aaacacctga agggttccta catgctgctc gatgaggcat
    13081 cggttaccgg taccgccaat atcctgatgg ctgccaccat gactcccgga aaaaccacca
    13141 tttacaatgc agcttgcgaa ccttatgttc agcaactttg ccacatgttg gtttcgatgg
    13201 gcgccagaat ttctggaatt ggctccaacc tgctgaccat tgagggggta gatggtcttg
    13261 gtggatgcga gcacaccatt ctgcccgata tgatcgaaat tggctcgttt attgggctgg
    13321 ctgccatgac ctgcagcgat attacaattc gcaatgtctc attcggcaac ctgggaatca
    13381 ttcccgatgc ctttcgccga atggggataa acttcgaggt taccaacgac gatattcaca
    13441 tccctcccca cgagcactac gagattgaca cctttattga cgggtccatc ctcacgcttg
    13501 ccgatgcccc ttggccgggg cttacccccg atttgctcag cgtttttctt gttgtagcca
    13561 cccaggcaaa gggctcggtg ctgattcacc agaagatgtt cgagagccgc ctcttctttg
    13621 ttgataagct aatagacatg ggagcgcaga tcattctttg cgatccgcat agggcaaccg
    13681 ttattggcca cgacaggcag cttaggctga ggcctaccgt aatgacctcg cccgatattc
    13741 gggccggggt agccctgctt atcgcagcca tgtcggccga tggtaagagc attattcata
    13801 atatcgagca gattgaccgc ggctaccaaa acattgacgg caggcttaat gccattggcg
    13861 ctgaaattca cagaatcgat cgctaaagac cagggaagca atatttagtt ggtggttcag
    13921 caactgatgc atcgcagcca acactattcg accgattact tgttatgctt tttgatgagc
    13981 ttgttttcat gtgaatgttt tggtgaagct gtataagcgt aatgtttggc tggttaattt
    14041 caacaatgaa gttagcagtt ctttaatgca gcaggccttt tgcggggctt tttaatttgg
    14101 aattcacata gcagtcaata gtcgttaggc ttagcgaatt tacccggcta ctttctgaat
    14161 agttttcgca accgtaggcc aatgccaaca accggatgct ccggcaaaac ctttagctct
    14221 atctgagttt tggtcttagg tgaaatctgt gaaaggaaaa ggccagatac caccaccaga
    14281 ataccggcaa tcatcacagg tgtaagggct tcatcgagca ggaaataggc gaaaatggcc
    14341 gttagcaccg gaatggcgtt actgaaagtg tttgccctgg ttattcccaa gtgcttgatg
    14401 gaataggtga aaagcataaa tgcaaaagtt gatgcaaata ttccaagttt tataaccggg
    14461 accatagcct gccagctaat acctacagtt tggaagtggt tgaggtcaaa aataaggaat
    14521 aacggcatga agtaaagtgc cccaatggta ttctggtagg ttattatgct gtaaacgttg
    14581 tactttacgg ttagcttacc tacaatcaac gagtacccaa ctgttgaaaa aaccgccaga
    14641 aacatcaggg caaccccggt gaaattaaca tccacctgcc caaatccgtt atgaaagatt
    14701 accaggcata cgcccagcat ggaggcaata attcccatga ggttggtggt ggaaatcctg
    14761 cgatggaaca tgaaaaattc tccaactggg ctaagtaggg gaatggttga aacaattacg
    14821 gcagccatgg tggaggagat aagggtcata ccaaaactct cgccaataaa gtatagaagc
    14881 ggctcaaaga aggccagcag gaaaaacagc ttcaggtcgc ctttttttac ccgttctagc
    14941 ttcctcatag ccagtgaaaa aacgtaaagg aatgccgctg aaattaccag acggaagaac
    15001 actagcgtaa taggcctgta gtaaacatag gcatctttgt accagataaa agaaagcgcc
    15061 caaaaagcca ttgcaactgt gacggcaatg taaaccaaga actttttatt agacattccc
    15121 atacccttat gttatctatt gtttatccat tacgcaattt tctccacatt aacccattat
    15181 gtccggctag cgtagtccgg tttgtttttt tacttgatgt acctcatgga tattcccctg
    15241 ccattatttg agggtgaaag taaccaatgg tttatcgggt tgcaaatgta gcagatttgg
    15301 ctcacctaaa gctatcggat taatgcgatt ttggcattaa actagggaat aggtaaaaac
    15361 attgctgcgc tcgctttagc ggtagctatt ttatgggtat gtttaccagc tataacctaa
    15421 aacccatcac caggatatca tccacttgct tttgtgtgcc ctggaattct attaattgtt
    15481 tctgtagctc ttcgttttgc cttgataggc tgagcgtctg gagaccaagc agattttttt
    15541 tgaagttgac catcatcaac ttttggttca atgctccgcc aaactggtct gtgtaaccat
    15601 cggacataag gtagaatgtg tcgttggaga ggaggtcgaa cctgtgagtt gtaaaggggc
    15661 gttctcctgc cagatggtaa cctacgggca ttttgtcacc cttaatctca taaattgacc
    15721 atccgttggc tgtttcgggt tgaacaccag cgctaacctc aggcaattcg gagtttctgg
    15781 aaacaagtaa cggcatgtag gctccggcgt attctgcttt ccttcccttg gcatcaacaa
    15841 ccagcagccc catatccatt ccgtcatggg aatcctgcac ctgtcccgac tggttcagag
    15901 cggcgattac catctgccta agtttatcaa gcaaatcgcc agcagtgtta acttgttggt
    15961 aggctataat ctcctttagc agggtaatgc ccaaaatgct cataaaggcc cctggcaccc
    16021 catgaccggt gcagtcggct acagcaattg ctatacgtcc gtcgttcaaa cgggtaaccc
    16081 agaaaaagtc accgctgaca atgtttttgg gttggaagta aatgaagtag tcggtcagca
    16141 aggttgccag ggtatcggta cggggcataa ctgccgactg tatccggctg gcatacctga
    16201 tgctgtcggt aatttgcttt tgctggccgg ctatttggtc tcgttggcgt tcagccaggt
    16261 cgcgctggct ttcaatttcc tccttttgag cctcaatttc ctctttttgc ctgagtattt
    16321 cctggttctg ggccacgagt agggcgttaa tttttttctt gttacggtag aagtagaaga
    16381 ttaccccggc cagcgcaacg gcaaggagca gggcgatgga gatgaagagc gtcaacatac
    16441 gctgacgttc gagtttggct tgctgaatgg cctcacgctg gcgggatata agctcctgct
    16501 cccgctcccg cttgtcaaaa tcgtactgca tctccatgcg ggcaatctgg ctggcgttaa
    16561 tgctatcctt cactgctgag tagcgggtga agtagtctag cgaccttctg tagtcgcccc
    16621 tcaaccggta ggcctgggat aggctaagga atatgtcacg ctggtcggtg agcactcttt
    16681 gcctttgggc aatccgcatg gcttgttcca atgattccag acctgctgcc acattgccac
    16741 ttttcaggtt cacctctcca aggtagcggc ttagcttggc caggtcaatg ctgtcgttaa
    16801 cctgtctctt tagctccagc gaggtcaaca ggttgctttt tgctgaggtg taatcgccca
    16861 gctcaaagtt gataattccc agtaaaaggt ggtttgcagc ctgcccgtag atgttttgat
    16921 tttgctgatt tgtcgccagt acatcggtag ccatctcctt agcttttggg tacatgccca
    16981 agtggtagta aatattggcc agctctgttt tggccagagt aactccgttt atgttgctta
    17041 gctggcggta gtgggttagg gagctttcgg cgtactgcag ggctttttcg taaagatcga
    17101 ttcgggaaaa aagcctcgat atgctcagcg acgcccatgc tattccctcg tagtcctttg
    17161 cctgctcgta agtttccagc gccttcagca tgttctcaat ggcctccttg tagctacccg
    17221 tgatgctgta aactgttcca atgttcacca gcgttcgggc cagctggctc ttattgtcga
    17281 tactcctgtt aatattcaga gcctccgtgt atgcttgcag ggcgctgccg taatcgcttt
    17341 taagctggta aacattacca atcctgttca agttcatggc aacccagaac tttcgccctt
    17401 cttctgtggc tagctgcagg gatttcttaa gatgcgaaag agccagttca aaatcgtttc
    17461 gactgtagta gcaatttcca agagtgtgtt ctacccttac catgagttcc ttgttatcgc
    17521 tgccatcgat gatgttaagt gcctggatag ccaatcctat ggccgaatct ggattggtgg
    17581 ttcgtaagac ttcggcacgc tgaagcattt ctcctattct gaccgtatcg gattgagctt
    17641 gggagtagct caaggaaaat gtgaggagaa taagcaccag caaaatattt cgatggaatt
    17701 ctgtggagtt catagggcat tttttatttg ataaatatac cactttgacg gttttcatca
    17761 acatgtacag cgtattttat aagcaacttt tagccccggc tacaagctta ttacggtaac
    17821 cattttgttg ttacggggtg tttttctaat tttgcgccct ctatatggag gttaattttt
    17881 taaatgtcca accattaaat ggttgtaata caaaatatta tgaatgtagt taaggaaaat
    17941 accaacgaac aaaccatttc cattcgggca agggttgagc aggccgacta tgtcgaaaag
    18001 gttgaaaaga aacttaagga ctaccgtaaa aactcaaaca ttaaaggttt taggccaggc
    18061 atggcaccag cagcccttat acataggatg tactacaagt acgtgctggt tgacgaggtt
    18121 acgcatattg cctttgatgc cgtaatggat tatattgata aggagaaaat aaaaattctt
    18181 ggagagccat tgcccagcga ggggcagaat accataaact gggacaccga taaggaattt
    18241 gagctggcat gggaaattgg agtagccccc gagtttgaga ttaagcttgg gaaaaaggat
    18301 aaaattccat tctacaatat tgcccctgac gacaagatac gcaagcaata cattgacagc
    18361 tacctgatgc ggtttggaca ttttgccgaa gccgatgaga ccaacgaaag gggattaatc
    18421 gaggctactt taaccgagct taatagtgat gaaactcctc gcgaaggagg tttgtttgtt
    18481 gaaagagcca cgatctcggt tgagctggtt gccgatcctg atgaacgtgc taagctggta
    18541 ggagttaaag tgggtgatgt actcaccatt gatgttcaaa aagctttccc caatgaggcc
    18601 gacagggctt cattactttc tactcaaaag gagaatttgc ccaacattca gccagttttc
    18661 cagctcaccg taaacaaaac ttccaactac caaccggcgg agcttaacca ggagctgttc
    18721 gacagggttt ttggcgaggg ggtggttacc accgaggagg aattctacca gaaaattgac
    18781 gaggaaattg aatcgggact caagaatgaa ggcgaaaatc gctttgccgt tgagcttcgg
    18841 aatattcttc tcaataaaat cgacatgcag ctcccaaagg attttttaat caggtggctc
    18901 accaccatca acgagggtaa gtttacccgg gagcaggtag aaaacgagta cccgcttttt
    18961 gaaggggatt taaagtggca gctaatccgc aacaagattg ccagcgagca aaacttcacc
    19021 gttaccaacg aagagatgga aagttttgcc atggaatacg cccagcagca atttatggcc
    19081 tacggtatga gctaccttcc gaaagagtac attgagcgct atgccaagga tatattgaag
    19141 aaccaggatg aaatacgtaa aatgcaggag ggcattatca gccgcaaggt cattgcatgg
    19201 tttaaggaaa acctaccgct tgatgtaaag gagattacgc atgaggaatt tgataagctg
    19261 gagccggttt caggaaaata ggtggctggc gtaacagcag gttaaatagt tttgcctcat
    19321 ctcagctaat ttttgttgaa agttctaccg catttaacaa ttaggtgcta aattgcgtta
    19381 ctaaaccaaa tttaaaaagt aggcatcatg aatgaattcg aaaaatacgc aactaagcat
    19441 cggggtatca gcagcctatc gcttcagcgc tttggaacga taaccaacag ctacatttca
    19501 cccacaatta ttgaggagcg tcagctgaac gttgcaacca tggatgtttt ctccaggctt
    19561 atgatggata gaatcatatt ccttggagtg cctatagatg atgatgtggc caacattatc
    19621 caggcgcagc tgctattcct ggagtcaacc gatccttcga aggatattca gatatacatt
    19681 aactcgcccg gcggtagcgt ttatgccgga ctagggatat acgatacaat gcagtatatc
    19741 acctccgatg tggctacaat atgcacgggt atggctgcat caatggctgc ggtgttactc
    19801 acagctggcg aaaagggcaa gcgcacagca ttaccccatt cccgcgtcat gattcaccag
    19861 ccaatgggtg ggactgaggg gccagctacc gacatcgaga ttaccacccg tgaaattctt
    19921 aagcttcgcg acgagctcta tgaaattatt gccacccatt cgggaaaccc cattgaccgg
    19981 atacggaagg attccgaccg cgactactgg atgactgcca gggaagccat ggagtatggg
    20041 atgattgatg aggtccttac ccgatcaaaa aacttaaagg agactaaatg attcctttac
    20101 tgcaaaaaag cgccttaaag gcgctttttt acttcttgca gctgaatagc cacaaaagca
    20161 gctcgggctc tcggaatgcc tgatcccagc tattgtggcc agcatcggga tatacggtta
    20221 accttgcgtc gccacccaac tttcgtatgg agctaaccat atcaatggag tactgcggtg
    20281 gaactacatc atcctttgca ccatgaaaaa cccagaattt gaccttatgc gcatagcgtt
    20341 ttgccgattc cggattgctt cccccgcaaa tggggaaggc agcagcaaat acttttggca
    20401 tccttgccac cagctcaaag gttcccatac cccccatgga aaggcctcca acgtatatcc
    20461 gcctgctatc aacgtagctt tcccgtttga ggccattcac tagccggata agtaaatcca
    20521 tagctgctgt gggtttgctt tcgggtaaaa actcaataga acggtatcct tgctggttgg
    20581 ggttctcctt gatgtttgcc caaaaatcat cctcgggaca ctgtggaaaa acaacaattg
    20641 ccgggaaacg cgatcgaacc gaatcgttgg caagcagtga cgctccatga accagctgct
    20701 tctggttatc gttgccccgc tcacccgagc catgcagaaa gatcaccagt gggtacttct
    20761 gctctgggtt gaaattttta gggtacatta tccggtaggg taaggttttc cccttgtgag
    20821 taaaaacctt agcttcgtac agcgattcaa agccttgccc gacgaccttg tttattgaag
    20881 gcataattcc aaccatgcct atcaggcata gccggggtag ttttttccaa aactttttta
    20941 ctgattggtt taacatggta taagttgtta gctattagta ttttatgcat gtcaggtttt
    21001 atggctactc aagtggttga ttacagagtt ccctcatctg caaatgagta gcatttgcta
    21061 tcggtaatga taaggtgatc gagtagcgca atatcgaaaa aatctacagc ctgcttgagc
    21121 ttttccgtaa tggcaatatc cttttcgcta ggtttggggt ttcccgacgg gtggttatga
    21181 accacaataa tagcagaggc caacttttca acggctgccc ttatgattag ttttacatcg
    21241 gtaaccgtgc cggttacccc accctggcta accctaaccc tatccagcac cttgtttgcc
    21301 cggctcagga gcagcaccca gaactcctcg tggggcaggt cggccagtaa gggctgaaaa
    21361 atttccatca cattccggct agatgtaatc tgctcgcggt ttatggcctc cacagacttt
    21421 cgcctacggc ccaactccaa cgctgccaga agggttatgg ctttggcttc gcctactcct
    21481 ttaacctgtg taagctgatg gatggtaaac ttacccagct cgttgaggtt attctgggcc
    21541 atatttaata tccgttgggc aagctcaact gctgtttctt cagggtttcc ggaacgcagc
    21601 aggatggcaa tcagctccgt atcacttagg cttccaattc cctggtgaag cattttctcc
    21661 cggggccggt cctccacagc ccaatccttt atggttagcc ttttatactc ctgctccatt
    21721 ggtttgcctt tttcaactgg gtatgaatgg caagaaacag ctgctgtatc aagaattagt
    21781 tttagacatc aaacctccaa aaaaggctaa tttagctcct ttattgcata gttcatacag
    21841 gtgaatgcaa ctttttaaca gggaaaaagg gttgcatgcc atgctacatg caatggtgtg
    21901 gcctgatgtt ggggatgtgt aaaatttaaa acaagcgtag aaagtagatg ctatgggttg
    21961 tgcacatagg ttcactttgg caatgcttcc aggtctattc aatcgtaaac cagtaagttg
    22021 caacccctta tgggtgcatc atccatacga ccaagcacct cgaaacaacc atcggaatgg
    22081 agctttccca tatcctgggt ttgaatgaac gagcaggaat aaaggttggc caggtcaata
    22141 attttgataa ttcctgttct cccatcccct acaaggcaaa aggggtcatc ggtgctacct
    22201 ataaccaccc gcatccaggg tggacaccga aacatccctt taccggtaga atatgcctgt
    22261 gaaagcaatt ccgtcatgcc gtactcggag tgaatgacgt ttaccccaaa tgcgctgcaa
    22321 agcgttgagt gcagctcctc cctgacaacc tccttacgac gccccttcat acctccggtt
    22381 tccatgatga tggtattctg aaggttgaag gtaaaccttt cggccaggtc gagcagagca
    22441 aacgagaccc cgataaggat tgtcttttga ccggagcggt caagggcgct gagtttttcc
    22501 gttagggcat agagctggtt gaggtagaaa ccgctgtcag gattgccggt ttgcctgata
    22561 agctcatcaa ccatgaacac cagcgatgag cccgaacgtt caaggtatga gggcagaagt
    22621 gcaagaatgc agtagtccga gggagaaccg taaaatattg aaaacgtgcg ggtgaagctt
    22681 tcacggtaga gcgataaatc ggctacccga tggcggctaa cctcgttacc cgtagttcca
    22741 ctgctggtga aggttgcctc cggttggcat gcttcggcta taacatcctg cgttttgaag
    22801 agttgaatgg ggagaaacgg aatttcgtca gtggaagcaa ctgcatcggg gtttacgcca
    22861 agctgttgaa tgtagtcgtg gtaaaccctt acatgctggg actgaaacct gaaaacctcc
    22921 agggctattt tttcaaaatc accatccggt ttaaggctga atatgctgga gctgggaatc
    22981 atttgatttt gcctttacct gcttttacgg gttatgagta gctgaaaata caaagtcagg
    23041 gcttttttta aaaccctgac ggtatgctta aatggcgtga ttagagggtt gttatcctct
    23101 aattgcctta attccgggca gttcttttcc ttccatgtac tccagcatgg cgcccccacc
    23161 ggtggagacg aaggaaattt tatcggccag gtggttcttg ttgacagcag ctacggagtc
    23221 gcccccgcca accaggctaa acgctcccct ttgggttgct tcggcaatgg ccttggctat
    23281 ttctgatgtt ccggcagcaa atttgtccat ttcaaaaacg cccatggggc cgttccagag
    23341 gatggttttt gattcgagga taacctcacg gaacttagct atggttttgg ggccaatatc
    23401 cagacccatc cagccgtcgg gagtgctgtc gataggggta atctgggtgt tggcgtttgc
    23461 atcaaatgca tcggcattca ctgcgtcaac aggcaggtaa acctttactc ctttggcctt
    23521 tgctttttcg agcagttggt tagccagctc gagcttatcg gtttcgcaga tagacttacc
    23581 aacttttcct ccctgggcgc agatgaaggt gtaagtcata ccgccaccaa ttatcaggtt
    23641 atcaacccta tcgagcaggt tttcgattat caggattttg tcggacacct tggcgcctcc
    23701 catgatggct gtaaagggct tggtgggcga cttcagcacc ttgtccattg ccttgagttc
    23761 gctttcaata aggtagccaa acatgctgtt gccgggaaag tgggctgcaa taagcgccgt
    23821 tgaagcgtgt gcccggtggg cagttccaaa agcatcgtta acgtagcagt cggcatagct
    23881 ggccagctgc ttaacaaaat tcttctggct ctccttaacc cttgccttgg cttctttctt
    23941 ttcctcctcg ctggcggttt cgggaaggct gtaaggttta ccttcttctt cctcgtagaa
    24001 tctaagattt tcgagcagca acacctcgcc gggttttagg ccagctgcca tttttttggt
    24061 ttcctcgccc atgcagtcgg cggcaaattt tatcttctgc cccagatttt tttcaatagc
    24121 cggtaccacg tggcgcaacg agtacttatc ctgtggtgtg ccgtcgggcc ggccaaggtg
    24181 cgacatgagg atgacagctc ctccatcacc caaaattttt tttattgttg gaacggcacc
    24241 ccttatccgg gtgtcatcgg ttacctcaag ggttttcttg tcgaggggga cgttaaagtc
    24301 aacccttaca atggcttttt tccctttaaa gctatagctg tcaatggtta tcatggctta
    24361 acgggtattt gatttcaaag caaagttagc atagcgttgc aaaaaatagg ctatttcaag
    24421 cgaatatatt tacgcaatcg tttgaaaaat atcattttaa ggtatgtttt aataggaaag
    24481 gctacttctt acagcagtag tgagatttcc tgaatttcta agtaaatttt caggagcatg
    24541 aggtcgtaaa aataaataat caactcagca gctcgtcgtc tccggggtat gctggggctg
    24601 aatcgcaaac gtgaaatgat ttgaagtcaa gcccgggtag tctggcgtaa ataatttaga
    24661 aaattttcag ctgcatgtgg ggtaaagctg ccaatgggga tttgttttat ctgcattaaa
    24721 tgttaaagtc ttcctggtta atatgctact ttcctctgtg ttttgcttca tcaagctaat
    24781 gatgtgtcaa agcagctttt caaaaattat acttccaatc ttttcggaag gtggggatag
    24841 tgtccccttt ctttcaaatc cccttttctc caaaagtgct gccagattgc tgtcgtatag
    24901 tggcgagtag ccttccattg cagtaaagtt acccttttgg gctaaatcaa taccaaatga
    24961 gatcaacatg gaggccaacc ccttacgctg gaaagccggg aaaacggcca aacgactaat
    25021 tgtcatcact ttttggttgg tggcatgcgg ttttaaggtt acggttgcaa tggaaacatg
    25081 gttgtttacc aggacataca cgtagccctg ttcaatatca cttgatattt cctccatgtc
    25141 ggcatggctg ttgctccaga agttaacccc tttttcaacc agctggttgg agcattcccg
    25201 aatgatgtag agcacctcaa tcagctgctg tttttgtgct tgtctaattt ccatggctaa
    25261 aagcgttaga accaaaaacc tccttgccgg agacaaaggt tttcaggatg gttgcccttg
    25321 gaattgccgg cagctcatcg ttcatcaggt cggtgttgag aataacaaag tcggcagctt
    25381 ttcctggctc aaggctaccc ctagcatctt cgcagaaagc ggcttttgca gcccatatag
    25441 tcatggcgca gagggcctgt tttcgggtaa gggcgttctc gggttgaaat cctccgtcgg
    25501 gtttgccttc caggttttta cgggcaacgc cagcgtagaa accgtaaagc gggttgatac
    25561 tttcaattgg gaaatcgctt ccgttggcaa tccagccgtt ttgaagcagt agctcctgat
    25621 aggcataggc attcttaatt ctgctgccca gcctttcatc tgcccagtcc atatccgagg
    25681 tggcatgggt ggtttgaacc gaggggatta cggcaagtga accaaaacgt ggcaaatcgt
    25741 tcctgtcaac aacctgtgca tgttcaatcc gccacctcag gatatttcct gcaggcaagt
    25801 attttgagta gatgtcaagc atgagcttaa cagcagcatc gccaatgcag tgggtgcaca
    25861 cctggtatcc ggctttaaat gccatgccgc agagcgaatc gagtttttcc gttggttcaa
    25921 cctgaaggcc acggttacca cgatcgtcag agtatggttc aagaagcagg gcgcctcttg
    25981 agcccaacgc cccgtcggcg tatagcttta ttgaccggac ggtaagcttg ttggtccgat
    26041 acaccccttt tgaaataaac ctttcgtagt ttgccggggt tggattgagc atggcattca
    26101 cccagatttc cattttgccg gattgctgca gcgagtcaat aagcagcacc tcatcaacat
    26161 cgagccctgc atcctgaact ccggtcagcc caacggcaaa gcattttttc tgagcattct
    26221 gcaacgcagc ggctttttca tctatagttg gcttgggaat tatcctacgc accagctcca
    26281 tagcattgtc gagcagcaac cccgtaggtt ttcctttttg cagaaccact tcaccgcctt
    26341 caactcgtgt gctggcagta acgccggcaa ttttcaaagc agcattattt gcaatggccg
    26401 catggccgtc aatccgaaca atgtaaacag gtttatcggg gaatgcccgg ttaagcagct
    26461 cgttggtggg gaactcccta accttccact cgttctggtt ccaaccccta ccctcaaccc
    26521 agcttgttgg aaatctcttt tggtgctcta caagtctggc caccacttca tcccacgaac
    26581 ttgcctccga aagcatggcc ttgttcaggc ttaacccgta acccagaaaa tggcagtgcg
    26641 agtcaataaa gccagggtaa acatacattc ctccggcatc aactactttt ttggcagtgt
    26701 acttcgacat gatgtaaacg gaatcgccaa gctcctttac tattccatta accaccgcca
    26761 tgctgctatg ggtgctgaaa gttgaatcaa cggtgtaaat tttagcgttg gtaacaatta
    26821 agtcggcctt ttcacgtttg ctacatgcca tgagggtaac cgttagtaat aggtaaacat
    26881 accaattttt tgccatcttt gtatgtattg atttttaggc cattatttaa ccagccacag
    26941 ctatcaaatt tagtctttgt tttaataccc tcaacagctt ttgtaaattt gttacattgt
    27001 aatctctgaa tgttgaaata gacattagta atggcaaagt taccaacagc ctgcctttta
    27061 gcagtagcat ctattttcgc cttatgccat tcggcatgtg tgcgggacga tttttcggaa
    27121 agctcagcct tgaaactcag cttctcggcc gatacggttc tattcgacac cgtatttacc
    27181 actataggct cttcaacgca gtattttaag gtgtacaacc ggtcggggca cgaccttcat
    27241 atttcgtcta ttcgccttgc cggaggcagc acttcgccct accggattaa cgttgacggt
    27301 cgttcgggcg taaccttcag caatgtttcg ctacgctccc gcgacagcct tttcgttttt
    27361 gtggaggtaa cggttgaccc tacgtccctg aactcgccgt tgcttgttgc cgactccatc
    27421 atgttttcta ccaatggcaa tatccaggat gtgaagctgg tggcgtgggg tcaggatgtc
    27481 cacctactca ggtcagagat cattagcaac gatacggtgc tggctaccga caagccctac
    27541 ctgatctata gctacctttt ggtggatacc gctaccaccc tcaccattcc cgccggggta
    27601 cggatgtact tccataacca ggccatgctg gttgtggccg gaacactaaa agttgagggc
    27661 gagcaaggta gtcccgttgt aatggagggc gacaggcttg aggattttta ccgcgataag
    27721 gccggccagt ggggagggat ttggcttacg gccggaagcc ggtatagcgc tatcagctgg
    27781 gcagagatta gaaatgccat aacaggcatt acggttgata cctgttttac cagccatgcg
    27841 ccaactttaa ctcttagcca caccagaatt gaaaattgca gctatgtggg cctgctggca
    27901 cgtggggcaa aagtaaaggc cgataactgt ctggtgtcga acgctgcgca agtttgtgtg
    27961 gcccttacca tgggcggaga ataccgcttc taccactgca ctgttgccaa ctactggggc
    28021 gattacatct accgcaaagg gcctgcgctg ctgctaaaca actactacct ttaccgactt
    28081 gaggagaacg gcccgcttta cgttgaaccg cgtgatttgg tggaggcttc atttttcaac
    28141 agcattgtgt atgggtcgat gagcagtgaa tttcaggttg acaacatagt taacggtgca
    28201 ccggttgccg cacaaatgaa ctaccatttt gaagattgca tcctgaaggt tccccaaaac
    28261 ttcgacctca ccgataccga aaaatttacc cgggtattta cgctcgaccc caagttcaag
    28321 gattcaaatg cccatgtttt tctgctcgac acgcttagcc ccgccatggg aattgccaac
    28381 cctgctgttg ctaccgacta ccctattgac ctaaatgggt tcaaccggct taccgatggt
    28441 aaacccgacc ttggtgcata cgaaagacag gagtagtatg aggaagcttc tgataattct
    28501 gcccattttg cttgcttgtg ctgcctcaac aaaggttaaa gctcagaata tccctgccac
    28561 gcgccatcgc gtgatgttct acaacgtcga aaacctcttc gatccattcg atgactcgct
    28621 taccctcgac gaagagttta caccattcgg cgataggcac tggacatgga agaagatgga
    28681 gcagaagcta aacgggattt acaaggtgat tttggctgtt ggcgagtacg atccgcctgt
    28741 tttggtaggt ctttgcgagg tggagaatgg ctttgtgctg caccagctgg taagccaaac
    28801 gccgctttca aaattcgagt accgggtggt gcatcgcgag tcgcccgacc ggcggggaat
    28861 tgacgtagcg ctgctctatc gtcccgatag gttcaccctt caggagaagg agtttgtaac
    28921 ggtcaggttt ccggatgaac cctggcgcac aacccgggaa attctttacg tccgtgggct
    28981 tctaggaggc gacacgctac atgttttcgt taaccactgg ccatcgaagt acggcgggga
    29041 gctggagtca gaggggggtc gctatgctgc tgcctatgcc cttaaaaata aggttgactc
    29101 cataaaaatt ttctaccccg atgcccgtat cctcatcatg ggcgacatga acgatgagcc
    29161 cgaatcggat cccgtggcaa ggggatttgg cgcttgcctc acagccgaaa ctgcctgtcc
    29221 ttacgatttt gttaacctgt cggcaattct gaaacagcgt ggacagtaca gctacaagta
    29281 ccagggagtg tggggcataa tagaccagct aattgcgtca cgctccatgc tggatagccg
    29341 gcatgtgctg tacaccttta ccggcaatgc gggagttttc agcgcaccct ttttgcttga
    29401 ggacgacgag cggtatgccg gcaaacgacc atttcgaact tttattgggt ttaagtacca
    29461 cggtggctac agcgaccatt taccggttta cctcgatttg ttcagtaata agtagcgcgt
    29521 ttttcatgtg gagagcctcc gttgtccata acggccgagc gttgattttg tttgggggtt
    29581 acctccagcg ttgtcatcac ccggaataaa gttagtaaaa gtggggaacc ttgcaaacgg
    29641 aatcagccca a
//
LOCUS       DAOSHM010000031        27610 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_1353_length_27610_cov_22.876465, whole genome shotgun
            sequence.
ACCESSION   DAOSHM010000031 DAOSHM010000000
VERSION     DAOSHM010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 27610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27610
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1353_length_27610_cov_22.876465"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..909)
                     /locus_tag="PK839_06385"
     rRNA            complement(<1..909)
                     /locus_tag="PK839_06385"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            complement(1220..1945)
                     /locus_tag="PK839_06390"
     CDS             complement(1220..1945)
                     /locus_tag="PK839_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005646109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOG72402.1"
                     /translation="MKAMILAAGYGTRLLPLTQNKPKALIEVGGKPLLEHCIENLINF
                     GFNEIVINTHYFASQVISFLNSKTFDANIFISDETERLLDTGGGIFKARQFLDGNEPF
                     LVHNVDIISNIDLAELYNIHGQADILATLAVNQRDSSRVFLFNNDMQLAGWRNMVTGK
                     VIISNFSPQLTAYAFCGIHVISPRIFSAIKATGVFSIVDIYLNLCKDYRIQGVNLPNN
                     FILDVGNPDSIAIAESFLHSCNH"
     gene            complement(2203..3651)
                     /locus_tag="PK839_06395"
     CDS             complement(2203..3651)
                     /locus_tag="PK839_06395"
                     /inference="COORDINATES: protein motif:HMM:NF015620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HOG72403.1"
                     /translation="MDNLIMMMHALEEQHAELAKLFRNTFSVEPLCFEPIPVSGSLRK
                     YYRLQNGNTSAVGTFSPDVDENRTFFKLTDCFTRQSLNVPQVLAISPCMRYYLQSDLG
                     STSLFDIITSPGKFPQHNLNNFLSIAVTSLVDFQVKGFNEISSSGQLQLPLFDRTSMM
                     WDMYYFKYCFLKPSGISFNEQLLESDFEWFAKELLEQKHFLFQYRDFQSRNIMVHNNQ
                     LYFIDYQGGRIGPPLYDIASFLYQARAKLDNDKRFELLSLYLDRLNCLLSIDKEELKA
                     KFPAMAMFRIMQTLGAYGFRGFFEKKTHFIQSIPLAVDNFINLSRSAPGPHTAYITDL
                     LEQYRVLFQPSSCFSPSGQELTVYITSFSFKNGYPEEHPEHGGGFVFDCRCLPNPGRM
                     DRYKNLTGLDFEVIQFLDESDEAQRFIANATALVKDAVENYCQRRFSYLSVAFGCTGG
                     QHRSVYCASKLALAIENTKNVRVVVQHREFPA"
     gene            complement(3644..4321)
                     /locus_tag="PK839_06400"
     CDS             complement(3644..4321)
                     /locus_tag="PK839_06400"
                     /inference="COORDINATES: protein motif:HMM:NF016365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HOG72404.1"
                     /translation="MLGTIVNAVAILVGGVLGLVLKSRLPEKYTAVFFQVIGLFTLVL
                     GFSMSIKTEKPLLMVFSLILGGVSGIILNLQQRIDTWAIALKNRFKLKGDRFAEGMVV
                     AFLLFCMGSLTVLGALEEGTGGEPKLFYVKSLMDGFSALALASTLGVGVIFSILPLLV
                     YQGGLTWIAAATAKSLSNAMVNELSAVGGVLLIALGINILGIKKLEVVNLLPSLVFII
                     LLFYLFG"
     gene            4499..4957
                     /locus_tag="PK839_06405"
     CDS             4499..4957
                     /locus_tag="PK839_06405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72405.1"
                     /translation="MQLSLMRKDEIIDFIEFNITGDSENYNRCDDESLYLNTNVFNIF
                     ASCFERSNHLFDFISPTLYNSRNFIPLRNELLHNLETLLVIKNIAEFQNYMTGIFLGK
                     DLLKVLMDYGPNFERHWRGYMRKIIQVNRHLMRLIDQCIDEERVLWVIPY"
     gene            complement(4944..6041)
                     /locus_tag="PK839_06410"
     CDS             complement(4944..6041)
                     /locus_tag="PK839_06410"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase"
                     /protein_id="HOG72406.1"
                     /translation="MKLVADKDIPYLRGVLEPFFEVEYIPGAGISCDVVRDAEVLLVR
                     TRTRCNADLLQHSSVRFIGSATAGFDHIDSDYCSSNGILWRNAPGCNAGGVQQWVGAA
                     IVLWLETTQRLPQSLVLGVVGVGHTGSKVAELGKALGMKVLCCDPPRTRRENLPHFVN
                     LETILSSADIISFHVPLNTTGPDKTFHMLDSDSLQRCKPNSLLINASRGEVVDSHALT
                     AFLNANPFAHAAIDVWENEPNILNELLNRCMVGTPHIAGYSLEGKVAATQMVVDAVSQ
                     HYRLELHPWQPVPNPTLAKVKLNGISSLPEAISASYNIVGDDLRRISSDFEGYRNAYS
                     YRRDFSGFEVATSDPKLKQNLTRVGFTVSRG"
     gene            6205..6648
                     /locus_tag="PK839_06415"
     CDS             6205..6648
                     /locus_tag="PK839_06415"
                     /inference="COORDINATES: protein motif:HMM:TIGR00022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HOG72407.1"
                     /translation="MILSSLNEFGTYVNLHPLFSRIESALITMDFTHPGERIYIEGDN
                     LYATPSFSKAKAKEEALLEAHDRYIDVQVCLQGEEIMGWRNRSECLLPKEPYNEKDDI
                     TFFSDPPATFLKVPAYHFVIFYPSDCHAPLIGTGLIKKVVFKVKL"
     gene            complement(6797..7117)
                     /locus_tag="PK839_06420"
     CDS             complement(6797..7117)
                     /locus_tag="PK839_06420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72408.1"
                     /translation="MGKFSSKHVVEEIQGIRCTLVESGINKNRLDFLKSILEANGLKV
                     CFEAIPQAEGQEVLYKIGVTDLTFNPTIAIFGRRLKTPDGTVISPKYWLQETDSIKPY
                     YWLQ"
     gene            complement(7126..8550)
                     /locus_tag="PK839_06425"
     CDS             complement(7126..8550)
                     /locus_tag="PK839_06425"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HOG72409.1"
                     /translation="MLLQYLSLMRQEVSLIFIAVLVLICELTVNQRYKKVVIPITLVL
                     FGVHTILGFVPFDGGKLFGGMFATSHAQALMKSVLNVGVFIILLQSVAWLKKEVNFQK
                     SNEFLILLFSTLTGMYFMISSGDFLMFYIGLEIASIPMAMLAAFDTFRSKSSEAGIKF
                     LLNSALSSGILLFGISLFYAISGTIYFDDLPLYFSPVSPLFALGFIFFISGLAFKMSL
                     VPFHLWTADVYEGSPTIVTSYFSVISKGAAAFITSILLFKVFFNQLYLWKDIIYLLSV
                     LTMTVGNLFALRQTNIKRFLAFSSISQAGFILLGLVNVDSLGVTAIIYFILIYILSNL
                     SAFGVVMAIANVTGKEEIKDYNGLYKTNPLLSLTMMLAMFSLAGIPPTAGFFSKFFLF
                     TDAAKGGYYWLVFIAVLNTIISLYYYLLVVKAMFISPNEQPIERFRSDIYTRTALLIT
                     VVGIFAVGIYSPIFEYIRNIIDKF"
     gene            complement(8566..10065)
                     /locus_tag="PK839_06430"
     CDS             complement(8566..10065)
                     /locus_tag="PK839_06430"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HOG72410.1"
                     /translation="MNLSLFVIIPLLTVLLILFSKSIRQTRLIAAIGMSVQLVLSAIL
                     VVLYVSLRKHGNADQFVFVKDIEWFKSLNFNFTMGVDGISVALIGLTSIVIFAGVFAS
                     WKMEDLHREFFISLLVLAGGVFGFFITIDMFSMFVFYELAVIPMYILIGIWGTGKKEY
                     SAMKLTLMLMGGSALIIVGILGLYFHSGVSGGPLTMNLMNLSKVHFPVEVQMFLFPFL
                     FIGFGVLGALFPLHTWSPDGHASAPTAVSMLHAGVLMKLGSYGVFRIAIYLLPEAARQ
                     MGPFFMVLATIGIVYGAFGAIMQKDLKYINAYSSVSHCGLILFGLLMMNKTAMDGAIL
                     QMISHGIMTALFFALIGMIYGRTHTRLINEMGGLLKVMPFLGAAYVIAGLASLGLPGF
                     SGFVAEMLVFFGSFQHADLFHRILTILAASSIVITAVYILRVIGIFLLGPVKNEHHLL
                     LTDATWWERLSAGTLILFITIIGFYPYFLTNDLIGNSLEPILQKILMFN"
     gene            complement(10078..12006)
                     /gene="nuoL"
                     /locus_tag="PK839_06435"
     CDS             complement(10078..12006)
                     /gene="nuoL"
                     /locus_tag="PK839_06435"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018335971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HOG72411.1"
                     /translation="MQMSFQFTILIPLIPLISFLIIGFLYSKMSNKLAGIWGSVSIFS
                     SFLLSLFTAYRYFFEVGRVNGVYPKILGFDFEWLRFSDSLSAHLGTLIDPISVMMLVV
                     VTTVSLMVHIYSIGYMKGDRGFNRFFAFLSLFSFSMLGLVLSTNIFEIYIFWELVGVS
                     SYLLIGFYYEKNSAVAASKKAFIVTRFADLFFLIGILILSFYTKTFDFISLTDPSNIA
                     VMQTGSITFMGLSVTTWALLLIFIGGAGKSAMFPLHIWLPDAMEGPSPVSALIHAATM
                     VVAGVFLVARLFPIYALGSDQALQVIGYVGAFTSLFAAVIAMTQMDFKRVLAFSTISQ
                     IGYMMVGLGVSGYGNEAGLGYMASMFHLFTHAMFKALLFLCAGSVIHVVHTNFINEMG
                     GLRKHMKITHITFLIATLAIAGFPGFSGFFSKDEIITAAYHNNKIIFYTELLVAGITA
                     FYMFRLYFRVFWNQEASYEHKPHEVEKTMTIPLIILAFLSIAVGYIPFGNFISSDSMP
                     LESHINWGVASASIIVSFTGIIIAWVLYKKPSNLPERIVSSIKVLYTWAYHKFYIDEV
                     YLFVTKKIIFNLISRPIAWFDRHVVDGAMNGIAWTTSWVSESIKGLQSGQLQKYALAF
                     VSSTIILSALLIYILLNV"
     gene            complement(12012..12329)
                     /gene="nuoK"
                     /locus_tag="PK839_06440"
     CDS             complement(12012..12329)
                     /gene="nuoK"
                     /locus_tag="PK839_06440"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019151409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HOG72412.1"
                     /translation="MMGGLALQDFLVVSTSMFFIGIFGFLTRRNLITILMSVELILNS
                     VNINFVAFNYFLHPEAMHGIFFAIFVIAVAAAEAAVAIAIIINIYRRFKSINIENVSE
                     MKY"
     gene            complement(12331..12885)
                     /locus_tag="PK839_06445"
     CDS             complement(12331..12885)
                     /locus_tag="PK839_06445"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009599072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HOG72413.1"
                     /translation="MEIAASKVIFYIIAAAILFFALLSVTSKRILRAATYLFIVLIST
                     AALYFLLNYQFLGAVQIALYAGGIVVMIIFSILLTSHAGQKFEPVSRLKFFASLLAVL
                     VGWALSLWVILSHPFVASSKEAVEVNMDSIGKALLSYDRNGYVLVFEVISILLLAAMI
                     GAIVISKKQPGDGETNPDNSKTSK"
     gene            complement(12888..13364)
                     /locus_tag="PK839_06450"
     CDS             complement(12888..13364)
                     /locus_tag="PK839_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOG72414.1"
                     /translation="MGLKSYLSGLFKGFYSLLVGLRVTNSYFFHPSRIVTQQYPENRK
                     TLKMFERFKGEVILVHNENNEHRCTGCGICEMNCPNGSIEIISKMETGEDGKKRKAID
                     KHIYHLSMCTFCGLCVKSCPSKALAFGNEFEHAVFDRAKLDKVLNKEGSVLMKGVE"
     gene            complement(13376..14398)
                     /gene="nuoH"
                     /locus_tag="PK839_06455"
     CDS             complement(13376..14398)
                     /gene="nuoH"
                     /locus_tag="PK839_06455"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018335975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HOG72415.1"
                     /translation="MSDLWATVLEYAIIGIIYLVIYALIGLFLVYAERKVCAFIQVRL
                     GPNRVGPKGFFQTIADFIKLLLKENIKIKNADGVLFNLAPFIVIVASFLTFGTVPFAK
                     GLQGIDLNIGVFFIMAVSSMGVVGILLGGWSSNSKYSLIGAMRSGAQIVSYELSVGLS
                     LVTMVILTGTMQLSQIIELQSDGWFIFKGHIPALISLIIMMIAGTAEINRGPFDLAEA
                     ESELTAGYHTEYSGIKFAFFYLSEYMNMFVVSALVATVFFGGWMPLHIGSWQAFNHIM
                     DFIPPIIWFILKSFALMYVIMLFKWTFPRLRIDQLLKLEWQYLLPISLFNVLLMAFVV
                     YMKWYF"
     gene            complement(14511..16107)
                     /locus_tag="PK839_06460"
                     /pseudo
     CDS             complement(14511..16107)
                     /locus_tag="PK839_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133318.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
     gene            complement(16114..16701)
                     /locus_tag="PK839_06465"
     CDS             complement(16114..16701)
                     /locus_tag="PK839_06465"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007566210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HOG72416.1"
                     /translation="MAIKSMKESDFKDTDTLDQQMLEENGIFFTTVDSILNWGRSNSI
                     WPLTFATSCCGIEMMATAAARHDFARFGIEVYRASPRQADVIVVAGTIVNKMAPVLRR
                     LYDQMAEPKYVVAMGACAVSGGPFFYNSYHVLKGVDHLIPVDVYIPGCPPRPEAFLYG
                     IMQLQRKIKKETIKNKYARKNVANNYDEKNPLPEK"
     gene            complement(16692..17042)
                     /locus_tag="PK839_06470"
     CDS             complement(16692..17042)
                     /locus_tag="PK839_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005842860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HOG72417.1"
                     /translation="MSTQILIPFFITAVFLVAAGITLAYMLSPKSFNLQKQEPYECGI
                     PTVGVTWLQFNVGYYTFALLFLIFDVETVFLFPWAVVMKQIGLVGLVEILIFFFILVL
                     GLAYAWKKHALVWQ"
     gene            complement(17599..18588)
                     /locus_tag="PK839_06475"
     CDS             complement(17599..18588)
                     /locus_tag="PK839_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HOG72418.1"
                     /translation="MSTLVIVIVGLAFLYDFINGMNDAANSVATVVATRVLSPFVAVL
                     WAALFNFAAYFLFGLNVANTMGKGIIQPHVVEPWLVFSALLGASAWSYFCTHFGLPIS
                     VSHALIGGMVGPAWFAYGASSVVASGILNVVIFIFVSPLIGFVLGYLLMILIKHILKR
                     VKPANVDSWFRALQLLSSALFSLGHGSNDAQKTMGIIAVLLFSTGYLGSEFYVPEWVA
                     FSCYIAIALGTLTGGWKVIKTMGVNLTDLKPVHGFAAETAGAITLGMSAFMGIPVSTT
                     HTISGSIMGVGVTRRVSAVRWKVAQNIIGAWILTIPMTMIISGLLYLLIRVFV"
     gene            complement(18594..19241)
                     /locus_tag="PK839_06480"
     CDS             complement(18594..19241)
                     /locus_tag="PK839_06480"
                     /inference="COORDINATES: protein motif:HMM:NF013982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HOG72419.1"
                     /translation="MNVNSFFQFLAPKENKFYPHFNAASDNLVEVSLLLKKMMETDTL
                     DERLSFISKIKELERKGDDITHKMFDLLNGSFITPFDREDINALISSIDDVVDYINGS
                     AQQFQLYKPVSIPAEYIKMAEHIIEGSKTIQKAIYHIKELKNPVEIMDACIHVNEIEN
                     QADETYHRGIFQLFEHETNAIELIKKKDILQLLEKATDKLEDVSDVIKSILIKQS"
     gene            complement(19445..19804)
                     /locus_tag="PK839_06485"
     CDS             complement(19445..19804)
                     /locus_tag="PK839_06485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72420.1"
                     /translation="MEAKRFSERKLILFTVVLMIAAGLVRLVSYKVGVVLFYLSFLPF
                     IIHRLNFYHNHRGKLTDVDRRRRVTLALLLLMMLLNMVGWQNVEFILLIILAVDYMLI
                     NNRRSNGEKADDFGVKH"
     gene            20087..21850
                     /locus_tag="PK839_06490"
     CDS             20087..21850
                     /locus_tag="PK839_06490"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOG72421.1"
                     /translation="MKKYYTLLVILLLFLNATQSQPLTGTGSVANKQLLHHVSILASD
                     SMGGRFPGTTYDRISAKYIRDQLQSYGLSFQQKNGYQFFAIQTFNPNRATSTFLMVNG
                     KRLQYGNDYTALATSGNDSLCATAVFVGYGRTGGSADPDDYKNVSVKKKWAVMLVPSL
                     KVQDASSGELQQVDLSCINGAVGHEAAGIILIASEAENRFISMGSQQQALNLPVFILN
                     GKSSELILKSIGPADEVIGILERGGQNLSQDLPLTICGKAGKPYNEIETQNVVGVLRG
                     SDPNLADEYIVVGAHYDHLGMGGKEANSRMPDTVAVHNGADDNASGVAVMLEIAREMA
                     SQKKQLKRSVVFAAFGAEEMGLVGSQRFLDSAVVPIPNIKAMVNLDMVGRMVDGKLEI
                     GGTKTSMQAEAILNELNADSALAISLSPDGFGPSDHASFYSKNIPVFFFTTGIHQDYH
                     TPFDDVDKLNLTGMAQVTDYAYRLIYKLSTMDSSLTFSKSGSRMAPTNHGNKVKVKLG
                     IMPDVSGSTGNGLKVIAVSDDKPAMQAGIQSGDIITSLGGVPVTNIYDYTEQLGKLEP
                     GTTVEVKVLRNNQELTFTLKL"
     gene            21876..22700
                     /locus_tag="PK839_06495"
     CDS             21876..22700
                     /locus_tag="PK839_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72422.1"
                     /translation="MKKALLWIAAIIVTILAAIYQRDTGPTNPKRITYTIEGTTYKAK
                     LPRSGNSTSDCKVSLNLPLTSTATLYFRRYSTQDSFSPIPFIPEDEKTQSAYLPQMPP
                     AAKLEYYIVVNEGNNPMEIMRNSPVVIRYKGDVPAWALIPHILAMFLAMLYSSLAGLA
                     AAFKMESFRKFAIITLVLLLVGGFVLGPIIQFYAFGQAWTGFPLGFDLTDNKTLIALL
                     IWLAAVIANRKHSRPKIVIAAAIVTIVVFSIPHSTKGSELNYETGKVVSGVIMRVP"
     gene            complement(22803..24719)
                     /locus_tag="PK839_06500"
     CDS             complement(22803..24719)
                     /locus_tag="PK839_06500"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOG72423.1"
                     /translation="MKHAVLFLLGFPLPALAQPDSTSMKVNLDTVWVFSPQRVSSYSE
                     LSRMVGVVKRSELRQMKATSLSEVLERSASIDIRQRGTHGVQADINFRGGSFDQTLVM
                     LNDINISDPQTGHHNLNIPIVLDAVSRIEILQGPGARELGQGAFSGAINIITGPSAKN
                     EFSLSTFAGEYGLLKTSASATFGSAKIRTFTAASFERSNGYTDNTDFTGENFFFHTHF
                     YTGAGNFSLFSGYQDKGFGANSFYTPKYPNQFEATRTLLSGLKYIRAFGRTSISARGY
                     YRKNFDRFELFRENPPSWYMGHNYHTTDVYGLKANISMLYGFGKTEVGAEIRREEILS
                     SNLGEALSDSVRVKGRNAWYTKGGNRNLLNIFASQTFYLKRATLSGGLQLAYNSSFEY
                     IWCWGADISYSLNNHSRFFASVNRSYRVPTFTDLYYSSPTNVGNPNLKPEFATTIEGG
                     LKANRKAWSVQLAAFYRQGSSIIDWVKEPTDEAWHTINHTSLNTTGVEVFVQADLSEI
                     FRDAGSINFSYSFTSAEKNEDALLDSYYAMDYLRHNLRLALNQQFFKSLTLNLCYHFM
                     ARAGEFQDFATSQIRKYPGVHLADVRLTYSTTRKLDLFIDVSNIFNAHYMDVANVMQA
                     GRWVGAGMNLNFNL"
     gene            complement(25083..26105)
                     /gene="galE"
                     /locus_tag="PK839_06505"
     CDS             complement(25083..26105)
                     /gene="galE"
                     /locus_tag="PK839_06505"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007663244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HOG72424.1"
                     /translation="MDKILVTGGAGYIGSHTVVELVGAGYEVVLVDNFSNSSPEVLQG
                     IETITGVKPTFVEADCSDISAMRQLFSTHHGIQGIIHFAALKAVGESVDKPLQYYHNN
                     LLSTINMLAMMNEFKVKNLVFSSSCTVYGQPDELPVTEQSPMKPALSPYGNTKKICED
                     IIRDAAAANPDIRAIALRYFNPIGAHPSAYIGEQPRGKPDNLVPFITQTAAGVRDMLQ
                     IFGGDYHSPDGTAIRDYFHVVDLAKAHILAVERLMWGKNKSNFEVFNVGSGRGVTVLE
                     IVRAFMAVTGVNVPYEVVGRRAGDIEKVWADTSLAQKELGWKPESSLEDALLSAWRWE
                     KKLRGL"
     gene            26188..27183
                     /locus_tag="PK839_06510"
     CDS             26188..27183
                     /locus_tag="PK839_06510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72425.1"
                     /translation="MSDEQVNKTKATDEVDLIELFNRMGRGIKRGTVWLFKQVENLFI
                     LLIRKSLWIVSFAIIGLLVGYLIYKSSPRYYSSEMTAISNSVDNSHIVNSINLLNSLF
                     KEKNYSSASAYLDIPVGKAKQIKSIAAFYGIDLNRDKIVDYVDYANSFRFNPKDTMAK
                     RLTDYFFVSLEVYNEGAFTAAREGLKKYIYKNEFVVENNNERIAQNEEMIKVINIEIK
                     KIDSLQKIAYFETPLLQKASNGQMVFLNEKEVKLYHDQLLKLQRDKMRLERENKINKD
                     PITIVQDFTPLSKAENPYSKYGVKWGLIFAVIGFIASLLWQFRTKIVDYIKVKHY"
     gene            complement(27235..>27610)
                     /locus_tag="PK839_06515"
     CDS             complement(27235..>27610)
                     /locus_tag="PK839_06515"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOG72426.1"
                     /translation="IWQTIPSVVGKNWLFGLGIGDVRETLAKAYQEKNMDFALNKRFN
                     AHNQYLETYVGLGLIGLSCLLLILGSALWQSIRRRDMVFFLFMLIILINFMFESVLER
                     FFGVLFFVFFLSIFSMREVDSV"
BASE COUNT         8186 a         6444 c         6048 g         6932 t
ORIGIN      
        1 tgagtttcag ccttgcggcc gtactcccca ggtggatcac ttaatgcttt tgctttgccg
       61 ctgaacagtg tatcgccaac agctagtgat catcgtttac ggcgtggact accagggtat
      121 ctaatcctgt tcgatcccca cgctttcgtg cctcagcgtc aataccggcc cggcgagctg
      181 ccttcgctac cggcgttcct cgtgatatct atgcatttca ccgctacacc acgagttcca
      241 cccgccccga ccgtatccaa gagccccagt cccaacggca ttccagcggt tgagccgctg
      301 catttcaccg ccggcttaaa gccccgccta cgcacccttt aaacccagta attccggata
      361 acgcccggat cctccgtatt accgcggctg ctggcacgga gttagccgat cctttttccc
      421 ccggtaccgg caacgccccc cacagggggc ggtttcttcc cgggtaaaag cagtttacaa
      481 cccgtagggc ctccttcctg cacgcggcat ggctggttca ggcttccgcc cattgaccaa
      541 tattcctcac tgctgcctcc cgtaggagtc tggtccgtgt ctcagtacca gtgtggggga
      601 ccatcctctc agacccccta gccatcatcg ccctggtggg ccgttacccc accagccagc
      661 taatggcacg catgcccctc cggccccgcg tctccacttt gccggcaccc ccatgcggga
      721 ataccggttc atgcgggatt accccgcctt tcgacgggct acccccctgc gccggacagg
      781 ttgcatacgc gttacgcacc cgtgcgccgg tcgccatcag cgtatcgcta cgccatgctg
      841 cccctcgact tgcatgtgtt aggcctgccg ctagcgttca tcctgagcca ggatcaaact
      901 ctcccctgtc tcgtttcctt tttctctact ctgtcctctt cagaaccagg ccgctaatct
      961 tacttccttt tcctgtctct cggtgccatc gcgtcaacga actccgctac tcggccgggg
     1021 gctctaaccc cccccttttg cggctgcaaa tataccacct cccccaattc catccaaact
     1081 tttttacacc ttttttttaa gttttcaact acaattcttt attacaacat taccttgctg
     1141 attaacaatt acatcatgtc attcaaaata ttcagaaaaa ctaaaatttc tcaggtaaaa
     1201 agtgcagcag ttaatctggt taatggttgc atgaatgcag gaaactttcg gctatcgcaa
     1261 tggaatcggg atttcccaca tctaaaataa agttatttgg caagtttacc ccctgtattc
     1321 tgtaatcctt acaaagattt aggtaaatgt caactataga aaatactcct gttgccttta
     1381 tagcgctaaa tatccgggga gaaataacat ggattccgca aaacgcatag gcggtaagct
     1441 gggggctgaa atttgaaata atgaccttac ctgtaaccat attcctccaa ccggccagct
     1501 gcatatcatt gttgaagagg aacacccttg aggaatcgcg ttggttaacg gccagggttg
     1561 ccaggatgtc ggcttgccca tgaatgttgt aaagttcagc cagatcaata ttggaaatta
     1621 tatctacatt atgcaccaaa aaaggctcgt taccatcaag gaattggcgt gccttgaaaa
     1681 ttccaccacc ggtatcgagc aagcgctcgg tctcatctga aataaaaata ttggcatcga
     1741 atgtcttgct gttaaggaaa ctaataacct gtgaggcaaa gtaatgagta ttgataacaa
     1801 tttcgttgaa gccaaaattt atgaggtttt ctatacagtg ctccaggaga ggcttacctc
     1861 ctacctcaat cagcgcttta ggtttgtttt gggttagggg taataggcgg gttccgtaac
     1921 ctgcagcaag aatcattgct ttcatactag ttcaaaaatt agagaaaaac caacttaaaa
     1981 aacatcattt cgcaaaagct tagctaggac aaaactagaa aagcagtgaa aatcgacatt
     2041 taggttttgt taaagtaagc aattctattt aaaagttgac aagatagaaa tctaaaaagt
     2101 agaaatttat aagtaggcgt taaaaacaga attttttttg actgatttac aggtgagtat
     2161 gcgcttgcta tgcatgaaat aaaagacaaa atgtaacaag cattaagctg gaaactccct
     2221 atgctgaacg actaccctta cgttcttggt gttttcgatg gcaagggcta gcttactggc
     2281 gcagtacacc gaacgatgtt gcccaccggt gcagccaaaa gcaacagaca ggtaggagaa
     2341 acgtcgttgg caataattct caactgcatc ctttaccaag gcagtagcat tggcaataaa
     2401 tctctgagcc tcatcgctct catcaaggaa ttgaattact tcgaaatcaa gtcctgtaag
     2461 gtttttatat ctatccattc gaccagggtt aggcaaacac ctgcaatcga acacaaagcc
     2521 tcctccatgc tccgggtgct cctcaggata gccattttta aaagagaagc tggtaatata
     2581 tacagtaagc tcctgacccg atggtgaaaa gcaggatgag ggttgaaata aaactctata
     2641 ctgttctaaa aggtcggtaa tataagctgt atgaggccca ggagcggagc gagagaggtt
     2701 gataaagttg tcaacagcca ggggtatgct ttggataaaa tgtgttttct tctcaaaaaa
     2761 gcctctaaaa ccataagcac ctagagtttg catgatacga aacatggcca ttgctggaaa
     2821 tttagccttt aattcttcct tatctataga gagaaggcaa tttaacctgt ctaagtaaag
     2881 gcttagcagt tcgaaccgtt tatcattatc cagctttgct cttgcctggt ataaaaagga
     2941 ggctatatca taaagtggtg gcccgattct cccaccctga tagtctataa agtacagctg
     3001 gttgttgtgc accataatat tacgcgactg gaaatcacgg tactgaaata gaaagtgctt
     3061 ttgctctagc agctccttgg caaaccactc aaaatcggat tcgagtagct gttcgttgaa
     3121 tgaaattcca gagggcttga ggaagcagta tttaaagtag tacatatccc acatcatcga
     3181 ggtgcggtca aacaagggga gttgaagttg accggaagag ctaatctcat taaaaccttt
     3241 aacttgaaag tcaacaaggc tggtaaccgc aatggataga aaattgttaa ggttgtgctg
     3301 gggaaactta ccgggagaag taataatgtc gaaaagggat gtggagccca aatctgattg
     3361 caggtagtag cgcatacagg gtgaaatagc caatacttga ggtacattaa gggattgcct
     3421 tgtgaagcaa tcggtaagct tgaaaaaagt gcggttttcg tcaacatcag ggctgaaagt
     3481 tccaactgct gacgtgttgc cgttctgtaa gcggtagtat tttcgaaggg aacctgaaac
     3541 aggaattggt tcgaagcaga gaggttcaac gctaaaggtg tttctgaaga gttttgccag
     3601 ctcagcatgc tgttcttcta aagcgtgcat catcataatc aaattatcca aacaggtaaa
     3661 ataaaaggat aataaaaacc agggaaggta aaaggttgac tacctccagc tttttaattc
     3721 caagaatatt gattccaagt gcaataagca gtactccacc aactgcagaa agttcattaa
     3781 ccatggcatt ggatagagat ttggcagtgg ctgcagctat ccatgtaaga ccgccttggt
     3841 aaaccaacaa gggtaatatt gaaaagataa cgccaactcc cagggttgat gcaagcgcta
     3901 aggcagaaaa accatccatc aagcttttaa cgtagaaaag ttttggctct ccgccggttc
     3961 cctcttcgag tgcacccaga acagtcaaag agcccatgca gaaaagcaga aaagcaacaa
     4021 ccattccctc ggcaaaccta tcgcctttga gcttaaacct gtttttaaga gctattgccc
     4081 atgtatcaat tcgctgctgt aggtttagga ttatccccga aacacctcct agaattaagc
     4141 tgaaaaccat cagcagtggt ttctcggttt taatgctcat ggaaaatccc aaaacaagag
     4201 taaacaagcc tatcacctga aaaaaaactg cggtatattt ttcaggtagc cgcgacttca
     4261 gcaccaagcc cagcactcct cctacgagta tggccacggc attaactata gttccaagca
     4321 tattatacta atccttacaa atttcattgc taaaatacac attgcctgtt gatgtatggg
     4381 ttaatatcgt ccaaaaattg tttgaaattc aataaaatat taattttgac attaaatgag
     4441 cagttgtgaa aatcggaata ggcaacggtt ctactagtgg agacaaacta atgtcaaaat
     4501 gcagctaagc ttgatgcgca aggatgagat catcgatttc atcgaattca acattacagg
     4561 agactcggaa aactacaaca ggtgcgacga tgagtcgctt tacctgaata caaatgtttt
     4621 caacatattt gccagttgct tcgagcggag caaccacctt tttgatttca tatcgcccac
     4681 cctttacaac agccgcaatt tcataccttt acgaaacgag ctgctacata acctggaaac
     4741 cttgcttgtt ataaagaaca ttgccgagtt ccaaaactac atgaccggca tttttctggg
     4801 taaagactta ctgaaggtgc taatggacta cggtccaaac tttgagcggc actggagggg
     4861 ctacatgcga aagataatcc aggtgaaccg ccacctaatg cggctaatag accagtgcat
     4921 tgacgaggag cgggtacttt gggttatccc ctactaacag taaatcccac cctcgtcaga
     4981 ttttgcttta gcttaggatc gctagtggct acctcaaaac cgctgaagtc gcgccggtag
     5041 gagtatgcat tcctgtagcc ttcaaaatct gaactaattc tcctcaaatc gtcgccgact
     5101 atattatagc tggcgctaat ggcttcgggg agactcgaaa taccattaag cttaacctta
     5161 gccagggtgg gatttggtac gggctgccat ggatgcagtt ctagccgata atgttggcta
     5221 accgcatcaa ccaccatttg ggtggcagct accttaccct ccagggagta ccctgcaata
     5281 tggggtgtgc ccaccatgca acgattcagc aattcgttta gaatgttcgg ttcgttttcc
     5341 catacatcaa tagcagcatg ggcaaagggg ttggcattta aaaaggcagt taaagcatga
     5401 gaatccacca cctcgcccct tgaggcattt ataagaaggc tgtttggctt gcatctctgt
     5461 aaggaatcac tatcaagcat gtggaaggtt ttatcgggcc cggtagtatt caacggaaca
     5521 tggaatgaga tgatgtcagc gcttgacaaa atggtttcca ggttgacaaa atggggcaaa
     5581 ttctccctac gagttcgtgg cgggtcgcag catagaactt tcattcccag tgctttgcca
     5641 agttcagcca ccttgcttcc ggtatggcca actcccacca cgcccaaaac aagactttga
     5701 ggcaaccttt gtgtcgtttc caaccataaa acaatggctg cgccaaccca ctgctgcaca
     5761 ccaccggcat tgcatcccgg agcattgcgc caaagtatcc catttgaact gcagtagtca
     5821 gaatcaatat ggtcaaatcc ggctgttgcc gaaccgataa atcttacgct cgaatgttga
     5881 agcaaatctg cattgcaccg tgtacgggtt cgaacaagca acacctcggc atccctaaca
     5941 acatcgcagc taattccagc tccgggaata tactctactt caaaaaaggg ctccaggact
     6001 cctctcaggt aaggaatatc cttatcggct accagcttca tagctccaga ttttcattca
     6061 gcaggcaaag atagctaaat ggaatttccg gttgggccat caaaattttg ctcattttta
     6121 gctgtgataa tcacgattgt ttaccttagc taacaattac aggaactttt catctaaaca
     6181 aaacattgtt aattccgacc tgtcatgatt ctatcgagcc taaacgagtt tggcacatac
     6241 gttaacctac atccactttt ctccagaatt gaaagcgcct taataacgat ggactttaca
     6301 catcccggcg agaggatata cattgagggg gataatttgt atgcaacacc ctcgttcagc
     6361 aaggctaaag caaaggaaga agccttactt gaggctcacg acaggtacat agacgtgcag
     6421 gtttgcctac agggtgaaga gattatgggt tggcgaaacc gttcggaatg cctactaccc
     6481 aaagagccat acaacgaaaa ggatgatatt actttctttt ccgatccacc tgcaaccttc
     6541 ctgaaagtgc ctgcctacca ctttgtaatt ttttatccgt ccgattgcca cgccccactg
     6601 ataggcacag ggttaattaa aaaagtagtg ttcaaggtaa agctataacc tcaactggaa
     6661 aatccctgca actggctatc caaaacaggc caacagaatc taaggataac catgaatgat
     6721 gaaagattac cggataactt gcaaatccat gaggaattta ggggtttgca ggttaacggt
     6781 gctatggaaa gtctggttac tgcaaccagt agtaaggctt tatcgaatcc gtttcctgaa
     6841 gccagtactt tggagagatt accgtgccat cgggagtttt caaccgacgt ccaaaaatgg
     6901 cgatagtagg attaaatgtt aagtcggtta cgccaatctt gtagagtact tcctgaccct
     6961 cagcctgggg aatggcctcg aagcaaacct tcagcccatt tgcttccaga atacttttaa
     7021 gaaagtctaa cctattcttg ttgatacccg actcaaccag agtgcaacgg ataccctgaa
     7081 tttcctcaac aacatgcttt gagctaaatt tacccatagc cgtatttaaa atttatcaat
     7141 aatgtttcgt atgtattcga atatcgggct atagatgccg acagcaaaaa ttcccacaac
     7201 cgtgattagc agcgcagtcc gggtataaat atcgctcctg aagcgttcaa ttggctgttc
     7261 gttagggcta ataaacatgg ccttaaccac caggaggtag tagtagagcg aaatgatggt
     7321 atttagcacg gcaataaaaa caagccagta gtagccaccc tttgccgcat cagtaaaaag
     7381 gaaaaatttg ctgaaaaagc cagccgttgg cgggatgccg gcaagcgaga acatggcaag
     7441 catcatggtt agcgatagca acgggttggt tttgtataag ccgttgtagt cctttatttc
     7501 ctccttccct gtaacatttg caatagccat taccacgcca aatgcggata ggttggaaag
     7561 tatgtagatc aaaataaagt agataatagc tgtaactcca agtgaatcaa cgtttaccag
     7621 accaagaaga ataaagccgg cctgggagat tgatgagaat gccagaaatc ttttgatgtt
     7681 ggtttgccgg agggcaaaca ggttaccaac ggtcattgtg agcaccgaga gcaggtaaat
     7741 gatatccttc caaaggtaaa gctggttgaa aaaaacctta aagagcagta tggaggtgat
     7801 aaatgcagcc gcacccttag aaataacgga gaaatatgag gttacaatgg tgggagagcc
     7861 ctcgtaaaca tcggctgtcc aaagatggaa tgggactagc gacatcttaa aagccaagcc
     7921 agaaatgaag aatatgaatc ccagggcaaa gaggggtgaa acaggcgaaa agtacaacgg
     7981 caggtcatca aaataaatgg ttccactgat ggcataaaac aaggaaatcc cgaacagcaa
     8041 aatacccgac gacaaagccg agtttaggag aaactttatt cccgcctccg atgatttgct
     8101 cctgaaagta tcaaaagcag ccaacatggc cataggtatt gatgcaattt caagtccaat
     8161 gtagaacatc aggaaatcgc ctgatgaaat cataaagtac atcccggtaa gggtggaaaa
     8221 caacaggatt aggaactcgt tgcttttctg aaaatttact tccttcttca gccatgctac
     8281 tgattggagt agaataataa aaacgcctac gttaagtacg ctcttcatca gggcttgggc
     8341 atggctggtg gcaaacattc cgccgaacag cttaccccca tcgaaaggaa cgaatcccaa
     8401 aatggtgtgc acgccaaata atacgagggt gatggggata acaacttttt tatatcgttg
     8461 gtttacagtt aattcacata tcaatactaa cacagctata aaaatcagcg atacctcctg
     8521 acgcattagg ctaagatatt gtaatagcat aatttaggtt atcagttagt taaacatcaa
     8581 aattttttgt agaatgggtt ccaagctatt accaatgagg tcgttggtaa gaaaataggg
     8641 ataaaaacct attatagtga taaaaagaat caaggtacca gccgaaagcc gttcccacca
     8701 ggttgcatcg gtaagcagca ggtggtgttc gtttttgaca gggccaagca ggaaaattcc
     8761 gataaccctt aagatgtaaa cagccgtaat gacaatggag ctagcagcaa gaatggtcag
     8821 gattctatga aacaaatcgg catgctggaa cgagccaaag aatacgagca tttcggcaac
     8881 aaagccgcta aagccaggaa gtccaagcga ggcaagacct gcaattacat aagcggcgcc
     8941 aagaaaaggc atcaccttta aaagcccacc catctcgttg atcaacctcg tgtgggtgcg
     9001 accgtatatc atcccgataa gggcaaagaa gagggcggtc ataattccat gggagatcat
     9061 ctggaggata gcgccatcca tggccgtctt gttcatcatc agcaatccaa aaaggatgag
     9121 tccgcagtgg ctaacggacg agtaagcgtt aatgtacttt aagtcctttt gcatgatggc
     9181 cccaaaggct ccgtacacga ttccgattgt tgccagcacc ataaaaaatg gtcccatttg
     9241 cctggctgct tcgggtagga gataaattgc gatacgaaaa acgccgtaac ttccgagctt
     9301 cataagaact ccggcatgaa gcatagaaac agcggttggt gccgaggcat gtccatcggg
     9361 cgaccatgta tgcaggggaa ataatgcacc caaaacgcca aaaccaataa agaggaaagg
     9421 aaacaggaac atctgaacct caacgggaaa atgcactttg gacaggttca tcaggttcat
     9481 ggttagcggg ccgccgctta cacccgaatg gaagtataga cccaaaatac ccacaatgat
     9541 aagtgcagaa cctcccatga gcatcagggt gagcttcatg gccgagtact ccttcttgcc
     9601 ggttccccaa ataccaatta aaatgtacat gggtatcacc gccaactcgt aaaaaacaaa
     9661 catggagaac atgtcgatgg tgatgaagaa gccaaacacc ccacccgcaa gcacgagtag
     9721 cgagatgaaa aactcgcgat gcaggtcttc catcttccac gaggcaaaca caccggcaaa
     9781 aatgactatt gaggtaaggc cgattagggc aaccgaaatg ccgtcaaccc ccatggtgaa
     9841 gttgaaattt aagcttttaa accactcgat atcctttaca aacacaaatt gatcggcatt
     9901 cccatgtttt cgcaggctaa catacagcac aaccagtatt gcgctaagca ccagctgaac
     9961 agacattccg atagccgcta tcaaccgtgt ttgccttata ctcttagaaa aaagaataag
    10021 caaaacggtt agtagcggga tgattacaaa aagcgaaagg ttcatattct caccatgtta
    10081 gacgttcaac aaaatgtaga taagcaatgc gcttaaaata atggtgcttg aaacgaatgc
    10141 aagagcgtat ttttgaagtt gtcccgattg caaaccctta atactttcgg aaacccagct
    10201 ggtagtccat gctatgccat tcattgcccc gtcaaccaca tgcctgtcga accaggcaat
    10261 tggccttgaa atgaggttaa aaatgatttt tttggttaca aaaagatata cctcatcaat
    10321 gtaaaactta tggtaggccc aggtgtagag caccttgatg ctgctgacaa ttctttcggg
    10381 caggttggag ggtttcttgt aaagcaccca tgcaataata attccggtaa agcttacaat
    10441 aatggaagca gatgcaactc cccaatttat atgactttcc aggggcatac tgtcagaaga
    10501 aataaaatta ccaaagggta tgtagccaac agcaatggag agaaaggcta gaataatcaa
    10561 gggaatggtc atggttttct ccacttcatg tggcttatgc tcatagctag cttcctggtt
    10621 ccagaaaacc ctaaagtaca accggaacat atagaaggca gtaattcctg ccaccagcaa
    10681 ttccgtgtaa aaaataatct tgttgttatg gtatgcagca gtaatgattt catccttgct
    10741 gaagaatcct gagaagcccg ggaatccggc aattgctaga gttgcaatta aaaatgtaat
    10801 atgggttatc ttcatgtgct ttcgcagccc gcccatttca ttgataaagt tggtgtggac
    10861 aacatgaata accgatccgg cacaaaggaa caaaagcgct ttgaacatgg cgtgggtaaa
    10921 caaatggaac attgatgcca tgtagcccag gcctgcttcg tttccgtagc ccgaaactcc
    10981 caatcccacc atcatatagc caatttggga tatggtggag aaagctaaaa cgcgcttaaa
    11041 gtccatttgt gtcatggcaa taacggctgc gaacagggag gtgaaggcgc ccacgtagcc
    11101 aataacctga agtgcttgat cagaacctaa agcataaatc gggaaaagcc gggctaccag
    11161 gaatacaccc gcaacaacca tggttgccgc atgaattagg gccgacaccg gggatggccc
    11221 ctccatggca tcgggaagcc agatatgaag cggaaacatg gccgacttac ctgctcctcc
    11281 aatgaaaata agcagcaatg cccaagtggt tacgcttagc cccataaagg ttatacttcc
    11341 ggtttgcatt accgcaatgt tgctggggtc ggttagcgaa atgaaatcga acgtcttggt
    11401 gtaaaacgat aggattagaa ttcctattaa aaagaagagg tcagcaaatc gggttacgat
    11461 gaaggctttc tttgaagccg ctaccgcgga attcttttca tagtaaaatc caattagaag
    11521 gtaggacgaa acaccaacca gctcccagaa aatgtatatc tcaaaaatat tggttgatag
    11581 aactaacccc agcatggaaa agctgaatag cgagaggaaa gcaaaaaagc ggttaaatcc
    11641 cctgtcgccc ttcatgtagc cgatgctgta gatatgaacc atcagcgaaa cagtggtaac
    11701 caccaccagc atcatcaccg aaatggggtc aatcagtgtg cccaggtggg ccgacagcga
    11761 gtcgctaaat cttagccact caaaatcgaa ccctaaaatt ttagggtaca ccccgttgac
    11821 cctgccaacc tcaaaaaaat atctataggc cgtaaacaac gacaggagga acgatgaaaa
    11881 aatagaaact gaaccccata tcccagccag cttattggac attttactgt atagaaatcc
    11941 tataatcaaa aagctgataa gcggaataag cgggattagt atggtaaact ggaagctcat
    12001 ctgcatagtg gttagtattt catttcactt acattttcaa tattgatgga cttaaatcgg
    12061 cggtaaatgt tgataataat ggcaatggca actgcagcct cagctgctgc aacggcaata
    12121 acaaatatgg caaagaagat tccgtgcatg gcctcggggt gcagaaagta gttgaaggcc
    12181 acaaagttga tgtttaccga gttaagtata agctcaacgg acattaaaat ggtaatcagg
    12241 ttgcgtcggg taagaaagcc aaaaattcca ataaagaaca tggatgtgct gacaaccaga
    12301 aaatcttgta aagctaaacc tcccatcata tcatttgctt gttttagagt tatccggatt
    12361 ggtttcccca tcaccgggtt gtttttttga aataacaatc gccccaatca ttgcagccag
    12421 caggagaata gaaataacct caaataccaa aacatacccg tttctatcat agctaagcaa
    12481 ggctttcccg attgagtcca tgttcacttc aaccgcttcc tttgaacttg ccacaaaagg
    12541 gtggctcaga attacccaga gcgacaaggc ccatcctacc aaaacggcca acaacgacgc
    12601 aaaaaattta agacggctta ccggctcgaa tttctgaccg gcatggctgg tcaaaagaat
    12661 ggagaaaata atcatcacca caataccacc ggcatataaa gcaatctgca cggctcccag
    12721 aaactggtag ttcagcagaa agtacagagc tgctgtggat atcagtacga taaataggta
    12781 ggtggcagcc cgcagaatcc gcttgctggt aaccgagagc agcgcaaaaa aaagtatggc
    12841 cgcagcaatg atgtagaata ttacttttga ggcagctatt tccatggcta ttcaactcct
    12901 ttcattaaaa cagaaccttc cttattcaga accttgtcga gctttgccct gtcgaaaacc
    12961 gcatgttcaa attcgttgcc aaaggcaagc gcctttgatg gacagctctt aacgcacaag
    13021 ccgcaaaagg tgcacatgct caggtggtaa atgtgcttgt cgatcgcctt tcgctttttc
    13081 ccatcctcac ccgtttccat tttcgatatg atttcgatgg agccgttggg gcagttcatc
    13141 tcacatattc cgcaaccggt gcatctatgc tcattattct cgttgtgcac aagtataacc
    13201 tcacccttaa agcgctcgaa cattttaaga gtcttcctgt tctcggggta ctgctgggtt
    13261 acaattctgc tggggtgaaa aaagtaggag ttggtaaccc gcaggcctac taaaagcgaa
    13321 tagaatcctt tgaataatcc cgagaggtat gattttaatc ccataattta caagtttaaa
    13381 agtaccattt catgtacacc acaaacgcca tcagcagaac gttgaacagg ctaatcggga
    13441 gcaggtactg ccattcgagc ttaagcagct gatcaattct aagccttggg aatgtccact
    13501 taaacagcat aataacatac attagggcga atgatttcag tataaaccag atgatgggtg
    13561 gtataaagtc cattatatgg ttaaacgcct gccatgagcc gatgtgtagt ggcatccatc
    13621 ctccaaaaaa taccgtggca actagcgccg acacaacaaa catgttcatg tattcagaga
    13681 ggtaaaagaa ggcaaattta atgcccgagt actcggtgtg gtaaccggcg gtaagctccg
    13741 attcggcttc agccaggtcg aaaggccccc tgttgatttc ggcggtgccg gcaatcatca
    13801 tgattattaa ggatattagg gcgggtatgt ggcccttaaa gataaaccac ccatcgcttt
    13861 gcagctcaat aatctgtgat agctgcattg ttccggtcag gataaccatg gtaaccagcg
    13921 agagccccac cgatagctcg tagctaacaa tttgagcccc gcttcgcatg gcgccaatca
    13981 gcgagtactt actattgctt gaccatccac ccagcaaaat tcccaccaca cccatcgaag
    14041 aaaccgccat aataaaaaac accccaatgt tcaggtcaat tccctgcagc ccttttgcaa
    14101 agggaactgt gccaaatgtg aggaacgatg caacaataac aatgaaaggc gccaggttaa
    14161 aaagcacccc atcagcattt tttatcttga tattctcctt aagaaggagc ttgatgaagt
    14221 cggctatggt ttggaaaaat cccttagggc caaccctgtt gggacccagg cgaacctgaa
    14281 tgaaggcgca aaccttacgc tcggcgtaaa ccaaaaatag cccgataagc gcataaataa
    14341 ccaggtagat gatgccgata atggcatatt ccaacacagt agcccacaaa tcggacatgc
    14401 ttgctcgaaa taccgcatca atactctgta ttaacccagt aaaatcatat aaaacaccct
    14461 gcatagtatt agcgtttagt gctgaatggt taatgtttgc ccataagccc ttacctgtcg
    14521 atatcaggga ttaccaaatc caatgatccg ccaatggcaa ttaggtctgc aattttttga
    14581 tttcttgaaa tgaggtccat aaccgaaagg gttgaaaggc aaggtgaacg gaacttcacc
    14641 cggtagggtg ttttgttgcc atcgctgata atgtgcacaa cgagctcgcc acgggctgtt
    14701 tcaacctgct gaaagtactc gccttcggga agttttataa cagccttcat tttttccacg
    14761 tagctgccct cggggatatt atcaataagc tgctcgatga tcttcaacga ttccagcatt
    14821 tcatccattc taaccttgta gcgtgcaaag ctatcgccct cgttgtatag gatctcgtta
    14881 aactgcacct tgtcgtaagc gctgtacgga gctctcttcc tcacatcaca ggcaaatccc
    14941 gaaccacggc cagctgggcc tgtaacacca taggcaatag cctgctccct ggtcagaagc
    15001 cctataccca tagttcgatt ctgaaaaata acgttcccgg taaccagctc gtcgtactcc
    15061 ttgaaacttt tcctgaagta gggaataaac tccttaactc ttttctggaa gttgggatga
    15121 atatcgttca ttagacctcc cggacagttg tagctgtaaa gcaacctgcc gccggtggtt
    15181 tcctcaaaaa tatcgagaat tttttcccta tcgcgaaatg cgtaaaagta gggggttacc
    15241 gcccccatat ccatacacat ggacgaccac cagagcaggt gagaggcaat tcggttaagc
    15301 tcggcgataa tggttctgat gtacttaata cggtctgaaa cctctatatt taaagctttt
    15361 tcaacagcca ggcaaaccgc ctcgttgttg atgtgggccg aaaggtaatc catcctatcg
    15421 gtcagatgga tgatctgggg gtaagaaagg gattcgcaca tcttctcaat acccctgtga
    15481 atatacccgc aatgcggctg aacggattcc acaacctcgc ccttcagctt gaccacaaaa
    15541 cgcataacgc catgggttga aggatgctgg ggacccatgt tgatttccaa atcctgcccc
    15601 tcggtaccgg caaccgcaag atgtacaaat tcttctgcca ttgagctagt attttctttc
    15661 gatgatatta ctttcgtcaa cataatcctt tcggaggggg tgccctaccc agctttcgtc
    15721 caggaacatt cggcgcatat cgggatgatt attgaaggtg atacccatca ggtcgtaaat
    15781 ttcacgctca tggtactcgg ctgtttgcca gagagaggta acggaatcga cccggggatt
    15841 tgccttatca atattcctcg tttttaaaac catgtagtgg ttgtgggtgg tggaggtaag
    15901 caagtaggtg catccaaagc tactcacata atcaacgccg gtaatcagga ttaggtagtc
    15961 catttgggtt tgctcgtttt ccttaaggtt tctggccaaa tcaaagagct tgtcgaacgg
    16021 cacctccacg gtaaggaact gtgcttccga tattacggct tcaacaccca agcgttttac
    16081 tacttcctct agctgctggt tggtcatggt ttgctatttt tcgggtaaag gatttttctc
    16141 atcgtagttg ttggcaacat ttttcctggc gtacttattc tttatggtct ccttcttgat
    16201 tttccgctgg agctgcatga tgccgtacag aaaagcctcg ggcctgggtg ggcaaccggg
    16261 tatgtacacg tcaacaggaa taagatgatc gacccccttg agcacatggt atgagttgta
    16321 gaagaatggg cctcccgaaa cagcacatgc ccccatggcc acaacatact tgggttcggc
    16381 catctggtcg tagaggcgcc tgagcaccgg tgccatcttg ttgacaatgg ttccggctac
    16441 cacaataacg tcggcctgac gcggcgatgc cctgtaaacc tcaatgccaa agcgggcaaa
    16501 atcgtggcgt gctgccgccg tggccatcat ctcgattccg caacagctgg tggcaaaggt
    16561 gagcggccat attgaattgg accgacccca gttaagtatg ctgtcaacag tagtaaaaaa
    16621 gattccattc tcctcgagca tttgctgatc taaggtatcg gtatccttaa aatcagactc
    16681 cttcattgat tttattgcca taccaaagcg tgttttttcc aggcgtaggc taaacccaac
    16741 accaggataa aaaagaatat caggatttca accagaccta ccaaccctat ttgcttcatt
    16801 accactgccc agggaaacag gaaaactgtt tccacatcga atatcaggaa gagcagcgca
    16861 aaggtgtagt acccaacatt aaactggagc cacgtaaccc caaccgtggg aattccacac
    16921 tcgtagggct cttgtttttg caggttaaac gatttgggcg acaacatgta ggccagtgtg
    16981 attccggcag ccaccagaaa aacggctgta atgaaaaagg gaattaaaat ttgagtgctc
    17041 atgccattaa tgtatgaggt tgttcagcaa atttatccga gtagtaaata gtaaattgag
    17101 ttgctatcaa tcttaaatat ttcaggatgg agacccaaca catttttttt gcaaccacaa
    17161 aagtagattt tttgggtata tgatttaact aaaattcaca tttttttatt tttatatcta
    17221 tctattaaca taactggata acaaaaataa gtttttaacg atgtaagagc ttagcctaag
    17281 atttgctctc tattgcgaaa ggttttcaat actcccttct gcattggaga cttaatgtaa
    17341 aaccatcaag cttactcaat ggatatatgg tgggtagttt aggaagtaaa gtgaaaaaca
    17401 accattcttt aaatgttcta caagaattgc attagcctat ttataaccag gcttagctaa
    17461 atattttagc cataaactgc aaacagtttt cttccaaatc tggagagcat acggctagct
    17521 tagggtagta caataaaaca tgattggttt gaaaatgagc aaaccaaaat agctttcata
    17581 aaaactttca agagcctgtt acacgaaaac ccgaatgagc aggtagagca atccggaaat
    17641 gatcatcgtc attggaatgg taagtatcca agcacctatg atgttttggg ccaccttcca
    17701 ccggacagcc gagaccctgc gtgtaacccc aactcccatg attgagccgg aaattgtgtg
    17761 ggttgtgctc acgggaatgc ccataaaggc agacattcca agggttattg caccggcggt
    17821 ttctgcagca aagccatgaa ccggcttaag atcggttagg ttaaccccca tcgttttaat
    17881 caccttccaa ccgccagtta atgtgcccag cgcaatggcg atgtaacatg aaaaagccac
    17941 ccattcggga acataaaatt cggagccgag ataccccgtg ctgaaaagga ggactgcaat
    18001 aatacccatt gttttttgag catcgttgct accatgccca aggctgaaaa gggcagacga
    18061 taaaagttga agcgccctga accaggagtc cacatttgca ggtttaaccc ttttgaggat
    18121 atgcttaatc aggatcatca aaagatatcc aagaacaaaa cctataaggg gtgaaacaaa
    18181 aatgaagata accacattga gtattcccga agctacaacg ctactagccc cataggcaaa
    18241 ccaagccgga cctaccatcc ccccaattaa ggcgtgtgaa acgcttattg gtaggccaaa
    18301 atgggtacag aagtacgacc atgcagatgc accaaggaga gccgagaaaa cgagccatgg
    18361 ttcaacaaca tggggctgaa taataccttt gcccatggtg ttggcaacgt tgagcccaaa
    18421 caaaaagtaa gcggcaaagt taaacaatgc tgcccaaagc acagccacaa aaggcgataa
    18481 caccctggtg gcaacaacgg tagcaactga gtttgcggca tcattcatgc cgtttataaa
    18541 atcatacaaa aaggctaatc cgacgataac aattactagg gtgctcatgc tggctatgat
    18601 tgcttgatta aaatggactt gataacgtcg gaaacatcct ctagcttatc ggtagctttt
    18661 tcgagcagct gcaatatatc tttcttctta atcagctcaa tggcattggt ttcgtgttca
    18721 aaaagctgga atattccccg gtggtatgtt tcgtcggcct gattttctat ctcgttaaca
    18781 tgaatgcagg catccataat ctctaccggg tttttcagct ccttaatatg gtaaattgcc
    18841 ttttgtatgg ttttgctccc ttcaattata tgctcggcca tcttgatgta ttccgcagga
    18901 atcgatacag gtttgtacag ttggaattgc tgcgcagaac cgtttatgta gtctaccaca
    18961 tcgtcaatgg aagagataag ggcgttgata tcctcccggt caaatggtgt gataaacgat
    19021 ccgttaagta gatcgaacat tttatgcgta atatcgtccc ctttcctttc aagctcctta
    19081 attttactga taaacgacaa gcgttcatct aatgtatcgg tctccatcat tttctttaaa
    19141 agcagcgaaa cttcaaccag gttatccgac gctgcattaa aatgaggata gaatttgttt
    19201 tccttggggg caagaaattg gaaaaaactg ttcacattca tctttctgaa atatgttaag
    19261 tttatatgtt tgattttcaa ttataaagaa acacaaaaat aagctatgag aatctttcca
    19321 atgttaagta attgttaagt taactttaac tgcttggtat taagcaaata tgttgataat
    19381 caaatattac ataatttttt tggttaacca agtgctggaa gcaaataagg aaccctcaag
    19441 ttgtttaatg ctttacccca aaatcatcgg ccttttcacc attactccta cggttattaa
    19501 tcagcatata gtccactgcc aggatgataa gcaggatgaa ttctacgttt tgccatccaa
    19561 ccatgttaag aagcatcatc agcagcagaa gggctaacgt aacccgccgg cgccggtcaa
    19621 catcggtaag cttacccctg tggttatggt aaaagtttaa tctgtgaata ataaaaggca
    19681 ggaaggacag gtagaacaga acaacaccca ccttgtagct aaccagccta accagtccgg
    19741 ctgcaatcat cagcaccacg gtaaaaagga ttagcttacg ctccgaaaat cttttagcct
    19801 ccataaccta attagaagta atcagaattt gggattcaat gcagctgccc ggctgatgaa
    19861 aaaccaacag cttttgaacc cgcccccgca ccgcccgtca atatgtttag caagcaaaaa
    19921 tagatatttt ttggtcaacc agccggaatg gctaacggtg ttttggcaat cccgactagt
    19981 taccgtggca cttaaattgc aggccgtgaa cacttactga ttttatttca tatatcacta
    20041 tatttgacgg ttgaatttat aacggggtaa ctaccaaaca ataaacatga aaaaatatta
    20101 cacgctgctg gtaatcttac ttttattttt aaacgctacc caatcgcaac ctctaacagg
    20161 aaccggtagt gtagcaaata agcaactttt acatcatgta agtattttgg cttcagacag
    20221 catgggagga cggtttcctg gcacaaccta cgacaggatt tcggccaagt acatccgtga
    20281 ccagcttcag agctacggtc tctcctttca acaaaaaaac ggctaccaat tctttgccat
    20341 ccaaaccttt aacccaaata gggcaacaag cacatttctg atggtaaatg gcaaaaggct
    20401 tcaatacggc aacgactaca ctgcccttgc cacaagcgga aacgattcgc tttgtgctac
    20461 ggcagttttt gtaggctacg gccgcacggg cgggtctgcc gacccagatg actataaaaa
    20521 tgtttcggtg aagaaaaagt gggcagttat gcttgtcccc agcttgaagg tgcaggatgc
    20581 ctccagcggt gaacttcaac aggttgacct cagctgcatc aatggggctg tagggcacga
    20641 agcagcgggc ataatcctga tagccagcga agctgaaaat cgcttcattt ccatgggaag
    20701 tcagcagcaa gctctcaacc tgccggtttt tattctaaat gggaaatcat cagaattgat
    20761 acttaagagt attggcccag ctgacgaggt gatcggaata ctggaaaggg gtggacaaaa
    20821 tttgtcgcag gatttgcccc ttaccatttg cggaaaagca ggcaaaccct acaatgagat
    20881 agaaacccaa aacgttgtag gtgtcctgag aggaagcgac cccaacctgg ctgacgagta
    20941 cattgtggta ggtgcccact acgaccactt gggcatgggg ggcaaggagg ccaacagccg
    21001 gatgcccgat acggttgcgg tgcacaacgg agccgatgat aatgcctcgg gagtggctgt
    21061 catgctggaa atagcacgag aaatggcctc acaaaaaaaa cagcttaaac gcagcgtggt
    21121 ttttgctgcc tttggagccg aggagatggg gcttgtcggg tcgcagcgct tccttgactc
    21181 ggcggttgtt cccatcccga acatcaaggc catggtaaac ctcgacatgg taggcagaat
    21241 ggttgatggt aagctggaaa ttggcggcac taaaacctca atgcaagccg aagcaatcct
    21301 gaacgagctg aatgcggata gtgcgctggc catttcgcta tcgcccgatg gttttggccc
    21361 ctccgaccac gcttcgtttt actccaaaaa cattccggtt ttctttttca ccacaggcat
    21421 tcaccaggac taccacacgc ctttcgacga tgttgacaag ctgaacctta cagggatggc
    21481 gcaggtaacc gattacgcct accggctaat ctataagcta tccaccatgg acagctcgct
    21541 caccttcagc aagtcgggta gccggatggc acccacaaac cacggcaaca aggttaaggt
    21601 aaaactgggt attatgcccg atgtgagcgg ttccacagga aatgggctta aggttatagc
    21661 cgtttccgat gataaaccgg caatgcaggc tggcattcaa agcggtgata tcatcacctc
    21721 tttgggcgga gttcctgtta ctaacatcta cgactacacc gaacagctgg gcaagctgga
    21781 acccggcacc acggtagagg taaaagttct tagaaataac caggagctga cgttcacgct
    21841 taaactttag aaactacaaa ctaaaacata ccgaaatgaa gaaagccctt ctctggatag
    21901 cagccatcat tgtaaccatc ctggcagcta tttaccaaag ggacaccggc cccaccaacc
    21961 ccaaaagaat tacctacacc atcgagggaa ccacctacaa ggcaaaactt ccgcgcagtg
    22021 gcaacagcac atccgactgc aaggttagcc taaacctgcc gcttacctca accgcaaccc
    22081 tatacttcag gcgttactcc acacaggata gcttctcgcc catccctttc atccctgaag
    22141 atgaaaagac acaaagcgca tacctgcccc aaatgccgcc cgctgccaaa cttgaatact
    22201 atattgtggt aaacgaaggt aacaacccca tggaaattat gaggaatagc ccggtggtta
    22261 taaggtacaa gggtgatgta cctgcatggg cgcttatccc tcatatcctg gccatgttcc
    22321 tggctatgct ctactccagc ctggcagggt tggcagctgc cttcaaaatg gaatccttca
    22381 ggaaattcgc cattattacc ctggtgcttt tgctggttgg aggctttgtt ctgggaccaa
    22441 ttatccagtt ctacgccttt ggacaggcat ggacgggctt tccccttgga tttgacctga
    22501 ccgataacaa aactctcatc gccctgctaa tttggcttgc tgcggttata gccaaccgca
    22561 agcactcccg acccaagata gtaattgctg cagcaattgt tacgatagtg gttttctcaa
    22621 ttcctcatag cactaaaggg tcggagctca actatgaaac aggtaaggtg gttagcgggg
    22681 ttatcatgcg ggtaccttaa tttaagaaca cctttcaaaa ctcactggga atttttaaag
    22741 aaaaacatcg caggccaatt caaaataccg catgttcaac tttgattgca tttaaaacag
    22801 tactacaagt tgaaatttag gttcatgcct gcacctaccc aacgtccggc ctgcattacg
    22861 ttggcaacgt ccatatagtg ggcgttaaaa atattcgaaa catcaataaa cagatcaagc
    22921 tttctcgtgg tgctgtaggt aagccgaaca tcggccagat gaacaccggg gtacttcctg
    22981 atttgcgagg tggcgaaatc ctgaaactct cctgccctgg ccataaaatg gtagcagagg
    23041 tttagcgtaa ggctcttaaa aaattgctgg tttaaagcca acctcaggtt atgccgtagg
    23101 taatccattg catagtatga gtcgagcaga gcatcctcgt tcttttcagc gctggtaaag
    23161 gagtagctga aatttatgct accggcatcc ctaaaaattt cactcaaatc agcctgtacg
    23221 aaaacttcca cgccagttgt gttcaggcta gtatggttaa tggtatgcca cgcctcgtcg
    23281 gtgggctcct tcacccagtc aattatgctg cttccctgtc ggtagaatgc ggcaagctgc
    23341 accgaccacg ctttcctatt ggcctttagc ccgccctcaa tggttgtggc aaactcaggt
    23401 ttaaggtttg gattcccaac attggtagga ctgctgtagt atagatcggt aaaggttggt
    23461 accctgtatg agcggttgac cgaggcaaaa aacctggaat ggttattcag cgagtaggaa
    23521 atatcggcac cccagcacca aatgtattcg aaactactgt tgtaagccag ctgcagtcca
    23581 cccgacaggg tagccctttt taagtaaaag gtttgggagg caaaaatatt caataaattt
    23641 cgatttccac ccttggtgta ccaggcatta cggcctttca ccctcaccga atctgaaagt
    23701 gcctcaccca ggttgctgct aagaatctcc tccctcctga tttcggctcc aacttcggtt
    23761 ttgccaaatc catacagcat tgagatgttt gcctttaacc cgtaaacatc ggttgtgtgg
    23821 tagttatgcc ccatgtacca cgaaggagga ttctcacgaa aaagctcaaa cctgtcaaaa
    23881 ttcttacggt agtaacctct ggctgaaatt gaggttcgac caaaagcccg aatgtactta
    23941 agtccgctta gcagcgttct tgtagcctca aactggttgg ggtattttgg ggtgtagaag
    24001 ctattagccc caaaaccctt atcctggtaa cccgaaaata gggaaaagtt gccagcgccg
    24061 gtgtaaaaat gagtatggaa aaaaaagttc tccccggtga aatcggtatt atcggtgtag
    24121 ccattactcc tttcgaagga ggcagcagta aaggttcgta tttttgctga accaaatgtt
    24181 gcgctagcag aggttttcag caagccgtac tcaccggcaa aagttgaaag tgagaactcg
    24241 ttttttgccg aaggccctgt gattatattg atggctcctg aaaaagcgcc ctgtcccaac
    24301 tcccgggccc cggggccttg aagtatttca atacgactaa ccgcatcgag aacgatgggg
    24361 atattcaggt tatggtgacc ggtttgtgga tcgctgatgt ttatatcgtt gagcatgaca
    24421 agcgtctggt caaacgatcc tccccgaaag ttaatatcgg cctgcacacc atgagtaccc
    24481 cgctgacgaa tatcaataga tgcggagcgt tccagcactt ccgaaaggga agttgccttc
    24541 atctggcgca actcgctcct tttgacaacg ccaaccatcc gactaagctc cgagtaggac
    24601 gaaactcttt gcggggaaaa aacccataca gtgtccaaat ttaccttcat ggaagttgaa
    24661 tcgggctgtg ctaaagcagg caacgggaat cccaacagga aaagtacggc atgcttcatc
    24721 catacaaaat tttagtgcta ttaattgata atattctata aatcaacacc ctacacttgt
    24781 ttgtgctgaa atgaacccca aaggacgtgt cgtaggcaaa cgcccaaagt tttatagaaa
    24841 acccttacaa aaaatcaatg gtgtagtatg gcttaaaaac acgctgcaaa agtattacaa
    24901 gccggtaggt aaacatcacc ctatgactat gttaataaac atgatgagta aagccatcaa
    24961 cttgctgctg gtgttaatat ggaagaatgt attgggattg gcaaaaaaga attggacgct
    25021 tccgcacgtc caatctaaaa acttatgaac ctcagaaaag gccggaagag gctagtaggg
    25081 tgctataatc ctctcaactt tttctcccag cgccatgccg agagcagggc atcctcgagg
    25141 ctactttccg gtttccaacc cagctccttt tgagcaaggg aagtatcggc ccaaaccttt
    25201 tcgatatcac cggcacggcg gccaactacc tcgtagggaa cgtttacccc ggtaaccgcc
    25261 ataaatgccc taactatttc cagaacagta acaccacgtc cgctacccac gttgaaaacc
    25321 tcaaagttag acttgttttt cccccacatc aacctttcaa cagccagaat gtgggcttta
    25381 gccaggtcaa caacgtggaa gtagtcgcga atggctgttc catcagggga atggtagtcg
    25441 cctccaaata tttggagcat gtcccttacc cctgcagcgg tttgggtgat aaatggcacc
    25501 aggttgtcgg gttttcccct gggttgttcg ccaatgtagg cagaagggtg cgcaccaatg
    25561 gggttaaagt accttagtgc aatagctctt atatcgggat ttgccgcagc ggcgtcgcga
    25621 atgatatcct cgcaaatctt tttggtattg ccatagggcg aaagagctgg tttcatgggc
    25681 gattgctcgg ttacgggcag ctcgtcgggt tgaccataca ccgtgcagga gctggagaac
    25741 accaggtttt tcaccttgaa ttcgttcatc atggccagca tattaatggt cgaaagaagg
    25801 ttgttatggt agtattggag aggcttatcc acactctcgc ctacagcctt cagtgcagca
    25861 aaatgaataa tgccttgaat tccgtgatgg gttgagaaca gctgccgcat ggccgaaata
    25921 tcggagcaat cggcctcaac aaaagttggt ttaacgccgg taatcgtctc tattccctgt
    25981 agcacctccg gtgaggagtt ggaaaaatta tcaaccagca ccacttcata cccggcgcca
    26041 acaagctcaa caaccgtgtg cgaaccaata tacccggctc cacctgtaac aagaatcttg
    26101 tccattttta gatacttttg aacaaaagta atatatttgt tgccaaatca ggatttacag
    26161 aaaagagaat cactaaatca cgaatatatg agcgacgagc aagtaaataa aactaaagca
    26221 accgacgagg ttgacctaat tgagctattc aaccggatgg gaaggggaat taagaggggc
    26281 accgtgtggt tattcaaaca ggtcgaaaac ctatttattc tgctcattcg aaagtcgctg
    26341 tggatcgtat cctttgcaat tattggtttg cttgtaggct acttaatcta taaaagcagc
    26401 ccccgctact actcatcgga aatgacggcc atttcaaatt ctgttgataa cagccacatc
    26461 gttaactcca tcaacctgct caacagtctt tttaaggaaa aaaattattc atctgcatct
    26521 gcctacctcg acattccggt tggcaaggca aagcaaataa aatcaattgc agccttttat
    26581 ggtattgacc ttaatagaga taagatagtt gactacgttg attacgccaa ctcattccgt
    26641 tttaacccaa aggacacaat ggcaaaacgc ctgaccgatt acttttttgt aagcttggag
    26701 gtgtacaacg agggagcgtt cacggctgca cgtgagggat taaagaagta catctacaaa
    26761 aatgaatttg ttgttgagaa caacaacgaa aggattgccc agaacgagga gatgattaaa
    26821 gtgattaata tcgagataaa gaaaatagac agcttacaaa aaatagccta ctttgagaca
    26881 cccctgcttc aaaaagcatc gaacggccaa atggtctttc tcaacgaaaa ggaggtaaag
    26941 ctataccacg accagctcct taaactgcaa cgtgataaga tgaggttgga aagggagaat
    27001 aaaatcaaca aggatcccat taccatagtt caggatttta cccctctctc aaaagcggag
    27061 aacccttact ccaaatacgg ggtaaaatgg ggcttaatat ttgctgtaat aggatttata
    27121 gcctcgctgc tatggcagtt caggacaaaa attgttgact acatcaaggt aaaacattac
    27181 taaaaaaagg gcttcagctg aagccctttt ttaatctaca ataacgtcag aaatttaaac
    27241 agaatctacc tccctcatcg agaaaatgga gaggaagaat acaaaaaaca gcaccccaaa
    27301 aaatcgctcc agcactgact caaacatgaa gttaatcaga ataataagca taaacaggaa
    27361 aaataccata tcccttcttc taatcgattg ccaaagagcg ctacccagaa taagcagcaa
    27421 acagcttaac cctatcaatc caagacccac gtatgtctcc agatactggt tgtgggcgtt
    27481 gaagcgcttg ttaagtgcaa aatccatgtt tttctcctga taggccttgg ccagagtctc
    27541 ccttacatcg ccaataccca gcccgaatag ccagtttttt cccactacgg agggaattgt
    27601 ttgccagatt
//
LOCUS       DAOSHM010000032        27467 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_1366_length_27467_cov_20.156756, whole genome shotgun
            sequence.
ACCESSION   DAOSHM010000032 DAOSHM010000000
VERSION     DAOSHM010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 27467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27467
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1366_length_27467_cov_20.156756"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1260
                     /locus_tag="PK839_06520"
     CDS             <1..1260
                     /locus_tag="PK839_06520"
                     /inference="COORDINATES: protein motif:HMM:NF021021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M64 family metallopeptidase"
                     /protein_id="HOG72427.1"
                     /translation="SDFYTYFADRTMRIDYRHSGDDKTESFEMAKIASEGSWAGRTNH
                     LNDPWKLGLYFFEVKDAASGKTIYSQGFCSVFGEWQTTAEAKAGVKTFNETIRLPWPK
                     NEVNVVLQKRDSLNRLVPVWNLTVNPVEYEDLIPTRPSAFEPKVLLDNGDPKFKADIV
                     ILGDGYAENEDSLFWKDAQRFSDAFFSVEPFKSRIADFNVRAVLGKSTQSGIRLQRKD
                     FYPDNLLKTSYYAFGIERYVLASDDWIVKDYASIVPYDFIVILMNSDKYGGGGIFNLY
                     ITAAAHSRASEYVMVHEMGHHIAGLADEYYTSDVAYEPANTKVEPWEFNITALINPDS
                     LKWRDLVAPGTPIPTPWNKVEYEQGGGNKVLLSDPAAGKVGAYEGANYLSKGMFRPEV
                     NCIMIAGKEFCQVCKRGINRVLDLYCE"
     gene            1507..2535
                     /locus_tag="PK839_06525"
     CDS             1507..2535
                     /locus_tag="PK839_06525"
                     /inference="COORDINATES: protein motif:HMM:NF024929.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HOG72428.1"
                     /translation="MDSNVLVSLLVILLPLSFISERITNLVKLFLPTSNFLSIGNVRY
                     RENNPLLEKKRERRIFLISLLSGELVAFSLKADLFTILNSGKFGWDEPASGFSWIIGC
                     LFTGFFLSWGSKFWHDLLGIILEAKNMRRAIIDEKNLDVQLRAFGTQETSSPSEEVAA
                     VTIKPNQPQAAKPEFVQDKPTLQRIAKLHPLLREEAKEIYLQILAQGIAVRFTDTLRT
                     FAEQDELYAKGRTKPGKIVTQAKAGQSFHNYGLALDFCLLLADGREASWDRHTDINLN
                     LRYDWDEVVALFRHYGWEWGGDWVNFKDYPHFQKTFGLSTAQLLKLYNRKEVDGEGYV
                     VIKENEFS"
     gene            2643..3971
                     /locus_tag="PK839_06530"
     CDS             2643..3971
                     /locus_tag="PK839_06530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72429.1"
                     /translation="MGKRKVFLFIAAALLAIWVVGVVRDFDKLCKKTPNPEYQNFITQ
                     QKQNLQRTVEQLTTGEVIRVDGANLYRSDNCIGSNFEGAEVKLAGIQLYDSIYGSKSV
                     KPYYTYSCNGAILDPDHPLFGLPDIEVLIGPVTYYEPELSGVQALMNFGFSRDPKPHK
                     TFRKALYDSTGKKLQVFEMQLWLTYFKVAISVISNRDKPQITPDDESLNARIYPGKWY
                     CSSKPLISVNDLEQKEEYKNHQYGDITFVLEVNPNASPWYINTGSVHTARPDIAVGAV
                     ICKELVKRPEDENMMHLQTQRGMLTPLYFKPFERTVQPEKIDSIAGQVETEIQDYKNF
                     DIWNKPYYARLYSRNIGSRQKGFFGQKKFDEQVEYTFLLPLLVVGSWDVQLPWDVMPE
                     MQAPVPYYRGFNLKNLFPEWGIKSGGWISAGLLVAIVILLLLWFLPMLRR"
     gene            complement(3968..4747)
                     /locus_tag="PK839_06535"
     CDS             complement(3968..4747)
                     /locus_tag="PK839_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOG72430.1"
                     /translation="MLTRRNIQRIVSLRQKKHRNELGLFVAEGQRLVSELLKSHLDVA
                     EIYHTGNLEKMFTVDSKIATTLVSENEMNRISALSTPTPVLALVKMPLYNAEPINHAN
                     RLVLALDSIQDPGNMGTILRLADWFGIDTILCSNDTADIFSPKVVQASMGAIARIKVH
                     YCNLPTKLSAYSKNSPIFGTYLEGESIYDLPGVESGCIVMGNEGNGISPEVGSCITQK
                     IHIPDFAGGRARVESLNVAMATAIVLSEFRRKQVALINHET"
     gene            5049..7370
                     /locus_tag="PK839_06540"
     CDS             5049..7370
                     /locus_tag="PK839_06540"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HOG72431.1"
                     /translation="MLGVVFVSGCSITRNVPEDGYLLNKSNIKTDSKEISTRDMEDYL
                     KLKPNKKILGFRFHLRLYNMANPEKNKGVNKWLKNIGEEPVIMDTNLIAEGSRNLLNY
                     LQSKGFYNAKVEDSIYYHKKKANVIYSVYPNVPYRIKSLSYLVEDSAVLELVKKDTAN
                     CLIGVGKRFDTDMLQQERTRIEVFLKNNGYYSFSKDYITFTADTNFRSHSVNLILTIR
                     NPFTIDEKGNKVKSNFKKFKVKRVFIYPNFEPLEYLTYQQQATLDTVYYKDIQFIFSG
                     DAGVNLAVIYENILIRPGLTYSSEAVKKSQANLSSIRLFKFVNISFKEAKEAEFPISP
                     FDFLDEDSASSDSTEYAYLDCYIQLTQNTLQSYQVELVGTNTTGSLGAEANLSYQHKN
                     LFRGAEVFDFKLRGLVETAQQKINFDNTLELGGSMGLSIPKYIGPFSSRVQVSRSAPK
                     TQVTASYSYQHRPDYTRTIASLLFGYSWRSGSFITHNVNPAEVNAITISRISDDFKAY
                     IDTTSLKYSYISQIVTVSSYGFTFNNQNPQKPGSYTYLRYNFELSGNLLNLAFAAFNQ
                     PKNADGSYSIFNTNFSQFVRSDLNYTYNQYVDANNSFAYRIFLGVGYPYGNSKALPFE
                     KRYFTGGANGIRAWQARALGPGSYYQAKERYPNRTADIKLEANLEYRYKLFWKVEGAL
                     FIDAGNIWSMPGIDDRKGATFHFDKFYEQLALGSGLGFRMNLGFLILRTDFGYKIFDP
                     AINPGKPFRPWVPLQQKFMWRDITFNFGIGYPF"
     gene            7430..8488
                     /locus_tag="PK839_06545"
     CDS             7430..8488
                     /locus_tag="PK839_06545"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HOG72432.1"
                     /translation="MSYNRIVELLGDKSEFLLNHKCETVKKEQLHLPGPDFIERVVAL
                     SNRSPQVLRNLQGMFNHGRLAGTGYLSILPVDQGIEHSAGASFAPNPIYFDPENIVRL
                     AIEGGANAVASTFGVLASVSRKYAHRIPFIVKLNHNELLTYPNKYDQIMFGSVEEAWN
                     LGAVAVGATIYFGSEESTRQIIEVAQAFERAHELGMVTVLWCYLRNNAFKKDGVDYHV
                     AADLTGQANHLGVTLQADIIKQKAPENNGGFKAIGFGKTSDKVYSQLSSDHPIDLTRY
                     QVVNCYNGRAGLINSGGASSGSSDLAEAVFTAVVNKRAGGMGLISGRKAFQRPMDEGV
                     TILNAIQDVYLESKIDIA"
     gene            8731..9651
                     /locus_tag="PK839_06550"
     CDS             8731..9651
                     /locus_tag="PK839_06550"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOG72433.1"
                     /translation="MNKFKSKLKDYFSFTRSERNGTLVLSFLLVVFMLAPAAIRHIKF
                     SRNSPSSTMNTAIDSFFLSARFNPRNEVEKTTFSIAHEEVPRRAEPKQFPFDPNTISL
                     DSLVELGLSVKQAGVILKYREKGGHFDSATDLAKIYVLDSSTLARLKPLVRITAKSHE
                     QQLLNAESGSSSIFPVELNTADTTQLMRIKGIGRSYANRIVTFRNLVGGFYSVNQLAD
                     IYGITPELVASISPQVKVDSLAIRRINLNEVTYEELRSHPYLTDYQAKAIIYYRGKKG
                     SIGNPHELVQNKLVPADKFQKIYPYLITVR"
     gene            9790..9981
                     /gene="rpsU"
                     /locus_tag="PK839_06555"
     CDS             9790..9981
                     /gene="rpsU"
                     /locus_tag="PK839_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006045671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HOG72434.1"
                     /translation="MIIVPIKEGENIERALKKFKRKFEKTGIMRELRSRQAYTKPSVR
                     RREEIKRAIYIQQLQQKEE"
     gene            10065..10949
                     /locus_tag="PK839_06560"
     CDS             10065..10949
                     /locus_tag="PK839_06560"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOG72435.1"
                     /translation="MNMLASFLKHIEVERGYSSHTVRSYGDDIRQFLNFYQPEASEVD
                     FSQITHRHIRAWVVFLMSEGISERSINRKLSSLRTFFRYLKNQGAISSNPIVKVVPPK
                     AGKRLPEFVPESDMEKLEADNLFPASFEGIRDSLIVLLLYYSGIRLSELVGLSLSSID
                     TNSQTMKVLGKGNKERVVPLHPDIKPLIQRYIAARQEVVADGCSAFFITCKGKPIYPK
                     LAYRVVNRYLSMVTPLQKRSPHVLRHTFATHLLNQGAELNAIKELLGHSNLLATQVYT
                     HNTFEKLKRNYNQAHPRA"
     gene            10971..11270
                     /gene="raiA"
                     /locus_tag="PK839_06565"
     CDS             10971..11270
                     /gene="raiA"
                     /locus_tag="PK839_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HOG72436.1"
                     /translation="MNIKIQSIKFDADKKLTDFIHKKVSKLERLFDNIVEVEVFLRLE
                     SSQVQENKIAEIRIKVPGSDLFAEKRCKTFEEAVDECISALKIQVKKHKEKLRGE"
     gene            11464..11537
                     /locus_tag="PK839_06570"
     tRNA            11464..11537
                     /locus_tag="PK839_06570"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11498..11500,aa:Thr,seq:tgt)
     gene            11551..11633
                     /locus_tag="PK839_06575"
     tRNA            11551..11633
                     /locus_tag="PK839_06575"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11585..11587,aa:Tyr,seq:gta)
     gene            11711..11783
                     /locus_tag="PK839_06580"
     tRNA            11711..11783
                     /locus_tag="PK839_06580"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11744..11746,aa:Gly,seq:tcc)
     gene            11795..11866
                     /locus_tag="PK839_06585"
     tRNA            11795..11866
                     /locus_tag="PK839_06585"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11827..11829,aa:Thr,seq:ggt)
     gene            11928..13115
                     /gene="tuf"
                     /locus_tag="PK839_06590"
     CDS             11928..13115
                     /gene="tuf"
                     /locus_tag="PK839_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HOG72437.1"
                     /translation="MAKEKFDRSKPHVNVGTIGHVDHGKTTLTAAITKILAEKGLSEF
                     RSFDSIDNAPEEKERGITINTSHVEYQTEKRHYAHVDCPGHADYVKNMVTGAAQMDGA
                     ILVVAATDGPMPQTREHILLARQVNVPRIVVFLNKCDAVDDPEMLDLVEMEVRELLTF
                     YQFDGENTPVIRGSALGALNGEPKWVEKVMELMAAVDEYIPIPPRENEKPFLMPVEDV
                     FSITGRGTVATGRIETGVIHTSDEVEIIGLSHEKMKSVVTGVEMFRKILDQGEAGDNV
                     GLLLRGIDKKEIKRGMVVAKPGSITPHTKFKAEIYVLKKEEGGRHTPFHNHYRPQFYL
                     RTLDVTGEIVLPEGVEMVMPGDNVTITVELIYPVAINKNLRFAIREGGRTVGAGQVID
                     IIE"
     gene            13162..13234
                     /locus_tag="PK839_06595"
     tRNA            13162..13234
                     /locus_tag="PK839_06595"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13195..13197,aa:Trp,seq:cca)
     gene            13251..13442
                     /gene="secE"
                     /locus_tag="PK839_06600"
     CDS             13251..13442
                     /gene="secE"
                     /locus_tag="PK839_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HOG72438.1"
                     /translation="MKITKYFHEVYTEMVHKVSWPTMKELQSSALIVIVASLLIALVV
                     FVMDISFENLMKVIYRMLN"
     gene            13466..14014
                     /gene="nusG"
                     /locus_tag="PK839_06605"
     CDS             13466..14014
                     /gene="nusG"
                     /locus_tag="PK839_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HOG72439.1"
                     /translation="MEENVKKWYVLRAISGKEKKVKEYLENEITRLKLQDFISQVLIP
                     TEKVYQIHNGKKISKERIFYPGYVLVEAALVGEVPHILRNAPNVLGFLGDPKSGDPIP
                     LRTSEVNRILGKVDELVDSEEELNVPFFVGETVKVIDGPFNSFSGVIEEVNDEKKKLK
                     VMVKIFGRKTPLELSFMQVEKE"
     gene            14038..14478
                     /gene="rplK"
                     /locus_tag="PK839_06610"
     CDS             14038..14478
                     /gene="rplK"
                     /locus_tag="PK839_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006045676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HOG72440.1"
                     /translation="MAKEIAGFIKLQVKGGAANPSPPIGPALGSKGVNIMEFCKQFNA
                     RTQAQAGKVLPVIITVYNDKSFDFVVKTPPVPVQLLEITKQQKGSAEPNRKKVASVTW
                     EQVRQVAQDKMPDLNCFTLESAMKMVAGTARSMGIAVTGEFPAK"
     gene            14500..15201
                     /gene="rplA"
                     /locus_tag="PK839_06615"
     CDS             14500..15201
                     /gene="rplA"
                     /locus_tag="PK839_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HOG72441.1"
                     /translation="MTKLTKNRKLALSKVEPNRQYKLSEAAALLKDITYTKFDASVDI
                     DVRLNVDPRKANQMVRGVVTLPHGTGKQVRVLVLCTPDKEEAARQAGAEFVGLDEYIE
                     KIKGGWTDVDVIITMPTVMGKIGALGRILGPRGLMPNPKSGTVTMDVDKAVKEVKQGK
                     IDFKVDKFGIIHASIGKVSFEPEKIVDNAIEFLNTIIKLKPTTAKGTYLKSIYLSTTM
                     SPGLAIDTKSIAAAN"
     gene            15229..15753
                     /gene="rplJ"
                     /locus_tag="PK839_06620"
     CDS             15229..15753
                     /gene="rplJ"
                     /locus_tag="PK839_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HOG72442.1"
                     /translation="MTREEKKNLIDALTETINRYPHFYLTDTSALNAEDTSKLRRSCF
                     QKEVKMVVVKNTLFKKALEQSGKFDFSPLFTELKGTTSVMFTESGNVPAKLIKEFRTE
                     HPKPLLKAAFVEESIYVGDEQLNMLASLKSKNELIADVIALLQSPAKNVIASLQSGGN
                     ILSGVVKTLSEKEK"
     gene            15788..16165
                     /gene="rplL"
                     /locus_tag="PK839_06625"
     CDS             15788..16165
                     /gene="rplL"
                     /locus_tag="PK839_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HOG72443.1"
                     /translation="MADLKKFAEDLVNLSVKEVNELAKILKDEYGIEPAAAAVAVAAA
                     PAAGAGAAAAEKTQFDVILKAAGGAKLQVVKLVKDLTGLGLKEAKDLVDAAPKPVKEG
                     VSKEEAESLKAQLEDAGAEVELK"
     gene            16260..20084
                     /gene="rpoB"
                     /locus_tag="PK839_06630"
     CDS             16260..20084
                     /gene="rpoB"
                     /locus_tag="PK839_06630"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007212060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HOG72444.1"
                     /translation="MSNNNNNNRISFSRTKSQYEYPDFLEVQLKSFTEFFQLGTSPEE
                     RRHEGLYKVFLENFPITDTRNNFVLEFLDYFVDPPRYSIEECLDRGLTFCVPLKAKLK
                     LYCTDPEHEDFDTVVQDVYLGTIPYMTPRGTFVINGAERVVVSQLHRSPGVFFGQSIH
                     PNGTKLYSARIIPFKGSWIEFATDINNVMYAYIDRKKKLPVTTLLRAIGYESDKDILE
                     IFNLAEEVKVNKVNLKKIVGQRLAARVLKSWVEDFVDEDTGEVVSIERNEVILDRETV
                     VENEHIDTIIESGAKSILVHKENQTFSDYAIIYNTLQKDPCNSEKEAVVYIYRQLRNS
                     EPPDEATARDVIDKLFFSDKRYDLGEVGRYRINKKLNLTTSPDVKVLTREDIIEIIKY
                     LIELVNSKAEVDDIDHLSNRRVRTVGEQLAAQFSVGLARMARTIRERMNVRDNEVFTP
                     IDLINSKTLSSVINSFFGTNQLSQFMDQTNPLAEMTHKRRLSALGPGGLSRERAGFEV
                     RDVHYTHYGRLCPIETPEGPNIGLISSLCVYARINDLGFIETPYRKVDGGTVGMNDDD
                     VVYLTAEEEEAKIIAQANAPIGDDGSFVESKVKARYEGDFPLAESESIDLMDVAPNQI
                     ASIAASLIPFLENDDANRALMGSNMMRQAVPLLKPETPIVGTGLEGKVVSDSRVQVVA
                     EQDGVVEYVDANEIVVRYNRSEDEVFVSFDPELKRYSLPKFKKTNQNTSITLTPIVTK
                     GQIIKKGDILTEGYATRNGELALGRNLKVAFMPWKGYNFEDAIVINERLIRDDMFTSL
                     HIDEYILEVRDTKRGMEELTADIPNVSEEATKNLDENGMIRIGANVVPGDILIGKITP
                     KGESDPTPEEKLLRAIFGDKAGDVKDASLKASPSLYGVVVDKMLFSRAIKDEKDQKKS
                     KSTEKSKLEKIDNEFNRQVAELQTKLVDKLFVLVNGKTSQGVVDNFNQELIPRGVKFT
                     HKMLTEIDYMNVNPNKWTTDKAKNELIKTVIQNYILKWKEIDAEFKRKKFNVTIGDEL
                     PAGIMQLAKVFIAKKRKLKVGDKMAGRHGNKGIVAKVVRDEDMPFLEDGSPVDIVLNP
                     LGVPSRMNLGQIYETVLGWAGQKLGVSFATPIFDGATLDDITVYTEKAGLPRFGKTYL
                     YDGGTGERFDQPATVGVIYMMKLGHMVDDKMHARSIGPYSLITQQPLGGKAQFGGQRF
                     GEMEVWALEAFGASHILQEILTIKSDDVVGRAKAYESIVKGEPMPEPGIPESLNVLLH
                     ELRGLGLSINLD"
     gene            20158..24438
                     /gene="rpoC"
                     /locus_tag="PK839_06635"
     CDS             20158..24438
                     /gene="rpoC"
                     /locus_tag="PK839_06635"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HOG72445.1"
                     /translation="MSFRKDNKAKSNFSKITISLASPEEILERSSGEVLKPETINYRT
                     YKPERDGLFCERIFGPVKDYECHCGKYKRIRYKGIVCDRCGVEVTEKKVRRERMGHIT
                     LVVPVAHIWYFKSLPNKIGYLLGLPSKKLESIIYYERYVVVNPGIKAADGVKYLDFLT
                     EEEYLEIIDSLPKDNQHLDDNDPNKFIAGMGAEALNLLLSRLNLDELSYDLRNRANTE
                     TSQQRKSEALKRLQVVEAFRESGTINKPEWMILKVIPVIPPELRPLVPLDGGRFATSD
                     LNDLYRRVIIRNNRLKRLIEIKAPEVILRNEKRMLQEAVDSLFDNSRKSNAVKTDANR
                     PLKSLSDSLKGKQGRFRQNLLGKRVDYSARSVIVVGPELKMHECGLPKEMAAELYKPF
                     IIRKLIERGIVKTVKSAKKIVDRKDPVVWDILENVLKGHPVLLNRAPTLHRLGIQAFQ
                     PKLIEGKAIQLHPLACTAFNADFDGDQMAVHLPLSNEAILEAQILMLGSHNILNPANG
                     APITVPSQDMVLGLYYITKPRKGVKGEGLIFYSPEEVIIAYNEHAVDLHAIIKVRLML
                     PDKNGQLTPQLVETTVGRVLFNQNVPAEMGYINEVLTKKSLRDIIGAVLKKAGTAKAA
                     KFLDDIKDMGYYMAFKGGLSFNLDDVVIPPEKAQLIEEGYAQVDDVMANYNMGLITNN
                     ERYNQIIDIWTHTNAKLTLTLLKTLSEDNQGFNSIYMMLDSGARGSKEQIRQLGGMRG
                     LMAKPQKSGSVGNEIIENPILSNFKEGLSVLEYFISTHGARKGLADTALKTADAGYLT
                     RRLVDVSQDVIITENDCGTLRGLVATAIKKNEDVVETLYERILGRTTVHDVFHPLTGE
                     LIVAAGEEITEEIASIIEDSPIEQVEIRSVLTCESRKGVCSKCYGRNLATGRMVQIGE
                     AVGVIAAQSIGEPGTQLTLRTFHVGGTASNIISDSKIIARFDGRAEIEELKTITTEDE
                     LGQPYSIVIGRLAEMRIVDLNTGITLSTHNIPYGSKLYISNGDTIKKGQLICEWDPWN
                     AVIVSEVSGRVEFDALIKEITFREESDEQTGYREKVIIETRDKSKNPAVKITDEKGEG
                     IRMYNLPVSAHVVVDEGTVIRAGDIVAKIPRAMGKSGDITGGLPRVTELFEARNPSNP
                     AIVSEIDGEVTFGKVKRGNREIMVTSKTGEVKKYLVPLSKQILVQENDYVKAGMPLSD
                     GAITPTDILAIKGPTQVQEYIVNEVQEVYRLQGVKINDKHFEVIVRQMMRKVEIDDPG
                     DTRFLEKQVVDKWEFLEENDYIFDKKVVIDPGDAPNYRPGQIITARMLRDENSALKRK
                     DLKPIQARDAIPATSSQVLQGITRAALQTNSFMSAASFQETTKVLNEAAVRGKVDNLE
                     GLKENVICGHLIPAGTGLREFDRIIVYSKDEHEFVEKAGKSVSLTSND"
     gene            24828..25145
                     /locus_tag="PK839_06640"
     CDS             24828..25145
                     /locus_tag="PK839_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HOG72446.1"
                     /translation="MPEKMNPINIEINDEIAQGIYSNLAVITHSSSEFIIDFVRVMPG
                     IPKAQVKSRIVLTPDHAKRLMLALQENIGKFEAANGTIKLPEGSEGMPPFPINFGGKG
                     GVA"
     gene            25336..27405
                     /locus_tag="PK839_06645"
     CDS             25336..27405
                     /locus_tag="PK839_06645"
                     /inference="COORDINATES: protein motif:HMM:NF022039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 protein"
                     /protein_id="HOG72447.1"
                     /translation="MKKFADYTLMALLAGMLACSNNNEQLVTSPSGEVKVIFSLTADK
                     QPSYRVYFKDSPVIETSTLGFDFKDAPSLSKGMRVDSVLSNEFNETWELPWGEQRLVK
                     NHYNEMKVFLSEKKSPNRHFAVTYRVYDDGIGFRYEFPQQESMGDVTITDENTQFKLT
                     GDHLCWWEPGDWDIYEHLYNTTSFSKIDATKKRNHRDLAQTYIPENAVNTPVTMKTSQ
                     GLYLSFHEAAVNNYAGVTLKVDTVNYSMTTELVGNDSGVKVVTKTPFNTPWRMIQLAE
                     KPGDLIESRLILNLNEPNKLGDVSWFKPTKYVGIWWEMHLGKSSWDMASGKHGATTAN
                     AKRYIDFAAKNNIGALLVEGWNTGWEHWIGFDDREGVFDFVTPYPDYNLKEVVEYGRK
                     KGVDLIMHHETSSAVTTYDKQLDTAFALISSLNLHMVKTGYVGKIIPKGEYHHGQWMV
                     NHYRRVLEVAAKHKVAVDVHEPFMATGERRTYPNFVSQEGFRGQEFNAWSSDGGNPPE
                     HLAIIPFTCLLAGPMDYTPGIFNTKLKPYKPNNQVNNTLAQQLALYVVIYSPIQMAAD
                     LPENYENHPAFQFIRDVGVNWEQTRVLNGEVGDYVTIARQERGTNRWFIGAVTDENSR
                     ELSVKLDFLEPGKKYSATLYLDAPDAHWDKNPSAYEIKKMEVDSSTELNLYLAPGGGA
                     AISLFPL"
BASE COUNT         7812 a         5936 c         6459 g         7260 t
ORIGIN      
        1 agtgattttt acacctactt tgccgaccga accatgcgga ttgactaccg ccattcaggc
       61 gatgacaaaa ccgaaagttt cgaaatggcc aaaattgctt ctgaaggtag ctgggcaggc
      121 agaaccaacc atcttaacga cccatggaag cttggacttt actttttcga ggtaaaagat
      181 gctgccagtg gcaaaactat ctactcgcag ggtttctgca gcgtttttgg cgagtggcag
      241 acaactgccg aagccaaggc cggtgttaaa acctttaacg aaaccattcg gttgccctgg
      301 ccaaaaaatg aggttaacgt ggtgctgcag aaaagggatt cgcttaaccg gcttgtgcct
      361 gtttggaact taacggttaa tccggttgaa tacgaagatc tgatacccac aaggccatct
      421 gcttttgaac ctaaagtttt gctggataat ggcgatccaa aatttaaagc cgacattgtc
      481 atccttggcg atggctatgc tgaaaatgag gactcccttt tttggaagga tgcacagcgg
      541 ttttccgacg cattttttag cgttgaacct tttaaatcga gaattgctga ttttaatgtc
      601 agggcagtgc ttggaaagtc aacgcaaagc gggattaggc ttcaacgtaa ggatttttat
      661 cccgataacc tgctaaaaac cagctattat gcttttggaa ttgagcgcta cgtgctcgca
      721 tccgacgatt ggattgttaa ggactacgcc tctattgttc cctacgattt tatagtaatt
      781 ctcatgaatt ctgataagta tggtggtggg ggcattttca acctgtatat taccgccgct
      841 gcccactcca gagcatccga atacgtgatg gtgcatgaga tggggcacca cattgccggc
      901 ctagccgacg agtactacac cagcgacgtg gcttacgagc ctgccaacac caaggttgaa
      961 ccctgggagt ttaatataac agcccttata aatcccgaca gcctgaagtg gagggatctc
     1021 gtagcccctg gtacacctat accaactcca tggaacaagg tggagtatga gcagggtggc
     1081 ggtaacaaag tgctgctttc tgaccctgcc gccggaaagg taggggcata cgagggggca
     1141 aactacctta gcaagggaat gttccgtccg gaggtcaact gcatcatgat tgccggaaag
     1201 gaattttgcc aggtttgtaa acgagggatt aacagggttt tggaccttta ttgtgagtag
     1261 gcagctgtct gaactgctac aagcctacta ctataaaaat acccattctt ttttaccagc
     1321 atggtttttc tgctgtatgg tcggtaatta agggcattca atacctattc taccaaaata
     1381 tacattaata ttttttacct atagatagca gggtagaata actttatggt tttctaaaaa
     1441 attcagctga aaaatttttc attcaaaaaa ttttgtaact tccatcggta aaataactta
     1501 tgagaaatgg attccaacgt tctggtttcg ttgttggtca tcctgcttcc attgagtttt
     1561 atctccgagc ggataaccaa cctggtaaaa ctattcctgc caacctccaa ctttttgtca
     1621 attgggaatg tacgctacag ggagaacaac cccctactgg agaaaaagcg tgaacgcaga
     1681 atattcctga tcagcctttt atcgggcgaa ttggttgcct tctcactcaa ggcagacctt
     1741 tttactattc tgaatagcgg aaaatttggg tgggacgagc ctgcatcggg cttttcgtgg
     1801 attattggct gcctgtttac cggcttcttt ctgagctggg ggagcaaatt ctggcacgat
     1861 ttgctgggta ttatcctaga ggccaaaaac atgcgtaggg ctattattga cgagaaaaac
     1921 cttgatgtac agcttagggc ttttggtaca caagaaacta gcagtccatc tgaggaagtg
     1981 gctgctgtta cgatcaagcc taatcaacca caagcagcta aaccagaatt cgtgcaggat
     2041 aagcctacac ttcagcgaat tgccaagctg catcccctgc ttcgcgagga ggccaaggag
     2101 atttatttgc agatactcgc ccagggcatt gccgttcgct tcaccgatac gctgaggacc
     2161 tttgccgaac aggatgagct gtatgctaag ggaagaacca aacccggaaa aatagttacc
     2221 caggccaaag ccggacaatc gtttcataac tatgggcttg cccttgattt ttgcctattg
     2281 ttggctgacg gaagggaggc ttcgtgggac aggcacaccg atatcaacct gaatttaaga
     2341 tacgactggg atgaggtggt tgctcttttc aggcattatg gatgggaatg gggaggcgac
     2401 tgggttaact ttaaggatta tccccatttt cagaaaactt tcgggctatc aaccgcccag
     2461 ctgctgaagc tatataaccg taaggaggtt gatggagagg ggtatgtcgt tattaaagag
     2521 aatgaattta gctagctggc gttaataatt tttcttaatg tagaaacttg cagctccgat
     2581 tcatactgtt accgtcatac tttttacaaa agagcttcag gaaaccataa tatcatagta
     2641 aaatgggcaa aagaaaagta tttcttttca ttgctgctgc tctactggct atttgggttg
     2701 taggagttgt tagagatttc gataagcttt gcaagaaaac tcccaatccc gaataccaga
     2761 actttattac acagcaaaag cagaatttac aaaggacagt tgagcagctg acaaccggtg
     2821 aggtaattag agttgatggc gccaacttgt atcgaagcga caactgcatt ggctctaact
     2881 ttgaaggggc tgaggtgaag ctggctggca ttcagctata cgacagcata tatggcagta
     2941 agtctgttaa accctactat acttacagct gcaacggcgc cattctcgat cccgatcatc
     3001 cccttttcgg gctacctgat atagaggtgc ttattggtcc ggtaacctac tacgaaccgg
     3061 aactttcggg agtgcaagcc cttatgaact ttggctttag cagggatcct aaaccacata
     3121 agaccttccg taaagctcta tacgatagca caggtaagaa gctacaggtt tttgagatgc
     3181 agctttggct aacctatttc aaggttgcta tcagcgttat atccaaccgc gataaaccgc
     3241 agatcacccc agacgatgag tcgctgaatg cccgaatata cccggggaaa tggtactgta
     3301 gctcaaaacc tttgatatcg gttaacgatc tggagcaaaa ggaagagtac aaaaaccatc
     3361 agtatggcga tataaccttt gtgctagagg tcaaccccaa cgcttcgccc tggtacatta
     3421 ataccggaag cgttcatact gccagacccg acattgctgt tggggcagtt atctgtaagg
     3481 agctggttaa gcgtccggag gatgagaaca tgatgcacct tcaaacccag cggggaatgc
     3541 ttacacccct ctacttcaaa ccctttgagc gtaccgttca acccgagaaa attgatagca
     3601 ttgccggcca ggttgaaacg gagattcagg actataagaa tttcgatatt tggaacaaac
     3661 cgtactacgc cagactgtac tcacgaaata tcggctcccg acagaaaggt tttttcggac
     3721 agaaaaaatt tgacgaacag gtagaataca cattcttgct gccattgctc gttgttggaa
     3781 gttgggatgt tcagctcccg tgggatgtaa tgcccgaaat gcaagcacct gttccctatt
     3841 acaggggttt taacttgaaa aatcttttcc cggaatgggg tataaaatct ggtggctgga
     3901 taagtgccgg tttacttgtt gctatcgtca tactgctgct gctttggttt ttgcctatgc
     3961 tccgacgtta ggtttcatga tttatcaagg caacttgctt tcgcctgaac tccgaaagca
     4021 ctatggcggt tgccatagct acatttaacg attcaaccct ggctctgcct ccagcaaaat
     4081 cgggaatatg aattttttgg gtgatgcagc tcccaacttc ggggcttatt ccgttgcctt
     4141 catttcccat tacaatacaa ccgctctcca cccctggtaa atcgtaaatg ctttcaccct
     4201 caaggtaggt gccaaaaatt ggactgtttt tactgtaagc cgacagtttt gttggtaaat
     4261 tgcagtagtg aacctttatg cgggcaatag cgcccatact tgcctgaact accttaggag
     4321 agaaaatatc tgccgtatcg ttggagcata gaatggtgtc aatcccaaac cagtcggcca
     4381 aacgaagaat ggttcccatg tttcccggat cctgaatgga gtcgagggca agtacaagcc
     4441 ggtttgcatg gttaatgggc tctgcgttgt acaggggcat ttttaccaga gcaagaacag
     4501 gcgttggagt gctaagtgcg ctgatacgat tcatctcatt ttccgatacc agggtagtag
     4561 caatttttga atctaccgta aacatttttt caagattccc ggtatggtag atttcagcta
     4621 catcaagatg agatttcaga agttcagaaa ccagccgttg cccctcggca acaaatagtc
     4681 ccagctcatt gcggtgtttt ttttgtcgta acgatacgat tcgttgaata tttctcctgg
     4741 tgagcatagg tacgattttt cgctgtaaat atagatataa ttttatcccg aaaagctgta
     4801 aagtgttgta tgtaagagta ctttatggag tattcggctc atgtactcta cgaagtttat
     4861 tcagcacaaa acattaaccg ctggtcaaac aaagttcgct tgtgctgtaa aattatgcta
     4921 attgccatgt attgtaaaaa cgattttata aatttccgag ccatttatag tcagttagag
     4981 gaatgcattt tttgaggatt ttcagtagga tacgtaaaga atttatagct tacaggctta
     5041 tacctgtaat gctgggagtt gtttttgtat ccggttgcag cataacacga aatgttcccg
     5101 aggacgggta tttgctgaac aaatccaata taaagaccga cagcaaggag atttcaaccc
     5161 gcgacatgga ggattaccta aagttaaagc ccaacaagaa aattttaggt tttcgctttc
     5221 acttacgcct atacaacatg gcaaatcctg aaaagaataa gggtgtaaac aagtggctta
     5281 agaatatcgg ggaagagccc gtaatcatgg atactaacct tatagctgag ggttcgagaa
     5341 atctcctaaa ctaccttcaa agcaagggat tctataatgc gaaggtggaa gattcaatat
     5401 actaccataa aaaaaaggca aacgtaatct actccgttta ccccaacgtt ccctaccgca
     5461 taaaatccct aagctacctg gttgaagatt cagcagttct tgagttggta aagaaagaca
     5521 ctgccaattg ccttataggt gttgggaaac gcttcgatac cgatatgctt cagcaggaga
     5581 gaacgagaat agaagttttt ctgaaaaata atggatacta cagctttagc aaggactata
     5641 ttacgttcac tgctgatact aatttccgta gccacagcgt aaaccttatc ctcaccattc
     5701 gtaacccttt tacaattgat gaaaagggga ataaggttaa gtcgaatttt aaaaagttta
     5761 aagttaagcg ggtgtttatt tatccaaatt ttgagcccct agaatactta acgtatcaac
     5821 aacaggcaac gcttgatacg gtttactaca aggacataca gttcattttt tcgggtgatg
     5881 ccggagttaa cctggctgtt atttacgaaa atattcttat tcgacccggc ttaacctatt
     5941 ccagcgaggc agtcaaaaaa tcacaagcca accttagctc cataaggcta tttaaatttg
     6001 taaacatttc gtttaaagag gctaaggaag ccgaatttcc tattagccct ttcgattttt
     6061 tggatgagga cagtgccagc tccgattcca ccgagtatgc ctatctcgat tgctacattc
     6121 aacttaccca gaatacgctg caaagctacc aggttgaact tgtggggact aacaccaccg
     6181 gttcgctcgg tgccgaagct aacctttcct accagcacaa aaacctattc aggggtgccg
     6241 aggtgtttga ttttaagctt aggggcctgg ttgaaacagc ccagcaaaag ataaattttg
     6301 ataacaccct agaacttggc ggctccatgg ggctgagcat acccaagtat attggccctt
     6361 tttcttccag ggttcaggtt agccggtcgg ctcccaaaac ccaggtaacc gcatcctaca
     6421 gctaccagca tcgccccgat tatactcgaa ccattgccag cttgctgttt ggctatagct
     6481 ggagaagcgg aagctttatt acccacaacg tcaacccagc cgaggtgaat gccattacca
     6541 taagccgtat tagtgatgat tttaaggcct acattgatac aacttcgctc aagtatagct
     6601 atatcagtca gattgttacc gtgtcgagtt acggctttac ctttaacaac cagaatcccc
     6661 agaagcccgg tagctacacc taccttcgtt acaactttga actttcgggt aaccttctta
     6721 accttgcttt tgcagccttt aaccaaccta aaaatgccga tggttcttac agtatattca
     6781 atacaaattt ttcacaattt gtccggagcg atttaaacta tacctacaac cagtatgtag
     6841 atgcaaataa ctcgtttgcg tatagaattt ttcttggcgt aggctacccc tatggcaact
     6901 caaaagcatt accattcgaa aaacggtact tcaccggggg cgcaaatgga ataagggcgt
     6961 ggcaggcaag agcgctagga cctggatcat actatcaggc aaaagaacgc taccctaacc
     7021 gtacggccga tattaagctg gaggctaact tagaatacag gtataagctc ttctggaaag
     7081 tagagggtgc cttgttcata gatgccggca acatctggtc tatgcccgga attgacgaca
     7141 ggaaaggtgc aacatttcat ttcgataagt tttacgaaca gctggctttg ggtagcgggt
     7201 tgggatttag gatgaatcta ggtttcctga ttcttagaac cgattttggc tataaaattt
     7261 tcgacccggc aatcaaccct ggtaaaccat ttaggccatg ggttcccctg cagcaaaaat
     7321 ttatgtggag ggatattacc ttcaactttg gtattggcta tcctttctaa gattttgttt
     7381 tataccctat tttttatagt tttgtataaa ccattataat ctcaatgcca tgagttacaa
     7441 tagaattgtt gagctgctcg gcgataagtc cgaatttctt ctaaaccaca agtgtgaaac
     7501 ggtgaagaag gaacagctgc acttgcccgg tcccgatttt attgaaaggg ttgttgcact
     7561 atctaaccgg tcacctcagg ttttacggaa ccttcaggga atgttcaacc atggaaggct
     7621 tgctggcaca ggttacctgt ccattttgcc ggttgaccag ggtattgaac atagcgctgg
     7681 tgcatctttt gccccaaatc caatatattt tgatccggaa aatattgtaa ggcttgctat
     7741 tgagggtggt gcaaatgcag ttgcctctac ttttggagtt ttagcatcgg tttcccgaaa
     7801 gtatgcccac cgcatcccct tcattgttaa gcttaaccat aacgaactgc ttacctatcc
     7861 aaacaagtac gatcaaatca tgtttggctc ggtcgaagag gcatggaacc ttggtgctgt
     7921 tgccgtgggg gcaacaatct actttggctc ggaagaaagc acccgacaga ttattgaagt
     7981 ggctcaagcc tttgagcgtg ctcacgagct aggtatggtt accgtccttt ggtgctacct
     8041 tcgcaacaat gcgtttaaaa aggacggggt ggactaccat gttgctgccg accttaccgg
     8101 ccaggcaaac catctgggag ttacactaca ggctgacatc ataaagcaga aagcacctga
     8161 aaacaatggg gggttcaagg ctattggatt tggaaaaacc agcgataagg tttacagcca
     8221 gctttcatcc gatcatccca tcgacctaac tcgttatcag gttgttaact gctacaatgg
     8281 acgtgctggc cttatcaact cgggaggtgc ttcgagcggt tcgagcgatc tcgcagaggc
     8341 cgtatttaca gctgtagtca ataaacgggc cgggggtatg ggcttaatca gcggcagaaa
     8401 agctttccag cgccctatgg atgaaggtgt tacaatacta aatgctattc aggacgttta
     8461 ccttgaaagc aagattgata tagcgtaaat caacttcata ttttctgtaa acccacaact
     8521 ttgttgttgt gggttttttg ctaatacccg agcaaacatt tgcatttatt ctgctaattt
     8581 tataaaattg tttaccagaa gcttaagctt ccgattttga gaatgcattt atccgtgttg
     8641 atttattgta aatattgttt tctggctgca ttactttaag ctggttctgg ttgggttgat
     8701 tgctcctttt tacatagccg gcaggttgcc atgaataagt ttaaatcaaa attgaaagac
     8761 tacttttcat ttacccggag cgagcgcaac ggaacgctag tactatcatt tctgcttgtg
     8821 gtttttatgc ttgctcccgc agcaatcagg catataaagt ttagcaggaa tagcccgagt
     8881 agcactatga ataccgctat tgacagcttt ttcctttctg cccggtttaa ccctagaaat
     8941 gaggttgaaa aaacaacttt ttctattgcg cacgaggagg ttccacgacg agctgaaccc
     9001 aaacagtttc cttttgaccc caatacaatt tcactggata gcctggtgga gctcgggtta
     9061 tcggtaaagc aggctggggt gattttaaag taccgtgaaa agggtgggca cttcgattcg
     9121 gccaccgacc tcgctaagat ctacgtgctc gattcctcaa cgctggccag gctgaaaccg
     9181 cttgtcagga ttactgccaa atcgcatgaa cagcaactct tgaacgctga aagtggatca
     9241 agcagtattt tccctgtgga gctgaatact gcagacacca cccagctaat gcggataaaa
     9301 ggtataggca gaagctatgc caaccgaatt gttacttttc gaaatctggt tggaggattc
     9361 tacagtgtta accagctagc tgatatttac gggataaccc ccgagctggt agcctcaatt
     9421 tcgccccagg taaaggttga cagcctggcc atcagaagaa taaaccttaa cgaggtaacc
     9481 tacgaggagt tgaggagcca cccctacctt accgactatc aggctaaggc aatcatttac
     9541 taccgcggca aaaagggttc aattggcaat ccgcacgagc tggttcaaaa caagctggtt
     9601 ccggcagata agtttcaaaa gatatacccc tacctgataa ccgtgagata ggggttaaga
     9661 aaatgggcca attcaaatat atcaaggatt tcgcagctgt taattttggc attaattcaa
     9721 aaaaataatt tttattgatt tgaaaagtta aagaaaatat gtacttttgc aaatcaaaaa
     9781 actagtggta tgatcatagt tccaattaaa gagggtgaaa atattgaaag ggctctcaaa
     9841 aagtttaagc gcaaatttga aaaaaccggc attatgagag agttacggtc gcgtcaggcc
     9901 tacactaaac catcggttcg tcgcagggaa gaaattaaaa gagctattta tattcaacag
     9961 ctccagcaaa aggaggagta gctctcaata tttgttttgg gtaatcaggc attattttgc
    10021 ctgatttttt gttttttgag ttgagaatgc ctacattgca tgaaatgaat atgctggcat
    10081 cctttctgaa acatattgag gttgagagag gttactcctc gcataccgtt cgctcctatg
    10141 gggatgacat caggcaattt ttgaattttt accagccgga ggcaagcgag gtggattttt
    10201 ctcaaattac ccatcggcat attagggcat gggtagtgtt tctaatgtcg gaaggcattt
    10261 cagagcgatc gatcaaccgt aaactttcat cgcttcgcac ttttttcagg tacctgaaaa
    10321 atcaaggtgc tattagttct aacccaattg tcaaggttgt tccacccaaa gcaggcaaac
    10381 gtttacccga attcgttcct gaatctgaca tggaaaagct ggaagctgat aacctatttc
    10441 ccgcttcatt tgagggaatt cgcgacagcc ttattgttct cctactttac tacagcggaa
    10501 tacgactatc ggagctggtt ggcctgtcac tctcatcaat cgataccaac tcccaaacca
    10561 tgaaggtgct tggaaagggg aataaggaaa gagtggtacc cttacatccc gatattaaac
    10621 ctttaattca acggtatata gccgccaggc aagaagtagt agctgatggg tgtagtgcat
    10681 tttttataac ctgtaaggga aagcccattt acccgaaact agcctatcgg gtagtgaacc
    10741 gctacctgtc catggtaact ccccttcaaa aaagaagccc ccacgtgctg cgacacacct
    10801 ttgctaccca cctgctcaac cagggtgcag aactgaacgc cattaaggag ctactaggcc
    10861 attcaaacct actggcaaca caggtttaca cccacaatac atttgaaaag ttgaaacgta
    10921 actataatca agctcatcct agagcttaaa atttaaaagg aggaaccgat atgaacatca
    10981 aaatccagtc gatcaagttt gatgcggaca agaagcttac cgattttatt cacaaaaagg
    11041 tatcaaaact cgaacgcctg ttcgataaca ttgtggaagt tgaagttttc ctgaggcttg
    11101 agagcagcca ggttcaggag aataaaattg ccgagattcg tataaaagtg cctgggtccg
    11161 acttatttgc cgaaaaaagg tgcaaaacct tcgaggaggc tgttgatgag tgtattagcg
    11221 ccttaaaaat tcaggtaaaa aaacataagg aaaagcttag aggagaatag ctgttagcga
    11281 aagcccctga tacggggctt ttttgcattt gaactttaag tgccacattt aaaaaaaagg
    11341 tgtacagctg gcattaacct taataaaatt caaaattatt tttgcaggta attaaaaata
    11401 ttatacattt gcaatccgat tttagggggt aaatgttctt tgagtcattc tacacctttt
    11461 attgccgatg tagctcagtt ggccagagca gctgatttgt aatcagctgg tcgtgggttc
    11521 gaatccctcc atcggctcgg ggtgaaaaat ggggagatac caaagtggcc aactggggca
    11581 gactgtaaat ctgctggcgt acgccttcgt aggttcgaat cctgctctcc ccacgagtta
    11641 attgtgttgt tattggtttt gtcttttttt gatcgctttt tccagggaag gccttaacca
    11701 gtagccaaat gcgggagtag ctcagtcggt agagcatcag ccttccaagc tgagggtcgc
    11761 gggttcgagt ctcgtctccc gctctgcaca caatgccgat gtagctcagg ggtagagcac
    11821 ttccttggta aggaaggggt catgagttca attctcatca tcggctcgat tttttaaaca
    11881 tttgaaattc aagtttttaa atactaattt aattaatacc tgaagttatg gctaaagaaa
    11941 aatttgatag gtcgaaaccg cacgtaaatg ttggaaccat tggtcacgtt gaccacggca
    12001 aaacaactct gactgccgca attaccaaga tattggctga aaaggggctt tcagaatttc
    12061 gttcattcga ctctattgac aacgctcccg aggaaaagga gaggggtatc accattaata
    12121 cctctcacgt tgagtaccaa accgagaaac gacactacgc tcacgttgac tgccctggcc
    12181 acgccgacta tgttaagaac atggtaactg gtgctgccca gatggatggc gctattcttg
    12241 ttgttgccgc tactgatggc cccatgcccc aaacccgtga gcacatccta cttgcccgcc
    12301 aggtaaacgt gcccagaatc gtggttttcc ttaacaagtg cgatgccgtt gacgatcccg
    12361 aaatgctcga ccttgttgaa atggaagttc gcgagctgct cacattctac cagtttgacg
    12421 gagaaaatac tcctgttatc aggggttctg cacttggtgc gctgaatggc gaacccaagt
    12481 gggtggaaaa ggtaatggag ctgatggctg cggttgacga gtatatcccc attcctcccc
    12541 gtgaaaacga aaaacccttc ctgatgcctg ttgaagacgt cttctcaatt acaggccgtg
    12601 gaactgttgc taccggacgt atcgaaaccg gtgttatcca caccagcgat gaagttgaaa
    12661 ttatcggtct tagccatgaa aagatgaaat cggttgttac cggcgtggaa atgttccgca
    12721 aaattcttga ccagggtgaa gccggtgata acgttggttt gctccttcgc ggtatagaca
    12781 aaaaggaaat taagcgcgga atggtggttg ccaaacctgg gtccatcaca cctcacacca
    12841 agttcaaggc tgaaatatat gttttgaaga aagaggaggg tggccgtcat accccgttcc
    12901 acaaccacta ccgtccccag ttctatctta gaactcttga cgttaccggt gaaatagttc
    12961 tacccgaagg tgtggaaatg gtaatgccgg gcgacaacgt aaccattacc gttgagctta
    13021 tttatcctgt tgctattaat aaaaaccttc gtttcgcaat tcgtgaaggt ggcagaacag
    13081 ttggtgctgg tcaggtaatt gatataatcg aataatactg ctattctggc aaaaggcatt
    13141 acctgccttg agccagccta cacgggtgta gctcaattgg tagagtagtg gtctccaaaa
    13201 ccattggttg ggagttcgag tctctccacc cgtgctaaat atctgaagat atgaagatta
    13261 caaagtactt tcacgaagtt tacaccgaaa tggtgcataa ggtttcgtgg cctactatga
    13321 aagaacttca atccagcgca ttaattgtca ttgttgcttc ccttttaatt gcgctggtgg
    13381 ttttcgtcat ggacatcagc tttgagaacc tgatgaaggt tatctaccgt atgcttaact
    13441 aaccagcgcc caacttggga aaggaatgga agaaaatgta aaaaaatggt atgttcttcg
    13501 ggcaatttcc ggtaaggaga agaaagttaa ggagtacctg gagaatgaga ttacccgttt
    13561 gaaattacag gacttcatct ctcaagttct tatccctacc gaaaaggttt accagattca
    13621 taacggtaag aaaatcagta aggagagaat tttttaccct ggttatgttc ttgttgaagc
    13681 tgcgctggtg ggtgaggttc ctcacatttt gaggaatgcc cccaacgtgt tgggattttt
    13741 aggcgacccc aaatcgggcg atcctatacc ccttagaact tcagaggtaa accgtattct
    13801 tggaaaggtt gacgagctgg ttgatagcga ggaggagctg aatgttccat tctttgttgg
    13861 tgaaacggtg aaggtgatag acggtccgtt taactcgttt tccggagtaa tcgaggaggt
    13921 aaatgacgaa aagaagaagc tgaaggttat ggtgaaaatc ttcggccgaa aaactccgct
    13981 ggagttaagt tttatgcaag ttgaaaaaga gtaactaact aaaagagcaa atgaattatg
    14041 gctaaagaaa ttgctggatt tattaagctc caggttaaag gcggcgctgc taatccatcg
    14101 cctccaattg ggcctgctct tggttctaaa ggggtaaaca ttatggagtt ctgcaagcag
    14161 tttaatgcca gaactcaagc tcaagcaggg aaagttttgc ctgttataat tactgtttac
    14221 aacgataagt cattcgattt tgttgttaaa accccacctg ttcccgttca gctcctggaa
    14281 attaccaagc agcagaaggg atcagctgaa ccaaacagga agaaagttgc ttcagtaacc
    14341 tgggagcagg taaggcaagt agcccaggac aaaatgcccg atctgaactg ctttaccctt
    14401 gagtcggcta tgaaaatggt agcaggcaca gcccggagca tggggattgc tgttaccgga
    14461 gagtttccgg ctaaataacc acttaacatc ttttgagcaa tgactaaact gacaaaaaac
    14521 aggaagttgg ctttatcgaa ggttgagccg aacaggcagt acaagctttc tgaggcagct
    14581 gccctgttga aggatatcac ctacacaaag ttcgacgctt cggttgacat cgacgtgagg
    14641 cttaatgtcg atcctcgtaa ggccaaccag atggttcgcg gggtggttac acttcctcac
    14701 ggaaccggca aacaggtgag ggtgctggtg ctttgtactc ccgataagga ggaagctgct
    14761 cgtcaggccg gagccgagtt tgttgggctt gatgagtata ttgaaaaaat caaaggaggg
    14821 tggacagatg ttgatgttat cattaccatg cctacggtta tgggtaaaat tggcgccctg
    14881 ggccgtatcc ttggacctag aggacttatg cccaacccta aaagcggcac tgtaaccatg
    14941 gacgttgata aggctgttaa ggaggttaag caggggaaaa tcgactttaa ggttgacaag
    15001 tttggtataa ttcacgcttc aatcggtaag gtttcttttg agcccgagaa aatagttgat
    15061 aatgccattg aatttcttaa taccattatc aagctcaaac ccacaacggc taaaggaacc
    15121 tacctgaaga gcatttacct ttccactact atgagccccg gtttggcaat cgataccaaa
    15181 tctatagctg ctgcgaatta ataaaaccgt ttacagaccc aattcactat gacaagggaa
    15241 gagaaaaaaa atctgattga tgcgcttacc gaaactataa accgctaccc gcatttttac
    15301 ctcaccgata cgtcggcgct taatgctgag gacactagca agcttcgccg ctcgtgcttt
    15361 cagaaagagg ttaaaatggt ggtggttaaa aataccctgt ttaagaaagc cctggaacaa
    15421 agcggcaaat ttgacttttc gccgctattt acagagttaa agggcaccac atcggtaatg
    15481 tttaccgaat cggggaatgt gccggctaag cttatcaagg agttccgaac tgaacatccg
    15541 aaacccttac taaaagctgc ctttgtagag gagtccatat atgtgggcga cgaacagctg
    15601 aatatgcttg catcgctgaa gagcaagaac gagctcattg ccgatgttat tgccctgctg
    15661 caatcacccg caaagaatgt tatcgcttct cttcaatcag gaggaaatat tctatctggt
    15721 gttgttaaaa cactttctga aaaggaaaaa taacttaaag tttgaaatca attaaacact
    15781 tatcaaaatg gcagatctca agaaatttgc agaagattta gttaaccttt cggttaagga
    15841 agttaacgag ttagctaaga ttcttaaaga cgaatatggt attgaacccg ctgctgctgc
    15901 tgtagctgtt gctgctgcac ctgctgctgg tgctggcgcc gctgcagccg agaaaactca
    15961 gtttgacgtt attttgaaag ctgccggcgg agcaaagctt caggtggtta agctggtgaa
    16021 agatcttacc ggactggggc tcaaggaggc taaagatctc gttgatgccg caccaaagcc
    16081 ggttaaggaa ggtgtttcaa aagaagaagc cgaatctctt aaagcacaac tcgaagacgc
    16141 aggagcagaa gttgaactta aatagcaaaa gcctatattg ggtaaaggtt tagaatctat
    16201 ccattagatt ctaagccttt ttattgttat atttttacag ttcaacaaac actaatccaa
    16261 tgtctaataa taacaataac aaccgaatta gcttttccag aacgaagagt caatacgaat
    16321 accccgattt tctggaagtt cagctaaagt cattcaccga attttttcag ctgggcacta
    16381 gccccgaaga acgcagacac gaagggctgt ataaggtatt tctggaaaat tttccgatta
    16441 ccgatacacg aaacaacttc gttctggaat ttctagacta ctttgttgat ccaccccgct
    16501 actccattga ggaatgcctt gatcgagggc ttactttttg cgttcccctg aaggccaagc
    16561 tcaagctcta ctgcaccgat cccgagcacg aggacttcga taccgttgtt caggatgtgt
    16621 acctcggaac cataccctac atgacgcccc gcggaacctt cgttataaac ggtgccgagc
    16681 gcgttgttgt gtcgcagctc catcgttcac cgggtgtctt ttttggacaa agcattcacc
    16741 ccaacgggac aaagctctac tcagcccgaa ttattccctt taagggctcg tggattgagt
    16801 tcgccactga tattaataac gtgatgtacg cctacataga ccggaagaaa aagctgcctg
    16861 ttactacgct gctccgtgct atcggctacg aaagcgataa ggatattctg gaaattttca
    16921 acctggccga ggaggttaag gttaacaagg ttaacctgaa aaaaattgtg ggtcagaggc
    16981 ttgctgcacg tgtgctgaaa tcgtgggttg aggattttgt tgacgaggac accggggaag
    17041 ttgtatctat cgaacgaaac gaggtaatac tcgaccgtga aacagtggtc gagaatgaac
    17101 atattgacac cattattgag tcgggagcta agtccatact tgttcataag gaaaatcaaa
    17161 ctttttcgga ctacgctatt atatacaata ccctgcaaaa ggatccctgc aactccgaga
    17221 aagaagctgt agtgtatatc tataggcagc tgcgtaactc tgagcctcct gatgaggcta
    17281 ccgcacgtga tgttatagac aagctgttct tttcggataa acgatatgat ttgggcgagg
    17341 ttggacgtta ccgtattaac aaaaagctca accttacaac ctcccccgat gtaaaggtgc
    17401 ttacgagaga agatatcatt gagattatca agtacctgat tgaacttgtc aactccaaag
    17461 cagaggttga cgacattgac catttgagca accgacgggt tagaaccgtg ggcgaacagc
    17521 tggctgccca gttcagcgtt gggctggcaa gaatggccag aactatccgt gagcgaatga
    17581 acgttaggga caacgaggtg ttcacgccta ttgacctgat aaactctaaa acgctttcat
    17641 cggttattaa ctcctttttc ggcacaaacc agctgtcgca attcatggat cagactaacc
    17701 cgctggccga aatgacccat aagcgtcgtt tatctgcact tggacctggt ggactttcca
    17761 gagaacgtgc cggttttgag gttagagacg tgcactacac ccattacggc aggttgtgcc
    17821 ccatcgaaac gcctgaaggc cctaatatcg ggcttatctc ttctctttgt gtttacgccc
    17881 gaataaacga tttgggattc attgaaactc cctaccggaa ggttgacggc ggaacggtgg
    17941 gcatgaacga tgacgatgta gtgtacctga ctgccgagga ggaggaggcc aaaatcattg
    18001 cccaggcaaa tgctcctatt ggtgatgatg gttcgtttgt tgaatccaag gtaaaagctc
    18061 gttacgaagg ggatttcccc ctggctgaaa gcgaatcaat cgaccttatg gatgttgcac
    18121 ctaaccaaat tgcctccatt gccgcttcgc tcattccttt tttggaaaat gatgacgcca
    18181 accgtgccct catgggttcg aacatgatgc gtcaggcagt tccacttcta aagcccgaaa
    18241 ctccgattgt tggaaccgga ttggaaggta aagtggtgag cgatagtcgt gtgcaagttg
    18301 ttgccgaaca ggatggcgtg gtggagtatg ttgacgccaa cgagattgtt gtaagatata
    18361 accgctcgga agatgaagtt tttgttagtt tcgaccccga gcttaaacga tatagcttac
    18421 caaaattcaa gaaaactaac caaaatacat ctattaccct aacacccatt gttaccaagg
    18481 gccaaataat taaaaaaggc gatatcctta ccgaaggcta tgctactcgc aacggagagc
    18541 tcgcccttgg gcgaaacctt aaggtggcct ttatgccttg gaagggttac aactttgagg
    18601 atgccatcgt aattaacgaa cggttgatta gggatgatat gtttacctcc cttcatattg
    18661 atgagtacat ccttgaggtt agggatacta aacgtggaat ggaggagctt acagccgata
    18721 taccaaacgt atcggaagag gctactaaga acctcgatga gaacgggatg atccgtatag
    18781 gcgccaacgt ggtgcctggc gatatcctta tcgggaagat cacccctaaa ggggagtctg
    18841 atcccactcc cgaggaaaaa ctgctacgcg caattttcgg agataaggct ggcgatgtga
    18901 aggatgcctc actaaaagct tccccatcgc tttacggcgt ggtagttgac aaaatgcttt
    18961 tttcaagggc tattaaggat gaaaaggacc agaaaaaatc caagtcaact gagaaaagta
    19021 agctggaaaa aatcgacaac gagtttaacc gtcaggttgc cgagcttcaa accaagctgg
    19081 ttgataagct gtttgttctt gtaaacggga aaacctcgca gggcgttgtg gataatttca
    19141 accaggagct cattcctcgt ggggtgaaat ttacacataa aatgcttacc gagattgact
    19201 acatgaatgt taacccaaac aaatggacaa ccgataaggc taagaatgag ctgattaaaa
    19261 ctgttattca gaattacatt ctgaagtgga aggagattga tgcggagttt aagcgcaaga
    19321 agtttaatgt aaccatcgga gatgagctgc ctgcaggaat tatgcagcta gccaaagtat
    19381 ttattgccaa aaaacgcaag ttaaaagtgg gcgataagat ggctggccga catggaaata
    19441 agggaattgt ggccaaggtg gttcgtgatg aggatatgcc gttcctggag gatgggtcgc
    19501 ctgtcgatat agttttgaac cctctaggcg ttccctcccg aatgaacctg ggtcagattt
    19561 acgaaaccgt tctaggttgg gcagggcaaa agctgggcgt tagctttgcc acaccgattt
    19621 tcgatggtgc aactcttgac gatataaccg tgtacaccga gaaggccggc ttaccccgct
    19681 ttggtaaaac atacctgtac gatgggggta ccggcgagcg ctttgatcaa cctgcaactg
    19741 taggtgtaat ctacatgatg aagctcggac acatggttga cgataagatg catgcccgtt
    19801 ctataggccc ttactccctt atcacccagc aaccacttgg tggcaaggct cagtttggcg
    19861 gtcaaaggtt tggtgaaatg gaggtatggg cgctggaagc ttttggggct tcccacatcc
    19921 ttcaggaaat actcaccatc aagtccgatg atgtggtcgg tagagctaaa gcttacgagt
    19981 caattgttaa gggagaacca atgcctgaac ccggcatacc cgagtcgctc aacgtgttgc
    20041 tacatgaact tagggggttg ggcttaagta ttaatcttga ctagcccgat tgcacagttc
    20101 tggctaaaga ctttttttgc tgtccagctg cataacataa cttttaacta gttgaatatg
    20161 tcgttcagaa aagataataa ggctaagagc aacttctcaa aaatcactat cagccttgcc
    20221 tcacccgaag aaatactgga gcggtcgagc ggagaagtgc taaaacctga aacgataaat
    20281 tacagaacct ataagcctga gcgagacggg ttgttttgtg aaagaatttt cggtcccgtg
    20341 aaggattacg agtgccactg cggaaagtac aaacgcattc gttacaaggg tatcgtatgc
    20401 gaccgttgcg gtgttgaggt tactgagaag aaggtacgca gggagaggat ggggcacatc
    20461 acacttgtgg ttccagtggc acatatctgg tattttaagt cgttgcccaa caaaataggc
    20521 tacctgcttg gattaccctc caaaaaactg gagtcaataa tctactacga gcgttacgtg
    20581 gtggttaatc ctggtattaa agctgctgat ggagtcaaat acctcgattt ccttaccgaa
    20641 gaggagtatc tcgaaattat tgattccctt ccaaaggata accagcatct tgacgataac
    20701 gatcctaaca agttcattgc cggaatggga gcagaagcgc ttaatttgct cttgtcccgc
    20761 ctaaacctgg atgagctgtc atacgacctt cgcaacaggg caaacaccga aacatcccaa
    20821 cagcgtaaaa gtgaagcgct gaaacgcctt caggtggtag aggcctttcg ggaatcgggc
    20881 accataaaca agccggaatg gatgatattg aaggtaattc ctgttattcc tcctgagctt
    20941 cgtcctttag tgccgcttga tggaggaagg tttgccactt ccgacctgaa tgacctatac
    21001 cgtagggtga ttattcgtaa caaccgtctg aaacgtctta ttgaaattaa agcccccgag
    21061 gtgatacttc ggaatgaaaa gcgtatgctt caggaggcag tggactcgct tttcgacaac
    21121 tcccgcaagt caaacgctgt taaaaccgat gccaaccgtc cgttgaaatc actgagcgat
    21181 agcttaaagg gaaagcaagg tcgtttccgt cagaacttgc taggtaaacg agttgactac
    21241 tcagcccgct cagtaattgt tgttggtccc gagctgaaga tgcacgagtg cggtctccca
    21301 aaggaaatgg ctgcagaact ctacaaacca tttattatcc gcaagctgat tgaaaggggc
    21361 attgttaaaa cggtgaagtc ggccaagaag attgttgacc ggaaggatcc tgttgtttgg
    21421 gacattctgg aaaacgtttt aaaggggcat cccgtgctac ttaaccgtgc tccaaccctt
    21481 caccgcttgg gtattcaagc attccaaccc aagctgattg aaggtaaggc aattcagctt
    21541 cacccccttg cctgcacagc atttaacgcc gactttgatg gcgaccagat ggcagtccac
    21601 ttgcccttga gcaacgaggc catcctggaa gcacaaattt taatgctcgg ctcacataac
    21661 attctcaacc ctgccaacgg agcgcctatt accgttccat cgcaggatat ggttcttggt
    21721 ctttactaca tcaccaaacc tagaaaaggc gttaagggcg agggcttaat tttctactcg
    21781 cccgaggagg taataattgc ctacaacgag catgctgttg acctacatgc catcattaag
    21841 gttcggctta tgttacccga taagaatggg cagctgaccc cgcagcttgt tgaaaccact
    21901 gttggacggg ttttattcaa ccagaatgtt cctgcagaaa tgggctacat caacgaggtg
    21961 ctaaccaaga agtcgcttcg cgatataatt ggtgctgttt tgaagaaagc cggcacggct
    22021 aaggctgcca agttcctcga cgacataaag gatatgggat actacatggc ctttaagggt
    22081 ggactttcat tcaacctcga cgatgtggtt atacccccgg aaaaagccca gcttattgag
    22141 gagggctatg cccaggttga tgacgttatg gccaactaca acatgggtct gattaccaat
    22201 aacgagcggt acaaccaaat tattgatatc tggacacata ctaacgccaa gcttaccctt
    22261 accctgctaa aaaccctttc ggaggacaac cagggcttta actccatcta catgatgctt
    22321 gattctggag ccaggggttc gaaggagcag atacgccagc ttggaggtat gcgtggcctg
    22381 atggccaagc ctcaaaagtc gggatcggtg ggaaacgaaa tcatcgagaa ccccattcta
    22441 tcgaacttta aagaggggct atccgtgttg gagtacttca tctctactca cggtgcacgt
    22501 aagggtttgg ccgataccgc tctgaaaaca gccgatgccg gttaccttac ccgaaggttg
    22561 gttgacgtat cgcaggatgt tattattacc gagaacgatt gtggaacgtt acgtggtttg
    22621 gtagctactg ctatcaagaa gaatgaggat gtggtggaaa ccctttacga gagaattctt
    22681 ggaagaacta ccgttcatga tgtattccac ccgttaaccg gtgagcttat tgtggctgca
    22741 ggcgaggaaa taaccgaaga aattgcctcc attattgaag attcaccaat tgaacaggtc
    22801 gaaattcgat cggtactcac atgcgagtcg cgaaagggtg tatgctccaa gtgctacggt
    22861 cgtaacttgg ccaccggtcg catggtgcaa attggagaag ctgttggcgt tattgccgcc
    22921 cagagtattg gagaacccgg tacccagctt acacttcgta cattccacgt tgggggaacg
    22981 gcttccaata taatttccga ttctaaaatt attgcccgct ttgatggcag agccgaaatt
    23041 gaagagctca aaactattac cacagaggat gaactcggcc aaccgtacag cattgtaatt
    23101 ggccgtctgg ctgaaatgcg tatagttgac ctgaataccg gtataaccct ttctacccac
    23161 aacattccct acggatcgaa actttacatt tcaaacggcg ataccataaa gaagggtcag
    23221 cttatctgcg agtgggatcc atggaacgct gtgattgttt cagaggtttc gggtagggtt
    23281 gaattcgacg cccttatcaa ggagattacc ttcagggagg aatccgatga gcaaaccgga
    23341 taccgcgaaa aggttattat cgaaacccgc gataagagca aaaaccccgc cgttaaaatt
    23401 accgacgaaa agggcgaagg cattaggatg tacaacctgc cagtttctgc ccacgttgtt
    23461 gttgacgagg gtacagttat aagagcgggt gatattgttg ctaagattcc tcgcgcaatg
    23521 gggaaatcag gcgatattac cggaggtttg ccaagggtaa ccgagctctt tgaagcacga
    23581 aatccttcta accctgcaat tgtttcagaa attgacggtg aagttacctt tggtaaggtg
    23641 aaaagaggta accgtgaaat tatggttacc tccaaaaccg gagaggttaa gaaatacttg
    23701 gttccactct ccaagcaaat acttgttcag gaaaacgact atgtgaaagc tggaatgcct
    23761 ctttccgacg gagctattac tccaactgat attcttgcca taaaaggtcc aacgcaggtt
    23821 caggagtata ttgttaatga ggttcaggag gtttacaggc ttcagggggt taaaatcaac
    23881 gacaagcact tcgaggttat tgtacgccag atgatgcgca aggttgaaat tgacgaccct
    23941 ggcgatactc gattccttga aaaacaggtg gttgataagt gggagtttct tgaagagaac
    24001 gactatatct tcgataagaa ggtggttatc gatcccggag acgctcctaa ctatagacct
    24061 ggacaaatta ttactgcccg gatgcttagg gatgaaaact ctgccctgaa acgtaaagac
    24121 ctaaagccca ttcaggctag ggatgctatc cctgcaactt caagccaggt gctgcaagga
    24181 atcacacgtg ctgcactgca aaccaacagc ttcatgtctg ccgcttcgtt ccaggaaacc
    24241 actaaggttt tgaatgaggc tgctgtaagg ggtaaggttg ataaccttga agggctaaag
    24301 gagaatgtga tctgtggaca ccttatccct gccggcactg ggcttagaga gtttgacaga
    24361 ataatagttt actcaaagga tgagcatgag tttgttgaaa aagcaggtaa gtcggtttcg
    24421 cttacgagca acgactagcc atagcaagaa aatatcctta aaagggttgg ctttactgcc
    24481 aacctttttt ttgtccttcc tccagcagta taatctaagc taaaagttgc tggctgcttt
    24541 cataatattg aagcacccat atatgggcaa gcgttacctt caatttcggc gaagcccaaa
    24601 tatggttgct ccatcagctc cacccccata cttatgagat atgagtcaat gtacttgtta
    24661 tcagaaacct aaataaatta tgcagggatt gaatgctgca taacggaaac gagttaacgt
    24721 tggtttaaaa attttaccaa agtaaaggcc actatcaccg atgctaatct ttgccgaatt
    24781 tgaaaaaatt actaactttc cgttctaaaa attaataata atacgccatg cctgaaaaaa
    24841 tgaatcctat caatattgag attaacgatg aaattgccca gggtatatac tcaaacctag
    24901 cggtgataac ccattcatct tcagaattca ttattgactt tgtgcgggtt atgcccggta
    24961 tccctaaggc tcaagttaag tcaagaattg tgctaactcc cgatcatgcc aagaggttaa
    25021 tgctggccct gcaggaaaac attggtaagt ttgaagctgc aaatggaacc attaagctac
    25081 ctgaggggag tgagggtatg ccgccattcc caataaattt cggaggtaag gggggcgttg
    25141 cctagtgttt gttggcattg gcattctttg tgcatatatt actgaaatac agtagtttag
    25201 attggtattt taatttcagg agtttggttc acctgaataa atgttgtagc ggaatactat
    25261 tttctaaaca ggtattcacg tttactttca aacttatata catatttgtt gggcaagata
    25321 aaagtaaaat ttactatgaa gaagtttgca gattataccc taatggcatt gctggccggt
    25381 atgctggcat gtagcaacaa caacgagcaa ctggttactt ccccatcggg agaagtgaag
    25441 gtaattttca gcctcactgc cgataaacag ccctcttaca gggtttactt taaagactct
    25501 cctgtgattg agacctcaac acttggattc gatttcaagg atgccccatc gctgtccaag
    25561 gggatgagag ttgatagcgt gttgagtaat gagtttaacg aaacgtggga gctgccgtgg
    25621 ggcgagcaac ggcttgtgaa gaatcattac aacgagatga aagttttcct gtcggagaaa
    25681 aaatcaccaa accgccactt tgcggttaca tatagggttt acgacgatgg aataggattt
    25741 cgttacgaat ttccccaaca ggaaagtatg ggcgatgtaa caatcaccga tgaaaataca
    25801 cagtttaagc taaccggcga ccatttgtgc tggtgggagc ccggcgactg ggatatatat
    25861 gagcatctat ataacacaac ttcgtttagc aagattgacg ccaccaaaaa gcgaaaccat
    25921 cgcgatttgg cacaaaccta cattccggaa aacgccgtaa atactcctgt aaccatgaaa
    25981 actagccagg ggctatacct tagctttcac gaagccgctg ttaataacta tgccggagta
    26041 accttaaaag ttgatacggt taactactcc atgacaactg agctggttgg caacgattct
    26101 ggagttaagg tggttaccaa aacacccttt aacactccct ggcgtatgat tcagcttgcc
    26161 gaaaaacctg gtgatttgat agaatcaagg ctgatactta acctgaacga gcccaacaag
    26221 ctgggcgatg tttcatggtt taagcccacg aagtacgttg gtatatggtg ggagatgcac
    26281 ctgggaaaat caagctggga tatggccagt gggaagcatg gggcaactac tgcaaatgct
    26341 aaacgatata tagactttgc cgctaagaat aatatcggag ccttacttgt agagggttgg
    26401 aataccggtt gggagcactg gattggattt gatgataggg agggagtttt tgacttcgta
    26461 accccctatc cggactacaa cctcaaggag gtggttgagt atggccgcaa gaagggtgtt
    26521 gatctgatta tgcaccacga aacatcatcg gcggttacca cctacgataa gcagctcgat
    26581 accgcctttg ccctaatcag cagcctgaac ctccacatgg ttaaaacagg atacgtaggg
    26641 aagataatac ccaaaggaga gtaccatcat gggcagtgga tggttaacca ctaccgaagg
    26701 gttcttgaag tggccgcaaa gcacaaggtt gccgttgatg ttcatgaacc gtttatggca
    26761 acaggcgagc gcagaacata tccaaacttc gtttctcagg agggattccg gggacaggag
    26821 ttcaacgcat ggtcgagcga tgggggaaat cctcctgagc atttagctat tattcccttc
    26881 acctgcctgc tggccggacc tatggactat acaccgggaa tcttcaacac caagctgaaa
    26941 ccctacaaac ccaacaacca ggtaaataat acactggcac agcagcttgc cctttatgta
    27001 gtcatctaca gcccaattca aatggctgcc gatttgcctg agaattatga aaaccatcct
    27061 gcattccagt ttatccgtga tgtgggtgta aactgggagc aaaccagggt gcttaacggt
    27121 gaagttggcg attatgttac cattgcccgt caggaacgcg gaacaaaccg ttggtttatt
    27181 ggtgcggtaa ccgatgagaa ttcaagggag ctcagcgtta agctggattt tctggaaccg
    27241 ggtaaaaagt attcagcaac cctttacctt gatgctcctg atgcccattg ggataagaac
    27301 ccctcggcat acgagataaa gaaaatggag gttgacagca gcacagagct aaacctgtat
    27361 ttagcaccag gaggtggagc ggcaatttcg ctttttccgc tataagtgag ggttgaaaat
    27421 aaaatatcag aagagtcccc ggccagtaac cggggatttt tttaacg
//
LOCUS       DAOSHM010000033        27343 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_1371_length_27343_cov_20.428393, whole genome shotgun
            sequence.
ACCESSION   DAOSHM010000033 DAOSHM010000000
VERSION     DAOSHM010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 27343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27343
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1371_length_27343_cov_20.428393"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(241..1077)
                     /locus_tag="PK839_06650"
     CDS             complement(241..1077)
                     /locus_tag="PK839_06650"
                     /inference="COORDINATES: protein motif:HMM:NF017536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HOG72448.1"
                     /translation="MITVRRVIPAQQVTDGAGVKLNRVFGYDTRMETDPFLLLDYFGS
                     ENPTDYAAGFPWHPHRGIETITYMLQGKVEHSDSLGNRGEVNPGGVQWMTAGSGIIHQ
                     EMPKPISGLMYGFQLWLNLPRSFKMVPPTYHDLKSSDIPEVETSSSRVRVIAGRFRKA
                     VGLSTPYNSSIDVLDVRLWPDALFDEALNANSSYFLFVYDGQISFADYSKPIGSLNGV
                     ILDKGSILQLKAESNGANFLLFGGQPINEPIAWRGPIVMNTNDELSVAFEEINQGTFI
                     KE"
     gene            1310..4375
                     /locus_tag="PK839_06655"
     CDS             1310..4375
                     /locus_tag="PK839_06655"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG72449.1"
                     /translation="MTKLRAFILSLTLAAAFSLAHGQETSISTREEALFAKAFELYQK
                     GLYASSQKTFQKFLSEYSSDVVAQLRTDAEFYSAICAIELNNNDAEYLLGRFIQNHPE
                     SQYIDLAYFVMGKRQYQQKNFTSSIYWLSMIDGEGLDREKRYEMQFMLGYCYFMTNDF
                     ELANKWLYQVKDMDSKFSGPATYYYSHIAYLQKKYATALKGFEKLQNDETFAPIVPFY
                     ISQIYYLQGDYQKVVAYAPPLVADNATRRTPEIARIVGDSYFKLRQYDSALVYLNKYV
                     EKTPQLSRDDYYLVGFTYYKTGNFSEAAKHLERVSTVDDSLSQNANYHLADCYLKLND
                     KPKARQAFGMAAKLDFDSFIKEDALFNFAKLTYEQLYAPFNEAIEAFRQYITLYPNTP
                     RTDEAYNYLTLAYLSTRNYKEAVDALEKIKVQDVTIKTAYQKATFYRGLELFQNMQFD
                     DAITMFAKSLENSAYNPTIAALATYWQAEAYYRLEQFEKAAEGYNRFILTSGAFSLPE
                     YLMAHYNLGYSNFKLKRYDEAVVWFRKFTNLSNDEKNVFVGDAYNRIGDSFFIQRRYW
                     AAIDYYDKAMAIGTIDADYALFQRGFALGLVERPEKKIESLQKLLTLYPSSSYADDAI
                     FEIAQSQLVLTQTGKAMENYKLIEKNYAGSSYYVRALVQLGLLYYNSENPDSAMMYFK
                     RVVEGFPNSPEAKNALMGIRNIYVDRGDANTYFNYASSLGSLANMSLAEKDSLTYISA
                     EKIYITGDCEKSNAAFSSYLRDFPQGNFSINALFYQGECYRKQGMRDRAIEAFDDVAT
                     RQKNDFTGQSLLRLGELYFEKGSYDKSYEAYAKLESVAEYKSMLQDARIGMLRCAFEQ
                     KQYADIPAIASSILSAEKLPDELERETQFKLAKSLYELGRADEALVEYSKVATNLKTA
                     EGAEAKYMKTSIYFQKKDYKKTEAEVFDFAEKNTPHQYWLAKSFILLAEVYANQDDFF
                     QAKATLNSVIDGYSSTTDGIIDEATAKLNQLVKTEKDKQSVKVDEGVTF"
     gene            4404..6203
                     /locus_tag="PK839_06660"
     CDS             4404..6203
                     /locus_tag="PK839_06660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72450.1"
                     /translation="MQSRKYTNKHTPHVEVCRLAAPYHLAKKPTILNTHKHIKQHFAP
                     NILLAGTLAFIFTTNLLVGNKAVAQETSLKKEVMVVRPYEPSISDAFKINQLPKIEDT
                     IRVTPNFTYSIVQRPAVTAFTPSPISAARMMAEPLKDLSKGYIKLGMGNAVLPVAEVY
                     FMSKRNPDFMYGAWVQYRSKIGKVKLENSKKVDASSSTADIQIFGKRMFANKILQGDL
                     GYSNNARTYYGYNILDSLTSPTSDKQRVNRVNANLNFNTSRKDSAHFNYNALASFEHL
                     GDKFDMQENRITGLGGLNKYLKQEQLGVDISFTHYSRSSQFDMQKNSILNISPWIHFF
                     GKQWRAVAGLYMVYDANSGINNTFIYPRAMLSYDVVSHYVIPYIEFGGYLEENSYSKL
                     IRENPWITPGISAWNTTHKLVMTGGVKGKFSAKVSYNVQASYSIVDSLYFYTNISINP
                     ANPLYNRFNLDFDNVELTKILGELTIAPSERFSVLIHTEYFSYKMNELAHPWHKPDYN
                     TYAELRYNLRDKIYTSIQLFAVGKRWVLSPDGVSPVRLKGYVDANLGFDYNFNNRLYA
                     FLNLNNITSSRYQEWYLYPVQGFNLQAGLSYKF"
     gene            6559..8994
                     /locus_tag="PK839_06665"
     CDS             6559..8994
                     /locus_tag="PK839_06665"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOG72451.1"
                     /translation="MLSMPRLAAQPAGFKEIFTDAEYYLLMDDYQEALPLYQKLYKLD
                     STNSNIHYRLGQCYLNISGKKQMAIPFLEMAAQSISPKYVEGSYRETKAPLNALFYLG
                     QAYMINMELSKALNAFTQFRENLNVGDIYNLDYVNQQIKACQTAASFLEKPLNIKKER
                     FVLLPGRNVSISYPVFSGDRQSLVFTVKEKFYTALYHSVKTNGEWGTPSNITLDLGVE
                     GEVYSTSLNHDGTKLLIFINDMGSGNIYISTLEGGKWQLAKKFQGDINSRDWETFASY
                     SADDKYILFTSNRKGGVGGLDIYISELLPGDKVSPPSNLGEIINTPYNEESPILSPDG
                     NTLYFASQGHNSMGGYDIFYSKRIPNGQWSNPVNIGYPINTPDDEYFYFPFDSSTAVM
                     PLASNGESPYNELYQISINPAPPVRYVGLDGSIYLTDNSDIQSADVKIIITSDSILNI
                     RDTIVPSKIGKFTCTLTPGTYRVEAASNFYSMEPVHLYIPRNFNQEVYPLELKLQPKM
                     VAEGAVIKLQNILFDFDSYALTREAKFELEKVFNLMAENPSLYIEVTGHTDSKGSPIY
                     NLKLSAKRASAVVDYLVAKGIDERRFIVRGLGSLENLATNTNPDGSDNPAGRQFNRRA
                     SIRIFNPDKKISIEYVDVPEHLKPINSRYTILLAQPGVKPTEKQISEVEKALDMNTQV
                     YAVGSYSFICIGSFSSKSDAIESFGKVVDLNFPQAVIINENELQRIVRSSQKQLITKR
                     TIYTILVVTCEKPLNQDSFKGFPVTEEKGEDGLYRYYFGIFDTRDSAKKNLDTVNSLG
                     FPNAIIVKLEKNR"
     gene            8994..9560
                     /locus_tag="PK839_06670"
     CDS             8994..9560
                     /locus_tag="PK839_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOG72452.1"
                     /translation="MTEGVLENNMVKLRAPEPDDVDLLYVWENNMEIWKVSNTITPFS
                     RHLLKKYIETAHLDIWESKQLRLIIEAKDQNALMNVPVGLIDLFDFDPYHLRAGIGIL
                     IANQQYRRRGYATEALKLLVRYCFGILQLNQIYCNISADNQVSLQLFKKQGFVEVGVK
                     KSWLKTQSGWEDEVMLQLLNPKPRLPKN"
     gene            complement(9625..11088)
                     /locus_tag="PK839_06675"
     CDS             complement(9625..11088)
                     /locus_tag="PK839_06675"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOG72453.1"
                     /translation="MSQTEKYFEQFRKNIVGIDTTYPTVFGQKPLIYADWIASGRLYR
                     PIEERMLNVFGPMVANTHTESSYTGKFMTEAYHFALKEIKRHVNAGSDDVIVTSGFGM
                     TSSVNKFQRILGLKNYSFVFANPKERPVVFVTHMEHHSNQTSWYETIAEVVVVEPDEN
                     LMVSPANLRRTLENYSDRPMKIGSFTACSNVTGIRTPVHELARIMHEVGGLCFVDYAA
                     SAPYEEINMHPSEPLERLDAIFFSPHKFLGGPGASGVLIFNSQLYKNRVPDNPGGGTV
                     DWTNPWGEYKYIDDIERREDGGTPGFLQAIRSALAIRLKEKMGVKNIALREEELVNLA
                     FEKLKGIPGLHILAENCCQRIGSISFYIDELHYNLVVTLLSERYGIQMRGGCVCAGTY
                     GHFLLNVTKDKSHRITEMINHGDFSQKPGWVRWSLHPTTTDAEVEYIANALREISANG
                     TSWKNDYTYCKHTNEFCSKNPEVKSLWPDIPSLFNFD"
     gene            complement(11119..11592)
                     /locus_tag="PK839_06680"
     CDS             complement(11119..11592)
                     /locus_tag="PK839_06680"
                     /inference="COORDINATES: protein motif:HMM:NF024976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HOG72454.1"
                     /translation="MAVEGISRLNFPEFDFRVREVGKSLQIFDAVRKKFVPLTPEEWV
                     RQHLIRYLCDFKNVPTGLIGVEVAFLLNGVNHRADVVVYGRAGKPLVVVECKAADVEL
                     SESTVDQVVRYNSYFKAPYLVITNGIKHYTFAVSWESGNSYSLNEIPDYSVMEQS"
     gene            11858..12583
                     /locus_tag="PK839_06685"
     CDS             11858..12583
                     /locus_tag="PK839_06685"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HOG72455.1"
                     /translation="MKRLLFTLVIVMAYGISNAQLFKVFGHEVGYIYVGPRLGMNFSK
                     FSDFNGTYEKSRFGYEFGAVGEFGFTTRFSIQTELMFLSKGSRDENFGKIRMNYIGIP
                     ILAKYGFRALGLDKVYVMGGTFANVRVGGAWVYDDGTTYELGGGMKKFEWGMGLGAGA
                     QYPFKTGVAALDLRYSLGFSDIHSDDNITTHSRSFEVILTYKFDLVDLMFKLKKNKTP
                     EPEPAGNEVDSNNAKGLKIEKRN"
     gene            complement(12652..13290)
                     /gene="folE"
                     /locus_tag="PK839_06690"
     CDS             complement(12652..13290)
                     /gene="folE"
                     /locus_tag="PK839_06690"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HOG72456.1"
                     /translation="MEEINQGASINDEEGYSRIERWNAETTEKLAYHYSEIIKLIGED
                     ASREGLVQTPVRVAKSIQFLTHGYNVDPHEMIRSARFKEDYRQMVLVKDIELYSLCEH
                     HMIPFYGKAHVAYIPNGYITGLSKIARVVDAYARRLQVQERLTVQIRDCIQETLNPLG
                     VAVVIEAAHMCMQMRGIQKQNSVTTTSAFTGAFLTHDNTREEFIHLIGVRLH"
     gene            complement(13304..13723)
                     /locus_tag="PK839_06695"
     CDS             complement(13304..13723)
                     /locus_tag="PK839_06695"
                     /inference="COORDINATES: protein motif:HMM:NF013411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HOG72457.1"
                     /translation="MPVVYLTRRERFSSAHRLFNPDLSDEQNLQIFGQCSHPNWHGHN
                     YELFVTVKGEVNADLGYAVNAKDISRIVHDMVVLKLDHKNINMDVDFMRGKMASTENL
                     AVSIWNELQKPLAVVGALLHCVRVVETENNSVEYYGE"
     gene            13892..14554
                     /gene="mqnB"
                     /locus_tag="PK839_06700"
     CDS             13892..14554
                     /gene="mqnB"
                     /locus_tag="PK839_06700"
                     /EC_number="3.2.2.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR03664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="futalosine hydrolase"
                     /protein_id="HOG72458.1"
                     /translation="MNRILLVLATQVEAMPIATKKLPIGFPTAMSLTERHHVDILVTG
                     AGAVPTAYHLARNLSTYNLIINLGIAGSYRAEIMPGSLVTLGSDAFADYGIDNNGHFE
                     PINSFDFNNDTPYRLDRLESPWLNQFNLALPVVKGITVGTTSGSTERIARLREQWNPD
                     IETMESAAVFFACLAAGKPFICLRAISNAVEPRNPKNWRIKLAVDRLADCTLDLLENK
                     IA"
     gene            14557..15396
                     /locus_tag="PK839_06705"
     CDS             14557..15396
                     /locus_tag="PK839_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-6-naphthoate synthase"
                     /protein_id="HOG72459.1"
                     /translation="MQSKKPLKLNFSTCPNDTFMFCAMVNRLVETKGYQFEPYLADIE
                     ELNRLAAQQLPDITKLSYGAFPELSASYQLLDSGSALGSGVGPLVVSRRKIYPDEVAY
                     TRAALPGEHTTAHLLFNLAYPNAMNKKFYLFSEIEQAVLDNEADVGVLIHESRFTYMT
                     KGLTKVADLGEFWEKRTNLPVPLGGIAIRKDLPENLKTDIAIILQSSIKFAFANRSAT
                     MPYVRRFAQEMDEEVMEKHINLYVNNFSLSLGNSGRTAVLELLKTSGKLPDNCETKSL
                     FIY"
     gene            15521..15739
                     /locus_tag="PK839_06710"
     CDS             15521..15739
                     /locus_tag="PK839_06710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72460.1"
                     /translation="MESHHHTNNNTKTPFFGKTQCIVDRIQSLDQKWYVEFWLIRYYF
                     MGIEVYRYKKEKVKLNGKNNELAKNGSE"
     gene            15980..16633
                     /gene="nth"
                     /locus_tag="PK839_06715"
     CDS             15980..16633
                     /gene="nth"
                     /locus_tag="PK839_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018360129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOG72461.1"
                     /translation="MSTNERYNRFIEYFNRTMPSATTELSYSNPYELLVAVILSAQCT
                     DKRVNMVTPDFIKRFPNPETLAASSTEEVYELVKSCTYPNSKAKHLVEMAQTLLSKFD
                     GKVPEDVQLLQQLPGVGRKTANVVASVAFNIPAMPVDTHVFRVARRLGLVTNAKTPLA
                     VERQLTEKIPAQLLATAHHWLILHGRYVCTARNPKCSECGLADICKEFRSKAKSSEK"
     gene            17076..19160
                     /locus_tag="PK839_06720"
     CDS             17076..19160
                     /locus_tag="PK839_06720"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOG72462.1"
                     /translation="MRRILYFALAIGIVLIFIPQKGRFKYEFQKGKPWMHDDLYAPFD
                     FPIYKTDAELFSERAEILKNFSPFYRFDSTVAQEAIQQFRVDFHETWVKFIAENNGVQ
                     PLSLSKVNELSTFYENEAINAIQHVMDSGILNKTENLVSNDMASSKLTILAGNVARET
                     LYENVFTKRKALEYVKNKLQQATPPGRTPVGNIWAYMDLQKYIQPNLMYDDFITQKYK
                     NEQIASISLTKGMIQVGERIISKGELITSSIYQVLESLKKEYEIHLGSRNSAIVTAGN
                     ALMVTALFIILYMFILSFRPEVFRNDAKILFILMLITLMAVISIYIIKSSLFSIYIVP
                     LVIVPIYILTFFDSRLALFTHLVTIFLVGFFVPNSFEFVFINFIAGMVAIISMTNFYR
                     RGRLFVTVGMVYATYALLLVGLAAVEEGNIKDFESINLLWFAINALLLLALYQLVYLF
                     ERIFGFLSDATLIELSDTNLDLLRKLAEVAPGTFQHSLQVANLAETAIQHVGGNPLLV
                     RAGALYHDIGKMANPLYFIENQPYDFNPHRNLDFTESASIIINHVTEGVRIGRKAGLP
                     DQLIDFIQTHHGTSKVRYFYQRHLDKFDNGAADAVKFTYPGPIPPSKETAILMMADAV
                     EAASRSMKNVTEENIKELVDTLINQQLADGQFNDANITFKDISTIKSVFKKKLLNIYH
                     ARIEYPETKANE"
     gene            complement(19249..19659)
                     /locus_tag="PK839_06725"
     CDS             complement(19249..19659)
                     /locus_tag="PK839_06725"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008628108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HOG72463.1"
                     /translation="MDKLAELFELEMEVRDYECDLEGIVNNAVYLNYLEHARHKYLLS
                     WGIDFARLHTEGIDLVVTRIEIDYKRSLASGDKFKVRVSARREGHLRLVFDQEVIAIP
                     DGSTAVKAKVIGVGLKQGRPVKIEGIPGFENFSA"
     gene            complement(19730..20518)
                     /locus_tag="PK839_06730"
     CDS             complement(19730..20518)
                     /locus_tag="PK839_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HOG72464.1"
                     /translation="MTNNPTDTQVIQDCTILSIRNLNVHAGNHHILKDINLEIPKNRI
                     TVLLGPSGCGKTTLLKCMNKLTDLYRELKVTGNIFIEDDDILNTSKNIPSIRQKMGLL
                     SQRPYPLPVSIYKNVAYGIKLKGIRDRKLIDYNVEKHLKEVGLWNEVKDRLHSPATSL
                     SIGQQQRLCLARGLAVKPRIILADEPTSALDPISSKIIENLFRNLKENYTIILVTHVL
                     RQAIRLADHVIFMYNGEIIEQGTPDELFNNPKSEKLKEYMVEGN"
     gene            complement(20543..21397)
                     /gene="pstA"
                     /locus_tag="PK839_06735"
     CDS             complement(20543..21397)
                     /gene="pstA"
                     /locus_tag="PK839_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006283819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HOG72465.1"
                     /translation="MNRWKIIEEKIFIALSAIATYSLIAVLGLIIVIVFVKGFNALSL
                     DMVIKTPKGGYYYGGEGGVLNAIVGSIYIAFGATLIAIVLGMPAALFLNVHLVRHKKT
                     QETIRYLLDVLWGIPSIVYGAFGFTLMLFFGLNASLLAGIITVALLVTPIMVRTFDEV
                     LSTIPRGLHEASLALGSTKAETSYKVMLKQGFSGFITAALLAFGRGIGDAASVLFTAG
                     YTDLIPVNLDEPAATLPLAIFFQLGSPIPEVRERAYAASTILTIIILIISLSARFLSN
                     RYSKTNIK"
     gene            complement(21394..22278)
                     /gene="pstC"
                     /locus_tag="PK839_06740"
     CDS             complement(21394..22278)
                     /gene="pstC"
                     /locus_tag="PK839_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HOG72466.1"
                     /translation="MFRRIFFDRTTKTWMITTMVIILLMPVIIGVGLYLKSLPLLEHQ
                     SLRTLLFTSDWQPSQGKFGFWPFVAGSIYVTLIAFVLSAPVCLLAGIYLTQFASSRLV
                     KVMYPVIDILAGLPSVIYGVWGILVVVPFISKFLAPIFGTTSSGYTIMAGGIVLAVMC
                     VPYMLNMITEVFKTVPMGLREASLSLGATFWESVKHVVIRKSYPGIISAFGLGIAKAF
                     GETMAVMMVVGNRVQVHFNPFEAGYPLPALIANNYGEMLSIPLYDSALMFAALLLLVI
                     ILLINLIFRYFIFKTRER"
     gene            complement(22285..23223)
                     /locus_tag="PK839_06745"
     CDS             complement(22285..23223)
                     /locus_tag="PK839_06745"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOG72467.1"
                     /translation="MKKSGIIYLLAVSLLLSACASNKQKQRTITISGAFALYPLVVTW
                     AEKYKEENPRVRFNISGGGAGKGMADALAGAVDLGMVSRSIAQEEISRGAWCVGLTID
                     AVVPTISVRNPHLNQILTRGLTREEFSAIFLKESITSWNKLLGIPGNDGITVYCRADA
                     CGAAETWANYLGGKQENIKGVGIFGDPGLAEAVANDPLGIGFNNTIFAFDIKTGKKRS
                     GVEVVPIDVNGNGQLDSTENFYTSLDLILKAISDGSYPSPPARELYFVSRGKPEKKAT
                     INFIRWCLTEGQKYVREAGYVPLNHEKINQYLKLLE"
     gene            complement(23320..24603)
                     /locus_tag="PK839_06750"
     CDS             complement(23320..24603)
                     /locus_tag="PK839_06750"
                     /inference="COORDINATES: protein motif:HMM:NF024765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HOG72468.1"
                     /translation="MSRLFKILLPLFFTIVFTPRLYAQFRIDAQLRTRFELRNGYQML
                     AAKGEDPAALVSQRTRIIFSYETDNLKLRITPQDVRLWGGDKNVNSTGITANTELGLF
                     EGFAEVKLGSVGWMSVGRQQLVYDSRHLLGNRNWNQNGISYDALLFKLALSGWNLHVA
                     GSWNTLNETSSNNYYPDSRIKSYNFVWVNHKFPGNFNLSLLHIASGVTETDSTNTLHF
                     RQTTGFYIQYPGEDLKFWANAYYQYGRNKLGKTVSAFLMDADMCYSIGKLTPGIGFGY
                     LSGNSHVGAGQSTDHLFDILYGNRHTFFGFMDYFRSFDKDTRQGGLADYYLYMDFKFT
                     KSVSLCNTAHYFMLAQSNTTTPDDRKLGYENDLILTCKFADWGGFECGYLFMLPAKSL
                     KEIQDVSNSKFSQFLYLQLTITPVIYKQQNESRSL"
     gene            24728..25417
                     /locus_tag="PK839_06755"
     CDS             24728..25417
                     /locus_tag="PK839_06755"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOG72469.1"
                     /translation="MHPNINKNEFKWLRRISMKFINRRDFIRIERSSVSLAFKKGETI
                     LKQGEQPSHVVYLERGIVKFSYQNEAGKNLILTIVAAPKILGGANLFYKDNNLFSFVA
                     VDDCDVVLIDAQVLLSVMENNAKFSIMLFQLASGMFKKSILNFISLAYKQKEGRIADI
                     IIYLSAEVYNSRDFTLTLTRRELAEFAGCSTENVIMTLSKWQNENVIAAEGKQLQIID
                     MERLKHISKIA"
     gene            25420..25827
                     /locus_tag="PK839_06760"
     CDS             25420..25827
                     /locus_tag="PK839_06760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOG72470.1"
                     /translation="MLTQKTRYSMLAMIRLAREYGKGTLLINQIAESERIPKRFLEAI
                     LLELKNNGYLGSKLGKNGGYYLLKKPEEISLLEIIRLFEGSISLLQCTSERFYQPCEH
                     CKDENSCPLRTVFGDIRSYTFNRLDETTLDKLL"
     gene            26057..27289
                     /locus_tag="PK839_06765"
     CDS             26057..27289
                     /locus_tag="PK839_06765"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ectonucleotide
                     pyrophosphatase/phosphodiesterase"
                     /protein_id="HOG72471.1"
                     /translation="MKKKLKVILLTVSCMAIALGISASNPQNVKGHPEEYVLLISFDG
                     FRWDYPTMYHTPNLDLIGIEGVHAKSLISCYPSKTFPNHYSLATGLYPDHHGIVNNSF
                     FDKKLGYYSIGNRKSVENGEFYQGEPIWVTAEKQGIRTASYYWVGTEAPIQGIQPTYW
                     KPYNERITFEQRVDTVLHWLSLPEGKRPHLITFYYHEPDLVSHNFGPSSPQTKKVVEQ
                     LDSLLGYFMLKLKLLPIYPKINVIVVSDHGMAEISSDRYINLSEHLKKEWFEYISGGN
                     PVYSLQPKIEHYAQVMDSLKKILHLNVWERGQIPSRYSYGTNPRVNDILIEAEAGWSV
                     GWSSKADDYNGGTHGYDNMLPDMQGIFYAIGPAFKHGYSCASFMNVDVYPLIAHILEL
                     NPAKTDGKLKEVKNMLRQ"
BASE COUNT         8019 a         6486 c         5848 g         6990 t
ORIGIN      
        1 tttaagcccc gaaggggcta gagagcctag acccgggcaa agccctgggt tttaaggtgg
       61 ccacgggtat gttggcgatg caagtaggag cctctctaaa gaaccattga gaccttttgc
      121 atcgcaacag gaaaaaataa ggtttatatc acaccccttc aggaattcag ggaaaaattg
      181 cacgaagtga aatcaatacc aacaatactc ctctgcccgt taaatctcag agcaaaaaac
      241 ttactcctta atgaatgtac cctggtttat ctcctcaaat gcaacgctga gctcatcgtt
      301 ggtgttcatc actatggggc ctcgccatgc tatgggttca tttatgggtt gaccaccaaa
      361 taagagaaag ttggcaccat tactttcagc tttaagctgc agaatagatc ccttgtcgag
      421 tataacacca tttaagctgc caataggctt agagtaatcg gcgaacgaga tttgcccatc
      481 gtaaacaaac aggaagtagc tggagttggc attcagcgct tcgtcaaaaa gggcatcggg
      541 ccatagccta acatcaagaa catcaatgct gctgttgtag ggtgtactta atccaacggc
      601 cttacggaat cgaccggcaa ttaccctaac cctcgatgag cttgtttcaa cctccggtat
      661 atcggatgat tttaaatcgt ggtaggtagg aggtaccatt ttaaagctac ggggaagatt
      721 cagccaaagc tgaaaaccat acattaaccc cgaaatgggc ttaggcatct cctgatggat
      781 tattccgcta cctgcagtca tccactgtac accgcccggg ttaacttcac ccctattgcc
      841 aaggctgtcg gaatgctcga cctttccctg tagcatatag gtgatggttt caattccacg
      901 atgggggtgc catggaaatc cagcagcata gtcggtagga ttttcggaac caaagtaatc
      961 cagcaagagg aagggatcag tctccattct tgtgtcatac ccgaaaaccc tatttaactt
     1021 tactcctgca ccatcggtaa cctgctgggc gggaataact ctccttaccg taatcaccac
     1081 ctgcttattt tagaccgtta agttagtaaa tattttgata atgtcaaatt aaaactctta
     1141 tggcaagttt ttaacgtgca tattgtagaa ccaggagcgc ttttacttac tatcagacta
     1201 ttttttacaa taacacttac acccaatgct ttgttgacaa aaaaatgaag agccagtagg
     1261 cagtttttcc atttcttact acttttatcg gaaattaaga tttgatggta tgacaaaact
     1321 aagggcgttt atactttctt taacgctagc tgctgccttt agccttgctc acgggcagga
     1381 aacaagcata agcacccgtg aggaagctct ttttgcaaaa gcctttgagc tttaccagaa
     1441 ggggttgtat gccagctcgc agaaaacatt ccagaagttt ctgtctgaat attcctcgga
     1501 cgttgtggcc cagctaagga ccgatgcaga attttacagc gccatttgtg ccattgaact
     1561 taacaacaac gatgctgagt accttcttgg ccgcttcatt caaaatcatc ccgaaagcca
     1621 gtacattgat ttagcctact ttgtcatggg caagcggcag taccagcaaa agaactttac
     1681 cagctcaatc tactggctat ccatgattga cggggaagga ctagacaggg aaaaacgcta
     1741 cgagatgcag ttcatgctgg gttactgcta ctttatgacg aacgattttg agctggcaaa
     1801 caagtggtta taccaggtaa aggacatgga cagcaaattt tccgggcctg caacctacta
     1861 ctactcacac attgcctacc ttcaaaaaaa atatgcaacc gcactaaagg gttttgaaaa
     1921 gcttcagaac gatgaaacgt ttgctcctat tgttcccttt tacatatccc aaatctacta
     1981 cttgcagggc gattaccaaa aggtagtggc ttatgctcct ccactggtag ccgataatgc
     2041 caccagacga acccctgaaa tagcacgaat agttggtgat tcatacttca aacttcggca
     2101 gtacgacagc gcgctagttt acctgaacaa gtatgttgaa aaaactcccc agctatcgcg
     2161 cgacgattac tacctggtag gatttaccta ctataagaca gggaattttt ctgaagcagc
     2221 caagcacctt gagcgagttt ccacggttga tgactcgtta agccaaaatg ccaactacca
     2281 cctggccgac tgctacctga agctaaacga caagcctaaa gccaggcaag cttttggaat
     2341 ggctgcaaag ctagatttcg actcttttat caaggaggat gcacttttta actttgccaa
     2401 gctcacctac gagcagcttt acgcaccctt caacgaggca attgaagcat tcaggcagta
     2461 catcaccctt tacccaaaca ctccacgtac tgacgaagcc tataactact taaccttggc
     2521 ctacctaagc acaagaaact acaaggaggc ggtagatgcg ctcgaaaaaa tcaaagtgca
     2581 agacgtaacc attaaaaccg cataccaaaa ggcaacattc tacagaggat tggagctatt
     2641 ccaaaacatg caattcgacg atgccattac gatgtttgca aaatcgctcg aaaattcagc
     2701 ctataaccct acaattgcag cgctggcaac ctactggcag gccgaggctt attatagact
     2761 agagcagttt gaaaaagcag ctgagggcta caaccgattc attttaactt ccggtgcctt
     2821 cagcttaccc gagtacctaa tggcacacta caaccttggt tattccaact tcaaacttaa
     2881 acgttacgat gaggccgttg tgtggtttcg caagtttacc aaccttagca acgatgagaa
     2941 aaacgttttt gtcggcgatg cctacaacag aatcggagat tcatttttca ttcagcgtcg
     3001 ctactgggct gccatagact actacgataa agccatggct attggcacaa ttgatgccga
     3061 ttatgccctg ttccaaagag gcttcgctct tgggctggtc gaaaggcccg aaaaaaagat
     3121 tgaatccctt cagaagctgc taaccttata ccccagctca agctacgctg acgatgcaat
     3181 ttttgaaatt gcacaaagcc agctggttct tacacaaaca ggcaaggcta tggaaaacta
     3241 taagctgatt gagaaaaact atgccggtag cagctactac gtaagggcat tggttcaact
     3301 tgggctactc tactacaatt ccgaaaaccc tgatagcgcc atgatgtact tcaagcgcgt
     3361 ggtggaagga ttcccaaatt ctcctgaagc aaaaaatgcc ctgatgggaa taagaaatat
     3421 ttacgtggac aggggcgatg caaacaccta cttcaactac gcctccagct tgggcagcct
     3481 ggccaacatg agcctagccg aaaaggactc gttaacctac atttcggccg aaaaaatcta
     3541 tataacgggc gattgtgaaa aatcaaatgc cgctttttcg agctacctgc gcgatttccc
     3601 ccagggcaac ttcagcatta acgccctttt ttaccagggt gagtgttacc gcaagcaagg
     3661 catgcgcgat agggcaattg aggctttcga tgatgttgct acccggcaaa aaaacgactt
     3721 caccggtcag agcctactca ggttaggcga actttatttt gaaaagggaa gctacgacaa
     3781 aagctatgag gcatacgcaa agcttgagtc ggttgcggag tacaaatcaa tgcttcagga
     3841 tgccaggata ggaatgctgc gctgtgcctt tgaacaaaag caatatgccg acattccggc
     3901 aatagccagc agcatacttt cggcagaaaa gctacccgat gagcttgagc gtgaaaccca
     3961 attcaagctg gctaaatcgc tctacgaact tggaagagcc gacgaggcct tagttgagta
     4021 ttcaaaggtg gctaccaatc ttaaaacagc cgagggggca gaggcaaagt acatgaagac
     4081 cagcatttac ttccagaaaa aggattacaa gaaaaccgaa gcggaggttt tcgattttgc
     4141 cgaaaagaac accccccacc agtactggct ggccaaaagc ttcatactgc tggccgaagt
     4201 gtacgccaac caggatgact tctttcaggc caaagctacc cttaatagcg ttattgacgg
     4261 gtattccagc acaaccgatg gcattattga cgaggctacc gctaagctta accagctggt
     4321 aaagaccgag aaggataagc agtcagtaaa ggttgatgag ggtgttactt tttagatgtt
     4381 ccattctttt aaactagcca gacatgcaga gcagaaaata caccaataag cacacacctc
     4441 atgttgaggt ttgccggttg gcagcgccat accatttagc caaaaaacca acaatattga
     4501 atacccataa gcacataaaa caacattttg cgcccaatat actgctggca ggcacactgg
     4561 cgtttatatt taccacaaac ctgctggttg gtaataaggc tgtagcccag gaaaccagcc
     4621 ttaaaaagga ggtaatggtt gttagaccat acgagccctc catttctgat gccttcaaaa
     4681 taaaccagct ccctaaaata gaggacacta ttagggttac cccaaacttt acctactcca
     4741 tcgttcagcg gcctgccgtt acagctttta cacctagccc tatctcagca gctcgaatga
     4801 tggccgaacc attgaaggat ttatcaaagg ggtacattaa gctggggatg ggaaacgcag
     4861 tacttccagt tgccgaggta tacttcatga gcaagcgtaa tccggacttt atgtatggcg
     4921 cttgggtaca gtaccgctca aagattggga aggttaaact agaaaacagc aaaaaggttg
     4981 atgccagctc atccacggct gatatacaga tttttgggaa aagaatgttt gccaataaaa
     5041 ttcttcaggg agatttaggc tatagcaaca acgcaagaac ctactacggg tacaacatac
     5101 tggatagcct gactagccca acatccgata agcaaagggt taacagggta aacgctaacc
     5161 tcaacttcaa cacatcccgg aaggattcag cccatttcaa ctataacgcc ctagctagct
     5221 ttgaacatct aggcgataag ttcgatatgc aggaaaacag aattaccgga ttaggaggac
     5281 tcaataagta tttaaagcag gagcaacttg gcgttgatat ctcatttacc cactactccc
     5341 gaagcagcca gtttgacatg cagaaaaatt ctattctgaa catttcacca tggattcatt
     5401 tctttgggaa gcagtggcgg gcagtagccg ggctatacat ggtttacgat gccaactccg
     5461 gaatcaacaa caccttcatc tatccacgcg ccatgctctc ctatgatgtt gtaagccact
     5521 atgttatacc ctacattgaa tttggaggct accttgagga aaactcctac tccaagctta
     5581 ttcgggagaa tccctggata acgcctggca ttagcgcctg gaacacaacc cataagctgg
     5641 tgatgacggg tggagtaaag gggaagttta gcgccaaggt atcctacaat gttcaggcct
     5701 cctactccat agttgacagc ttatacttct atacaaacat cagcatcaat ccggcaaatc
     5761 cgctgtataa taggttcaac ctggatttcg ataacgttga gcttactaaa attttaggcg
     5821 agctcaccat cgctccatcg gaaaggttca gcgttctgat tcacaccgag tacttcagct
     5881 acaaaatgaa tgagctggca catccatggc ataaacccga ctacaacacc tatgctgagc
     5941 tacgctataa cctaagggat aagatataca ccagcataca actttttgcc gtaggcaaac
     6001 gttgggtgct ctcgcccgac ggggtatcgc ctgttaggct taaagggtac gtggatgcca
     6061 acctgggatt cgactacaac ttcaacaaca ggctttatgc attcctaaac ctgaacaaca
     6121 tcacctcgtc acgctaccag gaatggtatc tctatcctgt tcagggattc aaccttcagg
     6181 ctgggctatc gtataaattc tagccggaaa tgttgaaagt ttaagatatc aggctcaact
     6241 ttgacctttc cacagcattt gcaccatttt ttcgtggaac aaatcgtatc ctttcaattc
     6301 gtaaccccct ttaaccaaac ataggaagct gctcgaaatt gcacctgagg caatttactg
     6361 gctacacaat tatttttctt caagttataa taggccaacc cgcaggattt taaaaaaatg
     6421 gactttatgg ttaaataatg ctaaatttgt ttcttgaaat tatttaactg ccgaacaatg
     6481 cactgccaaa cagcaaaaga gattaaatca agcttgataa cacacgggtt aggttggatt
     6541 tttttaattt caaccttcat gctgagcatg cctcggctag ctgcccaacc cgcaggattt
     6601 aaggaaatat ttaccgatgc cgaatactac cttctgatgg acgattacca ggaagcgcta
     6661 cccctttacc agaaacttta caagctggat tctaccaaca gcaatatcca ttaccggctt
     6721 ggccagtgct acctgaatat ttccgggaaa aaacaaatgg ccataccctt cctagaaatg
     6781 gccgctcaaa gcattagccc aaagtacgta gaagggtcat accgtgaaac aaaagctccg
     6841 ttgaatgccc tcttttacct tgggcaggcc tacatgatca acatggagct ttccaaagcc
     6901 ctcaatgcgt ttacccaatt ccgggagaac ctgaacgttg gcgatatata caacctcgac
     6961 tatgtaaacc aacagattaa ggcctgccaa accgccgcct cttttctcga aaaaccgctc
     7021 aacataaaaa aggagcggtt tgtcctgctt ccaggcagaa atgttagcat tagctatccg
     7081 gttttctcgg gcgataggca aagtttagta tttactgtaa aggaaaaatt ctacaccgct
     7141 ctataccata gcgtgaaaac caatggtgaa tggggaacac ccagcaacat cacccttgac
     7201 ttgggcgtag aaggtgaggt ttactccaca tcccttaacc atgatggcac caaactcctt
     7261 atttttatta atgacatggg ttcagggaat atctacatat ctacccttga aggtggaaag
     7321 tggcaacttg caaagaaatt tcagggtgat ataaactcta gagactggga aacctttgcc
     7381 tcctactcgg ccgatgacaa gtatattctt tttacttcga accgtaaagg tggcgttgga
     7441 gggcttgata tatacatttc cgagttactt cctggtgata aagtttcgcc tccgtccaac
     7501 cttggtgaaa tcataaatac accctacaac gaggaatcgc ccatattaag ccccgatggc
     7561 aatacccttt acttcgcatc acagggacat aacagcatgg gcggatacga tatcttctac
     7621 tccaaacgta ttcccaacgg gcaatggtca aatcctgtaa atatcggata ccccataaac
     7681 actcccgacg atgagtattt ttactttcct tttgattcca gcaccgccgt tatgccctta
     7741 gcctccaacg gtgaatcgcc atacaacgaa ctttaccaaa tctccataaa tcctgcccca
     7801 ccggtaaggt acgttggact cgacggctcc atctacctta ccgacaactc cgacatccaa
     7861 tcggctgatg ttaaaattat cattacctcc gacagtatac tgaacataag agataccatt
     7921 gttccctcca aaattggcaa gtttacatgt acactaacac cagggacata cagagttgaa
     7981 gcagccagca acttctacag catggagccg gtgcacctgt atattcctcg caactttaac
     8041 caggaggttt atccgctaga gctgaaactt caacccaaaa tggttgccga gggcgccgtc
     8101 atcaagctgc agaatattct tttcgacttc gacagctacg cccttacccg cgaagctaag
     8161 tttgaacttg aaaaggtttt caacctgatg gccgaaaatc catcattata cattgaggtt
     8221 acagggcata ccgatagtaa gggctcgccc atttacaacc taaaactttc ggccaagcgc
     8281 gccagtgccg tagtggatta cctggttgca aaagggattg acgaaagacg tttcattgtt
     8341 cgcgggctag gatcgctgga aaatcttgcc acaaacacca atcccgatgg aagtgataac
     8401 ccggccggga gacaattcaa ccgtcgtgcc agcattagaa ttttcaaccc cgataaaaaa
     8461 atcagcattg agtatgttga tgttcctgaa catcttaaac ccataaactc tcgatatacc
     8521 atactactag cccaacccgg tgttaagcct acagaaaaac agatttcaga ggtggagaaa
     8581 gccctggaca tgaatactca ggtctatgcg gttggttcct acagctttat atgcattggc
     8641 agcttctctt ccaaatccga tgccatcgaa tcgttcggga aggttgttga cctcaacttc
     8701 ccacaagcgg taattatcaa cgaaaatgag ctgcagcgaa ttgtcagatc ctcgcaaaag
     8761 cagctaatca ccaaacgcac tatctacacc atactggtgg taacctgcga aaagccgcta
     8821 aaccaagact cattcaaagg gttcccggta acggaggaga aaggcgaaga cggtctgtac
     8881 aggtactatt ttggcatctt tgacaccagg gactctgcca aaaagaatct cgacaccgtt
     8941 aactctctgg gatttccgaa tgcaattata gtgaagctcg aaaagaacag gtgatgactg
     9001 aaggtgtttt ggaaaataac atggtaaaac tcagggcacc ggaacccgat gatgtggatt
     9061 tgctctacgt ttgggaaaac aacatggaaa tatggaaggt gagcaacacc attaccccat
     9121 tctcacgcca cctgctaaaa aaatacatcg aaacagcaca cctcgacatt tgggagtcaa
     9181 aacaacttcg actcatcatt gaggctaagg atcagaacgc cctgatgaat gttcctgtag
     9241 ggttgattga cctttttgac ttcgacccct accatcttag ggcagggata ggtattctga
     9301 ttgcaaacca gcagtatcgt cgcagaggct atgctactga ggcactaaaa ctccttgttc
     9361 ggtactgttt tggcatcctc cagctaaacc agatttactg caacatatcg gccgataacc
     9421 aggttagcct gcagctcttc aagaagcaag gattcgttga ggttggggta aaaaaatcat
     9481 ggttaaaaac gcaaagcggc tgggaggatg aggtaatgct ccagctcctt aaccccaaac
     9541 cacgcctacc aaaaaactaa cccggaataa aagctgccag ggttcaaacc acaagctgca
     9601 cgagtaaaaa attccgagtt gcaattaatc aaagttaaaa aggctcggaa tatctggcca
     9661 tagagactta acctctggat ttttgctaca gaactcgttg gtgtgcttac agtaggtgta
     9721 gtcattcttc cacgatgtgc cgtttgctga aatttcccga agcgcattgg caatgtactc
     9781 cacctcggca tcggtggttg ttggatgtag cgaccaccta acccagccgg gcttctgaga
     9841 aaaatcgcca tgattaatca tttcggtaat ccggtgcgat ttatctttag taacattcaa
     9901 caggaaatgg ccgtatgtac cggcacaaac acacccgccc cgcatttgaa tgccgtagcg
     9961 ctcgcttagc agggtaacca ccaggttgta gtggagctca tcaatgtaga atgaaataga
    10021 gccaattctt tggcagcagt tttcggccaa aatatgaagc ccggggatac ccttcagctt
    10081 ttcgaaggca agattcacga gctcctcctc gcgaagggcg atatttttaa cccccatttt
    10141 ctccttgagc ctgatggcca gtgcagagcg aatggcctga aggaaaccgg gcgttccacc
    10201 atcctcccgc ctttcgatat cgtcaatata cttgtactca ccccaggggt tcgtccagtc
    10261 caccgttccg cctccagggt tatcgggaac cctgttttta taaagttgcg agttgaaaat
    10321 tagcacgccc gaagccccgg gcccaccaag aaacttgtgg ggtgaaaaga atatggcatc
    10381 cagcctctca agtggttcgg aggggtgcat gttaatttct tcatagggag cagatgcggc
    10441 ataatcaaca aaacaaaggc ctccgacctc gtgcatgata cgcgccaact cgtgaacagg
    10501 agttcggata ccggttacat tgctgcaagc cgtaaaggaa ccaatcttca ttggcctgtc
    10561 gctgtaattc tcaagtgttc ttcttaaatt agctggtgaa accatgaggt tctcgtcggg
    10621 ctccaccacc accacctcgg caatagtttc gtaccatgag gtttggttgg agtggtgctc
    10681 catgtgggta acaaacacca ccggcctctc tttagggttg gcaaagacaa agctgtagtt
    10741 ttttaacccc aggatgcgct ggaatttatt tactgaggat gtcattccga aacccgaagt
    10801 tacaattaca tcatccgaac cagcatttac gtgccgtttt atctccttta gggcaaagtg
    10861 gtaggcctcg gtcataaatt tcccagtgta gctgctctcg gtatgggtgt tggccaccat
    10921 ggggccaaaa acattcagca tcctctcctc tattggccgg tatagcctgc cactggcaat
    10981 ccagtcggca taaattaatg gcttctgccc aaaaacagtg gggtaggttg tatcaatccc
    11041 tacaatgttc ttcctgaact gctcaaagta cttctcggtt tggctcattt catagtaata
    11101 ttttgagaaa aattatcttc aactttgctc catcacgctg taatcaggaa tttcatttag
    11161 actgtaggag ttccccgatt cccagcttac agcaaaggtg tagtgtttaa ttccgtttgt
    11221 aatcaccagg tagggagcct taaagtatga gttgtagcga acaacctggt caacagtgct
    11281 ctcgctgagt tcaacatcag cagccttaca ctctacaacc actaacggct tcccggctct
    11341 accatagacc accacatcgg ctctatggtt taccccattc agcaaaaaag caacctccac
    11401 ccctattaat cccgtgggaa cattcttaaa atcgcaaagg tagcgaataa ggtgttgcct
    11461 cacccactcc tcaggggtta gaggtacgaa ttttttcctt acagcatcaa aaatctgtaa
    11521 gcttttacca acctccctta ctcggaagtc aaattctggg aaattgagcc tgcttatacc
    11581 ttcaacagcc atagggcaaa agtactaaat tccggttggg gtgcaaatgc caaccaatca
    11641 acctatagag tagaaatttg agataaagaa aatactgttc attttttcag atactcaagg
    11701 cttctaaata tgaacgggtt aaaccataag tgaataaaat atcagcaaat atttgctaac
    11761 caaccaaaaa tgaaattttt catttcaaac ccgctatcgg tttcaaaaaa tatttgttaa
    11821 tttgtgttac tagataacct aaaaaattaa cctaataatg aagaggctac tattcaccct
    11881 ggtcatagta atggcttatg gcatctcgaa tgcacagctg ttcaaggttt ttggacacga
    11941 ggtagggtat atttacgttg gacccaggct tggcatgaac ttttccaagt tttccgactt
    12001 taacggcact tatgaaaaaa gccggtttgg atatgagttt ggagcagtgg gggagtttgg
    12061 tttcaccact cgtttttcca ttcaaaccga gctgatgttc ctgtcgaagg gaagcaggga
    12121 tgagaatttt ggaaaaatca ggatgaacta catcggcata cccattctgg ccaaatacgg
    12181 attcagggcg ttggggctgg acaaggttta tgtgatgggc ggaacgtttg ccaacgtaag
    12241 ggttggcggc gcttgggtat atgacgatgg cactacctat gaactgggag gtggtatgaa
    12301 aaaattcgag tggggaatgg ggctgggtgc aggagcgcag tacccgttta aaactggtgt
    12361 agcagctttg gatttaaggt actcattggg gttctcagat attcattcgg atgataatat
    12421 tacgactcac tccagatcat tcgaggtaat tctcacctac aagttcgatt tggttgacct
    12481 gatgttcaag ttgaagaaga ataaaactcc ggaacctgaa ccagcaggta acgaagtgga
    12541 tagcaacaat gccaaggggc tgaagattga aaaaagaaac taacccttag ctattacctt
    12601 atttattttg tagaagcctg ccatcaggct tttattgtta aaagtgataa gctagtgcag
    12661 cctgacacca atcaagtgga taaactcttc gcgggtgtta tcgtgggtta ggaaagcacc
    12721 ggtgaatgca gaagtggtgg ttacggagtt ctgcttctga atgccccgca tctgcatgca
    12781 catgtgagca gcttcaatca ccacggccac tcccaatggg ttgagcgttt cctgaatgca
    12841 gtcgcggatt tgaacggtaa gccgttcctg aacctgaagc cttcgggcat aggcatctac
    12901 aaccctggca attttactta aaccggtgat atacccattt gggatgtagg caacatgagc
    12961 tttcccgtaa aagggtatca tgtggtgctc gcacagcgag tagagctcga tatccttcac
    13021 cagcaccatc tgccggtaat cctccttaaa cctggcgctt cgtatcatct catgtgggtc
    13081 aacattatag ccgtgcgtaa ggaactgaat ggacttagcc acccttacag gagtttgaac
    13141 caagccctcc cgcgaggcat cctcgccaat aagcttgatt atctcactgt agtggtaggc
    13201 aagtttttct gttgtctcgg cgttccaacg ctcaatccgg gagtaaccct cctcatcgtt
    13261 tatagatgcg ccctggttaa tttcttccat ttccacaata aatttattcc ccgtagtact
    13321 ctacagagtt attttctgtt tctactaccc tgacacagtg aagtaaagca ccaacaaccg
    13381 ctaaaggttt ttggagctca ttccagattg aaacagccag gttttcggtt gaggccattt
    13441 tgcccctcat aaaatcaaca tccatgttga tgttcttatg gtcaagcttc agcactacca
    13501 tgtcatgcac aatccggcta atatccttgg cattcaccgc ataccccaaa tccgcattaa
    13561 cctcgccctt aactgtaaca aatagctcgt agttatgccc atgccagttg ggatgcgagc
    13621 actgaccaaa aatttgaaga ttttgctcat cgctcaaatc cggattaaaa agacggtggg
    13681 ccgagctaaa acgctccctt cgtgttaggt aaacaacagg catataaacc actgtttagc
    13741 gaaaacaaac tacaaaacta agctcttaaa tttaaagtta tacgcttttt gtaattttga
    13801 tgagctaaaa tgaaactata cgactcatgt aggcagttta acattttagg aattttattg
    13861 ttcaaaacgg taatcaggcg aatttaagaa catgaaccga attcttctag ttctggcaac
    13921 ccaggttgag gccatgccaa tagccacaaa gaagctaccc ataggctttc caacggctat
    13981 gagccttacg gaaagacacc acgtagatat tctggttacc ggagctggtg cagtgcccac
    14041 agcatatcat ctggccagga atttatcaac ctacaacctc attataaact taggcatagc
    14101 tggtagctac cgggcagaaa ttatgccggg cagcttggtt actttaggta gcgacgcatt
    14161 tgccgactat gggatagata acaacgggca tttcgagcca atcaatagtt ttgattttaa
    14221 caatgacact ccttaccggc tcgataggct agaaagtccc tggttaaacc aatttaacct
    14281 agcgcttcct gttgtaaagg gcattactgt tggaactact agcggttcaa cagaaaggat
    14341 tgccaggttg cgagaacaat ggaatcccga tattgaaacc atggagtcgg cagctgtctt
    14401 ttttgcttgc ctcgctgccg gcaagccttt catttgcctg cgcgccattt ccaacgccgt
    14461 tgagccccga aacccaaaaa actggagaat taaactggcc gttgataggc tagccgattg
    14521 caccctcgac ctgctggaaa ataaaatagc ataatcatgc aaagcaaaaa acctcttaaa
    14581 cttaactttt ctacctgccc caacgatacc tttatgttct gcgctatggt taaccgcttg
    14641 gtagaaacca aagggtatca gtttgaaccc tacctggccg acatagagga gttgaaccgg
    14701 ctagcagcac aacagctacc ggatatcacc aagctaagct acggggcatt cccggagctt
    14761 tcggccagct accagctgct ggactccggt agcgccttgg gaagcggcgt tggccctctg
    14821 gtagttagca gacgtaaaat ttaccccgat gaggtggcct acaccagagc tgctctcccc
    14881 ggcgaacata ctacagccca cctgctattc aaccttgcct accccaatgc aatgaataaa
    14941 aaattctacc ttttttccga aatcgagcag gctgtgctgg acaacgaggc agatgtaggt
    15001 gtgctcatcc atgagtcacg atttacttac atgaccaagg ggctaactaa ggtagccgat
    15061 ttaggtgaat tctgggaaaa aagaaccaac ctacccgtac ccctcggggg aattgccatt
    15121 agaaaagact tacctgaaaa cctgaaaacc gacattgcca taatattgca aagcagtata
    15181 aagtttgcat ttgccaatcg ctctgccacc atgccctatg tcagacgctt tgcccaggaa
    15241 atggacgaag aagttatgga aaaacatatt aacctctacg tcaacaactt ctctctatca
    15301 ctgggtaact cgggcagaac tgccgtgctg gagctcctta aaacatccgg aaaactgccg
    15361 gacaactgtg aaaccaagtc tcttttcata tactgaatga tagccttggc attgcgcaag
    15421 ctgcttttcg ccaaataatt tgaattttta cagctcaaac cctttaaact ttgatttgaa
    15481 aacgatattt ttatgattac aataactttt taaatgctca atggaaagtc accatcatac
    15541 caacaataac accaaaacac ccttttttgg aaaaactcaa tgcattgttg acaggataca
    15601 atcgctcgac caaaaatggt atgtagaatt ctggctgatt cgttactact tcatgggaat
    15661 tgaggtttac aggtacaaaa aggagaaggt taagctgaat gggaaaaaca acgagcttgc
    15721 caagaatggt agcgagtaga atccacgcta aaatctaata cttacctctt aaatagccat
    15781 ccatatccca ttttttcttt tccggaagca atagatggat gatcaactca aagattattt
    15841 ataaagagcc atagtgcgac catctcaata caaccaacgt taacgggata gtaaattttg
    15901 ggtgtttctg agataaataa caagattttg ttgccctgcc ttcagtatat ttgaaaaaaa
    15961 tattgccata tacctgatga tttctacaaa cgaacgctac aaccgattta tagagtactt
    16021 taaccgaacc atgccgtcgg ccacaacgga gctgagctac agcaacccct acgagttgct
    16081 ggtagcagta atcctaagcg cccagtgcac cgacaagcgt gtgaacatgg taactcctga
    16141 ttttattaaa cgcttcccca accctgaaac gctggcagca tcaagcaccg aggaggtgta
    16201 cgagctggtg aaaagttgca cctaccccaa tagcaaggcc aagcatctgg tggaaatggc
    16261 tcaaaccctg ctctccaagt ttgacggcaa ggtgcccgag gatgttcaac tgctacagca
    16321 gctgccggga gttgggcgta aaacagcaaa cgtagtggcc tcggtagctt ttaacatacc
    16381 tgccatgcct gttgatacgc atgttttcag ggttgccaga cgtttaggtt tggttactaa
    16441 tgccaaaaca ccgctggcag tcgaacggca gcttactgaa aaaatcccgg cccaactgct
    16501 tgctacagcg caccattggc ttatactaca cggacgctat gtttgtacag cccgaaatcc
    16561 aaaatgtagc gaatgtggct tagctgacat ttgcaaagag tttcgcagca aggcaaaatc
    16621 ttcagaaaaa taaaagccag ctatacagta gtcaaccatc taagattcgg agaacatcaa
    16681 aaaaatatta tgcccaaata ccggggatgt acccatgcaa agaagtggct tcaaatggaa
    16741 ccctgaacta caggtaattt cttgcagcta ttctttatta aacaaccagg tagggtaact
    16801 tgcataaaca agcatgaata accgtgcgtt acctacgagt tcggcgaaac ctaaacatgg
    16861 gtatcctttc cgcttaaccc ccatgcctaa agggtatgaa aatcataaac ctaaaagtaa
    16921 cgtacaaatg aaaaaagcga aatgattcaa ccaaacaaaa taaaaagtag ctttcatttc
    16981 tgcatttcta tatttattgt catttttgcc ttcacatagc tagcattaat caaagtatga
    17041 gcatgcccca tgttgtcaaa aaaaatttca ggctgttgcg ccgcatatta tacttcgcat
    17101 tagccatcgg aattgtgctg atattcatac cccaaaaagg gaggttcaag tacgaatttc
    17161 aaaagggtaa accttggatg cacgatgatc tttatgctcc ctttgatttt cccatataca
    17221 aaacagatgc agagctcttt tcggagcgag ccgagatact aaaaaatttt agcccctttt
    17281 accgcttcga ctctacggtt gcacaggagg ctattcaaca gttcagggtt gactttcacg
    17341 aaacatgggt caaattcata gccgaaaata acggtgttca gcccctttcc ttatcaaaag
    17401 tcaatgagtt gtcaactttt tatgagaatg aggctatcaa cgccattcag cacgtaatgg
    17461 acagcgggat actcaataaa acagaaaatc tggttagcaa tgacatggca agctccaagc
    17521 taacaattct tgccggtaac gtagctcgcg aaactcttta cgaaaatgtt tttaccaaac
    17581 gtaaggctct ggagtatgta aaaaacaagc ttcaacaagc cactccccct ggcagaacac
    17641 ccgtaggcaa tatatgggct tacatggatt tacaaaagta catccagccc aacctgatgt
    17701 atgatgattt cataacccaa aagtacaaga acgagcaaat tgcgagcata tcgctaacca
    17761 aggggatgat acaggtaggc gagcgcatca tatcaaaggg tgaactcatc acttccagta
    17821 tataccaagt gttggaatcg cttaaaaagg agtacgaaat ccatctgggc agcagaaata
    17881 gcgctatagt tacagcgggt aatgcattga tggttaccgc attgtttatt attctctaca
    17941 tgtttatcct cagcttcaga cccgaagttt tccgaaacga cgcaaaaatc ctattcattc
    18001 tgatgctaat taccctgatg gctgtaattt cgatttacat aataaagagt agccttttca
    18061 gcatatacat agtccccctg gtaattgttc caatttatat cctcacattc ttcgactcgc
    18121 ggctagcgct attcactcat ctggtaacca tattcctggt aggatttttt gtacccaata
    18181 gcttcgagtt cgtttttatc aactttattg cgggaatggt ggccattata agcatgacga
    18241 acttctaccg cagggggcga ttgtttgtta ccgtgggcat ggtatatgct acctacgcct
    18301 tactcctcgt gggacttgca gctgttgagg agggtaacat aaaagatttt gagagcatta
    18361 accttctttg gtttgccata aacgccctac tgcttctggc gctataccag ctggtgtacc
    18421 tatttgaaag gatttttggc ttcctctccg atgcaacgct catcgagcta tccgatacca
    18481 acctcgattt acttcgaaag ctggccgaag ttgccccagg cacctttcag cactcccttc
    18541 aggtggccaa cctggctgaa acagcaattc aacatgttgg cggaaacccc ctgctggtta
    18601 gggctggagc tctataccat gatattggga aaatggccaa cccgttgtac ttcatcgaaa
    18661 accaaccata cgacttcaac ccccaccgaa acctggattt taccgaaagc gcatcaatca
    18721 ttatcaacca tgttaccgag ggtgtcagaa ttggccgcaa ggccggactt cccgaccagc
    18781 tgattgactt catacaaacc caccacggca cctcaaaggt tagatacttc taccagcgac
    18841 acctcgataa gtttgacaac ggcgctgccg atgccgtaaa gtttacctac cctggcccta
    18901 ttccgcccag caaggaaaca gccattctga tgatggctga tgcagtggaa gccgcctcaa
    18961 ggagtatgaa gaatgtgact gaggaaaaca taaaggagct tgttgacacg cttataaacc
    19021 agcagctggc cgatggtcag tttaacgatg ccaacatcac ctttaaggat atctcgacca
    19081 ttaaatcggt tttcaagaag aagctgctga acatctacca cgcccgaata gagtaccccg
    19141 agactaaggc taacgagtaa tttttttact ttcggatagt agtaggttga ttctcgcagg
    19201 cacttatggc ccacggtaac aaccataaaa gcaacataac caagcgaact aggccgaaaa
    19261 attttcaaaa cccggtatgc cctcaatctt aactgggcga ccctgtttta accctactcc
    19321 tataaccttc gcctttactg ccgttgagcc atcgggtatg gctatcacct cctgatcaaa
    19381 caccaaccgt aaatgcccct cacgacgagc tgataccctt accttaaatt tatcaccgct
    19441 ggcgagcgaa cgtttgtagt ctatctctat tcgggtaacc accaaatcta taccctcagt
    19501 gtgaaggcga gcaaagtcaa taccccagct gagcaaatat ttatgacggg catgctccag
    19561 gtagttcagg taaactgcgt tgtttacaat tccctcaagg tcgcactcgt agtcccgaac
    19621 ctccatctct agttcgaaca gctctgctaa tttgtccatg gtaagtaaca aatatttata
    19681 cggccattga ataacactct gaagagtagc gcaaatttgg taaaaaaaat cagtttccct
    19741 caaccatgta ctccttaagc ttctccgatt tagggttatt gaagagctca tcgggtgttc
    19801 cctgttcaat aatttcacca ttatacataa aaattacatg gtcggccaac cgaatggcct
    19861 gcctaagtac atgcgttacc aggataatgg tgtaattttc cttgaggttt cggaacaggt
    19921 tttcgataat tttactggaa atggggtcaa gggccgaggt tggctcatcg gccaggatta
    19981 ttcggggttt caccgccaaa cctcgcgcca aacaaagcct ttgctgctga ccaatggaga
    20041 ggctggtggc tggagagtgc agcctatcct taacctcgtt ccacaagccc acctccttaa
    20101 ggtgcttttc cacattgtag tctatcagct ttcggtcgcg tatcccctta agctttatcc
    20161 cgtaggctac attcttataa attgaaacgg gtaacgggta gggccgttgc gagagcaaac
    20221 ccattttttg cctaattgaa ggtatatttt tacttgtatt caggatgtca tcgtcctcga
    20281 taaaaatgtt gcccgtgacc ttaagctccc tgtacaggtc ggttagctta ttcatgcact
    20341 ttagcagggt ggttttgcca caacccgaag gacctagaag aactgttatc ctatttttag
    20401 gaatttccag gtttatatcc ttcagaatat gatggttacc cgcatgtacg tttaggtttc
    20461 tgatgcttaa aattgtacag tcctgaataa cttgagtatc agtagggtta ttggtcattt
    20521 taatatcaat ttctaagatg aactacttaa tgttagtttt tgaatatcgg ttcgaaagga
    20581 accttgcgct aaggcttata atcaggatga tgatggtgag aatagtgctt gcagcatagg
    20641 cacgctccct tacctcaggg attggagagc ccagctgaaa gaatatagcc aaaggcagcg
    20701 tggcggcagg ctcatcaagg ttaacaggta ttaaatcggt gtaacctgca gtaaaaagca
    20761 ccgaagcggc atcgccaatg cccctgccaa acgccagcaa tgcagcagtg atgaatccag
    20821 agaaaccctg cttcagcatc accttataag aggtttcggc cttggttgag cccaaggcca
    20881 agcttgcctc atgcaacccc cttggtatgg ttgaaagaac ctcatcgaag gtgcgcacca
    20941 taattggggt taccagaagg gcaactgtaa taattcctgc cagcaacgat gcatttaaac
    21001 caaagaaaag catcagcgta aaaccaaatg ccccgtaaac aatggatgga atgccccata
    21061 gcacatcgag caggtagcga atggtttcct gcgttttttt gtgccttacc aggtgaacgt
    21121 tcaggaaaag ggcagccggc atacccagta caatggcaat aagcgttgcg ccaaaagcaa
    21181 tgtaaattga acctacaatg gcattcagga ctccaccctc tcccccgtag tagtatcctc
    21241 ccttaggtgt tttaatcacc atatcgagcg acagggcgtt aaaccccttg acaaacacaa
    21301 tgacgatgat taggcccaaa acagcaataa gggagtaggt ggcaatcgcc gaaagagcta
    21361 taaatatttt ctcctctata atcttccacc tgttcatcgc tcgcgggttt taaagatgaa
    21421 gtaccgaaat atcaggttta tcaaaagaat gataaccagc agcagcaagg cggcaaacat
    21481 cagcgctgaa tcatacaggg gaatggaaag catttcgcca tagttattgg caatgagtgc
    21541 cggtaaagga tatccggctt caaaggggtt gaagtgcacc tgcacccggt tacccaccac
    21601 catcatcaca gccattgttt cgccaaatgc cttggctatg cccagcccaa aagctgaaat
    21661 tatccccggg tagctcttgc ggataaccac atgctttact gactcccaaa aggtggcacc
    21721 cagtgaaagg gaagcctctc tcaatcccat tggcaccgtt ttaaaaactt cggttatcat
    21781 gttcagcatg taggggacac acataactgc cagcacaata cctcccgcca taatggtgta
    21841 gcccgaggag gtggttccga aaataggagc aagaaatttt gaaatgaagg gcaccaccac
    21901 cagaataccc caaacaccgt aaatgacaga tggtagccca gccaatatat caattaccgg
    21961 gtacatcacc ttgaccagtc ttgatgaggc aaattgagta aggtaaatgc cggccagcag
    22021 gcaaactgga gctgatagta caaatgcaat cagggttaca taaatagaac ccgccacaaa
    22081 tggccagaag ccaaacttac cctgagatgg ctgccagtcg gaggtgaaaa gcagggttcg
    22141 gagggattgg tgctctaaca acggcagtga tttcaggtaa agcccaaccc caataattac
    22201 cggcataagc aggatgatta ccatcgtggt aatcatccat gttttagtgg tacggtcaaa
    22261 aaatattctt cgaaacatgg ctttttactc cagcagcttc aaatactggt ttatcttttc
    22321 atggttaagg ggtacatacc ccgcctccct aacatatttc tgaccttcgg taaggcacca
    22381 ccttataaaa ttaattgtag cttttttctc aggtttaccc ctggaaacaa agtaaagctc
    22441 gcgagcgggt ggcgaaggat aactcccatc gctaatggct tttagaatca ggtcaaggga
    22501 agtgtaaaaa ttttctgtag aatcaagctg accgttccca ttgacgtcga ttggaacaac
    22561 ctcaaccccg cttctctttt ttccggtttt tatatcgaag gcaaaaattg tgttgttaaa
    22621 gccaatacca agaggatcgt tggccacagc ttctgcaagt cctgggtcac caaaaattcc
    22681 aactccctta atattttcct gcttcccacc caggtagttt gcccaggttt cggcagcacc
    22741 acaggcatcg gcacggcagt acacagtaat gccatcgtta ccggggatgc cgagcagctt
    22801 gttccaactc gttatggatt ctttaagaaa tattgcggaa aactcctcac gcgttagccc
    22861 tcgcgtcaaa atctgattca gatggggatt tctaacgcta atggtgggca caacggcatc
    22921 aatggtcaat ccaacgcacc atgcacccct gctaatttcc tcctgagcaa ttgatcggga
    22981 taccatacca agatcaacag caccggcaag ggcatctgcc attcccttac ctgcgccacc
    23041 tccggaaata ttaaatctca ccctggggtt ttcctccttg tacttttcgg cccaggttac
    23101 caccagcggg tagagtgcaa atgctccaga aatggttata gtccgctgct tctgcttgtt
    23161 cgaggcacat gccgatagga gcaacgagac agctaggagg taaataattc ctgatttttt
    23221 catgtcagta gagtgtatct tttgcaatat aaatgtaacg ctttacaatt tagccacatt
    23281 aagttgaata tcaatatata aattccacat tcctatttgc tataaagacc tagattcgtt
    23341 ttgttgtttg taaatcaccg gagtaatagt gagctgcaag tacaggaact gggagaattt
    23401 gctgttggaa acatcctgaa tctccttcaa cgatttggcg ggcagcataa aaaggtaacc
    23461 gcactcaaac ccaccccaat cagcaaactt gcaggttaat atcaaatcat tctcataccc
    23521 caactttcgg tcatcagggg tagttgtgtt ggattgggcc agcatgaagt aatgggcagt
    23581 gttgcaaagg ctaaccgatt tagtgaactt gaaatccatg tagaggtagt agtcggccaa
    23641 tccgccttgc ctggtatcct tgtcaaaact tctgaagtag tccatgaacc caaaaaatgt
    23701 gtggcggtta ccgtagagaa tatcgaaaag gtggtcggtt gattgtccgg caccaacatg
    23761 gctgttaccc gataggtagc caaaccctat cccgggcgtc agctttccaa tcgagtagca
    23821 catatcggca tccatcaaaa aagcgcttac cgtttttccc agtttatttc taccatactg
    23881 gtagtatgca tttgcccaaa acttcaaatc ctctccggga tactggatgt agaatccggt
    23941 ggtttgcctg aaatgaaggg tattggttga atctgtttcg gttaccccag aagcaatgtg
    24001 gagcagggag aggttaaagt tgccagggaa tttatggtta acccaaacga aattgtagga
    24061 tttgatgcgt gagtcggggt agtagttgtt ggatgatgtc tcgttaagcg tgttccacga
    24121 gccggcaaca tgcaaattcc aaccggatag agccagcttg aaaaggaggg catcgtagct
    24181 gataccattt tgattccaat tcctatttcc caataaatgg cggctatcgt aaaccagctg
    24241 ctgcctaccc accgacatcc acccaacact tcccagctta acctcagcaa atccttcaaa
    24301 caagcccaac tcggtgtttg cggttattcc ggttgaattt acgtttttgt cgcctcccca
    24361 tagcctcaca tcctgcgggg taatccttaa tttcagatta tccgtttcgt agctaaaaat
    24421 gatacgggtg cgctgcgaaa ccagagccgc agggtcttcg cccttggcag ccaacatctg
    24481 gtagccgttg cgaagttcaa accgagtccg aagctgggca tctatcctaa actgggcgta
    24541 taggcggggt gtaaagacta tggtgaagaa aagaggtagt aaaattttaa acagcctact
    24601 catcggtttg taaatttagc caggcaaact taaacattaa atcaataggt ttagtgtata
    24661 tttgttctcc atatttatgc ataacagcat taaatattta atactttttc caaacagcct
    24721 ataactaatg caccctaata tcaacaaaaa tgagttcaaa tggttgcgaa ggatttcgat
    24781 gaagtttatc aaccgcaggg atttcatcag aattgaaagg tcgtcggtta gcttagcctt
    24841 caaaaaggga gaaaccatcc tgaaacaggg ggaacagcca tcccacgtag tttacctcga
    24901 aaggggcata gtgaagttta gctaccagaa tgaggcaggc aaaaacctga tacttaccat
    24961 tgttgcagca ccaaaaatac ttggtggcgc taacctattc tacaaggaca ataacctctt
    25021 ttcatttgtt gccgttgatg attgcgatgt ggtactcata gacgctcagg ttctcctgtc
    25081 ggtaatggaa aacaacgcta agttctccat tatgctgttt cagctggcct caggcatgtt
    25141 taaaaagtcc atactgaact tcattagcct ggcgtacaag caaaaggagg ggcgaatagc
    25201 cgatatcatc atatacctct cagccgaggt ttacaactcg agagatttta ccctcaccct
    25261 gacccggagg gagctggcag aatttgccgg atgctcaacg gaaaatgtca ttatgaccct
    25321 ctcgaaatgg caaaacgaaa atgtgatagc agctgagggt aagcaattac agataattga
    25381 tatggaacga ctaaagcaca tcagcaaaat tgcctagcca tgttgaccca aaaaacaaga
    25441 tactcgatgc tggccatgat aaggctggcc agggaatacg gcaaaggaac cctgttgata
    25501 aatcaaattg ccgaaagcga gagaataccc aaacgttttc ttgaagctat tctgctggag
    25561 cttaaaaata atggctacct tggaagcaag ttgggtaaaa acggcgggta ctacctgctt
    25621 aagaaaccag aagaaataag cctgctggaa atcataaggc tttttgaggg aagcatctcg
    25681 ctccttcagt gcacttccga aaggttttac cagccatgcg agcattgcaa ggacgaaaac
    25741 tcctgcccgc tgcgcacagt ttttggcgac ataagaagct acacatttaa caggttggac
    25801 gaaactaccc tcgataaact tttatgaaga actttttctc ctgtgcataa aaccaaaact
    25861 cttagaccgt gtcgaactat atcggagtta acccaccgta ttacacgtta gaaatcatac
    25921 aggttaactt tacggcaaga gggaaacttt cagggaaaca atactaatac tgttgaacaa
    25981 attatgattt atgtgtttgt actacgagtt tcaatttcag gcaaagccat ccattacctt
    26041 ataaggttgt aaagaaatga agaaaaaatt aaaagttatt ttgctgacag taagctgtat
    26101 ggctattgct ttaggcatta gcgcatcaaa tccgcagaac gtgaagggtc atcctgagga
    26161 gtatgttctg cttatctcat ttgacggttt tcggtgggac taccccacca tgtaccatac
    26221 tcccaatctc gacctgatcg gaattgaggg tgttcacgcc aaatcgttaa ttagctgcta
    26281 cccctccaaa actttcccca accactacag cctggcgacc ggcctgtacc ccgaccacca
    26341 tggaattgtg aataactcat tttttgacaa aaagcttggt tactactcca ttggtaacag
    26401 gaagagtgtg gaaaatgggg agttctatca gggtgagccc atctgggtaa ccgctgagaa
    26461 acaaggcatt aggacagcct cttactactg ggtaggaacc gaagcaccta ttcagggtat
    26521 tcaacccaca tactggaagc catacaacga aaggattacc ttcgaacagc gtgttgacac
    26581 cgttcttcac tggctatcgc ttcccgaagg aaaacgacct catctcatca ccttctacta
    26641 ccatgagccc gacctggtga gccataattt tggcccgtca tcgccccaaa ccaaaaaggt
    26701 agttgaacag ctcgacagcc tgctgggcta ctttatgctt aaacttaaat tgcttcccat
    26761 ttaccctaaa ataaacgtga ttgttgtttc ggatcacgga atggcagaaa tttcaagcga
    26821 taggtacatt aacctttcgg aacatttaaa aaaagagtgg tttgagtata tatcgggagg
    26881 taatccggtt tacagccttc aacccaaaat tgagcattac gcccaagtca tggatagcct
    26941 aaaaaagatt ttgcacctaa atgtttggga gcgaggccaa ataccctcga ggtattccta
    27001 cggaaccaac ccgcgtgtaa acgatatcct catcgaagcc gaggcaggat ggagcgtggg
    27061 atggtcgtca aaagccgatg attacaacgg gggtacacac gggtatgaca acatgctccc
    27121 cgacatgcag ggaatattct acgccattgg accggcattt aagcatggat acagctgtgc
    27181 ttcgttcatg aatgttgatg tttacccgtt gattgcccat atattagagc ttaacccggc
    27241 gaaaactgac ggtaaactta aagaagtaaa aaatatgttg agacaatagg gaaatcaaaa
    27301 aagttaaatt aagatttgag gattaagaaa tagatacccc ttg
//
LOCUS       DAOSHM010000034        26869 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_1400_length_26869_cov_23.121765, whole genome shotgun
            sequence.
ACCESSION   DAOSHM010000034 DAOSHM010000000
VERSION     DAOSHM010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 26869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26869
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1400_length_26869_cov_23.121765"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            271..645
                     /locus_tag="PK839_06770"
     CDS             271..645
                     /locus_tag="PK839_06770"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOG72472.1"
                     /translation="MNRIKNGKKFLFFIGKTGIVFPDFQLLFNRSANENLEFGLRSTG
                     WKDKRAIAEQIHHVLKQVGMETKGYKMPHQLSGGEQQHVVTTRSLLNSSEIILADEPT
                     GNLDLGRIFWNRFMPFGNRELR"
     gene            783..1388
                     /locus_tag="PK839_06775"
     CDS             783..1388
                     /locus_tag="PK839_06775"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HOG72473.1"
                     /translation="MNKKYITGFVLTGGKSSRLGVEKGLMLFDRKPLVQHVLETLSVV
                     VGEVFISANNPDYKQFSKPIIPDIVQGIGPMGGVYSCLMQSATDYNIFLSCDTPFVPS
                     DLFLYLIKEIDGHSCVVPVHDDEKTEPLCGIYSKQCADTFMEFISKGNYKMMDVLKAV
                     DYHPVRVDESLPFYNNHLFFNINTPADCKLAMQYLQNNSLK"
     gene            1412..1975
                     /locus_tag="PK839_06780"
     CDS             1412..1975
                     /locus_tag="PK839_06780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72474.1"
                     /translation="MLTIPNLFLVGGNSRHSGKTTLACEIIRRLAKEQEVTAIKFIRF
                     DPDEKEMHGSHLGESEFSGYSIMRETNNHSSKDTSQMLQAGAKEVFYIRSGEEFMEQA
                     SIDFLTRYNHGQPLVCESSALRHFVEPAVFVMMMRIPPIGNPKSVESYLPLADRILYF
                     DKSYDERLRMLEELRFDSGKFYLPSFK"
     gene            complement(1954..3672)
                     /locus_tag="PK839_06785"
     CDS             complement(1954..3672)
                     /locus_tag="PK839_06785"
                     /inference="COORDINATES: protein motif:HMM:NF015239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017111 - ribonucleoside triphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphatase"
                     /protein_id="HOG72475.1"
                     /translation="MKQSQTTLNEKWIKASILGSMWASVEIVWGSFLHNLRVPFSGHI
                     LTAIGLIILISVSYRWREKGLFWRAGVICALLKTMSPSAIILGPMIAILSEAILLEAA
                     VSLLGKNRIGFTVGAILAMSWNLVQFIISKVIAYGYNLVKLYESIMQYVQKQLNTNID
                     MVWAPIILLLTIYILLGIGTAIAGIFIGKKLGSTDLNAEIFKAKNKASLLPTSKYTRF
                     PFSTAWLIADVLSIILVLVLAKRINVILWFGISILVVVCWIFHYKVALRRLGQPKLWI
                     SFFIITVIAAIAFSQFQSGISLWQGTLIGLEMNVRAIVMVIGFAVIGTELYSPKIKNL
                     FQKKALISLYMALDLSAESLPNMIALVPNLKEIFKHPLEIVKQLLSYAEYRMHEIESR
                     KQHKAAIAIITGEKSSGKTKFLTETATTLRENGIDVGGVYTKKIRENDEITAYLIVNV
                     ATGEEYPFLQKNELESELRIGAFSIDAEGLEFGKKNIVKSFDSRVLMIDEIGKLEMSG
                     GGWHNELNLLPLEKQLYILLSIRQEFVEDVIEKWSLHPAAIYNVAECNPEILIQEITA
                     YLKEGR"
     gene            complement(3755..>4541)
                     /locus_tag="PK839_06790"
     CDS             complement(3755..>4541)
                     /locus_tag="PK839_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72476.1"
                     /translation="NTSITNNQQNYSIQMNRNEHKLHKVENAKIMNSDFTCSTGVDDK
                     CRLNDKIDFTARLPYNYLKSLEADDQVTAADTTNFPKNNHHAANAQIAMSSMPNHKLS
                     VSAFVAQKTRFTTLRERHSYKMEQELFKLNFQSVKAAHSYISFQYNLLRNLPIDGSIH
                     HIRLNDVIKSECGVQGNLIQLQNAGKSRFNGADLSLHFALLYNLDLTASYSFIIQKKN
                     SNPSCGIVTNEFHLFNLQGSASRKQFTLFAGIKNLFDANYAYA"
     assembly_gap    4542..4641
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4723..5544)
                     /locus_tag="PK839_06795"
     CDS             complement(4723..5544)
                     /locus_tag="PK839_06795"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HOG72477.1"
                     /translation="MKQHLLTLIALTIFITTIKAAETDATKISKTFQLGEFVLSAPKM
                     REKVEQTIHLQMNTTNISEALCTVSSVIFANMGGRFEPTVVVSGFDIHGIRVFVDGLS
                     LQKFYNGDTDMGQLATFDYSLVSLTKGSTPMSSGMNATGGSINLISFQPTDKLNLQVI
                     AGLASGNTYEYGVNVGSRWKMFFFQSSYYQQRFSERETSKSDILLLPINNLRTIIKIS
                     TMQREINLTVEAIKPNQHDSGNSRYRNISVYILLQTIKSTMKTASKVFTPTNYTT"
     gene            complement(5598..5939)
                     /locus_tag="PK839_06800"
     CDS             complement(5598..5939)
                     /locus_tag="PK839_06800"
                     /inference="COORDINATES: protein motif:HMM:NF012354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOG72478.1"
                     /translation="MNSNFQIEFRFWVSFDGKPLLGKGKMDLLRKIDQRGSLRQAAEE
                     LRISYRKAYYRIYSMNKAAKQPVVELKRGGKNGGVSEITTYGKKLMKEYEIIEKQLTD
                     FIQNCKFEAVH"
     gene            complement(6028..6732)
                     /locus_tag="PK839_06805"
     CDS             complement(6028..6732)
                     /locus_tag="PK839_06805"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOG72479.1"
                     /translation="MGREIIMANKNKKKHWGMVIDLTRCVGCQTCAVGCKIKNNLADG
                     MWWNRVITVGGDHVDTAGGTYNHPMRYSIPLNCQNCENPICVDVCPTGASYQREEDNL
                     VLVDYDKCIGCRACVEACPYGVRLPNHNEPQYEHYDVQLGSDEVTPRYQGVVEKCTFC
                     VERIDQGLNPFCTDVCPERARYFGDLNDEDSDVSKLIRENPVETLHPELGTKPSVYFI
                     PPSKKKTTRTGLDYIK"
     gene            complement(6717..9134)
                     /locus_tag="PK839_06810"
     CDS             complement(6717..9134)
                     /locus_tag="PK839_06810"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOG72480.1"
                     /translation="MSTEIKKFSACVPNCWMNCRLYAHVRDGKLVQTSAAPFPDPSYN
                     RICLRGLSHPQRVNSKDRILHPMKRVGKRGENKFEKITWDEAIDILADNLYKIKEKYG
                     SKAVAFIPWSGSYASINGGMPGAIKRFANIFEGTVVIDGVDFGMPTGMSQVVANMAGG
                     MASLFLGPEPKDLSYAKTIIFWGTNITDSQVHNWHFVLDALENGAKLICIDPRYSETA
                     AKSHEWVRIRPGSDSILALSMIQVIIEENLIDRDYCIQHTVAPFLVRQDNGLFLRQND
                     IDGSDNAGYLTMDADSNELKALETVTNPALLGTFDVTLHNGSKLQVKTALQMLKDEAD
                     KYKPAEAERLTLVPADKIVEIARIYATNTPSTIYTGFGVDRWDNADQVGRGIATLGVI
                     TGQIAKKGASPLGAMGQSAIQYTISDAVMGPWTAPTGTMANLLNFVLLQQAILTGKVD
                     MWEPKDPANPLAGPTGREPVSKDWPIKGLILTTGNLVSNHAEQKVTIDEMFSEDKLDF
                     ICCIDMFMTDTARQADLFLPCTSWFENDDIVGGLHPYVMRMEKAIEPMGESKGDWDIF
                     KLLAQKMGWGEYFKGDAKDQIDGILDVLGNLFGDHKDKILKEFKENGIARLSDPNALP
                     FADGAYYTRTGRAEFYSEGVATVFPKSMPALQLPVNEGHNPLPHFVPPAEAWIDNPLI
                     KKYPLALATLHTKWRVHTTFHEVPALREMDPEPVVYLNKKESSKRGLKDGDYVKVFND
                     RGHCVVRLVVDDSINDGGAAIPKGWQRHQFKEGGYQELTKNHINTVHYGMSFFDALVE
                     VTKWEGK"
     gene            complement(9156..10088)
                     /locus_tag="PK839_06815"
     CDS             complement(9156..10088)
                     /locus_tag="PK839_06815"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF015851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HOG72481.1"
                     /translation="MWTQTPSIIENEQPADRFALHYVPQTHWRIWHAIWFWAMGIGSG
                     IFCLRQFLGFSGGGLFWGMNAIDVIGLVLVAIGGIILILDLGKPFRFLNSLLKPKNAW
                     ISRGAISDFIFLGIGVLYVLPGLTPSLPWSDAVFSEADAFGRILIVLAIFFAFIIMIY
                     PGFVLYYSKNIPFWNSILTPILFLEYALTSSFGLLGLMGALNGDMLPDIFYILFTTTL
                     VLTLLTTVLYILERKSVGNPCIDESIHCLLKGKASGWFWLSQILGIVVPILLLATRSD
                     DFTVLLWSGICTIIGSFIFRYSQLIAGYRVSPLI"
     gene            10449..11279
                     /locus_tag="PK839_06820"
     CDS             10449..11279
                     /locus_tag="PK839_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005865855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HOG72482.1"
                     /translation="MKSTKVLFISQEIMPYLPETQISKIGRYLPQGIQERGKEIRTFM
                     PRFGLVNERRNQLHEVIRLSGMNLVIEDTDHPLIIKVASIQSARMQVYFIDNDDYFQR
                     KYTTFDCDGKEFPDNDERAIFFCKGVLETVKKLRWTPDLVHCHGWITAFAPILIKKTY
                     AEDPLFAKAKVIYSVYNDEFGTPLDLNVRKKILMEGITKKDVEILKEPSYVNLSKLAI
                     THADAVIVGDRQINPELQEFINTVKKPILPYQNEENYIDVYSEFYDTILNSKTNVKKK
                     "
     gene            11263..12579
                     /locus_tag="PK839_06825"
     CDS             11263..12579
                     /locus_tag="PK839_06825"
                     /inference="COORDINATES: protein motif:HMM:NF025458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4270 family protein"
                     /protein_id="HOG72483.1"
                     /translation="MLRRNRPSLILQHIHKPNPGKALIILPLLLISIQSCYNDPNFLG
                     NNLIPEEDIYAVKTDTLFEVSAYSIKTDTILATSGYLGYYNSEIFGSTKGSFVGRYLP
                     TTSTEGFGGPTAKPDSLFFYLKTSSYYGDTLISLAIRLHEITDTAVVWNPTSSLVDLE
                     GKYNPNPLASGSYRGSQVLKIAIDTTFARSLMDSSALANPKEFYTKFKGLYLTCDDLD
                     GFGGVAYYLSTSETYFRLYYHYTKVIDGKDSTISTNKAFTFNSYRFFQYLHDPSKATP
                     SLAVQHLNDTLYQDTVFYVQGMGGVYGKISLDDIAQWKDSVPVAINRAELVIGRVIPQ
                     TVTSDSSLSQLLLYYKDNGVWAGVIPDQISQTTGGVNSNGKLRLYNNDYSIVITSHLQ
                     RILNGEISDKNLYVFPNKTSTFAYSVLQTGNSSRKIKLKLTYTKIR"
     gene            12579..14414
                     /gene="glmS"
                     /locus_tag="PK839_06830"
     CDS             12579..14414
                     /gene="glmS"
                     /locus_tag="PK839_06830"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016662822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HOG72484.1"
                     /translation="MCGIIGYIGFREAYPVILNGLKRLEYRGYDSAGICVVDGEPFTI
                     KCKGKVSDLENLIGKNSVAGKVGIGHTRWATHGEPNDINAHPHESMNGKFILIHNGII
                     ENYSILKSKLEKRGYTFKSETDTEVLVNLIEYIYQKGNVTPEIAIRLALTKVVGAYGI
                     AVICCDEPETLIAARKGSPLVVGVGEGEYFVASDATPIVEYTNSVIFLNDGDVAILQR
                     DQMVLKTIFNESLEPNIQTLDLDIEAIEKNGFEHFMLKEIYEQPRSIHDTFRGRISLD
                     PIDIHLGGLYNVMPNLVNAKRILMIGCGTSWHAALVGEYVIEDLARIPVEVEYASEFR
                     YRNPIISKDDVVIAISQSGETADTLAAIKLAKEAGALVLGICNVVGSSIPRETHAGVY
                     THAGPEIGVASTKAFTAQVTVLIMMAILLGRKRGTLTEEHYQTLIQEMEKVPEKIEQI
                     LNSDSIVKSISERFKTTNNFLYLGRGYYFPVALEGALKLKEISYIHAEGYPAAEMKHG
                     PIALIDDTMPVVVIATKDNSYEKIVSNIQEVKARKGVIIAIVTEGDTVIREMADYVIE
                     IPEAVEAIGALLAVIPLQLLAYHIALLRGCNVDQPRNLAKSVTVE"
     gene            complement(14526..15581)
                     /gene="rfbB"
                     /locus_tag="PK839_06835"
     CDS             complement(14526..15581)
                     /gene="rfbB"
                     /locus_tag="PK839_06835"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HOG72485.1"
                     /translation="MKRNILITGGAGFIGSHVVRRFVKKYPDCLIVNMDKLTYAGNLM
                     NLADVENEPNYRFVKGDIADEHEVEEVFCSYEIDGVIHLAAESHVDRSIADPMSFIKT
                     NIVGTTTLLNAALKSWGHNLQNKLFYHISTDEVYGSLGSEGFFTEQTSYDPRSPYSAS
                     KASSDHLVRAYHHTYGLPTVISNCSNNYGPNQFPEKLIPLTINNIKNKKPIPVYGKGE
                     NIRDWLYVEDHAAAIDLVFHRGEHGKTYNIGGNNEWKNIDLIRLLCRILDDKLDRQKG
                     ESEKLITFVRDRAGHDLRYAIDSSKIRSELGWRPSVSFEEGLERTVDWYLENNAWLEE
                     VVTGQYLTYYEKQYANR"
     gene            15672..15744
                     /locus_tag="PK839_06840"
     tRNA            15672..15744
                     /locus_tag="PK839_06840"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15705..15707,aa:Lys,seq:ctt)
     gene            15858..16526
                     /locus_tag="PK839_06845"
     CDS             15858..16526
                     /locus_tag="PK839_06845"
                     /inference="COORDINATES: protein motif:HMM:TIGR02453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HOG72486.1"
                     /translation="MGTCIEASTLNFLADLQGNNNREWFQQNRNRYELAFSNFYQLVL
                     SLIAGIGSFDKNLGSPDPKDCIFRIYRDVRFSPNKMPYKQNFGAYIAKGGKKSNLAGY
                     YIHIEPHESFAAGGIYMAPSDIMRRVREDIYDYPEDFLSIVNSKEFRGKFSFEDAESL
                     GKVPRGFEAGTAVDEYLKLKNLIPSISFSDSSIQKETFVDEVLQVYKALQPLVGFINQ
                     AINQ"
     gene            16543..17292
                     /locus_tag="PK839_06850"
     CDS             16543..17292
                     /locus_tag="PK839_06850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HOG72487.1"
                     /translation="MRGMLAFLFTSLLIVRVGYAQVGFTQEGVASFYGDEFEGRVTAS
                     GERYSHNKATCAHLSIPFGSLVKITNLDNGQTAIVRVNDRGPFVPDRVVDVSRSVAER
                     LGMIPTGVARVKVEVVDANAPLPANQNAIEEVPAKPKVGTVKVAGSSSTSKTSTAQGE
                     GEFYQLKVTPVKPDQYGIQIGSYKEPVNVFKIASDFQKLLKQSMMINVVNVNGEKVYR
                     LLVGGFETRKEAEEVKNKIQNDYPGCFVVGF"
     gene            17395..18096
                     /locus_tag="PK839_06855"
     CDS             17395..18096
                     /locus_tag="PK839_06855"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HOG72488.1"
                     /translation="MKIGTLVNDFINLFYPNICAVCHDGLARGEEVICTQCLYHIPRT
                     RYWLETDNPVARLFWGRVNIENACSLFLFAKGSKYRKLIHQLKYNGREDIGVFLGREF
                     GKELCRAELYKSVNFIIPVPLHPRRQKERGYNQAEVIANGLSKSMGIPVIPDVLVRRV
                     YTQTQTKKSRTERVQNVSGAFDLQNPEKISKHHVLLVDDVITTGSTLEVCAQTLLRVP
                     EIKVSVGTLAYASSW"
     gene            complement(18217..19389)
                     /locus_tag="PK839_06860"
     CDS             complement(18217..19389)
                     /locus_tag="PK839_06860"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HOG72489.1"
                     /translation="MKRISITLMLLAPVIAGNAQQVTEAVSMFAFDLYAKTAAGNSSN
                     FVFSPLSITPAFGMVALGARGETQKQLDEVFHFQPTTNFHSELGKLQQKVLKSAGDSV
                     EICMANRAWLEKSYRVLGKYSKALRRSYSAKIATADFVGNFEASRLLINKTVEADTRS
                     YIKNLLPEGSLDEMTRLVLTNAIYFKGKWEVPFDPKRTSARNFNLSSGETVKCPTMLA
                     DDKFGYYVGTNYQALELNYRGNKLSLLILLPNEDTPMADFERNLSLQLYQQATKGLNP
                     EKVTVLLPKFTIETSLSLKQTLSDMGMPIAFSNAANFSGISGKDDLKISDAFHKAYIE
                     VSEEGTTAAAATAVVVAVKSMLPANTFVANRPFIYILRHKDSGAILFMGKVENPLR"
     gene            complement(19522..19827)
                     /locus_tag="PK839_06865"
     CDS             complement(19522..19827)
                     /locus_tag="PK839_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72490.1"
                     /translation="MKMIYCTCNVAVLESLLTMLDENNVRDFQVVEQVTAKNKKGNHR
                     FNNPVWPGYNSTVFIQIAEDEKAKMVMQKIREFNQKAFNDNELVTACMWTIEDYFFE"
     gene            complement(19867..22995)
                     /locus_tag="PK839_06870"
     CDS             complement(19867..22995)
                     /locus_tag="PK839_06870"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOG72491.1"
                     /translation="MKLPEVAVKRPVAITMLFIAVLLFGVVSLTRLPLDIMPDMELPT
                     LTVITVYPGASASEVEQQITKELEMILSGTENLKNISSQSKENVSFVQLQFDWGADIT
                     SAANNVRDLIELVKNKLPREAQNPTIFKVNSSLMPVLAYSIGAKESYGALNKLVNDEI
                     ASKLRKVSGVGSVIIIGEPEREIKINVNPLKLNAYNLSMSQIANILQASNLSIPGGNI
                     KIGSSDYAVQIPGDVASIDELAEMPLTSFNGKVIKLSDVADIHDEFKEKDEYPRTSSG
                     KGLIMMIQKQSGENTLNVANAIRLKVDELKKTLPADVTMSEVMKSDELVSESISNLSE
                     TLWYALFFVILVVLAFLRNWNQSFIIFITIPFSLIVAFIVMFAAGWTINIFSLMSLIV
                     VSGMVVDDAIVVLENITRHVENGEKPKQAAIFGTSEMGLAISASTFTTIVVFLPMVFM
                     GGIVGIMFKQLAILTSVTLLASLFTALTLTPMASSLLVKGIKPGEVHRHNRFFTASEK
                     LLTGLEGWYKKALAWVVYHKAITVIIVTIILGLSVWMGRNMGTDYIPEFDAGDVVAVV
                     ETEVGTAAAKTDSVANVVLKIFEEEIPEMAPGSIFSIAGQTEDGILSSVGFSEGKNVA
                     TIMCHLRKPNERSASAREIANRLRPRISEIPEIENFTLLGGSVLTTALTGNKKPIEIE
                     ISGNNFSTINQTAEALTAKLKQSDYFTNVDNDVDKGKLEVQVKIDKQKASAMGLNAAM
                     IGLQVRQSIYGTEAGEYKEDGDKFDITLRYSPSYRNDINQIRNIQLKNLLNQDIPLYA
                     VAQIETGTSPLQINRKEQQRLVKVMAELNGVSLGDGQMEAQRIVSSTSFPNDVDVKIA
                     GQTSDQGESFADLYLILILGILLVFMVMAAQFESFKDPFIIMFAIPVTFVGVVWAFKL
                     TGLTLSIVTFVGMIMLVGIVVKNGIVLVDYTNLLRKRGYSLYEAIQEAGRSRLRPVLM
                     TTLTTILGMVPMALSKGMGREAYSPLGVTMIGGLLISTLITLFLVPAIYGIFHNSERK
                     SNGVAKKIDYSR"
     gene            complement(23167..24213)
                     /locus_tag="PK839_06875"
     CDS             complement(23167..24213)
                     /locus_tag="PK839_06875"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOG72492.1"
                     /translation="MKPTILGLTPLAIALVLTGCKTSKTEVPATEENVPLVSTVVVEE
                     RIYSPTLSFTGTAFANREANLGATLPGKVEKIYFDEGQNVHEGDLIVELSDELLTQTI
                     IENETIKKDFDRVSRLREKGSISEMEYDHVKAQYDASQAKVQMVKKNTRVYAPFSGMV
                     VERMMEEGEVYFLNPGLEPGYSMRSGIVRLMQLNPVKVRFDVNEKDLGVIKKGLEVEV
                     TFDGVAGKTYQGKVGTIKQILSTTTHSAAVEVTISNPQLEIKPGMFAYITVKLPEIKA
                     SVVPLKSIYRLPGTSDDYLFSVTNDTINRIPISRLESIGEYVAVKGIEPGVEVVLDGK
                     TRVSQGQRVRVSGK"
     gene            complement(24264..25721)
                     /locus_tag="PK839_06880"
     CDS             complement(24264..25721)
                     /locus_tag="PK839_06880"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOG72493.1"
                     /translation="MKRLLYIFVLLVLADGVSNAQQLLTVEQCRQMALEHNQRMLIAG
                     EHVQAATSLKSSAKTQFFPSVSANGLYMRTNKKFSLFENDLFLPVIPYSAIDPSTGSF
                     NPNLDPANTFVINPSTLQPVTDANGNPIFQNYTWLPKDQAEVGYKNVYTAGVSLTQPI
                     FTGGKIRETYRIAKYGESLARANEAAERSDIIYKTEEAYWRVVAVGEKVKLVNASIQL
                     LEKLSVDIENFYEEGMVIQNDLLKVKVKLNEAQLNLVKVQNGLALSKMALCQQVGLPL
                     NQEILLADSLVASMDTAVIMQSFTDTALAKRPEIEMLNQTINIAKSGVNLMRSRFMPN
                     IGLSANYMLFNPNPYNGFSENFGGDWNVGVVLNIPIFHWNDRGHTLKAAHSEQRVAEL
                     KMDEAKELISLQVQQAVYQLNESVKKVQMAEENLKQAEENLKVTRNAFDEGKLKTSDV
                     LEAQTLWQNAYSDLIDARMEYRLNITNLKKVTGTL"
     gene            complement(25924..26526)
                     /locus_tag="PK839_06885"
     CDS             complement(25924..26526)
                     /locus_tag="PK839_06885"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOG72494.1"
                     /translation="MHQEVIGSPHDEMRNSIINAATKAFSRFGFRRTSMDDIARSLRM
                     GKSSIYYYFKGKEEIFRAVVAREASQQRENVKKILESPDDAVSKMRSYVKLRMEFIRH
                     LSSFVEQRLNDDLKSLQLTEKFRKKYDDEEISIITQILEEGSRSGAFKVKDFYMSALA
                     IVTAMKGLELPLVNSTNDENAMNPVIDDLLDILFFGIVKR"
BASE COUNT         7300 a         5854 c         5861 g         7754 t
ORIGIN      
        1 gctcttgtca accccaacga ccctggctat ctcaattttt ttgtacccgt ttcccaacat
       61 ttgcgaaata gtgtaccttt gttcttgcgt taagtgtccc atcctgcaac ttatttctgg
      121 ggagatttag ggggtaagga tagccaccta accgtcagga acgacaaggg agagactagc
      181 cctttcactc tcgttcttga aaggttagtt ttttcgttcg ccccttaagc taacgcccca
      241 aaaagttgca tttgtaatga aaatttatgt ttgaatagaa taaaaaacgg aaagaaattc
      301 ctattcttca taggaaaaac gggaattgtt tttccggatt ttcaattgct tttcaatcga
      361 agtgccaacg aaaacttgga atttggactt cgatccaccg gttggaaaga taaaagggct
      421 attgcagagc agattcatca tgtgttgaaa caggttggta tggaaacgaa aggttataaa
      481 atgccccatc aactttccgg tggagaacag caacatgttg ttactacacg ttcgttactc
      541 aattcatcgg aaataattct tgccgatgaa ccaaccggca atcttgattt gggaaggata
      601 ttttggaatc gctttatgcc gtttggaaat cgggaactac ggtgattgtt gctaccccta
      661 atttaagttt gattgacatg tttcctgggc gtgtttgggt atgtgaagat gaaaaattga
      721 ctgaaataga taatgaagtc aaaatcattg aaatacaaga atgagataag tccaaaccat
      781 ttgtgaataa aaaatatatt accggttttg tactaaccgg aggcaaaagt tcccgtttgg
      841 gtgtcgaaaa ggggttgatg ctatttgaca ggaagccact tgtacaacat gtattggaaa
      901 cgttgagcgt tgttgttgga gaggttttca ttagtgcaaa caatccggat tacaaacaat
      961 tttcaaaacc cattatccct gatattgttc aaggaatcgg tccgatgggt ggagtttatt
     1021 catgcctcat gcagtcggca accgactaca acatttttct atcgtgcgat acgccttttg
     1081 ttccgtctga cttgtttctg tatttgatta aggaaatcga cggtcattcc tgtgtagttc
     1141 cggtgcacga cgatgaaaaa acagaaccgc tttgtggcat ttacagcaag caatgcgcag
     1201 atacgtttat ggaatttatt agcaaaggca attacaaaat gatggacgtg ttgaaagctg
     1261 tggattatca tcccgtaaga gtggacgaat cattgccatt ttacaacaat catctttttt
     1321 tcaacatcaa cacaccggct gattgcaagt tggcgatgca atacttgcag aataattctt
     1381 tgaaataatt tgtaaagtta actgattatc aatgttaacg attcccaatc tttttcttgt
     1441 cggcggcaat tctcgccatt ctggcaaaac gactttagcg tgtgagatta ttcgtcgatt
     1501 ggcgaaggag caggaggtta ccgctatcaa attcatacga tttgatcctg atgaaaaaga
     1561 gatgcatggc agccatttgg gagaaagtga attttcgggt tattccatca tgcgagaaac
     1621 caacaatcat tcatcgaagg acacttctca aatgttgcaa gcaggagcga aagaagtgtt
     1681 ttacattcgc tcgggagaag aatttatgga gcaggcctcg attgattttt taacgagata
     1741 taatcacggt caacctttgg tgtgtgagag cagcgctttg cgccattttg tcgaaccggc
     1801 cgtttttgtc atgatgatgc gaattccgcc catcgggaat cctaaatctg tagaaagtta
     1861 tttaccacta gccgatcgaa ttttgtattt cgacaaaagt tatgacgaaa gattgcgaat
     1921 gcttgaggaa ttgcgatttg atagcggaaa gttttatctt ccttctttca agtaggctgt
     1981 aatttcttga atcagtattt ctgggttaca ttctgccaca ttgtaaattg ctgccggatg
     2041 aagactccat ttttcgatga cgtcttccac aaattcctgt cgtattgata gcaatatata
     2101 caattgtttc tcgagaggca acaaattcaa ttcgttgtgc catccgccgc cgctcatttc
     2161 caatttgccg atttcgtcaa tcatcaatac cctcgaatca aacgatttca ctatattttt
     2221 tttgccaaat tcgagtccct cagcatcaat tgaaaaagct ccaatacgca attccgactc
     2281 caattcattt ttctgcaaga aaggatattc ttcgccggtt gcaacgttta caatcagata
     2341 agctgtaatt tcatcgtttt ccctgatttt ttttgtataa acacctccta cgtcaattcc
     2401 attttctcta agcgttgttg ctgtctctgt caaaaatttg gttttgccac tgcttttttc
     2461 gccggtaatt attgctattg ctgctttatg ttgttttcgc gattctattt catgcatccg
     2521 gtattcggca tacgacaaca attgttttac gatttcgagt ggatgtttga agatctcttt
     2581 cagattggga acaagcgcaa tcatattcgg aaggctctcg gccgaaaggt caagagccat
     2641 atatagcgag atcaaggctt ttttttgaaa gagattttta attttaggac tgtaaagttc
     2701 tgtccctatt accgcaaaac cgatcaccat aactatggca cgaacattca tttccaatcc
     2761 aatgagtgtt ccttgccaca acgaaatacc cgactgaaat tgtgaaaaag caatggcggc
     2821 aatcacggtg atgataaaaa acgaaatcca caatttaggt tggcccaaac gtcgcagggc
     2881 aactttataa tggaaaatcc aacaaactac gacaagaatg ctgattccaa accaaagaat
     2941 gacattaatt cttttggcaa gtaccagcac taaaatgatt gatagaacat cggcaataag
     3001 ccatgctgtt gaaaaaggga aacgagtata ttttgaagtt ggcaaaagcg atgctttgtt
     3061 tttcgctttg aatatttcag cattcaaatc ggttgatcca agttttttcc caatgaaaat
     3121 gcctgcaata gctgttccaa tacctaaaag aatataaatg gtcaacaaca aaattatcgg
     3181 agcccaaacc atatctatat tggtattcag ctgtttttga acatattgca tgatgctttc
     3241 atacaatttt accaaattgt atccgtaagc aattactttg ctgataataa actgaaccag
     3301 attccacgac atggctaaaa tagctcctac ggtaaatccg attctgtttt ttcctaataa
     3361 gctcactgcc gcttcaagca aaattgcttc actgagaatg gcaatcatcg gccctagtat
     3421 aatagcactt ggcgacatcg ttttcagcaa agcacagatg acgcctgccc gccaaaacaa
     3481 acccttttct ctccagcgat aacttacaga aataaggata atcaacccaa tggctgtgag
     3541 aatatgcccg ctgaatggaa ctctcagatt atgcagaaaa ctgccccata caatttccac
     3601 cgaagcccac atcgagccaa gtatggatgc tttaatccat ttttcgttca gggttgtttg
     3661 cgattgtttc acaagattga attttgaaaa cggtttttta atttagatga aatagaagtt
     3721 tggtaaagta atttcttcca atttcctgat aaccttaggc gtaagcgtaa ttcgcatcga
     3781 aaaggttttt aatgccagca aaaagtgtga actgtttcct cgaagccgaa ccttgtaagt
     3841 tgaacagatg aaactcattg gtaacaatgc cacaggaagg atttgaattt tttttttgaa
     3901 tgataaagga atatgatgcc gtcaaatcga gattatacag caatgcaaaa tgcaagctga
     3961 ggtctgcacc attaaaacgc gatttccctg cattctgaag ctggatgagg tttccctgaa
     4021 cgccacactc cgatttaatg acatcattca gccgaatatg gtgtattgaa ccatcgatgg
     4081 gaagattcct taaaagattg tattgaaacg aaatgtagga atgagcggct tttacagact
     4141 gaaaattcag cttgaaaagt tcttgttcca ttttgtacga atgtctttcc cttagggttg
     4201 taaaacgtgt tttttgagca acaaatgccg aaacggataa tttgtgattc ggcatcgaac
     4261 tcatagctat ctgtgcattt gcagcatgat gattattttt cgggaaatta gtcgtatcgg
     4321 cagctgtaac ctgatcatca gcttcgaggc tttttagata gttgtagggc aatcgggcag
     4381 tgaagtcgat tttatcgttg agtcggcatt tatcgtccac tcctgtgctg caagtaaaat
     4441 cgctgttcat aatcttcgca ttttccactt tgtgcaattt gtgctcgttt cggttcattt
     4501 gaatcgaata attttgctga ttgttcgtta tcgaagtatt tnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4621 nnnnnnnnnn nnnnnnnnnn ncaaaattcc gatggttgtt catgcttgta aacgcatatt
     4681 ttttggtttg acttgtatgg gtttaatcat caaagctttt cattacgttg tataatttgt
     4741 cggcgtaaag acttttgatg ctgttttcat tgttgatttt attgtttgca ataaaatata
     4801 aacgctgatg ttccgatacc ggctattgcc agaatcgtgt tgattgggtt ttattgcttc
     4861 cactgttagg ttgatttccc tttgcattgt actgattttg ataattgtgc gtaaattatt
     4921 gatcgggagc agcaatatat ccgattttga agtttccctt tctgaaaatc gttgttgata
     4981 ataactcgat tgaaagaaaa acattttcca tcggcttcct acgttcactc catattcgta
     5041 ggtgtttccg cttgccaatc cagcaattac ctgtaaattt agcttatcag ttggctgaaa
     5101 acttatcaaa ttgatgcttc cgccggtagc attcataccc gacgacatgg gggtggaacc
     5161 cttggttaat gaaacaagcg aataatcgaa cgtagcgagt tgtcccatat cggtgtcccc
     5221 gttgtaaaac ttttgtagtg aaagcccgtc aacaaaaacc cgaatgccgt gaatatcgaa
     5281 tccgctaact actaccgtag gttcaaaacg tccgcccata ttggcaaaaa tgacggatga
     5341 aacagtacac aatgcttcgc ttatgttcgt ggtgttcatt tgcaaatgaa ttgtttgctc
     5401 taccttttct ctcatttttg gggctgataa aacgaattcg cccaattgga aggtttttga
     5461 tattttggta gcgtcggttt ctgccgcttt tatggtagtg atgaatatcg tgagagcaat
     5521 caatgttaag agatgctgtt tcataagatg ttctgtattg tcgttattcc taaaatgaat
     5581 aacgggatgt aaaaaattta gtgtacagct tcaaatttgc aattttgaat aaagtcggtc
     5641 aattgttttt ctattatctc gtattctttc atgagttttt ttccataagt tgttatttct
     5701 gacactcccc cattctttcc acctcttttc aactccacaa caggttgttt ggcggcttta
     5761 ttcatcgaat aaattcgata ataagccttc cgatacgaga tgcgcaactc ttcggctgcc
     5821 tgtcgtagcg atccgcgctg gtcaatctta cgaagcaaat ccattttacc tttcccgagc
     5881 aaaggttttc cgtcgaatga aacccaaaat ctgaattcta tctgaaaatt cgagttcaac
     5941 ttttcaaaat tattttgttt agggaaataa aaaggtaaac acaagacttt tctttacctt
     6001 gtgtttacct ttttcagcaa aatgatctta tttgatataa tccaatcccg ttcgggtggt
     6061 ttttttctta cttggaggaa tgaaataaac gctcggtttt gttcccaatt cgggatgaag
     6121 tgtttcaacc ggattttcgc gaatgagttt cgagacatca ctatcctcat cgtttaaatc
     6181 gccaaaataa cgagcacgtt caggacaaac atcagtgcag aaaggattca aaccctgatc
     6241 gatacgttcc acacaaaatg tacatttttc cactacgccc tgatatctcg gcgtaacctc
     6301 atccgaaccg agttgcacat cgtagtgctc atattgcggc tcattatggt ttggcaggcg
     6361 tactccatat ggacatgctt ccacacaggc acggcatccg atacatttgt cgtaatccac
     6421 cagaaccaaa ttgtcctctt ctctttggta ggatgctccg gtcggacaaa catccacaca
     6481 aatcggattt tcgcaattct gacaattcaa cggaatggaa taacgcatcg gatgattgta
     6541 ggttcctccg gcagtatcca catgatcgcc tcctacggtg ataactctgt tccaccacat
     6601 gccatcagcc aaattatttt ttatcttgca tccgactgcg catgtttgac aacccacaca
     6661 gcgggtcagg tcaatcacca ttccccaatg ttttttcttg tttttatttg ccataattat
     6721 ttcccttccc attttgttac ttctaccaag gcatcgaaaa acgacatgcc gtagtgaact
     6781 gtattgatat gatttttagt taattcctga tatccacctt ctttgaattg atgacgctgc
     6841 caacctttgg gaattgcagc acctccatcg ttgatggaat cgtccaccac gagtcgtaca
     6901 acgcaatgac cacggtcgtt aaatacttta acgtaatcgc catctttaag gcctctttta
     6961 ctcgattcct ttttattgag atagaccacc ggttcgggat ccatttcccg taacgccgga
     7021 acctcatgaa aagtagtatg cacacgccat tttgtatgta gcgtagctaa ggcgagcgga
     7081 tattttttta tcagcggatt atcaatccat gcttctgccg gtggcacaaa atgaggcaat
     7141 ggattatgcc cttcgttgac aggcagctgc aatgctggca tacttttggg gaatacagtt
     7201 gccacacctt ccgaataaaa ttcggccctg cctgttcgcg tatagtaagc gccatcggcg
     7261 aatggaagtg cattcggatc ggagagccgc gctattccat tttccttaaa ctctttcaat
     7321 atcttatctt tatgatctcc aaaaagattg cccaatacat cgaggattcc gtcgatttga
     7381 tcttttgcgt ctcccttgaa atattctccc caacccattt tttgagctaa cagcttgaag
     7441 atatcccaat cgcctttact ttcgcccatt ggttcaatgg ctttctccat ccgcataaca
     7501 taaggatgaa gtcctcctac aatatcgtcg ttttcgaacc agctggtaca aggcaggaac
     7561 aaatcggcct gtcgagccgt atcggtcatg aacatatcaa tgcagcaaat aaaatcgagt
     7621 ttatcttcgc tgaacatttc atcgatggta actttctgct cggcatgatt gctcaccaga
     7681 ttgccggtag tcaaaatcaa acctttaatc ggccaatctt tcgaaacggg ttctctgcct
     7741 gtaggccctg caagaggatt ggcaggatct ttgggctccc acatatccac cttccctgta
     7801 agaatagctt gttgcagcaa tacgaagttc aacaaattgg ccattgttcc tgttggagca
     7861 gtccatggtc ccatcacagc atcggaaata gtatattgaa tggcactttg acccattgcc
     7921 ccgagaggag aagctccttt tttggcaatt tggccagtga taactcctaa tgttgcaatg
     7981 cctctaccca cctgatcggc attatcccaa cgatcaactc caaatccggt atagatggtt
     8041 gaaggcgtat tggttgcata aattcgagca atttctacaa tcttatcagc agggacaagt
     8101 gtcaaccttt cggcttcagc cggtttgtat ttgtccgctt catccttcaa catttgcaag
     8161 gctgttttta cctgcaattt tgatccgttg tgtagcgtaa cgtcgaaagt ccccaataag
     8221 gcaggatttg taaccgtttc caatgcttta agttcattcg aatcggcatc cattgtcagg
     8281 tatccggcat tatcgctgcc gtcgatatcg ttttgacgca ggaaaagtcc attatcctga
     8341 cgaacaagga atggggcaac ggtgtgttga atgcaataat ctcggtcgat gaggttttct
     8401 tcgatgatta cctgaatcat ggataaagct aaaatggaat ccgatcctgg acgaatgcga
     8461 acccattcgt gcgatttggc tgccgtttcg ctgtaacggg gatcaatgca aatcagtttt
     8521 gcaccgttct ccaatgcatc gagcacgaaa tgccagttat gcacttgcga gtcggtaatg
     8581 tttgttcccc aaaagatgat ggttttggca tagctcaaat ctttgggttc aggtcctaaa
     8641 aagagcgaag ccattccacc ggccatgttg gcaactactt gcgacatgcc ggttggcatt
     8701 ccgaaatcga caccatctat tacaaccgta ccttcaaaaa tgttggcaaa gcgtttaatg
     8761 gcgcctggca tacctccatt gatagaagca taactaccgg accagggaat aaacgcaact
     8821 gcttttgaac catacttttc tttgatttta tataaattgt cagccaaaat atcgatggct
     8881 tcgtcccacg tgattttttc gaatttgttt tcgccacgtt tccctacacg tttcatcggg
     8941 tgaaggatac ggtctttgct gttgacccgt tgtgggtgcg ataatcctcg caggcaaata
     9001 cgattgtagg aaggatcggg aaaaggagca gccgatgtct gaaccagttt tccgtctcga
     9061 acgtgagcat ataatcggca gttcatccaa cagttgggaa cgcatgccga gaattttttt
     9121 atttctgtac tcattccagc tattttttcc aattattaaa ttaaaggtga aacgcgataa
     9181 ccggcaatca attgactgta gcggaatatg aaacttccaa taatagtgca gatgccgctc
     9241 caaaggagta ctgtaaaatc atcactcctt gtggctaata acagaatggg aacaacaatt
     9301 cctagtattt gcgaaagcca aaaccatccg gaggctttac ctttcaacaa acaatgaatg
     9361 gattcatcaa tgcatggatt cccgacactt ttgcgttcta gaatataaag aacagttgtt
     9421 aacaacgtta acacaagagt tgtagtaaat aaaatgtaga aaatatcggg aagcatatct
     9481 ccattcaaag ctcccatcaa tcccagcaat ccaaagctgc tcgtaagagc atattctaaa
     9541 aataaaattg gcgtcaaaat gctattccag aagggaatgt ttttagaata ataaagcaca
     9601 aatccgggat aaatcataat aatgaaagca aagaaaatgg ccagaacgat gagaatccga
     9661 ccgaaagcat cggcttctga gaatactgca tccgaccaag gtagcgaagg agttagtcct
     9721 ggaaggacgt aaagaacacc aattccaaga aaaataaagt ccgaaatcgc tcctcgcgaa
     9781 atccatgcat ttttaggttt tagtaatgaa tttaaaaatc gaaaaggctt acctaaatca
     9841 aggatcagaa taattccacc aatagccact aaaaccaatc cgatgacatc aatggcattc
     9901 atcccccaaa atagtcctcc tccgctgaac cctaaaaact gccttaaaca aaaaatgcca
     9961 cttccgatgc ccattgccca aaaccaaatg gcatgccaaa tgcgccaatg ggtttgagga
    10021 acatagtgaa gcgcaaatcg gtcagcaggt tgttcgtttt caatgatact gggtgtttgt
    10081 gtccacatag ttagaattta ttttgttgtt aattgatatc tgattgaaat taaaactgac
    10141 actcctttta catctgaatt aattcatcaa atgtataaat atatttatga ataatcataa
    10201 atttgataag gaaaattctt aatatcatgt aaatttaaat tagaaaaaac ctgcaaagaa
    10261 agtcggtttt tttttatatt tttgtaagat tttattgttg agaagcccgg tttcggactt
    10321 agtagctgaa gccgggtttt accagtattc ctaaggtagg tgcaagattc agtgtaagag
    10381 atctatgatg cataggatta tttgcatcac cagcagggga ggtgaagtta aacttaaaag
    10441 taaaaaccgt gaaaagcacc aaggtactgt ttatctcgca ggaaatcatg ccctacctgc
    10501 ctgaaaccca gatatccaaa ataggtcgct atttgcctca gggtattcag gagcggggaa
    10561 aggagatacg aacctttatg ccaaggtttg ggctggtaaa cgaacgacgg aaccagctgc
    10621 acgaggttat ccggctttcc gggatgaacc tagtaattga ggataccgat catcccctga
    10681 taatcaaggt tgcctctatt cagagcgcac gcatgcaggt ttacttcatt gacaatgacg
    10741 actactttca gcgaaaatat accaccttcg actgtgatgg taaggagttt cctgacaacg
    10801 acgaaagggc aattttcttc tgtaagggtg tactcgaaac ggttaaaaag ttgcgctgga
    10861 ctcccgattt ggttcattgc catggctgga taactgcatt tgcacccatt ctcataaaaa
    10921 aaacctatgc cgaagacccc ctgtttgcca aggcaaaagt catttattca gtttacaacg
    10981 acgagtttgg cacgccgctt gatttgaatg tgcgtaagaa aattttgatg gagggcatta
    11041 ccaaaaagga tgtggaaatt ttaaaagagc catcctatgt taacctgagt aagctggcaa
    11101 ttacccatgc ggatgccgtt attgtcggcg ataggcaaat taaccccgag ttacaggaat
    11161 ttataaatac tgttaaaaag cccatcctgc cttaccaaaa tgaggaaaat tatattgatg
    11221 tttacagcga attttatgat accatcctta atagcaaaac aaatgttaag aagaaataga
    11281 cctagtttaa tattgcaaca tatccataag ccaaaccccg gcaaagcgct tatcatttta
    11341 cccttgcttt taatatccat tcaatcgtgc tacaacgatc ccaattttct gggcaacaac
    11401 cttatacccg aagaggatat ttacgctgta aaaaccgata cgctatttga agtttctgcc
    11461 tactcaataa agacagacac cattcttgcc acaagcggtt atttaggcta ctacaatagc
    11521 gaaatttttg gctccaccaa ggggagcttt gtgggtagat acctgccaac aacctccacc
    11581 gagggttttg gagggccaac cgccaagcca gattcccttt tcttctacct gaagacctca
    11641 tcgtactatg gtgatactct cattagtctg gcaatcaggt tacacgaaat taccgatacg
    11701 gcggttgttt ggaaccccac cagcagcctg gtggaccttg agggaaagta taacccgaac
    11761 cccttggcaa gcggctcata caggggaagt caagttttaa aaattgcaat tgataccact
    11821 ttcgcgcgca gcctgatgga ctcatcggca ctggctaacc caaaggaatt ttataccaaa
    11881 ttcaagggtc tttatctaac ctgcgacgat cttgatggtt ttggaggcgt tgcctactac
    11941 ctttcaacat ccgaaaccta tttcaggctt tactatcatt acacaaaggt tatagatggt
    12001 aaggattcta ccattagcac caacaaggcc tttacattca actcataccg ttttttccag
    12061 taccttcacg atccctcaaa agcaacccca tcgttagccg ttcagcattt aaacgacacg
    12121 ctttaccagg acaccgtgtt ctatgttcag ggtatgggcg gggtgtatgg aaaaattagc
    12181 cttgacgata ttgcacaatg gaaggattca gtacccgttg ctataaatag ggctgagctg
    12241 gttattgggc gggtaattcc tcaaacggtt acatcggact catcgttaag ccaactcctc
    12301 ctttactata aggataatgg ggtatgggca ggtgtaatcc ccgaccagat tagccaaact
    12361 actggcgggg taaacagcaa tgggaagctg aggctttaca acaacgacta cagcattgta
    12421 attacctccc atcttcagag aatccttaac ggggaaatct ccgataaaaa tctgtacgta
    12481 ttccccaaca agaccagcac ttttgcctac tctgtgctcc aaacagggaa cagcagccga
    12541 aaaatcaagt tgaagttaac ctatacaaaa ataaggtaat gtgtggtata attggctata
    12601 ttgggtttag ggaggcctat cccgttatac ttaacggact taagcgtttg gagtatcggg
    12661 gctacgattc ggccggtata tgtgtggttg atggtgaacc gttcactata aagtgcaagg
    12721 gaaaggtttc cgacttggaa aacctcattg ggaagaattc tgttgctgga aaagttggaa
    12781 tagggcacac ccggtgggca acccatggtg agccaaacga tattaacgct cacccacatg
    12841 agtcgatgaa cggaaagttc atccttatcc ataacgggat aatagagaac tacagcatac
    12901 ttaagagcaa gctggaaaag cggggctaca cttttaaatc ggaaaccgac actgaggtgc
    12961 tggtcaacct tattgagtac atctaccaga agggtaatgt aacccctgaa attgctattc
    13021 gcttggcatt aacaaaggtt gtaggtgctt atggaatagc ggtaatttgc tgcgatgaac
    13081 ccgaaactct cattgctgca cgtaagggaa gtcctcttgt tgttggcgtt ggcgagggtg
    13141 agtactttgt tgcttccgat gccactccaa ttgtggagta caccaacagc gtaatttttc
    13201 tcaacgacgg ggatgtggct attttacaac gagaccagat ggtgctaaaa accatattta
    13261 acgaatcgct ggagcccaac attcaaaccc ttgacctcga tattgaggcc atagaaaaaa
    13321 atggcttcga gcatttcatg ctaaaggaga tatacgagca accgcgtagc attcatgata
    13381 cctttagagg gagaatctct ctggacccta ttgatattca ccttggagga ttgtacaacg
    13441 taatgcccaa cctggttaat gctaagcgca ttctgatgat aggttgcggg acatcgtggc
    13501 acgccgcact ggtaggcgaa tacgttattg aagatttagc tagaattccc gttgaggtgg
    13561 agtatgcctc ggagtttaga taccgaaacc ccatcatcag caaggacgat gtggtgattg
    13621 ccatcagcca gagcggcgaa actgccgata ccctagctgc cattaaactt gctaaggaag
    13681 caggagcctt ggtgctgggt atttgcaacg tggtgggttc cagcataccc cgcgaaaccc
    13741 atgccggcgt ttacacccat gccggccccg aaataggggt tgcctctact aaagctttca
    13801 ccgctcaggt taccgttcta attatgatgg ccattttgct gggacgcaaa aggggaacac
    13861 ttaccgagga gcactaccag acattaattc aggaaatgga gaaggtcccc gaaaaaattg
    13921 agcagatact taactccgat tcaattgtta aaagcatttc cgagagattt aagacaacca
    13981 ataacttcct gtaccttggc agaggctact acttccccgt ggcccttgag ggagcgctta
    14041 agctgaagga gatatcctac attcatgctg aggggtatcc agctgcagag atgaagcatg
    14101 ggccaatagc gctaattgac gatacgatgc ccgttgtggt tattgccact aaggacaaca
    14161 gctatgaaaa gattgtgagc aacatccagg aggttaaagc tcgaaagggc gtaatcatcg
    14221 caatagttac cgaaggagac acggtaatca gagaaatggc cgattacgtg atcgaaattc
    14281 ccgaagccgt tgaggcaatt ggcgccctgt tggctgttat tccattgcag ctgctagcct
    14341 accacattgc cctgcttagg ggttgcaatg ttgaccagcc acgcaacctg gccaaatcgg
    14401 ttacggtgga gtagaatttt cagaaaaata ctccagaaat tgtttcaggc acattttaaa
    14461 attaaaaagg agatggcttt ctatctcctt ttttatgttt gtatgtttag tcaagcacta
    14521 aagggctacc tgttggcata ctgtttctcg taataggtta agtactggcc tgtaacaact
    14581 tcctctagcc aggcgttgtt ttccagatac caatccacgg ttcgctcaag cccctcctca
    14641 aagctaacgg atggtcgcca tccaagctcg ctacggatct tgctggagtc tattgcatac
    14701 ctcaagtcgt gtccagccct atccctgaca aaggtaatca gcttttcaga ctctcctttt
    14761 tgcctgtcca gcttatcatc caaaatccgg caaagcaatc ggatgagatc aatattcttc
    14821 cactcgttgt ttcctccaat attatacgtt ttaccgtgtt cgcccctatg aaaaacgagg
    14881 tcaatggcag cggcatggtc ttcaacgtaa agccagtcgc gaatattttc acctttcccg
    14941 taaaccggaa tgggtttttt gtttttaatg ttgtttattg tgagcggaat caacttctcc
    15001 gggaactggt tgggaccgta gttattggag cagtttgaaa taacggttgg caacccgtag
    15061 gtatggtggt aggctcgtac caggtggtcg gaagaggcct tcgaggcgga gtaagggcta
    15121 cgtggatcgt atgatgtttg ctcggtaaag aatccttcgc tgcctaagct gccgtaaacc
    15181 tcatcggttg aaatgtggta gaacagctta ttttgtaggt tgtgtcccca gctcttcagg
    15241 gcagcattca aaagggttgt agttccaaca atgttggttt taatgaacga catggggtct
    15301 gctatggagc ggtcaacatg cgactcggct gccaggtgta ttacgccatc aatctcgtag
    15361 ctacaaaaaa cttcctcaac ctcgtgctca tcggctatat cacccttcac aaaacggtag
    15421 ttaggctcgt tttcaacatc ggcgaggttc atcaggttac cggcataggt tagcttatcc
    15481 atgttcacaa tcaggcagtc gggatacttc tttacaaaac gcctaactac atgtgaacct
    15541 atgaaacctg cgcctccggt aatcagaata tttctcttca ttacgttaaa atttcaggta
    15601 aagtaagcaa taataatttt caattttttg gataaggtga aaaataccta taaatttgcc
    15661 accacaaaaa tcggttcgta gctcagctgg tagagcacgt gactcttaat catggggtcg
    15721 agggttcgag cccctccgaa ccgacagcgc caccttaagg tggctttttg ttttaaacca
    15781 tttccaccaa ccaattgcag ggagctccat tcgctacagt ttgcaaattt tattaatttt
    15841 ggtaaaaatt atgctgtatg ggtacctgca tcgaagcttc aaccttaaac ttcttggctg
    15901 atttacaagg aaataacaat cgtgagtggt ttcagcaaaa tcgaaaccgt tacgaattgg
    15961 ccttcagtaa tttttaccag ctggtgctgt cgttgattgc cggtattggc tcttttgata
    16021 aaaacttggg atcgcccgat cctaaggatt gcattttcag aatataccgc gacgtacgtt
    16081 tttcacctaa caagatgccc tacaagcaaa attttggggc atatattgcc aaaggaggga
    16141 aaaagagtaa cctggccggc tactatattc atattgagcc acacgaatca tttgccgccg
    16201 gtggaatata catggcgcct tccgacatca tgcggagagt tagggaggat atttatgatt
    16261 acccggaaga ttttttgtca attgtaaatt caaaggagtt tagagggaaa ttttcctttg
    16321 aggatgcgga aagccttgga aaagttccca gaggttttga agccggaaca gcagttgacg
    16381 agtacttaaa gcttaaaaac cttataccct ccatttcatt cagcgatagc agcatacaga
    16441 aggagacttt tgtggacgag gtgctgcagg tatataaagc gctgcagcca cttgtgggtt
    16501 tcataaatca agccataaac cagtaaagta taaaatatga gtatgagggg aatgcttgct
    16561 tttcttttta cctcactttt aatagtaagg gttggatatg cccaggttgg cttcacccag
    16621 gagggagttg catcttttta cggtgatgag tttgaaggca gggtaaccgc ttcgggcgaa
    16681 aggtacagcc acaataaggc tacctgtgcg cacctgtcca ttccgtttgg ctcgctggtg
    16741 aaaattacca atttggataa tggacaaaca gcaattgtta gggttaacga cagggggcct
    16801 tttgtgcccg acagggtggt tgacgttagc cgttcggttg ccgaaaggct gggtatgatt
    16861 ccaaccggtg tagcccgggt taaagttgaa gtggttgatg caaatgcgcc cctgccagcc
    16921 aaccagaatg cgattgagga agtacccgca aaacctaagg ttggtactgt taaggtggca
    16981 ggctcatcca gcacgtccaa aacttccacg gctcaaggcg agggggagtt ttaccaactt
    17041 aaggttacac cagtgaagcc tgaccaatat ggcattcaaa ttggcagcta caaggagccg
    17101 gttaacgttt ttaagattgc aagcgatttc cagaaattgc tgaagcagag catgatgatt
    17161 aatgtggtta atgttaatgg cgaaaaggtt taccgcctgc tagtaggtgg attcgaaacc
    17221 cggaaggagg ccgaggaggt aaagaataaa attcaaaacg attacccggg ctgctttgtg
    17281 gttgggtttt agcattaaaa attgcggtgg gtagtaagta attagggtat tcggcatttt
    17341 aagagttaat ttcctacctc aggagtaggt tgggttattg caaagcttaa gggaatgaaa
    17401 ataggtacgt tggtaaacga ttttataaac ctgttttacc cgaatatctg cgccgtgtgc
    17461 catgacgggt tggcaagggg tgaggaagtt atctgcactc aatgtttata ccatattccc
    17521 agaacaagat actggctgga aaccgataat cctgtggcgc ggctattctg ggggagggta
    17581 aatattgaaa atgcctgttc gctattccta ttcgcaaagg ggagtaagta ccgaaagctt
    17641 attcatcagc taaaatacaa cgggagggaa gacatcggtg tttttctagg cagggagttt
    17701 ggaaaggagc tctgcagggc agagctttat aaatcggtaa atttcataat acccgtaccg
    17761 cttcatccca ggaggcagaa ggaaaggggc tataaccagg cagaggtgat tgccaacggg
    17821 ctttcaaagt caatgggaat accggttatt ccggatgtgc tggtgcgaag ggtttacacc
    17881 caaacccaga ccaaaaagtc gagaaccgaa agagttcaga acgtttcggg agcttttgac
    17941 cttcagaatc ctgaaaagat ttcgaagcat cacgttttgc tggttgacga tgtaattaca
    18001 acagggtcaa cgctggaggt gtgcgctcag acacttctac gagttcctga aatcaaggtg
    18061 agcgttggaa cactagccta cgcttcgtca tggtaaacta aatatagggt aatttgaaaa
    18121 atgcaggggt aaaaatcgcc ctgcatactt catacagcca attcaaccgg caacagtaac
    18181 ttttgcggct caactggccg cttggaggtt gaaattctac ctaagtggat tttccacctt
    18241 gcccataaag agaatggcac cggaatcctt atggcgaagg atgtaaataa aaggcctgtt
    18301 agcaacaaag gtgttggccg gtagcatcga tttaacggct actacaacag cagtggcagc
    18361 agcagcagtt gtaccctcct ctgaaacctc gatgtaagcc ttgtgaaacg catcggaaat
    18421 cttcaggtca tccttgcctg aaattcccga aaagttggca gcattgctaa aggctatggg
    18481 catacccatg tcgctaagcg tttgctttag gctgagggag gtttcaatgg tgaacttagg
    18541 caggagcacg gtaacttttt cagggttcag cccctttgta gcctgttggt agagttgtag
    18601 tgaaagattt ctctcaaaat cggccatggg tgtatcctcg ttgggcagaa ggattagcag
    18661 cgaaagtttg ttcccgcggt agtttagctc cagtgcctgg tagttggttc ccacgtagta
    18721 tccaaacttg tcatcggcaa gcatggtagg acatttcacc gtttcacccg atgaaaggtt
    18781 gaagtttctt gccgaagttc gtttgggatc gaacggtact tcccatttac ccttgaagta
    18841 gatagcattg gttagcacca ggcgggtcat ctcgtcgagg ctaccttccg ggagaagatt
    18901 tttaatgtag ctccttgtgt cggcttcaac agttttgttg atcagcaacc gtgaggcttc
    18961 aaaatttcca acaaaatcgg cagtggcaat tttagcgctg tatgagcgtc gtagcgcctt
    19021 gctgtacttg ccaagcaccc tgtatgattt ttcgagccag gcgcggttgg ccatgcaaat
    19081 ttccaccgag tcgccggccg attttaaaac cttttgctgg agtttaccca actcggaatg
    19141 aaagttggtt gttggctgga agtggaaaac ctcatccagc tgtttttggg tttcgcccct
    19201 tgcgcctagc gcaaccattc caaaagcagg agtaatgctt aggggagaaa aaacaaagtt
    19261 gctgctgttc ccggcagctg tttttgcata aaggtcgaag gcaaacattg agacagcctc
    19321 agtaacctgc tgggcattac cggctattac cggggccagc aacatcaaag taatggatat
    19381 ccgcttcatg gtagtaattt tttaccaagt tacaacaaaa gttatgcaag tcataagcct
    19441 gccttgcagg aattgttttt tctctgtgct tcccgcatgt tcgccgtaga agtgccaaag
    19501 ttaatggatt gctgtatgct tttactcgaa aaagtaatcc tcaatagtcc acatgcaggc
    19561 tgtaaccagc tcgttatcgt taaatgcttt ttggttgaac tcccgtattt tttgcatcac
    19621 catttttgcc ttttcatcct ctgcaatctg gataaaaact gtagagttat agccaggcca
    19681 aacggggttg ttgaaccggt ggttaccctt tttgtttttt gccgtaacct gttccaccac
    19741 ctggaagtcg cgcacgttgt tttcgtccag catggttagt agcgattcca aaacggcaac
    19801 gttacaggtg cagtaaatca ttttcatgtg ttgcagctat tagtgaaatt aacatgaagc
    19861 tataaactat ctggaatagt caattttttt agctactcca tttgattttc tttctgaatt
    19921 gtggaaaatt ccatagatag ccggaacaag gaacagggta atcagcgttg aaatcagcag
    19981 accgccaatc atggtaacgc caaggggtga gtaagcctcg cgccccatac ccttgctcaa
    20041 ggccatgggc accattccaa ggattgtggt taaggtagtc atcagcacgg gtcgcaacct
    20101 gctacggccg gcttcctgta tggcctcgta gaggctatag ccacgcttac gcaggaggtt
    20161 tgtgtagtca accagcacaa taccattctt aaccacaatc cctaccagca taatcatgcc
    20221 aacaaaagta acaatactaa gggttaagcc tgtaagcttg aaagcccaca caacaccaac
    20281 aaaggtaaca ggaatggcga acataatgat gaatggatcc ttgaagcttt caaattgggc
    20341 ggccattacc atgaaaacca gcaggatgcc aaggatgagt atcaggtaaa ggtcggcaaa
    20401 cgactcgccc tggtcggagg tttgtccggc tatctttaca tccacatcat tcgggaatga
    20461 ggttgatgaa acaatacgct gtgcctccat ctgcccatct ccaagcgaaa ccccatttag
    20521 ctcggccatc accttaacca gccgctgctg ctccttacgg ttaatctgca gcggacttgt
    20581 tccggtttca atctgggcaa cagcataaag cggaatgtcc tgattaagca ggtttttcag
    20641 ctggatattc ctgatttggt tgatgtcgtt ccggtagctg ggcgagtagc gaagcgtaat
    20701 gtcgaactta tcgccatcct ccttatattc acctgcttcg gttccgtaaa tgctttgacg
    20761 tacctgaagc ccaatcatag ctgcgttaag ccccatggcc gaggccttct gtttgtctat
    20821 tttaacttga acttcgagtt tccccttatc cacatcgttg tccacattgg taaagtagtc
    20881 cgattgcttc agcttggctg ttaacgcctc ggcagtttgg ttgatggttg aaaagttgtt
    20941 acccgagatt tcgatttcga tgggcttttt gttgcctgtc aaggctgtgg ttagcacact
    21001 tccgcccagg agggtaaaat tctcaatttc aggaatttcc gaaattcttg gacgcagacg
    21061 gttggcaatt tcgcgagctg atgcgctcct ttcgttcggt ttacgcaggt gacacatgat
    21121 agtggctacg tttttcccct cgctaaaacc tactgacgag agaattccat cctcggtttg
    21181 gccggcaata gaaaaaattg agcctggagc catctcggga atttcctcct caaagatttt
    21241 taaaaccacg ttggctacac tatccgtttt tgctgcagcg gttcctacct cagtttcaac
    21301 cacggccact acgtcgccgg catcaaattc gggaatatag tcggttccca tatttctgcc
    21361 catccaaacg gaaagaccaa gaatgatggt aacaatgata acggtaatgg ccttgtgata
    21421 aactacccac gctaaagcct ttttgtacca gccttccagc ccggttagaa gtttctcgct
    21481 ggcagtaaag aacctgttgt gcctatgtac ctctcccggt ttaatgcctt ttaccaagag
    21541 cgatgaggcc ataggggtga gcgtaagagc cgtgaagagg gaagccaaaa gggttaccga
    21601 ggtgagaatg gccagctgct tgaacatgat tcctacaatt cctcccatga aaaccatcgg
    21661 cagaaaaaca acaatggtgg taaaagttga ggctgatatg gccaaaccca tttcgctggt
    21721 accaaatatg gccgcctgtt taggcttttc gccgttttca acatgtctgg taatgttttc
    21781 aagcactaca atggcatcgt caaccaccat tcctgaaaca actataagcg acatcaggct
    21841 aaaaatgttg attgtccatc ccgcggcaaa cattacgatg aaggctacaa ttagcgaaaa
    21901 gggaatggta ataaaaatga tgaagctctg gttccagttt cgaaggaacg ccagcacaac
    21961 caaaatgacg aagaaaaggg cgtaccaaag cgtttcggat aggttgctaa tagactccga
    22021 gaccagctca tcgctcttca tcacctcgct catggtaaca tctgcaggca gtgtcttttt
    22081 tagctcatct accttaagcc tgatagcgtt ggccacatta agcgtatttt cgccactctg
    22141 cttttgaatc atcataataa gccctttacc gctggaggtt cgagggtact cgtccttctc
    22201 cttaaactca tcgtggatgt ctgccacgtc gcttagcttg ataaccttac cgttgaagct
    22261 ggtcaggggc atttccgcca actcgtcaat gctggcaaca tcgcccggaa tctgaacggc
    22321 ataatcgctg ctgccaatct tgatgtttcc tccgggtatt gagagattgg aagcctgaag
    22381 gatgttggcg atttgcgaca tggagaggtt ataggcgttg agtttaaggg ggtttacgtt
    22441 gatcttgatt tcgcgttcag gttccccaat gataattact gatccaacac ccgaaacttt
    22501 gcgcagcttg ctggctattt cgtcgtttac caacttgttg agtgcaccat agctttcctt
    22561 tgccccaatg ctgtaagcca atacaggcat tagcgagctg tttaccttga aaatagttgg
    22621 attctgggct tcgcggggca acttgttttt aaccagctct atcaggtcgc gaacattatt
    22681 ggctgccgag gtaatatctg caccccagtc gaattggagc tggacaaacg aaacgttctc
    22741 cttgctttgc gaggagatgt ttttcaggtt ctctgtgcct gaaagtatca tctccagctc
    22801 tttggtaatc tgctgctcaa cttcgctagc gctagcgccc gggtaaactg tgattactgt
    22861 tagggtggga agttccatat cgggcatgat gtcgagcggt agccgggtga gggataccac
    22921 tccaaacagc aaaacagcta taaaaagcat tgtaatggct actggccttt tgaccgctac
    22981 ttctggcaat ttcatccgtg caaaatttta atattgtgag ttggcaataa actacaattt
    23041 ggcattttat actcgttaac gttcgtgtag aacatccatt gctttgaaat cctttgtgct
    23101 acatgggtgt ttcctgtttt catcaaagta ttttttttca gcacgcatct aaaaccctaa
    23161 acgagcctat tttccgctga cccgtacacg ttgtccctga cttaccctgg ttttcccatc
    23221 aagaaccact tcaacgccgg gttcaatccc tttcaccgca acatattcgc ctattgattc
    23281 aagcctggat atcggtattc ggttaatggt gtcgttggtt accgagaaaa ggtaatcatc
    23341 cgaagtgccg ggtaggcggt aaatgctttt taatggcact acagaagcct taatttcggg
    23401 caacttcacg gtgatgtagg caaacattcc gggtttaatc tccagctgcg ggttactaat
    23461 ggtaacctcc actgcagctg agtgggtggt ggttgacaga atttgtttta tggttcccac
    23521 ctttccttgg taggttttgc ctgcaacgcc gtcgaaggta acctcaactt caagtccttt
    23581 ttttatgaca cctaaatcct tttcgttgac atcgaaccta accttaacgg ggttaagctg
    23641 catgagccta acaattcccg agcgcatgga gtagcccggc tctagcccag ggtttaggaa
    23701 gtaaacctca ccctcctcca tcatgcgctc aacaaccata ccgctgaagg gggcataaac
    23761 ccttgtgttt tttttgacca tttgtacttt ggcctgtgag gcatcatact gagccttaac
    23821 gtggtcatac tccatttcgc tgatgcttcc tttttccctg aggcgcgaaa ctcgatcgaa
    23881 atcctttttt atggtttcgt tttcaatgat tgtctgggtg agcagttcgt ctgatagctc
    23941 aactatcagg tcgccttcgt gcacgttttg cccttcgtcg aagtaaattt tttcaacctt
    24001 tccgggaaga gttgccccca ggttggcctc tcgattggca aaagctgttc cggtaaagga
    24061 aagggtgggg ctataaattc gctcctcaac cacaacagtc gaaaccaacg gaacgttttc
    24121 ctccgttgcg ggaacttctg ttttactggt cttgcaccct gttagcacta gtgcaatagc
    24181 aagtggggtt agccctagga tagtcggttt catatttgcc attattaaaa tattgttatt
    24241 ctgttaattc cttttgtttg cagctaaagg gtacctgtaa cctttttaag gttggtaatg
    24301 ttaagccggt actccattcg ggcatctata agatcggagt aggcattttg ccaaagggtt
    24361 tgtgcctcaa gaacatcgga ggttttaagt ttgccttcat caaaagcatt acgggtaacc
    24421 tttaggtttt cctccgcctg ttttaggttt tcctcggcca tttgaacctt tttcaccgat
    24481 tcgttaagct ggtaaactgc ctgctgtacc tggagagaaa tgagctcttt tgcctcatcc
    24541 attttgagtt cggcaacgcg ttgctcgcta tgagccgctt taagcgtgtg ccccctgtcg
    24601 ttccagtgaa aaattggtat attcagcact acacctacgt tccagtcgcc accaaaattt
    24661 tcggaaaatc cattataggg gtttgggttg aaaagcatgt agtttgccga aagcccaatg
    24721 ttgggcatga agcgcgaacg catcaggttg acacccgatt tggcaatgtt gatggtttgg
    24781 tttagcattt ctatttctgg tcgtttcgcc agagccgtat cggtaaaact ttgcatgata
    24841 acggctgtat ccatggaggc taccaacgag tcggccaaaa ggatttcctg gttcagggga
    24901 aggcctacct gctggcaaag ggccatttta gaaagggcaa ggccgttctg cacctttacc
    24961 aggtttagct gagcttcatt tagcttcacc tttaccttga gcaggtcgtt ttgaataacc
    25021 attccctcct cgtaaaagtt ttcaatatca acgctaagct tctctagaag ctgaatggag
    25081 gcatttacca gctttacctt ctccccaact gctaccaccc gccagtaggc ctcttcagtt
    25141 ttgtagatga tatcggagcg ctcggctgcc tcgttggcac gtgccaggct ttctccgtac
    25201 ttggcaatcc ggtatgtttc cctaattttt ccaccggtaa atatgggttg ggtcaaactt
    25261 actccggcgg tgtaaacatt cttatagcct acttcggcct gatcttttgg aagccatgtg
    25321 tagttttgaa aaatcggatt gccattggca tcggttaccg gctgaagcgt tgaggggttt
    25381 attacaaaag tattcgccgg atcgagattt gggttaaagc tgcccgtact cgggtcgatt
    25441 gcgctgtagg ggattaccgg aaggaacaag tcgttctcaa acaagctgaa ctttttgttg
    25501 gtgcgcatgt acaaaccatt tgcacttaca ctcggaaaga actgggtttt ggccgaactt
    25561 ttcagcgagg ttgctgcctg tacatgctca ccagcaatga gcatccgctg gttatgctcc
    25621 aatgccattt ggcggcattg ctcaaccgtt agcagctgct gggcgttgga cactccgtcc
    25681 gctagcacca gcaacacaaa aatatacaga agccgtttca tccattcagg tatttatagt
    25741 attaataaac agttcgttgt aatattgcac atttatagct gaagtcgttc acacaaaaag
    25801 tgatcaacca tttcgggtct gctgttcatt cctttttgtt tgttattgct acgaacgtca
    25861 caacagttta cattttccaa ccatacatga atacgtcacg ttaaatgcca tcaacacacc
    25921 cggttaacgt ttaacgatgc cgaagaaaag aatatccagg aggtcgtcaa taaccgggtt
    25981 catggcattc tcatcgtttg tagagttaac gaggggtagc tccaaacctt tcatagccgt
    26041 tacaattgcc agtgctgaca tgtagaaatc ttttacctta aaagccccgc tacggctacc
    26101 ctcctcaaga atttgggtga tgatggaaat ttcctcatca tcgtattttt tccgaaattt
    26161 ctcggttagc tgcaggcttt ttaggtcgtc gttcagcctc tgttccacga aactcgacag
    26221 gtgccgtata aactccatac gtagcttaac gtacgagcgc atttttgata ctgcatcatc
    26281 ggggctttcg agtattttct ttacattttc tctttgttgg cttgcttcgc gtgcaacaac
    26341 cgccctgaaa atctcctcct ttcccttaaa gtagtagtag attgaacttt tgcccattct
    26401 caagctacgg gcaatatcgt ccatggaggt tcttctaaat ccaaagcggc tgaacgcctt
    26461 tgtggcggca ttgataatgc tattccgcat ttcatcatgc gggctgccaa tcacttcctg
    26521 atgcatggtt aaatattcga ctgttttgtt aaaacgttcc aaaattacta cgtaattatt
    26581 ttttgtcaag gaatattcga ctaaaaagtg ttaaaattcg aataaatgtt ttatgttgct
    26641 gataaccttt gagttattcg atagttttag aaatgtttat tggaaagcga atagaaggaa
    26701 gcatgaagcg ataactgcta accgggtata gcttaattgc agcgtttaac ctgtagagta
    26761 gggaatccgc gggcaggcaa acgagtcttg gaattacttt atcctttaac cctaaacctt
    26821 tatcctttat tttccctcct tccctctttc agaaagattt gctaaaaat
//
LOCUS       DAOSHM010000035        23814 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_1681_length_23814_cov_21.975041, whole genome shotgun
            sequence.
ACCESSION   DAOSHM010000035 DAOSHM010000000
VERSION     DAOSHM010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 23814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23814
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1681_length_23814_cov_21.975041"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..822
                     /gene="purQ"
                     /locus_tag="PK839_06890"
     CDS             <1..822
                     /gene="purQ"
                     /locus_tag="PK839_06890"
                     /inference="COORDINATES: protein motif:HMM:NF002957.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HOG72495.1"
                     /translation="HFKAKLLKGVKLLYFNLYINVDFACKLLIPIFEKFLDEKPMKFG
                     VVIFPGSNCDRDTIYVLREVLEQEVVTLWHKETDFDADVDAVILPGGFSYGDYLRSGA
                     LARFSPIMTRIAEFASNGGYVFGICNGFQILCEAELLPGALLHNASRQFVCKNVYIAP
                     DSKIAPPTAFLPKGRVFKIPIAHGEGRYFADRETLARMRVNGQIIFRYCDETGVISEL
                     ANPNGSVENIAGVCNERKNVFGMMPHPERASDDELGNTDGRLIFESIIKFIQGNG"
     gene            1252..3843
                     /locus_tag="PK839_06895"
     CDS             1252..3843
                     /locus_tag="PK839_06895"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG72496.1"
                     /translation="MSRNYHNLTSYYNYYYNAYDSYRNGERRVYKDFKYNYTLPLPML
                     LFGERGVSDMVSSDMERTLNKCTALLAKHSITVKPKRKNGSLSSKEKEFYNRYEYVRW
                     AQESWLLVGKAHTWKGDLSKAQMAFEYVIRQFPNTTTWYEAQVWLARVEILNGDFISA
                     GDRLKSVDAKRRKPTDRQFNHLLSSTWAFYHLKQHNSNEAIPFIKRAAEFTNYKAERL
                     RYTYLLAQLQQQLGNTTEAIELYDRVMRMGPPYEMAFSIRIQLIALSNMKGEDLKREL
                     LKLASDEKNKDYLDQLYYTLGNMEKQEGNMDKAIEYYLLSARLSTTNDNQKGMSYLVL
                     ADHYFQKSEYAKAQAYYDSSFNSLDASFPGYAQLETKTRFLTNLVENLNIVSTEDSLQ
                     RVAAMSPKDRDALIASIIGKLQEEEQKLKAQEQADQMQSQIYMQNQRFRSNSEPQGGK
                     WYFYNPASLSYGQSEFQMKWGKRKLEDNWRRKNKGVSDFENIASGGLPSDSAANPQKQ
                     LSNTSPEFYLVNLPFTDSLKQISNQKILTAMMRIAEIYQTDLNDSKAAKESYKKLSER
                     FPDASEAATAIYRMYRIAADEGNSVEAEGYKIALLSRFPSSPYALILSNPEYIKELTV
                     KENEANSIYETAYGLYLQGNFSEAAKTLKSGMGKVKGTDMEPKYLLLDALCQSKTGDL
                     RTFKESLEQVALTFPKSEQGQVAQNLLTYLEQRDLQLSTGKVSIDVPVVSVSSDSIST
                     SIYTKPEGEHAFVLLVPKRTNINQLKFNIITFNVDNFIEMDLALNSVPLNDFVEVVSV
                     TGFQSADIALSYYKLITQDEKVFADFNRAEFQAFLFSTENFNRFMNDKSIPDYSRFFK
                     ENYSVDN"
     gene            3853..5412
                     /locus_tag="PK839_06900"
     CDS             3853..5412
                     /locus_tag="PK839_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional response regulator/alkaline
                     phosphatase family protein"
                     /protein_id="HOG72497.1"
                     /translation="MATPASILWVDDEIDLLGSHIIFLEQRGYHVQKAINGVDAIELV
                     RNNVFDIVFLDENMPGLSGLETLTQIKELKPHLPVVMVTKSEEENIMDMAVGSQIADY
                     LIKPVNPNQILLSIKKHVHGKTLVSAKQTSDYGVEFSQLSEQIAAAQSYSDWVELYRR
                     LSAWQLRISDNSEPNLRQMLDMQMAEANQLFSRYVRSSYIGWFKGNQDDKPLLSPNLL
                     RSKVFPLLDSGEKVLLVLIDNLRYDQWKAIEPLLADEWRVESDDVYFSILPTATQYAR
                     NAIFAGLMPLEIQQMFPDIWISDDDDEGKNNYELQLLTSQLQRLGKSYKVHYEKGNTL
                     RAGMPIADNVNLVLNNNLTVLVINFVDMMSHARTDSNMMKELASDEAAYISLVRSWFQ
                     HSGLRNILLEAARFDIKVVITTDHGSIRVQNPIKVVGDRATSTNLRYKLGRSLAFNTK
                     EVFEVRDPETAHLPKPNVSSSFIFAYGSNFFVYPNNYNYFASYYRNTFQHGGISLEEM
                     IIPVVVLTPNR"
     gene            5636..6064
                     /gene="tsaE"
                     /locus_tag="PK839_06905"
     CDS             5636..6064
                     /gene="tsaE"
                     /locus_tag="PK839_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004379084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HOG72498.1"
                     /translation="MKSLLIPTIEALDSVASEVAEIVKQYHVVCFYGEMGAGKTTLIK
                     AICHALGVKDIVASPTFALINEYQTRSGQPVYHFDFYRINRIEEVYDFGYEEYFFSTT
                     GICLIEWPELVEDILPSDQTVKLFITVNPDFSRRLDIMVD"
     gene            6370..7590
                     /locus_tag="PK839_06910"
     CDS             6370..7590
                     /locus_tag="PK839_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HOG72499.1"
                     /translation="MKRTASNLPEFPHAKGLLVQEERLEFVKKNKQLVIGIPKEADST
                     ESRIPLTPEAVEMLVEGGHEIIIEKDAGKQANYNDLDYSEKGGRIVTSSQEVYRSDIV
                     LKVAPLIPSEFELVRDNQIIMSSLHLNSSTGDYLRKLIQKKVTAIAFESICDSDGSFP
                     FIRAMSSIAGSTSVLVAAEYLSNVNKGKGVMLGGITGISPTEVVILGAGTAAEFAARA
                     ALGLGATVKVFDNSIKRLMELQNLLGQRLYTSIFHPQVLARVLKTADVVIGTLTPEES
                     NLRFVVSEEMVMNMKHGSVIVDLAIDKGGCFETSEIRDHNNPSFEKYGVVHYCVPNIT
                     SRVARTASIAMSNVFAPLLLSIGEAGGFQHQLKEDSGLRHGVYVFNGILTNDYLGRLY
                     NLPSRDINLLMAAF"
     gene            complement(7605..8360)
                     /locus_tag="PK839_06915"
     CDS             complement(7605..8360)
                     /locus_tag="PK839_06915"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HOG72500.1"
                     /translation="MNSWFKSLSASIAKHRVGILTTIIIHLLVIASFLVFKIEDRTEE
                     YASTIEMEFEEPKAQEELLVEEKPEQLLPTDVTVPSREMEAIKNFAVDATQKELNAGL
                     SDEKNTDADNLYSEANRIREQMQENKERYEQAMKEADIAIPNTPEKNIPEEKKNMYKG
                     PTVISYNLAGRKAYRLPVPSYKCEMGGQVVVNIEVLSDGSVSNATIDWANSVNDECIN
                     NSAIMAALASRFSSTQQSTGKEKGSITYLFVAQ"
     gene            complement(8456..10243)
                     /locus_tag="PK839_06920"
     CDS             complement(8456..10243)
                     /locus_tag="PK839_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HOG72501.1"
                     /translation="MKPIIQATSISTVTYISNLLIGLTIALTQFFTAEAYSQDTLKTY
                     CNPINIDYGYSPIPAAIEQVKHRATADPLVVAFKGKFFLFSTNQHGYWWSSNMASWNF
                     IPRKFLLPRHKVYDDLCAPAAFVLGDSLLLIGSSNNPDFPIWASTNPAADDWHIAIDS
                     FPLAAWDPAFFADDDGRVYLYFGSSNLYPIYGCEVNRATLRPQAAPKPLISLNDSIHG
                     WERFGEYNDNTFLPPFIEGAWMNKHNGHYYLQYAAPGTEFSGYADGVYIGSNPLGPFR
                     YQEFNPFSYKPGGFARGAGHGATFCDYNQQWWHISTIAIGVKNNFERRIGMWPAYFDA
                     DGTLYSQTAFGDYPHYLNGKFTEWMLLNYQKPVTVSSTLYGYYPNFAVDEDIRTYWSA
                     STGNAGEWICTDLLDTCRIYAFQVNYADQDVQLVGKQMGIYHQYRVWGSLDGKSWSLL
                     IDKSENKTDVPHDYVELSTPVEFRFLKLENLHVPAGKFAISGFRVFGKANLPLPESVE
                     FFSVLRGNSEPRNAWLKWKLQDNATGYLINFGVRPDKLYGSIMVYGHNEYYFSGMDRD
                     KTYYFTITPFNESGCGNRSAVVKSEIPLR"
     gene            10519..11724
                     /locus_tag="PK839_06925"
     CDS             10519..11724
                     /locus_tag="PK839_06925"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005841782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HOG72502.1"
                     /translation="MLSIREVYKEGYGPSSSHTIGPVRAAELFIKKHPEVSYFRVMLF
                     GSLALTGRGHQTDSALRKCFLPKSVEVVFKPEETLPLHPNGMVFEALTDEGAEALDRW
                     EVYSIGGGDLKDKSGVLNSSEVYSLNNMTDILTWCRREGKTFWEFVEDNEGKDIWEFL
                     QGVWHTMRETIKRGIENEGVLPGPIKLARRASSQYSKALTSHGTLKSFGLLFSFALAV
                     AEENAAGGRIVTAPTCGSSGVLPAILFYLSREEYLTEVKIHRALATAGLIGNLVKHNA
                     SISGAEVGCQGEIGTACAMAAAAMVQVLGGSPNQIEYAAEMGMEHNLGLTCDPVLGYV
                     QIPCIERNAIAASKAYSCGVYALSSDGGHKISFDRAVETMAETGRDIQVAYRETGIGG
                     LAKNWEPFE"
     gene            complement(11840..12562)
                     /locus_tag="PK839_06930"
     CDS             complement(11840..12562)
                     /locus_tag="PK839_06930"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HOG72503.1"
                     /translation="MRRFMITGTFALMTSMGSYAYAQGQVTIQNDSKDFVGVKNSISA
                     TVNLYQASTYKVEITTSQKVADKLEISVENGLLSIREKDQSWNHWSCSNNTDDEVVVN
                     VWSPKYSTLEINGSGDMLAKTPIKTNEIEVKVNGSGDVKINELEAEKVSLKTNGSGDI
                     AVSGKKEASTLVIKINGSGDIDTRNLVTRVADVAITGSGDVSANVLEKFTANSVGSGD
                     IYLSGNPQVDAKILGSGEIVRK"
     gene            complement(12789..13076)
                     /locus_tag="PK839_06935"
     CDS             complement(12789..13076)
                     /locus_tag="PK839_06935"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72504.1"
                     /translation="MLEKKLYANGQPVYKLADGMLIFYYKNGTVKAEGPYPDNMMEGE
                     WRFYRESGQLWQIGNFKNNQKHGSWIRYDRNNNVEYQETFEHNKAVNPKRR"
     gene            complement(13268..13708)
                     /locus_tag="PK839_06940"
     CDS             complement(13268..13708)
                     /locus_tag="PK839_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007211929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HOG72505.1"
                     /translation="MTLNEVQDAIVEDFSISNDWMDRYQQLIELGKELPPIDEKKRTD
                     QYLINGCQSKVWLDAELRDGRIFFSADSDAIITKGIVSLLIKVLSGRTPEEIINADLY
                     FIDKIGLRENLSPTRSNGLVAMVKQMRMYALAYQTKLNQQKDNH"
     gene            complement(13761..14150)
                     /locus_tag="PK839_06945"
     CDS             complement(13761..14150)
                     /locus_tag="PK839_06945"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HOG72506.1"
                     /translation="MLKLELKETATTPYINFDPTSGVIRMEGRSIPENVIDFYQPILG
                     WVEEYGKNIQPETTVHLKFEYFNTSSSKRIFDLMKKFEKIGIETGTKVTINWYYEEDD
                     EDIFFAGNDYKALINKVEFNLIEIKPS"
     gene            complement(14190..14702)
                     /locus_tag="PK839_06950"
     CDS             complement(14190..14702)
                     /locus_tag="PK839_06950"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HOG72507.1"
                     /translation="MGILVEKSADCPYINFSEDGILEIEGRSITEDPFTFWQPLLEWV
                     ETYTKHPSPKTEVIIYMEYSNSSSNKYINELLRKLEECHRKTSSVRVTWRYEQDDESI
                     YQLGRDFESMLHVPFEFIEVNTSADSSKRIRVKNKSNGSEAIITHRYWEAIVRNGHGE
                     DYQILQEFSN"
     gene            complement(14871..16349)
                     /locus_tag="PK839_06955"
     CDS             complement(14871..16349)
                     /locus_tag="PK839_06955"
                     /inference="COORDINATES: protein motif:HMM:NF026018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HOG72508.1"
                     /translation="MKKIPEVSNRFFSTNLFLLLLLNLIVKPFWIFGIDRSAQNALGA
                     SDYGLYYALFNFTLIFNILLDCGVTNFNNREISFHPQLAGKYFANILSIKLMLSVVYV
                     AITVAVALGMGYGHHQLGLLFILAVNQFLSSLVMFIRSNLNALHHFGLDSVLSVLDKL
                     IMILLGSVLLWTPLRKNFTIEAFAVAQTLSYMATVAVSLIFLTQTTGRLRIKFDRVLS
                     LSMLKNSLPYALMVLLMTAYSRLDSVVIDRLLPNGSEAAGIYAQAFRLLDAANMLPFL
                     FASLLLPIFSGMIKQKREFGPFLNFSFLLLVVPVVAIAIPTIAHRAPIINLLYHEHVD
                     LSAPVLGLLMISYIFISVGYVFGTLLTSNGNIKELNIVSGAAVVVCLSVQITLVPRFG
                     VTGAAIGNMVVNFLVAVAQVTISLKKFRLTPDRTMVFRFLAYLLFCPLATVSILNLHL
                     GMAFSYVLSIAVSLGFALVLKLIDIKQLLATFTIENTPNVKG"
     gene            complement(16648..17691)
                     /locus_tag="PK839_06960"
     CDS             complement(16648..17691)
                     /locus_tag="PK839_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008860964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOG72509.1"
                     /translation="MYRLFLRNVLAVFLMLVALGYSEAQEKKAYKVSCIGFYNLENLF
                     DTLPGPNDVEFTPDGPNKWNSERYYHKLNNMAEVIAQIGDEMFPGGPAVLGVSEIENR
                     LVLDDLVNTPRLKQSNYQIVHYDSPDRRGVDVGLLYRPEFFKVTSHRSVRLNIPDNPE
                     FRTRDQLVVSGLLDGETIHIIVNHWPSRSGGEKRSQPYRIAAAKLSRSIIDSIRATDA
                     DAKIILMGDLNDDPDSRSIIDYLGAKNNAEGLRSGDLFNVMASLYREGIGTLAYQDSW
                     NLFDNIIITQPLLSKDRTSWVFYKAKVFNKPFLRQTSGQYAGYPFRTYVGANYSGGYS
                     DHFPVYIFLIKEK"
     gene            17991..20714
                     /locus_tag="PK839_06965"
     CDS             17991..20714
                     /locus_tag="PK839_06965"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOG72510.1"
                     /translation="MKRLLTTVLSLFLLGMGSLYAQNTLRGVVKDSDTDTPVQDVRVR
                     LLGTTFETLSNAGGEFTLEGVPDGKFTLEFSIVGYQTITQEVSLPENSQVPVVSLRRE
                     VVADNGSQEFSEVVISADDLDSDNMDQAISGLLQSSQDAFNSAVSYTFSPASFKIRGY
                     DSDYSLLYMNGVPVNDPEIGFASWSSWGGLNDVVRSRESRNGFLPTNFSFGGLGGATN
                     LNVRASQQRVGTRLSYAATNRTYNNRIMFTHSTGVMENGVSFTISGSRRWAEEGYVDG
                     TNYDAWAYFIGIEKKFNSQHSVAFTTYNAPTKRGMQGVSTEEAKNLAGSSYYNPNWGY
                     QNGEKRNSRVRFQQEPTFILNHYWNMLPNLALTTTAGYSFGTYQTTALNWYDAQDPRP
                     DYYRKLPSYWIDSDPDVSQAIADAFSNDPAISQIDWDYLYQVNYNSLDTASNLLRSKY
                     IVENRITDSRQFSLTSVASWNPTQEMKVNGGIEMTLYKGRNYKQIDDLLGGNYWLDID
                     QFVERDYFDNANLAQNDLDNPNRMVAKGDVFGYDYTANVNNGNLWAVANYTLNRFEYY
                     LGGSYTYTEFWRTGDMRNGRFPSNSKGDSPKQQFNDFGVKGGATWKISGRHYLDANMA
                     YLTRAPFFRNSYISPRTSNFVIPGLGSEKIASGDINYNLRTPYIKARITAYYTRFYDQ
                     TELKSFYDDYYRTFINYTMRNIDKEHKGFEIGAEIKASPTLTVSTAAALGSYQYKSRP
                     LATITQDNLGSVLAVDQVVYIKNFFVPNTPQTAAMVGLRYASPKYWFLGATFSYFDDI
                     YIDFAPGKRTAEALVGLEPNDPRRYDLTHQESVSSAYLLDANIGKSWRIKNYYINLNF
                     QVANILDNTDFKTGGFEQARYSIAEQTSDKFPPKYFYAYGRTYYLIFGFRF"
     gene            20746..21612
                     /locus_tag="PK839_06970"
     CDS             20746..21612
                     /locus_tag="PK839_06970"
                     /inference="COORDINATES: protein motif:HMM:NF040146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5689 domain-containing protein"
                     /protein_id="HOG72511.1"
                     /translation="MKLTKYFAATVVLIAMVTLSLPGCVDFSFDDPQPSIDSTSMSAN
                     TTIAELKSVYPGSLFQLSDTTFYLRDSIIIEGTVISDDKEGNFYKSIFIEDSTGGIEV
                     KLNKTTLYNDYKRGQKVVVLCNDLYLGDYGGQIQLGSTYKNNGVVEISGLEGDPIIRE
                     HVFKKGKTIQERIPTVLSVTQLNPTYIGMLVKFENVQIRDTISPITREIYTYADNVNK
                     VTVNHSIHQCGQAFDKLVLRTSGYARFASKPIQTKNGSITGVLTYYNGTYQLLIRDTN
                     DVDFTSNRCSNK"
     gene            21627..23693
                     /locus_tag="PK839_06975"
     CDS             21627..23693
                     /locus_tag="PK839_06975"
                     /inference="COORDINATES: protein motif:HMM:NF040146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5689 domain-containing protein"
                     /protein_id="HOG72512.1"
                     /translation="MIKKILAILVPIAMVGLIALQNGCVKEDFDTVPPLRKAISWKGT
                     ATIADVKGFFNGPNNPSRTAGLIKKLVPASYWEQLAANGVGDSSIIIEGLVISCDSAA
                     NFYETVTIMDSTGGIDFKINASDLYLTYGFKPGKRVLVKLNDLAIDSYHGVYQIGLPY
                     TDAGTLKVTGVDPSMVAACTQMSGEQANLTPLSVDIGTITAGYVQKLIVIDSVQFWNP
                     EATYSLPGVNTNRTLVDTKGNQIVLRNSGYSKFVNDKVPSGSGYIVGVLGIYDGTYQL
                     TIRDPRDIQFNNPRFGSQPPAKNATIADLKAACTSNLVKITQDLVVEAVVLANDKSGN
                     IYKSLFIEDESGAIEFKIDVNDLFIDFPVGTKIAISCKDMYVGKYGGVVQLGGVYNNA
                     IGRISANEFYQKVFVVGSGFEVTPIPVSINELTADLVGKLVTISDVQYIDSELGKTYA
                     ESSITNRHVEDALGRTAIVRTSNYADFAGITLPSNRGSITAVLSKYYNDYQLYIRDLS
                     DVWMTQPRRVKNYILKQDFSSATANSPISVGGWQSIAEVGTRRWMAKVYSGNTYAEMN
                     PYQSGEASNIGWLISPAIPVASGVTTYLTFNTQFAYWANSTLEVFVSENYDGANPASA
                     TWTKLSNAHIVTQSEGQNTWVSSGLVDISEFPGTIYLGFKYSGGGSIGQTTAFRVDDV
                     NVFTQQ"
BASE COUNT         6435 a         5391 c         5525 g         6463 t
ORIGIN      
        1 cattttaagg caaaattact taaaggtgta aagttactat atttcaacct ctacatcaat
       61 gtagattttg cgtgtaagct attaatacct atatttgaaa agtttttgga tgaaaaacct
      121 atgaagtttg gagtagtaat ttttcccggg tcaaactgtg atcgtgacac catttacgtg
      181 ctacgcgagg tgcttgagca agaagtggtt actctatggc acaaggaaac cgattttgat
      241 gccgatgttg atgcagttat acttcccggt ggtttttcgt atggcgatta cctacgttca
      301 ggagctttgg ccaggttttc gccaataatg acccgaattg ccgagtttgc aagcaatggg
      361 ggctatgttt ttgggatttg caacggtttt cagatactct gtgaagcaga attactaccg
      421 ggtgctttgt tacataatgc aagccgtcag tttgtatgca agaatgtata catcgctccc
      481 gacagcaaaa ttgcaccccc tactgcattt cttcccaagg ggcgagtctt taaaatccca
      541 attgcccatg gcgaaggccg ctactttgcc gacagggaaa cacttgcccg tatgcgggtg
      601 aacgggcaga ttatttttcg ctactgcgac gaaaccgggg ttatctccga attggcaaat
      661 cctaatggct cggttgaaaa cattgctggt gtttgcaacg aacgcaagaa cgtttttggg
      721 atgatgcctc atcccgaaag agcctcggac gacgagcttg gtaacaccga tggccgcctt
      781 atcttcgaat caataattaa gttcattcaa ggtaacggct agcctggaaa tttgataatt
      841 cctccatttt tgttacaacc cggcagtccc tctttcagac cgagatggaa ggtttgttta
      901 ccccacaaca atagtttacc gccacaaacg tttttagcat acaatatctt tttgaactgt
      961 ggctgttctt aacggtttcc taccatctta tggttgtttg atggaataaa agtttactgt
     1021 ttgttggaca gctgaattgt tgctaagctc gaggctgccc tggagggcaa ttcaactttt
     1081 attgctaata ttgtgggcta tataaatttc aacattccgt ttaattcaac tcggcatact
     1141 ttgcttttga ttggggaaac agctggtact atgcgcttat tgaccagaaa catggcagct
     1201 tgcatgctga ctttagttct tgcttcttgc tcaacccaga aaaatacctt tatgagcagg
     1261 aattaccata accttacctc ctactacaac tattactata acgcatacga tagctaccgt
     1321 aacggcgaga ggcgagttta caaggatttt aagtataact acacgcttcc ccttcccatg
     1381 ttgctgtttg gagagcgtgg tgttagcgat atggtttcct ccgacatgga gcgaaccctg
     1441 aacaaatgca cagcattact tgccaagcac tcaataactg taaagcctaa acgtaaaaac
     1501 ggttcacttt cgtccaagga aaaggaattt tataatcggt acgagtatgt taggtgggca
     1561 caggaatcat ggcttttggt tggcaaggca catacgtgga agggcgacct gtccaaagcc
     1621 caaatggcct ttgagtatgt tatccggcag ttcccaaaca ctacaacatg gtacgaggct
     1681 caggtatggc tagccagggt tgaaattttg aacggtgact ttattagcgc tggtgatagg
     1741 ctaaagtctg ttgatgcaaa aagacgaaaa cctacagata ggcagtttaa ccacctgcta
     1801 tcatcaacat gggcattcta ccatctaaaa cagcataata gcaacgaggc cattcctttc
     1861 attaaacgtg cagctgagtt tacaaattat aaagccgaac ggcttcgtta tacctacctg
     1921 ctagctcagc tgcagcagca gcttggcaac actaccgaag ctatagaact ttacgacagg
     1981 gtgatgcgga tggggccacc ctacgaaatg gcatttagca tacgaattca actgattgcc
     2041 cttagcaaca tgaagggtga agacttgaaa cgtgagctcc tgaagcttgc ctcggatgaa
     2101 aagaacaagg attatctcga tcagctttac tacacgctgg gtaacatgga aaagcaggaa
     2161 ggcaacatgg ataaagctat tgagtactac ctgctttctg ccaggttgag caccactaac
     2221 gataaccaga aaggcatgtc gtacctggtg ctggccgacc actactttca gaaatcggag
     2281 tatgccaagg cacaagccta ctacgacagc tcattcaact ccttggatgc aagtttcccc
     2341 ggatacgcac agctggaaac caaaacccgg tttctaacca atctggttga aaacctgaac
     2401 attgttagca ccgaggacag ccttcaacgg gttgcagcta tgtctccaaa agacagggat
     2461 gcacttattg cctccatcat tggtaaactt caggaggagg agcagaagct aaaggcccag
     2521 gaacaggccg atcagatgca gtcgcaaatc tacatgcaga accaacgatt cagaagcaac
     2581 tccgagcctc aaggcgggaa atggtacttt tataatcccg cctcgcttag ctatgggcaa
     2641 tcggaattcc agatgaaatg gggcaagcgt aagctagagg ataactggcg cagaaagaat
     2701 aaaggggttt ctgacttcga aaacattgcc tctggcggct taccatccga ttctgcagca
     2761 aatcctcaga agcaactttc aaacacctcg ccagagttct acctggttaa ccttcccttt
     2821 accgattcgc tcaaacaaat ttcgaaccag aaaatcctta ccgccatgat gaggattgcg
     2881 gaaatttacc agacagacct caacgacagc aaggccgcca aagaatcgta caaaaagcta
     2941 tccgagcgtt tccccgatgc ttccgaagca gctacggcta tctacaggat gtatagaatt
     3001 gcagctgatg agggcaatag tgttgaagcc gaaggctaca aaatcgcgct actttcccgc
     3061 ttcccttcaa gcccttatgc gctaatactt tcaaaccccg agtatattaa ggagctaacg
     3121 gtgaaagaaa acgaagccaa cagcatttat gaaacggctt acggtcttta ccttcagggc
     3181 aatttcagcg aagctgccaa aacgctaaaa tcggggatgg ggaaggttaa aggtaccgat
     3241 atggagccaa aatacttgct acttgatgct ctctgccagt caaagactgg cgacttgaga
     3301 acttttaagg agtcgcttga gcaagttgcc ctgactttcc ccaagagcga acaggggcag
     3361 gttgcccaga atttgttgac ctaccttgaa cagagagacc tccagctttc tacaggaaag
     3421 gttagcattg acgtgccggt agtttcggtt agctccgaca gcataagcac atccatttac
     3481 accaaacccg agggagaaca tgcttttgtt ctgcttgttc ctaaacgcac caacattaac
     3541 cagttaaaat tcaacattat aacatttaat gtggataatt ttattgagat ggatctggct
     3601 ttaaacagcg taccgctaaa cgactttgtc gaggtggtaa gtgttaccgg gtttcagagt
     3661 gccgatattg ccctaagcta ctacaagctg attacgcagg atgaaaaggt gtttgccgat
     3721 tttaaccgtg ccgaatttca agctttcctt tttagcaccg agaactttaa tcggttcatg
     3781 aacgataagt ctatcccaga ctattccaga ttttttaaag aaaactacag tgttgacaac
     3841 taacaaaatg ctatggcaac accagcatct atactttggg ttgatgacga aatagaccta
     3901 ttgggttcac atataatctt tctcgaacag cggggttacc atgtgcagaa ggccatcaat
     3961 ggggtggatg ccattgagct ggttcgtaac aatgttttcg acattgtttt cctcgatgaa
     4021 aatatgcccg ggctaagcgg gctcgaaaca cttacacaaa ttaaggagct aaaaccccac
     4081 cttccggtgg taatggttac caaaagcgag gaggagaaca taatggacat ggcggttggc
     4141 tctcaaattg ccgactacct gattaaacca gttaatccca accagatact cctttccatt
     4201 aagaagcacg tgcatggtaa aacgctggtt agcgctaagc aaacttccga ttatggggtt
     4261 gaatttagcc agctgagcga gcaaattgct gctgctcaaa gctactccga ttgggttgag
     4321 ctctaccgta ggcttagcgc ttggcagcta agaatttctg acaatagtga gccaaaccta
     4381 aggcagatgc ttgatatgca gatggccgaa gctaaccagc tattttctcg gtacgtgcga
     4441 agtagctaca ttggctggtt taagggtaac caggacgata aaccactatt atctcccaac
     4501 ctccttcgca gcaaggtttt tccgttatta gattccggcg aaaaggtcct cctggttctt
     4561 attgataacc ttcgctacga ccagtggaag gctattgaac ccctgctggc cgacgagtgg
     4621 agggtagagt cggatgatgt ttacttcagc atcctgccca ctgctaccca gtatgcccgg
     4681 aatgccattt ttgcggggtt gatgcctctg gaaatacagc aaatgtttcc cgatatttgg
     4741 atttccgacg atgatgatga ggggaaaaac aactatgagc tccagctgct cacctcccag
     4801 ctgcaacggc tcggaaaaag ctataaggtt cactacgaaa aaggtaacac cctgcgggcg
     4861 ggcatgccca tagcggataa tgttaacctg gtgctgaaca acaaccttac cgtgctggtg
     4921 atcaactttg ttgatatgat gtcgcatgca cgcaccgact ccaacatgat gaaagaactg
     4981 gcctccgacg aggcggccta catctcgctg gttcgatcat ggttccagca ctccggtttg
     5041 aggaatatcc tgctcgaagc tgcccgtttc gatattaaag ttgtaatcac caccgatcat
     5101 ggttcaatta gagttcaaaa ccccattaag gtggttggcg atagggcaac ttcaactaac
     5161 ctccggtata agctggggag aagtttagcc tttaatacca aggaggtgtt tgaggtgcga
     5221 gaccccgaaa ccgctcatct gccaaagcct aacgtaagtt cctcctttat ttttgcctat
     5281 ggaagtaatt tttttgtgta tcccaacaac tacaactact ttgccagcta ctaccgtaac
     5341 acctttcagc atgggggaat atcgttggag gagatgatta ttccggtggt agtgctcact
     5401 ccaaacaggt agcccatgtt accaatcgag aaatgcagcg gaagtagatg gttaaaaaac
     5461 tcaattgacc gacgattcat ccatacccga caacatcact ataaaccaaa gtaatctatg
     5521 cactgccagc tagcttgcta tacgtgtaaa tttcgtacag ttatttcata tcccgctcta
     5581 tgaatacaac taccacgctg gctatagcaa aacaataaag gaaacccatt tcagaatgaa
     5641 gagcttgtta attccgacaa ttgaggcgct tgatagcgtg gcctccgagg tggctgaaat
     5701 tgttaagcaa taccatgttg tttgttttta cggcgaaatg ggggcaggga aaactaccct
     5761 cataaaggca atctgccatg cgcttggcgt taaggacatt gttgcaagcc caacatttgc
     5821 gctgattaac gagtatcaga ccagaagcgg gcagcctgtt taccattttg acttttaccg
     5881 aatcaaccgt attgaggagg tttacgattt tgggtatgag gagtattttt tttccaccac
     5941 aggaatctgc ctcatcgaat ggcccgagct ggttgaggat attttacctt ccgaccaaac
     6001 tgtaaagcta ttcattaccg ttaatcccga cttttcccgg agacttgaca taatggttga
     6061 ttaacctttt tttgtgtaaa aatcgatatt actataactt tacttcacgc agattcgtat
     6121 ataatagccc aagtctggcg ggattgagag caaatcttac gaatgtttat cttttaggat
     6181 atcctctcag ctttagaaca accatcatga aatgcccatg taagggagct tcctcaaata
     6241 cggttcaacc accgagcgtt acttacgagt tcggcgaaac cagaatatgg gttttcaata
     6301 agttcaaccc cgaaggttaa aaggtatggg gtaagataat tgcaatcaga aaccaaaaat
     6361 tttgtacgga tgaaacgtac tgcatcgaat ttacctgaat ttccacatgc taagggactt
     6421 ttagttcagg aggaaaggct ggaattcgta aagaaaaaca agcagctggt gatagggatt
     6481 cccaaagagg ccgattcaac agaaagcagg atacctttaa cccctgaagc cgtagagatg
     6541 ttggttgagg gcgggcacga aatcatcatt gagaaggatg caggaaagca ggcaaactac
     6601 aacgacttag actatagcga aaagggtggg cgcattgtta cctcttcaca ggaggtttac
     6661 cgcagcgata tagttcttaa ggtggctccg cttattccct ccgagttcga gcttgttagg
     6721 gataaccaga taatcatgtc gtcgctccat ctaaacagct ccactggtga ctacctccgt
     6781 aagctaattc aaaaaaaggt taccgctatc gcctttgaga gcatatgcga ttccgacggc
     6841 tcctttcctt ttatacgggc gatgagctcc attgctggaa gcacgtcggt gcttgtggca
     6901 gctgagtatc taagtaatgt aaataagggt aagggtgtta tgctcggcgg tatcaccggg
     6961 attagcccca ctgaggtagt aatactgggc gccggaacag ctgccgagtt tgctgctcgt
     7021 gcggcattgg ggcttggcgc tactgtcaag gtgttcgata actccattaa gcggctaatg
     7081 gagcttcaga atttactggg gcaacgtctt tacacctcca tattccatcc gcaagtgctg
     7141 gcccgggtgc tgaagacggc cgatgtggtg ataggaactc ttaccccgga ggagtcgaac
     7201 ttgcgcttcg ttgttagcga ggagatggtg atgaacatga agcatggatc cgttattgtt
     7261 gacctggcca ttgataaggg tggttgtttt gaaacatctg agattcgaga ccacaacaac
     7321 ccctcgttcg aaaagtatgg cgtggttcac tactgcgtgc caaacattac ctcgcgggtt
     7381 gcccgaacag cctcaattgc catgagtaat gtttttgcac cgttgctgct atctattggt
     7441 gaggctggag gtttccagca ccagctgaag gaggattcgg gtttgcggca tggggtttac
     7501 gtttttaacg gaatccttac caacgattac cttggtcggc tgtacaactt gccgtcgcgg
     7561 gacatcaacc tgctaatggc tgcattctag acggttttgc caatctactg ggccacaaat
     7621 aggtacgtaa tgctcccttt ctcctttcct gttgattgtt gggttgagga aaaacgacta
     7681 gccaacgcgg ccataatggc cgagttgtta atacactcgt cattcaccga gttcgcccag
     7741 tcaattgtgg catttgaaac gctgccatcg gacagcacct caatgtttac aaccacctgc
     7801 ccgcccattt cgcatttata ggaggggact ggtagcctgt atgctttccg gcctgccagg
     7861 ttatacgaga ttaccgttgg ccccttgtac atattttttt tctcctccgg tatatttttt
     7921 tcaggcgtat tgggaatggc tatgtcagct tctttcatag cctgctcata ccgctccttg
     7981 ttttcctgca tttgctccct tatgcggtta gcttcgctgt acaggttgtc ggcatccgtg
     8041 tttttttcgt ccgacaatcc tgcgtttagc tccttttggg tggcatctac cgcaaaattc
     8101 tttatagcct ccatttcacg actgggaacg gttacatcgg ttgggaggag ctgctctggc
     8161 ttttcttcaa ctagcaattc ctcctgcgcc ttgggttcct caaactccat ttcaatggtt
     8221 gatgcatatt cctccgttct atcctctatc ttgaaaacca agaatgacgc aatcaccaac
     8281 aggtgaataa ttatggtggt gagtatgccg actctatgct tagctattga agccgagaga
     8341 ctcttaaacc aactattcat tctactacca aacaattttt tagaattcat caggtggcta
     8401 atactgtttt gctttacaaa aattttagtt taactcctag ctgctttgaa aatgtttaac
     8461 gtaaagggat ttccgattta acaacagccg atctgttccc acaaccactc tcattgaatg
     8521 gggttatggt aaaatagtac gttttatccc tatccattcc gctaaaatag tattcattat
     8581 ggccataaac cattatgctt ccgtatagtt tgtcgggacg aactccaaaa ttaataaggt
     8641 atcccgttgc gttatcctga agtttccatt tcagccaggc attgcggggt tcggagttgc
     8701 ccctaagtac ggagaagaac tcaactgatt caggaagtgg caggttggct tttccaaaaa
     8761 cacgaaagcc gcttatggca aacttccctg ccgggacatg gaggttttcg agcttaagga
     8821 aacgaaattc tactggcgta cttagctcaa catagtcatg aggaacatcg gttttattct
     8881 ccgacttgtc aatcagtagc gaccagctct taccatcgag gctgccccag actctgtact
     8941 ggtggtaaat tcccatttgc ttgccaacaa gctgaacatc ctgatctgcg tagtttacct
     9001 gaaaggcgta tatgcggcag gtgtcgagca aatcggtaca aatccactca ccagcgttgc
     9061 ctgtagaagc gctccagtat gtgcgaatat cctcatcaac cgcaaaattg ggatagtaac
     9121 cgtagagggt gcttgaaaca gtaacgggtt tttgatagtt gagcagcatc cattcggtaa
     9181 attttccatt aaggtagtgg ggatagtcgc cgaaagcagt ttgagagtat agtgtaccat
     9241 cggcatcaaa gtatgccggc cacattccta ttctacgttc aaaattattt tttactccaa
     9301 tggcaattgt tgagatatgc caccattgct ggttgtaatc gcaaaaagtt gcaccatgtc
     9361 cggccccacg ggcaaaacca cctggtttgt agctaaaggg gttgaactcc tgatagcgaa
     9421 atggtccaag aggattgctg ccaatgtaaa cgccatcagc atacccgcta aattcggttc
     9481 caggcgccgc atactgaagg tagtaatgtc cgttatgctt gttcatccat gccccttcta
     9541 tgaatggggg taaaaaggta ttgtcgttgt actcgccaaa gcgttcccat ccatgaatac
     9601 tgtcgttaag gctaatcagg ggctttggag cagcttgagg gcgcagggtt gccctgttaa
     9661 cctcacagcc gtaaattggg taaaggttgc tggagccaaa ataaagatat actctgccat
     9721 cgtcatctgc aaagaaggcg ggatcccatg ctgctagtgg gaatgagtca attgcaatat
     9781 gccaatcgtc ggcggcgggg ttggtggatg cccaaatggg aaaatcgggg ttattcgaag
     9841 agccgattag caaaagggaa tcgcccagca caaaggccgc aggagcacag agatcgtcgt
     9901 aaaccttgtg tcgggggagt aaaaattttc tgggaataaa gttccagctg gccatgttgc
     9961 tactccacca gtagccatgc tgattggtag aaaataaaaa gaattttccc ttgaatgcca
    10021 ccaccagcgg gtctgctgta gccctatgtt ttacctgctc aattgctgct ggtattggac
    10081 tatagccgta atcaatatta attgggttgc agtaggtttt aagggtatcc tgcgaatagg
    10141 cttcagctgt gaagaattgc gtgagcgcta tagttaaccc tatcagtaaa ttcgatatgt
    10201 atgttacggt tgatattgag gtcgcctgga taatgggttt catgattctt gcaatttatg
    10261 gatgaaggac aacatgccgt ggtgcaatag caataagtat gtatcgggca aacaggctcg
    10321 ttatgttttg gatcaagtgc cgaaaaacct actgcgcaac ttggcatgtt tagattctca
    10381 aaattaaaaa tttattacga catgcccgca gagaggcaac atttatactt ttcagtaaaa
    10441 attgtggggg cattactttc agccgtttgc ccctttaaaa aatttgatac agctttgcag
    10501 cctaaatttt taagacaaat gctatcaatt cgtgaggttt ataaggaggg gtatggcccg
    10561 tcgagtagcc ataccattgg gccggtaaga gctgcagagc tattcataaa gaagcatcct
    10621 gaggtgagct acttccgggt aatgctattt gggagtttag cgctaacggg taggggtcac
    10681 caaaccgata gcgctttacg aaagtgtttt cttccaaaat cagtggaggt ggtgtttaag
    10741 cctgaggaaa cgctaccgct tcatcctaac gggatggtat ttgaggcgtt gaccgacgag
    10801 ggtgccgagg cgcttgaccg gtgggaggtt tacagcattg gcggcggtga tttaaaggat
    10861 aaaagcgggg tgttgaatag cagtgaggtt tattcgctca acaacatgac cgatattttg
    10921 acctggtgta ggagggaagg gaaaactttt tgggagtttg tggaggataa cgagggaaag
    10981 gatatatggg aatttctgca gggagtttgg cataccatga gggaaacgat aaaacggggt
    11041 attgaaaacg agggggtttt gcctggtccg ataaagcttg ccaggagggc ttcatcgcag
    11101 tattcgaaag cacttaccag tcatggaact ttaaaaagct ttggcctact gttttctttt
    11161 gccctggcag ttgccgagga aaacgctgca ggtggaagaa ttgtaactgc gccaacctgt
    11221 ggctcgtcgg gagttttacc agccatactg ttctacctga gccgggaaga gtaccttacc
    11281 gaggtaaaaa ttcatagggc gctggctact gcgggattaa ttggcaacct ggttaagcac
    11341 aatgcttcaa tttcaggagc agaagtgggc tgccagggag aaattggaac tgcttgcgca
    11401 atggctgccg ctgctatggt acaggttctt ggaggatctc caaaccagat tgagtatgcc
    11461 gccgagatgg gtatggagca taaccttggc ctaacctgcg atccggtgtt gggctacgtg
    11521 caaataccct gcatagagcg taacgccatt gctgcatcca aggcatactc ctgcggagtg
    11581 tacgcccttt cctctgatgg agggcataaa atttcgtttg accgggcagt tgaaaccatg
    11641 gccgaaaccg gccgtgatat tcaggtggct tacagggaaa ccggaattgg cggactggca
    11701 aaaaactggg aaccattcga ataggcaatg gtgcagtttt tttttcaaaa atggaaaggc
    11761 atcggaattg ctccctttgc cctgccattt cagcaaaacc tctattatca cttaaaccta
    11821 ttcgtgttgt tgcttgatgc tacttacgga ctatctcacc cgaacctaat atctttgcat
    11881 caacttgtgg gtttcctgag aggtaaatat cgccgctacc aacagagttg gcggtaaatt
    11941 tttccagtac gttggccgaa acatcacccg aaccggtaat ggctacgtcg gctaccctgg
    12001 taaccaggtt gcgggtatca atatcgcccg aaccgttgat tttgattacc agcgttgatg
    12061 cctccttttt cccacttacg gcaatatcgc ccgagccgtt tgtcttgagg gaaacttttt
    12121 cggcttccag ctcatttatt tttacatcgc cgctaccgtt aacctttacc tcaatttcgt
    12181 ttgttttgat gggggttttt gcaagcatat cgcccgaacc gtttatttca agcgttgaat
    12241 atttaggaga ccaaacgttt accaccacct cgtcatctgt gttgttgctg caactccaat
    12301 gattccatga ttggtccttc tccctgatgc tcagcagacc gttttctacc gaaatctcca
    12361 gcttgtcggc taccttctgg ctggttgtta tctcaacctt gtatgtggaa gcctggtaga
    12421 ggtttactgt agcggagatg ctattcttta cccctacaaa atccttgctg tcgttttgga
    12481 ttgtaacttg tccctgcgcg tacgcatagc tgcccattga agtcatcagg gcaaatgtgc
    12541 cggtaatcat aaatcttctc atggtttact ctttttagaa agatttttac ttcaaatcga
    12601 tgtatccctg taagtagcag gcttagcctt tggcttaaaa agaaaatcat ttttgctccg
    12661 tggaaaatac atttatcttt tcaatagact tctatccgat gcggaatgct gcatggaggg
    12721 tgattttttt ataaacctat atactgaggc agttagccat atctttcatg taaaacatga
    12781 gaatagtgtt accggcgttt agggtttacg gccttgttgt gttcaaaagt ctcttggtac
    12841 tccacgttgt tattcctgtc ataccgtatc caggaaccat gtttctggtt gtttttgaaa
    12901 tttccgattt gccaaagttg acccgactcc ctgtaaaaac gccattctcc ctccatcatg
    12961 ttgtcaggat atggcccttc agcctttact gtcccgtttt tgtagtaaaa aattagcatt
    13021 ccatcggcca acttgtaaac aggctgtccg ttagcgtaaa gttttttttc gagcattggt
    13081 gtaaactttt ttattgatga tggattcaag ttataaatgg aacttttaaa aagagaaaga
    13141 tgagtatagc gtaaacaaca ggctatcgga ttaatcgcca gaacttttac ccaccagctt
    13201 cagctgagtt caagttcatc gtgctaagtc aacctgtggt gggagaatta ataggcttcc
    13261 ccagttgtta atggttatcc ttttgctggt taagtttggt ttggtaggcg agtgcgtaca
    13321 tgcgcatctg cttaaccatg gccacaaggc cgttgctgcg ggtaggtgag aggttctccc
    13381 ttaatccaat tttgtcaatg aagtataggt cggcgttgat gatttcctcg ggagttctac
    13441 ccgagagtac cttgatgagc agcgacacta tccccttggt gattatggca tcgctgtcgg
    13501 ctgagaagaa aatccttccg tcacgcagct cggcatccag ccaaacctta ctctgacacc
    13561 cgtttatcag gtactggtcg gtgcgtttct tttcgtcaat tgggggcagc tccttaccca
    13621 gctctatgag ctgctggtag cgatccatcc aatcgttgga aatgctgaaa tcctcaacta
    13681 tggcatcctg aacctcatta agggtcattg tatgtggatt attttgttgt atgcgtgctt
    13741 gtaaacagaa cgaaatgctt ttagctgggt ttaatctcaa tcaggttgaa ctccaccttg
    13801 ttgatgagcg ccttgtagtc gttgcctgca aaaaatatgt cttcgtcatc ctcctcatag
    13861 taccagttga tggtaacttt cgtaccggtt tcaatgccaa tcttttcgaa ctttttcatc
    13921 aggtcaaaaa ttcgtttcga ggagctggtg ttgaagtact cgaatttcaa atggacggtg
    13981 gtttcgggtt gaatattttt accgtattcc tccacccatc caagaatggg ctggtagaaa
    14041 tcaataacat tttcgggaat cgacctgccc tccatcctta taaccccgct tgtagggtca
    14101 aagttgatgt agggggttgt tgcggtttcc ttgagctcga gttttagcat ctacaaaatt
    14161 tttattgggg caaatatgca aaaattatgc tagttggaaa attcctgaag tatctggtag
    14221 tcctcgccat gcccattccg tacaatagct tcccagtatc tatgggtaat tatagcttcg
    14281 ctaccgttgc ttttgttctt aaccctaatc cgttttgagc tatcggcgct ggtgttcacc
    14341 tctataaact caaacgggac gtgaagcatg gattcaaaat cccttccgag ctggtaaatc
    14401 gactcgtcgt cctgttcgta ccgccaggta acccgtacgc tggatgtttt gcgatggcac
    14461 tcctccagtt ttcgtagtag ctcgttgatg tacttattgg agctactgtt ggagtactcc
    14521 atgtaaataa tcacctcagt ttttggagac ggatgcttcg tgtaggtttc aacccattct
    14581 aaaagaggtt gccaaaaggt aaaggggtct tcggtaatgg accttccttc tatttccaga
    14641 atcccatcct ccgaaaaatt gatatacgga caatcggcac ttttttcaac tagtatgccc
    14701 atcgttattt acctgaattt cttaaagccc ataaagatat aagtttgttt ttaaatgtgc
    14761 aaaatttctt taaggaagct gtccatcact actaatgttt tgtggcagcg tgtatcctta
    14821 cgagttttcg tatgcttcaa taatgaaccg gtaaatgaaa aatgatgatt tcaccccttc
    14881 acgttgggag tattttcaat ggtgaaggtg gcgagaagtt gttttatatc aattagcttt
    14941 agcactaagg caaaaccaag agataccgca atggacaaaa catacgagaa agccatgccc
    15001 agatgcagat ttaaaatgga aacagtggcc agtggacaaa atagaaggta ggctaaaaag
    15061 cgaaaaacca tcgttctgtc aggagtgagg cgaaactttt tgagggaaat agtaacctgt
    15121 gcaacagcta ccaggaagtt tacgaccatg ttgccaattg ccgcaccagt taccccaaat
    15181 ctgggcacga gcgttatctg gacagaaagg caaacaacaa cggcagctcc tgaaacgatg
    15241 ttgagctcct tgatattccc attcgaggta agcagtgttc cgaacacgta acccactgaa
    15301 atgaagatgt aagaaatcat aagcagcccc agaaccggag cggataaatc aacatgttcg
    15361 tggtagagaa ggtttataat tggcgccctg tgggcaattg ttggaatcgc aatggctact
    15421 accggcacca ccagtagcag gaaggaaaaa ttaaggaaag ggccgaactc ccttttttgc
    15481 ttgatcatgc ctgagaaaat tgggagtaaa agtgaagcaa aaagaaatgg aagcatattg
    15541 gcggcatcga gcagcctgaa tgcctgagcg tatattcctg ccgcttcgct gccgttgggc
    15601 aacagccggt ctattaccac tgagtctaaa cggctgtagg ccgtcatcag caaaaccatc
    15661 agggcgtatg gcaagctatt ttttaacatg ctaagggata aaaccctgtc gaatttaatc
    15721 cgaagcctgc ccgttgtttg tgtaagaaat attagcgata ccgcaacggt tgccatgtac
    15781 gacaaggttt gagctacagc aaatgcttct atggtgaaat tctttctgag gggggtccaa
    15841 agaagcaccg agcccagcag aatcatgatg agcttatcca gcaccgacag cacgctatct
    15901 aggccaaaat gatgaagggc gttgaggttt gaccttataa acattaccag ggacgataaa
    15961 aactggttga ctgctagtat gaatagcagg ccaagttgat gatgcccata tcccataccc
    16021 agtgcaacag ccactgttat cgccacatac accacagaca gcattagctt gatggaaagg
    16081 atgttggcga agtacttacc ggcaagttgc ggatgaaagc ttatttccct gttgttgaag
    16141 ttggttaccc cgcaatcgag tagaatgttg aaaattaggg tgaagttgaa cagggcatag
    16201 tacaacccgt aatcacttgc ccccaaggca ttttgagccg acctgtctat gccaaaaatc
    16261 cagaacggct taacaatcag gttaagcaga agtagtaaaa acaggttggt tgaaaagaag
    16321 cggttggaaa cttcgggtat ttttttcaca tccggctacg ctttaaatga tacctaaacg
    16381 tgaatgcttt aaagaatatt ttgccaaaat gaataaatag gatagagccc ggcaatgttg
    16441 atgtggccga ggcctatctg gctataattc agctaccatt atgcttgcgt tcacttcata
    16501 ccgaaactat tattgccaac atatggctaa aagatagggt atgtagcata cttggtgctg
    16561 gcagcaggtt tcatctggga gattttcctg cacttgacct gctgatgcgt ggttatacct
    16621 ggcaggggtg ctgattaaac aagtttgcta cttttccttg ataaggaata tgtaaacggg
    16681 aaaatggtcg gagtagccac ccgagtaatt tgcacctaca taggttctga aagggtaccc
    16741 agcatattgc ccgctagttt gccttaaaaa tggtttgttg aacacctttg ccttataaaa
    16801 tacccagctg gttctgtcct tgctaagcaa gggctgtgtg attatgatat tatcaaaaag
    16861 attccaagag tcctggtagg caagagttcc aattccctca cggtaaagac tggccataac
    16921 gttgaaaagg tctcctgacc tcagcccttc agcgttgttt tttgccccaa ggtagtctat
    16981 gatactcctg ctgtcgggat cgtcgttcag gtcgcccatc aggataattt tggcatcagc
    17041 atcggttgct cgaattgagt caatgatgga gcgggagagc ttagcagctg caattcggta
    17101 tggctggcta cgtttttccc caccacttct cgatggccag tggttaacaa taatatgaat
    17161 ggtttcgcca tcaagcaagc cgctaacaac cagctgatcc ctggttctga attcagggtt
    17221 gtctggaatg ttcagcctga ccgatcggtg ggaggtaacc ttgaaaaact cggggcggta
    17281 tagcaaaccc acatcaacgc ctcgtctgtc gggcgagtcg tagtgaacaa tttggtagtt
    17341 cgattgtttt aacctaggag tgtttaccag gtcgtccagc accaaccggt tttcaatttc
    17401 gctgaccccg agaaccgctg gccctccggg gaacatctcg tcacctattt gtgctataac
    17461 ctctgccatg ttgttaagct tatggtagta acgttcggaa ttccacttgt tagggccatc
    17521 aggggtgaat tcaacatcat taggaccagg caacgtatca aaaagattct ccaggttgta
    17581 aaaacctatg cagcttacct tgtaggcttt cttttcctga gcctcggaat aacccaaagc
    17641 cacaagcatg agaaatactg ccagcacatt ccttagaaat aatctataca taatcaagtg
    17701 attttaggat aaacgaccga acaaagatag ctatatccgt aaaaaaagat ataatatttg
    17761 gcaactgtat aaaaaaatgt tctttttttg catcatataa ttccaacccg ttggttaggt
    17821 taaaatttct gaaatttgtt ggcttattaa ttttgttgtt caccccgata attgctgtgt
    17881 cagtgtaccg ccacctattt ttttgttgtt aatatttcta aaaataaaat gtagataagt
    17941 gaaacaattc atacatatca atcaacaacc aaataaataa caaattgtaa atgaagcgat
    18001 tattaacaac tgtgctgtcc ttgtttttgc tgggtatggg cagcttgtat gcgcagaata
    18061 ccctaagagg agttgtgaag gattccgaca cggatactcc tgttcaggat gtgagggtgc
    18121 gcttattagg cactacattt gaaacgttaa gcaatgctgg cggtgaattt acgttggagg
    18181 gtgttcccga tggcaaattc accttggagt tttccattgt aggctatcaa accattacgc
    18241 aggaggtaag ccttcccgag aattcgcaag tgcccgttgt ttcgctgaga cgcgaggtag
    18301 ttgccgataa cggatctcag gaatttagcg aggtggtgat atctgccgac gatctggatt
    18361 cggacaacat ggaccaggcg atttcggggt tgctacagtc gtcgcaggat gcctttaact
    18421 cggctgtttc gtacacattt agcccggcca gcttcaaaat ccgggggtat gattcggact
    18481 acagcttgct ctacatgaac ggagttccgg tgaacgaccc ggagattggt tttgcctcgt
    18541 ggagttcatg gggagggttg aacgatgttg tccggagcag ggagtcccgc aatggttttc
    18601 ttcctaccaa cttttcgttt ggcgggctgg gcggagcaac aaacctgaat gttcgtgcct
    18661 ctcagcagcg agttggaaca aggctttcct atgccgccac caaccgaacc tacaacaacc
    18721 gcattatgtt tacccactcc accggagtaa tggagaatgg cgtatcgttc accattagcg
    18781 gttctcgtcg gtgggcagaa gagggctatg tagatggaac caactacgat gcctgggcgt
    18841 actttattgg gattgaaaaa aagtttaaca gtcagcactc cgtagccttt actacctaca
    18901 acgctccaac caagaggggt atgcagggtg tttcgaccga ggaagcaaaa aatttagcag
    18961 gttccagcta ctacaaccca aactggggat accaaaacgg ggagaagcgc aactcacgag
    19021 ttcgctttca gcaggagcct acttttattc ttaaccatta ctggaacatg ttgcccaacc
    19081 tggcgcttac cactacggca ggctactcct ttggtaccta ccaaacaact gcgctaaact
    19141 ggtacgatgc ccaggatcct cgacccgatt actaccgcaa gcttccaagc tactggatcg
    19201 attccgatcc cgacgtgtct caagccattg ccgatgcatt tagcaacgat cctgctatca
    19261 gccagattga ctgggactac ctttaccagg tgaactacaa cagcctggac actgcctcca
    19321 accttctccg ctcaaagtac atcgtagaaa atagaattac cgattcccgt cagttctcgc
    19381 ttacctcggt ggccagctgg aatccaacgc aggagatgaa ggtgaacggc ggaattgaaa
    19441 tgacccttta taaaggccga aactacaaac aaattgacga cctgctgggt ggaaactact
    19501 ggctcgatat cgaccagttt gttgagcgcg actatttcga taacgccaac ctggcccaaa
    19561 acgatttgga caaccccaac cgtatggttg ccaagggtga tgtttttggg tacgactata
    19621 cggccaatgt aaataatgga aacctttggg ctgtggccaa ctacaccctg aaccgtttcg
    19681 agtactacct tggcggtagc tacacctaca ccgagttctg gcgtacgggg gatatgcgca
    19741 atggccgatt cccctctaac tcaaagggcg attctcccaa gcaacagttt aacgattttg
    19801 gggtaaaggg tggcgcaacc tggaaaattt caggccgtca ttacctcgat gccaacatgg
    19861 cttaccttac ccgtgcccca tttttcagaa attcctacat ctctccgcgc acatcaaact
    19921 ttgtaatacc aggattaggc agcgagaaga ttgccagcgg cgatatcaac tataacttgc
    19981 gaacacccta cattaaagct agaataaccg cctactacac ccgtttctat gaccaaacgg
    20041 agcttaaatc gttttacgac gactactacc gcaccttcat taactacacc atgcggaaca
    20101 ttgacaagga gcataagggt tttgaaattg gggctgaaat taaagcatcg cctaccctta
    20161 ctgtaagcac tgcagcagcg ctgggttcat accagtacaa atcaagaccc cttgccacca
    20221 ttacacagga caacctgggt tcggtgctgg ccgttgacca ggtggtgtat atcaaaaact
    20281 tttttgtccc caacacccca caaacggctg ccatggtagg cttaaggtat gcttcgccaa
    20341 aatactggtt cttgggagca acctttagct acttcgatga tatctacatt gattttgctc
    20401 cgggtaaacg tacagccgaa gccctggttg gccttgagcc caacgacccc cgtcgttacg
    20461 accttaccca tcaggaaagc gtttcgtcgg cctacctgct tgatgccaac attggaaaat
    20521 cgtggaggat taagaattac tacattaacc tgaactttca ggtggcaaat atcctcgaca
    20581 acaccgactt taaaacagga ggattcgaac aggcgcgcta ctccattgcc gagcaaacat
    20641 ccgataaatt tcctcctaaa tacttctacg cctacggtcg cacatactac ctgatttttg
    20701 gctttagatt ttaactcaac ttgtataata attatgatga gaacaatgaa attgactaaa
    20761 tactttgcag caacggtagt cctgattgcc atggtaacgc tatcgctgcc cggttgtgtt
    20821 gacttctcct tcgacgatcc ccagcccagc attgattcaa cctccatgtc ggccaacacc
    20881 accattgccg agcttaaatc ggtttatcct ggctctcttt tccagctctc cgacaccacc
    20941 ttctaccttc gcgattctat aatcatcgag ggaacggtga tttcggatga caaggagggt
    21001 aacttctaca agagcatttt tattgaggat agtacgggtg gaattgaggt taagctcaat
    21061 aaaacaaccc tgtataacga ctacaagcgc ggtcaaaaag ttgtggtgct ctgcaacgac
    21121 ctgtacctgg gtgactacgg tggtcaaatt cagctaggct caacctacaa aaacaatggg
    21181 gtagtagaaa taagcggtct ggagggcgat cctattatcc gcgagcatgt ttttaaaaag
    21241 gggaaaacca tacaggagcg tatccctacc gttttatccg ttacccagct caaccccacc
    21301 tacataggga tgctggttaa gttcgaaaat gttcaaattc gtgataccat ttctcccatt
    21361 acaagggaaa tatacaccta tgccgacaac gtgaacaagg taaccgttaa ccacagcatt
    21421 catcagtgtg ggcaggcatt tgacaaactt gtgctacgaa ccagcggcta tgcccgtttt
    21481 gcatccaaac ctattcaaac caaaaatggc tctattacag gagttcttac ctactataac
    21541 ggaacatacc aacttttgat tagggatacg aacgatgtgg attttacaag taatagatgc
    21601 agtaacaaat agaggaggaa ctaacgatga ttaaaaaaat actggcaatt ctggtgccca
    21661 tcgccatggt agggttaatt gcactacaaa atggctgtgt aaaggaggat ttcgataccg
    21721 ttcctccatt acgcaaggca atttcgtgga agggaactgc tacgatagct gatgtgaagg
    21781 gctttttcaa cgggccgaac aatccttccc gaacagctgg actgattaag aagctagtac
    21841 cagccagcta ctgggaacag cttgctgcca atggtgtagg cgattcctcc atcattattg
    21901 aagggctggt tatttcgtgc gattcggcag ccaactttta cgaaacggtt accatcatgg
    21961 actcaaccgg tggaattgat tttaaaatca atgccagcga cctgtacctc acctacggat
    22021 ttaaaccggg aaagcgggtg ctggttaagc tgaacgattt agccattgat agctaccatg
    22081 gggtttacca gataggatta ccctacaccg atgcaggcac acttaaggtt accggcgttg
    22141 accccagtat ggtggcagcc tgcacccaaa tgtctggtga gcaagctaac cttactccct
    22201 tgtcggtgga cattggcact attacagccg gctatgttca aaagctaatc gttattgata
    22261 gcgttcagtt ttggaatcct gaggctacat acagcttgcc gggtgttaat accaaccgca
    22321 ccctggtaga tacgaaagga aatcaaattg ttttgcgtaa tagcggctac tccaaatttg
    22381 taaacgacaa ggttccatcc gggagtggat atattgttgg ggtgctggga atatacgatg
    22441 gcacctacca gcttaccatt cgcgacccgc gcgacatcca gttcaacaat ccccgctttg
    22501 gctctcaacc acctgccaaa aatgccacca ttgctgattt aaaggccgct tgcacctcaa
    22561 acctggtgaa aattacacag gatttagtgg ttgaggcggt tgtactagcc aacgacaaat
    22621 ccgggaatat ttacaaaagt ctgtttattg aggacgaatc gggggcaata gagtttaaga
    22681 tagacgtgaa cgacctattt attgattttc cggttggaac aaaaattgcc atttcctgta
    22741 aggatatgta cgtaggtaag tatggcggtg tggtgcagct gggcggcgtt tacaacaatg
    22801 ccattggcag gatttccgcc aacgagtttt accagaaagt ctttgtggtt ggttcaggat
    22861 ttgaagtaac cccaattcct gtgagcatca acgaactaac agccgacctg gtaggcaaat
    22921 tagttaccat atctgatgtt cagtatatag attccgagtt gggcaaaaca tacgccgagt
    22981 cgagtatcac caatcgtcat gtggaggacg ctttggggag aaccgctatt gtgagaacaa
    23041 gtaactatgc tgattttgca ggtattaccc ttccaagcaa tagggggagt ataaccgccg
    23101 tgctgagcaa gtactacaac gactaccagc tttacattcg tgacctttcc gacgtttgga
    23161 tgacccaacc acgtcgggtt aaaaactaca ttcttaaaca ggactttagc tctgcaaccg
    23221 caaacagtcc aattagcgtt ggtggatggc aatccattgc ggaggttgga acacgcagat
    23281 ggatggccaa ggtttactcg ggcaacacct atgccgaaat gaatccatac cagtcgggcg
    23341 aagccagtaa tatcggatgg ctgatttccc ctgctattcc tgttgcttcg ggcgtaacaa
    23401 cctatcttac cttcaacacc caatttgcct actgggccaa ctccacgctc gaggtatttg
    23461 tgtcggaaaa ttacgatgga gctaaccctg cctcggcaac atggacaaaa ctttccaatg
    23521 ctcacatcgt aacgcagagc gaagggcaaa acacatgggt aagctccggc ctggttgata
    23581 tttcggagtt tcccggaact atttatctcg ggttcaagta ctctggagga ggttctattg
    23641 gccaaaccac cgcctttagg gttgatgacg ttaatgtttt tacacagcag taaaattatt
    23701 tagctgttac tttggtactc gaaggccaac cggattatcg gttggccttt actttaaccc
    23761 acaaattcta agataaagta taaagtggtt tcaagacttg tccccctccc ggcg
//
LOCUS       DAOSHM010000036        23194 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_1760_length_23194_cov_19.737932, whole genome shotgun
            sequence.
ACCESSION   DAOSHM010000036 DAOSHM010000000
VERSION     DAOSHM010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 23194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23194
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1760_length_23194_cov_19.737932"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(33..1016)
                     /locus_tag="PK839_06980"
     CDS             complement(33..1016)
                     /locus_tag="PK839_06980"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOG72513.1"
                     /translation="MHVKSSYYYLLVLPVLMLALFQVGCSTDDEQKNETLQNERIIRQ
                     AIEQVNPDSIRQYAVMLEAMGTRFALAPNRREVALAIMDKLKNFGYEDVQLDSFALNR
                     TWRNQYYETWQYNVVANLTGNVYPDSLVIIGAHHDCIVTSPSDNPMVATPGANDNASG
                     VAVTLEVARILILQDLKPSSTVRFVTFAAEELGLNGSGHMANQLHAAQAKVKMMLNND
                     MVSYFPSSEPSSWELNIIDYPTSTSLRLTAQNLACKYTLLSTTNDNTHQQSSDSYPFA
                     LKGYPAIFFIAHSDDPNYHTLNDSVSGCNFSFASEVAKLSCALILSKELHQ"
     gene            1240..4350
                     /locus_tag="PK839_06985"
     CDS             1240..4350
                     /locus_tag="PK839_06985"
                     /inference="COORDINATES: protein motif:HMM:NF022481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HOG72514.1"
                     /translation="MPKILPLGSNQQANTMSSYDTTQYKKFNNITGWAIFAIALVVYL
                     CTIEPTASFWDCGEFIASGYKLEVGHPPGAPFFMLLVRFFTMFAPSAQLVPVFANAMS
                     ALASAFTILFLFWSITHLARKIIGSESGSFSASQLILIFSAGAVGALTYTFSDTFWFS
                     AVEGEVYATSSLFTAIVFWAILKWEEAADEPYANRWIVLIAYLMGLSIGVHLLNLLAI
                     PAIVLVYYYKNYTYSHKGFFTALLLSAAILLIVMYGIIQGAVAIASGFELFLVNGLGL
                     PLMSGVVFFILTLIVLLVLGLRYTYRRRKYLLNTVLLGFTMILMGYSSYAVIVIRSSA
                     NPPMDQNSPDNMFSLKYYLNREQYGDRPLLYGQSFSAQPVDRTDGRLTYAPENGRYVV
                     VRKQSDYIYDPRFEMPFPRMYSSDPQHISAYKSWTNFKGRPVNVNTQDGKQQTIYTPT
                     FGENLKFFFSYQLGFMYFRYFMWNFAGRQNDIQGHGDVMKGNWICGIPFIDNARLGSQ
                     ELLPDTYKQNKGRNRYFMLPLILGLSGLIFQYRRRMKDFWVVMTLFVMTGIAIVVYLN
                     QTPYQPRERDYAYAGSFYAFSIWVGLGVMALYELTRRIKDGSATALATGAACTLMAPT
                     LMAFQNWDDHDRSGSYIPADFGYNMLVGCKPNSILFTYGDNDTFPLWYNQEVEGVRTD
                     VRVANLSYLRGDWYIEQMQRKVYESDPLPLRMTRKQYFSGKRDVVLVFDRIKEPINLN
                     MAVNFMLSDDPAAELKSPFDPGERINYIPSSNLFLPVDRQQVIKTQTVLPSYYDRIVD
                     TMRWKLNKNMVMKDGQVILDLLGTNSWVRPIYFGTTVNSDTYHGLNKYFHLEGLMYRI
                     LPVEAGEQWGVGSVNTSEMYGNLMGKYRYRGLNNPKVYIDENKFRIVSNYRNLYARLA
                     NALVDEGKTDSALVVLNHCMEVIPASTVPLNYYVLPIIEGYYRAGDKTSAIRISRLLM
                     DQSVKELDFILTQLNDRQQQNLFNSVQLDMAILQDLVRMAQSYEKGNHYEELNRTFNR
                     FLSMLQ"
     gene            4360..4995
                     /locus_tag="PK839_06990"
     CDS             4360..4995
                     /locus_tag="PK839_06990"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019152098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOG72515.1"
                     /translation="MNLSPPRFFQRFFKNIIWNFPDEKEGVFLTFDDGPTQGVTPWVL
                     DQLARYNAKATFFCLAKNVEMNPDIFERVVREGHAIGNHSYSHIKGWRTDTTQYIQDV
                     IYANSFLHTNLYRPPYGRITARQFRLITYQYKIVLWDVLSMDYSRRVSPKKVTKFVID
                     NVKPGSIVVFHDSLKAERNLRYALPRVLDAITATGMVCKPIVIPRKNIRES"
     gene            4950..6119
                     /locus_tag="PK839_06995"
     CDS             4950..6119
                     /locus_tag="PK839_06995"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOG72516.1"
                     /translation="MQANSYSKEKYPGELKKKGILFIVQLPPPVHGASMMNSMVYSRL
                     LGDLRYRCRLVRLNFARNLNDLQKFRFRKLGRSFTVFLEICWKALTFQPSIIYFSMVP
                     LGTTLLRDSAYLLAVRLLLPKSRKIIHLHRPGLYEFNQKYRLSWFYRFIFKGCEIIHL
                     TESLVEHEITPLQLTNVKVYAVPNSIEVDAAQHDAGVARMRYNILFLSNLFPHKGFIP
                     LVKAHALLKNNFPGLTLTIAGEIPNSDVEGRLRETIRELCLGDSVKVLGKVSGEQKSL
                     LYRQAGIFVLPSEQEYFPLVILEAMAARTPVITSGRHNLESLFTDMHDLLFVDDLSPE
                     GIAKTIQKLLDNPALASSIGENGYHRFCELQVQSQRMMEDLFNGCADYALLPTRP"
     gene            complement(6076..7284)
                     /locus_tag="PK839_07000"
     CDS             complement(6076..7284)
                     /locus_tag="PK839_07000"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOG72517.1"
                     /translation="MVRRIKKLPITIFTSETAPAFAGDGLNALLLAESLIKKGYSAQL
                     ICLNPNKLLPKSEVINGVTIRRIAYHYKNKRGRLWLRVNMLLYILGAARKQGYWLIYG
                     AMPANRLLVLTGWILGKKVFFRSTLYSFDDASTLISSKKNPVKAIYRFIYGRVAGYHA
                     LNSVFAEAWRKTYNEIIPIFVSPQGTTIPYPAPNIQNRKNARSELGLPPDPIIVLMVG
                     HLITRKGFPDIFEWLAKANCSFLLLHVGTIHAPESNIISIRNNEMRQNIVAGQNLLGE
                     RVKFMECSQRPSLYYYAADILLLASAAEGYPPNTVNEAMAAGLPILSNRIAGSTDYIS
                     DNHNGFLYTGFDEFISRFSQLVSNQDIRQQLGNNARLYAEKYLDVNPIAENLLKFMDE
                     SAARHSRHSH"
     gene            complement(7547..10606)
                     /locus_tag="PK839_07005"
     CDS             complement(7547..10606)
                     /locus_tag="PK839_07005"
                     /inference="COORDINATES: protein motif:HMM:NF016345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="basic secretory protein-like protein"
                     /protein_id="HOG72518.1"
                     /translation="MKRIVLRAVGIALLPLIWGSSNFVNAQYFGRNKPIYKNFKFKVY
                     QTPNFEIYHYLNNDSLLNHMAMSAEKWYDRHYQVFRDSIVKRNPILIYDNHADFQQTT
                     AISSTVGVGTGGVTEALKNRVIFPLTETWGQTNHVLGHEMVHAFQYNSLIHGDSTSLN
                     SVRNLPLWMVEGMAEYLSLGTYDYQTAMWMRDAVLNDNFPTLEDMSFKPNEYFPYRWG
                     HAFWSFIGKTFGDSLINPIFKETAKRGYDMALRRYIGLTEKSFSTLWKSVYYDYYKRI
                     LPDSSDNPSGNRILFERNAGEVNISPAVSPDGRYVAFYSEKDLFSIDLFVAHAISGKI
                     IRRLSRIVHNNEIDALNFIESAGTWSPDSRQFAFVAFSKGRNKLLVIDVYDDKLTSEM
                     YIEGVPSFNYPTWSPDGKSIVVSGLVNGVNDLYSMDIQTGRVTRLTNDPWCNIHPSWS
                     PDGSAIVYVTDKPSEIQAANKSQQVGYNLATLDVRTNNIKVYEVFPGADNLNPMFSGN
                     GHTIYFLSNSDGYRNLFKYDLNTGETYRLTRILTGISGMTPFAPAMSVAHENDEICYS
                     HFYNSKYSIYTADTTDFNPVKVDPYHVDFSASILPPLNRATEGLVDQNLTSNDLPEHL
                     VDSFKTVKFRPQFKLDYISNIGMGISTSPYGTGMAGGVEMLFSDITGAKMLYTGLSLN
                     GEVYDFGGQVSYINQRKYITWGVTLSHIPYSFGAYGYDTLHRVDNGNPIIIEDFQVLQ
                     YRMFETAGGGMAYFPLSQTQRFELTASVARYYYRVDIYSNYYVDGFYIGADRHKGDAP
                     PGFWMQQTNLAYVYDNSDFGIASPMRGNRVRLQVDKTLGDYNYWGSLVDLRKYIFAKP
                     FSFAFRGMYYGRYGTSIENSVLYPLYIGYPWYVRGYDSNVFIDYGQSGTTSIDQLTGD
                     QMVVTNFEVRVPFTGPERLTLIKSGAFYTELALFADAGIAWSKGYPPSLKWKPTSLND
                     RTPFVSAGLSIRINLFGMMVLEPYYAIPFQLGGFKSANWGINFLPGW"
     gene            10871..11152
                     /locus_tag="PK839_07010"
     CDS             10871..11152
                     /locus_tag="PK839_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72519.1"
                     /translation="MSKTINLNVMDEKLTILGIKIEDRMKEAGEVQKLLSRYGCSIKT
                     RLGLHEVAGDYCSTSGLIILELAGPAEDQRKLHDELQKIQGVLVRKMEF"
     gene            11317..12150
                     /gene="surE"
                     /locus_tag="PK839_07015"
     CDS             11317..12150
                     /gene="surE"
                     /locus_tag="PK839_07015"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019152093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HOG72520.1"
                     /translation="MQVEKGQDTNRVILVTNDDGYKSSGMEALIEMVKPLGRVVVVAP
                     EEGNSGMSHAITIKVPLRMKHRNRDDNVELYTVNGTPVDCVKLAMNELFAKIPPDLMV
                     SGINHGSNASISVVYSGTIAAAIEGCLYGIPSVGFSILDYGEYPDFRASILYGREILK
                     NVMEKGISPECCLNVNIPVLPPQEIKGVKVARQNRGSWREEFEKRTDPRGVNYYWLTG
                     YFHNDEPEATDTDEYFLSKGYITVVPVNVDFTDYREMKKLQGWENDLYNAVKNELSQA
                     K"
     gene            12128..12424
                     /locus_tag="PK839_07020"
     CDS             12128..12424
                     /locus_tag="PK839_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72521.1"
                     /translation="MNSAKLNRQRVGYLFGILLPLLVLVVIFFTMYTGKSFWAFLQIM
                     WIQGNLSQVLSLAVIPNLAIFFIFIWTKKLKTAQGVLGATFILAFLIIILRFAR"
     gene            12435..13559
                     /gene="lpxB"
                     /locus_tag="PK839_07025"
     CDS             12435..13559
                     /gene="lpxB"
                     /locus_tag="PK839_07025"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002559292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HOG72522.1"
                     /translation="MRYFIVAGEASGDLHASNLMRGIKAKDSSAEFRFFGGDLMKAQG
                     GTLVKHYRDMAFMGFLEVLLNLRTISGNMRLCKDEILAFKPDVLILVDYPGFNLRIAR
                     FAKKNEIRVFYYIAPKVWAWNESRVKVLKKYVDTLFIIFPFEQEYFARHGINAVYLGN
                     PTPDAIEQKIGGKVVTFNQFVEDNKLSQKPIIALLAGSRKQEIRYNLPVMLRMVEKFP
                     DYQLVIAGAPSLSEDVYRPYVAGKNVAIVFDQTYQLLSCSTLALVTSGTATLETALLN
                     VPQVVCYRGNILSMLVAWVVIRVKYISLVNLNMQAEVVKELKQFDLTPENLYAEASKL
                     LPGSPEREKMLTSYAQLQKQMGGSGASMRVGDKIVELISR"
     gene            13782..15026
                     /locus_tag="PK839_07030"
     CDS             13782..15026
                     /locus_tag="PK839_07030"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HOG72523.1"
                     /translation="MQKLTFTFIVSLILTLGAAGQKISISLFNNLKLSTILITPTSGS
                     YRLILGDNEVELKVNQILYISRSGDSLMVRDANQNMGTWKRVTVVGQTGEDIIRVKPI
                     VPASPARLYDDNLSFYVDLDRIFAINLIDQEKYVAAVVEAEGGLNREPEFYKVQALIV
                     RTYTFAHLNKHLAEGFNLCDEVHCQSYLGRSEKNSDILDAVLDTKDMVIVDAERNPIT
                     AAFHANCGGQTANSEDVWVKPLPYLVSVSDRYCLGSNSSTWERSIPIGEWKKFLSQNG
                     VDTLKIKKNSDLNFQAKTRPKYYAVMGVEIPTTDIRKHFRLRSAWFSVSAGKSEVRLS
                     GRGYGHGVGLCQDGAINMAQKGWTYDQIVQYYYKGVSIVKLTELDMDRIRVDTTLNNM
                     VEPNLPPPGMEFPQRPPDRGTR"
     gene            15062..15793
                     /gene="gldF"
                     /locus_tag="PK839_07035"
     CDS             15062..15793
                     /gene="gldF"
                     /locus_tag="PK839_07035"
                     /inference="COORDINATES: protein motif:HMM:TIGR03518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     permease subunit GldF"
                     /protein_id="HOG72524.1"
                     /translation="MFAIFTRELQNFFSSLSGYIAAMVFLVVTGLILWVFPGNLNILD
                     GGYASLNGYFSIAPWVLLLLVPAATMRTFSDEKRFGTLELILTRPISDGRIVLGKFFA
                     TLVLVLIVLVPSLVYYLSVYLLSSPIGNIDAGGTWGSFIGLFLLASTYASVGIFTSSL
                     TDNAIVSFLLSVLFCLFLFSGLSALASLPLFSGVKPLLMFLSISEHYASISRGVLDSR
                     DLVYFISMIFLFIIFTRTVLESRKW"
     gene            15818..17530
                     /gene="gldG"
                     /locus_tag="PK839_07040"
     CDS             15818..17530
                     /gene="gldG"
                     /locus_tag="PK839_07040"
                     /inference="COORDINATES: protein motif:HMM:TIGR03521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     substrate-binding protein GldG"
                     /protein_id="HOG72525.1"
                     /translation="MELKKSYKVKSLLRLLVSVVAVAALYALSSVLHFRIDLTSDKLY
                     TLQPITREILKNLNGEVSMKIYLDGDLPTGFIRMKRMLTETLDEFNVASRGKVRYELV
                     NPYSAKNSREQDRIFAGLYDKGLAPTSVQQRNRQGGSSQTIVFPGLLMSYRGKEMAVN
                     LLKNNPMLSAEENLNLSIQNFEFALVDALVKLSTDSLPRIAFIQGHGELDEFETGDIE
                     KSLSEYFRVDRVILNGDMEALKPYSVAIVAGPTKPFPEEDKLVVDQFIMRGGKVLWFV
                     DPVAVSLDSLSTGASTLAFVAQTNLDDMLFRYGVRLNPFIVQDLQCAVIPVNIAPSGQ
                     ASKFAPAPWPYFPLLIPPSNHPVTRNLNPILTRFVSPVDTVGENPLVKKHYLLYTSAT
                     SRILQVPLLINLSEIEQTPNEQHFINPNVPVAAELEGYFTSVFTNRPLKNYNHGEPFE
                     FRAKSSFTRMVVVADADMVRNDVQHRPNGAYVLPLGYDRYTQQTYGNKELVMNMVKYL
                     NDDNGIVNLRSRDFKLRLLDRSKASQSRLAWQVVNTVLPSLVLVLGGLIYFFIRRNHY
                     STPK"
     gene            17821..18951
                     /gene="dnaN"
                     /locus_tag="PK839_07045"
     CDS             17821..18951
                     /gene="dnaN"
                     /locus_tag="PK839_07045"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009000039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HOG72526.1"
                     /translation="MKFVVSSTELLSHLSVVSRVISSKNTLPILDNFLFNLEGNELEI
                     TASDLETTLVTRFSLENVSDEGTVAIPYKILIDTLKEFSEQPLTFDINPGTQNITINS
                     ENGQFHIVGQPADDFPQRAMLKPEDSHSVKLSAEILLNGINKTIFATADDEMRPMMNG
                     IFTELNEQGITFVSSDAHRLVRYRRKDVQVEADASFILPKKPAQLLKNMLVKETGFVN
                     VQFDDKNAVFTMPGYTMVCRLVEGNYPNYNAVIPVDNPNRMIVDRVDLYNSVRRVSIY
                     SNAASNMVKLSLTGNQCTVSAEDLDFSVSAFERLSCEYEGTEMEIGFKSIFLSEILAN
                     ISSTEVVLSFSEPSLASLVFPHINENPDEDILMLLMPMMIGD"
     gene            19127..19894
                     /locus_tag="PK839_07050"
     CDS             19127..19894
                     /locus_tag="PK839_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOG72527.1"
                     /translation="MKLNIKNPLAVIDLETTGIDIVHDRVVEIAVLKILPDGSENMRV
                     RRVNPGMPIPPEATAIHGITDEDVRNEPTFKEIARSLANHLEGCDFAGFNSNKFDLPL
                     LAEEFLRAGVDIDLKRRKFIDVQTIFHKMEKRTLGAAYKFYMDKELTDAHTAGADTMA
                     TYEILMAQLDRYPELKNDVNYLSEFSSFNRNVDFAGRIILDDRGVEVFNFGKHKGLPV
                     VEVLKKEPSYYSWMMNGDFPLYTKRVLTAIYLKMKQQ"
     gene            complement(19917..20864)
                     /locus_tag="PK839_07055"
     CDS             complement(19917..20864)
                     /locus_tag="PK839_07055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HOG72528.1"
                     /translation="MERLTEVEKEKILFIINPISGIRPKGNVEKNILKYLDNVKYRAE
                     FHVTTCKGDATAAVQAKLAEGYTKFVAVGGDGTVNEVASAIVGTEATLGIIPTGSGNG
                     LARHLRIPLNLKRAMAIINCGAADRIDYGMVNKIPFFCTCGVGFDAQIGYRFASNGKR
                     GFFNYIKIVISDFLRYKSKKYKLKIDGDGKFKIRAFLITVANSSQYGNNAYIAPLADI
                     RDGKLNLSIISPFKIFEAPVIGIRLFTKRIDKSTMEHSGAISEVVIKRKHKDVVHYDG
                     EPIIMGKKLKISVVPRGLRVIVPATKERIKHPFASRHEP"
     gene            21029..23173
                     /locus_tag="PK839_07060"
     CDS             21029..23173
                     /locus_tag="PK839_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011107669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HOG72529.1"
                     /translation="MSTTDSSRRVELISNRMGIKPRQVFNVIHLLEEGATIPFISRYR
                     KEATGNLDEVQIAEINSLSAKLLDLDRRKETILTTIEEQGKLTDSLRERIAGCYELSE
                     LEDIYLPYKPKRRTRATIAREKGLEPLALLVFNQVEKDILTKAQTFISDKVANVEEAL
                     QGARDIAAEMLSEDERVRNTVRRNFKQKAIIRSRVVKGKEAEGIKYSDYFEFEEPVRR
                     CASHRFLAIKRGEDEGFLRVSITPPADGVLDELRKSLLKSTGETAKQLELALADSYKR
                     LIEPSIENEFTAELKEKADAEAIRVFAENLRQLLLSPPLGQKNVLAIDPGYRTGCKMV
                     CLDAQGNLIHNETIYPHQPRNEQGKAMSKISSLVETFKIDAIAIGNGTAGRETESLVK
                     RIHFNRDVKVFVVSEDGASVYSASPVAREEFPDYDVTVRGAVSIGRRLMDPLAELVKI
                     DPKSIGVGQYQHDVDQSKLKEGLDRVVESCVNHVGVNLNTASMHLLTYVSGLGPQLAK
                     NIVDYRKQNGPFRSRTELKNVPRLGGKAFEQCAGFLRITEAQNPLDNSAVHPESYHIV
                     EQMAGDLECTVKDLIGNDQLRSRIRVEKYVSEKTGLPTLNDIMLELAKPGRDPRSTIK
                     VFEFAKDIFKIDDLTPGMVVPGIVTNITNFGAFVDIGVKQDGLIHISQLAGRFVSNPL
                     DVVKLHQHVRVRVLEVDIPRKRIQLSLKDVEQ"
BASE COUNT         5999 a         5109 c         5595 g         6491 t
ORIGIN      
        1 ttattttcta ttatccctat ttccgcttcc atttactggt gcagctcttt gctaagaatt
       61 agcgcgcagc taagcttggc cacctcgcta gcaaagctaa aattgcatcc cgacacggaa
      121 tcgtttaggg tgtggtagtt gggatcgtcg gagtgggcta taaagaaaat tgcagggtaa
      181 cctttaaggg caaaaggata ggaatcgctg ctttgctggt gggtgttatc gttggtggtg
      241 ctcagcaggg tatatttaca ggcaagattt tgcgcggtca accgtaggga ggttgaagta
      301 gggtagtcaa ttatgttcag ctcccatgag gagggttcgg atgaggggaa gtaggacacc
      361 atgtcgttgt tcagcatcat tttcaccttt gcctgtgccg catgaagctg gttggccatg
      421 tgcccgctgc cgttgagccc cagctcctca gcagcaaagg ttacgaagcg aactgttgac
      481 gagggtttta aatcctgaag tatcagaatt ctagcaacct caagcgtaac agccacgcca
      541 ctggcgttgt cgtttgctcc gggggttgcc accattgggt tatcgctggg gctcgtgact
      601 atgcagtcgt ggtgggcacc gattataacc aggctgtcgg gatagacgtt tccggtgagg
      661 ttggccacca cgttgtactg ccaggtttcg tagtactggt ttcgccaggt gcggtttagc
      721 gcaaacgagt cgagctggac atcttcgtag ccaaagtttt taagtttatc cataattgct
      781 aaggcaactt cacgccggtt aggggccagg gcgaagcgtg ttcccattgc ctccagcatt
      841 actgcatact gccttatact atcggggttt acctgctcaa tagcttgccg tataatcctt
      901 tcattctgaa gtgtttcatt tttctgttca tcgtctgttg agcagccaac ctgaaaaagg
      961 gctaacatga gaacaggtaa taccaacagg tagtagtagc ttgatttgac gtgcataggt
     1021 ggttggataa aaatggcttg aggatccaaa tttaataata gcaatccatt ttgcaccgga
     1081 ttggcatcat ttatggttaa ataattgtgt acctcgcgct ggttggttat taatagcctc
     1141 tattttaagg cttgcttttt tcaggaaccg catttgaata tagaaaatta tttacattac
     1201 agggtgctgg cgctccctta tgtttttatt ggttaatcaa tgccaaaaat tttacctttg
     1261 gggtcaaatc aacaggcaaa caccatgagc agctacgaca ctacccaata caaaaaattc
     1321 aacaatatta caggctgggc aatttttgcc atagcgcttg tggtttacct atgtaccata
     1381 gagcccacag cgagtttctg ggattgcggg gagttcatag ccagcggtta caagctggag
     1441 gtaggacacc cccccggtgc acccttcttc atgcttttag tgcgcttttt taccatgttt
     1501 gcaccttcgg cacagctggt tcctgtattt gccaacgcca tgtcggcgct agcaagcgct
     1561 tttaccattc tattcctatt ttggagcatt acccatttgg caagaaaaat tattggttcc
     1621 gaaagtggaa gttttagtgc cagccagctg attcttattt tttcggcagg agcagtagga
     1681 gcgctgacct ataccttttc cgataccttt tggttttcgg ctgttgaggg ggaggtttac
     1741 gccacctcat cgttgtttac cgccattgta ttttgggcta ttctaaagtg ggaggaggct
     1801 gccgatgagc cctacgccaa ccgatggatt gttctgatag cctatcttat gggtttgtct
     1861 attggcgtgc atctccttaa cctgctcgca attcctgcaa ttgtgctggt ttactactac
     1921 aagaactaca cctactcgca taaaggtttt tttacagcgc tgctgctatc ggcggctatt
     1981 ctgctcattg taatgtatgg catcattcag ggggcggtgg ccatcgcttc aggatttgaa
     2041 cttttccttg ttaatgggct gggactaccc ctgatgtcgg gagttgtttt cttcattcta
     2101 accttgattg ttctgctggt gcttgggcta cgctacacct accgccgaag gaagtacctg
     2161 ctgaatactg ttttgctagg tttcaccatg attcttatgg gttacagctc ttatgcggtt
     2221 attgtaatcc ggtcatcggc taaccctcca atggaccaga atagtccgga taacatgttc
     2281 tcgttaaagt actacctcaa ccgcgaacag tacggcgata ggccgcttct atacggtcaa
     2341 tcgttcagcg cccaaccggt tgatcgcacc gatgggaggt taacctatgc ccccgagaat
     2401 gggcggtacg ttgttgtcag gaagcaatcc gactacattt acgatccccg ttttgagatg
     2461 cctttcccgc gcatgtacag cagcgatccg cagcacatca gcgcctataa aagttggacc
     2521 aactttaagg gtaggccggt taatgttaac acccaggatg gtaagcagca aaccatctat
     2581 actcctactt ttggcgagaa tctgaaattt ttcttcagct accagctggg tttcatgtac
     2641 ttccggtact tcatgtggaa ctttgccgga cggcaaaatg acattcaggg ccatggcgac
     2701 gtgatgaagg gcaactggat ttgcgggatt ccttttattg ataacgccag acttggttcg
     2761 caggagttac tccccgacac ctacaagcag aacaagggac ggaacaggta tttcatgctt
     2821 ccgctcattc tcggattatc agggttgata ttccagtaca ggcgtaggat gaaggatttc
     2881 tgggtggtga tgacgttgtt tgtaatgacc ggcatagcta ttgtggttta cctaaaccaa
     2941 accccatacc agcctcgaga aagagattat gcctatgctg gtagctttta cgctttcagc
     3001 atttgggttg ggttaggtgt gatggcccta tacgagctta ctcgtagaat taaggatggt
     3061 tcagccaccg ctttagccac gggcgctgct tgcaccctga tggcgcctac cctgatggcc
     3121 ttccagaact gggacgatca cgaccgatcg ggaagctata tacctgccga ttttggatac
     3181 aacatgctgg ttggctgcaa gccaaactcc atcctgttta cctatggcga taacgatacc
     3241 tttcccttgt ggtataacca ggaggtggag ggcgtacgaa ccgatgtgag ggttgccaac
     3301 ctcagctacc ttcggggtga ctggtatatt gagcagatgc agcgcaaagt ttatgagagc
     3361 gatccccttc cgctgagaat gaccaggaaa cagtacttct ccgggaaacg agatgtggtg
     3421 ctggttttcg acagaattaa ggagccaatc aacctgaaca tggcggtaaa cttcatgctt
     3481 agcgacgatc ccgctgctga gctgaaatcg ccattcgatc cgggcgagag gattaactat
     3541 attccctcca gcaacctgtt tttgccggtt gacaggcagc aggttataaa aacccaaacc
     3601 gttttgccct catactacga cagaattgtt gataccatgc ggtggaagct gaataaaaat
     3661 atggtgatga aggacgggca ggtaattctc gacctgctgg gaactaatag ctgggtgcgc
     3721 ccaatttact ttggaactac ggtgaatagc gatacatacc acgggcttaa taagtatttc
     3781 caccttgaag gattgatgta caggatttta cctgttgagg caggagaaca gtggggtgtg
     3841 ggcagcgtaa acacctccga aatgtacggc aacctgatgg gtaagtacag gtatagaggg
     3901 ctgaacaacc cgaaagttta cattgatgag aacaaattcc gaattgtctc caactatagg
     3961 aatctttatg cccgcctggc caacgcattg gtggatgagg gcaaaaccga ctcggctttg
     4021 gttgtgctaa atcattgcat ggaggttatt ccggcaagca cggtgccgtt gaactactac
     4081 gtgctgccaa ttatcgaggg ttattacagg gcaggagata aaacttcggc cattagaata
     4141 tccaggttgc taatggacca atcggtaaaa gagcttgatt tcatacttac ccagcttaat
     4201 gataggcagc aacagaacct cttcaacagt gtgcagctgg atatggccat actgcaggac
     4261 ttggttcgca tggcccagag ttacgagaag ggtaatcatt acgaagagct gaatagaaca
     4321 tttaaccgtt ttctgtcgat gttacagtaa atcaacatga ttaaccttag tcccccccgt
     4381 ttctttcagc gtttttttaa aaatattatt tggaattttc ctgatgaaaa ggagggggtc
     4441 tttttaacat ttgatgatgg gcctactcaa ggggttaccc cctgggtact cgaccagctt
     4501 gcccgctaca acgctaaggc tacatttttt tgtctggcta aaaacgtgga gatgaatccc
     4561 gatatctttg aacgagttgt tcgtgagggg catgctattg ggaaccactc ctatagccat
     4621 ataaagggtt ggagaaccga tacaacccag tacattcagg atgtcattta cgccaacagc
     4681 ttcctgcata ccaacctcta ccggccgcct tacggcagaa ttactgcacg gcagtttaga
     4741 cttatcacct atcagtacaa aatagtgctt tgggatgtgc tcagcatgga ctacagccgg
     4801 agggtttcgc caaaaaaggt tacaaaattt gtcatagaca atgtgaagcc aggttcaata
     4861 gtggttttcc acgattctct taaggctgaa aggaatctga ggtatgcctt gccacgggtt
     4921 ttggatgcca ttactgcaac cgggatggta tgcaagccaa tagttattcc aaggaaaaat
     4981 atccgggaga gttgaaaaaa aagggaatac tattcatcgt tcagctgcca ccacctgttc
     5041 acggagcaag catgatgaac agcatggtgt acagcaggtt gttgggtgac ctaaggtatc
     5101 gctgtaggtt ggtcaggtta aattttgcta gaaatctgaa cgatctgcag aagtttcggt
     5161 ttcggaagtt aggtcgttct tttactgttt ttctggagat atgctggaag gcattaacct
     5221 ttcaaccttc cattatatac ttctcgatgg ttccgctcgg aactacactt cttcgcgata
     5281 gcgcatactt gctggctgtt aggcttttac ttcctaagtc gagaaaaatt attcaccttc
     5341 accgtcccgg actctacgag tttaatcaga aatacaggct ttcatggttc tataggttta
     5401 tatttaaagg atgtgaaata atacacctta ccgaatcact ggtagaacat gagattacgc
     5461 cacttcaact aacaaatgta aaggtgtatg ctgtgcccaa ctccattgag gttgatgcag
     5521 cacagcatga tgccggcgtt gccaggatga ggtataatat cctgtttctc tcaaatcttt
     5581 ttcctcataa gggttttatt ccgctggtga aggcccatgc cttgttgaag aacaattttc
     5641 cggggctgac gcttactatt gccggagaga ttcccaattc cgacgttgag ggtagattgc
     5701 gggaaacaat ccgtgagctt tgccttggcg atagcgtgaa agttctggga aaggtttccg
     5761 gtgagcaaaa atcattactt taccgtcagg caggcatttt tgtgctgccc tccgaacagg
     5821 agtatttccc gctggtaatt cttgaggcta tggctgcccg aaccccagtg attacaagtg
     5881 gtaggcataa cctggaatcg ctgtttaccg atatgcacga cctgctgttt gttgatgatc
     5941 tttcacccga aggcattgct aaaacaattc aaaagctgtt ggacaaccca gcacttgcca
     6001 gtagtattgg cgaaaatggt taccaccgtt tttgtgagct ccaagtgcag agccaaagaa
     6061 tgatggaaga tttattcaat ggctgtgccg actatgcctt gctgccgact cgtccatgaa
     6121 tttaagtagg ttttcggcta tgggattgac atcaagatac ttttcggcat acagccgggc
     6181 gttattgccg agctgctggc gaatatcctg attggagacc agctggctga acctggaaat
     6241 gaattcatca aaccccgtgt acagaaaacc attgtggtta tcggaaatgt agtcggttga
     6301 gcctgcaatc ctgtttgaga gaattggcaa tcctgctgcc atggcttcgt ttaccgtatt
     6361 tggggggtag ccttctgcag ccgaggctag aagtaatata tccgcagcat agtagtagag
     6421 tgaggggcgc tggctgcatt ccataaactt aaccctttca cccagcaggt tttgccctgc
     6481 tactatgttc tgcctcattt cgttgttgcg gattgagata atgttcgact ccggggcatg
     6541 aatggttccc acgtgtagga gcagaaagct acaattcgcc tttgccaacc attcgaatat
     6601 gtcgggaaaa ccctttcggg ttatcaggtg tccgaccatt agtacaatta tcggatcagg
     6661 gggtaagccc agctcactcc gggcattctt tctgttttga atgtttggtg caggatatgg
     6721 tatggtggtt ccctggggcg atacaaaaat cggaatgatt tcgttgtagg tttttctcca
     6781 tgcctctgca aagacgctgt ttagcgcatg gtatcctgct accctaccat aaatgaacct
     6841 gtagatggct ttgacaggat ttttcttgct cgaaattagc gtggaggcat cgtcaaaaga
     6901 gtagagggtt gaccggaaaa aaaccttttt cccaagtatc caaccggtga gtactagcag
     6961 acggtttgcc ggcattgctc cgtatataag ccagtagcct tgctttctgg cagcaccaag
     7021 aatgtaaaga agcatgttca ctcttagcca taatctacct cttttgtttt tgtaatggta
     7081 ggctattctt ctaatggtaa ccccattgat aacctctgat ttaggtagaa gcttgttagg
     7141 gtttaggcag attagctgcg cactgtaccc tttttttatc agggattcag ccagaagtag
     7201 ggcattcagc ccatcaccgg caaatgcggg tgcagtttcg gaggtaaaga tggtaatggg
     7261 caacttcttt attcttcgca ccattgctag aggctggtgt taaggtagat tttttggttt
     7321 gaaaaaagtg taggcaataa aagtggatac cttacttgtt gaatgcgtta aatatttatt
     7381 ctaatacaac aattttttca tactatcagt atcgttcgtg tcctatcgaa tttaatggta
     7441 cacggaaaac acagattaaa ctgatttgaa ctgattaatc tgtgattttc cgtcgcatcc
     7501 gcgtcatccg agtcatactt ttttatttat gagaaaaaaa gaatcgctac catcccggca
     7561 ggaagttgat accccagttg gccgatttga atccccccag ctgaaatgga atggcgtagt
     7621 agggctcaag caccatcatc ccaaacaggt ttattcgtat gcttaggccg gcgctaacaa
     7681 agggagtgcg gtcgttgagg gaggtaggct tccactttag gctgggtggg taccctttgc
     7741 tccacgctat gccggcatcg gcaaataggg caagttcggt ataaaatgcc ccggatttaa
     7801 taagggttaa cctttcggga ccggtaaacg gcactcttac ctcgaaattt gtaaccacca
     7861 tttggtcgcc ggttagctgg tcaatgctgg tggttcccga ctgcccgtag tcaataaaaa
     7921 cattgctgtc gtaacccctc acgtaccatg ggtagccaat gtaaagcggg tatagaacgc
     7981 tgttttcaat agatgttccg tatcttccgt agtacatccc tctgaatgca aagctgaagg
     8041 gtttggcaaa tatgtatttc ctcaggtcaa ccaggctacc ccaatagttg taatcgccca
     8101 gggttttgtc aacctgaagc ctgacacggt ttcccctcat gggcgaggct atgccaaaat
     8161 cggagttgtc gtaaacgtaa gccaggttgg tttgttgcat ccagaaaccc ggaggagcat
     8221 cgcccttgtg cctgtcggct cctatgtaga agccatcaac atagtagttt gagtagatgt
     8281 caaccctgta gtagtaacgt gctaccgaag cagtgagttc aaatctttga gtttgcgaaa
     8341 gcgggaagta ggccatacct cctcctgccg tttcaaacat gcggtactgt aacacctgaa
     8401 aatcctctat gattatcggg ttaccattat caactcgatg cagggtatcg tagccataag
     8461 ctccaaagga gtaagggata tgcgaaaggg taacacccca ggttatgtat tttctttggt
     8521 tgatgtatga cacctgtcca ccgaagtcat aaacctcgcc atttaatgat aggccggtgt
     8581 agagcatctt ggcgccggtg atatcgctga agagcatttc cacgccaccc gccattcctg
     8641 ttccataggg cgaagtgcta atgcccattc caatgttgga gatgtagtcg agcttgaact
     8701 ggggtctaaa ctttaccgtt ttaaatgagt caaccaggtg ttcaggaaga tcattgcttg
     8761 ttaggttttg gtcaacaagc ccttcggtgg cccggttaag tggtggtaga attgaggcgc
     8821 tgaagtcaac atggtacgga tcaaccttca ccgggttaaa gtcggtggta tcggcggtgt
     8881 agatggagta cttgctgttg taaaaatggg agtagcagat ttcatcgttt tcgtgggcca
     8941 cgctcatggc aggtgcaaag ggggtcatcc ccgatattcc agttaggatt ctcgtcaggc
     9001 ggtaggtttc gcccgtattg aggtcatact tgaagaggtt gcggtagccg tcgctgttgg
     9061 agaggaagta gatggtgtgg ccgttacccg agaacatggg atttaggttg tcggctcctg
     9121 gaaatacctc gtaaacctta atgttgttag ttctcacatc aagtgttgcc aggttgtagc
     9181 ccacctgctg gcttttgttg gctgcctgaa tttctgaggg tttatcggta acataaacaa
     9241 tggctgagcc atcgggcgac cacgatgggt gtatgttgca ccaagggtcg ttggtcaacc
     9301 gggttaccct tccggtttga atatccatgc tgtagaggtc gttaacgccg tttaccagcc
     9361 ccgagaccac tatagacttt ccgtctggag accaagttgg gtagttgaac gatgggacac
     9421 cttcgatgta catctcgctg gtgagcttat cgtcgtaaac gtcaatgacc agcaacttgt
     9481 ttctgccctt gctaaaggcc acaaaggcaa actgccggct atcgggcgac catgttcctg
     9541 ccgattcaat gaagttgagg gcatcaatct cgttgttgtg gactatgcgc gacaggcggc
     9601 ggattatttt tccgcttatg gcatgggcta cgaataggtc aatcgaaaac aaatcttttt
     9661 cggagtaaaa agctacgtat cggccatcgg ggctaacagc tggcgaaatg ttgacttctc
     9721 cggcattcct ttcgaataga atgcggttgc ccgacgggtt gtcggagctg tcggggagaa
     9781 ttctcttgta gtagtcgtag taaaccgatt tccacagggt agagaaggat ttttcggtga
     9841 gcccgatgta ccttcgaagg gccatatcat agcccctttt ggcagtttcc ttgaaaatcg
     9901 ggttgatgag gctgtcgcca aaggtttttc caatgaacga ccaaaacgca tgaccccaac
     9961 ggtatggaaa atactcgttg ggtttgaacg acatatcctc aagggtggga aagttgtcgt
    10021 tgagcactgc atcgcgcatc cacatggctg tttggtagtc ataggttccc agcgaaaggt
    10081 attcggccat gccttcaacc atccatagcg ggagattacg caccgagttt agcgaggttg
    10141 aatcaccgtg tatgagggag ttgtactgaa aagcatgaac catttcgtgg ccaagtacgt
    10201 ggttggtttg cccccaagtt tcggtgaggg gaaaaatcac ccggttttta agggcctcgg
    10261 ttaccccgcc ggtaccaaca ccaactgtac tcgaaatggc tgtagtttgc tgaaaatcag
    10321 catggttgtc gtagattaaa atgggatttc gttttacgat ggaatccctg aaaacctggt
    10381 agtgcctatc ataccatttc tcagctgaca tggccatgtg gttgagcagg ctatcgttgt
    10441 tcaggtaatg gtatatttca aagttgggcg tttggtaaac cttaaacttg aaattcttgt
    10501 atattggctt gttccggcca aaatactgag cattaacaaa gttcgatgat ccccagatta
    10561 gaggcaagag agcaattcca acagctcgta gtactatcct tttcatgcga ttatccattt
    10621 ttctgtccgc atcatagctt gtaaccagta tcgcaaaaaa atgttgtttt caacaatttg
    10681 aaatacagta tcccaaaccg gttaaaaatt aaacctacag tagccaaata taagaacctg
    10741 taagctgtga aaacatgcaa atttcaattc tcgaaaaata gctgtttctt atcaaaaatc
    10801 acaccagatc agcttttttt gtatgaatta gacggggata ttttttagca tgggaatatt
    10861 ttgtaaattt atgagcaaaa ccattaacct taacgttatg gatgagaagc taactatttt
    10921 gggaattaaa attgaggaca ggatgaagga ggccggcgaa gttcagaaat tactttcgcg
    10981 ttacggttgc tccatcaaaa cccggctcgg attgcatgaa gttgccggtg attactgcag
    11041 cacttcgggt ttaattattt tggagctggc cggaccggct gaagatcaga gaaaactcca
    11101 cgacgagcta cagaaaattc aaggagtgct tgtccgcaaa atggaattct gatttcagct
    11161 gtaaaacact ttgctttagg ttgagttgaa acgaatctgc tttcgtttca actcttttat
    11221 attcctgccc cattatgggg agcattaaga ataactgaat ctgaagtggt taaaaaatag
    11281 tatctttgca ggctaatatt ttgagcgaat aagataatgc aagtagagaa agggcaggat
    11341 acaaatcggg taatactggt aaccaacgac gatgggtaca aatcgagcgg aatggaagca
    11401 cttatcgaga tggttaaacc gctgggaagg gtagtagtgg tggctcccga ggagggtaac
    11461 tcgggaatgt cgcatgctat caccattaag gttccgttga ggatgaagca tcgcaaccgc
    11521 gacgataatg ttgagcttta caccgtaaac ggcacgccgg ttgactgcgt gaaacttgct
    11581 atgaacgagt tgtttgcaaa aatacctccc gatttgatgg tttcaggcat taaccatggt
    11641 tcaaatgcct ccattagcgt ggtttacagt ggaacaattg ctgcggccat tgagggttgc
    11701 ctttacggca taccatccgt gggtttttca attcttgact acggggaata ccccgatttt
    11761 agggcatcaa ttctatacgg acgtgaaatt ctaaagaatg taatggagaa gggtatttcg
    11821 ccggaatgct gcctgaatgt taacataccc gtgcttcctc cccaggagat aaaaggggta
    11881 aaagtagccc ggcagaatag gggcagctgg agggaggagt ttgagaaacg caccgacccc
    11941 aggggagtta actactactg gctcactggc tactttcaca acgatgagcc cgaggcaacc
    12001 gataccgacg agtacttcct ttctaagggt tacattactg ttgtgcctgt taacgtcgat
    12061 tttacagatt accgggagat gaaaaagctt caagggtggg agaatgacct ttacaatgcc
    12121 gtaaaaaatg aactcagcca agctaaatag acaacgggta ggttacctct tcgggatttt
    12181 gctacccctg ctggtgctgg tggtgatttt ctttactatg tacaccggta aaagcttctg
    12241 ggcttttctc cagattatgt ggattcaagg caacctatcg caggtgctgt cgcttgctgt
    12301 tattcccaac ctggctattt tcttcatatt catttggaca aaaaaattga agactgccca
    12361 gggtgtgctg ggggcaacat ttatacttgc atttctcatc attattttac gttttgcacg
    12421 ataaaaccag ctaaatgcgc tactttatag ttgcaggaga agcatcgggc gatttgcacg
    12481 cctccaacct gatgcgggga attaaggcca aagattcttc ggccgaattc agattctttg
    12541 gtggcgattt gatgaaggct cagggaggaa cactggtaaa gcattaccgc gatatggcct
    12601 tcatgggctt cctggaggta ctgctgaatc ttcgcactat ctccggcaac atgaggcttt
    12661 gtaaggatga gattcttgcc tttaagcccg atgtgcttat actggttgat taccccgggt
    12721 ttaaccttcg gatagcacgc tttgcaaaaa agaacgaaat acgggtattc tactacattg
    12781 cccccaaggt gtgggcatgg aacgaatcgc gcgttaaagt tctaaaaaaa tacgtagata
    12841 cgctcttcat tatttttcct tttgagcagg agtattttgc cagacacgga ataaatgcag
    12901 tttacctggg caacccaaca cccgatgcca ttgagcagaa aattggcggt aaggttgtaa
    12961 ccttcaacca gtttgttgag gataataagc tatctcaaaa gcccataatt gccctacttg
    13021 ccggaagtcg taagcaggaa ataaggtata acctaccggt tatgctcaga atggtggaga
    13081 agtttcccga ctaccagctt gtgatagccg gagcaccctc gttaagcgag gatgtatatc
    13141 ggccctacgt tgcaggaaaa aacgtagcca ttgttttcga ccagacctac cagctgttaa
    13201 gctgctctac cctggcgctg gttacttcgg gtactgcaac cctcgaaacc gctctgctca
    13261 acgtgcctca ggtggtttgc taccggggca acattctttc gatgcttgta gcctgggtgg
    13321 ttattcgggt aaagtacatt tcgttggtta acctgaacat gcaggctgag gtagtaaagg
    13381 agcttaagca gttcgattta actcccgaaa atctttacgc cgaagctagc aagctgctgc
    13441 cgggtagccc cgaaagggaa aaaatgctga ccagctatgc tcagcttcaa aagcaaatgg
    13501 ggggtagtgg cgcttccatg cgggttggtg acaagatagt tgagcttatt tcacgctgat
    13561 tttattgctc atcttgttca ttgatagcaa atctcccccc tacagcaatt ctttaacaga
    13621 atttagttgt tgccagcgcg ctgtttttgt aagcaggtga atcaatattt tacgttttac
    13681 ctatattgca ccccaatttg ggaaatgaaa tagtacatgc tacttaaagc cttaggttgc
    13741 attaacagat acggatgtaa gtcaaatttc aacagtattt tatgcaaaag ctaactttta
    13801 cgtttatagt atcgttgatc cttacgttgg gtgctgcagg acaaaagatt agcattagcc
    13861 tgttcaacaa ccttaagcta tcaaccattt tgattacgcc aaccagcggc agctaccgct
    13921 taattctggg tgataatgag gttgaactaa aggtgaacca aatactttac attagtcgtt
    13981 ctggagattc cctcatggta agggatgcta accaaaacat gggaacatgg aagagggtta
    14041 ccgtggttgg ccaaaccggt gaggacatta ttcgagtaaa accaattgta cctgcaagtc
    14101 cggctcgttt gtacgacgat aacctgagtt tctatgttga cctcgaccgt atttttgcca
    14161 tcaacctaat tgaccaggaa aagtatgtgg cagctgttgt agaagctgaa ggaggactta
    14221 atcgggaacc ggagttttat aaggttcagg cgttaattgt tcgaacttac acctttgccc
    14281 acctaaataa acacctggcc gagggtttta acctatgcga tgaagttcac tgccagtcat
    14341 acctaggtcg aagcgagaag aatagtgata ttctggatgc cgtacttgat actaaggata
    14401 tggtaattgt tgacgccgaa aggaatccaa ttactgctgc attccatgct aattgtggag
    14461 gacaaactgc caactccgag gacgtatggg taaaaccctt gccatacctg gtttctgtgt
    14521 ccgacaggta ctgcctgggt tcgaatagct caacatggga gcgcagcatt cccatcgggg
    14581 agtggaagaa gttcctttca caaaatgggg tggacaccct taaaattaaa aagaattccg
    14641 atttgaattt tcaggccaaa acccgaccaa aatactatgc tgtcatgggg gttgaaattc
    14701 ctaccactga cattcgtaag catttcaggt tgcgttcggc atggttttcg gtttcagctg
    14761 gtaagagcga ggttcgcctt tcgggtcgtg gctacgggca tggggttggc ctttgccagg
    14821 atggtgctat aaatatggct caaaagggtt ggacttacga tcagattgtc caatactact
    14881 ataagggtgt tagcattgtg aagcttacgg agcttgatat ggataggata agggttgaca
    14941 caacccttaa caatatggtg gagcccaacc taccaccccc tggaatggaa tttccacaac
    15001 gacctcccga caggggaacc aggtagtgtt tgattaaatt ttataaaaaa accaaactac
    15061 catgtttgct atttttacca gggaacttca gaactttttc tcctcgctgt cgggctacat
    15121 tgccgcaatg gtttttttgg tggttacagg cttaatcctt tgggtgtttc cgggcaacct
    15181 aaacattctt gatggtggtt atgcctcgct aaacggatat ttttccatag ctccctgggt
    15241 actactgctg cttgttccgg ctgctaccat gcgaactttc tccgatgaga agcgttttgg
    15301 aactttggaa ttaattctca cccgtccaat ttccgacggc cggattgttt taggtaaatt
    15361 ctttgccacg ctggtgctgg tcctgattgt tctggttcca tcgctggttt actacctttc
    15421 ggtttacctg ctttcaagcc ctattggaaa cattgatgcc ggtggaacct ggggatcatt
    15481 catcggactc tttcttctgg cctcaaccta cgcttcggtt ggtattttta cctcaagcct
    15541 caccgacaat gccattgttt cattcctgtt gtccgtgcta ttttgccttt tcctattttc
    15601 gggcttaagc gctcttgctt cgctgcctct tttttcagga gtaaaacctt tgcttatgtt
    15661 ccttagcatc agcgagcatt atgcttctat tagccgagga gtactcgata gtagggattt
    15721 ggtgtacttc atctcgatga tttttctctt tatcattttt acccgtaccg ttttggaatc
    15781 gagaaaatgg taggggaatg caaaatggat tgctaaaatg gagttgaaga aaagttataa
    15841 ggtaaaaagt ttgctgcggt tactggtatc ggttgtcgct gttgcagctc tttatgcttt
    15901 atcaagcgtt ttgcatttcc gcatcgattt aacctccgac aagctttaca cccttcagcc
    15961 tatcaccagg gaaattctca aaaatttgaa tggtgaggtt tccatgaaaa tttatctcga
    16021 tggggattta ccaaccggct ttatccggat gaagaggatg ttgaccgaaa cgttggatga
    16081 gttcaatgtt gcctccaggg gtaaggttcg ctacgagctg gttaaccctt acagtgcaaa
    16141 aaactccagg gagcaggata gaattttcgc cggtctgtac gataaagggc tggcaccaac
    16201 cagcgttcag caacgaaaca ggcagggggg aagcagccaa acgattgtat tccccggatt
    16261 gcttatgagc tacaggggta aggagatggc cgttaacctg ttgaagaaca accctatgct
    16321 ttcggctgag gagaatttaa acctttctat tcagaatttt gaatttgctc tggttgatgc
    16381 cctcgtgaag ctttcaactg atagccttcc gcgaatagca tttattcagg ggcacggaga
    16441 gctagatgag tttgaaacag gcgatattga aaaatcgcta tcggagtact ttagggttga
    16501 tagggtgatt ctaaatggcg atatggaagc gcttaagccc tacagcgtag ctattgttgc
    16561 cggacccacg aagccttttc ccgaagaaga taagcttgtg gttgaccagt tcatcatgcg
    16621 gggtggaaaa gtgctatggt ttgttgaccc ggttgctgtt agcctggaca gcctttcaac
    16681 aggtgcatca accctggcct ttgttgccca aaccaacctc gatgatatgc tgtttcgtta
    16741 tggcgtgagg ttaaacccct tcatcgttca ggatttgcag tgtgctgtaa ttcctgtgaa
    16801 cattgctcca tccggtcagg cttccaagtt tgcccctgcg ccatggccct actttccgct
    16861 actaattcca ccgtcaaacc acccggttac gcggaatctg aaccccatac tcacccgatt
    16921 tgtaagtccc gtagatacgg ttggagagaa tcctttggta aaaaaacact acctgctcta
    16981 cacctctgct acaagccgaa ttctccaggt tcccctttta atcaaccttt ccgagattga
    17041 gcagacacca aacgagcagc attttataaa ccctaacgtt cccgtagccg ccgagcttga
    17101 aggatacttt acctcggttt ttaccaaccg tccgttgaag aattacaacc atggtgaacc
    17161 ttttgagttt cgtgctaaaa gctcgtttac ccgaatggtg gtagttgccg atgccgacat
    17221 ggttagaaac gatgtgcagc accgccctaa tggagcctat gtgcttccgt tgggatacga
    17281 ccgttacacc cagcaaacat acggaaacaa ggagctggtg atgaacatgg taaagtacct
    17341 gaatgatgat aatgggatag ttaaccttcg ctcacgcgac ttcaagctca ggctgcttga
    17401 caggagcaag gcttcccaat cgcgcttggc atggcaagtt gtcaacaccg ttttgccatc
    17461 gctagtgctg gtgctaggtg gcctaatcta tttttttatc cgaagaaatc attattctac
    17521 cccaaagtaa tgccaaccct ataacgtagt tagggcgcgg ctaatatccg tccctacttc
    17581 taaaatgtgg taaaccatta gaataagcca catttcaaga aaaaatatta aataggtagg
    17641 ctttttaaac tttaaacgga caaaaattcg taatattgaa cctgaaaatt taagtagatc
    17701 attctggtaa tcagtaattt ttaaattttg gcgcctaact tctttaagta tggcaagggt
    17761 gtttttactc tcaaaagata agtaaaagta gcattgataa taacttaaaa cttacgaaga
    17821 atgaaatttg tagtatcaag cactgagcta ttaagtcacc tttcggtggt aagcagggtg
    17881 attagcagca agaatacgct accgattctg gacaactttc tgttcaacct tgaggggaac
    17941 gagttggaaa ttactgcgtc cgatttggaa accacgctgg taacccgctt ttccctcgaa
    18001 aatgtttccg atgagggcac tgtggcaatt ccctacaaaa ttctgataga tacgctaaaa
    18061 gagttttccg aacaaccgtt gacttttgat ataaatcccg gtacccaaaa catcaccatt
    18121 aactctgaaa acggtcaatt tcacattgtt ggacagcctg ccgatgattt tcctcaaagg
    18181 gcaatgctga aacctgaaga ttcgcatagc gttaagcttt ctgccgaaat tctactcaat
    18241 ggcattaaca aaaccatatt tgccactgcc gatgacgaaa tgcggcctat gatgaacggc
    18301 atatttaccg agctcaacga acagggaata accttcgtat catccgatgc ccatcgcttg
    18361 gttcgctacc gcagaaagga cgttcaggtt gaagcggatg cctcgttcat tctacccaag
    18421 aaacctgccc agctactcaa gaatatgctg gtaaaggaaa ccggttttgt taacgtgcag
    18481 ttcgatgata aaaatgcggt gtttaccatg cctggctaca ccatggtttg ccgcctggtc
    18541 gaaggtaact atcccaacta taatgccgtt atccccgttg acaaccctaa tagaatgata
    18601 gttgacaggg ttgacctcta caactcggtt cgccgggtct ccatctactc caacgctgcc
    18661 agcaacatgg ttaaacttag cctcaccgga aaccagtgca ccgtttcggc cgaagacctc
    18721 gatttttctg tttcggcatt cgaaaggcta agctgtgagt acgagggaac cgaaatggaa
    18781 attggattta agtcaatttt cctatccgaa atattggcca acatttcctc taccgaggtg
    18841 gttctttctt tctcagaacc atctcttgcc tcacttgttt ttccccacat caacgaaaac
    18901 cccgacgagg atatattgat gctgcttatg ccaatgatga ttggcgatta agctagagct
    18961 taccaggcac cccggagctt ttaattccgg ggaatttttt cgggttgcat ggtaggcttg
    19021 tcgcatattc gacccactta agggtttgat ttacctttaa tgcaaggtta accatttata
    19081 tttgattaag attagcgttt attgcaccat ttcaacagct attgccatga agcttaatat
    19141 aaagaatccg ctcgccgtta ttgatttgga aacaacagga attgatatag tgcacgacag
    19201 ggtggttgag attgcagttc ttaaaatttt acccgatggc tcggagaata tgagggttcg
    19261 tcgggtgaac cccggaatgc ctattccgcc cgaggctact gccattcatg gcattaccga
    19321 tgaagatgta aggaatgaac caacatttaa ggaaatagct cgctcgctgg ctaaccatct
    19381 ggagggttgc gattttgcag gattcaactc taacaagttc gatcttcccc tgctggccga
    19441 ggagttcctg cgtgctggtg ttgatatcga cctcaagcgt cgaaagttta ttgatgtgca
    19501 aaccattttc cacaagatgg aaaagcgcac cctgggcgca gcctataagt tttacatgga
    19561 taaggagtta actgatgccc ataccgccgg agccgacacc atggctacct acgaaatact
    19621 catggctcag cttgaccgtt accccgagct taaaaacgac gtgaactacc tgtccgagtt
    19681 cagctcgttt aaccgcaatg ttgatttcgc cggccgaatc attttggatg acagaggagt
    19741 tgaagtattc aactttggta agcacaaagg gttgcctgtt gttgaggtgt taaaaaagga
    19801 gccaagctac tactcgtgga tgatgaacgg cgatttcccc ctttacacta aaagggtact
    19861 tacggccatt tacctgaaga tgaagcagca gtgatagtgt aaagggctaa tatcccttag
    19921 ggttcatggc gtgaggcgaa tggatgttta atacgttctt tagttgcagg aactattacc
    19981 cttagcccac gaggtactac gcttattttc aacttcttgc ccattattat tggctcacca
    20041 tcgtaatgca ccacatcctt atgcttacgc ttaattacca cctctgaaat ggcgccgctg
    20101 tgctccatag tacttttatc tatccgcttt gtaaagagcc ttatgccgat aaccggagcc
    20161 tcaaaaatct tgaaagggga gatgatgctt aggtttagct tcccgtctct tatgtctgcc
    20221 aagggtgcaa tgtaggcatt atttccgtac tgtgatgagt tggcaacagt gataaggaat
    20281 gccctaatct taaacttccc atcaccatca atctttagct tatacttttt cgatttgtag
    20341 cgtagaaaat cggagataac aatttttatg tagttgaaga atccgcgttt gccattactg
    20401 gcgaacctgt agcctatttg agcatcaaac ccaaccccgc aggtgcagaa gaatggaatt
    20461 ttattcacca tcccgtagtc aatcctatca gctgctccgc agttgatgat agccattgcc
    20521 cttttcaagt tcaacgggat tctcaaatga cgggcaagcc cgtttccaga acccgttgga
    20581 attatgccca gtgtagcctc ggtgccaaca attgcactgg ctacctcgtt cactgttccg
    20641 tcgcccccaa cagcaacgaa tttcgtatat ccttcggcaa gtttagcttg aacagccgcc
    20701 gttgcgtctc ctttgcatgt ggtaacatga aactcagccc ggtattttac attgtcgaga
    20761 tacttgagta tattcttttc cacgttgccc ttgggcctta tgccggaaat cggattgatg
    20821 ataaataaaa ttttttcctt ttctacctcc gttaaacgct ccatgggttt gttgtttgct
    20881 gggttgttgc ggttggtgca aatgtaagtt caaaagagga atagtgcaac agatgatgat
    20941 tttgcatggt ttcattggct tcatttttat tgctgctctt gcctcacaca aataatttta
    21001 ctttttttgt cggctaatta gttttcctat gagcacaacc gatagcagca ggcgagtaga
    21061 gctgatttct aacaggatgg gtataaagcc caggcaggtt tttaacgtta tccacctgct
    21121 ggaggagggc gcaaccattc cgtttattag ccgctaccgt aaggaagcga caggaaacct
    21181 cgacgaagtt caaattgccg aaattaactc cctctctgct aagcttctcg atctcgacag
    21241 gcgtaaggaa acaatcctga ccaccattga ggagcaagga aaattgaccg acagcctcag
    21301 ggagcgtatt gctggatgtt acgagttgtc tgagcttgag gacatctatc tgccctataa
    21361 accaaaacgc agaacccgtg caactattgc cagggagaag ggtctggaac cgcttgcctt
    21421 gctggtgttt aatcaggttg aaaaggatat tttaacaaaa gcccaaacct ttatatccga
    21481 taaggttgcc aatgttgagg aggcgttaca gggtgcacgc gatatagctg ccgaaatgct
    21541 aagcgaggat gaaagagtta gaaatactgt acgccgcaat tttaagcaga aagccattat
    21601 tcgttcaaga gttgtcaagg ggaaggaagc cgaaggcata aaatattccg attactttga
    21661 gtttgaagag cctgtccgac gctgcgcttc tcacaggttt ttggctatta aacgaggcga
    21721 agatgagggt tttctccggg tttccataac ccctcctgcc gatggcgttc ttgatgagct
    21781 acggaaatcg ctgctgaagt caaccggtga aacagccaag cagctcgaac ttgcactagc
    21841 cgattcctat aaaaggttga tagaaccatc aattgaaaat gagtttactg ccgagctaaa
    21901 ggaaaaggcg gatgctgagg caatacgagt atttgccgaa aaccttcgcc agctactgct
    21961 gtcccctccg ttgggacaga aaaatgtgct ggctattgat ccgggctacc gtacgggctg
    22021 caagatggtt tgccttgatg ctcagggaaa ccttatccat aacgaaacca tttatccaca
    22081 tcaaccccgc aacgagcagg gaaaggctat gtcaaaaatt tcaagccttg ttgaaacatt
    22141 caaaattgat gcaatagcca ttggtaacgg aaccgccggt cgcgaaacag agtcgcttgt
    22201 aaagcgtatt catttcaacc gagatgttaa ggtctttgtg gtcagcgagg atggcgcatc
    22261 tgtttactcc gcttctccag ttgcccgtga ggaatttccc gactatgatg taactgttcg
    22321 gggtgcagtt tccattggtc gtaggctgat ggacccgctt gccgagctgg ttaaaatcga
    22381 tccaaaatca attggggtag ggcagtacca gcacgatgtt gaccagtcaa agcttaaaga
    22441 ggggcttgac agggttgttg agagttgtgt taaccatgta ggggttaacc tgaatactgc
    22501 cagcatgcat ttgctaacct atgtctctgg cttaggacca cagctggcca aaaacattgt
    22561 tgattacagg aagcagaatg gcccattcag aagccgaacg gagctgaaga atgttccccg
    22621 gcttggtgga aaggcatttg aacaatgcgc aggctttttg agaattaccg aagcccaaaa
    22681 cccacttgac aacagcgccg ttcatcccga gagctaccat attgttgagc aaatggccgg
    22741 tgatttggag tgcacggtga aagacttaat tggcaacgac cagctaagat ctcgaattag
    22801 ggttgagaag tatgtttcag agaaaacggg gcttcctacc ctgaacgaca taatgctgga
    22861 actcgcaaaa cccggtcgcg accctcgtag cactattaaa gtatttgagt ttgccaagga
    22921 catttttaag attgacgacc taacccccgg catggtggtt ccgggaattg ttaccaacat
    22981 taccaatttt ggtgcttttg ttgatatagg tgttaagcag gatggactta tccacatctc
    23041 acagctggcc ggtaggtttg tttcaaaccc cctcgacgtg gtgaagctgc atcagcatgt
    23101 gcgggtaagg gtgcttgagg ttgatattcc ccgaaaaaga attcagctct cgctgaagga
    23161 tgttgagcaa tagtctatta gggaggcgga caga
//
LOCUS       DAOSHM010000037        22400 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_1858_length_22400_cov_20.687402, whole genome shotgun
            sequence.
ACCESSION   DAOSHM010000037 DAOSHM010000000
VERSION     DAOSHM010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 22400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22400
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1858_length_22400_cov_20.687402"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1105
                     /locus_tag="PK839_07065"
     CDS             <1..>1105
                     /locus_tag="PK839_07065"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOG72530.1"
                     /translation="GLTLEVGRKYDITGVLGWFNTSSIYQLYPRSAADVYAYSNDATL
                     STFTLGGQDVLALSGIVVANPATDPGATLHVDNFTGFAGIVAAATSAKATFAITLNGA
                     TVDPASYATQALADGDVVVATVTAEDGTVKHYKVTLSGENRTLTVTAPAGGESYSTGQ
                     PVTITWTSTNITSVNISVYDAISHTFLEQLNTTPVDATLGTFTYTIVNGEFGSIILRI
                     SDAADPAFYDESAGSFTVTDIAAPAITARFPVNNATDVAQNFTLSITFDEDVDAGTGN
                     LEIKKTADNSVVLTAAASEVTINNSTVTLSVSGLDYSTSYYVTLPAGFVVDQCGTNGN
                     AAIAANEWTFTTMEEQVSDLFFSEYVEGSSNNKA"
     assembly_gap    1106..1205
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1206..2721
                     /locus_tag="PK839_07070"
     CDS             <1206..2721
                     /locus_tag="PK839_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008823523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5018 domain-containing protein"
                     /protein_id="HOG72531.1"
                     /translation="ATAEHTLIRKASVTHGNTDWAASAGTTAENSEWEVHPQDYIANL
                     GTHSTTLSSAAEILTFTLPEQTGAATINSTNGTVSIEVLYGTNATALTPTITVSAGAT
                     INPASGVAQNFTSPVTYTVTAQDGTTTKVWTVTVTVASTPSNKADILSFNIPSQISSV
                     IDAQGATVSVVMPAGTNLTSLTPTITVSAGATINPASGVAQDFTNPVTYTVTAQDAST
                     KAWTVTVTVQQVDVVTIHDIQFSEATPADSPYKGQQVTTKGLVTGMKFSATYNNYSFF
                     MQDGVGAWNGVYVYACTFAVAVGDSVQLTATVDEYNTLTELKNVTALTVINSGNQAPA
                     PAEVSVTTAASEPYEGVLVKVTNAECTAAPDSYGAWTVSDGTTPILVDDLYFAYTPTV
                     GEHYNVTGIVDYAYSAWRMYPRETADVSLWNYTPANPFATFSAYPNPFTDVIRFSGSE
                     VNRVTIFSVIGQVVLDQQVNGAELVDTRNLEKGIYLVRFTNNQGKSTTRKLVKQ"
     gene            3076..3213
                     /locus_tag="PK839_07075"
     CDS             3076..3213
                     /locus_tag="PK839_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72532.1"
                     /translation="MRRRKLIKSGMLVALLAMVLVAGSCSKKTCPAYSDTNKAQVINR
                     A"
     gene            complement(3366..4706)
                     /gene="trkA"
                     /locus_tag="PK839_07080"
     CDS             complement(3366..4706)
                     /gene="trkA"
                     /locus_tag="PK839_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018694987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HOG72533.1"
                     /translation="MNIIIVGAGEVGTHLATMLAKEYHDVTVIDPDPEMLSHVTAVSD
                     VITLEGSAASISILKEANVKKADLFIAVAHSEETNIVSATLAKRLGAKKVIARIDRYE
                     YLQPNNKEIFLNMGIDSLIYPERLAAREVITLLGQTSSTEFVDFSGGKLSLLVFRLEE
                     DSPVLDKTLFQATRSIQNLEYRAVAIAREGETIIPRGNDQFKLNDLVYVIANQAGIKD
                     MLEYTGKENIDINNLMILGGSRIAIHLAGELENSMNVKIIEVDREKSQQLAEQFKNAL
                     VINGDGRDTDLLMEEGMQYMDAFVAVTGNSETNILSCAIAKSLGVKKTIAEIENMSYI
                     RLAESIGVDTVINKKLITASRIFRYTMSTDVSAIKCLTGSDAEVLEFIVKPGSPVTKG
                     KLCEISFPKDAIIGGVVRGSTAFIAKGDSEIKPYDRVVVFALPSAINRVGRFFN"
     gene            complement(4795..6903)
                     /locus_tag="PK839_07085"
     CDS             complement(4795..6903)
                     /locus_tag="PK839_07085"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HOG72534.1"
                     /translation="MSLPSSYHEVPFFRLLVPLVVGILVQLSLQPLHASMNILLLPVI
                     GFCILVVLSLLRKWELRWVYGLMLNLFMLVCGLVLSVNFTFNDDLSDLHEQGVVVRLT
                     ESPQLRARSLRAVGKVVRVMDKGSISPNEEKVLLYFSLTDSAAAQLQCGDIMAALLRF
                     STIEEPQNPYQFNFKQYMLARQIRFSSFVEPDSWARVGHHENGLVQLAGDLRRKLLAM
                     FSEFGLKDREYAVVSALTVGYTDLLDDALQKTYSTTGAMHILSVSGLHVGLVYGLLAF
                     LLSFLPKRAFFRIIKLPIQVVFLWFFALITGLSPPVCRSAFMFSLVAVGQGFNFRTNI
                     LNTLAAAAFALLVANPNNLLQVGFQLSFLAVLSIVVFHPFIYRLFTFKSKILDYFWSL
                     IAVSVAAQLGTIPVTLAAFGQFPTYFMLSNIIAIPLSTLILYAAVALVVFSPVPLVGA
                     FIGKILLVLTRSLNGGLQWIESLPHSSISGVYISRLQMLVLLSALVFISLYIVLKHSS
                     QLRAALLLMLFFFMLNIPQLRPVIRNELVVFHLPGSSMLCIRNNGLPIFIGTDSVGRN
                     LVEDSDFFLGGYISRQPSGRYGYINLCSPDWELLRQNGISLQRKYGLAVLEYNGRTVA
                     LPYSDSLRYATFGQTLNADVLVVNRFARGNLPDFIKPEVTVVDASVRFKHASAMLTRF
                     TGRGSAIHNLSVDGAYTAAL"
     gene            7271..7648
                     /locus_tag="PK839_07090"
     CDS             7271..7648
                     /locus_tag="PK839_07090"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HOG72535.1"
                     /translation="MDKLIIESRIDTPKVVADGNTGLFEITGKSLPEDAFEFYKPLER
                     YVQGYIKQPQPKTTINLKLEYLNSSSSKKLLDVIGYFEQLSKHGYEIELNWYHHDEDQ
                     DMIDEGTEFANMTSLRVNFIAEH"
     gene            7821..9707
                     /locus_tag="PK839_07095"
     CDS             7821..9707
                     /locus_tag="PK839_07095"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG72536.1"
                     /translation="MKKVVTILLILGLSVTPGFLPGQTIPAALQTEMEKNIELARSYE
                     TAGDINSALPLYTKTATTYWVNGNSEEARKLFIKAIALSERIGNTNALKMLLTNLGML
                     YVDEENYPEAIVNFEKCLSLNRKQNNKTEIASTLLNIANAQKELGQLQKSLNAAEEAS
                     KIAAELNDPKLMRNSYSLLSELYKEAGNDEKSAEYFTLYSAFSKKIQREEQLKKENEA
                     KKIVSEAQSQLHEIKTEKELTEQELMAKQHALTTVKDSLQKVEQLTREQRMEIDLLAK
                     ENALRDARIKNQILIRNIFIIAIILTLGIAGLITYYYLQKKRSNELLAHQKDMLEVKS
                     NELMEAIKQVEKQNRDITSSINYAQRIQQALLPTYNTLVTVINDSFIMLRPREIVSGD
                     FYWFAGYTGADSSTSTSNRHFIHVPSLKEDEGGFLIAAVDCTGHGVPGAFMSMIGLNL
                     IDNLVRTGRVRPDLLLGDLHKLIRYLLKQEESDNRDGMDIAICNIKNDGQTVEFAGAK
                     NPLFYVTNGQVNQVRGDPVPVGGLQKEKERQYTLHTIHIDTPTCFYIFSDGYIDQFGG
                     PYNQKFSSQQFKELLLKVHQLPMIQQQEILETTMDEWQGSENKQIDDIMVIGFRLNAK
                     PLGI"
     gene            9848..10804
                     /locus_tag="PK839_07100"
     CDS             9848..10804
                     /locus_tag="PK839_07100"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOG72537.1"
                     /translation="MRSWLLTLVLLPYLFGTLAGQERQENLYVNEANQIFVKADAPIY
                     FFVSPSGQPQNMIMLPSSDSLANPMYFDGDGKHYLVYQSSGQKVRYLVYADGVGPNPY
                     VEVARGLLMKHQNRIYLEPNATFTPKAKDKRSGVKSVYYSLNGEPYKLATNELSPGSN
                     GEFSLHIYAVDNVGNTSDTAAYTLISSPDVTFRVNNIYFETASVNLLPESFEVLNQME
                     DMLKLYPELHVEIKAHADTRGNTDYNLDLSERRAQSVVRYLSGKGISESRLKAVGFGD
                     SQPVNECLKGVQCPDSKHRENRRIEFRFFIPKLMQPTPKLSL"
     gene            10806..12494
                     /locus_tag="PK839_07105"
     CDS             10806..12494
                     /locus_tag="PK839_07105"
                     /inference="COORDINATES: protein motif:HMM:NF025125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOG72538.1"
                     /translation="MKGLLKYLFALTFAILLSHSIFAQNATRVLYMDTTGKMLYASPN
                     VEVNLYINTTNSASGAVKLTGLEGKGNPLRWSGHGLQVMTHLNLYIGRKIRFELFADG
                     KPPVSAVTSTNRNQKRIDEAVYIPGGAVIEVNAADENSGLEDTYYSLNGEPFVPYTSP
                     LALDKDGVYEFTVYSTDKLGNREDEVTRKFVVDATPPTSAITVEGDQFEQVVSGRSVF
                     KLSATDEFGVKEILYRIDSSKFEAYRYPIKAGSLTDGDHVLEWYATDMVGNSEALQSH
                     HFTVDKTPPIVVEEIAGNTYMVGNVEYSSGRSQLRIVAIDNRAGIKDIFYSLNNGEYI
                     RYEKQVMLSDILGTINVKTYAVDNVNNRSQSGANTESFVMPTIDITGPTIGYQLLGRQ
                     LTLRDTLWIGQSTLVKLNAADKESGANRILYTIDKSGGMDYTGPFSLTEPGFHEIRCT
                     AYDNVDNLNFLSFKLGLDSEAPKIYHHFSVEPFKELPVGNVPVYPASVKLYLAATDNL
                     TGGVSVKYSVNEGKLTDYTQPIENFKPGNNYTLSITATDELGNSSTQTINFRVE"
     gene            12938..14851
                     /locus_tag="PK839_07110"
     CDS             12938..14851
                     /locus_tag="PK839_07110"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HOG72539.1"
                     /translation="MNLHRYTIKKLLSFTSTIILLSFLLFTSIYYIRQQAVRQDKNIL
                     QRLDRLEQNSLRIKMLQDEFLLRDFHNSQLYVLGQSPATLRLDSLFTKTLEELNYLKK
                     YFSSTKITEVERHIEQYKKNFNNLKELEITRGFRDYGLEGDLRNIIHQVEGNAVLNND
                     YRLLSGMLMLRRHEKDYMLRRDTLYVEKFDATLASTLNYAADKYGANNQFSKQLIDYS
                     KLFHEFLSIDAQIGRTENEGATYQLQQQSALVIESIRNLYGELSENIKKKDREAYASM
                     LMLLVIVSSLVLTLLTKISRHITKTIKSTLAVIKKLGKGELPPPLEVKGADEFSAMEK
                     SINDLSLALRNTRDFAIEVGNGNFYSEVNVFGNSGELGSGLLEMRQKLTEVAVQQENS
                     LKEKEQRSWLNECLAQVSELLRNFHSDTKELCFEFVRFAIKSTGSLQGGIYLLNSEGE
                     SDSDENSYLELVASYAYDRRKYINKRFEKYEGVVGSCLYEKDVIYITDIPDSYTHITT
                     GLGVSNPSCIVLTPLVTEKEEALGVMEISSYSPITDIQVEFMKQACRNLASSLILHKA
                     IKANEQIIAQMKVKTEELLANEEELRQNMEELTAIREDMERREKDLNGQIAELKYKLR
                     LHGIIFEADTYHN"
     gene            complement(14918..15589)
                     /gene="ung"
                     /locus_tag="PK839_07115"
     CDS             complement(14918..15589)
                     /gene="ung"
                     /locus_tag="PK839_07115"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009292592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOG72540.1"
                     /translation="MNPQIEESWKKVLAGEFQQEYFRELKNFLVEERRQYRVYPPGSK
                     IFAAFDFTPFTSVKVVLLGQDPYHGQGQAQGLCFSVPRGIPKPPSLVNIFKELHDDLG
                     LAIPPHGNLEEWARQGVLLLNATLTVRANQAGSHQHKGWEQFTDAAIRALAEQRENLV
                     FMLWGNYAQAKAALVDGHKHCILKAPHPSPFSANQGFFGCRHFSRANDFLVRAGKEPV
                     DWQIS"
     gene            15920..16489
                     /locus_tag="PK839_07120"
     CDS             15920..16489
                     /locus_tag="PK839_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOG72541.1"
                     /translation="MNADQQTSEQFYWLVRNRQSDRAYSNRPVDREILVRVVEAARLA
                     PSACNAQPWKFIVVDDPELKNRLADAAASKILGINHFTKQAPVHIVIVLEGANLNSNF
                     GSMVKGKTLPLIDIGIAAEHICLAAKSEGLGSCILGWFDEKAVKQLLDIPKSKRVPLI
                     ITLGYPKTEDIREKRRKPLEKIISFNGYS"
     gene            complement(16494..17594)
                     /locus_tag="PK839_07125"
     CDS             complement(16494..17594)
                     /locus_tag="PK839_07125"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOG72542.1"
                     /translation="MKAIKVSFCTTSNLITDQRIQRAASTLSRNGYQVNVFALRRHGL
                     PDEKPFEFAVKRFRCVFSDGPMFYVEYNLKILTRLIFTRTSLIYSNDLDTLPGCWLAS
                     ILKAKPLVYDSHELFTEIPELIGHPVKKRLWAIAERVCIRRAKCVITVSEGVAKELST
                     RYGVKPVVVRNFSVKRDKSPYRDLKPTLIYQGSLNVGRGIELAIDMMEWLPCYHLLVI
                     GSGDIEIPLRRRAIERGLTDRVEFMGRVDPDRLPDVTGRAWLGLSLEEDLGLNYRLAL
                     PNKIFDYIASGVPILVSDLPEMRKIVETYHVGLVANTRDPEKLAGIVADFVENKKARE
                     VMINNVLAASETLCWDNEYPVLLNAVGKAIGC"
     gene            complement(17711..19945)
                     /locus_tag="PK839_07130"
     CDS             complement(17711..19945)
                     /locus_tag="PK839_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HOG72543.1"
                     /translation="MEIYYLIILILLFVLAISDLMVGVANDAVNFLNSAIGSRVAARH
                     WILLVASVGVLVGAVFSSGMMEIAQKGVFRPEKFYMSEIMTLFMAVMLTDVIVLDFFN
                     TFGLPTSTTVSLVFELLGSAVAISIFKISENNESIADLGSYINTAKALAMISGIFISV
                     VIAFFIGTIVQYIARVLFTFNYTRSMKYVGSIWGGISLTAITYFIAMKGLNSTALVSP
                     DTLNFINQHTMVILLLSFTGWTIIFQLAISIFKWNILRFVVLAGTFALALSFAGNDLV
                     NFIGPTMAALKTYQVHLANPGVAPDQLLMTDLAGPVSTNTLYLLIAGLVMVITLWTSR
                     KARTVTNTEVGLANQDSGAERFGSTQLSRSIVRISRSFSEGVGRITPLRIKSFLSRRF
                     DLSKALVVKNPKDAPAFDLVRASVNLVVSSILISLGTSLKLPLSTTYVTFMVAMGTSL
                     SDRAWGRESAVYRVTGVITVIAGWFFTALITFIVSFVVAAALFYWKAFALVGLAILAG
                     WLLIRSNLIHRSRNQNRDVGAELITDKSVVEKCTLGVNNSLRDVVEVFSQTIDGLTSE
                     NRKLLRTVNNRVADLNSNAKNFKNNIYQTLKKLEADSVETGHYYVQVVDYLREIAHSL
                     SFITEPSLKHIENQHKGLTTDQAIELTTISNQVSDFFQEIMKIIKNNDFTGVPQVVSK
                     QQDILDKLNEARKNQVKRIKQGVTGTRNSVLYLGLINEIKNIMLHTVNLLKAQRDFIL
                     NNVE"
     gene            complement(20052..20807)
                     /locus_tag="PK839_07135"
     CDS             complement(20052..20807)
                     /locus_tag="PK839_07135"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOG72544.1"
                     /translation="MFTALIVDDELNSRQTLAYLLSHFCPTVRVIGEAEGVESAYTSI
                     QKLNPDIVFLDLQLADGNGFDLLRMFEEVHFMLIIVTAYQEYAIRAFKYCALDYLLKP
                     IDVNSLVDAVDRLQHSFSSQEANRKFQVLLSNTSACSKKCKKLVLKSLEGLRVVETDE
                     IVRCESHNNTTEFILTTEQGVMVSKTLKEYEELLAPEGFIRCHQSHLINKRHVKEVLR
                     FPSPTIRMDNGDEVPVSVRKRDILRAIAGKQNG"
     gene            complement(20810..21541)
                     /locus_tag="PK839_07140"
     CDS             complement(20810..21541)
                     /locus_tag="PK839_07140"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOG72545.1"
                     /translation="MNGCLFFEIFTVAITMVAVVLVLAYRRLKRKYLRLEQQLLLSQL
                     NPHFVFNSLTAIQSYVFRNEAHLASKYLASFAKLTRLILENSRSELCSIENEVATLEH
                     YLELQSLRFSNRFDYSIYVDEKIDADSTLIPPMLTQPFIENAIEHGFNGIEDRGRLTI
                     SYLYLDKHHVEIEVKDNGVGIKRSEQANPASGKDHLSLATEITRERLKNIRHYKRLNI
                     ILTLVDTGSIDPKQHGTLVKFKISI"
     gene            complement(21713..21982)
                     /locus_tag="PK839_07145"
     CDS             complement(21713..21982)
                     /locus_tag="PK839_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HOG72546.1"
                     /translation="MKEGIHPQNYRLVAFKDMSNDHVFITKSAANTKDTIVIDGVEYP
                     MVKVEISNTSHPFYTGKMKLVDTAGRVDKFMNRYKKHMDNNKAKQ"
BASE COUNT         6514 a         5476 c         4992 g         5318 t
ORIGIN      
        1 ccgggctcac cctggaggtt ggccgcaagt acgacatcac gggagtgctt ggctggttca
       61 acacctccag tatctaccag ctctacccac gaagcgcagc cgatgtttac gcctacagca
      121 acgacgccac cctatcaacc ttcacgctgg gcggacagga tgtgcttgcc ctttcgggca
      181 tagtagttgc caaccctgcc accgacccgg gcgcaacact ccatgttgac aatttcaccg
      241 gttttgcagg aattgttgct gctgcaacca gcgccaaggc cacctttgcc ataaccctca
      301 acggagcaac cgttgacccg gccagctacg ccacccaggc ccttgccgat ggcgacgtgg
      361 tggtggccac cgttaccgca gaggatggca ccgttaagca ctacaaggtt accctgagcg
      421 gtgaaaaccg caccctgacg gttaccgcac ccgctggcgg cgaaagctac agcaccggtc
      481 agccggttac catcacctgg acatccacca acatcaccag cgtgaacatc agcgtgtacg
      541 acgccatatc gcacaccttc ctggaacagc tcaacacaac acccgttgat gctactctgg
      601 gaacgtttac ctacaccatc gtaaacggcg agtttggatc tattatcctc cggatttccg
      661 atgcagccga tccggccttc tacgatgaaa gcgcaggttc tttcacagtt accgacattg
      721 ccgccccggc cattaccgcc aggttccctg taaacaacgc caccgacgtt gcccagaact
      781 tcaccctctc catcaccttc gacgaggatg tggatgccgg aaccggcaat ctggaaatca
      841 agaaaacggc cgacaactcg gttgttctca ccgcagctgc ttcagaggtt accattaaca
      901 atagcacggt taccctgagc gtttcggggt tggactacag cacctcctac tacgtaaccc
      961 tacccgcagg ctttgtggtt gaccagtgcg gaactaatgg caacgccgcc attgccgcca
     1021 acgagtggac atttaccacc atggaggagc aagtaagcga cctgttcttt agcgagtatg
     1081 tcgaggggag cagcaacaac aaggcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnntgcca cagccgagca taccctaatc cggaaagctt cggttaccca tggtaatacc
     1261 gattgggctg cttcggccgg tactacagca gagaactccg aatgggaagt tcatccgcag
     1321 gactacatag ctaacctggg aacacactca acaaccctat catctgctgc cgagatactc
     1381 acctttacgc tacccgaaca aacgggtgct gcaaccatta actccaccaa cggaactgtc
     1441 agcatcgagg tgctgtacgg caccaatgca accgccctca ccccaaccat cacggtttct
     1501 gcaggggcta ccatcaaccc ggccagcggc gtggctcaga acttcaccag tcctgttacc
     1561 tacaccgtaa ccgctcagga tggcacaaca accaaggtgt ggacggttac cgttaccgtt
     1621 gcctctacac ccagcaacaa ggccgatata ctctccttca acatcccaag ccagatatcc
     1681 tcggtgattg atgcccaggg ggcaaccgtt tcggtggtta tgcctgccgg tactaatcta
     1741 accagcctca ccccaaccat cacggtttcg gcaggggcaa ccatcaaccc ggccagcggc
     1801 gtggctcagg acttcaccaa cccggttacc tacaccgtaa ccgcacagga tgccagcacc
     1861 aaggcatgga cggttaccgt taccgtgcag caggttgatg ttgttaccat tcacgacatc
     1921 cagttctccg aggctacccc tgccgattcg ccctacaagg gacagcaggt aaccaccaag
     1981 gggctggtta cggggatgaa gtttagcgcc acatacaaca actactcctt cttcatgcag
     2041 gacggcgttg gtgcatggaa cggggtttac gtttacgcct gcacatttgc agttgccgtt
     2101 ggcgatagcg ttcagcttac cgccacggtg gatgagtaca acaccctcac cgagctgaag
     2161 aacgttaccg cgctaacggt tatcaactca gggaatcagg caccagctcc ggccgaggtc
     2221 agcgttacca cagctgccag cgagccctac gagggcgtgc tggttaaggt aaccaatgct
     2281 gaatgcactg ctgcccccga ttcctatggt gcatggacgg ttagcgacgg aaccactcca
     2341 attctggttg acgacctata cttcgcatat acccctaccg taggcgagca ctacaacgta
     2401 acgggtattg ttgactacgc ttacagcgca tggaggatgt acccacgcga gactgccgat
     2461 gtaagccttt ggaactatac tccggccaat ccttttgcaa ccttcagcgc ttaccccaac
     2521 cccttcaccg acgtgataag gttcagcggt agcgaggtta accgggttac catcttctcg
     2581 gtaattggtc aggtggttct cgaccagcag gttaacggtg ccgagttggt tgacacgcgc
     2641 aacctggaaa agggaattta cctggtaagg tttaccaaca accaggggaa atcaaccacc
     2701 cgtaagctgg ttaagcagta accaaccaaa ataactacaa aacccccgta acttgggggt
     2761 tttgtttttt acataagtga acttctattt tattcgtcaa tattttttat tcactggtca
     2821 aggtatttta aacctaagtc aaatatattt tagtaacttg cgtaatattt ctttattgca
     2881 acaatattaa agcagcattg ttacttaggg taatacaggg gtatcctaaa aatgaaaaag
     2941 aaaataactc caattgttat tgggctgctg ttctgcctga tgatgctgaa ctcctgccac
     3001 aacaacatac cgtgccctgt atatgcagat gtggatattt gtgaaaaaac tggtaactga
     3061 ttaataacta ataatatgcg aagaagaaaa cttattaaat cggggatgtt ggttgccctg
     3121 ctcgccatgg tactcgtagc tggttcgtgc agcaagaaaa cgtgtcctgc ctatagcgat
     3181 acaaacaaag cacaggttat caacagggct taagtccgat ggttagcctg atttaggcat
     3241 cagataataa tatcgtttaa aaaatatcct ctcgaacgct ttaggggtta cctgaacacc
     3301 tgaagcgttt agttatacac ttaatgttag ttgggaaaac ttctacaagt ccaacccgaa
     3361 aactgctagt tgaagaaacg cccaaccctg ttaatagccg aaggaagggc aaaaaccacc
     3421 acccggtcgt agggtttaat ttcggaatcg cccttggcaa taaacgccgt gctaccccta
     3481 acaaccccgc caataatggc gtccttggga aaagaaattt cgcaaagctt ccctttagta
     3541 accggagaac ccggcttaac gatgaactcc agaacctcgg catcggaacc ggtaaggcat
     3601 ttgatggcgg acacgtcggt gctcatggtg tagcggaata tgcggctggc tgtaatcagc
     3661 tttttattga taaccgtgtc caccccaatg ctctcggcca accgtatata gctcatgttt
     3721 tcaatttcgg caatagtctt cttcaccccg aggctttttg caatggcgca cgaaaggatg
     3781 ttggtttcgg agttaccggt aaccgccaca aatgcatcca tgtactgcat accctcctcc
     3841 ataagcaggt cggtatcgcg tccatcaccg ttaatcacca gcgcattctt aaattgctcg
     3901 gccagctgct gactcttttc acggtccacc tctataatct taacattcat ggagttttcc
     3961 agctcaccag ccaggtgaat ggcaattcgg ctaccgccaa gaatcatcag gttgttgatg
     4021 tcaatattct cctttccggt gtattcaagc atatccttaa tgccagcctg gttggcaatg
     4081 acgtaaacca aatcgttcag cttaaactgg tcgttacccc ggggaataat ggtttcacct
     4141 tcgcgggcaa tggcaaccgc cctgtattct aaattttgaa tgcttcgggt ggcctggaat
     4201 agggttttat ccaaaaccgg ggaatcttct tcgaggcgga aaaccagcaa cgatagcttg
     4261 ccacccgaaa aatcgacaaa ttcggtggag ctggtttgac ccaacagggt aattacctca
     4321 cgggcggcta acctctccgg gtagattagg gaatcgatcc ccatgttaag gaatatctcc
     4381 ttattgttgg gctgaagata ctcgtagcgg tcaatgcggg ctatcacctt tttagccccc
     4441 aggcgcttag ccagcgtagc ggaaacaatg ttggtctcct ccgaatgggc aacggcaata
     4501 aacaaatcgg cttttttcac gttggcctct ttgaggatac tgatggaggc agccgaacct
     4561 tcgagggtta ttacatcgga aacagcggta acatggctga gcatttcagg atcgggatca
     4621 atcaccgtta catcgtgata ctccttggcc agcatggtag caagatgcgt tcctacctca
     4681 cctgccccaa caatgattat attcatgaca ccctaaaaag aggttttagt gcatatttta
     4741 cagctgcaaa gtaatactaa aaacagcaaa cccattgcca ggttttcaat aattttagag
     4801 ggcagctgta taggctccgt caaccgacag gttgtggatg gccgaccccc tgccggtaaa
     4861 cctggtaagc atcgccgaag catgtttaaa cctaaccgag gcatccacaa cggtaacttc
     4921 gggtttgatg aaatcgggaa ggtttccacg ggcaaagcgg ttaaccacca gaacatcggc
     4981 attgagcgtt tgcccaaatg tagcataccg gagcgaatcg ctgtagggta acgcaaccgt
     5041 tcgaccatta tactccagca cagccaatcc gtattttcgc tggagcgata tgccgttttg
     5101 tcgcagtagc tcccaatcgg gggagcacag gttaatgtag ccatagcgcc ccgagggttg
     5161 ccgggagata taccctccca ggaaaaaatc gctatcctca actaaattcc gaccaaccga
     5221 atcggtgcca ataaatatgg gcagcccgtt gttccgaata catagcatag agctgccagg
     5281 aagatggaaa accaccagct cgttccggat tacaggtcta agctggggaa tattcagcat
     5341 gaagaaaaaa agcataagta aaagtgctgc acgcagctgc gatgaatgct tcagcacgat
     5401 atacagcgag atgaacacca acgccgagag cagaacaagc atttgcagcc tggagatgta
     5461 aactcccgat atggaggaat gcggcaggct ttcaatccac tgcaatcccc cgttaagaga
     5521 acgagtaaga acaagcagaa ttttgccgat gaatgcccca accagcggaa caggcgaaaa
     5581 aaccaccagg gctacagccg catacagaat tagcgtggat aacggaatgg ctataatgtt
     5641 ggatagcatg aagtaggtgg ggaattgccc aaaagcagcc agggtaacgg gaattgttcc
     5701 tagctgggca gccactgaaa cggcaatcag cgaccagaag tagtccagaa ttttgctttt
     5761 gaaagtaaac agcctgtaga taaaggggtg aaaaaccacg atagagagca ccgccagaaa
     5821 cgaaagttga aaccccacct gcagcaggtt gtttggatta gctacaagaa gggcaaatgc
     5881 tgcagcggcc agcgtgttta gaatattggt gcgaaagtta aagccctggc caacggcaac
     5941 cagcgagaac atgaaagccg aacggcaaac aggcggggat agtccggtaa taagcgcaaa
     6001 aaaccagaga aataccacct gaatgggtag cttaattatc cggaaaaaag cccgtttcgg
     6061 gaggaagctg agcaaaaagg ccagcaggcc ataaaccagg ccaacatgca aaccggaaac
     6121 ggaaagaatg tgcatggccc cggtggttga ataggttttc tggagcgcat cgtcgagcag
     6181 gtcggtgtag ccaaccgtta acgctgaaac aacggcatac tctctatcct taagcccgaa
     6241 ttcggaaaac atagccaaca gctttctacg caagtctcct gccagctgaa caagaccatt
     6301 ttcatgatgc ccaaccctgg cccagctgtc aggctcaacg aagctgctga agcgaatctg
     6361 gcgggcaagc atgtactgct taaagttgaa ttggtagggg ttttgtggct cctctattgt
     6421 gctgaaccta agaagggcag ccataatatc cccgcactgc agctgagcgg cagcagaatc
     6481 ggttaacgag aagtaaagca aaaccttttc ctcgtttggt gaaatgctcc ccttatccat
     6541 cacccgaacc actttcccaa ctgcccgcag ggaacgggca cgtagctggg gactttccgt
     6601 caaccttact actacacctt gctcatgtaa atcggaaagg tcatcattga aggtaaagtt
     6661 aacggaaagc acaagcccgc agaccagcat gaaaaggtta agcattaggc cataaaccca
     6721 gcgcagctcc cattttctca gcagtgatag caccaccaga atgcagaacc ctatcaccgg
     6781 taaaagaaga atgttcatgc tggcatgaag cggttgcagg ctcagctgaa cgagaatccc
     6841 cacaaccagc ggtacaagca gcctgaaaaa tggcacctcg tggtagctgg aaggtaacga
     6901 cactgcagta gcaattaaac ttataaacaa gttaatagaa ttccactgct tgtcaaaaaa
     6961 aaaaaattaa tccaacaggc tgttaactcc tcaagagcaa aaaatgattt aacggaaaaa
     7021 tgcagctact accattttaa tcccttgaga aataccccgc gaatggaaaa aggcaacact
     7081 aggcttttac caacataagg cagctgcgcg gtataaattt taaataaaaa ccgtagggca
     7141 gctgtagatt aaacggcctg ttattaatgc agttaagaaa taagaacggc cttagaatac
     7201 agattatgcc ctgcttatgg taaaaccttt ggttacaatt ttgcgtaaat attgaaataa
     7261 aatgagcaac atggataaac taataattga aagccggata gatactccaa aagttgtggc
     7321 tgatggcaat accggcttgt ttgaaattac cggcaagtcg ctacccgaag atgcctttga
     7381 gttctacaag cctctggaaa ggtatgttca gggatacatc aaacagcccc aaccaaaaac
     7441 cacaataaac ctaaagctgg agtacctcaa ctcctcatcc tccaaaaagc tgcttgatgt
     7501 gattggttac tttgagcagc tatcaaagca tggttacgaa attgagctta actggtacca
     7561 tcacgacgag gatcaggata tgatagacga gggtacagag tttgccaaca tgacctccct
     7621 tcgggtaaat tttattgccg agcactaggc atacccatgc ggttgtgcag caagcactta
     7681 gtagtaatat cacactaatc cagattttgt cctgtaaccg gtgtataagg cgctaacatg
     7741 gctaacgcac aagaaggcaa gtaggagttc gagtttttaa aagcaaatta tttaggagca
     7801 cacataatac cgaatatgtt gtgaaaaaag ttgtcaccat acttttaatt cttggactta
     7861 gcgttacgcc tggttttctc cctggacaaa ccattcctgc agcccttcag accgaaatgg
     7921 agaaaaatat tgagttggcc agaagctacg aaactgcagg cgacattaac tccgccctac
     7981 ccctttacac caaaacagcc accacctact gggtaaacgg gaattccgag gaggcaagaa
     8041 aattgtttat caaagcaatt gcgcttagcg agcgtattgg aaataccaat gccctcaaaa
     8101 tgcttttgac caaccttggc atgttgtatg ttgacgagga aaactacccc gaggccattg
     8161 tcaactttga gaaatgccta tcgctcaacc ggaagcaaaa caacaaaacg gaaatagcct
     8221 caaccctgct aaacatagcc aatgcccaaa aggagttggg gcagcttcaa aaatcactca
     8281 acgcggcaga ggaagccagc aaaattgcag ccgagctgaa cgaccccaag ctgatgcgaa
     8341 actcatactc gttgctttcg gaactctata aggaagccgg caacgacgaa aaatcggctg
     8401 aatactttac gctctactcc gcattttcaa aaaaaattca acgcgaggag cagcttaaaa
     8461 aggaaaatga ggctaaaaaa atagttagcg aagcccaaag ccagcttcac gaaataaaaa
     8521 cagaaaagga gcttaccgag caagagctga tggcaaaaca gcatgcgcta acaaccgtta
     8581 aggacagcct gcaaaaggta gagcaactca cccgcgagca gcgtatggag attgacctac
     8641 ttgccaagga aaatgcctta agggatgcca gaataaaaaa ccaaattctt atccgtaaca
     8701 tattcatcat tgccataata ctcaccctgg gcattgcagg gcttattacc tactactacc
     8761 tgcaaaagaa acgctccaac gagctcctgg cacaccaaaa ggacatgctg gaggtaaaaa
     8821 gtaatgagct aatggaagcc attaagcagg ttgaaaagca aaaccgcgac atcacgagca
     8881 gcattaacta tgcccagcga attcagcaag ccctgcttcc aacctacaat acgctggtta
     8941 ccgtgatcaa cgactcgttc ataatgcttc gcccacgtga aattgtaagc ggcgactttt
     9001 actggtttgc cgggtacacc ggcgccgatt cgtcaacgtc aacatcaaac cggcatttta
     9061 tacatgtgcc cagtcttaag gaggatgagg gtggatttct aatagcagcg gttgactgca
     9121 caggtcatgg ggtgcctggt gctttcatgt ccatgattgg gctcaacctg attgacaacc
     9181 ttgtgcgaac gggaagggtt cgccccgacc tacttctggg cgaccttcac aagctaatca
     9241 ggtatctcct gaaacaggag gagagcgata acagggacgg catggatata gccatttgca
     9301 atatcaaaaa tgatggacaa acagttgaat ttgccggcgc taaaaaccct ctattctatg
     9361 taaccaacgg tcaggttaac caggttagag gcgatcctgt gcctgttggt ggactacaaa
     9421 aagaaaagga acgccaatat accctccaca ccatccatat tgacacccca acctgcttct
     9481 acattttctc cgacggctac atcgaccagt ttggaggccc gtataaccag aaattctcat
     9541 cacagcaatt caaggagctg ttgcttaagg ttcaccagct tcccatgatc cagcaacagg
     9601 aaattcttga aactactatg gatgaatggc agggaagtga aaacaagcag atcgacgata
     9661 taatggtcat tggttttagg cttaacgcca aacctttagg catatagcac ccaaaagcaa
     9721 tgctgtaggc catttcacaa acccgatgcc ttcatctgct cccttgttga tctaaaaaaa
     9781 agtaattttt acgattaggg aattgagcaa gaaaatatta taaatttgtc ccgacaaaga
     9841 gcgcaatatg agaagttggt tgcttaccct tgttctgctg ccatatttgt ttggaactct
     9901 agccggccag gagcggcagg aaaatcttta cgtcaacgaa gcgaatcaaa tattcgttaa
     9961 agccgatgcc cccatttact tttttgtttc tccttctggt caaccccaaa acatgattat
    10021 gctgccaagc agcgatagcc tggctaaccc aatgtacttc gatggtgacg gaaagcacta
    10081 cctggtttac cagagtagcg gccaaaaagt tcgctacctg gtgtatgctg atggagttgg
    10141 tcccaacccc tacgtcgagg ttgcccgggg cctgcttatg aagcaccaga accgcatata
    10201 ccttgaaccc aacgcaacct tcactcctaa ggcaaaggat aaacgttcgg gggtaaaatc
    10261 tgtttactac tccttaaacg gcgaacccta caagctggcc accaacgagt tgtcgccggg
    10321 tagcaacgga gaattcagcc ttcacattta tgctgttgat aatgttggta atacaagcga
    10381 taccgcagca tacaccctaa tttcttctcc cgatgttaca ttcagggtaa acaacattta
    10441 ctttgaaact gccagcgtaa acctgctacc cgaatcattc gaggttttaa atcaaatgga
    10501 agacatgctg aagctctacc ccgaactcca tgttgaaata aaagcacatg ccgacacgcg
    10561 aggaaacact gattataacc ttgacttgtc cgaacgaagg gcacaatcgg ttgttcgtta
    10621 cctttcgggc aagggcatca gcgagagcag gctgaaggcc gttgggtttg gcgattcgca
    10681 accggtaaac gaatgcctga aaggggtgca gtgtcctgat agcaagcacc gggagaaccg
    10741 gagaattgaa tttcgcttct ttattccaaa actcatgcag cccacaccca aactcagctt
    10801 ataaaatgaa aggactgcta aaatacctat tcgccctaac ctttgccatt cttttatcgc
    10861 atagcatttt tgcccagaat gccacacgtg tcctgtacat ggatacaacc ggaaaaatgc
    10921 tctacgcatc acccaacgtg gaggtaaatc tctacatcaa cacaaccaac tcagcctcag
    10981 gtgcagtaaa actcacaggt ctggaaggaa aagggaatcc tttgcgctgg agcggacacg
    11041 gacttcaggt gatgactcac cttaacctct acattggccg aaaaattcgt tttgagctgt
    11101 ttgccgatgg taaaccgcct gtatcagcgg taacctccac caaccggaac cagaaacgta
    11161 ttgacgaggc tgtgtacata cctggcggag cggtaattga ggtgaatgcc gccgatgaga
    11221 actcaggcct tgaggatacc tactactctc tcaacggcga accctttgtt ccctacacct
    11281 ctcccctggc actcgacaag gatggagttt acgagtttac cgtgtactca accgacaagc
    11341 tgggcaaccg ggaggatgag gtaacccgta aattcgtggt tgatgctaca cctcctacat
    11401 cggctattac cgttgaaggt gatcagtttg aacaagtagt atcgggacgg tcggttttta
    11461 agctgagcgc caccgatgag ttcggtgtga aagaaatcct ctaccggatt gactcgtcaa
    11521 aatttgaagc ctaccggtat ccgatcaagg caggaagtct gactgacgga gaccacgttt
    11581 tggaatggta cgccaccgac atggtgggta atagcgaagc cctgcagagc caccatttca
    11641 cagtggacaa aacaccacca attgtagtgg aggaaattgc cggcaacacc tacatggtgg
    11701 gcaatgtgga gtactcatcg ggtcgaagcc agcttagaat tgttgccatt gacaacagag
    11761 ccggcattaa ggatattttt tactccctta acaatggcga gtacattcgc tatgaaaaac
    11821 aggtaatgct ctcggatata cttggaacca taaatgtgaa aacttacgcg gttgacaatg
    11881 taaacaaccg cagccagtca ggagcaaaca ccgaatcgtt cgtaatgccc accattgaca
    11941 taaccggccc aaccattggt taccagctgc tgggccgcca gctaaccctc cgcgacaccc
    12001 tttggattgg acaatcaacg ctggtaaagc taaatgctgc cgacaaggag tcaggagcaa
    12061 accgcatctt atacaccatt gacaaatcgg gtggcatgga ctacaccggc ccatttagcc
    12121 tgaccgaacc cggattccac gaaattcgat gcaccgccta tgacaacgtg gataatctta
    12181 acttcctatc gttcaaacta gggcttgata gcgaagctcc caaaatctac catcacttta
    12241 gcgttgaacc ctttaaagag ctacctgttg gtaatgttcc cgtttacccc gcaagcgtaa
    12301 agctttacct ggctgcaacc gataacctga cgggaggtgt ttccgtaaag tattcggtta
    12361 atgagggcaa actaaccgac tacacccaac cgattgagaa cttcaaaccc gggaataact
    12421 ataccttatc catcacagcc accgatgagc tgggaaacag ctcaacacaa accatcaact
    12481 ttagggtgga gtaggttcca accggagaga cttgcccgaa aacacagcat gaatagcaac
    12541 accggggaga atttcatttt cctcgaccaa agcccttcct tctgattgcc ataaacatgg
    12601 tcggatttcc cttctgggag tcccctctct aacgcaattg ctaccaacca gccgcttctg
    12661 gaaccttgtt caatataaga aaatcgctac tgacaggtaa gcatatagct tactgataat
    12721 aaacagcaga aaaaatatca ccacttatga aatttaaaat tgcatagcta tacccttatt
    12781 taaccaggca aaaaattgcg tagcagtgct ccagctactg ctttacaaaa ggtgtaacaa
    12841 taacaacatt aaccatttaa acaattaaca cataattaat tgaatggaaa ccttgaatat
    12901 ataaataatt ggaaaatttg aagaaaaata agtagccatg aatctacaca ggtacaccat
    12961 aaaaaaactg ctctccttca cctcaaccat catcctcctt tcatttcttc ttttcacctc
    13021 tatttactac attcgacagc aagcagtgcg gcaagataaa aacattcttc aaaggctaga
    13081 tcggctagaa caaaacagcc tgagaataaa aatgctgcag gatgaatttt tgctacgcga
    13141 cttccacaac agccagcttt acgtgctagg acaaagcccg gcaacgttga ggcttgacag
    13201 cctgtttaca aaaaccctcg aagaactcaa ctacctaaaa aaatatttta gctcgacaaa
    13261 gattaccgag gtcgagcggc acattgagca gtacaaaaag aacttcaaca acctgaagga
    13321 gctggagata accagaggtt tcagagacta tggacttgaa ggcgatctca ggaacatcat
    13381 ccaccaggtt gaagggaatg ccgttctgaa taatgactac aggcttctta gcggaatgct
    13441 catgctccgg cgtcacgaaa aggactacat gttgaggcgt gataccctgt atgttgagaa
    13501 attcgacgca acgctagcga gcacgcttaa ctatgctgct gataagtatg gggcaaacaa
    13561 ccagttttcg aagcagctga tagattacag caaacttttt catgagtttc tatccataga
    13621 tgcacaaata ggacgcacag aaaatgaagg agccacctac cagctacagc aacaatcggc
    13681 actggttatt gaaagcatta gaaatcttta tggcgaactt tcagaaaata tcaaaaagaa
    13741 agacagagag gcttatgcct ccatgctcat gttgctggtt atcgtgtcgt cgttggttct
    13801 tacgctgtta acaaaaataa gcaggcatat caccaaaacc atcaaatcaa cgctggctgt
    13861 aattaaaaaa ttggggaaag gagaacttcc cccaccactt gaagtaaagg gtgccgacga
    13921 gttttccgca atggagaaat ccatcaacga cttgagcctg gctctacgca acacccgtga
    13981 ttttgcaatt gaagtaggga atggaaactt ctacagcgag gtaaatgttt tcgggaactc
    14041 cggtgagtta ggatcaggcc tgctcgaaat gaggcagaag ctaaccgaag tggctgttca
    14101 gcaggaaaac agcttaaagg agaaggaaca gcgaagctgg ctcaacgagt gcctggcgca
    14161 ggtttcagaa ctactaagaa atttccattc cgacaccaag gagctttgct ttgagtttgt
    14221 ccgctttgcc attaaaagca caggttcgct tcagggtggc atatacctac tcaatagcga
    14281 aggtgagagt gatagtgatg aaaactcgta cctggaacta gtggcctcct acgcctacga
    14341 ccgacgcaag tatataaaca agcgatttga aaaatatgag ggagtagtgg ggtcttgcct
    14401 ctacgaaaag gatgtaatat acatcaccga tatacctgat agctacacgc acatcaccac
    14461 cggtttaggc gtttcgaacc ccagctgcat tgttcttacc ccactggtaa ccgaaaagga
    14521 agaggcgttg ggggttatgg agatttccag ctacagcccc ataaccgaca tacaggttga
    14581 atttatgaaa caagcatgcc gaaatctcgc ctcctcgttg attcttcata aggcaattaa
    14641 ggccaatgaa cagattatag cccaaatgaa ggtaaaaact gaggagctac tggccaacga
    14701 ggaagagcta aggcagaaca tggaggagct gaccgcaatc agggaggaca tggaacgacg
    14761 ggaaaaagac ctaaacggac aaattgccga gcttaaatac aagctaaggc ttcatgggat
    14821 tatttttgaa gccgatacct atcacaatta ggctgcacac gccagtgggt ggaggagtat
    14881 agacttttca actgcccagc gctaaaagta aatgcaacta gctaatttgc cagtcaactg
    14941 gttccttccc cgccctaacc aaaaaatcat ttgccctgga gaagtgcctg caaccgaaaa
    15001 aaccctggtt ggctgagaag ggcgatgggt gtggggcttt cagtatgcaa tgcttatgcc
    15061 catcaactaa tgccgctttg gcctgtgcgt agtttcccca tagcataaaa accaggtttt
    15121 cgcgttgctc ggccaatgct cttattgcgg catcggtaaa ctgctcccaa cccttatgct
    15181 ggtgcgaccc ggcctggttt gccctcacag taagggtagc attgagcaaa agaacacctt
    15241 gccttgccca ttcctccagg ttaccatgag gaggaatggc taaacccaaa tcgtcatgca
    15301 gctccttaaa tatgttcacc agcgatggag gcttaggtat gcctctgggg acagaaaaac
    15361 agagtccttg cgcctgcccc tgcccatggt agggatcctg acccagcagg acaactttca
    15421 cgctggtgaa tggggtaaag tcaaatgcag caaagatttt agagccggga gggtaaaccc
    15481 tgtactgcct ccgctcctca acaagaaaat tctttaactc cctgaagtac tcctgctgaa
    15541 actccccggc caaaaccttc ttccagctct cctctatctg cggatttatg ctaacatcgt
    15601 ccatggctac ctacctgaaa aacggaacaa gtgaattatc gaacccctaa aattactttc
    15661 aatttgtatt cttacaaaga attctcctca tctttcaata ttaagcctgc ttgcgatttc
    15721 gcagctttgc cacgttcatt ttacacatca agttcaacgt tggcttggca gccaatctac
    15781 tttttactat cttcgacgtc cgaattagaa tgggcagctt gaaggttttt gaaatttcaa
    15841 cctgcataaa tttccacctt aatgaagctt atagcttgta aaagacaatg tttttaatcc
    15901 tttctaaaca acttctacca tgaatgccga ccaacaaact agcgaacaat tctactggct
    15961 tgttcgaaac aggcagagcg acagggctta cagcaaccga ccggttgata gggaaatact
    16021 ggtgagggta gtggaagcag cccgtctggc tccatcggct tgcaatgctc aaccgtggaa
    16081 gtttattgtg gttgatgatc ctgagctaaa aaacaggctg gccgatgcag cagccagcaa
    16141 aatactgggt attaaccatt ttaccaagca agcgcccgtt cacattgtta ttgtgctgga
    16201 gggagctaac ctgaactcca actttggaag catggtaaag gggaaaacac taccgcttat
    16261 tgacattggc attgctgccg aacatatttg cctggcagct aaatcggaag gtttggggag
    16321 ctgcatactg ggttggttcg acgaaaaagc cgttaagcag ctactggata tacccaagtc
    16381 aaaacgcgtt ccgctcatca tcacgctagg ctatccaaaa accgaggaca taagggagaa
    16441 gcggagaaag ccgttggaaa aaattatatc atttaacggc tacagctaac tgtttaacag
    16501 ccaatcgctt ttccaacagc attcaggaga accggatact cgttgtccca gcatagtgtt
    16561 tcagaggccg ctaatacatt attaatcatg acctccctgg ccttcttgtt ttcaacaaag
    16621 tcggctacaa tgcccgccaa cttctccgga tctcttgtgt tggccactag tcctacatga
    16681 taggtctcaa caatttttcg catctcgggt aaatcgctaa ccaggattgg cacacctgag
    16741 gctatgtagt cgaaaatctt atttggcaga gcaaggcggt agttcaaccc caaatcctcc
    16801 tcaagcgata agccaagcca ggccctaccg gttacatcgg gtagcctgtc cggatcaact
    16861 ctgcccataa actcaaccct gtcggttagc ccccgctcaa tagctctccg cctaagaggt
    16921 atttctatat cgccacttcc aataaccagt aagtggtagc aggggagcca ttccatcatg
    16981 tcgatggcga gctctatgcc tctccccaca ttcaacgatc cttggtatat caacgttggc
    17041 ttcaaatccc tataaggaga cttatcacgc ttaaccgaaa agttacgaac aacaacgggc
    17101 ttaactccat acctggtgct cagctccttg gcaacaccct ctgaaactgt aatgacgcat
    17161 tttgcccttc gaatacatac ccgctcggca atggcccata aacgtttctt aacaggatgc
    17221 cctattagct ctggtatctc ggtaaaaagc tcatggctat cgtaaaccaa aggtttggct
    17281 tttaaaattg aagccagcca gcaccctgga agtgtatcca aatcgttgga gtatattaag
    17341 ctagtgcggg taaaaataag gcgggttaag attttaagat tgtactcgac ataaaacatg
    17401 ggaccatccg aaaacacaca acgaaaacgc tttactgcaa attcaaaagg tttttcatcc
    17461 ggtagtccat gcctcctgag ggcaaaaacg ttaacctgat acccattcct ggaaagcgtg
    17521 ctagctgctc gttgaatccg ctggtcggtt atcagatttg aggtagtaca aaaacttact
    17581 ttaatggctt tcatccggat aaatgtatgc tctatagtcc ataattccaa atcatacacc
    17641 ttttagcaag ctggcaaacc ccataatgtt tttcagcaag aaagctaaat gggaccatgc
    17701 tggaagacca ctactcaaca ttattcagaa tgaaatcgcg ttgagccttg agcaggttaa
    17761 cggtgtgcag catgatattc ttaatttcgt tgatgagccc gaggtacaga acgctgtttc
    17821 gggtaccggt tacaccctgc ttaattcgct taacctgatt ttttcttgcc tcgttaagct
    17881 tgtcaagaat gtcctgctgc ttgcttacaa cctgtggaac tccggtaaag tcgttattct
    17941 ttattatttt cattatctcc tgaaagaaat ccgaaacctg gttgctgatg gttgttaact
    18001 caatggcctg atcggtagtt agccccttgt gctggttctc aatgtgctta aggctgggct
    18061 cggttataaa cgagagtgaa tgggcaatct ccctgaggta atcaaccacc tgaacgtagt
    18121 agtggcctgt ttcgacagaa tcggcttcca gcttctttag tgtctgatag atgttgtttt
    18181 taaaattttt ggcattagaa ttaaggtcag ctacgcggtt gttgaccgtt ctaagcagct
    18241 tcctgttttc ggaggttaac ccgtcaatgg tttgggaaaa cacctcaacc acatctctta
    18301 gggagttgtt aacccccaat gtgcattttt caacaacact cttatcggta attagctctg
    18361 caccaacatc cctattctga tttctactgc ggtgaattaa gttcgacctg atgagcagcc
    18421 aaccggcaag aatggctaac cctaccaggg caaaagcttt ccagtagaaa agagcagcag
    18481 caacaacaaa cgatacgata aaggttatga gagctgtaaa gaaccaacct gcaatcacgg
    18541 taataacacc ggtaacccgg taaacagcgc tttcccttcc ccacgcccta tcgctgagcg
    18601 aggttcccat ggcaaccata aaggtaacat aggtggtaga aagcggtagt tttagggaag
    18661 ttcccaagct gatgagtatg ctggaaacca ccaggttaac agaagcacgt accaaatcga
    18721 aagcaggagc atccttgggg ttttttacca ctaaagcctt acttaaatcg aatctccggg
    18781 aaagaaatga tttaatgcgt agtggagtga ttctcccaac accttcagaa aaagaacggg
    18841 atatacgcac aatagacctg gagagctggg tgctgccaaa acgttcagcc ccgctatcct
    18901 ggttggccag tccaacttcg gtattggtaa cggttcgtgc cttacgggat gtccataggg
    18961 taatcaccat aaccaatcct gcaattagta ggtaaagcgt atttgttgag accggaccgg
    19021 ccaaatcggt catcagcagc tggtccggtg ccactccggg gtttgccagg tgtacctggt
    19081 aggttttgag cgccgccatg gtggggccaa taaagtttac caggtcgtta ccggcaaacg
    19141 aaagggctag ggcaaaagta cccgccaaca caacaaacct taggatattc catttgaaga
    19201 tggatatggc cagctgaaaa ataatggtcc acccggtaaa gctcaggagg agaatcacca
    19261 tggtgtgctg gttaataaag ttcagggtat ccggcgaaac cagggcggtg ctgttgagac
    19321 ccttcatggc aataaagtag gtaatggccg taagggagat tcctccccaa atactgccaa
    19381 cgtacttcat gctacgggtg tagttgaagg tgaaaagcac ccgggcaatg tactgcacaa
    19441 ttgtgccgat aaaaaatgct attaccaccg atataaaaat ccccgatatc atagccagcg
    19501 ccttggccgt gttgatgtag ctacctaaat cggcaatact ttcgttgttt tctgaaatct
    19561 taaaaattga aatggcaact gcggagccaa gcagctcgaa aaccagtgat accgtagtgg
    19621 aggtaggcag accgaaagta ttaaaaaaat ctagcacaat aacgtcagta agcatcacag
    19681 ccatgaacaa agtcatgatc tcgctcatgt aaaacttttc gggacggaac acccccttct
    19741 gggcaatctc catcattccg cttgaaaaaa ccgcgccaac cagcacccca accgaagcca
    19801 ccagcagaat ccaatggcgt gcggcaacac gcgatccgat agccgaattc agaaagttta
    19861 ccgcatcatt ggctacgcca accattaagt cggatattgc cagaacgaac agtaaaataa
    19921 ggattataag gtagtatatc tccatggtta aggagttaac aaacagttag caaagataaa
    19981 atagcccgtt aaaatggtta gccgtggggg attacgcttt tgttaagaca ttgttaagtg
    20041 aatattaaca attacccatt ttgtttgccg gctatcgccc tgagtatatc cctttttcga
    20101 accgaaacgg gcacttcatc gccgttgtcc atccttatgg taggtgaggg aaatcggaga
    20161 acttccttaa catgcctttt gttgataagg tgcgattgat ggcagcgaat aaaaccctca
    20221 ggagctaata gctcctcata ctccttcaga gttttagaca ccatgacacc ctgctcggta
    20281 gtaaggatga actcggtggt gttgttatgc gactcacaac gtacaatttc atcggtttct
    20341 acaacccgta gtccctctaa ggacttaaga acaagttttt tacatttttt gctacaggcg
    20401 cttgtgtttg agagtagcac ctgaaactta cggtttgcct cctgactcga aaacgagtgt
    20461 tgcagcctgt ccaccgcatc aaccaagctg ttaacatcta taggcttcag caggtagtcg
    20521 agggcacaat acttgaatgc cctaatggcg tattcctggt aggctgtaac aatgatgagc
    20581 atgaaatgca cctcctcaaa catacgaaga aggtcaaaac cattcccatc ggcaagctgc
    20641 aaatcgagaa aaactatatc cgggtttagc ttttgaatgg aggtgtaggc gctttcaact
    20701 ccttcagctt ctccaataac cctgactgtt gggcaaaaat ggcttagcag gtaggcaagc
    20761 gtctgccttg agttcagctc atcatcaact ataagggcgg taaacatggt taaatggaaa
    20821 ttttaaattt gaccagcgtg ccatgttgtt tgggatcaat actaccggtg tcaacaagcg
    20881 tcaagattat attcagcctt ttatagtgtc tgatgttttt aagcctctcc cgggttattt
    20941 cagttgcaag cgaaagatgg tctttcccag aagccgggtt tgcttgctcc gaccttttaa
    21001 tccccacacc attgtctttg acttcaattt caacatgatg cttatcgagg tagaggtagc
    21061 ttattgttaa tcttccccta tcttctatac cattaaatcc atgctcgatg gcattttcga
    21121 taaatggctg ggtgagcatg ggaggaatta gcgtgctgtc ggcatcaatt ttctcatcaa
    21181 cataaatgga gtagtcaaaa cggttgctga aacgcaggga ttgcagctcg aggtagtgct
    21241 cgagggttgc aacctcattt tcaattgagc atagctcgga gcgtgaattt tcgagtatta
    21301 ggcgggtaag cttggcaaaa ctagccaggt acttgctggc cagatgggcc tcgttgcgga
    21361 aaacgtagct ctgaatagca gttagagaat tgaaaacaaa atgaggattt agctgcgaaa
    21421 gcaaaagctg ttgctccagg cgaagatact tgcgctttag ccgtctatag gccaaaacaa
    21481 gcacaacagc taccatcgta atagctacgg taaaaatctc aaaaaaaagg cagccattca
    21541 tgcttatcct ttaaccattg ccccaactgg taaatcaatt acaactacaa aattagcagg
    21601 tagcatttag ttttcaaaaa aatttaaaaa tcacagatag actaatttac aacgaaccaa
    21661 taaaaaaccc ctacgcagtg caggggtagc ctaaaaaagg aacaaaataa tgctattgct
    21721 tggctttgtt gttatccatg tgcttcttgt agcggttcat aaatttgtca acgcgaccag
    21781 cagtgtcaac cagcttcatt ttaccggtgt aaaatgggtg tgaggtattg gatatttcca
    21841 cctttaccat tgggtattca actccgtcaa taactatggt atcctttgta tttgcagccg
    21901 acttggtgat aaaaacatga tcattcgaca tgtccttgaa tgccaccaat cggtaatttt
    21961 gcggatgtat tccctctttc atctgtttaa attttaatat tatgcagtgc tataatagtt
    22021 tacacgtaat attctactca agagactaac tgctcacctt ccaaatattc tcctaacatc
    22081 aacagcagcc ggacaattcc tcgtattttt cgggataaat ccatgctcgt ttcagcctgc
    22141 aaatgtacta atccggaatc aatatttcaa gtcaacacga acattttttc aaaaagttag
    22201 aaatttggat aggaaacgag ttgcgttaag aaataacttg ttgaaatgaa acacctatgt
    22261 aggacaaccg gcaaaaataa atgaacaaca gggcgttact ggcgtgctcg tcgcagccaa
    22321 gcaatgtgaa tttgcataag ggggacttca gggttcatcc gataacttcc gggattaaac
    22381 accaaatcgt atcgtattgt
//
LOCUS       DAOSHM010000038        22224 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_1876_length_22224_cov_20.262529, whole genome shotgun
            sequence.
ACCESSION   DAOSHM010000038 DAOSHM010000000
VERSION     DAOSHM010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 22224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22224
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1876_length_22224_cov_20.262529"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(50..2905)
                     /locus_tag="PK839_07150"
     CDS             complement(50..2905)
                     /locus_tag="PK839_07150"
                     /inference="COORDINATES: protein motif:HMM:NF019136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triple tyrosine motif-containing protein"
                     /protein_id="HOG72547.1"
                     /translation="MRFEVKFPAIHLHIILFVVFAFSLNRLQGQSALHITNYDKKEYR
                     AANQNWSVSLGGISAVFTGNNHGLLCYDGAEWLLKPVPGKSIIRAVLYDSSRVYVGLL
                     DEFGYFDIVNGKPTIYHCLSTTVSNNLKNQEYWRIAKHHGKIYFQSFGSLYVYDGREV
                     SSVPLDFPILLLIKANDELFTQRIGGGLYRITENGLKLLEGSEIFADTEIKAALTHQN
                     DILFCTNSMGIYRFNGFSFEPWHTAVDAELKTYNLNTAVETQHYYCIGTILKGVLVLD
                     HNGQLVYQLDSKHLQNSTILSLEKDKMGNIWVGKDKGISYVAFNSPVSIHIDDTDKFS
                     CYSGVLVGSYLYLGTNQGIFRYSFSSENMLTGKTLVTGSQGQVWFIKLIDGKLYCGLN
                     DGTYLLENDKLEKIGFVSGGYNLEKFSKEKDLLIQSTYNNLVTYRNNNGRWEQNKILQ
                     GFSSPSRFLQVDFQQNIWVGHSIVGVNRLQTNSNVDSIISIENIGQEHGLNFQTNRIY
                     KIENRIVIPTGSGFLRWNDLNERFEPFGELNAQLQGFEKAHAVASLPDDKYWLIKDDE
                     WGLFEIRFGKANLLYRVIPDMFNMELVEENEKIIQLNDSLQLVCLDNGFAILNILQLN
                     RLPEVNLPPNINDVRFWRNEKDSASIKPRMTRGMLISPAKFNNIRVTFASNEVIGTKR
                     YFQYTLEGMDGEWSTWQTSTSVSYKRLPPGDYTFKVRTLNSKGILTPAAMVKFSIQPP
                     FFLSWKAGILYICIILAISGISRNRYKKRIKQHFEQQQRQEQEKIQKEREENERVIMR
                     IQNERLRADIENKNSQLAMNTMSLIRKNALLQEFAAELENMKNELGYRIPNKYYDRIT
                     RLINQNIENEHDWETFQKLFDQAHENFFKSLKDAYPSLTTSDLRLCAYIRMNLSSKEI
                     APLLNITVRGVEERRYRLRKRLNLSTDESLNDFILKL"
     gene            3132..3626
                     /locus_tag="PK839_07155"
     CDS             3132..3626
                     /locus_tag="PK839_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HOG72548.1"
                     /translation="MSTKEQTLENDEKFMDEAIELAIKGVMSDEGGPFGAIVVKDGRI
                     VGKGNNHVTSTNDPTAHAEVMAIRDACRTLNTFQLNGCVLYTSCEPCPMCLGAIYWAR
                     LDKVFYSANRHIASYHGFDDSFIYNEVNLSFDKRRIPFINISPEKGLVPFREWEKKDD
                     KIKY"
     gene            3695..4606
                     /locus_tag="PK839_07160"
     CDS             3695..4606
                     /locus_tag="PK839_07160"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOG72549.1"
                     /translation="MLGLSKKGNTGVSLVLSSGGARGLAHIGVIEELLTRDFCINSVV
                     GSSMGALVAGLYASGGLENFKNWAVGLSKLEMLSLVDFTLTSRGIIKGEKVFNQMQKL
                     GLIPDINIEDLPIPFAAVAVDIINGKEVVFRSGSLLKAIRASIAIPSVFTPVAHEDGL
                     LIDGGVLSPLPLQHATRSKHDLLVAVDVNSFTPYGKPIARIRRRMEQVDKPKLLKKWD
                     AFNRQHKQPMSGRAMQKGVGYLQLLTRSSLLMQAKLTQYAVGESTPDILVPISKDVST
                     AYEFYRANELIGFGRQRCAEVLDGKGL"
     gene            complement(4601..5344)
                     /gene="rlmB"
                     /locus_tag="PK839_07165"
     CDS             complement(4601..5344)
                     /gene="rlmB"
                     /locus_tag="PK839_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008779732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HOG72550.1"
                     /translation="MDNQKDSPIYGIRPVMEALESGMKISRVVVKQGLSGSLVSELMV
                     LLKQRNVPVQYVPLEAFDKFKDKNHQGVVAFSSPVEFQNLEHILPKLLEREIKPFILM
                     LDGVTDVRNFGAIARTAECAGVDAIVVPAKGAASMGSDALKTSAGALNHIPVCRVGSL
                     KITINLLKQHGFRVIAATEKAQKSPFEANLTGAVAIVAGSEDTGISPDIIKLADELVR
                     IPIFGKIESLNVSVATGVVLYEVVRQRLL"
     gene            complement(5356..7314)
                     /locus_tag="PK839_07170"
     CDS             complement(5356..7314)
                     /locus_tag="PK839_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010264027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOG72551.1"
                     /translation="MISVNQLTLSFGGFELFKEISFQLNEGERVGLVGRNGAGKTTLL
                     KIIAGEMQPTSGRVSMPGSVSIGYLPQQMALPSGRSVMDETLMAFSETLELSRSIDKI
                     GEELSGRNDYESAGYLQLINRLSELTDRFNLLDGGTANAKAEQTLLGLGFRRSDFDRT
                     TGEFSGGWRMRVELAKILLRKPQVLLLDEPTNHLDIESIQWLEDYLKDYQGSLLLISH
                     DRAFLDNVTQRTVELSLGKLYDYKVPYSQYVELHRLRREQQMAAYRNQQKVIEDTNDF
                     IERFRYKATKSVQVQSRIKMLERMEIIEVDEDNTASVNIRFPSAPRSGDVVCEAKDVA
                     KSFGSKKIFGGVNFTIERGQKVAFVGRNGEGKTTMSRIITGELSHEGSLRIGHNVAIG
                     YFAQNQDELLDGSLTVLETIDRVAVGDIRTRMRDILGAFLFRGDDVDKKVSVLSGGER
                     NRLALAKLMLQPYNLLVLDEPTNHLDMHSKEVLKSALMQYDGTAILVSHDRDFLDGLV
                     GKVYEFSDGAVREHIGGIYDFLQRKKLESLKQLEHKARVDAQTTSSGNGSDLKRLWSE
                     RRELDREIRNMENSLKQVESKISAMENEIDDINRKLAEPDSHSINLSDSDFFAGYENK
                     KRQLLDYMQRWEELSLKLEELKLKRNAL"
     gene            complement(7475..8188)
                     /locus_tag="PK839_07175"
     CDS             complement(7475..8188)
                     /locus_tag="PK839_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019129698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HOG72552.1"
                     /translation="MEWIERTELLLGAAKLQKLSRAHVLVVGLGGVGAYAAEMICRAG
                     VGQLTIADGDTIHPTNINRQLVALNSTVGLPKAEYMGKRLLDINPDIKLTVVPKYLRD
                     DEMKDIINQPYSYVVDAIDTLSPKIFLIRDALEYGHRLVSSMGSGGKLDPTMVSIADI
                     SETHSCPLARILRKRLHRLGIRKGFKVVYSPEETPENATRPCVGEPNKKTTVGTISYM
                     PAVFGCFIASVVIRDLLAD"
     gene            complement(8279..8944)
                     /locus_tag="PK839_07180"
     CDS             complement(8279..8944)
                     /locus_tag="PK839_07180"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOG72553.1"
                     /translation="MPLINFHTHLTSQKEGEVRIVSLNVEEVDNIESLSMVTVGVHPW
                     QTDSNNMEDLLAKFHELIRHPNVIGIGEVGLDRLRGGSLTLQAKYLREQAIAAASISK
                     PLVIHCVRAWSELVETLSAPDLKQLTKAIHGFRGKADLARQLVDRNYYLSFGSILVDP
                     SPELAESLTVIPKNRLFFETDTSEMPIGEIYSAAADILDTSVDELTLQVENNLNTFFG
                     FNG"
     gene            complement(9005..10243)
                     /gene="pepT"
                     /locus_tag="PK839_07185"
     CDS             complement(9005..10243)
                     /gene="pepT"
                     /locus_tag="PK839_07185"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008144308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HOG72554.1"
                     /translation="MINKQSLIDRFLRYVAIDTQSDDTVSDRFPSTEKQLVLSNLLVK
                     ELKELGVSDATIDEFGYVMGTIPANSTKKVPVIGFLAHVDTSPDMPGKNVKPRFVESY
                     DGSDITLNAEKGVILSAREFPELKEYTGQTLIVTDGTTLLGADDKAGIAEIMTAVEYI
                     TGNPTLRHGKIRIGFTVDEEVGRGVDHFDVKRFGADYAYTLDGGAVGELEFENFNAAS
                     AKITIQGRNIHPGYAKDKMLNAILIAQEFNSMLPPHESPEHTEGYDGFYHITKIEGTV
                     EKASFQYIIRDHDMRKFERRKAYMERVTRYLNSKYGDQTVTLEINDQYYNMREKVEPV
                     YHVVQTAIEAMEKVGIKPKVRPIRGGTDGARLSYMGLPCPNLFAGGENFHGKHEYVPV
                     ESMVKATETILKIVEIYESR"
     gene            complement(10489..13758)
                     /locus_tag="PK839_07190"
     CDS             complement(10489..13758)
                     /locus_tag="PK839_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HOG72555.1"
                     /translation="MKKVCSMMLLLGLLSVGLAGAQEARLMRFPAVHGNQVVFTYAGD
                     LYTVPLNGGVARKLTSHIGYEMFARFSPDGKTIAFTGQYDGNTEVFTIPAEGGTPKRL
                     TYTATLSRDDISDRMGPNNIVMTWTPDGKNIVYRSRKQSYNDFVGSLFMVPIDGGLSS
                     ELPLTGGGFCSYSPDGKQLAFNWVFREFRTWKYYKGGMADDIRIFDFDTKQITNITNT
                     NTQEIFPMWIGGKIFFLSDRDRTMNLFCYDLKTQETKKVTNFTDYDIKFPSFSGNTIV
                     FEKGGYLYSLDASSLAANKIDVTIADDQLYSRSELVDAGKSIRSMDLSPNGERVVFSA
                     RGDVFTLPAESGITRNLTQSSDAHDRNAAWSPDGKYIAYISDKSGEYEIYLQAQDGAS
                     APVQLTTNTNNYIFNIEWSPDSKKILFTDRLSRLQCVDVETKAVSQLAKGKYGSIYSY
                     SWSPDSKWVAYSTEDENRFSVVFLYNTETASTYNVTGNWYTSTNPVFSNDGLYLLFVS
                     DRDFNPIYSSTEWNHAYTDMSKIFMVMLSKNTPSPFAPENNEVKIEPKPVTDAKKKNA
                     DDKKATEAQPTVKPVQIDIDGIVDRTVSLPIDAANYRVAACIDGKVYYSYYGSGRGSQ
                     VARVYDLKKKTETDLGESLQFAISANGKKMLVSKAGKYSVIDLPSSKVNMEKTISLNS
                     MKVWVDYRQEWKQIFDESWRQMRDFFYVENMHGLDWKAIHDKYAVLVPYVNHRNDLTY
                     IIGEMIGELSIGHAYVNSGVKPEPERTNTGLLGAKVSKDASGYFKIDRILEGASWDKS
                     LRSPLADIGVNVKTGDYILAVDGADLKNTPDIYAKLIDKADKTVELTVNSKPELNGSR
                     KVLVVPVANEANLYYYSWVQNNIRKVSEATNGQVGYIHIPDMGVEGLNEFVKYFYPQL
                     TKKALIIDDRGNGGGNVSPMILERLSRVAYRQTMRRGISYASPIPSETHVGPKVLLID
                     RYSASDGDLFPYGFKKLKLGPVIGVRTWGGVVGISGSLPFVDGADLRKPEFASFSSDA
                     SEWIIEGHGVDPDIVVDNDPYQEYMGVDTQLNKAIEVALDKLKDFKELPPIPPAPDKS
                     K"
     gene            14057..17107
                     /locus_tag="PK839_07195"
     CDS             14057..17107
                     /locus_tag="PK839_07195"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HOG72556.1"
                     /translation="MRHIGYFLLAATAALALPSSSNAVGLKNCHSGNGSAEKKIQETW
                     ADSVLSSLTLRERIGQLFMVAAYSNRDDMHVREIADLVRKDGVGGLIFFQGGPVRQAN
                     LTNYYQSLAEVPLLVGMDAEWGLGMRLDSTFSYPRQMMLGAAQDSALVYEMAADIARQ
                     LKRMGVHINFAPVVDINSNPQNPVINTRSFGENVNLVSRYGKAYVSGLQDNGVMACAK
                     HFPGHGDTFEDSHFSLPKVPHSKQQLDSVELYPFKQLISYGVQSIMVGHLRVPAMEND
                     TLLASSLSPAIISGNLISEMGFKGLIFTDALNMKGVADYFSPEDINLKALLAGNDVLL
                     FPSEVSRSIDKIEKMVRRGQIPEEVINIKCRKVLMAKYAAGLSSYKPIEVLNLYSDLN
                     RVSSEVIRRKIISLALTVVHNNNSFIPVKRLDTLRIAYLEIGCNRGDAFREQLELYTA
                     IHTFSIDPSSPAEEFDSLLNILEPYNLVIAGYHATDSRFSMNYGVTNQAANFLFDLAF
                     SRAVVLDIFGSPYAISKFYNPESFRAIIVSYDNSFDAQNLSAQLIFGGRFATGRLPVT
                     ASAWYPAGYGVTTINPIRLGYALPEEVGINSLLLKEVDSLANSAIELAVAPGMQILAA
                     KDGVVFYHKSFGKPTYLSSDSVDVRMLYDVASVTKVVATVPSVMRLYDEQALELDNNL
                     GAYLDLKEFPDKSAIRLSDLLRHQSGLKSWIPFNLKTLTTMIPNIPLWQEKLSTDYPF
                     QLGSKQFFSRYTVPNPVFYSDKESRDFPNRCAEGLYSAYFMADTIFSAINRSELSQPG
                     KYVYSDLGFMYLQRVVEGITQTGIDRYCEDKFYSRLGMSYTLFKPLTRFDRDRIVPTE
                     VDLIFRRQLVWGDVHDPAAAMLGGVSGHAGIFSTANDLAKLMQMFLQDGTYGGERFFR
                     PGTVAQFTTAPIEGNSSDNRRSLGFDKPASTGPSPAGSLASSQSFGHTGFTGTTVWAD
                     PENGLVYIFLSNRVYPDASNTKLAEMNIRTRIHDVFYRAIGVAK"
     gene            complement(17267..20086)
                     /locus_tag="PK839_07200"
     CDS             complement(17267..20086)
                     /locus_tag="PK839_07200"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HOG72557.1"
                     /translation="MKKTRFYLCALGILLGFSAGAQDQVNWNTPIPLDRNVRFGQLEN
                     GLTYYIRKNTEPQQRAEFFIAQNVGAILEEDNQNGLAHFLEHMAFNGTKHFPGKGIIN
                     YFETVGVKFGFNINAFTSLDETVYYLSDVPTNREGIIDSALLVLHDWSGFISLEPDEI
                     EAERGVIREEWRTGRNAERRMYKQVMPVIYKGSKYAERDVIGDINVINNFKHEEIKNY
                     YHKWYRPDLQSIVIVGDVDVDQVEQKIKTLFADIPKRENPDVRPFFELPDNDEPLIGI
                     ASDPEARNTILTVYYKHQAVPKNEKNLGYVREQMIQKLATSMISARLNEITQKPNPPF
                     VFAASDMGNMVRTKSALMFYAALRNDAMIDGIKGLIREAERVKQFGFTATEFDRAKSD
                     YMRGLENELKEKDKQKNQKYVWEAINNFLEGEPMPGIEFEFMLANGLVPTITLEEINA
                     YIASMVTDKNVALTLMAPKKDDIQLPSEEAILAAVKEVKSETLQAYVDNVVMKPLVES
                     IPTPGQIKKVNDKNIFGTTELTLSNGVKVILKPTDFKEDEVKLDAFSKGGLSLADLAT
                     LPSATLATDMVVNAGVGEFSSVDLEKLLAGKMVRVNPVFGEDFEGFQGSASPKDFETM
                     LQLVYLYMTAPRQDQENFNTYLDRLKAFFANAAADPRMAFRDSVNTAMANHNPRVMPI
                     NTDFLSKVDFNKCYSFYRDRFADASDFTFIFTGNINVDEAKALTAKYLGALPVVKRKD
                     IPRDNGVRPPKGKVQNYFTKPMQTAKSSVFVGFTGNAEYNLKNHVMAEYLTSILRTRY
                     LEEIREKEGGTYGVGVNISIDNFPTQSFTAGIQFDTDPKLRDKLIGIVYAEIEKIKQN
                     GPTPDDLNKTKEFLMKEYEQNQRENSYWSDAIRMKYEDGVDFSTEYLNVVKSTSVESL
                     KDFAQKLFNQNNVVEVVMTSEEAQK"
     gene            20559..21752
                     /locus_tag="PK839_07205"
     CDS             20559..21752
                     /locus_tag="PK839_07205"
                     /inference="COORDINATES: protein motif:HMM:NF024241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOG72558.1"
                     /translation="MKKFLTRTIVMLSLVSLFTDIASEMLYPIMPVFLKSIGFSVLLI
                     GILEGLAEATAGISKGYFGHLSDRLGTRVPFIRWGYTLSAISKPLMAVFTWPIWIFGA
                     RTLDRLGKGIRTSARDALLSDESTRETKGKVFGFHRGMDTLGAAIGPIAALIYLHFYP
                     QNYSTLFLIAFAPGLVAVSLTLLLKDKKKPETSNQQVNPVQGKGFFSFVKYWGHSPKA
                     FRYLVVGLLFFTLFNSSDAFLLLMVKEKGFSDANMISLYIFYNLLYALLAYPMGWLAD
                     KFGMKRIMIFGVSVFGLVYLLFGFADKLHMFLLLFGLYAFYAAATEGVSKAWITNIVP
                     GESTATAIGFYTGFQSVATLLASSMAGLIWTKFGASTVFVISGLAAFITAIYLMLATR
                     MNKLA"
     gene            complement(21744..22208)
                     /locus_tag="PK839_07210"
     CDS             complement(21744..22208)
                     /locus_tag="PK839_07210"
                     /inference="COORDINATES: protein motif:HMM:NF025491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4293 domain-containing protein"
                     /protein_id="HOG72559.1"
                     /translation="MIQRIQSLYLLISAILLAFFFFMPYAHYAVGPQEVKISLSASGL
                     GQDGSVVENLPKLWAVLALVILAFTATLVTIFLFKKRKLQARLCIFNIVLLIGLQGLL
                     FYIAKAVEQRLMAPPSYGLIFIFPLVCAIFTYLAYRAIAKDEALIRSLDRLR"
BASE COUNT         5401 a         5270 c         5373 g         6180 t
ORIGIN      
        1 gttcttgaat cctgcttgaa tttttttatc ctttatcctt taaccttctt tagagcttga
       61 ggatgaaatc gttcaggctt tcgtcggttg agaggttcag ccttttccga aggcggtacc
      121 tccgctcctc cacaccacga acggtaatgt tgagcagggg ggcaatctcc ttcgacgaga
      181 ggttcatccg gatgtaggcg caaagccgta aatcgcttgt ggtcaggctc gggtaggcat
      241 ccttcaggct tttgaaaaag ttctcgtgag cctggtcaaa cagcttttga aaggtttccc
      301 agtcgtgctc attctcgatg ttctggttga tgagcctggt tatacggtca tagtacttgt
      361 tgggaatgcg gtatcccagc tcattcttca tgttttcgag ctcagcggca aactcctgca
      421 gaagggcatt ttttctgatg agggacatgg tgttcatggc cagctgcgag ttcttgtttt
      481 cgatgtcggc ccgtaagcgc tcattctgta ttcgcatgat tacccgctcg ttttcctcgc
      541 gttccttttg aattttctcc tgctcctgtc gttgctgctg ctcaaaatgc tgctttatcc
      601 ttttcttgta acggttgcgt gaaatgcctg aaatggccag aatgatgcag atgtagagta
      661 ttccggcttt ccacgaaagg aaaaacgggg gttgaatgga gaatttcacc atggctgctg
      721 gcgtcagaat tcccttggag ttcaacgttc taaccttaaa ggtgtaatcg ccggggggca
      781 ggcgcttgta gctcaccgag gttgatgttt gccaggtgct ccattcgcca tccataccct
      841 caagcgtgta ctggaagtag cgtttggttc ctatcacctc gtttgaagcg aaggtaaccc
      901 tgatattgtt gaattttgcg ggagaaatca gcattcccct ggtcatcctg ggcttgatgc
      961 tggcgctgtc cttttcattc ctccagaatc gcacgtcgtt gatgttgggg ggaaggttga
     1021 cctcaggcag cctgttgagc tgaagtatgt tgagtatggc aaaaccatta tcgaggcaaa
     1081 ccagctgaag gctgtcgttt agctggatga tcttctcgtt ctcctcaaca agctccatgt
     1141 taaacatgtc gggaataacc cggtaaagca ggttggcctt gccaaagcgt atttcgaaaa
     1201 gcccccattc gtcgtcctta atcagccagt acttgtcgtc ggggaggctg gcaacggcgt
     1261 gagccttctc gaaaccctga agctgggcgt tgagctcgcc gaagggctcg aaccgctcat
     1321 tcaggtcgtt ccacctcaaa aatcccgaac ccgttggaat tacaattcta ttttcgattt
     1381 tgtagatgcg gttggtttga aaattcaggc catgctcctg gccaatgttc tcgatgctga
     1441 tgatgctgtc aacattgctg ttggtttgca gccggtttac ccccacaatg gaatgcccca
     1501 cccagatatt ctgctgaaag tcaacctgaa gaaatcggct gggcgatgag aatccctgaa
     1561 ggattttgtt ctgttcccac ctcccattgt tatttcggta ggttaccagg ttgttgtagg
     1621 tgctttggat gagcaaatcc ttttccttgc tgaatttttc gaggttgtac ccgccgctta
     1681 caaagcctat cttctccagc ttgtcgtttt caagcaggta ggtgccgtcg tttagcccgc
     1741 agtaaagctt cccgtcaatc agcttaataa accaaacctg gccctgcgaa ccggtaacca
     1801 gcgttttgcc ggtgagcatg ttttcgctgg agaaggagta gcggaatatt ccctgattgg
     1861 ttcccaggta gaggtatgag ccaaccagaa cgccggagta gcacgagaat ttatcggtgt
     1921 cgtcaatgtg gatgctcacg ggtgagttga aagccacata gcttataccc ttatccttac
     1981 caacccagat gttgcccatt ttatccttct cgagcgaaag aatggtgctg ttctgcagat
     2041 gcttggagtc cagctggtaa accagctgcc cgttgtgatc cagaaccagc acgcccttta
     2101 gaatggtgcc aatgcaatag taatgttggg tttcgacagc ggtgttcagg ttgtaggttt
     2161 tgagttcggc atccacggca gtgtgccagg gttcaaagga gaacccgttg aacctgtaaa
     2221 tgcccatgga gttggtgcaa aataggatgt cgttttgatg ggtaagggca gccttaattt
     2281 cggtatctgc aaatatctcg ctgccttcca acagcttaag accattttcg gtaatgcggt
     2341 ataacccgcc accaattcgc tgtgtgaaaa gctcatcgtt tgccttaata agcagaagta
     2401 tcggaaaatc aagaggaacg gaggaaacct cccgtccatc gtaaacgtaa agtgagccga
     2461 aactctgaaa atatattttc ccgtggtgct tggctatccg ccagtactcc tgatttttaa
     2521 ggttgttgct taccgtggtt gacaggcaat ggtaaatggt tggctttccg ttgacaatat
     2581 cgaagtagcc gaattcatcg agcaagccga cgtaaaccct gctgctatcg tagagcacgg
     2641 cacgaataat ggatttcccg ggaactggct taagcagcca ttcggcacca tcgtaacaga
     2701 gtaaaccgtg gttattgccc gtaaaaacgg cagatatacc tcctaggctt accgaccagt
     2761 tttggttggc agccctatac tcctttttat cgtagttggt tatgtgtagg gccgattgcc
     2821 cctgtagccg atttaagctg aatgcaaata ctacaaaaag aataatatga agatggattg
     2881 ccggaaattt tacctcgaag cgcatacgtt tgaaattgga tcgtaacaaa gttagaactt
     2941 attacctaaa agcaaagcat acgctgaaaa ttcagagggt aatttttaag ggctaactta
     3001 tccgtagagt tcaattttat gctgctgaca gtattgaaag gggctgaatt ttccccaaaa
     3061 ctccctgccg cctttgtggg gttggataat ttgttatatt tttacaggtt gaacaaaaat
     3121 gaagattata gatgagtact aaggagcaaa cgctggagaa cgacgagaaa tttatggatg
     3181 aagccattga gctggcaatc aagggggtga tgtccgatga gggtgggccg tttggggcaa
     3241 tagtggttaa ggatggcagg attgttggca aaggcaacaa ccatgtaacc tccaccaacg
     3301 atcctaccgc ccatgccgag gttatggcga taagggatgc ctgccggaca cttaacacct
     3361 ttcagctgaa cgggtgcgtg ctttacacct cgtgcgagcc ttgtccgatg tgtcttggag
     3421 cgatttactg ggctcgtctc gataaggtgt tctactcggc caaccgccat attgcctcgt
     3481 accatggttt cgacgatagc ttcatttaca acgaggttaa cctttcgttc gataaacgac
     3541 gcattccctt tatcaacatt agccccgaaa aggggttggt tcccttcagg gagtgggaaa
     3601 agaaggatga taagattaag tactaacccg ttagcccatt ttgttccggc agcaggttgg
     3661 ggttgtataa atgcaccatt atgagtggca ggcaatgtta gggctgtcga aaaaaggtaa
     3721 cacaggtgtt tccctggtgc tctcgagtgg tggagccaga gggttagcac acattggggt
     3781 tattgaggag ttacttaccc gggacttttg tatcaactcg gtggttggct catcaatggg
     3841 tgccttggtg gctggtttgt acgcctcggg agggcttgag aattttaaga actgggctgt
     3901 tggactcagt aaacttgaga tgctctcgct tgttgatttc accctaacct ccaggggtat
     3961 cataaagggt gaaaaggttt tcaaccagat gcaaaagctg gggttaattc ccgacatcaa
     4021 catagaggac ctgcctatcc cttttgctgc cgtagcggtt gacatcatta atggcaagga
     4081 ggtggtgttc cgtagcggta gtttacttaa ggctattcgt gcttcaattg caattcctag
     4141 tgttttcact ccggtagcac atgaagatgg gctgctgatt gatggaggcg ttctttcgcc
     4201 tcttcccctc cagcatgcaa ctcggagtaa gcacgacttg ctggttgccg ttgatgtgaa
     4261 ctcgtttact ccgtatggca agcctatagc taggatcaga aggagaatgg agcaggttga
     4321 caagcctaag ctgttaaaga agtgggacgc attcaaccgt cagcataagc aacccatgtc
     4381 cgggagggct atgcaaaaag gggttggcta cctgcagcta cttacccgct cgtcgctgct
     4441 aatgcaggcc aagttaaccc aatatgccgt gggtgaatcc acacccgaca tactggtgcc
     4501 catttcgaag gatgtttcca cagcttatga gttttacagg gccaacgagc tcatagggtt
     4561 cggacggcaa aggtgcgccg aggtgctcga tgggaagggg ttatagtaag cgttgcctaa
     4621 ccacctcgta gagcactacc ccggtagcca ccgaaacgtt tagcgattca attttcccga
     4681 atatgggtat tctcaccagc tcatcggcaa gcttgataat atcggggcta atgccggtat
     4741 cctctgaacc cgctactatg gcaactgccc ccgtaaggtt agcctcaaag ggagattttt
     4801 gggctttttc ggtagcagcg ataactctaa aaccatgttg tttcagcagg ttaatggtta
     4861 tttttaggct gcctacccgg cataccggga tatgattcag cgcaccagcc gaggttttta
     4921 gggcatcgct acccatgctt gcagcaccct ttgctggcac tacaattgca tccactcctg
     4981 cacattcggc agttcgggca attgcgccaa aattccgaac atcggttacc ccgtcgagca
     5041 taagaataaa tggttttatc tccctttcaa gcagcttggg aaggatatgc tcaaggttct
     5101 gaaactctac gggcgacgag aaggccacaa ccccctggtg atttttatcc ttaaacttgt
     5161 cgaacgcttc aagcgggacg tactggacag gaacattcct ttgctttagc agcaccatca
     5221 gctccgacac caggctgccg ctcaacccct gctttacaac taccctgctt attttcattc
     5281 ccgattccag agcttccatc accggacgaa taccgtagat tgggctgtct ttttgattat
     5341 ccatagctgc cagtgttaaa gtgcatttcg tttcaacttc agctcctcca gctttaggga
     5401 gagctcctcc cagcgctgca tgtaatcaag aagctgcctt tttttatttt cgtagcctgc
     5461 aaaaaagtcg gaatccgaca ggtttatgct gtgtgagtcc ggttcggcca gtttgcggtt
     5521 aatatcatca atctcattct ccatggccga tattttgctt tcaacctgct tcaggctatt
     5581 ttccatgttt cgtatttcgc gatcaagttc cctgcgctcc gaccatagcc ttttaagatc
     5641 cgatccattg ccggagctgg tagtttgagc atcaacccga gctttgtgct ccagttgttt
     5701 caggctttcc aacttcttac gttggaggaa atcgtagatt cccccaatgt gctcacgcac
     5761 agcaccatcg gaaaactcat aaaccttgcc tacaagtcca tccagaaaat ctctgtcgtg
     5821 tgaaaccagt attgccgttc catcatactg catcagggcc gatttgagca cctccttcga
     5881 gtgcatgtca aggtggttgg ttggctcgtc gaggacaagc aggttgtagg gttggagcat
     5941 caacttagcc agggccaggc gattacgctc accaccggaa aggacgctga cttttttatc
     6001 tacatcatca cccctgaaaa ggaaagcgcc aagaatatcg cgcatcctgg ttcggatgtc
     6061 cccaaccgca accctgtcaa tggtttcgag aacggtgagg gagccgtcga gcagctcatc
     6121 ctgattctgg gcaaagtagc caatggctac attatggcct attctaagtg agccctcatg
     6181 gctaagctcg ccagttatta ttcggctcat ggtagttttt ccctccccat ttctaccaac
     6241 aaaagccacc ttttgcccgc gctcgatagt gaagttcacc ccgccaaaaa tttttttgga
     6301 accaaagctt tttgccacat cttttgcctc acaaaccaca tcgcccgagc ggggagcaga
     6361 ggggaagcgg atgtttaccg aagcagtatt atcctcatca acctcaataa tctccatcct
     6421 ttcgagcatt ttgatgcgcg actgaacctg aacggacttg gtggccttgt agcgaaaacg
     6481 ctcaataaaa tcattggtat cttcaattac cttttgttga ttccggtaag cggccatttg
     6541 ctgctcacgc cttagcctgt gaagctctac atattgggag tagggaactt tatagtcgta
     6601 cagcttgcca agtgaaagct caacggttcg ttgcgttaca ttgtcaagga aagccctgtc
     6661 gtgcgaaatg agcagcagcg atccctggta gtccttaagg tagtcctcca gccattgaat
     6721 agactcaata tcaagatggt tggtgggttc atcaagcagg agaacctgtg gtttgcgaag
     6781 tagaattttg gcaagctcga cccgcattct ccatccaccg ctaaactcgc cggttgtcct
     6841 gtcgaaatcg ctacgcctaa atcctaaccc aagtaacgtt tgctcggctt tagcattggc
     6901 cgttccgcca tccagcaggt taaacctgtc ggttagttca gacaagcggt ttatcagctg
     6961 caggtagccg gcagactcgt aatcgtttct tcccgagagt tcctcaccta ttttgtcgat
     7021 gctcctactc agctcgaggg tttcggaaaa tgccataagg gtttcgtcca taacgcttct
     7081 gcccgatggc aatgccattt gctgggggag gtatccaatg ctaacgctgc ctggcatcga
     7141 aacgcgtcct gaggttggct gcatttcgcc tgcaataatc tttagtaggg tagtttttcc
     7201 tgcgccgttt cggccaacca acccaacccg ttcgccctcg ttaagctgaa agctgatttc
     7261 cttgaaaagt tcaaaacctc cgaagctgag ggttagctgg tttactgata tcatctgaga
     7321 ctactcgaaa agttattggc tgcaaagata ctaatttcgc ttcaccctgc catcaatggc
     7381 attattgtgc cctccagacc gtgaagctgt ggatgtgcag ttgtgatgat gttgatggag
     7441 gaggagtgat tgcgttaacc acaaccattg tgggttagtc ggccagtagg tcacgtatca
     7501 ccaccgaagc aataaagcac ccaaaaacag caggcatgta agagattgtg ccaacagtag
     7561 ttttcttatt tggttcaccc acacatggtc ttgtggcgtt ttcgggcgtt tcctcagggg
     7621 agtaaaccac cttaaatccc ttacggatac ccaacctgtg gagccgtttg cgaagaattc
     7681 gggcaagcgg gcagctgtgg gtttccgaaa tatcggcaat ggaaaccatg gttggatcaa
     7741 gttttccacc gctacccatt gagcttacaa ggcggtgtcc atactccagg gcgtcgcgaa
     7801 tcaggaatat tttaggtgag agggtgtcaa tggcatccac cacgtagctg tagggctgat
     7861 tgatgatgtc cttcatctca tcgtcgcgca ggtacttagg caccaccgtg agcttaatat
     7921 cagggtttat atccagcagg cgttttccca tgtattctgc tttgggcagg ccaaccgtgc
     7981 tgttcagggc aaccagctgc ctgttgatgt tggtagggtg gatggtatcg ccatcggcta
     8041 ttgtaagctg cccaactccg gcgcgacaaa tcatttcggc agcataggcg ccaacaccgc
     8101 caagcccaac caccagcaca tgcgcccggc ttagcttctg cagcttagca gctccaagca
     8161 ggagttcggt tcgctcaatc cattccattg ctgtaagttg gtagagtgct cataaagttg
     8221 ctacatttcc tcgggtgtga ggcaaggggt gttttagccg ggtaatttta catggcaatt
     8281 agccattaaa cccgaaaaac gtattcaggt tattctcaac ttgcagggta agctcatcaa
     8341 ctgaggtgtc gaggatatct gcagcggcgg agtatatctc cccaataggc atttcggagg
     8401 tatcggtttc aaaaaacagc ctgtttttgg ggataacggt aagcgattcg gccagctcgg
     8461 gcgatgggtc aaccagaatt gacccaaaag agaggtagta gttcctgtca accagctgcc
     8521 tggcaagatc tgccttgccc ctgaatccat gaatggcctt tgtgagctgt ttgaggtcgg
     8581 gtgcagaaag ggtttcaacc agctcgctcc atgccctaac acagtgaatg actaaaggct
     8641 ttgaaatgct agctgctgct attgcttgct cccgaaggta ttttgcctgc agggttaaac
     8701 ttccaccgcg gagccgatca agcccaacct cgccgatacc tatcacgttg gggtgtcgta
     8761 taagctcatg gaacttggcc agtaaatcct ccatgttgtt gctatcggtt tgccatggat
     8821 gaacaccaac cgtaaccatg cttagcgatt caatattatc aacttcttct acgttgagcg
     8881 aaacaatccg tacttcgcct tccttttggc ttgtgaggtg ggtatgaaag tttatgagcg
     8941 gcattctgct tacaggataa atgttgttta cggttgaagt gtatgcgggt tgaaacttaa
     9001 atatctatct tgactcgtaa atttcaacaa ttttcagtat ggtctcggtg gccttcacca
     9061 tgctttcaac cgggacatac tcgtgttttc catgaaagtt ttcaccgcct gcaaacaggt
     9121 ttgggcaagg gagtcccatg taggaaagcc tggcaccatc ggttccaccg cgtatgggtc
     9181 gaaccttagg cttaatacca actttttcca ttgcctcaat ggcggtctgc accacgtggt
     9241 aaacaggttc caccttttcg cgcatgttgt agtactggtc gttaatctca agggtaaccg
     9301 tttggtcgcc gtacttactg ttaaggtacc gggtaacacg ctccatgtat gccttgcgtc
     9361 tttcaaactt cctcatgtca tgatcgcgga tgatgtactg aaatgaagct ttctcaacgg
     9421 ttccctcaat tttggtgata tggtagaaac catcgtagcc ctcggtgtgc tcggggctct
     9481 cgtggggcgg taacatgctg ttgaactcct gggcaataag aatggcgtta agcattttat
     9541 ccttagcata gcccggatgt atattccggc cctgaatggt aatcttggca ctggcagcgt
     9601 tgaagttctc aaactccagc tctccaacag caccaccatc aagcgtatag gcatagtcgg
     9661 cgccgaaacg tttcacatcg aagtggtcaa ccccccgacc aacctcctcg tcaaccgtga
     9721 agccaatacg aattttacca tgtcgtaaag ttggattgcc ggtgatgtac tcaacggccg
     9781 tcatgatttc agctattccg gccttatcgt cggctccaag cagggttgtg ccatcggtaa
     9841 ctataagtgt ttgcccagta tactccttga gctcggggaa ctccctggcc gatagaataa
     9901 cccccttttc tgcattcagg gtaatgtcgg agccatcgta gctctcaaca aagcgtggct
     9961 tgacattttt cccgggcata tcgggcgagg tatcaacatg tgccagaaag cctataacag
    10021 gaactttctt tgttgaattg gcaggaatgg tgcccataac gtagccaaac tcgtcgattg
    10081 tcgcatcgga tacgcccagc tcctttaact ccttcaccag caaatttgat agaaccagct
    10141 gcttttcagt gcttggaaac cgatcggata ccgtatcgtc gctttgcgta tcaatagcta
    10201 cgtaccttaa aaaacggtct atcagggact gcttatttat catggcttga gtatgttaaa
    10261 aagcaaattt gttttttaga aaaataatga cgtcttagaa aaaatagcct ttaaatttag
    10321 gttctttggg acaactatgc ataaaaaaat gaaaataatt tactcgttta tgctagtagg
    10381 ttgataaata gaaggttata gccatgctgg aagttgatag tagttaaaaa acaaagcggc
    10441 tcatgttaag ccatggccgc tctgtagttt tgtatcttgt tgttccggct acttcgattt
    10501 atcgggtgct ggtggaattg gtggcagctc cttaaagtcc ttcaacttat caagtgcaac
    10561 ctcaatggcc ttattcagct gggtatcaac gcccatgtac tcctggtagg ggtcgttgtc
    10621 aaccacaata tcggggtcaa caccatgtcc ctcgataatc cactcgctag catcgctgct
    10681 aaagcttgca aattccggtt tacgcaggtc ggcgccgtca acaaatggaa gtgagccgga
    10741 aataccaaca actccacccc aggttcgaac cccaataacc gggccgagtt tcagcttttt
    10801 aaatccgtat gggaataaat cgccatcgga agctgagtag cggtcgatga gcagcacctt
    10861 aggcccaaca tgtgtttcgg atgggatggg cgaggcgtag cttatacccc gccgcatggt
    10921 ttgccggtag gcaacacggc taagacgttc aagaatcatg ggcgaaacat tacctccgcc
    10981 gttacccctg tcgtcaataa tcagcgcttt cttggttagc tgggggtaga agtactttac
    11041 aaactcgtta agcccctcaa cgcccatgtc gggaatgtgg atgtagccaa cctggccatt
    11101 ggtggcctcg gataccttgc gaatattgtt ctgaacccag ctgtagtagt acaggttggc
    11161 ctcgtttgcc acgggaacaa ccaacacctt ccggcttccg ttcaactcgg gtttgctgtt
    11221 caccgtcagc tcaaccgttt tatcggcctt gtcaatcagt tttgcgtaga tgtcgggggt
    11281 attcttcagg tcggctccgt caactgccag aatgtagtcg cctgttttaa cattaacgcc
    11341 gatatcggcc aggggcgaac gaagggactt gtcccagctg gcaccctcaa gaattctatc
    11401 tattttgaag tacccgctgg catccttgct cactttggcg ccaagcaacc cggtattggt
    11461 gcgctcgggc tcaggtttta ccccgctatt tacataggca tggccaatgc tcagctcccc
    11521 aatcatctcg ccaatgatgt aggttaagtc gttgcgatgg tttacatatg gaaccagcac
    11581 ggcatactta tcgtgtatgg ctttccaatc cagcccgtgc atgttttcaa cgtagaagaa
    11641 atcgcgcatc tgacgccagc tctcgtcgaa aatctgcttc cactcctgac ggtagtcaac
    11701 ccacaccttc atgctgttga ggcttatggt tttctccata ttcaccttcg aagagggtaa
    11761 atcgataacg ctgtactttc cggccttgga taccagcatc ttttttccat tggccgatat
    11821 ggcaaactga agcgattcgc ccaggtcggt ttcggttttc ttctttaagt catataccct
    11881 cgccacctgc gaacccctgc ccgaaccgta gtagctgtag taaaccttcc cgtcaataca
    11941 tgcggcaact ctgtagtttg cagcatcaat agggagtgat acggttcggt caactatccc
    12001 gtctatatca atttgaacag gttttaccgt gggttgcgct tcggtagcct ttttatcgtc
    12061 ggcatttttt ttctttgcat cggtaaccgg ttttggctca atcttcacct cgttgttttc
    12121 gggagcaaag ggtgagggtg tgttctttga gagcatgacc atgaaaattt tgctcatgtc
    12181 ggtgtaggcg tggttccatt ccgtggagct atatattggg ttgaagtccc tgtccgaaac
    12241 aaaaaggagg taaagcccat cgttggagaa gacaggattg gttgaggtgt accagttgcc
    12301 cgtaacgttg taggttgaag ccgtttcggt gttatagagg aacaccacgc tgaagcgatt
    12361 ttcatcctcg gtggagtagg ctacccattt gctgtcgggc gaccacgagt agctgtatat
    12421 tgagccatat ttccccttag caagttggct aactgccttg gtttcaacat caacgcactg
    12481 caaccttgag agcctgtcgg tgaaaagaat ttttttgcta tcgggcgacc actcaatgtt
    12541 gaatatgtag ttattggtgt tggttgtaag ctgcaccgga gccgaggctc catcctgtgc
    12601 ctggagatag atttcgtact caccgctttt atcggagatg taggcaatat actttccgtc
    12661 gggcgaccaa gctgcattgc ggtcgtgggc gtcggagctt tgagtaaggt tgcgggttat
    12721 tccgctctcg gcgggaagtg tgaacacatc acctctggcc gagaatacca cgcgctcgcc
    12781 gtttggcgac aaatccatgc tgcgaatgct cttgccggca tccaccagct cgctgcggct
    12841 gtaaagctga tcgtcggcaa tagtcacgtc aatcttattg gctgccaggg aactggcatc
    12901 aagggagtaa aggtatcccc ccttttcaaa aacgatggtg ttgcctgaga atgacggaaa
    12961 tttgatatcg tagtcggtaa agttggttac ctttttggtc tcttgggttt tgaggtcgta
    13021 gcagaacagg ttcatggttc tgtccctgtc ggagaggaag aaaattttac cgccaatcca
    13081 catggggaaa atctcctggg tgttggtgtt ggtgatgttg gtgatttgct tggtatcgaa
    13141 atcaaaaatt cggatatcgt cggccattcc acctttgtag tacttccatg ttcggaattc
    13201 gcgaaaaacc cagttaaagg caagctgttt tccgtcgggc gagtaggagc agaatccccc
    13261 tccggtgagg ggtagctcac tggacaaacc gccatcaatg ggaaccatga atagcgaacc
    13321 gacaaaatcg ttgtaggatt gttttcggga acggtacaca atgtttttac cgtcgggtgt
    13381 ccatgtcatt acgatgttgt tggggcccat acggtctgaa atgtcatcac gcgaaagggt
    13441 tgccgtataa gtaagccttt taggtgttcc accttcggca ggaatggtaa atacctcggt
    13501 gttaccatcg tactgcccgg tgaaagcaat tgtttttcca tcgggcgaga aacgtgcaaa
    13561 catctcgtag ccaatgtggc tggttagctt gcgagccaca ccgccattga gtggcacagt
    13621 atataaatca cccgcatagg tgaacaccac ctgatttccg tggactgccg gaaaacgcat
    13681 taacctggcc tcctgggcac ctgctaatcc aacggacagc aaacccaaaa gaagcatcat
    13741 gctgcaaact tttttcatct tgaaattatt ttagttgaag ttatggttgt gttcctgtaa
    13801 ttactcatgt tcgatagggg aatgattgtt tggtttaatc agctcatctc aaagccttga
    13861 tagtatgact aactaacgtc gggcagtgcg aaacctttaa atttagaaat gtaaatctct
    13921 cagcaggaat tactttacaa gtacctaaaa tttttgcaga tttcaaaacc ctaaagcaaa
    13981 atgttttcat gcactgcttg tccctgtgaa aatgacgtaa aacctgctac ttttgttgca
    14041 tcataaatac taggtgatga ggcatatcgg ttattttctt ttagcagcaa ccgcagcgtt
    14101 ggcattgccg agcagtagta atgccgttgg tttgaaaaat tgccattcag gcaatgggtc
    14161 tgcagaaaaa aaaatacagg agacatgggc tgattcggtt ttgtcatcgc ttacgctgag
    14221 ggagcgaata ggccagctat ttatggtggc tgcatactcc aaccgcgacg acatgcatgt
    14281 tagggaaatt gccgatttgg ttaggaagga tggagttggc gggttgattt tttttcaggg
    14341 agggccggta aggcaggcca atcttaccaa ctactaccag tcattagccg aggtgccgct
    14401 acttgtgggc atggatgctg aatgggggct tggcatgcgg ctcgatagca ccttctccta
    14461 tccccgccag atgatgcttg gggctgctca ggattccgct ttggtttacg aaatggctgc
    14521 cgatattgcc cggcagctga aacgaatggg tgttcacatc aactttgcac cggttgttga
    14581 tatcaacagc aatccacaaa atccggttat caatacccgt tcgtttggag aaaacgttaa
    14641 cctggttagc cgctatggca aagcctacgt gtccggatta caggataatg gggttatggc
    14701 atgtgccaag catttccctg ggcatggaga tacctttgaa gattcacact tttccctgcc
    14761 aaaggttcct cacagcaagc agcagctcga ctcagttgaa ctttacccat tcaaacagct
    14821 tatcagctac ggagtacaaa gcattatggt tgggcaccta agggtacccg ctatggagaa
    14881 tgacacctta ctggcctcca gcctatcacc tgccattatt tccggtaacc ttatttccga
    14941 aatgggattt aaggggctga tcttcaccga cgccctgaac atgaaggggg tagccgatta
    15001 ctttagccct gaggatatta acctgaaagc cctgctggcc ggtaacgatg tgctgctttt
    15061 tccatccgag gtgagccggt caattgataa aattgagaag atggtgcgta gaggacagat
    15121 acccgaggaa gtcatcaaca tcaaatgccg aaaagttctt atggcaaagt acgctgcggg
    15181 acttagcagc tacaaaccca ttgaggtact aaatctttac agcgatctca acagggtgtc
    15241 ctcggaggtt atacgtagga aaatcataag ccttgcgcta acagtggttc ataataacaa
    15301 tagctttatc cctgtaaaaa ggctcgatac gctcaggatt gcctacctgg agatagggtg
    15361 taaccggggt gatgcattta gggaacagct ggagctttac accgcaattc atacctttag
    15421 tatcgatcct tcttcgcctg ccgaagagtt cgattcgctg ctgaacatac tcgagccata
    15481 caacctggta atagccggct accatgctac tgatagccga ttttctatga actacggagt
    15541 aactaaccag gctgctaatt ttctcttcga tttggccttc agcagggcag ttgtcctcga
    15601 catatttgga agcccatatg ccatttcaaa attctataat cccgaatcat ttcgtgccat
    15661 tattgtttcg tatgacaact cgtttgatgc tcagaatctt tcagcccagt tgatttttgg
    15721 aggccggttt gctactggga ggctgcctgt taccgcaagt gcctggtatc ctgccggata
    15781 tggagtaaca actatcaacc ccattcgctt aggttacgcc ttgcctgagg aggtaggcat
    15841 aaactcactg ctgcttaagg aggttgactc cttggccaat tcagctatcg agcttgctgt
    15901 tgccccggga atgcaaattc tggcagctaa ggatggcgtg gtgttctacc ataaaagttt
    15961 tggaaagccc acctatcttt ccagcgattc ggttgatgtt cgtatgctct acgatgtggc
    16021 atcggtaacc aaggtggtgg caacagttcc atcggtcatg aggctctacg acgagcaggc
    16081 acttgaactt gataataacc tcggagccta cctcgattta aaggaattcc ccgacaagtc
    16141 agcaataagg ctatccgacc tgctcaggca tcaatcggga cttaaatcat ggataccttt
    16201 caacctgaaa acccttacca caatgattcc caatatacca ctatggcagg agaagcttag
    16261 caccgattat ccgtttcagc ttgggagcaa gcaatttttc agccgctaca cagtgccaaa
    16321 tccggttttt tacagcgata aggaatcgag ggattttcca aaccgctgtg cagaaggact
    16381 atactctgcc tacttcatgg ccgataccat ttttagcgcc attaaccgct cagaactctc
    16441 ccaacccggc aagtacgttt atagcgactt agggtttatg tacctgcagc gggtggttga
    16501 gggcataacc cagacgggta ttgaccgata ctgcgaggat aaattctaca gccggttggg
    16561 aatgagctac acgctcttca agcctttaac ccgttttgac cgggacagaa ttgttccaac
    16621 ggaggtggat ttgattttcc gacgtcagct ggtatggggc gatgtgcacg atccggcagc
    16681 agccatgctg ggcggggtat cgggacatgc cggcatcttc tccactgcca atgacctggc
    16741 taagctaatg cagatgttcc tgcaggacgg aacttatggg ggtgaacgct ttttccgtcc
    16801 gggcactgtg gcgcaattca ccacagcccc aattgagggc aacagcagcg ataatagacg
    16861 ctctcttgga tttgacaaac ccgcatcaac gggtccatca ccagccggaa gtttggcctc
    16921 ttcacaaagt tttggacaca ccggatttac cggaacaacc gtctgggctg atccggagaa
    16981 tgggttggtt tatattttcc tttcgaacag ggtttacccc gatgcttcga ataccaagtt
    17041 ggccgaaatg aatatccgca ccagaattca cgatgttttt tacagggcta ttggggtagc
    17101 aaaatgaggg gtagccaact cacaatctga aaaatgaaag attgcgaagc atggtgtacg
    17161 ctcaaagtag ccccttaaat tatctgttct ttatggaata ataaatgact aaaaagaaaa
    17221 gacgcctgtt taagcgtctt ttctccattt caagtactag ctgttgctat ttttgtgcct
    17281 cttcggatgt cataacaacc tcaaccacat tgttttggtt gaatagcttc tgtgcaaagt
    17341 cctttagact ctccacgctg gttgatttca ccacgttgag gtattccgta ctaaaatcaa
    17401 cgccatcctc atacttcatc ctaatggcat cggaccagta gctgttttct ctttggtttt
    17461 gctcgtactc cttcatcagg aattcctttg ttttattaag gtcgtcggga gtagggccat
    17521 tctgcttaat cttttcaatt tcggcataca caattccaat gagcttatcg cgaagcttgg
    17581 gatcggtgtc aaactggatg ccggcagtaa atgactgggt tggaaagtta tcaatgctga
    17641 tatttacgcc tacgccgtag gtgccgcctt ccttttcacg aatttcttca aggtagcgtg
    17701 tgcgaagaat agaggtaagg tattcggcca tcacatggtt tttgaggtta tactcggcat
    17761 taccagtaaa tcctacaaac actgatgatt tagcagtttg catgggtttg gtgaagtagt
    17821 tttgcacctt tcccttgggc ggtctaacac cgttgtccct gggaatatcc tttcgcttaa
    17881 ctacaggtag tgcaccaaga tacttggctg tgagcgcttt agcttcatcc acgttgatgt
    17941 tgccggtaaa aataaaggtg aaatcggaag catcggcaaa ccggtcgcgg tagaagctgt
    18001 agcacttgtt gaaatcaacc ttactcagaa aatcagtatt tatcggcata accctagggt
    18061 tatggttagc catggcagtg ttaacactat cacggaaagc cattctggga tcggcagcgg
    18121 catttgcaaa aaatgccttt agcctgtcta aatacgtgtt gaagttctcc tggtcctgac
    18181 ggggagccgt catgtaaagg taaaccagct ggagcatggt ttcaaaatcc tttggagatg
    18241 cgcttccctg aaatccttcg aaatcctctc caaataccgg gttaacgcga accattttac
    18301 ctgcaagcag cttttcgagg tcaacgctcg aaaactcacc cacgccagca tttacaacca
    18361 tatcggtagc cagggtagca gatggtagcg ttgctaaatc ggccagcgac aagcctccct
    18421 tgctgaaggc atccagctta acctcgtctt ctttgaagtc ggtaggttta agaattactt
    18481 taactccgtt ggagagcgta agctccgtgg tgccaaagat gtttttgtcg tttaccttct
    18541 ttatttgacc tggagtagga atgctttcaa ccagaggttt catcaccacg ttatcaacgt
    18601 atgcctgcaa agtctcggat tttacctctt taacagcagc cagaatggcc tcctcgcttg
    18661 gcagctgaat atcatccttt ttcggtgcca taagggttaa cgcaacattc ttatcggtaa
    18721 ccatactggc aatgtaggcg ttgatctctt caagggtaat ggtaggaaca agcccgtttg
    18781 caagcatgaa ttcaaattca atgcccggca tgggctcccc ttccagaaag ttgttaatag
    18841 cctcccaaac atacttctga ttcttctgct tatccttttc cttcagctcg ttttctaacc
    18901 cgcgcatgta gtccgattta gccctgtcga actcggtagc cgtgaaacca aactgcttaa
    18961 cccgttcagc ctcgcgtatt aatcccttaa tgccatcaat catggcgtcg ttacgtagag
    19021 cagcatagaa catcagggcg cttttggtcc gaaccatgtt ccccatatcg gaagcggcaa
    19081 aaacaaaggg agggttaggt ttctgggtaa tttcattcag ccgggcagaa atcatggaag
    19141 ttgccagctt ttggatcatt tgctcccgaa cgtagccaag atttttttcg tttttgggaa
    19201 ctgcctgatg cttgtagtaa acggtgagga tggtgttgcg ggcttcgggg tcggacgcaa
    19261 tgccgattaa cggctcgtcg ttatcgggta actcgaagaa aggtctaacg tccggattct
    19321 cccgtttggg tatgtcggca aacagcgttt tgattttctg ctcaacctgg tcaacatcaa
    19381 catcgccaac aatgacgatg gattgtaaat cgggacggta ccatttgtgg tagtagtttt
    19441 taatttcctc atgcttaaag ttattgatga cgttgatatc gccaataaca tcgcgctcgg
    19501 cgtatttcga tcccttgtaa atcaccggca tcacctgttt atacatcctt cgttcggcat
    19561 tacgtccagt gcgccactcc tcgcggataa ctccacgttc agcttcaatt tcatcgggtt
    19621 caagcgagat gaatcctgac cagtcgtgca gcaccagaag ggcgctgtca attattccct
    19681 cgcggttggt aggaacatcg gacaggtagt aaacggtttc gtcaagcgag gtaaaggcgt
    19741 tgatgttgaa gccaaactta acgccaaccg tttcaaagta gttgattata cccttacccg
    19801 gaaaatgctt ggtgccgttg aaagccatgt gctccagaaa atgtgccaga ccgttctggt
    19861 tatcctcctc aagtattgca cctacattct gggcaataaa aaattctgcg cgctgttggg
    19921 gttcggtgtt cttcctaatg tagtaggtta ggccgttctc cagctggcca aaccgaacat
    19981 tcctgtcaag aggaatgggg gtgttccaat tcacctgatc ctgagcccca gcgctaaaac
    20041 ctagtagaat acccagggcg catagataaa atcgtgtttt tttcattacc ggatgtttaa
    20101 gttaattttt tgttattagt aaaaatttat atcaaaggta gccaaatttt atgttacata
    20161 aactaaacgt tcacttttta acatattttg aaactttata gctggattaa gccctggttc
    20221 aataagtatt tttctaaagt gttcattttg cccggtttct ctggcctggt ttatatatgc
    20281 aaaatcccaa caatcaggat tatccttatg ctttccaatg gacttttatc ttgtatttgc
    20341 caacctgttc aaatgcctgt ttcctatgcc aacaattaaa atttctgcag ctgaatatca
    20401 aggcaacatt tgtttgaata actttccggg ctgttcggaa aggatggcaa catggttaaa
    20461 taattgaatt attgttcttt atttttttat tcatagtaag ttctgaggtt tagctagctg
    20521 ctaattattc tttggatatt taatttaacc tactctgcat gaaaaagttt ctaacccgta
    20581 ccatcgtgat gctttcgctg gtaagcctgt ttacggatat agccagcgaa atgctttacc
    20641 caataatgcc ggtttttctc aagtccattg ggttttcggt tttgctgatt ggtattcttg
    20701 aagggttggc tgaggccaca gctggaataa gcaagggcta cttcgggcat ctttccgaca
    20761 ggttggggac gcgagtaccc tttataaggt ggggctacac ccttagcgcc atctccaagc
    20821 ccctgatggc cgttttcacc tggccgattt ggatatttgg tgctcgaaca ctcgacaggc
    20881 ttggaaaggg catacgaacc agcgcccgcg acgccctgct cagcgacgag tccaccaggg
    20941 aaacaaaggg gaaggtattt gggtttcatc ggggcatgga tactcttgga gcagctattg
    21001 ggcctattgc tgcgcttata tacctgcact tctaccccca aaactacagc acgcttttcc
    21061 tgatagcctt tgctccaggt ctggtggcag tttcgcttac cctcttacta aaggataaga
    21121 agaaaccaga aacaagtaac caacaggtta atcctgttca gggtaaggga tttttttctt
    21181 ttgtaaagta ctgggggcat tctcccaaag cttttcgcta cctggtggta ggcctgctgt
    21241 tttttaccct tttcaacagc tccgatgcct ttctgctcct catggttaag gagaaagggt
    21301 tctctgatgc caacatgatt tcgctctaca tattctacaa cctgctctat gcgctgcttg
    21361 cctaccccat gggttggctg gccgataagt ttggcatgaa gcggataatg atctttggcg
    21421 tatcggtgtt cggcctggtt tacctgctct ttggatttgc agacaagcta cacatgttcc
    21481 tgctgctctt tggattatat gcattctatg cagccgcaac cgaaggcgtt tcaaaggctt
    21541 ggataactaa cattgtgcct ggcgaaagca cggcaaccgc tataggattc tacaccggat
    21601 tccagagcgt ggcaactctt ttagccagca gcatggccgg gttaatctgg actaagtttg
    21661 gagcatcaac tgttttcgtt atttcgggct tagcggcatt cataaccgcc atctacctga
    21721 tgctggcaac cagaatgaat aagttagcgt agccggtcaa gcgagcgtat aagggcttcg
    21781 tccttagcaa ttgcccggta ggcaaggtag gtgaaaatgg cgcaaaccaa ggggaaaatg
    21841 aatatcaagc catacgatgg gggtgccatt agcctttgct ccacagcttt agcaatataa
    21901 aatagcaagc cctgcaagcc aatcagaagc acgatgttga aaatgcacaa ccttgcctgc
    21961 agttttcttt ttttaaagag gaatatggtt accagggtag cggtaaaagc cagtatgacc
    22021 aaagcaagga ctgcccaaag tttaggtagg ttctccacta ctgaaccatc ctgacctaaa
    22081 cccgatgcgc taagcgaaat tttaacctcc tgtggcccaa cagcgtaatg ggcgtatggc
    22141 atgaaaaaga aaaatgccag taaaattgca gatattagga ggtaaagcga ttgtattctc
    22201 tgtatcatgg ctgaatatgc ttaa
//
LOCUS       DAOSHM010000039        22213 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_1877_length_22213_cov_19.253272, whole genome shotgun
            sequence.
ACCESSION   DAOSHM010000039 DAOSHM010000000
VERSION     DAOSHM010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 22213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22213
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1877_length_22213_cov_19.253272"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(62..2932)
                     /gene="gcvP"
                     /locus_tag="PK839_07215"
     CDS             complement(62..2932)
                     /gene="gcvP"
                     /locus_tag="PK839_07215"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HOG72560.1"
                     /translation="MTTDNFVARHNGPRDHEIDEMLKEIGVSTLDELINQTIPQNIRL
                     RKPLNLPNPVSEYEFLGRMKKIAAKNKLYKSYIGMGYYGTVTPSVILRNVFENPGWYT
                     SYTPYQAEISQGRLEALLNFQTMVIDLTRMEIANASLLDEATAAAEAMIMMHNLRSRE
                     AVKAGKNILFVDRDIFPQTLAVITTRSKPLGIDVVTGCYSEYSFTGREFGVVIQYPSA
                     KGNIRDYSSFTAKAHEAGTLVTVMADLLSLALITPPGEWGADIVAGSTQRFGIPMGFG
                     GPHAAYFACKDEFKRSMPGRIIGVSIDRHGNKALRMALQTREQHIKRERATSNICTAQ
                     ALLATMAGMYAVYHGPSGIRRIASYIHSVAMLVAKQLQLLGYTQNIKNIFDTLEVTLP
                     AGVSLEDIRRNALENELNLNYKPNGSIGISFDEVTTINDINALLNVFAKAIGKEHNPI
                     SSVEEANGIPANLQRRSPYLTEKVFNSYQSETEMMRYIKKLERRDIALNHSMISLGSC
                     TMKLNPAATMLPLSWPEMANIHPFVPADQAAGYHEIISELEHLLAEITGFDSVSLQPN
                     SGAAGEYTGLMVIREYHLSHGDSKRNVVLIPSSAHGTNPASAAMAGMQVVVVACDEMG
                     NINVDDLKAKAEQYRETLSAFMVTYPSTHGVFESKIREMVDIIHKNGGLVYMDGANMN
                     AQVGLTSPGEIGADVCHLNLHKTFAIPHGGGGPGVGPIAVAKHLTKFLPSHPVVKTGG
                     ENGISAVAASPWGSASILPISYGYILMLGAEGLTQVTKMAILNANYMAAKLKKHFKIL
                     YTGETGRVAHEMILDCHHFKTTYGVDTSDVARRLMDYGFHAPTVSFPVHESLMVEPTE
                     SESKQELDRFIEAMISILAELEDIKAGKNATPTDNLVLNAPHTAPEVSADEWHHPYSR
                     QKAAYPLTWIASNKFWPYVSKIDSGYGDRNLVCTCAPIEDYL"
     gene            complement(3048..3686)
                     /locus_tag="PK839_07220"
     CDS             complement(3048..3686)
                     /locus_tag="PK839_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOG72561.1"
                     /translation="MKVKVFVFNPFQENTYVISDENHNAAIVDAGCYNATEVALIKRF
                     VDDNELKVKYFVNTHCHVDHLLGLNLLKSIYNVEAYAHSDDLPLLKTFPQQAMSFGFS
                     VDKTPEIDKIVADGNTLLLGDGSIGIIHTPGHTPGSICLYLQNEKMLLTGDTLFYRSI
                     GRTDLPGGSYDAIIRSITTKLMVLPDDVAVYPGHGESTTIGDERRSNPFIKT"
     gene            complement(3932..4585)
                     /locus_tag="PK839_07225"
     CDS             complement(3932..4585)
                     /locus_tag="PK839_07225"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HOG72562.1"
                     /translation="MQQDTYKHKGLRKRLIEEIESKGITDKQVLGAMLRVPRHFFMES
                     SFLKFAYSDQAFPIGYGQTISQPFTVAFQTQLLQVEHMNKVLEVGTGSGYQAAILMEL
                     GVRLFTIERQRELYLNAKSLLEEMGYHPSFHFGDGYKGLPGYSPFDRIIVTAGAPTIP
                     DELVDQLAVGGRMVIPVGDENGQVMTLVEKISSTEVRKTTHGNFIFVPLLKGVEKSK"
     gene            4833..5696
                     /locus_tag="PK839_07230"
     CDS             4833..5696
                     /locus_tag="PK839_07230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72563.1"
                     /translation="MLKLGFFTDNTQLAELVGDLGRQANIEVEGIWAEPLLNGDDKRN
                     FSNCFSDPEELYGSIDVGVISITSSELFMDIVTAAIKNGVHPLVVGLPDLPLSTYNQL
                     SLLSAEMGVELGFSHIYHRFSGFNYPLVSPIIAHLTRGITMNQLNQFEMAATLRFDIA
                     TILSLTTSEVRRIRTYVLPQSGECPSCMMVFIDFFNNSAITYNLTLGAKRNFLELRLN
                     SIGEAIELYEDEIKLATSSLGGMISTSIFLDDLNNFTGCIGIQREPLLGISFVMKILS
                     AVEAISQKMAR"
     gene            5752..7167
                     /locus_tag="PK839_07235"
     CDS             5752..7167
                     /locus_tag="PK839_07235"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOG72564.1"
                     /translation="MTKNYLKIWYILFDVVGSAAAWTLFCIYPAKDVGLEFLRAGATF
                     EFDGKFYASLVFFVLFWLALFIVAGFYNNLYRKSRLKEFTKSVGYTFVGTVVIFFILL
                     LDDSLATYNDNFSIIGSMFILELTFTYFPRLVLTTGTIRKIHRRIIGFPTIIVGNGFK
                     ALQVYRELEAEKKSQGFRFVGYIAQVNNHEDMLQSQQKRLGEMDSIAEVVKKQGVEEV
                     ILAVEGEDVSTLTRCINELLPLKVNLRAIPSMYDFLLGRVKMTGILNAPLISVSFDLM
                     PLWQANIKRIADYTVSALAIVLLLPVSLILGIAIRCTSQGPIIFKQERIGQYGRPFWI
                     YKFRSMYADAEKSGPTLSGKDDPRVTPIGRFMRKTKLDEIPNFINVLKGEMSLVGPRP
                     ERQCYIEQIVRIAPHYVHLQKVKPGITSWGQVKFGYAKNVDEMVERLKYDILYLENMS
                     LFVDLKIIIYTILTIFKGKGL"
     gene            complement(7164..7730)
                     /gene="thpR"
                     /locus_tag="PK839_07240"
     CDS             complement(7164..7730)
                     /gene="thpR"
                     /locus_tag="PK839_07240"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HOG72565.1"
                     /translation="MKKERTFIAVGIKPEAKLLNCWHKLKQQHNLDFINWVDEGMLHL
                     TLRFLGEIPVDLVGDVSKVLSISLTGFNSFSLKVKELGSFGRPTPRVIWVGIEQNETL
                     NNLKQTIDKALLPLGFDREKGLFMPHITLGRVNFIRNPRAFYDMTEKLQNYTFQESEI
                     EEVTFYQSLLTPKGAIHKPIKNFLLLKG"
     gene            7815..8471
                     /gene="elbB"
                     /locus_tag="PK839_07245"
     CDS             7815..8471
                     /gene="elbB"
                     /locus_tag="PK839_07245"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF008747.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HOG72566.1"
                     /translation="MANSKKFAVVLSGCGVLDGSEIHEAVMTLYAITLSGSSYEIFAP
                     DVNQHHVVNHLNGSEVDQTRNVLVESARIARGKIKPLGDFSSDVFDAIIFPGGFGVAK
                     NLSSWAFDGAGCSVNADVANAVRSMHRLHKPIGALCIAPVVIARILGGVTVTIGKDAA
                     TVKEIETLGARHQMALQTQVVVDEKNMIFSTPCYMLDATIVDIAEGAKAIVSEMLKWI
                     "
     gene            complement(8484..9110)
                     /locus_tag="PK839_07250"
     CDS             complement(8484..9110)
                     /locus_tag="PK839_07250"
                     /inference="COORDINATES: protein motif:HMM:NF025492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4294 domain-containing protein"
                     /protein_id="HOG72567.1"
                     /translation="MRSSRINMTKSKLLPLAILLGITLSSGHSHAQVRDSVVVNLYTI
                     VFYEGDTIPYIKLREVVKYGKRRFKSRSEMNQYYKMIRNLKLTYPYAQLAKYKLLEMN
                     EDLKMIKTERERREYINQAEKELRMQYEKDLRKFTVSQGKMLMKLIDRETGATSYDLV
                     KELKGGFSATFWQGVARLFGSNLKVKFDPEGEDKLLNELIGLYEKGLL"
     gene            9470..12511
                     /locus_tag="PK839_07255"
     CDS             9470..12511
                     /locus_tag="PK839_07255"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOG72568.1"
                     /translation="MKKTLLTLVLITWGWVLSAQEITVTGTVISASDGIPLPGVSVTV
                     KANPSIGTATGIDGRYSIKVDANATLLFSFVGMETKEMPVNGQTIINVSMVEATQVLD
                     ELVVVGYGIQKKSLLTSSVAKVGSDEIAKTNPLRVEQALQGKTAGVQIISNSGQPGDG
                     LTVRIRGIGTNGTSDPIYLVDGMPVGGIDYLNPNDIESVQVLKDASATAIYGARGANG
                     VVLITTKSGSKGKMAVTYDYSFGLQNAWRKVSLLDASEYALIMNESYANDNAAIPFAD
                     VASLIDTVGSGTDWLDELFYKNAPVQNHQFSVSGGNDNSSFYSSFSYTTQDGIVAKGK
                     SNYERYTYRINSNHQYGKFGFGNNLSYSNKKNRGIDPNAEFGGPLAKAVNMDPLTPVK
                     YADGTWGVSSYAAQEVVNPVAFLSVLHSDYTENKLVGNVWGELELVKGLKVKSNFSID
                     YANGNSRTFTPVYDLGGNIKTLVSEASVTENRWFTWQNENTLSYSNQIDLHSFTAMVG
                     MTANKYHHEFVGGRKTDLLFDDFEHAWINNGTDEESYRAWGGADEHALLSYFARVNYN
                     FNEKYLLEAVLRADGSSNFGSNNRFGYFPAFSAGWILSKEDFLQGFTPLSFLKVRAGW
                     GRNGNEAIGAFKYTSIIGSGSKYTFGSDEVITVGSNPSGIPNPDLKWETSEQYNIGID
                     SRFFNERLNFTLDLYNKKTKDLLVVAPIPGYIGNAAPTVNGGTVTNKGVELELGYKTS
                     INKLDVDVSLSGAYNKNEVTDINNAEGKIYGAGLAVGMSNICMMQEGFPIAYFWGYKT
                     AGVFQNEAQILSYISSDSTVIQPNAKPGDLIWVDKNDDGKIDDNDRMLIGNPYPDFTA
                     GLNVNLAYRNFDFNMFWYGAFGQDIFDGTRRYDLPMSNWNASVLDRWTGEGTSNSNPR
                     VTIADPNKNYVNVSDFYVHNGSYLRLKNLTLGYTIPTTISQKVKIQRMRVFMSVSNLL
                     TFTSYKGFDPEIGAKSALDVGIDRNIYPQSRTFMFGVNLNF"
     gene            12531..14117
                     /locus_tag="PK839_07260"
     CDS             12531..14117
                     /locus_tag="PK839_07260"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOG72569.1"
                     /translation="MTKIKYFAVTLVLLLGMGSCSQDFLEMEPLTDRVEGNFYKTEKD
                     AFEALVSIYDVLQWQSSYGYHPWDLVSNVLSDDAFSGGSGPGDRPGLNRMGKFSSYTT
                     DEEALGLWKDRYSGIYRANIFLEKIDGIQFKDEALKDRYIAEARFLRGYFYFELVQFY
                     GHVPLILKTLTTSEYKQSQANPADVYNQIASDLLFAWEKLPGNISAAEYGRVSKWAAG
                     AMLARVYLFHKGYGKGVLGITSDLTVDNVPITESNIEQIVDDIINLSGHALVPNFADL
                     WGVNHENNKESIFEIQHTDKSNWGDWGWRNGTEGNWTVVMSGFRDVSDPIYDLGWGFQ
                     PASQSLANEFEPGDPRFSVSILDAAAENLVYKPQNCYQHTGYAFKKYYPKKADKPLSN
                     PDLNWGYNRVVIRLADVLLMGAELYLDNDLLKAQGYYEQVRKRAFGQSHVAPTLTNDQ
                     NGLDLIYHERRVEFAGEGIRYWDLLRRGLNYAQQKIDAASSASPYDETFKPATKGLLP
                     IPQSEISISGNILVQNEGYI"
     gene            14142..15914
                     /locus_tag="PK839_07265"
     CDS             14142..15914
                     /locus_tag="PK839_07265"
                     /inference="COORDINATES: protein motif:HMM:NF012999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOG72570.1"
                     /translation="MSKKNILFGVLVLVGLTWHACEPSEVGKPDVGKAPRVEDLSFTI
                     TPGEDAFHFVFTNTSSLKGIANWDLGNGSKAIGNTVTAYFPIAKTYTVTLTLYASGGS
                     ASLSQELTQNETDWDFLASPLITAITGGVESANGKTWVIDSINPGHLGVGPANETKPS
                     WWSAQPREKTGHFLYDDEFTFKLVDFVYGIDTHGKTHVNHDGNADEDGFAGGYYTSLL
                     WNDAYDADVATNDAARGALTWSINEVNGKSYINISSQSGNISYDDGNPRSYEILEWND
                     NFLYLRSASAGNARYHKLIPKGYVKPKISYELSITPTLNPNEYSMQLSNVTIPADLTI
                     SKVEWDFGDASSPNVYTSTDYNEVVTFTYMAAGTYTVKVTVTDNNHDTYVVTSQVVVA
                     ADHPDYVPYIETGMSIYANFDDIFCKFAVDNADNVGSFSLIANPETGYKNDSKTCLQF
                     TKVNSQWANVYIKLSSAYRFDLTVQTKFKVLVYGNAGDKVLLKLENTDMGGNAWQTAT
                     HDLFYTIQESNEWEIAEFNFSGIGAGWDWTGTIFTSDVTTDPNFNTGFYNVVRIMVNP
                     GDATATYSVILDNWAGPTVNGWKK"
     gene            16017..18266
                     /gene="bglX"
                     /locus_tag="PK839_07270"
     CDS             16017..18266
                     /gene="bglX"
                     /locus_tag="PK839_07270"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007660647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HOG72571.1"
                     /translation="MLKRLLPILIAVALVGCKNQQLKQTEVGSGKFAVNKQVEAKVDS
                     LLSLMTLEEKIGQLNQLSGNGEVTGPISFPAEYMEALRKGLVGSMLNINGAAYTYKVQ
                     KVAVEESRLHIPLIFGYDVIHGYKTIFPIPLGEAASWDMEAIEKSARVAAIEASAAGQ
                     HWTFAPMVDIARDPRWGRVMEGAGEDPFFGSAVARARVKGFQGSKLSDTSSIAACAKH
                     FVAYGAAQAGRDYNTVDISERTLYEVYLPPFKSALDAGVVTFMSSFNELNGIPVTGNK
                     AMVNDLLKEKWGFNGFVDSDWGSIREMIYHGYAANEYEAGYLAMSSGVDMDLEGRIYL
                     AQLKKLVEDGRITVEQLDEAVRRILRVKFELGLFDNPYKGCDSIREKELLLNPRHLEI
                     AREVTRKSLVLLKNENVLPLSRNIRSIAVVGPLADNQDELIGTWSGRGEGKHVVSVLS
                     GIREKLPNGNIYYAKGCEIVGENKAGFDEAINAARKADAVVVVVGESAMMSGEALSRA
                     FLDLPGVQKDLVMELNALHKPLVVVVMNGRPLTIEWMDKAVPAILEAWLPGTMGGLAI
                     ADVLFGDYNPSGKLPMSFPRNVGQIPIFYYQKSTGRPPRQEERYTSKYIDSPVSPLYP
                     FGYGLSYTTFEYSNLSISSPSIKMDETLKVSVDVRNSGKYDGEEVVQLYVRDMVGSVT
                     RPIRELKGFKKIFIKSGESARVEFSLTSNDLAFYNQNMEYIAEPGDFMLFIGGNSDAS
                     LGLGFRLEE"
     gene            18394..20208
                     /locus_tag="PK839_07275"
     CDS             18394..20208
                     /locus_tag="PK839_07275"
                     /inference="COORDINATES: protein motif:HMM:NF012925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycosylhydrolase"
                     /protein_id="HOG72572.1"
                     /translation="MKKLLLIICALTITSSIFSQIQVMVWNDEFSYSGLPDATKWGYD
                     VGGSGWGNNELQYYTENRLENARVENGNLVVEARKENYGGMEYTSARLVTRQKGDWLY
                     GRMEAYAKLPSGRGTWPAIWMLPTNWAYGSWPKSGEIDIMEYVGYDPGVVHGSIHTEA
                     YNHVIGTQVTSTINVPTAETAYHLYAVEWSPEKIDIYVDNTKYFTFANEHTDYTTWPF
                     DKAFHLLLNIAVGGNWGGAQGVDPNIWPQKMLVDYVRVYQSLSAEQIFITGPGYVAPN
                     QTGVSFTIPNIVNASYIWTIPNDATIVSGQGTNSIVVNWGEQAGEVSVQITHPQAGGT
                     YTYNVKTSTTPSGDRYSLVNFKHDGIEGWSGMLTGPNTINLNLQDSLLRVDYNITDPS
                     AFPYFAYTLPNPVDMSSFTVLNIWMKTWNFSNSVVLRADLFDTDNRLTDVSPVFKFSP
                     VEPGGQFHVYSFDFKNKWGSNSPEYGQVVNQHQVKGVRFYVDYGIYGKVAADSLWLSD
                     IAISTHPLSASYIQQKSQFLSVYPNPCNDYICLYFDSQVVTTYQVSIYTIEGSLAGYF
                     ESSQNPCFISTKQLPSGVYVVTTLNSEGFSARVKLVKL"
     gene            complement(20231..>22213)
                     /locus_tag="PK839_07280"
     CDS             complement(20231..>22213)
                     /locus_tag="PK839_07280"
                     /inference="COORDINATES: protein motif:HMM:NF019136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triple tyrosine motif-containing protein"
                     /protein_id="HOG72573.1"
                     /translation="NLWLGLDNGIDYMELNSPISIIDEGLGLFGSCYSTILYNNKLFV
                     GTNQGLYVCDWPIRKNDIGGSFPFQRVEKTNGQVWCLEVIDGKLYCGHNFGTFEIDSK
                     TNVEHTSTEEGSWTFLKLKNHPNKMLVGKYKGIHLYVKENGSWRFLKKVKGFDESCRF
                     IAEDENENIWMAHGYKGVYRLVLNEEKDSVKYSYLFDNTKGFPVKTGINMEKLRNQII
                     FITQQGVFKYNALIDRMEPYSEFNRKLDDTINIRRLTEDSEGNIWILKSNDVKILKQQ
                     PDGKTSLEPTPLVLFGNSFVSSYENIYIPDISNVFLGTENGLIHYNPSYPTADSIKFK
                     CYIKSMQTLSRKGEKIFTDYKINRTQPISIKYSNNSIGLTFTSPTYNAGEIAYSYKLE
                     GYDTGWSEWSSNPIKEFTNLPYGNYTFRLKALDAKGRESDEVELKFTISNPWYLSLWA
                     FIAYGITCVLITLILRAAVKAKIRNATTKVKEQKNLEMKVKEEEHKREVLEAEQEIIR
                     LQNENLKVQVEHKNSELASIALQIAHKNEFLNQLKCKLEALTKNTNPALQKEVLELIN
                     KIDFDLRMDDEWKRFEFHFDEVHVGFLKRLKDLYPTLSPNELRLCAYLRLNMTTKDIA
                     QILNISIRGVEISRYRLRKKLGIEGDTNLIDFMINL"
BASE COUNT         6005 a         4602 c         5106 g         6500 t
ORIGIN      
        1 aggttagctt ccagtttttg gaaactagcc ttggcaacca tgggtattgg agttgctttt
       61 tttacaggta atcctctata ggcgcacagg tgcaaaccaa gtttctatct ccgtatcccg
      121 agtcaatttt acttacgtaa ggccaaaact tattggaagc aatccatgtc aaggggtatg
      181 cagctttttg acgggagtag ggatgatgcc actcgtcggc agacacctcg ggagcggtat
      241 gaggagcgtt tagcaccagg ttgtcggttg gggttgcatt tttacctgcc ttgatgtcct
      301 ccagctcagc aaggattgaa atcatagcct caataaacct gtcgagttct tgtttcgatt
      361 cgctttctgt tggttcaacc atcaagctct cgtgaacggg gaatgaaacg gtaggcgcat
      421 gaaatccata gtccataagc ctgcgggcta catcacttgt atcaaccccg tatgttgttt
      481 taaagtgatg gcagtcaagg atcatttcgt gggcaacccg acctgtttca ccggtgtaaa
      541 gaatcttaaa gtgctttttt agcttggctg ccatgtagtt ggcgttaaga atagccattt
      601 tggttacctg ggttaacccc tcggcgccaa gcatcaggat gtagccatag gagataggta
      661 ggatgctggc actaccccaa ggtgaagccg ctacagccga aataccgttt tctccaccgg
      721 tttttactac ggggtgggag ggaagaaatt tggttagatg tttggctaca gcaatagggc
      781 caacgccagg gccgccacca ccatgaggta tggcaaaagt tttatgaagg tttaggtggc
      841 aaacatcagc tccaatttct ccgggacttg ttagtccaac ctgggcattc atgttggctc
      901 catccatgta aaccaagcct ccattcttat ggattatatc taccatttca cggattttac
      961 tttcaaaaac accgtgagtt gaggggtagg ttaccatgaa agccgaaaga gtttctcggt
     1021 actgctcggc ttttgctttt agatcgtcca cattgatatt ccccatctca tcacaagcaa
     1081 ctaccactac ctgcatacct gccatagcgg cactagcagg gttggtgcca tgggctgatg
     1141 atgggattaa tactacattt cgcttgctat caccatggct taggtggtat tctctgataa
     1201 ccattagccc ggtgtactca ccagctgctc cggaattggg ttgaagtgat actgaatcaa
     1261 aacctgtgat ttcggcgagt aggtgctcca gctccgaaat gatttcgtgg tagccggcag
     1321 cctggtcggc tggtacaaat ggatgtatat tagccatttc cggccagctt aaaggaagca
     1381 tggttgctgc agggtttagc ttcatggtgc agcttcccag cgaaatcatg ctatggttaa
     1441 gcgcaatatc tctacgttca agttttttga tgtaacgcat catctcggtt tcggattggt
     1501 agctgttgaa taccttctcg gtcaggtagg gtgacctgcg ttgtaagttt gcaggtattc
     1561 cattagcttc ctcaacagat gaaatggggt tgtgctcttt tccaatagcc ttggcaaaca
     1621 cattcaacag ggcgttgatg tcgttaattg ttgttacctc atcaaagcta ataccaattg
     1681 atccgttagg cttatagttt agattcagct cattttccaa agcatttcga cggatatcct
     1741 ccaggctaac acctgctggt aaagtaactt cgagggtgtc aaaaatattt ttaatatttt
     1801 gtgtatagcc aagcagttgc agctgcttgg caacaagcat ggctacgcta tggatatatg
     1861 aggcaattct acgaattcca cttggaccat ggtaaactgc atacattccg gccatcgtag
     1921 ccagcagggc ttgtgcggtg caaatattgg aagtggcacg ttcccgttta atgtgttgtt
     1981 ccctggtttg gagagccatt cgaagcgctt tattcccgtg tcggtcaatc gaaacaccaa
     2041 taatccggcc gggcatgctg cgtttaaatt cgtccttgca ggcaaagtat gctgcatgag
     2101 gtccgccaaa ccccattggt attccgaagc gttgggttga gccagctaca atgtcagctc
     2161 cccattcgcc cggaggggta attaaggcca ggcttagcag gtcggccata acggtaacca
     2221 gggttccggc ctcgtgagct tttgctgtaa atgagctgta atcacggata ttccctttag
     2281 ccgaaggata ctgaatcaca actccaaact cacgtcctgt aaagctatat tcgctgtagc
     2341 agccggtaac tacatcaatt cccaacggtt ttgagcgcgt ggtaataacg gctagcgttt
     2401 gtggaaaaat gtcccgatcg acaaaaagta tatttttccc ggccttaaca gcctcccgtg
     2461 atcggaggtt atgcatcata atcatggcct ctgctgcggc agttgcctca tcaagaagag
     2521 aggcgttcgc aatctccatt cgggtgaggt cgataaccat agtttgaaag ttgagcaggg
     2581 cctctagcct accctggcta atttcagcct ggtagggagt gtaggacgtg taccatccgg
     2641 ggttttcaaa tacgtttcgt aaaataaccg aaggggttac agttccatag taccccatac
     2701 cgatatatga tttgtaaagc ttgtttttag ccgcaatttt cttcattctt cccagaaact
     2761 cgtattctga aacagggtta gggaggttta atggctttct cagcctgatg ttttgaggaa
     2821 tggtttgatt tataagttcg tctaacgttg aaacgccaat ttccttaagc atttcatcaa
     2881 tttcatggtc gcgagggcca ttatgacgtg caacaaaatt atcggttgtc attgtgttgt
     2941 gttttaatta gaggatgtat aaaagttgag caaaattata ttttttaagt acacaggcta
     3001 tgtgctgggt agggctttaa acatgttata aagcattact gaaaaattta agtcttgatg
     3061 aaagggttac ttctccgttc gtctccaatg gtagtgcttt cgccatggcc ggggtaaaca
     3121 gccacatcgt cggggaggac cataagctta gtggtaatgc tcctgataat ggcatcgtag
     3181 ctgccgccag gaaggtcggt acggccaatt gaccggtaaa acagagtatc accggttagt
     3241 agcattttct cgttttgaag gtaaaggcaa atactgccgg gtgtatggcc tggggtatga
     3301 ataatgccaa ttgaaccatc tccaagcaga agggtattgc catcggccac tattttatct
     3361 atttctgggg ttttatcaac cgaaaaacca aaggacatgg cctgttgcgg aaaggttttg
     3421 agtaagggta aatcatccga atgggcatag gcttcaacat tgtagatgct ttttaggagg
     3481 tttaagccca gcaggtgatc aacgtggcag tgggtattga caaaatattt tacctttaat
     3541 tcattgtcgt caacaaacct tttgattaaa gcaacctcgg tggcattata gcaaccagca
     3601 tcaacaatgg ctgcattgtg gttttcgtca gaaatcacgt atgtgttctc ctgaaagggg
     3661 ttgaagacaa aaacttttac tttcatcggc attggcaaat tatgttataa gtattcaaat
     3721 cattaacttt tttttgaatt tcttgcaacg agtggatgag tccaaaaggg gcatgctacc
     3781 agccagggaa aatgcttatg attggttggc tttagtttta ctacagtgca aaactcgttt
     3841 ggaagcgttt tcgatgtaaa caaaaacgtg tgggaattat agcggtgtgt tggtaaatgg
     3901 ttacatgtgt tggtaggcat agataggctt gttacttgct tttttccact ccttttaaca
     3961 acggaacaaa gataaagttt ccgtgggtag tctttcttac ttcggtgctg ctaatttttt
     4021 caactagcgt catcacctga ccgttttcat ccccaaccgg aataaccatt cttccaccaa
     4081 ccgccagctg gtcaacaagc tcatcaggaa tggttggcgc acctgcggta acaataattc
     4141 tgtcgaaagg gctatagccc ggtaatccct tatacccatc gccaaaatga aagctggggt
     4201 gatagcccat ttcctccagc agcgatttgg cgttaaggta tagctctctt tgcctttcga
     4261 tggtgaacag cctaacgccc agctccataa ggattgcagc ctggtaaccc gagcctgttc
     4321 ctacctccag caccttgttc atgtgttcaa cctgaaggag ctgggtttgg aacgccacgg
     4381 taaagggttg agatatggtt tgcccatatc caattgggaa agcctggtcg ctgtaggcaa
     4441 actttaggaa ggaactttcc ataaaaaaat gacggggaac ccggagcatt gccccaagca
     4501 cctgcttatc cgtaatcccc ttcgattcaa tttcttcaat taaccgtttc cgtagccctt
     4561 tatgcttata ggtgtcctgc tgcatcgcaa cttttatcaa gttaaaatgg tcgttcaaat
     4621 atacatctaa aagggcattt gaaaatttta aatgtaatag acagcaaaag agatatttac
     4681 actttttttg ctatacctaa tttttttatg ccagcttgtt gtatcatggt gttgctattt
     4741 atttgaacca ttgtagttga taagttaccc gactaaaatg ttggagatga cggtaaaatc
     4801 tataagtttg caactattca cccattcaac atatgcttaa acttggtttt tttactgata
     4861 atacgcagct ggctgaattg gtaggagatt tagggagaca agccaatata gaagtggagg
     4921 ggatatgggc agaacccctt ctaaatggtg acgacaaacg gaatttttcc aattgtttca
     4981 gtgatcctga agagctatat ggcagtattg atgtgggagt tatttccatt acatcatccg
     5041 agctctttat ggatatagtt acggcagcca ttaaaaatgg agtgcatcct ctggttgttg
     5101 gactgcccga ccttccgctt tctacgtata atcagctgag cctgcttagc gctgagatgg
     5161 gggtggagct tggttttagc catatctacc accggtttag cgggtttaat tatcccttag
     5221 tcagcccaat aattgcccat ctgacacgtg gaataaccat gaaccagctt aatcaatttg
     5281 aaatggcggc tacgcttagg ttcgatatag ccactattct atcgctaact acaagtgaag
     5341 taagaagaat tagaacttat gtgctgcccc aatcgggtga atgtccaagc tgcatgatgg
     5401 tttttatcga tttctttaac aactcagcaa ttacctataa cctgacgtta ggggctaaaa
     5461 gaaatttttt ggaacttcgg cttaactcaa ttggtgaggc aattgaactt tatgaggatg
     5521 agataaaact tgcaacaagc tcccttggcg ggatgatttc gactagcatt tttttagatg
     5581 atttaaacaa ctttacgggt tgtattggca tccaaaggga gccgttgctg ggcatttcat
     5641 ttgtaatgaa aatacttagt gcggttgagg cgatttctca aaaaatggcc aggtagcgtt
     5701 acttatttct ttctaaagaa atcttgatta acaaggctaa tttttagtgg tttgacaaag
     5761 aattacctta aaatctggta catactgttt gatgtcgttg gaagcgcagc tgcctggact
     5821 cttttctgta tttatcccgc aaaggatgtt gggttggagt ttttaagagc aggggcaaca
     5881 tttgagttcg atgggaagtt ctacgccagc cttgttttct tcgtactgtt ttggctagcg
     5941 ctgtttatag ttgcagggtt ctacaataat ttgtacagaa aatcaagact caaggaattt
     6001 acaaaatcgg taggctatac gtttgtgggt accgttgtga ttttctttat acttcttctt
     6061 gacgacagcc tggcaacata caatgacaac ttttccatta ttggctccat gttcattttg
     6121 gaattaacat ttacctattt cccaaggctt gtgcttacaa cgggaactat tcggaaaatt
     6181 cataggcgaa ttataggctt cccaacgata attgtaggta atgggtttaa ggcgctgcaa
     6241 gtttacaggg agcttgaagc cgaaaagaaa tctcaaggtt ttaggtttgt tggctacatt
     6301 gcccaggtta ataaccatga ggacatgctg caaagtcagc aaaaaaggtt gggggagatg
     6361 gattcaattg cagaagtagt gaaaaagcaa ggtgttgagg aggttatttt ggcggtggag
     6421 ggagaagatg tctcaacact tacccgttgt atcaacgagc ttttgcccct gaaagtgaat
     6481 ttaagggcta ttcccagcat gtacgacttt ttgcttggaa gggttaagat gaccgggatt
     6541 ctgaacgcac cattaatcag cgtttcgttt gacctaatgc cattatggca agccaacatt
     6601 aagcgtatag ctgattacac ggtatcggct ttagccatag tcctactttt gccggtttct
     6661 cttattcttg gaattgcgat aaggtgcaca tcccaagggc ctataatttt taagcaggag
     6721 agaattggac agtacggccg gcctttttgg atatacaagt tcaggagcat gtatgctgat
     6781 gccgaaaaat cagggccgac cctttcaggc aaggatgatc caagggttac acctattgga
     6841 cggtttatgc ggaaaactaa acttgatgaa attcccaact ttatcaatgt gttaaagggc
     6901 gaaatgtcgt tggttggccc acgtcccgaa aggcagtgtt atatagaaca gattgttcgc
     6961 atagcaccac actacgttca ccttcagaag gtaaaacccg gtattacctc atgggggcag
     7021 gtgaaattcg gctatgctaa aaatgttgat gaaatggtgg agaggctgaa gtacgatata
     7081 ctgtatcttg aaaacatgtc gctttttgtg gacctgaaaa ttatcattta caccatttta
     7141 acgattttta aagggaaagg actttaaccc ttaagcagca aaaagttctt aataggtttg
     7201 tgtatagctc cttttggcgt aagcaggctt tggtaaaagg ttacttcctc aatctccgat
     7261 tcctgaaagg tgtagttttg cagcttttcg gtcatatcat aaaacgctct aggatttctt
     7321 ataaagttaa cccgaccaag ggtaatgtgg ggcataaaaa ggcctttttc cctatcaaat
     7381 cctaagggga gaagcgcttt gtcaattgtt tgtttaagat tgttcagggt ttcattttgc
     7441 tcaatgccaa cccatataac ccggggtgta ggacgcccaa aacttccaag ctctttaact
     7501 ttaaggctga atgagttgaa gccagtgagc gatatagaaa gcactttaga aacgtcgcct
     7561 acaagatcaa ccggtatttc tcccagaaaa cggagtgtga ggtgaagcat gccctcgtca
     7621 acccagttaa tgaaatcgag gttatgctgc tgcttaagct tgtgccagca gtttaaaagt
     7681 ttggcttcag gcttaatgcc aaccgcaatg aaggttcgct cctttttcaa ctttcatgga
     7741 gatttatgta ctttccttgc aaaactttta aaaagttgta aattgtagcc aaaaataaaa
     7801 aaaattgact gggcatggcc aactcaaaaa aatttgctgt ggttctttcg gggtgcggcg
     7861 tactagacgg ctccgagatt cacgaagcag taatgacgct ttacgctatc actttatcgg
     7921 ggagcagcta tgaaattttt gcccctgatg taaaccagca tcatgtggtg aatcacctta
     7981 atggaagcga ggtggaccaa acccgtaacg ttcttgttga atcggccaga attgcacgtg
     8041 gaaagattaa accgcttggc gactttagct ccgatgtgtt tgatgctatt attttccccg
     8101 gagggtttgg ggtggccaag aatttatcgt cgtgggcttt tgatggagcg ggctgcagcg
     8161 tgaatgccga tgtggctaat gcggttcggt ctatgcatag gctgcataag ccaataggag
     8221 cgctatgtat agctccggtt gtaattgcca ggatacttgg aggagttaca gtaacaatag
     8281 gaaaggatgc tgcaacagta aaggagatag aaaccctggg tgcaagacat cagatggccc
     8341 tgcaaacgca ggttgtggtg gatgaaaaaa acatgatttt ttctacacca tgctacatgc
     8401 tcgatgcaac aattgtggat attgccgagg gggccaaggc tatagtttcc gaaatgctta
     8461 aatggatata gcgggagaat atattatagc aagccttttt cataaagacc tataagctcg
     8521 tttagcagct tatcctcacc ttcagggtca aatttaacct taaggtttga accaaaaagt
     8581 cgtgcaacac cctgccaaaa tgttgctgaa aatccaccct tcagctcttt tactaaatcg
     8641 taggaggttg cgccggtttc cctgtcgatg agcttcatca gcatttttcc ttgagaaacg
     8701 gtgaattttc tcaggtcctt ctcatactgc atccggagct ccttttcggc ctggttgatg
     8761 tattccctgc gttctctttc ggtcttaatc atttttagat cctcgttcat ctcaagtagc
     8821 ttgtacttgg caagctgggc gtaggggtag gtaagcttta aatttctaat cattttatag
     8881 tactggttca tttcgctacg gcttttaaag cgccgtttgc cgtactttac tacctctcgc
     8941 aacttgatgt agggtattgt atcaccctcg tagaaaacaa tagtataaag gttaaccaca
     9001 accgaatctc taacctgagc atgcgagtga cccgatgaca aggtgattcc cagcaggata
     9061 gccaatggta gtagcttact tttcgtcatg tttatccttg aacttctcat ttgcaaaagt
     9121 aacattaaaa caaccctttc taccgaatgt ttaccttatg cgaaacggca aaaatcaact
     9181 tttatgccat agttgagaat ttgtaaaaac aagcctgctc aaattataaa aattgcggcg
     9241 gcattaaacc aaattttaca gaatacattt aaattataat agcttaaaat acagcattat
     9301 aaatcttacc tcaggcatag agacgtacgg ttgacgtact gctccgaaaa cgttttcgac
     9361 agatgagaaa cttttgaaat tattatatag tggttatgat gtgggtggtt ttttgacatg
     9421 aaccagcagg atgtaatacc ttgcctcctg ttaaccaaaa accaaaccaa tgaaaaaaac
     9481 actactgact ttagttttga tcacttgggg gtgggttctg tcagcacaag aaattacagt
     9541 tacgggaact gtaatttcgg ctagtgatgg aataccacta ccgggtgttt cggttaccgt
     9601 taaggccaat ccttcaattg gaacagccac cggtattgat gggcgctaca gcatcaaggt
     9661 tgatgccaac gctacacttt tgttctcatt tgttggaatg gaaacaaaag aaatgcctgt
     9721 aaacgggcaa acaataatta atgtttccat ggttgaagct acccaggttc tcgacgagtt
     9781 ggttgtagtt gggtatggca tccaaaagaa gagcctgctc acctcttcgg tagccaaggt
     9841 gggaagtgat gaaattgcca agacaaatcc cttgcgagtt gaacaagctt tacagggaaa
     9901 aacggcaggg gttcaaatta tttccaactc cggccagcct ggggatggat taactgtacg
     9961 tattaggggt attggtacta atggtacgtc agaccctatt tacctggttg atggaatgcc
    10021 agtgggtggg atagactacc ttaatccaaa cgatattgaa tctgttcagg tgttgaagga
    10081 tgcttcggct acggcaattt acggtgcacg aggtgctaat ggagtagtgc ttataacaac
    10141 aaagagcggt tcaaagggaa aaatggccgt tacctacgac tattctttcg gtttacagaa
    10201 tgcatggcgt aaagtttcgt tacttgacgc cagcgagtac gccttgatta tgaacgaaag
    10261 ctatgccaac gataatgctg caatcccttt tgctgatgta gcatcactga ttgatacggt
    10321 tggtagtgga accgactggc tcgatgagct attctataaa aacgctcccg tgcagaacca
    10381 ccagttctcg gtaagcggtg gcaacgacaa ttcttcgttc tactcatcct tttcctatac
    10441 tacccaggat ggtattgttg cgaaaggaaa gtcgaactat gaacgttata cctaccggat
    10501 taactccaac catcagtatg gaaagtttgg atttggcaac aacctttcat acagcaataa
    10561 gaaaaacaga gggattgatc ctaacgccga atttggcggg ccactggcca aagcagttaa
    10621 tatggacccg cttactccgg taaaatatgc cgatggcaca tggggagtgt ccagctatgc
    10681 cgcccaggaa gttgtaaacc cggtggcttt tctaagcgtt cttcacagcg attataccga
    10741 gaacaagctg gtaggaaatg tgtgggggga gctggagcta gtaaaaggct taaaggtgaa
    10801 gtcaaatttt agtattgact atgccaacgg gaatagccgc acgttcaccc ctgtttacga
    10861 tctgggagga aacattaaga cgctggtttc ggaggctagc gtaaccgaga acaggtggtt
    10921 tacctggcag aacgagaata cgctgtcata tagcaaccaa atagacctgc atagctttac
    10981 cgccatggtt gggatgacag caaataagta tcatcatgaa tttgtaggtg gtagaaaaac
    11041 cgacctgcta tttgatgatt ttgaacatgc ctggataaac aacggcaccg atgaggaaag
    11101 ctacagggcc tggggtggtg ccgatgagca tgccttgctt tcctactttg cccgtgttaa
    11161 ctataacttt aacgagaagt acctcttgga ggctgtttta cgagccgatg ggtcatccaa
    11221 ttttggatcg aacaaccggt ttggttactt ccctgcattt tcagccggct ggatattatc
    11281 caaggaagac ttccttcagg gatttactcc cttgtcattc ctaaaggtta gagctggttg
    11341 gggtcgaaat gggaatgaag ctattggagc attcaagtac acctcaataa ttggttcagg
    11401 aagcaagtac acatttggtt cagatgaggt aataacggta ggttccaatc cttcgggcat
    11461 acccaatccc gatttaaaat gggaaacctc cgagcagtac aacataggta tcgactcgcg
    11521 gttttttaac gagaggttaa actttactct agacttgtat aacaagaaaa caaaagactt
    11581 actcgtagtt gctcccattc cagggtatat tggtaatgca gcaccaaccg taaatggtgg
    11641 aacagttacc aacaagggtg ttgagctgga gctgggttat aaaaccagca tcaacaagct
    11701 ggatgtagat gtaagcctta gcggcgccta caataaaaat gaggtaaccg atataaataa
    11761 tgccgagggt aaaatttatg gagcaggact tgccgttgga atgagtaata tctgcatgat
    11821 gcaggaggga tttccaatag cttacttttg gggttataaa actgccgggg tctttcagaa
    11881 tgaagctcaa attcttagct acatttcatc cgatagcacc gttattcaac ctaatgctaa
    11941 acctggcgac cttatttggg ttgataaaaa tgatgacggt aaaattgacg ataatgaccg
    12001 tatgttgatt ggaaatccat atcccgattt taccgccggg ttaaatgtta acctggcata
    12061 ccggaacttc gattttaaca tgttttggta cggtgctttt ggccaggata tattcgatgg
    12121 aacccgccgg tacgatctgc ccatgtcaaa ctggaatgct agtgttctcg accgttggac
    12181 aggcgaagga acctctaatt caaatccaag ggttaccatt gccgatccca acaaaaacta
    12241 tgtaaatgtt tccgactttt atgttcataa tggctcctac cttcgactta agaaccttac
    12301 cctgggctat actataccta ctactattag ccaaaaggtg aaaattcagc ggatgagagt
    12361 ttttatgtcg gtttccaacc ttttaacctt tactagctat aaaggttttg accctgaaat
    12421 tggtgcaaaa tctgcacttg atgttggtat tgaccgcaac atttaccctc aatcacggac
    12481 cttcatgttc ggagtcaacc tgaatttcta aaccacaaaa ttttatcacc atgacaaaaa
    12541 ttaaatactt tgctgttacc ctagtgttac ttctcggaat gggttcgtgc tctcaggact
    12601 ttttggagat ggagccttta accgacaggg ttgagggtaa cttttacaaa acagagaagg
    12661 atgcctttga ggcattggtt tctatatatg atgttctcca gtggcagtcg agctacggtt
    12721 atcatccttg ggatttagtt tctaacgttc tttccgatga tgcttttagt ggtggatctg
    12781 gccctggcga tagaccaggt cttaatagaa tgggaaagtt ttctagctat accaccgatg
    12841 aggaggcgct tggtttatgg aaagacaggt actccggtat atacagagcc aatatttttc
    12901 tcgaaaaaat tgatggaatc caattcaaag atgaagcact taaagatcgc tacattgccg
    12961 aagcacgttt tcttagggga tacttttact ttgagctggt tcagttctac ggtcatgttc
    13021 ccttaatcct taaaaccctt accacatctg agtataagca atctcaggcc aatcctgctg
    13081 atgtgtacaa tcaaattgca agcgatctgc tctttgcatg ggaaaaattg ccaggcaaca
    13141 tttcagctgc tgagtatggt agggtttcaa aatgggctgc cggagcaatg ctcgctcgag
    13201 tttacctctt ccacaaggga tatggaaaag gtgttcttgg gattacttct gatttaactg
    13261 ttgacaatgt ccccataacc gaatcgaata ttgagcagat tgttgatgac ataattaatc
    13321 taagcggcca tgcccttgtt cctaactttg ccgatttgtg gggagtaaat catgaaaata
    13381 acaaggagtc catattcgaa attcaacaca ccgataaaag caactggggt gattggggct
    13441 ggagaaatgg aactgaaggt aactggaccg ttgtaatgtc gggatttcgt gatgtatccg
    13501 accctattta cgatttgggt tggggctttc agcctgcctc ccagtctctg gctaatgaat
    13561 ttgaacctgg cgatccacgc ttctccgtta gcattttgga tgcagctgct gaaaatctcg
    13621 tttataagcc tcagaattgc taccagcata ccggctacgc ctttaaaaag tactatccaa
    13681 agaaagccga taaaccgttg tcgaatcccg acttaaactg gggctacaac agggtggtaa
    13741 ttcgccttgc cgacgttctg ctaatgggag ctgagctata cctggataac gatcttctta
    13801 aggcacaggg atactacgaa caggttcgga agcgtgcttt tggccagtcg catgttgccc
    13861 caactttaac caacgaccag aacggccttg accttatcta ccatgaacgt agggtagagt
    13921 ttgccggcga aggcattaga tactgggatt tactacgccg tggtttaaac tatgcacagc
    13981 aaaaaattga tgctgcaagc agtgcaagcc cctacgacga aacatttaaa cctgcaacca
    14041 agggattact gcccattcca cagtcggaaa tctccatttc gggtaatatc ctggtgcaga
    14101 atgaggggta catttaattt attaacaata atctaatagc tatgtcgaaa aaaaatatac
    14161 tattcggagt tctggttcta gttggcctta cgtggcatgc ctgcgaacca tctgaagttg
    14221 gtaaacctga tgttggcaaa gcacctaggg ttgaagacct gagctttacc attacccccg
    14281 gcgaagatgc atttcacttt gtttttacca acacctcttc gttgaaggga attgcaaact
    14341 gggatttggg aaatggttct aaggctatcg ggaatactgt tactgcctat ttccctattg
    14401 ccaaaaccta cacagttacg cttaccctct acgcttcggg gggaagcgcc tcgctttcgc
    14461 aggagttgac tcaaaatgag acagactggg acttccttgc tagcccgctg attactgcta
    14521 taacaggggg cgttgagtct gccaacggca aaacatgggt tattgatagc attaatcccg
    14581 gacatttagg tgttgggcca gctaatgaaa ccaagcctag ctggtggtcg gctcaaccac
    14641 gagagaaaac aggacacttc ctctacgatg atgagtttac atttaagttg gttgattttg
    14701 tttacggtat cgacactcat ggtaaaaccc atgtaaacca cgatgggaat gctgatgagg
    14761 atggatttgc ggggggatac tacacctcat tgctttggaa tgatgcctat gatgctgacg
    14821 ttgctactaa tgatgcagca cgcggtgctc ttacctggtc aataaatgag gtaaacggaa
    14881 agtcttatat taacatttcc agccaaagcg gtaacatttc gtatgatgat ggaaatccca
    14941 gatcgtatga aattttagag tggaatgaca acttcttata ccttaggagt gcaagtgccg
    15001 gaaacgcacg ctaccacaaa cttattccta aaggttatgt taaacccaaa atatcctacg
    15061 agctgagcat tactcccacg ttaaatccaa atgagtatag catgcagctt tctaatgtta
    15121 ccattcccgc tgacctaacc atttctaaag ttgaatggga ttttggcgat gcttcctcac
    15181 ccaacgtgta cacttcaact gactataacg aggttgtaac ctttacctac atggctgccg
    15241 gcacctatac ggttaaggta accgttaccg acaataacca tgacacctat gtggttacca
    15301 gtcaggttgt tgttgctgct gatcaccccg actatgtgcc ctatattgag acgggcatgt
    15361 ccatctatgc caattttgac gatatattct gcaagtttgc tgttgataat gccgacaatg
    15421 tgggttcttt cagcttgatt gctaaccccg aaacaggtta caaaaacgac agtaagacct
    15481 gccttcaatt taccaaggtc aacagccaat gggcaaatgt ttacattaag ctttccagcg
    15541 catacaggtt cgatttaacc gttcaaacca agttcaaagt gctggtttac ggtaatgctg
    15601 gcgacaaggt gttgcttaag ctcgagaaca ctgatatggg aggcaatgca tggcaaactg
    15661 ccactcatga tttgttttac accattcagg aatctaacga gtgggaaatt gccgaattta
    15721 acttttcggg cattggcgcc ggatgggatt ggacaggcac aatctttaca agtgatgtta
    15781 ctacggatcc taactttaat acgggcttct acaacgttgt tcgcattatg gttaacccgg
    15841 gtgatgctac tgcaacctat agcgttattc tggataactg ggcaggccca acagtaaacg
    15901 ggtggaaaaa gtaaattttt gaaatgcacc agaggtaatt cctttgttgg aattatctct
    15961 ggtttgctcc gacaacgcat tccacttcaa tatttttata cactaaaatg aaaaagatgc
    16021 ttaaaagatt attgccaatt cttattgccg ttgcccttgt aggatgcaaa aatcagcagc
    16081 tgaaacagac agaagttggt agtggcaagt ttgccgttaa taagcaggtt gaggctaagg
    16141 ttgactccct gcttagcctt atgacgcttg aggagaaaat cggacagcta aatcagctat
    16201 ctggaaacgg cgaggttact ggcccaattt ctttccctgc cgaatacatg gaagcactcc
    16261 gaaagggact ggttggttcg atgttgaata tcaacggtgc agcatatacc tacaaggtgc
    16321 aaaaggttgc tgttgaggag agccggttac atattccttt gatttttgga tacgacgtta
    16381 tccacggata caaaacaatt ttcccaattc cgttgggcga ggctgcatca tgggatatgg
    16441 aagctattga aaagtcggcc agagtagctg caattgaagc gtcggcggca gggcagcatt
    16501 ggacatttgc accaatggtt gatattgccc gtgatcctcg ttggggtagg gtaatggaag
    16561 gtgctggcga agaccccttc ttcggttctg ccgttgctag agcccgggtt aagggatttc
    16621 agggtagtaa gctatccgac accagctcta ttgctgcctg tgccaaacat tttgttgcct
    16681 atggcgctgc ccaggctggg agggattaca acacggtaga tatttccgag cgaacactct
    16741 acgaggttta cctgcctcct tttaaatcag cgcttgatgc aggtgttgtc accttcatgt
    16801 cgtcattcaa cgagcttaat ggcattcctg ttacaggaaa taaggccatg gtaaacgatt
    16861 tgcttaagga aaaatggggc tttaatggct ttgttgattc tgattggggc tctattcgtg
    16921 agatgattta ccacgggtat gctgccaatg agtatgaagc cggatacctg gccatgagct
    16981 caggagtgga tatggatttg gaaggtcgta tctatttagc ccagcttaaa aagttggttg
    17041 aggatggtag aataaccgtt gaacagcttg acgaagctgt tcgcagaatc cttagagtga
    17101 aatttgagct aggtttgttc gataatccct ataaaggttg cgatagcatt cgcgaaaaag
    17161 agcttttact aaaccccagg catttggaga ttgctcgcga ggtaacccgt aaatcgctcg
    17221 tgttgcttaa gaatgagaac gttcttccgt taagtaggaa tattcgctct atagccgtag
    17281 ttggccctct agccgacaat caggacgagc taattggaac atggtcaggt agaggtgagg
    17341 gcaaacatgt tgtttctgtg ctatcgggaa ttcgtgaaaa gctgccaaat ggaaatattt
    17401 actacgctaa gggctgcgaa attgttggtg aaaataaggc tggatttgat gaggccatta
    17461 atgctgcccg aaaggctgat gcggtagtgg ttgtggtagg tgaatcggcc atgatgtctg
    17521 gtgaagcgct tagccgagct tttctcgatt tgccaggagt gcagaaggat ttggtaatgg
    17581 agcttaatgc tctccataaa cccttggtag ttgtggtaat gaatggtcgc ccgctaacca
    17641 tagaatggat ggataaggct gttcctgcaa tactcgaagc ttggcttccc ggcactatgg
    17701 gaggtttagc cattgccgat gtactttttg gtgattacaa cccttcgggc aagttgccca
    17761 tgtcgtttcc aagaaatgta gggcaaatcc ccatatttta ctatcaaaaa agcaccggca
    17821 gaccccctcg tcaggaagaa agatacactt caaagtatat cgacagccct gtatcaccgc
    17881 tttacccttt tggatatggt cttagctata ccacctttga atatagcaac ctgagcatca
    17941 gctccccgtc tattaagatg gatgaaacgc ttaaagtttc cgttgacgtt cgtaattcag
    18001 ggaagtatga tggtgaggag gtggttcagc tctatgttcg cgatatggtg ggcagcgtta
    18061 ctcgacccat tagggagttg aaaggtttta aaaagatatt cattaaatcc ggtgagtcgg
    18121 ccagggtgga gtttagtctc acctccaacg atctggcgtt ctacaaccaa aacatggaat
    18181 atattgcaga acctggtgat ttcatgcttt tcatcggtgg aaactccgat gcgtctttag
    18241 ggttaggttt tcgattagag gagtaatttt gcatcaaacc ttcggatttg ttttgcgatc
    18301 cgaaggtttt tttgctttaa ctttcccaat cgatggctat attttaagag acgaaatatt
    18361 aaattcaagc tgactgttta ttaagaaaaa gtgatgaaga agctattact gataatatgt
    18421 gctttaacca taacaagttc tatcttttcc caaattcaag ttatggtttg gaatgatgag
    18481 ttcagctact ctggccttcc ggatgcaacc aagtggggct acgatgttgg cggaagcggt
    18541 tggggaaaca acgaactgca gtactacacc gaaaatcgcc tggaaaatgc aagggttgaa
    18601 aacgggaatt tagtagttga ggcacgtaag gaaaactatg gtgggatgga gtacacatct
    18661 gcccgtctgg ttacacgcca aaaaggcgat tggctatatg gtagaatgga ggcctacgcc
    18721 aagctacctt ctgggagagg tacatggcct gcaatttgga tgcttcctac caattgggct
    18781 tacggttcat ggcccaaaag cggtgaaatt gatatcatgg aatatgttgg gtacgatcct
    18841 ggtgtagttc atggtagcat ccacaccgaa gcttacaacc atgtcattgg aactcaagtt
    18901 acctcaacca tcaatgtgcc tacagccgaa accgcctatc acctttatgc tgtagaatgg
    18961 agccccgaga aaatagatat ctatgttgac aataccaagt actttacttt tgccaatgag
    19021 cataccgatt acactacatg gccattcgat aaagcttttc atttgctgtt gaatattgct
    19081 gttggtggaa actggggcgg agctcaaggg gttgacccaa acatttggcc tcaaaagatg
    19141 cttgttgact acgttagggt ttaccagagc ctttcggctg agcaaatatt tattacgggg
    19201 cctggctatg ttgctcctaa ccaaacaggg gtgtccttta ccattccaaa tattgtaaat
    19261 gcctcataca tatggacgat tcccaacgat gctactattg tttccgggca gggaacaaac
    19321 agcattgtgg tgaactgggg tgagcaagcg ggtgaggttt ccgttcaaat tacccatcct
    19381 caggcaggag gaacatatac ttacaacgtc aaaacttcaa ctactcctag tggtgacagg
    19441 tatagccttg ttaacttcaa acatgatgga atagagggct ggtcaggaat gctgaccggc
    19501 cccaacacca tcaacctgaa tcttcaggat tcgctactta gggtagatta taacattacc
    19561 gatccctcgg cttttcctta tttcgcctac accttaccaa accctgttga catgagctcc
    19621 ttcaccgttc tgaatatttg gatgaaaacg tggaacttta gcaactcggt tgtgctaaga
    19681 gccgatttat ttgatacgga taacaggcta accgatgttt cgcctgtctt taagttttct
    19741 ccggttgagc ctggtggcca atttcatgtt tactcctttg attttaaaaa caagtggggg
    19801 tccaactcac cggaatacgg tcaggtagtt aaccagcacc aggttaaagg cgtgaggttt
    19861 tacgttgact atggcattta tggcaaggtg gcagctgatt ccctatggct aagcgatata
    19921 gctatcagca cccatcctct ttcagcaagc tatattcagc agaagtctca atttttaagc
    19981 gtttatccta acccatgcaa tgattacatt tgtctttatt ttgatagtca ggtagttaca
    20041 acctaccaag tcagcattta cactattgag ggatctttgg caggctattt tgaatcaagt
    20101 cagaacccat gctttatttc cacaaagcaa cttccttcag gggtttatgt tgttaccact
    20161 ctcaatagtg aaggtttttc agcaagagtt aaactcgtga agctatagta ctcgctgaat
    20221 cctcttttcc ctaaaggttt atcataaagt ctatcaggtt agtatcgcct tcgatgccca
    20281 actttttgcg caaccggtag cggctaattt ccactcctct tatgctgatg ttcagtattt
    20341 gggcaatgtc cttagtagtc atgtttagcc tgagataggc gcaaagccta agttcgttgg
    20401 gtgaaagggt agggtatagg tctttcaggc gtttcaaaaa tccaacatgg acttcatcga
    20461 agtgaaattc aaagcgcttc cactcgtcat ccatccttaa gtcaaaatca atcttattta
    20521 tcagctccag cacttccttt tgtaaggccg ggtttgtatt ttttgtaaga gcctccagct
    20581 tgcattttag ctgatttaaa aattcgtttt tgtgtgcaat ttggagcgca atgctagcca
    20641 actcgctgtt tttatgttca acctgaacct ttaaattttc attttgtagc cttattattt
    20701 cctgctctgc ttccagaacc tcccttttat gctcctcctc cttaactttc atttcaaggt
    20761 ttttttgttc cttaacctta gtggtagcat ttctaatttt agccttaact gctgctctaa
    20821 gtatgagggt tatcagaacg caggttattc cgtatgctat aaatgcccaa agcgaaaggt
    20881 accatgggtt gctgatggta aacttaagct caacttcatc gctctctcta cccttggcat
    20941 ccaatgcttt tagcctaaag gtgtagtttc cgtaaggtag gttggtaaat tcttttatgg
    21001 gattgctgct ccactcggac catccggtgt cgtaaccttc gagcttgtag ctatatgcaa
    21061 tttcacctgc gttgtatgtg ggcgaggtaa acgtaagccc aatggagttg ttggagtatt
    21121 ttatgctgat aggttgcgtc ctgttaattt tatagtccgt aaagatcttt tcacccttgc
    21181 gggaaagagt ttgcatgctt ttgatgtagc atttaaattt aatgctgtcg gcagtggggt
    21241 agcttgggtt atagtgaatt aatccatttt cagttccaag gaaaacattt gaaatatcag
    21301 gaatgtaaat attttcgtaa gagctaacaa aggaattgcc aaaaagtaca agaggggtag
    21361 gctcgaggga tgtcttgcca tccggttgct gttttaggat ttttacatcg tttgatttta
    21421 gtatccatat attgccctcg ctatcctcgg ttaatcgtct tatgttaatt gtatcgtcca
    21481 gtttccggtt aaattcggaa tatggctcca tcctgtctat tagtgcgtta tacttgaata
    21541 ctccctgttg ggtaatgaag attatttggt ttcgaagctt ttccatgtta atccctgttt
    21601 taaccggaaa ccctttggtg ttatcgaaaa ggtaggagta ttttacgcta tccttttctt
    21661 cattcagcac aagccgatac acacccttgt aaccatgagc catccatata ttttcatttt
    21721 catcctcagc aataaaacgg catgactcat caaaaccttt aaccttttta agaaatcgcc
    21781 acgaaccatt ctcctttaca tatagatgta ttcccttgta ctttccaacc agcattttat
    21841 tcggatgatt ttttagcttc aggaaggtcc acgacccctc ttcggtggaa gtatgctcta
    21901 catttgtttt ggaatcaatt tcgaaagtcc caaagttatg gccacagtat agtttcccat
    21961 caatcacctc aagacaccac acttgtccgt tagttttctc tactctttga aaaggaaaac
    22021 ttccacctat atcatttttc ctaattggcc agtcgcaaac atagagcccc tggttggttc
    22081 ctacaaacag cttattatta tacagtattg ttgagtagca agaaccaaac aaccccaaac
    22141 cttcgtcaat aatagatatg ggcgagttca gctccatgta gtcaatgcca ttatctagtc
    22201 ctagccataa gtt
//
LOCUS       DAOSHM010000040        21673 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_1954_length_21673_cov_20.121982, whole genome shotgun
            sequence.
ACCESSION   DAOSHM010000040 DAOSHM010000000
VERSION     DAOSHM010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 21673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21673
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1954_length_21673_cov_20.121982"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..807
                     /locus_tag="PK839_07285"
     CDS             <1..807
                     /locus_tag="PK839_07285"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HOG72574.1"
                     /translation="MPGYDDNSSLRPNIYLQFGLSDFPNHSVSGLIFSNDLPVAIPGA
                     NISMQGFLTYNATTNEDGRFSIDSLLVDSYVLNVSGKGFVSYSDTLTVDGDMMLDTIK
                     LSSASYPVTFSVYASGEAVEGAEIAIEGYPVIYTNSLGVALTSLKDGDYSYNVTKTGY
                     NPILGNTFTVSGGDKNVDILLFPLSAGDSKLNAAAAYPNPFDSYLRIGGAAEVKYIKL
                     TSLTGQVVMEFRGNGSETYTLNTANLPAGFYLITMESASGEKLVKKVIKQ"
     gene            complement(936..1967)
                     /locus_tag="PK839_07290"
     CDS             complement(936..1967)
                     /locus_tag="PK839_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HOG72575.1"
                     /translation="MEEQYLNYTPQDFKSDQEVRWCPGCGDHAILSAVQKALPEVSKE
                     LKYNIERYVFVSGIGCSSRFPYYVNTYGFHGIHGRASAIATGVKVANPTLSVWQITGD
                     GDALAIGGNHFIHAIRRNIDVNILLFNNQIYGLTKGQYSPTSPLGKITKTSPFGTVEH
                     PFRPGELVIGAQGKFFARTLDVDVKLTSEIMVEAAKHDGTSVIEILQNCVIFNDGAYD
                     FISSKETRDDRVIVLHHGEKMVFGKNRDKGLVLSGLSLKVVTIGQGGFTMDDILVHDA
                     YNPNPGVHMMLANMEFPEYPIALGVIRNVKDSTYDDNVRDQVLEVMKDAKIKCVDDLL
                     NSGETWEVK"
     gene            complement(1970..3823)
                     /locus_tag="PK839_07295"
     CDS             complement(1970..3823)
                     /locus_tag="PK839_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014774311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HOG72576.1"
                     /translation="MEKQTGVQELDEVAVRFSGDSGDGMQLTGTLFADASALFGNDVS
                     TFPDYPAEIRAPQGTIGGVSGFQVHIGSKKVNTPGDYCDVLVAMNPAALKANIKWVKK
                     GGMVIADADQFNEKGYQRAGYKTLDAVTELNLQDYKVIMAPITTLTKESLKDSGLDNK
                     SIVRSKNMFALGMVYYIFDRPMNFTETYFETKFKKKPEIIEANKKVLQDGYNYAANIQ
                     AIPNTYSIKPSKILPKGKYRTVSGNTATAWGLIAAAEKAGLPLFCGSYPITPATDILA
                     ELSKRKDLGVKTLQAEDEIAGICTAIGASFAGNFAVTSTSGPGLSLKSEALGLAVMTE
                     LPLVVVDVQRGGPSTGLPTKTEQADLMQALWGRNGEAPVVVISATSPSNCFDAAFNAG
                     KIAMEHMTPVILLTDGFIANGSEPWLIPNMKEYPKINPPLVEEGTQGYMPYARDDKRL
                     ARGWALPGKKGLTHRVGGLEKDFLKGSLSHDPINHQRMVDIRAAKVERVREFIPEQKV
                     IGDSEGDLLVIGWGGTYGHLLDAVTDLRGKGKSVSLCHFSYINPLPKNTRDILSRYKK
                     LVVCELNMGQFANYLRMNFQEFNYLQFNKVQGLPFTVVELVEKFNQLLEGK"
     gene            complement(4020..4778)
                     /locus_tag="PK839_07300"
     CDS             complement(4020..4778)
                     /locus_tag="PK839_07300"
                     /inference="COORDINATES: protein motif:HMM:NF014037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mut7-C RNAse domain-containing protein"
                     /protein_id="HOG72577.1"
                     /translation="MVKKFSKHIELRFYGGLNHFLPASKRQVPFNLPYMGTPTVKDLI
                     ESVGVPHVEVFLIVADSISVGFDYKPEPSSQISCYPRFYNLSIDYGILRPPEPAPPVF
                     ILDVHLGRLASYLRLYGFDTLYSNCYSDDQILRLAAAENRIILTRDKGILRSGAAIHG
                     YYVRSTQPKEQLNEVVDYFSMACFADPFSRCSLCNGLVETVDRENVKGLVAENSYNHY
                     HSFYRCSRCGQIYWEGSQYKGILNLFGRHGPHHE"
     gene            4913..5431
                     /locus_tag="PK839_07305"
     CDS             4913..5431
                     /locus_tag="PK839_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HOG72578.1"
                     /translation="MAIIKPLRGFTPKIGENCFIAETAVIIGDVEIGDDCSIWYGVVA
                     RGDVNSIRIGNRVNIQDNAVLHTTYQKTTIRLGNDVSVGHGAIVHGAEVRDGALLGMG
                     CTVMDNAVIGEGAIVAANALVLENTTVEPGTIYGGVPAKFIKKVDAEVSREMNERIAR
                     NYLFYAGWYKQG"
     gene            5588..7642
                     /gene="metG"
                     /locus_tag="PK839_07310"
     CDS             5588..7642
                     /gene="metG"
                     /locus_tag="PK839_07310"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HOG72579.1"
                     /translation="MYKPFHPMSNYKRYLITSALPYANGPVHIGHLAGVYIPSDIYTR
                     YLRAKGADVISVCGSDEHGVPITIKARHENTTPQAVVDKYHAIIKESFKQFGIDFDIY
                     SRTTSPVHYETASEFFKKLYEKGVFVEKVTDQYYDEEVGQFLADRYITGTCPHCGNPN
                     AYGDQCEKCGTSLNATDLIDPRSTLSGSKPILKATRHWFLPLDKFQPELEKWILEDHK
                     EWKANVYGQCKSWLDQGLQPRAVSRDLDWGVPVPLDGAEGKVLYVWFDAPIGYISATK
                     ELTPNWEKYWKDSDTRLIHFIGKDNIVFHCIIFPSMLKAYGDYILPDNVPANEFLNLE
                     GDKISTSRNWAVWLNEYLVEFPGKQDVLRYVLTANMPETKDNDFTWKDFQTRNNSELV
                     AILGNFVNRAMVLTHKYFDGKLPARNTLTDYDNSVLHELPRIKERLEHNLEEFKFREA
                     LKEAMNIARLGNKYLADTEPWKLMEKDPRQVANILNIAMQITANLSIILEPFLPFTAE
                     KIRKMINFQSKEWKLAGLDSLLAPGHILDKPQLLFDKIEDDVIAVQLNKLQATKLVNQ
                     NSVKNVPEQKGSISYDDFSKMDIRVATVLDAERVPKTQKLLKLKVDTGIDVRTLVSGI
                     AEYFTPEEMVGRQVLVLANLEPRKIRGIDSHGMILMAEDTSGKLQLVSPAAKVSNGST
                     VK"
     gene            complement(7725..8837)
                     /locus_tag="PK839_07315"
     CDS             complement(7725..8837)
                     /locus_tag="PK839_07315"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOG72580.1"
                     /translation="MELEQVIQLLLSQFDFVSLPGLGSFVKRRNPAKVSDDRSSIEPP
                     SEYYIFDTSRTFDDEAITNYLCSTLGIDHKEAELRVTQLVERVNNALSSRKPFEFPGV
                     GTLLKDDNNNITLEFDASAAATESYGLSAIEVLPKQKADSKLTSPKENKKAAVAETQA
                     SSPLSTEQTTKIPTPKPAVAKKSRVRLWVPITACVVVVAVAVCWFTPALHFWEQSQTS
                     VSPSTTDSTSSMASVGNLPLASAIPDSIVEEEDSQVPDSISASGQPNATDEGGAEKIL
                     EQTTSKKVALSYTEPPVVETGKSFYIIAGSFTSEVNAQKFLSELAEKGYKPTLIPRNK
                     LYSVSLYTFSNRDRALRELERLKSEQTFEKLWLLSQ"
     gene            complement(9061..10050)
                     /gene="trpS"
                     /locus_tag="PK839_07320"
     CDS             complement(9061..10050)
                     /gene="trpS"
                     /locus_tag="PK839_07320"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOG72581.1"
                     /translation="MEIVLSGIRSTGKLHLGNYYGALLNFVQMQHEHKCFFFIADYHS
                     LTTHPTPTDLHGSVKQVLAEYLACGLDPEVATLYVQSDLPQVPELYLLLNMNAYVGEL
                     ERCTSFKEKIRKHPDNINAGLLTYPTLMAADILIHKATMVPVGKDQEQHLEMTRTFAN
                     RFNRLYKVDYFPEPVAYNFGKELVKIPGLDGSGKMGKSEGNAIYLSDDPKTIRSKVMK
                     AVTDSGPTQPNQEKSEPVKNLFTLMRVVSDPETYSYFEEQYSSCHIRYGDMKKQLAED
                     IIRITTPIRERIEEILANDAYLSQVAHHGAEKARESASKTINEVRQIIGFRNF"
     gene            complement(10295..13294)
                     /gene="secDF"
                     /locus_tag="PK839_07325"
     CDS             complement(10295..13294)
                     /gene="secDF"
                     /locus_tag="PK839_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecDF"
                     /protein_id="HOG72582.1"
                     /translation="MQNKGAIRFIAIALILVCIYQLSFTLVTRRVEKKAREYAMVGNE
                     IDRAKETHYMDSISSEPVYNFLYIRKYTYKECKEREINLGLDLKGGMNVMLEVSVTDL
                     IRSLSNNSQDSSFLKAMEVAQKMPGSEHFVTKFGKAFQQVAPNARLAAIFSTPELRDK
                     INFESTNDQVIKILRAEADNAINNSFNILRNRIDKFGVAQPNIQRLENSGLILIELPG
                     IKEPERVRKLLQGTANLEFWETYEFSELFNNFIAANNALAEIQDATAKTDSIKSTSNK
                     ELTAKKDTVTDELLAKLKATSTEDSLSATQADQTKKYPLFSLLQPNLYQDQPAPGPLV
                     GYAHVKDTARVNEILRMPKIKALFPVDVKFLWGVKPPKWDKSESLYELIALKVTSRDG
                     KAPLDGGVITDAREAFGETKGSAEVDMSMNAEGARVWARMTGENIGRCIAIVLDNYVY
                     SYPRVQSEIKGGRSQITGDFTINEAKDLANILKSGKMPAPARIVQEELVGPSLGTESI
                     NSGMNSFILSFILVLLFMMVWYSFKAGLVADIALFFNLFFIMGIMASFGATLTLSGIA
                     GIVLTLGMAVDTNVLIFERIREELRSGKGTIPSVNEGYRNALSAIIDGNVTTLLTGII
                     LYVFGTGPIRGFATTLIIGIITSLFTGILISRLIQTWMINHNINIRYSVKFTEHAFRN
                     LHIDFIGIRKYAYIFSGSLFLIIFISLGVRGLNQGIDFTGGRSYVVRFDDAVSTTDIA
                     SDLKHVFNEAPEVKTFGGQNQVKITTKFKIDSKDENIENEMDSLLYVGLKPYLQDGTT
                     LKDFQTTYKQSSIKVGPTIAQDIKRDAVIAVILALIVIFLYVLIRFRWWSYSTGAIVA
                     LFHDTMMIVGMYSLLYFRVPFGLEADQSFIAALLTIIGYSINDTVVIFDRIREYNKLY
                     PKRSSRDNINNAINDTLSRTFVTSFTVLIVLVPMFFLGGEVIRGFIFALIIGTISGSY
                     STVFIATPIAYDLEKAVEKRKNRKIKQ"
     gene            complement(13564..13637)
                     /locus_tag="PK839_07330"
     tRNA            complement(13564..13637)
                     /locus_tag="PK839_07330"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13601..13603),aa:Arg,seq:ccg)
     gene            complement(13827..14324)
                     /locus_tag="PK839_07335"
     CDS             complement(13827..14324)
                     /locus_tag="PK839_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005830374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOG72583.1"
                     /translation="MSQQEFNTALVELEPNLERFAYSLTANREDANDLLQETYLKALT
                     YKDKFEDNTNLKAWTFTIMKNTFINNYRKAVKQNTTFDSSENQFLLNSSYEKNGPESM
                     YSHSEISKKIDQLEDEFRIPFQMHTSGYKYKEIAEKLNLKIGTVKSRIFFSRQKLMSA
                     LRDYE"
     gene            14721..16046
                     /locus_tag="PK839_07340"
     CDS             14721..16046
                     /locus_tag="PK839_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOG72584.1"
                     /translation="MAKKSKKIFVLDTNVILHDYHCIYNFQENDIVIPIVALEELDYF
                     KKGNEMVNYNAREFVRELDKITGDNLFNGGVSLGKNLGKLRIETGKPMPKAMLDSFAE
                     ATPDHRILAITYHLASVNPDRPVILVTKDINLRMKAKSLGIVAQDYLTDKVQDIDSLK
                     KEIEIIDGVDDEFIQKLYENPSELGAAPLKVKPYANQYLILKGNKSSALAHFDKATNM
                     LNRVEKTRAYGIEPRNSEQTFALDALLRSEVKLVALTGKAGTGKTLLALAAALQQEEN
                     FNQILLARPIVALSNKDIGFLPGDINEKISPYMQPLFDNLSVIKNRFKPASKEYMHIE
                     EMLRTEKLVITPLAYIRGRSLSNVYFIVDEAQNLTPHEIKTIITRAGEGTKFVFTGDI
                     FQIDHPYLDMKSNGLTYLSDKMHGQELFAHINLVKGERSYLAELASNLL"
     gene            16154..16639
                     /locus_tag="PK839_07345"
     CDS             16154..16639
                     /locus_tag="PK839_07345"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOG72585.1"
                     /translation="MKRDLYLLRHAKSVERGSEMRDADRALKPRGIEDVLNLAGRVHA
                     KAKSIERILSSPANRTIHTAILFAEATGIPMDRIAICSDIYLADDDDLALIVSEADNQ
                     LTSLMIVGHNPSVTNLANRFLNEPVFDVPTSGLVVLQFDADSWMNISKSTLTDSFTVF
                     P"
     gene            16755..18854
                     /gene="ppk1"
                     /locus_tag="PK839_07350"
     CDS             16755..18854
                     /gene="ppk1"
                     /locus_tag="PK839_07350"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HOG72586.1"
                     /translation="MGRKNLPLVNREISWLSFNGRVLQEAEDKNVPLIERLKFLGIFS
                     NNLDEFFRIRVATLRRLLVVEKGAKKVMGDNPRKILEKIQRLVVSYQEKFDSIYSDVK
                     LQLRAEGIYIMDETELDPDQVDFVRSLFKRKISMSLGPIMISRLDRFPQLVDQSIYLA
                     VKLSSSENRQAKEYSLIEIPTRNISRFIRLPRKDNQEYIILLDDVIRLCLDMVYSILP
                     YDRFEAYTIKITRDAELDVDNDISESLLDKISKGVKNRKRGEPVRLVYDEKIAPDLLN
                     FIVKGLSLDEKDYILPGGRYHNFKDFMDFPNFGRSDLVNAPMKPVQVAELENASSILG
                     VMAKHDFILHYPFHDFAYYVKMLREAAIDPKVTAIYITLYRMAAESRVVNALMNAAQN
                     GKKVTAVIELRARFDEHANIYWAKQMQEAGVNVVFGMPGIKVHSKMTLIIRHEGRKDV
                     RYAVVSTGNFHEGNANLYTDVNLFTVNQGIAREVEKVFTFLEFNYKTFSYKHLLVSPV
                     NMRRKLYSLIENEMDNAAKKLPAYIHCKINNLVDTEMIAKLYQASQAGVEIKLVVRGI
                     CSLVPGVKGVSDNIEVFSIVDRLLEHSRIFIFANSGKETCYISSADWMTRNLDYRVEI
                     ATPIYDKHLISELKTVINMALKDNVKSRVIDQKQSNSFRNPEAGEEPFRSQLELYRYY
                     KSKENLQSAATEDYNLT"
     gene            18954..19847
                     /locus_tag="PK839_07355"
     CDS             18954..19847
                     /locus_tag="PK839_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008860924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HOG72587.1"
                     /translation="MKLLKFAAIDIGSNAVRLLLSNVVENGDSVVFRKSSLIRMPIRL
                     GEDAFETGIISEARIQKLIKTMIAYRNLMEVEDVVAYRACATSAMREALNAPEIVERV
                     SRQTGVNIEVINGRQEAEIVYSNGIAETLDSSKPFIYVDVGGGSTEISLFQNGQNIAS
                     QSFDIGTIRMLKEKVGKSEFDLIKKWLKNLKITGGKPKIIGSGGNINKLYRLAGKVEG
                     RPLSFAELQKLFAQLKSYTFEERISILGMNPDRADVIIPAARIYLKTMEWTGASKILV
                     PTIGVSDGIVRMLYKNYRSRS"
     gene            19975..21282
                     /gene="mtaB"
                     /locus_tag="PK839_07360"
     CDS             19975..21282
                     /gene="mtaB"
                     /locus_tag="PK839_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HOG72588.1"
                     /translation="MVEQRKRVAFYTLGCKLNFSETSTIARQLVEMGYERVSPDDKAD
                     VYVINTCSVTEHADKKCRQAIRKFASRSPGALIAVVGCYAQLKPDEVAAIEGVDLVVG
                     ASAKFQLPQLLATHVGKDGAKVYSCDIDRVADFSPAYSSGDRTRSFLKVQDGCDYHCS
                     YCTIPLARGKSRNMPVTNIVAQAQAIAAQNVKEIVLTGVNTGDFGRSTGESFIELLKE
                     LDAVEGIERYRISSIEPNLITDEIAHFVASSKKFLPHFHIPLQSGSNRILGLMRRRYN
                     RELFAEKIAMIRRLMPDTFFGVDVIVGFPGETEADFMDGYSFIREVNPAFLHVFPYSE
                     RPNTAAALLPAKVDAREIKVRADRLGALSSELHRQFYISRTGQVHHVLLEASQKSGLM
                     FGFTENYIRVAVPYNRNLAGKVVDVRLAALNPNGFMEGELMQP"
BASE COUNT         5677 a         4842 c         5054 g         6100 t
ORIGIN      
        1 atgccgggct acgatgataa ttcgagcctg cggccaaata tttacctgca gtttggtctt
       61 agcgatttcc ccaaccatag cgtttcgggt ttaattttca gcaacgactt accggttgca
      121 atcccgggtg ccaatatctc catgcaggga tttctaacct acaacgccac aactaatgag
      181 gacggaagat tctctattga cagcctgctt gttgatagct acgttctaaa tgtgtctggt
      241 aagggttttg taagctactc ggacactctt accgttgatg gcgacatgat gctggatacc
      301 ataaagttgt cgagcgccag ctatcctgta actttcagtg tatatgccag cggtgaagct
      361 gttgaggggg ctgaaattgc cattgaggga tacccggtta tctatacgaa ctccctcggg
      421 gttgcactta ccagccttaa agatggagat tatagctaca atgttaccaa aaccggctat
      481 aacccaattc tggggaatac atttactgtt tccggagggg ataaaaacgt agatatttta
      541 cttttccccc tgtcggctgg cgattccaaa ctaaatgcag cagcagctta ccccaacccc
      601 tttgacagct acttacggat aggcggcgca gcagaggtga aatatatcaa gcttacaagc
      661 cttaccgggc aggtggtgat ggagtttaga ggaaacggat cggaaaccta tacactcaac
      721 acagctaacc tgccggccgg attttacctt attaccatgg agtcagccag cggcgaaaag
      781 ctggtgaaaa aggtaatcaa gcagtagtag atgtatttca cacccattgg ggttgaatct
      841 taggtgttga aataaaatga aaacgaccgg ggaacccccc ggtcgtactt gtacaagtaa
      901 aaaattaaat caatcttaaa tgcagcgcag gtatcctact taacctccca cgtttcgccg
      961 ctgttgagca ggtcgtcaac gcatttgatt tttgcatcct tcattacctc cagcacctgg
     1021 tcacgaacat tatcgtcgta ggttgaatcc tttacattcc tgataacgcc aagagcaatc
     1081 ggatattcag gaaactccat gttggccagc atcatgtgaa cgccggggtt ggggttatag
     1141 gcatcatgaa ccaggatatc atccatggtg aaacctccct gtccaatggt taccaccttt
     1201 aggctcaacc cgctgagcac tagtccctta tcccgattct tgccaaaaac catcttttcc
     1261 ccgtgatgaa ggacaataac cctgtcgtcc ctggtttcct tgctgctgat gaagtcgtaa
     1321 gccccatcgt taaaaattac gcaattctgt agaatttcta ttaccgatgt gccgtcgtgc
     1381 ttagcggcct caaccataat ttcggaggtg agcttaacat ccacatcaag ggtacgggca
     1441 aaaaactttc cctgtgcgcc aataaccagc tcaccaggcc ggaatgggtg ctcaacagtg
     1501 ccaaaaggcg atgttttggt gattttgcca agtggcgagg tgggggagta ctgacctttg
     1561 gttaaaccgt agatttggtt gttgaatagt agaatattca cgtcaatgtt gcgtcgaatg
     1621 gcgtgaatga agtggttgcc accaatagcc agggcatccc cgtcgccggt aatctgccaa
     1681 acgctaaggg tgggattggc aactttaaca ccggtagcaa tggccgaggc acgaccatga
     1741 ataccatgaa agccataggt atttacgtag taaggaaagc ggcttgagca gcctattccc
     1801 gaaacaaata cgtagcgctc tatgttgtac ttcagctctt ttgaaacctc gggtagcgct
     1861 ttttggactg cactcagaat ggcgtgatcg ccacatccgg ggcaccagcg cacttcctgg
     1921 tcacttttga aatcctgagg tgtataattt aagtactgtt cttccatggt tattttccct
     1981 ccagtaattg gttaaacttt tctaccagct caaccacggt aaagggtaac ccttgaacct
     2041 tgttgaactg caggtagttg aactcctgaa aattcatacg aaggtagttg gcgaactgcc
     2101 ccatgtttag ctcgcaaacc accagcttct tatagcggct cagtatatcc cgggtatttt
     2161 ttggcagtgg gtttatatag ctgaagtggc aaagcgaaac gcttttcccc tttccgcgca
     2221 ggtcggttac ggcatcgagc aggtggccat aggtgccacc ccaaccgata accagcaggt
     2281 cgccttcgct atcgccaatc accttttgtt cggggataaa ctccctgacc ctctctacct
     2341 tggcagccct aatatccacc attcgctgat ggtttattgg gtcgtgcgaa agcgaacctt
     2401 tcagaaagtc tttttcgagg cccccaaccc tgtgggtaag accttttttc ccgggcagcg
     2461 cccagcctct ggcaaggcgc ttatcatcgc gtgcgtaggg catgtaaccc tgggtgcctt
     2521 cctctaccaa gggagggtta atttttgggt actccttcat gttggggata agccatggtt
     2581 ccgaaccatt ggcaataaag ccgtcggtta gcaggataac aggagtcatg tgctccatgg
     2641 caattttacc agcattaaac gccgcatcaa aacagttgct tggggaggtt gccgaaatta
     2701 ccactactgg tgcctcgcca tttcttcccc atagcgcctg cataaggtcg gcctgctcgg
     2761 ttttggttgg taaacctgtt gaaggtccac ctcgttgaac atccacaacc accaggggca
     2821 actcggtcat tactgctaac cccaaagctt ccgacttaag tgatagcccg gggcctgaag
     2881 tcgaggtaac ggcaaagttg cctgcaaagg aagcaccaat ggcggtgcaa atgccggcaa
     2941 tctcgtcttc ggcttgcagg gtttttaccc ccaaatcttt tctcttggaa agttcagcca
     3001 aaatatcggt tgccggagtt attgggtatg aaccgcagaa gagaggcagt ccggcttttt
     3061 cggcagcagc tattagcccc catgccgtag cggtgttacc gcttaccgtt ctgtattttc
     3121 ctttaggcag tatttttgat ggtttaatac tgtaagtatt tggaatggcc tgaatatttg
     3181 ctgcgtagtt gtagccgtcc tgaagtactt ttttgtttgc ctcaataatt tcgggcttct
     3241 tcttaaactt cgtttcaaag tatgtttcgg tgaagttcat cggacggtcg aaaatatagt
     3301 aaaccatccc cagcgcaaac atgttcttgc tcctaacaat gctcttatta tccaggccgc
     3361 tatccttaag gctttccttt gtaagagtgg taattggtgc catgattacc ttgtagtcct
     3421 gtaggttgag ctcggttaca gcatccaggg ttttataacc ggcacgctgg tagccttttt
     3481 cattaaactg atcggcatcg gcaatcacca tgcctccttt tttgacccat ttaatgttgg
     3541 ccttcagcgc ggcagggttc atagcaacca gtacatcgca ataatctccc ggggtgttaa
     3601 cctttttact cccaatatga acctggaaac cagaaacgcc gccaatagtc ccttgtgggg
     3661 ctcgtatctc ggcaggataa tcaggaaatg tgcttacatc attgccaaaa agtgcagagg
     3721 catcggcaaa aagcgttccc gttagctgca taccatcacc ggaatcaccg gaaaatcgga
     3781 cagcaacctc atccaactct tgaacacctg tttgtttctc catagtaata agtttattac
     3841 gcctttaact agtacctaat tttataattt gtataacaat aaattactaa aaatgctcac
     3901 tttcttagga attataagtt atgctttgca aaattaccct ttttattatt aggcaataaa
     3961 attaaataat atttaataac atatcttttt gggtgaatgc ctttttcacc tttttttaat
     4021 cattcatgat gtgggccatg cctgccaaat aaattcagta ttcccttata ttgcgatccc
     4081 tcccagtata tttgtccgca ccggctgcaa cggtaaaatg agtggtaatg gttataagag
     4141 ttttcggcaa caagaccttt aacattttcc ctatcaacag tttctactaa accgttacat
     4201 agcgagcagc gagagaaggg gtcagcaaag caagccatgc tgaagtaatc aactacctcg
     4261 ttgagctgct ccttgggttg agtcgagcga acatagtagc catggatggc tgctccgctc
     4321 cggagaattc ccttgtcgcg tgtaagaatt attctattct ctgcggctgc aagcctgaga
     4381 atttggtcgt cggagtagca gttgctatag agcgtgtcga agccatatag tctaaggtag
     4441 gatgccagac gccccaggtg aacgtcgagg atgaataccg gtggggcggg ctcgggtggt
     4501 cgtagtattc cgtaatctat gctaaggttg taaaatcgcg ggtagcatga aatttgggag
     4561 ctgggttcag gcttgtagtc aaaaccaacc gatatgctgt cagccacaat taaaaatacc
     4621 tctacatgcg gtactccaac cgattcgata aggtccttaa ctgtgggtgt tcccatgtag
     4681 ggtaggttaa atggaacctg ccgtttcgac gccggtaaaa aatggtttaa accgccatag
     4741 aagcgcagct cgatatgctt tgagaatttc tttaccattt taaatttatt cggtgcaacc
     4801 tggcagattt taagggattt gtaaaccaat ttccccattc catcctgtta aacaggtgaa
     4861 tttttacaac aatagttttc attgctattt ttgtgaaaaa atacactaaa gtatggcaat
     4921 aatcaaacca cttaggggat ttactccaaa aattggagaa aactgtttta ttgccgaaac
     4981 ggcagtaatc ataggagatg tagaaattgg cgatgactgt agcatttggt atggcgttgt
     5041 ggctagaggg gatgtgaact ccatccggat tggcaaccgg gttaacattc aggataacgc
     5101 ggttttgcat accacctacc aaaaaacaac aatcaggctg ggcaacgatg tatcggttgg
     5161 ccatggtgca attgttcatg gtgccgaagt tcgcgatggt gcgctgctag gaatgggatg
     5221 cacggttatg gataatgcgg ttattggcga gggtgcaatt gttgctgcta atgcgcttgt
     5281 tctggaaaat actactgttg aaccggggac aatctacgga ggtgttccag ccaagtttat
     5341 caaaaaggtt gatgcggagg ttagccgtga gatgaacgag cgaattgccc gtaactacct
     5401 gttttatgca ggatggtata agcaaggata aaggattcaa aaacttgttt gcctaccggc
     5461 ggaacttgtc cggctccggc tggatcaatt ttaattccct tattcttctt tcttcttgcc
     5521 gcctagcggt aacaacttta cttttcccgc tctacgtttg gccataaata tgtagatttg
     5581 caacaatttg tataaacctt ttcacccaat gagtaactat aaaagatatc ttattacttc
     5641 tgcgttgccc tatgccaatg ggcctgtgca tattggacat ctggcgggtg tttacatccc
     5701 ttccgatatt tacacccgct atcttagagc aaaaggcgca gatgttatat ctgtttgcgg
     5761 ctccgatgag catggggttc ccataaccat caaggccagg catgagaata caactccaca
     5821 ggctgttgtg gataagtacc atgccataat aaaagaatca ttcaaacagt tcggaattga
     5881 tttcgatata tactcccgca ccacttcgcc tgttcactac gaaactgcca gcgaattttt
     5941 taagaagctc tacgaaaagg gtgtttttgt tgaaaaggtt accgatcagt actacgatga
     6001 ggaggtgggt caatttctgg ccgacaggta tattaccgga acttgcccgc attgtgggaa
     6061 tcccaacgct tatggtgatc agtgcgaaaa gtgcggaact agccttaatg ctaccgatct
     6121 gattgaccca aggtcaaccc taagcggttc caagcctata ctgaaagcaa ccaggcactg
     6181 gtttttgccg ctagacaaat ttcaacccga gcttgaaaag tggatacttg aagaccacaa
     6241 ggagtggaaa gccaatgttt acggacagtg taaaagttgg ctcgaccagg gtttacaacc
     6301 ccgggcggta agtcgcgacc tcgattgggg tgttccggtt ccgctggatg gtgccgaggg
     6361 aaaggtgctt tacgtttggt tcgatgctcc tattggctat atatcggcaa ccaaggagtt
     6421 gaccccaaac tgggagaaat actggaagga tagcgatacc cgtttaatcc attttatcgg
     6481 taaggataac attgtttttc actgcattat tttcccctcc atgcttaaag catacggaga
     6541 ttacattcta cccgataatg tcccggccaa tgagtttctt aacctggaag gcgataaaat
     6601 ttccacctct cgcaactggg ctgtttggct gaacgagtac ctggttgagt tccccggcaa
     6661 gcaggatgtg ctgaggtatg tacttaccgc caacatgccc gagaccaagg ataacgattt
     6721 cacatggaaa gattttcaaa cccgtaacaa cagcgagctg gttgccatcc tgggaaattt
     6781 tgttaacagg gctatggttc ttacccataa gtacttcgat ggcaaactgc ctgccagaaa
     6841 cactcttacg gattacgata attcagtttt gcacgaattg ccccgtataa aggaaaggtt
     6901 ggagcacaac cttgaagagt ttaaatttcg tgaagcgctt aaagaagcca tgaatattgc
     6961 ccgtttgggc aacaagtatc tggctgatac tgaaccatgg aagcttatgg agaaggatcc
     7021 ccggcaggtt gccaacattc ttaacattgc catgcagatt acagcgaacc tttccattat
     7081 acttgaacct tttctgccat ttactgcaga gaagatacgc aagatgatta attttcagag
     7141 taaggaatgg aagcttgctg gtttagatag cctgctggcc ccggggcata ttcttgacaa
     7201 accccagctg ctattcgata aaattgagga tgatgttatt gctgtccagc taaataagct
     7261 tcaagccacc aagctggtaa accagaattc tgttaaaaat gttccggaac aaaagggttc
     7321 tataagctac gacgattttt ctaaaatgga tattcgggtg gcaaccgttc tcgatgctga
     7381 aagagttcct aaaactcaaa agctgcttaa acttaaagtt gatacgggaa ttgacgtacg
     7441 caccttggtt tccggaattg ccgagtactt cacccccgag gagatggttg gacgacaagt
     7501 gctagtttta gcaaatcttg aaccgcgaaa aattagggga atagattcgc acggcatgat
     7561 tctgatggcg gaggatacaa gtggaaagct acagctggtt tccccggcag cgaaggtttc
     7621 taatggctca actgtaaagt agaaatacta ttctttagga ataggtgctg gctacttttt
     7681 gttgtttagt agtagcgcct attttcttac aaccggttca ccctctactg acttaaaagc
     7741 cataattttt caaatgtttg ctctgatttt aatctttcca gctcccgcaa agccctgtcg
     7801 cggttgctaa aagtatagag cgaaacgctg tagagcttgt tacgtggtat aagggttggt
     7861 ttgtagccct tttctgccag ctcactcagg aacttctggg cattaacctc gcttgtaaag
     7921 ctgccggcaa ttatgtagaa agatttaccc gtttcaacca ccggtggctc tgtgtagcta
     7981 agcgctactt tcttactggt ggtttgttcc agaatctttt cggctccacc ctcatcggtg
     8041 gcattaggtt ggcctgaggc cgatatgcta tcgggcactt gcgagtcctc ttcttctacg
     8101 atggaatccg gaatggcaga agctaaagga aggttaccta cggatgccat gctggacgtg
     8161 ctatcggttg ttgatggaga aaccgaggtt tgcgattgct cccaaaaatg caatgctgga
     8221 gtaaaccaac acactgctac tgctaccacc actacgcagg ctgtaatggg gacccataac
     8281 ctaacccgcg atttttttgc aacagctggc tttggagtcg gaattttggt ggtttgctct
     8341 gttgatagtg gactggacgc ctgggtttcg gctacagctg cttttttgtt ttccttcgga
     8401 ctggttagtt tggagtctgc tttttgctta gggagcactt ctattgctga aagtccatac
     8461 gactcggttg cagctgcgct ggcatcaaat tcaagagtga tattgttgtt gtcatccttt
     8521 aacaaagttc caacaccggg gaactcgaaa ggttttctac tgctaagtgc attgtttacc
     8581 ctctccacaa gttgcgttac ccttaattct gcttccttat gatcaattcc cagtgtgctg
     8641 cacaggtagt ttgtaatagc ctcgtcgtca aacgtgcgcg atgtgtcaaa aatgtagtac
     8701 tcgcttgggg gttcaatgct tgacctatcg tccgatactt ttgccggatt acgacgttta
     8761 acaaaactgc ctaatccagg taggctgaca aaatcgaact gtgacagaag cagctgaatt
     8821 acttgctcta attccaactt gtagggtagt tttgggtgaa cgaacaaatt tataaaatta
     8881 atcgtgttaa tataacatcc tgctggtaca attatttttg aatggggctt ttccgcttga
     8941 aaatagtagg atgttttctg cttgtctgct atggttggct tgccaggttt taggatgttt
     9001 ttttgttggg tggattgatt ttaccaccta actgttcatg ttgctctcgc aaatcgtttc
     9061 ctaaaaattt cggaaaccaa taatctgcct tacctcgtta atggttttgg atgcgctctc
     9121 acgcgctttt tctgctccat ggtgtgctac ctggcttagg taggcatcgt ttgccagaat
     9181 ttcctcaatt ctttccctta ttggcgtggt aatccgtatg atatcctctg ccagctgttt
     9241 tttcatatcc ccgtagcgaa tatggcatga gctgtattgc tcctcaaagt agctgtacgt
     9301 ttcaggatcg gaaactaccc gcatgagggt aaaaaggttt tttaccggct ccgatttttc
     9361 ctggtttggt tgggtgggtc ccgaatcggt aactgctttc atcaccttgc tacgtatggt
     9421 ctttgggtcg tcggacaggt atatggcatt accttccgat ttccccattt tacccgaacc
     9481 atcaagcccc ggtattttta cgagctcctt tccaaagttg tatgccactg gttcggggaa
     9541 gtaatccacc ttgtagagcc tgttaaatcg gttggcaaat gtgcgggtca tctccaggtg
     9601 ttgctcctgg tcctttccca ccggaaccat ggtagccttg tggataagaa tgtcggcggc
     9661 cataagcgtg ggataggtga gcagcccggc atttatgttg tcggggtgtt tacgtatttt
     9721 ttccttgaac gaagtgcatc gctccaactc tcccacgtaa gcgttcatgt tgagtaaaag
     9781 gtagagctcc ggaacctggg gcaaatcgct ttgcacgtat agcgtggcaa cctcggggtc
     9841 aagcccacat gccaggtact cggctaaaac ctgcttaacc gatccatgca aatctgtggg
     9901 tgttgggtga gtggtaagcg agtggtagtc ggcaataaaa aagaagcact tgtgctcgtg
     9961 ttgcatttga acaaagttta gcaaagctcc atagtagttt cccaaatgaa gcttgcctgt
    10021 tgagcgtatt ccgctaagta caatttccat ggcttgtggt gcgtaggtta tgtttaacta
    10081 caaaaataca actttatgcc gtacaatttc catgttgaat ggcagggagc gatgcagcaa
    10141 tgttgatata aggtagtatt ttgatattga agcaattact ttgggttgat ttgtggctgc
    10201 tacggatttt atttacttta acctctacta aaaaaaaact caaccctgcg tgggttgagt
    10261 ttttaacaat gctcctaatt attgctacgg gattttactg tttaattttc ctgtttttac
    10321 gtttttcaac agctttttca aggtcgtagg caataggtgt tgcaatgaat acggtagagt
    10381 atgacccgct tatagtacca atgattaagg caaagataaa gcccctgata acctcgccac
    10441 caaggaagaa catgggaact aaaacaataa gcacggtgaa ggaggttaca aaggttcggc
    10501 tgagggtgtc gttgatagcg ttgttgatgt tatcgcgtga tgaacgcttg gggtagagct
    10561 tgttgtactc cctgatacgg tcgaagatta caacggtgtc gttgatggag tacccaataa
    10621 tggttagcag ggcggcaata aacgactggt cggcctcgag cccaaagggc accctgaagt
    10681 acagcagcga gtacattccc acaatcatca tggtatcgtg gaaaagcgct actatagcac
    10741 cggtgctgta gctccaccag cggaaacgaa taagcacgta gaggaatatc acgattagtg
    10801 ccagaataac cgcaattacc gcatcgcgct tgatgtcctg tgcaatggta ggccctacct
    10861 ttatggagct ttgcttgtag gtggtttgaa aatccttcag ggtggttcca tcttgcaggt
    10921 agggttttag cccaacgtag agcagggagt ccatttcgtt ctcaatattt tcatcctttg
    10981 agtctatttt gaacttggtg gtaatcttca cctggttctg acccccaaat gttttaacct
    11041 cgggcgcctc gttaaacaca tgctttaggt ccgaggcaat atcggttgtc gaaaccgcat
    11101 cgtcaaacct taccacatac gagcggcctc ccgtaaaatc aataccctga ttcaaccccc
    11161 ttacacccag cgatataaat attatcaaaa aaagtgaacc ggaaaagatg taagcgtact
    11221 ttcgtatgcc gatgaaatca atatgtagat ttctaaaggc atgctcggta aacttaacgg
    11281 agtaccggat gttgatgtta tggtttatca tccaggtttg aataagcctg gagataagta
    11341 ttccggtgaa aagggaggta atgataccga tgatgagcgt ggtggcaaaa cctcgaattg
    11401 ggcctgtgcc aaatacgtag agaataattc cggttagcag ggtagtaacg ttaccgtcaa
    11461 taatggcaga gagggcattc ctatacccct cgttgactga gggtatagtc ccttttcccg
    11521 atcgtaattc ctcccttatt cgttcaaaga tgagaacgtt tgtatcaacg gccataccca
    11581 gcgttagcac aatacctgcg attcccgaaa gggtaagcgt tgccccgaaa gaggccatga
    11641 ttcccatgat gaagaaaagg ttgaaaaaca aggcgatatc cgcaaccaag cctgccttga
    11701 agctgtacca taccatcatg aagagcagca cgaggataaa ggagaggata aaggagttca
    11761 tacctgaatt aatagactcg gttcccagcg atgggcccac cagctcttcc tgaacaatgc
    11821 gggcgggagc cggcattttt ccagacttta aaatgttcgc aaggtctttt gcctcgttga
    11881 tggtaaaatc accggttatt tgtgaacgtc cacccttaat ctcgctctgc acccttgggt
    11941 aggagtaaac gtagttatca agtacaatag ctatacagcg accaatattt tcaccggtca
    12001 tgcgcgccca aactcttgcc ccttcggcat tcatggacat gtcaacttcg gcgctaccct
    12061 ttgtctctcc aaacgcctct ctggcgtcgg ttattacacc cccatcaagg ggtgccttgc
    12121 catcgcggct ggttaccttg agggctataa gctcgtagag cgactcggat ttatcccatt
    12181 ttggaggttt tacaccccac aaaaatttca cgtcaacggg gaaaagtgcc ttaatcttag
    12241 gcatccttag tatttcgtta accctggctg tgtcctttac atgggcgtag cctaccaaag
    12301 gcccgggggc gggctgatcc tggtacaggt tgggttgaag caggctgaat aacgggtatt
    12361 tttttgtctg gtcggcctga gtggctgaaa gggaatcctc ggttgaggtg gctttcagct
    12421 tggccagcag ctcatctgtt acggtatcct ttttggcggt tagctccttg ttgctggtgc
    12481 ttttaatgga atccgtctta gccgtagcat cctgaatctc agccaacgcg ttgttggctg
    12541 caataaagtt gttgaaaagt tcgctaaact cgtaggtttc ccagaattcc aggttggctg
    12601 tcccctggag tagctttcgt acacgttcgg gttcctttat accgggaagt tcaatcagaa
    12661 taagacccga gttttccagc cgctggatgt tgggttgggc aacaccaaac ttgtcaattc
    12721 tattacggag gatgttaaag gagttgttta tggcattatc tgcctcagct cttaaaatct
    12781 tgataacctg atcgtttgta gattcaaagt taatcttatc ccgcagttct ggggtgctaa
    12841 aaattgcagc cagccttgca tttggtgcaa cctgttgaaa agctttacca aattttgtta
    12901 caaaatgctc gctgccgggc attttctggg ccacctccat ggctttcagg aatgagctat
    12961 cctggctgtt gttcgatagg gagcgtatca ggtcggtaac cgatacctcc agcatcacgt
    13021 tcatgccacc cttaagatcc aaccccagat ttatttcccg ctccttgcac tccttatagg
    13081 tgtacttgcg gatgtacagg aagttgtaaa caggctcgga cgaaatggaa tccatgtagt
    13141 gggtttcctt agccctgtct atctcgttgc ccaccatcgc atactcgcgc gccttttttt
    13201 ctactcgcct ggtaaccaac gtgaatgaca gctggtatat gcaaaccaat attaaggcta
    13261 tggcaataaa tcttatcgca cctttgttct gcatcttttg aagtattaag taggttgtat
    13321 gatttttaga tgtataaaat gacgggcaaa tatattattt tttaatcaaa ataattgcta
    13381 ttcattgttt aacctctaca atatgccgat tcttagaagt ctgctggcat ttttagatgg
    13441 gcaatgcggg cttgaaagct atcgcccgat gttggtttat catggttgag cagaaacgct
    13501 ctagcgaatg ttgggagcat gttgttaaag taaaggtcaa ccactacagg ttgacctttt
    13561 ctgtgacccc ggcgggattc aaacccacga ctttcagaac cggaatctga cgttctattc
    13621 agctgaacta cggggccatg tcaggctgca aatatagtgg agttttatgc taattccaac
    13681 ttttacccac atgatattcg ataagtatca gcctttgagt gttttctaaa tgtttccttc
    13741 gttttaaaaa ggttttgact aaaaattgca gaagccatca atctccgatg gctcctgcta
    13801 cctattatgt gttgttgcct agcaccttac tcgtagtccc taagggcgct catcaacttt
    13861 tggcgtgaaa agaaaattct gcttttcacc gtgccgattt taaggttgag cttttctgct
    13921 atttccttgt acttgtagcc cgaagtgtgc atctggaatg gaatccgaaa ttcatcttca
    13981 agctggtcaa ttttcttgct aatttcgctg tgggagtaca tggactcggg cccattcttc
    14041 tcgtagctgc tgttgagcag gaattgattt tcgctgctgt cgaatgtggt gttttgctta
    14101 accgccttac ggtagttgtt gatgaaggta tttttcatta tcgtgaaggt ccaggccttt
    14161 aagttggtgt tgtcctcaaa cttatccttg taggtcagcg ccttcaggta ggtttcctgt
    14221 agcaggtcgt tggcatcctc gcggttagct gttaagctgt aggcaaatcg ttccaggttt
    14281 ggttccagct ccactagtgc ggtattgaac tcttgctgtg acatatttgt aggtttttag
    14341 ttactattcc ttggttaaac ttcacgacaa agttagaatt agtctaaata aaaatcaagt
    14401 tgataattat caactcacgc caacatttaa cttaattcta aatagaaaat tggacaaaat
    14461 gcctgattat taatggcata tagttgtttt tataaattgt gttcaattcg atattttata
    14521 tggtgtttta agttaatggc tggccaaaga ttatcacccc tcccccggag atgattatat
    14581 gcgctggcac acaattattt acaataaatc gggaaaggag agtagtgttt tttagctcaa
    14641 ttatttagat aggtgataag caattcaagc taaaacagtc tatttgtgtt atatttaaag
    14701 gttcattaaa tcaacagatc atggcaaaga aaagtaagaa aatattcgtg ctggacacca
    14761 atgttatcct ccacgactac cactgcattt ataattttca ggagaacgac attgtaatac
    14821 ccatagttgc gctggaggag ttggactact ttaaaaaggg gaatgaaatg gttaactaca
    14881 acgcccgtga gtttgttcgt gagctggata aaatcacagg tgataaccta ttcaacggag
    14941 gagtttccct ggggaagaat ctcggcaagc tacggattga aacagggaaa cccatgccta
    15001 aagccatgct cgactccttt gccgaggcta ctcccgacca ccgaatactg gccattacct
    15061 accatttggc atctgttaat ccagacaggc cggttattct ggttaccaag gatattaacc
    15121 tgaggatgaa ggcaaaatcg ttgggaattg ttgctcagga ttacttaacc gataaggttc
    15181 aggatattga ctccctgaaa aaggaaattg aaattattga cggggttgac gacgagttta
    15241 tccaaaaact ttacgaaaat ccaagcgaac taggtgctgc accgcttaag gttaaaccct
    15301 acgccaacca gtaccttatt cttaagggta ataaatcgag cgcactagcg catttcgaca
    15361 aggccaccaa catgctgaac agggttgaaa aaacgcgtgc ttacggcatt gaaccccgta
    15421 actccgagca aacttttgcc ctagatgcct tgttacgttc ggaagttaag ctggtggccc
    15481 ttacaggaaa agccggcaca ggaaaaaccc tgctagccct ggctgcagct cttcagcagg
    15541 aggaaaattt caaccaaata cttctggcca ggcccatagt tgccctctcg aataaagata
    15601 tcggtttcct gcctggcgac atcaacgaga aaatcagccc atacatgcag ccacttttcg
    15661 ataacctttc ggtgattaaa aataggttca aacctgccag caaggagtac atgcatattg
    15721 aggaaatgct tcgcaccgag aaacttgtca tcaccccgtt ggcctacatt aggggacgaa
    15781 gcctgtcaaa cgtgtacttc atcgttgacg aggcgcaaaa ccttactccc cacgagatta
    15841 aaaccataat tacccgggca ggggagggaa ctaaatttgt tttcaccggc gatatatttc
    15901 agattgacca cccctacctg gacatgaagt cgaacgggct aacctacctt tccgacaaaa
    15961 tgcacgggca ggagctgttt gcccacatca acctggttaa gggcgagcga agctaccttg
    16021 ccgagctggc cagcaacctg ttgtaataga tttgccctta cttcagaagc aactttccgg
    16081 gtatcaactt ctgaaatcgg ctgggcatcc ttacatgtaa ttgagagtag gtttattgtg
    16141 aaagtaatga ggtatgaaaa gggatttgta cttgcttcga cacgccaagt cggtagaaag
    16201 agggtccgaa atgagggacg ctgatagagc actgaaacct aggggaattg aagatgttct
    16261 taaccttgca ggaagagttc atgcaaaggc caagagcatt gagcgcatac tttcgagccc
    16321 tgctaaccgt accattcaca cggctatact atttgccgaa gcaacgggca tacccatgga
    16381 caggattgct atctgttccg atatctatct tgccgacgat gatgatctgg cgcttattgt
    16441 ttctgaagct gataaccagc ttacctcatt gatgattgtg gggcataacc cttcggttac
    16501 caacctggcc aaccgatttt taaatgaacc tgtttttgat gttccaacat ccggccttgt
    16561 cgttcttcag tttgatgcag atagctggat gaatatttcg aaatcgacac taaccgatag
    16621 tttcaccgta tttccctgat acagggttaa tttgctacga tatcagcata aaccttaaca
    16681 tttagataac atttcgttta cgcattgttc gaaaaatact tcttaaaatt gaacttttta
    16741 acctaaacta agctatgggc cgaaaaaact tgcccttggt aaacagggag attagctggc
    16801 tttcattcaa cggacgggtg ctccaggagg cagaggataa aaatgtgcca ttgatagaaa
    16861 gattgaaatt tctaggcatc ttctccaata acctcgatga gttttttcga atccgcgtgg
    16921 ccacgcttcg gcgtttgctg gtcgttgaaa agggtgcaaa aaaagttatg ggtgataacc
    16981 ccagaaagat actagaaaag atacaacgcc tggtggtttc gtatcaggaa aagtttgatt
    17041 ctatctatag cgatgttaag ctgcagctga gggctgaggg tatttacatt atggatgaga
    17101 cggagctcga ccccgatcag gttgattttg ttcgctccct gtttaaacga aaaatctcca
    17161 tgtcgcttgg tccaatcatg ataagccggc ttgaccgttt tccccagctg gttgatcagt
    17221 ctatttacct tgctgttaag ctctctagta gcgaaaaccg tcaggccaag gagtactccc
    17281 ttattgagat tcctacccgt aacatttcca ggttcattcg cctaccccgc aaggataacc
    17341 aggagtacat tatcctgctc gacgacgtaa ttaggttgtg cctggatatg gtttacagca
    17401 tcctgcccta tgataggttt gaagcctaca ccatcaaaat tacccgcgat gctgagctgg
    17461 atgttgacaa tgacatatcg gagagcttgc tggataagat ttctaaaggt gttaagaata
    17521 ggaagcgtgg cgaaccggtg aggctggttt acgacgaaaa gattgcccct gatttgctca
    17581 atttcatcgt gaagggttta tcattggacg agaaggacta cattttgccc ggtggtcgct
    17641 accacaactt taaggacttt atggattttc caaattttgg acgctccgac ctcgtcaatg
    17701 cccctatgaa accagtgcag gttgccgaac ttgaaaatgc cagcagcatt cttggagtaa
    17761 tggccaagca cgactttatc ctgcactacc ccttccatga ttttgcctac tacgttaaaa
    17821 tgctccgtga ggctgctatt gatcccaagg taactgctat ttatattacc ctataccgca
    17881 tggctgccga atcgcgggtg gttaacgctt tgatgaacgc tgcccaaaat ggcaagaagg
    17941 ttaccgctgt tatcgagctt cgcgcccgtt ttgacgaaca tgccaacatt tactgggcta
    18001 agcaaatgca ggaggccggc gttaacgtgg tgtttggaat gccgggcatt aaggttcact
    18061 ctaaaatgac cctgattatc cggcacgaag gtcgaaagga tgttaggtat gctgttgtta
    18121 gcacgggtaa ctttcatgag ggtaacgcca acctctacac cgacgttaac ctttttactg
    18181 taaaccaggg catcgccagg gaggttgaaa aggttttcac ctttctggaa tttaactaca
    18241 agacatttag ctacaaacac ctgcttgttt cacctgtaaa tatgcgtcgg aagctgtact
    18301 cccttatcga aaacgagatg gataatgctg ccaaaaaact accggcctac atccactgca
    18361 aaatcaacaa cctggttgat accgaaatga ttgccaagct ctaccaagct agtcaggccg
    18421 gggttgaaat taaactcgtg gttaggggga tttgctccct tgttccgggg gtaaaagggg
    18481 tgagcgataa tattgaggtt ttcagcattg ttgacaggct gctggagcat tcccgaattt
    18541 ttatttttgc taatagtgga aaggagacct gctacatttc atctgccgac tggatgacac
    18601 gtaatctcga ctaccgggtt gaaatagcaa cacctattta cgacaaacat ttaatttccg
    18661 agcttaaaac cgtaataaac atggcgttaa aggataatgt taagtcaagg gttatcgatc
    18721 agaaacaaag caactccttt agaaaccctg aggcgggaga agaaccattt cgctcacagc
    18781 ttgaacttta caggtactat aaatcaaaag agaatttaca aagtgctgcc acggaagatt
    18841 acaacttgac gtaagagtat atcgctcccc tggcatgtac tactgtattg aatttatgca
    18901 tttcttttat tcattaacag tttattttac cactggacag tttgcttcac taaatgaaac
    18961 tactcaaatt tgctgcaatt gatataggat cgaacgctgt tcgtttactc ctctcgaatg
    19021 ttgtggaaaa tggtgatagt gtagtattcc gcaagtcctc actcattcgt atgcccattc
    19081 ggttgggtga ggatgccttt gaaaccggta tcatctccga agcccgcatt cagaagctga
    19141 ttaaaaccat gattgcctat cgcaacctaa tggaggtgga ggatgttgtg gcttaccgtg
    19201 cttgtgcaac atcagccatg cgagaggcgc ttaatgcacc tgagattgtg gagcgtgtga
    19261 gtaggcaaac cggagtaaat attgaggtga taaatggtag gcaggaggct gagattgttt
    19321 actcaaatgg cattgccgaa acgttagata gttcaaaacc cttcatttat gttgatgttg
    19381 ggggtggcag caccgagatt tccctttttc aaaacggaca gaatattgcc tctcaatcat
    19441 ttgacatcgg aacaattcga atgctgaagg aaaaagtagg caaatcggag tttgacctta
    19501 ttaagaagtg gctaaaaaat ctgaaaatta ccggcggaaa gcctaaaatt ataggctcgg
    19561 gcggaaacat caacaagctc taccggctgg ctggaaaggt tgagggaagg ccgttgtcgt
    19621 ttgccgagct tcagaagcta ttcgcacagt taaaatccta cacctttgaa gagcgtatca
    19681 gcatcctggg aatgaacccc gatagggctg atgtgattat tcctgccgcc aggatttacc
    19741 tgaaaacgat ggagtggacc ggggccagta aaatactagt tcccaccatt ggcgtttcag
    19801 atggtattgt gcgaatgctt tacaagaatt accggagtag aagctagcgc aggttttcta
    19861 cttttctatc catacaaatt gtgcaagcgg tttactttta ggggcaattt gcacgctacg
    19921 agccaatcat caatctcgcc aatgattgct acctttgcaa aaaataaccg aataatggtg
    19981 gagcagcgca aacgggttgc cttttacacg ctaggatgta agcttaactt ttccgaaacg
    20041 tcaaccattg ccaggcagct tgtcgaaatg gggtacgaaa gagtttcgcc cgacgataag
    20101 gccgatgttt acgtgattaa tacctgctcg gttaccgagc atgccgataa gaaatgcagg
    20161 caagccatca ggaagtttgc cagtcgttcg cccggcgccc tgatagccgt agtgggttgc
    20221 tacgcccagc ttaaacccga tgaggttgcc gccattgaag gggttgacct ggtagtaggc
    20281 gcttcagcca aattccagct gccccagctg ctagctacac atgtaggtaa ggatggagca
    20341 aaagtttaca gctgcgatat tgacagggta gccgattttt ctccagctta ctcctcgggc
    20401 gatcgaaccc gttcgtttct taaggttcag gatggatgcg actaccattg cagctactgc
    20461 accataccgc ttgccagagg gaaaagcagg aatatgcctg ttaccaacat tgtggcacag
    20521 gctcaggcaa ttgcagcaca aaacgttaag gaaatagttc ttacaggggt taataccggc
    20581 gatttcggta ggtcaaccgg tgaaagtttc atagagctgc taaaagagct cgatgccgtt
    20641 gagggtattg aacgctacag gatctcctcc atcgagccaa accttataac cgatgaaata
    20701 gcccattttg tggccagctc aaaaaagttt ctgccccatt tccatattcc tcttcaatcg
    20761 gggtcgaaca gaatactcgg gctgatgcgc cgccgctaca accgggagct cttcgccgaa
    20821 aaaattgcca tgattcgccg gcttatgccc gatacttttt ttggggttga tgtgattgtg
    20881 ggatttcccg gcgaaaccga agccgacttc atggacggct acagcttcat acgggaggtt
    20941 aaccctgcat tcctgcacgt ttttccctac tccgaacggc ccaacacagc tgccgctctg
    21001 ctgccagcta aggttgacgc aagggaaatt aaggttcggg ctgaccgcct tggcgcgcta
    21061 tccagcgagc tgcaccggca attctacatt tcccgaaccg gccaggttca ccatgttttg
    21121 cttgaggcct cccaaaaatc ggggctaatg tttggcttca ccgaaaacta tatacgggta
    21181 gccgttccat ataaccgtaa tttggctgga aaagtggttg atgttcggtt agctgctcta
    21241 aatcctaatg ggttcatgga gggtgagctt atgcaaccat gaatcaacca tgtaggggtt
    21301 tgcacagacg cgtatgaaca accggacgat atttgcgagc ccggcgaagc caaaaatgag
    21361 tgttcagcgc gctcaatcca gatatctaaa aggtatgggc aaaatcagaa ttcaaaaaat
    21421 tttgtacgga tggaatggca tactattgct tggtgctagc taattggggc ataatccgaa
    21481 tttacccgaa acgctaccaa cccatttttt ttactaacat ttaccgaaaa gcgttatttt
    21541 cgtggattaa tttcctacag gcctcatcat ataaagatgt ggctgacagg gcgaagctgc
    21601 atagttggct atagctattt tttaaaattg cttcgttgtt ttggcttcca tccccgttta
    21661 attgtaagtc ctt
//
LOCUS       DAOSHM010000041        20804 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_2079_length_20804_cov_20.894646, whole genome shotgun
            sequence.
ACCESSION   DAOSHM010000041 DAOSHM010000000
VERSION     DAOSHM010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 20804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20804
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2079_length_20804_cov_20.894646"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(52..1029)
                     /locus_tag="PK839_07365"
     CDS             complement(52..1029)
                     /locus_tag="PK839_07365"
                     /inference="COORDINATES: protein motif:HMM:NF005741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HOG72589.1"
                     /translation="MANLTTSYLGLNLKSPLVVSSSNLTASVERIKNFEEMGAGAVVL
                     KSLFEEQIQHEIGNLAQYSDYTEAADYLKHYVTANNVNTYIELIRKAKSAVGIPVIAS
                     INCMSASQWVTFASQIQEAGADALEVNVFMLPVDPHKTSAEYESIYTSLAEKLTRIIS
                     IPIAFKVGPHFTNPFAVIEKLWYLKIRGVVLFNRFFEPDFDINTLKVIPASIFSHPDD
                     IRNSLRWVGMTNHLVPKIDISASTGVHDGEAMIKMILAGASSVQVCSAIYMNGAKHIA
                     SMLSKLSEWMDTMGFASINDFKGKLNYGQIHDPALYERSQFMKYYSSFE"
     gene            complement(1067..1738)
                     /locus_tag="PK839_07370"
     CDS             complement(1067..1738)
                     /locus_tag="PK839_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005779953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HOG72590.1"
                     /translation="MSIGTNLLETINSLPKGVTLVAVSKTHPPESIMEAYNAGQRVFG
                     ENKVQELVSKYEVLPRDIEWHLIGHLQSNKVKYVVPFVRLIHSVDSLRLLVTIDREAQ
                     KVNRVVDCLLQIHIASEETKFGLSADELEQLLDAEEYNLLQHIRVVGLMGMATFTENK
                     EQVRMEFRFLRQLFERIQEKYFVGKPWFCELSMGMSSDYRIAVEEGSTMVRIGSSIFG
                     QRVYR"
     gene            complement(2212..3453)
                     /locus_tag="PK839_07375"
     CDS             complement(2212..3453)
                     /locus_tag="PK839_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HOG72591.1"
                     /translation="MVKIKSFKGIRPPKELVKEIAARPYDVLNSAEAQKEAGEKSLLH
                     ITKPEIDFNPIIDEHSKEAYDKAVENFRKWKEKGWLVQDKKENYYIYAQTMEGRTQYG
                     LVACCHYEDYMEGRIKKHELTRPDKEEDRMIHIRLQNANIEPVFFAYPAQQEMDEIVN
                     GVVRSCKPEYDFVAADGFGHKFWVVDNDETIRRITGIFDRIPALYVADGHHRTAAAAR
                     VALEKKNANPNHRGDEEYNYFMAVIFPDNQLKIIDYNRVVKDLNGLTSEEFIQKIRKG
                     FDIVEVGTQTYKPQKLHNFSMYLDGKWYSLTAKPGTYNDNDPIGVLDVTILSNLILDQ
                     VLDIKDLRTSKRIDFVGGIRGLDELKRRVDSGEMKVAFALYPVSMKQLITIADTGNIM
                     PPKTTWFEPKLRSGLVIHSLD"
     gene            complement(3891..4814)
                     /locus_tag="PK839_07380"
     CDS             complement(3891..4814)
                     /locus_tag="PK839_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HOG72592.1"
                     /translation="MTKVLVATEKPFAKTAVEGIRKIVEDAGFELALLEKYTDPNDLV
                     KAVTDADALIVRSDKVTKEVVDAAKKLKIVVRAGAGYDNLDLAACSAKGIVAMNTPGQ
                     NSNAVAELAIGLMIYMARNLFQPGTGTEISGKKLGIHAYGNVGKLVAKYGKGFGMAVC
                     AFDPFVSKDEMEKDGVKAVDSIDTLYSKCDYVSLHIPANDKTKKSIGYELLSKMPKGA
                     TLINTARKEVIDEEGLKKALAERPDLKYATDIMPDCHAELVEKFPGRVFATPKKMGAE
                     TAEANVNAGLAAARQIVAFIKNGDKTFQVNK"
     gene            4813..4968
                     /locus_tag="PK839_07385"
     CDS             4813..4968
                     /locus_tag="PK839_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72593.1"
                     /translation="MIVKFLVNVVLEKFVILNKEKYPFPYQNKICTNRNTKSFGKIAQ
                     NDKKAIR"
     gene            complement(4978..6048)
                     /gene="serC"
                     /locus_tag="PK839_07390"
     CDS             complement(4978..6048)
                     /gene="serC"
                     /locus_tag="PK839_07390"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HOG72594.1"
                     /translation="MKKHNFNAGPCILPEVALQNTAKAVIDLDGIGMSVLTISHRSKE
                     FEAIMDETKALWRELLHIPDNYHILFLGGGASLQFCMVPYNLLQKKAVYLETGVWAKK
                     ALQEAKIFGDATALASSADKNFTYIPKGFKIPTDVDYFHVTTNNTIYGTELKEDIDSP
                     VTLVADMSSDIMSRPVDIKKYGLIYGGAQKNVGPAGVTFVIIREDILGKVDRKIPTML
                     DYRTHIKEDSMFNTPPCGAIFTMRETLKWVKSIGGVEKINELNKKKAQLLYDAIDSSK
                     MFVGTVEKDSRSIMNICFVMKPEYKDKEDAFMDFAKKAGMVGIKGHRSVGGFRASTYN
                     ALPIESVQALVDCMREFEKQNA"
     gene            complement(6179..6892)
                     /locus_tag="PK839_07395"
     CDS             complement(6179..6892)
                     /locus_tag="PK839_07395"
                     /inference="COORDINATES: protein motif:HMM:NF015962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HOG72595.1"
                     /translation="MNTIEVALSQSEFGCFDPKSKIVVVVDILRATSVICTMFKNGVS
                     EIIPVRSIDEAKTYKQKGFMVVAERNGQKLDFADFGNSPFYFTPDKVSGKTVVYSTTN
                     GTNAIMEGSKADTVIIASFLNFSSIFEFLKGGQSDVLILCAGWKGKFCLEDTIFAGAL
                     AYSLINSGNFTTICDSTNAAIDLWKVASPDMHSYLEKVAQRHRLKKLGLDDVIDYCFT
                     MDQTTAIPVLKGGSLIRLC"
     gene            complement(6942..8051)
                     /gene="gcvT"
                     /locus_tag="PK839_07400"
     CDS             complement(6942..8051)
                     /gene="gcvT"
                     /locus_tag="PK839_07400"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013547269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HOG72596.1"
                     /translation="MKNTPFTKYHIELGARMAPFAGYNMPIEYSGISDEHITVREKVG
                     VFDVSHMGEVWVKGPNALEFLQTVTSNDVSALTNGKVQYSCFPNDKGGIVDDLLVYRF
                     DEQTYFLVINAANIEKDWSWLCQQGQRFGLTPGKELYNASDEFAQLAIQGPFALKAMQ
                     KLTSANVTDMEYYTFKKIPFAGIREVIFSTTGYTGAGGCEIYCSPEDADKLWKAVFDA
                     GKEFGIKPIGLGARDTLRLEAGFCLYGNDLNDSTSAIEAGLGWITKFVPGKNFISREL
                     WEKQKNEGTERRLKGFVMIDRGVPRQHYPIFDAQGNPIGEVTSGTMSPLTKLGIGMGY
                     IKKGFWNDGSEIFIGIRDKKLKAKIVKPPFHQVKL"
     gene            8393..8761
                     /locus_tag="PK839_07405"
     CDS             8393..8761
                     /locus_tag="PK839_07405"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HOG72597.1"
                     /translation="MGMTSNEAAKHVNLEILKEISDGNTDLMVDLINLFIGQVRTFSD
                     QLELYNRKKDYECMAKLAHKIKNSVAMMGIDELSSDMKKLERLAQEGKKTNLYDNFIE
                     RFKKISGEAVIELKAILNSL"
     gene            8780..9115
                     /locus_tag="PK839_07410"
     CDS             8780..9115
                     /locus_tag="PK839_07410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOG72598.1"
                     /translation="MIEVEKINDAMVISFGSDNKLNVTVTQKIKTEIIKLIDPNSKII
                     LNLSGVSYIDSTGFGMLLSILRHCKNSNSQLKLCNISPEVMELIKLLQLQNIFDIREG
                     VNESLKNFC"
     gene            complement(9259..11766)
                     /locus_tag="PK839_07415"
     CDS             complement(9259..11766)
                     /locus_tag="PK839_07415"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOG72599.1"
                     /translation="MNCKFLVVLLLAVSLLGLRNLAAQTKNQPAGGRYDLSSFSGSIS
                     GQVLDSLTREGVEFASIAIFRQKDSSLVNGTVTDKNGSFKIDKLSPGQYYVDVKFIGY
                     NARRFQGIAVSPRTPEVNIGKIMIVQAYENLSDVVITGEKRMLQYNLDKKVINVEKDI
                     ASQGGTAIDVLQNVPSVNVDTEGNISLRGSENVNVLIDGRPSNLTSLDELPAQMIESV
                     EVITNPSARYDPDGLSGIINIVLKKKKDPGYNGMASIMLGTGGQYNGTLNFNWNRNKW
                     NIFANYDFRVMDRNFNSIAKRTSFLDSDGSMDSLSFLAQNTSGVRNGAFHSLRGGFDY
                     SLGPKATLSFTGNLNIRDFDSKNLTASNSSNSYSSYSSLEDRNNIGNSGGLGQMYSLN
                     FKKTYDAPGKEWTADLYFSRFGNDDKSNISNNAFINSVPEVSSERSNTDSWSNMLTFQ
                     TDYVRPVGNGGRLEAGVKSTIRKSDSDYQYDIFNTGLNTWDFDSTRSNHFIYDEQIYA
                     AYGIYSNSFGQGKFSYQLGVRVEDQIAKSDQQTTGEVADTNRLNFFPSAHIRWEPNSS
                     NSFQVSYSRRVNRPMTWILNPFLNTADKYNWSMGNPYLEPEFTTSLDFNYGLNFPSTK
                     VSASVYYRDTQNGFSRKLALVDSVTTLSTFINLSHNKSYGVEGVVTQNLTKWWRLNAS
                     YSYFKTKLYGDIVNGADEGTAWTAKLTSFFNIKKKIDIQVNGNYRSPILSASGTGNGF
                     HSIGGAQGKTKEMYWVDLGARISVLNNKGTITVRASDIFNTQKSRSNSWDTNFTSYSE
                     NWRNSQMLFVGFSYKINNYKARKVNDAEMDQTNDFME"
     gene            complement(12350..13645)
                     /locus_tag="PK839_07420"
     CDS             complement(12350..13645)
                     /locus_tag="PK839_07420"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HOG72600.1"
                     /translation="MEVLQVEKIEEQIARKYDFLENLEAWLANQGFSENFAQLLKVGI
                     SVGVIILLALLTDYIAKRIFLSTMQRVAKRTTSEWDDVLVEKKFFQHLAHFAPAAVVY
                     FTVGIALYDYTPKITLIIQALAKIYMVIVGVLVFNSFFDSVNTIYQKLPFAKSRPIKG
                     YLQVVKIILYVFAGIIVIGIIINRNPGTILVGLGASTAVLMLVFKDTIVGLVASIQLS
                     ANNMLKIGDWIEMPGRNIDGTVTDVSLTTIKVRNFDNTISTVPPYALVTESFRNWRGM
                     EESDGRRMIRSIFIDAKTIKFCSPQLLEKLKEVSYCHDWVVEQDEDELKRANEDIAML
                     NKGIPTNLYLYRRYLEGYIKSHPEVNQELPLVVRLKQQVEYGIPVEMYCFLKDKSWVP
                     YETTQSDIVDHALAALPVFELRTFQRMTSDDIRPTAGRS"
     gene            complement(13802..14173)
                     /locus_tag="PK839_07425"
     CDS             complement(13802..14173)
                     /locus_tag="PK839_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3127 domain-containing protein"
                     /protein_id="HOG72601.1"
                     /translation="MSMEITGKLLQKLNRQTGEGKNGAWVKQEFIVETLEQYPRKICI
                     AAWGTRVDDLDRLQPGNILKISVNVESREFNGRWYTDVKAWRIEKQDDEAPQSDGGKP
                     GKEDMNLPPESSKEYFDDLPF"
     gene            complement(14245..14961)
                     /locus_tag="PK839_07430"
     CDS             complement(14245..14961)
                     /locus_tag="PK839_07430"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOG72602.1"
                     /translation="MKLNKLLAAAILFPAVVSCQSTNGKIGKVAMKNDTDSISYAIGV
                     MYAQNLKQSQLETVNYQAVARAIQDIYSGDSANVKMSDNQAMAYINEYFRKLDQKIAE
                     KNLAESTKFLDENKTKEGVVVDSTGLQYKIITEGNGPIPTADAVVKVHYKGTLVNGKQ
                     FDSSYENGEPAQFKLNQVIRGWSIGLQKMKVGSKFMLYIPADLAYGVNVRPGGPIGPN
                     QALIFEVELLDIVKEEPAKK"
     gene            complement(15239..15916)
                     /gene="mnmD"
                     /locus_tag="PK839_07435"
     CDS             complement(15239..15916)
                     /gene="mnmD"
                     /locus_tag="PK839_07435"
                     /inference="COORDINATES: protein motif:HMM:NF033855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD"
                     /protein_id="HOG72603.1"
                     /translation="MTKLISAITSDGSSTIYSELHGAYYHSLNGALTESKHIFVEAGY
                     KEAIRVRGDVSILEVGFGTGLNAALTAAEASYSGIKTSYMAIEKYPVTDEVIKTLNYG
                     EMLSTEAATLWGKIIRAPWSCEVQISSLFSITKICTDFILWPAPPNRYNLIYFDAFAP
                     DDQPEMWTQPQFLKLFNSLLEGGILVTFSSKGSVKEALRQSGFTVHRIPGPPGKRHIL
                     RACKSAT"
     gene            16134..18248
                     /locus_tag="PK839_07440"
     CDS             16134..18248
                     /locus_tag="PK839_07440"
                     /inference="COORDINATES: protein motif:HMM:NF014896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HOG72604.1"
                     /translation="MAARLWNVKFYLLGLVLLNSCSLAPAPPKAEVHPYQMVTCGDTR
                     IDPYFWLCYRDSQKVLDYLLAENAYTEAMMKSTMPLQEKLFNELKSRKKVEDVTVPAF
                     MNGYFYYTRFEEGKEYPINCRRKGNMDAPEEVILDENEVAAGQRYCQVASLDISPNNQ
                     ILAFGVDTLGKRLFTIYFKNLETGKLLPIKIDNTTGTIAWASDSQTLFYTQKDQTLRP
                     SRIYRHKISKNANPKDELVYYEADSTFSTHVVNSKSLEYIFIFSSSGSCSEYRYLKTS
                     TPEGKFKVFQPRMPGVEYTVEHSGNFFYIMTSWNAENNRIMETPLTSTRKESWRELVP
                     NRSDVLVMNVDIFKDFLVATERKDALIQVKVISLRDKSQHYIDFGEEVYTALPSVNSE
                     FNTSSFVYFYTSLTTPGVTCEYNMQTHQKKILKQNEVLGGYNPDNYETKRLWATAADG
                     VNIPISVVYRKGIKLDGKNPLLIYGYGAYGYPVNPTFDPNLLSLLDRGFVYAIAHVRG
                     GQELGRKWYEDGRKLCKMNTFTDFIACTECLVEQKYTSPDRIVAQGASAGGLLMGVIA
                     NLRPDLYRAIVAEVPFVDVLTTMLNPNLPLTTGEYDEWGNPNDSIVYHYIKSYSPYDG
                     VRQQDYPSMLVLASYYDSQVQYFEPAKWVAKLRASKTDNNLLLLKTDMESGHAGSSGR
                     YKTLKDEAFVYAFILHAVGIED"
     gene            complement(18272..20629)
                     /locus_tag="PK839_07445"
     CDS             complement(18272..20629)
                     /locus_tag="PK839_07445"
                     /inference="COORDINATES: protein motif:HMM:NF020037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOG72605.1"
                     /translation="MEKPDNTDSSGIEDKYQALLGLIPDMIFIFDSDVRYVGYHGGKD
                     APIYVPPEMFLGKRIEEVLPEPVASNARVVVEEVLKTGKMQTYSYQLTIDNKPQFFES
                     RMVRYGTSKVLAIVRDVTSRTNAENALKESEEKYYNLYNLMRLMADTIPDMVWAKDLN
                     QNYIFANKAFCSTILNARDTDEPVGKNDMFFAKRERESHPENPEWHTFGELCMNSDLD
                     TLRELKEMQFDEYGNVKGKFIFLDVHKSPLYDKNGKLIGVVGSGRDITEKKKMEEHLS
                     LSEKTYRGIINSISEAVYVQDADGTFLDVNKACEKYYGYPREYFIGKKPKDLSAPEIN
                     DLDKVTDYVSKAFDGQPQIFEFWGLRNDGTIFPKEVSLTPGEYFGRKVVIAVSRDITE
                     QKNWEAELIAAREKAEESDRLKSAFLANMSHEIRTPMNGILGFAQLLKNPRLSEEEVR
                     EYLDVIEQSGQRMLDIINNLINISKIESGEVQVNLSQCDVNEQLQYALNFFKPDADSK
                     GLKFFLEYQLPDEQATVTTDPLKLQVILSNLVKNAIKYCDNGSVAFGCKLKEEYLEFY
                     VSDTGIGIPPMTIGKIFERFVQANTNYSGRYEGAGLGLTIAKSYVELLGGSIWVESEV
                     GKGSTFHFTIPVRPAARKQQSYSNALNQDKNDSVSQPFSKKLKILIAEDDVPSGILLK
                     NQLRDIAGEIISARNGADAVSMFLSNPDTDLILMDIKMPEMDGYEATKKIREVSSNVV
                     IIAETAYAMTNDREKALSVGCNDYISKPINREKLLELVKKHFELD"
BASE COUNT         5620 a         4761 c         4399 g         6024 t
ORIGIN      
        1 cactctaaca aactttctta cctaatacaa cattccgttt taagggaagg cctactcgaa
       61 gctggagtag tacttcatga actggctacg ctcgtagagt gcaggatcat gaatttgacc
      121 gtagttcaat ttccctttaa aatcgttgat gctggcaaat cccatagtat ccatccattc
      181 ggacagctta ctaagcattg aggcaatatg ctttgctcca ttcatgtaga tagccgagca
      241 aacctgaacc gacgaggcgc cagccagaat catttttatc attgcctcac cgtcgtgaac
      301 tcccgtagaa gccgagatgt caatctttgg caccaggtgg tttgtcattc ccacccaccg
      361 cagcgagttc cgaatatcgt cgggatggct gaaaatagat gcgggtataa cctttagggt
      421 gtttatgtcg aaatcgggtt cgaaaaaccg gttaaaaagc accactcccc taatcttgag
      481 gtaccaaagc ttttcaatca ccgcaaaagg gttggtaaaa tgtggtccca ccttaaaagc
      541 tatgggtatg gaaattatcc gggtaagctt ctcggccagg cttgtgtaga ttgactcgta
      601 ttcagcagag gttttatggg ggtcaactgg tagcatgaac acgtttacct ccagcgcatc
      661 ggcacccgcc tcctgtatct gcgaggcaaa agtaacccac tgcgaagcgc tcatgcagtt
      721 gatgctggca ataactggaa taccgacagc tgattttgcc ttgcgtataa gctcaatgta
      781 ggtgttcacg ttgttggcag taacgtaatg cttcaggtag tcggctgctt cggtatagtc
      841 ggagtactgc gccaggttgc caatctcgtg ttgaatttgc tcttcgaaca gcgacttcag
      901 cacaacggcg cctgcaccca tctcctcaaa atttttaatc cgctcaaccg aggcggtaag
      961 gttggagctg ctcaccacca gcggcgattt taggtttaac cctaggtatg aggttgtaag
     1021 gtttgccata gctgtatttt ttagttgatt tatggttacg atgaacttac ctgtaaactc
     1081 gctggccaaa aatgctgctg ccaattctca ccatggtgct tccctcctca acagcaatcc
     1141 tgtagtcgga ggacatgccc atggagagtt cacaaaacca aggtttcccg acaaagtatt
     1201 tttcctgtat tctttcgaaa agttgcctga ggaacctgaa ttccattctc acctgttcct
     1261 tgttttcagt aaaagtagcc attcccatca atccaacaac ccgaatatgc tgcagcagat
     1321 tatactcctc agcatccagg agctgttcca gctcatcggc cgacagccca aacttagttt
     1381 cctccgaggc tatgtggatt tgcaacaaac aatccacaac tcggttaacc ttttgagctt
     1441 ctctatcaat ggtaaccagt agccttaaac tatccaccga gtggattagc cgtacaaagg
     1501 gaaccacgta ctttacctta ttcgattgaa gatggcctat tagatgccac tcaatatctc
     1561 ttggcaacac ttcgtacttc gataccagct cctgcacctt attctccccg aaaacccttt
     1621 ggcctgcatt atatgcctcc atgatgcttt cgggaggatg ggtttttgaa acggcaacca
     1681 gcgtaacccc ttttggcaac gagtttatag tttcaagaag atttgtccca atactcatta
     1741 cagtaaggtt tgatgcaaat atagaatatt ttgcctatgg gcaaaaggaa aaattttgcc
     1801 gctcatcgct cttaagtcct aaccttaact ccttgcaagt cgattttttt ctcaaactct
     1861 gtcaattctc tgataaagtg atgtttatcg ttatgatacc aaccagcgtt tttcctgtta
     1921 attcaacctt tttctattga ttgctggcat atcataagat tctccttgcc accgataaca
     1981 tcctgcaagt aatattccga ctaaattgca aaccgctgtg tagcttggcc ccgctttgtt
     2041 gctacttgag catcccaaca gctaccctag cataacaggt tgtagtgatt tttttttagc
     2101 tgattcaaca gcgtagtaaa atttatgtgt ttatttacgc ttaagtaaga atttctctta
     2161 gtgtagtgcc attttatagc aaataaatgg gctgacttgc agcccattta tctaatctaa
     2221 ggagtgaata accaacccgc tgcgaagctt aggttcgaac catgtagttt tgggtggcat
     2281 gatgttgccg gtgtcggcaa tggtaatcag ctgcttcatc gaaacggggt aaagggcaaa
     2341 ggccaccttc atttcgccac tgtcaacacg cctttttagc tcgtccagcc cgcggatgcc
     2401 gccaacaaag tctatccgtt tcgatgttcg caggtccttg atgtcgagca cctgatcgag
     2461 gatgaggttg gagagaatgg taacatccag cacgccaatg gggtcgttgt cgttgtatgt
     2521 acccggtttg gcagttaggg aataccactt accatcaagg tacatgctga agttgtgcag
     2581 cttctgcggt ttataggttt gggtgccaac ctcaacaata tcaaaccctt tcctgatctt
     2641 ttgaataaac tcctcggagg ttaggccatt caaatcctta acaacacggt tgtaatcaat
     2701 tattttcagc tggttatccg gaaagattac cgccataaaa tagttgtact cctcatcgcc
     2761 ccgatggttt gggttggcat tctttttttc gagagcaaca cgggcggctg cagccgttcg
     2821 atgatggccg tcagccacgt aaagagcagg aattcggtca aatataccgg ttatgcgccg
     2881 aattgtttca tcattatcaa ccacccagaa cttgtgtcca aatccatcag cggctacaaa
     2941 atcgtactcg ggtttgcagc ttctaaccac gccattgaca atctcgtcca tttcctgctg
     3001 agcggggtag gcaaagaata ccggttcaat attggcattc tgcagcctga tatggatcat
     3061 cctgtcctcc tccttatcgg gacgggttag ctcgtgcttc ttaatcctac cctccatgta
     3121 gtcctcataa tggcagcatg caaccaatcc gtactgcgta cgaccctcca tggtttgagc
     3181 atagatgtag tagttctcct tcttatcttg aaccagccag cccttttcct tccacttcct
     3241 gaaattctca accgctttgt cgtaggcttc cttggaatgc tcatcaataa ttgggttgaa
     3301 gtctatttcg ggcttggtaa tgtgaagcag cgatttctca ccggcttcct tctgagcttc
     3361 agccgagtta agcacatcat acgggcgagc ggcaatttcc ttaaccagct ccttaggggg
     3421 ccgaatgccc ttgaatgatt ttatttttac catgaaaagt taattgttaa gagttgaaaa
     3481 gttaaaagac aataatgtta aacggttgat tttaaggctt ttaatcacgc caaaatttaa
     3541 ttgcttaact aaaagaatat gcttcataat ttctgatata ttcgatatat accctagcct
     3601 attctaagtt attagctgtg tttctaattc ggctaaagat tcagctgcaa aataaagaaa
     3661 atgaatttgt ttctgccgaa ctatttcttg cacagcccac agcaatattt gatggaatag
     3721 aaactactgc tcgactaatc tgagaggtta acccctaagt ctcttcttta ggaaaagatt
     3781 tagttagttc ataaatttca actgttaact ctacagcctc tttccaagcc tctagcttag
     3841 tatgctccac cttctcgttt aacttttcac ttttaacttt tcacctgtta ttacttattc
     3901 acctgaaaag ttttatcgcc attcttgata aaggcaacta tttgacgggc agcagccagg
     3961 ccagcgttaa cgttggcttc agcagtttcg gcacccattt tcttgggtgt tgcaaacacc
     4021 cttccgggga atttttccac cagctcggca tggcaatcgg gcataatgtc ggtggcatat
     4081 ttcaaatcgg gacgttcggc caaggctttt ttcaacccct cctcatcaat aacctccttg
     4141 cgggcagtgt tgattagtgt agcgcccttg ggcatcttgg agagtaactc gtacccgata
     4201 gacttctttg ttttgtcgtt tgcaggtatg tgcagcgaaa cgtagtcgca cttcgagtaa
     4261 agtgtatcaa ttgagtcaac agcctttacc ccatccttct ccatttcatc cttggataca
     4321 aacgggtcga aggcgcaaac agccattcca aatcccttgc cgtacttagc caccagctta
     4381 cccacgttgc cgtaggcatg aataccaagt tttttgcctg aaatctcggt gcctgttcct
     4441 ggttggaaaa ggttacgagc catgtatatc ataaggccaa tggcaagctc ggcaactgcg
     4501 ttggagtttt gcccaggtgt attcatggct acaattccct tggctgagca agcagccaaa
     4561 tcgagattgt cgtagcccgc acctgcccgt accacaatct tgagcttttt agcggcatca
     4621 accacctctt tggtaacctt atcgctacga actataaggg catcggcatc ggtaacggcc
     4681 ttaaccagat cgtttggatc ggtatatttt tcaagaagag ccaactcaaa accagcatcc
     4741 tcaacaattt ttctaattcc ttcaaccgca gtctttgcaa aaggcttttc ggttgcaact
     4801 agtacttttg tcatgatagt aaagtttttg gttaacgttg tgttagaaaa atttgtcatt
     4861 ctgaacaagg agaagtaccc cttcccctac caaaacaaga tttgcaccaa taggaataca
     4921 aagagtttcg gcaaaattgc tcagaatgac aaaaaggcca tacgataaag gagtaagcta
     4981 agcgttttgc ttttcaaatt ccctcataca gtcaacgaga gcctgaacgc tctcaattgg
     5041 gagtgcgtta taagttgatg ccctaaatcc gccaaccgaa cggtgtccct taattccaac
     5101 cataccggct tttttggcaa aatccataaa agcatcttcc ttatccttgt actcgggttt
     5161 catcacaaag catatgttca tgatagagcg tgaatccttt tcaacggtgc ccacaaacat
     5221 cttgctgctg tcaatggcat cgtagagcag ctgggctttt ttcttgttta gctcattaat
     5281 tttctcaacg ccgccaatgc tcttgaccca cttcaacgtt tcgcgcatgg tgaagatagc
     5341 cccacaggga ggggtgttga acatggaatc ctccttgatg tgggttcggt agtcgagcat
     5401 ggttggaatc tttcggtcaa ccttaccgag aatatcctcc cgaataataa cgaacgtaac
     5461 tccggcaggc cctacgttct tctgggctcc accgtagatt aacccgtatt tctttatatc
     5521 aacagggcgg ctcataatgt cggagctcat atcggcaacc agggtaacag gtgaatcgat
     5581 atcctccttt agctcagttc cataaatggt gttgttggtg gttacgtgga agtagtcaac
     5641 atcggtaggg attttaaagc ccttggggat gtaggtaaaa ttcttatcgg cagaagaggc
     5701 tagtgcagtg gcatcgccaa aaatttttgc ctcctgcagg gctttctttg cccacactcc
     5761 ggtttcgagg taaacagcct ttttttgaag caggttgtaa ggaaccatgc agaactgcag
     5821 gctggcaccc ccaccaagga ataggatatg gtagttgtcg gggatatgaa gcaactcccg
     5881 ccacaaggct ttagtttcat ccataattgc ttcaaattcc tttgaacgat gggaaattgt
     5941 cagaaccgac atccctattc catcaagatc aatcacagct ttagctgtgt tttgaagtgc
     6001 aacttcgggc aggatacaag gcccggcatt aaaattatgt ttcttcatgg catttaggat
     6061 taaattttaa cagatttcct caaagatacg tgaaatttta aagttaaacc caaacatttc
     6121 aatgtaaatg ttataaaaca tttaccagtg aattccattt gcagggcagg taggctaatc
     6181 agcaaagcct aataagactg ccacctttta gcactggaat tgctgttgtt tggtccatag
     6241 taaagcagta gtctataaca tcgtccaaac ccagcttctt aagccggtgc ctttgggcta
     6301 ccttttcgag gtaggaatgc atatcggggc tggctacctt ccaaagatca atagcggcgt
     6361 tggtggagtc gcaaatggtg gtgaagtttc ctgaattgat cagggagtaa gctagggcac
     6421 cggcaaaaat agtatcctca aggcagaatt ttcccttcca accggcgcaa agtataagaa
     6481 catcagactg cccccctttt aaaaattcaa agatggagga gaagtttaag aacgaggcaa
     6541 taataaccgt gtcagcctta ctaccctcca tgatggcatt ggtgccgttg gtggtgctgt
     6601 aaaccactgt tttcccggac actttatcgg gagtaaagta gaaaggcgaa ttgccgaaat
     6661 cggcaaaatc gagcttctgg ccatttcgct cggcaaccac cataaagcct ttctgcttgt
     6721 aggtcttggc ctcgtcaatg cttcttaccg ggataatttc actgacgcca tttttaaaca
     6781 tggtgcagat tacagaggta gccctaagga tatcaaccac cacaacaatc ttactcttcg
     6841 ggtcgaagca tccgaattca gattgggaaa gggcaacttc aatggtgttc actttacact
     6901 tggtttaaaa gttaaggggg caaggccccc tttttggtag gttacaattt tacctggtga
     6961 aatggcggct taacgatttt tgccttcaac ttcttgtcgc gaattccaat gaagatttcg
     7021 ctgccatcgt tccaaaatcc ctttttgatg tagcccatgc caatacccag cttggttagg
     7081 ggcgacatgg ttcctgaggt aacctcgcca atcggattac cctgagcatc aaaaataggg
     7141 tagtgctggc ggggtacacc cctgtcaatc attacaaatc ctttaagcct gcgttcggta
     7201 ccctcatttt tctgtttttc ccaaagttca cggctaatga agttcttacc tggaacaaac
     7261 ttggtaatcc agcccaatcc ggcttcaata gccgacgtgg aatcgttaag gtcgtttccg
     7321 tagaggcaaa atccagcctc cagcctaagg gtatcgcgtg caccgagccc aatgggctta
     7381 ataccaaact ctttccctgc atcaaaaacc gctttccaga gcttgtcggc atcttcaggg
     7441 ctacagtata tttcgcaacc cccggcaccg gtatagccag tggtggagaa tattacctcc
     7501 cggataccgg caaagggaat ttttttaaag gtgtagtact ccatgtcggt aacatttgcg
     7561 gaagttagct tctgcatggc cttaagggca aacggtccct gaatggccag ctgggcaaac
     7621 tcatcggagg cgttgtagag ctccttacct ggagtcaggc caaacctttg cccctgctgg
     7681 catagccaac tccaatcttt ttctatgttt gcagcgttaa taaccaaaaa gtacgtttgc
     7741 tcatcaaacc tgtaaaccag aaggtcgtca acaattcccc ccttatcatt agggaagcag
     7801 ctatactgca cctttccgtt ggttaacgct gaaacatcat tgctggtaac ggtttgtaga
     7861 aattcaaggg catttggccc ctttacccaa acctcaccca tgtgggaaac gtcaaatacg
     7921 cccacctttt cccgaacagt tatatgttcg tcgctgattc cggaatattc gataggcatg
     7981 ttgtagcctg caaaaggcgc catacgtgct cccaactcaa tgtgatactt ggtgaatgga
     8041 gtgtttttca tctgcttact gtttaactgg tggttttatg atatagtaac gtattgctcg
     8101 catttcaaca taaaccgtta ttttaataga aaacatcggc cacctgctgg atgtgagccg
     8161 gagtggcatt ttattggttg gctaacaccc gtatttatca tgtacactac tacacctgca
     8221 aagctacttt ttttaaatgt cttattaaaa ttaaaagtta ccaactgcag taagatatgt
     8281 tgtaaagcag aaaagcagca atactagcta gctaagctca atttttttgc ataggataac
     8341 atttttttgt attgattaat ttttttgtta ctttagggta ctaaatggcg gtatgggaat
     8401 gacatcaaat gaagcggcaa agcatgtaaa tctggagatc ttaaaggaga tttccgatgg
     8461 aaatactgat ttaatggtcg atttaatcaa cctatttatt ggtcaagtaa gaacatttag
     8521 cgatcaactt gaactctaca acaggaagaa ggattatgag tgtatggcga agttggcgca
     8581 taaaattaag aactctgtag ccatgatggg tattgacgag ctttcctccg acatgaaaaa
     8641 actggaacgc ctggctcagg agggcaagaa aacaaacttg tatgataatt ttattgaaag
     8701 atttaagaaa atatcaggcg aggcagtgat tgagctcaag gcaatcctca atagtcttta
     8761 accctaaaac cttacaatca tgatcgaggt tgagaaaatt aacgatgcaa tggttatttc
     8821 ctttgggagt gataataagc tcaacgttac cgtcacccaa aagattaaaa ccgagattat
     8881 caagctgatt gatccaaact ccaaaattat tttaaaccta agcggcgtga gctacattga
     8941 cagtactggt tttggcatgc tactctccat tctcagacac tgcaagaatt ccaactccca
     9001 gctaaagctc tgcaacatat cgcccgaggt aatggagctt ataaagctac tgcagctcca
     9061 aaacattttt gacatccgcg agggggttaa cgaaagcctt aaaaacttct gctagctaat
     9121 accccctgtt aatttgtaat atacctttgg agcaatatgc tttatatcgc ttgtaaataa
     9181 tagcttctcg ttaaataaac ctgcattata caaaaggcga agcagtgttg cttcgccctt
     9241 tgtatggcgg ggtaaaccct actccatgaa gtcgtttgtt tggtccattt cagcgtcgtt
     9301 aacctttcgg gctttatagt tattaatctt ataggagaac ccgacaaaca gcatctggct
     9361 atttctccaa ttttcgctgt aggaggtaaa gttggtgtcc caggagtttg atctagattt
     9421 ctgggtgtta aaaatatcgc tagccctaac agtaatggta cctttgttat taagtaccga
     9481 tattctggca cccaaatcaa cccagtacat ttccttggtt tttccctgcg ccccaccgat
     9541 tgaatgaaac ccgttaccgg ttccactagc cgagagaatt ggtgacctgt agttaccgtt
     9601 tacctgaatg tcaatttttt tcttaatgtt gaaaaatgag gtgagcttag ctgtccatgc
     9661 cgttccctca tcggcgccat taacaatatc gccataaagc ttagtcttaa agtagctata
     9721 gctggcattc agccgccacc atttagtcag gttctgagta accaccccct caaccccgta
     9781 acttttatta tgtgacaggt taataaaagt agaaagggtg gtaaccgagt caaccaaggc
     9841 cagcttccgg gaaaatccgt tttgggtgtc gcggtagtaa accgaagcgc ttaccttggt
     9901 tgaaggaaag ttaagtccgt agttaaaatc gagcgatgtg gtgaactcgg gttcaaggta
     9961 ggggttaccc attgaccagt tgtacttatc cgcagtgtta aggaacgggt tgagtatcca
    10021 tgtcattggc cggttaaccc ttcggctgta cgacacctga aagctgttgc tggaattagg
    10081 ctcccaccta atatgggcgc tagggaaaaa gtttaaccgg ttggtatcag ccacctcgcc
    10141 tgtagtttgc tggtcggact tggcaatttg atcctccacg cgaacaccaa gctggtagct
    10201 gaatttgccc tggccaaagc tgttggagta aatgccgtag gctgcatata tctgctcatc
    10261 gtaaataaaa tggttggaac gtgtagaatc aaaatcccag gtatttaacc ctgtattgaa
    10321 aatatcgtac tggtaatcgg aatcgctttt ccgaatggtg ctctttactc cagcctccag
    10381 cctgccgcca tttccaacag ggcgaacata gtcggtttgg aaggttagca tatttgacca
    10441 gctgtcggtg tttgaccttt cgctcgaaac ttcaggtacg ctgtttataa aggcgttgtt
    10501 gctgatgttg cttttgtcgt cgttaccaaa cctggaaaaa tataagtcag cagtccattc
    10561 tttaccaggc gcatcatacg ttttcttgaa gttgagggag tacatttgcc ctaaaccacc
    10621 tgagtttcca atgttattcc tgtcctccaa actagagtat gagctgtagc tattgctact
    10681 atttgaggca gtgaggtttt tgctgtcaaa atccctgata ttgaggttcc ctgtgaacga
    10741 aagggtggct ttcggaccca aggagtagtc gaatccaccc ctaagactat gaaatgctcc
    10801 attccttact ccactagtat tttgagcaag aaaagaaagc gaatccatgc tcccgtcgct
    10861 gtcaagaaag gatgttcgtt tggcaatgga gttaaaattt ctgtccatta ccctgaaatc
    10921 gtaatttgca aaaatgttcc acttgttcct gttccagtta aagttcaagg ttccattata
    10981 ctgtccacca gtacctagca ttatgcttgc cataccgttg taccccggat ccttcttttt
    11041 cttgagcacg atgttaatta tacccgagag cccatcgggg tcgtatcggg cagatgggtt
    11101 ggtaattacc tcaacgctct caatcatctg agccggcagt tcatcgaggc tggttaggtt
    11161 agaagggcga ccatcaataa gaacgttaac attttcgcta ccccgtaggc tgatatttcc
    11221 ttctgtatcc acattaaccg atggaacatt ttgtaaaaca tcaatggcag ttcccccctg
    11281 gctggcaata tccttttcaa cattaattac cttcttatca aggttatact gaagcatacg
    11341 cttttcgcct gtaatcacta catcgctaag gttttcgtat gcctgcacta tcataatttt
    11401 cccaatgtta acctcaggag tacgtgggct aacagctata ccctgaaatc tgcgggcatt
    11461 gtacccgata aattttacat ccacatagta ctgacccgga ctcagcttgt caatcttaaa
    11521 actcccgttt ttatcggtta cagttccgtt caccaacgaa gagtcctttt ggcgaaatat
    11581 ggcaatactg gcaaattcaa ccccttcacg ggttagagaa tcaagcacct gcccgcttat
    11641 tgatcctgaa aagctactca aatcatatcg gccacctgcc ggttgatttt ttgtttgagc
    11701 agctaaattt cgcaaaccca acaatgagac tgcgagtaga agaactacta aaaatttaca
    11761 attcatgtgt taattaattt agtaaaatat gaaacatttg caactcattt gagcatatcc
    11821 cctctcagga gtttagcatc gccaggcaat tttaatatag tttcacattt tgaaatttgc
    11881 cgggtgttga taattgccct ttgttttaat atgctccaac aaaacattaa tttgacacca
    11941 ttgtttggtt aaacttgcgg tgtagtaaaa tattctttaa aacagggtta gatgctacgg
    12001 ctggtaatag ctacaatgga gagtggcgtt ggtttcatac agtataagcg ccaatgtagg
    12061 acagctatca caaaaacgga taaacaaccg gatgttattt gtgggttcgg cgaaatcttc
    12121 ctatgagtgt cagcctacgg caagcctttt ggggttcatc cgaatgtaag aggtagaaaa
    12181 atactaaatc gctgcttact gtcatcaaaa tataatattt gcttgtagaa aaagcgcagc
    12241 aatctacctc tggtattgca gctatagcaa tcaaacgggt aaggctgata aggagaggtt
    12301 caaaatttgc tcctataaca ggagtagaaa agcttagcta aacccttgat tagctcctac
    12361 cagcagtagg gcggatgtca tcgctcgtca tgcgctgaaa agttcggagc tcaaaaacag
    12421 gtagtgctgc cagggcgtgg tcaacaatat cggactgggt agtttcgtag ggaacccaac
    12481 tcttatcctt cagaaaacag tacatctcaa cagggatacc gtactccacc tgttgcttta
    12541 ggcgaacaac cagtggtagc tcttggttga cttcgggatg tgattttatg taaccctcca
    12601 agtacctacg ataaaggtac aggtttgttg gtataccttt atttagcatg gcaatatctt
    12661 cattagctct tttaagctca tcttcatcct gctcaacaac ccaatcatga cagtagctaa
    12721 cttctttaag cttttcaagc agctggggag aacaaaactt aatggttttg gcatcaataa
    12781 aaatggaacg aatcatcctt cgaccatccg attcctccat acccctccag tttcggaaac
    12841 tctcggtaac aagcgcatag ggtggaacag ttgaaatggt gttgtcaaag tttcttacct
    12901 ttatggtggt tagactaacg tctgtaactg ttccatcaat gttgcggcct ggcatttcaa
    12961 tccaatcacc aattttaagc atgttgttgg ccgaaagttg tatggaagct acaagcccaa
    13021 ctatagtatc cttaaacacc agcatcagca cagccgttga ggcacctaat cctaccagaa
    13081 tagttccggg gttgcggttt atgatgatac ctataacgat tattccggca aaaacgtata
    13141 gaataatttt cacaacctgt aggtagccct taatggggcg acttttagca aaaggcaact
    13201 tctggtatat ggtgtttacc gaatcaaaaa atgagttaaa aacaagaacg ccaactataa
    13261 ccatgtaaat tttagctagc gcttgaataa tcagggtgat ttttggagtg taatcgtaga
    13321 gggcaattcc aacggtaaag taaactacag cagccggagc aaaatgtgcc aggtgctgga
    13381 aaaacttttt ctcaaccagc acgtcgtccc actcgctggt ggtgcgcttg gcaacccgct
    13441 gcatggtgga caaaaagata cgcttggcta tgtagtcggt gagtagagcc aatagaatga
    13501 ttaccccaac cgaaatgcca acttttagca gctgggcaaa gttttcacta aatccttggt
    13561 ttgcaagcca tgcctccagg ttttcgagaa aatcgtactt tcgggcaatt tgctcctcaa
    13621 ttttttctac ttgtaacact tccacctagt aagttgattt aatggtttac tacttgcttg
    13681 ttgtgtagct ggttataaga tatttttaaa tgaagatgtt ggttaggcta tagaaatcta
    13741 aggcaatgct ttattcgcac gttatgaact tcagctgcaa gccaaaccag catgcagctt
    13801 gctaaaaagg caaatcgtcg aaatattctt ttgatgattc gggaggtagg ttcatgtcct
    13861 ctttaccggg ttttccccca tccgattgag gtgcttcatc gtcctgtttt tctatgcgcc
    13921 aagccttcac atcggtgtac catcgcccgt taaactccct cgactcaaca ttaacagaaa
    13981 ttttaaggat gtttccaggc tgtagccggt caagatcatc aacccgggtt ccccatgcgg
    14041 ctatacaaat ctttcggggg tactgctcga gggtttcaac aatgaactcc tgctttaccc
    14101 aagctccatt tttaccttca ccggtttgcc ggttcagctt ttgaaggagt tttcctgtta
    14161 tctccatgct catgttactt aaaatttaga aaaacagccg gatgaaccac acccggctgg
    14221 taagctgcga ggtttaacaa tatactattt tttagcgggt tcttccttaa caatgtcaag
    14281 gagctcaact tcaaaaataa gcgcctggtt gggtccaata ggtccaccgg gacgaacatt
    14341 aacaccatag gccaaatcgg ctgggatgta gagcataaac ttggaaccca ccttcatttt
    14401 ctgaaggcct atgctccagc ctctaattac ctggttgagt ttgaactggg cgggttcacc
    14461 attttcgtat gatgaatcaa actgctttcc gttaacaaga gtgcccttgt agtgaacttt
    14521 aaccacggca tcggcagtag gaatggggcc attcccctcg gtaataattt tatactgaag
    14581 tccggtggaa tcaacaacta cgccctcttt tgttttgttt tcatctagaa atttggtgct
    14641 ttcagccagg tttttctcgg caattttctg gtcaagcttc ctgaagtact cgttaatgta
    14701 tgccatagct tgattgtcgc tcatcttgac attggctgag tcgccactgt aaatatcctg
    14761 aatggcgcgt gccacagctt gatagttgac ggtttcgagc tgactttgct ttaaattctg
    14821 cgcatacatc acaccaatag catagctaat actatcggta tcattcttca tggccacctt
    14881 tccaatctta ccatttgtac tttggcacga aacaacggct ggaaaaagaa ttgccgcagc
    14941 aagtaacttg tttaatttca tctgattaat tttatttgat tagttgatat tcagattgac
    15001 acaaaattta tctcatccag ataacttcta gcaaactttc acaaccgcct cgggttcaac
    15061 agcaacgctt ctcttccact aatcctagaa ttgcctaaat acccaagttt tttttttaaa
    15121 attactcaac aataatattc cctgcgaagg taatacatat ttaaactgaa ttaaaatttt
    15181 agggtaaaaa tttcttggtg ctggtttttg aaacgaaaaa ttgtaacatt taacaatact
    15241 acgtggcaga tttacaagcc ctgagtatat gccttttacc gggaggtccg ggaatacgat
    15301 gaactgtaaa gcccgattgc ctcagggcct ccttcacgct gccctttgat gaaaaagtaa
    15361 ccagtattcc accttctagc aaggagttaa agagtttaag aaactgtggt tgagtccaca
    15421 tttcgggttg gtcgtcgggg gcaaacgcat cgaagtatat caggttgtac cggtttggag
    15481 gtgcaggcca taaaataaag tcggtgcaaa ttttagtgat ggagaacagg gagctaatct
    15541 gaacctcaca gctccaaggc gcccggatta tttttcccca gagggtagct gcctcggtgc
    15601 taagcatctc gccgtagttt agggttttta taacctcgtc tgttaccgga tacttttcaa
    15661 tagccatgta gctggttttg atgcccgaat agctcgcttc agctgcagtg agggcagcgt
    15721 taagcccggt tccaaacccg acctcgagta tggaaacatc acctcgtacc ctaatagcct
    15781 ccttgtaacc tgcctcaaca aaaatatgct tcgattccgt aagggcgccg ttaagtgaat
    15841 ggtagtatgc gccatgaagt tcggagtaga tcgtgctcga gccatccgaa gtaatggctg
    15901 aaataagctt tgtcatggat ttcaacacaa tagatgctca aaattagggg aattttaaat
    15961 attgaggtaa gtgcatataa ggagaagtaa ccagtaggag ctgaccatct gaatggatag
    16021 ttttccttac aatatatgca acgtattgta cgtgaaaatg ttacgaataa atgtggagta
    16081 gatttgcagg taaccggcca aaatcataat tttggtttta tttatcgaaa attatggcag
    16141 ctcgcttatg gaacgtaaag ttctacctgt tgggattggt tttactgaat agttgctccc
    16201 tggcacccgc tccacccaaa gctgaagtgc atccctacca aatggttact tgtggcgata
    16261 cccgtattga tccctacttc tggttgtgct atcgcgattc gcaaaaggtg ctggactacc
    16321 tgctagctga aaatgcctac acggaggcca tgatgaagtc caccatgcca cttcaggaaa
    16381 aactattcaa cgagttgaaa tcgcgcaaaa aggtagagga tgttacagtt cctgcgttta
    16441 tgaacgggta tttttactac acgcgatttg aggaagggaa agagtacccc attaactgcc
    16501 gcagaaaagg taacatggat gcccccgagg aggtaattct cgacgagaat gaagttgctg
    16561 ccggtcagcg gtactgccag gttgccagct tggatatttc gcccaacaac caaattcttg
    16621 ccttcggggt tgatacccta ggcaaaagat tgttcaccat ttactttaaa aacttggaaa
    16681 ccggcaagtt gctcccaatc aagattgata atactaccgg aacaattgcc tgggcaagcg
    16741 atagccaaac gctcttttac acgcagaaag accaaaccct ccgcccgtca aggatttatc
    16801 gccataaaat ttccaaaaat gccaatccaa aggatgagct ggtttactat gaagccgatt
    16861 caacatttag cactcatgtt gttaactcca aatcgttgga atacatcttt atattcagca
    16921 gcagcggttc atgctccgag taccgctacc taaagaccag cacacccgaa ggtaaattta
    16981 aggtatttca accccgtatg cccggtgtgg aatacaccgt agagcactcg ggtaactttt
    17041 tctacattat gaccagctgg aatgccgaaa acaataggat tatggaaacg cctctgacat
    17101 ctacccgaaa ggaaagctgg agggaacttg tcccgaacag gagtgatgtg ctggtcatga
    17161 atgtggacat ctttaaggat tttcttgttg ctacagagcg aaaagacgct ttaatccagg
    17221 ttaaggtgat atcactcagg gataaatcgc agcactacat cgattttggc gaggaggtat
    17281 atactgcttt accatcggta aactccgagt ttaacacttc atcatttgtt tacttttaca
    17341 cctcgctcac cacaccggga gtaacctgcg agtacaacat gcaaacccat cagaaaaaaa
    17401 tactcaagca aaatgaggtt cttggtggct ataaccctga taactacgaa acaaagcgtt
    17461 tatgggcaac cgcagccgat ggggttaaca ttcctatttc ggtggtttac cgcaagggca
    17521 ttaagctaga cggaaaaaat ccgttactca tatacggtta tggggcttac ggctacccgg
    17581 taaatcccac cttcgatcct aacctgctaa gcctgctcga taggggcttt gtgtatgcaa
    17641 ttgcacacgt caggggagga caagagctgg gtcgcaaatg gtatgaagat ggcaggaagt
    17701 tatgcaaaat gaacacattt accgacttca ttgcctgcac cgaatgcctt gttgagcaga
    17761 aatacacctc gcctgatagg atagtagcac agggcgcaag cgccggaggc ctcctgatgg
    17821 gggttattgc caaccttcgt cccgatttat acagggcaat tgtagctgaa gtcccctttg
    17881 ttgacgtgct taccaccatg cttaatccta acctaccgct cacaactgga gaatatgatg
    17941 aatggggcaa cccaaacgat tccatagttt accactatat aaaatcatac tccccttacg
    18001 atggcgttag gcagcaggat taccctagca tgcttgttct agccagctac tatgattctc
    18061 aagttcagta ttttgaaccc gccaagtggg tagccaagtt gagagccagc aaaacagata
    18121 acaacctcct acttctaaaa acagatatgg aatcgggtca tgcgggtagt tcgggcaggt
    18181 acaaaactct aaaagatgaa gcttttgtgt acgcatttat acttcacgca gtaggaattg
    18241 aggactagaa tcactctaca gtcgcttcca gctagtcaag ttcaaagtgt ttttttacaa
    18301 gctccagtaa tttttcccgg tttatgggtt tggaaatgta atcattacat cccacgctaa
    18361 gggctttctc cctgtcgttt gtcatggcat aggccgtttc ggcaataata accacgttgg
    18421 agcttacctc tctaattttt ttggttgctt catagccatc catttctggc attttaatat
    18481 ccataagtat tagatcggta tcggggttac taagaaacat gctcacagca tcggcaccat
    18541 tcctggccga tattatttca cctgcaatat ccctcagctg attcttaagt agtattcccg
    18601 aggggacatc atcctcggct ataagaattt tcaacttttt actaaatggt tgacttaccg
    18661 aatcgttttt atcctgattt aaagcattag aataagattg ctgcttacga gcagcaggcc
    18721 gaaccggtat ggtaaaatgg aaagttgaac ctttccctac ttcgctttcc acccaaatac
    18781 tccccccaag gagctctaca tacgattttg caatggtgag ccccaatcca gcaccctcgt
    18841 acctgccaga gtagttggtg ttagcctgaa cgaaccgctc aaatatcttt ccgatagtca
    18901 tagggggaat tccgatgcct gtgtcactca cgtaaaattc caggtactcc tcctttaatt
    18961 tgcaaccaaa cgctacactt ccgttatcgc aatactttat ggcgtttttg actaggttcg
    19021 atagaattac ctgcaacttt aacggatcgg tagtaacagt ggcttgctca tcaggtagct
    19081 ggtattccag aaaaaatttt agccccttac tatcggcgtc gggcttaaaa aagttcaacg
    19141 catactgcag ctgctcgttt acgtcgcatt ggcttaggtt cacctgcact tcacccgact
    19201 caattttaga aatgttgata aggttgttga ttatatcgag cattcgctga ccgctctgct
    19261 caataacgtc aaggtattcc cttacctcct cttccgagag tctcgggttt tttagtagct
    19321 gggcaaaacc aagtattccg ttcatcgggg ttcgaatttc gtggctcatg ttggccaaaa
    19381 atgccgattt tagcctatcg ctctcctccg ccttctcacg tgcggctatt agctcagcct
    19441 cccaattctt ttgctcagtg atgtccctgg aaacagctat caccactttt ctaccgaagt
    19501 actcgccggg agttaggctt acttctttag ggaatatggt gccatcattt cggagacccc
    19561 aaaattcgaa gatttggggt tgaccgtcga aggccttact aacgtaatcg gtaactttat
    19621 ccagatcatt tatctccgga gccgataaat cctttggctt tttccctata aaatactccc
    19681 gcgggtatcc atagtatttt tcgcaggcct tatttacatc caggaaggtg ccatcggcat
    19741 cctgaacata aacagcttcg gatatgctgt tgataatgcc gcggtaggtt ttttcggata
    19801 gggacaggtg ttcctccatt ttctttttct cggttatatc ccttccgctt cctactacgc
    19861 ctataagttt tccattctta tcgtacaggg gtgacttatg cacatcaaga aagatgaact
    19921 ttcccttaac atttccatac tcatcaaact gcatctcctt cagctcacga agggtatcca
    19981 ggtcagagtt catacataat tcaccgaatg tgtgccactc aggattttca ggatgggatt
    20041 ccctttccct tttggcaaaa aacatgtcat ttttgccaac tggttcatcg gtatccctgg
    20101 cgtttagtat ggtgctgcag aatgccttgt tggcaaaaat gtagttttga tttaagtcct
    20161 ttgcccacac catgtcggga atagtgtcgg ccattaacct catcaggttg taaaggttgt
    20221 agtacttttc ctcgctctcc ttaagggcat tctccgcatt tgtacgactg gttacatccc
    20281 ggacaattgc caaaaccttg gaagtgccgt atctaaccat ccggctttca aagaattgtg
    20341 gcttgttatc aatggtaagc tggtagctgt aggtttgcat tttaccggtt ttcagcacct
    20401 cctcaaccac aacccgggca tttgaagcaa caggctcggg cagcacttcc tctatgcgtt
    20461 tgccaagaaa catttcgggt ggaacataga taggtgcatc cttgccgcca tggtagccga
    20521 cataccgtac atcggagtca aaaataaaaa tcatatcggg gattaagcca agcagcgcct
    20581 ggtatttatc ctcaataccg ctactgtcag tattgtctgg tttttccatt ttgagaaaag
    20641 taatgttatt aatttttaca atttaggtaa acgataggag aaagtcaaca ttttttaagg
    20701 gaacatgatg taatagggga taaagtgaaa aagataaagg ataaaaatgt tcaaggttaa
    20761 gaactttttt attaaaagag ttattaagcc ccgaaggggc gaga
//
LOCUS       DAOSHM010000042        19993 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_2186_length_19993_cov_20.638830, whole genome shotgun
            sequence.
ACCESSION   DAOSHM010000042 DAOSHM010000000
VERSION     DAOSHM010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 19993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19993
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2186_length_19993_cov_20.638830"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="PK839_07450"
     CDS             complement(<1..192)
                     /locus_tag="PK839_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72606.1"
                     /translation="MMLTGLSLQDLPGRFPGVIIKNSKGYKEVGFRTRPPNGGFLISE
                     LARLPAELVGNLTELVRLPA"
     gene            269..2050
                     /locus_tag="PK839_07455"
     CDS             269..2050
                     /locus_tag="PK839_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HOG72607.1"
                     /translation="MRPSGLKRQFSRTWHGFRRLGERRIMLILSVVVGVSSGLAAVLL
                     KNVLHVANVFVQESFPKDSANFLFFALPATGILLTIVFVRFLVKDDIGHGVSKVLYSI
                     SKQNGRIKPHNMYSSMVASTSTIAFGGSVGAEAPIVYTGAAIGSNVGNFFRLNYKNII
                     LLIGCGAAGAIASIFKAPLAGIVFTLEVLMLDLTTASIVPLLISAVSAATVSYFFLGR
                     EVVLAYDVVRPFALQKIPYYVLLGVFCGFISLYFIRVVMWIEKRFERITSIYRRWILG
                     AMILGTFTFLFPPLYGEGYEVLQALLDGRSSFIFNSSPFYEFQSNVWLLLVALLILIL
                     AKGVATAVTTGAGGVGGTFAPTLFIGGVAGFFVAKLLNQLGITVVSESNFALIGMAGA
                     MAGVMHAPLTAIFLIAEITGGYALFVPLIITSTISFLTILYFEPYSIYTKNLAKRGEL
                     ITHHKDKAVLTMLSVRKVLETDFTPVEANQTLGDLVKIVGQSKRNIFPVVQDDGQLVG
                     VVTLDDIRSKMFDTSAYTVVHVQDIMTLAPAVIDVDEPMEKVMQKFEDTGAWNLPVTD
                     GGRYIGFVSKSKIFSIYRKMLVKVSDE"
     gene            2324..2722
                     /locus_tag="PK839_07460"
     CDS             2324..2722
                     /locus_tag="PK839_07460"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOG72608.1"
                     /translation="MMIPHDSKTPLLQGKKILIIEDDTVSRIFLSELLQSTHAEVDFV
                     RTAYEAKKYLDTHQAPNAILLDLRLPDMDGFELAAAIHRQHGDVCIIAQTAYVVEGID
                     EKCRQSGIDAHIYKPIRRDNLLDLLGQYVK"
     gene            2792..4072
                     /gene="glyA"
                     /locus_tag="PK839_07465"
     CDS             2792..4072
                     /gene="glyA"
                     /locus_tag="PK839_07465"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007835317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HOG72609.1"
                     /translation="MVRDTKIFDLIEKERQRQMHGIELIASENFVSPQVLEAMGSVMT
                     NKYAEGYPGARYYGGCEVVDQSEQLAIDRLKELFEAEYVNVQPHSGAQANMAVFVAVM
                     KPGDTFLGLDLSHGGHLSHGSPVNFSGMWYKAVSYGVKEETGRVDYDMMERVALEQKP
                     RLIVGGASAYSREWDYKRMRAIADAVGALLMIDMAHPAGLIAAGLLDNPLRYAHIVTS
                     TTHKTLRGPRGGIILMGKDFPNPWGLTTPKGEVKMMSQILNSSVFPGIQGGPLEHVIA
                     AKAVAFGEALRPEFKEYQRQVKLNAATMANAFMDKGYKVVSGGTDNHSMLIDLRTKFP
                     EITGKKVENALVRADITINKNMVPFDSRSPFQTSGIRVGTPAITTRGLKEQHMNHIVD
                     LMDRVISSADDEKTITAVKEEVNKFMRDFPLYAW"
     gene            4231..4713
                     /locus_tag="PK839_07470"
     CDS             4231..4713
                     /locus_tag="PK839_07470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72610.1"
                     /translation="MELFHIKLNEFPYRKSKFFRLLIVAAIILALLAILTILIFVNRQ
                     EKGFVGIILIWVSYFLVYLYWAWYSFRVKLYIHADEFAIKFKFGMVPSLSQVILWETI
                     EKVRLGPTYIAFYKKTGRRKVAKLGWLPYSKVVEIKDKVANACRIKHIPVEVADFTHY
                     "
     gene            4737..5486
                     /locus_tag="PK839_07475"
     CDS             4737..5486
                     /locus_tag="PK839_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOG72611.1"
                     /translation="MEWWIYLLVVLAGVAAGFINTLAGSGSLLTLPLLIFIGLPANVA
                     NGTNRIGILMQSMVAVGRFKQKKVFEWSDGLWLTIPAGLGAVLGALVAVDLNEVLMNR
                     IIGILLVFMFFLLLFEPEKWLKPKNPDSRIRFTPFRFIVFFVIGIYGGFIQAGVGFFL
                     LAGLVLGAGMDLVRSNALKVLITMVFTILALVVFIINGQVDFKLGIIMGIGNMIGAWI
                     ATSVALKHGASFIRWVLLICVLAFGLKLLIW"
     gene            complement(5495..6331)
                     /locus_tag="PK839_07480"
     CDS             complement(5495..6331)
                     /locus_tag="PK839_07480"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HOG72612.1"
                     /translation="MTITANKAKGIAATVLFHILMVLLLMMFGFNIPFPLPAEEGIII
                     NFGNSDEGMGLEEPAIGEQAISNPAGYPTMQEDESPLTQDYEEAPALPVKKEESKKKV
                     AEPTEKTNPASSTDKVAPSTTAATPEVEKPREANAKALFPGQKINGGQTGGEGVTGKP
                     GNQGSLEGSASSTNRTGGATGGGGDGQNGIITNLNGRSALLLPKPDYPKQKEGRVVVE
                     VTVDNHGNVTKAIAGIKGSTTLDTDLLKAAEKAALSAKFDVSSNAPSFQVGTITYIFR
                     LN"
     gene            complement(6335..6739)
                     /locus_tag="PK839_07485"
     CDS             complement(6335..6739)
                     /locus_tag="PK839_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010261039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOG72613.1"
                     /translation="MALRHRSKSDPGLNMSSMTDIVFLLLLFFMISSTLVHPNALRLL
                     LPQSNSQISDKPVTSVSITADRNFYLETVPVSIGQLEYLLQQKMKGENDPTIALHVDR
                     SVPMEDVVKVMNIAKDNGYKLVLATQPIKKEQ"
     gene            complement(6751..7392)
                     /locus_tag="PK839_07490"
     CDS             complement(6751..7392)
                     /locus_tag="PK839_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007833131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOG72614.1"
                     /translation="MGQSGEAKMRFFDMAVKGGWIMIPIFILSIIAVYIFVERYMAIH
                     KASKIDANFMNKIREYIHEDKVEAALALCQSQNTPVARMIEKGIQRIGRPLSDVNSAI
                     ETAGNLEISKLEESMPLLATIAGGAPMLGFLGTVIGMIRAFFDMSMAGNSIEIGLLSN
                     GIYIAMVTTVAGLIVGIPAYFGYHYLVARIEKVVYTMEANISEFMDLLNEPVA"
     gene            complement(7547..12073)
                     /locus_tag="PK839_07495"
     CDS             complement(7547..12073)
                     /locus_tag="PK839_07495"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HOG72615.1"
                     /translation="MLRVVIVLLAVPSLIWLTLQNSRIQTYLVDRVTASLKERLGIEV
                     SVGRVDFRPFASLLLRDVFIADLKGDTLLHSGRIAVTLLGLKLAPSNIELGRANFDQT
                     TFNFVTDSSGVMNLSGLIRKIKETFPSKKDSSDFRLSANSISISNSRFILKKTAPKAR
                     NSDFGMNFQDMNFSNFNLKVDNLYIHRDTVALSINSLNFSDHSGFKLDNLRANFSLCR
                     SNMKFDKLRLQAHDSNIRLNRLNFAFSSWKDFGNFVHSVELDAQFAGTYLKTSTLSYF
                     APVFRKVDLAVSVDGHVKGTISDLRGKGLELRYGSQTLLRTNFNITGLPDIKQTLMVI
                     DLYELSTNREDLAAITNPSSGKPLVNLPQNFDMLSTLTYKGNFTGYLNNFVAYGNLTS
                     AIGSIAVDLSFVPDKSNNIDINGNLRTTQLDVGKFSNSPILGKATLSASLNGTIDNQR
                     KINFFTNATINSLQANSYTYNDIVISGNLTNKTYSGSVRLNDPNCKLNFMGIIDFSDT
                     IPVFDFSAFVPKLDLVKLNLNKKDSVSQASFLLTTKFRGSNLDNSSGEIKVVNSMYRN
                     QHGEFKLDDVTINADNKEDSKVITLQSDFAEGELRSKYNYSKFFTYLNNLVLKYIPAM
                     KPESETESSTVAFTGVEKPEYNDYLVKFRLKKTEKITSVLMPQLSIAENTSIFGILNP
                     DLQTFSFKVRVPQMRFGNTGIKDLAAEGQTKDSLFTASINTPLITIGSSKIENFKISS
                     YLSHNLLNLAIGWQNTQQPASRGLIEATADFTSYNKNGQLTSIAFNPSTFVINDSLWN
                     LSPSTVVIDTASVKFNSFSIRSRNQHFNIDGKLSASNMDTLKVNLLNMDISYLNLYLQ
                     NRGYNVSGTINGFAEINSAYQNPTLFSKLSINKLILNSNPIGNVTFSSTWINSEKRMA
                     VELTNNHEDTLTLKATGNLYTDDGSMDFLVDVNRVKLVHLAPLLKGSVSDLSGNVNGK
                     IRVNGKTSKPMLNGNLSFSNATLTVDFLKSTFTINDKISVDNSNLYFRNFKITDRFNR
                     VATINGDIKTNSFKDINFNIGMAAENFLCMNTTEHDNSQFYGTVFGTGPIFITGKPDN
                     LNFNINLKTEGKTAIYLPISTEKTVKQNNFISFVSSNPDQIVIDEPVATKKSKVNLNL
                     NLDLLVTPDAEVQIIIDKKLGDIIKANGSGNLKMEISPEKDVFRMFGNFDIEKGDYLF
                     TLQGVINKKFTIMPGSSITWNGGPLDATMNIEAAYRVKTSLKQLLMDERYTTRVPVDC
                     KIFLTQKLTSPVIKFGIEVPNADAETKALVDGALNTEEKVNTQFLGLLVINSFIADPN
                     QAGMMTQSSSSMGTMGLYNTASELLSNQFSNWISQWSNSVDIGVNYRPGMESELSSNQ
                     MEVALSTQLLNDRVSINGNVDVGRRSTSSPIAGDFNIDVKLNQSGKLRLKAFARTNDD
                     LLPTNQQNSYTTGVGVLYREDFNNMKDLRRRIRNTFNSEESFSPIIVPENSSQPDSTK
                     DKTSSYPSPFVQFR"
     gene            12235..13254
                     /gene="tsaD"
                     /locus_tag="PK839_07500"
     CDS             12235..13254
                     /gene="tsaD"
                     /locus_tag="PK839_07500"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010261047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HOG72616.1"
                     /translation="MSITILGIESSCDDTSAAVIRDGFLLSNLISSQEVHREYGGVVP
                     ELASRAHQANIIPVVDGALKKAGVGIGEINAVAFTQGPGLMGSLLVGSSFAKGVALSR
                     QIPLVAVNHLQGHVLAHFISDDKAQDHPSFPFLCLLVSGGHTQLIIVRKPLEMEIVGQ
                     TIDDAAGEAFDKCAKVMGLPYPGGPVVDKLAAGGNIHAFRFARPIIKGLDFSFSGLKT
                     SFLYTLRDRVAGDPDFIAKNKADLCASLQHTIIEILLAKVEAAAKKFGVTQVAIAGGV
                     SANSGLRKAMTELGDKHGWQVYLPQLRYTTDNAAMIAITGYYKYLAGEFSPLDIMPMA
                     RIPLL"
     gene            13280..13426
                     /locus_tag="PK839_07505"
     CDS             13280..13426
                     /locus_tag="PK839_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72617.1"
                     /translation="MSWVGLDETCPDTNGTCSTPGGTCPATDGFDDLDNLCGLAKISR
                     PFRA"
     gene            complement(13532..15904)
                     /locus_tag="PK839_07510"
     CDS             complement(13532..15904)
                     /locus_tag="PK839_07510"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOG72618.1"
                     /translation="MKISTISKKHSHTNRINTAGVIVLKKILPLLLLFLVGFLASSAY
                     GKNSNTIVLQLKWKHQFQFAGYYAALEKGFYEKAGLNVEIRELPSNTSAIDEVLSGRA
                     NYGVACSDLLVQYMNGKPLVLLAPIFQSSPSVLLVLASSNIYTPGDLVNKTIELSAKG
                     GGTTDILAMLGVEGIKPSQIKAVSASFSLNRLLNGQVDATEAYLTNEPYFLDKFGIPY
                     RVISPKKYGIDFYAECLFTTQEEVKRNPSRTNRFLEASIKGWEYALQNPEEIVKLIQT
                     KYKSTKTTEHLLNEAQRVKEFIQPDFINIGHSNMGRWKQMTEVLLRAGLISKTRSLNN
                     FVYEPKSTRDTISDLPWIAGIFTAAIIGLLLFHRWRIRKRINKHNLITRQQREQIEQL
                     THEVDMLRKGLETVSLRNNELEAFKDMLLANLSFEIRTPLNTIIGYSELLNGPHVKPQ
                     QTLHYTKEINKSATVLQHQIDNILDLSKLETHQLKLVYQRINLQDLFGTLHALLLNEL
                     RIFDKDHIAIQTFIDEEIDFDILSDRNLIRNIFTKLINNSVKFTPKGYIELGCKKTND
                     AMLHFWVQDSGPGISNSQLETLYEPFTQTNDSNPYGSLRLGLPVVKRLIDFLNGRIWI
                     ETTENLGTTVNFEIPYTPIGTRKRVTAKNGKHFLLQEPPQLKNKTILIVEDIHSNYFL
                     LAKMLEDTGCSIINAKRGSEAIEIFSTNHNVDMVLMDLRLADMDGIEVTRAIRQIDTK
                     VIIVAQTAYSSGVKINLSIEAGCNDFITKPVSRVDLFNILRKYLSPEGAT"
     gene            complement(16164..17351)
                     /locus_tag="PK839_07515"
     CDS             complement(16164..17351)
                     /locus_tag="PK839_07515"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016277912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HOG72619.1"
                     /translation="MNSPRVTLKPGKEQSLKRFHPWIFSGAIKHVEGSPTEGEVVQVY
                     DPDDHFLAVGHFHTGSIAVRVLSFQQEEINTEFWTQRIAEALNLRKSAGLFPSGETSM
                     FRLVHGEGDNLPGLIVDIYGSTAVIQCHSTGMYLARQAIARALIEIFDGNIKAVYDKS
                     SSTLPRRENLHPSDGFIEGEGDTKGTFIENGHQFIVSFEQGQKTGFFLDQRENRHLLQ
                     SYAKGLSVLNTFAYTGGFSVYALAGGASEVTDVESSEAAVKALQQNINLNFSPTTNHK
                     VLIADVFDYLRSTSEFFDIIVLDPPAFAKHKDALKNALQAYKRLNLAAIRRLKPGGLL
                     FTFSCSQVVDKTDFRNAVFSASAISGREISILHQLSQPADHPINIYHPESEYLKGLVL
                     KVK"
     gene            complement(17480..18091)
                     /locus_tag="PK839_07520"
     CDS             complement(17480..18091)
                     /locus_tag="PK839_07520"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOG72620.1"
                     /translation="MFATSISNEEIAKLPRLVFEGETVVVSENNHLERCLAELSNSRL
                     VGFDTESRPSFKKGRSNGISLLQLASSNLAAIIRVKSTGIPPALLTFLEDSSIIKVGA
                     AIRDDLKGLKQIHTFTPRGFIDLQQVVTNYNIENVSVRKMAAIVLGGRVSKSQQLSNW
                     EAPELTTQQVEYAATDAWVCREIYIRLLQEKPGIGERKHTAMF"
     gene            complement(18095..18709)
                     /locus_tag="PK839_07525"
     CDS             complement(18095..18709)
                     /locus_tag="PK839_07525"
                     /inference="COORDINATES: protein motif:HMM:NF028012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5063 domain-containing protein"
                     /protein_id="HOG72621.1"
                     /translation="MDIKATVYGRNTLEFLTVAKEFCSFLEGCESYSTRNFVSASSKL
                     LPLLYYKAALLPETTPIYDEGNEQFVSENDYVAVIQKVEGLLGQHDDFAEFNYQQPEG
                     SFETITASIAEYLADIYQDLKNFTMRYQLGKEEIMNDAIWECRENFGEYWGVRLVALI
                     RAFHTLQFGDVDLDNIDEPEDSSKIDTSSWFITRRQNDSNPFQD"
     gene            complement(18913..19827)
                     /locus_tag="PK839_07530"
     CDS             complement(18913..19827)
                     /locus_tag="PK839_07530"
                     /inference="COORDINATES: protein motif:HMM:NF013761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HOG72622.1"
                     /translation="MMLNKMIAAMLPYLPKKLIWVFSKRYIAGETVNDAIRVARDLNH
                     KGVKVTLDILGEFIKTLEEAEKNKQEYLSLIDIIQKEKIDGNYSVKPTMFGLLIDKEV
                     CYQHLREIVAKAASYNSFIRIDMEDSSCTDLEIELYRRLKSEYPKNVGLVVQAYLKRT
                     LDDLKKLLDLNSKEIPMNYRLCKGIYVEPAEIAFKKYEEVNQHYLEDLEFMFKNGIYA
                     CIATHDKPLVEGAYKLIEKYKVPSNMLEFQMLYGVTPELRDSIVGKGYTMRVYVPFGK
                     QWFGYSTRRLKENPKMTSHIIKALFVRG"
BASE COUNT         4926 a         4247 c         4811 g         6009 t
ORIGIN      
        1 cgccggtagg cggacaagtt ccgtcaggtt accgacaagt tccgccggta gtcgagcaag
       61 ttccgaaatt aggaatccgc cgttaggcgg acgagttctg aaacccacct ccttgtatcc
      121 tttactattt ttgattataa ccccaggaaa tcggccaggc aggtcctgca ggcttaatcc
      181 agttaacatc atgcccggga accaattgct gaaaataatg gctacatttg ctattggtag
      241 caaaatagtt tattcaaaaa tcttttagat gagaccttca ggattgaaaa ggcaatttag
      301 caggacgtgg catggattca gaaggcttgg ggaaagaaga ataatgctga ttcttagcgt
      361 agtggttggc gtttcaagcg ggctggccgc agttcttctt aaaaatgtgc ttcatgttgc
      421 taatgttttt gttcaggaat cgttccctaa ggattcagcc aacttcttat tttttgctct
      481 accggcaaca ggtatcctgc taaccattgt tttcgtgcgt tttttggtga aggacgacat
      541 tgggcatggc gtttcaaagg tgctttactc aatctccaag caaaacggac gaataaagcc
      601 tcataacatg tacagctcaa tggtggccag cacctctact attgcctttg gcgggtcggt
      661 tggcgccgaa gccccaatag tttacacagg ggcagccata ggatcaaacg ttgggaattt
      721 tttcaggtta aactacaaga atattatctt gcttattggc tgcggcgccg ctggagcaat
      781 tgcatcaatt tttaaggcgc cccttgcagg aatagtattc acgctagagg tgctcatgct
      841 cgatttaaca actgcctcta tagttcccct gctcatttcg gcagtgtcag ctgctacggt
      901 ttcctacttt tttttgggtc gggaggtagt tttagcctat gatgttgtac gtcccttcgc
      961 gcttcagaag ataccctact acgtgctgct gggggtgttt tgcggtttca tatcgctcta
     1021 cttcatcagg gtggtaatgt ggattgagaa acgcttcgaa aggattacca gcatataccg
     1081 gcgctggatt ttaggggcta tgattttggg tacttttacc tttttgttcc ctccgcttta
     1141 cggcgagggt tacgaggtgc tgcaggctct actcgatgga cgttcatcgt tcatattcaa
     1201 tagcagcccg ttctatgaat ttcaaagtaa cgtttggttg ctgctggtgg cgcttctcat
     1261 tttaatttta gccaaaggtg tggcaacagc cgttactact ggtgctggtg gcgttggggg
     1321 aacttttgcc ccaacactct ttataggagg ggtggcaggt ttttttgtgg ctaaactctt
     1381 aaaccagctt ggtataactg ttgtgtcgga aagtaacttt gcactgattg gaatggctgg
     1441 cgccatggcc ggggtgatgc acgcacccct aacggccatc ttcctgattg ctgagattac
     1501 tggcgggtac gctctttttg tcccgctaat catcacctct accatttcgt ttctaaccat
     1561 tctttacttc gagccctact ccatctacac aaaaaacctt gccaaaaggg gtgagcttat
     1621 cactcatcat aaggataagg ctgtgctcac tatgctgagc gttcgcaagg tgctcgaaac
     1681 agactttaca cctgttgaag ccaatcaaac ccttggcgac ctggttaaaa ttgttggcca
     1741 atcgaagcgc aacatctttc ctgttgtgca ggatgatggc cagttggtgg gggttgttac
     1801 ccttgacgat atccgctcta aaatgttcga tacttcggcc tataccgttg ttcatgtaca
     1861 ggatataatg actcttgccc cggccgtcat tgatgttgat gaaccgatgg agaaggtgat
     1921 gcaaaaattc gaggataccg gcgcatggaa tctcccggtt actgatggtg gaagatacat
     1981 tgggtttgtt tcgaaatcaa aaatattctc catttaccgc aaaatgctgg tgaaagtttc
     2041 cgatgagtaa acatgtattg tgtaatgttg tttaaaaggt ttgaaccaat caattttttg
     2101 tagtaattta tcttttttat gtttttgaca cgctttgttt tggcgatttc tttttgatgg
     2161 gggtttcagg gtttactctt cggcgattat tgtgctaaat gccaacacgt tgagatttga
     2221 aacaaacatg tagggcagct tgtatgaacg cgtatgaaca accgggtaat atttgcgggt
     2281 tcggctgtga aagggaatta atgagaaacc taaaaatttt gtaatgatga taccacacga
     2341 ttcaaaaacc ccactccttc aagggaaaaa gattttgata atcgaggatg acacggttag
     2401 ccgcattttt ttgagcgagc tcctacagtc aacccatgcc gaggttgact ttgtacgtac
     2461 ggcatacgaa gccaaaaagt atttagacac ccatcaagcg ccaaatgcca tactgctgga
     2521 tttgcgccta cccgacatgg atgggtttga gctggcagcg gctatccacc ggcagcatgg
     2581 cgatgtgtgc ataattgcac aaaccgccta tgtggttgag gggatagacg agaagtgccg
     2641 gcagtcggga attgatgccc atatctacaa gcctatcagg cgggataacc tgctggattt
     2701 actcggacaa tatgttaagt aatatgcagg taagctgatt agcgacagcg ataaaagact
     2761 acatttgcca taaaataata aatcattcag catggtacgc gatacaaaaa ttttcgacct
     2821 cattgaaaag gagcgccaac ggcagatgca tggcatcgag ttaattgcct ccgagaattt
     2881 tgtaagccct caggtgctag aagccatggg ctcagtgatg accaacaagt atgccgaggg
     2941 ctatccgggc gcaaggtact acggtggatg cgaggtggtt gaccaatcag aacagctggc
     3001 catcgaccgg ctgaaggagc tttttgaggc ggagtacgtt aatgttcagc cccactcggg
     3061 tgctcaggcc aacatggccg tatttgttgc ggttatgaaa ccgggcgaca cctttcttgg
     3121 cctcgacctt tcgcatggcg gccacctgtc gcatggttca ccggtaaatt tctcggggat
     3181 gtggtacaaa gcagtttcct atggtgtaaa ggaggaaacc ggacgcgttg actacgatat
     3241 gatggagcgg gtggctcttg agcaaaaacc aaggttgatt gttggaggcg cttcggccta
     3301 ctcccgtgaa tgggactaca agcggatgag ggcaatagcc gatgcggtag gagcattact
     3361 catgatagac atggctcacc ctgcaggcct gattgccgca ggattgctcg ataatcccct
     3421 tcgttacgcc cacattgtta cctcaaccac ccacaaaacc cttcggggcc cacgtggcgg
     3481 aattatcctg atgggtaagg attttcctaa tccatggggg cttactacgc caaaaggcga
     3541 ggtgaaaatg atgtcccaaa tactcaactc cagcgttttc cccggcattc agggtggacc
     3601 gctggaacat gttattgccg ctaaggctgt agctttcggt gaggccctta gacctgagtt
     3661 caaggaatac cagcgtcagg ttaaacttaa tgctgcgaca atggccaatg cctttatgga
     3721 caagggatac aaggtggttt cgggtggcac cgataaccac tccatgctta ttgacctgcg
     3781 tactaagttc cccgaaatta ctggcaaaaa ggtggagaat gcccttgttc gagccgacat
     3841 caccattaac aagaatatgg tgcctttcga cagccgttcg ccattccaaa cttccggtat
     3901 cagggtcggt acacctgcaa ttactacccg tgggctgaag gagcagcaca tgaaccatat
     3961 tgtcgatttg atggataggg ttatctcaag tgctgatgat gaaaaaacaa ttactgccgt
     4021 taaggaggag gttaacaagt ttatgaggga ctttccactt tatgcatggt aggcttgttt
     4081 tacgtatttt gcactcttgt tagtatgaac ctggatttaa tgcacgtggt gtataaaagt
     4141 ggaaccgagc ctgcaggttt ggttccatta ttataacggc gattagctta ccaagctaat
     4201 acatgtctac cactcctagc taacaaaaag atggaattgt tccacattaa gctaaacgag
     4261 tttccctaca ggaaatcgaa atttttcagg ttgctaattg tggcggctat tattttagcc
     4321 cttctcgcta ttttaaccat tttgattttt gttaatcggc aagaaaaggg atttgtaggc
     4381 ataatcttaa tctgggtttc ctatttcttg gtttacctct actgggcatg gtattcgttc
     4441 agggtaaagc tctacatcca tgccgatgaa tttgccatta agttcaagtt tggcatggtt
     4501 cccagcttgt cgcaggtaat tttatgggaa accattgaga aggttaggtt gggccctacc
     4561 tatattgcct tttacaagaa gaccggtagg cgaaaagtgg ctaagttagg ctggctgccc
     4621 tactctaagg tggttgaaat taaggacaag gttgccaacg catgcagaat aaagcacatt
     4681 cccgttgagg tggccgattt tactcattac tgatactttt gatttgttta tttgatatgg
     4741 agtggtggat atacctatta gttgttctgg caggagttgc agcaggtttt atcaacacac
     4801 ttgccggtag tggttcgcta cttaccttac ctttattgat tttcattggt ttacctgcaa
     4861 atgtagccaa cggaaccaac cgtattggaa ttcttatgca gagcatggtt gcggttggta
     4921 ggtttaagca aaaaaaggtt tttgagtggt ccgatggtct ttggctgact attccggcag
     4981 gtctgggcgc tgtgctgggc gcactggttg ccgttgacct taatgaggtt ctaatgaacc
     5041 gaattatagg cattctgcta gttttcatgt tcttccttct gctttttgaa cccgaaaaat
     5101 ggcttaaacc taaaaatccc gatagtcgta ttcgctttac tccgtttcgg ttcattgtct
     5161 tttttgtaat aggaatctac ggaggattta ttcaggctgg cgttgggttc ttccttttgg
     5221 cagggctggt gctgggtgca ggtatggatt tagttagatc gaatgctctt aaggttttga
     5281 taactatggt attcaccatt ttggcgttgg tggtgtttat cattaacgga caggttgact
     5341 ttaagctggg catcatcatg ggcattggca acatgattgg tgcctggatt gctaccagcg
     5401 ttgcgctaaa acatggtgca tcatttattc ggtgggtgtt gctgatttgt gtgctggcct
     5461 ttggcctcaa gctgttaatc tggtagcctt tgtgctagtt tagcctaaag atataggtaa
     5521 tagttccaac ctggaaggat ggggcgtttg acgatacgtc gaatttggca ctcaacgccg
     5581 ctttctcggc agcctttagt agatcggtgt caagagtggt tgaacccttt ataccagcaa
     5641 tagcttttgt aacatttcca tggttatcaa cagttacttc caccactaca cgaccctcct
     5701 tctgcttggg gtaatcgggt ttgggaagaa gcagcgcact tctcccatta aggttggtga
     5761 taattccatt ctgtccgtca ccgcctccac cggttgctcc gccggtgcgg ttggttgagc
     5821 tggcagaacc ctcaagcgat ccctggttgc ccggcttgcc tgtaactccc tctcctccgg
     5881 tttgcccgcc attaattttt tgtcctggga aaagcgcctt ggcatttgcc tcccgtggct
     5941 tttcgacttc gggagttgct gccgttgtgg atggagccac tttgtccgtg ctactggcgg
     6001 ggttggtttt ttccgttggt tcagctactt tctttttgct ctcctccttt ttaaccggta
     6061 aggctggggc ttcctcgtag tcctgtgtta ggggactttc atcctcctgc atggtggggt
     6121 atcccgccgg gtttgaaatt gcctgctcgc caattgctgg ttcctccaat cccatacctt
     6181 cgtcggagtt gccaaagtta ataataatcc cctcttcggc tggtaggggg aagggtatgt
     6241 taaagccaaa catcattaaa aggagaacca taaggatatg gaacagaacg gttgctgcta
     6301 ttccctttgc cttattggcc gtgattgtca tttgttactg ctcctttttt attggttggg
     6361 tagctaaaac cagcttgtat ccattatcct tggcaatatt catcacttta accacatctt
     6421 ccatcggaac cgacctgtca acatgtagtg ctatggtagg gtcattttct cccttcattt
     6481 tctgctgaag aaggtactcc agctggccaa tggataccgg aacagtttcg aggtaaaagt
     6541 tgcggtcggc tgtaatggaa accgaggtaa cgggtttgtc ggaaatctgg ctgttgcttt
     6601 gtggaagtag cagcctcagg gcgttggggt gtactagggt agaggagatc atgaagaaca
     6661 gcagcagcag gaacacgatg tcggtcatgg acgacatgtt aaggcctgga tcggattttg
     6721 aacgatgtct taatgccatg atgtaacgtt ttatgctaca ggttcgttaa gcagatccat
     6781 aaactccgaa atatttgcct ccatggtgta aaccaccttc tcgatccgtg caaccaggta
     6841 atggtagccg aagtaagcag gaatccctac aattaatccg gcaacggtgg taaccatggc
     6901 aatgtatata ccgttagata aaaggcctat ttcgatactg ttgcccgcca tggacatgtc
     6961 gaagaacgca cgaatcatac caataactgt gccaagaaac cctagcattg gggcaccacc
     7021 ggcaatggtg gcaagcaatg gcatgctctc ttcaagtttg gaaatctcca ggttaccggc
     7081 agtttcaatt gccgagttta catccgataa gggcctgcct atgcgttgaa ttcctttttc
     7141 aatcattcgg gcaactggtg tgttttgcga ttggcaaagg gctagtgccg cttcaacttt
     7201 atcctcatga atgtactccc ttatcttgtt cataaagttg gcgtcaattt tagaagcttt
     7261 atgaatggcc atgtagcgtt caacaaaaat ataaacggca ataatggaga ggataaaaat
     7321 gggaatcata atccaaccgc ccttaacggc catatcaaaa aatctcattt tagcctcgcc
     7381 gctttgtccc atacccgttt gggcctgagc tgccgataga agaatagtgg tgagtgtagt
     7441 cattgcttga aatttaattt tgcgaaaatt actaaaaaaa cgtttaaaat gaccagggta
     7501 ttgtttaggc taaattaata acccacaaat aatggggttg ggcgtgctac ctgaactgga
     7561 caaatggaga agggtaggag cttgttttgt cttttgtact gtcgggctgg ctgctatttt
     7621 ctggaacaat aatggggctg aagctctctt cggagttgaa ggtgttgcgt attcgccgcc
     7681 ttaaatcctt catattgttg aaatcttcac ggtagagaac tccaaccccg gtagtgtagc
     7741 tattctgttg attggtggga agcaagtcgt cgtttgtacg ggcaaaggct ttcagtcgca
     7801 gcttacccga ctggtttagc ttaacgtcaa tgttgaaatc acctgcaatt gggctgcttg
     7861 tgcttcgcct acccacatct acattaccgt tgatggatac cctatcgttg agcagctggg
     7921 tagatagtgc cacctccatc tggttggagc tcagctcact ttccatgccg ggtctgtagt
     7981 taacacctat gtcaaccgaa ttgctccatt gcgatatcca gttgctgaac tggttggata
     8041 gtagctcgct ggcagtgttg tacaggccca tggtacccat ggagcttgag ctctgggtca
     8101 tcattccggc ctggttggga tcagctataa aactgttgat taccagcaga cccaggaact
     8161 gggtattaac cttttcctcg gtgtttagcg ctccatcaac cagggctttt gtttcggcat
     8221 cggcgttggg aacttcaatt ccaaacttga ttacgggcga agtgagcttc tgggtgagaa
     8281 aaattttaca gtcaaccggt acgcgggtgg tgtacctttc gtccatcagt agctgcttga
     8341 gcgaggtttt tacccggtac gcagcttcaa tgttcatggt ggcatctaga ggtcctccgt
     8401 tccacgttat gctgctgccg ggcattatgg taaatttctt gttgattacc ccttggaggg
     8461 taaaaaggta gtcccccttt tcgatgtcaa agtttccgaa catcctgaaa acatcctttt
     8521 ctgggctaat ttccattttc agatttccgc taccgttggc cttgatgatg tcgccgagct
     8581 tcttgtcaat aataatctgc acctctgcat cgggggttac cagcaggtcg aggttcaggt
     8641 tgaggtttac ctttgacttt tttgtcgcta caggttcgtc tataactatt tggtcgggat
     8701 tgctgctaac aaaggaaatg aagttgttct gctttacagt tttctccgtt gaaatcggga
     8761 ggtagatggc tgttttgccc tctgttttta ggttgatgtt gaagtttagg ttatcgggtt
     8821 taccggttat aaagattggt ccggtgccaa acactgttcc ataaaactgg ctgttgtcgt
     8881 gctcggtagt gttcatgcag aggaaattct cggcagccat gccgatgtta aagttgatat
     8941 ccttaaagct atttgtcttg atatcgccgt ttatggttgc tacccggtta aaccgatcgg
     9001 tgattttgaa atttctgaag tataggttgc tgttgtcaac ggaaattttg tcgtttatag
     9061 taaaggttga ctttaagaaa tcaacagtta gcgtggcgtt gctaaatgag aggtttccgt
     9121 taagcattgg cttgcttgtt ttcccgttca ccctgatttt cccgtttaca tttccggata
     9181 ggtctgaaac gctacccttt agcaaggggg ctaggtgaac cagcttgaca cggtttacat
     9241 ctaccagaaa atccatactt ccatcgtcgg tgtagaggtt tccggttgcc ttcagggtta
     9301 gagtgtcctc atgattgttg gttagctcaa ctgccatgcg cttttctgaa ttaatccagg
     9361 tgctggagaa ggttacattt ccaatgggat ttgagtttag gattagcttg ttgatgctta
     9421 gtttggaaaa aagcgttgga ttttggtatg cactgttgat ctcggcaaac ccgttaatgg
     9481 ttccgcttac gttgtacccc ctattctgca ggtaaaggtt aaggtatgat atgtccatat
     9541 taaggaggtt taccttgagg gtatccatgt tactggctga aagtttacca tctatgttga
     9601 aatgctgatt tctgctgcga atggagaagc tgttgaattt tactgaggcc gtatcaataa
     9661 ctactgtcga gggggacagg ttccataatg agtcgtttat cacaaatgta gatgggttaa
     9721 aagcaatgct ggtgagctga ccattcttgt tataacttgt aaaatcggca gtggcttcaa
     9781 ttagcccgcg ggaggcgggt tgctgggtgt tttgccagcc tatggcaaga ttcagcaggt
     9841 tatggctaag gtacgagctg atcttgaaat tctcgatttt tgaactacca atagtaatca
     9901 acggtgtatt gatgctggcc gtaaataggc tgtccttggt ttgcccttca gcagctaaat
     9961 cttttatgcc ggtatttcca aaacgcatct ggggtaccct gactttaaaa gaaaaggttt
    10021 gcaagtcggg gttgagaata ccgaatatgc tggtgttttc ggcaatgctt agctgaggca
    10081 tgagaacgct ggtaattttt tcggtttttt taagcctgaa tttaacaagg tagtcgttgt
    10141 attcaggttt ttcaactcct gtaaaggcta cagtggagga ttctgtttca ctctccggct
    10201 tcattgccgg tatatacttc agaacgaggt tgtttaggta ggtaaagaat tttgagtagt
    10261 tatacttact ccgtagttct ccctcagcaa agtccgactg aagggtaata accttgctgt
    10321 cctctttatt gtcggcattt atggttacat cgtctagttt aaattcccca tgttggtttc
    10381 ggtacatcga gttcacaacc ttaatttccc cgctgctgtt gtcgaggttg ctgcccctga
    10441 acttggtggt aagcaagaat gaggcttggg aaaccgaatc ctttttgttg aggttgagct
    10501 ttaccaggtc cagctttggt acaaaggctg aaaagtcgaa tacgggaatc gtgtcggaaa
    10561 agtcaataat ccccataaag ttgagcttgc agttgggatc gttgagacga accgatccgg
    10621 aataggtttt gtttgtaagg ttacccgata ttacaatgtc gttataggtg tagctgtttg
    10681 cctgtaggga gttgatggtt gcgttggtga aaaagttaat ttttctctgg ttgtcaatgg
    10741 tcccgttgag cgatgcgctt agtgttgctt ttccaagaat tgggctgttg ctaaactttc
    10801 ccacatccag ctgtgtagtt ctgagattcc cgttgatgtc tatgttgttg cttttatcag
    10861 gcacgaatga taggtcaacg gcaatactgc caatggcgct ggttagattt ccgtaggcaa
    10921 caaagttgtt gagatagccg gtaaagttac ccttataggt tagggtgctg agcatgtcaa
    10981 aattctgggg aaggtttacc aggggtttgc cggaagaagg gttggtaatt gcagctaaat
    11041 cctcacggtt ggttgataat tcatatagat caataaccat aagggtttgt ttaatgtccg
    11101 ggagcccggt tatgttaaag ttggttcgga gtaatgtttg cgatccgtac ctcagctcaa
    11161 gtcccttacc gcgtaagtct gaaattgtac cctttacatg gccatcaaca ctaactgcta
    11221 aatcaacctt gcgaaacacg ggtgcaaagt aggagagggt gctggtttta aggtatgttc
    11281 ccgcaaattg tgcatccagc tccacgctat ggacaaaatt gccaaaatcc ttccagctgg
    11341 aaaaagcaaa attcagcctg ttgagcctta tgttggagtc gtgtgcctgc aacctcaact
    11401 tgtcaaattt catgttcgac ctgcataagc taaaattagc ccggaggtta tcgagcttaa
    11461 atccggaatg gtcgctaaaa ttaaggctgt ttattgaaag tgctacggta tcacgatgaa
    11521 tgtataggtt gtcaaccttc aggttgaagt tgctgaagtt catatcctga aagttcatgc
    11581 cgaaatccga atttctggct tttggagctg tttttttcag tataaacctg gagttgctaa
    11641 tagaaatact gttagcgctg agcctgaaat ccgatgaatc tttttttgag ggaaaggttt
    11701 cttttatctt tctaattaac ccgctaaggt tcataacgcc cgacgaatcg gtaacaaagt
    11761 tgaaagtggt ctgatcgaag tttgcccttc ccagctcgat gttgctgggt gccagtttaa
    11821 ggccaagcag ggtaacggct atcctgccag aatgtaggag tgtgtcgccc tttaggtcgg
    11881 caataaaaac atcccttaga agcaagctgg caaatgggcg aaaatcaacc ctgcctaccg
    11941 atacctcaat tcctaagcgc tctttaaggg aggcggttac ccggtcaacc agataggttt
    12001 ggatccggct gttctgaagg gttaaccaga ttaatgaggg tacggccagc aggacaatga
    12061 ctacccttaa caatattttt gctgtttttt tgataccttt gcctatattt atgggtatag
    12121 taaacctaaa tttaattctc aaacttcgtt ccgaaagtaa gcaattagaa atgttttttg
    12181 gaattgtcca ttccgaatgt ttcaatcaat ttgatggttt aataaaaatt aacgatgagc
    12241 ataactattc tggggattga atcgtcatgc gatgacacct ctgcagcggt gattcgcgat
    12301 ggattcttgc tctccaacct aataagcagc caggaggtgc atagggagta tggaggcgtt
    12361 gttccggaac ttgcctcgag agctcaccag gcgaatataa ttccagtagt tgacggagcg
    12421 ctaaaaaaag ccggtgtggg tattggcgaa atcaatgcag ttgcctttac acagggtccc
    12481 ggcttaatgg gttcgctcct ggttggaagt tcgtttgcca agggagtagc cttgtcgcgt
    12541 caaattcccc ttgttgcagt taaccatttg caggggcatg tgcttgccca cttcatcagc
    12601 gacgacaaag ctcaggatca cccatcgttc ccattcctat gcctgctggt ttcgggtggg
    12661 catactcagc ttattattgt tcgtaagccc cttgagatgg agattgtagg gcaaactatt
    12721 gatgatgctg caggcgaagc tttcgacaaa tgtgccaagg tgatggggtt accctacccg
    12781 ggagggcctg tggttgataa gctggctgcc ggggggaaca ttcatgcttt tagatttgcc
    12841 cgtcccataa ttaaaggact tgacttcagc ttcagtggac ttaaaacgtc gtttttatat
    12901 accctgcgcg atagggttgc tggcgaccct gattttattg caaaaaacaa ggccgattta
    12961 tgcgcatcgc ttcaacatac cattattgag attcttctgg ccaaggtgga ggctgccgcc
    13021 aaaaaatttg gagtaacgca ggtggccata gctggaggtg tatcggcaaa ctccggcctg
    13081 cgaaaggcca tgaccgaact cggtgataaa cacggctggc aggtttacct accccagctt
    13141 cgctatacaa ccgataacgc cgccatgatt gccattaccg gttactataa gtatctggca
    13201 ggagaatttt cacctctgga tattatgcca atggctcgaa tacctttgtt gtaaagggat
    13261 aaggagtaag ctaaaaggtt tgagttgggt cggactggac gaaacctgcc cggataccaa
    13321 cggaacttgc tccactcctg gtggaacttg tccggctact gacggattcg atgacctgga
    13381 caacctttgc ggacttgcaa aaatatcccg ccctttcagg gcttaatgct gctggagtag
    13441 ttttgttcaa ggggcttcac ccaacctaat tgaatccgtc caccttttcc gggtacacgc
    13501 cacccagttc aatgttaaca gttcaacatg gttaagttgc cccttcgggc gaaaggtatt
    13561 ttcgtaaaat gttgaaaagg tcaactctac ttaccggttt ggtaatgaaa tcgttgcaac
    13621 cggcttcaat gctaaggttt attttaaccc cggagctgta ggctgtttgc gcaactatta
    13681 tgacttttgt atcaatttgc cggattgcac gggttacctc aattccatcc atatcggcaa
    13741 ggcgtaggtc cattagcacc atatcaacat tgtggttggt gctgaaaatt tcaattgcct
    13801 cgcttcccct tttggcattt atgatactgc atcctgtatc ctcaagcatt ttggctagca
    13861 gaaagtagtt ggaatggata tcctcgacta taagtattgt tttattctta agctgaggcg
    13921 gctcctgaag gaggaagtgc ttgccgtttt tggctgttac cctttttcgg gtaccaattg
    13981 gggtgtaggg gatttcaaaa tttacggttg ttcccagatt ctcggtcgtt tctatccaaa
    14041 ttctaccgtt cagaaaatca ataagtcgtt ttactaccgg aaggccaagt ctgaggcttc
    14101 cgtaaggatt cgagtcgttt gtctgagtaa atggttcata gagtgtttca agttggctgt
    14161 tacttattcc tggtccgcta tcctgcaccc agaaatgaag catggcgtcg tttgttttct
    14221 tgcaacccag ctcaatatac cccttgggtg tgaacttaac cgagttgttt atcagctttg
    14281 tgaaaatatt cctaatgagg tttctatccg aaaggatgtc aaaatcaatc tcttcgtcaa
    14341 tgaaggtttg aatggcaata tgatccttat caaatattct aagttcattt agcagcaatg
    14401 cgtggagggt accaaacaaa tcctgcaggt tgatgcgctg gtacaccagc ttgagctggt
    14461 gggtttctag ttttgaaagg tctaaaatgt tgtcaatttg atgctgcagg acggtggcgc
    14521 tcttgttgat ttcctttgta tagtgcaagg tttgttgtgg tttaacgtgt ggcccgttca
    14581 acagctcgct atagccaatg atggtgttca ggggtgttct gatttcgaac gataggttag
    14641 ccaaaagcat atccttaaag gcttcaagtt cattatttct tagcgacacg gtttccaggc
    14701 cttttcttag catatctacc tcatgagtaa gctgctcaat ttgctctctt tgctggcggg
    14761 ttataaggtt gtgtttgttg attctttttc tgattctcca gcgatgaaaa agcaaaagtc
    14821 cgataatggc agctgtaaaa ataccagcaa tccagggtag gtcagatatt gtatccctgg
    14881 tacttttagg ctcatacaca aagttgttca ggctacgtgt tttgctaatc agaccagccc
    14941 taagcagcac ttcggtcatt tgcttccacc ttcccatgtt ggagtggcca atgttgatga
    15001 agtctggctg aatgaactcc ttaacccttt gagcttcatt cagcaggtgc tcggttgttt
    15061 tggttgattt atacttggtc tgtatgagct taactatctc ctcaggattt tgaagggcgt
    15121 actcccatcc tttaatgctg gcttccagga agcgattcgt cctcgatgga tttcttttaa
    15181 cttcttcctg ggtggtgaaa aggcattcgg cataaaaatc tatcccatat tttttaggtg
    15241 aaataaccct gtagggtatg ccaaacttgt cgaggaagta gggttcgttt gtcaggtaag
    15301 cctccgtggc atcaacctgg ccgtttaaaa gcctgttgag cgagaaggaa gcgctgacgg
    15361 cttttatttg tgatggctta atgccctcaa cacccagcat ggcaagtata tcggttgtac
    15421 cgccaccctt tgcgcttagc tctatggtct tgttaaccaa gtcgcccggg gtgtaaatat
    15481 tgctgctggc aagaaccagc aaaaccgacg ggctgctttg gaaaattgga gcgaggagaa
    15541 ccaaaggctt gccattcatg tactggacaa gcaagtcgga gcaggcaaca ccgtaatttg
    15601 ccctgcccga caacacctcg tcaatagccg aggtgttgga tggaagttcc cttatctcga
    15661 cattcaatcc ggccttctcg taaaatccct tttcaagtgc tgcgtagtac ccggcaaact
    15721 ggaattgatg cttccatttt agctggagaa caatggtgtt ggagtttttt ccgtaggctg
    15781 atgacgctaa aaaacctacc aggaatagta atagaagtgg tagtattttt tttaatacaa
    15841 taacacccgc ggtattaatg cgattggtat gggaatgctt tttagaaatg gttgaaatct
    15901 tcacgtcggg ctagctaaaa gaatcattat gatttacact tttacgcaat gttaaagtta
    15961 cactatttat taaaaaaaat ctgcatcagc ttttaaaaaa ttacaactta cacggcctaa
    16021 cgggtaggcg ttaaattgct tcaaattcaa gtgtggccgg caattcggat gttagaatgt
    16081 ggtgctaaaa tataaaagtt gggaattttc tgaaaaacct attttaatgt ataatgattt
    16141 gcaaatgttg tatcaggttg gagctacttc actttaagaa ccagcccctt gaggtattca
    16201 ctctcagggt ggtaaatgtt tataggatga tctgctggtt gtgagagttg atggagaatg
    16261 gatatctcac ggcccgaaat ggcactggcc gagaatacgg cattcctgaa atcggtttta
    16321 tccaccacct gcgagcagga aaaagtaaat agcaaacctc caggtttcaa ccttcggatg
    16381 gcagccagat tcaagcgctt gtaggcttgc agggcatttt tgagtgcatc cttatgcttg
    16441 gcaaaggccg gagggtcaag aactataatg tcgaagaatt cgcttgttga ccgcaggtaa
    16501 tcaaaaacat cggctattag tactttgtgg tttgtagtgg ggctaaagtt gaggttgata
    16561 ttttgctgca atgcctttac agctgcttcg gaactttcaa catcggttac ctcggaagcc
    16621 ccacctgcca aggcgtaaac cgagaagcct cctgtatagg caaaagtgtt aagaacgctc
    16681 aaacccttag cgtaactttg tagcagatga cgattttcgc gctggtcaag aaaaaatccg
    16741 gttttctgcc cctgctcaaa cgaaacgatg aattggtggc cattctcaat aaacgttcct
    16801 ttagtgtcgc cttccccttc aataaatcca tcggagggat gtaagttttc gcgtcgggga
    16861 agggtgctgc tgcttttatc gtaaactgcc tttatgtttc cgtcgaaaat ctctataaga
    16921 gcgcgggcaa ttgcctggcg ggcaagatac atacctgttg aatggcactg aattactgcg
    16981 gttgaaccgt atatgtcaac tatgagcccg ggtaagttgt cgccctcgcc atgcaccagc
    17041 ctaaacatgc tggtttctcc gcttggaaaa agcccggcag atttccttag gtttagggcc
    17101 tcagctattc gctgagtcca aaattcagta ttgatttctt cctgctgaaa gctgagaacc
    17161 ctaacagcaa ttgaacctgt atggaaatgc ccaaccgcca gaaagtggtc gtccgggtca
    17221 taaacctgaa ccacctcgcc ttcggttggc gaaccttcta catgtttaat ggcaccggaa
    17281 aatatccagg gatggaatcg ttttaacgat tgctccttgc ctggtttaag cgttactcgg
    17341 ggtgaattca tggtcattta ataggtaaaa taaaaacata aatggagaag ctatgtaaaa
    17401 gattagggct aaagctaagg ttatgcctgg ttgtatgctg tggagttgcg ttattcccaa
    17461 taaacactca aaaatgcgat taaaacattg ctgtatgctt acgttcgcct atgcctggtt
    17521 tttcctgaag tagcctgatg tagatctccc tgcaaaccca cgcatcagta gcggcatact
    17581 ccacctgctg ggtggtcagc tcgggtgcct cccagttgct cagctgctgg ctctttgaaa
    17641 ctcttccgcc caaaactata gccgccattt ttctaacgct cacgttctcg atgttataat
    17701 tcgttacaac ctgctgaaga tcaataaatc cacgaggtgt gaatgtgtga atttgcttca
    17761 acccctttaa atcgtctctt atggctgcac ctaccttgat gattgatgaa tcttcgagaa
    17821 aagtcagaag tgcaggcgga attcccgtgc ttttaactct aataattgct gcaaggtttg
    17881 aagaggctag ctggagcaga gagattccat tgcttctacc ttttttaaat gaaggtcttg
    17941 actccgtgtc gaatcccact aagcgactat tcgaaagttc agccaggcac ctctccaggt
    18001 ggttattttc cgagaccaca acagtttcgc cctcaaatac cagtcggggt agtttcgcta
    18061 tctcttcgtt gctgatgctg gttgcaaaca ttatctaatc ctgaaacggg ttactatcgt
    18121 tctgacggcg ggtaataaac cagctcgacg tgtcaatttt tgaggaatct tccggttcat
    18181 caatattatc caaatccaca tccccgaatt gaagggtatg gaaagcccgt atgagcgcca
    18241 caagtctcac tccccagtac tcgccaaaat tttcgcggca ttcccagatg gcatcattca
    18301 taatctcctc cttaccgagc tggtagcgca tggtaaaatt ttttaaatcc tggtagatat
    18361 cggccagata ctcggcaatg ctggcggtaa tcgtttcaaa agaaccctcg ggttgctggt
    18421 agttaaattc agcaaaatcg tcatgctggc ctagtaatcc ttcaactttt tgtataacag
    18481 caacatagtc gttttcggaa acgaattgct cgttaccttc atcgtaaata ggcgtagtct
    18541 cgggcagtag ggcagccttg tagtaaagca gcggcagaag cttgctgctg gccgatacga
    18601 aatttctggt tgagtagctt tcgcaacctt ccagaaagga gcaaaactcc ttggccaccg
    18661 ttaaaaactc caacgtatta cggccgtaaa cagttgcttt tatatccatc tctcaccatt
    18721 atgagctaca aagatagcta ttgtggtgag gtcttttgta ggatagctta caaataaacg
    18781 aatgacagca tttttaacag cctaacgatg gctgaaactg cccaatttgt tttggtacag
    18841 tagcacataa acccggtagg gtggtgaaaa ttgtggagag ttttagtcgg gaatttctga
    18901 atgcaggtgg tgctaccctc taacaaacag ggctttaata atatggctgg tcatttttgg
    18961 gttttccttc agcctacggg tggagtagcc aaaccattgc ttcccgaatg gaacgtaaac
    19021 cctcatggta tatcccttac ccacaattga gtctcgaagt tcaggagtta caccgtaaag
    19081 catctggaat tcgagcatat tgctggggac tttgtacttt tctatcagct tatatgcacc
    19141 ttcaactagg ggtttgtcat gggttgcgat acaggcgtat attccgtttt tgaacatgaa
    19201 ctccaaatct tccaggtaat gctggtttac ttcctcgtat tttttgaatg ctatttctgc
    19261 gggttctacg tatatgccct tgcaaaggcg gtagttcatg ggaatttcct ttgaattcag
    19321 gtcaagcaac tttttcaaat catccagggt tctctttagg taggcttgta ccaccaaacc
    19381 aacatttttg ggatattctg acttgagcct ccggtatagc tctatttcca ggtcggtaca
    19441 tgatgagtct tccatatcta ttctgatgaa actgttgtag gaagcagcct tcgcaacaat
    19501 ttcgcgtaaa tgttgatagc aaacttcctt atcaatcagt agtccaaaca tggttggctt
    19561 tacggagtag ttaccgtcaa ttttttcttt ttggatgatg tctatcagcg aaaggtactc
    19621 ctgcttgttc ttttccgcct cttcaagagt tttgatgaat tcccccagta tgtcgagggt
    19681 aacttttacg cccttgtggt tgagatctcg ggcaaccctt atggcatcat taacggtttc
    19741 accggcgatg tagcgcttgg aaaaaaccca aatcagcttt ttaggaaggt agggcagcat
    19801 ggctgcaatc attttattca gcatcatttt ttaattttta tgttgaaaac gagaatatgg
    19861 ttaagctatg aagtccaact tgtacatata aagttcaaaa ggtttaaaaa gttcaaagtt
    19921 ttgatgttta aaagttgatg atgaacatct tgtttttgga tttctttacc cttcccttgt
    19981 cttcagcccc aac
//
LOCUS       DAOSHM010000043        19580 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_2248_length_19580_cov_19.475647, whole genome shotgun
            sequence.
ACCESSION   DAOSHM010000043 DAOSHM010000000
VERSION     DAOSHM010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 19580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19580
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2248_length_19580_cov_19.475647"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(822..2141)
                     /locus_tag="PK839_07535"
     CDS             complement(822..2141)
                     /locus_tag="PK839_07535"
                     /inference="COORDINATES: protein motif:HMM:NF028426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5103 domain-containing protein"
                     /protein_id="HOG72623.1"
                     /translation="MHLNSKILMLAMLIPCLTNRCSCNAYSQPVKWSLWDGIALENFS
                     AQSIQSVQVYRTGGELNNPVVELNNADETLTLEFDDITGSYIHYDYTLIHCGWDWKPS
                     GLLFTEFADGFEFNTIDPYGYSTGTILSYTHYNLTLPNEKVSFRISGNYILRVVDSNN
                     HDSIVIQQRFMVVEPIISMNAMVRLPANPKLQTTSQQLELEVLHSPLGNIDPTTDIVT
                     LIAQNNPTHPSMRCKPTFIDRGFTRYTDPDALVFSGGNEFRHIDLKSLHYQGQSISNI
                     AIRDNEYHVQLAPDESRSIQKYSDNPDINGKFIVKRDDSYDSDIEAEYVWAYFTLQVN
                     EDVSEDVYVFGELSGWTVNKGYRLNYNAVTRAYEQRILLKQGYYNYQYAMLDGATGSF
                     YPYVFEGDFSQTENIYLVTVYYRQPGVRYWRLVGIKTLNSRYSDKTR"
     assembly_gap    2503..2602
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2603..4654)
                     /locus_tag="PK839_07540"
     CDS             complement(<2603..4654)
                     /locus_tag="PK839_07540"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOG72624.1"
                     /translation="MRISTYIALIATGIIALLPLRSAYPQGIMDSQQVIADIIEDLTS
                     KAETDEDYSQLAEDLLELSENKINLNSAKKDDLLKLIFLSDFQVQSLLDYRDSTGSIL
                     SIYELQAVPGFDLVDVQRLLPFVKVENAEQAVTASELLGGRNEISIRFKTPLETSAGY
                     SDSYSAASRYTGSKNSYYTRYTYSGGKHFQVGLIAEKDAGEPMFDGTFKTGFDYLSGN
                     IVLSDVGRVKKLVVGDYHAEFGQGLTFWNGISFGKSGNVLSGHKRGRGLVRHSSAYES
                     QYLRGVGLTMPVLKADLTIFGSYRHIDAGIADTLDDGDLAFSSLPETGYHRTTSEIAN
                     RNTLQEMVAGANLTYNLRAVKTGLTFFHSEIEGVNAKTLAIYQLEPIRATKNALGMNV
                     DAYLGKHLLFGEVAAELPTMKLAGIAGGFFRLTNTVQLAMIGRMYARDFNPRYTNGFA
                     EGSGVSNERGLYTGLSVLPAKGWKLTTYVDIFKFPWLRYQVNAPSEGREFMAQSDAVI
                     AENFSLTIRYRGKEAPKNLSGSTAQVTPVVGQTTQSLRLQLGYLPLETVRLKTTLEAS
                     AFSNDSLATEKGYLVAQDISLNPKRFPLSVNARFAVFDTPSWNSRIYSYESDMLYSFT
                     VPAYYSKGTRLFVMVKYSLKNRLDVWLRYSQTYFSELDELGQGADLVEANTRSEIKAM
                     VR"
     gene            complement(4657..5487)
                     /locus_tag="PK839_07545"
     CDS             complement(4657..5487)
                     /locus_tag="PK839_07545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72625.1"
                     /translation="MSGRVVTTMAILMLFSTAKASIPDKPTGGQGAAMGQAWVALNDS
                     WAGFRNQAALAVNRNFWAGIHHENRFLTSELGLSTLGVLIPVSPGTFGVDISHFGYSQ
                     LNTTRIGLSYGMKFSEKFMAGIGLAYHRMQVAGEYKDRNAFTVEGGILYAPVEKLTLG
                     AYIFNPTRSSFDDSQSLPPIIGAGATFRPSQKVVLTLQVDDDTESSPNVRGGLEFTAT
                     KNFCFRLGYSSGNPEGLTGGFGWMVKDINFDLSFGYHRILGYTPQLSVSYCPGSKKNS
                     "
     gene            complement(5515..6027)
                     /gene="purE"
                     /locus_tag="PK839_07550"
     CDS             complement(5515..6027)
                     /gene="purE"
                     /locus_tag="PK839_07550"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005928702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HOG72626.1"
                     /translation="MTPKVSIIMGSVSDMPIMEDAAKFLDELNIAFEINALSAHRTPE
                     MVMEFAQNAHTRGVKVIIAGAGGAAHLPGVIAASTPLPVIGVPIKSSNSIDGWDSILS
                     MLQMPAGIPVATVALNGARNAGILAAQIIATGEPEMLKVMVDFKKSLGEKVVKANQEL
                     ANVKFKCKTN"
     gene            6206..6745
                     /gene="hpt"
                     /locus_tag="PK839_07555"
     CDS             6206..6745
                     /gene="hpt"
                     /locus_tag="PK839_07555"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005657966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HOG72627.1"
                     /translation="MKQVTLKDRTFQLSYPEADIQKDIDVTASKINWDYRNASDIPLF
                     LCILNGSFMFAADLLKRITFPCEVSFIKLASYQGDTSTGVVNHLIGLNESISGRTVII
                     VEDIVDTGVTIAQLIDELQKMNPKEVKVATMLHKPDAYKGSIKIDYIGRSIPNDFIVG
                     YGLDYQGLGRNLPDIYTVI"
     gene            7054..7629
                     /locus_tag="PK839_07560"
     CDS             7054..7629
                     /locus_tag="PK839_07560"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006848788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HOG72628.1"
                     /translation="MLNIILFGPPGAGKGTQSQKIIEKYNLQHLSTGDMLRSAIQQNS
                     PLGLKAKEFMDKGQLVPDEMVIDLIASRIDANGSTKGFVFDGFPRTTLQAAKLDRMLE
                     QRKTPITLLISLEVDYDELIARLVNRGKVCDRTDDQDENVIRGRIDVYNKTTLPVLDY
                     YKAQGKLRSVSGMGSIDEIFERICHIIDCVV"
     gene            7669..8661
                     /gene="obgE"
                     /locus_tag="PK839_07565"
     CDS             7669..8661
                     /gene="obgE"
                     /locus_tag="PK839_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HOG72629.1"
                     /translation="MSNPNFIDYVKIYCRSGQGGAGSVHFRREKYIPKGGPDGGDGGR
                     GGSIYIRGNQQLWTLLHLKYKRHIFAGDGESGSGARCTGKDGKDEIIEVPVGTIAKNV
                     ETGEVIAEVVSDGQTKIIMKGGRGGQGNWHFKSPTMQTPRFAQPGEPCEEGYVILELK
                     ILADVGLVGFPNAGKSTLLSVVSAAKPKIADYPFTTLTPNVGIVGYHDDRSFVMADIP
                     GIIEGAHEGKGLGLRFLRHIERNSMLLFMVPADSEDVLHQYSVLVNELRQYNPELLDK
                     KRALAITKCDLADEHVRKSISLNLPDVPSVFISSVTGEGIERLKDLIWSNINTD"
     gene            8665..9222
                     /locus_tag="PK839_07570"
     CDS             8665..9222
                     /locus_tag="PK839_07570"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOG72630.1"
                     /translation="MVDFLLQLDTKLFLALNGWHSPFWDSIMLFASGKLSWLPLYLLL
                     VFFMVRRFGWKTLWWLLAAAVVVLLADQVTGHAFKYVYHRLRPCHNPDLSSVIHLVGR
                     CGGKYGFISAHAANSFGVATLVSFIFYKRWATIGMLVWATFVSYSRIYLGVHYPGDVL
                     VGAIVGSAIGIAVWLVAERLINRRN"
     gene            9336..11186
                     /locus_tag="PK839_07575"
     CDS             9336..11186
                     /locus_tag="PK839_07575"
                     /inference="COORDINATES: protein motif:HMM:NF025064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HOG72631.1"
                     /translation="MDTELKINEVEVKPHELKVSVNVKEINKRIENIHRYLNSHKEYL
                     NRLNVFPVPDGDTGLNLTLTFQGAMASMQGMENESMLPGEYLKNFTEQMLLNSRGCSG
                     VIFSLFAQGVAQVLENNDFSNENLHRAIANGFKNAWDGTENPREGTMLTLMREFRDIF
                     GKYIQEEKNAAMVISKSIPYLMEVLDRTPEMLPVLKQAGVVDSGGAGFIIILQGIDRE
                     LKHNGSGFNSLSIATLLNISRTTRKLLRMRKSHVRKSSLTPLITNIDDSKIHNSRLRE
                     ILQNARKVINTWNGSRKLPGQEKVISDLEEMGNLWDSDIKLKYCTEFVLEANAINSKE
                     ELREIIGHYGDSLIIVNSGNRYKVHIHTNKPDAVFADAGRYGELVFTKVDDMKKQHRN
                     FVSDDVIEYERDKSILCIVSGSGFADILKNFGADDVLCYGKNKPSVNQLVKKIDGLKA
                     KNVIAAADDSDILMALKYAVSLCKSNVYIVESNNPISIIAMMLGISKEFDIATMFESA
                     MSNLNSITYCEIARSVRNVEADGFTVKKRDFFAVHNKKIIISRTNLEQLVVDVVEKLN
                     PGASLVTIYKGIPSRSDRSLLPILREKFPNLEFEEYYGGQSKFNYYISFE"
     gene            11199..12077
                     /locus_tag="PK839_07580"
     CDS             11199..12077
                     /locus_tag="PK839_07580"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HOG72632.1"
                     /translation="MSQKIFVISADNLGLSRDQVDYPDLELLRFPVFVGDKEYRQDEV
                     HNAKWLLQKYTNEQVVAKSSTLVKQEMVDIVERVRSSYDMIVQVVMGSGMSSASFVVA
                     ENVRKMYEGIIPIVNVDSSQVINGVGNVLLGVIDLIKSNPNLSPEEIAQKSKDVVQNT
                     FTYFIFPDLNYLYRGGRIGKAKSLVGTIMKIIPIIGMLGDNGEGVMVPVGKGRTFKQV
                     NTQLVELIKAKMAEKSASTIKRMNLTHFGGNDEAVAELKEMVGKLPCNDFIIGTPNLV
                     EAVYAGPGAYCLSMSL"
     gene            12090..12707
                     /locus_tag="PK839_07585"
     CDS             12090..12707
                     /locus_tag="PK839_07585"
                     /inference="COORDINATES: protein motif:HMM:NF014690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HOG72633.1"
                     /translation="MNNYIVNLICLVVGYLCGSVLFANIITRLVVGKEIRELGNNNPG
                     AYNVFNNAGKFWGVFTGILDGLKAFVPMLVAHRYFNVSNTGIGLIGVGAVIGHGYPLF
                     FKFKGGRAASSLMGMFLFFIPYELLAAFAIDLILLYTIVKKDFGFWGPFGIIAIAATL
                     CLFFNHPVEVKILIWVGGLLGVYFNRKFIPLRTKQLLNKAPEDDN"
     gene            complement(12777..14780)
                     /locus_tag="PK839_07590"
     CDS             complement(12777..14780)
                     /locus_tag="PK839_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HOG72634.1"
                     /translation="MAATGFSTGGCKSGNEQAEPFKWQVDQFADLRIMRYQIPGFDSL
                     TPKQRVLVYYLSEAAKCGRDIIWDQNYKFNLDVRSVLEGVYQGYKGNRNDPRWGSFMV
                     YLKRVWFSNGIHHHYSTDKIMPDFDRSYFAELVGNTPSEMLPKKFGGVDSILAHFTPI
                     IFDADVAPKRVNQLAGSDLVTTSACNYYEGVTQAEAEEFYSRLANPNDSTPISYGLNS
                     KLVKKNGKIEEQVWYANGMYGKAIGKIVFWLEKAATVAENEYQQKIIETLVDYYKTGD
                     LRKFDEYNILWLSDTQSKVDFVNGFIETYGDPLGYKGSWESVVNFRNDEATHRTEVIS
                     ANAQWFEDHSPIDSIFKKKEVKGVSAKVITVAQLGGDCYPSTPIGINLPNADWIRKQH
                     GSKSVTMDNITYAYNQASLGNGFMEEFAWSDEEVALNKQYGYLADCLHTDLHECLGHG
                     SGQLAPGVKGDELKNYGSPLEEARADLFALYYIMDPKMVELGLMPNLDVARVQYSNYI
                     RNGLMTQLTRVEPGKNIEQAHMRNRQLIAQWCYEYGKDENVVERKNRNGKTYFVVNDF
                     QKLRQLFGSLLREIQRIKSSGDYESGKKLVESFGVKVDPALHHEVLERYSKLNLAPYG
                     GFINPDLVPVVQNDSIVDVRVDYPEDYARQMLEYSERYSFLTK"
     gene            complement(14847..15773)
                     /locus_tag="PK839_07595"
     CDS             complement(14847..15773)
                     /locus_tag="PK839_07595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72635.1"
                     /translation="MRKVILINVAAALVILPLVGFGQEVNANFFSTREDSLSKLLSLI
                     VSETSTTRRDSLNRIFHENFKNTLEHDGAIDYPFSSLKHVGMLTSSDQKVRIITWNIP
                     QTGGYHRYFGFVLVRLDGDKSRLYALTDSREMVKDPQHERLPADRWLGALYYQLAELP
                     YGSEKQYVLLGFDYNNLFTSKKVIEVLTLSPQGEPVFGLPVFNVDNKQLLSRIVFEFS
                     ARAGFNLKYYDEQKIIAFDHLSPSQPEFTGDFQFYGPDASIDGFAFENGKWVYVRDLD
                     MRNPSREKVNPVDAPEKIDEPSFLYKPQQKRK"
     gene            complement(15777..16970)
                     /locus_tag="PK839_07600"
     CDS             complement(15777..16970)
                     /locus_tag="PK839_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HOG72636.1"
                     /translation="MQNIRQLFLSHVAQTSDAPLMLEVSRAEGVFLFGPSGEKYYDLI
                     SGISVSNVGHANPRVVKAVQDQASAYMHLMVYGEFIQSPQVLLAAKLSSMLPQTLSSV
                     YFVNSGSEAVEGAMKLSKRFTGRTEIISFRNAYHGGTQGALSVMGVESQKRSFRPLLP
                     DISILEFNSIADLARITNRTACVIIEPVQGEAGIRVADGNFITELRRRCSEVGALLIF
                     DEIQTGMGRTGKMFALERFGVTPDILLLAKALGGGMPLGAFISSQKIMNCLMENPSLG
                     HITTFGGHPVSCAASLAAIDVLTGDRLPESVPEKEQIFRDILSKHPLVKEFRSFGLLM
                     AIELGSFEKVSALIKYGVEHGFVLDWFLFCNTAIRLAPPLCITKEQCEEVANLIYQGL
                     NEINR"
     gene            complement(16989..17699)
                     /locus_tag="PK839_07605"
     CDS             complement(16989..17699)
                     /locus_tag="PK839_07605"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOG72637.1"
                     /translation="MNCIQVDDDELSRRVIEDYASKIDFIRLTGSFPNAVDALNFLKN
                     SLEPVNLIFLDIEMPEMSGIDFLEATSQEPQVIIISSKEKYALKAFDYSVTDYLLKPI
                     TFPRFTKAVSKAYDKFNQQRSPYDDDKEIFIKKNNMLIRVKYSDILWIEALENYIVIN
                     TVNERFTIHLTMKSIENQLSSVRFKRIHRSFIVNVGKIESIEDNATVIRTPEGRKIIP
                     IGKSYRDKLLSEINLINK"
     gene            complement(17753..18469)
                     /locus_tag="PK839_07610"
     CDS             complement(17753..18469)
                     /locus_tag="PK839_07610"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOG72638.1"
                     /translation="MSGIDHLIQSDRQFRLQLTSYLSDLVLERRLNLLREVLSRRTRY
                     ITVVLEDLYQPQNASAALRSCECLGIQDVHIIENRNSFNVNPEVDMGASKWLTIKKYS
                     GNKSITQEALQTLRSSGYRIVATSPHSLGCSIRDFDTEKGKFAILFGTELLGLSEEAM
                     SLSEEFITIPMYGFTESYNISVSVAITLYELVHRAKANPAYPLSEYEKEVVLLDWLRK
                     SVKNCHIVEKRFVAEYNKLH"
     gene            complement(18746..19018)
                     /locus_tag="PK839_07615"
     CDS             complement(18746..19018)
                     /locus_tag="PK839_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010535670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOG72639.1"
                     /translation="MNKAQLIDAIAAEAKVTKADAKKALDAFIKTTGTALKKGDRVAL
                     VGFGSFSVAKRSARTGRNPQTGKPINIKAKKVVKFKAGSELNGKVQ"
     gene            19485..19573
                     /locus_tag="PK839_07620"
     tRNA            19485..19573
                     /locus_tag="PK839_07620"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19519..19521,aa:Ser,seq:tga)
BASE COUNT         5011 a         4297 c         4674 g         5498 t
ORIGIN      
        1 aacttgtcct cctactggcg gattcaccaa actttaaaaa tggttttaaa aagcggcatc
       61 gtccggcgag ccgaaaaaca agcgcaggcg gcgttaaaaa aaatctgctg ttcgaagcgc
      121 tgagcggagc gaggcgcaag ttcagatttt ttagccgcca agcgtagctt tttcgtgcga
      181 ggcgggcgca gccttgactt tttttgcata cttttttgtg tcaaccgaac cgccgtcagg
      241 cggtgagctc gccgaacacc ttaaaaaatg agctaggggt gaggcaacaa aaaaagtatg
      301 tgggatttgg ggcaaagccc caagatttca tgataataaa cttatccagc ttttaacaag
      361 ctcacaaatt catttcgccc tttcagggct taatacatct tacttctata cttcaagggg
      421 ctacacccct tcctagctat tccgccccgt tggggcttaa gaaaaggata aagggtaaag
      481 gataagaata ttcaagatta agaacttttt tattagaaga gtttttaagc cccgaagggg
      541 ctgaatcttt gtagtcccgg gcgtaagccc ggggttgatg agggtcggcg gtggttggcg
      601 atgcaagtag cggctttccc aaggtgcaat ttcaatcctt tgcatcgcaa aggagaaaat
      661 tttaccagga gcaaggtaaa attaaacata aaagaaaatc tttccacctc cctttacccc
      721 tcgacaagtt cggaattctt aactctttac ctgttcaaag ggttcatttt gaacagccgc
      781 tggatgctga aatcaaacga tgttgcctat attgcttcta attaccttgt cttatcgctg
      841 taccttgagt ttagagtttt aatcccaaca agtcgccagt atcttactcc gggttgcctg
      901 tagtaaactg ttaccaagta gatattctcg gtttgagaga agtctccctc aaaaacatac
      961 ggatagaaac taccggttgc tccatcaagc attgcatact ggtagttgta gtatccctgt
     1021 ttaagcagga tgcgctgttc gtaagctctt gtaacggcgt tatagttgag cctgtaaccc
     1081 ttatttacag tccaaccgct tagctcacca aatacgtaaa catcctcgct gacgtcctcg
     1141 ttgacctgaa gggtaaagta agcccatacg tactctgcct caatatcgct atcgtagcta
     1201 tcatcacgct ttacaatgaa cttgccgttg atgtccggat tatccgagta cttctgaatg
     1261 cttctgcttt catcaggagc aagttgaaca tggtactcgt tgtccctgat ggctatatta
     1321 ctaattgatt ggccctggta gtgtagcgat tttaggtcga tatgcctaaa ctcattccct
     1381 ccggaaaaaa ccagcgcatc gggatcggtg tacctggtaa atcctctgtc tataaaggtt
     1441 ggcttacagc gcatagaggg atgggtgggg ttattctggg cgatgagggt aacaatgtcg
     1501 gttgtgggat ctatattgcc aaggggtgag tgtagtactt ccagctccag ctgctgcgaa
     1561 gtggtttgca gcttcgggtt ggcaggtaac cgaaccatgg cgttcattga tataattggc
     1621 tcaacaacca taaagcgctg ctggattacg atactgtcgt ggttgttgct atcgacaact
     1681 ctcagaatat agtttccgga tattctaaag ctaacctttt cgtttggaag tgttaggttg
     1741 taatgggtat aggataggat ggtgcctgtt gaataaccat aaggatcaat ggtgttaaac
     1801 tcaaaaccat cggcaaattc agtaaaaagc aaacccgatg gtttccaatc ccaaccgcaa
     1861 tggataaggg tatagtcgta atggatgtag ctgccggtaa tgtcatcaaa ctccagggtt
     1921 agggtttcat cggcattatt tagctctaca acagggttgt taagttctcc gcctgtccgg
     1981 taaacctgca ccgattgtat ggattgtgcc gagaagtttt caagtgctat gccatcccat
     2041 aaagaccatt ttactggctg gctgtaggca ttgcagctgc acctattggt taagcagggt
     2101 atcagcatgg ccaacattaa aattttgcta ttgaggtgca ttgaccggtg ggtttgtggt
     2161 ttactggagc aaagttattt tttatttgct ttgtttgaat gttgacaaag catctaaggc
     2221 tatagaattc gtttgtttgc gtacgaccca ttagacattc tgatggatac cttaaaatag
     2281 ttaacagcag caaaatgaaa gtcatttttg atttaaaagt tgatgcaacc tttaaccatt
     2341 attaacagca ctttaacgtg cgttagctat tgatgggctt atatttgcag tgtatttaaa
     2401 aacaaaagtt tctcataggc ttttggtttt agagttaggt ttaggtttgg ttttaggttt
     2461 aggggttaga aacaggggag gggcgatgag cccctctttt gcnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nncctcacca tcgccttaat ctcgcttcgg gtattagcct
     2641 ctaccaggtc ggcgccctgt cccagctcgt ccagctcgct gaagtaggtc tgtgagtacc
     2701 gcagccacac atccaaccga ttttttaagc tgtacttgac cattacaaaa agtcgggttc
     2761 cttttgagta gtaagctggc actgtaaaag agtagagcat atcgctttcg taggagtaaa
     2821 tgcgactatt ccacgatggg gtatcgaata cggcgaagcg tgcatttaca cttaagggga
     2881 aacgtttggg gtttaagctg atgtcctgtg ccaccaggta gcctttctcg gtggcaaggc
     2941 tgtcgttgct gaatgctgat gcctcaagtg tggtttttag ccttaccgtt tcgaggggaa
     3001 ggtagccgag ctgcaacctt aggctctgcg tggtttgtcc cactacaggt gtaacctgtg
     3061 cagttgagcc cgacaggttc tttggtgcct ccttgcccct gtaccttatg gtgagggaaa
     3121 agttttctgc tattacagcg tccgattgag ccatgaactc cctaccctcc gatggggcat
     3181 tcacctggta tctcagccac ggaaatttaa agatatcaac ataggttgtt agcttccaac
     3241 cctttgcggg gagaacggat agcccggtgt ataaccctct ttcgttcgat acgccgcttc
     3301 cctcggcaaa tccattggtg tatcgggggt tgaagtctcg ggcgtacatc ctgccaatca
     3361 tggctagctg aacggtgttg gtcagccgga aaaaaccgcc cgcaatacct gcaagcttca
     3421 tcgttggtag ctctgctgca acctctccaa acagcaggtg tttacccaaa taggcatcaa
     3481 cgttcatacc tagagcgttc tttgttgcgc ggatgggctc cagctggtag atggcaagcg
     3541 ttttggcgtt aaccccctca atttctgagt gaaagaatgt taagcccgtt ttcactgccc
     3601 tgaggttgta ggttaggttc gcaccggcta ccatctcctg taaggtgttt cggtttgcaa
     3661 tctcggaggt tgttcggtgg tagccggttt cgggaagcga cgaaaaggcc agatcaccat
     3721 catcaagcgt atcggctatg ccggcatcaa tgtggcggta ggagccaaaa atggttaaat
     3781 cggcctttag aacaggcatg gtaagaccaa ctccccgtag atactgactc tcataagctg
     3841 acgaatgtcg caccagccca cgtccacgtt tatgccccga aagaacatta cccgattttc
     3901 caaatgagat gccgttccaa aaggtgagtc cctgcccaaa ctcggcgtgg tagtcgccca
     3961 ccaccagttt tttaacccta cccacatccg aaaggactat attccccgag aggtagtcaa
     4021 acccggtttt gaatgtccca tcaaacatgg gttcgccagc atccttctca gctattaaac
     4081 ctacctgaaa gtgctttcct ccgctatagg tgtaacgggt atagtaggag tttttgctcc
     4141 ccgtgtaccg tgatgctgcg gagtatgaat cggagtaccc ggccgatgtt tccaaaggcg
     4201 ttttaaaccg aatgctgatt tcatttctac cgcctagcag ctcgctggca gttacagcct
     4261 gttcggcatt ttcgaccttt acaaaaggaa gtaatctttg aacgtcaacc aaatcgaatc
     4321 ccggaacagc ttgtagctcg taaatgctaa gtatggagcc ggttgagtcc ctgtagtcca
     4381 gcaggctctg tacctgaaaa tcgctgagga agattagctt tagcaggtca tccttttttg
     4441 ctgagttcag gttgattttg ttttcggaca gctcaagcaa atcttcagcc agttgggagt
     4501 agtcctcgtc ggtttctgct tttgaggtaa gatcctcaat aatgtcggct atcacctgct
     4561 gggagtccat gataccctgc gggtatgctg acctgagggg aaggagggca ataattccgg
     4621 ttgcgattaa ggcaatatat gttgaaatcc tcatggctag gagttctttt tgctacccgg
     4681 gcagtagctc accgatagct gcggggtata gcctaaaatc ctgtgatagc caaaggaaag
     4741 atcaaaattt atgtccttaa ccatccatcc aaaaccaccg gttagccctt cagggtttcc
     4801 agatgagtag ccaagccgga agcagaaatt cttagttgcc gtgaactcga gaccgcccct
     4861 cacgttagga ctactttcgg tgtcatcatc aacctgaagg gtaagtacaa ctttctggct
     4921 gggtctgaat gtggcccctg caccaattat tggcggcagc gattggctat catcgaatga
     4981 ggatcgggta gggttaaaga tgtaggcgcc aagggttagt ttctctaccg gggcatatag
     5041 gattccacct tcaactgtga aggcattcct gtccttgtac tcacccgcca cctgcatccg
     5101 gtggtaggca agtccgattc cggccatgaa cttctccgaa aatttcatgc catacgacaa
     5161 gccgatgcgg gtggtgttta gctggctgta gccgaaatgc gagatgtcaa ccccaaaagt
     5221 gccaggcgaa acaggaatca ggactccaag tgtgctcagc ccaagctcgc tggtcagaaa
     5281 cctgttttcg tggtggatac ctgcccagaa gttgcggttt acagctaacg cagcttggtt
     5341 cctgaaccct gcccagctgt cgttcagggc aacccatgct tgccccatag cagccccttg
     5401 gcctccggtt ggcttatcgg gaatggaggc tttagctgtt gaaaacagca ttaggatggc
     5461 cattgtggta accactctcc cggacatggc aggtccacct tggtgtggaa gtttctagtt
     5521 tgtcttacac ttgaatttca cgtttgccaa ctcctggttg gcttttacca ccttttcgcc
     5581 tagcgatttc ttgaagtcaa ccattacctt taacatttcg ggttcgccgg tagcaatgat
     5641 ttgggcggcc aaaatgccgg cgttgcgtgc cccgttgagg gcaactgttg ccacaggaat
     5701 gccggcaggc atttgaagca tggacagtat cgaatcccaa ccatcaatag agttggacga
     5761 cttaatggga acaccaataa ccggcaatgg ggttgatgcg gcaattacac ctggcaggtg
     5821 tgctgcaccc cctgcaccgg caataatcac ctttacgcct cgggtatgag cgttttgggc
     5881 aaactccatc accatctcgg gtgttcggtg ggccgacagg gcgttaattt caaaggctat
     5941 gttcaactcg tccagaaact ttgcagcgtc ttccataatc ggcatatccg aaacgctgcc
     6001 cataataatg cttacctttg gtgtcattta taaatggctg attagttttc aacaaagata
     6061 aaattttttt cgaaatatgc cttcctgttt gctgccatgt tcaataaatc ccaatttact
     6121 tatggttcag ttttaaattt aaaaaatcgg gctaattttg ttgcataatt actgagtaat
     6181 ggatgataat agctataaaa gtcaaatgaa gcaggttacg ctgaaggata ggacattcca
     6241 gctaagctac cccgaggcag acattcagaa ggatattgat gtaacggctt ccaaaattaa
     6301 ctgggactat cgaaatgctt ccgatatccc gttatttctt tgcatactca atggttcatt
     6361 catgtttgcc gccgacttgc tgaagcgcat tacttttccc tgtgaggtct cgtttattaa
     6421 gttggcctcc taccagggcg acacctcaac tggagtggta aatcatttaa ttggcttaaa
     6481 tgaaagcatt tccggtcgca cagtgattat tgttgaggat attgtggata ccggggtaac
     6541 tattgcccag ctcattgatg aacttcagaa aatgaaccca aaagaggtaa aggtggctac
     6601 tatgctccat aaacctgatg cctacaaggg aagcataaaa atagactata ttggtcgttc
     6661 tatccccaac gattttattg ttggctatgg cttagactac caagggcttg gccgtaacct
     6721 acccgatatt tacacggtta tttgatggtt ttcttggagg gttcgggtga atgttgaggc
     6781 ctgaaagtta atgttgggac gctcgaacag cgagccgtaa gccctgaagg gtgaaagtta
     6841 ttgggaaagg gaaggggaaa tatgctgtta aacttgggcc agctaagtgt taaatggttg
     6901 gggatgaatt atttgaatct attacagccg agattttttg aatccgtcag gagccggaca
     6961 agttccgtca ggaggcagat aggtttatta tcttttatcc tttaaccttt atccttttac
     7021 cttgattttt aaccttaatg ctaacatacc gccatgctta acatcatctt gttcggacct
     7081 cccggggccg ggaaaggaac tcaatcgcag aagataattg aaaagtataa ccttcaacat
     7141 ttatcaactg gcgatatgct gagatcggcc attcagcaga acagcccttt aggtttaaag
     7201 gccaaggagt ttatggataa aggccagctt gtacccgatg agatggttat tgacctgatt
     7261 gcctccagga ttgacgctaa tggtagcacc aagggttttg tattcgatgg ttttcctcgg
     7321 acaacccttc aggcagccaa gctcgaccgt atgctggaac agagaaaaac gccaatcacc
     7381 ctgttaattt cccttgaggt ggactacgat gagctcatag cccggctggt taacaggggt
     7441 aaagtttgcg accgaaccga cgaccaggac gaaaatgtca ttcgtggtag aattgacgtt
     7501 tacaataaga caaccctgcc agttctggat tactacaagg ctcaggggaa actgcgtagc
     7561 gtgagcggca tgggctctat agatgaaatt tttgagcgaa tctgtcatat tattgactgc
     7621 gttgtttagc ataatgcttc cgcaaccaca cattatacct gttgcaagat gtccaatccc
     7681 aactttattg actacgtaaa aatctattgc cgctcgggtc agggcggcgc tggaagcgtt
     7741 cacttccgcc gcgagaagta tattcctaag ggtggccctg atggaggtga tggcggaagg
     7801 ggtggctcca tctacataag gggaaaccag cagctgtgga cgttacttca tctgaaatat
     7861 aaacgacata tttttgcagg tgatggcgaa tcagggtcag gggcaagatg cactggtaag
     7921 gacggaaagg atgaaattat tgaagttccg gttggtacta ttgcaaaaaa tgtcgaaaca
     7981 ggtgaggtta ttgccgaggt ggtgagcgat ggccaaacta aaataataat gaaaggcggt
     8041 cgtggtgggc aagggaactg gcatttcaag tcgcctacca tgcaaacgcc ccgttttgcc
     8101 cagccaggtg aaccctgcga ggagggctac gtgattctgg aacttaaaat tttagccgat
     8161 gtggggctgg taggatttcc caacgccggc aaatcaaccc tgctatcggt ggtaagtgcc
     8221 gcaaaaccta aaattgcgga ctatccattt actaccctta ctccaaatgt aggaattgtt
     8281 gggtatcacg atgataggtc atttgttatg gccgatattc ctggaataat tgagggtgcg
     8341 cacgagggaa aaggtttagg acttaggttt ttgagacaca tcgagcgaaa ctccatgcta
     8401 cttttcatgg ttcctgctga ttccgaggat gtgctgcacc agtacagcgt tctggtcaac
     8461 gagcttcggc agtataaccc cgagctactc gataagaaac gtgccttagc tattaccaag
     8521 tgcgacctgg ctgacgagca tgtccgaaaa tcgattagcc taaacctacc agatgttcct
     8581 tctgttttca tctcatcggt tacgggagag ggaattgagc ggctaaagga cttaatatgg
     8641 agtaacatta atactgatta gcaaatggtt gactttctgc tgcagcttga cacaaaacta
     8701 tttctagcgc ttaatggttg gcactcaccc ttttgggaca gcataatgct ttttgcttcg
     8761 ggcaagctta gctggttgcc gctctacctg cttctcgttt tctttatggt aaggagattt
     8821 ggctggaaaa cgctttggtg gctgttggcc gctgcggttg tcgtgctgtt ggccgatcag
     8881 gtaacagggc acgcctttaa gtatgtttac cataggctgc gtccttgcca taaccccgac
     8941 ctaagtagtg taattcacct ggtaggacgg tgtgggggga agtatggatt catctcagcc
     9001 catgccgcca attcgtttgg tgtggccacg ctggtaagtt tcatattcta taagcgctgg
     9061 gctactattg gaatgttggt ttgggcaact tttgtctcct acagccgaat atacctcggc
     9121 gttcattatc ccggcgatgt tctggtggga gctattgtag gttcagcaat aggtattgcc
     9181 gtgtggctgg ttgcagaaag gctcataaac cgaagaaact agggatattt tcaatttaaa
     9241 tatttttatt tttgccggag ggcttacgtt agaagtttta aaagtttatc cgcacattca
     9301 taaaagcaaa ccaaattgac aattagctgg ttgttatgga tacagaacta aaaataaatg
     9361 aggtagaggt aaaacctcac gaattgaagg taagcgttaa tgttaaggag ataaacaaaa
     9421 ggattgaaaa tattcatcgc tacctgaaca gccataagga gtacctgaat aggctaaatg
     9481 ttttcccggt acccgacggc gataccgggt taaacctaac gctaaccttt cagggtgcaa
     9541 tggccagcat gcagggtatg gagaacgaat ccatgcttcc cggcgagtat cttaaaaatt
     9601 ttaccgagca aatgctgctg aatagccggg gttgttcagg tgtgattttt tcattgtttg
     9661 cccagggagt tgcccaggtt ctggaaaata atgatttttc aaatgaaaac ctccacaggg
     9721 ctattgccaa tggtttcaaa aacgcctggg atggaaccga aaatccccgc gagggaacca
     9781 tgcttaccct gatgcgggaa tttagggata tatttggcaa gtacattcag gaggagaaga
     9841 atgctgctat ggtaatcagc aagtcgatcc cctacttgat ggaggtgctt gacagaaccc
     9901 ccgaaatgct ccccgtgctt aaacaagcgg gcgtggttga ctccggaggt gcaggtttta
     9961 tcatcatcct ccagggtatc gaccgtgagc taaagcacaa cggttcgggt tttaacagct
    10021 tgtccatagc taccctgcta aacataagcc gaaccacccg taagcttttg cggatgagaa
    10081 agtctcatgt tcgcaaaagt tcccttacgc cacttattac caatattgat gatagcaaaa
    10141 ttcacaactc ccggttgcgc gaaattcttc agaatgcacg aaaggttata aacacttgga
    10201 atggtagccg aaagttgcct ggccaggaaa aggtaataag cgatttggaa gaaatgggaa
    10261 atctctggga ctccgacata aaattaaagt actgcaccga atttgtgctg gaggccaatg
    10321 ccatcaactc taaggaagaa cttagagaaa ttattggtca ctatggcgat agcctaatta
    10381 ttgttaacag cggtaaccgg tataaggtgc atatccatac caataagccc gatgctgttt
    10441 ttgccgatgc tggcaggtat ggcgagctgg ttttcaccaa ggtggacgac atgaaaaagc
    10501 agcaccgcaa ctttgtgagc gacgatgtta ttgaatatga aagggataaa agtattctct
    10561 gcatcgtctc cggcagcggg tttgctgata ttcttaagaa ttttggtgcc gatgacgtgc
    10621 tgtgctatgg aaagaacaag ccctcggtta accagctggt aaaaaagatt gatgggctaa
    10681 aggccaaaaa cgttatagca gctgccgatg atagcgacat cctgatggcg cttaagtacg
    10741 ccgtttcgct ctgtaaatcg aacgtgtata ttgtggagtc gaataaccct atttccatta
    10801 ttgccatgat gcttggaatt tcgaaggagt tcgacattgc caccatgttc gaatcggcca
    10861 tgagcaacct gaactctatt acctactgcg aaatagccag gtctgtcaga aatgttgagg
    10921 ccgatggttt tacagttaaa aagcgagatt tctttgccgt tcacaacaag aaaattatta
    10981 tttcccgaac caacctggag caacttgtgg ttgacgttgt tgaaaagcta aacccaggag
    11041 caagtctggt tacgatatac aaagggattc cttccaggag cgatagaagc ctgctgccaa
    11101 tccttaggga aaaatttcca aacctcgaat ttgaggagta ctacggagga caaagtaagt
    11161 ttaactacta tattagcttt gaataaactt taaaaactat gagccagaag atatttgtga
    11221 ttagtgccga taacctcggg ctttcgaggg atcaggttga ctaccccgat ttggagctgc
    11281 tcaggttccc ggtatttgta ggggataaag agtaccgaca ggatgaggtg cacaatgcca
    11341 aatggctttt gcagaagtac accaacgaac aggtggttgc caaatcatcc acgctggtga
    11401 agcaggaaat ggtggatatt gtggagcggg ttcgttcaag ctatgacatg attgttcaag
    11461 tggtgatggg aagcggcatg tcaagcgctt cgtttgtggt tgccgaaaac gtgcgcaaga
    11521 tgtacgaggg cattatcccg atagtgaatg tggacagcag ccaggtgata aatggtgttg
    11581 gtaatgttct acttggcgtt attgatttaa ttaagagcaa tcccaactta agtcctgaag
    11641 aaattgcaca gaagtcgaag gatgttgtgc agaatacatt tacctacttc attttccctg
    11701 accttaacta cttgtatagg ggaggaagaa tcggaaaagc taaatcgctg gttggaacca
    11761 ttatgaaaat tatacccatt ataggtatgc taggtgataa cggcgaaggt gttatggttc
    11821 ccgttggaaa gggtagaaca ttcaagcagg tcaacaccca gctcgttgag ctgataaagg
    11881 ccaaaatggc cgaaaaatct gcatcaacca ttaagcgcat gaaccttacc cattttggtg
    11941 gaaacgatga agcggttgcc gagctaaagg aaatggttgg gaagttgcca tgcaacgact
    12001 ttattattgg taccccgaac ctggtagagg cagtatatgc cggacctgga gcttactgct
    12061 tgtccatgtc actataaatt tttaatgtta tgaacaacta tatagttaac cttatttgcc
    12121 tggttgtggg ttacctgtgc ggttcggtgt tgtttgccaa tattatcacc aggctggttg
    12181 taggaaagga aattagggag ttggggaaca acaaccccgg ggcctacaat gtttttaaca
    12241 atgctggtaa gttctggggc gtttttacgg gcattctgga tgggctaaag gcctttgttc
    12301 ccatgctggt ggcccatagg tactttaacg tttccaatac tggtataggg ttaataggag
    12361 taggcgcggt gattggacat ggctacccgc ttttcttcaa gtttaaaggg ggtagggcag
    12421 cctcatcgct catgggcatg tttctctttt ttatacccta cgagctgctg gcagcctttg
    12481 ccattgacct tatactgctc tacaccattg ttaagaaaga ttttgggttc tggggacctt
    12541 ttggaataat cgccattgcg gcaacccttt gcctgttttt caaccatccc gttgaggtga
    12601 aaattttaat ctgggtgggt ggtttgctag gcgtttactt caacaggaaa ttcatccccc
    12661 tccgaaccaa gcagttgctc aataaggcgc ccgaggatga caattagcta aaattggtac
    12721 ttgctgtggt cagtcgcatg gggtcatcag ccggtaggct gatgacccct aatggtttac
    12781 ttcgttagga acgagtagcg ttcggagtac tccagcatct gcctggcata atcctctgga
    12841 taatcaaccc taacgtccac aatgctgtcg ttttgaacta caggcacaag gtctgggttg
    12901 ataaatcccc cgtagggggc caaattcaac ttggaatagc gctccagaac ttcgtggtgc
    12961 agcgcggggt caacttttac cccgaagctc tcaacaagtt ttttgcccga ctcgtaatcg
    13021 cccgaagact taatccgttg aatttcacgc agaaggctgc caaatagctg ccgtaacttc
    13081 tggaaatcgt ttaccacaaa gtaggttttc ccatttcggt ttttcctctc aacaacattt
    13141 tcatcctttc catactcgta gcaccattgg gcaatcagct ggcggttgcg catgtgtgcc
    13201 tgctcaatgt ttttcccagg ttccacccgg gttagctgcg tcattaatcc gttgcggatg
    13261 tagttgctgt actgaaccct ggctacatca aggttgggca tgaggcccag ctcaaccatt
    13321 tttggatcca tgatatagta aagggcaaat aggtcagccc gagcctcctc taatggggag
    13381 ccgtagtttt tcagctcatc acctttaact cctggagcca gctggccaga gccatgccca
    13441 aggcattcgt gcaaatcggt gtgcaggcaa tcggccaggt aaccatactg cttgttgagt
    13501 gctacctcct cgtccgacca ggcaaactcc tccatgaatc catttcccaa cgaggcttgg
    13561 ttgtaggcgt aggtgatatt atccatggta accgattttg aaccatgctg ctttcttatc
    13621 cagtctgcat ttggcaggtt tatgccaatg ggggttgacg ggtagcaatc gcccccgagc
    13681 tgggccacgg ttatcacctt ggcgcttacc cctttcactt ccttcttttt gaatatgctg
    13741 tcaatgggcg agtgatcttc aaaccactgt gcgttggcgc ttatcacctc ggtacgatgg
    13801 gttgcctcgt cgttcctgaa gttcaccacc gactcccagc ttcccttgta gcccagcgga
    13861 tcgccgtaag tctcgatgaa accgttgaca aaatcaactt tagactgtgt atcgcttagc
    13921 cagagtatgt tgtactcgtc aaactttctc aaatcgcctg ttttgtagta atctaccagg
    13981 gtttcaatta ttttttgctg atattcattt tcagccactg tggcggcctt ctccagccag
    14041 aaaacgattt taccgatagc ctttccgtac atgccgtttg cataccaaac ctgctcctct
    14101 atttttccgt tttttttcac cagtttagag tttaaaccgt aggatatggg agtgctatcg
    14161 ttggggttgg ccagcctgga gtagaattct tctgcttcgg cctgtgtaac accctcgtag
    14221 tagttgcagg ctgaggtggt taccaaatca gatccggcaa gctggttaac tcgttttggg
    14281 gctacgtccg catcgaaaat tatgggtgta aaatgtgcca gaatgctatc gacgccccca
    14341 aatttcttgg ggagcatctc ggacggggtg ttaccaacca gctcggcaaa gtagcttcgg
    14401 tcgaaatcgg gcataatttt atcggtggag tagtggtggt gtatgccgtt ggagaaccaa
    14461 acccgcttca ggtaaaccat aaagctaccc catcgtgggt cgttgcggtt tcccttgtaa
    14521 ccctggtaaa ctccctccag aactgagcgc acatcgaggt tgaacttgta gttttggtcc
    14581 caaatgatat ccctaccgca tttagctgct tcactaaggt agtacaccag caccctttgt
    14641 ttaggtgtga gcgaatcgaa gcccggaatt tggtagcgca tgatgcggag gtcggcaaac
    14701 tggtcaactt gccacttaaa aggttcggcc tgctcgtttc ccgatttaca tccaccagtc
    14761 gagaacccgg tagcggccat taacaatagt gccattattt ttctgttcat ttttagttga
    14821 gttgcaaaat gtatgaggtg tgtatgctat tttctctttt gctggggttt atatagaaat
    14881 gagggttcgt ctatcttttc tggtgcgtca acaggattta ctttttctct ggaggggtta
    14941 cgcatgtcca ggtccctaac ataaacccat tttccatttt caaaggcaaa accatctatt
    15001 gaggcatcag gcccgtagaa ctgaaaatca ccggtaaatt cgggttggga gggcgacagg
    15061 tgatcgaatg caattatttt ttgctcatca tagtacttga ggttgaaacc ggctcttgcg
    15121 ctaaactcaa aaacaattct cgaaagcagc tgcttgttat ccacgttaaa aactggcagg
    15181 ccaaaaacag gttctccctg ggggctgagg gtgagcacct cgattacctt ttttgaggtg
    15241 aagaggttgt tgtagtcgaa tcctagcaac acgtactgct tttctgagcc gtagggtagt
    15301 tctgccagct ggtagtacag agctccaagc cacctgtcgg ccggtaacct ttcgtgttgc
    15361 gggtccttta ccatttcccg gctatccgtg agggcataaa gtcgggattt atctccatcc
    15421 agtcgaacca gaacaaaccc gaagtagcga tggtagccac ctgtttgggg aatattccag
    15481 gtgattatcc ttaccttttg atcactggaa gtgagcattc ccacgtgttt tagtgagctg
    15541 aatgggtaat ctattgcccc atcatgctca agagtgtttt taaagttctc atggaaaatt
    15601 ctattcagcg agtcgcgccg ggtggtggat gtttcgctta cgatgagtga gagcagctta
    15661 gaaagcgagt cctcgcgggt agaaaagaag ttggcattaa cttcttgtcc aaaccccaca
    15721 aggggtagaa taaccagggc agccgcaacg ttaattagga ttactttgcg cataagctag
    15781 cggtttattt cgtttagacc ttggtagatt aggttggcca cctcttcgca ttgctccttt
    15841 gttatgcaaa ggggaggtgc aagcctgatg gccgtgttgc aaaatagaaa ccagtccagc
    15901 acgaaaccat gttccactcc atattttatt aaggcgctga ccttctcaaa cgaacctagc
    15961 tctatggcca tcagcagacc gaatgaccga aactctttaa ccagcgggtg tttcgatagg
    16021 atatccctga atatttgctc cttttccgga acgctttcgg ggagcctgtc gccggttaaa
    16081 acgtcaattg cggcgagcga tgcggcacag gaaaccggat gacctccaaa ggttgtaata
    16141 tgcccaaggg aagggttttc catgaggcag ttcattattt tctgtgagga aatgaatgcc
    16201 cctaggggca taccaccacc cagcgccttt gctaggagca ggatgtcggg tgtaacccca
    16261 aatcgctcta gcgcaaacat ctttccggtt cggcccatgc ctgtttgaat ctcgtcaaaa
    16321 atcagtaaag cgcccacctc ggagcaacga cgacgtagtt cggtaataaa attgccatcg
    16381 gcaaccctta tgcccgcctc gccctgaacg ggctcaatta tcacacaggc agtgcggttg
    16441 gttattcggg caaggtctgc tatactgttg aattcaagta tcgaaatatc cgggagtaat
    16501 ggccgaaagg atcttttctg gctttcaacc cccatcacgc taagcgcacc ctgtgtgccg
    16561 ccatggtagg catttcggaa cgagattatt tctgtcctac cagtaaacct tttggatagc
    16621 ttcattgccc cctcaacagc ttcgcttcca gagttaacaa agtaaaccga gctaagcgtc
    16681 tggggtagca tggatgaaag tttagcggcc agtagtacct gagggctctg gatgaactcg
    16741 ccgtaaacca tcaggtgcat gtatgccgag gcctgatcct gaacagcttt gactactctg
    16801 gggttagcgt gtccaacatt gctcaccgag attcccgaaa tcaaatcgta gtatttttcg
    16861 ccacttggcc caaaaagaaa aaccccctcg gctctgctta cctcaagcat caggggtgca
    16921 tcggatgttt gggcaacatg gctaagaaaa agttgcctga tgttctgcat ataaaaggtt
    16981 acctgctatt acttgttgat cagattaatt tcgcttaaca gtttatcgcg gtatgacttt
    17041 ccgataggga ttattttacg tccctcagga gttcggatca ctgtagcgtt atcctcaatg
    17101 ctttcaattt tgccaacatt cactataaat gagcgatgaa tccttttaaa tcgaaccgaa
    17161 gaaagttgat tttcaatgga tttcatggtt agatgaatgg taaaccgctc gttaacggta
    17221 ttaataacaa tgtagttttc aagtgcctct atccatagta tgtcgctata ctttacccga
    17281 atcaacatgt tgtttttctt gatgaagatc tccttgtcat cgtcgtaggg agagcgttgc
    17341 tggttaaact tgtcgtaagc cttgctaaca gcttttgtaa agcggggaaa tgtaatgggt
    17401 ttcaggaggt agtcggtaac gctgtagtca aatgccttaa gggcgtactt ttccttcgag
    17461 gagattataa taacctgtgg ttcttgggag gtagcctcta aaaagtctat gccgctcatt
    17521 tcgggcatct ctatgtccag aaaaattaaa ttgactggtt caagtgaatt tttaagaaaa
    17581 ttaagggcat caactgcgtt gggaaaagag cccgttagcc gtatgaagtc aatttttgaa
    17641 gcataatctt caataactct gcgcgatagc tcatcatcat ctacctgtat gcagttcatc
    17701 ttgttctagt taagttaacc tgaaaaattc tttctaaaga acctacacaa acttaatgca
    17761 atttattata ctctgcaaca aatctttttt cgacaatgtg gcagtttttc acggattttc
    17821 ttaaccagtc gagtagcaca acctctttct catattccga cagggggtat gcggggtttg
    17881 cttttgcccg gtgaaccaat tcgtagaggg ttatggcaac agaaaccgat atgttgtagc
    17941 tttcggtgaa gccgtacatc ggaatggtga taaattcttc cgaaaggctc atggcctctt
    18001 cggacaatcc caagagttct gtgccaaaaa gaatggcaaa ttttcctttt tcggtatcga
    18061 aatcgcgaat gctgcacccc agtgaatggg gtgaggtggc aactatccgg tagccgctac
    18121 tcctcaatgt ttgtaacgcc tcttgggtta tgctcttatt gccggaatac tttttaatgg
    18181 tcagccattt tgatgcgccc atgtcaacct cggggttaac gttaaaagaa ttcctgtttt
    18241 cgatgatatg aacatcctga atgccaaggc attcacaact tcttagagct gcgctggcgt
    18301 tttgaggttg gtacaaatcc tctagaacca cagtaatgta gcgtgttcgt ctgctcaaaa
    18361 cctccctgag caagtttagc ctgcgttcaa gcaccaggtc agacaggtag ctggttagct
    18421 ggagacgaaa ctgccgatca ctctgtatta aatggtcaat gccgctcatt ccatactatt
    18481 atcattctta ctaagtgggt tatgcagttc agctcctggg gtgattaggt cttgtaacag
    18541 ctagcatcat ccgactgaat agggttaacg cccttcaacc aggttagctg atgggtaaaa
    18601 tttacccctt aagccgaatg gctaccatga ttaatttgcc tggctggaag cataacatgt
    18661 aacaaaaggc tagcttgcac ggctttacct tcaagtaaaa aaaaaggaga acactaaggt
    18721 tctcccttgg gattttctgt attagctatt gaacttttcc attcagttcg ctaccagctt
    18781 taaacttaac aaccttctta gccttgatgt tgatgggctt tccggtttga gggttacgtc
    18841 ctgtgcgggc gctcctcttg gcaacggaga atgagccaaa acctaccagg gcaactctat
    18901 cacctttctt aagggcggtg ccagtagttt tgatgaaagc atcaagtgct ttctttgcat
    18961 cagccttggt aaccttagct tcagctgcaa ttgcatcaat taattgagct ttgttcataa
    19021 tagataaatt ttagggttaa aaaatagttg ttagttggct ttccatgaca aaattattac
    19081 ataaatcagt aaaagcaagt tttttaaaaa aaaaatttgt catttcagcc ttttttgttg
    19141 gtttttaaga aaccaaaatt tttaccaacg ctaaattatt gaatttttaa acaatcatgc
    19201 aactattttt ataagaaaat tcgttttttt ttgattttct ccgcaaaatt tctaagtgaa
    19261 aaacgacatt aagtgttata atgtattata tatcaataac atacaattta tttcaggtat
    19321 ctacttgatt ttaaaaaaat tatcaccaag ttatcaacag ttgctatgtt gtaggctgtt
    19381 tttaccacta ttgcctcaaa gttttgccct aaatatgcct ggaattatat actttttccc
    19441 ttttttgctt tgcaggaata aaaaaaggcg gtacttttgc cgccggagaa atggcagagc
    19501 ggtcgaatgc ggcggtcttg aaaaccgttg ttcgccttac ggcgaaccgg gggttcgaat
    19561 ccctctttct ccgcagaagg
//
LOCUS       DAOSHM010000044        18962 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_2357_length_18962_cov_19.996086, whole genome shotgun
            sequence.
ACCESSION   DAOSHM010000044 DAOSHM010000000
VERSION     DAOSHM010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 18962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18962
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2357_length_18962_cov_19.996086"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            218..2092
                     /locus_tag="PK839_07625"
     CDS             218..2092
                     /locus_tag="PK839_07625"
                     /inference="COORDINATES: protein motif:HMM:NF024985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HOG72640.1"
                     /translation="MKKLLALFFLVSLIVGNAYTQELPEKNLKIQVEEVTVFIDGAQL
                     TGKATTELSKGKSLLKFTNLSPYIDAKSIRVKASNSVSVLAVNHQQNFVDRLQKPKKL
                     TDLEATLRTVAEGLKLENINLEINSEELKFMQDNRIIGGKNQETSVANFRETSSFYGT
                     KISALKLKELEINKKIEELNKKQNELQNQLNTLTSQKQYPSGEVMVNVEASNDVTATF
                     EVTFMVGNAGWFPSYDIRALSIDQPVVLVYKANIKQDSKTDWKNVKLKLSTSEPNVSG
                     VPPTLKTYFLNYNTLPPTYSQQLGRVTGRVMDSERNPLPGVSVTVTGTTISTVTAADG
                     SYSITLPASASYLTFSFVGYQSQTLPVNPIMNVVLSENNMMLDEVVVTAYGITAEKRK
                     LAAPSSKSADEATYMDEAGGVVGQQGIAQKPTAVDFEIREPYTVDSDNKNHTVEMVTY
                     SLPAMYEYYSVPKIDRSAFLLARITDWEKYSLLDGEANIFFENTFVGATVLDISRATD
                     TLQVSLGRDKGISINREKIKDYTTRQLVGTKKEETRTWRTTVKNNKLQKISITVMDQV
                     PVSTSEEIEVNILETSGGKVNTENGEVTWELTLEPGAEKKLDLKYSVKYPKNKVLVIE
                     "
     gene            complement(2187..2762)
                     /locus_tag="PK839_07630"
     CDS             complement(2187..2762)
                     /locus_tag="PK839_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005654735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72641.1"
                     /translation="MDKKLVFKDVLSNGVSTGLKNFLSLIGCVVLWLLTVWIPYINVG
                     TTIAIITLPAALSKGAVISPLEIFNKRYFRYMGEFFLVEGLAGIIIFSAMLFMFFPAI
                     VMSIAYSFGALLVVDKGKGASEALRLSNKATYGNKWTIFWVQLVLILPMFIFAWIWRP
                     LAIIYAILYVPFTLGATAFIYKELTGDISEE"
     gene            complement(3040..3594)
                     /gene="def"
                     /locus_tag="PK839_07635"
     CDS             complement(3040..3594)
                     /gene="def"
                     /locus_tag="PK839_07635"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010537701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOG72642.1"
                     /translation="MILPIYVYGSSILRKVAQDINPDYPNLKRLIDDMFETMSQSDGI
                     GLAAPQVGLSIRLIVIDGTPLAKDDPSLKDFRKVIINPYIEERFGEKVIYSEGCLSIP
                     NIHEEVEREEKVRISYLDENFTAHNEVFEGFAARIVQHEYDHLEGILFTDRLSPIRKQ
                     LLRSKLGAISKGKFTADYKCRLVK"
     gene            complement(3663..4085)
                     /gene="ruvX"
                     /locus_tag="PK839_07640"
     CDS             complement(3663..4085)
                     /gene="ruvX"
                     /locus_tag="PK839_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005836066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HOG72643.1"
                     /translation="MGRILALDIGQKRTGIAVTDPLKIIANGLTTIPSHTVLGFLADY
                     FSKESVELLVVGSPKQMDNSSSEAMKFIDPIVRGIRKTFPGMPIQFIDERFTSKMAKQ
                     AMIDGGMKKMDRRNKAMVDTISATIILQSYLESLSFNR"
     gene            complement(4085..4849)
                     /locus_tag="PK839_07645"
     CDS             complement(4085..4849)
                     /locus_tag="PK839_07645"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOG72644.1"
                     /translation="MLKALIVDDEIASFRTLEVLVGQFISNVQIVDIARSAAEALDKV
                     ERISPDLVFLDIEMPGGDGFDFLERCAARKFEVIFITAYNRYAIRAFRYSAIDYLLKP
                     IDVDELRAAVERAIKRRYANFDGHNKYYALFENLRSVIPSKLVVTANQVCEYINLNDV
                     CFLEDDDKMTRITFTGGNTMRVENQLSYFEEILGDRHFYRINSKQLVNVDHIRRIANR
                     ASDNLELDNGTLLAVDVGLKAGLERCLEKYIKLGKE"
     gene            complement(5038..8070)
                     /locus_tag="PK839_07650"
     CDS             complement(5038..8070)
                     /locus_tag="PK839_07650"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HOG72645.1"
                     /translation="MLSKIKNYLAKLHIPYFPFARYLKAGGSAIGFMLIGISGGHSQI
                     VSQPVTRHITVDEGLAASEVYHVFQDSRGYIWFGTNNGVSRFNGYEFENFDLVSGLPD
                     NTVFEIYEDYKHRIWFIPFSGSLSYYENGEIKSYRYNSKIVEHLPNSRGPSKLSFFVD
                     SLDNVSFGLKDFGIISIDQEGIYKHIDDSNTDAQVVVRSVGSGKFLVSDSHEPRKYGL
                     FYFDGRQSYRFTLSELGIKPFKPIYFYAQQAKDGSAFITIDGNIFRIKDGRVVSHNHS
                     ITQSVIWISIDADNNLWVSAFEGGVYMFPNCDLESPERLCFLKDYQVTSVLRDDEGSY
                     WFSTLNDGVFYVPDHQIAVFSKENGLSDNRVNAVYEKDGKIYVGFEMGFVDVIENGQI
                     THYKLSSKENIGITYVRDIYGDTLTGRVLVAAFGHVAYFENNKLKKLIPDSFNLNTRR
                     IIGSRGGGYWLATAKGFYKIENNRIAYDSRSDGFSGMVFSMAEDYKGDLWFNTVNGLW
                     RYSDSNLLFMGSENQLLAKIGHSMFFSHRDSSLWIGTNGAGIVIYGKDGKVSQISAAD
                     GLVSNSIHYLFPEGNRIWAATRQGLSILTLVNDTTYNIQNLTVLDGLPSNEVTTVFVM
                     DGIAYIGTNKGLAILNYSNLGFNHTPPKIEVLGVFVDGRPVAPVNGAVQINYSSNVVN
                     FSYIGLAYRNMGNILYRYRLMGLDSTWVFTRSTSCIFSNLKPGSYTFQVQAQNSNGIW
                     SSRSASIDLIIRPPFWERFWFIILAATILAGTVYAVYSLRISEIKRRNELINSINLYK
                     QQSLRQQMNPHFIFNTLNSIQYFILERDTVSSHKYLTKFARLMRLTLDNSLSPTVSLR
                     DELEALRLYLDLEALRLEGKFTYSIDYNDDESILNNKIPTLMIQPFVENAIWHGIMLK
                     KEKSGHVWVKIADEGSRIVCTIRDDGVGRTEAQRIQEQTGKEYSSRGFQITKQRIELL
                     NTLYGEKFSIKFIDLFDDMGNASGTLVSISIPKNLDVV"
     gene            complement(8329..11856)
                     /gene="nifJ"
                     /locus_tag="PK839_07655"
     CDS             complement(8329..11856)
                     /gene="nifJ"
                     /locus_tag="PK839_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HOG72646.1"
                     /translation="MAKSKKFITCDGNYAAAHIAYMFSEVAAIYPITPSSTMAEYVDE
                     WAAFGRKNIFGDTVRVVEMQSEAGAAGAVHGSLQSGALTSTFTASQGLLLMIPNMYKI
                     SGELLPGVFHVSARSIAAHALSIFGDHSDVMSTRQCGFAMLATSSVQEVMDLAGVAHL
                     SAIKSRIPFLHFFDGFRTSHEIQKIEVLENDDLAPLLDQKSLQEFRDRALNPEHPVTR
                     GTAQNPDIYFQAREASNKFYMDVPDIVEGYMQEITKLTGREYHPFSFYGDPNAENIII
                     AMGSVTDTIRETIDYLMAKGEKVGMISVHLYRPFSAKYFFKVLPSAVKRIAVLDRTKE
                     PGANGDPLYLDVKELFYGREKAPIVVGGRYGLSSKDTTPAQIIAVFDNLKLSEPKNQF
                     TIGIIDDVTFKSLPTGKEVNLAPKGTFQAKFFGLGSDGTVGANKNSIKIIGDNTPKYC
                     QAYFAYDSKKSGGITVSHLRFGDQPIRSPYLVITPDFVACHVQSYLGKYDVLKGLQPG
                     GTFLLNTIWSKDELNEKLPNSIKRYMAENSIKFYIINATQIAEEIGLGNRTNTIMQSA
                     FFKISGVIPYDVAVKEMKKAIEKSYGRKGEEVLRMNFAAVDRGGDAIQVVVPMEWAKL
                     QPENKLEIEGAPEFINKVVLPINLQMGDDLPVSAFVGREDGTFPAGTTRYEKRGIAVN
                     VPEWISENCTQCNQCAYVCPHAAIRPFLLTEEELKNAPAGTKTVRGNGGIKDFNFRVQ
                     VSVLDCTGCGNCVEVCPSKEKSLVMRPIESQMAEAARFDYMHEKVGYKDILGKEKSVK
                     NSQFAQPLFEFSGACAGCGETPYIKAITQLYGDRMIVANATGCSSIYGGSAPSTPYSA
                     GPDGRGVAWSNSLFEDNAEYGFGIATGVAKLRQRIAHKLQEALEGNFTSTTKEAMKEW
                     IAGKDNAFLSKAASAKLIAELSKESNPLAKELLSLKQYFEKKSIWIFGGDGWAYDIGY
                     GGLDHVLSTGEDVNVLVLDTEVYSNTGGQASKSTPAAAVAKFAASGKRVRKKDLGMMA
                     MSYGYVYVAQVAMGADHNQYLKAIKEAEAYPGPSLIIAYSPCINHGIRKGMGSTQSEA
                     KQAVKSGYWHLYRYNPLLEAQGQNPFQLDSKEPDWGMFQDFLKGEVRYTSLQLSFPDQ
                     ANELFKAAEENAKWRYNSYKKLAGVVEKP"
     gene            12165..13382
                     /locus_tag="PK839_07660"
     CDS             12165..13382
                     /locus_tag="PK839_07660"
                     /inference="COORDINATES: protein motif:HMM:NF017072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOG72647.1"
                     /translation="MVQRYKIGIVRETKTPPDRRVALPPNQARELTTRYSALEIAIQP
                     SSIRCFSDEEYARIGIKLQEDLSDCDLLIGIKETKIPTIIQGKTYMEFAHVAKKQPHN
                     RPLIQAFADQGNTLIDYEYLTDDKGNRLIAFGHWAGIVGAYNAIRALYIKEDLPELKP
                     AHQMHDYKEMQEVLKGLKVIPKKFVITGGGRVAGGAMEVLAQAGVKEVTPDEFLKGQF
                     DYPVYCRLDPWHYAKRKDGKPFDWSYWLSNPADHDTAFIPFTSATDILIACHYWDYRS
                     PHFFSREDMLNNSFRISIIADVSCDIPGPIPSTIKASTIADPFFDYNPKTDMEEPPFS
                     SKGNVTVMSIDNLPGELPRDASEFFGNILIEKIIPHIVTGDKEGIIERATILKNGKLT
                     GKFSYLSDYLKGE"
     gene            13587..14201
                     /locus_tag="PK839_07665"
     CDS             13587..14201
                     /locus_tag="PK839_07665"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOG72648.1"
                     /translation="MLRIAELICLFVLVPLAFYFDFIPLPKFVPLLVVFIYALVFLLR
                     SKTFSRKEFGLNGYRSWPGLILRVVAATIVIVVFSYYLLPDKFLIMPREQPLMWAMIM
                     IFYPVWSAFTQEVIYRTYFFHRYGAAIPNEKVLALVNGLLFGFLHIIFRNWVAVIGAT
                     LMGLLWAFSYLKHRSLLVVSIEHSIVGDMLYTFGLGYFFYVPDF"
     gene            complement(14331..14731)
                     /gene="ssrA"
                     /locus_tag="PK839_07670"
     tmRNA           complement(14331..14731)
                     /gene="ssrA"
                     /locus_tag="PK839_07670"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(14814..16673)
                     /gene="rho"
                     /locus_tag="PK839_07675"
     CDS             complement(14814..16673)
                     /gene="rho"
                     /locus_tag="PK839_07675"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005780404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HOG72649.1"
                     /translation="MYDILELNKKLLPELREIAKQLNIKKLESLKKQDLIYKILDQQA
                     INASMPGKGKEKERPSTSRPAVKLQPTEVVNKEQNAEAPLQQERSDSRDSAGKRVRFT
                     RNKSAEVKKEYSSTPARGEKVSKFSKQPDANELSIRTEQSNFPSSKEVFLPEVDLEPQ
                     TQREKKEDEVFADVLADDTQGIPEAEVSQSDSTPNIKIEEENFPLNPQESAVSNQLPE
                     PAGDGKHHPREHSGHKNVEKRNNERFDFDGIINSTGVLEIMPDGYGFLRSSDYNYLSS
                     PDDIYVSQSQIKLFGLKTGDTVTGTVRPPKEGEKYFPLIKVMEINGRNPEFIRDRVPF
                     DYLTPLFPNEKFTLIGNGMNLLSARIVDMFAPIGKGQRGLIVAQPKTGKTILLKDIAN
                     SIAANHPEVYMIVLLIDERPEEVTDMARSVNAEVISSTFDEPAERHVRVANIVLEKAK
                     RLVECGHDVVILLDSITRLARAFNTVQPASGKVLSGGVDANALQKPKRFFGAARNIEN
                     GGSLTILATALTDTGSKMDDVIFEEFKGTGNMELQLDRKLSNKRIFPSVDITASSTRR
                     EDLLLSKEVLQKIWILRNYLTDMNSVEAMEFLRDRLVKTATNEEFLWSMNSDI"
     gene            17157..18020
                     /locus_tag="PK839_07680"
     CDS             17157..18020
                     /locus_tag="PK839_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008625412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HOG72650.1"
                     /translation="MRQLKIIKQVTNRETVSLDKYLHEIGKVDLLSTDDEVALARKIK
                     EGDPEALSKLVKANLRFVVSVAKQYQNQGLSLPDLINEGNLGLIKAAQRFDETRGFKF
                     ISYAVWWIRQSILQALAEQARIIKLPLNKIGSINKVNRALVELEQKFEREPTINELSE
                     VLMLAPEDIKEALRSSGRPISMDAPLTQDEDSSMYDVILSPDSPQPDRGLLNESLRRE
                     IERALSTLTPRESSIIRMYFGLNGKQPLTLEEIGEELDLTRERVRQIKEKALRRLKQT
                     TRSKILKSYLG"
     gene            18120..18770
                     /gene="rpe"
                     /locus_tag="PK839_07685"
     CDS             18120..18770
                     /gene="rpe"
                     /locus_tag="PK839_07685"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006283450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOG72651.1"
                     /translation="MTHLVAPSLLSADFANLSADIEMVNNSLADWFHLDIMDGRFVPN
                     ISFGFPVVERIRDKASKPLDVHLMIVDPDRYLERFHDAGASWLTVHYEACTHLNRTLD
                     QIRKLGMKAGVALNPHTPVELLIDILDYTDLVLIMSVNPGFGGQKFIDSSLGRIERLR
                     AMIDKRGLSTLIEVDGGVDTNNASALYRAGANVLVAGNAVFKANKPLDVIAQLRKA"
     gene            complement(18767..>18962)
                     /locus_tag="PK839_07690"
     CDS             complement(18767..>18962)
                     /locus_tag="PK839_07690"
                     /inference="COORDINATES: protein motif:HMM:NF012991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72652.1"
                     /translation="REYVEPMSLAAVMAGADGLLLEVHQTPERALSDGQQTLNFEESH
                     RLFLKVQKLVALRERLEVAD"
BASE COUNT         5326 a         4345 c         4147 g         5144 t
ORIGIN      
        1 tttaaagcca gctgttgcac caatttagct ccgtataaat catataatat ggcttcacct
       61 aaaattttcc ccttcaccat cttgaaattc tttacgaaac ggctacgtgt tttacatcaa
      121 cccatgtaag cgatgaagta aaaattctca actaacgaag ttcagctaaa tcaattttta
      181 atcacctgat atttaacact aataataact tgaagcaatg aaaaaactac ttgccctatt
      241 ttttttagtc tccttgatag tgggaaatgc ctacacccag gaactcccgg agaaaaacct
      301 aaagatacag gttgaggagg ttacggtatt catagatggc gcccagctaa ccggaaaggc
      361 cactaccgag ctttcaaagg gtaaaagctt gctgaaattt acgaatcttt caccctatat
      421 tgatgcaaaa agcattcggg taaaggctag caatagtgta agcgtgctgg cggttaacca
      481 ccaacaaaat tttgtagata ggctgcagaa gccaaagaag ctaaccgacc tggaagctac
      541 ccttcggaca gtagcagagg gattgaaact tgaaaacatc aacctggaaa ttaactccga
      601 agagctgaag ttcatgcagg ataacaggat aattggcggc aaaaatcagg aaaccagcgt
      661 agctaacttt agagaaacat cctcattcta cggcactaaa atttcagctc tgaagttgaa
      721 ggaactcgaa attaacaaga agatagaaga gctgaataaa aagcaaaatg aacttcaaaa
      781 ccagctgaac accctcacca gccagaaaca gtatcctagt ggtgaagtga tggttaacgt
      841 tgaagcgtca aatgatgtaa cggctacttt tgaggtaacc ttcatggttg gaaatgcagg
      901 ttggtttccc tcttacgata tcagggcttt aagcatagac caaccagtcg tattggttta
      961 taaggcaaac ataaagcaag attcaaaaac cgattggaaa aatgtgaagt tgaagctatc
     1021 aacttccgag ccaaatgttt caggagtacc ccctacgcta aagacttact tcttaaacta
     1081 caacacccta ccgccaacct atagccagca acttggaagg gttacgggaa gggtaatgga
     1141 ttctgaacga aaccccctac cgggagtaag tgtaaccgta acaggaacaa caattagcac
     1201 cgtaactgca gctgacggaa gctactccat taccctgccg gcaagcgcaa gctacctgac
     1261 cttttccttc gttggctacc aatcgcaaac acttccggtt aaccctatca tgaatgtggt
     1321 gttgagcgag aataatatga tgcttgatga ggtggtagta accgcttacg gaataaccgc
     1381 cgaaaagcga aagcttgctg ctcccagctc aaaatcagcc gatgaagcaa cctatatgga
     1441 tgaagctggc ggagtggttg gccaacaggg aattgctcaa aaacctactg ccgttgattt
     1501 tgaaattcgt gaaccctaca cggtagactc ggacaacaaa aaccatacgg ttgaaatggt
     1561 tacctatagc ctgcccgcca tgtacgagta ctacagcgtt cccaaaattg acaggagtgc
     1621 attccttttg gccagaataa ccgactggga aaagtatagc ctgctcgatg gtgaggccaa
     1681 catatttttt gagaatactt ttgttggcgc tactgttctc gatatctcca gggcaacaga
     1741 cacccttcag gtatcacttg gtagggataa gggcattagc attaaccgcg aaaaaattaa
     1801 agactacacc acccgccagc tggtaggcac caaaaaggag gaaacacgaa catggcgcac
     1861 taccgttaaa aataataagc ttcagaaaat aagcataact gtgatggacc aggttccggt
     1921 atcaacctct gaggagattg aagtaaatat tctggaaacc tcgggaggca aggtaaatac
     1981 agaaaatgga gaggttacct gggagttgac gctggaaccg ggagcagaaa agaaactcga
     2041 cctgaagtat tccgtaaagt atccgaaaaa caaggttctg gttattgagt aggttctgtt
     2101 cataccttta agttgttgct aagaaaaacc ccttctcatc acgacggggt ttttcttttt
     2161 agctgcaaga atcttttaca gctatcctac tcctcactaa tatcaccagt aagctcctta
     2221 taaataaaag cagttgcgcc aagggtgaag ggcacataaa gtatggcata gataatggct
     2281 aacggtctcc aaatccaggc aaagatgaac atggggagga tgagtacgag ctgaacccag
     2341 aaaattgtcc acttgttccc gtaggtagcc ttgttgctaa gccgtaaggc ctccgaagcg
     2401 ccctttccct tgtcaactac cagaagagcg ccaaagctgt aagcaattga catcacaatg
     2461 gcagggaaaa acatgaaaag cattgccgaa aaaattataa tgcctgcaag cccctccacc
     2521 aggaagaatt cacccatgta cctaaaatac cttttattaa atatttcgag gggtgaaatg
     2581 actgcaccct tgctcagtgc tgcgggaagg gtgataatgg caatggttgt cccaacgttt
     2641 atgtagggaa tccaaacggt aagcaaccag agcaccacgc agccgattag cgaaagaaag
     2701 ttctttagcc ccgtgctaac accgttggat agcacatcct tgaatacaag tttcttgtcc
     2761 atagttgttt ggttttagtt tgaaataacc aaataaattt acaatcaact tgttaaataa
     2821 acaatactac ctgaatacaa aatttgttaa tcaaatagga atgcattaac aactcgggtt
     2881 ctgcaattga ttgcgtgagc atgtaaagga tattatccta caatagtgga tgtagattac
     2941 cgtcgaaata aaccgaaaaa aatatagaat atttgggcaa ccaccattgc cattatttac
     3001 aaattaaagt tgccagtaat cgatagattg caggcaacgc tacttaacca gacggcactt
     3061 ataatcggcg gtgaatttac ccttagatat tgcacccagc tttgatctca gcagctgctt
     3121 gcggataggc gaaaggcgat cggtaaagag gattccctcc aggtggtcgt actcgtgctg
     3181 gacaatgcga gcagcaaagc cttcaaaaac ttcgttgtga gcggtaaaat tttcgtccag
     3241 atagctaatc cgaactttct cctcacgctc tacctcttca tggatattcg gaatgctgag
     3301 gcatccctcg ctgtaaatca ccttttctcc aaaacgttcc tcaatgtatg ggttgataat
     3361 cacctttcta aaatccttta gggaggggtc atctttagct agcggagtac catcaattac
     3421 tatcaacctt atagataggc caacttgagg tgctgccagg ccaattccgt cggactggct
     3481 catggtttca aacatatcgt caatcaacct tttcaggtta gggtagtcag ggtttatgtc
     3541 ctgagccact tttctaagaa ttgacgagcc gtaaacgtaa ataggaagaa tcatagcaaa
     3601 taaataaagc tgtacatcat tttttaacaa gcctatttca aaaaagttta acctaagccc
     3661 tcctaacgat taaacgaaag gctttcgaga tacgactgaa gaataattgt tgcgctaatg
     3721 gtgtcaacca tagctttgtt gcggcgatcc atttttttca tgcccccatc aatcatggcc
     3781 tgctttgcca ttttagaggt aaagcgctca tcaataaact gaataggcat accaggaaaa
     3841 gtctttctaa tcccgcgcac aattggatcg ataaacttca tagcctcaga tgaagagtta
     3901 tccatctgtt tagggctacc aactaccaga agttcaacac tttccttact aaagtaatcg
     3961 gctaaaaaac cgagtaccgt atgggagggt atggtagtca acccgttggc aattatcttc
     4021 aaaggatctg ttaccgcaat tcccgttcgt ttttgcccaa tatcgagtgc taaaattcgt
     4081 cccactactc tttccccaat ttaatatatt tttccaggca cctctccagg cctgctttta
     4141 acccgacatc tacagctaac agtgttccat tgtccagctc caggttatcg cttgccctat
     4201 tggcaattcg tctgatgtgg tccacgttaa ccagctgctt tgaattaatt cgataaaaat
     4261 gacggtcgcc tagtatttct tcaaagtagc ttagctggtt ttcaaccctc atggtgttac
     4321 caccggtaaa ggttattcgg gtcattttat cgtcgtcttc caggaagcac acgtcgttta
     4381 ggttgatata ctcgcatacc tggttggctg taacaaccag ctttgatggg attaccgagc
     4441 gcaaattttc aaacagggcg tagtacttgt tgtgcccgtc gaagtttgca tagcgacgtt
     4501 taattgcacg ctcaacggca gctcttagct catcaacatc aatcggttta aggaggtaat
     4561 ctatagcgct gtaccgaaat gccctaatgg catagcggtt gtaggctgta ataaaaatta
     4621 cctcaaattt tctcgctgcg catctttcaa gaaaatcaaa cccatcgcca cccggcattt
     4681 cgatgtccag aaaaaccagg tcggggctta tcctctccac cttgtcaaga gcctcagcag
     4741 cagaacgcgc aatatcaaca atctgaacat tgctgataaa ctgccccaca agcacttcaa
     4801 gggtgcgaaa cgatgcaatt tcgtcatcaa caattagggc tttaagcatg agggcttgct
     4861 ttttgcaaag ttaaaaaaaa ttgggcaacc actaagcaaa acaaacgcat ggaagagaaa
     4921 acgagaatct ggtttaccca cagctatgga agtggctaac atgcagctca cttattataa
     4981 catgccgttt gcaacaatgc tgctcttcaa aaaaagacag cagctactga tgggtcgcta
     5041 aacaacatcc aggtttttag gaatggaaat acttaccaat gtcccactgg cattgcccat
     5101 gtcgtcgaag aggtcgataa actttatgct aaacttttcg ccgtaaaggg tattaagcaa
     5161 ttcgattcgc tgcttggtta tttgaaatcc tcttgaggag tattccttac cagtttgctc
     5221 ttgaattcgc tgggcttcgg tccggccaac accatcatcc cggatggtgc aaacaatcct
     5281 gctgccctca tcagcgattt tcacccacac atgtccgctc ttttcctttt taagcattat
     5341 accatgccaa attgcgtttt ccacaaaagg ctgaatcatc agggtgggaa ttttattgtt
     5401 aagtatcgac tcgtcgtcgt tgtagtcaat ggagtaggta aacttacctt cgagccgtag
     5461 ggcttccaaa tcgaggtaaa gcctaagggc ttctagctca tctcttagcg aaacagttgg
     5521 cgaaagagag ttgtcgagtg ttaagcgcat gagacgggca aattttgtaa ggtacttgtg
     5581 cgagctaact gtatccctct cgagtataaa gtactgtata gagttaaggg tgttgaaaat
     5641 gaaatggggg ttcatttgct gtcggagcga ttgctgcttg tagaggttga tgctgttgat
     5701 aagctcgtta cgtcgcttaa tttcgctgat tctcagcgag taaacagcat aaaccgttcc
     5761 ggcaaggatc gtggcggcaa gaattatgaa ccaaaacctc tcccagaaag gcggccttat
     5821 aatcaggtca atggatgcac ttcttgaact ccatataccg ttagaattct gagcctgaac
     5881 ctgaaaggtg tagcttccgg gtttcaagtt gctgaatatg cagctggtgc tacgggtaaa
     5941 tacccatgta gagtctagcc ccattaagcg gtaccggtat aatatattac ccatattcct
     6001 ataggccagc ccgatataag agaagttaac aacattactt gagtagttta tctgaactgc
     6061 tccatttaca ggtgctacag gacgtccatc aacaaaaacg cctaatactt cgatttttgg
     6121 tggggtatga ttgaatccca agttactata gttaagaata gccagccctt tgttggtccc
     6181 gatgtaagct attccgtcca ttacaaatac tgtggttacc tcgttgctgg gaagaccatc
     6241 cagcacggta aggttctgga tattataggt ggtatcgtta acaagtgtaa gaatggacaa
     6301 tccctggcgg gttgcagccc aaatcctatt cccttcggga aaaaggtagt gtatggagtt
     6361 ggaaaccaga ccatcagcag cagatatttg ggaaaccttg ccatcttttc catatatgac
     6421 tataccagca ccgttagttc caatccatag cgatgagtcg cggtgtgaga agaacatgct
     6481 gtggccaatt tttgccaaca gctgattttc tgaccccata aagagcaagt tgctgtcgga
     6541 atacctccaa agaccattta ctgtgttgaa ccacagatca cccttgtagt cctcggccat
     6601 gctgaacacc attccgctaa agccatcgct ccttgaatcg taggcaattc tattgttctc
     6661 aattttataa aaccccttgg cagtagccag ccagtagcca cctcccctac tgccaattat
     6721 ccgacgagta ttcaggttaa aactatccgg aataagtttt ttaagcttat tgttttcaaa
     6781 gtaggctacg tgcccaaatg cagctaccag caccctgccg gttagtgtat caccatatat
     6841 atccctgaca taggttatcc ctatgttttc tttgctgcta agcttgtagt gggtgatttg
     6901 tccgttctca atcacatcaa caaatcccat ctcaaaccca acgtagattt taccgtcctt
     6961 ttcatataca gcattaaccc ggttgtccga taagccattt tccttcgaga aaaccgcaat
     7021 ctggtggtcg ggaacataaa aaacaccatc gttaagggtg gagaaccagt aggacccctc
     7081 atcatccctg agcactgagg taacctgata gtctttaagg aagcatagcc gttccggcga
     7141 ttccaaatcg caattgggaa acatgtaaac ccctccttca aaggctgata cccacaggtt
     7201 gttatcagcg tcaatgctaa tccatattac cgattgcgta atggaatgat tatggcttac
     7261 aaccctgcca tcctttatcc taaaaatatt accgtcgatg gtgataaaag ccgagccatc
     7321 ctttgcttgc tgtgcataga agtaaatcgg cttaaacggt ttaatgccca gctcgcttaa
     7381 agtaaacctg tatgactgcc ttccatcaaa gtagaatagg ccatacttcc gaggttcatg
     7441 tgaatcggaa actaaaaatt ttcccgaccc tacgcttctg acaacaactt gggcatcggt
     7501 gttgctatcg tcgatatgct tgtaaatccc ctcctggtct attgaaatga ttccgaagtc
     7561 ctttagacca aaagaaacat tgtccaagct gtccacaaaa aatgacagct tagaagggcc
     7621 cctgctgttg ggaagatgct caacaatttt ggagttgtac ctatagctct ttatttctcc
     7681 gttttcatag tatgagaggg aacctgagaa cggtataaac catattctgt gcttataatc
     7741 ctcgtagatt tcaaaaaccg tattatcagg caaccccgat accaagtcga agttctcgaa
     7801 ctcatagccg ttaaaacggc taacgccgtt gttagttcca aaccagatat atccacggga
     7861 atcctgaaaa acatggtaaa cttcgcttgc ggcaaggcct tcatcaactg taatatgacg
     7921 tgttacaggc tgggacacga tttggctatg cccaccgctt atgcctatca acataaaccc
     7981 tatggcactt cctcctgctt taagatagcg ggcaaaagga aagtagggaa tatgcaactt
     8041 tgctaaataa ttttttatct tagacagcat gccacaggtt ttttgttgag taactttata
     8101 atcctcccag gttgatatct ttttgcgaat cgcaaaaaat tcctatcggc ttataaaagt
     8161 aaataaacat tgcatgaaaa aaaatttgcc catctaaata tggcaaattt cataatcact
     8221 tttgcaagtt attttaaacg taaaaaggct tccctgcaat caggaaagcc ttttacaaat
     8281 ggtctataag aaaatgtgat cagctaaaat accttctaca caatcaaatt aaggcttctc
     8341 aaccacgcca gcaagttttt tataggagtt gtacctccac ttagcattct cctcggcagc
     8401 tttaaacagc tcgttggctt gatcggggaa ggaaagttga agggaggtgt acctcacttc
     8461 gcccttcagg aaatcctgga acatacccca atcgggctct tttgaatcga gctggaatgg
     8521 gttttgtccc tgagcctcaa gtagggggtt gtagcggtac aggtgccagt aacccgactt
     8581 aacagcctgt ttcgcctcac tttgagttga acccattccc ttgcgaatac cgtggtttat
     8641 acacggcgaa taggcaatta taagcgatgg cccgggatat gcctcggctt ccttaatagc
     8701 cttaaggtac tggttgtgat cggcgcccat agccacctgc gcaacgtaaa catatccata
     8761 agacatggcc atcatgccga gatctttttt acgtacacgt ttacccgaag cggcaaactt
     8821 ggcaacagca gcagctggag ttgatttgga agcctgccct cctgtgttgg agtaaacctc
     8881 agtatcgaga accagcacat tgacatcctc acccgtagag agcacatggt ccagcccccc
     8941 gtaacctata tcgtaagccc atccgtcacc accgaaaatc cagattgatt ttttctcgaa
     9001 gtattgtttt aagctgagca gctcttttgc caacgggttg ctttccttgg aaagttcggc
     9061 aatcagcttg gctgaagcgg cttttgaaag gaaagcatta tccttaccgg caatccattc
     9121 cttcatagcc tccttcgtag tgctggtaaa attaccttct agtgcttcct gaagtttatg
     9181 ggctatgcgt tgacggagct tggccactcc ggttgcaatt ccaaaaccat actcggcatt
     9241 gtcctcaaac aatgagtttg accaagccac acctctgcca tcaggcccgg cagagtatgg
     9301 ggtagatgga gctgagccac cgtaaatgga ggaacaacct gtagcatttg ccacaatcat
     9361 gcggtcgcca tagagctggg taatagcctt tatgtaagga gtttcgccac aaccagcgca
     9421 agctccgcta aactcgaata atggctgggc aaattggctg ttctttaccg atttttcttt
     9481 accaagaata tccttgtagc ccaccttttc gtgcatgtaa tcgaacctgg ctgcctcggc
     9541 catttgtgat tcaattggtc gcataaccag cgacttttcc ttggaggggc aaacctcaac
     9601 acagttgccg cagccggtgc agtcgagaac gctcacctgt accctgaagt tgaaatcctt
     9661 aattccgcca tttcccctaa cagttttggt tcctgcaggt gcgtttttca actcttcctc
     9721 ggttaaaagg aagggacgaa tggctgcatg ggggcaaaca taagcgcact ggttgcactg
     9781 ggtgcagttt tccgaaatcc actcaggaac attaactgca attcccctct tttcgtaacg
     9841 ggttgtcccg gcagggaaag ttccatcctc acgaccaaca aatgcgctca cgggcaaatc
     9901 atcacccatt tgaaggttta ttgggagcac caccttattg ataaactcag gggcaccctc
     9961 aatttcaagt ttattctcag gctgaagttt tgcccattcc ataggcacta caacctgaat
    10021 agcatctccc ccacgatcaa cggcggcaaa attcatcctc aaaacctctt ctcccttacg
    10081 tccgtatgat ttctcaatgg cctttttcat ctccttaaca gccacatcgt aggggattac
    10141 acccgaaatt ttaaaaaatg cgctctgcat tatggtgttg gtgcggttac ccaaaccaat
    10201 ctcctcggca atctgagtag cattaattat gtagaactta atactatttt cagccatgta
    10261 acgcttaata gagtttggta gcttttcgtt cagctcatcc ttactccaga tagtgttgag
    10321 caggaatgtt ccaccgggct gcaaaccctt aagcacatca tacttaccaa ggtatgattg
    10381 aacgtggcag gcaacaaaat cgggagtaat taccaggtaa ggcgaacgga ttggctgatc
    10441 gccaaaacgc aggtgggaaa cggtgatgcc tccagatttc tttgaatcgt aggcaaagta
    10501 tgcctgacag tactttgggg tattatcacc aattatttta atggagttct tgttagcccc
    10561 aacggttcca tccgacccaa gtccgaaaaa tttggcctga aaggttcctt taggtgcaag
    10621 gttcacctct ttaccggttg gtaacgactt aaaggtaaca tcatcaatga tgccaatggt
    10681 aaattgattt ttaggctcgc taagcttgag gttatcaaaa actgcaatga tttgggctgg
    10741 tgtagtatcc tttgagctga gcccgtaacg gccacccaca actatcggag ctttctccct
    10801 cccatagaag agctctttta catcgaggta taacggatcg ccgttggccc cgggttcctt
    10861 ggttctatca agcactgcta tgcgtttcac agctgaaggc agcaccttaa agaagtattt
    10921 cgcagagaaa ggtctgtaca aatgaacgct tatcatccca accttttcac ctttagccat
    10981 gaggtagtca atagtttcac gaatggtgtc ggtaaccgaa cccatggcaa taatgatatt
    11041 ttcggcatta ggatcgccat agaatgaaaa tgggtggtac tcccgcccgg taagcttggt
    11101 aatctcctgc atgtagccct caacgatatc gggaacatcc atgtaaaatt tgttggaagc
    11161 ctccctggcc tgaaagtata tatcggggtt ttgagccgtg ccacgggtta cgggatgttc
    11221 ggggttcaat gccctgtcgc gaaactcctg aagtgatttt tggtcgagca atggagctag
    11281 gtcatcgttc tccagcacct cgattttctg aatttcatgg gaggttctaa acccatcgaa
    11341 gaaatgcagg aatggaattc tcgacttgat tgccgagaga tgggctactc cggccaggtc
    11401 cattacctcc tgaacgctgc tggtggccaa catggcaaac ccacactgac gggtgctcat
    11461 aacatcgcta tggtctccga aaattgaaag ggcgtgtgct gcaatgctgc gggcagaaac
    11521 gtgaaatacc cctggaagca gctctcccga aatcttatac atgttgggta tcatcaaaag
    11581 aagcccttgg gatgccgtaa atgtcgaagt taatgctccg gactgaagcg atccgtgaac
    11641 agcaccagca gctccagctt cgctttgcat ctcaacaacc ctaaccgtat caccaaaaat
    11701 gtttttcctc ccgaaggcgg cccattcatc aacgtactcg gccatggtag atgaaggagt
    11761 aatgggataa atggcggcaa cctcgctaaa catgtaggct atatgggctg cagcgtagtt
    11821 cccatcgcat gtaataaatt tctttgattt tgccatttta tttaaggtat taagaaaatt
    11881 agtgcttttc tgaaattttt tgcaaaggta atacaattaa aaaactataa ccaggtttta
    11941 taacacacct taaatatgat gcccgttgaa agtcagcatt accaacaaac tccgtttcat
    12001 ataaagtaaa atttaaaatc gaatagcacg attaccttac acttgtttac agaacgagaa
    12061 atacttcaga atttagtaag ctattattgg aaatttaatt acagtttgtt agattcgcag
    12121 gttcatgatt ttttttaaca ttttttcacc ttaaatcaaa tgctatggtt caacgatata
    12181 aaataggtat tgtaagagaa accaaaacgc cacctgacag aagagtagcg ctgccaccaa
    12241 atcaggccag ggagcttacc accaggtact cagcccttga aattgctatc caaccaagta
    12301 gcataaggtg cttctccgat gaggagtatg ccaggatagg cataaaactt caggaagatt
    12361 tatcggactg cgacctgctg attgggataa aagagacaaa aatacccacc attattcagg
    12421 gtaaaaccta catggagttt gcccatgtag ccaaaaagca gccccataac cgcccgttaa
    12481 tccaagcgtt tgccgatcag ggcaatacac taattgacta tgagtattta accgatgata
    12541 agggaaacag gcttattgcg tttgggcact gggccggtat tgttggagcc tataacgcca
    12601 ttagagcgct ctacattaaa gaagatctcc cggaactaaa gccagcccac cagatgcacg
    12661 actacaagga gatgcaagaa gtgctgaagg ggttgaaggt catccccaaa aaatttgtca
    12721 taaccggcgg ggggcgagtt gctggcggcg ctatggaggt actggcacaa gccggggtaa
    12781 aagaagttac ccccgatgaa tttctgaagg gtcagttcga ctaccctgtt tactgtcggt
    12841 tagacccatg gcactatgca aaacgtaagg atggaaagcc tttcgactgg agctactggt
    12901 tgagtaaccc ggccgaccat gacacggctt ttataccctt tacaagtgca accgacatat
    12961 tgattgcctg ccactactgg gactaccgtt ctccccattt tttttcgcgt gaggatatgc
    13021 taaacaatag ctttcgcatt tcgattatcg ccgatgtaag ctgcgatatt cccgggccga
    13081 tcccatcaac cattaaagcc tcaacgattg ccgatccctt ttttgattac aaccctaaaa
    13141 ccgacatgga ggagcctccc ttctcgtcca aaggcaatgt aacggttatg tcgatcgaca
    13201 accttcccgg agagctaccc agggatgcca gcgaattttt cgggaatatc ctgatagaaa
    13261 aaattattcc acatatcgtt accggtgaca aggaaggaat aatagaacgt gctaccattc
    13321 ttaaaaatgg aaaactaacc ggaaaatttt cctacctatc cgattacctg aaaggagaat
    13381 aacctctaaa aaagggtact caaagctgtt ggacagttta tctttattaa gacagggcat
    13441 attcgggctt ttgggccaag gaatggtagt tcggtgtggg gtgttactta gagctaaact
    13501 agcataattg aacaacacta agcctaaaat aaacttttgg caggctgagc tgttaaattt
    13561 aatcctcaag ttggatagtt tttcctatgc ttcgtattgc tgagcttata tgtttgtttg
    13621 tgctggttcc actggcattc tacttcgatt ttatcccact gccaaaattt gttcccctgc
    13681 tggtagtctt tatttatgct ctagtattcc tgcttaggtc taaaaccttc agccgaaagg
    13741 agtttgggct taatgggtac cgtagctggc ctgggttgat actgcgtgtt gtggcagcaa
    13801 ccattgtaat cgttgtattc agctactacc tgttgcccga caaattcctc ataatgccgc
    13861 gtgagcagcc tctaatgtgg gctatgatta tgatttttta ccctgtttgg tctgctttta
    13921 cccaggaggt gatttacaga acatactttt ttcaccggta cggggcagct ataccaaatg
    13981 aaaaagttct ggcgttggtg aatgggttgc tattcggttt tcttcatatc attttccgaa
    14041 actgggtggc agtaatcgga gctacgctta tggggctgct gtgggctttc agctacctga
    14101 agcatcgctc gttacttgtt gtttctattg agcattcaat agttggcgat atgctctaca
    14161 ccttcggact tggatatttc ttctatgttc ccgatttctg aaagcctctc tccaaaataa
    14221 aaaaaatgga aagtataaca aagacttctg gagtaagggt atattcttga aataaaaaaa
    14281 gcgctccaaa ttataacatt tgagcgcttt gggccatcat acgggcatgc tggtggagct
    14341 ggagggaatc gaaccctcgt ccaaacaagg aaccagcttg ttttctacat gcttagcctt
    14401 tcttggttgt cgggactagc caggcgaaag gcaaccgaac caatccttaa cctttattat
    14461 tttacccaag cttaaaggtg tcgcttgagc tatcccggtt ttgtcggtgc cccgtatgcc
    14521 ggaaacaatc gggcggaatt tccggcggga catctcgtcc ccacacacct aggcgtaggg
    14581 attaagccat tttgctttgc aaaattaagc agcgagagcg taatttgatt cgccgtttat
    14641 ttggttgtga cctgtgatta tcgagccagc atcacgacgc tcggcatgct tacaaaccca
    14701 ttctacttgc tgtcaaaacc acgtcagccc caatggggtt ttagcaacgg caaagataca
    14761 ataaaaaacc gccggatatc aacattcggc ggttaaagtt tgcaaaaatg aagctatata
    14821 tcgctattca tcgaccagag gaactcctcg ttggtggctg ttttaaccag cctgtccctg
    14881 agaaactcca tggcttccac cgagttcatg tcggtgaggt agttgcgaag aatccaaatt
    14941 ttttggagaa cctccttact taacagcagg tcttcgcggc gggtgctgga tgccgtaatg
    15001 tcaaccgagg ggaatattcg cttgttggat agcttgcggt cgagctggag ctccatgttg
    15061 ccggttccct taaactcctc aaaaattaca tcatccatct tgctgccggt atcggtaaga
    15121 gcagtggcca aaatggtaag cgaaccgccg ttttcaatgt ttcgagctgc gccgaagaaa
    15181 cgctttggtt tctgcagggc attggcatca acaccacccg agagcacctt gcccgatgcg
    15241 ggctgaacgg tgttgaacgc gcgtgccagg cgggtaatcg agtcgagcag gattactaca
    15301 tcgtgaccgc actccactag ccttttagcc ttttcaagaa ctatgttggc cacccttacg
    15361 tgccgttcgg caggctcgtc gaaggttgat gaaattacct cggcgttgac gctacgcgcc
    15421 atatcggtta cctcctcggg tcgttcgtca atcagcagca caatcatgta aacctccgga
    15481 tggttggcgg caatggagtt ggcaatatcc ttcaacagga tagttttacc ggttttgggc
    15541 tgggcaacaa taagcccccg ctgacccttg ccaatagggg caaacatgtc aactatacgc
    15601 gccgaaagca ggttcatccc gtttcctata agcgtaaact tctcgttggg aaagagcggg
    15661 gttaggtagt cgaagggtac cctgtcgcgt atgaattcgg ggttacgccc gttgatttcc
    15721 atcaccttga taaggggaaa gtacttttcg ccctcttttg gaggacgaac ggtacccgtt
    15781 accgtatcgc cagtcttgag cccaaacagt ttaatttgcg actgtgaaac gtaaatgtcg
    15841 tcgggagagc taagatagtt gtagtccgat gagcgaagaa acccataccc atcgggcatt
    15901 atttcaagca cccctgtact gttgattata ccatcaaagt cgaatctttc attgtttctt
    15961 ttttcaacat tcttatgccc cgaatgctca cgggggtgat gcttgccatc accggcaggt
    16021 tcgggtagct ggttgctaac agccgattcc tgcgggttaa gaggaaaatt ttcttcctcg
    16081 atcttgatat taggagtgct gtcggattgg ctcacttcag cttcaggtat tccttgcgtg
    16141 tcgtcagcaa gtacatcggc aaaaacctca tcttcctttt tttctctttg ggtttgaggc
    16201 tccaggtcaa cttcaggaag aaaaacttct ttacttgagg gaaaattgga ttgctcggtt
    16261 cttatagata attcattcgc atcaggctgt tttgaaaatt tggacacctt ctctcctctt
    16321 gccggagtag aagagtactc tttttttact tctgcgcttt tgtttcgggt aaaccgcact
    16381 cgtttccccg cgctatccct gctgtctgat ctttcctgct gtagtggggc ttcagcattc
    16441 tgctccttgt tcactacttc ggtaggctgg agctttacag ccggtcggga agtgcttggg
    16501 cgctcctttt ccttgccctt ccctggcatt gaagcattga tagcctgctg atccaaaatt
    16561 ttataaatca agtcttgctt tttcaggctt tcaagctttt tgatatttag ctgcttggca
    16621 atttcccgca gttcgggaag gagcttcttg ttgagttcga gaatatcgta cataaaaata
    16681 gtttagttgt aaaatgaagg ttttagagta gttaaagaaa aattggaaaa aatactttgc
    16741 ataggcttgt aaaatggtaa tcaatgggca agccttttgc ttacatccta gtgtaatgct
    16801 taatataaat acgttaacat attgtaaagc tgtcaggtaa ataactttag atgaaattac
    16861 tcagtgatga aatagttgtt gaagtgaaat tgaagaaaga gggttggagt tctcttcccg
    16921 gttatttttt gcaaaggtat gaaatattat ttttcacctg caagaatttt atgatgattt
    16981 tttgttgaat ataaaataaa aaccattata gaacatggca ttgactatta aaaaaatggc
    17041 attagatgga aaccgccacc aaaaggttta aaaaaaatta cataaaaaaa tcgaagcatt
    17101 aacaaaattt aataattgta attatattca cccccaaaca aagcatcgtt attggaatga
    17161 ggcagctaaa aataatcaag caggttacca accgtgaaac ggtatcgctc gataagtatc
    17221 tgcacgaaat tggcaaggtg gatttgctta gtacagacga tgaggtggca cttgcccgaa
    17281 aaattaaaga gggtgatcct gaagcgttat ccaagctggt aaaagccaac ctcagatttg
    17341 tagtatcggt tgccaagcag taccagaacc agggtttaag cctacccgac ctgataaacg
    17401 agggtaatct gggacttatt aaagcagctc agcgctttga cgaaacgagg ggatttaaat
    17461 ttatatccta cgccgtttgg tggattaggc agtctattct gcaggcgctg gccgagcagg
    17521 caagaattat aaagctacct ttgaacaaaa ttggctcaat taataaggtg aatagggcgc
    17581 tggttgagct ggagcaaaag tttgaacggg aacccaccat taatgagctg tccgaggtgc
    17641 taatgcttgc ccccgaggat atcaaggagg ccctgcggag cagcggccgt cctatttcca
    17701 tggatgctcc actgacgcag gatgaagata gtagcatgta cgatgttatc ctttcgcccg
    17761 attctcccca acccgacagg ggattactta atgaaagttt aagacgagaa atagaacgtg
    17821 ccctatcaac cctcacgcct cgtgagtcca gtatcataag aatgtacttc ggtctaaacg
    17881 gaaagcaacc cttaaccctc gaggaaattg gcgaggagct cgaccttacc cgcgaacggg
    17941 tacgccagat taaggagaaa gcgcttcgaa ggctcaagca aaccactcgt agcaaaattc
    18001 tcaagtcata cctgggttag ccccgcttag cccttccgtt tttctctcca acatgcctta
    18061 aagcattcgg ggcaacacct agtattttaa attgctacct ttgcaaaaac cgattgctta
    18121 tgacccatct tgttgctcca tcgctgctct cggcagattt tgcaaatctt tctgctgata
    18181 ttgaaatggt taacaatagc ctggcagatt ggtttcacct ggatatcatg gatggcaggt
    18241 ttgtgccaaa tatatcattt ggatttccgg tagtagagcg aatcagggat aaagccagta
    18301 aacccctgga tgtccacctg atgattgttg accccgacag gtacctggag cgattccatg
    18361 atgccggtgc cagctggctt accgtacact acgaggcttg cactcattta aaccggaccc
    18421 ttgaccagat tcgaaaattg ggcatgaagg ccggagttgc tctcaacccc cacactcctg
    18481 ttgagctgct gatagacatc ctggattaca ccgatttggt gctgataatg tcggtaaatc
    18541 cggggttcgg tggacaaaag ttcatagata gctccctagg caggattgag cgactgagag
    18601 caatgataga taagcggggg ctaagcaccc tgattgaggt tgatggaggc gttgatacta
    18661 acaatgcatc ggctctatac agagccggag ccaacgtgct ggtggccggg aatgctgtgt
    18721 ttaaagccaa taaacccctg gatgtaattg ctcagctcag gaaggcttag tccgccacct
    18781 ccaacctttc ccgaagggct acaagctttt gaacttttaa gaagagacgg tggctttctt
    18841 caaaattcag tgtttgctgc ccatcggata gcgctctttc gggtgtttga tgaacttcaa
    18901 gtagtagccc atccgcccct gccatcactg ctgccaacga cataggctct acatattccc
    18961 ta
//
LOCUS       DAOSHM010000045        18554 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_2437_length_18554_cov_20.703065, whole genome shotgun
            sequence.
ACCESSION   DAOSHM010000045 DAOSHM010000000
VERSION     DAOSHM010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 18554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18554
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2437_length_18554_cov_20.703065"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(133..2214)
                     /locus_tag="PK839_07695"
     CDS             complement(133..2214)
                     /locus_tag="PK839_07695"
                     /inference="COORDINATES: protein motif:HMM:NF015926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF349 domain-containing protein"
                     /protein_id="HOG72653.1"
                     /translation="MSLENNNEITMSTNDLTDPKDQNLANSEGTMDSSSLAEQENEPV
                     ALERIEISNQLGDEPQGIHFDASTTVKSEGESAEATNIPVNEIELPAESEELEEVEDI
                     EDGDEEVDEESEEIKIDSVDFSSLTKDEMVQTLKKLINEKPIQKIKAEVESIKSIFYR
                     KHKLEVERLRREFVEAGGDVAGFTPPEDDAEIQIKELLKLYREKRAQYNGELEVQKKQ
                     NLELKMQIIEQIKDLVNSSEAVNVTFQEFRNLQQKFREVGPVPQANLNDLWENYHHHV
                     QNFYDYIKINKELRDLDLKKNLEEKIKLCEKAEELLLEPSVVAAFKKLQKYHTQWREV
                     GPVPHENQNEIWERFKEATAKINKKHQEYFESLRDEQKRNLDAKAALCEKAEELASLV
                     IKTNKEWNQRSKEMVELQKVWKTIGFAPKKDNNKVYERFRAACDTFFNNKRDFYSETK
                     EDQLNNLQLKTELCIQAESLKDSTEWKKTTEDFINLQKRWKEIGPVPRKNSDEIWKRF
                     RAACDYFFTQKSSHFSQVDNSYDKNLKDKEMLIAEIAAYQLTDNPEADLLALKEFQRR
                     WAEVGFVPIKNKEDIQKRYREAINKHFETLNIGEEKRNMFNFKTKLESATGNPRQENK
                     LRQERDKFFNRLRQLEGDIALWENNIGFFTRSKNAEAMIKEVERKIEKAKEEIRILEQ
                     KIKMIDSMDSK"
     gene            complement(2446..2895)
                     /locus_tag="PK839_07700"
     CDS             complement(2446..2895)
                     /locus_tag="PK839_07700"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOG72654.1"
                     /translation="MTLVTFNSPLTSGRPSGTSVVESIFDALRDTAMNLAEVSASYPA
                     VNIFEEPERFRIEVAAPGYKKEQFNIQVDANDVLTLSVNEGKNNVEGESYLRREFANG
                     SFERHFTLGEMIDANKIEATYRDGILTIVIAKKEEAKPQKPRTISIS"
     gene            3179..3856
                     /gene="trmD"
                     /locus_tag="PK839_07705"
     CDS             3179..3856
                     /gene="trmD"
                     /locus_tag="PK839_07705"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015546976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HOG72655.1"
                     /translation="MRIDIITVLPELLESPLGHSIIRRAQKKGLVEIYLHQLRDYTTD
                     KHRTTDDYAFGPDAGMVLKVEPIFKVIEKLKGERTYDEVIYTSPDGEPYTQGLANQLS
                     TMQNIILLCGHYKGVDHRVREHLVTREISIGDYVLSGGELAAAVIVDSVVRLVPGVLT
                     DETSALTDSFQDGLLAPPVYTRPAEFNGWKVPEILISGNFKEIEKWQLEQSIERTRRL
                     RPNLLGE"
     gene            complement(3878..5620)
                     /locus_tag="PK839_07710"
     CDS             complement(3878..5620)
                     /locus_tag="PK839_07710"
                     /inference="COORDINATES: protein motif:HMM:NF019500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HOG72656.1"
                     /translation="MSYSFESYRVEITDRQFTRSDGYTTPKTARVVLLNDRLSEIATL
                     NLAYPNLADVYEGIGRGETINLDCCLVQSFSATACKRYLGLDKSSLIPLLGLSAQNAF
                     FESGISIDFSLVDFRGDVNFNNSYMVAPLVDFHQSTHSNGNFSLSDCHIRTEHFNFTQ
                     CRFYGSNMSFKNTTFSPGEKDFQDCVFNSNDTLFTNTDFGNGNVSFINVKFGSSEINF
                     KVARFGTGKVDFHYANFGRGDVSFERTEFGPGRVDFRTVDFGTGRVSFNRSVFGDGEV
                     NFEGAEVKAEKLTFKRASFGTGNLIFDLFQGDTSDLVFERTIFSTNLSFHGVRLNNLL
                     MEDCQFNSMLNLHVEKCASISLPGCTIRDIAEFYTHGSKPMIDMLNISGVKLLGKLYI
                     DDEACNLKQLIYNQTDTTCTDKAEQFRILKENFNDLGRYKEEDRSYVEFKRCEAKARL
                     QRLKSKGFLHKIAGYVEYAAKKLIFDYMGLYATAPQRVIVSILVVYSLYSLSYALIML
                     MNIGIVEATGPSGAHLGLVAKGFYFSVVTFFTIGYGDFAPDGFARIIAGTEGFMGVFM
                     MSYFTVAFVRKILR"
     gene            complement(5806..6030)
                     /locus_tag="PK839_07715"
     CDS             complement(5806..6030)
                     /locus_tag="PK839_07715"
                     /inference="COORDINATES: protein motif:HMM:NF039796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6485 family protein"
                     /protein_id="HOG72657.1"
                     /translation="MDCKKESNSVGCRCTYPCTRHGVCCDCLMYHRKKGQLPACYFPK
                     DVETGYNRSIANFTRVISERGTGFLMDDSE"
     gene            6208..8259
                     /locus_tag="PK839_07720"
     CDS             6208..8259
                     /locus_tag="PK839_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72658.1"
                     /translation="MRKLITPLILLTMLIFNQCKTTSNEVSQDVSPISDKTIGKVTTE
                     LVAKYGEAQKFRIERGVGQTALLWRSSDGSEADFEEFCGKQFISNPEELDQLFNKLSY
                     GYEMLFGHFILLSKELMRPLHLDWGPVAPVDELFGSYNAASHVTDDLYGNKIAFITTL
                     NFPYYNLKEKAEMGEKWSRKEWAYARMGDMFTSRVPAQLIQDFSTANTNADTYISSYN
                     IYMGSLVNDEGKKLFPNDMALITHWGLRDELKSQYSNLVDGLEHQRIIYQVMKRIIDQ
                     SIPLQVINKGDYQWNPNSNTLQRNGEDIAVEPEPNTRYQHVINQFNTLRAIDAYTPAM
                     PTYIQRKFEGEYELPVDDVEKLFIELVSSHEVREAGKLISQRLGRKLEPFDIWYDGFK
                     VRSGISAEKLDGLTTKKYPTVEAFDKALPEILTSLGFPKDKAQFIGDHVRVEAARGSG
                     HAWGAEMKKDDALLRTRVPATGMNYKGFNIAMHEFGHTVEQTISLHDVDYYTMHGVPN
                     TAFTEALAFVFQNRDLAVLGISETNPQKESLETLDNLWSCYEIMGVSLVDINLWKWLY
                     NHPGASAQEVKDATINIAKDIWNKYYADVFGSTDEPILAVYSHMIDNPLYLSAYPVGH
                     LIHFQLEKQLKGKSFASEIERIYSQGRLIPQLWLKDAVGETLSVKPILEASAEALGKL
                     K"
     gene            8305..8493
                     /locus_tag="PK839_07725"
     CDS             8305..8493
                     /locus_tag="PK839_07725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72659.1"
                     /translation="MPVRSFWLLLLARCKFLHTPVREEVLANGRGVAGGCKSGKSHKQ
                     GQRADAQKTDRRLTSDLL"
     gene            complement(9297..10232)
                     /locus_tag="PK839_07730"
     CDS             complement(9297..10232)
                     /locus_tag="PK839_07730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HOG72660.1"
                     /translation="MDTSVKVLSVNVSDAKGTAKHPVDFIELNSTGVKGDAHSGMWHR
                     QVSLLGVESIEKFNAANNLEIKYGDFAENITTKGFCLYECIPGDRLVGSGVELEVTQI
                     GKLCHADGCAIFSQVGSCIMPREGIFARVLKTGRLKAGDELELIPRTYKVKVVTLSDR
                     ASRGDYEDRSGPEVVRLVNQFFNDIGWRHSVNSTIIPDDAATLTSTVVEAKNNGYDLL
                     ITTGGTGIGPRDVAPDVVRPLLDKEIPGIMEMIRMKYGADKPNALISRSIAGVMGSMF
                     VYTLPGSVKAVGEYMQEIIKIQKHLFLMLMGIDSH"
     gene            complement(10244..10699)
                     /gene="moaC"
                     /locus_tag="PK839_07735"
     CDS             complement(10244..10699)
                     /gene="moaC"
                     /locus_tag="PK839_07735"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001612668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HOG72661.1"
                     /translation="MELSHTDRDGRANMVDVGSKPNQLREARAKGFIRLQPETLKLIT
                     ENQVKKGDVLTLAEIAGIQAAKQTPTLIPLCHTILLAKVDVKASIVQNGIEISSYIKC
                     IGQTGVEMEALTAVSVALLAVYDMCKAVDKEMTIESIKLTEKIKHDINS"
     gene            complement(10701..11501)
                     /locus_tag="PK839_07740"
     CDS             complement(10701..11501)
                     /locus_tag="PK839_07740"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOG72662.1"
                     /translation="MYDRFNRQINYLRISVTDRCNLRCTYCMPEEGITQLDHSEILSF
                     EEIASFTRIAATKGFNKVRITGGEPLVRKGVVELVSMISNIEGITDLSMTTNGVLLGK
                     YAKELKYAGLQRVNVSLDTVNPTRYRQITRVGSLDDVLNGLECARAAGLTPIKINCVV
                     DKSSAEPDAVEVKKFAQANGFEVRFIPKMDLNAGIFGEVEGGNGGNCSICNRLRLTAN
                     GFVKPCLFSNLGYSVRGLGYEGALLKAVNAKPARGTTNHDGKFYNIGG"
     gene            complement(11485..12666)
                     /locus_tag="PK839_07745"
     CDS             complement(11485..12666)
                     /locus_tag="PK839_07745"
                     /inference="COORDINATES: protein motif:HMM:NF015418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HOG72663.1"
                     /translation="MIRFEEAYALVLSRVVTIQKPERVLLLESLHRVLAEDIVSDINM
                     PPFRKSAVDGFACRMSDLRMPLKIVETIAAGSLPTMQVEPGTCAKIMTGAPIPLGADC
                     VLPVEDTRLADDLYVEFTGMEAKKNICELGEDIRIGDVSINKGIIIGPQHIAIMAALG
                     YHQPLVATQPRVGILPTGDELVEPNIKPLGGQIRNSNGYQLVAQTKAAGALPSYYGIV
                     SDSKDATYEVLAKAIAENEVVALTGGVSMGDYDYVPEIMEKLGVTIHFSSVAVQPGKP
                     TTFGTLDNKLVFGLPGNPVSAFIQFELLVKPAIRKLMGCKQLNPTAIRLPLAEDYRRN
                     RAERMALVPVKIIAGSAVLPVEFHGSAHIFALGNADGIIAVPIGTTKIDKGEIVDVRP
                     L"
     gene            12822..13379
                     /locus_tag="PK839_07750"
     CDS             12822..13379
                     /locus_tag="PK839_07750"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOG72664.1"
                     /translation="MLKDRGYYIDLLHQHISNPKMIAHCLASEAVLRALARKFGEDEE
                     VWGIAGLVHDIDSEQTESDHTMHGLVAAEMLNGVLPDEAVDAVRMHNEDATGMERSTK
                     FQHALAAGETITGLIFATALVYPDRKISSVKPKSVVKRMKEKQFAASVNRSTIMECEL
                     LGLSIDDFVSLSLEALAPIESELGF"
     gene            13628..14434
                     /locus_tag="PK839_07755"
     CDS             13628..14434
                     /locus_tag="PK839_07755"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HOG72665.1"
                     /translation="MKLELICEQVVTEAKKAGSFAMEQRANFNINKVEVKGKHNFVSY
                     VDRTCEAMLVDGLSKIVPQAGFIAEEGTGKPNKGGLNWVVDPLDGTTNYIHGISPFAV
                     SIALMDDQKLLLGVVYEANLKECFWATADSQAYLNGAPIKVSETGKVNDALVATGFPY
                     YDYSRINPFFQTMEYFMRNSHGLRRLGSAATDLVYVACGRFDAFYEYGLSPWDVAAGA
                     LIVERAGGRVCDFKRGNDFIFGGEIMATNSSLYGEFADILNGMMSKSQSE"
     gene            14516..15430
                     /locus_tag="PK839_07760"
     CDS             14516..15430
                     /locus_tag="PK839_07760"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOG72666.1"
                     /translation="MKRLAFYLFYTITKILALLPLRLLYVISDINRFLLYYVLRYRRK
                     TVTTNLKNAFPDKSKEELKAIEWKFYRHLCDLFAEIIFLLHSGAKRAAKLCTFSNVEL
                     VNSYYQQGKSVVAAGGHFGNWELYGLFPLYIKHTVLGAYKPLNNKPIDEFIKKSRERF
                     GCVTIPMYSISRVALKWSQEGKPFFLGLINDQSPSRSHIGYWTTFLNQETPVYIGTEK
                     IARKINQPVVFCYIRKLKRGKYEANVELLVEEPRLTKPHEITEMHLRALERVINEAPE
                     YWLWSHKRWKHKRDVTTNSLQAGNEHTP"
     gene            15414..16442
                     /locus_tag="PK839_07765"
     CDS             15414..16442
                     /locus_tag="PK839_07765"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018108313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOG72667.1"
                     /translation="MSIHHEVAVVILNWNGEKFLQRFLPGVLANSNETGVAVYVADNG
                     STDGSVKLLRSRFPLVNLILLDRNYGYAGGYNRALSQIDSDFFVLLNSDVEVPGGWLN
                     PLRSFMRSNPNAAACAPAIMDIARPECFEYAGAAGGFIDKFGYPFCRGRILSEIEQDT
                     GQYNQSIEVFWASGACMMVRANTFWDAGGFDDHFFAHMEEIDLCWRMLNMGYSVWSIP
                     ESKVYHVGGGTLPNNNPYKLYLNYRNSLFMLHKNLSKNAFYQTIIARLLLDWLSAMAY
                     LVRFQLKFFGAVAKSHYHYYRDISRLNSFRSSFRHPKTNSAKVIYHRSVLFDFFILRR
                     RKFSELKF"
     gene            16594..18279
                     /locus_tag="PK839_07770"
     CDS             16594..18279
                     /locus_tag="PK839_07770"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOG72668.1"
                     /translation="MISFKKLTIRDILFRSRMAYAERPALAFVDSKPVTYANMAIIVR
                     NISGMLYTLGIRKGDRVALIGENSPNWGMAYLAATTIGAVVVPILPDFSDTELENIIR
                     HSEARVVFVSAKIYSHLKIDNLKGVEAFILLNSLVPIDEETFTADTTIPYSIPDPIVN
                     VDYCDCELPRPSEDDLAAIIYTSGTTGRPKGVMLTHRNLVSNVLSTLMVQEVTEHDRL
                     LSILPLSHTYECTIGFLIPMATGASVYYLDKPPVAQTLIPAMQKVRPTMVLTVPLIIE
                     KIFRGSIKPQLTSTALRQRLYQFSLSRRLLHRVAARKLHSTFGGELRFFGIGGAKLSR
                     EVELFLHEGRFPYSIGYGMTETSPLLTGSTPAQVRFRTAGYCLPGQELRLLNPNPETG
                     EGEILARGSNVMVGYYKEPELTQAIFYEGWLRTGDLGVIDDKGYLTIKGRSKNLILSS
                     SGENIYPEEIEDIINNHEYVADSVVYESGGKLMARVHLNREAVAQYLAGIKSSAADLS
                     KNAEELTRRILDDIKVMVNSKVARFARLSVVHEQLEPFEKSATQKIKRFMYQD"
BASE COUNT         4766 a         4248 c         4188 g         5352 t
ORIGIN      
        1 gcctccgggc ggaacttgtc cgcctactgg cggattcaaa atttcatttt caaacttttt
       61 acctttaacc aaaactactt ctacagttca cagctaataa ctgttttgca tggttgaaat
      121 gcaccgcttt tgctacttgc tgtccataga gtcgatcatc ttgatttttt gttccaaaat
      181 ccgaatttcc tccttagctt tttcaatttt acgttcaacc tccttaatca ttgcctcggc
      241 attttttgaa cgggtaaaga acccgatgtt gttttcccat agagcaatgt caccctccag
      301 ctggcgcagg cggttgaaga acttatcgcg ctcctgccgg agcttatttt cctgtctggg
      361 gtttcctgtt gcgctttcta gcttggtttt aaagttgaac atgttgcgct tttcctcgcc
      421 aatattaagc gtttcgaagt gcttgttgat agcctcgcgg tatcgtttct gaatgtcctc
      481 cttatttttt attggcacaa agccaacctc ggcccagcgg cgctgaaatt cctttaaggc
      541 cagcaggtcg gcttccggat tgtcggttag ctggtaagcg gcaatttcgg caataagcat
      601 ctccttatcc ttcaggttct tatcatagct gttatcaacc tgggagaagt ggctggactt
      661 ttgggtgaaa aagtagtcgc aagctgcacg gaagcgtttc caaatctcat ccgaattttt
      721 acgtggaacg ggtccaatct ccttccagcg cttttgcagg ttgataaagt cctcggtggt
      781 ttttttccac tccgtgctat ccttcaggct ttcggcctgt atgcaaagtt cggttttaag
      841 ctgcaggtta tttagctgat cctctttggt ttcgctgtaa aaatcgcgct tattattaaa
      901 aaaagtgtcg catgccgccc tgaacctttc gtaaaccttg ttgttgtcct ttttaggcgc
      961 aaagccgatg gtcttccaaa ccttttgcag ctccaccatc tccttcgagc gctggttcca
     1021 ctccttgttg gttttgataa ccagcgaggc aagttcttcg gccttttcgc aaagggcagc
     1081 tttggcgtca aggtttcttt tttgctcatc cctgagcgat tcgaagtact cctgatgttt
     1141 cttgttaatt ttagcggttg cctccttaaa cctttcccaa atctcgttct gattttcatg
     1201 tggaaccggg ccaacttccc gccactgggt atggtacttt tgcagttttt tgaaagctgc
     1261 tacaaccgag ggttcgagca ggagctcctc ggctttttcg caaagcttaa tcttctcctc
     1321 gaggttcttt tttaggtcaa ggtcgcgtag ctccttgttt atttttatgt agtcgtagaa
     1381 gttttgcaca tgatggtggt aattttccca cagatcgttc aggtttgctt gcggaactgg
     1441 cccaacttcc cggaactttt gctgaagatt tctaaactcc tgaaaggtaa cgttaaccgc
     1501 ttcggagctg ttcactaaat cctttatttg ctcaataatt tgcatcttaa gctcaaggtt
     1561 ctgtttcttc tgaacctcca gctcgccgtt gtattgtgcc cgcttttcgc ggtatagctt
     1621 gagcagttct tttatttgaa tttccgcatc atcttccggt ggtgtaaaac ccgccacatc
     1681 accgccggct tcaacaaact cgcgtcgtaa acgctcaacc tccagcttat gcttacggta
     1741 gaatatgctc tttatgcttt caacctcagc cttgattttt tggatgggct tttcgtttat
     1801 gagttttttg agggtctgaa ccatctcatc ctttgtgagg ctcgaaaaat ctacactgtc
     1861 aattttaatt tcctcacttt cctcatcaac ctcttcatca ccatcttcga tatcttctac
     1921 ctcttccagc tcctcgcttt cggcgggtag ttcaatctca ttaacgggga tgttggttgc
     1981 ttcagcagac tccccttcgg attttacggt tgtacttgca tcgaaatgga taccctgggg
     2041 ttcatccccc agctgattgg aaatctcgat acgctctagc gcaacaggct cgttttcctg
     2101 ctcggctaaa ctggaagaat ccatcgtacc ctcgctattt gcaaggtttt gatccttagg
     2161 atcagtaaga tcgttggtgc tcatggtaat ctcgttatta ttttctaaag acatacaaac
     2221 gtatggctgg gtttaacgtg atttgcgaaa atatgcaatt catcccatcc tcaaattatt
     2281 ttcgtcaaaa gtattgattt ttgaggatta cccaaaattg gtataaaatt acaggttgac
     2341 ttttatgcaa aatgcttccc aatgcagtag ggaaagcagg aacaaaaaaa cctaatggag
     2401 aaaacgccat taggtttatg tgttgctatt tgtatcaact tgcggttagc tgatgcttat
     2461 ggttctgggt ttttggggtt ttgcctcttc ctttttggcg atgacgatgg tcaggatgcc
     2521 atcacggtag gtggcttcaa ttttatttgc atcaatcatt tcgcctagcg taaaatggcg
     2581 ttcaaatgaa ccattggcaa actcacgacg caagtacgat tctccttcaa cattgttttt
     2641 cccctcattc acgctaaggg taagcacgtc gttagcgtca acctggatat tgaactgttc
     2701 ctttttatag cctggtgcag caacctcaat acggaacctc tcgggttcct cgaaaatatt
     2761 cactgccgga tagctggcgc tcacttctgc caggttcatg gccgtatctc taagggcgtc
     2821 gaatatgctt tcaactaccg atgttccact tggcctccca gaggttaatg gactgttaaa
     2881 agttaccagt gtcatagctt ttctgtttta ttgtgttgat ttaacgtttc ttaatctact
     2941 tccaacttta tgccaaacga agcaatcggc catattgacg tataatgaag tccgaatatg
     3001 tcataatggc gttttgcgcg tcatggtgtg gttttgctga tggttagctt actgttgaga
     3061 aacagagaac agttttacta ccggatttgg aatgccgtgg taaggctaca acgtgccttg
     3121 ccctggggta gcattacacg gtaaaaaacc gtaaatttgc ggttgtaaaa aggcagccat
     3181 gcgaatagat ataattaccg ttttgcccga acttctcgaa agtcctttag ggcattcgat
     3241 aataagaagg gcgcagaaaa aggggttggt ggagatatac cttcaccagc taagggacta
     3301 caccaccgat aagcatcgaa ctaccgatga ttacgccttt ggacccgatg cagggatggt
     3361 gctgaaggtg gagccaatat ttaaggttat tgagaagcta aagggcgaac gtacctacga
     3421 tgaggttatc tacacatcgc ccgatggcga accctatacg cagggactag ccaaccagct
     3481 aagcaccatg cagaacataa ttctactttg tgggcactat aagggagttg accaccgcgt
     3541 tagggagcat ttggttacgc gggaaatttc aattggcgac tacgtgcttt cgggaggtga
     3601 gctggctgct gcggttattg tagatagcgt ggtgaggctt gtgccaggtg tgctaaccga
     3661 cgaaacctct gctttaaccg attcgtttca ggatgggctg cttgcacctc cggtttacac
     3721 ccggcctgcc gaatttaacg gttggaaagt gcccgaaatt cttatttccg gaaactttaa
     3781 ggagattgaa aagtggcagc tggaacaatc catcgagcga acccgtaggc ttcgtccaaa
     3841 cttacttggt gagtgaatat tgctttagtc gttttagcta cctaagaatt ttcctaacaa
     3901 aagccaccgt aaagtacgac atcataaaaa cccccataaa cccttcggtg ccggcgataa
     3961 ttcgtgcaaa tccatcggga gcaaaatcgc catagccaat ggtgaagaaa gtaacaacgc
     4021 tgaagtagaa accttttgca accaacccaa ggtgggcacc cgaaggacct gttgcctcaa
     4081 caattcctat gttcatgagc attatcagcg cataggatag gctgtaaagc gaatatacaa
     4141 ccagaatgct tacgataacc cgctgaggag cagtggcata tagccccatg taatcgaata
     4201 ttaacttttt tgcggcatac tcaacataac cagcaatttt atgaagaaag cccttgcttt
     4261 tgagcctttg gagccttgcc ttagcttcgc atcgtttaaa ctccacgtac gacctgtctt
     4321 cctccttata cctgccaaga tcgttaaagt tttctttaag aattctaaac tgttcagctt
     4381 tgtcggtaca ggtggtatcg gtttggttgt atatcagctg tttaaggttg caggcctcgt
     4441 cgtcaatgta gagttttccc agcagcttga ccccggaaat gttcagcatg tcaatcattg
     4501 gtttgctgcc gtgggtgtag aactctgcaa tgtctctgat tgtacaacca ggaaggctga
     4561 ttgaagcaca tttttccaca tgaaggttga gcatggagtt aaactggcaa tcctccatta
     4621 ataggttgtt taaccttacg ccgtgaaaac tcaagttagt gctgaaaatt gttcgttcaa
     4681 ataccagatc ggaagtatca ccctggaaca aatcaaatat caggttaccg gtgccaaagg
     4741 atgcccgctt gaaggttagc ttttcagctt taacctcggc accctcaaag ttcacctcac
     4801 catcgccaaa tacagaccgg ttgaacgata cccttccggt gccaaaatct acggtgcgga
     4861 aatcaaccct tccgggtccg aattcggtac gctcaaacga aacatcaccc cttccgaagt
     4921 tggcatagtg aaaatcaacc ttaccggttc caaaccgggc taccttaaag tttatttcgc
     4981 tgctgccaaa tttaacgttg ataaaggata catttccatt gccaaagtcg gtattggtga
     5041 aaagggtatc gttgctgtta aaaacgcaat cctgaaagtc cttctccccc gggctaaagg
     5101 tggtattctt aaacgacata ttgctgccat aaaacctgca ttgggtaaag ttgaaatgct
     5161 cggttcggat gtggcaatcg gataggctaa agttgccatt tgaatgggtt gactggtgaa
     5221 aatcaaccag aggggcaacc atataggagt tgttaaagtt cacatcacct cgaaaatcaa
     5281 ccaacgaaaa gtcaatgctg atgcctgatt caaaaaatgc attttgggcc gacaatccca
     5341 aaaggggtat cagggaactt ttatccaggc ctaggtatcg cttacatgca gtagctgaaa
     5401 aactttgaac caagcagcag tcaaggttga tggtttcacc cctgccaata ccttcgtaaa
     5461 catccgccaa attaggatat gccaggttaa gcgtggctat ctccgataac ctgtcgttaa
     5521 ggagcactac acgcgcagtt ttaggggtgg tgtaaccgtc ggagcgggta aactgtcggt
     5581 cggtaatttc tacccgatac gattcaaaac tataggacat tcaggatgtt gtagttcatg
     5641 taaaaatatg aaaaatatcc aaattttgac aggctttaga atatttttta aaaggaaaat
     5701 gatattactc cttggccctg ttatgccttt gccctttctt atctatacat caatgttcag
     5761 cgtgtaggtt ttgaatagct atgttgagat gatcttgcat aggttttact ctgaatcatc
     5821 catcagaaat cctgttccgc gctcgcttat tacccttgta aagttagcaa tggagcgatt
     5881 gtagccagtt tccacatctt ttggaaagta acaggctgga agttgaccct ttttccggtg
     5941 gtacatcagg caatcacagc atacaccatg cctggtacag gggtatgtgc agcggcaacc
     6001 taccgagttg gattctttct tgcaatccat cgttttttaa ctacaaatat aggctgaaag
     6061 gattatcatc attcaaaaac atcagtattt ttatgatgaa ggacttgtgg attaggttag
     6121 ctttaaactt aatctttact ttgaagtcga aataatgaaa tgctttttgc atcgtgtaat
     6181 tcatgttcaa cctaaaaacc ttatgccatg agaaagttga ttaccccatt aattttattg
     6241 acaatgctta tattcaacca gtgtaaaacc acaagtaatg aggttagcca ggacgtttcg
     6301 cctatcagtg ataaaaccat aggcaaagtt accaccgagc tggtagcaaa gtatggcgaa
     6361 gcccaaaagt ttagaattga gaggggcgtt ggccaaaccg cattactttg gcgtagctcc
     6421 gacggaagtg aggccgattt tgaggaattt tgcggcaagc agttcatttc caaccccgag
     6481 gagcttgacc agctattcaa caagctttca tatggatacg agatgctgtt tgggcatttc
     6541 atactactta gcaaggagct gatgcgtccg cttcaccttg attgggggcc tgttgcccct
     6601 gttgatgagt tgtttggttc ctataatgct gcctcgcatg ttaccgatga tctttacggg
     6661 aacaaaattg cctttattac cacccttaac tttccatact acaacctgaa ggaaaaagct
     6721 gaaatgggcg agaagtggtc gcggaaggaa tgggcttacg cacgaatggg cgacatgttt
     6781 acaagtaggg ttcccgccca gctgatacag gacttttcca ccgccaatac caatgccgat
     6841 acctacatca gcagctacaa tatctacatg ggaagtctgg taaacgatga aggcaagaag
     6901 ttatttccca atgatatggc actaattact cactggggtt tgcgtgatga gctcaaatcg
     6961 cagtactcca acttagtcga tggacttgag caccagcgga taatttacca ggtgatgaag
     7021 cgaattattg accagtcaat tcctttacag gtgataaata aaggcgacta ccagtggaat
     7081 cccaactcga ataccctaca aaggaatggt gaggatatag cggttgaacc cgagccaaac
     7141 acccgttacc agcatgttat taaccagttt aacacgctta gggctattga tgcctacact
     7201 cctgcaatgc ccacttacat acagcggaag tttgaaggag agtatgagct accggttgac
     7261 gatgtggaga agctgtttat agagcttgtt tcgtcgcatg aggtgcggga ggctggcaag
     7321 ctcatcagcc aaaggctggg aaggaagctc gaaccctttg atatctggta cgatgggttt
     7381 aaggtacgga gcggcatttc tgccgagaag ttggatggac ttacaaccaa aaagtatcct
     7441 actgttgagg catttgacaa ggccctacct gaaattctta cctcgttagg ctttccaaaa
     7501 gataaggcgc agtttattgg cgaccatgtt cgggtcgaag cagcaagagg atcaggccat
     7561 gcatggggtg ccgagatgaa gaaggatgac gctttgcttc gaacccgtgt ccctgcaaca
     7621 ggcatgaact acaagggatt taacattgcc atgcacgagt ttggacacac tgtggagcaa
     7681 accatttcgc tccacgatgt tgactactac acaatgcacg gtgtccccaa cactgctttc
     7741 accgaagcct tagcctttgt ttttcagaac cgcgacctgg ccgttctggg aatatcggaa
     7801 accaatcccc agaaggaaag cctcgaaacc ctcgataacc tttggtcgtg ctacgaaata
     7861 atgggtgttt cgctggtgga tataaacctt tggaagtggc tctacaacca tcccggtgcc
     7921 tccgctcaag aggtaaagga tgccaccatc aacattgcca aggatatttg gaacaagtac
     7981 tatgccgatg tgtttggcag caccgacgag cctattttgg cggtttactc ccacatgatc
     8041 gacaacccac tttacctgtc ggcgtaccca gttggacatt taattcactt tcagctcgaa
     8101 aagcagctaa aaggcaagtc gtttgctagc gagattgagc gtatatactc ccagggaaga
     8161 cttattcccc agctttggct gaaggatgcg gttggtgaaa ccctatcggt taagccaatt
     8221 ctggaggcct ccgctgaagc cttaggtaag ttgaagtaat ccggctaaga cctgaaattt
     8281 tttatacgac ctaacaggct ttcaatgcct gttaggtctt tttggttact actactggca
     8341 agatgcaagt tcttgcacac gccggttagg gaggaggtgc tagcaaatgg caggggtgtt
     8401 gcgggtggct gcaaatctgg aaaaagccat aagcaagggc agcgtgcaga cgcacagaaa
     8461 accgatagaa ggctgacaag cgacttgttg taaaaaatcc tctcggattc aggttgtatt
     8521 tttttcgaaa ctattgcaaa ttgattccta aattcatctc acagatgtaa ttaaaattgt
     8581 tgtccgtttt gcattccttt caacgaagca ccgtcatttt agaattaccg ccaactataa
     8641 acttgttttg gtgacgtcgg tcctgctgta gataggctag tatagcgtag gcgtttatgc
     8701 tatcaacggt aatgcatagc ttgccatctt tagatttccg tatgatttta tcttcatgtc
     8761 aaaattaacc tattgcttaa ttcttatcaa ttttttattg gtatgattaa tgttttttat
     8821 aaaaataagt aataattaac tgattagcaa cgatatggat tgttgataaa tttatggttc
     8881 tgccgccttt ataaccgttt tgtcttctaa cctgtaatct ttttcatttt ggccatattc
     8941 aaaaataatg ttgtaatacc gattatcatt ctatatgata aatttattaa acttttcttt
     9001 gagaactttg tggttaaata atattagaaa ccacaaagca cacaatgata ttacacaaag
     9061 aacgcaataa ttactattca tgattccaat atggctatcg gaaaacaaca ttttctttaa
     9121 atagagcctc cctttttatg ttctccccca acccctgcaa tcctagcctt tatcttttgc
     9181 tcaaatatca caattagctc tttgatggcg ttaatcaatt cctcctcttt ttcaacttga
     9241 gaaacgattg tcttttgaac gtttaaccta cggtttatac atcattgttg acttcactag
     9301 tggctgtcta ttcccatgag catcaggaac aggtgtttct gaattttgat gatttcctgc
     9361 atgtactcac caactgcctt aacgcttcca ggcagggtat atacaaacat acttcccatc
     9421 accccggcaa tactcctgct gattaaagcg ttgggcttgt cggctccgta tttcattctt
     9481 atcatctcca ttattccggg aatttcctta tcaagaagtg gcctaaccac atcgggggca
     9541 acatcccttg gtcctatacc cgttcccccg gtagtaatca ggaggtcgta cccattgttc
     9601 ttggcttcaa ccacagttga ggtaagcgta gcggcatcat ccggaattat ggtggaatta
     9661 acggagtgcc tccatcctat atcgttgaag aattggttaa ccaaccgaac cacctcgggg
     9721 ccgcttctat cctcatagtc acccctgctg gccctgtcgc ttagcgtaac cactttcacc
     9781 ttgtaggttc gtggtatgag ctccagctcg tcaccggctt ttagccttcc ggtttttaag
     9841 accctggcaa aaattccctc ccttggcata atgcagctac caacctggct gaatatggcg
     9901 caaccatcgg catggcaaag ctttcctatt tgggtaacct ccagctcaac gcccgaccca
     9961 acaagcctgt cgccgggaat acactcgtaa aggcagaaac ccttagttgt gatgttctcg
    10021 gcaaagtcgc catattttat ttccaggttg ttggccgcat taaatttttc gatgctctca
    10081 acaccgagaa ggctgacctg ccggtgccac attccggaat gggcatcgcc tttaacccct
    10141 gtgctgttta gctcaataaa gtcaacaggg tgctttgctg tgccctttgc atccgaaaca
    10201 ttaaccgata gtacttttac gctggtatcc acaacttttt gctttacgaa ttaatgtcat
    10261 gttttatttt ttcggtgagt tttatggact caattgtcat ttccttgtca acggctttgc
    10321 acatgtcgta aacagccaac agggcaactg aaacggctgt gagggcttcc atttcaacac
    10381 ctgtttggcc gatgcactta atgtagctgc ttatttcaat cccgttttga acgatggatg
    10441 cttttacatc aaccttagct agcagtatgg tgtggcaaag cgggatgagc gttggcgttt
    10501 gtttggcagc ctgtattccg gcaatctcgg caagggtgag cacatcaccc ttttttacct
    10561 gattctctgt gataagcttt agggtttcgg gttgaagcct gataaatcct ttggcgcgtg
    10621 cttccctaag ctggttgggc ttggagccaa cgtcaaccat gttggccctg ccatctctgt
    10681 cggtatgtga aagttccatg tcaacctcct atgttataga atttcccgtc atggttggta
    10741 gttccacggg caggtttggc attcactgct ttgagtaagg ctccctcata tccaagcccc
    10801 cgtacactat agcccagatt actaaacaga catggtttga caaaaccgtt ggctgttaac
    10861 ctgaggcgat tgcagatgct gcagttaccg ccgtttcccc cctctacctc gccgaatatt
    10921 ccggcgttta aatccatttt aggaatgaac ctgacttcaa atccatttgc ttgggcaaat
    10981 ttttttacct caacggcgtc gggttctgcc gatgatttat caactacaca gtttattttt
    11041 attggggtta ggcctgcagc tctggcacat tccaacccat taagcacatc gtcaaggcta
    11101 cctaccctgg taatttgcct gtacctggtg gggttgaccg tatcgaggct aacattaact
    11161 ctctgtaaac cagcatattt taactcctta gcatatttgc ccagaagcac tccgttggtg
    11221 gtcatgctca ggtcggtgat gccctcaatg ttggaaatca tggacacgag ttccactaca
    11281 ccttttcgaa caaggggttc accgcctgta attctcacct tgttgaaccc ttttgtggct
    11341 gcaattcttg taaaggatgc aatttcctca aacgagagta tctcgctatg gtcaagctga
    11401 gtaataccct cttcgggcat gcagtaagtg caccgtaggt tacaacggtc ggttaccgat
    11461 atccgcaggt agttgatttg gcggttaaag cggtcgtaca tcaacaattt ctcccttatc
    11521 aatttttgtg gtaccaatag ggacagcaat aatgccatcg gcattgccaa gggcaaatat
    11581 gtgtgccgag ccgtgaaact caacaggtaa aactgctgac cctgctatta tcttaaccgg
    11641 aaccagcgcc attcgctcgg ccctgtttcg cctgtaatcc tctgctaaag gcaaacgaat
    11701 ggccgtaggg ttcagctgct tgcagcccat tagcttacgt atggcgggct taaccagtag
    11761 ctcgaactgt atgaatgccg aaacaggatt acctggcagc ccaaatacca gcttgttgtc
    11821 caaggttccg aacgttgtag gcttgccggg ctgaactgct acgctgctga agtggatggt
    11881 aacccccaac ttctccatga tttcgggcac gtagtcgtaa tcgcccattg aaacgccacc
    11941 tgttagcgca acaacctcgt tttcggcaat ggctttggct agaacttcgt aggtagcatc
    12001 cttgctatca ctcacaatgc catagtagct gggcaaagct ccggcggcct tagtctgtgc
    12061 aaccagctga tagccattgc tgttccttat ctgcccaccc agtggtttta tattaggctc
    12121 caccagctca tcgccggtag gtagtatgcc cactctgggc tgagtggcaa ccaatggctg
    12181 gtggtatccc agtgctgcca taatggcaat atgctgtgga ccaataatta tgcctttatt
    12241 gatagataca tctccaatcc gaatatcctc acccagctcg caaatatttt ttttggcctc
    12301 catgcctgtg aattcaacgt acaaatcgtc tgccaggcgg gtgtcctcaa caggtaaaac
    12361 gcagtcggcg ccaagcggaa ttggtgcccc agtcattatt ttggcgcaag taccgggttc
    12421 aacctgcatg gtgggcaggc ttccggcagc aatggtttct actatcttta agggcattcg
    12481 gaggtcgctc atcctacagg caaagccatc aacagccgat tttctgaaag gcggcatgtt
    12541 aatgtccgaa actatatcct cagcaagcac cctgtgcagg ctctccaaca gtaaaactct
    12601 ttctggcttt tgaatggtaa caactctgga gagaaccaag gcgtaagctt cctcaaacct
    12661 aatcatggtc agggttaatt ttccacaaag ttgttaaaaa aatcattacc agggtaactg
    12721 ttttaggctt ttttgcctgc tcatccgtaa tagcctgctt aattaataaa gcgctggtta
    12781 gctattgcta aaattgttaa ttttgacaaa aattttgtag gatgttgaag gatcgcggtt
    12841 actatatcga cctgcttcac cagcatattt caaaccccaa aatgatagct cattgtctag
    12901 cctctgaggc agtgctaaga gccttagcca gaaagtttgg tgaggatgag gaagtttggg
    12961 gaattgccgg tttggttcat gatattgact ctgagcaaac cgagtcggac cacaccatgc
    13021 atgggctggt ggctgccgag atgctaaacg gcgtgctacc cgatgaggcg gtagatgctg
    13081 ttcggatgca caacgaggat gctaccggaa tggagcgtag caccaagttc caacatgcgc
    13141 tggcagccgg tgaaaccatt accgggctaa tttttgccac cgcgctggtt taccccgaca
    13201 ggaagattag cagcgttaag cctaagtcgg ttgtcaaaag aatgaaggag aagcagtttg
    13261 ccgcctctgt gaatcgtagc actatcatgg agtgtgaatt gttagggctt tcgattgacg
    13321 atttcgtcag cctttcgctg gaggcacttg ctccaattga gtcggagttg ggcttttaga
    13381 gtttatcagg catgaaggct tgctgtttta ataactggag aaaatttttt cagaatttcc
    13441 tcctgaatac ctttaaagtt ttggaaagct gcatgctgat caaattgatg ctggtgaatt
    13501 acatagctaa aatacggact aatttgcatt aatcatctta gctatgcgct tgcatgctat
    13561 atgatatgta tgcaggttga aagttatttt tttgttggca taaacactta ttaagctgaa
    13621 tattaacgtg aagttagagt tgatttgtga acaggttgtt accgaagcca aaaaggcggg
    13681 tagctttgcc atggagcaac gggcaaactt caacatcaat aaggttgagg tgaaaggcaa
    13741 gcataatttt gtatcgtatg ttgaccggac atgcgaggcg atgctggttg atggtctctc
    13801 gaaaattgtc cctcaagccg gctttattgc tgaggaggga accggtaagc caaataaagg
    13861 ggggctgaac tgggtggtgg atcccctgga tgggacaacc aactacattc atggaatatc
    13921 cccttttgcg gtcagtattg ccctgatgga cgatcaaaaa ctgttgctgg gcgttgtgta
    13981 tgaggccaac ttaaaggaat gcttctgggc aactgccgac agccaggcct atttaaatgg
    14041 tgcacctatt aaagtgtcgg aaaccggtaa ggtgaacgac gcccttgttg caaccggttt
    14101 tccctactac gactactccc gcatcaaccc gttttttcag actatggaat acttcatgcg
    14161 taattcccat ggtttgagac ggttaggctc tgctgctacc gatttggttt acgtggcctg
    14221 cggacgattc gatgccttct acgagtatgg gctaagccct tgggatgtgg ctgccggagc
    14281 tttgattgtg gagcgtgccg gaggtagggt atgcgacttt aagcggggta atgattttat
    14341 ttttggaggt gaaataatgg ctaccaacag cagcctatat ggtgagtttg ccgacatctt
    14401 aaacggcatg atgagcaaat cccaatcgga ataagttaaa catctatgaa agaagctcaa
    14461 ctattacagc caccggcttc atccgcgtca atcataaatt tttaaaaatt gacgaatgaa
    14521 acgactagct ttttacctgt tttacaccat aacaaaaatt ttagccctgc taccgctaag
    14581 attgctctac gtaatttccg acattaacag gtttttgctt tactacgtgc tgaggtacag
    14641 gcgaaaaacg gtgacaacta atttaaaaaa tgcctttccg gacaaatcga aagaggagct
    14701 aaaggcaatt gaatggaagt tttaccggca tctgtgcgac ttatttgccg aaataatttt
    14761 tttactgcat tcgggggcaa agcgggcagc taagctttgc acctttagta acgtggagct
    14821 ggtgaatagc tactaccagc aggggaaaag tgtggtagcg gccggtggtc atttcgggaa
    14881 ctgggagctt tacggcctgt ttccgctcta cataaaacac actgttctgg gtgcgtacaa
    14941 gccacttaat aacaagccga ttgatgaatt catcaaaaag tcacgtgaac gttttgggtg
    15001 tgttaccata ccaatgtaca gcatttcgag agttgcccta aaatggtcgc aggaaggtaa
    15061 acccttcttt ttggggctta taaacgacca atcgccatct cgaagccata ttgggtactg
    15121 gactactttt ttgaaccagg agactccagt ttatattggg accgaaaaga tagcccggaa
    15181 aataaaccaa cccgtggtgt tttgttacat tcgcaagtta aagcgaggca agtatgaagc
    15241 gaacgtggag cttctggtcg aagaacctag gctgactaaa ccccacgaga ttaccgagat
    15301 gcacctccga gcgttggaaa gggtgattaa tgaagctcct gagtactggc tctggtctca
    15361 taagcgatgg aagcataaac gtgatgtaac cactaactcc ttacaggcag gaaatgagca
    15421 tacaccatga ggttgccgta gttatcctga actggaatgg cgagaagttc ctgcaacgct
    15481 ttcttccggg agtactagcc aactccaacg aaacgggcgt cgcggtttat gttgccgata
    15541 atggttctac agatggctca gttaagcttc tcagatcaag gtttccgctg gtaaacctca
    15601 tcctactcga caggaattat ggctacgctg gagggtataa ccgtgcgcta agccagatag
    15661 acagcgattt ttttgtgctg cttaattctg atgttgaagt gcctggggga tggcttaatc
    15721 ctttacgtag ctttatgcgg tcaaacccaa atgcagccgc ttgcgctcct gccattatgg
    15781 acattgcccg cccggagtgc tttgagtatg ccggagcagc gggtggtttt attgacaagt
    15841 ttggctaccc gttttgtagg ggtaggattc tatccgaaat agagcaggac accgggcagt
    15901 acaaccagtc catcgaggta ttctgggcaa gcggggcctg catgatggtg agggcaaaca
    15961 ctttttggga tgcgggcggt ttcgatgacc atttttttgc ccacatggag gaaattgacc
    16021 tctgctggcg aatgttgaac atgggatact cggtatggag cattccggag tctaaagttt
    16081 accacgtagg aggaggaaca cttccaaaca acaacccata caaactttac ctgaactacc
    16141 gtaatagcct gttcatgcta cacaagaacc tatcaaagaa tgccttttac caaaccatta
    16201 tagccaggct acttctcgat tggctttcgg caatggccta cctggttcgc tttcagctta
    16261 aatttttcgg ggccgttgct aagtcacact accattacta tcgtgatatc agcaggttga
    16321 atagcttccg cagctcattc cggcatccta aaaccaacag tgcaaaagtt atctaccata
    16381 gaagcgttct ttttgatttt ttcatactcc gcaggcggaa attttccgag ctgaagtttt
    16441 agtcttttgt cagccttttt acctttttta ggataggggc gtatgttaaa aatatgaaaa
    16501 aaatcgcctt ttttgataat ctaatccttt aaaagatacc tttgcagctc aactttacac
    16561 attagccatt tagctaaact gcagcagtac caaatgatct catttaaaaa acttaccatt
    16621 cgtgatattt tattccgttc acgaatggcc tacgccgaaa gaccagctct tgcttttgtt
    16681 gactcaaagc ctgttaccta cgccaacatg gcgattattg tcaggaacat ttccgggatg
    16741 ctctacaccc ttgggattag gaagggcgat agggtagccc tcataggcga gaatagccca
    16801 aactggggta tggcttattt ggcggcaacc acaatagggg cggttgtagt tcccattctt
    16861 cccgactttt cggatacaga attggaaaac atcatacgcc atagcgaggc gagggtagtt
    16921 tttgtttcag ccaaaattta ttctcacctt aaaatcgaca atcttaaagg cgttgaggcc
    16981 ttcatcctgc tcaacagttt agttcctatt gacgaggaaa catttacagc cgatactact
    17041 attccctact ccatacccga ccctattgta aacgttgatt actgcgattg cgagttaccc
    17101 cgccctagcg aggacgacct ggcagcaatt atctatacct caggcaccac tggtaggccc
    17161 aagggggtaa tgcttaccca ccggaacctt gtttctaacg ttctatccac cttgatggtt
    17221 caggaggtaa cggagcatga caggctactc tccattctcc ctttatcgca cacctacgag
    17281 tgcaccattg gtttcctaat tccaatggca acgggtgcat cggtttacta cctcgataaa
    17341 ccacctgtag cccaaacact tattccggcc atgcaaaaag ttaggcctac aatggtgctt
    17401 acggttccgt taattattga aaaaatattt cgaggtagca tcaagcccca gctaacctca
    17461 actgccctaa ggcaacgcct ataccagttt tcgctaagtc gcaggctcct tcatcgggtt
    17521 gctgcgcgaa aactccatag tactttcggg ggtgagctta gattctttgg aattggcgga
    17581 gcaaaacttt cgcgtgaggt tgagctattc ctgcacgaag gccgatttcc ctactccata
    17641 ggctatggaa tgacggaaac atcgccgttg cttaccggta gcactcccgc acaagtaagg
    17701 tttagaacgg caggctactg cctacccggg caggagctga ggttgcttaa tcccaacccc
    17761 gaaaccggtg agggcgaaat tcttgccagg ggttccaacg taatggttgg ctactataaa
    17821 gagcccgagc taacccaagc aattttttac gagggttggt taagaaccgg agacctggga
    17881 gttatagatg acaagggata tttaaccatt aagggacggt ctaagaattt gatactgagt
    17941 tcttcgggcg aaaatatata ccccgaggaa attgaggata ttatcaacaa ccatgaatac
    18001 gttgccgatt ccgtggttta cgagtcggga ggcaagctga tggcaagggt tcacctcaac
    18061 cgggaagcag tggcgcaata cctcgccggc attaaatcct cagctgcgga cctatccaaa
    18121 aatgccgaag agcttaccag aagaattctt gacgacatca aggttatggt gaactctaaa
    18181 gtggccaggt ttgcccggct aagcgttgtt cacgagcagc ttgaaccttt cgaaaaatcg
    18241 gccacccaga aaattaagag attcatgtat caggactagt cattgtagaa ctcgtcggat
    18301 gccgtttccg tttccaggtt gaggtgtgtt ctagaattga caatgatgga gttgtaattg
    18361 catattccca tgctttttcc tggaattttt agcttgccta ctttcagata aatattttag
    18421 gtcgctgttt tggcaagttc gaccgttttt gagcattcat tccctgaaac atatttctga
    18481 ccttttttta ttataccgat taagtcggtg gcttttgtca ttttttgttt cattcgtcaa
    18541 attggccata acgg
//
LOCUS       DAOSHM010000046        18487 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_2454_length_18487_cov_21.868327, whole genome shotgun
            sequence.
ACCESSION   DAOSHM010000046 DAOSHM010000000
VERSION     DAOSHM010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 18487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18487
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2454_length_18487_cov_21.868327"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            246..977
                     /locus_tag="PK839_07775"
     CDS             246..977
                     /locus_tag="PK839_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018028861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOG72669.1"
                     /translation="MDFELTIIVPVYNEEDNLERVYQELSSYIGTAERKAKVLLVNDG
                     STDGSLAKVKEICTHDNRFFYCSFDRNYGLSAAIKAGFDYADTPWVGYIDADLQTSPQ
                     DFNLLFEYANDYDLVTGVRSMRKDSFVKNMSSLIANNIRRAFTHDGMDDTGCPLKIIR
                     TEFAKRIPMFNGLHRFLPAMILLQNGRIKQVPVRHYPRVAGKSKYHLWNRLFGPLMDC
                     FAYLWMKKKYINYHVAENNIQPPTL"
     gene            981..1604
                     /locus_tag="PK839_07780"
     CDS             981..1604
                     /locus_tag="PK839_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004350500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase N-terminal
                     domain-containing protein"
                     /protein_id="HOG72670.1"
                     /translation="MSSWIIYAIGFIAQALFSSRVFLQWIISEKHKKVLTPKLFWELS
                     LLASVLLFIYGYLRHDFPIMLGQVITYFIYIRNIQLENQWKKFPKWLRVTLFVFPFVI
                     LIYGYNNNIMDRANLFNKENIPFVLMLLGVISQVLFTLRFVYQWIYSERRKESSLPMG
                     FWVLSLAGSTLIFIYAIFRLDPVLLLGHSTGIIMYSRNIYLLKKYGT"
     gene            1594..3213
                     /locus_tag="PK839_07785"
     CDS             1594..3213
                     /locus_tag="PK839_07785"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOG72671.1"
                     /translation="MAHRYRYELLLAIVTGILLLAGLGKIEVNIMEARNFTTAREMVQ
                     NREYLLTTLNGEPRYQKPPLPTWLTALSGMAFGFDSLFTLRLPVVAITFLLVFAFYFL
                     SIALGLSKKQSFNNALILITSFYIFFGGRDNQWDMYTHAFMVTSIFFLWRLLQKDTHR
                     LGNSIAAGVFFGLSILSKGPISLYALFLPFLISYGMVYRFHLKGKGIYFINMLFIGLL
                     VGLWWYAYVRLKDPEYFRAIVGHEAANWASYETKPFYYYWNFFIQSGLWTLPSLTALA
                     YPYLKKRVDNLKAYRFSLLWTLFSVILLSAVPEKKPRYLLPTLIPMAINTGFYIDYLF
                     RRFNKGLPRAEKLWVYFSFGLLGFIALAYPFALTFILKTDILNYLPIAIISSILMLTC
                     AFFILRGMKSFNFQITFYSTIALFTVVVVAIIPLSQNLFTNPAYKPASSALNVEAKYG
                     VKTYRLNDIAPEIVWDFGKVIPSLPKINENIQAPPEKQFGLLVARSDSTIVRSGFPNF
                     EVKKVHTIDMNYRPRHRDRLIRDYYVLRLKE"
     gene            3252..3581
                     /locus_tag="PK839_07790"
     CDS             3252..3581
                     /locus_tag="PK839_07790"
                     /inference="COORDINATES: protein motif:HMM:NF021468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2185 domain-containing protein"
                     /protein_id="HOG72672.1"
                     /translation="MANYRVKPEDIKELIPQKSYCYASEMITVDGMPVGYMYREKPED
                     DDDSGWRFYSGAETEEYVEDVYHFMMFDLNYIANCDASIIPYLNSRVGTELERVEGTD
                     KFQPVED"
     gene            complement(3578..4033)
                     /locus_tag="PK839_07795"
     CDS             complement(3578..4033)
                     /locus_tag="PK839_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009126431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HOG72673.1"
                     /translation="MSLEERISQDIKTAMLAQDKVRLDALRAIKSAILLAKTEGEHKD
                     TLAPESEARLLQKQYKQRKESAEQYVQAGRQELADKELAEAAIIEEYMPKMLSADELK
                     AELVKIIGQVGAKAPSDLGKVMGVASKLLAGRADGKAISDMAKQLLGGS"
     gene            4237..5538
                     /locus_tag="PK839_07800"
     CDS             4237..5538
                     /locus_tag="PK839_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72674.1"
                     /translation="MRLRILLIFIAAFPFQNAFCQKSSYFKRVFVDAEYYLLYEEYRD
                     ALPLYQEIYKAYPSNCNLAYRIGLCYLNIPNEKKKSLPYFEKAITSITPSYQEGYFTE
                     TKAPREAFLRYGQALRIDSQFDKAMEAFKSYQNLLTPKDLLEQKIIAREIESIAYARK
                     QLANPASHTIKPVNRNVNTRFPEVNPLSDTTGNLLIYTSLQQFYNAILVSQRKEDLWT
                     NPINLNAQLFADGAIKTVGISPNGNMLILARNDNDTYNLYFSRYDRTKNTWSPIEKFP
                     KEINSSGWEISGSLSSRGDTLYFSSNRAGGFGGFDIYMSVKTITGWSNPVNLGNKVNT
                     PFDEISPFVTENGKQLFFSSNGHRSMGGFDLYLSGYENKTWGLPHNLGYPINTLDDDT
                     FLFPLNNGKRGFISRASEESSGEDDIYEVTLDLSTLLNGDK"
     gene            5673..8051
                     /locus_tag="PK839_07805"
     CDS             5673..8051
                     /locus_tag="PK839_07805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72675.1"
                     /translation="MTILKKLLPVGICLCLTIAGKPQSKLEIEEIYRDANSYFYFEDY
                     EEALTLYLQVYSHQPENYNLCYRIGFCYLNIPGSKKNAIPYLEKAAGNITRFYREESI
                     LETKAPPDAIFYLGNAYLVNNQVENAIKEYEKFWTLTRGKGSWNFDYLNHQMATAKSS
                     IQLQKNKVNFILTNLGEPVNDHFPNFNATISGNGTTLAYTSRLKFYNAIMVAQLNERG
                     EWEKPINITLDLQVDGRCETLSLSYRGDELYLFRDDNHDGNIYVSHLENGRWTPIRKL
                     NPNINSEYYETHACLSPDGKDLYFSSNRKGGFGDLDIYVSHRESGDNWEVPQNLGSTI
                     NTKLNENTPFLTSDGLSLYFSSEGHSNMGGYDIFVSQLEADGRWTAPINLGYPINTTD
                     DDLFFFPIDDGSSALMAMFDPEGMGSQDIYELKLFVPRFMRGIVSTTEVSDRISDKTF
                     NRLVVDTISFRGKALVDLTSSDIPQIIDPKLKSKLVFDGRKFDISQKISYTQTRLPKA
                     EQGENTGFQLTDARVRPNLSQPFKTFADTGMGISKLMNDTNKLISGYTKPDRIRGRSS
                     QLTPDGYAGTNVETYSYLMETLLLLTPPNLQSVLSPILKNNWQFPDNTLSESILNFSS
                     AFKTYEGRDAMVRALANLSDVISDKTSGNTSQRRQTIASDSEYGSFTYFYNQLINLSS
                     PNLAKQLAAITVGHPKINTFSALLAAYKKANPKGYRENLPELLRVFSRISISAYNALP
                     DEDKINLYSDITQDVTSGKRNVWYFYLLLFLVVALAGYLLFRYLKLRKESQS"
     gene            complement(8477..8668)
                     /locus_tag="PK839_07810"
     CDS             complement(8477..8668)
                     /locus_tag="PK839_07810"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HOG72676.1"
                     /translation="MFAGGKSSRMGKRLLPVNGKPLIQHIAEQLVDFLDEAIIGANDT
                     EKYGFLKLRVDPDIWRKAL"
     gene            9102..9350
                     /locus_tag="PK839_07815"
     CDS             9102..9350
                     /locus_tag="PK839_07815"
                     /inference="COORDINATES: protein motif:HMM:NF025468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4276 family protein"
                     /protein_id="HOG72677.1"
                     /translation="MSAPKKYGLQKLLAEKVNPELINDNPETAPSKRIIKLIPEYDKV
                     SVGAVIVGEIGIDFLKKTCSHFNGWIAKLENLSSITNR"
     gene            complement(9515..10438)
                     /locus_tag="PK839_07820"
     CDS             complement(9515..10438)
                     /locus_tag="PK839_07820"
                     /inference="COORDINATES: protein motif:HMM:NF025729.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4412 domain-containing protein"
                     /protein_id="HOG72678.1"
                     /translation="MNKLTTTIIMVSFMITAFSGESKSQVGGMLKDRLKKAAVKSVLS
                     NQDEGQSDTISEKQQNQQAENPTETYMQAKMMGLMGKGNVKYDLSYTFTSSMKMDFEV
                     LDSAQNEPFTGTYTLYFEKDGKNFAMEVETAGKKDEPASKSLFIYDFRNLALLILSEQ
                     EGQNSGMAMAIPPDSSKVEPSQVAPTTDTELPGNEVSTGDVTFRATGRTKSILGYSCK
                     EYLMENPEGKVELWVTNDVRYDYSDAYNRMQGFQMMSNVNSSATNGTVLEMHFKDSGS
                     PAHSDFYVREINKDKSQTFNLAGYTIMSFGQ"
     gene            10623..11846
                     /gene="hydF"
                     /locus_tag="PK839_07825"
     CDS             10623..11846
                     /gene="hydF"
                     /locus_tag="PK839_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HOG72679.1"
                     /translation="MQRGKESKPHIGIYGRRNNGKSSLINLLAGQNVAIVSDFAGTTT
                     DPVKKSFEITGFGPVILVDTAGIDDSGELGEKRVGKTLETINQVDLAILVVAGNTWGD
                     YEERLIEEFSTSDTPFLVIHNKSDLTPASEEFKRVVGSKPHRGFAEISCLWPNNVDTL
                     VEMIKGAIPESSYQVPTLLGDLISYGDVVLLITPIDVEAPAGRLILPQVQAIRDVLDN
                     DAVAIVLKEREVDDFLRKTRIKPALAVTDSQIFSKADASIPRDIPLTSFSILLARMKG
                     DFASYLKGTPKISQLKNGDRVLLLESCSHHVSCDDIGRTKIPRWLSNFTGKQLEYDVV
                     AGLSSLPRSITDYALVIQCGGCMITRKQLINRIRPAVEAGIPVTNYGMAIAYVQGIYQ
                     RAIAPFAPASPTSYL"
     gene            11932..12639
                     /locus_tag="PK839_07830"
     CDS             11932..12639
                     /locus_tag="PK839_07830"
                     /inference="COORDINATES: protein motif:HMM:NF017970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HOG72680.1"
                     /translation="MEKFKSDLSGQEFPISERVSGKSIRQPIMELIQKDFPQFSAEEF
                     LSLTELNDYREKYITEYLVRQTGELTELEQKVIDSISDRATLSDKLVEDETQRTFGQK
                     IADKVAAFGGSWTFIIAFLGFILIWICLNVFWLANRNFDPFPFILLNLILSCIAAIQA
                     PVIMMSQNRQESKDRERAKKDYMINLKAELEIRSLHEKIDHLILRQQQEIVEMQKVQT
                     DMLSDIAKEVVSSSERE"
     gene            complement(12654..15137)
                     /gene="priA"
                     /locus_tag="PK839_07835"
     CDS             complement(12654..15137)
                     /gene="priA"
                     /locus_tag="PK839_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016277374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HOG72681.1"
                     /translation="MAQAGDCFVDVVLPLALPRLYTYSIPDEMIGRVEQGMRVVVSFG
                     RKKLYAAVVFRVHSDAPNGYQTKDIIQLIDEQPVVLPGQLKFWSWVADYYMCYLGEVM
                     KAALPSGLKMESESFVSQANVEDINFSDSERIILHLLDGKKKRRIQDIATMAGFKNPM
                     GAIKSLLEKNAIFISEEIEEAYRPKTEKYLSISPKYCSNDAINELLNSLGRAPAQQRL
                     LLGMLSLIDNFSELSDVCVPKRKVLEKVDVSAAVVGSLVAKGVICEVEKAVSRVNDAD
                     NQLGKPVELSSAQLEALNRVKESFATHHVTLLHGVTSSGKTEVYIHLIGEALAEGKQV
                     LYLLPEIALTTQIISRLRRIFGDRVGVYHSKFSDSQRVEVYNGVLRAESTPGKPAYSV
                     ILGVRSSVFLPFRNLGLVIVDEEHENTYKQFNPAPRYQARDAAIMLAGFHGAKVLLGT
                     ATPSVETYFNARTGKYGLVELADRYLNMELPQVEVVDVREARKRKLMRSHFSEKLIEE
                     IGLSLERNEQVILFQNRRGFAPFIECHECGWVPHCENCDVTLTYHKASDLLVCHYCGY
                     SVKPTHECMACGSTNLQPQGFGTEKVEDELQIFFPEASIERIDLDSTRSRTAYEGIIA
                     RFETGQTDILIGTQMVTKGLDFDRVSLVGVLNADNMLNFPDFRASERSFQLMAQVSGR
                     AGRKNRRGLVIIQTSQPNHPVIKQVKENDYMAFYATVVGERQKFLYPPFCRLISISIK
                     HKDQNVARQASEVMANGLKHSLGNRVLGPEAPMVGKIQGYYIQSILVKAGGQQSLSRV
                     KELIAYMANSLRVNGEYRSLVVAVDVDPQ"
     gene            15466..15539
                     /locus_tag="PK839_07840"
     tRNA            15466..15539
                     /locus_tag="PK839_07840"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15500..15502,aa:Pro,seq:ggg)
     gene            complement(15613..16449)
                     /locus_tag="PK839_07845"
     CDS             complement(15613..16449)
                     /locus_tag="PK839_07845"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HOG72682.1"
                     /translation="MKDRQLDIAVISDLHLATYACKPKKILKYLKSIKPQMLVLNGDI
                     IDSWRFSRNYFPKSHLKVVRQIIKMMEKGVKVYYITGNHDEFLRKFSPVEAGNLKIVD
                     RLVLDQDGGKTLILHGDIFDHSIYMTKWLAKIGAAGKGMLSLIDAFINGLLGIFGRKD
                     FILYKTINQKLNRERSTLIGYEKSMLKSCAEQGYQTVICGHTHFPKDKELLINNKRVH
                     YLNCGDWVEHFTAAEYYNGSWHLYVQNETGDELLYDEPEIPAGRQLYQKVKLELAFSN
                     LG"
     gene            16689..18299
                     /locus_tag="PK839_07850"
     CDS             16689..18299
                     /locus_tag="PK839_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009017712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HOG72683.1"
                     /translation="MKQPKKKREFPHTYVIIFTLIVVAALLTWFIPGGEFTREVKSIN
                     GIDREVIVPGSFHQVDNSPQTWQVFTSIFQGMRRTCDIIFYILIIGGSFWMLNESKAL
                     DVAILSFLNFTRRIERFKPLKAIGVNNLVIALIMICFSFFGAVIGMSEETIAFVVIFV
                     PLAISMGYDSIVGICLCFLGAGLGFASALLNPFTIGIAQGLSGIPLFSGIEYRFIIWL
                     VINTIGISYVLWYANRIRKNPKLSPVYEIDEHWRHKASEEGAIVKTKAGKIAWVVFAV
                     VAIVLAIFAYLYPTSQLVVGQNVISVPAIPIATVVWVILGLLALRHSVQLFIVNILMF
                     TILFLIVGVMGYGWYIKEIASLFLVMGILASAAFGKNANQLTRSFIDGARDIMSAALV
                     VGLASGIVVVLEQGKVIDSLLNYSANAMQGYGKVTSMAIMYIFYNLLNLIIASGSAKA
                     ALTIPLMAQFSDLIGISRQATVTVYQLGGGFTNLITPTSGVMVGVLSIARIPYNKWFR
                     WFLPLMFILIVAGFLLLLPTLFLPLSGF"
BASE COUNT         5002 a         4596 c         3975 g         4914 t
ORIGIN      
        1 tccaacgggt tagtgtggtg taaaaacttc cataaagcca tttaactttt gactttcccc
       61 cgctcgcttt caagcccaat aatctttttc tcagggaaaa ctcttgtgca tgaattttgt
      121 tcccctaata agaacaacat tttagcagct tactaaaagt gtaaaacctt ttatactttt
      181 acaactttga aatagctggc tatttttagt gaatcctaag gcaacaacgg aattatctaa
      241 acaatatgga ttttgagctg acaatcattg ttccggttta taacgaggag gacaacctgg
      301 aacgagttta ccaggagcta agcagctaca tcggaacagc tgaaaggaaa gctaaggtgc
      361 tgttggtaaa cgatggctcc accgacggca gccttgccaa ggttaaggaa atctgcacgc
      421 acgacaaccg cttcttctac tgttcatttg accgtaatta cgggttaagc gcagccatta
      481 aggccggctt cgactatgcc gacacgccat gggtcggcta cattgatgcc gacctgcaaa
      541 cctcacctca ggatttcaac ctcctgtttg agtatgccaa cgactacgac ctggttacag
      601 gcgtcaggtc tatgcggaag gactcctttg tgaagaacat gtcgtcgctc atagccaaca
      661 atatacgcag agcctttacg cacgacggaa tggacgatac cggctgcccc cttaaaatta
      721 tcagaacaga attcgccaag cgtattccca tgtttaacgg ccttcaccgt ttcctcccgg
      781 ccatgatact cctgcaaaac ggacgcatta agcaggtacc tgtgaggcac tatccacgcg
      841 tagccggcaa atcgaagtac cacctctgga acaggctctt tggacccctg atggactgct
      901 ttgcctacct atggatgaaa aagaagtaca tcaactacca tgtggccgaa aacaacattc
      961 aacctcccac cctataaccc atgagcagct ggataatata cgccattggg tttatagcac
     1021 aagcgctttt ctcaagtcgg gttttcctgc aatggattat ttccgaaaag cataaaaagg
     1081 tactcacccc aaagctattc tgggagttga gcttgctggc gtcggtgctg cttttcattt
     1141 acgggtactt gcgccacgac ttccccatca tgctgggaca ggttatcacc tacttcatct
     1201 acatcaggaa cattcagctg gaaaatcagt ggaaaaaatt tcccaaatgg ctacgtgtta
     1261 ccctcttcgt ttttccgttc gtcatcctga tatacggcta caacaacaac atcatggaca
     1321 gagccaacct gttcaataag gaaaacatac ccttcgttct catgctgctt ggggtgatat
     1381 cgcaggtgtt gttcaccctg aggtttgttt accaatggat atactccgag cggagaaaag
     1441 aatcgtcgct gcccatggga ttttgggtgc taagcctagc cggttctacc ctaatattca
     1501 tttacgcaat ttttaggctt gaccctgtgc tgctgctcgg acattcaacc ggcattatca
     1561 tgtactcccg aaatatctac ctgttaaaaa agtatggcac ataggtatcg ttacgagttg
     1621 ctgctggcca ttgtaaccgg gattctgctt ctggcaggcc ttggtaagat tgaggtcaac
     1681 attatggagg caaggaactt cactactgcc cgcgagatgg ttcagaacag ggagtacctg
     1741 ttgaccaccc tcaacggtga gccccgctac cagaagccgc ctttgcccac ctggttgacc
     1801 gccctttcgg gaatggcatt cggtttcgac tcacttttta cccttcgact tccggttgtt
     1861 gccataacat tcctactggt ttttgccttc tacttccttt ccatagcgtt gggtttgtca
     1921 aaaaaacaaa gcttcaacaa cgcccttatc ctgataacct ctttctacat attctttggt
     1981 ggaagggata accagtggga tatgtacacc catgccttta tggtaacctc catattcttt
     2041 ctgtggaggc tactacaaaa ggatacccat cgcctgggca atagcattgc cgccggagta
     2101 ttttttggat tgtcaatact aagcaaaggc cccatctcac tctatgccct tttcctgccc
     2161 tttctaatca gctatggcat ggtttaccgt ttccatctta aggggaaagg catctacttt
     2221 ataaacatgc ttttcatcgg attgctggtt ggcttatggt ggtacgcata cgttcggttg
     2281 aaggatccgg agtacttccg ggctatagtt ggacatgagg ctgcaaactg ggccagctac
     2341 gaaacaaaac ccttctacta ttattggaat ttcttcatac agagcgggtt gtggacacta
     2401 ccctcattaa ccgcactagc ctatccatac ctcaaaaaaa gggtggacaa cctcaaggcc
     2461 tacagattct cgctgctttg gacgctgttt tcagttattc tactctcagc cgttcccgag
     2521 aagaaacccc gttacctgct acccacccta attccaatgg ctatcaacac aggcttttac
     2581 atagattacc tttttcgaag attcaacaaa ggtttgccaa gagccgagaa gctgtgggtt
     2641 tacttttcat ttggactatt aggattcatc gctctggcat accctttcgc cttaacattc
     2701 atccttaaaa ctgatatact aaactaccta cctattgcca tcatctcgtc aatactcatg
     2761 ctaacatgcg catttttcat tctcaggggg atgaaaagtt ttaacttcca aatcacattc
     2821 tactccacta ttgccctctt tacagtggtt gtggtggcta taattccatt atcgcaaaat
     2881 ttgtttacca atccagccta taagcctgca agcagtgcat tgaatgtaga agccaagtat
     2941 ggcgtaaaaa cctaccggct aaacgacatt gcccccgaaa ttgtatggga ttttggcaag
     3001 gtcattcctt ccctacccaa aattaacgaa aacatacaag ctccgccaga aaagcagttt
     3061 gggctgcttg ttgcccgaag cgattcaacc atagttaggt caggcttccc gaattttgaa
     3121 gtaaaaaaag ttcacaccat tgacatgaac tatagacccc gacataggga caggcttatt
     3181 agagactact acgtattgag gttgaaagaa taacggtaat caaataaatt gcaaaagtta
     3241 aacctaaagc tatggcaaac tacagggtta aacccgagga cataaaggaa ctaattcctc
     3301 aaaagtctta ctgctacgcc tcggaaatga ttacagttga tggaatgcct gttggataca
     3361 tgtataggga gaaacccgag gatgatgacg atagcggctg gcgtttctac tcgggtgccg
     3421 aaaccgagga gtacgttgaa gacgtttacc acttcatgat gtttgacctc aactacattg
     3481 ccaactgcga tgcctccatt attccctacc ttaactcaag ggttggcact gagctggagc
     3541 gggttgaggg aaccgataag tttcaaccgg tagaggatta gctaccaccc agaagctgtt
     3601 tagccatgtc ggaaatagcc tttccatcag ccctgccggc tagcagcttg ctggcaacac
     3661 ccataacttt tcccaggtcc gaaggtgcct tagcgccaac ctgcccaatt atttttacca
     3721 gctcagcctt cagctcatca gcgctaagca ttttgggcat gtactcctct atgatggcag
     3781 cttcggctag ctccttgtcg gccagctcct gccggccagc ctgaacgtac tgctcagccg
     3841 actccttcct ctgcttgtat tgcttttgca gcaacctagc ctcggattca ggagcaagcg
     3901 tatccttatg ctcgccttct gtttttgcaa gcaaaatggc actttttatt gcccgtagcg
     3961 catccagcct aaccttgtcc tgtgccagca tggctgtttt gatatcctgg ctgattcttt
     4021 cctctaacga catctcctcg caaattatgg tttctgacac aaagttagaa aaagttggaa
     4081 taatttgact ttaactattt atttgaatta aaataattta ctacttttga aagttgttta
     4141 gctatttgtc atatcttgct ggttgtttta aaattgaggt gttatttcta aacaatttgg
     4201 ttggataaat cctttagggt tgtaaactgc cgattgatga gactgaggat tttgttgata
     4261 tttatagctg cctttccatt ccaaaatgcg ttttgtcaga aaagctccta ctttaaaaga
     4321 gtttttgttg atgcggagta ctacctgctt tacgaggagt atcgtgacgc gctacctctt
     4381 taccaggaaa tatacaaggc ctacccttca aactgtaacc tggcctaccg catagggctt
     4441 tgctacctca acattcccaa cgaaaaaaag aaatccctac cctatttcga gaaggctatt
     4501 acctccatca ccccatcata ccaggaaggc tatttcaccg aaaccaaagc accgcgtgaa
     4561 gctttcctac gttacggaca agccttacga attgactcgc agttcgataa ggccatggag
     4621 gcttttaaat cgtaccaaaa ccttcttact ccaaaggatc tcttggagca aaaaataatt
     4681 gccagggaaa ttgagtccat cgcctacgcc aggaagcagc tcgccaaccc cgctagccat
     4741 accattaaac ccgttaaccg gaatgtgaat acccgtttcc ccgaggttaa ccccctttcc
     4801 gatacaacgg gcaacctgct catctacacc tctcttcagc agttctacaa cgccattctg
     4861 gttagccagc gcaaggaaga cctctggacc aaccccataa atcttaacgc ccagctattt
     4921 gccgatgggg ctattaaaac tgtgggcatt tcacccaacg gaaacatgct tattctggcg
     4981 cgtaacgata acgacaccta taacctctac ttcagccggt acgacagaac aaaaaacaca
     5041 tggtcgccca ttgagaagtt cccaaaggag attaactcca gcggatggga aatttcgggc
     5101 tcgctctcca gtcgtggcga taccctatac ttcagcagta acagggcagg tggttttggc
     5161 ggattcgaca tctacatgtc ggtgaaaacc ataacaggtt ggtctaaccc ggttaacctg
     5221 ggaaataaag tcaatactcc tttcgatgaa atatccccat ttgttaccga aaatggcaag
     5281 cagctcttct tcagctccaa cggacaccgc tccatgggag gattcgacct gtacctctcc
     5341 ggttatgaga ataaaacatg ggggttaccc cataacctgg gctaccctat caacaccctt
     5401 gacgacgata catttctatt cccactcaac aacggtaaaa gaggatttat ttctcgagcc
     5461 tcggaggagt caagcggcga ggacgacatc tacgaggtaa cccttgacct cagcacctta
     5521 cttaacgggg ataaatgacg gattgttaca atagcggcat aaagcaacta taacgtgtcc
     5581 aactcatatg aaaaatgttt tgagcttaga gcctcattcc gcagggacaa acccgtaaag
     5641 aaatgaacct ttgaaggaaa acaagtgcat caatgacaat cttaaagaaa ctcctacctg
     5701 ttggaatttg tctatgcctc accatagccg gaaaaccaca atccaaatta gaaattgagg
     5761 aaatatatcg tgacgccaac tcctactttt acttcgagga ctacgaagag gccttgacat
     5821 tataccttca ggtatatagc caccaacccg aaaactacaa cctttgctac cggattggct
     5881 tctgctacct gaacattccg gggagtaaaa agaatgccat tccctatctg gaaaaggcag
     5941 ctgggaatat tacccgtttt tacagggagg agtctattct tgagacaaag gccccacccg
     6001 atgcaatttt ttaccttgga aatgcctacc tggtaaataa ccaggtggaa aatgctatta
     6061 aggagtatga aaaattctgg acgctgacaa ggggtaaggg tagctggaat tttgactatc
     6121 taaatcatca gatggccaca gcaaagagct ccatacagct gcagaaaaat aaggttaact
     6181 tcattctgac caatttggga gaaccggtaa atgaccactt tcccaacttc aacgccacca
     6241 tttcgggcaa tggaaccacc cttgcataca catcaaggct aaaattctac aacgccatca
     6301 tggtggcgca gctgaacgag cggggtgagt gggaaaaacc catcaacatc accctcgacc
     6361 tgcaggttga cggccgatgc gaaacccttt cgctatcgta caggggcgat gagctctacc
     6421 tcttcaggga cgacaatcat gatggaaaca tttatgtttc ccatctggaa aatggcagat
     6481 ggacaccaat tagaaagctc aacccaaaca tcaactccga atattacgaa acacatgcct
     6541 gtcttagccc cgatggcaag gacctatact tcagcagcaa ccgaaagggt ggattcggcg
     6601 atctggatat ttacgtcagc caccgggaat ctggagataa ttgggaagtc ccccaaaacc
     6661 ttgggtcaac aatcaatacc aaacttaacg aaaacactcc attcctcacc tccgatggat
     6721 tatccctgta cttcagctcc gagggccata gtaacatggg gggctacgat atctttgttt
     6781 cgcagctgga agctgatggc cgctggaccg cccctattaa tctgggatac cctatcaata
     6841 ctactgatga tgatcttttc ttcttcccga ttgatgatgg ctccagcgca ttaatggcca
     6901 tgtttgaccc cgaggggatg ggctctcagg atatttacga gctgaaactg tttgttccca
     6961 gattcatgcg aggcatagtg tcaactaccg aggtatccga cagaatctcc gataaaacgt
     7021 ttaaccggtt ggtggttgac accattagct ttagaggtaa agctcttgtt gacctcacca
     7081 gctccgatat accccaaatt attgacccca aacttaaaag caagcttgtc ttcgacggta
     7141 ggaaattcga cattagccag aaaattagct acacccagac acgcttaccc aaggcggaac
     7201 aaggagaaaa taccggtttt cagctaactg atgctagggt aagaccaaac cttagccaac
     7261 cctttaagac ctttgccgat acgggcatgg gcatttctaa gctgatgaat gacacaaata
     7321 aactgatttc tggctacacg aagcctgacc gtataagggg taggagtagc cagcttaccc
     7381 ctgatgggta cgccggcact aacgtcgaaa cctacagcta cttaatggaa acgctgctac
     7441 tactaactcc tccaaatttg cagtctgttc taagccctat actcaaaaac aactggcagt
     7501 tcccggataa cacccttagc gagagcattt taaatttttc cagcgcattt aaaacctacg
     7561 aggggagaga tgccatggtg agagcactgg ccaacctcag cgatgttatt tctgacaaaa
     7621 cctccggcaa cacctcccaa agaagacaaa caattgcttc cgactccgag tacggctcat
     7681 tcacctactt ttacaatcag ctaatcaacc tttcctcccc aaatctggcc aaacagctag
     7741 ctgccattac tgttggccat ccaaaaatca acaccttttc tgccctgctt gccgcctata
     7801 aaaaagccaa cccaaaaggc tatagggaga acctccccga gctgcttaga gttttttcac
     7861 gaatttcaat cagcgcttac aatgcactgc ctgatgagga taaaatcaac ctgtacagcg
     7921 atatcacaca ggatgtaacc tcaggaaaga gaaatgtttg gtacttctac ctgttgctat
     7981 ttctggttgt ggcattggct ggttacttgc tatttaggta cctgaagcta aggaaagaat
     8041 cgcaatcgta ggaggcattg gagttcttcc ccctggttgg aactttttga tactcctgta
     8101 tagctggtgt tgcccgcaac cacctccaga aaaaattatc tacctacgta ctcaaaataa
     8161 tcgctagcgc agtttaggtt ttgataccat ccattgccag taaaatcaac agtagcgtat
     8221 tcagtacaat caagaagctc aacagtcctt tgcctgtttt catccaaaag caatttggcc
     8281 ttagcagcaa cgcttttacg ttatacgaca aaaaggggtt cgaaccgtga ggaatcattc
     8341 acaggtatta ctatctcgta atctaacgcc aagttggcca ttctacttac tagctcaata
     8401 ttcatttccg ggatatcgca ggctgtagta aagtttatgt cgctgtccga agccagcaaa
     8461 catgagtaaa ttcacattaa agtgcctttc tccaaatatc aggatcaacc ctcagcttta
     8521 ggaagccata cttttccgta tcgtttgccc cgataattgc ctcgtcgaga aaatcaacca
     8581 gctgctctgc gatgtgctga attagaggtt ttccgtttac agggagcaac ctcttaccca
     8641 ttcgggaact tttgcctccg gcaaaaatta ttgcggttgc tttttgacga atatgctatg
     8701 ttccgtcttt aacaaatata cgccctggct caaaaaccca ggagttattt tgaaagccaa
     8761 taattttatc ggcaggctaa ttacttttga gcagcccttt tttacccctt gtttctccta
     8821 aatttcttat tacaatgaac agacccgtgt caaggacttt tcgaatcgaa tttaattcca
     8881 ccactaccga aacattctga ggaatagaat cgtgcgccaa ccaaaagatt ttccaaaata
     8941 taatgagtca aaaaaaatta atgatgccta tgaacgggtc gaattcctcg aaaaagcatt
     9001 tgcagatgat attcaagata atagatttat accctacctt cagctatagg aatttgaaac
     9061 actcatacta tcaaaacctg aagaattaaa aattgaatat tttgagcgct ccaaagaaat
     9121 atggcttaca gaaacttctc gctgaaaagg tgaatccaga gctaattaat gataaccccg
     9181 aaacagcacc ttcgaaaaga ataataaaac ttattccgga atacgataaa gtaagcgttg
     9241 gggcagtaat tgttggggaa atagggattg actttctaaa aaaaacatgc agccatttta
     9301 atggttggat agctaagctg gaaaaccttt ctagcattac caaccggtaa taacaactaa
     9361 acggtagaaa tgacaacctt gaccattgcg tcaggttaga tattcatcta gctccatatt
     9421 cctacagcgc tccccctgca ataaactgcc gtaaatggca aatgccgcaa tcatttgaag
     9481 taatgcggta agtgcctcca aatcctcaac cagcctactg cccaaagctc ataatggtgt
     9541 agccggccag gttaaatgtt tggctcttgt ccttgtttat ctccctgacg taaaagtcgg
     9601 aatgcgcagg tgaaccagaa tccttgaagt gcatttccag cactgttcca ttggttgctg
     9661 atgagttcac gttcgacatc atttggaatc cctgcatccg gttataggca tcggagtagt
     9721 catagcgcac atcgtttgtt acccaaagtt caaccttacc ctcggggttc tccataaggt
     9781 actccttaca gctgtagcca aggatgcttt tggttcgtcc ggttgcccta aaggttacat
     9841 caccagttga aacttcgttg ccaggtagct cagtgtccgt ggtaggcgcc acctgagaag
     9901 gttctacctt agaagaatcg ggtggaatag ccatggccat gccgctattt tgcccttcct
     9961 gctcgctaag aatcagcagg gccaaattgc ggaaatcata gatgaatagc gattttgagg
    10021 ctggctcatc ctttttgccg gcagtctcaa cctccatggc aaaatttttg ccatcctttt
    10081 caaagtaaag ggtgtaggtt cctgtaaaag gctcattctg cgctgaatct agcacctcaa
    10141 aatccatctt catgctcgag gtgaaggtgt aggataaatc gtacttcacg tttccctttc
    10201 ccatcaaccc catcatcttt gcctgcatgt aggtttcggt tgggttttcg gcctgctggt
    10261 tttgctgttt ttcactaatg gtatcgcttt gaccctcatc ctgattggat agcacactct
    10321 ttacagcagc ctttttaagc ctatccttaa gcattccccc cacctgcgac ttcgattccc
    10381 cgctaaaagc ggttatcata aatgaaacca ttataatggt tgtagttaac ttattcatgg
    10441 tatgttattt ataaggaatt acatgcaatt tagccgatag ttttgttaaa tgcaaattat
    10501 tttcattctt taatgttgat attaagattc ttgataccga catgaagctt gtgcataatg
    10561 tttaacaagg aacgaataaa tagccgacga atgcctaaat ttgccaacaa acattccagc
    10621 ccatgcagag aggaaaagag tcaaagccac acattggcat ttatggaaga cgcaacaacg
    10681 gaaaaagctc gcttatcaac ctgcttgcag gtcagaatgt agcaatagta tccgattttg
    10741 ccggaaccac aaccgaccct gtgaagaaat cgtttgagat tacgggcttt ggaccggtta
    10801 ttctggttga taccgccgga attgacgata gcggcgagct gggggaaaaa cgggttggta
    10861 aaacattgga aacaatcaac caggtagatt tggctattct ggttgtggca ggtaacacct
    10921 ggggtgatta tgaggaaagg ctgatcgaag agttttcaac cagcgatacc ccctttttgg
    10981 tcatccacaa taaatccgat ttaacccctg ccagtgagga gttcaaacgg gttgtgggtt
    11041 ccaaacccca caggggcttt gcggaaataa gctgcctttg gcctaacaac gtggatacgt
    11101 tggttgagat gataaagggg gctattcccg aatcgtcgta tcaggttcca acccttttgg
    11161 gtgatttaat atcctacggc gacgtggtgc tccttatcac tccgattgac gttgaggctc
    11221 cagccggaag gcttatacta ccacaggttc aggccatcag ggatgtgctt gataacgatg
    11281 cggtggccat agtgctgaag gagcgtgagg ttgacgactt cctcagaaaa acccgtatca
    11341 aacctgcgct ggctgttacc gatagccaga ttttctccaa ggccgatgcc tccatcccga
    11401 gggatattcc ccttaccagc ttcagcatac tgcttgcaag gatgaagggt gattttgcca
    11461 gctacctgaa gggaacccca aaaatttcgc agctaaagaa cggcgacagg gtgttgcttc
    11521 tcgaaagctg tagccaccac gtttcctgcg acgatattgg ccgtacaaaa atccccaggt
    11581 ggctttccaa cttcaccggt aaacagctgg agtacgacgt ggtggcaggg cttagctccc
    11641 taccccgcag catcaccgat tacgcactgg ttattcagtg tggcggctgc atgattaccc
    11701 gcaagcagct tatcaacagg ataagacctg ccgttgaagc gggaatacca gttaccaact
    11761 acggtatggc cattgcctat gttcagggaa tataccagcg tgccattgcg ccctttgctc
    11821 cggctagtcc aaccagctac ctatagttga gtttacccgt tttaccccag cttaatatag
    11881 cagtccgaaa cttcaaatct ccttcaggta cgaacaaatt aatgaaaggt tatggaaaaa
    11941 ttcaaaagtg acctatcggg tcaggaattt ccaatttctg aacgggtttc aggtaaatca
    12001 atccgccagc cgatcatgga gctgattcaa aaggatttcc ctcagttttc agccgaagag
    12061 ttcctatcgc taacagagct taacgattac cgcgagaagt acatcaccga atacctggtg
    12121 aggcaaaccg gcgagctcac cgagcttgag caaaaagtga tagactccat atccgaccgg
    12181 gcaacccttt cggataagct ggttgaggat gaaactcaac gtactttcgg ccagaaaatt
    12241 gccgacaagg ttgccgcatt cggtggtagc tggaccttca ttattgcttt tctgggtttt
    12301 attttaatct ggatttgcct gaatgttttc tggctggcca accggaactt cgaccctttt
    12361 ccattcatcc tgctcaacct cattctatcg tgcattgctg ccattcaggc acctgttatt
    12421 atgatgagcc aaaaccgcca ggagtctaag gatcgggaaa gggctaaaaa ggactacatg
    12481 ataaacctta aggctgagct ggaaatcagg tcgcttcacg aaaaaattga tcacctgatt
    12541 ttacgccagc agcaagaaat agttgagatg cagaaggttc aaactgatat gctcagcgac
    12601 attgccaaag aggtggtaag tagctcggaa agggagtaaa gagatagccc tccctactgg
    12661 ggatcaacat caaccgcaac aaccagcgaa cggtactcgc catttaccct caggctgttg
    12721 gccatgtagg caataagctc cttaaccctc gaaagggatt gctgcccgcc agccttaacc
    12781 agaatgcttt gaatgtaata accctgaatt tttccaacca tcggggcttc tggcccaagc
    12841 accctgttac ccaagctatg cttcaaccca tttgccatta cttcggaagc ctgtcgggca
    12901 acattttggt ccttatgttt aatcgaaatg gaaattaacc ggcaaaatgg tgggtagagg
    12961 aacttctgtc gctccccaac tacagtggcg taaaaagcca tgtagtcgtt ttccttcacc
    13021 tgcttaatca ccggatggtt aggttgcgat gtttgaatga ttaccaagcc ccgtcggttc
    13081 ttacgcccgg cacgtccgct tacctgcgcc atcagctgaa aactcctttc ggatgccctg
    13141 aaatcgggaa aattaagcat gttgtcggca tttagtacac ccaccaggct aaccctgtcg
    13201 aaatccaagc cctttgtaac catttgtgtt ccgatgagta tatcggtttg ccccgtttca
    13261 aaccgggcaa taattccctc gtaggcagtt cgtgaacggg ttgagtcaag atctatgcgt
    13321 tcaattgaag cttcggggaa aaatatttgc agctcatcct ccaccttctc ggttccaaat
    13381 ccctgcggtt gcaggttggt gctaccacat gccatgcact catgggtagg tttaacagag
    13441 tagccgcagt agtggcacac cagcaggtcc gatgccttat ggtaggttag ggttacgtcg
    13501 cagttctcgc agtggggaac ccagccgcac tcatgacact cgataaatgg cgcaaagcct
    13561 cgcctgttct gaaacagaat gacctgctca ttgcgttcaa gggatagccc gatttcctca
    13621 ataagctttt cggagaagtg cgagcgcatc agcttgcgct ttcgggcctc ccgtacatca
    13681 accacctcca cttggggcag ctccatgttt aggtagcggt cagcaagctc caccaaacca
    13741 tacttacccg ttcgggcgtt aaagtaggtt tcaaccgatg gtgtggctgt cccgagcagt
    13801 acctttgcgc catgaaaccc ggccagcata atggcggcat cacgggcctg gtatcgcgga
    13861 gcggggttaa actgcttata ggtgttttca tgctcctcgt caacaatcac caacccaaga
    13921 ttcctgaagg gtaagaaaac cgatgagcga acaccaagaa tcacgctgta ggcaggtttt
    13981 cccggagtac tttcggcacg cagcactccg ttgtacacct caacccgctg cgaatcgctg
    14041 aacttggagt ggtacacccc taccctatcg ccaaatattc ttctcaacct gctgattatc
    14101 tgtgtggtaa gtgcaatctc gggtagcagg tacagcacct gtttaccttc tgcaagcgct
    14161 tcgccaatta aatgaatgta cacctcagtt tttccgctag aggttacacc atgaagcagg
    14221 gttacatggt gggtggcaaa cgattcctta actctattca aagcttcaag ctgagcactg
    14281 ctcaactcca ccggctttcc cagctggttg tcggcatcgt taacccgcga aacagccttt
    14341 tcaacctcac aaattactcc tttggcaaca agactaccaa caacagctgc tgagacatca
    14401 actttttcga ggactttgcg tttcggcaca caaacatcgg acagttcaga aaaattatcg
    14461 atcaacgaaa gcatcccaag cagcaacctc tgctgagccg gagccctgcc aaggctatta
    14521 agcaactcgt tgatagcatc gttgctacaa tattttggtg aaattgacaa gtacttctcc
    14581 gtttttggtc tgtacgcctc ctcaatctcc tcggagatga agattgcatt tttctccaac
    14641 agcgacttaa tcgcccccat aggattttta aaacctgcca tggtggcaat atcctgtatt
    14701 ctacgcttct tcttaccgtc aagcaggtgc aggataatcc gttcgctatc cgaaaagtta
    14761 atatcttcaa cattcgcttg gctcacaaag ctttcgctct ccatcttcag gccggaggga
    14821 agtgcagcct tcatcacctc acctaaatag cacatgtagt agtcggccac ccaactccaa
    14881 aacttcagct ggccgggtag tacaaccggc tgctcgtcaa taagctggat gatgtccttg
    14941 gtttgatacc cattgggggc atcactatga accctgaaca caaccgcagc gtaaagtttc
    15001 tttcgtccaa acgaaaccac aacccgcatt ccctgttcaa ccctacctat catttcatcg
    15061 ggaatggagt aggtgtagag ccttggaagc gctagtggaa gcacaacatc aacaaagcaa
    15121 tctcctgcct gagccattac ctgccactcc aattagccat tacctgatgt aaaaaaaaac
    15181 aatgagccct gcatcattaa aaactcgtgt gtaaaggaaa atatgcaagt agaaaccatc
    15241 tgcaaggtgc agccggattt gttacacaag cccttcgcct agccgggaga gctggcttcc
    15301 taaagtaaaa catacgggca aggtatgaaa atctttgcag gattgaataa aaatttttga
    15361 aacaaactaa aaagggtgta tttttgcaac gctttgctca gaaattcgag agatggctaa
    15421 ttttttcaac cattttcggt tatgaagcag ggcgaaattt tgcttcgggg tgtggcgcag
    15481 ttggctagcg cacttgcatg gggtgcaagt ggtcgtccgt tcgagtcgga tcaccccgac
    15541 aaaacagtaa aaggcaaccg gtggttgcct ttttaatttc aatacccggc tatttaaaat
    15601 ttacccggcg agctacccca ggttagaaaa tgccaactcg agtttaacct tctggtacag
    15661 ctggcgacct gctggtattt ccggctcatc atatagcagc tcatccccgg tttcgttttg
    15721 aacgtaaaga tgccaggaac cattgtagta ctcggctgca gtgaagtgct ccacccaatc
    15781 gccacagttc aggtagtgaa ccctcttgtt gtttataagc agctccttat ccttaggaaa
    15841 gtgggtatgc ccacatatca ccgtttggta gccttgctca gcacatgatt tcagcataga
    15901 cttttcatag cctatgaggg tacttctctc cctgtttagc ttttggttta tcgttttgta
    15961 caaaatgaag tcctttctcc cgaatattcc aagaagccca tttataaaag catcaatcaa
    16021 cgatagcatc ccttttccgg ctgcaccaat tttagccaac cattttgtca tgtatatgct
    16081 atggtcgaaa atatcgccat ggaggattag ggttttccct ccatcctgat ccaaaacaag
    16141 ccgatcaaca attttgagat ttcctgcttc aacagggcta aacttcctaa ggaactcatc
    16201 gtggttcccc gtaatgtagt aaaccttaac ccccttctcc atcatcttga taatctgcct
    16261 tacaaccttc aggtggcttt tgggaaagta atttctgctg aagcgccacg aatcaataat
    16321 atcaccgttg aggacaagca tctgcggctt aatagattta aggtacttca gaattttttt
    16381 tggtttgcag gcataggtag ccaggtgcag atcggaaatc acggctatgt ccaactgtct
    16441 atccttcatt tagctccagg tataaatttt atgcaaagga gcagtttagt tgtaaaatac
    16501 atgttactaa aacgttagga tttgaataat cctttgttac cactttacac cgcacaatta
    16561 ctatagggta gcattaacca tgcagggcgc attttgccat tataaaaaga ataaaaatgg
    16621 atggttgcca ccaccgacga tggatgaata tgaaaaaaaa ctattttagc cattcaaaaa
    16681 taggcgcaat gaaacaacct aagaagaaac gcgagtttcc gcatacctac gtcatcatct
    16741 tcacgcttat agttgtggca gcccttctca cctggtttat ccccggaggg gagttcaccc
    16801 gggaggtgaa gagtatcaac gggattgatc gcgaagtgat tgttcccggc tcctttcacc
    16861 aggttgacaa cagcccgcaa acatggcagg tgttcacctc catttttcag ggcatgaggc
    16921 gtacctgcga cattatcttc tacattctga taatcggcgg ttcgttttgg atgctaaacg
    16981 agagtaaagc gttggatgtg gccatcctgt cgtttctgaa ctttacccgg cgaatagaga
    17041 ggttcaagcc actgaaggca atcggggtga ataacctggt gatagcgctc atcatgatct
    17101 gcttcagctt ttttggggcg gtaataggga tgagcgagga gaccatcgcc tttgtggtaa
    17161 tttttgtgcc gctggccata agcatgggct acgactccat tgttggaata tgcctgtgct
    17221 ttctgggtgc gggtctgggt tttgccagcg cactgctaaa cccctttacc ataggaattg
    17281 cccagggact ttcgggaata ccgctgtttt cggggattga gtaccgattc ataatctggc
    17341 tggtgatcaa caccataggt attagctacg tgctatggta tgccaaccgc atcaggaaga
    17401 accccaagct atcgccagtt tacgagattg acgagcactg gcgccacaag gcttcagaag
    17461 agggggcaat cgttaaaacc aaagccggca aaatcgcatg ggttgtcttt gccgtggtag
    17521 ccattgtgct ggccattttc gcctacctct accccaccag ccagctggta gtcggccaaa
    17581 atgtcatttc ggttccggct attcccatag ccaccgtagt gtgggtcata ctagggcttc
    17641 tggcattaag gcactccgtg cagctgttca ttgtaaacat cctcatgttc accatcctgt
    17701 tcctcattgt tggcgttatg ggctatggat ggtacatcaa ggaaatagca tcgctattcc
    17761 tggtcatggg tattctggcc tctgctgcat ttggtaagaa cgccaaccag cttacacgct
    17821 cgttcattga tggggcaagg gatatcatgt cggctgcgct ggttgttggg ctggccagcg
    17881 ggattgtggt ggtgctggag caaggaaagg ttatagattc gctgctgaac tactcggcca
    17941 acgccatgca gggttacggg aaggtaacct ccatggccat catgtacatc ttctacaacc
    18001 tgctcaacct gattattgcc tccggatcgg ctaaggctgc cctcaccatc ccgctgatgg
    18061 cacaattctc cgacctcatc ggcataagca gacaggccac ggtaacggtt taccagctgg
    18121 gcggcggttt taccaacctt atcaccccca catcgggggt aatggttggc gtgctgagca
    18181 ttgcccgaat accctacaac aagtggttca ggtggttcct tccgctaatg ttcatcctga
    18241 ttgtagccgg gttcctgctg ctgctgccta cgcttttcct ccccctgagc gggttttaat
    18301 gccaaccttt gacttaatcg gtaggaaact ctggaaaatg acatatcgcg ctgcaggaac
    18361 gcctatacca gcaccgtaaa atccaaaatc cccggagcaa tatttggttg caccaactgt
    18421 ttaaaagttc cgcttgatgt tcatatcctg aacaacgctt acttgtaatc gtcagggttc
    18481 caaatta
//
LOCUS       DAOSHM010000047        18194 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_2495_length_18194_cov_9.599151, whole genome shotgun sequence.
ACCESSION   DAOSHM010000047 DAOSHM010000000
VERSION     DAOSHM010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 18194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18194
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2495_length_18194_cov_9.599151"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            567..1853
                     /locus_tag="PK839_07855"
     CDS             567..1853
                     /locus_tag="PK839_07855"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOG72684.1"
                     /translation="MKQQFRRFIHPRYTFLFLLFNVALNVYGQIYTGPIPKPTSGYGA
                     DGGYTVAIQHLSNPGFPDEDIRIYYPSGITSSVPTIFYSHGFGGSDPNNVLGLLSFVA
                     RKGYAIVFVPYQTLGVTTEQRYSTLLDGFIKAVHDFPNIIDTTKVGFMGHSFGGGATF
                     TNAYHCFTSLSWGQSGRFIFASAQYYSFNISQTELQSFPSNTKLLTMVYENDSTNDHR
                     MANDIFNTVNIPITEKDYLMVKSDTIDGYFYEAIHGVPNTLSSFDALDYFAIYRILDA
                     LCDYTFNGSAAGKEVALGNGSVNQVSMPGGMHNLVQTDYPNFAHPDTFYTYPCNLASN
                     PRQEYCNEPTSVGEHRNEKTFKIFPNPSHLSLTISFPSQNFSVEIFNYSGNLIYSKTD
                     IYQAVSIKTANFPNGLYLVRVTDADGNSWIKNFVKE"
     gene            2326..3726
                     /locus_tag="PK839_07860"
     CDS             2326..3726
                     /locus_tag="PK839_07860"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG72685.1"
                     /translation="MKEDYDSYLDEESEVELVGRFEENLRLNRSVFFDLHEFEGIIDF
                     YLAGDDRAKVRKAIEIAENIYPNSSEIQLKKAEFCFLEERYEDALHLLEFVENVDYHN
                     PELQYLKGEIFYELGDLNKSLQSFDKAIAVATDDRSEMMYRVSSFYLECDEVNLAIKY
                     LLKCHRYDASNLAVLFDLGYCYERNNELDKSISYYNAYLDIYPFSANVWYNMGIIYAK
                     QGKFDESLECYDFCIAVDPSYISAYHNKGNTLSALERYDGAIKVFTELLTLEPDDAGV
                     YSLLGECYERVNNLNMALSCYNKAISIEPDLAEGYFGIGVVMADRQKYDLSLDFIQKA
                     IALDSEQYDFWVGLGKLYYELNRIDEAIKAYREAITINPDEADGYLALAEALLYQEKF
                     SEVEVLVDELGGKFGSNATIKVINAAALYLSHRQKEALTILGDAKRLDPSAIEDFFSL
                     VSIVNDDDFIQKVNLL"
     gene            3976..4752
                     /locus_tag="PK839_07865"
     CDS             3976..4752
                     /locus_tag="PK839_07865"
                     /inference="COORDINATES: protein motif:HMM:NF014709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphosulfolactate synthase"
                     /protein_id="HOG72686.1"
                     /translation="MNYNLPFIPERPAKPRNVGLTMVMDKGLSLSETEGFIESSAEFV
                     DFVKLGFGTSLITNKLKQKIKLYQKGGIRPYLGGTLFEVFIIRGLFNDFVRFVSDLGL
                     DLVEVSDGSMKMDNKVKCDYISKLSEKFTVISEVGSKQADVIIPDDEWVAMMKREVEA
                     GSWKVIAEARESGNVGIYNADHSANVKLIEDITRHLKIENVIWEAPIKSQQAWFIKLL
                     GANVNLGNIAPNESIPLETLRLGLRGDTFFDFLPDNLKQQ"
     gene            4930..5907
                     /locus_tag="PK839_07870"
     CDS             4930..5907
                     /locus_tag="PK839_07870"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HOG72687.1"
                     /translation="MDLFGCRRPRLISVQLPVAIIIIHTLTLQQVVVDLAKRFISKVI
                     SRKSAAINCYFSAVVFALLALFVCHPTFAQVDRCSRDIPHLSANERITYKAYYNWKFI
                     WLRAADILFTSNDTSYNNVPAHHFLAEGRSLKEYDLFFKVRDRFESIVAKEGFRPLYF
                     IRDTYEGGYKAFNKYIYNYTGSSLYMETQTSERPFRIDIKPIKTCTFDVLSAIHYCRG
                     LDFSSHSKGDIIPFAIAIDNEVFELYIRYQGRERLELRDGRIYNTVKFSVKLVEGTVF
                     KGGEDMVVWASDDPGHVPIMVEAKILIGSVKAILSTAENLAFPLLFEKK"
     gene            complement(5942..7051)
                     /locus_tag="PK839_07875"
     CDS             complement(5942..7051)
                     /locus_tag="PK839_07875"
                     /inference="COORDINATES: protein motif:HMM:NF020965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HOG72688.1"
                     /translation="MVSCKIKTVILLVGMMPLGARVFAQPDSTFDSNARRSALETCLI
                     LGKIIPHDDTLNQLVNRNIKATEIQYSFKKSGRVIPYHQGLSLVIADLGNHDHLGYAF
                     GVIPNVSITTNTKNRVMVNLRLGLGAAWVTKKFSFASNIENVAISTNLNIMASLNANL
                     QIRITEKSKIYLGLNALHLSNGGFKKPNYGLNMLGASGGFSIAFSNKRECKVPKKSSD
                     DLQIETWPQIKLYVGMGVKETGDAGGPKYFPVSLQIAAARRVAKFVALGFGVDIAHDG
                     SSKFQIEQSNEVYTSPNDAFQVGASIYAELPLNRFSVFARQGFYLYNANPDLPFGYQN
                     IGFSYRIGHRVAIFISLKSHLNNADHIYAGLALSI"
     gene            complement(7134..9938)
                     /locus_tag="PK839_07880"
     CDS             complement(7134..9938)
                     /locus_tag="PK839_07880"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOG72689.1"
                     /translation="MDIKLKICISLLTFIFFWQNSVAQPITVVPALPTASDAVTVTFD
                     ATKASRTDLVGYSGDVYVHTGVAINGRKWQYVIGSWGINSTQPKLTRTGTNTYTLSIS
                     PSIREFYSVPADSAITQMCFVFRSADGSKQTEDIFYDVYEQGLSVSITSPLLLTPIYE
                     LNDNIQVNVSANNATSLTLYIDNQQEASTTENTLSYTYTATTYGKHWIKAVASNNDQS
                     VADSAYILVRSPLVTEELPAGMNPGVNITGEQTATVVLYDPQALKNYAYLIGSFSEWL
                     PDVQYYMKRTPDGKYFWTTLTNLSPDQEYAFQFLIDGYLRIADPYTHKTLDPNDQYIP
                     SSTYPGLMSYPTDKTYGIASVFSTTTNGYTWQTADFTPPSKENLIIYEMLIRDFVSDS
                     YIQTAMDSLSYLKKLGVNAVELMPISEFEGNDSWGYNPSFYFAPDKAYGKANDYKAFI
                     DEAHQQGMAVIMDIVLNHSFGQSPLVQLYSNSNGVTLGEPTTDNPWYNQTCPHQPYCW
                     GYDFNHESPETRKFVDSVLTYWLTEYRVDGFRFDFSKGFTNTPNAGSAYDQPRIDNLK
                     RIADKIWTINPDAYVILEHFCDNSEEKVLAEYRNAEGKGMLVWGNLNYNYGEAAMGWL
                     DNSNFSAVAYKQRGWSVPNLVGYMESHDEERLMYKNLTFGNTSNPSHNVRNLPIALKR
                     MELAANFFIPIPGPKMIWQFGELGYDISIDYNGRLGRKPLHWEYYTQTERRNLFNIYA
                     NLNRLKQEFDVFTTSSYEYMLSGSKKWIKLDGTDMDVVVMGNFDVNGVSFSIDFPATG
                     KWYEYYTGDSIELSTTNATFSMAPSEYRLYTSVKVNRDDIFVGVEENPDETSERVHLW
                     PNPSNGEFQLSFTLPKALEVQLELYNLMGQKVYHVQHQGILGANRLKVDLPPEIVTGL
                     YIFRINSQLYNQTGKLSIVK"
     gene            complement(10130..11956)
                     /locus_tag="PK839_07885"
     CDS             complement(10130..11956)
                     /locus_tag="PK839_07885"
                     /inference="COORDINATES: protein motif:HMM:NF027726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:2001070 - starch binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusF/SusE family outer membrane protein"
                     /protein_id="HOG72690.1"
                     /translation="MKLKSIYKLAALLFTATMLFFACTEESSDVRLDPKLSTSQVIDI
                     TDSSATVVGFVVAAGDGYTERGVCYNTQPNPTTSDTKVVFNEDSVKGATFSVTITGLH
                     YTTKYYARAYAINVSGTLYGEELSFTTPVALPKLTTTAITEITGSSATSGGNVIDAGG
                     ADVTARGICFATHESPTIKDSITKNGDGLGEFTSGLTNLIGNTTYYVRAYATNSAGTG
                     YGPQISFTTPVDIPRVITESVSEITKTTAVTGGNVTYDCGAEVTEKGIVLSTSPNPTT
                     ADTKFTADPPTGTGQFIINLEGLTQFTTYHVRAYAINSKGTAYGEDISFITLPDITKF
                     WVVGDYNGWDNSDNAKYIISTTSSGGLAEGYVYLTSGGIKLTTDHSWDNAHTFGDDGT
                     GGLTNPGSNIPVPSDGYYRIRANLNDMTYSLLLTSWGIIGDATPLGWGDESPLEYKAA
                     LEQWIGAYHLTAAAFKFRANHSWDYNYGLNAGDNFLDAGGDNIPITMEDDYAITLDLS
                     HPNEYTFSANRWGIIGGSVPPYDWSVDVNLTWDGVNKVFTKTLDLKAGDFKFRANDGW
                     DVNFGGDLSALTQGGANIPIAEDGNYTITLDPWNKVGTVTKN"
     gene            complement(11979..13028)
                     /locus_tag="PK839_07890"
     CDS             complement(11979..13028)
                     /locus_tag="PK839_07890"
                     /inference="COORDINATES: protein motif:HMM:NF025650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusE domain-containing protein"
                     /protein_id="HOG72691.1"
                     /translation="MKRNSLIYIGFIGLLGILSGCEKDETKVTMLDEPNPPVLVSIPD
                     NLILNRSAANDTVEFTGIPVDPGFEASATYSLETCEAGTNFADPLTLYSGSKVNSIKF
                     KISNLNELLLRKYRGDVEVQAEFRIRAVLTVDAGTGAPGTGSNPFLYTSESKTATVSP
                     YGLPRLDVMDGTTVLGKIESAAGDGNYSGFVKLDVAKPFTLYDPDADITYGSNDAGTA
                     LEVNGSAIVPPANGWHVVSANTNDLTYGMDAHMISIVGSALTGNEDGWNQDRMMEYDY
                     DKHYWYITTDLYVGFFKFRKNSSWSWNMGLSDVEGELQQGGVGNDIPITSAGNYSITF
                     TILSDEKGTYTIVKN"
     gene            complement(13056..14696)
                     /locus_tag="PK839_07895"
     CDS             complement(13056..14696)
                     /locus_tag="PK839_07895"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOG72692.1"
                     /translation="MKSRKLILGLLLPAMIFSSCVGDLDVNPKDPNNIMAGNLGDDPV
                     NIKKALGKIYASFIIAGQGSVTGGGADISASDENFFTTTRALWNLQEITTDEAICAWG
                     DVGIADLNTQTWSAQNPFLTALYQRLVLSITYANSFIKVTSGNPDPDVQRYVAEARFL
                     RALAYYYLMDMFGNPPFTTEADEVGKFYPRQLDPNVNVARPMLFNYIVSELKAISPVL
                     AAPGGVSTQANRAAAWMLLARTYLNAQVYTGTAKWDSCQMYCDSVINSGAYSLATNYR
                     QNFSADNTYPTNPEMILAWEQDGMYTQGYVGTTFIIESSSDATYIRAEDYHGLTSNTN
                     WNGNRARLKFAETILGVSNPGWAEGTELSTLYPDDSRIFLKVKKTTDIPSASSSGDYG
                     IGVYKITAKNSDGTRPPSANPAYADTDFPIFRLADAYLMRAEAKFRLGEATALADLNY
                     VRDRAYHAGEFGSNTPNRLSTLTAQDIINERGREFYYEAQRRTDLIRFGQFTGSDYLW
                     EWKGGVYEGTSTADYLNIFPLPAVEISTNPYIHQNPNY"
     gene            complement(14717..17680)
                     /locus_tag="PK839_07900"
     CDS             complement(14717..17680)
                     /locus_tag="PK839_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009122417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOG72693.1"
                     /translation="MKNKRIFMKICFILVLVFYQGLANGQDITVTGTVTDNANNEPLP
                     GVAVVVKGTTIGTATDLNGKYSLRTQANAVLVFSYVGYEKQEIPVNGRSVVDVALKTS
                     SEQLEEVVVIGYGTTKKADLTGSVTTVSSKDFNKGVISSPQELLVGKTPGVVITTSNG
                     APGSGSTIKVRGGSSLYASNSPLVIVDGVPLDNNNVSGSSNPLSFVNPNDIETITILK
                     DASSTAIYGSRASNGVILITTKKSKEGASFRVNYNVTTSIASAIKLMDVYSGDQMRQI
                     AYDHMGTLYNPSTLTLLGSKNTNWQNEIFRNAFSQDHNVSVSGAYKILPYQVSVGYTN
                     QDGIMKNTNMERLTGAISLNPSFLNNSLNVTVNAKGMTTKQNYGDYGALGSAINMDPT
                     QPIYDGNPASAGYFQWANYGANLGTPNPVEQLMAADNKADVNRFIGNIQLNYKFPFLQ
                     ELQANLNMATDYTNSDGHNNRPVTSPSVLIAPLAWGKLNDYSATNKNNLLDFYFNYTK
                     DLTQIKSKVEAMAGYSWQHFEREGDSYTRGVVDVDHPYQMSDSSSFITENYLVSFFGR
                     LNYTLMDRYIITATLRDDGSSRFSEDNRWGLFPSAALAWKVKEESFLKNVNFVSDLKL
                     RASWGVTGQQDIGSDYPYQAQYVASAIGSWYPIDGTFLPTLRPNAYDPDIKWEETTTQ
                     NIGLDFGFLNNRISGSVDLYMRKTKDLLNTIDIASGSNFKNTLLTNVGNMENKGVEVA
                     LNLIPISQQDMTLTLGFNLTYNKNEVTKLLMNDDPNFIGILYGDAFTGQKQVTRVGYP
                     VYSFFVNKQVYDAMGNPIEGLYVDLSGQGGVVNGDNADKYVYKNPAPDYLMGFSFRFN
                     YKNFDLSASTRASIGNYVYNGVAAGASYDQMQQIGYWKNFTTQLSDTRFVKRQFTSDY
                     FVENASFFKLDYVTAGYNFNNIMDKFNIRVNFTVQNAFIITKYNGMDPEVDGGVDNSF
                     YPRPRTFLLGLSFTF"
BASE COUNT         4808 a         3868 c         4251 g         5267 t
ORIGIN      
        1 tagctcaccg tcgcgggcat cgagcgactt gtaggtgtcc agaatggcat cgtagccgat
       61 aaacccaaat actttagcct ctacctgatg tttctcagca tcctgagaaa tggcagcatt
      121 tatttggaga attagtagaa gggcaaacag tagaattcgt ttcacaggaa atggagaatt
      181 ttagtatttc agttcaactg aaaaatggtt tatcttaggt gaatactcaa atttctgggc
      241 aaatttgtag aaactttact aaacaaacaa caaatatggg tagtccaact ggaatgcttg
      301 ccataaggta taaaaatata aaaagatgat gggttgatta atttttcagg gataaactag
      361 ccaatgagct ggctgaaccg taaactaggt gtggagttgg tcaattttta gttattacag
      421 cctaaacaaa ttaaccccat aagcgtttaa cgttaagaaa atttgacaaa gattggattg
      481 tttaataacg ggccgtcgcg attttaagaa tagcaggaga tgacagctga aatacgaata
      541 tcagaatact cttaataggg aatgttatga aacagcagtt tagaagattc attcatccta
      601 gatatacatt tctttttcta ctatttaatg tggccttgaa tgtatatgga caaatttata
      661 ccgggcctat tcccaaacct actagcggat atggcgctga tggtggttac actgttgcga
      721 ttcagcatct ctctaacccg ggttttcccg atgaggatat aagaatctac tacccttctg
      781 gtatcacttc aagtgtgcct acaatatttt atagtcatgg ttttggaggt tctgacccta
      841 ataatgtttt aggattgctt agctttgtag ccaggaaagg gtatgccatt gtttttgtgc
      901 cctaccaaac gctaggtgtg actactgagc aaaggtatag taccctttta gatggtttta
      961 ttaaagccgt acatgatttt cctaatatta tagacaccac aaaagttggt tttatggggc
     1021 actcatttgg tggtggtgcc acctttacca atgcctacca ttgttttact agtttgagtt
     1081 ggggacaatc ggggcggttt atttttgctt ctgctcaata ttactcattc aacatctcgc
     1141 agaccgaatt acagtctttc ccatcgaata ccaaactcct caccatggtt tatgagaacg
     1201 attccacaaa cgaccaccgc atggcaaatg atatttttaa taccgtcaac attccgatta
     1261 ctgaaaagga ctatcttatg gtgaagtccg acacaattga cggatacttt tatgaggcaa
     1321 ttcatggcgt tccaaatact ctttcttcct ttgatgccct tgactacttt gccatatatc
     1381 gtattcttga tgccttgtgt gactatacat ttaatggaag tgctgccggt aaagaagtag
     1441 cattgggtaa tggtagcgta aatcaggtat caatgccagg cggaatgcac aatttagtgc
     1501 aaaccgacta ccctaacttt gcacatcccg atacctttta tacctatccc tgtaatttgg
     1561 cgagcaatcc cagacaggaa tactgcaatg aacccactag tgttggagaa catagaaatg
     1621 aaaaaacatt taaaattttt cccaacccct cccacttatc cctgaccatt tcttttcctt
     1681 cacaaaactt ttctgttgaa atctttaact attcgggtaa tttaatctat tcaaaaaccg
     1741 acatttacca ggccgttagt ataaaaacag cgaatttccc taatggtctt tacctggtca
     1801 gggttactga tgctgatggg aactcgtgga taaaaaactt tgtaaaggag taattcctac
     1861 taatctaaaa ctatggctga ctacccggtt attctaccaa atgtacccat agcattaact
     1921 ggactagtga ataacatact acaagttaac cgttagattt gtttttgctg tgattatgca
     1981 aattttttac ctggcatttc ctgctaccct tacaaaattt gttaatgatt gtcttgaatc
     2041 cagttttact cctgaatgtt gcatttgccg taggcctcag cagctgcttg cattgcatta
     2101 aatggtgggt ttaagtcagc aggagcaggt aacatcttta ttaccgggca gaaggccact
     2161 cttcaaatgc atcgtttagc caacttaggt ggtgatattt atacccacac aagttggatg
     2221 ttgaactaaa cgatgggagg ggcattgtta gctaaattta atgtggagct tagttaagtt
     2281 ttcgttaaaa tgactatgtt tgtagttaga atgttatttt aaacaatgaa agaggactac
     2341 gatagctact tggatgagga gagcgaggtt gagctggtgg gccggtttga ggagaacctg
     2401 cgacttaaca ggtctgtatt tttcgatcta catgaatttg agggtattat agacttttac
     2461 cttgccggcg acgatcgggc aaaggttagg aaggccattg aaattgccga aaatatatat
     2521 cccaactctt cggaaattca gctaaaaaag gcagagtttt gtttccttga ggaacgatac
     2581 gaggatgccc ttcatttact ggagtttgtt gagaacgttg attatcataa ccccgaattg
     2641 cagtatctca agggcgaaat attttatgag ctgggtgacc tcaacaaatc cttacaaagc
     2701 ttcgacaagg ccattgccgt tgcaaccgat gaccgctccg aaatgatgta tcgagtttca
     2761 agtttttact tagaatgcga tgaggtaaac cttgcaatca aatacctctt gaagtgccat
     2821 aggtatgatg ccagcaacct cgcagtgctc ttcgacttgg gttactgcta cgagcgcaat
     2881 aacgagctgg ataaaagcat tagctactac aacgcctacc ttgatatata cccattctca
     2941 gctaatgttt ggtacaacat gggcataatt tacgctaaac aggggaagtt cgatgagtct
     3001 ttagaatgct acgacttctg cattgcggtt gatccctcct acatttccgc ataccataat
     3061 aagggtaata cactctcggc cctagagcga tacgatgggg cgataaaggt ttttaccgag
     3121 ctgctcaccc tggaacccga tgatgctggt gtttactccc ttttgggtga gtgctacgaa
     3181 cgggttaaca accttaatat ggctttaagc tgctacaaca aggccatatc aattgaaccc
     3241 gatttggccg aggggtactt tggaattgga gttgtaatgg ccgaccgcca gaagtacgat
     3301 ttaagccttg acttcattca gaaggctatc gctttggact ccgagcagta tgacttttgg
     3361 gttgggctgg gaaaactcta ctacgagctc aatagaattg atgaggcaat aaaagcatac
     3421 cgcgaggcaa ttacaatcaa tcccgatgag gctgatggct atcttgcact ggccgaagcg
     3481 ctgctatacc aggaaaagtt ctcggaggtt gaggtgctgg ttgacgagct gggtggcaag
     3541 tttggctcaa atgccaccat taaggtgatt aatgccgctg ctctctacct ttcgcatagg
     3601 caaaaggagg cattaaccat tttgggcgat gcaaagcgtc tagacccctc tgcaattgag
     3661 gattttttta gcttggtttc tattgtcaac gatgatgatt ttattcaaaa ggtgaacttg
     3721 ctttagggtg tcagctctct acgatgaacc cggttaactt tttaaataat gatgcgatac
     3781 taactgctgc ggtggatagc attgaaaagc acaaggtgga tgtaaaagaa ataataagag
     3841 ctatgtgcgt tccatagctg attaatggaa gttgaaaatg ccactagtta atgaggccgc
     3901 tggtttgaat atgccaggca aggaaagtaa attttgaact tttcgaatct aaaccctaat
     3961 aaaaaataaa gtactatgaa ctacaatctt ccctttattc cggaacggcc tgccaagccg
     4021 cgcaatgtgg gattaacaat ggttatggac aaaggcttaa gcctatccga aaccgagggc
     4081 tttattgaat cgagcgctga atttgtcgat ttcgtcaagc tgggatttgg tacctcgctc
     4141 atcaccaata agctgaagca aaaaatcaag ctataccaga agggtggcat tcgtccctac
     4201 ctgggaggaa ctctttttga agtattcatt atcagggggc tctttaatga ctttgtccgc
     4261 tttgtcagcg acctgggtct tgacctggtg gaggtttccg atggctccat gaagatggat
     4321 aataaggtga agtgcgacta catttccaag ctgtccgaga aatttaccgt gatttctgag
     4381 gtgggttcca agcaggccga tgtaatcatt cccgatgatg agtgggtagc catgatgaaa
     4441 agggaggttg aagccggcag ctggaaggta atagctgagg cccgagaaag tggaaatgtg
     4501 ggcatatata atgccgatca ttcggcaaac gtgaagctta ttgaggatat caccaggcac
     4561 ctcaaaattg aaaatgttat ctgggaagcc cctattaaaa gccagcaggc atggtttatt
     4621 aaacttctgg gcgctaatgt aaaccttgga aatattgctc ccaacgagtc aataccactc
     4681 gaaaccctta ggctcgggct tagaggcgat acctttttcg actttctacc cgataacctt
     4741 aagcagcaat agcccaactt aagtagatct ttggtaaata tttaatccca atgataaggt
     4801 catacaagaa aggataggat catgcactta ttgtatggcc tgcattggaa ttatgggagc
     4861 ctggtggatg ttatagggta aatgattgtc ctttgtctgt tgatttaaaa aatttgcagc
     4921 tgaagttcca tggatttatt tggctgcagg cgcccaaggc taatcagcgt tcagctacca
     4981 gttgccatta ttattattca tacgctaaca ctccaacaag tagtagttga cttggcaaaa
     5041 cgattcatct caaaagtaat ttcaaggaaa tcagcggcta tcaactgcta tttcagtgca
     5101 gtggtgtttg cattgcttgc actttttgta tgccacccca catttgccca ggttgataga
     5161 tgctccaggg acattccaca tctcagcgcc aacgagcgca tcacctacaa ggcttactat
     5221 aactggaagt ttatatggct tagagcagcc gacatccttt ttacctcaaa cgatacctcc
     5281 tataataatg tgcctgcaca tcactttctt gccgaagggc ggagcctaaa ggagtacgac
     5341 ctttttttca aggttcgcga caggttcgaa tccattgtgg ccaaggaggg ttttcgaccc
     5401 ctttacttca ttcgcgacac ctacgaggga ggctataaag ctttcaacaa gtacatctat
     5461 aactataccg gcagctcctt gtacatggag acccagacat ccgaacgacc cttcaggatt
     5521 gatattaaac ccattaaaac atgcaccttc gatgttttaa gcgccattca ctactgcagg
     5581 gggctggatt tttcgagcca tagcaagggc gacataatac cctttgccat tgctattgac
     5641 aacgaggtat ttgagctata tattcgttac cagggaaggg aaaggctgga gttacgcgat
     5701 ggaagaattt ataatacggt gaaattcagc gttaagctgg tggagggaac ggtttttaaa
     5761 ggaggtgagg atatggtggt ttgggccagc gacgaccctg gacatgttcc aatcatggtt
     5821 gaagcaaaaa ttctgattgg ttctgttaaa gcaatactca gcactgccga aaatctggca
     5881 ttccccttgt tattcgaaaa aaaatagctt gctgcatagt ccacagttgg gcaaatacct
     5941 ttcagatgct taaggctaac ccggcataga tgtggtcggc attgttaaga tggcttttaa
     6001 gcgatataaa aatggctact ctatggccaa tcctgtagct aaatcctata ttctggtagc
     6061 cgaatggaag gtcggggttg gcgttataga ggtagaaacc ctgccgggca aaaaccgaaa
     6121 acctgttaag cggcagctcc gcataaatgc ttgcccccac ttgaaaggca tcgttgggcg
     6181 aggtgtaaac ttcattcgat tgttcaattt gaaacttcga tgagccatcg tgcgctatat
     6241 caaccccaaa accgagggca acaaatttgg ctaccctacg tgctgcagct atttgtagcg
     6301 aaacaggaaa gtacttaggt ccaccggcat cgcctgtttc ctttactccc atgccaacat
     6361 aaagcttaat ttgaggccag gtttctattt gcaaatcatc agaacttttt ttcgggactt
     6421 tacactccct cttgttactg aaagctatgc taaaaccacc gcttgcccca agcatgttca
     6481 acccgtagtt tggctttttg aacccgccat ttgacaggtg aagagcattt agtccgagat
     6541 aaattttcga tttttctgtt atcctgattt gcaggttggc attcaggctg gccataatgt
     6601 taaggtttgt ggatatggca acgttttcaa tattcgatgc aaagctgaac ttttttgtta
     6661 cccatgcagc acccaggccc aaacgaaggt taaccatcac ccgatttttt gtgttcgtgg
     6721 ttatggaaac attgggtatt acgccaaagg cgtagcccag gtgatcatgg ttaccaaggt
     6781 cggcaataac cagcgacaat ccctggtggt aggggattac ccttcctgac tttttaaagc
     6841 tatactgtat ctctgttgct tttatatttc tatttaccag ctggttaagg gtatcgtcat
     6901 ggggaatgat tttcccaagt atgaggcatg tttccaaagc gcttcttctt gcgttgctgt
     6961 cgaaagtgct gtcgggctga gcgaatactc ttgcgccaag gggcatcata ccaacaagta
     7021 gaataacggt ttttatcttg cagcttacca tacctcaaaa atagtaaacc cgtccgaatt
     7081 tttctcaacg gacgggtttg atgcacctcg taaacctaaa ccgaaaaaga atcctatttg
     7141 acgattgaaa gttttcctgt ttggttatat aattggctgt tgatgcggaa tatgtataga
     7201 cccgtaacaa tttctggcgg caaatcaacc ttcaacctat tggcccccag aattccctga
     7261 tgttgtacat ggtaaacctt ttgacccatc aggttgtata gctccagctg aacttctagc
     7321 gcttttggta gggtgaatga gagttgaaat tctccattcg atggattagg ccacaaatga
     7381 accctctcag aggtttcatc cgggttttct tccacaccaa caaatatgtc atcgcggttc
     7441 acctttacgg aggtgtagag cctgtattcc gagggtgcca tgctaaaggt ggcattggtt
     7501 gtggagagtt caatggagtc gcctgtgtag tactcatacc acttaccagt cgctgggaaa
     7561 tcaatggaga agctaacccc attcacatca aagttaccca ttaccaccac gtccatgtcg
     7621 gttccatcca gcttaatcca ttttttagag ccggagagca tgtactcgta actactggtc
     7681 gtgaatacgt caaactcctg ctttagcctg ttgaggttgg cgtatatgtt gaaaaggttg
     7741 cgtcgctcag tttgggtgta gtactcccag tgtagtggtt tacggcctaa gcgaccatta
     7801 tagtcaatgg agatatcgta gcctagctcg ccgaattgcc atatcatctt ggggccaggg
     7861 atggggataa agaagtttgc tgcgagctcc atacgcttaa gggcaatagg gagatttctt
     7921 acgttgtggc tgggattgga tgtgttgcca aaggttaaat ttttatacat caaccgttcc
     7981 tcgtcgtggc tttccatata gccaaccagg ttgggaacgc tccaccccct ctgcttatag
     8041 gcaactgctg agaaatttga attatcaagc caacccatag ctgcctcacc atagttgtag
     8101 tttaaattac cccacaccag cattcccttc ccttcagcgt tgcggtactc ggccaaaact
     8161 ttctcctcgg agttgtcgca aaagtgttca aggataacgt aagcatcggg gttaatcgtc
     8221 catatcttgt cggcaatacg cttcaggttg tcaattcggg gctggtcgta tgccgagccc
     8281 gcgttgggcg tgttggtgaa accctttgag aaatcaaatc ggaatccgtc aaccctatac
     8341 tcggttagcc agtaggtgag gaccgagtca acaaattttc gtgtttcggg gctttcatgg
     8401 ttaaaatcgt agccccagca gtagggttgg tgcgggcagg tttggttgta ccacgggttg
     8461 tcggttgtag gttcaccaag tgtaacacca ttggagttgg agtacagctg cactagcggt
     8521 gattggccaa aggagtggtt taggacaata tccataatta ctgccatgcc ttgctggtgg
     8581 gcttcgtcaa taaaggcctt gtagtcgttt gctttgccat aggccttatc aggggcaaag
     8641 tagaatgatg ggttgtatcc ccagctgtcg ttgccctcaa actcgctgat gggcataagc
     8701 tcaacggcgt taaccccaag ctttttcagg tagctgagtg agtccatggc cgtttgaatg
     8761 tagctatcgg aaacaaagtc cctgatcagc atttcgtaaa ttattaggtt ctcctttgaa
     8821 ggtggggtga aatctgctgt ctgccaggta tagccgtttg tagttgtgct gaataccgaa
     8881 gcaattccgt aggttttatc ggtggggtag ctcatcagcc cagggtaggt agaggagggg
     8941 atgtactggt cgttggggtc aagggtcttg tgtgtatagg gatcggcaat tcgcaggtag
     9001 ccgtctataa ggaattgaaa agcatactcc tgatcggggc tgaggtttgt aagggtagtc
     9061 cagaagtatt ttccgtcggg tgttcgcttc atgtagtact gtacatcggg aagccattcg
     9121 gaaaaagaac caataaggta ggcgtagttt ttcaatgcct gcggatcata aagcacaaca
     9181 gtggcggttt gctcaccggt aatattaaca cccggattca taccggctgg gagttcctcc
     9241 gtaaccagcg gtgatcgaac cagaatgtaa gccgaatcag caaccgattg gtcattattg
     9301 ctggcaacag ccttaatcca gtgtttaccg taggttgttg cggtataggt gtaacttagc
     9361 gtgttttcgg tggtggaagc ttcctgctgg ttgtcaatgt aaagggttaa gctggtggcg
     9421 ttgtttgcgg agacattaac ctgaatgttg tcgtttagct cgtaaatagg cgtgagaagc
     9481 aagggtgagg ttatgctaac cgataatccc tgttcataaa catcataaaa aatatcctcg
     9541 gtctgctttg agccatcggc gctacgaaac acaaagcaca tctgggttat tgctgaatcg
     9601 gcaggaacgc tgtagaactc acgaatgctg ggtgaaatgc taagagtata ggtatttgtt
     9661 cccgtgcggg ttagctttgg ttgagttgag ttgatgcccc agctaccaat aacatactgc
     9721 cattttcttc cgttaatcgc aacgccggtg tgaacataaa catcaccgct gtagcctacc
     9781 aggtcggttc tgcttgcttt tgtagcatcg aatgtaacgg taacggcatc cgaggcggtg
     9841 ggtagcgctg gaacaactgt gatgggctgg gcaacggaat tttgccaaaa aaatataaaa
     9901 gtcagcagcg aaatgcaaat tttaagcttg atatccatag cagtgtggct ttatttacag
     9961 gttgaaaacc ttacaggatg gaatattatt gaaatacata aatgttttta acaggggtaa
    10021 agctgcattt tgtgttcaca aatttccaag gtgagttttt ttaatgctgt tgctttaaaa
    10081 agtaaaaaag gctgccccat ttccaggggc agcccttcat cgaaactgtt tagtttttag
    10141 taacagttcc caccttgttc caaggatcca gcgttatggt gtagtttccg tcttccgcaa
    10201 tgggaatatt ggctccgccc tgcgtaagag ctgacaggtc gccgccaaag ttaacatccc
    10261 aaccatcatt tgccctgaac ttaaaatccc cggcctttag gtcaagtgtt ttggtgaaca
    10321 ccttgtttac cccgtcccag gtcaagttca catccacact ccaatcgtat ggcggaacag
    10381 aaccaccaat aattccccac ctgttagcac taaaggtgta ctcgttgggg tggctcaggt
    10441 cgagtgttat ggcgtagtca tcctccatag ttattggaat attatctccg ccggcatcca
    10501 ggaaattatc gcctgcgttt agcccgtagt tgtaatccca gctatggttg gcacggaact
    10561 tgaaagctgc tgcagtgaga tggtaggctc caatccactg ttcaagggca gctttgtact
    10621 caagtggaga ttcatcgccc caaccaagcg gagtggcatc accaataata ccccagctgg
    10681 tgagaagcag cgagtaggtc atatcattca ggttggccct aatccggtag tagccatctg
    10741 agggcacggg aatgttactt ccaggattgg taaggccacc ggtgccatca tcaccaaagg
    10801 tgtgggcatt atcccagctg tggtcggtgg tgagtttaat tccaccggag gtgaggtaaa
    10861 catagccctc agctaatccg ccacttgaag tagtcgaaat gatgtacttg gcgttatcgc
    10921 tgttatccca accattgtag tcgccaacaa cccagaactt ggttatgtcg ggcaatgtta
    10981 tgaacgaaat atcctcgccg taggccgtac ccttgctgtt gatggcgtaa gccctaacat
    11041 ggtaggtggt gaattgggtc aacccctcaa ggttgatgat aaattgacca gtaccggttg
    11101 ggggatcggc agtaaatttg gtatcagcag tggtgggatt gggtgatgtg ctcagaacaa
    11161 tacccttttc ggttacctca gcgccgcaat cgtaggttac attaccgcct gtaaccgcag
    11221 tggttttagt tatttctgat actgattcgg ttatcacgcg gggtatatca acaggcgttg
    11281 tgaatgaaat ctgggggcca tagcctgttc ctgcactatt ggtagcataa gccctcacgt
    11341 agtaggtcgt gttgccaatc aggttggtca acccgctggt gaactctcct aaaccatctc
    11401 catttttagt tatgctatct ttaatagttg ggctttcgtg agtggcaaag caaatgccgc
    11461 gggccgtaac atcagcacca ccagcatcga taacgtttcc accactggta gcagagctac
    11521 ccgttatttc agtaattgca gtggtggtta gcttgggaag agcaacaggg gttgtaaacg
    11581 aaagttcttc accataaagg gtaccgctaa catttatggc gtaggcacgg gcatagtact
    11641 tggttgtata gtgaaggccc gtaatggtaa cgctaaaagt tgctcccttt acgctatcct
    11701 cgttaaaaac aaccttagta tcactggttg ttgggttggg ctgcgtgttg tagcacactc
    11761 ccctttcagt gtaaccatca cccgcagcca caacaaaacc aacaacagtt gctgagctgt
    11821 ctgtaatgtc aataacctgc gaggtggata gctttggatc gagtctaaca tctgaggact
    11881 cctccgtgca ggcaaaaaat agcattgttg ccgtaaaaag gagtgctgct agcttataaa
    11941 ttgatttcag cttcatatgg tatgcgaatt ataatcattt aatttttaac aattgtatag
    12001 gttccctttt catcgcttag tatggtgaag gtgatggagt agttacctgc tgatgttatg
    12061 ggaatatcat ttccaacacc accttgttgc aattcaccct caacgtcgga cagacccatg
    12121 ttccatgacc agctggagtt ctttctgaat ttaaagaatc caacatagag gtcggtggta
    12181 atgtaccagt aatgtttgtc gtaatcatat tccatcatcc tatcctggtt ccatccatct
    12241 tcattaccgg ttagggcaga acccacaatg cttatcatat gagcgtccat gccataagtc
    12301 agatcgttgg tattggcaga tactacatgc cagccattgg cagggggaac aattgcagaa
    12361 ccgttgactt ccaaagcagt accggcatca ttgctgccat aggttatatc ggcatcagga
    12421 tcatacaggg tgaagggttt ggctacatcc agcttaacaa atccggagta attcccatcg
    12481 ccggcagcag attctatttt acccaacacg gtagtgccat ccataacatc aagtctgggt
    12541 agcccatagg gggaaacagt ggctgtttta ctttcagagg tgtaaaggaa cgggttactt
    12601 ccagtaccgg gagcacctgt tcctgcatca acggtgagca cggcgcgaat cctgaactct
    12661 gcttgaactt caacgtcacc acggtatttt ctcaggagca gctcgttaag gttgctgatc
    12721 ttaaatttga tgctgttaac ctttgacccg gagtaaaggg ttaaaggatc ggcaaagttg
    12781 gtgcccgctt cacatgtctc caatgaatag gtggcagagg cttcaaaacc tggatctaca
    12841 gggattcccg taaattcaac tgtatcgttt gccgctgacc tgtttaaaat taggttatcc
    12901 gggatggata ccagaacagg gggattaggc tcgtcaagca tggtaacttt tgtctcatct
    12961 ttttcgcatc ccgacaaaat accaagcaag cctataaatc ctatgtatat taatgaattt
    13021 cttttcattg tgcttaattt attgagttag tagtgctagt agttaggatt ctggtggata
    13081 tatggatttg ttgatatctc aactgctggc aacgggaaga tgttcaggta gtcagctgtg
    13141 ctggttccct cataaacgcc acctttccat tcccagaggt aatcgcttcc ggtaaactgg
    13201 ccaaaacgaa ttaggtcggt tcggcgctga gcctcgtagt agaactccct accgcgctcg
    13261 ttgatgatgt cctgagcagt caaggttgac aggcggttgg gggtgtttga accaaattcg
    13321 cctgcatggt aggctctgtc cctaacgtag ttcaaatcag caagagcagt tgcttctcca
    13381 agcctgaact tagcttcagc cctcatgagg taggcatcgg ccagacgaaa tattggaaaa
    13441 tcggtgtcgg cgtaggccgg attagctgat gggggacgtg ttccatccga attcttggct
    13501 gtaattttgt aaactccaat accataatcg ccacttgatg atgcgcttgg tatatcggtg
    13561 gtttttttca ctttaaggaa aatacgtgaa tcatcaggat aaagggtgct tagttctgtc
    13621 ccttctgccc atcccgggtt gctaactcct agtattgttt cagcgaattt aagacgtgca
    13681 cggttcccat tccagttggt atttgaggtt aaaccgtggt aatcttccgc tctgatgtag
    13741 gttgcatcac tcgacgattc gattatgaag gtagtgccca catagccttg cgtgtacatt
    13801 ccatcctgct cccaggcaag tatcatttca gggtttgtag ggtaggtgtt atcggcagaa
    13861 aaattctggc ggtagtttgt agccagcgag taggctccag agttgattac gctatcgcaa
    13921 tacatttggc agctatccca ctttgccgtt ccggtgtaaa cctgagcatt caggtaggta
    13981 cgggctagca gcatccatgc agcagctctg tttgcctgtg tggatacgcc tccgggagca
    14041 gctagaactg gagaaattgc ctttagctcc gaaacaatgt agttgaacag catgggcctg
    14101 gcaacattca catttggatc gagctgccgg gggtagaatt tcccaacttc atcggcctca
    14161 gtggtaaagg gaggattccc aaacatatcc atcaggtagt agtatgccag agcccgtaaa
    14221 aatctagctt cagccacata gcgctgaaca tcggggtcgg gattcccgct ggtaacctta
    14281 atgaaactat ttgcataggt tatggatagc accagacgtt ggtagagtgc ggtaagaaat
    14341 gggttctgcg cactccatgt ctgggtgttc aaatcggcaa tgcccacatc accccatgca
    14401 caaatagctt cgtcggttgt gatctcctgc aggttccaaa gagcacgggt agtagtgaaa
    14461 aagttttcat ctgaagcgct aatatcggct ccacctccgg taacgcttcc ctgtccggca
    14521 atgatgaagc tggcgtaaat cttgccaagt gctttcttaa tgttaacggg atcatcacca
    14581 aggttccctg ccataatatt atttggatcc ttggggttaa catccaaatc tcccacgcat
    14641 gaactgaaaa tcatcgccgg aagaagcaat cctaatatca atttcctgct tttcatattt
    14701 gtagtttttt tgagttttag aaagtaaagc ttaagccaag caggaaggta cggggacggg
    14761 ggtagaaact gttgtcaact cctccatcta cctcgggatc cattccgttg tacttggtta
    14821 tgataaatgc attctgcacg gtaaagttaa cccgaatgtt gaacttgtcc atgatgttgt
    14881 tgaagttgta tccggcagta acatagtcaa gcttgaagaa cgatgcgttt tcaacaaagt
    14941 agtcgctggt aaactggcgt tttacaaacc tggtgtcgct aagctgggtg gtaaagtttt
    15001 tccagtaccc aatttgctgc atctggtcgt atgaagcgcc ggcagctacg ccgttgtaaa
    15061 catagttgcc tatgctggct cgagttgatg ccgataaatc aaaattctta tagttgaacc
    15121 ggaaggagaa gcccatcagg taatcgggtg cgggattctt gtagacgtac ttgtctgcat
    15181 tatcaccatt tactactccg ccttggcccg aaaggtcaac gtataagcct tctatggggt
    15241 tacccattgc atcatacacc tgtttgttta cgaagaatga gtaaaccgga tagccaactc
    15301 gggttacctg tttttgcccg gtaaaagcat ccccgtaaag aattccaatg aagttgggat
    15361 catcattcat cagcagcttg gttacctcat ttttattgta ggtcaggtta aatcccagag
    15421 ttaaggtcat gtcctgctgc gaaatgggaa taaggtttaa ggcaacttca acacccttat
    15481 tctccatgtt tccaacattg gtcaacagtg tatttttgaa gttacttccg ctggcaatat
    15541 caattgtatt taaaagatcc ttggtttttc tcatgtagag gtcaaccgaa ccactaatac
    15601 ggttattcag aaatccgaaa tcgagtccaa tattctgggt tgttgtttcc tcccacttaa
    15661 tgtcggggtc gtaggcattt ggacgaaggg ttggtaggaa tgttccatca attggatacc
    15721 atgaacctat tgcagatgca acgtattgtg cctggtaagg gtagtcgcta ccaatatcct
    15781 gctgaccggt tactccccag ctggctctaa gtttaaggtc agaaacaaag tttacatttt
    15841 taaggaatga ttcctccttt accttccatg ccaaagctgc tgaagggaaa agcccccacc
    15901 ggttatcttc cgaaaatctt gatgaaccgt catcgcgaag tgtagcagtt atgatgtacc
    15961 tatccattaa ggtatagttt aacctgccaa aaaatgatac aaggtagttt tcggtgataa
    16021 atgaggagct atcggacatt tggtaggggt gatctacatc tactacgccc ctggtgtagc
    16081 tgtcgccctc cctttcaaaa tgctgccagg agtaacctgc catagcttca accttgctct
    16141 ttatttgggt aaggtctttt gtatagttga agtaaaagtc caacaggttg ttcttgtttg
    16201 tagcgctgta atcattcaac tttccccagg ctaagggggc aattagtacc gaaggagaag
    16261 taacgggtct gttgttgtgc ccatcgctgt tggtgtagtc ggtagccatg ttcaggttgg
    16321 cctgtagctc ctgcaggaag gggaacttgt agtttagctg gatgttccca atgaacctgt
    16381 ttacatctgc tttattatcg gcagccatca gctgttccac cgggtttgga gtacccaggt
    16441 tggctccgta gttagcccac tgaaagtatc cggctgaagc agggttgcca tcgtaaatgg
    16501 gttgtgtagg atccatgttg atggctgatc ccagtgctcc ataatcgcca taattctgtt
    16561 tggtagtcat gcccttggca tttaccgtaa cattgaggga gttattcaga aacgatggat
    16621 ttaagctaat tgcaccggtt aacctttcca tgttggtatt cttcataata ccatcctgat
    16681 tggtgtaacc aactgaaacc tggtagggta gaattttata tgctccgctg acgctaacgt
    16741 tgtgatcctg tgaaaaagca ttgcggaaaa tttcgttttg ccagttggtg ttcttagaac
    16801 ctagaagggt taatgtagag gggttgtaaa gtgtgcccat gtggtcgtag gcaatttgcc
    16861 tcatctggtc gcccgagtaa acatccatca gcttaattgc cgaggcaatt gaggtagtaa
    16921 cgttgtagtt tactctaaag gatgctcctt ccttactctt cttggtagta attaaaatta
    16981 cgccgttaga tgctcttgaa ccgtaaatgg cagttgagga ggcatctttc agaatggtga
    17041 ttgtttcaat gtcgttagga ttgacaaacg aaagagggtt tgagctaccc gatacgttgt
    17101 tgttgtcaag tggaacacca tcaacgataa caagtggact gttggatgcg taaagtgacg
    17161 aacccccacg aaccttgatt gtagaaccag atcctggagc tccgttgctt gtggtaatta
    17221 caacaccagg tgttttccct acaagtagct cctgtggaga gctaataacc cccttgttga
    17281 agtcttttga gctaacagtt gttaccgaac cggttagatc agctttcttg gttgttccat
    17341 aaccgattac aacaacctct tctagttgtt cggaggaggt cttcagggct acatccacaa
    17401 cgcttctccc atttaccgga atttcctgtt tctcgtagcc tacgtagcta aataccagaa
    17461 ccgcattcgc ctgggttcta agagagtact tcccatttag gtcagttgca gtaccaatgg
    17521 ttgtaccctt tacaacaacc gcaacgcccg gcaacggttc attgtttgca ttatcggtaa
    17581 ccgttcctgt taccgtaatg tcttgcccat ttgctaggcc ttggtaaaaa acaaggacta
    17641 gaatgaagca aattttcata aaaatgcgtt tgttcttcat ataaaattta atttagggtt
    17701 tggttaaatt ttgcagtgga aagttaagga ttgttaatcc taaaagcatt attttcgttg
    17761 gcttataggc taaaaatcac cgcaaacgat accgcaaacg tttgtatttt tcgttgttta
    17821 tatttaactt tcaccgcaca ggttaaggag gtgttacttt ttatactagg aatatccctt
    17881 tatgaatgta agtatcagga atccaatgta taacatgaag gttgggtgct acactttttc
    17941 cctatccagc aaaacaagga ttagtggctg ttgttttttt taattgataa attagtgctt
    18001 aattagaggg gatattttaa aaacttgttc agtactaacc attactagaa tgtttgctgt
    18061 gggtttttcc taaccacagc cggtgtgtga tgacaaatcg ttactatttg ccagctaata
    18121 atattaaaat tcggaacgga tgaaatcaag ccagattacc atcaaggaca ttgcccgcga
    18181 gctgggcatc agcc
//
LOCUS       DAOSHM010000048        17130 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_2713_length_17130_cov_19.898038, whole genome shotgun
            sequence.
ACCESSION   DAOSHM010000048 DAOSHM010000000
VERSION     DAOSHM010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 17130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17130
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2713_length_17130_cov_19.898038"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            162..305
                     /locus_tag="PK839_07905"
     CDS             162..305
                     /locus_tag="PK839_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72694.1"
                     /translation="MRKPLILLFVVVTLSLASCYKNNATFTVQVNDYAPTYTVAYTAQ
                     NGV"
     gene            838..1923
                     /gene="carA"
                     /locus_tag="PK839_07910"
     CDS             838..1923
                     /gene="carA"
                     /locus_tag="PK839_07910"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HOG72695.1"
                     /translation="MNKQKVKLVLADGSEYSGYSFGYFGSTAGEAVFNTAMNGYPESL
                     TDPSYRGQVLIATFPLVGNYGVPEGITEGNLHRFWESDRVQISGLVVSVYNEDFSHWN
                     AAKSLGDWLAEYRIPAIAGIDTRALTRKIREKGSMLAKILALNDVEWHDPNSENLVAQ
                     VTVAQKEIYGNGEYRVVLVDCGVKNNIIRCLTQRNTTVVRVPYDYDYTDEDYDGLFIS
                     NGPGNPKMNTATIAVLQKAMKSNRPIMGICLGNQLLALASGADTYKLPFGHRSHNQPV
                     ILKGTNRCFITSQNHGFAIDSSTLSDDWDVLFENANDGTCEGIRHKSKPIFAVQFHPE
                     ASGGPNDTEFLFDEFLQLMVKHKQINK"
     gene            1928..5161
                     /gene="carB"
                     /locus_tag="PK839_07915"
     CDS             1928..5161
                     /gene="carB"
                     /locus_tag="PK839_07915"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) large subunit"
                     /protein_id="HOG72696.1"
                     /translation="MNSKYHKVLVLGSGALKIGEAGEFDYSGSQALKALREEGVKSIL
                     INPNIATVQTSWGVADRVYFLPVTPYFVERVVEKERPDGIFLSFGGQTALNCGIELFK
                     TGFLAKHNVQVLGTPVETIINTEDRELFARQLSQINVSTPRSQSARCLDDALQAAEKI
                     GFPLIVRSAFTLGGQGSGFCSNAEELRELSINAFSYSPQILVEESLWGWKEVEYEVVR
                     DRFDNCITVCNMENLDPLGIHTGESIVVAPSQTLTNAEYHKLRSLSIKIIRHLGIIGE
                     CNVQFALDPKSEDYRVIEVNARLSRSSALASKATGYPLAFIAAKLGLGYGLHQLKNPV
                     TAITTACFEPAMDYIVVKIPRWDLNKFSGVSHEIGSSMKSVGEIMAIGRSFEEAIQKG
                     LRMVGQGMHGLVANTKLNFPNLEEALVHPTDMRIFAIAEALELGWTVEKVHELTNIDR
                     WFLGRMQGIITLEKKLMQVASVEMLDRETLLEVKQFGFSDFQLARLIYNPGPDDIETR
                     QQVVRYHRIQLGIVPVAKQIDTLAAEYPSETDYLYLTYHGIENDTSPNQSNRTIVVLG
                     SGAYRIGSSVEFDWCTVSAAGKIRKLGYRAVMINFNPETVSTDYDMCDKLYFDELTFE
                     RVMDIIELEKPIGVMVSMGGQIPNNLAMRLHNAGVKVLGTSPESIDRAEDRQKFSEML
                     DMLHIDQPRWKQLSTLEEVERFVDEVGFPVLIRPSYVLSGSAMNVVSNADELRHYLVL
                     AANVSKTHPVVVSEFIEGAKEIEFDAVANNGEVMFDAISEHIEFAGVHSGDATLVFPP
                     QKLYFETIRRIRKIARAIAKELMITGPFNMQLLALNNEVKVIECNLRASRSFPFVSKV
                     LGFNFVEAATEYMLGRTPGYLPPSMFELDHVGIKASQFSFSRLSKADPVLGVEMASTG
                     EVGCLGTDFDDALLKSMLSVGYRVPQKSILVSSGPLRSKLALVEPLRLIERCGITIYA
                     TRGTAKFLAENDVNTIPVAWPDEDSQPSSIELIGSKQVELVINIPKNLSREELNNDYD
                     IRRATVDYNIPLITNARLAAAFLMAICNKNINTLDLRSWDEFFETKQ"
     gene            complement(5215..6090)
                     /locus_tag="PK839_07920"
     CDS             complement(5215..6090)
                     /locus_tag="PK839_07920"
                     /inference="COORDINATES: protein motif:HMM:NF016103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HOG72697.1"
                     /translation="MRKVIFCIIVCLTAGFSFAQDKNAYTIFDSKGNVASYSDLVNRS
                     AATDVVFFGELHDNPIAHWLELELAKDLFRAKNGSLIMGAEMFEADNQLILDEYMSGL
                     ISMKRFEDEAKLWNNYQTDYKPLVEFAKESGVRFVATNIPRRYASAVAGGGFEVLNSF
                     PDEARKFFAPLPVAFDPELPGYKAMMGMDNMHGAGMQASQNLPKSQALKDATMAHFIM
                     ANFKEGSTFIHYNGSYHSDNKEGIVWYINRTNPNLRVVTVATVMQTDVDKLDAANLGI
                     ADFIIVVPESMTRTY"
     gene            6185..7993
                     /locus_tag="PK839_07925"
     CDS             6185..7993
                     /locus_tag="PK839_07925"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HOG72698.1"
                     /translation="MEITRTFDILEAGKETYENRPVLASRQNGTWEEYSIDKYSQIAH
                     NLSYGLMALGLRPGDRVATVSGNRPEWNFVDMALSQAGMVHVPIYPTISLDEYRYILK
                     HSGVKMIFAGDKSIYGKLQPLTGSLENFIGMYCFQNNNELPSWKEIAEKGKSVANSYI
                     DELFRIKKSISADDVVSLIYTSGTTGISKGVMLTHNNFLSNVRACTGLFPLEPGDKAV
                     SFLPLSHVFERMVNYYYQSIGLTVYYIDNLANLGNVISEVKPQIFMTVPRMLEQTYNK
                     IIAVGKDLSGLKKQVFFWAVNLGYHYNENGNSQYYNMKLRLARKLVFEKWQEAMGGKI
                     KLIVSGGAPLQPRLMRIFWAAGMPVYEGYGLTETSPVIAVNHSATPGNVRFGSVGPVL
                     SNVEVKISNDGEILMRGPSLMKGYYNAPELTSQAIDSDGWFHTGDIGHLDEGKFLTIT
                     DRKKEIFKLSNGKYVAPQLIENKLKESFFIEQCMVVGENEKFVSVLISPNFSFLHDWC
                     SHHKIHFQNNQELITLPEVISRFQKEINGINKQLSDYEQIKRFRLVPEQWSQETGELS
                     PTLKLRRKLLYDRYEKLLEDIYAHPKLVEIRGTNGD"
     gene            complement(7971..9050)
                     /locus_tag="PK839_07930"
     CDS             complement(7971..9050)
                     /locus_tag="PK839_07930"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOG72699.1"
                     /translation="MFFWVLPGVAVAQARPTSLQKPNGTAIIITGAAAKIAQEAALLQ
                     NLYEKGMLKNVVFISGASSGALNAVMLNAILEGKLTWKDYKSILGQLSNEKVFKPDGN
                     RLPVNTTPLRNTISSIVCDRLGYRILSDLPIPTSISVVNFKIAPFSDRTYRLCNLKIN
                     PESDPSLNLVDVLMASTSIPVIFPPSPIKNVRTIPNVPYYDGGIAADHVPYQALVEYE
                     KFRGVPVEKVIIVSRKRDTLSVAEELKQLGVERLGLVERFNISPDAITNRSFFNRLAA
                     MQKEAPELASKTSVFVPDFEHAYCMFNFNKLQEQYETTYSWGKHHDPIPLSDYLGRAG
                     VKPVAEVKPQGQLFNFRVISRHLFP"
     gene            complement(9283..10059)
                     /locus_tag="PK839_07935"
     CDS             complement(9283..10059)
                     /locus_tag="PK839_07935"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOG72700.1"
                     /translation="MSSTTLVALIIIGLVTGVVTGLTGSSGVMIVVPLVNMLLGIPIH
                     ESIGISLIVNVIAPIAISYTYFKNGNIDVRVGVWIALGSIFGAQIGAFFADQIPETGL
                     GGSFGIFIILMGILICIKGFRGKKLESTKPVLEFKTEKRKNMAILMLGFAIGIISGVF
                     GAGGGGMILLILVFVLNFPIRVAIGTSSLLMAITSFSGALGYFIHGNIRLVPGLVMGS
                     GAALSGVVSARFANRVNEDILSIAVGVVFFMLGTLMILIA"
     gene            complement(10239..11399)
                     /gene="dnaJ"
                     /locus_tag="PK839_07940"
     CDS             complement(10239..11399)
                     /gene="dnaJ"
                     /locus_tag="PK839_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HOG72701.1"
                     /translation="MTTKRDFYEILGVSRNATKDEIKKAYRQMALKYHPDKNPGDKEA
                     EEKFKEAAEAYEVLGDDTKRARYDQFGHSGVGNGGGGGGFTHDWTIDDIFSQFGDIFG
                     GHFGSFGGFGGFGNSRANQRATSRGSDIRVKVKLNYSEIANGVEKKLKVNKYVTCHSC
                     NGSGAASPSDFQTCSTCRGSGYVTRVTNTLLGQMQSTAPCPTCNGEGKTITKKCTECN
                     GEGVVRDNEIITIKIPAGVAEGMQLSVSGKGNAARRGGVPGDLLVVIEEEKHPQLIRD
                     GNDLIYNLTISIPDAVLGAPAEIPTVDGKVKIKIEPGTQPGKILRLKGKGLPEVNSYG
                     RGDLLVAINVYIPKTLTREERALFEKLAKSSNFTPNPKESSPIFDRIRGYFD"
     gene            complement(11413..12033)
                     /locus_tag="PK839_07945"
     CDS             complement(11413..12033)
                     /locus_tag="PK839_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HOG72702.1"
                     /translation="MSKSSKQSKAAEDEKELETQQQVDIPEAEDKISAEGAEPNAAPA
                     SENVMEEADATDKALVEKLQKELEELKDKHLRLSAEFDNYRKRTLKEKSDMLKYSAEV
                     VLKDLLPVVDDLERALQAIESTDEITAVKEGLSLIVSKFNDFLNSKGIKEIEAMGSEL
                     NTDLHEAVAKTPVQDESQKGRVVDVIQKGYMLHDKVMRFSKVVVGE"
     gene            complement(12400..12804)
                     /locus_tag="PK839_07950"
     CDS             complement(12400..12804)
                     /locus_tag="PK839_07950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72703.1"
                     /translation="MQKFIRETGVLIAILVLCGSSCDELDSTNTITGVWRSREIYAGN
                     SYRTYNVSMEHYDQPDSTSYIIYNLYNLGLEVETYVQLNDTIFNILGCNSDSYIISGK
                     GYFHPLSFTIDWEYSVSGIDNDPFVMAHFEKP"
     gene            complement(12840..14855)
                     /locus_tag="PK839_07955"
     CDS             complement(12840..14855)
                     /locus_tag="PK839_07955"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018696073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HOG72704.1"
                     /translation="MTREEFQKEILQGIPDELPALQPWDPDVNHAPKRKKILTLEEKK
                     LAVKNALRYFPPKHHAVLAAEFAKELEEYGRIYMYRYRPGYKIFARTINDFPHKSLQA
                     ASIMLMLSNNLDTAVAQHPHELIIYGGNGAIFQNWAQYRLTMKYLAEMTDHQTLVMYS
                     GHPMGLFPSFPDAPRVVITNGMMVPNYSSKDDWERFNALGVTQYGQMTAGSYMYIGPQ
                     GIVHGTTITVINAGRMHSKPGEKALGGKVFVSSGLGGMSGAQPKAAVIAGVVGVIAEV
                     NPKAVEKRYQQGWVNEVYTDLEKLIPRIREASARKEAVSLAYQGNVVDLWERLAREDL
                     RVDLGSDQTSLHNPFAGGYYPAGLSFEESNRMMVDNPELFRQKVYESLRRQVKAISAL
                     TERGMYFFDYGNAFLLESSRAGADVLKPNGEFKYPSYVQDIMGPLYFDYGFGPFRWVC
                     TSSNPSDLAETDAIALEVLENIASHAPEEIQQQLHDNIHWIREAGRNNLVVGSQARIL
                     YADSEGRLRIAEAFNKAIREGRISAPVVLGRDHHDVSGTDSPYRETSNIYDGSRYTAD
                     MAIQNVIGDSFRGATWVSIHNGGGVGWGEAINGGFGLTIDGSADAERRLKMMLHWDVN
                     NGIARRSWARNHGAISSIKREMERTPLLKVTLPNMADDNLIDDVVKG"
     gene            15026..15628
                     /locus_tag="PK839_07960"
     CDS             15026..15628
                     /locus_tag="PK839_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOG72705.1"
                     /translation="MKSKTWITIGIVVLVAVILYISIKGSYNKMVQKDEAVTSQWANV
                     ENVYQRRLDLIPNLVNTVKGYANFEQQTLTQVVEARAHATQVKVSPENLNQESIQQFQ
                     AAQNELSGALSRLMVVVEQYPELKANQSFLDLQAQLEGTENRIAVERNKFNEVVRDYN
                     TYIRRFPSNIWAALFNFDKRGYFESEAGAEKAPKVDFGTN"
     gene            15695..16129
                     /locus_tag="PK839_07965"
     CDS             15695..16129
                     /locus_tag="PK839_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HOG72706.1"
                     /translation="MKAKDYFTLEEQKRITDAVEQAEKNTSGEIRVHVENECRIDVLD
                     RAVQIFAMLKMHKTKLRNGVLIYLSLKDHKFAILGDAGINAKVPEDFWDSTKQAMLEE
                     FQENRLTEGIIKGITMAGEKLKEHFPYKQDDVDELTNDISFG"
     gene            16159..16944
                     /locus_tag="PK839_07970"
     CDS             16159..16944
                     /locus_tag="PK839_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HOG72707.1"
                     /translation="MITKQLRNILLAAALLLPNISWSQDIPDPMNPPRLVNDFAGILG
                     GEERDALESMLRGYHDSTSTQIYVVTVNDLSGYDISDYAFRLGEKWGVGQKSKNNGVV
                     ILIKPKVGNQKGQAFIATGYGMEAIIPDAVARRIVDNEMIPYFKTNSYYKGIVAGCKV
                     IIDLAGGEYTADAYYKKESPIPGIILLIIFFGFIFLSGGRRSKLRSGSIGKNIPFWIL
                     LGSMGSGRRGGGFGGFSSGSGGFGGFSGGGGGSFGGGGAGGSW"
BASE COUNT         4722 a         4064 c         3944 g         4400 t
ORIGIN      
        1 gttagtagct attctataag gtgaatgttg ctgtatgttc agtagaatga accgatgtgg
       61 catgccactg cgtaaggact tgatttggtg gttaatattt ttgagaaaat tccaagaaat
      121 ttacctgcta ttatcgtcga ctaactaaat aggttagcgc catgagaaag ccattgatat
      181 tactttttgt ggttgttacc ttgagtttgg catcatgcta caaaaacaac gctacattta
      241 ccgttcaggt aaacgactat gcgccaacct acacggttgc ttacacggct caaaatgggg
      301 tatgattggg cagcctttaa gttccgaaaa actatgagtt tttcaacgag tactcctatg
      361 gctggcagct aaacctcaag gttaaggcgg aagacagcat atttgtatat gtgcaagaac
      421 gagggaacct gaaagaacca attataagtg taaggattca ctagaagttc ataaaatcca
      481 taccattctg aaccaattaa ttcatgtgaa attggatttg aattgcatca ttttcttaga
      541 aaggtttaat tgatccatgt agctgtttac cagtattgta gcaaaatatg agtttgcaat
      601 ttaaggggtg gctatatatt tcaaagattt cttttacttt tactccgtaa aacgcagcta
      661 tatgtttcag gttgatagaa taatactcag cagcaagcac acaggatttg aaaattcaac
      721 atgcttttgt aatgaatgtc tgcccattcc ggcagacttt tttttttacc caacagttgg
      781 cggtagctgc agctgcaaaa accacatcgc aaaataacta aacagctaaa actatctatg
      841 aataagcaaa aagtaaagct ggtgctggca gatgggagcg agtattccgg ctactccttt
      901 gggtatttcg gcagcactgc aggtgaggcc gtattcaaca ccgccatgaa cggttatccc
      961 gaaagtctta ccgatccctc ctaccgtgga caagtactaa ttgccacctt cccgttggtt
     1021 ggaaactacg gcgttccgga aggaataacc gagggaaatc tacataggtt ctgggaatca
     1081 gatagagttc aaatatctgg gctggttgta tcggtatata atgaggattt cagccactgg
     1141 aatgccgcca aaagtcttgg cgattggctt gctgaatacc gaattcctgc aattgccggc
     1201 atcgacacaa gagctttaac ccgtaaaata cgggagaagg gtagcatgct ggctaaaata
     1261 ttggctttaa acgatgtgga atggcacgac ccaaactccg aaaacctggt ggcccaagtc
     1321 acggtagctc aaaaagaaat ctatggaaat ggggagtaca gggtagttct ggtggattgc
     1381 ggggtaaaaa acaacattat ccgatgttta acgcaacgca acacaacggt ggtaagagtt
     1441 ccctacgatt acgattacac cgatgaagat tacgatggct tgttcatatc caacggaccg
     1501 ggaaatccaa aaatgaacac tgccacaatt gcggttctgc aaaaggcaat gaaaagtaat
     1561 aggccaatta tgggtatatg cctggggaat cagctgctgg ctttggcatc cggcgctgac
     1621 acctataaac ttccctttgg acacagaagc cacaaccaac ctgtaattct taagggaaca
     1681 aacaggtgct tcattacctc gcaaaatcat ggctttgcaa ttgactcctc aaccctttcg
     1741 gacgactggg acgtgctttt tgagaatgcc aacgacggaa cttgtgaggg catcaggcac
     1801 aaatctaaac caatatttgc agtgcagttt catcccgaag cgtcgggagg cccaaacgat
     1861 actgaattcc tatttgacga atttcttcag ttgatggtaa agcataaaca aatcaacaag
     1921 taaccgtatg aacagcaaat atcataaagt actggtgctt ggctcgggag cccttaaaat
     1981 cggcgaagcc ggagagtttg actacagcgg atcgcaggca ctaaaagccc tgagggagga
     2041 gggggttaaa agcatactga taaatcctaa tattgccacc gttcaaacca gctggggagt
     2101 tgccgatagg gtttactttc taccggtaac tccgtacttt gtagagcggg tggttgaaaa
     2161 ggagcgccct gacggaatat ttttatcgtt tggaggtcaa acagcattga actgtggaat
     2221 tgagctcttc aaaacaggat ttcttgctaa gcacaacgtt caggtgctag gaacaccagt
     2281 tgaaaccatc atcaacacgg aagacaggga gctgtttgcc agacaactct cacaaattaa
     2341 tgttagcacg ccccgcagcc aatcagcaag atgccttgat gatgcactac aggccgccga
     2401 aaaaattggt tttccgctta tagttcggtc ggcattcaca ttgggaggtc agggaagcgg
     2461 tttctgctca aacgctgaag aactccggga gctttccatt aatgcctttt catactcccc
     2521 tcagattctt gttgaggaat cgctatgggg atggaaggag gtggagtatg aggtagttcg
     2581 cgaccgcttc gacaactgca tcaccgtttg caacatggag aacctcgacc ccctgggtat
     2641 ccatactggt gaaagtattg tagttgcacc ctcgcaaacc cttaccaacg ctgaatacca
     2701 caagctacgc tcactttcca ttaaaatcat aagacatctg ggcattattg gggagtgcaa
     2761 cgttcagttt gccctcgatc cgaaaagtga ggactaccgt gtaattgagg taaacgcccg
     2821 tctgtcgcgc tcgtcagcac ttgcctcaaa ggcaacaggc tacccacttg cctttattgc
     2881 tgcaaaactc gggttgggct acggccttca tcagcttaaa aaccctgtaa cagccattac
     2941 cactgcctgt tttgagcctg caatggatta catcgtcgta aaaattcccc gatgggattt
     3001 gaataagttt tcgggcgtca gccacgaaat aggctcatcc atgaaaagtg taggagagat
     3061 aatggccatt ggccgctcct ttgaggaggc cattcagaag ggtctcagga tggttggaca
     3121 gggcatgcac gggctggtgg ccaacacaaa gctcaacttt cccaacctgg aagaggccct
     3181 ggtccaccct accgatatgc gaatttttgc cattgccgaa gcattggagc tggggtggac
     3241 ggttgaaaaa gttcacgagc tgacaaatat tgacaggtgg ttcctcggaa gaatgcaggg
     3301 aatcattacc cttgagaaaa agctgatgca ggttgcatca gttgaaatgc tggataggga
     3361 aaccctgctt gaggtgaagc agtttggctt ttccgacttt cagctggcca ggctgatata
     3421 caatcctggt ccagatgata tcgaaacccg ccagcaagtg gtacgatacc acaggataca
     3481 actcggtatt gttcctgtgg ccaagcaaat agacacgctg gcagccgaat atccttccga
     3541 aacggattac ctctacctga cctatcatgg tattgaaaac gacacatcac ctaaccaaag
     3601 caaccgaacc atagttgtac ttggttcggg agcctaccgg attggcagct ctgttgagtt
     3661 tgactggtgc acggtaagtg cagccggcaa aataaggaag cttggctaca gagcagtaat
     3721 gatcaacttc aatcccgaaa cggttagcac cgactacgac atgtgcgaca agctgtactt
     3781 tgatgagcta acgtttgaac gggttatgga catcattgag ttagaaaaac ctattggtgt
     3841 aatggtctcc atgggaggcc aaatccccaa taacctggcc atgaggcttc ataatgcagg
     3901 agtaaaagta ttgggaactt cgcccgaatc catagatagg gctgaagatc ggcaaaagtt
     3961 ctccgaaatg ctcgacatgc tgcacatcga ccagccccga tggaagcagc tatccaccct
     4021 cgaggaggtt gaacgttttg ttgacgaggt gggcttcccg gttctcatac gtccatccta
     4081 cgtgctgtcg ggctcggcga tgaatgtagt ttccaacgcc gatgagcttc gtcactacct
     4141 cgttctggcg gctaatgtat ccaaaaccca cccggttgtc gtaagcgaat tcatcgaggg
     4201 tgccaaggaa atagaattcg atgccgtggc caacaacggg gaggtgatgt tcgacgccat
     4261 ttcggagcat attgaatttg caggtgtgca ctccggtgac gcaaccctgg tttttccacc
     4321 gcagaagcta tactttgaaa ccatccgtcg aatccgaaaa attgccaggg caatagctaa
     4381 ggagctcatg attacaggcc cattcaacat gcagctgctt gcactgaaca acgaggtcaa
     4441 ggtgattgag tgcaacctga gagcaagcag gagcttccca tttgtctcaa aagttttggg
     4501 cttcaacttt gttgaggctg caacggagta catgctgggt cggacacccg gctacctacc
     4561 tccttccatg tttgaacttg accatgtggg cataaaagcg tcgcagttct catttagccg
     4621 tctttcaaag gccgatccgg tactgggcgt tgagatggcc tcaaccggag aggttggctg
     4681 tttgggaacc gattttgacg atgcccttct gaaatcgatg ctttcggtgg gctaccgagt
     4741 accgcaaaaa tccattctcg tttcgtcggg accattacgg tcgaagctgg ccttagtaga
     4801 acctttacga ctaattgagc gttgcggcat tactatctat gccactcgtg gcactgccaa
     4861 attccttgcc gaaaatgacg taaacactat tccggtggca tggcccgatg aggacagcca
     4921 gcctagcagt atcgagctaa ttggtagtaa acaggttgag ctggtgatca acatacctaa
     4981 aaacctatca cgagaggagc tgaacaacga ttacgacata aggcgcgcaa cggttgacta
     5041 caacattccg cttattacca acgcccggtt ggcagcagca ttcctaatgg ccatctgcaa
     5101 taaaaacata aacactctcg acctaagaag ctgggatgaa ttttttgaaa ccaaacaata
     5161 ataaatgcat gtatttctga acgttaatta tattttcctg cgtttaagca ccgactagta
     5221 ggttcgggtc atgctttcgg gaaccactat gatgaaatcg gcaatgccca aatttgcagc
     5281 atccaacttg tcaacatcgg tctgcatcac agtagccacg gttaccaccc ttaggttggg
     5341 gttggtccgg ttgatatacc agactatacc ttccttgtta tcggaatgat aggaaccatt
     5401 gtagtggata aaggtggaac cttctttaaa gttggccatt atgaaatgag ccatggtggc
     5461 atcctttaat gcctgcgatt tgggcaagtt ctgagaggcc tgcatgccag ctccgtgcat
     5521 gttgtccata cccatcatag ccttatagcc cggaagttca ggatcgaagg caaccggtaa
     5581 gggtgcaaaa aattttcggg cttcatcagg aaaactattc agcacctcaa agcctcctcc
     5641 agccactgca gaggcgtacc tgcggggaat attggtggca acgaacctaa ccccactttc
     5701 cttggcaaac tcaaccaacg gtttatagtc ggtctggtag ttgttccaaa gttttgcctc
     5761 atcctcaaag cgtttcatgg agataaggcc agacatgtac tcgtcgagta tgagctggtt
     5821 atcggcctca aacatctcgg ctcccataat caggctgccg tttttcgccc taaaaaggtc
     5881 ttttgccagc tccagctcca gccagtgggc tatggggtta tcgtggagct ctccaaaaaa
     5941 cacaacatcg gtagctgccg atctattaac taaatcagag tacgaagcca catttccctt
     6001 tgaatcgaaa atggtgtagg catttttatc ctgggcaaag gaaaaccctg ctgttaaaca
     6061 aacgattatg caaaaaatga cttttctcat atttttttat atttgccaaa cctgggattt
     6121 acacctttag ctaaacaacg agctaaagta aacattttta acaatttttt ctaagcatgg
     6181 cgatatggaa atcacacgaa cattcgacat tctggaagcc ggaaaggaaa cctatgagaa
     6241 tcgtccagtt ctggcatcca ggcaaaacgg aacctgggag gagtactcca tagataagta
     6301 ctcacaaatt gcgcacaacc tgagctacgg attgatggcc ctaggtctaa gacctggcga
     6361 tagggtggcc accgtgtcgg gtaaccgacc cgaatggaac ttcgttgaca tggccctttc
     6421 gcaggcaggc atggtgcatg tacccatcta ccccaccatc agccttgatg agtataggta
     6481 catccttaag cattcggggg taaaaatgat ttttgcaggc gataagagta tttacggcaa
     6541 gcttcaaccc ctcaccggta gccttgagaa ttttatcggc atgtactgct ttcagaataa
     6601 taacgaactt cccagctgga aggaaattgc tgaaaaaggg aaaagtgttg ccaacagcta
     6661 tattgatgag cttttcagaa taaaaaaatc aattagtgcc gatgatgttg tttcactaat
     6721 ctacacttcg ggaacaacgg gcatatcaaa aggtgtaatg cttacccata ataattttct
     6781 cagcaatgta agggcttgca ccggactttt tccgttagaa ccaggagata aggccgttag
     6841 ctttcttcca cttagccacg tatttgagcg tatggtgaac tactactacc aaagcattgg
     6901 ccttacagtt tactacatcg acaacctggc caacctgggg aatgtcatca gcgaggtaaa
     6961 gccacagata ttcatgaccg taccgcgaat gcttgagcaa acatacaata aaattatagc
     7021 ggtaggaaag gatctttccg gtctgaagaa acaggttttc ttctgggcgg ttaatctggg
     7081 gtaccactac aacgagaatg gcaatagcca gtactacaac atgaagttga gactggcccg
     7141 taagctggtt tttgagaaat ggcaggaagc gatgggagga aagataaagc tgattgtaag
     7201 cggcggtgca ccgctccaac cccgtttgat gagaattttc tgggcagccg gcatgccggt
     7261 ttacgagggc tacgggctaa cggaaacctc accagtgatt gccgtcaacc acagcgctac
     7321 ccccgggaat gttcgatttg ggtcggttgg ccctgtcctt tctaatgttg aggtgaaaat
     7381 atccaacgat ggagaaatac tcatgcgcgg ccccagcctg atgaagggct actataacgc
     7441 tcctgagctt acaagccaag caattgactc cgatggatgg tttcataccg gtgacattgg
     7501 acaccttgat gagggaaaat tcctaaccat taccgacagg aaaaaagaaa tctttaagct
     7561 ttccaacggc aagtatgttg cccctcagct gattgaaaac aagctgaaag aatcattttt
     7621 cattgagcag tgcatggtag ttggtgaaaa cgagaagttt gtcagcgttc tgatttcacc
     7681 caacttcagc tttctccacg attggtgcag ccaccacaag atacacttcc agaacaacca
     7741 ggaacttatc accctacccg aggtgatttc ccgatttcaa aaagaaataa atggcataaa
     7801 caagcagctg agcgactacg agcagatcaa acgctttagg ctggttccgg agcaatggtc
     7861 gcaagaaaca ggcgaacttt caccaacgct caaactccgt cggaagttgc tgtatgaccg
     7921 gtatgaaaaa ctattggagg acatttacgc tcatccaaag ttggttgaaa ttaggggaac
     7981 aaatggcgac taattactcg gaaattaaat agttgaccct ggggttttac ctctgccacg
     8041 ggtttaacgc cagccctccc caaataatcg ctcaagggaa tcggatcgtg atgtttaccc
     8101 cagctgtagg ttgtttcata ctgctcctgc agcttgttaa agttaaacat acagtaggca
     8161 tgctcaaagt caggtacaaa tacgctggtt ttactagcca actccggagc ctccttttgc
     8221 attgcagcca accggttgaa gaaacttctg tttgttatcg catcgggcga tatgttgaac
     8281 ctttccacca gacctaacct ttcaacgccc agctgcttga gttcttcagc aactgaaagg
     8341 gtatcgcgct tacgactcac gataatcact ttttcgacgg gcacaccccg aaatttctca
     8401 tactcaacta gtgcttggta tggcacatgg tcggcggcaa ttccgccgtc gtagtaggga
     8461 acattaggta tggttcttac atttttaatg ggggacggtg gaaaaattac cgggattgag
     8521 gttgatgcca tcagaacatc aactaagttg agcgaaggat cgctttcagg gttaattttt
     8581 aggttgcaaa gccggtatgt tctatcgctg aaaggggcta ttttgaagtt aaccaccgaa
     8641 atggaagtgg gaatcggcaa atcggaaaga attcggtagc ctaacctatc gcacacaatg
     8701 ctgctaatag tatttcttag cggggttgta tttacgggta acctgttccc atccggttta
     8761 aaaaccttct cgttcgatag ctgacccaga atgctcttgt aatccttcca cgttaactta
     8821 ccctcaagaa ttgcgttcag cataacagca ttaagcgccc cggagctggc gccagaaata
     8881 aataccacgt ttttaagcat tcccttttcg tagagattct gaagaagggc tgcctcctgg
     8941 gctatttttg cagcagctcc tgttattata attgcagtgc cgttgggctt ttgaaggctg
     9001 gttggcctgg cttgggcaac tgctacacct ggcaataccc aaaaaaacat cagtgctaca
     9061 atgcaatacc tttttactga aaagaaagcg ggttttaacc agcacagcca taaattcgtc
     9121 ataatcttcg tatgaaatta gctcatgtcc aaacgctgct atatagcttt ggttgtctgg
     9181 cttaaggctt aaggatactt gccgtcattt tacttcaaaa taggcttagt ccaatgttaa
     9241 agcatccccc cacaacggct gctgccacag caaagagtca aatcatgcta tcagtatcat
     9301 taaagtcccc agcataaaaa ataccacccc aacagcaatg gaaagaatat cctcgttaac
     9361 cctattggca aacctggcgc tgactacgcc gctcaaggca gctcccgacc ccatcacaag
     9421 tccaggaaca agccttatat tgccatgaat aaagtaacca agtgctcccg agaacgaagt
     9481 tatagccata agaagcgacg atgttcctat agctaccctt atgggaaagt taagcacaaa
     9541 aactaaaatc aaaagaatca ttcctcctcc cccggcaccg aaaactccag aaataatgcc
     9601 tatggcaaaa cccagcataa gaattgccat attcttacgc ttctccgttt tgaactccaa
     9661 aaccggtttg gtactttcca gctttttccc tctaaaaccc ttaatgcaaa tcaggatgcc
     9721 cattagaata atgaatattc caaaagagcc ccccagtccg gtctccggaa tttgatcggc
     9781 aaaaaatgct ccgatttgag ccccaaaaat tgaacccaac gcaatccaaa cgcctaccct
     9841 tacatcaata ttgccgtttt taaagtaggt ataagaaata gctatggggg ctataacgtt
     9901 gactatcagg ctaattccta tagattcatg aataggaata cccagcagca tgttaaccag
     9961 aggaacaaca atcataaccc ccgacgatcc ggtgagacct gttactactc cggttacgag
    10021 tccaatgatt attaaagcta ccagtgtagt tgacgacatt acaacattat gtaattatag
    10081 tacgtatcca gaggctaagt taaagcagaa agccgagtaa gcaaaaatca acatagcttc
    10141 aacgtaattg acaaaccagc gctccttaac ttcaaaaata ccatcaccaa cagcagctgt
    10201 tcatttattt tatattgaaa ttgtaaagtc tgtaaaagct aatcgaagta gccgcgaata
    10261 cggtcaaata tgggagacga ctcctttgga ttgggtgtaa aattggatga cttggcgagt
    10321 ttttcgaaaa gggctcgctc ctcacgggtc aaggtctttg ggatgtaaac gttaatcgcc
    10381 acaagtaaat cgccacggcc atagctgttt acctcgggaa gtcctttacc cttcaaccta
    10441 aggattttac caggttgagt tccgggttca atcttaattt tgaccttccc atccaccgtt
    10501 ggaatttcgg caggagcgcc cagaaccgca tcgggaatgc tgatggtaag gttgtagatc
    10561 aggtcgttgc catcgcggat tagctgtggg tgtttctcct cctcaataac cacgagcaag
    10621 tcgccgggca cgccacctcg tcgggcagcg ttacccttac ctgacacgct gagctgcata
    10681 ccctcggcta caccagcggg aattttaatg gtaataatct cgttatccct caccacgcct
    10741 tcgccattac actccgtgca ttttttagtg atggttttac cctcaccgtt gcaggtggga
    10801 catggtgcgg tgctttgcat ctgccccaac agtgtgttgg taactcgtgt aacatatcct
    10861 gatcccctgc aggtggagca tgtctgaaag tccgatggac tggcagctcc gctaccattg
    10921 cacgaatgac aggtaacgta cttgtttacc tttaactttt tctctacacc attggctatc
    10981 tcgctgtagt tgagcttaac cttaacccgt atatcgcttc cacgcgaggt tgccctctgg
    11041 ttagccctac tgttgccaaa tccaccaaat ccgccaaaac tgccaaaatg accgccaaaa
    11101 atatcaccaa actgtgaaaa aatatcatca atggtccagt catgggtgaa accgccacct
    11161 ccaccaccat tgcctactcc ggaatgccca aactggtcgt agcgagcacg cttggtgtca
    11221 tcgcctagca cttcgtaggc ctcggccgcc tccttaaact tttcctcggc ttccttatca
    11281 cccggattct tgtcggggtg atacttcagc gccatttgcc ggtaggcctt tttaatttca
    11341 tcctttgttg cattccggct aacgccaaga atttcgtaaa aatctctttt tgtcgtcatg
    11401 ctctacccac tcctattcgc caacaacaac ttttgaaaaa cgcatcacct tgtcgtgaag
    11461 catatatccc ttctgaatga catctactac tctccccttc tgtgattcat cctgaacggg
    11521 agttttggca accgcctcgt gcaaatcggt gttcagctcc gagcccattg cttcaatttc
    11581 cttaatgccc ttagaattga gaaaatcgtt aaattttgaa actatcagtg ataggccttc
    11641 cttgactgca gtaatctcat cggttgactc aatagcttga agggctctct caaggtcatc
    11701 aaccaccgga agcaaatcct tcagtacaac ctcggcagag tacttgagca tatccgattt
    11761 ctccttcagc gttcttttac ggtagttgtc aaactcggcc gaaagtcgca ggtgcttgtc
    11821 cttaagctcc tccaactcct tctgtaactt ctcaactaag gctttatcgg ttgcatcagc
    11881 ctcttccatt acgttttccg aagcaggtgc tgcattgggc tcagcccctt cggcggaaat
    11941 tttatcctca gcctcaggaa tatctacctg ctgctgggtt tccagctcct tttcatcctc
    12001 agcagctttg ctttgcttgc tactcttact catatctcaa ttatatttgt ttcattttta
    12061 gatggttaca cgataaaccg cttacctaca aataatgaag taatacttca taggggaaaa
    12121 tccttgttat acacaattta aataccatgc agaaacgctc ccccaccgcc attctgtcgc
    12181 ccagtccaat caaatattat tccagcagtg caatggcaga caaaatgaca caaaagtagt
    12241 aaaaaatagg ggatgtttat accgatagcc aacgcttaat tgttcggctt ttatcagaat
    12301 aaagaaattt tagcagttaa actactgctt gttacatcag tattctcgag cacagatatt
    12361 attatgggga caaaaaagaa taagtgataa acccctggtc tagggttttt caaaatgtgc
    12421 catcacgaag ggatcgttgt caatgcccga aacgctgtac tcccagtcaa tggtaaagga
    12481 aaggggatga aagtacccct ttcctgaaat gatgtaggaa tcgctgttgc agcccagtat
    12541 gttgaaaatc gtatcgttaa gctgaacata ggtctccacc tcaaggccca ggttatatag
    12601 gttataaatt atgtaggagg ttgagtcggg ctggtcgtag tgctccatgc taacgttgta
    12661 tgttctgtag ctatttccgg cgtatatttc cctactccgc cacacgccgg taatggtgtt
    12721 ggtgctatcg agctcgtcgc agctactgcc acataggacc aagatggcaa tcaacacgcc
    12781 tgtttcccga ataaatttct gcatagccat tgcatcaagg tgaaagggtg aaggagcgct
    12841 taccccttca ccacgtcatc aataaggttg tcatcagcca tgttgggaag tgttaccttt
    12901 aacaggggtg tgcgttccat ttcccgtttt atggatgaaa ttgcaccgtg gtttcgagcc
    12961 cagcttctac gggcaattcc gttgttcaca tcccagtgga gcatcatttt aaggcgtcgt
    13021 tcggcatcgg ctgaaccatc aatggtaagc ccaaaccccc cgttaatggc ctctccccat
    13081 ccaacacctc cgccgttatg gatggaaacc cacgttgccc cgcggaatga atcgccaatc
    13141 acattctgaa tggccatatc ggcagtgtag cgtgatccat cgtaaatgtt ggaggtttcg
    13201 cggtaggggg aatcggttcc cgaaacatcg tggtggtcgc gcccgagcac aaccggggcg
    13261 gaaattctgc cctcccggat agccttattg aaggcttcag caattctaag cctgccctcc
    13321 gaatcggcgt aaagtattct ggcctgcgag ccaacaacca ggttgttcct accagcttcc
    13381 ctaatccagt gtatgttgtc gtggagttgc tgttgaatct cctctggagc atgacttgca
    13441 atattctcca gaacctccag ggctatagca tcggtttcgg caaggtcgga agggttggaa
    13501 gaggtgcaaa cccatcgaaa tgggccaaag ccatagtcga agtacagcgg acccattatg
    13561 tcctgaacat aggagggata tttaaactcg ccattaggtt tcagcacgtc ggcaccagcc
    13621 ctgctactct caagcaggaa ggcgttgccg taatcgaaga agtacattcc acgttccgtc
    13681 agggctgaaa tggccttcac ctggcggcga aggctttcgt aaaccttctg cctaaacagc
    13741 tcaggattgt caaccatcat ccggttcgat tcctcaaagg atagccccgc aggatagtag
    13801 cctccggcaa atgggttatg aagcgaggtt tggtcgctac ccaggtcaac ccgtaggtct
    13861 tctctggcaa gcctttccca caggtcaacc acatttccct ggtaggccaa cgacactgct
    13921 tccttccgtg ccgaggcctc gcgaattcgt ggaattagct tttccaaatc ggtgtaaacc
    13981 tcgttcaccc atccctgttg gtaccgtttt tccactgcct ttgggttgac ctcggcaatc
    14041 acccccacca cgccggctat aacggcagct ttaggctgtg ccccagacat tccgcccaat
    14101 ccggaggata caaatacctt ccctcccaat gctttttcgc cgggtttaga gtgcattcgt
    14161 cctgcattga taaccgttat ggtggtgccg tgaacaatgc cctgagggcc tatatacatg
    14221 taggaacctg cggtcatttg cccatactgc gtaacgccaa gagcgttaaa acgctcccag
    14281 tcgtccttag aggagtagtt gggaaccatc atcccgttgg taattaccac cctgggggca
    14341 tctggaaatg atgggaaaag tcccattgga tgtcctgagt acatcacaag ggtttggtgg
    14401 tcggtcattt cggccaagta cttcatggtt aacctgtact gtgcccaatt ttgaaaaatg
    14461 gcgccattac ctccgtagat gataagctca tgggggtgtt gggcaacagc tgtgtcgaga
    14521 ttattgctca gcatcaacat tatagaggct gcctgcagcg atttgtgggg aaaatcgttt
    14581 attgtccggg cgaaaatttt atagccagga cggtaacggt acatgtaaat tcttccatac
    14641 tcctcgagct ctttggcaaa ttcggcagct aaaacagcat ggtgtttcgg gggaaagtag
    14701 cgaagagcgt tcttaacagc cagcttcttc tcttcaaggg taagaatttt ctttcgtttg
    14761 ggcgcatggt taacgtccgg gtcccatggc tgaagggcgg gtaactcgtc ggggatgccc
    14821 tgaagtatct ccttttgaaa ttcttcgcgt gtcatacttt ttgaatgttt ttaacctagt
    14881 gttttgccac aaatttaaga ttttaaaccg actttgaacg gcttacccga aattgtaaat
    14941 tatattaatt taaccggtat gcattttttc atttgataaa taaagctact tttggcaagt
    15001 ttaaaacata tcaaattaaa gaactatgaa gagtaaaaca tggataacca ttggaatagt
    15061 agtgttagtg gctgttatac tatatatttc cattaaagga tcgtacaaca aaatggttca
    15121 aaaggacgag gcagttacct cgcaatgggc caacgttgaa aatgtatacc agcgcaggtt
    15181 agaccttata cccaacctgg tgaatacggt gaagggctat gccaactttg agcagcaaac
    15241 cctgacccag gtagtagagg ccagagcaca tgccacacag gtaaaggttt caccggagaa
    15301 tctgaatcag gagagtatcc agcaattcca agccgctcag aacgagctga gtggggcgct
    15361 ttcgcgctta atggtggtag tagagcagta ccccgagctt aaggccaacc agagctttct
    15421 cgaccttcag gcacagctgg agggaacaga gaatcgtata gctgttgagc gcaataagtt
    15481 caacgaagtg gttagggact ataacaccta cataaggcga ttccccagta atatttgggc
    15541 agcactattc aacttcgata agaggggata ctttgaatcc gaggccggag ccgaaaaggc
    15601 gcccaaagtt gattttggca caaactagcc aaatatatag agatagctgg ccacaacttt
    15661 acgctggcac aaccatgccc atcaatccaa aaaaatgaag gcaaaggatt actttactct
    15721 cgaggagcaa aaaagaatta cagacgcggt tgagcaagcc gaaaaaaaca cttcaggcga
    15781 aatcagggtg cacgtggaga acgaatgccg aattgatgtg ctcgacaggg ccgttcaaat
    15841 ttttgccatg ctaaaaatgc acaaaacaaa gctgagaaac ggcgtgctga tctacctctc
    15901 cctaaaggac cacaagtttg ccatactggg cgatgccgga ataaatgcaa aggttcctga
    15961 agatttttgg gattcaacca agcaggcgat gcttgaagag tttcaggaaa acaggctgac
    16021 tgaagggata attaagggaa taaccatggc tggcgaaaag ctcaaggagc atttccccta
    16081 caagcaagac gatgtagatg aactcaccaa cgatatttcg tttggctaaa cccattcttc
    16141 acattaagca acccaaccat gatcaccaaa caactcagga atattcttct agcggcggcg
    16201 ctattgcttc caaatatttc gtggagccag gatattcccg accccatgaa tccaccccgg
    16261 ctggttaatg attttgccgg gattctcgga ggcgaagagc gggatgcact cgaatcaatg
    16321 ctgcgtggct accacgactc cacctcaacc cagatatatg ttgtaacagt aaacgatcta
    16381 tctggctacg acatatccga ctatgctttc cgcctgggcg aaaaatgggg agttggccaa
    16441 aaaagcaaaa ataatggcgt ggttatcctg attaagccta aggtagggaa tcagaaaggg
    16501 caagctttca tagccaccgg ttacggaatg gaggctataa ttcccgacgc cgttgctcgc
    16561 agaattgtgg acaacgagat gattccctac ttcaaaacga acagctacta caaggggatt
    16621 gtggcgggct gcaaggtaat aatcgacctg gccggaggag agtacactgc tgatgcttac
    16681 tacaaaaagg aatcgcctat tccgggaatt atcctcctga tcatattttt tggattcata
    16741 ttcctttcgg gcggcaggcg gtcaaagcta cgttcaggat ctattgggaa aaacattccg
    16801 ttctggatcc tactaggctc aatgggcagc ggaagacgtg gtggtggatt tgggggattt
    16861 tcttccggct cgggcggatt tggaggattt agcggaggag gcgggggtag cttcggcggt
    16921 gggggtgccg gaggaagctg gtaacctgcc gatccttatt ctttgcagct aatcatctca
    16981 accgtacatt ctactctgta ctctccgtgg ttatgtcttt ctcaccacag agagcacgaa
    17041 cagcacagag agagttaaag tttctgctct gtgtacctct gtgtcctccg tggttatgtc
    17101 tttcgcacca cagagagcac ggagcagcac
//
LOCUS       DAOSHM010000049        16795 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_2784_length_16795_cov_9.573716, whole genome shotgun sequence.
ACCESSION   DAOSHM010000049 DAOSHM010000000
VERSION     DAOSHM010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 16795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16795
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2784_length_16795_cov_9.573716"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            111..845
                     /locus_tag="PK839_07975"
     CDS             111..845
                     /locus_tag="PK839_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOG72708.1"
                     /translation="MNYTIDHSSSIPLHVQVENLLREMIRDPEFANGKFLPNEVDLAK
                     RLGISRNTLRQATNKLTYEGLLVRKKGVGTIVATKSVNTRLMNWPSFSQEMKALGIKI
                     INYELHVSWEQADKKIADFFRISEGKKVLKLERLRGGPDGPFVYFISYFHPRVGLTGN
                     EDFSKPLYEILEKNYATIAQLSKEEIIAIAANEFLAQKLGMKPGDPILKRKRYVFDPG
                     SRPIEYNLGFYRGDSFVYTIESERKL"
     gene            1035..1922
                     /locus_tag="PK839_07980"
     CDS             1035..1922
                     /locus_tag="PK839_07980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72709.1"
                     /translation="MKRKMNFPLLLVCITLFCIPFAISSCNDEDEVKTNATISTSSTP
                     VLAVGESATATINIVAEGIKSFKYYKVVDEVRAVEYDAINNLVKEGNKYTYSFSYTVQ
                     EFDDLHTLGFEFEIVDKANVSYVLGLVVNTELSLKSSFVKFDWKVTASEWLGMDVLAP
                     HDAAYIYRFHEDGTYEVDMTPEYADNLHHFCYWVFKETPDNGDTLAVIRLVRKLRSGE
                     TAVDEYYDYRIVNATQAEMTMYWDIPVFGMFNIANTFTSQPKGAFQPYGTPEMASEVG
                     NDPVMSCSTVDPALLTIQK"
     gene            2332..5415
                     /locus_tag="PK839_07985"
     CDS             2332..5415
                     /locus_tag="PK839_07985"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOG72710.1"
                     /translation="MKKILISTFFTLLSFALLAQEITIKGRVLDENNVPLPGVTVLVE
                     GTSTGTTTSTDGTFSISINKSATGAILFSFMGYGKQRLELVEGKTYYEIALKPETTEM
                     EEVIVIGYGTQRKKDVSVSISTVKAEAVSSPAILSLDQSLQGQAAGVVVSQSTGKPGA
                     PVSIRIRGTSSINGNNEPLYVIDGLPIITNASDLTSGTIQGSDINPLSGINPNDIESI
                     QILKDASATAIYGARGANGVILITTKQGFKGQMQVSISSSLGFQQLSKKLEVLNARQL
                     AELGNAAVWEARKYYPDLAYNSAFAIPSRFGEGTDWQNQIFRTASLTNQQISVRGGGE
                     NSTYYMSANLAMQNGIIKNSDFGKGTFRVNLDNRISKSVKSGISINISRSINNGVTTG
                     VPNVASSVTAMALLFNPGQDVYDPSDESGYTYESNTLSRIPNPVAEINETKTKITTSR
                     VIADYYTDWNITSDLQYKLKAGVDAFFNDEQQFIPSYIKRGQGKGKGSNVGINGYTWL
                     VENTFTYMKDFNKHHLNLMVGQTAQKYVSESADIAVERFEDNRLGYYNLGLALDKTIN
                     TSYTTWAMLSGIGRLMYNYESKYYLTLSGRVDGSSKFGTSNKYGFFPSASMAWRVTGE
                     PFMESIEAVNDLKLRFSAGTVGNEGIPSGSSLSLLANRPYFFGEGTNAEVIGTYVYSL
                     ENEDLKWEVTTQYDAGMDLSLWQSRVEFIAEAYLKQTSDLLLYMPINTSSGFSYVWAN
                     VGDLSNKGMEFTLNTVNLKNEVKWSTAFNLSFNRNKVTNIDKSDKIYGNPIMNIIDWT
                     MISEGEPIGTIYGYKSDGIIQLDEDPSQIPFFVSKIPRYGDRKYIDKNGDGKLTTEDY
                     FKLGNTNPDFSFGLKNSFAYKNFTLSLYIQGDVGNSVVNFNLFQLESFDGAQNNLKVA
                     LERWTETNPSNKYPRANASPHGNVMSDVIVEDGSYIRLKEATLTYQFPKKIFAKTQLK
                     DLQLSISGRNLLTLTGYSGYDPEVSIFGGSVYGKGADYGAYPQSISVIFSISATF"
     gene            5556..7022
                     /locus_tag="PK839_07990"
     CDS             5556..7022
                     /locus_tag="PK839_07990"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOG72711.1"
                     /translation="MKRILIVLTILVLAGSGCEDFLDPDIRSEYNYNNYFQTEDDLIS
                     FANGIYGGLISWTWEGGGLFFNNFWVLQDLASDNAYETGPSIDMHDISNFTFDPNNTV
                     FFYIWQQCYEVINSCNILLQESENIQRYSSPTVKQHLQGEAYFIRGMLYFELVKLFGD
                     VPLYLAPTRSVQNTLIPRTPRAEVYNSIISDLSNAESLLVENPFQYRREGIPTTSAAS
                     ALLAKVYLTRGALNGNADDFNQCQIQLEKVLGKYSLENEFADIFKLKNANRNEIIWAV
                     NFSATLGEGWTTGQFIVRLMPTIVADNGVYNGQAWERPTDYLFNSYSSADKRKAATFI
                     TAFDDSLLNGAYIRKYWDQDAEGGRQNGESDADFIYLRYADVLLMYAEVLNEIYHSPT
                     TAAYNAINDVRNRANVGDLPAGLDYQSFKNAILQERQCEFVMEGQRWYDLVRMGKLYE
                     KVLLAKPNANIKQTHTLFPIPQRERYYNPNLTQNEGYQ"
     gene            7073..8830
                     /locus_tag="PK839_07995"
     CDS             7073..8830
                     /locus_tag="PK839_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009274966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HOG72712.1"
                     /translation="MLQSNFNKKPVIRVDEGYNSCFSGWESIFSQLKKKIEEDSDGIT
                     TVVVECYQGVFFDKLLLTFKDFIKPDRVITSQDFFLSEEEVKRITFPDVTDDRIFGFL
                     TRLHMEDFTDHLKVQYNRNSIKDAAKGVILVFGYGASLLVDEPTLLVYADMPRWEIQL
                     RMRSNQVDNLGIRNRDESIEAKYKRGFFVDWRVCDRLKKKLMNRWDYVLDTTRMDKPV
                     MVAAKTFEKALDKAISQPFSLMPYFDEGPWGGQWMKQVCNLDAEIDNYAWCFNCVPEE
                     NSLLFAFGETIFETPSINLVFARPRQLLGEAVHARFGDEFPIRFDFLDTMGGGNLSLQ
                     VHPLTEYIQEKFGIHYTQDESYYLLDVGEGASVYLGLKEGTNPDQMIFDLRKAQENDE
                     KFEAEKYVECWPANKHDHFLIPGGTVHCSGKNCLVLEISSTPYIFTFKLWDWDRLGLD
                     GKPRPINIDHGINVIQWDRTTSWTKNNLINRIRRVAQGDGWVEESTGLHEREFIETRR
                     HWFSKKVLHQTNGKVNVLNLVEGREVVVESPTHAFDPFIVHYAETFIVPAYVDEYTIS
                     PHGESQGKRCATIKAFVRT"
     gene            8846..10006
                     /locus_tag="PK839_08000"
     CDS             8846..10006
                     /locus_tag="PK839_08000"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOG72713.1"
                     /translation="MNSKHSLKFTLPIFMSFYVMGFVDLVGVATGYVKQDFGLSDSLA
                     QLLPSIVFVWFALFSIPTGIFQDRMGKRFTVNLAMIITGIGMLLPILSYSYLSTILAF
                     MIIGIGNTILQVSANPLLLDTSSDENKAANLSLSQFIKAISSMLGPIIVAALARYTGN
                     WRLVFPIYAAISFLSAFWLYSVKIVEVKPDKEPATLKTVFSLFKNPFIVTMILAIFFI
                     VGFDVSINSNIANFLKSKYAISLETASLGISVYFASLMAGRFLGAILLRKVSSKTFLN
                     ASVVLTVLGLVGILLSPVLGVTWVLIFITGFGFSNIFPLIFALTIERKPEYANEISGL
                     IIFAVCGGAVIPPIVGILTDALGVSSIIYVLLACVVYVSYATYYLTRKVLQR"
     gene            10020..11132
                     /locus_tag="PK839_08005"
     CDS             10020..11132
                     /locus_tag="PK839_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007658094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOG72714.1"
                     /translation="MAKSDINNDSRIILTLDAGGTNLVFSAIQGNKEIVQPRNYSTNA
                     SDLDKCLHTIIEGFREVYKLIPHKAAAISFAFPGPADYRNGIIGDLPNFPAFKGGVAL
                     GPMLMDEFEIPVFINNDGNLFAYGEALCGFLPELNARIISSGGIKQFKNLIGLTIGTG
                     FGCGVVIDKMMLLGDNSNDTGIHQTLNKFNPQWNAEESVSTRAIQRVYAQHSGHQFLS
                     QLMPKDIFDIAMGEQEGNVDAAKEAFRQFGEALGSSIANIVALIDGVVVIGGGIAAAW
                     PLYAPAMFKEINRQYKSWEETLFNRLDFEVYNLEEMSVFPDFALGRVRKIRVPYSNRE
                     IQYDECARVGIGLSKLGASKAIALGAYAYALQQLDS"
     gene            11391..13721
                     /locus_tag="PK839_08010"
     CDS             11391..13721
                     /locus_tag="PK839_08010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72715.1"
                     /translation="MKSYTIILKVSLLAILLGIMGCSENSTNPSRNIPTLEELSGIWV
                     SPDSVDMEPSIRNFRGQALVNRDLTSISWFASAPYSGGYHTGTMRINGHTPKLSSFRW
                     QPYQALRKGKFESIEILSTTRMLFENDAVMWQVELTNSGNEPINIKVDIDMIGFISKY
                     GGDWQWWYPYPKINGTETKRDEEVENVRKHLGEPIHEKEGYQWELVEGKPSYVKTILR
                     WPSDEEILNASKYVATGEGNVLLVSDKETDAKVAFGIVTPPNRIITKNSGGTACWELS
                     LAPGEKRVIKYLMTYGDDFELLKKNLNVWTTQYNSVFNSIKTSWEDKWAMIFKPNNSL
                     ISGCFPVLETDDKLARKVYYTSPLTMLYLTNTNLPQHNKVFLTGGPRWGATITFFWDI
                     TIWSTLWAVVDPVMMKEHISSWIKIDPSKFYGKDNFGGNGVGNGYSANYWALFQLIRD
                     YVVVTGDYDFLDESIEGKTVLQHLTDYALNWQKISIYGVEGCTDDVYKLADFGDDEWN
                     LLECVPTYKHIVPSFNVGYVWMMRETAKIYEKRGDLAKANELNKLASENIQRILKLYA
                     GNGVWNSLYPGGKMVEVRHCLDFSFLGKYIPNDIPPTIRNEMMDFLYRELMTDSWMRA
                     QSLSDIAAEQSDRCDHGPLGAFDGWPAEIMDALTQMGFPDKALDFYRAIEPVTYEGVW
                     SQAHELWGDEKKTKKARVRIAERGWHNRESSSGIAFSQVMLRDFFGFNPQLNGEVIQT
                     LHGLPLSGKLYHVFYQNEYYTIELKNGKSVMIKETN"
     gene            13723..15114
                     /locus_tag="PK839_08015"
     CDS             13723..15114
                     /locus_tag="PK839_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOG72716.1"
                     /translation="MMRLKVLKLVVFLICMIAEDRMILAQEFITPAQDQFNKFTNVAN
                     GWCAADATISLLLPDGKTVWLFGDTFIGQKTGDFSISPVNSRLINNSAIIEDNGLLSA
                     YYNGTMSSPSSLIPADGESYFWPEHAIIENDTIKIFALRVTHQNNETPGFDFRIGTTR
                     IANFKYPEMEYIGSTNIQSVTDTTMRFGACVLRSGNYTYIFGVKDTTSGGMTWPLPYL
                     ARCKESINEPWEFYAGNGLWTLRCEDSKPVGDRPMSESFYVYEKDKKFYLIMHEIWTV
                     GELYILEADSITGPWNRATSGGKETLFATITPQANLFSYNLFAHPQFQLDGKILISFN
                     VNTTNFSSIYSDTRNYRARFYWLSITDAVAADYPASISLLDCYSSVPIVHPTPMSSKI
                     YFQNATGNLRFRGIDAKSTITVYGVDGRIYLNRVIYCDSDLNINHLPHGILVVKLQGD
                     WGLETQKIINHNP"
     gene            15128..15886
                     /locus_tag="PK839_08020"
     CDS             15128..15886
                     /locus_tag="PK839_08020"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HOG72717.1"
                     /translation="MKTISISVFMLTWCYLGIAQETTKILIDSSSSKSMTMTELPDTV
                     VKNEAKAKELEEWNKAMRAYYYHRKEQFEMLPASNKDIIFLGNSITDQCEWHELFNNM
                     NVKNRGIGGDDTDGILERLGDITKSKPNKIFIMIGTNDLAYSKTINHIVENYRKIIQS
                     IRDSSPETKIYIQSILPTNDEIHTLRKNSDIMLVNQQLERISKEYSLTYIDLFSLFKT
                     DNNKLNPEYSLDGLHLNGKGYMVWKNAILKYVDE"
     gene            15974..>16795
                     /locus_tag="PK839_08025"
     CDS             15974..>16795
                     /locus_tag="PK839_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72718.1"
                     /translation="MRSSISSTLLAVFLFTGCCQEIKREVEENSLGQPEVLTFTSKPA
                     PEWTALMERTSGWFGADGIYSIPLDGVEDQSVEGKQTLFIFSDTYIGEVENNVPRPDN
                     IMVNNSVAWMTGVTPVKSAISFEFCKDMDGRPTSYFTPTNKKNDDDEYFWLGDGFINH
                     KKNNALYLFAYHVHKTGPNVFDFLATNVSILKIKNPTPQGIRTYEQIPTNLGFVHPSY
                     GRVYFGSGIFVNTKKAKAPYPDGYVYIYGIAEGKKSLLAARTKPENFEHFNKWTFW"
BASE COUNT         5074 a         3105 c         3647 g         4969 t
ORIGIN      
        1 aaaactagtt ttacatgtaa ttcatcttcc ccaaagctga atgttcaact cttttttctg
       61 aatatttttg aaatatgcta attttgtttg aacatttgaa ccatgattag atgaattata
      121 ctatagatca cagcagctcc ataccacttc atgtccaggt ggagaatctg cttcgggaga
      181 tgataaggga ccccgagttt gcaaacggaa aatttctacc caacgaggtt gatttagcaa
      241 agcgtctagg tatttctaga aacaccttga ggcaggctac caacaagtta acctatgaag
      301 gattgcttgt tcgaaagaag ggggtcggaa caattgttgc caccaaaagt gtcaataccc
      361 gtctcatgaa ttggcctagt ttttctcagg aaatgaaggc attaggcata aagattatta
      421 actatgaact acatgtatca tgggagcagg ctgataagaa aattgctgat ttttttagaa
      481 tctcggaagg aaagaaagtt cttaaacttg aacgcttaag gggggggccg gacggaccct
      541 tcgtttactt tatttcatac tttcatccaa gagttgggtt aacagggaat gaggatttta
      601 gtaagccttt gtacgaaatt cttgagaaga attacgctac tatagcccaa ctatccaagg
      661 aagagattat cgctattgct gctaatgaat ttttagcaca gaaattgggc atgaaacctg
      721 gcgatccaat tcttaagcgc aaacgatacg tttttgatcc tggaagcaga ccaatagagt
      781 ataatttggg tttctatcgc ggagatagct ttgtttacac tattgaaagc gaaagaaagt
      841 tatgaatatg ccacttttct gaatgacaca tttttgataa gtcgcaaata gtaaggcaga
      901 taataaaatt caattatttt attcaaaata tttgcttttt aaatttactt gttatataat
      961 tgtattttat tatgttcaaa catatgcaca taataatcac aaaccaatta actatttacc
     1021 aaaacctagc tgtcatgaaa agaaaaatga attttccact gcttttggtt tgcattaccc
     1081 tgttttgcat accatttgct atttcttcct gtaatgatga ggatgaagta aagaccaacg
     1141 ctaccattag cacatcttct acacctgttc ttgctgttgg tgaatctgct actgcaacta
     1201 tcaatatcgt tgccgaagga atcaaatcct ttaagtacta caaggttgtt gatgaggttc
     1261 gtgcggtaga atatgatgct ataaataatt tagtgaagga agggaataaa tatacatatt
     1321 cattttccta caccgttcag gaatttgacg acctccatac gctaggcttt gaatttgaga
     1381 tagttgataa agccaatgtt tcatacgtat tgggacttgt tgttaatact gaactatcgt
     1441 taaagtcttc atttgtaaaa ttcgattgga aggttactgc tagtgaatgg ctaggaatgg
     1501 acgttttagc tcctcatgat gctgcctata tatataggtt tcatgaggat ggcacttatg
     1561 aagttgatat gacacccgaa tatgccgata accttcatca cttttgctat tgggtcttta
     1621 aggaaactcc tgacaatggc gacacgttag cagtgatccg cttagttaga aagttaagat
     1681 cgggagaaac agctgttgat gaatattacg attaccgtat tgtaaacgcc acccaggctg
     1741 aaatgactat gtactgggat atccctgtgt ttggcatgtt taatattgcc aacacattta
     1801 ctagtcaacc aaaaggtgct tttcaacctt acgggactcc agagatggcc tctgaagttg
     1861 gaaatgatcc tgttatgagt tgtagcactg ttgaccctgc cctattaaca attcagaagt
     1921 aagtatagta gtagtatccc gggaaataat gctgttatcc cgggatatta cttttaaact
     1981 taatctcata gtaatacctg aatgcttgcc taagcgagga acgaaaatct agctcaatag
     2041 aaagagcgga ttataatttc agaaactatg gcactattat caccagcact ataattaatt
     2101 caaagtctaa gtggggctac tgtacttaag atttaatttt attttacctc atttttcatt
     2161 tttttgatgg gtgttgaact tatttcatca ttcataatac gctaaaccaa tagacattaa
     2221 atctttttga acatcatatg agcaatgtag ggtttgtttg acacataaga ttgattgtgt
     2281 ttctgttcct caatgtttta ggtagcatca caacttatta aatacaaaca gatgaaaaaa
     2341 attctgattt cgactttttt tactcttctg agttttgcat tgctggcaca agaaatcacc
     2401 ataaagggga gagttctgga tgaaaacaac gttccattgc ctggcgttac tgttttagtt
     2461 gaaggcacct ctaccggaac aacaactagt acagatggca cattctcaat atctataaac
     2521 aaaagcgcca ctggagctat tctattctcg tttatgggct atggtaagca gagactggaa
     2581 cttgttgaag gaaaaacgta ctatgaaata gcgttaaaac ccgaaacaac agaaatggaa
     2641 gaagttatag taattgggta cggaacgcaa agaaagaaag atgtatctgt ttccatttca
     2701 actgtaaaag cggaagccgt ttcatcgcca gccattctat cgctcgatca atccttacag
     2761 gggcaggctg ctggtgtagt tgtatcccag tcaacaggaa aacctggagc gccagtttct
     2821 attcgcatta gaggtacaag ttcaattaat ggcaacaatg aacctttata tgtaattgat
     2881 gggcttccaa ttattactaa tgcttccgat cttacttcag gaaccattca aggttccgat
     2941 attaatcccc tgtccggcat caacccaaac gatatagaat caatccaaat attaaaggac
     3001 gcttctgcca cagcaattta cggggctagg ggtgctaatg gggttattct tattacaact
     3061 aagcagggtt ttaagggaca aatgcaggtc agcattagct catctctagg atttcagcaa
     3121 ctttccaaaa aacttgaagt tttaaatgct cgccagcttg cagagcttgg gaatgctgct
     3181 gtttgggaag cccggaaata ctacccggat ttagcctata atagtgcatt tgccattcca
     3241 agccgttttg gagagggaac agactggcag aatcaaatat ttagaacggc aagcctcaca
     3301 aatcaacaaa tttctgttag gggaggaggt gaaaattcta catactacat gtcggcaaac
     3361 ctggctatgc agaatggaat tataaaaaat tccgattttg gaaagggaac ctttagggta
     3421 aacttagata ataggatttc aaaatcagtt aagagcggga taagcataaa tatttcgaga
     3481 agtataaata atggagtaac tacaggtgtg ccaaacgttg cctcaagtgt taccgcaatg
     3541 gcactactat ttaaccctgg gcaggatgtg tacgatcctt ctgatgagag tggctacaca
     3601 tatgaaagca ataccctgtc acgaattccc aacccggttg ctgagatcaa cgaaacgaaa
     3661 actaaaatta ccacgagcag ggtaattgct gattactata ccgactggaa tattactagt
     3721 gatctgcagt ataagcttaa ggcaggtgtt gatgcatttt ttaatgatga acagcaattt
     3781 atccctagct atattaaacg tgggcagggt aagggcaaag gatcaaatgt tggtattaat
     3841 gggtatacat ggcttgttga gaacaccttt acctacatga aggattttaa caaacatcat
     3901 ttgaatctaa tggttggaca aactgcacaa aagtatgttt cggaatcggc cgatattgca
     3961 gtagaaaggt ttgaggataa ccgtttaggc tactacaacc ttggattggc gttagataaa
     4021 acaataaata cttcgtacac aacatgggca atgctttcgg ggattggtcg gttgatgtac
     4081 aactacgaaa gtaagtacta ccttaccctg tcaggtcgtg tagatggctc ttctaagttt
     4141 ggtacctcca acaagtatgg ttttttccca tcggcttcca tggcatggag ggttactggt
     4201 gagcctttca tggagtcaat tgaagctgta aacgatttaa agttaaggtt tagtgcagga
     4261 acagttggaa acgagggtat tccttcaggt agttctctta gccttttggc caatcgtcct
     4321 tacttctttg gcgaagggac aaatgccgaa gtcataggca cttatgttta ttccctggaa
     4381 aatgaggatc taaagtggga ggtaactaca caatatgatg caggaatgga ccttagcctt
     4441 tggcaatcga gagttgaatt tattgcggag gcttatctga aacaaacgtc cgaccttctt
     4501 ctctatatgc ctattaatac atcttcgggt ttttcctacg tatgggcaaa tgttggtgat
     4561 ctttctaata aggggatgga atttacactg aataccgtca atctgaaaaa tgaggtaaag
     4621 tggagtactg cctttaacct atcgtttaat aggaataaag ttaccaacat tgacaaatca
     4681 gacaagattt acggtaatcc tattatgaat attatagact ggaccatgat aagcgagggg
     4741 gaacctatag gaacaattta tggctataag tctgacggaa taattcagct ggacgaagat
     4801 ccttcccaga taccattttt tgtttcaaaa attcctcgtt atggcgatag aaagtatata
     4861 gacaaaaatg gtgatggcaa gcttacaacc gaggactatt tcaagcttgg aaataccaac
     4921 ccggattttt cctttgggct gaaaaactcc ttcgcttaca aaaactttac actaagctta
     4981 tacattcaag gtgatgttgg aaatagtgtg gtaaatttta acctctttca gctggaaagt
     5041 tttgacggag cacaaaataa cctgaaggtg gctcttgaac ggtggacgga aacaaaccct
     5101 tcaaataaat atcctagggc aaatgcctcc cctcatggca acgtaatgtc cgatgtaatt
     5161 gttgaagatg gctcgtatat aaggcttaag gaggcaaccc ttacctacca gttccctaag
     5221 aaaatatttg ctaaaacaca gctcaaagat ttgcaacttt ctatcagtgg gcgaaatctc
     5281 cttacactca caggatattc gggatacgat ccagaagttt caatatttgg tggaagtgtt
     5341 tatggtaaag gtgccgacta tggggcatac cctcagtcaa tatcggttat attcagcatt
     5401 agtgcaactt tttagccact aacgcaccac cagtcgatct tgttggcttt tagtaaaaga
     5461 accattttag taaccactta cattatgatt tcgcttcatt cacctgttga catattcatt
     5521 gcttctttaa ctgtttatgc ctaaaagata aaagtatgaa aagaattcta atagttttaa
     5581 ccatattggt tttggctggt agcggttgcg aggatttttt agatccagac atacgctcgg
     5641 agtataacta caacaactat ttccaaaccg aggatgattt aataagcttt gctaatggta
     5701 tttatggagg gctgatttcc tggacatggg aagggggtgg cttgtttttc aacaactttt
     5761 gggtgcttca ggatttggct tctgataacg cttacgaaac tggcccatcc attgatatgc
     5821 atgatatttc taactttact ttcgatccaa acaacacagt ttttttctac atctggcaac
     5881 aatgctatga ggtgataaac tcatgcaata ttctacttca ggaatcggaa aacatccaga
     5941 gatattctag ccctaccgtt aagcaacatc ttcaaggaga agcttacttt attaggggaa
     6001 tgctgtattt tgaactagta aaactttttg gggacgttcc cctttaccta gctcccacaa
     6061 ggagtgttca aaacacttta ataccccgca caccgagggc tgaggtttac aacagcatca
     6121 tcagcgattt aagtaatgca gaatctcttt tggttgaaaa tccctttcag tatagaaggg
     6181 aagggatacc tacaaccagt gcggcatcag ccctactagc taaggtttac ctgacccgag
     6241 gagcactgaa tggaaatgca gatgatttta accaatgtca gattcagctt gaaaaagtct
     6301 tgggtaaata ttctcttgag aatgaatttg ctgatatttt taaacttaaa aatgccaata
     6361 gaaacgagat aatatgggca gttaacttta gcgcaacttt aggtgagggt tggacaacag
     6421 gacagtttat tgtacgtctg atgcctacta ttgttgccga taacggggtg tataatggtc
     6481 aggcttggga aaggccaact gactacctct tcaacagtta tagcagcgcc gacaagcgga
     6541 aagcggcaac atttattact gcttttgacg acagcctgct taatggtgca tacataagaa
     6601 aatactggga tcaggatgct gaaggtggaa gacaaaacgg agaatctgat gcggatttta
     6661 tttaccttcg ctatgctgat gtactgctta tgtatgctga ggtacttaac gaaatttatc
     6721 atagccctac aaccgcagca tacaatgcta ttaacgatgt aagaaatcgt gcaaatgttg
     6781 gcgatctccc cgccgggctt gattaccaat catttaaaaa tgctattctg caggaaaggc
     6841 aatgtgagtt tgttatggaa ggacaaaggt ggtacgattt agttcgtatg ggaaaactgt
     6901 acgaaaaagt tcttcttgcc aaaccaaatg ccaacatcaa acaaacacat acgctatttc
     6961 caatacctca aagggaaaga tattataacc ccaatcttac tcagaatgag gggtaccaat
     7021 agtaagttgt aagtagctaa ctaggtatat tattaacttt taatacacat tgattttgca
     7081 atcgaatttt aataaaaagc ctgtaattag agtagatgaa ggctataatt cttgttttag
     7141 tgggtgggaa agtattttta gccaacttaa gaaaaaaatt gaagaggact cggatggtat
     7201 aactacagta gtagttgagt gttaccaggg tgtgtttttt gacaagctac tacttacttt
     7261 caaggatttt ataaagcctg acagggtgat taccagccaa gatttttttc tttcagaaga
     7321 agaggttaaa cggataacct ttccagatgt aaccgacgac cggatttttg ggtttcttac
     7381 ccgtttacat atggaagatt ttaccgatca cctaaaagtt cagtacaaca ggaacagtat
     7441 taaagatgcg gcaaagggag ttatactggt tttcggatat ggggcatcat tactcgttga
     7501 tgaacccacg ttgctggttt atgccgatat gccaagatgg gaaatccagc tacggatgcg
     7561 atctaatcag gttgataatc ttggaattag aaacagggac gagagtattg aagccaagta
     7621 taaaagggga ttttttgttg actggcgagt ttgtgatagg ctgaagaaga agctaatgaa
     7681 ccgatgggat tatgttctgg atactacacg aatggataag cctgtaatgg ttgcagctaa
     7741 aactttcgaa aaagctctag ataaagctat ttctcaacct ttcagcctaa tgccctactt
     7801 tgacgagggg ccttggggtg gccaatggat gaagcaagtt tgcaatctgg acgccgaaat
     7861 tgataattac gcatggtgct ttaactgtgt tcctgaagag aacagcctcc tttttgcctt
     7921 tggagagaca atttttgaaa ctccttcaat aaatcttgtt tttgcacgcc ccaggcaact
     7981 tttgggagag gctgtacatg cacggttcgg agatgagttc cctattcggt ttgatttctt
     8041 agatactatg ggtggaggta atcttagcct tcaagttcat ccgcttactg agtacattca
     8101 agaaaagttt ggaattcact acactcagga cgagagctac tatttgcttg atgtaggaga
     8161 gggtgcatct gtctatctgg gtcttaagga ggggactaat cccgatcaga tgattttcga
     8221 cttgcgaaaa gcccaagaga atgacgaaaa gtttgaggct gaaaaatatg tggagtgttg
     8281 gccagctaat aagcatgatc attttttaat accaggaggc acggttcact gttcaggaaa
     8341 gaactgcctt gtgcttgaaa ttagttctac cccctatatt tttacgttta agctttggga
     8401 ctgggataga cttggtttag atggaaaacc ccgtcccatc aatattgacc atgggataaa
     8461 cgtcattcaa tgggacagaa ccaccagctg gacaaaaaac aaccttatca accgcattag
     8521 gagggtagcc caaggcgacg gttgggttga ggagtcaacc ggactccatg agcgtgaatt
     8581 tattgaaacc agaagacatt ggttttcaaa aaaggtatta catcaaacaa atgggaaagt
     8641 aaatgtgcta aacctcgttg aaggtagaga ggttgtcgta gaaagcccca ctcatgcttt
     8701 cgacccattt attgtgcatt acgcggagac ttttattgtt ccagcctatg tggatgagta
     8761 tactataagt ccacatgggg aatcgcaagg aaagcgttgt gctaccataa aggcatttgt
     8821 acgaacttaa aaaccatata gcggtatgaa tagtaagcac tccttaaaat ttaccttacc
     8881 aattttcatg tccttttacg tcatggggtt tgttgatttg gtgggtgtgg caaccggtta
     8941 tgtaaagcag gatttcgggc tttcggatag cctagcccaa cttcttccga gcattgtttt
     9001 tgtgtggttt gcattattct caatccccac cggaattttt caggatagaa tgggtaagcg
     9061 attcactgta aacttggcaa tgataataac cggtatcggt atgctgctgc cgattctttc
     9121 ttactcatac ttaagcacta tccttgcttt tatgattatt ggcattggaa ataccattct
     9181 tcaggtttca gccaacccac ttttacttga tacatcttct gacgagaaca aagcagcaaa
     9241 tcttagtctg tctcaattta taaaggctat ttcttccatg ttaggcccca tcattgttgc
     9301 cgcattggca agatatacag gaaactggag acttgtattt cccatttacg cggccatatc
     9361 gtttctgtca gccttttggc tttatagtgt gaagattgtg gaggtaaaac ccgacaagga
     9421 acccgcaacc cttaaaacgg ttttttccct tttcaaaaac cctttcatcg ttaccatgat
     9481 cttggccatt ttctttatcg tgggattcga tgtttctatc aattcgaata ttgccaattt
     9541 tctgaagagt aaatacgcca taagccttga aacggctagc ctgggtatca gcgtatattt
     9601 tgcctccctg atggcagggc gatttctggg ggccatcctg cttagaaaag tcagctccaa
     9661 aacctttctg aatgccagtg ttgtgcttac cgttttaggg ttagttggca ttctcctttc
     9721 gcctgtttta ggcgtaacat gggtgctaat ttttattacc gggtttggct tctcaaatat
     9781 tttcccgcta atttttgccc ttactataga aaggaaaccg gagtatgcca atgaaatttc
     9841 cgggttaata atttttgcag tctgtggcgg ggcagtgatt cctcctattg ttggaatatt
     9901 gactgatgcc cttggagttt cttctatcat atacgttctc ttagcatgtg ttgtttacgt
     9961 ttcatacgct acctactacc tcacccggaa agttttacaa cgttaattgt gttacgaaca
    10021 tggcaaagtc agatattaat aatgattcaa ggattatcct aactctggac gcgggaggta
    10081 caaacctggt tttttctgct attcagggaa ataaggagat tgttcagccc agaaattact
    10141 cgaccaatgc aagcgattta gacaaatgcc ttcatacaat tattgagggt tttagagagg
    10201 tttacaagtt aatcccccat aaagcagcgg ctattagttt tgcatttcct ggacctgccg
    10261 attacagaaa tgggattatt ggagatcttc caaattttcc tgcttttaag ggaggtgtgg
    10321 cattaggtcc catgttgatg gatgaatttg agattcctgt ttttatcaat aatgatggta
    10381 acttatttgc atatggcgag gccctatgcg ggttcctacc agagctgaat gcaagaatta
    10441 ttagtagtgg gggaatcaaa cagtttaaaa accttatagg attgacgata ggaacaggct
    10501 tcggttgcgg agtagttata gataaaatga tgcttttggg agataactca aacgacaccg
    10561 gcattcacca aacattaaac aaatttaacc ctcagtggaa tgccgaagag agcgttagca
    10621 caagggcgat tcagagagtt tacgcacaac actcgggcca tcagttctta agccagctta
    10681 tgccaaagga tattttcgac attgccatgg gtgaacaaga agggaatgtt gatgccgcta
    10741 aagaggcttt tagacaattt ggtgaagctt taggtagttc catagctaac attgttgcac
    10801 ttattgacgg agtggttgta attggcggtg gaattgctgc ggcttggcca ctttatgctc
    10861 ccgccatgtt taaagaaatt aataggcagt ataaaagctg ggaggaaacg ctgtttaatc
    10921 gtttagattt tgaagtgtat aacctcgagg aaatgagtgt attcccggat tttgcacttg
    10981 gcagagtaag gaaaataaga gtgccgtata gtaatagaga gatacagtac gacgagtgtg
    11041 ccagggttgg tatcggactg tctaaattgg gcgccagtaa ggcaattgca ctcggtgctt
    11101 acgcttatgc tctacaacaa ttagatagct aaattttggg ctgatgctca tgtttttaaa
    11161 cccgatttat gcttaaaagt aataatcatt atcaagcttt catgtagttg tttctgtaac
    11221 cgaatgagag tggctacacc caagctaata tttaccatgg atattttgcc tactggtgag
    11281 ttgttgtttt ttcggatcgc tggttgtttt ggggttggtt tatcatgtta aaagacttaa
    11341 agcgcagaaa tatagttcat tagtataaac gttataaact tgctgtacag atgaaatcat
    11401 atacaataat attaaaagtt tctcttttag ctattcttct gggtattatg ggatgttccg
    11461 aaaacagcac caatcctagt agaaatattc ccaccctgga agaactatct ggaatttggg
    11521 tttctccaga ttctgtagat atggagccat ctattagaaa ttttcgtggc caagcattag
    11581 taaacaggga cttaacctca ataagttggt ttgcatcggc gccatactcc ggaggttatc
    11641 acaccgggac aatgaggata aatggtcata ccccaaaatt aagttctttt cgatggcagc
    11701 cataccaggc cttgcgcaaa gggaagtttg aaagtattga aatcctcagc acaactcgaa
    11761 tgcttttcga aaatgacgcc gtaatgtggc aggtggaatt aacaaattct ggaaatgaac
    11821 ccattaatat taaggtggat attgatatga ttggattcat tagcaagtat ggtggtgact
    11881 ggcaatggtg gtatccctac ccaaaaatta atggaaccga aacaaaacgg gatgaggagg
    11941 tggagaatgt taggaaacat ttgggtgaac ctattcatga aaaagagggg taccagtggg
    12001 agttagttga aggcaagccc tcctatgtta aaacgatact gaggtggcca agcgatgaag
    12061 agatactgaa tgctagtaag tatgttgcca ctggcgaagg taatgttttg ttagtgagtg
    12121 ataaagaaac tgatgctaaa gttgcctttg gtatagttac acctcccaac aggattatta
    12181 caaagaattc cggcggtacg gcttgttggg aattatcact agcaccaggg gagaagaggg
    12241 taattaaata tcttatgaca tatggtgatg attttgagtt gctgaagaaa aatctaaacg
    12301 tatggactac tcaatacaac agcgttttca acagtatcaa gaccagctgg gaggataaat
    12361 gggctatgat tttcaagcct aacaattctt tgatatcggg ttgctttccg gttcttgaaa
    12421 cagacgataa gttggccaga aaggtttact atactagccc tcttaccatg ctttacctta
    12481 caaatactaa cctcccacag cataacaagg tattcctgac gggtggtcca cgatggggtg
    12541 caaccattac atttttttgg gacataacta tatggtcaac cctatgggcg gttgtggatc
    12601 ctgtaatgat gaaggaacac atttcatcat ggattaaaat tgatccaagt aagttttatg
    12661 gaaaggataa ttttggcgga aatggtgtag gcaacgggta ttctgcaaac tactgggcac
    12721 tttttcagct cattcgtgat tacgttgtgg ttactggtga ttatgatttt cttgacgagt
    12781 ccattgaagg gaaaaccgtt cttcaacacc taaccgatta tgccttaaac tggcagaaaa
    12841 tatctattta tggagttgaa ggttgtacgg atgatgttta taaactggcc gattttggcg
    12901 atgatgaatg gaatttgctg gagtgtgtgc ccacctataa gcacattgtt ccatctttta
    12961 atgtagggta tgtatggatg atgcgcgaga cagccaaaat ttatgagaaa agaggtgatt
    13021 tggccaaagc aaatgagttg aacaaattgg catccgaaaa tattcagcga attcttaaac
    13081 tatacgctgg gaatggggtt tggaatagcc tttaccctgg gggaaaaatg gtagaggtac
    13141 gccattgttt agacttttca ttcttaggga aatacattcc aaacgatatt cccccgacaa
    13201 ttagaaatga aatgatggat tttctttaca gggagctaat gactgatagc tggatgcgtg
    13261 cccaatcgtt aagcgacatt gctgccgagc aatctgaccg atgcgatcat ggtccattgg
    13321 gcgcttttga tgggtggcct gccgaaataa tggatgccct tacccaaatg gggtttccag
    13381 ataaggcact tgatttttat cgagctattg aacctgttac ctatgagggt gtttggtcgc
    13441 aagcccatga actctggggt gatgagaaga aaactaaaaa ggccagagtg cgaattgctg
    13501 aaagaggttg gcataaccga gaatcatcgt caggaatagc attctcacag gtaatgctac
    13561 gcgatttttt cggatttaac ccgcagctta acggtgaagt tattcagact ttacatggtt
    13621 tgccgttaag tggtaaactt taccatgttt tctatcaaaa tgagtactac actattgaac
    13681 ttaaaaatgg gaagtcggta atgattaaag aaactaacta gcatgatgcg gctgaaagta
    13741 ttaaaattag ttgttttcct gatatgtatg attgctgaag acaggatgat tctggcgcag
    13801 gagtttatta ccccagctca ggatcaattt aataagttta ccaacgttgc caacggttgg
    13861 tgtgctgccg atgccaccat ttcgcttctt ctgcccgatg gtaaaaccgt atggctgttt
    13921 ggcgatactt ttattggcca aaaaacgggc gatttcagca ttagtcctgt caattccagg
    13981 ctgataaata actcggcaat tattgaagac aatggcttgc tttcagcata ctataacggc
    14041 actatgtcca gcccatcttc attaataccc gcagatggag agagctactt ttggcccgag
    14101 catgcgatta ttgagaatga taccatcaag atatttgcac ttagggttac acaccagaat
    14161 aatgagactc ccggttttga ttttcgtatt ggtactactc gaatcgccaa ctttaaatat
    14221 cctgaaatgg agtatatcgg gtctactaat attcaaagcg ttaccgacac aacaatgcgt
    14281 tttggtgcat gtgttcttag aagcgggaac tacacttaca tttttggtgt caaggatact
    14341 acctcgggag gaatgacatg gccattaccc taccttgcaa gatgcaagga aagcataaat
    14401 gagccatggg aattttatgc ggggaatggc ctatggacgc ttcgctgtga ggattctaaa
    14461 cctgtaggcg atcgtccgat gagtgaatcc ttttatgtat atgaaaagga taaaaagttt
    14521 tacctgatta tgcatgaaat ttggacagtt ggagaacttt acattctgga ggccgacagt
    14581 ataaccggac catggaacag ggctacaagc ggagggaagg aaactttgtt tgcaaccata
    14641 acacctcaag ccaatctttt ttcctacaac ctttttgcac atccccaatt tcagctggac
    14701 ggtaaaatac ttatttcatt caacgtaaac accactaatt tttcctcgat ttattccgac
    14761 acgcggaact accgagctcg gttctactgg ctttccatca ctgatgctgt agctgctgat
    14821 tacccggcca gcatttcgtt gctcgattgt tatagtagcg ttcctattgt tcatccaaca
    14881 ccaatgtcat ccaaaattta ctttcaaaac gcaaccggaa atcttaggtt taggggaata
    14941 gacgctaaat ctaccataac tgtatatgga gttgacggac gaatttacct taatagggtg
    15001 atttattgcg attctgacct taatataaac cacctacccc atggtatcct ggttgtaaag
    15061 ttacaggggg attggggctt ggaaacacaa aaaataatta accataaccc ttaaaattag
    15121 ctatgatatg aagacaatta gtatttcagt attcatgcta acctggtgct atctgggaat
    15181 agcgcaggaa acaacaaaga tcttgattga ttcttcgtca agtaagtcca tgaccatgac
    15241 agagcttccc gatactgtag ttaaaaatga agcaaaagca aaggagcttg aagaatggaa
    15301 caaggcgatg agggcctact actaccaccg taaagagcaa ttcgaaatgc ttccagcttc
    15361 caataaagac attatttttt tagggaatag tattaccgac cagtgtgaat ggcatgagct
    15421 attcaataac atgaatgtca aaaacagggg tattggtgga gatgatactg atggaattct
    15481 tgaaaggcta ggcgatataa caaaatcgaa gcctaataaa atatttatca tgattggcac
    15541 aaacgatctg gcctactcta aaacaattaa ccacattgtt gagaactatc ggaagattat
    15601 tcaaagtatt agggattctt cgcctgaaac aaaaatatac attcagagca ttttacctac
    15661 taatgatgaa attcacaccc ttaggaaaaa ttcggatata atgctggtta accaacagct
    15721 cgagcgaatt tcgaaagaat actcacttac ctatatcgac cttttttcgc tatttaaaac
    15781 agacaataat aagttaaatc ccgagtacag ccttgatggg cttcatctaa atggtaaagg
    15841 ctatatggtg tggaagaatg ccatacttaa atatgtggat gagtagcctg aggtgtaaaa
    15901 gtggtggttc atatggtgcc acactgccag gtatagctag gtggaaggtt tttcaattaa
    15961 aatttgcagg caaatgagga gttcgatttc atcaacttta ctggcagttt ttttgtttac
    16021 cggttgctgc caggagataa agagggaggt agaagaaaat tcattgggtc agcccgaggt
    16081 cctaaccttt acaagcaaac ctgctcctga atggactgct ttgatggagc gcacctctgg
    16141 ttggtttggt gccgatggaa tctactctat tccacttgat ggagttgaag accaaagtgt
    16201 ggagggaaaa caaaccctat tcatctttag cgacacctat attggtgagg ttgaaaataa
    16261 tgtgcccaga ccagacaaca tcatggttaa caactctgta gcatggatga ccggggttac
    16321 acccgttaaa tcggccattt catttgagtt ctgtaaggat atggatggca ggccaacttc
    16381 atactttact ccaaccaata agaaaaacga tgatgacgag tatttctggt taggtgatgg
    16441 ctttatcaac cataagaaga acaacgcact ctacctcttt gcctaccatg tgcataaaac
    16501 aggtccgaac gtttttgatt ttctggcaac caacgtatca attctaaaaa ttaaaaatcc
    16561 tactccgcag ggtattagaa cctacgaaca aattcctaca aacctgggtt ttgttcaccc
    16621 aagctacgga agagtttact ttggaagcgg aatttttgtt aatacaaaaa aagccaaagc
    16681 gccgtacccc gatggatacg tatatattta cggaattgct gagggtaaaa aatccctcct
    16741 agcagcaaga acaaaacccg aaaactttga gcatttcaat aaatggacat tttgg
//
LOCUS       DAOSHM010000050        16687 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_2811_length_16687_cov_21.969577, whole genome shotgun
            sequence.
ACCESSION   DAOSHM010000050 DAOSHM010000000
VERSION     DAOSHM010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 16687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16687
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2811_length_16687_cov_21.969577"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            97..1245
                     /locus_tag="PK839_08030"
     CDS             97..1245
                     /locus_tag="PK839_08030"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit"
                     /protein_id="HOG72719.1"
                     /translation="MLFSDIVGQEKVKERLIQLVRENRVPHANLFVGPPGAGKLPLAV
                     AFAQYIMCENRGESDSCGVCPSCRKVAKLVHPDLHFVFPTKTKEKSDEGADENSEKPD
                     DTISTWRSAIIANPYFGENDWYEMIGMENKQGIISTATASEIIRKLGLKSFENEFKVI
                     IIWLPERMNNQAANKLLKLIEEPPEKTIFLLVSENPTHILKTILSRTQQVMVPPINRE
                     SVALALHDKYNLSPELAHDISRIANGNFYEAIRLKDQESNYFFTSYRKLMRLCYSKNV
                     LGLLDWVDETASLGREDLKHLLVYSLRLTRETLVLNMGVEEVSYLMGEEEAFCHDFSP
                     FITTNNVFSITDELVTAFECITQNGNPTVVLTHMTMRMVKLIPNKKAA"
     gene            1299..2459
                     /gene="ricT"
                     /locus_tag="PK839_08035"
     CDS             1299..2459
                     /gene="ricT"
                     /locus_tag="PK839_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HOG72720.1"
                     /translation="MEKNIGSRGGVITVNEASEHSVCSSKGCAKLKVHDWMADLPMPL
                     QQSDIIEVQFKNTRKGFYRNANQLVLRQGDIVAVEATPGHDIGVVTLTGWLVERQMKR
                     VGVDPKTTEFKKVYRKAKQADIEKWIEAIALENSTMIRARKIAEEQRLNMKIGDVEYQ
                     GDKTKAIFYYIADERVDFRELIKILAEEFRVKIEMRQIGARQEAGRIGGIGPCGRELC
                     CSSWISHFASVSTNSAKVQELSLNPQKLAGQCGKLKCCLNYELDSYMDARKDFPDTSI
                     ALETADATYYHFKTDIFRRSMWYGSEPSSSVNLIEVSVDRVNEIIALNRKGIKADKLL
                     ENTTSDQNNSRPNGFTDGLLDEGSLTRFDKKGGKTAKRKKSRNRNRTRPSNE"
     gene            2452..2931
                     /locus_tag="PK839_08040"
     CDS             2452..2931
                     /locus_tag="PK839_08040"
                     /inference="COORDINATES: protein motif:HMM:TIGR03511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldH"
                     /protein_id="HOG72721.1"
                     /translation="MSKSLPIAFLAATLTLACCNRDAVFDSYVAIPRETWSADSLASF
                     NIAIPDTSQSYDIMINLRNSTDYPFQNLFLFISIYSPNGGFVRDTFECFLADDNGRWT
                     GRGSGRIHDNRFIYREGVKFYQPGSYRFDLQQAMRVEDLRGVVDVGIRIEQSQQNFK"
     gene            2986..3684
                     /gene="trmB"
                     /locus_tag="PK839_08045"
     CDS             2986..3684
                     /gene="trmB"
                     /locus_tag="PK839_08045"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009132982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HOG72722.1"
                     /translation="MGKNKLKRFAENETFSNLFQPPLEEVLNDKFELKGKWGNSYFKN
                     INPLVLELGCGKGEYTVSLAGMNPQKNFIGIDIKGARIWRGAKTALENKLNNVAFVRT
                     RIEHICSIFAQNEVDELWITFPDPQEREIKAKKRLTSSRFLNLYSGFLKPNAIVHLKT
                     DNRQLFDYTKALVEQNKFNVLICTTDLYANFTNDPVLGIKTFYEQQFLGEGLPITYLR
                     FVLNHPGPYHEPIR"
     gene            3668..4012
                     /locus_tag="PK839_08050"
     CDS             3668..4012
                     /locus_tag="PK839_08050"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:TIGR00589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HOG72723.1"
                     /translation="MNQSGNDNTGFFEKVYEVTRMIPYGRITTYGAIARYLGSAQSAR
                     MVGWALNSCHSQPDWVPAHRVVNRNGQLTGKVHFPGSNTMQQLLESEGIRVEDDTVLE
                     FKKFFWNPATEL"
     gene            4128..5231
                     /locus_tag="PK839_08055"
     CDS             4128..5231
                     /locus_tag="PK839_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004584185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HOG72724.1"
                     /translation="MNLTTDQVLEVLNQVVHPESGQSIVSMGMVKDLTASDRRISFTL
                     KLTKKNDPFASSLKKTAQRLLTERVGQQVEIDIQVVADIPERISPLKTKASVSRVKNI
                     VAIASGKGGVGKSTIAANLAVSVAKSGARVGLIDADIYGPSIPKMFNVENVQPEVMKE
                     DEMELIVPVERYGVKLLSIGFFVDPNNALIWRGPMATSALKQLIHQGAWGELDYLFID
                     MPPGTSDIHLTLVQEVAVTGAVIVSTPQDVALADAVKGISMFGSQGINVPVLGLVENM
                     AWFTPEELPENRYYIFGKDGCKKLAERMKLPLLGQIPLVQSIREGGDFGEPVALHSSV
                     VAKAFDELATNLIRQVEIRNRELPPTTIVNVKH"
     gene            5261..5515
                     /locus_tag="PK839_08060"
     CDS             5261..5515
                     /locus_tag="PK839_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HOG72725.1"
                     /translation="MTSKEEIVTKVAAAIDVIRPFLQNDGGDISLVEVTDDNTVRVKL
                     HGACGSCPYSLMTLKNGVEQAIMRDVPEIRGVEAVNLNIP"
     gene            5745..6872
                     /locus_tag="PK839_08065"
     CDS             5745..6872
                     /locus_tag="PK839_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72726.1"
                     /translation="MRYVLLFTLMLVVASVAAQEPVTLKFTRPSKVQASASKIKLKVQ
                     GNEYVLKNGASISINVTPEYGKSLLINAGDQTAYYLKPKPGETYEFEVGFKFAGPYIL
                     LLSGEEAKPGEAVQVTDTTAKPEGWETNLKVDRDNMGAGITLQKTDQSEAIRQEWLAR
                     GGKIKYQSVVFNGTYFGMDMDMEGVDANRIDGYGGGISFYQNRINLKIPAYKTGASSW
                     NSFNIGFGYDMLVYAYKFDMKMDPITTKMDVVTITMPFSANIGWTLGLGKFIDEGNWK
                     GVALTLRYRPSIMFNFTNSTVKMTSTDPHISGSTSTSTDSNVQFNAGGVGFDVDFSNF
                     SSTMEKLAPKPKLKLSFFVMPPVKDMPLFISFGLGITTYSR"
     gene            7087..7548
                     /locus_tag="PK839_08070"
     CDS             7087..7548
                     /locus_tag="PK839_08070"
                     /inference="COORDINATES: protein motif:HMM:NF015678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HOG72727.1"
                     /translation="MRTLGNILWHFPFLGFLTALGTFLIGGLLVITVIGSPLGLGLIQ
                     LSKFLLAPFSNAMVDKTDLQADQNKAWKTYGIIVRILYFPIGLFLVVVNIFQIAGLFI
                     SIVGIPVALVLAKSIGTYFNPVNKVCVSKAVADELSSRKAKAEVDKAFATK"
     gene            7748..8317
                     /locus_tag="PK839_08075"
     CDS             7748..8317
                     /locus_tag="PK839_08075"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HOG72728.1"
                     /translation="MLLLATLMCPALYAIPDTSRVALLVIDIQDFYFPGGKSELVNPL
                     PAAKNAALLVEYFRSHQLPVIYVKHQSAEQEEINEMVKPLADEPIFTKTEVNCFVGTQ
                     LYDYLVGQQISKLVICGMQTHLCVEAAVRAASDYGFKVTLIHDACATRDVKWGNTTIP
                     ANMVHLSTLSTLQNYARILSTEECLKANP"
     gene            complement(8330..9493)
                     /locus_tag="PK839_08080"
     CDS             complement(8330..9493)
                     /locus_tag="PK839_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOG72729.1"
                     /translation="MAKILVIDDEKAIRSTLKEILEFESHSVDLAEDGPTGIEMFGSG
                     CYDIVLCDIKMPQMDGIEVLEKLQEQAADVPVVMISGHGNIDTAVESIKKGAYDFIEK
                     PLDLNRLLITIRNATDKTTLIQEAKILKRKVNKAYDIVGESPAIVKVKEMIDRVAPTE
                     ARVLITGPNGTGKELVARWLHEKSNRSDMPFVEVNCAAIPSELIESELFGHEKGAFTS
                     AIKQRKGKFEQADGGTLFLDEIGDMSLSAQSKVLRALQENKISRVGSDKDIPVNVRVI
                     AATNKNLTHEIQNGNFREDLYHRLSVIIIHVPPLSERVEDIPLLAEHFNQLICSEYGI
                     PPKVFRPDAVDELKRLPWTGNVRELRNVVERLIILCDKDITGGDVKAFASPAF"
     gene            complement(9540..10469)
                     /locus_tag="PK839_08085"
     CDS             complement(9540..10469)
                     /locus_tag="PK839_08085"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72730.1"
                     /translation="MNVAIIDMGTNTFNLLVARFNQEERPEIIYEEKLPVRLGEGGLN
                     RKIITPEAWQRGLDAINTHMAKAKSLGAGKFFAFATSATRDASNGTNFVEDIRTSFGL
                     NVEIIDGNREATLVYKGVREALDLGNTCSLILDIGGGSNEIILGNTSEPFWLHSFNLG
                     VSRLMQQFQLSDPITQKQLRALEEFINVEVQTLFDAARRYVPVRLVGSSGSFDSIRSI
                     MEHSGSIAPGSEPWAEIPLERYFELHNFFITSTHDQRMALKGLDPMRVDLIVPASVFI
                     NLIIQKIGINKLFQSSFALKEGFMAELQSSHAF"
     gene            complement(10481..11122)
                     /locus_tag="PK839_08090"
     CDS             complement(10481..11122)
                     /locus_tag="PK839_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOG72731.1"
                     /translation="MDNDLQQVYQSVKFACRGDSTGHDWWHILRVVKLALFISERENA
                     NPFTVELAALLHDVDDWKLNRNTEGCPVALRILHESGYSDDEARRVALIIGQVSFKGA
                     GVDTTPSTIEAKVVQDADRLDAIGAIGVARAFAYGGARQRPMYIPDLKPQQHHSFDEY
                     QHAHGSTINHFYEKLLLLKDRLNTQTARQIAVERHQFMELFLTQFFKEWSVLD"
     gene            complement(11221..12573)
                     /locus_tag="PK839_08095"
     CDS             complement(11221..12573)
                     /locus_tag="PK839_08095"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG72732.1"
                     /translation="MKQMFLILQREFMTRVRKKSFIFTTLLSPLFFAAMVILPTYFAT
                     MEDTEVRNIAVIDNTQHFGSAIPQTEYIKITTITDTPAEKVKNSLEESGYYALLTIDS
                     LPGSIAPALTIYSPKQPSIEVISHIENSMEKEIETRKLMTYNIQDLDKILKDVKTKVN
                     VRSIKISKDGEEKESNTLITMGIAYILSFLMYMMVMLMGNQVMQGVIEEKNNRVVELL
                     VSSVKPVQLMMGKIFGIAAVGLLQIAIWIVLTAGLAGVGLNLISEKSGKANMEQVQIA
                     NQSIMANQGAQSADVTAAISETNTDNFLQALKNQNYGLIIGGFIFFFLMGYLLYAAMY
                     AAVGSAVENVSDTQQLVMPITIPLILAIFVMIVGIKSPDGSLAFWFSMIPFTSPIVML
                     TRLPFGVPVWQLILSAALLIGTFVVTVWLAAKIYRVGILMYGKKYSWKEMIKWIRYRS
                     "
     gene            complement(12598..13539)
                     /locus_tag="PK839_08100"
     CDS             complement(12598..13539)
                     /locus_tag="PK839_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG72733.1"
                     /translation="MNLLETHGVEKRFSNHLALDDISIAIPKGSIYGLLGPNGAGKTT
                     LIRIINQITGPDKGEVMFDGRKIEPNDIYRIGYLPEERGLYKKMKVGEQALFFSQLKG
                     LSRQDATKSLKYWFEKFEIQSWWDKKIEELSKGMAQKVQFIVTVIHKPELLIFDEPFS
                     GFDPINTQLITDEILELKKNGTTIIFSTHNMESVEQLCDHITLINKSKNILSGQIDQV
                     RANYGSNVFEVAFKGNLETLRQNLNGTFDIFNVNTEKAIPRVQVRLQSASDSNKLLQS
                     LISSVEVLSFNPVVPSMHDIFIRVVQEYNKAHGLSLN"
     gene            complement(13700..14455)
                     /gene="rnc"
                     /locus_tag="PK839_08105"
     CDS             complement(13700..14455)
                     /gene="rnc"
                     /locus_tag="PK839_08105"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HOG72734.1"
                     /translation="MRKFLPKAFWRLRVPAADHEIYRSIEQVLGFSPQNLELYKLALV
                     HRSASLVIAHGKKVDNERLEYLGDAILDAIVADYLFTRFPSQNEGFLTQTRAKIVSRA
                     SLNQLAIGIGLPNIVVLGNGASFNQKNIFGNALEAIIGAMFLDMGFKETSRLVVSNIL
                     ERQVDLEAIQHVEIDFKSRLLELAQKQKNSAEFLTKIESLPESAIPHFTSVLRWDGKE
                     IAHGHGTSKKEAEQNAAMQAMETITEGADKPTV"
     gene            complement(14465..15712)
                     /gene="fabF"
                     /locus_tag="PK839_08110"
     CDS             complement(14465..15712)
                     /gene="fabF"
                     /locus_tag="PK839_08110"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019151097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HOG72735.1"
                     /translation="MEMKRVVVTGLGTINPIGNSVPEYWANLEKGVSGSELITSFDAS
                     KFKTKFACQVKNFDPNAYFDRKELKKIDPYTQFALVAVEEAMKDSGLNTEAIDLDMAG
                     VVWASGIGGMQTFTEELRGYFAGDGTPRFSPYFIPRMISDIAAGYISIKYGFRGPNFS
                     TVSACASSTNGIISALDIIRTGKANVIIAGGSEASVNEVGVGGFNSMQAMSTNNDEYK
                     TASRPFDVTRDGFVIGEGGGCIILEEYEHAKARGAKIYCELKGGGMSADAYHLTAPHP
                     EGLGARKVMENALKDADLTIQDIDYINVHGTATPLGDIAEAKAIISVFGDYAFKLNIS
                     STKSMTGHLLGAAGAIEAIAAIFAICTDTIPPTINFKSPDPEIDSRLNITPNSAQKRS
                     VKAALSNTFGFGGHNASIIFQKI"
     gene            complement(15762..15998)
                     /locus_tag="PK839_08115"
     CDS             complement(15762..15998)
                     /locus_tag="PK839_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOG72736.1"
                     /translation="MSDIATRVKSIIVDKLGVDENEVTPEASFTNDLGADSLDTVELI
                     MEFEKEFNLSIPDDEAEKIGTVGDAISYIEGHSK"
     gene            16179..>16687
                     /locus_tag="PK839_08120"
     CDS             16179..>16687
                     /locus_tag="PK839_08120"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005864028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HOG72737.1"
                     /translation="MSNIAIFASGSGTNAENIIRYFCNHPLIRVTLVATNNPNAYVIG
                     RAKMLAVPVVVFSLEELRNGVLLNLLKLNSIDLIVLAGFIKLVPEQVINAFPGRIVNI
                     HPALLPAYGGKGMYGNKVHEAVLANGERESGITIHYVNNRYDEGDIIFQAKCEVNPSD
                     TPDTLAQKV"
BASE COUNT         4654 a         3895 c         3729 g         4409 t
ORIGIN      
        1 aacctttatc ctttattttc cctccttccc tctttcagaa agatttgcta aaaattgtaa
       61 atttgaggga attttaaata gttaaattga tactaaatgc tttttagcga tatagttgga
      121 caggaaaagg taaaagaacg gctgattcag ctggtaaggg agaaccgtgt gccccatgcc
      181 aacctttttg taggcccacc cggtgcagga aaactccccc tggcagttgc ctttgcacag
      241 tatatcatgt gtgaaaaccg tggcgagagc gattcgtgcg gagtttgccc ctcatgcagg
      301 aaagtagcca agttggttca ccccgacctg catttcgttt tccccaccaa gacaaaggaa
      361 aaatcggatg agggtgccga tgaaaattcc gaaaaacctg acgataccat ttcaacatgg
      421 cgctcggcta ttatcgccaa tccctacttt ggcgagaacg actggtacga aatgatcgga
      481 atggagaata agcagggaat tataagcacc gccactgcca gtgaaataat cagaaaacta
      541 ggcttgaaaa gctttgaaaa cgagtttaag gttataataa tatggctgcc cgaacgtatg
      601 aataatcagg cagctaataa gctactgaaa ctgattgaag aaccgcccga aaagactatc
      661 tttttgctgg tgagcgaaaa ccccacccat atcctaaaaa ccatactttc aagaacccag
      721 caggtgatgg tacctcccat taatcgtgaa agcgtagccc ttgccctgca tgacaagtat
      781 aacctttctc ctgaattggc tcacgacatc agccggatag ccaatggaaa tttttatgag
      841 gccatcaggt tgaaggacca ggaatcaaac tactttttca ccagctaccg gaagctaatg
      901 cgactttgct actccaaaaa cgtgctcgga ctcctcgact gggttgatga aacagcttcg
      961 ctgggcaggg aggacttaaa gcacctgctt gtttactccc ttaggctaac ccgcgaaacc
     1021 ctcgtgctca acatgggagt tgaggaggtt tcatacctga tgggggaaga ggaagcattt
     1081 tgtcacgatt tctcaccatt cattacaaca aataatgtct ttagcattac tgacgagctg
     1141 gttactgcct tcgagtgtat cacccaaaat ggtaacccaa cggtagtttt aacccacatg
     1201 accatgagga tggttaagct aatccccaat aaaaaagcgg cctagaaggt tcataatttt
     1261 ttgctaaatt tgacgacttt taattggcta acctgacgat ggaaaaaaat atagggtcga
     1321 ggggcggggt tataaccgtg aacgaagctt ccgagcattc ggtttgcagc tcaaagggtt
     1381 gcgccaagct aaaagttcac gactggatgg ccgaccttcc catgccgctc cagcaatcgg
     1441 atattattga agttcaattt aaaaataccc gaaagggttt ttaccgaaat gccaaccagc
     1501 tcgttcttcg gcaaggtgat attgtggctg tggaggctac ccctggccat gatataggcg
     1561 tggttactct taccggctgg ctggtagagc gccaaatgaa acgagtagga gttgacccta
     1621 aaactaccga gttcaaaaag gtttaccgta aggcaaaaca ggccgatatt gagaaatgga
     1681 tagaagctat tgcccttgaa aatagcacca tgattagggc aaggaaaatt gccgaggaac
     1741 aaaggctgaa catgaaaatt ggcgatgtag agtaccaggg tgataaaaca aaagccattt
     1801 tctactacat tgctgacgaa cgggttgatt tccgggagct aattaaaata ctggccgagg
     1861 aattcagggt aaaaattgag atgcgacaaa ttggtgcccg tcaggaggca gggcgtattg
     1921 gcgggatagg cccatgcggt cgggagctgt gttgctcatc gtggatctca cactttgcct
     1981 cggttagcac caattccgct aaggttcagg agctgtcatt aaatcctcaa aaacttgctg
     2041 gtcagtgcgg aaaacttaaa tgctgcctta actacgagct cgacagctac atggatgccc
     2101 gcaaggactt ccccgatacc agtattgctc ttgaaaccgc cgatgccact tactaccact
     2161 tcaaaaccga tatcttcaga cggagtatgt ggtacggcag cgagccttct tcgtcggtta
     2221 accttatcga agtctctgtt gatcgcgtta acgaaattat cgccctaaac agaaagggaa
     2281 ttaaagccga caagttgttg gaaaatacta catccgacca gaataactcc cggccaaacg
     2341 gcttcaccga tggtttactc gacgagggta gcctgacccg atttgataaa aaggggggaa
     2401 agaccgcaaa aaggaaaaaa agcagaaatc gcaaccgaac caggccaagc aatgagtaaa
     2461 agcctaccaa ttgctttttt ggcagctacg cttacattgg cttgctgcaa cagggatgcc
     2521 gtatttgatt catacgtagc tattccccgt gaaacttgga gcgccgatag cttggctagc
     2581 ttcaacattg ctattcccga cacttcccaa tcctacgaca tcatgattaa tctacgcaac
     2641 tccaccgact acccgtttca aaaccttttt cttttcatca gcatttactc ccccaacggt
     2701 ggctttgtcc gtgatacctt cgaatgcttc ctggccgacg ataatggtcg ctggacgggt
     2761 aggggtagcg ggagaattca cgacaaccgg ttcatttacc gcgagggtgt aaagttttat
     2821 caacctggca gctacagatt tgaccttcag caggccatgc gagttgaaga cctcagggga
     2881 gttgttgatg ttggaataag aattgaacaa tcccaacaaa actttaaata actagggcac
     2941 aagccactca gcccatatca cgtaaaaatc ataactctct gcaaggtggg taagaataag
     3001 cttaagcgat ttgccgagaa cgaaacattc tccaacctct ttcagccccc ccttgaggag
     3061 gtgcttaacg acaaattcga gctaaaaggg aaatggggga acagctactt taaaaatatc
     3121 aaccctttgg ttctggagct gggatgcggc aaaggggagt acaccgttag cctggccggt
     3181 atgaacccac agaaaaattt tatcggtatc gacataaaag gggccagaat atggcgcggc
     3241 gcaaaaaccg cacttgaaaa caagctgaac aacgtggcgt ttgttcgaac ccgcattgag
     3301 catatatgct caatttttgc tcaaaatgag gtggatgagc tgtggattac ctttcccgac
     3361 cctcaggaac gagagattaa ggccaaaaaa cgcctaacgt cgagccgttt tttgaacctc
     3421 tattcaggat ttttaaaacc aaatgccatt gttcacctaa aaacggataa ccggcagctg
     3481 ttcgactaca ccaaggcgct ggtggaacaa aataaattta atgttttaat ctgtactacc
     3541 gacctctatg ccaattttac caacgatcct gttcttggta taaaaacatt ttacgagcag
     3601 caatttctag gtgagggact tcccataacc tacctccgtt ttgtccttaa ccatcccgga
     3661 ccctaccatg aaccaatccg gtaacgataa tacaggtttc ttcgaaaagg tttacgaggt
     3721 aacccgtatg ataccgtatg gcaggataac cacatatggt gccattgcac gctaccttgg
     3781 ttctgctcaa tcggccagaa tggtagggtg ggcattaaac tcctgccact cccaacccga
     3841 ttgggttcct gcccatcgtg tggtcaacag gaatggccag ctaaccggaa aagtccattt
     3901 ccctggttca aacaccatgc agcagctgct cgaatcggag ggtattaggg ttgaggatga
     3961 caccgttttg gagttcaaaa agttcttttg gaatcccgct accgaacttt aacctttaaa
     4021 taacgtttta taaccatgga catcccaaat tttattctgg gaggcatgac aatgtcctgt
     4081 aaaataacct acttttgtgc ttttctaagt ataacaaaat caacataatg aatttgacca
     4141 ccgatcaggt tttggaggta ctcaaccaag tagtacatcc cgaaagcggg cagagcatag
     4201 tgagcatggg catggttaag gatttgacag ccagcgaccg gcgcatttca tttaccctga
     4261 agctcacaaa gaagaacgat ccctttgcct catcgctcaa aaagacagcc caaagactgc
     4321 tgaccgaaag ggttggacaa caggtagaaa tagacataca ggttgttgcc gacatccctg
     4381 aaagaatatc cccactaaaa accaaagcct cggttagccg ggtaaaaaat attgtggcta
     4441 ttgcctcggg aaaagggggt gtgggcaaat caaccatagc agccaacctg gccgtttcgg
     4501 ttgccaagtc aggcgcccgt gttggactta tagatgccga tatatacgga ccctccattc
     4561 ctaaaatgtt caacgtagaa aacgttcaac ccgaagttat gaaggaggat gagatggaac
     4621 tgattgttcc cgttgagcgt tacggcgtga agcttctctc aattggtttt ttcgttgacc
     4681 ccaacaatgc tcttatttgg cggggaccca tggccaccag cgcactaaag cagctaatcc
     4741 accagggagc ctggggtgag ctagactacc tgttcattga catgccaccc ggcaccagcg
     4801 acattcacct gaccctggtt caggaagttg cggttaccgg tgccgttatt gttagcaccc
     4861 cccaggacgt tgctttggct gacgctgtta aggggataag catgtttgga agtcagggga
     4921 taaatgtccc agtgcttggg ctggtggaga acatggcatg gttcactccc gaagaactac
     4981 ccgagaatag gtactacatt tttggaaagg atggttgcaa aaagctggct gagcgcatga
     5041 aactaccgtt gctagggcaa atccccctgg tgcaaagcat tcgtgagggt ggcgatttcg
     5101 gtgaacccgt tgccctgcac tcatcggtgg tggccaaagc atttgatgag cttgccacaa
     5161 atcttattag acaggtcgaa attcgcaaca gggaacttcc tcctactacc attgtaaacg
     5221 ttaaacacta ataaattatc catttcaaaa tttttaaacc atgactagca aggaggaaat
     5281 tgtaacaaaa gtagctgcag ccattgatgt tattcgcccg tttcttcaga atgatggcgg
     5341 tgacatctca ctggtagaag taaccgatga caatacggtt agggtgaagc tgcatggcgc
     5401 atgcggtagc tgcccctaca gcctgatgac tttgaagaat ggagtagaac aagccatcat
     5461 gcgcgatgtt cccgaaataa gaggggttga agcagttaac ctaaacatac cctaacaact
     5521 ttttttcaag tatcctaacc atttagaatt ctagaacttc aggtaacgga aaactccctt
     5581 tttaaaggga gtttttttgt agtataaaat atgtggttgg caatcataaa attataatcc
     5641 catcaacttt ttccccgggt ctgtcaaaaa tcaaacgcaa acccttgcaa aagcaaagtt
     5701 ccccatcgac atttacacca aattatcatt aatactttca acctatgaga tacgtactac
     5761 tttttaccct gatgctggtt gttgcctcag ttgctgcgca ggaaccagta acgcttaaat
     5821 ttacgcgtcc atcaaaggtg caggcttcag catcaaaaat caagctgaaa gtccaaggta
     5881 acgaatacgt tcttaagaat ggagcaagca tatccatcaa cgttaccccg gagtacggaa
     5941 agtcgttgct gattaatgcc ggagaccaaa ctgcctacta cttgaaacct aaacctgggg
     6001 aaacctacga gtttgaggtt ggttttaaat ttgcaggccc ttacatactg ctgctgagcg
     6061 gggaagaggc caaaccaggc gaggctgtcc aggtaaccga caccactgcc aaacccgagg
     6121 ggtgggaaac caacctaaag gttgaccggg ataacatggg agctggaatt acccttcaaa
     6181 aaaccgacca gtccgaggca ataaggcagg agtggctggc tcgcgggggt aaaattaaat
     6241 accaatcggt tgttttcaac ggaacctatt ttggtatgga catggacatg gagggcgttg
     6301 acgcaaacag gatagacggg tacggaggcg gcatttcgtt ctaccagaac aggattaacc
     6361 ttaaaattcc tgcctacaaa accggagctt catcatggaa ctcctttaac atcggttttg
     6421 gatacgacat gctggtgtac gcatacaaat ttgacatgaa gatggacccc ataacaacta
     6481 aaatggatgt ggttactatt accatgccat tctcggccaa tattggatgg acactgggtt
     6541 tgggaaagtt tattgatgag gggaactgga agggagttgc ccttacccta aggtaccgtc
     6601 cttccattat gtttaacttt accaactcaa ccgttaagat gacctcaacc gatccccaca
     6661 tctccggctc tacttccacc agcaccgaca gcaatgtgca gtttaacgct ggtggtgtgg
     6721 gcttcgacgt tgatttctca aactttagct ccaccatgga gaagctcgcc ccgaaaccca
     6781 agcttaaact ttcgttcttc gtcatgcccc ccgttaagga catgcccctg tttatcagct
     6841 ttgggctggg tataactaca tactcccgct agtaatttat aaatgaaaac acacaccaaa
     6901 attatcagta tgctgattac aagctaaagg tcataccccg ataatctaaa agtcttgggc
     6961 caaacccgac atcagaacta taattacaat agaaataaac agtggagtcc taaaaccact
     7021 ataaccaaga ggggcgttac cgaaaagcca aacgagtagg aaaaaattca acttaactct
     7081 aattttatga gaacactagg aaacatttta tggcattttc cttttttggg gtttttaacc
     7141 gcacttggaa cctttttaat aggcggtttg ctggttataa ctgtaattgg ctcaccactt
     7201 ggtctggggc taattcagct ttccaagttc ttgcttgccc cattctccaa cgccatggtt
     7261 gataaaaccg accttcaggc cgatcaaaat aaggcatgga agacttacgg gattattgtc
     7321 cggattcttt acttcccgat tgggctattt ctggttgttg taaacatttt tcaaattgcc
     7381 ggcctattta tttcaatcgt tgggatacca gtggctctgg ttttggcaaa atcaattggt
     7441 acttacttca acccggtaaa caaggtttgc gtttcaaaag cagttgccga tgagcttagc
     7501 tcaagaaagg ctaaggcaga ggttgataag gcttttgcaa caaagtaaag aatgtagcat
     7561 aacattccat ccaacaaatt taaaaaggca ttttaaatgc cctatcctcc ttggcttgtt
     7621 acagcaacca gggaggtttc ttttttgtaa aagcatagtg aaaataaagg ccgttttgat
     7681 aaaagtttac tataaataca tttatattta cagaataaag cccaaaaata tggtcaaaaa
     7741 aatcctcatg ctgctacttg ctaccctaat gtgtccggca ctatacgcca tacccgacac
     7801 gtcaagggtt gcgttgctgg ttatagatat tcaggatttc tactttccgg gtggcaaatc
     7861 ggagctggta aacccgttgc ctgcggctaa gaatgcagcg ctgctggttg aatacttccg
     7921 atcgcaccag ctgccggtta tatacgtaaa acaccagtcg gcagaacagg aagaaattaa
     7981 cgagatggta aagcctctgg ccgatgagcc gattttcacc aaaacggagg tgaactgctt
     8041 tgtggggaca caactttacg actaccttgt gggccagcaa atttcaaagc tggttatctg
     8101 tggtatgcaa acccacctct gtgttgaggc agcagttagg gctgcttcgg actatgggtt
     8161 caaggtaacc cttattcacg atgcgtgtgc caccagggat gttaaatggg gcaatactac
     8221 cattccggcc aacatggttc acctgtcaac cctttcaacc cttcaaaact acgccagaat
     8281 acttagtacc gaggaatgcc tcaaggccaa cccttaaaga tttaaccact taaaacgcag
     8341 gactggcaaa agctttaaca tcgcctccgg ttatgtcctt gtcgcatagt atgataagcc
     8401 gttcgacaac attccgaagt tcccttacgt tcccggtcca gggtagccgt ttcaactcgt
     8461 caacagcatc gggtctaaac accttaggcg gtatgccata ctcggagcaa attagctggt
     8521 tgaaatgttc tgcaagcaat ggaatatcct caaccctttc gcttagcggg ggaacatgaa
     8581 tgatgatgac gcttaaacgg tggtaaaggt cttcgcgaaa gttaccattt tgaatctcgt
     8641 gggtgaggtt tttattggtt gccgcaataa ccctcacgtt aacgggaata tccttatcgc
     8701 tccccacacg gctaatcttg ttctcctgaa gagctctgag cacttttgat tgagcgctta
     8761 gactcatgtc gcctatttca tcgaggaaaa gcgttccgcc atcggcctgt tcaaatttac
     8821 ccttgcgctg cttgattgcc gatgtaaacg ctcccttctc gtgtccaaaa agttcgctct
     8881 cgataagctc cgatggaatg gcagcacagt tcacctccac aaaaggcatg tcggagcggt
     8941 tgctcttttc gtgaagccat cgggctacca gctccttccc tgttccgttt ggtccggtga
     9001 tgagcaccct tgcctcggtt ggggcaaccc tgtctatcat ctccttgacc ttaacgatag
     9061 ccggcgactc gccaactatg tcgtaggcct tattcacctt gcgcttaagg attttggcct
     9121 cctgaataag ggtagtttta tcggttgcgt tgcgtatggt gataagcaaa cggttcaggt
     9181 ccaacggttt ctcaataaag tcgtatgccc ccttctttat agactccaca gcagtgtcaa
     9241 tgttcccatg tccggatatc ataaccaccg gtacatcggc tgcttgttcc tgaagctttt
     9301 caaggacctc aatgccatcc atctggggca ttttaatgtc gcagagaaca atatcgtagc
     9361 atccggagcc aaacatctca attccggttg ggccatcttc agccaaatca accgaatggc
     9421 tttcgaattc aagtatctcc ttcagggtgc ttcggatagc cttctcgtcg tcaatgacaa
     9481 ggattttggc catagctcaa tttttggtaa atataccaaa taatgcttaa tgaacgtgat
     9541 taaaaagcat ggctgctttg cagctcggcc ataaatccct ccttaagggc gaatgatgac
     9601 tggaacaact tgttaattcc tattttttgg atgatgaggt taataaatac tgaggccgga
     9661 acaatcaggt caacccgcat ggggtccagt ccctttagcg ccatgcgctg atcgtgggtg
     9721 gaggtgataa aaaaattgtg cagctcgaag taccgctcaa ggggtatttc ggcccagggt
     9781 tcgctaccag gagcaatgct tcccgagtgc tccatgatgc tacgaattga atcgaaagag
     9841 ccggagcttc ccaccaatcg gacaggaaca tacctacgcg cagcatcaaa aagagtttga
     9901 acttccacat taataaactc ttccagagcc ctcagctgct tctgtgttat aggatcggaa
     9961 agttgaaact gctgcatcaa ccttgaaaca cccaggttaa agctgtgaag ccaaaatggc
    10021 tcgctggtat tcccaagtat tatctcatta cttccaccac caatatccag aatcaagcta
    10081 caggtgttac ccaaatctaa tgcctccctg acccccttgt acaccagcgt tgcctcgcga
    10141 ttaccgtcaa ttatttcaac attgagccca aaactggtgc gaatatcctc aacaaagttt
    10201 gtcccgtttg aggcatcccg ggtagccgaa gtggcaaatg caaagaactt accagcaccc
    10261 aagcttttcg ctttagccat gtgggtatta attgcatcca ggccacgctg ccatgcctcc
    10321 ggagtaataa tcttcctgtt taaaccgccc tcgcccagcc taaccgggag cttttcctcg
    10381 taaataatct cggggcgttc ttcctggtta aacctggcta ccaacaggtt gaaggtgttg
    10441 gttcccatgt caataattgc cacattcata acctaaacat ctaatctaaa acactccact
    10501 ccttaaaaaa ctgcgtaaga aacagctcca taaactgatg cctttcaaca gcaatttgcc
    10561 ttgccgtttg ggtgttgagc ctatctttca aaagaagaag cttttcgtaa aagtgattga
    10621 tggtgcttcc gtgggcatgt tggtactcat caaacgagtg gtgttgctgg ggcttcaaat
    10681 caggaatgta catgggccgt tgcctggctc cgccgtaggc aaatgccctt gcaactccaa
    10741 tggcgccgat ggcatcgagc cggtcggcat cctgaacaac ctttgcctca atagtggaag
    10801 gagtagtatc aacaccggca cccttaaatg atacctgccc aatgataagt gcaacacgcc
    10861 gggcttcatc atcgctatat ccggattcgt gcagtattct gagcgcaaca gggcaaccct
    10921 cggtgtttct gttcagcttc caatcgtcaa catcgtgtag caaagcggcc agctcaaccg
    10981 tgaagggatt ggcattttcc ctttctgata tgaacaacgc cagcttaacc accctgagaa
    11041 tgtgccacca gtcgtgacct gttgaatcgc cccggcaggc aaatttaaca gattggtaaa
    11101 cctgttgtaa atcgttatcc attggggtaa tacaagtttg taaaaaaagc gcttcaaaat
    11161 gcgaagagcg aaaatccgat ggattattcg ccctcagctc aacaagctta ttacctgctg
    11221 ctagcttcta tacctaatcc actttatcat ctccttccac gagtacttct tgccgtacat
    11281 cagaattccc accctgtaaa tttttgcagc taaccataca gtaacaacaa aagttccgat
    11341 tagtagcgct gccgaaagga taagctgcca tacaggaacg ccaaatggaa gccgggttag
    11401 catcactatg ggggaggtga aaggtatcat cgagaaccag aaggcaaggg aaccgtcggg
    11461 cgatttaatg ccaacaatca taacaaatat cgccagtata aggggaatgg ttatgggcat
    11521 aaccagctgc tgggtgtcgg aaacattttc tacagccgaa ccaacagcag cgtacatggc
    11581 ggcatagagc aggtacccca tcaggaaaaa gaatataaaa ccgccgatta tcaatccata
    11641 gttctgattt ttcagggctt gtagaaagtt atcggtattt gtttcagaga ttgctgctgt
    11701 tacatcggct gactgagctc cttggttggc catgatggac tggttggcaa tttgaacctg
    11761 ctccatattg gccttgcctg atttttccga tatgaggtta aggccaactc cggccaaacc
    11821 ggcagtgagt acaatccaaa ttgcaatctg cagcaacccc accgctgcta ttccaaatat
    11881 tttgcccatc ataagctgta cgggctttac agacgaaacg agtagctcaa caacgcggtt
    11941 attcttctcc tcaattaccc cctgcataac ctggttgccc atcagcatca ccatcatgta
    12001 catcaggaaa cttaaaatat aagctattcc catggttatc agggtattgc tctccttttc
    12061 ctcgccatcc tttgaaattt tgatggagcg gacgttaacc ttagttttta cgtccttgag
    12121 tattttatca aggtcctgaa tgttgtaggt catcaactta cgggtttcaa tttccttctc
    12181 catggagttc tcgatatgcg aaatcacctc aatggaaggt tgtttgggcg agtagatggt
    12241 aagggctggg gcaatgctac ccggaagtga atcaattgta agcagggcgt agtagcctga
    12301 ctcctctagt gaatttttta ccttttcggc cggcgtatcg gttatggtag taattttaat
    12361 gtattcggtt tggggaatag cacttccaaa gtgctgtgta ttgtcaataa cggcaatatt
    12421 gcgcacttca gtgtcctcca tggtggcaaa ataggtagga agaattacca tggcggcaaa
    12481 aaatagcggc gaaagcaggg tggtaaaaat gaatgatttt ttccgcaccc gggtcataaa
    12541 ctcccgctgg agtataagga acatttgttt cataattccg gaatttttta taaaatacta
    12601 gttaagcgat aaaccatgag ccttgttgta ctcctgcaca acccgaataa agatatcgtg
    12661 catgctgggt actacgggat taaacgaaag cacctcaaca cttgaaatca ggctttgaag
    12721 cagcttgttg ctatccgaag ccgattgcag ccttacctgc acccttggaa tagccttctc
    12781 ggtgttaacg ttaaaaatgt caaatgtacc gttcaggttt tgcctcaggg tttcgaggtt
    12841 tcccttaaag gcaacttcaa atacgttgct gccatagtta gcccgcacct ggtcaatttg
    12901 cccgctaagg atattttttg atttattgat gagggtaatg tgatcgcaaa gctgctcaac
    12961 cgattccatg ttatgggtgg aaaagataat ggtggtgcca tttttcttca actccagtat
    13021 ctcatcggta atcagctggg tgtttatagg gtcgaaaccg ctgaatggct cgtcgaatat
    13081 gagcagctcg ggtttgtgga taacggttac aatgaactgc accttctggg ccataccctt
    13141 tgagagctcc tcaatttttt tatcccacca ggattgaatt tcaaattttt caaaccagta
    13201 cttcaggctt ttagttgcat cttgccggct taaccccttg agctgggaaa agaatagggc
    13261 ttgctctcca accttcattt ttttataaag accacgctct tcgggcaaat atcctatccg
    13321 gtagatgtcg ttgggttcaa ttttgcggcc atcgaacata acttctccct tgtcggggcc
    13381 ggtaatctgg ttaattattc tgataagtgt tgtttttcca gcgccgtttg ggccaaggag
    13441 tccatagata gatccctttg gaatggcaat gctgatatcg tctagcgcca ggtggtttga
    13501 aaatcgcttc tccaccccat gagtctctaa aagattcata aatctgatgg gtttaagttc
    13561 acctatgaat aaaattatgc aaatgtagca aaagcaacta ataatcctta caataaatac
    13621 ttaatcagca cctttgaaga atgcgctctt gcatgcagcg cagaggtatt agtagaagga
    13681 agaggtaacg aaaatgagcc taaactgtgg gtttgtcagc cccctctgta atagtctcca
    13741 ttgcctgcat agcggcattc tgctcggcct cttttttaga agtaccatgg ccatgggcta
    13801 tttccttgcc atcccagcga agaacagagg taaaatgggg gattgccgac tcgggcagtg
    13861 actctatttt agttaaaaat tcggcagaat ttttttgctt ctgggccagc tccagcagcc
    13921 tgcttttaaa atcaatctca acatgctgaa tggcttcaag gtcaacctga cgttcgagaa
    13981 tattactaac cactaaccta ctggtttcct tgaatcccat gtcgagaaac atggcgccaa
    14041 taattgcctc aagggcatta ccaaaaatat ttttttggtt gaacgaggca ccgtttccca
    14101 gcactacgat atttggtaaa ccaattccta tggctagctg gttaaggcta gctcggctaa
    14161 caattttggc ccgtgtttga gtaagaaaac cctcattttg gctggggaat cgggtaaaaa
    14221 ggtaatcggc tacgatagca tcgagaatgg cgtcgccaag atattcgagc cgttcgttat
    14281 caaccttctt tccatgagca ataactaacg aggcagacct gtgaaccaag gccaacttat
    14341 agagttctag gttttgaggt gaaaatccca gtacctgctc aatactacgg tagatttcat
    14401 ggtctgcagc aggaactctc agacgccaaa atgcttttgg aaggaatttc cgcaccggta
    14461 gctattaaat cttctgaaaa attatagaag cattgtgccc accgaatccg aatgtattgc
    14521 taagcgctgc cttaacgctt ctcttctggg cgctgttggg tgtaatgttt aggcggctgt
    14581 caatttccgg atcgggtgat ttaaagttaa tagtgggtgg aatggtatcg gtgcagatag
    14641 cgaaaatagc agcaatagcc tcaatggcac cggcagcccc aagtaggtga ccggtcatgg
    14701 attttgtgga gctgatattg agcttgaagg cataatcgcc gaataccgaa attatagcct
    14761 ttgcctcggc aatatcgcca agaggtgtgg cggtgccgtg tacgttgatg tagtcaatat
    14821 cctgaatagt aaggtctgca tccttcagag cattttccat cacctttcgg gcgccaagtc
    14881 cttcggggtg cggagctgtg agatggtaag catcggccga cataccaccg cccttcaact
    14941 cgcaataaat ttttgcacct ctggcctttg cgtgctcgta ctcctcaaga atgatgcaac
    15001 caccaccctc accaattaca aaaccatcgc gagtaacgtc gaatgggcga gatgctgtct
    15061 tgtactcatc gttgttggta gacatagcct gcatggagtt aaagccacct acgccaactt
    15121 cgttgacgga tgcttccgac ccaccagcaa tgataacatt tgctttcccg gtccgaatta
    15181 tgtcgagtgc cgaaatgatc ccgttggttg aggatgcgca ggctgaaacg gtggaaaagt
    15241 tagggccgcg gaatccatac tttattgaaa tgtatccggc ggcaatgtcc gaaatcatcc
    15301 tgggaataaa gtaagggcta aatcggggtg ttccatcacc cgcgaaatag cctcttagct
    15361 cttcggtgaa agtttgcatt ccaccaatcc ccgatgccca aacaactccc gccatatcaa
    15421 gatcaatggc ttcagtattt agcccagaat ccttcatggc ctcctcaact gccaccagcg
    15481 caaactgagt gtaagggtct atctttttta gctccttacg gtcaaaatag gcattgggat
    15541 cgaaattctt tacctgacag gcaaatttgg ttttaaactt tgaggcgtcg aaactggtaa
    15601 ttagctcgct tccgctcaca cccttttcga ggttagccca atattccggc acactgttgc
    15661 caataggatt tattgttccc aggccggtaa caacaacacg cttcatttcc ataaattaga
    15721 aatgtttagt ttaaaccaat tgcctataag gattttacaa attacttgct gtggccttca
    15781 atgtagctaa ttgcatcgcc aactgtgcca atcttttcag cctcgtcatc aggaatcgag
    15841 aggttaaact ccttttcgaa ctccatgata agctcaacgg tgtcaagaga atcggcacca
    15901 agatcattgg tgaagctagc ctcgggggta acttcattct cgtcaactcc cagcttgtca
    15961 acaataatcg atttaactct ggttgcaata tccgacatag ctttaaaatt tagttagtaa
    16021 ataaagtttt catttgcaaa gtaaaaaaac ttttcacctg atacaaaacc agatgaacat
    16081 tttccagccg caaaagtaat ttttttgttt ttacatttgc caaatccttt taaaaaaaat
    16141 ttgacaatcg tttaatttta taacgcattg tgttggttat gagtaatata gcaatattcg
    16201 catcgggatc gggcaccaat gccgaaaaca tcatccgcta cttctgcaat catccactta
    16261 ttagggttac gttagtggca accaacaacc ctaacgctta cgtaataggg cgcgcaaaga
    16321 tgttggcggt tccggttgtg gtgttttccc tggaggagct acgtaacgga gttctgctta
    16381 atcttctgaa attaaacagt atagacttaa tagtgctggc tggttttatt aagcttgttc
    16441 cggaacaggt aatcaacgca tttccaggac gaattgtgaa tattcatccc gcactgctac
    16501 cggcatacgg tggtaaaggt atgtatggga acaaagtcca tgaggcagtt ctagcaaacg
    16561 gtgaaaggga aagtggtatt accatacact acgtgaataa ccggtatgac gagggtgata
    16621 ttatttttca ggctaaatgc gaggtaaacc catccgatac tcccgatacc cttgcccaaa
    16681 aagtgca
//
LOCUS       DAOSHM010000051        16177 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_2950_length_16177_cov_18.956209, whole genome shotgun
            sequence.
ACCESSION   DAOSHM010000051 DAOSHM010000000
VERSION     DAOSHM010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 16177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16177
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2950_length_16177_cov_18.956209"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            302..1153
                     /locus_tag="PK839_08125"
     CDS             302..1153
                     /locus_tag="PK839_08125"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72738.1"
                     /translation="MLGRFLSPDSYVQAPDFTQSFNRYSYCLNNPLIYTDSDGEFFLG
                     TIFTFVGDFLRTTLFHGGLSFNKNVRREAWKDFDPSASWSATNKAWKIDIGGFKTDPH
                     RTIVGRGLQFLSRWTWELPQTFLGKGYSHIRNMTGNVDDVSYYGGATLVNKNNNSRER
                     WGLTLGPYINSKNVRADPYRDPLFRHEYGHTLQSRLVGPLYLTHVGLPSLFGSGLDNL
                     GLSDHNREWYETQANRMAERYFSNHDPGALTALPWDDNRYPREYNPNWYWTFAHPPLP
                     FMWWLFF"
     gene            1172..1567
                     /locus_tag="PK839_08130"
     CDS             1172..1567
                     /locus_tag="PK839_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72739.1"
                     /translation="MKMNILIILSIMLCGASCEKLVDHVYSIKIQNNTNDTLLFYESY
                     NYPDSSIVQNKPILTRVYPKDYSYLDSKKDWDEVLVSPKDTISIFILSKGTVDKYSWE
                     AIRADYKILKRYDLSIQDLENNQWTITYP"
     gene            complement(1855..2799)
                     /locus_tag="PK839_08135"
     CDS             complement(1855..2799)
                     /locus_tag="PK839_08135"
                     /inference="COORDINATES: protein motif:HMM:NF025616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HOG72740.1"
                     /translation="MKTTQYLIAFGLQLLLLGCGDKANREEVIVTNAEEQAYFAAETS
                     PAESKSVIQESSSDFSGSSNVSAKIIRHGNINIEVKDYLVARQQIDSLANLYEAAVIS
                     ESFQENETQRGNSISIRVPAEKFDLLLGELAKIAHRVDYQQISTEDVTTEYIDVNTRL
                     QNKMKVERNYQRLLSQATSVDDILKIENQLGAVRAEIESLQGRMNYINHQVSKSLITV
                     YVYQKVEYRYRPDEMPSFGERIKQAINAGWKGLVWLVVFIIKIWPIWLAALVVFVLVN
                     RLRVIGRRRKAQKKEQRRLEKKLRKYRNDKPSDTNPQQ"
     gene            complement(2968..3210)
                     /locus_tag="PK839_08140"
     CDS             complement(2968..3210)
                     /locus_tag="PK839_08140"
                     /inference="COORDINATES: protein motif:HMM:NF024672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HOG72741.1"
                     /translation="MEVNFELEGEYIPLDGLLKRCSLASSGAEAHLLVDDGKVKLNGK
                     TESRRRAKVHPGDTVQACGHTIHISASNSNRFDEIS"
     gene            complement(3339..4583)
                     /locus_tag="PK839_08145"
     CDS             complement(3339..4583)
                     /locus_tag="PK839_08145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72742.1"
                     /translation="MKQQIIENINNPENLERLYRENRQEFSESFAEIADGYNSELVGF
                     WKVRLANENTTRSKGLLWVDLSVALAIALITGLLVKLPDFFEGIVDETFYIRNLAIIV
                     FNGIIVYTLWQNRISGWKRLLAYGMTIVALIIYSNLLPHRDSDSINLVFIHLPLLLWC
                     LFGLAFVSFDYRSIPRRVEFIRFNGELAVMTGLILIAGVLFSVVTVGLFSAINMNIEE
                     VFMKNAAIFGGVAAPIISYCLIKIYPSITRKVAPVIARIFSPLVLITLAVYLVSMIFS
                     PKSIMEDRNALMIFNVMLLAVVAIIVFSISELDKSRKKDRNVLVLLLLATLAIVTNLI
                     ALVAISARISHGLTPNRTVVLASNILILINLVLLARDLFLSYFRDKETERVEQTMAGY
                     LNIYFYWTLVVIFILPFAFGFR"
     gene            complement(5063..5353)
                     /locus_tag="PK839_08150"
     CDS             complement(5063..5353)
                     /locus_tag="PK839_08150"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HOG72743.1"
                     /translation="MSNHNTKSLGEAIREFITAMKWDEKINETSIIGDWEKIVGRIVQ
                     MHTTKIKLKDGQLTIQVDSSALRHELHMRKQQLIKRINDFYHASIVSDIVLR"
     gene            complement(5358..6467)
                     /gene="recF"
                     /locus_tag="PK839_08155"
     CDS             complement(5358..6467)
                     /gene="recF"
                     /locus_tag="PK839_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130439.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HOG72744.1"
                     /translation="MHLKHLSAINFKSFRQVELELNPKINCFIGNNGQGKTNLLDAIY
                     YLSMCKSAFNSSDRQVLRHGEQFFVIEGSYQRVSSNEDIYCGFKLDEGKIFRRNGKTY
                     DRLADHIGFIPIVMVSPTDSALIDESGEERRKYMNGVISQYDRVYLDEVMRYNRLQAQ
                     RNSILKNHPHVSFSYDLLEAIDDQLVRYAQTIHAKRSSFVEQLIPIFMDYYNRVSGGE
                     ERVSIAYRSHLNDGDFKTMLRNNIQRDRTLQFTSIGIHRDDMVLSIDGYPIRREGSQG
                     QQKTFLIALKLAQFEFLSRVSGIKPLLLLDDIFDKLDMDRVGFLINQVAHDGFGQIFI
                     TDTSKNRLDMILNRLNSGFRLFSIENSEVSIIAST"
     gene            6593..7285
                     /locus_tag="PK839_08160"
     CDS             6593..7285
                     /locus_tag="PK839_08160"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG72745.1"
                     /translation="MAKNNKNAQPDTVESIDNALSQTERFIEQNQKSLTIIIVAILVI
                     ISLYFGYKRFYLVPKEDEARSQIFQAEYYFEKDSFKLALNGNENDLGFVDIADNYSMT
                     KTGNLACYYAGICYRQLGDFNKAIEYLKKFDAGDIMVTPIAYGAIGDCYVELKQLDEG
                     VKFYIKAAEYSDNDFTSPFFYKKAGLIYEVMNNNGDALKIYQTIKDKYPKSNEARDIE
                     KDIARNKVILQK"
     gene            7397..7900
                     /gene="ribH"
                     /locus_tag="PK839_08165"
     CDS             7397..7900
                     /gene="ribH"
                     /locus_tag="PK839_08165"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HOG72746.1"
                     /translation="MATKLKNLSAYDPAEIPSAKKMKFGIVVSDWNEEVTKALLEGAY
                     QTLIGNGVKEDNILVKHVPGAFELTLGAQFMAEYADLDGVICLGCVIQGETRHFDFIC
                     QSVTQGITQLNMDYNIPFIFGLLTTENLEQALERAGGKHGNKGVEAAITAIKMAHLQQ
                     EMEQLEG"
     gene            8298..8654
                     /locus_tag="PK839_08170"
     CDS             8298..8654
                     /locus_tag="PK839_08170"
                     /inference="COORDINATES: protein motif:HMM:NF021221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HOG72747.1"
                     /translation="MEHINYTSENERTFAMLCHLSALAGCIIPLGHILGPLIVWLIKK
                     DEFPEVDRQGKAALNFQLSVTIYGLIAGVLVILLVGFVLLAILAVFTLVLVIVASVKT
                     ANAEKFEYPLAITFLR"
     gene            8789..9070
                     /locus_tag="PK839_08175"
     CDS             8789..9070
                     /locus_tag="PK839_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HOG72748.1"
                     /translation="MFRTNEYFDGNVKSIAFETPDGRATLGVMAKGEYEFSTSTIEEM
                     TVVSGEMSVLLPGETSWKKYVEFETFIVPKDSRFRVRVDGDTAYRCYYR"
     gene            9149..9529
                     /locus_tag="PK839_08180"
     CDS             9149..9529
                     /locus_tag="PK839_08180"
                     /inference="COORDINATES: protein motif:HMM:NF012354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOG72749.1"
                     /translation="MAGSKGSKYYDVYLDYSIFLKRRGSNELVLSQEQIELLLLVDEL
                     QSITASARQMCISYRKAWELVRQSEQNLGFTIISKSRGGKNGGSSVLTPDGKLLANAY
                     RQLQADFNSSVKRVVKEFFQTINR"
     gene            9535..10488
                     /locus_tag="PK839_08185"
     CDS             9535..10488
                     /locus_tag="PK839_08185"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOG72750.1"
                     /translation="MRKIKFLLLLIAPLVVLQCHSGRSKQKELIVFHAGSLSLPMKAA
                     ADSFMKLHPDVRILLEAAGSMECARKISELHKPCDLFVSSDYKVVEMLIDQGFATWCI
                     PFASNEMVIAYHDKSRLADCINGSSWPSVLLSKEVAFGRSDENSDPCGYRTIMTIRLA
                     EDLYHRPGLADSLLAKDVKHIRPKEVDLIALLEANVIDYLFIYKSVAQQHGLNYLELP
                     AAINLSSKEYESVYSRVSVEVVGSTPDARVALTGEPMVYGLTIPRNAPNFDIAVQFTA
                     FFLSENSGMRILSAMGQTPIIPTSTKTYNNIPQALRAFATP"
     gene            complement(10552..12258)
                     /locus_tag="PK839_08190"
     CDS             complement(10552..12258)
                     /locus_tag="PK839_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylase"
                     /protein_id="HOG72751.1"
                     /translation="MALNPRKILIRDVTLRDGQQSLFATRMTQKQVEQVLPYFKEAGF
                     YAMEVWGGAVPDSIMRYLNEDPWYRLESIKKVIGDSTKLTALSRGRNLFGYNPYPEEV
                     IEGFNRIAVNSGISIMRIFDALNDTNNIRSTVKYVKENGGLADCTVCYTVDPKFSKKD
                     RVKAVINRKKLPGKIFTEAYYLSKAKELEAMGADMITIKDMAGLVTPDRAASIISTLK
                     KEIKVPINFHTHCTPGYGLAATLAAIVNGVDIVDTAIWNFAGGPAAPAFELIQIFCQK
                     LGIETGINLNAAATINTELKSIRTELKSFDTYPLPHEFDITHPNLPLQVDSLFEQAIQ
                     QALSAQYDELTKTCQAIESYFGFPAPDETVKHAEIPGGMYTNMLAQLKQLKLEKLLPR
                     VLEIIPTVRLDAGCPPLVTPTSQIVGAQAVNCIIDENKGLPFYTTKSTQFVNLVKGSY
                     GKTPLPIDPQFRLKITGSAEEVPFNTASYKKQPNPSFPEYGEGVKLAMNEKEELLLEL
                     FPNVTEKYLRDKRVAHFEELKRKKQEEERRRIEEEKQKYNSMTEQERWQRLQTGLISY
                     YF"
     gene            complement(12483..14357)
                     /gene="yidC"
                     /locus_tag="PK839_08195"
     CDS             complement(12483..14357)
                     /gene="yidC"
                     /locus_tag="PK839_08195"
                     /inference="COORDINATES: protein motif:HMM:NF002356.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HOG72752.1"
                     /translation="MDRNTILGLVLIFLIMIGFGYLAAPSKEEREAYVRKADSLARVQ
                     AEYEQKRAEEEKAKAAMLADTIQKKEVQSQVGSFAPALEGERKFISLENDLMRVVVSN
                     RGGRIYSVELKKFKTHAGEPLVLFNGDDNEFGLQFWGENNAVKTNDLFFVPQTSDTAV
                     TASESQPASLTMRLQIADSSYIDYIYTVKPNSYMVDFDIRFNGMTKSIGGQAGSIDLL
                     WNLKLNRLEKGIENERNYTTIAYTFLNNDYEELNPRSQDEKKELTTKLKWVDFKHQFF
                     SSILVANEHFINSDLKMTNLNDSLHVKAMDARLSLPFKNQNDETLKLGFYFGPNHYKT
                     LKEYNQGFEKVVPLGRNIVRWINKYVIILLFNWLSSFLTNYGIIVLIMTIIIKLVLLP
                     LTYKSYLSSAKMRVLKPQIDEISKKYPKKEDAMKKQQAVMALYKKVGVSPMGGCLPVL
                     IQFPILIAMFRFFPASFELRQQSFLWAEDLSSYDSILHLPFTIPWFGDHISLFALLMA
                     ISMVITSKMSNDQMADTNAQMPGMKFMMTWLMPVMMMFWFNNYSAALSYYYLLTNLIS
                     MLQTVLMRRFVDDEALLAKLHENAKKPVTKSKWQMRMEEVAKRQQELQKRQDAVRKKR
                     "
     gene            complement(14435..16048)
                     /locus_tag="PK839_08200"
     CDS             complement(14435..16048)
                     /locus_tag="PK839_08200"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HOG72753.1"
                     /translation="MPHNTKYVFVTGGVASSLGKGIISASLGKLLQSRGFSVTIQKLD
                     PYINVDPGTLNPYEHGECYVTEDGAETDLDLGHYERFLNLPTSQMNNVTTGKIYQTVI
                     SKERHGDYLGKTVQVIPHITDEIKRRIKLLGTKHKLDVVITEIGGTVGDIESLPYIEA
                     VRQLRWELGPTNSAFIHLTLVPYLSASGELKTKPTQHSVKVLLENGIQPDVLVLRTEK
                     PLNDDIRRKVALFCNVDSSAVVESIDVKTIYEVPLLMHKEKLDETIMKKLNLPVQEPP
                     KLDDWNEFINKLKNPKSKVKVGLVGKYVELHDAYKSIVESFIHAGAVNECHVELEMIQ
                     SESITASNVKEKLGHLQGILVAPGFGHRGVEGKIEAIRYARENQVPFFGICLGMQCAV
                     VEFARNVLGLQEANSTEMVRNTPFPVIDLMEEQKGITDMGGTMRLGSYPCQVKKGTKA
                     YMAYHKLETHERHRHRYEFNNQFLPQFEAAGMVAAGINPETNLVEIMEISSHPWFVGV
                     QFHPEYKSTVINPHPLFVEFVKATMNFGG"
BASE COUNT         4151 a         3495 c         3765 g         4766 t
ORIGIN      
        1 ttacaggcaa ataccccatt agagctggta gctataccag ccctggtggg gtttctcttt
       61 tgataaatcg ggaaatattt acctcatttt ttgtaataga tagtatgata agacccagaa
      121 gcatttcatt aatgttttgc cagtgggagt cctcgataca agtatggatg acgaggtaaa
      181 gttttccaag tttgttcttt cagtaaaatt cttttacttt tggatacatg ttctttggca
      241 tttacaagga aaggtaaagg cctaagccgc aatttaattt acccatacag gatgtatccc
      301 cgtgctgggc cgcttcctca gccccgacag ctacgtgcag gccccggact tcacccagag
      361 cttcaaccgc tacagctact gcctgaataa cccgttaatc tatacagatt ccgacgggga
      421 gttcttctta ggtacaatct ttacttttgt tggggatttt cttagaacga ctcttttcca
      481 tggcggtctt tcatttaata aaaacgtaag aagggaggct tggaaggact ttgatccttc
      541 tgctagttgg tctgctacta ataaagcgtg gaaaatagat ataggaggat ttaaaacaga
      601 tccgcatcgc actattgtag gcagaggtct tcagtttctt tcaagatgga catgggaatt
      661 gcctcaaaca tttcttggaa agggatactc gcacattaga aatatgacag gcaatgttga
      721 tgatgtgagt tattacggag gggctacctt ggtcaataaa aataataatt caagggaaag
      781 atggggactt acattaggcc cttatataaa ttctaagaat gttagggcag acccatatag
      841 agatcctttg tttcgtcatg aatatggcca tacacttcaa agccgtttag ttggcccatt
      901 atatttaact catgtaggat taccaagcct ttttggttcc ggtttagata atttagggct
      961 tagcgaccat aacagggagt ggtacgaaac gcaggcaaac cggatggctg aacgctattt
     1021 tagtaaccat gaccccggag cactaactgc tttaccttgg gatgataaca ggtatccaag
     1081 agaatataat cctaattggt actggacatt tgcacatcca cccttaccat ttatgtggtg
     1141 gttatttttc taatcaatta acattaaaat tatgaagatg aatattttaa tcatactttc
     1201 gataatgctt tgtggtgcca gttgtgaaaa attggttgac catgtttata gtattaaaat
     1261 tcaaaataat actaatgata ccctgctttt ttatgaaagc tataattacc cagactcttc
     1321 aattgttcaa aacaagccta tattaacaag ggtttatcct aaagattata gttatttaga
     1381 tagtaaaaaa gactgggatg aagtcttggt ctctccaaaa gatactatca gcatatttat
     1441 tttaagcaag ggtacggtag acaagtatag ctgggaagca attagggctg attataaaat
     1501 tttaaaacgg tatgacttga gcatacaaga tttagaaaat aaccaatgga cgataacgta
     1561 tccataacaa aaaagcctcg gaaaaccggg gctttttgct tatagtgcag ccccgttttt
     1621 ctaaaagaaa tccttaaacc ccttacgggt aggcatcttt tcgatcagcc gggaaatggt
     1681 ttgcctgtct tccccgttag gctggcggac aggatgctgc acctgcccgg ggtatgcagg
     1741 ccgccggccc tcacctttgc ccggtgtagt tttacgccca tattaatggg tttttatatt
     1801 caaaatttta tggtattggc ctctgcacct taagcagctc ctgcttcaac gggcttactg
     1861 ctgtggattt gtgtcggaag gcttatcgtt cctatatttt ctaagttttt tctccagcct
     1921 cctttgctcc ttcttttggg ccttgcgtct tctccctatt acacgtaggc ggttaactag
     1981 cacgaatacc accagggcag ccagccaaat tggccaaatt ttgataatga aaacaaccag
     2041 ccaaaccaaa cccttccagc ctgcattaat ggcttgcttt attctttcac caaagctggg
     2101 catctcgtcg ggcctatacc tgtactcaac tttttggtaa acgtaaaccg ttatcaggct
     2161 tttgctaacc tggtggttta tgtagttcat acgaccctga agcgattcga tttcagccct
     2221 tactgcacct agctgatttt caatttttag aatgtcgtca acactggttg cctgggacag
     2281 tagtctttga tagttccttt ccaccttcat cttgttttgc aggcgggtat tcacatcaat
     2341 gtactcggtg gttacatctt cagtcgaaat ttgctggtaa tcaaccctgt gggcaatttt
     2401 tgccagctct cccagaagta ggtcaaactt ttcggccggt accctaatgc ttatgctgtt
     2461 gcccctttgc gtttcgtttt cctgaaagct ttccgaaata acagctgctt cgtacaggtt
     2521 ggcaagggag tctatttgct gtctggcaac caggtagtcc ttgacttcaa tgttgatgtt
     2581 gccgtggcgg ataatcttcg cagaaacatt cgaggaacct gaaaaatcgg aagaactctc
     2641 ctgtatgact gatttacttt cagctggcga agtttcagca gcaaagtatg cctgttcttc
     2701 agcattggtg actataactt cctccctgtt agctttgtcc ccgcaaccta gtagtaggag
     2761 ctgtagcccg aaggcaatga gatattgggt tgttttcatt ctggtaagaa ttatttatcc
     2821 taataatttc gatttatgtc aggcaatttt tttgtattgg ttcgtcggca aataccgata
     2881 atacccccgg tagccatgcg aaaaggtttg cacctgcttg tagtaaacat ctgctgttgt
     2941 cacgttggta gctgttgtaa gaagatatca gcttatttca tcaaaacggt tgctattgct
     3001 ggccgaaata tgaatggtat gcccacatgc ctggacggta tcgcccgggt ggactttagc
     3061 cctcctgcgc gactcggttt tcccgttaag ctttaccttt ccatcatcaa ccagcaggtg
     3121 agcctccgct cccgaagaag ccaggctgca tcgctttaac agcccgtcta acggtatgta
     3181 ctcaccttcc aactcaaagt taacttccat aacctatacc tgcatattta gtaaatataa
     3241 catgttttcc cggatagctg agcggaattt catgcgttca tgcattacgg gctaattacc
     3301 cggtttcgta atgggatgcg tgggtttagc cttaccgtct accgaaaccc aaaggcgaag
     3361 ggcagtatga atatcaccac aagcgtccag taaaagtaaa tattcaggta accggccatg
     3421 gtttgctcaa ccctttcggt ttctttgtcc cggaagtatg ataggaagag gtcacgtgcc
     3481 aacagcacta ggtttatcag tatcaggatg ttggatgcca gtacaaccgt tctgtttgga
     3541 gttagcccat gtgaaatcct tgctgatatt gcaaccagcg caattaggtt tgttacaata
     3601 gccagtgtgg cgagtaggag gagcaccagc acgtttctgt ccttcttcct cgatttatcg
     3661 agttcggaga tggagaacac gatgattgcc actactgcca gcagcatcac gttgaaaatc
     3721 attagggcgt ttctatcctc catgatgctt ttgggtgaaa agatcattga aaccaggtaa
     3781 acagccagcg tgatgagcac taacggggag aatatccggg ctataaccgg cgccaccttt
     3841 cgggtgatgc tggggtatat ttttatgagg cagtaggaga taattggtgc cgcaacacca
     3901 ccaaatatgg cagcattttt catgaatacc tcttcaatat tcatgttgat ggccgaaaac
     3961 agccccacgg taactaccga aaacagcaca ccggcaatca ggattaaccc ggtcataacc
     4021 gccagctcgc cgttaaaacg aatgaactca acacgccttg gtatgctccg gtagtcaaat
     4081 gaaacgaaag ctaacccgaa caggcaccaa agcagcagtg gcaggtggat gaaaaccagg
     4141 ttaatggaat cgctatccct gtggggcaac aggttggagt atataatcag cgcaacaatt
     4201 gtcataccgt aggctaaaag gcgcttccat ccggaaatcc tgttctgcca gagggtgtaa
     4261 acaataatgc cattaaagac aataatggcc aaatttctga tgtaaaaggt ttcatcaaca
     4321 ataccctcaa aaaaatcggg tagtttgact agcagtcctg ttatcagggc aatggctaat
     4381 gctactgaca ggtcaaccca cagcaaaccc ttactccggg tggtgttttc attggccagt
     4441 cttaccttcc agaaaccaac aagttcggaa ttgtagccgt ccgctatttc ggcaaatgat
     4501 tcgctgaatt cctgcctgtt ctcgcggtaa agccgctcca ggttttctgg attgttgata
     4561 ttttctatta tttgctgctt cattttttag gtatgttggt gttttatttg cagtttaaag
     4621 cccttgtacc ggggcaagtt taaatatatg tggcatgaaa accatcaggg caaatctaca
     4681 aatcgtcaat cgggtgaaat ttggccattt aatggttatt tctacttttt aaaacggtca
     4741 accaggctgt tttatttttg aatgcaccag tctccagcaa gttaattgtt agcaaccgga
     4801 aaaatgtaat ttttttgctc tataaaggta gctgatacat tgccaaaatg tacacaaagg
     4861 agtgcgttta taacattctt tagcagagca ttaccgaatt aagtggcatt tatttttacc
     4921 ggtaaaggaa gcagctgcaa aagttctgag gtttatttag aacggctttt gtaacgatga
     4981 tgttggctgg cttgttgtaa tggtattgtc tatccaaagc ttcattctgg taagtgacta
     5041 caacaaatgt ttatagcaag ggctacctga gcacgatatc acttacaatt gaggcatggt
     5101 aaaagtcgtt aattcttttg atgagctgct gtttacgcat gtggagttca tggcggagcg
     5161 ccgaggagtc aacctggatg gtgagctgtc cgtcttttag cttgattttg gtagtgtgca
     5221 tctgcacaat tcgcccaaca attttttccc agtcgccaat gatggaggtt tcgtttattt
     5281 tctcgtccca cttcatggcg gtaataaact ccctgatggc ttcgcccaac gacttggtgt
     5341 tatggttgct catggtttta tgttgaggcg attatggata cttctgagtt ttcgatacta
     5401 aaaagccgga atccggagtt gagcctgttt agtatcatat ccagccggtt cttgctggta
     5461 tcggtgatga aaatctggcc aaatccgtca tgggcaacct gattaatcag aaagccaacc
     5521 ctgtccatat cgagcttatc gaagatgtca tcgagcagca gcaggggttt aattccggag
     5581 actctcgaca ggaattcgaa ttgtgctagc ttaagggcaa tgaggaaggt tttttgctgg
     5641 ccctgtgaac cctcgcggcg aatggggtac ccgtcaatgc taagcaccat gtcgtcgcgg
     5701 tgtatgccta tggaggtaaa ctgcaaagtt ctatcccttt gaatgttgtt gcgaagcatg
     5761 gttttaaagt cgccatcgtt gaggtgcgac ctgtaggcta tggacaccct ttcttctccg
     5821 ccggataccc tgttgtagta gtccatgaaa atggggatga gctgctcaac aaagctgctg
     5881 cgcttggcat gaatggtttg ggcataccgg acaagctggt cgtcgatagc ctcaagcagg
     5941 tcgtagctaa agcttacatg cgggtggttc ttcaggatgc tgttgcgctg ggcttgtagt
     6001 cggttgtagc gcatcacctc gtcgaggtaa accctgtcgt actgcgaaat aacaccattc
     6061 atatacttac gtcgctcctc acctgactcg tcaataagcg ccgaatcggt gggggagacc
     6121 ataacgattg gaataaagcc aatatgatcg gccagccggt cgtaggtttt cccgtttcgt
     6181 cggaaaatct taccttcatc gagtttgaaa ccgcagtaaa tatcctcgtt gctcgacacc
     6241 ctttggtagc ttccctcaat tacaaaaaac tgttctccgt gcctgagcac ctgcctgtcg
     6301 ctcgagttaa aggcgctttt gcacatcgaa aggtagtaaa tggcatccaa caggtttgtt
     6361 tttccctgtc cgttgttgcc aataaagcag ttgatcttgg ggttaagttc cagctcaacc
     6421 tgtctgaacg atttgaagtt aatggccgat agatgtttaa ggtgcatggc aggtggagac
     6481 ttttgctatg ctacaaaggt aattatttgt aaaatgttgg cgcaaagttt tcagatattc
     6541 caaaatccat tacttttgcg tggacttaaa tcattactaa cccgttttaa caatggcaaa
     6601 gaataacaaa aatgctcaac ccgacacggt tgagtctatt gataacgctt taagccaaac
     6661 tgaaaggttt attgagcaga atcagaagag tttaaccatc ataatagtgg ctatactcgt
     6721 catcatcagc ctgtactttg ggtacaagcg cttttacctg gtacctaagg aggatgaggc
     6781 tcgttcacag atatttcagg ctgaatacta ctttgagaag gactcgttca agctcgccct
     6841 taacggcaac gagaacgact tgggatttgt tgatattgcc gataactaca gcatgaccaa
     6901 aacgggaaat ctggcctgct actacgccgg catttgctac cgtcagcttg gcgacttcaa
     6961 caaggccatc gagtacctta aaaagtttga tgcgggtgat attatggtta cgccaattgc
     7021 ctatggcgct attggcgact gctatgtgga gcttaagcag cttgatgagg gggtaaagtt
     7081 ctacatcaag gcagccgagt acagcgataa cgatttcacc tcaccctttt tctacaaaaa
     7141 ggcaggctta atttatgagg tgatgaacaa taacggcgat gcccttaaga tttaccagac
     7201 cattaaggat aagtatccca agagcaacga ggcaagagat attgagaagg atattgcccg
     7261 aaataaggta atacttcaaa aatagccatg atgaggggtg ctgcttgtag gcaccccttt
     7321 ctatttatta tttcccttta tgttgacttg ttggaaatca tagcttaaat ttggtaaaat
     7381 taatagctgc agtaacatgg caacgaagct taaaaatcta tccgcctacg atcccgctga
     7441 aattccttcg gcaaaaaaaa tgaaattcgg aattgtggta tccgactgga atgaggaggt
     7501 taccaaggcg ctgcttgagg gtgcatacca aacactcatt ggtaatggcg tcaaggagga
     7561 caacatactg gttaagcatg ttccgggagc ttttgagctg acccttgggg cacagttcat
     7621 ggccgaatat gccgacctcg atggggtgat ttgtctgggt tgcgttattc agggcgaaac
     7681 ccgccacttc gatttcatct gccaaagcgt tacccaggga attacccagc tgaatatgga
     7741 ttataacatt cccttcatct tcggcttgtt aaccaccgaa aatctcgaac aggctttaga
     7801 gcgagccggc ggaaaacatg gcaacaaggg cgttgaggct gcaatcaccg caattaaaat
     7861 ggcacacctg cagcaggaga tggagcagct ggaaggataa gcacaaccac cccggcggga
     7921 tttcttgcca gtataggatt tgccatttta cttgcaacaa ggttttacct tgttccaacc
     7981 acccaaggtt ttaagatttt tagttgacta taagctgcga tttggcattc tgcacttgtt
     8041 tacgctccga tgaatttggg agggctcacc gtaggggtaa taatatttgt ttggacttgc
     8101 cgaactagca aataatgccc ggttattaat ccgattgttt acaagcctta ctacatgggt
     8161 attgcaggct aacttcattc tcgcatgggg taatctccac ctcttatttt ttgttttttg
     8221 ctaaaatttc tttctatcct gcagcaagat tgcttaattc gtagtcttat atttgtaatc
     8281 taccaactgg aatttgtatg gagcatatca actacacatc ggagaatgag cgaacttttg
     8341 ccatgctttg ccatctttcg gcgttggctg gctgtatcat tccattaggg cacatccttg
     8401 gcccgctgat agtttggctt attaaaaagg atgaatttcc tgaagttgat aggcagggaa
     8461 aggctgcgct taactttcaa ctttctgtaa ccatctatgg gctcattgca ggggtgttgg
     8521 ttattctgct ggtaggcttt gttcttctgg caattctggc tgtgtttacc ctggttttag
     8581 ttattgttgc ttcggtaaaa acagccaatg ctgaaaaatt tgaatacccc ttagccataa
     8641 cctttcttcg ctagcccgaa tcacctcgtg ctttatagct gtgtttggtg ggctggtagg
     8701 tgtcagaact ctacaactta aaaaactttt agtttagggt atattaccaa aatcaatttt
     8761 ggtatatatt tgaggaaaac ataaggctat gttcaggaca aacgagtact ttgatggtaa
     8821 tgtaaagtca attgcctttg aaactcccga tggtagggct actctgggtg ttatggcaaa
     8881 aggcgagtat gaattcagca catctacaat cgaggaaatg acggttgttt cgggtgaaat
     8941 gtcggttttg cttcccggtg aaaccagctg gaaaaaatat gtagagtttg aaacatttat
     9001 tgttcctaaa gattcccggt ttagggttag agtggatggc gatactgctt accgttgcta
     9061 ttacaggtaa tttaatcggc tactcctttt tcagtcagtt tcttaaaccc tggtttttga
     9121 aattggttag taaactcacc cgaatattat ggccggttca aagggctcaa aatactatga
     9181 tgtttacctc gactacagta tttttttaaa gcgtaggggt agcaacgagc ttgtgctatc
     9241 gcaggagcaa atagagctgc tgttgttggt tgatgagctt caatccatta ctgcttcggc
     9301 taggcagatg tgtataagct accgtaaagc gtgggagctt gtgaggcagt cggagcaaaa
     9361 cctcggtttt acaatcataa gcaaatcaag gggtggcaag aatggcggca gctctgttct
     9421 aacacccgat ggcaagcttc tggccaatgc atacaggcag ctgcaagccg atttcaactc
     9481 ctcggtgaag cgggtggtta aggaattctt tcagacaatt aaccggtagc aaccatgcga
     9541 aaaattaaat ttttactact gctaattgct ccgttggtgg tgttgcagtg ccattcaggt
     9601 agaagtaagc aaaaagagct aatcgttttt cacgcgggaa gcctctcgct tcccatgaag
     9661 gcggctgccg acagctttat gaagttacat cccgatgtca gaatcctgct tgaggcagcc
     9721 ggtagcatgg agtgtgccag aaagatttcg gagcttcata aaccctgcga cctgtttgta
     9781 tcatccgact ataaggtcgt cgaaatgctt attgaccagg gttttgctac atggtgcatc
     9841 cccttcgcca gcaacgaaat ggttattgcc taccatgata agtccaggct tgccgactgt
     9901 attaacggtt ccagctggcc atccgtgctt ttgagcaaag aggtagcctt tggaagaagc
     9961 gatgagaact ccgatccatg cggttatcga acaattatga ctattagact tgccgaggat
    10021 ttataccatc ggccaggcct ggccgattcg cttcttgcca aggacgtgaa gcatatcagg
    10081 cctaaggagg ttgacctgat tgcgcttctt gaagccaacg taattgacta tttgtttatt
    10141 tataagtcgg tggcgcagca acatggacta aactatcttg aactccccgc tgcaattaac
    10201 ctttccagta aggagtacga atcggtttac tccagggttt cggtagaggt ggttggtagc
    10261 acgcctgatg caagggtagc tttaaccggt gagcctatgg tatatgggtt aacaattccc
    10321 cgtaatgcac caaattttga tattgctgtg cagtttacag cgttttttct aagcgaaaat
    10381 agtggtatgc gcattctttc tgcgatgggg caaaccccga taatccctac atcaaccaaa
    10441 acctataaca acataccaca agcattacgg gcatttgcaa ccccgtagca caaattcaac
    10501 tcatcaaggc tgtttgtata gctccttcgg tctcactaga acagtgcaat tttagaagta
    10561 gtagcttatt aatccggtct gtagccgctg ccaccgctcc tgctccgtca tggagttgta
    10621 tttctgcttt tcctcctcaa ttcgccgacg ttcttcctcc tgctttttac gttttagctc
    10681 ctcaaagtga gccacacgtt tatccctaag atatttttcg gttacgttgg ggaaaagctc
    10741 cagcaacagt tcttcctttt cgttcatggc cagcttaacc ccttcgccat actcgggaaa
    10801 tgaagggttg ggttgcttct tgtagctggc agtgttaaaa ggcacctctt cggcagagcc
    10861 ggtaattttc agacgaaatt gggggtcaat gggaagggga gttttgccgt aggagccttt
    10921 gaccaggttg acaaattggg ttgattttgt ggtgtagaaa ggaagtccct tgttttcgtc
    10981 gattatgcag ttaacggcct gcgctccaac tatttgactc gtaggggtaa caagtggagg
    11041 gcaaccagca tccaaacgga cagttggaat aatttcgagc acgcgtggaa gaagcttttc
    11101 caactttagc tgtttaagct gagccagcat gttggtgtac attcctccag gaatttcggc
    11161 atgcttaacg gtttcgtcgg gagcggggaa gccaaagtaa ctctcaattg cctggcaggt
    11221 tttggttagc tcgtcgtatt gggccgataa ggcttgttgt atggcttgtt caaaaaggct
    11281 gtcaacctgt aaagggaggt tagggtgggt gatgtcaaat tcgtggggta gggggtaggt
    11341 atcgaagctt ttcagctcgg tgcggatgct ttttagctcg gtgttgatgg ttgctgctgc
    11401 gttaaggtta atgcccgttt caattcccaa cttttggcag aaaatttgaa tcagctcgaa
    11461 tgcaggagca gcagggccgc cggcaaagtt ccaaattgcg gtgtcaacaa tatcaacacc
    11521 attgacaatg gcagcaaggg ttgcagccaa tccatatcca ggtgtacagt gggtgtggaa
    11581 gttaatagga accttgattt cttttttaag cgtcgaaatt atgctggcgg ccctatcggg
    11641 ggtaaccagt ccggccatat ccttaatggt aatcatatcg gcgcccatgg cttccagctc
    11701 ctttgccttc gataagtagt aagcttcagt aaaaatttta cctggaagtt ttttcctgtt
    11761 gattacggcc ttaactctat cctttttgga aaatttgggg tcaacggtat agcaaactgt
    11821 gcagtcggcc aaaccaccat tctccttaac atactttacg gtgctacgaa tattgttggt
    11881 gtcattcagg gcatcaaata tcctcataat ggatatgcct gagtttacag caattctgtt
    11941 gaatccctct ataacctcct cagggtaggg gttgtagccg aaaagatttc gaccacgtga
    12001 cagggctgtc agcttagttg agtcgccaat taccttttta atactttcta gccggtacca
    12061 gggatcttcg ttcaaatacc tcattatgga atcgggaaca gcgccgcccc aaacctccat
    12121 tgcgtagaat ccggcttcct tgaagtaagg taaaacctgt tcaacctgtt tttgggtcat
    12181 tcgtgttgca aacagcgatt gctgcccatc gcgcagggtt acatccctga tgagtatttt
    12241 tctggggttg agtgccatgg tgtagttgtt gtattgaagt tgacttgcta gttgctgatg
    12301 cgctaacgca tactttattg tttgttacgg caaagaaact aataagttat ctgaaacccg
    12361 cataaatatt tgaagttcta caacatagct ctttaacata tttatataac ataaaaaaag
    12421 gggctaaact ggccccttta tactagtagc tatataacta gtttgtattc agcatgttga
    12481 aattatcgct ttttccgtac agcatcctga cgcttttgta gctcctgctg acgcttagcc
    12541 acttcctcca tcctcatttg ccattttgat ttggttacgg gttttttggc attttcatgc
    12601 agcttggcca gcaaggcttc gtcatcaaca aaacgtctca taagcacagt ttgaagcatg
    12661 gatataaggt tggtaagcag gtagtagtag ctaagggctg cggagtagtt gttgaaccag
    12721 aacatcatca taacaggcat caaccaggtc atcataaact tcattccggg catctgggcg
    12781 ttagtgtcgg ccatctggtc gttactcatt ttggaagtta tgaccattga gattgccatg
    12841 agtagggcaa agaggctgat gtgatcgcca aaccatggga tggtgaaggg caggtggaga
    12901 atggagtcgt atgacgaaag gtcttcggcc catagaaagc tctgctgcct gagttcgaat
    12961 gatgcgggga agaagcgaaa catggcaatg agaatgggga attggatgag gacaggaagg
    13021 cagcctccca ttgggctcac cccaaccttt ttgtaaagcg ccatcacagc ttgctgcttc
    13081 ttcatggcgt cctccttctt ggggtacttc ttgctgattt cgtcaatctg gggcttcaaa
    13141 accctcatct tggccgagct gaggtacgac ttgtaggtga gcggcagcag caccagcttg
    13201 atgatgatgg tcattatcag cacaatgata ccatagttgg ttagaaaact gcttaaccag
    13261 ttgaatagca gaatgatgac gtacttgttt atccagcgga caatatttcg tcccagcggc
    13321 accacttttt caaacccctg gttgtattcc tttagggttt tgtagtggtt ggggccaaag
    13381 taaaagccca acttcaaggt ttcgtcgttc tgatttttaa aagggagcga taaccgggca
    13441 tccatggcct tgacgtgcag gctatcgttc aggttggtca tctttaggtc ggagttgatg
    13501 aagtgctcat tggcaaccag tatggatgag aaaaactggt gtttgaagtc aacccatttt
    13561 aacttggtgg ttagttcttt cttttcgtcc tggctgcgcg ggtttagctc ctcatagtcg
    13621 ttgttcagga aggtgtaggc tatggtggtg tagttacgtt cattctctat tcccttttcc
    13681 agcctgttga gcttgaggtt ccacaggaga tcaattgaac cggcttgtcc gccaatggac
    13741 ttggtcatcc cgttaaagcg gatgtcaaaa tcaaccatgt agctgttggg cttaacggtg
    13801 tagatgtagt cgatgtagga ggaatcggca atttgaagtc gcatggttag tgaggccggt
    13861 tggctttccg atgcggtaac tgcggtatcg gaggtttggg gaacaaagaa gaggtcatta
    13921 gttttaacgg cgttgttttc accccagaac tgtaggccaa attcgttgtc atcgccgtta
    13981 aaaagtacca gtggctcacc ggcatgggtt ttaaactttt ttagctcaac ggagtatatt
    14041 ctgccacccc tattcgacac caccacgcgc atgaggtcgt tttccaggct tatgaacttg
    14101 cgttcacctt ccagggcagg ggcaaatgag cctacctggg actgaacttc ctttttctga
    14161 atggtgtcag caagcatggc agccttagcc ttttcctctt cggctctttt ctgttcatac
    14221 tcggcttgca ccctggctaa cgaatcggct ttacgtacgt aggcttcacg ctcttctttt
    14281 gaaggtgcgg caaggtaccc aaagccaatc atgattaaga aaataagcac taaacctaat
    14341 attgtattcc tgtccatctg ctgctgattt tctggttgtt taagtgaaaa aaagccatcc
    14401 ttttagggtg gcaaaggtag taatatttgg atggttagcc accaaaattc atggtggctt
    14461 taacaaattc aacaaaaagc ggatgcgggt taataaccgt gcttttatac tccggatgga
    14521 actgcacccc aacgaaccac ggatgagaag aaatttccat tatttcaacc aggttggttt
    14581 ctggattaat gccggctgcg accatgccgg ctgcctcgaa ctggggcaaa aactggttgt
    14641 taaactcgta gcggtgccgg tgcctttcgt gggtttctag tttatgatag gccatgtagg
    14701 ctttagttcc cttttttacc tggcaggggt aggagccaag ccgcatggtg ccacccatgt
    14761 cggtgattcc cttttgctcc tccatcaaat caattaccgg aaagggcgta ttgcgcacca
    14821 tttcggtaga gttggcctcc tgcaagccca gaacatttct ggcaaactca actacagcac
    14881 actgcattcc caggcaaatg ccaaagaaag ggacttgatt ctctctggca tatcgaatgg
    14941 cttcaatttt gccctcaact cccctgtgac caaaaccggg cgctaccagt atgccctgca
    15001 ggtggcccag cttctctttt acatttgatg cggtgatgct ttccgattga atcatttcga
    15061 gctcaacatg gcattcgttt accgctccgg catgaataaa cgactcaaca attgatttat
    15121 aggcatcgtg tagctcaacg tactttccaa ccaacccaac ttttactttc gacttcggat
    15181 ttttaagctt gtttataaac tcgttccaat cgtcaagttt gggaggttcc tgaacaggca
    15241 ggttgagttt tttcatgatg gtctcgtcga gtttctcctt atgcataagt agcggaacct
    15301 cataaatagt ttttacatca atagactcaa ccactgccga ggaatcaacg ttgcagaata
    15361 gcgctacctt gcgacggatg tcatcattta agggtttctc tgttcttagt accagaacat
    15421 ccggttggat accattttca agaagcactt taacggaatg ctgggtgggt tttgttttaa
    15481 gttcacccga agctgacagg tagggcacca gtgtaagatg gatgaatgcg gagttggttg
    15541 gacccagctc ccacctaagt tggcgcaccg cttcaatgta aggtagcgat tcaatgtcgc
    15601 ctactgttcc accaatttcg gttatcacca catcgagctt atgcttcgtt ccaaggagtt
    15661 ttattcttcg ctttatctca tcggtaatat ggggaataac ctggacggtt ttacccaggt
    15721 aatcgccgtg cctctccttg cttataacag tttggtaaat tttaccggtt gtaacgttgt
    15781 tcatctggga tgttgggagg tttaaaaacc tctcgtagtg tcccaggtca aggtctgttt
    15841 ctgccccgtc ctcggttacg tagcactcgc catgctcgta tgggtttaga gttcccggat
    15901 ctacgttgat gtatggatcg agtttttgaa tggttaccga gaaacctctg ctttgtagca
    15961 acttcccaag tgaagctgat attatcccct ttcccagcga tgaggcaact ccgccggtaa
    16021 caaaaacata cttggtgttg tgtggcatgg cttgaacttg ttttaatcta gcttaacaga
    16081 aaaattcaag gataaaggat aaaaaataaa ataaacttgt ccgcctccgg gcggaacttg
    16141 tccgcctact ggcggattca aaatttcatt ttcaaac
//
LOCUS       DAOSHM010000052        16166 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_2951_length_16166_cov_19.696543, whole genome shotgun
            sequence.
ACCESSION   DAOSHM010000052 DAOSHM010000000
VERSION     DAOSHM010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 16166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16166
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2951_length_16166_cov_19.696543"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..726
                     /locus_tag="PK839_08205"
     CDS             <1..726
                     /locus_tag="PK839_08205"
                     /inference="COORDINATES: protein motif:HMM:NF023179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HOG72754.1"
                     /translation="VRSRVRRRRPSGKVGLGAFVFNDNNGRIHRTGFQGTYSYHIYMR
                     DYQLSFGASISAFQFKANVTSQDGYTQENDMLLVGKTSKFAPDANVGVLFSGNNFYAG
                     LSITSLFQSSIQFGGGNSETAYRLLRQYYIVGGYRYQPYRSDYAIEPSILFTTTERLQ
                     LNADFNVKGYYKDNYWVGVSVRTSGAVVTMIGMKYQQFYFGYAYDYNFSDVSNISSAG
                     SHELMIGLKLGDSARRYRWLNRF"
     gene            1577..1963
                     /locus_tag="PK839_08210"
     CDS             1577..1963
                     /locus_tag="PK839_08210"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOG72755.1"
                     /translation="MDGKEKILYVDDETINLELFKINFRNEYDVVVANSAIKGLEILK
                     GEPIKVVVSDLKMPVMNGLEFIEKVKGDNPARVCILLTAFMESDVMLQAINKELIFRY
                     IMKPWKKAELEEVIRLALVRHASLAG"
     gene            complement(1952..4528)
                     /locus_tag="PK839_08215"
     CDS             complement(1952..4528)
                     /locus_tag="PK839_08215"
                     /inference="COORDINATES: protein motif:HMM:NF025069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR/HmsC family protein"
                     /protein_id="HOG72756.1"
                     /translation="MFTRKVPVALAILCLLAFPVRPKSSEGDLPQVSEARVKAAVILN
                     IAREVRWPNERSIENFRVGVMDADTILLHELHKVEPSYLIRGKPFNTFYFSNESQLEN
                     IDILYLSSEFSKNLFRVYRELEGRPTLIITDGASDRALTMINLTYNAEKKAIAFEVNR
                     ENMESRGFRVGQELLLLGGSYMDIKELYVETSKRLNEATSLMAKYQEELDQLGREHDE
                     YLLQVEKLGNQLANLSDELKRTEQDYSNLTLRLHDRDSLLGVRNRELQLRINESTLLQ
                     GLIQTQLDSIKNSSTLLDSLNQLVAEKQKELQAQQNLINNQGQVITEKESTILQQQRK
                     WYITLILLLGVLSSLIFASISYTTKRRMNLRLENLVEERTRELNASREHFKNLFENSP
                     VAMIELNFSALKDFSWQSDNGKVDIDSMSNEEFSALMYRALTYSRVVDANNAAIKLFG
                     YADKKDFMENPSKTLTFQSLVDYRSLAKLYFNNQPLAKFETTRQTTSGETIHAVFEWL
                     VLPGYENTYERVLLAITDITALKKYEKELTRHKDHLEEIVLERTREIIKLNEDLTSTN
                     AELKQKTEELTQTIQKLGEAQNQLVRSEKLASLGMLTTGIAHEINNPLNYISGSQQAL
                     GTLLEEMWKLLNSYRDIIATKLTSDFLINIEKEHNQNINDLYFSMKFLLSNIETGISR
                     ANSIVKSLRVFLGESSQEMAEYSIPNAVGSILTILKSSYFGKIRITEEYSPDLPLIHC
                     SAEGVNQVIMNLLTNAIDAIPEKGEINITATYEVDDDCIAVQVRDNGMGISKENINRI
                     FDPFFTTKETGSGTGLGLYLAYNIVIAHHGSIEVDSAEGVGTTFTLRLPRMVPNGLEL
                     PS"
     gene            4802..6352
                     /locus_tag="PK839_08220"
     CDS             4802..6352
                     /locus_tag="PK839_08220"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019131137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HOG72757.1"
                     /translation="MTQDDLFKKLISHCKEYGFVFPSSEIYDGLSAVYDYGQNGVALK
                     NNIRRFWWEAMTRVNENIVGIDSAIFMHPQIWVASGHTSAFNDPLIDNRDSKKRYRAD
                     TLIEEWIQKQEEKVEKEVEKARKRFGDSFDEQLFRQTNPRVVELSEHAKTVNQRMVQA
                     LEANDLAAIKKIIEDCEIACPISGTRNWTDVRQFNLMFETKLGSVSDEANTIYLRPET
                     AQGIFVNYLNVQKTGRMKIPFGIAQVGKAFRNEIVARQFIFRMREFEQMEMQFFVKPG
                     EEMKWFEYWLDKRMRWHKAMGFGDGKYRFHKHEKLAHYANAASDIEFEFPFGFKEVEG
                     IHSRTDFDLSNHQKHSGKKLQYFDPETNESYIPYVIETSIGLDRTFLLVMSAAYTEEK
                     LTKDDGTADERVVLRIPPALAPVNLAIFPLVKKDGLPEMARKIMDDLKYDFTCQYEEK
                     DTIGKRYRRHDAIGTPFCITVDHQTLDDETVTIRYRDSMEQDRVPASKLREIMGEKVS
                     LTNLLKQV"
     gene            complement(6411..7025)
                     /locus_tag="PK839_08225"
     CDS             complement(6411..7025)
                     /locus_tag="PK839_08225"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007134449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HOG72758.1"
                     /translation="MHIAVAGNIGSGKTTLTSLLAKYYKWKPQYEDVDDNPYLNDFYE
                     DMERWSFNLQIYFLNSRFSQIVSIHRSGEDVIQDRTIYEDAHIFAPNLHQMGLMSSRD
                     FNNYLNLFNLMTSLVKPPDLLIYLRASVPTLVRQIQKRGREYEKNIRLDYLQRLNERY
                     EAWIEGYNLGKLLIIDVDHLDFSEKPEDLRTVINRIDAQIHGLF"
     gene            complement(7173..8702)
                     /locus_tag="PK839_08230"
     CDS             complement(7173..8702)
                     /locus_tag="PK839_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HOG72759.1"
                     /translation="MPLVNSLLNRINSSRLRSIDYFRRHPQEVQEQTLFNLLSRAKNT
                     EYGQLNGFSRFKSIADFQQGLPVMEYEDFKPYIDRMRSGEENLLWPGKISWFAKSSGT
                     TQNKSKFIPVSREGLNGCHFRGSRDVIAIYFDLYPKNGLFRGKGLTLGGSHQVDTLSG
                     SSRYGDLSAILLQNQPWWTDFIRTPSQRVALIPDWEQKLEKITGETLAQNVTSITGVP
                     SWNLVMIKHILNHTGKSNLLEVWPNLELFIHGGVSFTPYRDQYFKLIPSPQMHYMEAY
                     NASEGFFAIQDNPETDDMLLMLDYGVFYEFIPAEQLGQPNPPAYPIGEVEVGKNYAML
                     ITTSSGLWRYLIGDTVTFTSKSPYKIKITGRTRHFINAFGEEIIIDNAEKAIKKACDA
                     TGAQVAEYTAAPVYMETDKKGSHEWLIEFNVDPASLDEFSRLLDAALCEVNSDYEAKR
                     YKNITLDFPLVRSLAKGTFFEWMKRNNKLGGQNKVPRLSNNRDYANQLLAIHRELSSA
                     S"
     gene            complement(8944..9570)
                     /locus_tag="PK839_08235"
     CDS             complement(8944..9570)
                     /locus_tag="PK839_08235"
                     /inference="COORDINATES: protein motif:HMM:NF013488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HOG72760.1"
                     /translation="MQVEFFHDVLCAWCFALSPRMRRLVAEFPEIEVIHRSFALASDA
                     DRLVEMFGSKEAAKREILNHWRNANENDDEHRIHTEDMALRTFDYPYSMPGLLACKSA
                     ELQGGQLAHWNMFDRIQKAHLVETQNIADFNVLLQCADEVGLDTERFKNDFESDATLD
                     AIYQDSYRAKELGVNAVPSLLINGRKLLSGAHKYETLKELTQRELIGK"
     gene            complement(9592..10440)
                     /locus_tag="PK839_08240"
     CDS             complement(9592..10440)
                     /locus_tag="PK839_08240"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HOG72761.1"
                     /translation="MKRAITLSIFAIAIAALISCTGNKSNKAGETSTPPTMQPVVKGT
                     EITYYDGSTPLKGYIAYDESLTGKRPGILVVHEWWGNNDYSRMRANMLAELGYVAMAV
                     DMYGNGLILDNPKDAQNTANQFYTNPALMKERMLVAYNTLIADSLVNAERIAAIGYCF
                     GGAVVLNSASMGVPLDAVVSIHGGLTGFKATPEMKNVNVLICHGGADPFVPQADIDNF
                     KSEMQNMGVNYEFKVYEGATHAFSNKASTEAGKKFNMPISYNAAADSASWSDMVTFFN
                     KYFPAK"
     gene            complement(10828..11784)
                     /locus_tag="PK839_08245"
     CDS             complement(10828..11784)
                     /locus_tag="PK839_08245"
                     /inference="COORDINATES: protein motif:HMM:NF022720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3078 domain-containing protein"
                     /protein_id="HOG72762.1"
                     /translation="MSTKKDFLLVIPFLMISNLITAQISETVVKELKTQSEDSILGWK
                     KGFVTGINFSQTSLTNWAAGGQNSLSLATMLSGFVSFKNEKVAWDNTLDIGYGFLNQN
                     GVKYNKKTDDRVDLSTKYGYRASKNIYYALLLNFKTQMAKGYDYPNDSTRNMISNFFA
                     PAYLVGALGMDIKPSSYLSLFVAPFTGKFTFVTDSILSSTGAFGVKPGKEYRSEFGGY
                     VRISFSKADFKPEVLKNLSFTTKVDFFSNYADKPQNIVVNWEALVAIKVNKYLNMNLS
                     TMLIYDDKIKINIDKNGDGTFDSKSPRVQFKEILGVGLSVKL"
     gene            complement(11821..13860)
                     /gene="uvrB"
                     /locus_tag="PK839_08250"
     CDS             complement(11821..13860)
                     /gene="uvrB"
                     /locus_tag="PK839_08250"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005846639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HOG72763.1"
                     /translation="MNFKLVSDFQPSGDQPEAIKQLTDGLGRGDKFQTLLGVTGSGKT
                     FTIANVIAQLNRPVLVLSHNKTLAAQLYGEFKSFFPENAVEYFVSYYDYYQPEAYLPV
                     TDTYIEKDLSINDEIEKLRLSATSSLLSGRRDVIVVSSVSCLYGIGNPQDFYNGTVRV
                     KVGQHLGRNQLLRQLVDGLYARNEVEFKRGTFRVRGDSVDIYLAYGDKGIRVIFWGDD
                     IEALEMFDPVSGVSIETMEEAVIFPANIFIAGKSRINQAIAQIQDDMMRQAEHFRSIG
                     HMVEAKRIQQRVEYDLEMIKELGYCPGIENYSRYFDGRQAGMRPFCLLDYFPNDFLTI
                     IDESHVTLSQVRAMYGGDNSRKQTLVEYGFRLPAAIDNRPLRFDEFEGLIGQTIFVSA
                     TPSDYELRLCQGVVVDQVVRPTGLLDPPIIVRPSLNQIDDLIGEINASAKRDERVLVT
                     TLTKRMAEELVKYLVKFDIRCRYIHSDVDTLERVQIMEDLRKGLFDVLVGVNLLREGL
                     DLPEVSLVAILDADKEGFLRSARSLTQTAGRAARNLNGRVIMYADKITGSMQLTMDET
                     NRRREKQLKYNELHGITPTQIVKPKKSIMAGKLSTGEVVKPYVEPDTVDFAADPVVQY
                     MTKPELAEAIERARKEMEREAKDLNFFEAARYRDEMFALQRLFETKFGAKKSKST"
     gene            complement(14226..14660)
                     /locus_tag="PK839_08255"
     CDS             complement(14226..14660)
                     /locus_tag="PK839_08255"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOG72764.1"
                     /translation="MTTLEQYNWSNRLILIVDDDRASSMLLELIISKTGAKMIFVSNG
                     RSAVDTFLSTHGIDLILMDIKMEGLNGLDATRLIRERDPHVPIIAQTACVIAGDKEKC
                     LQAGCNDYIPKPININKLMETMNKYLSHTNPIVKVNDLFSEN"
     gene            complement(14822..15565)
                     /locus_tag="PK839_08260"
     CDS             complement(14822..15565)
                     /locus_tag="PK839_08260"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOG72765.1"
                     /translation="MGKTITAFVVDDEANSRDILVQMLSFDKDIQVLGASENIDQAMQ
                     AVREHNPDILFLDIEMPGGSGFELLERLRKTPYNPAVIFITAYNQFAIKAIKYAAFDY
                     LLKPIDLDDLVDTLDRYKVNIDRGTMKAKADKLLSCLPEFRKLKFSMRRGIIFIEPEE
                     ITWCEACGSYTILHFHSRKDEVVSVSLKDVESQMEGLPFLRVSRSAIINLKYLAGIDR
                     RNKQCIVQKGDKTYRVPGTNSILKILETR"
BASE COUNT         4031 a         3715 c         3883 g         4537 t
ORIGIN      
        1 gtgaggtcaa gggttcgccg tcgccgtcct agcggaaaag ttggattggg cgcttttgtg
       61 tttaacgaca ataacggccg aattcaccgc acaggttttc agggaaccta ctcctaccac
      121 atctacatgc gcgactacca gcttagcttt ggggcttcca tatcggcttt tcagttcaag
      181 gccaacgtta ccagccagga tggctacacc caggagaacg acatgctgct ggtgggcaaa
      241 acctccaagt ttgctcccga tgccaacgtg ggggttctgt ttagcggtaa taacttctat
      301 gccgggctat ccatcacctc gctgtttcaa agttcaatcc agtttggcgg aggaaactcc
      361 gaaaccgcct accggctttt acggcagtac tacattgttg ggggttacag gtatcagccc
      421 taccggagcg actatgccat tgagccctcc attctgttca ccaccaccga acgcctacag
      481 ctgaatgccg acttcaacgt aaagggctac tacaaggata actactgggt tggggtttcg
      541 gttcgcacat cgggggcggt tgttaccatg attggtatga agtaccagca gttttacttt
      601 ggctacgcct acgactacaa cttttccgac gtatcgaata tctccagtgc tggctcccac
      661 gagctgatga tcggcctgaa gcttggcgac agcgcccgcc gctaccgctg gctgaacagg
      721 ttctagcaag gaagaaaagg ataaaggtta atacaaggat aaaggttaaa gggtaaagga
      781 taagataatt caagattcaa gaacctgtcc gcctcctggc ggaacttgtc cgcctgctgg
      841 cggatttaag ccacctgccc atttgcgggt ccggctttta tgccatttta ctaataccag
      901 caacgttcat cgtttcccgc cttaccgggc accgcggaga ataccttttt cccgttgggg
      961 ctgaagaacg aggataaagg ttgatgggaa gaaatccccc atgaataagc taacggtaaa
     1021 aacccacaac aggctaagtg tttaggaaac agggttttaa aggtgacaaa tatttacttg
     1081 ttaccgctga ttttacctgt gctgctacca aaatgtattt ttttcgtcat atttgagcca
     1141 tgaaaaattt atggcgtgta aagtgggtga agttatccct tatactgtta ggttgggtta
     1201 ctattaccct tttaattcct gcagctttac actttagcct tccggttgtt ccctttgatg
     1261 attttcattt ccataccgat tacctggtgc attttttgat ttttgcgatg ttgactgccg
     1321 ctgcttcgct ggccaaccta cgcattaaac cggtaattct atttcctttt tttttggcaa
     1381 cagcagcctt ggtggaagtt tgccagctga aaatcccatc gcgtacctac aacccgtacg
     1441 acttaatttg taacctttcc ggcgtagcct tttcattaat cgtcttttat aaaaggttta
     1501 aaaatcatca ttccgattaa ccttgtaagc taaattttag taaatttgtt tatattgata
     1561 aaaactttcg ggtaacatgg acggtaagga gaaaattctt tatgttgacg acgaaaccat
     1621 taaccttgag ctgttcaaga ttaactttcg caacgagtat gatgtggtag tggccaactc
     1681 ggccatcaag gggttggaaa ttttaaaagg tgaaccgata aaggtggttg tgtcggactt
     1741 aaagatgccc gtgatgaacg ggctggagtt cattgaaaag gttaagggcg ataaccccgc
     1801 tcgggtttgc atactgctta ccgccttcat ggaatcggat gttatgcttc aggctattaa
     1861 taaggagttg atattcaggt atataatgaa accttggaaa aaagccgagc tggaggaggt
     1921 aatcaggttg gcgctagtcc gccacgcgag cctagctggg tagctccaac ccgtttggta
     1981 ccatgcgtgg cagccgaagg gtgaaggttg ttccaactcc ttcagcagaa tccacctcga
     2041 tggagccatg atgtgcaatc acgatgttat atgccaggta aagccctaac cctgttccgc
     2101 tgccggtttc cttggttgtg aagaaggggt cgaatatcct gttgatattt tccttcgata
     2161 ttcccatgcc gttatcccta acctgcacag ctatgcagtc gtcgtcaact tcgtaggtag
     2221 ctgtgatatt tatttcgccc ttttcgggaa tagcatcaat ggcattagtg agcaggttca
     2281 tgataacctg gttaaccccc tctgcactac agtggatgag tggcaggtct ggggagtact
     2341 cctcggtaat tctgatctta ccaaagtagg atgattttaa aatggtgaga atacttccaa
     2401 ccgcattggg gatgctatat tcggccattt cctggctgct ttcacctaaa aacaccctca
     2461 ggcttttaac gatggagttg gccctgctga tgcccgtttc aatattggag agcaggaatt
     2521 tcatggagaa gtaaaggtcg ttgatattct ggttgtgctc cttttcgatg tttattaaaa
     2581 agtcgctggt aagcttggtt gcaataatat cgcggtagct gttaagcagc ttccacattt
     2641 cctccagaag tgttcctagt gcctgctggc tgccgcttat gtagtttagg gggttgttaa
     2701 tttcatgggc tattcccgtt gtgagcatac ccagcgaggc caatttttct gaacgtacaa
     2761 gttgattctg cgcttcgccc agcttttgaa tggtttgtgt aagctcctcg gttttctgct
     2821 tcagctcggc gttggtggaa gtcaaatcct cattcagctt gataatctcc ctggtccgtt
     2881 ccagaacaat ttcctccaga tgatctttgt gccgggtaag ctccttctca tactttttaa
     2941 gggcagtaat atcggtaata gccaaaagaa ccctctcgta ggtattttcg tagccgggaa
     3001 gcacgagcca ttcaaaaaca gcatgtatag tttcgcctga agtggtttgc ctggtagttt
     3061 caaactttgc aaggggctgg ttattaaagt agagtttggc cagcgaccgg taatcaacca
     3121 gcgactggaa ggttagcgtt ttggagggat tctccatgaa gtccttttta tcggcatagc
     3181 caaaaagttt aatggcggcg ttgttagcat caaccaccct ggagtaggta agggcacggt
     3241 acataagtgc tgagaactcc tcattgctca tgctgtcgat atccaccttg ccattatcgg
     3301 attgccatga gaaatctttc agggccgaaa agttcagctc tatcatggct acgggggagt
     3361 tttcaaataa atttttaaaa tgctcccggc tggcatttag ctcccgggtg cgctcctcaa
     3421 caagattctc aagccgcagg ttcattctcc gcttggtggt gtatgaaata gaggcaaaaa
     3481 tcaggcttga taaaacaccc aaaagcaaaa tcagggtgat gtaccatttc cgctgctgct
     3541 ggagaatggt ggactccttt tccgtgatta cctgcccctg gttgttaatg aggttttgct
     3601 gggcctgtag ttctttttgt ttttcggcta ccagctggtt tagcgaatcg aggagggttg
     3661 aagaattttt aatggaatcg agctgggttt gaattaaccc ttgcagcagg gtgctctcgt
     3721 taattcttag ctggagctcc ctgttgcgaa ctcccagcaa gctgtcccta tcgtgcagcc
     3781 ttagggttaa gttgctgtag tcctgctcag tccttttgag ctcatcggat aggttggcca
     3841 gctggtttcc taatttttca acctggagca ggtattcatc atgttcgcgt ccaagttgat
     3901 ccagctcttc ctggtatttg gccatcaggg aggttgcctc attcagccgt tttgaggttt
     3961 ccacgtatag ctccttgata tccatgtagc ttccgccgag cagaagcagc tcctgcccaa
     4021 ccctgaagcc gcgtgattcc atgttctcgc gattaacctc aaaggcaata gccttttttt
     4081 ctgcattgta tgtaaggtta atcatggtta gcgccctgtc ggaggcaccg tcggtgatga
     4141 ttagggtagg cctgccttca agctcccggt aaactctaaa aaggtttttg ctgaattcag
     4201 atgaaaggta gagtatgtcg atgttctcca gctggctctc attggagaag taaaaagtgt
     4261 taaagggctt ccccctgatg aggtatgagg gctcaacctt atgtagctcg tggaggagga
     4321 tggtatcggc atccatcacc cctaccctga agttttcaat actcctctcg tttggccacc
     4381 tgacttctct ggcaatatta aggataaccg ctgctttaac cctggcctcg gatacctgag
     4441 gcaggtcgcc ttcagaactt ttcgggcgaa ccggaaaagc cagaagacaa agtatggcta
     4501 gtgcaacggg aactttcctt gtaaacatat tctaaaactg ggtgaattac ctgcaattta
     4561 caaaacaatg gatgaaagtc aacatgcagg tgtatattta tgccctgctc atacaagttg
     4621 caaaccagga atggaagcgt cggtgttggt tgcttttcct ttccgcctta catacaggga
     4681 tttacgatta attgtgctga gcagtatgtt ccgggtgatg taaattttca atttgacagg
     4741 atggcaattt gagctacttt tgcggtttac tcttacttag ctaacaatat acagctgcta
     4801 tatgacacag gatgatcttt ttaagaagtt aatatcccac tgcaaggagt atggatttgt
     4861 gtttccctcc agcgaaattt acgatggttt gagcgccgtg tacgactacg gacaaaatgg
     4921 cgtggcgcta aagaataaca tccgtcgctt ttggtgggaa gccatgaccc gggttaatga
     4981 gaacattgtg gggattgatt ctgccatttt catgcacccc cagatttggg tggcatcggg
     5041 gcataccagc gccttcaacg atccgctgat tgacaacagg gacagcaaga agcgctaccg
     5101 tgccgatacg cttattgagg agtggattca aaagcaggag gaaaaagttg aaaaggaggt
     5161 tgaaaaggcg cgtaagcgtt ttggagatag ttttgatgag cagcttttcc gacaaaccaa
     5221 cccccgtgtg gttgagctgt cggaacatgc caaaaccgta aaccagagaa tggtgcaggc
     5281 gcttgaagcg aatgacctgg ctgcgataaa aaaaataatt gaggattgtg aaattgcctg
     5341 tcccatctcg ggcactcgta actggactga cgtaaggcag tttaacctga tgtttgaaac
     5401 caagctgggt tccgtcagcg atgaggcaaa caccatatac ctgcgtccgg agaccgccca
     5461 ggggatattc gttaactacc tgaacgtcca gaaaaccggc aggatgaaaa ttccatttgg
     5521 tattgcccag gttggcaagg ccttccggaa cgaaattgtt gccaggcagt tcatttttcg
     5581 tatgcgtgaa tttgaacaga tggaaatgca gttttttgtt aagcccggtg aggagatgaa
     5641 atggttcgag tactggctcg ataagcggat gcgctggcac aaggccatgg gtttcggcga
     5701 tggaaagtac cggttccata agcatgaaaa gcttgcgcat tacgccaatg cagcctccga
     5761 tatcgagttc gagttcccat ttggcttcaa ggaagttgag ggtattcact cccgcaccga
     5821 ttttgacctg agcaaccacc agaagcattc cgggaagaag ctgcagtact tcgacccgga
     5881 aaccaacgag agctacattc cctacgtgat agaaacctcc atagggctcg accggacctt
     5941 cttgctggta atgtcggctg cctacaccga ggagaagctt accaaggatg acggcactgc
     6001 tgacgagaga gttgttctcc gtattccgcc tgcccttgcc cctgttaacc tcgcaatttt
     6061 cccgctggta aaaaaggatg gactaccaga aatggctcgt aaaattatgg atgatctgaa
     6121 gtacgacttt acctgccagt atgaggagaa ggataccatt ggcaagcggt accgccgtca
     6181 cgacgccatt ggaactccat tctgcatcac ggttgaccac caaacgcttg acgacgaaac
     6241 ggttaccatt cgctatcgcg acagcatgga gcaggaccgt gtgcctgcgt caaagctcag
     6301 ggaaataatg ggcgagaagg tttcgcttac caacctgttg aagcaagttt aaccctatca
     6361 tccggagcga gaggctccga tacgttgctt tttggaagac ttgctacttt tcagaaaagt
     6421 ccgtggattt gcgcatcaat cctgttgatt accgtgcgca ggtcttcggg tttctccgaa
     6481 aagtcgaggt ggtcaacatc aataatcagg agttttccga ggttgtagcc ttcaatccat
     6541 gcctcatacc gctcgttaag ccgctgcagg tagtcgagcc ggatattctt ctcgtactcc
     6601 cttcctcttt tctggatttg cctgaccagc gtgggaacag atgctctaag gtagatgagc
     6661 aggtcgggag gcttcaccag cgaggtcatc aggttgaaaa ggttgaggta gttgttgaaa
     6721 tcgcgactcg acatgaggcc catctggtgg aggttggggg caaagatatg ggcatcttcg
     6781 taaattgtcc tatcctgaat tacatcctcg ccgctccggt gaatgctcac aatctgggag
     6841 aaacggctgt tgaggaagta aatttgcagg ttgaagctcc accgctccat atcctcgtag
     6901 aaatcgttca ggtaggggtt atcatccaca tcctcatact ggggcttcca cttgtagtat
     6961 tttgcaagca agctagttag cgtggttttc cccgaaccaa tgttaccggc aactgcaata
     7021 tgcataatct tttgtggctt ttagctggtt gtttgatttt ttaaaggtag caatttacgt
     7081 taaatgaatg gcatgttgtg actggttgta gctgtaactt tgacagaagt ggcataaggt
     7141 agcattaaca gcaaggagga gatggcaaat ggctagctgg ccgatgacag ctccctgtga
     7201 atagccagca gctgattggc atagtcacgg ttgttggata gccgtggcac cttgttctgc
     7261 ccgcccagct tgttgtttcg tttcatccat tcaaaaaagg ttcctttggc cagcgaccga
     7321 accaagggga agtcgagggt gatattcttg tagcgcttgg cttcgtagtc cgagttaacc
     7381 tcgcaaagag cagcatcgag aagcctcgaa aattcatcca ggctggcagg gtcaacgtta
     7441 aactcgataa gccattcgtg gctacccttt ttatcggttt ccatgtaaac cggggctgcg
     7501 gtgtactcgg caacctgtgc cccagttgca tcgcaggctt tcttaattgc tttttcggcg
     7561 ttatcaataa tgatctcctc gccaaaagcg ttgatgaaat gtcgggttcg cccggttatc
     7621 tttattttat agggtgattt tgaggtgaag gttaccgtat cgccaatcag gtatcgccac
     7681 agccctgaac tggtggtaat cagcatggcg taatttttac ccacctcaac ttcaccaatg
     7741 gggtaggccg ggggattggg ctgccccagc tgttcggcag gaataaactc gtagaaaacg
     7801 ccatagtcga gcatcagcag catatcatcg gtttccgggt tgtcctgaat ggcaaaaaaa
     7861 ccctcactgg cgttgtaggc ctccatgtag tgcatctggg gtgagggaat tagcttaaag
     7921 tattggtcgc ggtaaggcgt aaagctaacg ccaccgtgaa tgaatagctc caggtttggc
     7981 caaacctcca gcaggttcga ctttccggtg tggttcagaa tatgcttaat catcaccagg
     8041 ttccatgatg gcacaccggt aatgctggtt acgttctggg ccaaggtttc gccggtaatc
     8101 ttttccagct tctgctccca gtcggggata agggcaaccc gttggctggg agtgcggata
     8161 aaatcggtcc accagggttg gttttgcagg agtatagccg ataaatcgcc gtagcgggag
     8221 ctgcccgaaa gggtatccac ctgatggctt ccgcccaggg taagtccctt gcctctaaaa
     8281 aggccatttt tagggtacag gtcaaagtaa atagctatca catcgcggct tcccctgaag
     8341 tggcatccgt tcaacccctc ccggcttacc ggtataaatt tactcttgtt ctgtgttgtt
     8401 cccgaagatt tggcaaacca gctaatttta ccgggccaaa gcaggttctc ctcaccggat
     8461 ctcatccggt ctatgtaggg tttgaagtct tcatactcca ttacaggtag tccctgttga
     8521 aaatcggcta tggatttgaa cctgctaaat ccgttcagct gtccatactc cgtatttttt
     8581 gccctggaaa gcaggttgaa aagggtttgc tcctgcacct cctgcgggtg ccttcggaag
     8641 tagtcaatgc tgcgtaaacg gctggagtta atccggttga ggagtgaatt caccaaaggc
     8701 atacccttaa agttgttcga tttcctagca aatttaaact aaatctacaa atttccatcc
     8761 ctttgcatag gagaagtaaa caaatgcctt caacaaccgg tatggtacgg ttgtctcccc
     8821 ggaccggcgt tcttctcaat taatatgggt ttataccgta tagaagatgc aaaaaaaaag
     8881 tctgaccagg gtgttgaaca agttctagtt cttcaacccg gtcagaccag agcacaaacc
     8941 aagctatttt cctataagtt ctctctgagt cagctccttt agtgtttcgt acttatgtgc
     9001 tccgcttagc agcttcctgc cgttaatcag cagggaagga acagcattaa cacccagctc
     9061 cttggccctg tatgaatcct ggtaaatggc atcaagggta gcatccgact caaagtcgtt
     9121 cttaaaacgt tccgtatcaa gccccacttc gtcagcacat tgaagcagca cattaaaatc
     9181 ggctatgttt tgtgtttcaa ccaggtgagc cttttgtatc ctgtcgaaca tattccaatg
     9241 cgccaattgc ccaccctgca gctcggctga cttgcaggcg agcaggcctg gcatggagta
     9301 ggggtagtca aatgttctta gcgccatgtc ctcggtgtgt attctatgct catcatcatt
     9361 ctcgttggca tttcgccagt ggttgagaat ttccctcttg gcagcttcct tactgccaaa
     9421 catttctacc agcctatcag catctgatgc aagggcaaaa gagcgatgta taacctcgat
     9481 ttcggggaat tctgccacca gcctccgcat tcgtggggaa agtgcaaagc accaggcaca
     9541 aagaacatca tgaaaaaatt caacctgcat agtggcaact tttcatgtaa gttattttgc
     9601 ggggaagtac ttattgaaga atgttaccat atcgctccac gaagccgaat cggcggctgc
     9661 attgtaggaa atgggcatgt tgaacttttt gccggcttcg gttgaggctt tattggagaa
     9721 ggcatgggtg gcaccctcgt aaaccttaaa ctcgtagtta acccccatat tctgcatttc
     9781 actcttaaag ttgtcaatat cagcctgtgg aacaaaggga tcggcgccgc catggcaaat
     9841 cagcacgttg acgttcttca tctcgggggt agctttaaat ccagttaatc caccatgtat
     9901 gcttaccact gcatccaggg gaacacccat gctagctgag ttaagcacaa cagcaccacc
     9961 aaaacagtag cctattgctg caatacgctc ggcattaacc aggctgtcgg caattagtgt
    10021 gttgtaggcc accaacatgc gctccttcat caaggctgga tttgtgtaaa actgattggc
    10081 agtgttttgg gcatccttgg gattgtcgag tatcaacccg ttgccgtaca tgtcaactgc
    10141 catggctaca taaccaagct cggccagcat gttggcgcgc attctggagt aatcgttgtt
    10201 cccccaccac tcgtggacca ccagtatgcc tggacgcttt ccggttaaac tttcgtcgta
    10261 ggcaatgtag cccttaagtg gggttgagcc gtcgtagtag gtaatctctg ttccctttac
    10321 tacaggttgc atggttggag gagtagaagt ttcacctgct ttgttacttt tattcccggt
    10381 gcaggaaatc agcgctgcaa ttgctattgc aaaaattgat aaggtaattg ctctcttcat
    10441 ggtataatta tttttttcaa acatataaca acgggtacag tcaaaaagtt caactatgct
    10501 tatggcaggg agttacaata gctggaatta ggtggtattt tttagtcatc aggcgaattt
    10561 atgcaagcgc agctcttgca gattatagat attcaggata atagcaggcc aagcatgcac
    10621 gaacggccgc ctcaccaaaa gttatggaag gctaacggac aaaaatgtga tttagagaag
    10681 ttttagccag ggttagattt tttcgtactt ctcagggaat atgcaccaat gatgaggatc
    10741 tgcaaagcac cttgcacgtc gggacgaacc acctgcaacc ctgctaaaat tatgactaca
    10801 ggaaaatgtt tgtattagcc cgaaaaccta tagcttaaca gatagaccaa ctcctaaaat
    10861 ttctttgaat tgaactcgtg gggactttga gtcgaaggta ccatctccgt ttttatcaat
    10921 gttaatttta atcttgtcgt cgtagatgag catggtgctc aggttcatgt tgaggtactt
    10981 gttcacctta attgcaacca gcgcttccca gtttaccact atgttttggg gtttgtcggc
    11041 atagtttgag aaaaaatcaa ccttggtggt gaacgataga ttttttaaaa cctcaggctt
    11101 aaagtcagcc tttgagaagc ttatccgcac gtagccacca aattccgatc tatactcctt
    11161 gcccggtttt accccaaagg caccagtgct cgacaggatg gaatcggtaa caaatgtaaa
    11221 ttttcctgta aacggagcaa cgaaaaggct taggtagctg ctgggtttta tatccatgcc
    11281 aagcgcaccc accaggtatg cgggggcaaa aaagttcgaa atcatgtttc gggtgctatc
    11341 gttggggtag tcataccctt tcgccatttg ggttttaaag ttcagcagca gggcgtagta
    11401 aatatttttt gaggctctat acccgtactt ggtggaaagg tcaactctat cgtcggtttt
    11461 tttattgtac tttaccccat tctggttgag gaacccatag cctatatcga gggtgttatc
    11521 ccatgccacc ttttcattct taaagctgac gaacccgcta agcatggtgg caagcgatag
    11581 ggaattttgt cctcctgccg cccagtttgt cagcgaggtt tgtgagaagt tgattccggt
    11641 tacaaatccc ttcttccagc caaggattga atcctcactt tgagttttca actcctttac
    11701 tacggtttcg gaaatttgtg ctgtaattag gttgctaatc attagaaacg ggatgacaag
    11761 cagaaaatct tttttagtgc tcatggtttc ggttattaca atgacattgt gggtgatttt
    11821 ttaggttgat ttggattttt ttgctccaaa ttttgtttcg aatagccttt gcagggcaaa
    11881 catttcatcg cggtatcggg cggcttcaaa aaagttgagg tctttggctt ccctttccat
    11941 ttcctttctt gcgcgttcaa tggcttcagc caattcgggt tttgtcatgt actgaacaac
    12001 gggatcggcg gcaaagtcca ccgtgtcggg ctcaacgtag ggcttaacca cctcgccggt
    12061 tgaaagtttt ccggccatga ttgatttttt gggcttgaca atttgagtcg gggtaattcc
    12121 atgcagctcg ttgtatttca gctgcttttc gcgacgcctg ttggtctcat ccatggtaag
    12181 ctgcatggag cctgtgattt tatcggcata catgattacc cgtccgttaa ggttacgggc
    12241 agcccgacct gcggtttgag taagcgagcg tgctgagcgc aggaaaccct ccttatcggc
    12301 atccagtatg gctaccagcg aaacctcggg caggtcgagt ccctcccgaa gtaggtttac
    12361 gcctaccagc acgtcaaata gcccttttcg caaatcctcc attatttgaa cccgttccag
    12421 ggtatccaca tcggaatgaa tataccggca acgaatgtcg aacttcacaa ggtactttac
    12481 cagctcttca gccatacgct tggtgagggt ggttaccaaa accctttcgt cgcgttttgc
    12541 actggcattt atttcaccaa tcaagtcgtc aatttggtta aggctaggcc tgacaataat
    12601 aggtggatcc agcaggccgg ttggcctgac cacctggtca accaccaccc cctggcacag
    12661 ccgaagctca tagtctgatg gtgttgcgct tacaaaaatg gtttgcccga ttaagccctc
    12721 gaattcgtcg aatcgaagtg gacggttgtc aatagcggca ggtagcctaa agccatactc
    12781 cacgagcgtt tgcttgcgcg agttatcacc tccatacatc gccctgacct gcgaaagggt
    12841 tacgtggctt tcgtcaatta ttgtcaggaa atcatttgga aagtagtcga gaagacagaa
    12901 aggcctcatc ccggcctgcc ggccatcgaa gtagcgggag tagttttcta taccagggca
    12961 gtagcccaac tcctttatca tttccaggtc gtactccacc cgttgctgaa tccttttggc
    13021 ctcaaccatg tggcctatgc ttctgaagtg ctccgcctgc ctcatcatat cgtcctgaat
    13081 ttgggcaatg gcctggttta tccggctttt acctgcaata aatatattgg cagggaaaat
    13141 taccgcctcc tccatagtct cgatggaaac cccgctaacc ggatcgaaca tttcgagcgc
    13201 ttcgatgtcg tcgccccaga aaataaccct aatgcccttg tctccgtagg caaggtatat
    13261 gtccacgcta tcgcccctga cacggaaggt gcccctttta aactccacct cgtttcgggc
    13321 gtaaagccca tcaaccagct gtcgtagtag ctggttacgg ccaaggtgct gcccaacttt
    13381 aacccgaacg gttccgttgt agaagtcctg tggatttcca attccgtaga ggcatgaaac
    13441 ggaggaaacc actattacat ccctacggcc tgataggagg gaagaggtgg cgcttagccg
    13501 cagcttttca atctcatcgt ttatggatag gtccttctca atgtaggtgt cggtaacagg
    13561 taggtaggcc tcaggctggt agtagtcata gtacgaaaca aaatactcaa cagcattttc
    13621 ggggaaaaaa cttttgaact ccccgtaaag ctgagctgcc agcgttttgt tgtggcttag
    13681 caccagcaca ggcctgttta gctgggcaat aacgttggca atggtgaacg tttttcccga
    13741 accggtaact cctaaaaggg tctgaaactt atcgccccgg cccagcccat cggtcagctg
    13801 cttaatggcc tccggttgat cgcccgatgg ttgaaaatcg gaaacaagct tgaagttcat
    13861 accttagtgt gcgagttggt gcatcgatta ctgacatctg cagccagctt atcaaaaaca
    13921 ttataaatcc gaaaaaagct tccaggatgt ctaaaaagtt agtccagatt atttttctgc
    13981 aaaattagct attttaacgg cttgttggaa gtactgggac aggtagccgg ttagaacctg
    14041 acgggtagcc tgaatgtgaa aaagtttaca aggtggatgt agccggtgcc taaaattgct
    14101 taccttattt atgctgcgga attgggcggg caatatgaat gttgtttaat tcggcttacg
    14161 gatcagtata accagtgtga gcataagcca ttgctattga tggaaatgcc ggtaggaaga
    14221 agctattagt tttcagaaaa caggtcgttt accttgacga tcggatttgt atggctaaga
    14281 tacttattca tggtttccat cagtttgtta atgttaattg gtttagggat gtagtcgtta
    14341 caccctgcct gaaggcattt ctccttatcg ccggcaatga cgcaagccgt ttgggcaata
    14401 attgggacat gcgggtcgcg ctctcggatt aaccgggttg cgtccagccc attaaggcct
    14461 tccatcttga tgtccatgag aattaagtct atgccgtgag tgctgagaaa ggtatccaca
    14521 gcacttctcc cgttagagac aaaaatcatc tttgcccctg ttttagaaat tataagctca
    14581 agcagcattg agctggcacg atcatcatca acaataagta tcaacctgtt actccagttg
    14641 tactgctcaa gcgtggtcat ttaggaagtt gtaagtagct ttaaattgaa agcaagttat
    14701 agctttttac aggtttatat caactgaaac atgcttaaaa attacgaaaa tgggggattg
    14761 ccttatgaag ctagtaattt gggagactag tactaaaatt gagtttttaa aggaatagcg
    14821 tttacctggt ttcgagaatt ttcagaatcg agtttgttcc gggtacccgg taagttttat
    14881 cgcccttttg cacaatgcat tgcttgttac ggcggtctat tcccgccagg tattttaggt
    14941 tgataatggc cgaacggctt acccgtagaa atggtaaccc ctccatttgc gattccacgt
    15001 ctttgagcga aacagaaacc acctcgtcct ttcgggaatg aaagtggagg atggtgtagc
    15061 tgccacatgc ctcgcaccag gtaatctcct cgggttcgat gaatataatt cccctgcgca
    15121 tgctgaactt cagcttcctg aactcgggaa ggcagctaag cagcttatcg gctttggcct
    15181 tcatcgttcc cctgtcgatg ttcactttgt agcgatcgag ggtgtcaacg agatcgtcta
    15241 aatctattgg ttttaggagg tagtcgaaag ccgcatactt tatagccttt atggcaaact
    15301 ggttgtaggc agtaatgaat ataaccgcag ggttataggg agtcttacgc aacctttcga
    15361 gcagctcgaa ccctgaacca cccggcatct caatatccag aaatagaata tcggggttgt
    15421 gctccctgac tgcctgcatg gcctggtcaa tattctcgga tgctcctaac acttgaatat
    15481 ccttgtcgaa cgaaagcatc tgaaccagga tgtccctgga gttggcctcg tcatcaacaa
    15541 caaaagcagt tatagttttg cccattatac ttaggtatat tccaaatttc aaagccgagt
    15601 gatccagctc attttttacc cgtgcaaaat tttattgcta taaccccgta agctactatt
    15661 ttgttatatg cactcgttta tgttccgatg aacccccaaa gggtttgccg taagcaaacc
    15721 tccattaacc ggcttcgctg aactcgcata aaacgcttgg tagtttattc gctcttgtac
    15781 aagccatcta catgggcgtt tcataattat gcaatcctgc tgccacaaaa tttttgaggt
    15841 aagctaccat caacttcaaa agtagctatt atttgataat tttctccaac aagccatctt
    15901 aaattcatcc tggcaaccat gttgctggcg taaggggttg acagccaagc atgttcaccg
    15961 gtgccgggtc atacaaaccc accaaatatt tttggctgag caacccgagt tacaggggtc
    16021 ttcaattttt tagttgcaca gcttgacgtt aatggcaata tgactagttc aatttaaaaa
    16081 aatgcgaaaa gtaaggagtt gaataagaag tatataatgt tgatataaaa gcaaaagcgc
    16141 tcaaatcaaa ataatgggtt tgctgt
//
LOCUS       DAOSHM010000053        15896 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_3025_length_15896_cov_20.071018, whole genome shotgun
            sequence.
ACCESSION   DAOSHM010000053 DAOSHM010000000
VERSION     DAOSHM010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 15896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15896
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3025_length_15896_cov_20.071018"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1003)
                     /locus_tag="PK839_08265"
     CDS             complement(<1..1003)
                     /locus_tag="PK839_08265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72766.1"
                     /translation="MNFSRSIKSTSITKRSIPLLTTLLIFTFTLGHSQIITTGLPVMK
                     HFRKTDYRAGTQNWGISQDKQGFMYFANNNGLLRFDGNSWDLFPIPGNSLVRSVLCIN
                     DTIYVGAFEEFGYYTYSNGNDLTYTSLTQKIAGKAWGIDEVWRIYKYNNTILFQTFTY
                     IMVLEKGEISAFPTPEVLQFSFLIGNDFFVQGKNGKIYKFLQNRFEPIDDGGKFAGKQ
                     IWAVLKQENGSTLLATINHGVWEYNNGTVKAWNNAANSYLIKNQIFTASTLRGGYIAF
                     GTIQAGLVVTDEKGEIVLLIDKKRGLQNNTILSLFTDLHNNLWLGLDNGIDYMELNSP
                     "
     gene            1320..2843
                     /gene="gpmI"
                     /locus_tag="PK839_08270"
     CDS             1320..2843
                     /gene="gpmI"
                     /locus_tag="PK839_08270"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HOG72767.1"
                     /translation="MKHGKVLLMILDGWGIGNKDKSDVIYSTPTPNIDTLTAAYPNAQ
                     LLACGENVGLPDGQMGNSEVGHLNIGAGRIVYQDLVRINKDIKENKLSKMPVLVEALS
                     YAKSNNVSVHFIGLVSDGGVHSLDKHLYNLLDITKEYGLSRVFIHALTDGRDTDPQSG
                     LGFIRNLENHIKTSNGIIASLVGRYYTMDRDKRWERIREGYDLMVHGKGKPTTNMAKA
                     VEESYREGVTDEFIKPIVRVDENGNPVGLIREGDVVICFNFRTDRLREITIALTQKDM
                     PDFGMKTIPLHYVTLTRYDDTFKNVNAIYEKDNLINTLGEVVSVNGLKQLRIAETEKY
                     AHVTFFFSGGREQEFEGESRILIPSPKVATYDLQPEMSAFLVKDAIVEELKKQTHDFI
                     CLNFANGDMVGHTGVYTAIQKAVQTVDTCAGEVVSTARANGYDVIIIADHGNADYALN
                     PDGSPNTAHSLNPVPIIMVSDKFKAVDSGVLADVAPTILTLMGLTIPPEMTGRVLCH"
     gene            2861..3442
                     /locus_tag="PK839_08275"
     CDS             2861..3442
                     /locus_tag="PK839_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3109 family protein"
                     /protein_id="HOG72768.1"
                     /translation="MIQIDDKVVSSEILTQRFCCEIASCKGLCCVQGVSGAPLTQQEA
                     DLLDILLPAIKPFLSAKGMQTIEGHGVSVVDLDGDLVTPLVEDKECAFAIFENGAACC
                     AIEKAWRNKSIDFQKPISCHLYPIRVKQYATFTALNYDVWKVCDPARLLGDKLGLPVF
                     RFLRDAIERAYGKEFYNQLEEASKLLSARQPDS"
     gene            3473..4843
                     /locus_tag="PK839_08280"
     CDS             3473..4843
                     /locus_tag="PK839_08280"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HOG72769.1"
                     /translation="MDTIKSYVEANKNRFLEELFELLRIPSISTLPEHKADMLKAAEY
                     WKKTLIEAGSDRAVVMPTAGNPVVYAEKIINPALPTVLVYAHYDVMPVDPLEQWISDP
                     FEPQIRDGKIWARGANDDKGQSFIHAKAFELMVKTNSLPCNVKFMIEGEEEVGSVSLG
                     KWCADNKELLKSDIILISDTSMMGPDTPSITVGLRGLAYVEVEVAGPSNDLHSGLFGG
                     AVANPAIILAKMIASLHDENNRITIPGFYDDVLEVSAEERAAMARAPFNLQGYKNSLD
                     IDDVHGEVGFSTIERTGIRPSLDVNGMWSGYTGNGAKTVLPSKAFAKISMRLVPNQDY
                     NKVALLFKDYFESLAPKSVKVKVNIHHGGQAYMAPITTPAYLAASLAIEDVYGKKPIP
                     FRSGGSIPIVSTFEQILGVKSLLIGFGLESDAIHSPNENFSLHQFFNGIDTIARFYGY
                     FGGHNV"
     gene            4964..5953
                     /locus_tag="PK839_08285"
     CDS             4964..5953
                     /locus_tag="PK839_08285"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF002378.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HOG72770.1"
                     /translation="MQKKLNIAIMAGGNSSEENISLMGASQVEGWLDPGKYNPYKVLV
                     KGTDWTCMVGNGVAVPVSKDDFSVSLNGVRVTFDCALIVIHGNPGENGLLQGYFEMLN
                     IPYTTGGVLNEAITFNKHFCKLLLTGTGVKLAREVLVHRGEEIDVDAVAGKLGLPVFV
                     KPNASGSSYGVSKVKHPDEILPALNRAFSEDSTVIIEEFIAGRELTCGAFKSFNREFI
                     LPVTEIVPEKEFFDYEAKYLNKSKEITPAPIPEDLYGTIQGLTSKIYDVLECRGIVRV
                     DYIAGANGIYFLEVNTVPGMSEASIVPQQVRVMGENMRDMLTLVIEDALRRAK"
     gene            complement(5955..6170)
                     /locus_tag="PK839_08290"
     CDS             complement(5955..6170)
                     /locus_tag="PK839_08290"
                     /inference="COORDINATES: protein motif:HMM:NF020917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HOG72771.1"
                     /translation="MIESAKHAASLHFLKHGPVRVEGTFRIVDIDGNELEVAEAGKVY
                     LCTCGKSNTKPFCDCSHQEELKLRATN"
     gene            complement(6173..6664)
                     /locus_tag="PK839_08295"
     CDS             complement(6173..6664)
                     /locus_tag="PK839_08295"
                     /inference="COORDINATES: protein motif:HMM:NF018595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HOG72772.1"
                     /translation="MGNIRKKYSNDEITVYWQPAECIHSTICFTRLASVFNPRKRPWI
                     DMSGGTTDQILKIVNECPTNALTFSWLENSRNEHETSPKVVREIKPTSEAGTENVKPV
                     KIQVMPNGPLLLSGDFQLFDADGVELKSMKMVSVCRCGHSQGKPFCDGMHFKIGFRDK
                     EEQ"
     gene            6903..9095
                     /locus_tag="PK839_08300"
     CDS             6903..9095
                     /locus_tag="PK839_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HOG72773.1"
                     /translation="MSKLRFSAFKEAVNRPVVDANGTYSAKQSEIFGINVFDREKMKN
                     FLSAEAYESMITSIDEGKHIDRKIAGQVAAAMKAWAIEHGATHYTHWFHPLNGSTAEK
                     HDAFLSVSYDGKPLEVFNGDMLVQQEPDASSFPNGGIRNTFEARGYTAWDPSSAAFIM
                     EHTLCIPTIFVSYTGEALDYKTPLLKSLALLDRAATSVLEYFDRNAKKVFVTLGWEQE
                     YFLIDEALYNARPDLALCGRTLMGHAAAKDQQLEDHYFGAIPARVSAFMRHFEFEACK
                     LGIPVKTRHNEVAPNQFECAPIFEEANLAVDHNQLIMSLMDRIARQHGFRVLFHEKPF
                     KGINGSGKHSNWSMMTDTGINLLSPGKTPKNNLQFLTFLINTIKAVHDYPDLLIGSIS
                     SESNSHRLGAHEAPPAIMSVFIGETLTRVLDELEERVSDKKMTPDEKTELKLDIIGKI
                     PEILLDNTDRNRTSPFAFTGNRFEFRAVGSSGSCASPLIVLNTAVANQLNQFKKEVDE
                     AIERGIKKDEAIMQSLRRAIIESRKILFEGNGYSNEWVEEAAKRGLPNIPNPIEAFSI
                     FLHNSSQKLLSGNNVLSVKELEARHEIFNETLMKKVQIEARILGDLAINHIIPTAISY
                     QNTLISNVKGLKELYPDEWEKLAVHQIGLIKEISEHISEISNQVNDMVERRKEANRVD
                     DVVERARLYAQNVKPFIESIRVHIDKLEMIVDDEMWPLPKYREMLFIR"
     gene            9364..11364
                     /locus_tag="PK839_08305"
     CDS             9364..11364
                     /locus_tag="PK839_08305"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOG72774.1"
                     /translation="MNKLTLTRRIALLACFAIAANISFAQTSKLAKADALYKAGAYFE
                     AIDLYKADIDKINDKNELPKYLYKLGNCYRLTGNARQAELWYQKAIMREYPDPKVYFY
                     YAEMLKMGEKYNEAIEQYQKYKVAVPLDPLADAGIKSCELSIKWKETPSGYEITNMRS
                     LNTRTSEFSPAYASSDYATLFFTSSRNEATGGKVSSVTGENFTDIFISERDNKNVWSK
                     PRPIDETINTSYDEGTCALTSDYNTLFFTRCKVSKRDKLGCQILVSKRAEQGWLTPDE
                     LKLAADSMIVAHPSISADGTELYFVSNISGGIGGLDIWKITRSSSTSDDWGEPVNLGG
                     EVNSAGNEMFPYIHPDGTLYFSSDGHPGMGGLDIFRSWVNDEGRREVENMRYPINSSA
                     DDFGIVFEKDREAGFFSSRRQGGRGADDIYMFYLPPINFNLIGHVTDEKSKKPVEQAT
                     VKLVGNDGSVVTMQTLADGSFRFNLTPNADYVVIASKKGYLNNKAKESTRGLTQSKDF
                     EVSIALTSTEKPIEIPNIFYDYDRWELRPESREALDRLVEILNDNPNIIIELASHTDS
                     RGTKEYNYELSQKRAQSVVNYLIEKGIEPVRLKAKGYAQTQPKVVDDALASRYPFLQV
                     GTVLDQTFIDTLTSDEQKEIVYQINRRTEFRVLKEDYSAKPK"
     gene            11471..12676
                     /locus_tag="PK839_08310"
     CDS             11471..12676
                     /locus_tag="PK839_08310"
                     /inference="COORDINATES: protein motif:HMM:NF040149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72775.1"
                     /translation="MKVATYHLLAAFLLPAFAAGQIAESRVTFDISAAEAFIGTFEAM
                     QQGKSEKQVDSLIDGMLALHAYAVSAERFGAKNQISPVTLGEYKRFLKSFISDSVDVQ
                     GNTRLGMLKSRYVDTYSNLPDYKKLVNQLKAQLNEYSSGAVQLATEWLPKNVGLNAEV
                     LVMFDIGGAAWAYKASDGKDYIMYSVLFLMDDSRNFDSDMFFKNLAHELHHVGIPLNS
                     FANEVGYDSLPPNDRLKLYIDFMYDIVKEGMAQKFCSNAPGKFTPKPYPNEIYAAIPK
                     GVENWNYFISDFQAINDSVKSFLNFIAYSDSVDAGQFYNRFVRYWTWKAGEVEKKEFA
                     LDRRYYYGAEVLGLVYTTLGKEALFEIMYDYRKLPSYFNLALKKAKPEGYESFLFDDR
                     LADSVLHLK"
     gene            complement(12750..14684)
                     /locus_tag="PK839_08315"
     CDS             complement(12750..14684)
                     /locus_tag="PK839_08315"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic peptidoglycan transglycosylase"
                     /protein_id="HOG72776.1"
                     /translation="MQRRQRVTAIITATIGLILVLYGLFRNRMLSYVFEQKTERLTTA
                     YNLQVAADRLKFRGIRTLDISNLVVLNSHADTLAQVGAARIKLGLLHLLVLKVNPLAI
                     ELKDSWFNLNTLLERNSIQNKEAITAEANKQEKFNLLPIYKVIKGTFGLSTAKINIDN
                     LTISYIDSLHYETATVTSWNYKNSRFSASVATQHGDRRGSFELAGESSKSRNSFRVWV
                     YGRSFQVEIPFIEALTGVGVYLDSAKLDFRAGELHGNLMALNIETMAKNISMEGSRIA
                     PYPMNIDSASLKLDIKLKPSEICLDSTSTLSLNRLSTSFKSCYTLDKSPTLTLKFKTA
                     TTRWQDLVESLPSGLFTHIAGMKLRGTFSYTLKARVNPESPDSLVIEPLLSSSGFGIS
                     SFGQTNFYALNDTFTHRVYKDGRWIRNIRVSSDNPEFTPLNHISPFLKWAIITSEDGN
                     FYTHGGFDLDGIRYALACNIRERRFARGGSTITQQLIKNLYLNQEKNIARKAEEMLMV
                     WIIENTAAVSKDRMLEIYLNIIEWGPDVYGIAEAASYYFNKKPNELTLQESLFLAYIV
                     PRPTKFKYLFDDQDRLKPFLYQNFTDVASKMLMRGYITENDFQAIDSTALQLPLHGRA
                     LELIAVEPDLADSTQLVEVE"
     gene            complement(14771..>15896)
                     /locus_tag="PK839_08320"
     CDS             complement(14771..>15896)
                     /locus_tag="PK839_08320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72777.1"
                     /translation="YDAILDTYKSLDARDGELIFYPLRPRLDSTGKDINKAVQLQMLS
                     ISTRLGLSVRGPDILGAKSSALVEADFYATKNDYVSLLRIRHAMFKLKWATTELLVGQ
                     YWHPVFVTDVAPSVISFGSGAPFHSLNRSPQIRFSYFPYPMVRISLTALMQSYHKSSG
                     PEDAQRNSGKPELMLQAAAGSAEEFLAGLSAGYKWLTPRLVTQTGYATCKTSGQYLFS
                     AFLLKKLGKTVVKAEAVYGQNPTHLNMIGGYGMASGSGPAPNDDYDYTSLNTLSTWVD
                     VQHQAGKMGVGLFAGYSKLYGADKNYTSLAGYSRNDDLNNIYRISPRLSYQAESITFA
                     LEYMLTAATYGSTFDNRHRVTSSLDPVYNHRATLNIKYSF"
BASE COUNT         4196 a         3624 c         3815 g         4261 t
ORIGIN      
        1 tgggcgagtt cagctccatg tagtcaatgc cattatctag tcctagccat aagttgttgt
       61 gcaggtcggt gaaaagactt aaaatggtgt tattttgaag ccctctcttc ttgtcaatta
      121 gtagaacaat ctctcccttt tcgtcggtta ccaccaaacc cgcctgtatg gttccaaaag
      181 ctatgtatcc cccgcgcagc gttgaagctg tgaaaatttg atttttaatt aaatagctat
      241 ttgcagcatt gttccatgcc ttaacagtcc cattgttata ctcccatacg ccgtggttta
      301 ttgttgccag caaggttgaa ccattttctt gttttagtac tgcccaaatc tgctttccgg
      361 caaattttcc accatcgtct attggctcaa atctattttg caggaattta tagattttac
      421 cattcttccc ctgaacaaaa aaatcgttcc cgatgaggaa tgaaaactgg agcacttcgg
      481 gagtgggaaa agcgcttatc tcaccctttt cgagtaccat gatataggta aatgtttgaa
      541 aaagaatggt gttgttgtac ttgtaaattc tccaaacttc atctattccc cacgcctttc
      601 ccgcaatttt ttgggtgagc gaagtatagg taaggtcgtt gccattactg taggtgtagt
      661 agccaaattc ctcgaatgcc cctacataaa tggtgtcgtt tatacacagc accgatctaa
      721 ccaggctgtt tccggggatg gggaaaagat cccagctatt cccgtcaaac cggagcaggc
      781 cattgttatt ggcaaaatac ataaaacctt gtttatcctg cgaaataccc cagttttgcg
      841 tgcctgccct gtagtcagtt tttcggaaat gcttcattac aggcaatcct gtggtaatta
      901 tctgtgaatg gcctagagta aaagtgaata ttaataatgt ggtaagcaga gggatgctcc
      961 ttttggttat gctggttgat ttaatactcc tagaaaagtt catttttatg ggctatgttt
     1021 ctaatggact ttggtttata tgctccgttc gatttttaaa tctggcaata acaacctttc
     1081 atcatttggg ataaaattat taaaaaatta ctacgaacgg ttaatttttt aacaaatagc
     1141 agggtaagct gataggtgtc agtacaattt tcaaacgttt acgccacaac cggtcaataa
     1201 taagccaaca ttagccgaag gcaattaaaa aagggtaact ttgtagtcgt gtacgaacat
     1261 aaaggttact gaaaacgttc ctggtgtgtg gttttaaatg aaaatataac ttacaggata
     1321 tgaaacacgg aaaggtttta ctgatgattc ttgatggctg gggtattgga aataaggata
     1381 aatcggacgt aatatacagc accccaacgc caaatattga taccctaacg gcagcttacc
     1441 caaacgccca gctcctggca tgtggtgaaa acgttggcct acctgatgga caaatgggaa
     1501 attcggaggt tggacacctg aacattggcg ctggtagaat agtttaccag gatctggtgc
     1561 ggataaacaa ggatattaag gaaaacaagc tgagcaaaat gcccgtgctg gttgaggctc
     1621 ttagctacgc caagagtaat aatgttagcg ttcactttat tggtctggtt tccgatggcg
     1681 gagttcactc actcgataag catttgtata acctgcttga tattactaag gagtatggcc
     1741 tgtcaagagt attcattcat gctctgaccg atggccgcga tacagaccca caaagcggtc
     1801 tgggctttat cagaaatctc gagaaccata taaaaactag caatgggatt atcgcctcgc
     1861 tggttggtcg ctactacacc atggacaggg acaagcgttg ggaacgcatt cgcgagggtt
     1921 acgacctgat ggttcatgga aagggcaagc ccacgaccaa catggctaag gctgttgaag
     1981 aatcataccg ggagggcgta accgatgagt ttatcaagcc cattgtcagg gttgacgaga
     2041 acggaaaccc tgtcggcctt attcgcgaag gcgatgtagt tatctgcttt aacttccgca
     2101 ccgaccgtct gcgcgagatt actattgccc ttactcaaaa ggatatgccc gatttcggaa
     2161 tgaaaaccat tccgcttcac tacgttaccc ttacccgcta cgacgacact tttaaaaatg
     2221 tgaacgctat ttacgagaag gataacctca tcaacacgtt gggcgaggtt gtttctgtta
     2281 atggcctgaa gcagctacgc attgccgaaa ccgaaaaata tgcccacgtt accttctttt
     2341 tctcgggtgg gagggagcag gagtttgaag gcgaaagccg aatacttatc ccttcgccca
     2401 aggtggctac ctacgatttg caacccgaaa tgagtgcgtt tcttgttaag gatgccattg
     2461 tggaggagct taaaaagcaa acccatgact ttatttgcct taactttgct aatggcgaca
     2521 tggtggggca cactggcgtt tacacggcca tccaaaaggc cgttcaaacg gttgatactt
     2581 gtgccgggga ggttgttagc acggccaggg ctaacggata tgacgtaatt attattgccg
     2641 accatggtaa cgccgactat gctcttaatc ccgatggttc gccaaacaca gcccattcgc
     2701 ttaatcccgt ccccattatc atggtttccg ataagttcaa agctgttgat agcggtgtcc
     2761 tggccgatgt ggcacccacc attcttaccc tgatggggct gactattcct ccggaaatga
     2821 ccggaagggt tttgtgccac tagcacaccc cgccaacatg atgattcaga ttgacgacaa
     2881 ggtggtcagc agcgaaatac ttacccaaag gttttgctgc gaaattgctt cctgcaaggg
     2941 tttatgctgt gtccaagggg tatcgggcgc acctcttacc caacaggagg ctgacttact
     3001 tgacattttg ctcccagcca ttaagccatt tctttcagca aagggcatgc aaaccattga
     3061 agggcatgga gtctctgtgg ttgatttgga tggcgacctg gttactcctc tagttgaaga
     3121 caaggaatgc gcctttgcta tttttgaaaa tggagccgca tgttgtgcca ttgagaaggc
     3181 atggaggaat aaaagcattg attttcagaa gcctatttcg tgccatttgt acccaatcag
     3241 ggtaaaacag tacgccacgt ttaccgcact taactacgat gtctggaagg tatgtgaccc
     3301 cgcacgcttg ttgggcgata agttagggct gcctgttttc cgttttttaa gagatgctat
     3361 tgagcgtgcc tacggaaagg agttctacaa ccagcttgaa gaagcaagca agctgctttc
     3421 tgcccggcaa cccgacagct aaaattatta aaaaccaata cttttacctg ccatggatac
     3481 cataaagtcg tatgttgaag ccaataaaaa tcgctttttg gaagaactgt tcgagcttct
     3541 tcgcattcca tccattagca ccctgccgga gcacaaagcc gacatgctta aagctgctga
     3601 gtactggaaa aaaacactta tagaagctgg ttctgatagg gctgtggtta tgcctaccgc
     3661 aggcaatcct gtggtttatg ctgaaaaaat tattaaccca gctcttccca cagttttggt
     3721 ttacgcccac tacgacgtaa tgcccgttga cccgttagag caatggattt cagacccttt
     3781 tgaaccccag attcgtgatg gtaaaatctg ggcacggggt gccaacgacg acaagggaca
     3841 atcgttcatt catgccaaag cttttgaact tatggtaaaa accaactccc ttccctgcaa
     3901 tgtgaaattt atgatcgaag gggaggagga ggttggctcg gttagcctcg ggaagtggtg
     3961 cgccgataac aaggagctgc ttaaatccga tatcatttta atttcagaca cctcaatgat
     4021 gggtcccgat accccctcaa tcaccgttgg actaaggggc ttagcttatg ttgaggttga
     4081 ggtagccgga cccagtaacg acttgcattc cggacttttt ggtggagcag tagctaaccc
     4141 tgccattatt ttggccaaaa tgattgcttc gctccatgac gaaaacaacc gaattacaat
     4201 acccggtttt tacgatgatg tgctggaggt aagcgcagaa gagagggcag caatggccag
     4261 ggctcccttc aaccttcagg gctataaaaa ctcacttgac attgacgatg ttcatggcga
     4321 agttggattt tccaccattg aacgaacagg aattcgcccc tcattagacg tgaacggtat
     4381 gtggagcggc tatacgggca atggcgctaa aaccgttctg ccatcaaaag cttttgctaa
     4441 aatttctatg cgtcttgtcc ctaaccagga ctacaataaa gttgccttgc tttttaagga
     4501 ttactttgaa tccctggcgc ccaaatccgt taaggtgaaa gtaaatatcc atcatggtgg
     4561 acaggcctat atggctccaa tcacaacacc cgcttacctt gctgccagcc tggcaattga
     4621 ggatgtatat ggcaaaaagc ccattccttt cagaagtggg ggaagtattc caattgtcag
     4681 cacttttgaa cagattttgg gcgtaaaatc cctactcatc ggctttggtc ttgaatcgga
     4741 tgccattcat tcccctaacg aaaatttttc gctccaccag tttttcaatg gtatcgatac
     4801 gattgcccgc ttttacggct actttggtgg gcataacgtt tagctaatcg ctgctgatac
     4861 ctgcacttgc aaacagtttc atatttaggg tagaaaaatt ttcaaaaggg gaacaccaat
     4921 ttttgcaaat tttcttgcag ctttgcagct taattttact gccatgcaaa aaaaattgaa
     4981 tatagccatt atggctgggg gcaactcatc agaggaaaat atttcactta tgggcgcctc
     5041 gcaggtggag ggttggctcg accctggaaa gtacaatcct tataaggttc tggttaaggg
     5101 aaccgattgg acgtgcatgg ttggtaatgg tgttgctgtt ccggtttcga aggatgattt
     5161 tagtgtttca ttgaatggcg ttagggtaac ttttgactgt gcactaattg ttattcatgg
     5221 aaaccccggg gagaacggct tacttcaggg atactttgaa atgctcaaca taccctacac
     5281 aacgggtggt gtgctcaacg aggctattac cttcaacaag cacttctgca agttgctcct
     5341 cacaggtaca ggggtaaagc tggcaagaga ggttttggtt catcggggag aagaaattga
     5401 tgttgatgct gtagctggta aactcgggtt gcctgttttt gtgaagccca acgccagcgg
     5461 tagcagctac ggggttagca aggtgaagca cccggacgaa attctgcctg ctctcaatag
     5521 ggcattttcc gaggatagca ctgtcattat tgaggagttt attgccggta gggagctgac
     5581 ctgtggtgct tttaagtcgt ttaacaggga gttcatactg cccgttaccg agattgttcc
     5641 cgagaaggag tttttcgact atgaagccaa gtatcttaac aaatccaaag agattactcc
     5701 cgctcctatt cctgaagacc tgtatggaac catacagggg ctaacctcta aaatatatga
     5761 cgttttggaa tgtcggggaa ttgtcagggt tgactatatt gcgggagcaa atggcattta
     5821 ctttctggag gtaaacaccg tcccgggtat gagcgaagct agcatcgtgc cccagcaggt
     5881 gagggtaatg ggtgaaaata tgcgcgatat gctaacgctg gttatcgagg atgccctgcg
     5941 aagggcaaaa tagtttagtt agtagctctt agcttaagtt cttcctgatg gctgcaatcg
     6001 caaaagggct tggtgttcga ttttccgcag gtgcaaaggt aaactttacc ggcttcggca
     6061 acctccagct cgtttccgtc aatgtcaact atcctgaatg tcccctcaac cctcaccggg
     6121 ccatgcttta agaaatgcag cgatgctgca tgttttgccg attcgatcat tgttattgct
     6181 cctccttatc cctaaatcca atcttgaaat gcatgccgtc gcagaatggc tttccctgcg
     6241 agtgcccaca tcggcacacc gataccattt tcatgctttt cagctcgaca ccatcagcat
     6301 cgaatagctg aaaatctccg gatagaagca acggcccatt gggcattacc tgaattttca
     6361 ccggtttaac attctcagtg cctgcttcac ttgttggctt aatctccctg accaccttgg
     6421 gagaggtttc atgctcgttt ctgctgtttt cgagccagct gaaggtgagg gcgtttgtcg
     6481 gacactcatt tacaatttta agtatctgat cggtggttcc cccgctcatg tcaatccatg
     6541 gccgtttacg gggattgaaa accgatgcca gcctcgtgaa acaaattgtt gaatgtatgc
     6601 actctgccgg ttgccagtaa acagttatct catcgttgct atacttcttc ctgatgtttc
     6661 ccatgatgaa gtggctgaag ggtatttttt tacaaacgta gcgaattctg taaatgttca
     6721 gctgctaaat taaaattttt tagtataaag gccatagaaa cacaaattac gaattttaca
     6781 cttataccct taaaaaaaat gggtaaataa aaatatttat acaaataatt taataaatga
     6841 tattagtatt tgagggtgtg tgttaaaata agtatatatt tgcagcatca aactaaaatc
     6901 tcatgtcaaa attacgattc tcggcattca aagaggcagt aaatcgtccg gtagttgatg
     6961 ctaacggtac gtatagtgcc aagcagtctg aaattttcgg aataaatgtc ttcgacaggg
     7021 aaaagatgaa aaatttcctg tcggcagaag cctatgagag catgattacc tcaatagatg
     7081 agggcaagca tatagaccgg aaaattgcag ggcaggtggc tgcagccatg aaggcatggg
     7141 ctatagagca tggtgcaaca cactacaccc actggtttca cccgctgaat ggctctacag
     7201 ccgaaaagca cgatgctttt ctttcggtaa gctacgacgg gaagccactt gaggtattca
     7261 atggtgacat gctggtgcag caggagcccg atgcgtccag ctttccgaac ggaggaatta
     7321 gaaacacctt tgaagccagg ggctacacag catgggaccc ttcttctgca gcgttcatca
     7381 tggagcatac gctttgcata cctaccatat ttgtatccta cacaggagag gcactggatt
     7441 acaaaacacc cctgctgaag tcgctggccc tactcgatag ggcagctacc tccgttctgg
     7501 agtacttcga ccggaatgct aaaaaggttt tcgttaccct ggggtgggag caggaatact
     7561 tcttgattga cgaggcgctc tacaatgccc gtcccgatct tgctctttgc ggaagaaccc
     7621 tgatgggcca tgctgcagca aaggatcagc agctggagga tcactacttt ggcgccattc
     7681 cggcacgggt atctgctttt atgcgccatt tcgaatttga agcctgtaag cttggaatcc
     7741 cggtgaaaac ccgtcacaat gaagtggcac ccaaccagtt tgaatgcgcc ccaattttcg
     7801 aggaggccaa cctggctgtg gaccacaacc agctcatcat gtcgcttatg gacagaattg
     7861 cccgccaaca cggatttaga gtacttttcc atgaaaaacc ttttaagggt attaacggat
     7921 caggcaagca tagcaactgg tccatgatga ccgacacggg gattaacctg ctatcgcccg
     7981 gtaaaacacc gaaaaacaac ctacagttcc tcacttttct tattaacact attaaagcag
     8041 tccacgacta ccctgatcta ctgattggaa gcatttcgtc ggagtcgaac tcgcataggc
     8101 tgggcgccca cgaagctcca cccgccatta tgtcggtttt tattggcgaa acgcttaccc
     8161 gcgtgctcga cgagctggag gagcgggtta gcgataaaaa aatgacccct gatgaaaaaa
     8221 cggagcttaa actcgacatt atagggaaaa ttccggaaat tctgctcgac aacaccgacc
     8281 gcaaccgcac ctcacctttt gcctttaccg gcaaccgctt cgaattccgt gctgtgggtt
     8341 cctccggaag ctgcgcatcg cccctgattg tgttgaatac tgccgttgcc aaccagctta
     8401 accagtttaa aaaggaggtt gacgaggcga ttgagcgagg cataaaaaag gatgaggcca
     8461 ttatgcagag cctgcgaagg gcaattatcg agtcacgaaa aattctcttc gagggcaacg
     8521 gctatagcaa tgaatgggtc gaagaggctg ctaaacgagg gttgcccaac attcccaacc
     8581 caattgaggc cttcagtata tttcttcaca attcgtccca aaagcttctc tccggcaaca
     8641 atgtcctttc tgtgaaggag ctggaagccc ggcatgagat ttttaacgaa accctgatga
     8701 agaaggtgca aattgaagcc cgtattctgg gcgatttggc cattaaccat atcatcccaa
     8761 cagctatttc ttatcagaac acgctgatta gcaatgtaaa agggctaaaa gaactttacc
     8821 ccgacgaatg ggagaagctg gccgtgcacc aaataggcct tattaaggaa atctccgagc
     8881 atattagcga gataagcaac caggttaacg atatggttga acgtcgcaag gaggctaacc
     8941 gcgttgacga tgtggttgaa cgggctcgtt tatacgctca aaacgtcaag cccttcatcg
     9001 aaagcatacg cgttcacatt gataagctgg agatgattgt ggatgacgag atgtggcctc
     9061 tgcccaagta cagggaaatg ctctttataa ggtaattgaa acgccctgtg gttttggttt
     9121 tggatgtttt ggttaaattt tgcaatttct tgtaggtttg catagctcct gcattacatt
     9181 aaacgtcgca ataatccatt atagaacgaa gcagttaacc aacagcttcg ttctgttttt
     9241 tatcacccta tcataacttt ttcctgttcc gttgatattt gaaatcgtgg tgagctaatt
     9301 catggtggaa taaatatttt gaatattttt gtattcaaca ttaccctact tgaaggaata
     9361 gccatgaata aattaacctt aacccgacgg atagcccttt tggcatgttt tgctatagct
     9421 gcaaatatat cgtttgccca aaccagcaag ctggctaaag ccgatgccct gtataaggcc
     9481 ggagcctatt ttgaagcaat agatctgtac aaggctgata tcgataagat taacgataag
     9541 aatgagttac caaagtatct ttacaagctg ggcaactgct accggctcac cggtaacgcc
     9601 cgacaggccg aactctggta tcaaaaagcc attatgcgtg agtatcccga ccctaaggtt
     9661 tacttctact atgctgaaat gcttaaaatg ggcgaaaagt acaacgaggc cattgagcag
     9721 taccaaaagt acaaggtggc tgttccgctt gaccccttgg ctgatgccgg tataaaatca
     9781 tgtgagctat ccattaagtg gaaggaaaca ccctcggggt atgaaattac caacatgcgt
     9841 agccttaaca cccgtaccag tgagtttagc cctgcttatg cttcgtccga ctatgccacg
     9901 ctttttttca ccagctcacg caatgaggca acaggcggca aggttagctc ggtaacggga
     9961 gagaacttta ccgatatttt tattagcgaa cgtgataata agaatgtttg gagtaagcca
    10021 cgccctattg atgaaaccat aaatacttcc tatgatgagg gaacctgcgc cctaacctcc
    10081 gattacaaca cccttttctt tacccgttgc aaggtgagca agcgcgataa gctgggatgc
    10141 cagatattgg tttccaaacg agccgagcag ggctggctta cacccgatga gctgaaactt
    10201 gccgccgaca gcatgattgt tgcccatcct tcaatttcgg ccgatggcac cgagctgtac
    10261 ttcgttagca acatctccgg aggcattggg gggctagata tttggaagat tacccgaagc
    10321 agctcaacct ccgacgattg gggtgaaccc gttaaccttg gaggcgaggt aaattctgcg
    10381 ggcaacgaga tgttccccta cattcacccc gatggaacgc tctacttttc gtccgacggg
    10441 caccccggaa tgggcggtct tgacatcttc aggtcgtggg tgaacgatga gggtaggagg
    10501 gaggtggaaa acatgcgcta ccccattaac tcctccgccg acgatttcgg aattgtgttt
    10561 gaaaaggatc gcgaggctgg ttttttctcc tcacgccgtc agggtggcag aggcgccgac
    10621 gatatctaca tgttttacct tcccccaatc aacttcaacc tgattgggca tgtaaccgat
    10681 gagaaaagca aaaagcccgt tgaacaggct accgttaagc tggttggaaa cgatggttcc
    10741 gtggttacca tgcaaaccct ggccgatggc tctttcaggt tcaacctcac tcctaacgcc
    10801 gactacgtgg tgattgcctc caagaagggc tacctcaata acaaggcaaa ggaatcgacc
    10861 agagggctta cccagagcaa ggactttgag gtttccattg ccctaacctc caccgaaaaa
    10921 ccgatcgaga tacctaatat tttctacgat tacgataggt gggaacttcg gcccgaatcg
    10981 cgtgaggcgc ttgaccgact cgtggaaatc ctgaacgata accccaacat catcatagag
    11041 ctggcctcgc acaccgatag ccggggaacc aaagagtaca actatgagct ttcccagaaa
    11101 cgcgcccagt cggtggtgaa ctacctgatt gagaaaggta tagagccagt taggctgaag
    11161 gccaagggat atgcccaaac ccaacctaaa gttgttgacg atgccctggc ctccaggtac
    11221 cccttccttc aggttggaac agttttagac cagacgttta tcgatacgct cacctcggac
    11281 gagcaaaagg aaatagttta ccagattaac cgtcgaaccg agttcagggt gcttaaggag
    11341 gattactcgg caaaacctaa gtagctacaa caatccctgt aagcatagaa attgttggtc
    11401 gaaaacttat aaatggagtt caattgatga tttattaagg atgaaaggta accgtttaac
    11461 cgcaataaag ttgaaagtag ctacttacca cctgcttgca gcttttttgc tgccggcttt
    11521 tgcagctggg caaattgcag agtcaagggt tactttcgac atttcggctg ctgaggcttt
    11581 tattgggact tttgaagcca tgcagcaggg aaaatcagaa aaacaggttg acagccttat
    11641 tgatggaatg cttgccctac atgcctacgc tgttagcgcc gaacgctttg gtgcgaagaa
    11701 tcaaatttcg ccggttacgc tcggagagta taagcgtttc ttgaaaagtt tcatctccga
    11761 ttcggttgat gtccagggaa ataccagatt gggaatgcta aaaagccggt atgttgatac
    11821 ctatagtaac ctcccggatt acaaaaagct tgtgaaccag ctcaaagctc agctgaacga
    11881 gtacagtagc ggagctgtgc agcttgctac cgaatggctt cccaaaaatg tggggttgaa
    11941 tgcggaggtg cttgtaatgt tcgacattgg tggagctgcc tgggcataca aagcctccga
    12001 cgggaaggat tatatcatgt atagcgttct gtttctgatg gacgacagca ggaatttcga
    12061 cagcgatatg ttctttaaaa atctggccca cgagctgcac cacgttggaa tcccgctcaa
    12121 cagttttgct aatgaggtgg gctacgacag ccttccccca aatgacaggc ttaagctcta
    12181 cattgatttt atgtatgaca tcgttaagga gggtatggcc caaaaattct gcagcaatgc
    12241 gcctggaaag tttacaccaa aaccataccc taacgagatt tatgccgcta ttccaaaagg
    12301 ggttgaaaac tggaactact ttatctccga ttttcaggcc attaacgact cggttaagtc
    12361 atttctaaac tttatagcct acagcgattc ggtggatgcc ggacaattct acaaccggtt
    12421 tgtccgctac tggacatgga aggcaggcga ggtggagaaa aaagaatttg cgctggatag
    12481 acgctactat tacggcgccg aggttcttgg gcttgtctat accaccctgg ggaaggaggc
    12541 gctgttcgaa atcatgtatg actatcggaa attaccaagc tacttcaacc tagcgctgaa
    12601 aaaggcgaaa cccgagggtt acgagagctt cctatttgac gataggcttg ctgacagcgt
    12661 tttgcacctg aagtgaactg tttgcatgga gaattacatc cggtgcacct agaatgtatt
    12721 ctggctaaat gatagcccac atattcccct tattccacct ctactagctg ggttgagtcg
    12781 gccaaatcgg gctctactgc gattagctct aaagcccttc catgcagcgg cagctgcagc
    12841 gctgtggagt caatggcctg aaaatcattt tcggtgatgt aacccctcat gagcattttc
    12901 gatgcaacat cggtaaaatt ctggtacagg aacggtttca gcctatcctg atcgtcgaac
    12961 aggtacttga attttgttgg tcggggaaca atataggcca ggaataacga ctcctgaagt
    13021 gtaagctcgt tgggcttttt attgaagtag tagctggctg cctcggcaat tccgtaaaca
    13081 tcaggacccc actcaatgat gttcaggtaa atttccaaca tgcggtcctt gctcaccgcc
    13141 gcagtatttt ctattatcca caccattagc atttcctctg ccttacgggc aatgtttttt
    13201 tcctggttca ggtataggtt tttgataagc tgctgggtaa tggtgctccc accacgtgca
    13261 aatctccttt cacgtatatt acaggccaac gcatagcgta tgccatcgag gtcaaatccg
    13321 ccgtgggtat aaaagttgcc atcttcactg gtgattatag cccatttcaa gaatggcgaa
    13381 atgtggttta gcggagtaaa ttcaggatta tcggagctaa cccgaatgtt gcgaatccat
    13441 ctgccatcct tgtaaaccct atgggtaaag gtatcgttaa gggcgtaaaa gttggtttgt
    13501 ccaaatgagc tgattccaaa cccgctggag cttagcagcg gttcaataac caggctatct
    13561 ggactttcag ggtttaccct ggctttaaga gtgtagctga atgttcccct aagtttcatc
    13621 ccggcaatat gggtgaataa acctgaggga agcgactcca ccagatcctg ccagcgggta
    13681 gtagcggttt taaacttcaa cgtaagggtt ggagatttgt ccagggtgta acacgacttg
    13741 aagctggttg aaagtcggtt taagcttaac gttgaggttg aatcgaggca gatttcgctg
    13801 ggcttgagct ttatgtccag cttaagcgaa gcgctgtcta tattcatcgg gtatggggct
    13861 attctgcttc cctccatcga gatgtttttg gccatggtct caatgtttaa tgccataagg
    13921 ttaccatgca gctcgcctgc acgaaagtca agtttcgcgc tatccaggta cactcctacg
    13981 cctgtgagcg cttcgataaa tgggatttcc acctgaaacg agcgaccata aacccatacc
    14041 ctgaagctgt tacggctctt gctcgattcg ccggccagct caaagctacc ccttctatcg
    14101 ccatgctggg tagctaccga tgcggagaaa cggctgtttt tgtaattcca actggttact
    14161 gtagccgttt cgtagtgcaa cgagtcgatg tacgaaatgg tcaaattatc aatgttgatt
    14221 ttagctgttg ataggccaaa tgtgcccttg ataactttat aaatgggtag caggttaaat
    14281 ttctcctgtt tgttggcttc cgctgtaatg gcttctttat tttgaatgga atttcgttca
    14341 agcaacgtat ttaggttaaa ccatgaatcc tttagctcta tggccagcgg gttaaccttc
    14401 aatacaagca ggtgaagtaa acccagcttg atgcgagcag ctccaacctg ggcaagggta
    14461 tccgcatggc tgttgagcac aaccaggttg ctaatatcca gcgttctgat tcccctgaat
    14521 tttagcctgt ctgctgccac ttgtaggttg tatgccgttg ttagcctctc agtcttttgc
    14581 tcaaaaacgt agcttaacat cctgtttctg aacagtccgt ataaaaccag tatcagccct
    14641 atggttgctg taatgatggc tgtaaccctt tgtctgcgct gcataaggtt taattttatg
    14701 atgtagctct ccggagctag ctgcttggtt ttgggtagct gtccgcagca ttatgccgct
    14761 ggtcgggagg ctagaatgaa tactttatgt taagggttgc acggtggttg taaacgggat
    14821 cgagcgatga ggtaaccctg tgcctgttgt caaatgtact accataagta gcggctgtca
    14881 gcatgtactc cagggcaaag gtaatacttt ccgcctggta tgatagacgg ggtgaaatcc
    14941 tgtagatgtt gttcaggtcg tcgttacggg agtaacccgc aaggcttgtg tagtttttgt
    15001 cggcgccgta aagtttagag tacccggcaa acaggccaac tcccattttc cccgcctggt
    15061 gctgaacatc aacccatgtg ctaagggtgt taaggctggt gtagtcgtaa tcatcgttgg
    15121 gagctggacc ggaaccggaa gccattccgt aaccgccaat catattcagg tgggttgggt
    15181 tctgcccgta aactgcttct gccttaacaa cagttttacc aagttttttc aataaaaatg
    15241 cgctaaaaag gtactggccg gatgttttac acgtggcata gcctgtttgg gttacaagcc
    15301 tgggggtgag ccacttatat ccggccgaca acccggccaa aaattcttca gcactacctg
    15361 cagcggcttg cagcattagc tcgggtttgc cggagttacg ctgggcatcc tcggggccgg
    15421 atgacttgtg gtagctttgc atgagggcgg taagcgagat tctgaccata gggtaaggga
    15481 agtagctgaa gcgaatctgg ggcgaacggt tgagcgagtg gaacggcgct cccgagccaa
    15541 acgaaattac tgatggtgca acatcggtaa caaaaaccgg gtgccagtac tggccaacca
    15601 gcagctcggt ggtagcccac tttagcttga acattgcatg cctgatgcgc aacaggctca
    15661 cgtagtcgtt tttggtagcg tagaaatcag cttcgaccag cgcggacgat tttgccccaa
    15721 gtatatctgg tcccctaacg gaaagcccca gccgggtgga tatgctcagc atctgaagct
    15781 gaactgcctt gttgatgtcc ttcccggtgc tatcgagccg tggtcgcaag gggtaaaaga
    15841 ttagctcacc gtcgcgggca tcgagcgact tgtaggtgtc cagaatggca tcgtag
//
LOCUS       DAOSHM010000054        15620 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_3087_length_15620_cov_19.595952, whole genome shotgun
            sequence.
ACCESSION   DAOSHM010000054 DAOSHM010000000
VERSION     DAOSHM010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 15620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15620
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3087_length_15620_cov_19.595952"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(536..1618)
                     /gene="wecB"
                     /locus_tag="PK839_08325"
     CDS             complement(536..1618)
                     /gene="wecB"
                     /locus_tag="PK839_08325"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HOG72778.1"
                     /translation="MNSKLILTVLGARPQFIKAAAVSRQLMAFGLEEVIVHTGQHFDE
                     NMSEIFFKEMCIPAPKYNLEVHGLNHGAMTGRMMELLENIVINEKPAAVMVYGDTNST
                     LAGALVAKKLNTRVVHVEAGLRSFNMTMPEEINRILTDRISDLLFTPTEAADSNLMAE
                     GFSHFNAKIIRSGDVMQDAAYFYFNRAKTESHVLERLNLPEHFVLATFHRQENTDNLN
                     NLQGIVDGLNRINSETQVVVPLHPRTAKILERVSISPNFRIVEPLGYFDMLMLLNQSS
                     LVVTDSGGLQKEAFFFGKHCVTLREQTEWVELVEKGYNMLSGTSSDSIYNSFRQMIAR
                     KSNFKVNLYGNGHAAEKIAAELKDWL"
     gene            complement(1611..2927)
                     /locus_tag="PK839_08330"
     CDS             complement(1611..2927)
                     /locus_tag="PK839_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOG72779.1"
                     /translation="MEKNLRRRILVITYYWPPSGGVGVQRWLKFVKYLRLYGWEPVVY
                     TPENPESFYDDESLLNDIPEGVEVIKTRIWEPYKIYKRITGTKNEKIAVGFTSSSNKK
                     KGLLHRVAVWLRGNLLIPDPRVFWVNPSVKFLKKYLRENPVDVVVTTGPPHSIHLIGL
                     KLKQKMKVKWLADFRDPWTNIDYIKDLRLSAPAMRIHRRLESKVVKEANRVVVVSEQM
                     KREFQQLAAKRIDVITNGFDEDDTPIKLIPLDEKFSITHVGTLTPSRNTPMLWQSLAE
                     LCVEIVGFRNDLIIRLVGQVDISIIESIRSVGLDGNLESLGQVSHAEAMMFQKKSQVL
                     LLIVNNSPNATGIITGKVFEYFASGRMVLAVGPTYGDLASLLSETKAGFFVDYNDKAK
                     TKRVITHLYNLYKENNLRPETKDILRYSRKELTRTLSNLLNEMTNE"
     gene            3388..4392
                     /gene="amrS"
                     /locus_tag="PK839_08335"
     CDS             3388..4392
                     /gene="amrS"
                     /locus_tag="PK839_08335"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HOG72780.1"
                     /translation="MEAKYYSKKGNGIRCNLCPHSCSIQDGRFGICKARQNQGGVLNA
                     LGYGVISSSGLDPMEKKPLYHFHPGSRIFSIGSYGCNLHCDFCQNFEISQSFPVSMPP
                     LRSYQPSEVVEKARVLPENIGIAYTYNEPTISFEFMLETARLAKSKGLLNVAVSNGYI
                     NPAPLRELLEVLDAFNIDLKSFSDDFYRSQTGGRLKPVLKTLVTIRELGKHLEIAFLV
                     IPGLNDSLAETKAMVSWIASNLGSNTVLHINRYYPAYKLKIPPTPEERLVELYEIARE
                     KLAYVYLGNLHGNEVGCNTICPSCSSTVIRRNGYHTVISALTREGRCSSCGYGPIVVM
                     "
     gene            complement(4465..5052)
                     /locus_tag="PK839_08340"
     CDS             complement(4465..5052)
                     /locus_tag="PK839_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOG72781.1"
                     /translation="MLKDLVLQNRSYRRFYQEKNVDRESLVELVELTRFCPSGRNAQP
                     LKYFISNSEPTNSHIFSNLAWAAYLKEWNGPEEGERPSAYILILEDQTIATSSQRDQG
                     IMAQTIMLGAAEKGLGGCIIASVKGHELAKILGIPDHLKIVLALALGYPKEHVVIEPM
                     PQNGNIEYWRDDKGVHHVPKRDLSELIWNKTVGKG"
     gene            complement(5138..5713)
                     /locus_tag="PK839_08345"
     CDS             complement(5138..5713)
                     /locus_tag="PK839_08345"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HOG72782.1"
                     /translation="MKHELPQLPYSADALSPVISAKTIEFHYGKHHQAYVNNLNNLIV
                     GTPFENATLETIIKEAEGGIFNNGAQVWNHTFYFLTLSPNPKSEPSGKLGEQISSDFG
                     SFAEFKDKFEKASAGLFGSGWTWLVLNSKGELEITQESNAGNPLRKGLKPLLACDVWE
                     HAYYLDYQNRRPDYLHSFWNIVDWKVVEGRF"
     gene            complement(5744..8188)
                     /locus_tag="PK839_08350"
     CDS             complement(5744..8188)
                     /locus_tag="PK839_08350"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG72783.1"
                     /translation="MNRLFKYFTFLILAIPTGLPRMAAAMVNKATPDTLQINGNLNTV
                     RSLITSREFSAAAMLTKEALSNSRKINYIKGVAWSEYYLGMICFENSTLDSAIYWLKR
                     SENDFAAVRDYSARVKACNLLGIYYRQSDSNDSSLDCHTRALKIAMENNDSAGIAESH
                     YGIGMTLNQLGYYPETMEHFHAAISIREKLNDSSGVAAILNSMGILFRNQGDYANALD
                     FYVRALNIHEKLGNIQGLAYQHNNMGLIYRDIGDYGKALDHFNKSWALKVKINDSRGI
                     SNSIMNIGSVYLLQNKLDSALIFFDQAASIKEQIGDKSGIANINLFRGECYQKLGLYK
                     KALDFLESSRSDYHALKEPRGETEAIIQLASTYFNSGMHDKAFQLIDEAKNMVVTLKM
                     LDLQQKVFLNLYNFYIELGDYRKGLEYYQLYIAARDSVMGTRNLRKMLNVELQDEYSR
                     LIKEHFERKDQEILSKEQAQQSKTVLLYILSGAFIILLLFLVMFIRTLKNKQKINNML
                     IAQQAEVEHQKQELMNQRDEIDTQKEMVVYQRDKIITMLTDLAESIDYAKKIQQAILP
                     KDSLLQQLFTDYFIIYQPKEAVGGDFYWAGRFNGTICFAVADCTGHGVPGGFMSMLGV
                     SMLNDMATADSVQSPSLMLQSLRHNIVAALGQTNFDEDNHDGMDIAFCTFDIKSGILT
                     YAGANMPIIINSKGEIPPSERIQPYQNGLYELKPDRMPVALYDKMDSFSEIRVKLSPG
                     DAVYLFTDGYVDQFGGPKSKKFGHDAFRTLINSVKDLSFSEQKQIIWSTIERWKGETE
                     NQTDDILVMGLKLPDK"
     gene            complement(8335..8943)
                     /locus_tag="PK839_08355"
     CDS             complement(8335..8943)
                     /locus_tag="PK839_08355"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HOG72784.1"
                     /translation="MSFQLPTLKYDYKALEPYIDAMTMEIHHSKHHATYTNNFNAALE
                     GSSLASLPVEEIFKDVSKHPVAIRNNGGGFYNHSLFWDIMSPNGGGEPTGELLKAIQK
                     SFGSFSEFKEKFSSTATGRFGSGWAWLVLSKGELKIYSSPNQDNPLMDISEVKGYPIL
                     NLDVWEHAYYLKYQNRRPEYIQNWWNVVNWSAVEKRFLEGLK"
     gene            9221..11092
                     /locus_tag="PK839_08360"
     CDS             9221..11092
                     /locus_tag="PK839_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011107811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HOG72785.1"
                     /translation="MASIYLQAENLTKSFGDRILFGNLSFSLFQLDRTAIVAKNGSGK
                     STLLSILAGKQHPDSGSVVTRNGIRLGYLEQNPVLRDDYTILESVYSADNDISRAVQD
                     YERAMATSDKDHLQQAIETMDRLEVWGRETKAKQILTRLRIENLNQPIANLSGGQRKR
                     VALASVLIEEPDILILDEPTNHLDLDMVEWLEDYLIHGQQTLLMVTHDRYFLDRVCGC
                     ILEIDNQSLFRYKGNYSYFVEKRQDRINALASEVEKANNTLRYESEWIHRMPKARGTK
                     SKYRIDAFYKLREKTLLQNLESPMEINISASRLGKKVMEVKNISKAFGSKVILRDFSY
                     TFNRFEKLGIVGRNGTGKTTFLNIITGNTLPDSGLVDIGSTVAIGYYKQQDVSFPNNA
                     RVIDIARDIAEVVTIADGKTITVSQFLNMFLFPPEKQHDFVQKLSGGEKRRLYLLTVL
                     MRSPNFLILDEPTNDLDIQTLCILEEYLKSFPGVVVVVSHDRFFMDKVVDGLLIFEGA
                     GEVSGFPGSYTELREWTLEQQNDCPQGKPSQKSVKTGSSRVRIKQRLSFAQKKELEHL
                     TSEIELLEREKLELEQQLSSGMLDASKLQEKSEQYGHLLERLGNIEMRWLELSELEG"
     gene            11390..>12045
                     /locus_tag="PK839_08365"
     CDS             11390..>12045
                     /locus_tag="PK839_08365"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOG72786.1"
                     /translation="MVNFRNRIAWVTGASSGIGKEIALALAREQAHLILSAKNPEELE
                     VVASECRKYTPFCHEVPFDLSDPEEVERIAKSVVNTYGHIFLLINNGGISQRSLAVET
                     PVEIDRKIMEVDYFSHVIITKTVIPGMIEAGVGYIAATSSISGKFGFMYRSAYAAAKH
                     AIQGFFETLRVELKPHNISVTIAYPGRIRTSISLHALDKEGNEYGKMDPGQSNGLAPQ
                     "
     assembly_gap    12046..12145
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <12146..12342
                     /locus_tag="PK839_08370"
     CDS             <12146..12342
                     /locus_tag="PK839_08370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="short-chain dehydrogenase"
                     /protein_id="HOG72787.1"
                     /translation="NEYGKMDPGQSNGLAPQKCAKRYIRAVKRRKPEVLIGKSELLMV
                     HIKRLLPSLFYKMVSRVKPV"
     gene            12355..13431
                     /locus_tag="PK839_08375"
     CDS             12355..13431
                     /locus_tag="PK839_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOG72788.1"
                     /translation="MEKIIYGIQQVGLGVSNLEEVWKWYIDSLGFDVKIFDDDTVAEL
                     MLPYTGGEPQQRHAILALNLQGGGGLEIWQYKERTPLPPKFPVKLGDLGIFAAKIKSP
                     DIEAAFNFLKSKGATLGDLVNSPEGKPHFYISDPFGNTLEVYEDKSTFLNEGRSTSGI
                     GGAIVGVSQMERSVKFYSRVLGYDKVVYNKSGRFDDLAGLPAGNENFHRVLLTHSDTR
                     KGSFSELFGQSYIELIQPLDRVPLKILQDRFWGDLGFIHLCFDVKNMDQLERECNELG
                     FPFTVNSNIKHSDKGSFDMGEAAGHFTYIEDPDETLIEFVETHFLPIYKPLGIKVNFQ
                     NRRPYKSVPRWMIKLLRFKRVKKI"
     gene            13925..15136
                     /gene="rocD"
                     /locus_tag="PK839_08380"
     CDS             13925..15136
                     /gene="rocD"
                     /locus_tag="PK839_08380"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018361221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine-oxo-acid transaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HOG72789.1"
                     /translation="MTPKDYMDRESKYGAHNYHPLPVVLEKGKGVYLWDVEGKKYYDF
                     LSAYSAVNQGHCHPKIVKALTDQAQLLTLTSRAFYNNVLGEFEEYVTKYFGYDKVLPM
                     NTGAEGDETALKLCRRWAYDVKGIKDGEAKIIVCENNFHGRTITVISMSTDPDAYRGY
                     GPYTPGFIKIPFNNIPALEDALKDKNVAGFLVEPIQGEAGVFVPDDGYLKKAYELCKK
                     HNVLFIADEVQTGIARTGKMLACDHEGVRPDILILGKAISGGVVPVSCVLADDEIMLT
                     IKPGEHGSTYGGNPLACKVSIAALEVIKNEKLVENAAKMGEIFRREMRNIQSDMIELV
                     RGKGLLNAVVIRNKPGKTAWDVCLAMADKGVLAKPTHGNIIRFAPPLVITEKELLDAI
                     SRIKEAFKLFE"
BASE COUNT         4203 a         3296 c         3539 g         4482 t
ORIGIN      
        1 gacggaactt gtccgcctcc tggcggattc acctaacatt aaaaagggtt ttaaagagcg
       61 gcatcggccg gcgagccgaa aaacaagcgc aggcggcgta gaaaaaatct gctgttcgaa
      121 gcgctgagcg gagcgaggcg caagttcaga ttttttagcc gccaagcgta gctttttcgt
      181 gcgaggcggg cgcagccttg actttttttg catacttttt tgtgtcaaga caaaaaagta
      241 tgtggggttt ggggcaaagc cccaagattt catgataaaa acttatccag cttttaacaa
      301 actcacaaat tcattctgca cctttatggc ttaacacttc tctccggaac gccaaaagga
      361 actttgcccc atgctagcta ttgcgtcccg ttggggctga agaaaaagta aggtaatctt
      421 gcccggcttt ttacctttaa cgcttcacaa tgcacatctg ctcttctgga ttttattttc
      481 ttcctctttg cttcaaaatg ggttgtaaga gtttttattg aatctgggtt ttagcctaaa
      541 gccaatcctt tagctctgct gcaattttct ccgctgcatg cccattcccg taaaggttaa
      601 ccttaaagtt tgattttcgg gcaatcattt ggcggaagga gttgtatatg ctgtcggagc
      661 tggttcctga aagcatgttg tagcccttct caaccagctc aacccattcg gtttgctcac
      721 gtagggttac gcaatgcttt ccaaagaaaa atgcctcttt ctgtaaaccg ccgctatcgg
      781 taaccaccag cgaggattgg tttagcagca tgagcatatc aaaatagcct aagggttcaa
      841 ctatcctaaa gttgggtgaa atacttaccc tttcaagaat ttttgctgtt cggggatgta
      901 ggggaacaac tacctgagtt tcagaattta tacggttcag cccatcaact attccctgaa
      961 gattattaag attatcggtg ttttcctgtc tgtgaaaggt agccagcaca aagtgctcgg
     1021 gtagatttaa gcgttcgagt acatgtgact cggttttagc cctgttaaag taaaagtagg
     1081 ctgcatcctg cataacgtcg ccactcctga taattttggc gttgaaatgg ctgaatcctt
     1141 cggccatcag gttgctgtcc gccgcttctg ttggggtaaa aagtaaatcc gatatccggt
     1201 cagtaagaat gcggttgatc tcctcgggca tggtcatgtt gaacgagcgg agtcctgctt
     1261 caacgtgcac cacgcgggtg ttcagctttt tggctaccag tgctccggca agtgttgaat
     1321 tggtgtcgcc ataaaccatt actgctgccg gtttttcatt aataacgatg ttttcgagca
     1381 gctccatcat tctaccagtc atggcgccat ggttcaaacc atgaacctca aggttgtact
     1441 ttggagcggg aatgcacatt tccttaaaaa atatctcgga catgttttca tcgaaatgct
     1501 ggccggtgtg cacaatcact tcttccaatc caaaagccat aagttggcgg ctaactgcgg
     1561 ctgcctttat gaactggggt ctagccccta aaacagtaag tatcaacttg ctattcatta
     1621 gtcatttcat ttaataggtt tgataaggtg cgggtgagct ccttacggga gtaacgcaaa
     1681 atatccttgg tctctggccg caggttgttc tctttataga gattgtagag atgtgtaatg
     1741 acccttttag tttttgcttt atcgttatag tccacaaaga atcctgcttt tgtctcggaa
     1801 agcagggagg ccaaatcgcc ataggtgggt ccaactgcca gcaccattcg ccccgaggcg
     1861 aagtactcga atacctttcc ggttattatt cccgtggcat ttggggaatt gttgacaatc
     1921 agaagtagca cctgcgattt tttctgaaac atcattgcct cagcatgtga aacctgaccc
     1981 aaactttcca gattaccatc aaggcccacg ctgcgaatgg attcaataat agaaatatct
     2041 acctgtccaa ctaatctgat tatcaaatca ttgcgaaaac caacaatttc aacacataat
     2101 tctgccaggg attgccaaag cataggggtg tttcttgaag gagtaagggt tcctacatgg
     2161 gtaattgaaa atttttcgtc aagtggtatt aattttattg gagtatcgtc ttcatcaaag
     2221 ccattggtta taacatcaat ccttttggca gcaagctgtt ggaattccct tttcatttgt
     2281 tccgacacca caaccacccg atttgcttcc tttaccacct tgctttccaa cctgcgatgg
     2341 attcgcattg caggagcgct tagtcttaaa tccttaatgt agtcaatatt tgtccatgga
     2401 tccctaaaat cggctagcca ttttactttc atcttttgtt tcagcttcag cccgattaga
     2461 tggatggagt gcggtgggcc ggttgtaacc actacatcca ccggattttc ccttaggtat
     2521 ttttttaaga acttaaccga tgggttaacc caaaagactc ttggatcagg aataagcagg
     2581 ttgcccctta gccagacagc caccctatgc agtaatccct tttttttatt tgaagaagag
     2641 gtaaaaccca cggcaatttt ttcgttcttt gtgccggtga ttcttttgta tattttgtag
     2701 ggctcccata ttctggtttt tataacctca accccttcag gaatgtcatt cagcagcgat
     2761 tcatcgtcgt aaaacgattc gggattttcg ggtgtgtaaa ccaccggttc ccatccgtac
     2821 agcctgaggt actttacaaa ctttagccaa cgttgaacgc ctacaccacc tgatgggggc
     2881 cagtagtagg tgataaccag aatacgcctg cgtaagtttt tctccattta ttaaataaaa
     2941 gttcaaaggt aaaaatttgg ccgcaacccg gaaaggaata gtagtggaag ttccctaatt
     3001 tcagattaat ctctttaatg tttttacttt acagcggctt catgcagtcc tttactacta
     3061 tgacctacat gtaaatacgg gagttattta atcgtcaaca ttatcatgca aatcctaaat
     3121 ttaatttgtg ttggtcaagc tatgcaggaa gcaggtaaac ttcaaaattt ctgatatcca
     3181 acaggtgtaa aacatacagg tactattttt aatgattttt gtgggatgat ttctgcgttg
     3241 aagaagtgaa aaagcattca acactcgatt ttttaaattt tgttttaacc cataagtatt
     3301 gtatcgttgg aattaagttg ggatgacttg caattaaagc aaaagtttat cgaactttga
     3361 tgataattct aaatgtgctt tttgatcatg gaggcaaagt actattcaaa aaagggtaac
     3421 ggcataaggt gtaacctttg ccctcatagt tgtagcatac aggatggaag gtttggtata
     3481 tgcaaggcac gtcaaaacca gggtggggtt ctcaacgctc taggatacgg ggtaatttcg
     3541 tcatcggggc tagacccaat ggaaaaaaag ccgctttacc actttcatcc gggtagccgg
     3601 atattttcta tcggcagcta cgggtgcaac ctgcactgcg atttttgcca gaacttcgag
     3661 atttcgcaat ctttccctgt ttcgatgccc ccgttgagga gttatcagcc ttcagaagtt
     3721 gttgagaagg ccagggttct tccggaaaac attgggattg catataccta caacgaacca
     3781 acaatatcat ttgagtttat gcttgaaacg gcaaggttgg ctaaaagtaa aggtttgctg
     3841 aatgttgcgg tttcaaatgg gtatatcaac cctgcaccat tgagggagct tcttgaagtt
     3901 ctggatgctt tcaatatcga tttaaaatca ttttccgatg atttttaccg cagtcaaaca
     3961 ggaggaaggc tcaagcccgt gctgaaaact ctggtgacca taagggaatt gggtaaacat
     4021 ttggagattg cctttctggt cattccggga ctaaacgatt cgcttgccga aaccaaggca
     4081 atggtttcgt ggattgcttc gaatctggga agcaacacgg ttttgcatat caaccgctac
     4141 taccctgcct acaagctgaa aattccgcct actcccgaag aaaggttggt tgagctatac
     4201 gaaatagctc gagagaagct agcctatgtg tacttgggca acctgcatgg aaacgaggtg
     4261 ggctgcaaca ccatttgtcc gtcgtgttcc tccacggtta tccgtaggaa tggctatcac
     4321 accgttatct ccgccctaac ccgtgagggt aggtgcagca gttgtggcta cggaccaatt
     4381 gttgtaatgt aaggctgcta agctattcct gttgaagaat agcgttggat tacctggctt
     4441 tggcgattag aagaatgtaa ttgtttaacc tttaccgact gtcttgttcc aaatcagctc
     4501 gctaaggtca cgttttggaa catgatgtac acccttatca tcccgccagt actctatatt
     4561 tccattttga ggcattggct caatcacaac atgttctttt gggtagccta acgcaagtgc
     4621 cagcacaatt ttgaggtggt cgggaatacc cagtatttta gccaattcat gacctttaac
     4681 cgatgcaatg atacaacctc caagcccctt ttctgcagca ccaagcatga tggtttgagc
     4741 cattattccc tgatctctct ggctggacgt ggcaatggtt tgatcctcaa gtatcaggat
     4801 gtaggctgat ggtctttctc cctcttcggg gccattccat tcctttagat atgcagccca
     4861 tgccaggttg gaaaatatat ggctgtttgt tggttcggaa tttgaaatga agtactttag
     4921 aggttgtgcg ttacgacccg atgggcaaaa cctggtcagc tcaaccagct caaccagcga
     4981 ttctctgtca acattttttt cctggtagaa cctacgatag ctgcggttct gcaacaccaa
     5041 atcttttaac atggttttaa ttttttacaa attattggat aaaagaaata cccggtaagc
     5101 ttaattccta ccaggtaagt ttaaaaaaat cttttagcta aaaccttccc tctacaactt
     5161 tccaatcaac tatattccag aagctatgaa ggtaatcggg ccggcgattc tggtaatcga
     5221 ggtagtaggc gtgttcccat acatcacacg ccaaaagagg ctttaggcct ttgcgaaggg
     5281 gatttcccgc gtttgactcc tgggtaatct ccagctcgcc ctttgagttt agaaccaacc
     5341 aggtccatcc ggaaccaaac aggccggctg aagccttttc aaatttatct ttaaattctg
     5401 caaatgagcc gaagtctgaa gaaatctgct ctccgagttt tcctgacggt tcgctttttg
     5461 gattgggcga aagagtgagg aagtagaagg tgtgattcca aacttgtgca ccgttgttga
     5521 aaattccacc ctctgcctcc ttgatgatag tctcaagtgt ggcgttctca aacggtgtcc
     5581 caacaataag gttgttgaga ttgtttacgt aagcctggtg gtgctttccg taatggaact
     5641 caatcgtttt ggcagaaatt accgggctca aggcatcggc tgagtacggt agctgcggca
     5701 gttcatgttt catcttggtt aattttaaag gttacactgt tttttatttg tcggggagct
     5761 ttagtcccat aaccaggatg tcatcggttt ggttctcggt ttctcccttc cagcgctcga
     5821 tggtactcca aatgatttgc ttctgctcgg aaaagcttaa atctttcact gagttaataa
     5881 gtgtacggaa ggcgtcatgt ccaaacttct tactttttgg cccaccaaac tggtcaacat
     5941 atccatcggt aaaaaggtaa acggcatctc ctggcgatag cttgacgcga atttcgctaa
     6001 aggagtccat cttatcgtaa agtgcaactg gcattcggtc tggttttagt tcatagagcc
     6061 cattttgata gggctgaata cgttcggagg gtggtatttc gcctttagag ttaataatga
     6121 taggcatgtt ggctcccgcg taagttaaaa tacccgactt gatgtcgaag gtgcagaagg
     6181 caatgtccat cccatcatgg ttatcctcat caaagtttgt ttgcccaagg gcagccacaa
     6241 tattgtgcct gaggctttgc agcattaatg atggggattg aacgctgtcg gcagtagcca
     6301 tatcgttaag cattgaaact cccagcatgc tcataaagcc tccagggacg ccatgtccgg
     6361 tacaatcggc tactgcaaag caaatggtgc cgttgaaccg gccggcccag tagaaatcgc
     6421 ccccaacagc ctccttaggc tggtatatta tgaagtaatc ggtaaatagc tgctgcagga
     6481 gggaatcttt tggtagaatg gcttgctgaa ttttttttgc atagtcaatt gattctgcca
     6541 aatcggtgag catggtaata atcttatcac gctggtaaac caccatttct ttctgggtgt
     6601 caatctcgtc gcgctggttc atcagctcct gtttttgatg ctccacctcc gcctgctggg
     6661 ctataagcat gttatttatt ttttgtttat tttttaaggt gcggatgaac attaccagga
     6721 atagcagaag aatgataaat gcccccgaaa gtatatagag taaaactgtt ttgctttgtt
     6781 gtgcctgttc tttagacaaa atctcctgat cttttcgttc aaagtgttct ttaataagtc
     6841 gggagtactc atcttgcagc tcaacattaa gcattttcct taagtttcgt gtacccatca
     6901 cactatcgcg ggcggcaatg tatagctggt agtattccag tccttttcta taatctccga
     6961 gttcgatata aaaattatag aggttcagga aaactttctg ctgcaggtcg agcattttca
     7021 atgttactac catattttta gcttcatcga tcagctgaaa ggccttgtcg tgcatacccg
     7081 agttaaagta tgttgaagcc agctggatga ttgcttcggt ttcaccccgg ggttctttta
     7141 gagcatggta gtcacttctg gatgattcga gaaagtctaa agcttttttg taaagaccaa
     7201 gtttttggta gcattcaccc ctgaataaat taatattggc aattccactc ttatcaccta
     7261 tctgctcctt tatggaggcg gcctgatcaa aaaatattag tgcgctgtcc agcttgttct
     7321 gtaaaaggta aaccgaacca atattcataa ttgagttgga aattccccgg ctatcgttta
     7381 ttttaacttt caaggcccat gatttgttga aatgatcgag tgccttaccg taatcgccaa
     7441 tatccctgta aattagaccc atgttgttgt gctggtaggc taatccctga atatttccca
     7501 acttctcatg aatgttcaat gcccggacat agaaatctaa tgcattggca taatcgccct
     7561 gatttctaaa gaggatgccc atgctattca gaattgccgc tactcctgag ctgtcattta
     7621 gcttttccct tatcgagatg gctgcgtgaa aatgctccat ggtttcgggg taatagccga
     7681 gctggttgag ggtcatacca attccatagt gcgactcggc aataccggcc gagtcgttat
     7741 tttccatggc aatctttaaa gctcgggtgt ggcaatccag ggagctgtcg ttgctatcgg
     7801 attggcgata gtaaattccc agcaggttgc atgcttttac ccgtgcacta tagtcgcgaa
     7861 cggcagcaaa atcattttct gatcgtttta gccagtatat tgcagaatct agtgttgaat
     7921 tctcaaaaca tatcattccc aggtagtact cactccatgc aacaccttta atgtagttga
     7981 tttttcttga attgctgagg gcttctttgg tgagcattgc agctgctgaa aactctcggg
     8041 aggttattag cgacctaacc gtattcagat ttccattaat ttgcagggtg tccggtgtag
     8101 ccttatttac catggctgca gccattcttg gcaagcccgt ggggatagct aaaattaaaa
     8161 aggtaaagta tttgaaaagt ctattcatca gcagtttatt ataggcagaa tagcatattt
     8221 tataagctta aatttacaaa agtataacca aaataaaaac agcagtacaa aaaagaggct
     8281 gggaaattgt cccagcctca aaaccaaata acaaaacaaa atgaccctgg caggttattt
     8341 taagccttct aaaaatcttt tttcaacggc cgaccagttc accacattcc accagttctg
     8401 aatgtattcg gggcgacgat tctggtattt aagatagtag gcatgttccc atacatcgag
     8461 gttgagaatg gggtagcctt ttacctccga tatatccatc agggggttat cctggttggg
     8521 agaggagtat atctttaatt ctccttttga tagcacaagc caagcccagc ctgacccaaa
     8581 ccttccagtt gcagtggatg aaaacttttc cttaaactcg ctgaacgatc caaaactttt
     8641 ctggattgcc ttaagcagct caccggtagg ctctccaccc ccattgggag acatgatgtc
     8701 ccaaaacagg ctgtggttgt aaaatccccc gccgttgtta cgaatggcaa ccggatgctt
     8761 ggaaacatcc ttaaaaattt cctcaacggg aagtgaggcc agcgagctac cctccagggc
     8821 agcattaaag ttgttggtgt aggttgcatg atgctttgag tggtgaatct ccattgtcat
     8881 ggcgtcaata taaggctcaa gagccttata atcatacttt agtgttggta gttgaaatga
     8941 catagcttta tcgtattatg ttatttattc tcattctaaa tagagtaacc aacatgtagc
     9001 aatattgttc agcgttgttg gaaattttct atgtttaaga caagatacat gaaacggatt
     9061 tcgacctgca gctgatttgc tgattcgtca aggggttgtt gctagtaaat acttaaaaaa
     9121 cttgagaggt gtataagcat cagccttcaa cctgccagca gcatgttgtt tgtatttagc
     9181 catttatact taactttgcg ggtcaaattc aactattttt gtggctagca tataccttca
     9241 ggctgaaaac cttacaaaat cttttggtga tagaattctc ttcggtaact tgtcgtttag
     9301 cctattccag ctcgatagaa cggctattgt agcaaagaac ggttccggaa agtcaacgct
     9361 tctgagtatt ctggcaggaa aacagcaccc cgattcgggc agtgttgtta caagaaacgg
     9421 aataaggctg ggctacctcg agcaaaatcc ggtgttaagg gatgattata caattctgga
     9481 aagtgtttat agtgccgata atgatatttc tagggcagtg caggattacg agagggcgat
     9541 ggcaaccagc gataaggatc acctgcaaca ggctatcgaa acaatggaca ggttggaagt
     9601 atggggaagg gaaaccaagg caaagcaaat tttaacccgg ctacgaatcg aaaaccttaa
     9661 tcagccaatt gccaatcttt ctggtgggca acgcaagcgg gtagctcttg catcagtcct
     9721 cattgaagaa cctgatattc ttatactcga cgaacctacc aaccatcttg atttggatat
     9781 ggttgagtgg ctggaggact atttaattca cgggcagcaa acccttttga tggttacgca
     9841 cgacaggtac tttcttgaca gggtttgcgg ttgtattctc gagattgaca accagtcctt
     9901 gttccggtac aaaggaaact actcctactt tgtggaaaaa cggcaggata ggattaacgc
     9961 ccttgcttca gaggttgaaa aagccaataa cacgctaaga tacgagagtg aatggataca
    10021 ccgaatgcca aaggccaggg gcacaaagtc caagtatcgt attgatgctt tttacaagtt
    10081 aagggaaaaa accttgctgc aaaacttgga atcaccgatg gaaattaaca tcagtgcctc
    10141 ccgattaggg aaaaaggtta tggaagttaa aaacatatct aaggcttttg gaagcaaggt
    10201 tattctcagg gatttcagct atacatttaa caggtttgag aagctgggaa ttgttggtag
    10261 aaatggaacc ggtaaaacaa cttttctcaa cattatcaca ggtaatactc ttcccgatag
    10321 cgggttggtt gacattggca gcacggtagc tatcggctac tataagcagc aggatgttag
    10381 ctttcccaac aatgccaggg taatagacat tgccagagat attgccgagg tggttaccat
    10441 tgccgatggc aaaacaatta ccgtttcgca atttctcaac atgttccttt tccctcccga
    10501 aaagcagcac gattttgtcc aaaaactaag cggaggggag aaacgcaggt tatatctttt
    10561 aaccgttctt atgcgtagcc ctaactttct tatcctcgat gaacccacca acgacctcga
    10621 cattcaaacc ctttgcatcc ttgaagagta tcttaaaagc tttcccggag ttgtagttgt
    10681 ggtttctcac gaccgttttt ttatggataa ggttgttgat ggcctgctga tatttgaggg
    10741 cgccggcgag gtaagcggtt tccccggatc gtacaccgaa ttgagggaat ggactcttga
    10801 gcagcagaat gattgcccgc agggcaaacc ttctcaaaaa tcggtaaaga ccgggagtag
    10861 cagggtgagg attaagcagc ggctttcatt tgcccaaaaa aaggagctag aacacctaac
    10921 ctccgaaatc gagctgcttg aaagggaaaa gctagagctg gagcagcagt tgagtagcgg
    10981 aatgctggat gccagcaaac tgcaggagaa atcggaacag tatggacatc tactcgaaag
    11041 gctgggaaat attgagatgc gatggttaga gttaagcgag cttgaaggtt aggtagccac
    11101 attaaagtac caacctcttg gttgaaatta ctggataata gttgttttta gactggaaaa
    11161 tcaggtggga gaaagcaagc aggtaaacgt ttcatgttta tttttgtgtg agcattggga
    11221 aatagcagga atgaagttaa tgtaaaaatt tctcaaaatg gaacatttga gtacggctgt
    11281 gctttggatt gcctcccctg cttcctttta ttcaaatgta aaaagagcta aatttgccac
    11341 tcaaaatttc tgaagggttt ctgaaacccg ttagagaaga acacatacca tggtaaactt
    11401 tagaaatcgc attgcttggg taacaggggc ctcgtcaggc ataggcaagg agattgccct
    11461 ggcacttgca agggaacagg cacatcttat actgtcagcc aaaaatcctg aggagcttga
    11521 ggttgtggca tccgaatgcc gcaagtacac ccctttttgc catgaggttc cttttgactt
    11581 atctgatcct gaagaagttg agcgcattgc aaaatctgtg gttaacacct atggtcatat
    11641 tttcctgctg attaataatg gtggaattag ccaacgctcg ttggctgttg aaacccccgt
    11701 tgaaatagac cgaaaaatta tggaggtcga ttacttttct catgtcatta ttactaaaac
    11761 tgttatacca ggaatgattg aagcgggtgt ggggtatatt gcagcaacga gcagtatttc
    11821 aggaaaattc ggattcatgt atcgttcagc ttatgctgca gccaaacacg ccattcaggg
    11881 tttttttgaa acgcttaggg tagagcttaa gcctcacaac atttcggtaa ctattgccta
    11941 cccgggccga atccggacca gtatttccct tcacgccctg gataaggagg gtaacgagta
    12001 tggaaaaatg gaccccgggc agagcaacgg cctggcaccg caaaannnnn nnnnnnnnnn
    12061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12121 nnnnnnnnnn nnnnnnnnnn nnnnngtaac gagtatggaa aaatggaccc cgggcagagc
    12181 aacggcctgg caccgcaaaa atgtgccaaa aggtatatcc gtgctgtaaa gcggcgtaaa
    12241 ccggaggttt tgattggaaa aagcgagctg ctgatggttc acattaaacg tttactgcca
    12301 tcgctattct ataaaatggt tagcagggta aaacctgtgt aaaagttaac attcatggag
    12361 aaaattattt acggcattca gcaggttggc ctaggggtga gcaacctgga agaagtgtgg
    12421 aaatggtaca tcgattcctt aggatttgac gttaaaattt ttgacgacga cacggtagcc
    12481 gagctaatgc tcccctacac tggtggtgaa ccccagcagc ggcacgcaat tcttgccctg
    12541 aacctccagg gaggcggtgg cttggagatt tggcagtaca aggagcggac tcctcttcct
    12601 ccaaagttcc cggtaaaact tggcgacctg ggaatttttg ctgctaaaat taaaagtccc
    12661 gatatcgagg ctgcctttaa ctttctgaaa tccaaaggcg caacacttgg cgatttggtt
    12721 aattctcctg aggggaagcc acatttctac atatccgacc cctttggcaa taccctggag
    12781 gtttacgagg acaaatcaac ctttttaaat gagggaagaa gcacttccgg gattggcgga
    12841 gccattgttg gggtaagcca gatggagagg tcggtaaaat tttactcgag agtgctgggc
    12901 tacgacaagg ttgtttacaa taaatcaggc aggttcgatg accttgccgg ccttcctgcc
    12961 ggaaacgaga attttcaccg ggtgctactt acccattcgg atacccgcaa gggcagcttt
    13021 agcgagctat ttggccaatc gtacattgag cttattcaac cgctggatag agttccattg
    13081 aaaatcctgc aggaccgttt ctggggtgac cttggcttta ttcacctttg ctttgatgta
    13141 aagaacatgg accagctcga acgggagtgt aacgagctgg gttttccctt cactgtcaac
    13201 tcaaatatca agcatagcga taagggtagt tttgatatgg gcgaggctgc aggccatttc
    13261 acctacattg aggatcctga cgaaactctg attgagtttg tcgaaaccca cttcctgcca
    13321 atatataaac cgctaggtat aaaggtgaat tttcaaaacc gcaggcccta caagtctgtt
    13381 ccccgatgga tgattaagct gcttcgtttt aaaagggtca agaagattta atggctgtct
    13441 attgttgcaa ccatttttcc caaactacac cttaaaccct aaatgattac catgaacttg
    13501 gggtgcaggg tttaacctta accctatagg ttttagctat cacgctggct taaatatgtt
    13561 tcaaggggac accggtagag caaatgaaat atccttttat ggattttttt agctgtgatt
    13621 aaccagtaga ttatgcgtag ttgcgcaatc agtatgttga gtttagtatt ttagaatgct
    13681 gaagtagaaa gttaacggta tccccttgca cgagctttgc tgctttcctg tttagcttaa
    13741 acacaaaacg ccaggatttt tattcctgct atagagggta gaattatgtt ccttaacata
    13801 ttccacccaa gggcaggcta tttgaaaatt gacaaactag tagtaacctt gcatacctta
    13861 aaaaagacaa tgatttttaa gcaaaatatt gtttaacccg taattcatag taaaaatgag
    13921 taagatgaca ccaaaagatt acatggatcg tgagagcaag tacggtgctc acaactacca
    13981 tccgcttccg gttgttttgg aaaaaggtaa gggggtttac ctttgggacg tggagggaaa
    14041 gaagtactac gattttcttt cggcctactc tgctgttaac caggggcatt gccacccaaa
    14101 aattgttaaa gcgttaaccg accaggccca gctgcttacc cttacctcaa gggctttcta
    14161 caacaacgtc ctcggtgagt ttgaggagta cgttaccaaa tactttggct atgacaaggt
    14221 actgccgatg aatacagggg ctgaaggcga tgaaacagct ctcaaacttt gccgccgttg
    14281 ggcatacgat gtgaagggaa taaaggatgg cgaggccaag attattgttt gcgagaataa
    14341 ctttcatggc cgcaccataa ccgtgatttc catgtcaacc gatcccgatg cctaccgcgg
    14401 gtacggcccc tacacccccg gttttattaa aattccgttc aacaacatac ctgcccttga
    14461 ggatgccctt aaggataaaa acgttgccgg attcctggta gagccaatac agggcgaagc
    14521 gggagtgttt gttcctgacg atggttacct caaaaaagcc tatgagctct gcaaaaagca
    14581 caatgtgctg tttattgccg acgaggttca aaccggcatt gctcgcaccg gaaagatgct
    14641 ggcttgcgac catgaaggtg ttcgccccga tattttgata ctcggaaaag ctatatcggg
    14701 aggtgttgtt ccggtttcgt gcgttctggc cgatgatgag attatgctta ccattaagcc
    14761 gggtgaacat ggctcaacct acggcggaaa ccccttggct tgcaaggttt ctattgctgc
    14821 acttgaagtg attaaaaacg aaaagctggt agagaatgcc gcaaaaatgg gggaaatatt
    14881 ccgtagagag atgaggaaca tccagagcga catgattgag ctggttcgcg gcaaggggct
    14941 gcttaacgca gttgtaatcc gcaacaagcc cggcaaaaca gcatgggacg tttgtttggc
    15001 tatggccgat aagggcgtat tggccaaacc tacacatggc aacattatac ggtttgcccc
    15061 accattggtt ataaccgaga aggagcttct cgacgccatt agccggatta aggaagcttt
    15121 taagctcttc gagtaaccgc taattgttgg caaaagcatg gaagcgcctc ctgagggagg
    15181 cgcttttttt gtagaatatg ccataccagg atggtggaac gccattggaa tgtaagttta
    15241 tttacccaat atcctgacaa tatgcttatt gagctagcat gagattcaat attgccgact
    15301 ttttgttcgt tggttgatgt aagtaagcct atttaatgtc ttcgtttttg ccattttatt
    15361 catttaaatg gcatcctgct ccagttgcct aatttttacc cagagatact tgtttttgaa
    15421 agcctaattt tgtctataat aatttttgac acgggatagt attgtaaaaa tgtggtaatg
    15481 gaatgacggg gccaactcat gagtttcctt tctaccatct aaaaaatgcc ttacaccgat
    15541 ggcctgatga tattcgtgta atcgaagttg actccttagc tgtagggtgg taacttactt
    15601 aaaaaacatg accccaatta
//
LOCUS       DAOSHM010000055        15485 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_3125_length_15485_cov_19.766494, whole genome shotgun
            sequence.
ACCESSION   DAOSHM010000055 DAOSHM010000000
VERSION     DAOSHM010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 15485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15485
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3125_length_15485_cov_19.766494"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            203..1438
                     /locus_tag="PK839_08385"
     CDS             203..1438
                     /locus_tag="PK839_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOG72790.1"
                     /translation="MIEFSRHILPNGLTLIVHQDFSTPIAAVNMLYKVGSKHEHAEKT
                     GFAHLFEHLMFGGSENIPVYDEPLTAASGENNAFTNNDITNYYITLPAENIETAFWLE
                     SDRMLSLAFSEQSLNVQKKVVVEEFNQHYLNQPYGDVWLLLRPLAYAVHPYSWPTIGK
                     SPEHIQNATLIDVKDFFYHHYAPNNAILTIAGPVEPESTVELVQKWFGEIERRALDDK
                     NIPVEPKQESPRFLEVSRNVPFNAIYKAYHICRRNHPDFAPTDLISDILSNGKSARLY
                     QHLVKEKQLFSNINAFVTGDIDEGLLVVTGQLFPSTSFRDADESITSELEELKIKPVS
                     DYELEKVKNRFESNFVFEETSILNKAINLSFYEQLGNAGYINQEVEKYRAVSTNRIME
                     VAREILSVNNCSTLYYKKN"
     gene            1453..2754
                     /locus_tag="PK839_08390"
     CDS             1453..2754
                     /locus_tag="PK839_08390"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOG72791.1"
                     /translation="MTLSKLNRKEPPERRAVRKIELPEPSISTLPNGAKLAVINAGTQ
                     DIVKMEVIFNGGTRYQKKILSSQAAVSLMCEGTTTRNSNQLAEQLDFLGSFLQHSFDR
                     DFASVSLFTTRKHFAASLEVVADILTNPTYPENELEIFRKKGKQSLTVELEKVETLAK
                     QGFFQCLFGPDHPYGSFATPDDFDKLSQPDVADFHKNFHNLSNSFIILAGRVDEHEVK
                     TVETLLGANSPGRRLGALNNSLPAAAQTPREKFTYKENAIQSAVRIGKQMVSRNHPDY
                     IGLMVLNTILGGYFGSRLMQNLREDKGFTYGISSILIPYRETSVFMIGTEVGREVTKP
                     AVEEIYNELDKLVRVPVPAEELELVKSQMTGDLLRTLDGPLAIADSIAELYQYNNMNL
                     NFLDQTIETINTITSNKLQSLAISYFNPDEMVKSVAGAQPF"
     gene            2813..4411
                     /locus_tag="PK839_08395"
     CDS             2813..4411
                     /locus_tag="PK839_08395"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOG72792.1"
                     /translation="MGKVKSTSVKLLTAILLMTAGQAAAQMYDKPKEPTFDYMTINIS
                     TGNPTLFWTPPSPDPQYPNPEGYIIYKHFVDILGNDFYEAIDTVDSSTFSYVDLTSNG
                     NLERLFYRIASKGPTEPSRQTAEHGQIWLTSSYDSCNAKIDLTWMHYAGWRNIDINQN
                     LKLYMSHNPDPSTFELIAEIDKYNSVYSITNVGENLDYYFYMTSGRIDKPFTTFSNLH
                     HIHTKMAKHPDYMTIDSVISVANGNAVYYSIDPTTEIRRFKVIRWEQPDTNKSIFSAK
                     VIEEFTDPAKNTTTDSTDSWAARTRKFYYKVDAYNGCSDAVKVTNLCNTIIPHVKPSG
                     KKVLLSWDTLMVDTYRQPDRINDWVEYTVYRRAYTSSSDLNGPGELSVAAQGLTEPSF
                     EDDLSAFQGHDPLYTITFKYYVEAIERSTYSTFCRSREIATEILPGATLPTAIDPTDP
                     TSINGHPRNIFQPVISFDASYNLTIYDRWGGVVYHGNQGWDGRNSSGEFVKEGTYIYR
                     LEINTSNAGKVVKNGSVSVVFPKH"
     gene            4597..6831
                     /locus_tag="PK839_08400"
     CDS             4597..6831
                     /locus_tag="PK839_08400"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HOG72793.1"
                     /translation="MSLQTEEEKLLIQSHFEDLLRNCPRCSSEEDIKLITKAFNLANE
                     AHKGVRRKSGEPYILHPIAVAKIVSQEIGLGAKAIACALLHDVVEDTDYTIEDIERIF
                     GKKIASIIDGLTKITEVFDANSTIQAENFRKILLTLADDIRVILIKLADRLHNMRTLE
                     SLPATKQMKIASETIYLYAPLAHRLGLYSIKTELEDLSLKYRFPQVYEEIQSKIELNE
                     KRRVQLINHFSLPIIKKLEENNIDFEISGRPKSVYSIWKKMQTKNVSFEEIYDLLAVR
                     IVFNPYPHIPEKTQCWHIYSLITDIYKPKPDRLRDWVSTPKANGYEALHCTVMGPNGQ
                     WVEVQIRSQRMDEIAERGFAAHWKYKDMDNASHENELDKWIKRVREMLENPQENALEF
                     LDEFKVNLFTSEIIIFTPKGETRAMPKGSTALDFAYEIHTEVGNKAIGAKVNHKLVPL
                     SYILSSGDQVEIITAASQKPSWELLKYVTTAKAKNAIKNALKTETKDRLEKGKKLLDD
                     RLVELGVQPSSKILKKVLTSYEVSSKDELYSKIGSGQVNLDNLAKILKKNTKNKWIKY
                     WQLQLGRKSHPQNEPATLQKIDTKKPFLLSENVDSENPPYVIARCCNPIPGDDVIGYI
                     SPDDVVIIHKATCAEAIRLMAQHGDRIVSAKWTTHKVLSFLARIEVKGMDRIGIINDL
                     TRVISSELMVNIRKFHIESHDGIFEGFIDLYVHDISHLNNLIFNLMKLKGMDNVKRVD
                     KFDD"
     gene            6926..7399
                     /locus_tag="PK839_08405"
     CDS             6926..7399
                     /locus_tag="PK839_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HOG72794.1"
                     /translation="MGCTETKTVVKQILSDYLQRKGYRETPERYAILQEIYSTDSHFD
                     VETLYIHMKNNGYQVSRATIYNNIDLLCECGLVIRHQFGKKNSLYEKAYECKQHDHLI
                     CTRCGKVTEFCDPRIQEIINSAKRNSNFTISHHSLYIYGICAECRNEIEKVPITL"
     gene            7610..8878
                     /locus_tag="PK839_08410"
     CDS             7610..8878
                     /locus_tag="PK839_08410"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HOG72795.1"
                     /translation="MTKVDVLLGLQWGDEGKGKVVDVLTPNYDAIARFQGGPNAGHSI
                     EFNSIKHVLHTIPSGIFHPSCINIIGNGVVLDPIVFAEEVEILTKMNVDLHKTLLISK
                     KAHLILPSHRELDAASEAAKGKGKIGSTLRGIGPTYMDKTGRNGLRVGDILSPDFTRN
                     YEFLKNKHKELLQQYTYQSNIEQHEDAWFKGVEVIKQFRLVDSEYDINEMLVAGKRVL
                     AEGAQGSLLDIDFGSYPFVTSSNTICAGACTGLGVSPHSIGEVYGIFKAYCTRVGSGP
                     FPTEQENDTGEKMRNLGVEFGATTGRPRRCGWLDMVALKYAIMLNGVTRLIMTKADVL
                     DSFDTIRVAVAYLVNGKQTTRLPYDINVPVEPVYKEFKGWNSDITGITNEQDLPVELA
                     EYIRFIEHETGVPIHIVSVGPNRDSTIVRA"
     gene            complement(8894..9562)
                     /locus_tag="PK839_08415"
     CDS             complement(8894..9562)
                     /locus_tag="PK839_08415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72796.1"
                     /translation="MKRSAFFILVHLAAIFSCRAQGKVETDSSATMSIVRYATDLRCL
                     DGENAELLVNVLYEFNGLPPINRIELELPEAKEKVFRQIFVEVSSNDKKFIFRRKIRK
                     PDADELSALDPCALASKYSNLKRPVTVEWEIRVSLRSPAGSYKWPSIATGNFERIDHL
                     SFRVAYGKELSADIQTNLTTDPQKDTINGNFYYLWEFNDVNPSRTELKGFHSHDPYVI
                     LNFR"
     gene            complement(9636..11036)
                     /locus_tag="PK839_08420"
     CDS             complement(9636..11036)
                     /locus_tag="PK839_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOG72797.1"
                     /translation="MEKIRETLRDSAVARWTALALVAFTMLTGYYLTDVMAPLKGLLE
                     EQLTWNSTEYGIFTSAYGWFNVFLLMLIFGGIILDKMGVRVTGLGAAAVMITGTAIKF
                     WAVSTHSLDGQVWNILWLFPMKAQVFMAALGYAVFGVGVEVAGITVSKIIVKWFKGKE
                     LALAMGLEMATARMGTALALATSVPIAKSLGHVSKPILVCLIMLCIGFISFFIFTFMD
                     RKLDASQAAYDKDNNVQNDEEAFKVRDIFKIITNRGWWYIAILCVLFYSAVFPFLKYA
                     SDLMVNKFGISENLAGTIPAMLPFGTILLTPLFGNLYDRKGKGATIMLIGALLLILVH
                     SFFSLPFLTHWIVAIILIIILGIGFSLVPSAMWPSVPKIIPERQLGTAYSLIFWVQNW
                     GLMGVPALIGWVLDKYCVTGERITDSGLTVHTYNYTIPMLIFTSLGILALVFAFLLKA
                     EDKKKGYGLELPNIAK"
     gene            complement(11203..11667)
                     /locus_tag="PK839_08425"
     CDS             complement(11203..11667)
                     /locus_tag="PK839_08425"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOG72798.1"
                     /translation="MKKLTCVLILLTLLGSGCKFVEKYTKKGKKAKAEAMQKAKEDSL
                     AKAKAIEFEKAALAREQAHQDSIRQVEEYENQHRFHVIIGSFKVPSNATSWEQEVRGM
                     GFNNTRILQAPNGFNLVSVGAFNTYSKAFNEIDRINSNRIENPMEMWVYEKQ"
     gene            complement(11885..12733)
                     /locus_tag="PK839_08430"
     CDS             complement(11885..12733)
                     /locus_tag="PK839_08430"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOG72799.1"
                     /translation="MVRGICKTPDTPLVSSVVVHRGEEPVLSGEQGVCNVFFAHCNLQ
                     CSYCQNHQISRNSITNREWKMSVNEVAEEICGLLNRGISMLGFVSPSHQVVQMLEILE
                     LVRREGYSPRIIYNSNGYDSVETLRQLEGIVDIYLPDFKYVNPELGLRYSGVADYPDV
                     AAKAIREMYRQKGSSLLVDNDGLAESGMIIRHLVLPGYADESIRLLKFLAEEFTPRIY
                     LSLMSQYYPPVGVELQSPLNRTVTMEEYMGVVEVAQGIGFRGWIQDLESFGSYQPDFS
                     RSNPFE"
     gene            13328..15235
                     /locus_tag="PK839_08435"
     CDS             13328..15235
                     /locus_tag="PK839_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014776519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOG72800.1"
                     /translation="MKTIINLFEESVTKYPDNACIWEKLTDKYESSSYSEVRKEVYRL
                     AAGFLSLGIGKNDKVALLSEGRKLWITSELAILYTGAVNVPLSVKLDGNDLLFRLVHS
                     ETSTIIVSGIQAPKITRIKKEIPNLKRIIYLDSLNQYSEGEMGIEEVFKLGDTFLAEH
                     PGRVEEVSSSVKPSDFANISYTSGTTADPKGIILSHNNYTANVEQALTLMDIPQSYRT
                     LLILPLDHCFAHVAGVYAFMAMGASAATVQVGKSAIDTLKNIPINIKEIQPSLLLSVP
                     SLAKNFKKNIEVGIREKGKFTEKLFNNALKVSYRYNKEGWNKGNNFTFIYKPLIWIYD
                     KLIFSKVREVFGGRLEFFIGGGALLDMELQRFFYAIGIPMMQGYGLSEATPIISSNSL
                     KRHKLGSSGYLVKPLDLKICDESGNELPVGQKGEIVVRGENVMVGYWKNEKATADTIR
                     NGWLHTGDMGYMDKDGFLYVLGRFKSLLISSDGEKYSPEGIEGALEEHSKFIENVILY
                     NNQNPYTIALIVPSKESIRRALNEKGIDMNTHEAGIEAIKLINNEVSRFKTGGDLQNL
                     FPERWLPSAFSILEEPFTEDNGLINSTMKVVRGKVEEKYRDKIEHLYTVEGKNIYNNS
                     NISSMLKIISN"
BASE COUNT         4669 a         3706 c         3224 g         3886 t
ORIGIN      
        1 atttcacccc atttgcctta tggctcaaat caccccagca aactttacgt caaagttctg
       61 caacaacttt gaaaaattgg ctacttttga aacctcttta tcctaaggca taaaacttta
      121 gcagctatcg caggcaacat cctagccatc tgcctctcga ggttgccctt ttgctgacat
      181 tagaagttaa caaccaacta ttatgataga gttttcacga catattttac cgaacgggtt
      241 aacgctgatt gttcatcagg atttcagcac cccgatagct gcagtaaaca tgctctacaa
      301 agtaggttca aagcatgaac atgccgaaaa aacaggcttt gcacatcttt tcgagcattt
      361 gatgtttggc ggttcggaaa acattcccgt ttacgacgaa ccgctaaccg cagcaagcgg
      421 agagaacaac gccttcacca acaacgacat caccaactac tacataaccc tgccagctga
      481 aaatattgaa acagcattct ggctcgaaag tgaccgtatg ctatcacttg ccttttcgga
      541 gcaaagccta aacgttcaga aaaaggttgt agttgaggag ttcaaccagc actaccttaa
      601 ccagccatat ggagacgttt ggttgctact tcgccccttg gcttacgcgg tacatcccta
      661 cagctggcct acaattggta aatcgcccga gcatatccaa aacgcaaccc ttattgacgt
      721 taaagatttt ttctaccacc actacgcccc gaacaatgcc attcttacaa ttgccggacc
      781 agttgaaccc gaatctactg ttgaactcgt ccagaaatgg tttggggaaa ttgaaaggcg
      841 agccctggat gacaagaata ttccagttga acctaagcaa gagtcacccc gttttctgga
      901 agtaagccgc aatgtcccct tcaacgctat ctacaaagct taccatatct gccgacgaaa
      961 ccatcctgat tttgcaccta ccgacctgat atcggatatt ctttcaaatg gcaagtcggc
     1021 tcggctctac cagcacctgg taaaggaaaa gcaactcttc agcaacatta atgcctttgt
     1081 taccggcgat attgacgagg ggctgctggt tgttaccggg caactctttc cctctacatc
     1141 ttttagggat gcagatgaaa gcataacctc cgagctggaa gaactcaaaa tcaagcctgt
     1201 ttctgactat gagcttgaaa aggttaagaa ccgctttgaa tccaacttcg tttttgaaga
     1261 aaccagtatt ctgaacaagg ccattaacct ctcgttctac gagcaacttg gcaatgccgg
     1321 ctatataaat caggaggtag aaaaatatag agcggttagc acaaaccgga ttatggaggt
     1381 tgcgcgggag atactttccg taaataattg ctccacccta tactataaga agaactaaac
     1441 atcaaaatat aaatgaccct cagcaagcta aacaggaaag agcctccaga aagaagagct
     1501 gttaggaaaa tagaactacc tgaaccgtcc atcagcaccc tgcccaacgg tgctaaactg
     1561 gcggtaataa atgcgggtac ccaggatatt gtcaaaatgg aagttatttt taatggtggc
     1621 acccgatacc agaaaaaaat tctatcgtca caggcagccg tttcgttgat gtgcgagggg
     1681 acaaccaccc gaaattcaaa ccagcttgca gaacagctcg actttttggg tagttttctg
     1741 caacattcat ttgatcgcga ctttgcgtcg gtatcgctat ttaccacccg taaacacttt
     1801 gccgcttcac tcgaggtagt agctgacatc ctaaccaatc ccacataccc ggagaatgaa
     1861 ctcgaaattt ttagaaagaa gggaaaacag agccttactg tggagctgga aaaagttgaa
     1921 acactggcta agcagggttt ctttcagtgt ctttttggtc ccgaccatcc ctacggcagc
     1981 tttgccaccc cggacgattt cgacaagcta tcccagcctg atgtggccga ttttcataag
     2041 aacttccaca acctttccaa cagcttcatc attctggccg gaagagttga tgagcatgaa
     2101 gtgaagaccg ttgaaacact tctgggagcc aattcaccgg gaagacgttt aggggctttg
     2161 aacaattctt tacccgcagc tgcacaaacc cccagagaaa aattcacgta taaagaaaat
     2221 gccattcagt cggcagtaag aattggaaaa caaatggtta gccgaaatca tcccgattac
     2281 attgggctta tggttctaaa cacgattctg ggtggatact ttggctcaag gcttatgcag
     2341 aaccttcgag aggacaaggg attcacttac ggcatttcat ccattctgat tccctacagg
     2401 gaaacatccg tatttatgat tggcacagag gtaggcaggg aagttaccaa acctgccgtt
     2461 gaggagattt acaatgaact tgacaagctg gttcgtgttc cggtacccgc tgaagagctg
     2521 gaactggtaa aaagccagat gactggagat ctgcttcgaa ccctggatgg cccactagct
     2581 attgccgaca gcattgccga actctaccag tacaacaaca tgaatttaaa tttccttgac
     2641 caaactattg aaaccattaa caccatcacc tccaacaagc tgcaaagcct ggctattagc
     2701 tacttcaacc ctgacgaaat ggtaaaaagt gtagctggtg cgcagccttt ctgaaaccaa
     2761 aacttgaatt taacagctaa ttttcggttt ttaaccgcta acaataaagt caatgggtaa
     2821 ggtaaaatca acgtcagtaa aactacttac agctatcctg ttgatgactg ctggacaagc
     2881 agctgcgcag atgtacgata agcccaagga accaaccttc gactacatga ctataaacat
     2941 aagcacgggt aaccccacac ttttctggac tcctccctcg cccgatcccc agtatcctaa
     3001 tcctgagggc tacattatct acaagcattt tgttgacatt cttggaaacg atttctatga
     3061 ggcaattgac actgttgata gcagcacatt ctcctacgtc gatttaacct ccaacggcaa
     3121 tcttgaaaga ctattctacc gcatagcttc gaaggggccc actgagccaa gcaggcaaac
     3181 tgccgaacat ggccaaatat ggctgacgtc cagctacgat agctgcaacg caaaaattga
     3241 cctgacctgg atgcattatg ccggttggag aaatattgat atcaatcaga accttaagct
     3301 ctacatgagc cataacccgg acccttctac ctttgaactc atcgccgaaa ttgacaagta
     3361 caactccgtt tacagcatta ccaacgtggg cgaaaacctc gactactact tttacatgac
     3421 atcgggcagg atagacaaac ccttcacaac cttctcaaac ctgcaccaca tacacaccaa
     3481 aatggccaaa catcccgact acatgaccat tgactcggta atttcggtgg ctaacgggaa
     3541 tgccgtttac tactccattg acccaacaac tgaaatcaga agatttaagg ttatacgatg
     3601 ggagcaaccc gacaccaaca agagtatttt tagcgcaaag gtgatagagg agttcaccga
     3661 ccctgcaaaa aatacaacca cagacagcac tgacagctgg gcagcacgca cccggaaatt
     3721 ctactacaag gttgatgcct acaacggatg cagcgatgcg gttaaggtta ccaacctatg
     3781 caacaccatc atcccccacg taaagcctag cgggaaaaaa gtgctgctaa gctgggatac
     3841 cctgatggtt gacacctacc gtcaacccga caggataaac gactgggttg aatataccgt
     3901 ttatcgaagg gcctacacct ccagcagcga tttgaatggg ccaggagagt tgagcgttgc
     3961 agcccaaggc ctaaccgaac catcattcga ggatgatcta tcggcctttc agggacacga
     4021 ccccctctat accatcacct tcaagtacta cgttgaggcc attgagcgga gcacttacag
     4081 cactttctgc cggtcgcgtg aaattgccac cgaaatactc cccggagcta cacttcccac
     4141 cgccattgac ccaaccgacc caacttcgat aaacggacat ccccgtaaca ttttccaacc
     4201 ggttattagc tttgatgcca gctacaacct taccatttac gaccgatggg gaggcgttgt
     4261 gtaccatggc aaccagggct gggacggtcg caattccagc ggggagttcg tgaaggaggg
     4321 aacctacatt taccggctgg aaattaacac cagcaacgct gggaaagttg taaaaaatgg
     4381 tagcgtttcg gtggtatttc ctaaacatta agatgaactt ccccaatggc aaaatgtttt
     4441 acattaaaag agctgatttt ccttagggga aaaaatctcc ttccacagta ctttccaccc
     4501 gggaaaaagc ggataagcct tatttttaaa tacacaccca tcaccacaac ctgttaaaaa
     4561 acattcatca aaatttccaa acaactccat gctaccatga gccttcaaac cgaagaggaa
     4621 aaactgctca tccaatcaca cttcgaggat ctcttacgga actgcccgcg atgtagctcc
     4681 gaagaggata taaagttgat taccaaagcc tttaatttgg ccaacgaagc tcataagggt
     4741 gttcgcagaa agtcgggcga accctacatt ctacacccca ttgcagtagc caaaatagtt
     4801 tcacaggaaa ttggacttgg agctaaagcc attgcctgcg cgttgctcca cgatgtagtt
     4861 gaggacaccg actacaccat agaggatatc gagagaattt tcgggaaaaa aattgcctcc
     4921 atcatcgacg ggctaacaaa gataaccgag gtttttgacg caaactctac catccaggct
     4981 gaaaattttc gcaaaatact ccttaccctg gccgacgaca tcagggttat cctcattaag
     5041 ctggccgata ggctccacaa catgcgaacg ctcgagtcgc tgccagctac caagcagatg
     5101 aagattgcca gcgaaaccat ctacctttac gccccgttgg cacaccgtct tgggctttac
     5161 tccattaaaa ccgagctaga ggatctcagc ctgaagtacc gctttcccca ggtgtacgag
     5221 gaaatccaaa gcaaaataga gctgaacgag aaacgcaggg ttcagctcat taaccacttt
     5281 tcgctaccca tcattaagaa gctggaggaa aacaacattg actttgagat aagcggtcgt
     5341 ccaaaatcgg tttactccat ttggaagaag atgcagacca aaaacgtttc cttcgaagaa
     5401 atatacgacc tactggcagt gcggattgtt tttaacccct acccccacat tccggagaaa
     5461 actcaatgct ggcacatcta ctcattaatt acagatatct ataaacccaa gcccgaccgg
     5521 cttcgcgatt gggttagcac tcccaaggcg aacggctacg aggctctcca ctgcacggtt
     5581 atgggaccta acggacagtg ggtggaagtt cagatccgct cccaacgaat ggatgaaatt
     5641 gccgaacggg gatttgctgc ccattggaag tataaggaca tggacaatgc cagccatgag
     5701 aacgagctcg ataaatggat taagcgggtt agggagatgc ttgagaatcc ccaggaaaac
     5761 gccctcgagt ttctcgacga gtttaaggtc aaccttttta cctctgaaat catcattttc
     5821 accccaaaag gcgaaacccg tgcaatgccc aaaggctcta ctgccctcga ttttgcctac
     5881 gaaattcata ccgaggttgg caataaggcc attggtgcta aggtgaacca caagctggtg
     5941 ccgctaagct acatcctttc cagcggtgac caggttgaaa ttattaccgc tgcttcacaa
     6001 aaaccctctt gggaattact aaaatacgtt acaactgcca aggccaaaaa tgccattaaa
     6061 aatgctctta aaacagaaac caaagaccga cttgagaagg ggaagaagct gctcgacgac
     6121 aggctggttg agctaggcgt ccagccttcc tcaaaaatat taaagaaggt gctcaccagc
     6181 tacgaggtgt catcaaaaga tgaactctac agcaaaatag ggtccggtca ggtcaacctc
     6241 gacaacctcg ctaaaatcct gaagaaaaac accaagaata aatggataaa gtactggcag
     6301 ctccagctgg ggagaaagag ccacccccaa aatgaacctg ccacgctaca gaaaatcgat
     6361 accaaaaagc ctttccttct cagcgaaaac gttgactccg agaaccctcc ctatgtgatt
     6421 gcccgctgct gcaatcctat acccggcgat gatgttattg gctacatttc gcccgacgat
     6481 gtggtaatca tccacaaggc cacttgtgcc gaagccatcc gccttatggc ccagcatggc
     6541 gatagaattg tcagcgccaa atggacaacc cacaaggttc tctcatttct cgcccgtatt
     6601 gaggttaagg ggatggatag aataggcatt atcaacgacc ttaccagggt catatccagt
     6661 gagctgatgg tcaacatccg taagttccac atcgaaagtc acgatgggat ttttgaaggg
     6721 ttcatagatc tttatgtcca tgacatatcc cacctcaaca acctcatatt caaccttatg
     6781 aaacttaaag ggatggacaa cgtcaaacgg gttgataaat ttgacgacta aacaaaaata
     6841 ctaaccagct gttaaccaac ttacaaatca agctgctaat aggataaatt tattgtctca
     6901 ttccgtataa cttaaaaagc acgccatggg ctgcacagaa accaaaactg ttgtaaagca
     6961 aatactttcc gactacctac agcgcaaggg gtaccgtgaa acccccgagc ggtacgccat
     7021 actgcaggaa atatattcca ccgatagcca tttcgacgtg gaaacccttt acatccacat
     7081 gaaaaacaac ggataccagg taagccgggc taccatttac aacaacatcg acttgctatg
     7141 cgaatgcggg ttggttatca ggcatcagtt tggaaagaaa aactcccttt acgaaaaggc
     7201 ttacgagtgc aagcagcacg atcacctaat ctgtacacgc tgcgggaagg ttaccgagtt
     7261 ttgcgacccc agaattcagg aaataatcaa ctcggcaaaa cgaaactcca acttcaccat
     7321 ctctcaccac tcgctttaca tatacggcat ttgtgccgaa tgtcgtaacg aaatcgaaaa
     7381 agttccaatc accctgtaac gctcccttgc cgggtatgtg tcatcacggc aagcctgggc
     7441 tatggttgtc ttttccacat tactaattcc aacagccaac cccattacca gaatccactc
     7501 cgtattaaaa ttggtgctgt ttaaaacttt tacagcgcca tttacctaaa ttttgtaact
     7561 ttaaaaggtt aaattttatg atcgattcca taatcttttc atataagcta tgacaaaagt
     7621 tgatgtactg ctagggttac agtggggcga cgaggggaag ggcaaggtag ttgatgtgct
     7681 tacccctaac tatgatgcaa tagcacgctt tcagggaggc ccaaatgccg gccattcaat
     7741 tgagttcaac agcatcaagc atgtgctgca cacaattccc tcgggaatat tccacccttc
     7801 atgcattaac atcataggga acggggtggt gctcgacccc attgtttttg ccgaggaggt
     7861 tgaaattcta actaaaatga atgttgacct ccataaaacc ctgctaattt caaaaaaggc
     7921 ccacctgata ctcccctccc accgcgaatt agacgcagct tccgaagccg ctaaaggaaa
     7981 gggtaaaatt ggctctaccc ttagaggcat tggccccaca tatatggaca aaaccggccg
     8041 caatggcctt agagtaggcg acattctttc ccctgatttt accagaaact acgagttttt
     8101 aaaaaataag cataaggaac ttctccagca gtacacatac cagagcaata tcgagcagca
     8161 cgaggatgct tggtttaagg gcgttgaggt gataaaacag tttcggctgg ttgactcgga
     8221 gtatgatatt aacgaaatgc tggtcgctgg taaacgggtg cttgctgagg gcgctcaggg
     8281 ttcgctcctc gatattgatt ttggttcgta cccattcgtt acctcctcaa ataccatttg
     8341 cgccggtgca tgcaccggtt taggagtttc gccccatagc attggggagg tttatggaat
     8401 ttttaaggca tactgcaccc gggttggctc agggcctttc cctaccgagc aggaaaatga
     8461 taccggggaa aagatgcgta acctgggcgt agagtttggt gccacaaccg gacgtcctcg
     8521 acgttgcggc tggctcgata tggttgccct caagtatgcc atcatgctga atggagttac
     8581 ccggctcatc atgactaaag ctgatgtact cgattcgttc gacaccatca gggttgccgt
     8641 agcttaccta gtcaacggta agcaaacaac ccggttaccc tacgatatca atgttccggt
     8701 tgaacctgtt tacaaggaat ttaagggctg gaatagcgat atcacaggaa taaccaacga
     8761 acaagattta cctgttgagc tggcagagta tattcgtttc atcgagcatg aaacaggagt
     8821 tcctatacac attgtttcgg taggccccaa cagggattct accattgtta gagcctgacc
     8881 aacccttctt ttgttagcga aaatttagga taacatacgg atcgtggctg tgaaagcctt
     8941 tcaactccgt acgtgaaggg ttaacatcat taaactccca gaggtagtaa aaattcccat
     9001 ttatagtatc cttctgggga tcagtggtaa ggttggtttg aatgtcggca gaaagttcct
     9061 tgccgtatgc taccctgaag ctaaggtggt ctatcctctc aaagttaccc gtagcaattg
     9121 atggccattt gtagctgcct gctggtgagc gaaggcttac tcttatttcc cactccacgg
     9181 ttacaggcct ttttaaatta ctgtactttg aagccaatgc gcaggggtcg agggcggata
     9241 actcatcggc atcgggcttg cgaatttttc ttcgaaaaat aaacttctta tcattactgc
     9301 ttacctctac gaaaatttgg cgaaacacct tctctttagc ctcgggcagc tccagctcaa
     9361 ttctgtttat ggggggcagg ccgttgaatt cgtagagaac gttcaccagc agttcggcat
     9421 tctctccatc taaacatctt aaatcggtag catagcgaac tatgctcatg gtagcggagc
     9481 tgtcggtttc aacttttccc tgagcccgac aagaaaaaat cgctgccagg tgaacaagta
     9541 taaaaaatgc agatcgcttc attaaacttt tctaatatgc gttaaaacgc ccttgttggg
     9601 ggcgtttcaa cgcattagtc cattacataa gctgactact tagctatatt agggagttcc
     9661 agaccgtaac cctttttctt gtcctctgct ttaagcagaa aagcaaacac caacgccaga
     9721 attccgagcg aagtaaatat cagcatggga atggtgtagt tgtaagtatg gacagtaagg
     9781 ccactgtcag taattctctc tccggttaca cagtacttgt cgagcaccca gccaattaaa
     9841 gcaggaacgc ccatcaaccc ccagttttga acccaaaaaa tcaaggagta agctgtgcca
     9901 agctgacgtt cgggtataat tttgggtaca gatggccaca tggccgaggg caccagcgaa
     9961 aatcctattc ccagaatgat tataaggatg atggccacta tccagtgagt aaggaatggt
    10021 aatgagaaaa aggaatgaac caggataaga agtagggcgc caatcagcat aatagtagca
    10081 cctttcccct ttcggtcgta taagttaccg aagagagggg taagcagaat tgttccgaat
    10141 ggaagcatgg ctggaatggt gccggcaaga ttttcgctaa ttccaaactt attgaccata
    10201 aggtccgacg catatttaag gaatgggaaa acagcggagt agaaaagcac acaaagaatg
    10261 gcaatatacc accacccgcg gttagtaatt attttgaaaa tatcacgaac cttaaatgcc
    10321 tcctcatcgt tctgcacgtt gttatccttg tcgtaggctg cctgggaggc atcaagtttt
    10381 ctatccataa aggtgaaaat aaagaatgaa atgaagccta tgcaaagcat gattaggcaa
    10441 accaaaatgg gttttgagac gtgtcctaga gattttgcta ttggaaccga tgtggctagt
    10501 gccagagcag ttcccattcg ggcagtagcc atctcgagcc ccatggcaag tgcaagttct
    10561 ttccccttaa accatttcac aattattttc gaaacggtta ttcctgccac ttcaacgcca
    10621 actccaaaaa ctgcatatcc cagcgcagcc ataaaaacct gagctttcat cggaaacaac
    10681 cagagtatat tccatacctg cccatcaagc gaatgggttg atacagccca aaacttaata
    10741 gcagttccgg taatcatcac cgctgctgct cccaggccag taacccttac ccccatttta
    10801 tccagaataa tgccaccgaa aatcagcatt agcaaaaaaa cattaaacca accgtaagca
    10861 ctggtaaata ttccatactc cgtgctgttc catgtaagct gctcttctag caagccctta
    10921 agcggagcca ttacgtcggt taggtagtaa ccggttagca ttgtaaaagc aacaagagcc
    10981 aaagccgtcc atcgtgctac tgcagagtca cgaagtgtct ctctaatttt ttccattctc
    11041 tataaattaa tttaattatt gatttttaag ggttgaaaaa aatttcaaac gaacgcaaat
    11101 aaacttaaat ccaatctaaa atcctatgag attgaccaat atttgaaaat aaaaaaccgt
    11161 catgaaaatg atgacggcta aagagtttag ggaatgaagc agctactgtt tttcgtaaac
    11221 ccacatttcc ataggattct ctatcctgtt ggagtttatc cggtcaattt cgttaaaagc
    11281 cttgctgtag gtattgaagg caccaaccga tactagatta aagccgtttg gcgcttggag
    11341 tattcgggtg ttgttaaacc ccatacccct gacttcctgc tcccagctag tggcgttgga
    11401 tggaaccttg aagctgccaa taataacatg aaaccggtgc tggttttcat attcttctac
    11461 ctgcctgatg gaatcctggt gtgcctgctc ccttgccaga gcggccttct caaattctat
    11521 tgcctttgcc ttggcaaggc tatcttcctt tgctttctgc atggcttcgg ctttagcttt
    11581 cttgcctttc ttggtatatt tttcaacaaa cttgcatccg cttcctagca gggtcagcaa
    11641 aattagcaca caggtaagtt ttttcatttg gagtttgggt tagttgtata tttcaatagt
    11701 tgtgcaagtt agcaaaagtt atgctaaaaa tgcaagtgca atccctttaa aatttccccg
    11761 tccttccaat tttattccta agggttccga ttcttcatgt taaaaaagtg tgttaacaca
    11821 gcggaagggt aaaccgatag cccggctgga tgggtttctt ccatttaaaa aattatagct
    11881 ttacctactc aaaagggtta ctccgtgaaa aatcgggttg gtagcttccg aaactctcga
    11941 ggtcctgaat ccagccccta aaaccgattc cctgagcaac ctctactacc cccatatatt
    12001 cctccattgt aaccgttctg tttagagggg actggagctc cactcccacg gggggatagt
    12061 actgcgacat cagcgagagg taaatgcgcg gcgtaaactc ttcggcaagg aatttgagta
    12121 gcctaatact ctcatcggca tagccgggaa gcaccaagtg gcgaataatc atacccgatt
    12181 cggccaaacc atcgttgtca accagcagcg agctcccctt ctgacggtac atctcgcgaa
    12241 tggcttttgc cgcaacatcc gggtagtctg ccactcccga gtagcgcaag ccaagttcag
    12301 ggttaacata cttaaaatcg gggaggtaaa tatccacaat tccctcaagc tgcctgaggg
    12361 tttcaacgct gtcatagccg tttgagttgt aaataattct gggagagtat ccttccctcc
    12421 tgactagctc cagaatctcc aacatctgaa ccacctgatg cgaaggcgaa acaaaaccca
    12481 acatgctaat gccacggtta agcagcccac aaatttcctc tgcaacctca tttacactca
    12541 tcttccactc ccgattcgta atggagttcc ggctaatctg atggttctgg cagtagctgc
    12601 actgaaggtt gcagtgtgca aaaaatacgt tgcaaactcc ttgctcaccc gaaagcaccg
    12661 gttcctctcc cctatgcacc acaaccgatg aaaccagagg agtatcgggg gttttacaaa
    12721 tacccctcac catactacga tttgccccac agccgtgcgg gcaaagatta caacttaaaa
    12781 gattaccatg atcaatcacc taaataaatt tttgcaaata tacgcacttc ccattttttc
    12841 ttgtctctca actcacctaa cctggttgct tacattatga ttgttttgtg ttcaacatac
    12901 aggctgttcc gctttgttgt tattacaata catttttgac ccaacaaccc attgtaataa
    12961 tttcaaggtt gaagccctca gggcatcctt ctcgttaatg cagctttaac caggctaatt
    13021 attgttggct agtatcaaac aattttatct atttgtaagc aagataaccc tttcgtgtat
    13081 ccatttgtaa cacgaacaat ataccccaaa aatactacgc cccttggtac attctctaaa
    13141 tggtgtacat ttgccctagg aatcactagt tttacctgcc tatctgtcag aaaaccagta
    13201 gttttagcac accaaaaaga aaaataaatg tagatgaaca gaattttgga tttaaactac
    13261 tatgataaag aaaaggatgt ataattttgt aacaagttaa atattaacac attaaaacat
    13321 agttcggatg aaaacaatca tcaatctttt tgaggaaagt gtaacgaaat accccgataa
    13381 cgcctgcatt tgggaaaagc taaccgataa gtatgaatca tcctcatatt ctgaggtaag
    13441 gaaggaggtt taccggctgg cagcaggttt tcttagccta ggaataggga aaaacgataa
    13501 ggtagcactg ctcagtgagg gtagaaagct gtggatcacc agcgaactgg cgattcttta
    13561 cactggtgca gtaaacgtcc ccctgtcggt taagctggat ggcaatgatc tgcttttccg
    13621 tttggttcac tccgaaacct caaccattat tgtttcagga atacaggcac ctaaaataac
    13681 aagaattaag aaggaaatac ccaacttaaa aaggataatc taccttgatt cgttgaatca
    13741 atactccgaa ggtgaaatgg gaatagagga ggtattcaag cttggcgaca cttttctagc
    13801 agaacatcct ggaagagtag aggaggtttc ctcatcagta aaaccttccg acttcgccaa
    13861 catcagctac acctccggaa ctactgccga ccccaaaggc attatcctat cccataataa
    13921 ctatacagca aatgtggagc aggccctaac cttaatggat attcctcaaa gctacagaac
    13981 actcctcata cttccgctcg accactgttt tgcacatgtt gcaggtgttt acgcctttat
    14041 ggcaatggga gctagcgctg caactgtgca ggtaggtaaa tcggcaatag ataccctcaa
    14101 aaatattccc atcaatatca aggagataca acctagctta ctgctaagcg tgccctcgct
    14161 agccaaaaat ttcaaaaaaa atattgaagt cggtattagg gaaaaaggga aatttaccga
    14221 aaagctgttc aacaatgccc ttaaagtctc ataccggtac aacaaagaag ggtggaataa
    14281 aggaaataat tttacattta tttacaagcc tttaatctgg atatacgaca agttgatatt
    14341 cagcaaagta agagaggttt tcgggggccg tttggaattt tttattggtg gtggagcgtt
    14401 actagacatg gagctccagc gctttttcta tgcaattggt attcccatga tgcagggata
    14461 cggcctttca gaagcaacgc ccattatcag ctccaactcc ctaaaaagac ataagcttgg
    14521 ttcatctgga tacctggtta aacccctcga tcttaaaata tgtgatgaat ccggcaacga
    14581 acttcctgtt ggccaaaagg gtgaaattgt tgtccgtggc gagaatgtca tggttggcta
    14641 ctggaaaaat gaaaaagcca ctgccgatac tatacgaaat ggttggctcc acacaggtga
    14701 catggggtac atggataaag atggtttcct ctacgttctt ggccgtttta aaagcctcct
    14761 gatttcgagc gatggagaaa aatacagccc cgagggcatt gaaggagcac ttgaagaaca
    14821 ctcaaagttt attgaaaatg ttatcctata taacaaccaa aatccttaca ctatagcgct
    14881 gatagtgcct tcaaaagaat ctattcggag agctctaaac gaaaaaggga ttgatatgaa
    14941 cactcacgaa gctggtattg aagctatcaa gctaatcaac aatgaagtgt cccgatttaa
    15001 aaccggaggc gaccttcaaa acttatttcc ggaacgctgg ttgccttcag cattttcgat
    15061 tcttgaagaa ccatttacag aagataatgg tttaattaac agcacaatga aggttgttcg
    15121 gggtaaggtt gaagaaaagt atagggataa aatagagcat ttatacactg tggaaggaaa
    15181 aaatatatat aacaactcaa atataagctc aatgcttaaa attataagca actagtgcta
    15241 tttatatatt ataacctgag ttagcttgat aaataaaatt agtcagttaa aatgaacata
    15301 cacgtataaa ataaaactag aagatattta aacaaccaat cctaataatc taaagacaca
    15361 tataagattt aaatccaact aggagaataa aaaaagaacg tatataccgg ggaaacaaat
    15421 caaggtgcta taataaaaaa aagccccggt cggggtaacc cggccggggc ggaggtgggg
    15481 cggcg
//
LOCUS       DAOSHM010000056        15448 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_3138_length_15448_cov_20.205613, whole genome shotgun
            sequence.
ACCESSION   DAOSHM010000056 DAOSHM010000000
VERSION     DAOSHM010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 15448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15448
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3138_length_15448_cov_20.205613"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            85..1197
                     /locus_tag="PK839_08440"
     CDS             85..1197
                     /locus_tag="PK839_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007903245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-deoxy-7-phosphoheptulonate
                     synthase/chorismate mutase type II"
                     /protein_id="HOG72801.1"
                     /translation="MDTPLNITPISSWFEGLSMPLIIAGPCSAESEEQVMDAALQLAG
                     IERVKVFRAGIWKPRTRPGSFEGAGEKGLRWLQRVQRETGLLVTTEVATPAHVQMALD
                     YGIDIVWIGARTTASPFTTEQLAVALSHSNIPVLVKNPITPDLELWIGALERFNRMGI
                     TKLAAVHRGFYPYEHSRLRNIPKWELAIDLKSRFPDLPILCDPSHIAGRADMVAQVAQ
                     HGLNLSLDGLMVEVHPNPSAALSDARQQVSPHVFSQLLQKLIFRDSSSACSEYVDFIE
                     QTRNKIDSIDQQIIELLANRMKLVEQIGEYKRDNNVTVFQLRRWERILESRVEYGERL
                     GLSEEYIKNLLRMVHKESIRRQAEILGSKSQPNSNE"
     gene            complement(1199..1456)
                     /gene="purS"
                     /locus_tag="PK839_08445"
     CDS             complement(1199..1456)
                     /gene="purS"
                     /locus_tag="PK839_08445"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF004630.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HOG72802.1"
                     /translation="MKFTAEINVMPLKALLDPQGKAVNSTLHGIGHTEVSNVRIGKHI
                     TMEVESASREEAIEKVTEICSKVLSNPVMEGYDFTLNEIKS"
     gene            complement(1560..2315)
                     /gene="pssA"
                     /locus_tag="PK839_08450"
     CDS             complement(1560..2315)
                     /gene="pssA"
                     /locus_tag="PK839_08450"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HOG72803.1"
                     /translation="MPTGKSITKQLPNVITLLNLVSGCLSIVAALDCDLQLAGLFIIL
                     AAVFDFLDGFTARLIGAYTMLGKELDSLSDVVSFGVAPSMIIYELMRQSLELPKNEGL
                     LNGHWLLLIPFVMAAFSSLRLGMFNLDERQSSSFIGLPTPANALMIVGLTFGLNSTWA
                     DVYGIFTSSVWALTAIVALQSFLLVCELPMFSFKIKSLKLSVAYKQLLFLSGAAVLVV
                     IFLKAALSLIIIWYVFLSLVFYLTGKMGKKQHV"
     gene            complement(2618..4708)
                     /locus_tag="PK839_08455"
     CDS             complement(2618..4708)
                     /locus_tag="PK839_08455"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007659276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HOG72804.1"
                     /translation="MIAIGAIVSCKSEKQTDNPFLEDYTTPFGVPPFDKIRVEHFEPA
                     IMEGIKQQLAEVEAITTNSEKPTFENTIIPLDYSGELLTKVVLTMENINSANTSEELQ
                     EMLKKVSPVLSKHRDDISMNPKLFERVKMVYSSMNESSYTPEQKKLIENTYKDFVRGG
                     ANLDAAKQEELRKINQELAMLALKFNENLLADNNAYKIVIENKDDLAGLPESVIAMGA
                     ADAKARGLEGKWVYTVDKPSMLPFLTYSQNRKLRDTLYYGYYMRGNNNNANDNKDIIK
                     KIVDLRQQKASLLGFPTHANYVLDVNMAKTPENVYDLLGKLWTPAIKKAKVERDDMQK
                     LANAEGANFKIGAADWWYYAEKVRKEKYDLDEEMLRPYLSLEDVRNGVFNLAGKLYGL
                     KFIKHTDIPVYHPDCEVYEVQEADGRHLGILYLDFFPRDSKSGGAWCTEYRSYKVKPD
                     GTISTPVVSIVTNFTKPAGDTPALLSFDEAETFFHEFGHALHSLFSKVQYPGLANVPQ
                     DFVELPSQIMEHWASHPKYLKEYALHYKTREPMPDKLISKIEASSQFNQGFATVEYLA
                     ASILDMDYHTLTAEKAKIEDVLAFEKASMDRIGLIPEILPRYRSTYFGHIFSDVLGYS
                     SGYYSYIWAEVLDSDAFEAFVESGDIFNKEVAGKFRTCVLEKGGSKEALELYKDFRGR
                     EASIEPLLKNRGLK"
     assembly_gap    4809..4908
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5014..5715)
                     /locus_tag="PK839_08460"
     CDS             complement(5014..5715)
                     /locus_tag="PK839_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72805.1"
                     /translation="MRFRSKLIWVTLFAIAMGLLESAVVIYMRELLYPGGFSFPLSPI
                     PERLAVTELLRELATLVMLVGVGVVAGRNFPERFAWFIFSFGVWDIFYYVFLKLLIGW
                     PTSMLTWDLLFLIPVTWTGPVVTPVVCSMLMITLALVILRRTGKNPTFRISPLSWVIL
                     IAGAMVVFLSFITDYTRFILHSFTLRELWSLPSKQALFDLSVQYIPFSFPWMIFAAGS
                     AVIIAAGIRIFNENR"
     gene            5857..7200
                     /locus_tag="PK839_08465"
     CDS             5857..7200
                     /locus_tag="PK839_08465"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HOG72806.1"
                     /translation="MGAFHAYDIRGIYNKDFNKDDAYKIGFFLVELLKTDRVLVGRDV
                     RMSSDEIFTYLTRGITDAGADVYNLGLATTPMVYYATAKGDFTASVQITASHNPKEYN
                     GLKVSGRNAEPIGYDTGLKFIEERMKSSSVVPVKDTGRVIDYDIRKDYITFLNSFKQD
                     YSNLKFAVDCSNGMAALLVKDILGNSPVYIYDELDGSFPNHEANPLIPENVVDLQNLV
                     RKQKCDLGIIFDGDADRVMFVDEHSHFISPDLMIAVLGHYFLEEKGFRGNVLQDIRTS
                     KAVGEYLAPMGATMHTWKVGRAFAAKKLRELDGIFGGELAGHYYFRDFFYSDSGMLAC
                     ILILNVVSKMKRKGISLSQLIGSIATYANSGEINFRIEKKQEAMDAVRNFFTATEKPV
                     AIYDFDGYRVEFSDWWFNIRPSNTEPYLRFIAEAKSTEMLDAKVSQVRRIIEEMK"
     gene            complement(7231..8589)
                     /locus_tag="PK839_08470"
     CDS             complement(7231..8589)
                     /locus_tag="PK839_08470"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOG72807.1"
                     /translation="MKRINIYSALTVSLLVVSFGCHKPKPYDLIVENAIIFETTEGKA
                     SPALKTILINADTIAGIVDYETPVRTKKVVDADGRLVTPGNIDTHVCLQNVIEGSKNP
                     QSSYPKVLTNYYRNNFSRRYLPYGITTALDLEPNDSWTSQILDWTPNPKFTTLLVAYQ
                     LPKVSFISSNRPTTPNIDSIAKALQSKGLGYVFLDEQEAVFPQKVYEMLKGKGMVVFT
                     RPHFGTFLRDSSKNIVSISTLISNALHYISKQNDLSRQIKNVYGSIDTFPKELFILEA
                     FKYMVENEPALLDKIAQEFGNRKATISSDLYGMAEFAGLTPERNLSLTLPRELQHRAE
                     YNFNHLLSYVKKLHDMGVKLLIGTNSPLGGLAYETEQMLLSEAGIPASDVLVISTLNA
                     AEALGISSKTGCIKVGSKADLIIYDMNPLEEPSNFTCTRRVINGGKLYKRNSQKTEES
                     EL"
     gene            complement(8894..10999)
                     /locus_tag="PK839_08475"
     CDS             complement(8894..10999)
                     /locus_tag="PK839_08475"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016277596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HOG72808.1"
                     /translation="MSKNNLLIASAMLLFATLTAQGQEPSNPFFSVYSTPFQVPPFNL
                     IAIEHYAPAFERGIEEQKKEIEAITSNPQAPNFENTILAFDKSGELLTKVSSVFYSLN
                     SANTSPEMQKVARVISPLVTAHRDNINLNDKLFQRIKAVYTKRDSLKLDSLQKRTVEK
                     YYNDFVRNGANLDEAAKARLREINQQLSELGLHFGENLLAETNKNFKLVIDNPADLDG
                     LPKDIVDAAAITAKEFGLEGKWVFTLQKPSMIPFLQYAKNRNLREKLYTGYAMRCNNN
                     DEFDNKSIMLQIVNLRAEKAKLLGYKTYADYSISNNMAKTPEAVYDFLNQVMEPALIA
                     ATRDRDDMQAIINREGGTFKLAPWDWWYYAEKLKKEKFNLDESELKPYFKLENVRDGM
                     FYVANQLYGITFTRRTDLPIYHPDVEVFEVKNETGAHIGLLYLDYFPRAGKRVGAWCG
                     RFRQQTYENGKRITPIVTMVTNFTPPTGNTPALLTWDEVTTLFHEFGHALHGLFTDGP
                     YDRIAGSLPRDMVELPSQVMENWAGEPRVIKVYAKHYQNGSPMPEDLINRLKKSMTFN
                     QGFETVEYIAASVLDLDWHSITEPQKVDVLQFENNSMKRIHLLDEIYPRYRTTYFNHI
                     IGGYAAGYYVYLWAAVLDTDAFNAFAETGDIFNHEVATRFRKHVLTEGGNDEGMIQYV
                     KFRGKEPSVEPLLKKRGFK"
     gene            complement(11099..13642)
                     /locus_tag="PK839_08480"
     CDS             complement(11099..13642)
                     /locus_tag="PK839_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HOG72809.1"
                     /translation="MRTLSKYKIIPENAMKRNLLTVSLMVLVNTIIFGQNIPIEKANY
                     ELPARFSPKKVEKMVFSTTVQPHWLKNSTRFWYSYETPSGKSYIIVDPAKRSKGFLFD
                     NNNMAAQLSLLTKEPFDAQHLPIQKIKFIKNETAIQFEVKSTLVDEDKSDEDEENDMV
                     KKKEGKAKAKPDKKTYYFEYDLATRKLMLLEDYKKPKENPEWANISPNGEMVVFSRHH
                     NLYWMDKENYEKAKKNEDDSTIIEHQLTTDGEKYYSYGYTRENEDNVDEEKNKDKRKP
                     TYITWSFDSKLFAMTRTDMRNVKELWLVKTLSSPRPTLETYKYHMPGEKEAPKDELLI
                     FNLAQDTIIKPDVAAFKDQDLMILNARVKANERDDDYVATRWLSKYNDRLYFVRTSRD
                     LKRVDVCYVDTKTGKVNVVIEERLNTYIDFNGISLINNEKEIIHWSERDGWGHFYLYD
                     NNGKFIRQITSGPFHCENLVGYDETTKTLFFTANGCEPNEDPYYLHLYRVGLDGNGMK
                     LINPGNFDHYISMDDGNRYFVDNYSRVNTNPETRLLDAQGNELLALEKADLSLLFQAG
                     YKFPEIFKVKAADGITDLFGVMYKPFDFDSTKLYPLIEYVYPGPQTESVNKSFSARLD
                     RTDRLAQFGFIVITVGNRGGHPARSKWYHNYGYGNLRNYGLADKKAAAEQLAYRHNYI
                     DINRVGIHGHSGGGFMTAAALLTYPDFFKVGVSNAGNHENNIYNRWWSEKHHGVKEIT
                     DTSGKVSFEYSIDKNSEIAKNLKGHLLLTTGETDNNVHPGNTIRLANALIKANKRFDL
                     FMFPGQRHGYGDMTEYYFWLMGDYFSKYLIGDYSTSVDITPMNNEMEKR"
     gene            13855..>15448
                     /locus_tag="PK839_08485"
     CDS             13855..>15448
                     /locus_tag="PK839_08485"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HOG72810.1"
                     /translation="MKRLLCIFILAALPAVAYLQPPLLTVAESSNFTSTSTHSEVMAY
                     VERLQKTSKFLRVEKIATTTEGKDLPLLVIGNPVPKDPKDLHGRLVVYIQANIHAGEV
                     EGKEASLMFARDLVAKRDPELLKHLVVLICPDLNADGNDRISKLNRTYQNGPANGVGE
                     RQNGQWLDLNRDAMKLESPEMRGVVANVLNRWDPAIIVDCHTTNGSYHQEPVTFTWMM
                     NPNGDRELIRYMRDRMMPWVSQTLSGKYGTLNCFYGEFINQQNYEEGWISYASEPRYF
                     SNYVGIRNRLGILNENYVYAGYRERVLGCYNLIASITDYALAYQTEIMQMLARADSIS
                     LAIGSQAASSDSFAVKYSGTPTPQKITILTYEAEAYTDEHGRERFRKTDTKRTVTVPY
                     IADYIPVKSIAYPYAYILPVPDPNVLQLLRQHGVEFQMLTDSVTLTVEQFAIDSIAPD
                     RRLNQGHYCNTINGSYQKVSRRFNKGDMVVKTSQKLGRLAAYLLEPQSDDGLVYWNFW
                     DRYLVPQWGSYFFPVPVYRLTSP"
BASE COUNT         3828 a         3442 c         3702 g         4376 t
ORIGIN      
        1 ctgttttagc agctggttgt tagttttacc cttaccagtt gattatttct cactaacttt
       61 gtcaaatatt tattgggtta aagtttggat acaccactta acataacacc aatttcgagt
      121 tggtttgagg ggttatcgat gccgttaatt attgccggac cctgtagtgc cgaaagcgag
      181 gagcaggtta tggatgcagc ccttcagctt gccggaattg aaagggtaaa ggttttcagg
      241 gcaggtatat ggaagcctcg cacacgccct ggcagctttg agggtgccgg cgaaaagggt
      301 ttacgatggc ttcagagggt tcaacgcgaa acgggcttac tggtaactac tgaggtggcc
      361 actcctgctc atgttcagat ggccctcgat tatggaattg acatagtttg gattggcgcc
      421 cgaacaacag ccagcccgtt cacaactgag cagcttgctg tagcccttag ccatagtaac
      481 attccggtgc tggtgaaaaa tcccattacg ccggacctgg agctgtggat aggtgcgctt
      541 gaacgcttca accgcatggg aattaccaag ctggctgccg tgcatcgcgg tttttatccc
      601 tacgagcatt cacgcctacg gaacattcct aaatgggagc tggccataga tttgaaaagc
      661 cgattccccg atttgcctat tttatgcgat cccagccata ttgcgggtcg tgccgatatg
      721 gttgcacagg tggcccagca tggcctgaat ctttcgcttg atgggcttat ggtggaggtt
      781 catcccaatc catcggctgc ccttagcgat gcccgtcagc aggtaagtcc ccatgttttt
      841 tcgcaactac tgcaaaagtt gattttcagg gattcctcgt cggcatgctc tgagtatgtt
      901 gacttcattg agcaaacccg taataaaatt gactccattg atcagcagat tatcgagctg
      961 cttgcaaaca ggatgaagct ggtggagcaa attggtgagt acaagcgtga taacaacgtt
     1021 acggtttttc agctgcgtcg gtgggaacgc atactcgaat cgagggtgga gtatggcgaa
     1081 aggttagggt tgagcgaaga gtacatcaaa aacctcctcc gcatggtcca taaggagtcc
     1141 atcagaaggc aagctgaaat tttaggttca aaatctcagc ccaacagcaa cgagtaggct
     1201 agctcttaat ctcgtttagg gtgaaatcgt aaccctccat caccgggttc gagagcacct
     1261 ttgagcaaat ctccgttacc ttttcaatgg cctcctcccg gctagccgac tccacctcca
     1321 tggtaatatg cttacctatt ctaacattcg acacctcggt atgcccaata ccatgaagag
     1381 ttgagttaac agcttttccc tgtgggtcga gtagcgcctt tagcggcata acgttgattt
     1441 cggcagtaaa tttcatgcgt ttgaacttat ttagccggct atacaataaa atttagcagg
     1501 actacttgat ttatgatgac aaaaataggg gatttttaga atcaccggtt gaatttagct
     1561 caaacatgtt gctttttccc cattttcccg gtaaggtaga aaaccagcga tagaaatacg
     1621 taccaaatga taatgagcga aagtgcggcc ttcagaaata tcacaaccag aacggcagcg
     1681 ccggaaagaa aaagtagctg cttgtaggca acgctcagct taagcgattt tatcttgaac
     1741 gagaacatgg gcaactcgca aaccaagaga aacgactgga gggctacaat ggcggttaat
     1801 gcccaaaccg aacttgtgaa aattccgtac acatcagccc atgtgctgtt gaggccaaag
     1861 gtaaggccta ctatcatcag cgcattggca ggggtgggta acccaatgaa tgatgatgac
     1921 tgccgctcat caaggttaaa cattcccaat cgtaacgacg agaaggcagc cattacaaat
     1981 gggattagga gaagccaatg gccattcaga agaccttcgt tttttggcag ctccaggctt
     2041 tgccgcatca gctcgtagat gatcatagag ggtgcaacgc caaagctaac cacgtccgag
     2101 agcgagtcca gctccttccc cagcatggtg tacgcaccta tcagcctggc cgtaaaccca
     2161 tcgagaaagt cgaacaccgc tgccaaaatg ataaacagcc ctgccagctg gagatcacaa
     2221 tcaagcgctg ccactatcga aaggcatccc gaaaccaggt taagcagggt gattacgttg
     2281 ggcaactgtt tagtaatact tttacctgtt ggcatggcgc tataatttgg ggctaaaatt
     2341 agagtacaaa ttcgtcaagt tcaaaaataa aattctaaat gaatgaaaat tcatgggttt
     2401 gttaaatggt aaaaaggcaa aactgttgca gaaagcattt cagaaatttt tcattaggcg
     2461 ggatgccgat gaatgctcat tacgttgact tgaatatgtc gggattaagc taacaccgga
     2521 gattgatgag ctttaccagg cagcaaccgt tagtatgact tgtaactaaa aaaggggctc
     2581 ggtttgagcc ccttttggtg ttatgtgtaa tggcgtgcta tttcaatcct ctgttcttta
     2641 gaagcggttc aatgctggct tcacgacctc gaaaatcttt gtatagctcc agggcctcct
     2701 tggaaccacc cttttcgaga acacaggtgc ggaattttcc tgctacctcc ttgttgaata
     2761 tgtcgccgct ctccacgaag gcttcaaagg catcgctatc gagcacttcg gcccaaatgt
     2821 aggagtagta tccgcttgag tagcccagca catcgctgaa aatatgccca aagtaagtgc
     2881 tgcggtagcg gggtagaatt tcgggtatta acccaattct atccatcgag gctttttcaa
     2941 aggccagcac atcttcaatt tttgcctttt cagcggtaag ggtgtggtag tccatatcaa
     3001 gaatggatgc agccagatac tcaaccgttg caaagccctg gttgaattgg ctgctggctt
     3061 caatttttga aatcagctta tcgggcatgg gttcccttgt cttgtagtga agggcgtact
     3121 ccttcaggta tttcggatgg ctggcccaat gctccatgat ttggctaggt agctcaacga
     3181 aatcctgcgg aacattagcc agtccggggt attgcacttt agagaacagg ctgtgcaggg
     3241 catgaccaaa ttcatgaaag aaagtttcgg cttcgtcgaa actcaacagg gcaggagtat
     3301 cacctgctgg cttggtaaag ttggttacaa tggacaccac gggggtagaa atggtgccat
     3361 cgggtttgac cttataggaa cggtactcgg tgcaccaggc cccacccgac ttgctatccc
     3421 tggggaagaa atcgaggtag aggattccta aatgccggcc atcggcctcc tgcacctcat
     3481 aaacctcgca gtcgggatgg taaacgggga tatcggtgtg ttttatgaac ttcaacccgt
     3541 aaagtttccc tgctaggttg aaaacgccat tcctcacgtc ctcgagcgac aggtaggggc
     3601 gaagcatctc ctcatcgagg tcgtattttt ccttacgaac cttttcggcg tagtaccacc
     3661 agtcggctgc accaatcttg aagtttgctc cctcagcgtt ggccagcttc tgcatgtcgt
     3721 cgcgttcaac ttttgctttt tttattgccg gagtccagag tttacctagt aaatcgtaaa
     3781 cattttcggg cgtttttgcc atattcacat cgagaacata gttggcgtgg gttggaaagc
     3841 ccaagagtga ggccttttgt tgccttaggt caacaatttt tttaattata tccttgttat
     3901 cgttggcatt gttgttgttg ccacgcatgt aatagccgta gtaaagggtg tcgcgtagct
     3961 tgcgattttg ggagtaggtg aggaatggaa gcatgcttgg cttatcgacg gtgtacaccc
     4021 atttcccctc caaacctctt gcctttgcat cggcagcacc catagcaata accgattcgg
     4081 gaagacctgc aagatcgtcc ttattttcaa taacaatttt gtatgcgttg ttgtcggcaa
     4141 gaaggttttc attgaacttt agggcaagca tggcaagctc ctggtttatt tttctgagct
     4201 cttcctgctt ggcagcatca aggttggcgc cacctcttac gaagtcctta taggtgtttt
     4261 caataagttt tttttgctcg ggggtgtagc tgctttcgtt catactgctg taaaccatct
     4321 tcacccgttc aaaaagttta gggttcatgc tgatatcgtc gcggtgtttc gatagaaccg
     4381 gagaaacttt tttaagcatc tcctgcagct cttcgctggt gttggccgag ttgatgtttt
     4441 ccattgtcag cacaaccttt gtcagcaact caccgctata gtccaacgga attatggtat
     4501 tctcaaatgt gggtttttcg ctgttagtag taatagcttc aacttcagct agctgttgct
     4561 taataccctc catgattgcc ggttcgaagt gctcaaccct tatcttatca aatggtggaa
     4621 cgccgaatgg cgtggtgtaa tcctccagga atgggttgtc tgtttgcttt tcgcttttac
     4681 atgagacaat agcgcctatg gctatcattg tcattaaaat ccagctgtat cgtttcattt
     4741 gattgtatag tttaatttaa gttaacacga atagctattt acttgtctgc aagcaaagat
     4801 aatggatann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnat atttaaaaaa
     4921 atgaaagatt gatggtgaat gataaggaaa actaaacctg cacggctttt gccggaagcc
     4981 tgtacggcat tcacgttttt ttagctatta acgctatcgg ttttcgttaa aaattctaat
     5041 acctgcggct attattacag cactgcctgc agcaaatatc atccatggga atgaaaaggg
     5101 aatgtactgt acgctcaaat cgaacagagc ttgttttgat ggcaacgacc atagctcccg
     5161 taaagtgaag ctatgcagaa taaacctagt gtaatcggta ataaatgata ggaatacaac
     5221 catggctccg gcaattagaa ttacccatga gagtggtgaa attctgaatg ttgggttttt
     5281 ccccgttcgc ctaaggatga ccaatgcaag ggttatcatc agcatggagc atactacggg
     5341 cgtaactacg gggccagtcc acgtaaccgg aatgaggaat agcaaatccc atgttagcat
     5401 cgaggtgggc cagcctataa gcagctttaa gaaaacgtag taaaatatat cccaaacgcc
     5461 aaagctgaaa ataaaccatg caaaacgctc agggaagttc ctcccggcca ctacgccaac
     5521 tccaacaagc ataaccaggg ttgccaactc ccgaagcagc tcggtaactg ccaacctctc
     5581 gggaattggg gagagaggaa aggaaaaacc accagggtat aggagctcgc gcatgtaaat
     5641 tacaacggcg ctctccagta gccccatggc tatggcaaaa agcgtaaccc atatcaattt
     5701 acttcggaat cgcataattt tctgtttaac ctatcaaatt tactcttttt tactgacttt
     5761 ggtcaatatt atggccaaaa ggtttcaaca aggaataatg tttttaattt tgccagccaa
     5821 agtaaattga tgataactta accggtaaac aggactatgg gtgcttttca tgcctacgat
     5881 attcgtggaa tttataacaa ggattttaac aaggatgatg cctataaaat tggctttttc
     5941 ctcgttgaac ttttgaaaac cgacagggtg ctggtaggac gtgatgttcg catgtcgtcc
     6001 gatgaaattt tcacctacct gactcgtggt ataaccgatg ctggtgcgga tgtttataac
     6061 ctgggattag ccaccacgcc aatggtttac tacgcaaccg ccaagggaga tttcacggca
     6121 tcggtacaga taaccgcttc gcataacccc aaggagtaca acgggttgaa ggtatcgggc
     6181 aggaatgccg agcctattgg ctacgacacc ggtttaaaat tcattgagga gaggatgaaa
     6241 agctccagtg tggtacccgt gaaggacacc ggaagggtaa tagattatga tataaggaag
     6301 gattacatca ccttcctcaa cagcttcaag caagactatt caaacctgaa gtttgcggtt
     6361 gactgctcca acggaatggc tgcacttttg gtaaaggaca tactgggcaa tagcccggtg
     6421 tacatctacg atgagctgga cggttcattc cccaaccacg aggctaaccc ccttataccc
     6481 gagaatgtgg ttgacctgca aaacctggtg agaaagcaga aatgcgacct tgggattatt
     6541 ttcgatggcg atgccgatcg ggtgatgttt gttgatgagc actcgcactt tatttcaccc
     6601 gacctgatga ttgccgtgct tggccactac tttctggagg aaaaaggatt tcgggggaac
     6661 gtcctgcagg atatcaggac atcaaaagcg gttggcgagt atctcgcccc aatgggtgct
     6721 accatgcaca catggaaggt gggaagagct tttgctgcaa agaagctacg cgagcttgac
     6781 gggatttttg gcggcgagct tgccggacat tactacttcc gcgacttctt ttactccgac
     6841 tctggaatgc tggcttgtat cctcatttta aacgttgtaa gtaagatgaa aaggaaaggg
     6901 atcagccttt cccaactaat tggtagcatt gccacctacg ccaactctgg cgaaattaac
     6961 ttcaggattg aaaagaagca ggaggcgatg gatgccgtgc gcaacttttt tactgctacc
     7021 gaaaagcccg ttgccattta cgatttcgat gggtacaggg tggagttcag cgactggtgg
     7081 tttaacattc gcccttcaaa taccgagccc tacctacgtt tcattgccga ggctaaaagc
     7141 accgaaatgc ttgatgccaa ggtaagccag gtgagaagaa ttatagagga gatgaagtag
     7201 ccctttgaat tttgaagagg gatcaggatt ttacaattcc gattcttcag ttttttggct
     7261 gtttcgcttg tatagcttgc caccattaat tacccttcta gtacaggtaa agtttgaagg
     7321 ttcttccagg gggttcatat cgtaaataat taagtcggcc ttacttccca cctttataca
     7381 accagtttta ctgctaattc ctaatgcttc agcagcgtta agggttgata ttactagtac
     7441 atctgaggca gggattccag cctcagacag cagcatctgt tctgtttcat aggccagccc
     7501 tcctaacggg ctattagttc cgatgaggag ttttacgccc atatcgtgaa gtttttttac
     7561 atagctgagc agatggttga agttgtactc cgctctgtgc tgcagctcgc gtggaagggt
     7621 taaggataaa ttcctttcgg gtgtgagtcc ggcaaattcg gccattccat acaaatcaga
     7681 tgatatggta gccttcctgt taccaaactc ttgggcaatc ttatcaagca gcgcgggctc
     7741 attctctacc atatacttga atgcttccag aatgaacaac tcctttggga aggtatcgat
     7801 gctgccatag acatttttga tttgacgcga aagatcgttt tgtttagaaa tgtagtgcag
     7861 tgcgttgctt attagtgttg aaatgctgac tatatttttg ctggagtccc ttaaaaaagt
     7921 tccaaaatgg ggacgagtaa agacaaccat tccttttcct ttcagcattt cgtaaacttt
     7981 ttgtggaaaa acagcttcct gttcatccag aaaaacatat cctagcccct ttgattgcag
     8041 agccttagca atgctgtcta tattgggagt ggtaggtcta ttggagctta taaatgaaac
     8101 ttttggaagc tggtaggcta caagcagggt tgtaaattta ggattgggtg tccaatccag
     8161 tatttggctg gtccagctgt cattgggctc taagtctaaa gctgtggtaa ttccgtaggg
     8221 caaatacctc ctggagaagt tgttgcggta gtagttggtt aaaactttcg gatagctgct
     8281 ttgcgggttt ttgctaccct caatcacatt ttgaaggcaa acatgggtat caatattacc
     8341 gggtgttaca agccgaccgt cggcatccac tacctttttg gttcttacag gtgtttcgta
     8401 atcaactatt ccggcaattg tgtcggcatt tatcaggatg gttttaagtg ctggagatgc
     8461 tttgccctcg gtagtttcaa aaattatggc attttcaaca atcaggtcgt agggttttgg
     8521 cttatggcag ccaaaggaaa ctaccagcaa tgacaccgtt aacgcggagt atatgtttat
     8581 ccttttcatt cgggttggtg ttgaaaattc aaaggtacta ctttttagga ttttttgggt
     8641 agtcttaccg ataagtttgg gtagagtctg atgatgggtg gaggttttct atgggttggt
     8701 aatatctgtt tataagcttg ttaccgttac cttcttatct aaatcggcaa ttgtcgggct
     8761 gtgaatggaa tttttgacca aaagtgcatg gtgggcagct gagctggatt tgaaaaaaaa
     8821 atgggctgca gcatttagct acagcccatt tgacccaaac aaacccttaa atatgtaaaa
     8881 gcactctgaa agactattta aatcctcttt ttttaagaag gggttcaacc gagggttctt
     8941 tccctctaaa cttaacatac tgaatcattc cctcatcgtt tcccccttca gttaatacgt
     9001 gtttgcggaa tctagtggcc acttcatggt taaaaatatc accggtttcg gcaaaggcgt
     9061 taaaggcgtc ggtatcaaga acagccgccc aaaggtaaac gtagtagccg gctgcatacc
     9121 cacctatgat gtggttgaag taggtggtgc ggtagcgtgg gtatatctca tctagcagat
     9181 gaattcgctt catggagttg ttttcaaatt gcagcacatc aactttctga ggttcggtta
     9241 tgctgtgcca atccaagtca agcaccgatg cagcaatata ctccacggtt tcaaaaccct
     9301 ggttaaaggt catgctcttt ttcaacctgt tgatgaggtc ttcgggcata gggctgccat
     9361 tttggtaatg cttggcgtaa accttaataa cgcggggctc tccggcccag ttctccatca
     9421 cttgtgaggg cagctctacc atgtcgcggg gtaggcttcc ggcaatgcgg tcgtatgggc
     9481 catcggtaaa taagccgtga agggcatggc caaactcgtg gaacagggta gttacctcat
     9541 cccatgtgag gagggctggg gtgtttccag tgggaggggt aaaattagta accatggtta
     9601 caattggggt aattcgtttt ccgttttcgt aggtttgctg tcggaaacgg ccacaccatg
     9661 cccctactct tttgccggct cgcgggaagt agtcgaggta gagcagccca atatgtgccc
     9721 cggtttcgtt ctttacttca aaaacctcca cgtcgggatg gtagatggga aggtctgttc
     9781 ggcgggtaaa tgttattcca tagagctgat tggccacgta gaacatgccg tcgcgcacgt
     9841 tttcaagctt aaagtagggc tttagctccg attcgtcaag gttgaacttt tcttttttca
     9901 gcttttcggc atagtaccac cagtcccaag gagccagttt aaatgtaccg ccctcccggt
     9961 tgatgatggc ctgcatgtca tccctatcgc gggtggccgc tatcagtgca ggttccatca
    10021 cctggtttaa aaaatcataa acagcttcag gtgttttggc catgttgttg ctgatactgt
    10081 agtcggcata agttttgtat cccaacagtt tggccttttc agcccgcaga tttacgattt
    10141 gaagcataat gctcttatta tcaaactcat cgttattgtt gcacctcatg gcgtacccgg
    10201 tgtagagttt ttccctaagg tttcgatttt tggcgtactg caggaagggt atcatgctgg
    10261 gtttctgaag ggtgaatacc catttaccct ctaatccaaa ttccttggcg gttatggctg
    10321 ccgcatcaac aatgtccttt ggtaggccat cgagatcggc aggattgtcg attaccagct
    10381 taaaattttt attggtttcg gccagcaggt tttcgccaaa gtggagtccc agttctgaaa
    10441 gctgttggtt tatctccctg agccttgcct ttgctgcctc atcgaggttg gcaccgttgc
    10501 gaacaaaatc gttgtagtat ttttcaaccg ttcttttttg gagcgaatcc agctttaggc
    10561 tatccctctt cgtgtaaacc gcctttatcc gttggaataa cttatcgtta aggttgatat
    10621 tgtcgcgatg ggctgttacc agaggtgaaa ttactcttgc caccttttgc atttcgggtg
    10681 atgtgttggc gctgttaagg ctgtagaaaa cgctgcttac cttggttagc agttctcccg
    10741 acttatcaaa tgcaagaatg gtattctcaa agttgggagc ttggggattg ctggttatgg
    10801 cttcaatctc ctttttctgc tcttcaattc ccctttcaaa tgccggggca tagtgctcaa
    10861 ttgcaatcag gttaaacggt ggaacctgaa atggtgtgga gtacacgctg aaaaacgggt
    10921 ttgacggctc ttgcccctgt gcggttaggg ttgcgaatag cagcatggcc gaggcgatta
    10981 gtaggttgtt tttgctcatt tctgttaaaa ttaaaattat aatttttttt actaggatgt
    11041 tttccttttg tcagtagttt aaacatgaaa aggaatggct aaaccttaag gcttggcttt
    11101 accgtttttc catctcgttg ttcatgggcg ttatatccac gctggttgag tagtctccaa
    11161 tgaggtactt gctgaagtag tcgcccatta gccagaagta gtactcggtc atatcgccgt
    11221 agccatgcct ttggccggga aacatgaata agtcgaaacg cttgtttgcc ttaatcagcg
    11281 cattggccag cctgatggtg ttacccggat gcacgttgtt gtcagtttct ccggtggtaa
    11341 gcagcaggtg tcccttcagg ttcttcgcaa tttccgaatt cttgtcaatg ctgtactcaa
    11401 atgacacttt tccgcttgta tcggttattt ccttaacccc gtggtgtttc tcgctccacc
    11461 agcggttata aatgttgttt tcgtggttgc ccgcattgga caccccaacc ttgaagaaat
    11521 cggggtaggt aaggagggca gcggcggtca tgaagcctcc tcccgaatgg ccatgtattc
    11581 ccacccggtt aatatcaatg tagttgtggc ggtaggctag ctgctcggca gcagcctttt
    11641 tatcggcaag cccgtagtta cgaaggttgc catagccata gttgtggtac cacttggagc
    11701 gtgcaggatg gccgcccctg ttaccaaccg taatcactat aaacccaaac tgcgccaacc
    11761 tgtcggttct gtctaggcga gcagaaaacg atttgttaac cgattcggtt tgcggtcccg
    11821 ggtaaacata ctcaatcagc gggtagagct tggtggagtc aaaatcaaat ggtttgtaca
    11881 tcaccccgaa caggtcggta atgccatcgg cagcctttac cttgaatatt tcggggaatt
    11941 tgtacccagc ctggaagagt aagcttaaat cggctttttc cagtgcaagc agctcgttgc
    12001 cctgggcatc gagtagcctg gtttcgggat tggtgttgac ccgggagtag ttgtcaacaa
    12061 aatagcgatt gccatcatcc atgctgatgt agtggtcaaa atttcctggg ttgatgagct
    12121 tcattccgtt cccgtcaagc ccaactctgt acaggtgcag gtagtagggg tcttcgttgg
    12181 gttcgcaacc gttggctgtg aaaaacaacg ttttggtggt ttcatcgtaa cccaccaaat
    12241 tctcgcagtg gaagggtccg gaggtaatct gtcggatgaa tttgccgttg ttgtcgtaga
    12301 ggtagaagtg cccccatccg tcgcgctccg accagtgaat aatttccttc tcgttgttaa
    12361 tcaggcttat accattaaaa tctatatagg tgttgagcct ctcctcgatt acaacattta
    12421 ccttgccggt tttggtgtca acgtagcaaa cgtcaacgcg ctttaggtcc cttgatgtgc
    12481 gaacaaagta gagcctatca ttatacttcg acagccacct ggtggcaaca tagtcgtcgt
    12541 cgcgttcgtt agcctttacc cgggcattca gaatcattaa atcctgatcc ttaaatgcgg
    12601 caacatcggg tttgatgatg gtgtcctgag ccaaattgaa tatcaacagc tcatcctttg
    12661 gtgcttcttt ctcacctggc atgtggtact tgtaggtttc cagcgtgggc cgtggcgacg
    12721 atagcgtttt aaccagccat agctccttta catttcgcat gtcggttctg gtcatggcaa
    12781 acagcttgga atcgaacgac catgtaatgt aggtgggctt gcgcttatcc ttatttttct
    12841 cctcgtccac gttgtcctcg ttttccctgg tatagccgta gctgtagtac ttttcgccat
    12901 cggtggtaag ctggtgctct ataatggtgg agtcgtcctc gttctttttt gccttctcat
    12961 agttctcctt atccatccag tataggttgt gatgtctgct gaacaccacc atctcaccgt
    13021 tgggcgaaat attagcccac tccggattct ccttgggttt tttgtagtcc tctaatagca
    13081 ttagctttcg ggtagccagg tcatactcaa agtagtatgt cttcttatcg ggctttgctt
    13141 tggctttacc ctcctttttc ttaaccatgt cattctcctc atcctcatcc gatttgtcct
    13201 catccaccag cgtgcttttc acctcaaact ggatagctgt ttcattcttg ataaacttaa
    13261 ttttctgtat tggaagatgt tgggcatcaa atggttcctt agtcagcagc gagagctgtg
    13321 cggccatgtt gttgttatca aagagaaaac ccttgctacg ttttgccggg tcaacaataa
    13381 tgtacgattt tccgcttggc gtttcgtagc tgtaccaaaa gcgtgtgctg ttcttcagcc
    13441 agtgtggctg aacagttgtg ctgaaaacca ttttctctac tttttttggc gagaagcggg
    13501 cgggtagctc gtagttggcc ttttctattg ggatattttg cccaaaaatt atggtgttga
    13561 caagcaccat caatgatacg gtaagcaggt ttcttttcat ggcattttcc ggaataattt
    13621 tatattttga taaagttctc attacatggt ttagctgtta aaccattaaa aaggcaaacg
    13681 gtcaaaagcc gtgttcctgt gtttaaaaac cgggtaacca aagggatgca gtttcaaaac
    13741 atgtaaactc acctgctaca tttttaaatg cttggatgga atgattttta aatatctcaa
    13801 aattcattaa agtttatttt ttctggacat agctaacaat aatattagga actgatgaaa
    13861 aggttacttt gcatttttat actagccgct ctgccagctg tggcttacct acaaccaccg
    13921 ctactaactg ttgccgagag cagcaacttt acctcaactt ctacccacag cgaggtgatg
    13981 gcatatgttg aaaggctgca gaaaacgtcc aaatttttac gggtggaaaa aattgccaca
    14041 acaaccgagg gtaaggacct gccgctgcta gtaattggaa accctgtgcc gaaagatcct
    14101 aaggatttgc atggccgttt ggtggtgtac attcaggcca acattcacgc tggagaggtt
    14161 gaaggcaagg aagcctcgct gatgtttgcc cgcgacctgg ttgccaagcg cgacccggag
    14221 ctgctgaaac atttagtggt gctaatttgt ccggatttga atgccgatgg taacgaccga
    14281 ataagcaagt tgaacagaac gtaccagaat gggcctgcta acggggtagg cgagaggcag
    14341 aacggtcaat ggctcgacct gaatcgtgat gctatgaagc tggaatcgcc cgaaatgagg
    14401 ggagttgtag ccaacgtgct taaccgctgg gatccggcta tcattgtgga ctgccacacc
    14461 accaatggct cctatcacca ggaacctgtt acttttacat ggatgatgaa tcccaacggc
    14521 gatagggagc ttattcgcta catgcgcgat aggatgatgc cctgggtatc gcaaacccta
    14581 agtggcaagt acggcacgct gaactgcttc tacggtgagt tcattaacca gcaaaactac
    14641 gaggaaggtt ggatttctta tgcctctgaa ccccgctact tctccaacta cgttggaata
    14701 aggaatcgcc tgggaatact taacgagaat tatgtttatg ccggttatcg ggagcgcgtg
    14761 ctggggtgct acaacctgat tgcctccata accgattatg ctctggcata ccaaacggaa
    14821 atcatgcaaa tgctggcaag ggccgacagc atatcgcttg ctattggctc acaagcggcc
    14881 agcagcgatt cgtttgcagt gaagtactcg ggcaccccca cgccccagaa gatcaccatt
    14941 ctcacctacg aggcagaggc atacaccgat gagcatggca gggaacgatt ccgaaaaacc
    15001 gacaccaagc gcacggttac cgtgccctac attgccgatt acattccggt taaaagtatt
    15061 gcctacccct acgcctacat cctgcctgtg ccggacccga atgttcttca gcttctccgg
    15121 cagcatggtg ttgaatttca aatgcttacc gatagtgtta cgttgacggt tgagcaattt
    15181 gctattgact ccattgctcc cgacaggcgg ttgaatcagg ggcattactg caacaccatc
    15241 aatggcagct atcaaaaggt gagccgccgt tttaacaagg gcgatatggt ggtaaaaact
    15301 tcgcaaaagc taggtcggtt ggctgcctac ctgctcgaac cccagtcgga cgacggattg
    15361 gtttactgga atttttggga caggtacctg gttccccagt ggggtagcta ctttttcccg
    15421 gtgccggtgt accgcttaac cagccctt
//
LOCUS       DAOSHM010000057        15333 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_3166_length_15333_cov_20.587511, whole genome shotgun
            sequence.
ACCESSION   DAOSHM010000057 DAOSHM010000000
VERSION     DAOSHM010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 15333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15333
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3166_length_15333_cov_20.587511"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(114..902)
                     /gene="dapF"
                     /locus_tag="PK839_08490"
     CDS             complement(114..902)
                     /gene="dapF"
                     /locus_tag="PK839_08490"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HOG72811.1"
                     /translation="MLITFSKYHGTGNDFVMVDNRSGGLNPTVDLIEKLCNRHFGIGA
                     DGLILLENAKESALMMRYFNSDGRESTMCGNGGRCFAHFAHQLGLAGQHVLFNGIDGF
                     HEAYLLDNGNIRLRMIDVPVVESAEEGFFLNTGSPHFVVFVRSVENVNVDLEGRLASK
                     SVNVKNGGTNVNFIQPVGEKVLKIRTYERGVEAETLSCGTGSVASAIAYADFFAISEG
                     PVELLAPGGTLRVYFSHEGDGYKNIWLEGAVSHVFNGSIEVNDL"
     gene            complement(1116..1976)
                     /locus_tag="PK839_08495"
     CDS             complement(1116..1976)
                     /locus_tag="PK839_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008625412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HOG72812.1"
                     /translation="MRQLKITKSITNRESASLDKYLQEIGKEDLITVEEEVELAQRIR
                     KGDQAALEKLTRANLRFVVSVAKQYQNQGLSLPDLINEGNLGLIKAAEKFDETRGFKF
                     ISYAVWWIRQSILQALAEQSRIVRLPLNQVGSLNKINKAFSKFEQEYERTPSPEELAE
                     LLDISKDKINDTLRISGRHVSVDAPFVDGEDNSLLDVIVNNDSPNADKGLINESLSKE
                     IERALATLTERERDIVRYFFGISCQEMTLEEIGEKFNLTRERVRQIKEKAIRRLRHTS
                     RSKLLKSYLG"
     gene            2393..3388
                     /gene="trxB"
                     /locus_tag="PK839_08500"
     CDS             2393..3388
                     /gene="trxB"
                     /locus_tag="PK839_08500"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008621000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HOG72813.1"
                     /translation="MNLFTPLNSESGKNLSGSPKPVEHVRVLILGSGPAGYTAAIYAA
                     RANLSPVVYEGIEPGGQLTTTTEVENFPGYPDGVTGTQMMEDLKKQAERFGTDVRFGI
                     ATAADLSSMPYRITIDDEKVVETDALIIATGATAKYLGLESENKFKGQGVSACATCDG
                     FFYKGKDVAVVGGGDTAAEEATYLAGLCRKVYLIVRKSYLRASKAMQEKVASTPNIEI
                     LFEHTTKEIVGDQSGVNGVILTNTKTGKDVKVDISGFFLAIGHHPNSELFKPWLDTDE
                     VGYIKTLPDSTKTNLPGVFACGDVRDPHYRQAITAAGSGCMAAIDAERYLGERKF"
     gene            3684..4688
                     /gene="gap"
                     /locus_tag="PK839_08505"
     CDS             3684..4688
                     /gene="gap"
                     /locus_tag="PK839_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018335837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HOG72814.1"
                     /translation="MGKIKVAINGFGRIGRNVFKLALERPELEIVGINDLTDTKTLAY
                     LLKYDSTQGRFEGKVEFDAENLIVNGKKYKISAERSPLNIKWMETPDVVVESTGIFTK
                     RESEKGGYGDHIKNGAKKVVLTVPAKDEIDRMVVVGVNEDTLKPTDQCISNASCTTNC
                     LAPVVKVLNDSFGIERGYMTTIHSYTNDQRILDAPHSDLRRARSAAVSQIPTTTGAAK
                     AVGKVIPELKGKLDGISVRVPTPTGSLVDFVAILKKEVTKEDINAAMKKAAEGPMKGV
                     LEYTEDPIVSADIIHNPHSSIFDAQSTMVNGNMVKVLSWYDNEWGYSARVVDLIPKLF
                     "
     gene            4930..5217
                     /locus_tag="PK839_08510"
     CDS             4930..5217
                     /locus_tag="PK839_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007655885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015661 - L-lysine efflux transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903401 - L-lysine transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysO family transporter"
                     /protein_id="HOG72815.1"
                     /translation="MITVVAIMAIGILVGYLLRQKRKLIGFLERLTTWAIYLLLFLLG
                     IAIGTNEEIIRNLPTLGGRAFIISVAGVLGSILLSWATYRLFFKKDEEKQE"
     gene            5214..5822
                     /locus_tag="PK839_08515"
     CDS             5214..5822
                     /locus_tag="PK839_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005678698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine exporter LysO family protein"
                     /protein_id="HOG72816.1"
                     /translation="MRGSLTILAFFAAGILLGFKDMLPQWLTATDLSSYALYLLMFLV
                     GIGIGGDDRALKAIKGFGLHIFLVPLTTIVGTLTGVAAVYLLLGQEPFTDMLAVGAGF
                     GYYSLSSIIISEIRGPELGVVALLANVMREIITLLMAPIFARYFGKLAPISSGGATSM
                     DTTLPIITASSGKEYAIVSLFHGIVLTILVPFLVTFILSLQS"
     gene            complement(6071..7318)
                     /locus_tag="PK839_08520"
     CDS             complement(6071..7318)
                     /locus_tag="PK839_08520"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG72817.1"
                     /translation="MKYAALINETFRISIRSITSTKLRSALTILIIALGIMSLVGIIT
                     AIKVIENSISTGFASMGANSFTIQSRGMHVNVNGNQYRTKNYSFISLQQAREFKDKFD
                     FPANVSVAVYATGASTIKYLSNKTNPNISVRGVDENFMMVSGLDVTQGRNYSVTEVND
                     GRNVAIIGNEVARKLFPKNEDPIDKFISVGSGKYRVIGVLKSKGAGFGGGPDRTVFLP
                     YTNVAAYFSRPNMNYSITVQPHDPKLMDAAVGEAEGIFRLVRRLSATDESDFNIEKSD
                     TLAKLLIDNLKFVSIAATLIGIITLVGAAVGLMNIMLVAVAERTREIGTRKAIGAKSP
                     TIKQQFLFEAILICEIGGLLGVFLGILVGNLVSVLAKTSFVVPWGWIFGGLAISFLVG
                     LASGYIPAVRASRLDPIEALRYE"
     gene            7637..8683
                     /locus_tag="PK839_08525"
     CDS             7637..8683
                     /locus_tag="PK839_08525"
                     /inference="COORDINATES: protein motif:HMM:NF017686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0008218 - bioluminescence [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HOG72818.1"
                     /translation="MKIEERLNAFARLGDKFTKTLSCNEESELGAAIEKAHEQNPWFT
                     PNNVRFALATIAESWLKPDVLNSWIARYPEACFNPQHPVNVGVIMAGNIPLVGFHDFL
                     CVLATGNRITCKLSARDGNLMRSVANTLIQLEPRFSNLIAITDGTLRDFDAVIATGSN
                     NSARYFDYYFGKYPSIIRGHRNGIAVLTGKETQSNLTKLGEDIFTYFGLGCRSVSKLL
                     VPSGYSFTPLLDSFCQWKDLINHHRYANNYEYQRAIFTINQTPHFDTGFLLVTPSEQL
                     DSPVGVLYYQEYNKLDDALKYIGQHDKVLQCVVGLPEIYPKAIPFGSAQNPDIAEYAD
                     EIDTIVFLSQLQAL"
     gene            8741..9259
                     /locus_tag="PK839_08530"
     CDS             8741..9259
                     /locus_tag="PK839_08530"
                     /inference="COORDINATES: protein motif:HMM:NF019542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72819.1"
                     /translation="MIYKFRMISSEDRAFLRDFEVDANQTFLTFHEAIQGNLNFDPSQ
                     LVSFFLADEHWNKGMELTLIDMQNEGDMAAIPMDSATVGELIKNRKERLLYVFDIYTD
                     RSLFIELLDIYEPTPGVTYPICTAAVGDPPLQFAGSDIENFSPELADDAGFEDFSNEF
                     DSDEAGSDSIDD"
     gene            9337..10266
                     /gene="miaA"
                     /locus_tag="PK839_08535"
     CDS             9337..10266
                     /gene="miaA"
                     /locus_tag="PK839_08535"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005935983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOG72820.1"
                     /translation="MSYNGNFLVILLGPTGVGKTDLSISIAKHFEAPIISADSRQFYQ
                     EMRIGTAAPTPSQLAGANHFFIGNKSITERYSCGMFEVDALNLLETVFTSSRIALLVG
                     GSGLYIDAVVNGIDDFPTPDPELRQALLSQLKNEGVASLRAQLKILDPEYYGKVDLRN
                     SQRVLKALEVCLQTGLTYTSFLTHRAKPRFFNSIFVGLNRPREELYRLINQRVESMMA
                     EGLLDEAKKLYPFRELNALNTVGYRELFSYLDGQTSLEEAIELIKRNTRRYAKRQLSW
                     WSRNKNITWFHPSQLTEITEFIEKTIASYSAKN"
     gene            10273..10989
                     /locus_tag="PK839_08540"
     CDS             10273..10989
                     /locus_tag="PK839_08540"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOG72821.1"
                     /translation="MISNEKPNSEIFIELFTEIEQRLKTICNDEFTSNFSELLAKARG
                     KSGVVNRYASDLKEFAQLRNAIVHTRRQNFIIAEPHDDVVSEVIHIHKLLSSPPKVSS
                     VMRHNPFTVFPETPISVVLNTFAEKGFMRCPVVEQGRIVCLITAKTISRWITSIKQDR
                     IEVNNTPVRKLVPFADKSDYMVIAENDDIATLLDLFNESIRKGSYLQAALVTQSGEPQ
                     SKLVGIVTPSDLPLIMEKLK"
     gene            11143..13131
                     /locus_tag="PK839_08545"
     CDS             11143..13131
                     /locus_tag="PK839_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HOG72822.1"
                     /translation="MSDEPVEKIKGLPKNAYTELKDGEEYIPIMPPHKTVPEVNLWSV
                     TWGLVMAIIFSAAAAYLGLKIGQVFEAAIPIAIIAVGLSTMGKRKGALGENVIIQSIG
                     ASSGVIVAGAIFTLPALYILNLEAQFYQIFLSSLFGGILGILFLIPFRKYFVSDMHGK
                     LPFPEATATTEVLVSGEKGGKQARLLVVSGLIGGIYDFIIATFGWWSEVITSRVIPWG
                     EALADKSKLVFKLNVGAAVTGIGYIIGLKYTAIIAAGSFVGWFVIIPIINYFAPGLTA
                     AVGSNITAVVGSMSAEDIFTNYVRPIGIGGIAMAGIIGIINSSAIIRKALGLVVSELF
                     GKHKSMPKEELRTRRDLPMYIIAGGILLTTILILIFFHFGVVHNLAHALIGLAIVLII
                     SFLFTTVAANAIAIVGTNPVSGMTLMTLIITSLVMASVGLSGTSGMVAALIIGGVVCT
                     ALSMAGGFITDLKIGYWLGSSPFKQQTWKFLGTFVSAATVGGVIMLLNKTYGFTGKDA
                     LVAPQANAMAAVIEPMMSGKPAPWLLYAAGAFFSLILTMIKVPALPFSLGMFIPLQLN
                     TPLLVGGIISHIVSTRSKDAAVNNARKDRGTLVASGFIAGGALMGVISAILRFFNVNY
                     VNAQWFESHSAELLGLVMFLILCGYLIWESLRAKPEGE"
     gene            13329..15269
                     /gene="dnaG"
                     /locus_tag="PK839_08550"
     CDS             13329..15269
                     /gene="dnaG"
                     /locus_tag="PK839_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005802118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HOG72823.1"
                     /translation="MIEQQVVDRIIAAADILEVLQDFVTLRKRGVNYLGLCPFHNEKT
                     PSFTVSPSKGIYKCFGCGKGGNVVNFIMEHEHLGYVDALKFLAKRYNIEIVEKEATPE
                     ELEQRNERESLMVVSAYAQKYFSNILHKHSDGKNIGMAYFRERGFHDNIIEKFQLGYS
                     LDQRNAFTRTALHDGYKLDYLVKTGLTIEGKDGNPFDRFAGRVMFPIHSLSGRVIGFG
                     GRVLRTDTKTAKYLNSPESEIYHKSEVLYGIFQAKKAITQEGKCFLVEGYTDVISLHQ
                     AGVENVVASSGTSLTHDQIKLIKRFTSNVTILYDGDAAGIKASLRGIDMVLEEGLNVK
                     IVLLPDGEDPDSFARSHSASEVLKFIDEHETDFIRFKTLLLLDDAKNDPIKRANLIGD
                     IVQSISVIPEVIKRSVYIKECSRLMDIEENVLYTEVNRQRRKRFEQLVQRESREPKEK
                     ETPRLPSYVSGVVCEEQEKEIISFLLNYGSKTLYNSQSEENKDDEINITVGQYIIDEI
                     LNDELEFQNLAYKMIFDEYAQLLSQNQAVDNRHFINHYNPQISQLAVDLMSSRHKLSR
                     IWEKHKAEVTDDPDFLLHAVPKAIQVYKSKVLKIAIKNLTDELTHLSAQQKDETDEIL
                     KRLLELNKLMNSMSKGLDRVVL"
BASE COUNT         4318 a         3637 c         3432 g         3946 t
ORIGIN      
        1 caaactcagg gcaggctcat tcgacaggtt tagtgcaaac ccgaaactct ttgctagttc
       61 attaatttat tcaaacctgc agcgaggttt attttaggtc ttaacttttt agttcaaaga
      121 tcgttaacct ctatgcttcc gttgaataca tgactaacag cgccttcaag ccaaatattc
      181 ttgtacccat cgccctcatg gctaaaatat accctgaggg ttccacctgg ggcaagcagc
      241 tcaactggcc cttcggaaat cgcaaaaaaa tcagcatatg caattgctga agccactgag
      301 ccggttccgc atgaaagggt ttctgcctcc actccccgct catacgttct tatcttcaat
      361 accttctcac ctaccggctg aataaaatta acgttggttc caccattctt cacatttacc
      421 gacttgctgg caagcctacc ctcaaggtca acgttaacgt tctcaacact acgtacaaaa
      481 acgacaaagt ggggcgaacc ggtattgagg aaaaagcctt cctcagcaga ctcaacaacc
      541 ggtacatcta tcatacgtaa ccgaatgttt ccattatcga gcaggtaggc ttcgtgaaaa
      601 ccatcaattc cgttgaagag cacatgctgc ccggctaacc caagctggtg ggcgaaatgg
      661 gcaaaacacc ggccgccatt cccgcacatg gttgactcac gcccatcgga gttgaagtag
      721 cgcatcatga gagcactctc cttggcgttc tccagaagaa ttagcccatc agctccaatt
      781 ccaaaatgcc tgttacagag cttttcaatc aagtcaactg tagggtttaa ccctcctgag
      841 cggttgtcaa ccattacgaa atcgttgccc gttccgtgat atttcgagaa ggttatcagc
      901 atagcacgtg tgaattttta aaatttttgg tgaaagttag gtgatttttt ccaataatgt
      961 tgacatcggg ttaagacctc ttccccaatc ggtcatatcc caattagaat gcaggagtaa
     1021 gagaacattc atcaacttca ccccaccaac aggtaacaaa aaaagccccg cacagggggc
     1081 ttttagttat caaacaaatc atccagcaaa ataggctaac ctagataaga cttcaacagc
     1141 ttactccgtg aggtatgacg aagtctccgt atggcttttt ccttaatctg acgtacccgt
     1201 tcacgggtaa ggttaaactt ctcgccaatt tcctccaggg tcatctcctg gcagctgatg
     1261 ccaaaaaaat acctgacaat atcacgttcg cgctcggtaa gggtggctag tgccctttca
     1321 atttccttgc tcaacgactc gttaattagc cccttatcgg catttggaga atcgttatta
     1381 acaataacat caagcaggct gttatcctcg ccatctacaa aaggtgcatc tacggaaaca
     1441 tggcgaccac tgatacgaag ggtatcgtta attttatcct ttgagatatc aagcagctcg
     1501 gccagctcct caggtgaggg agttctttca tactcctgtt caaacttaga aaaagccttg
     1561 ttaatcttat ttaacgaacc cacctggttt aggggcaggc gaacaattct ggactgttct
     1621 gccaaagcct gaagaattga ctggcgaatc caccaaactg cataggatat aaacttgaat
     1681 ccccgggttt cgtcaaattt ctcggcggct ttaattaacc ccaaattccc ctcgtttata
     1741 aggtcgggca ggcttaaccc ctgattttga tactgcttgg ctacagaaac aacaaatcga
     1801 aggttggctc gggttaattt ctccagagct gcctggtcgc ccttcctgat tcgctgggcc
     1861 aactccacct cttcctcaac cgtaatgagg tcctccttcc caatttcctg taagtactta
     1921 tcgagcgatg cactctcccg attagtaatt gattttgtga tttttaactg tctcattcga
     1981 aaccttctat ttattttgca aagttatggc tcttcaacat gcaattcaag tttatgcagc
     2041 aaaaaataat gactgcgaaa ctacttagct caacttctat ataatgcttc cctaaaacgg
     2101 ttggcatttt gaaattgtta cattattccg gtcgaatatt cttaattact attaaaaatt
     2161 tagcttgatc cgattcaggc ttaccgaaaa tggaggtgta tttccaaaac aaaaaagcct
     2221 taagcacctt tattgctaca acatttctca aggatatttg ttgtgagttg gctgcgcctt
     2281 aatagctact gagtagcgat atggcctttt ttacacttta aaaacatgaa attagcagac
     2341 taaaagcagt agatttgtaa ccatcaatat taaaatttca aaagaataag ctatgaacct
     2401 attcactcct ttaaattccg aaagcggaaa aaatttatcc ggatcaccaa agccggttga
     2461 gcatgttagg gtgctaatac taggctcggg gccggcaggc tatacagcag caatatatgc
     2521 tgcacgagcc aaccttagcc ctgtggttta cgagggtatc gaacctgggg ggcaactaac
     2581 caccaccacc gaggttgaaa attttcctgg ctacccggat ggggttaccg gcacacagat
     2641 gatggaagat ttgaaaaagc aggccgaacg ctttggaacc gacgttcgat tcggaatagc
     2701 cacagccgct gacctttcaa gtatgcccta ccggataacc atagatgacg agaaagttgt
     2761 agaaaccgat gcgcttatca tagctacagg ggcaactgcc aagtacctag gtctggaatc
     2821 ggagaataag ttcaaggggc aaggcgtttc ggcatgcgcc acatgcgacg gatttttcta
     2881 caagggaaaa gatgttgccg ttgtgggtgg tggtgatact gcagccgaag aggcaactta
     2941 tcttgccggc ttatgccgta aggtttacct gattgttcgt aaatcatacc tgcgcgcatc
     3001 aaaggcaatg caggaaaaag ttgccagcac tccaaacatc gaaattcttt ttgaacacac
     3061 caccaaggag attgtaggcg accagtcggg ggtaaacggg gtgattctta ctaataccaa
     3121 aacaggaaag gatgtaaagg ttgatatttc gggatttttc ctggccattg gccaccatcc
     3181 caactccgaa ctctttaagc catggctgga tactgacgag gtgggctaca tcaaaaccct
     3241 gcccgattca accaaaacaa atctgcctgg tgtatttgcc tgtggcgacg ttagggatcc
     3301 gcactaccgc caagccatta cagctgccgg ctcgggatgc atggcggcta ttgatgccga
     3361 acggtacctg ggtgagagga aattttagtt tggttttatt cggcataaaa tctaaaactg
     3421 tccttgtctg ggttaaaaca ccctctacaa aaactaccta aacaaagaaa ccatcaaatt
     3481 attaatattt tttagcaatt gcggcaaaat tttgaatttc atcgcaaacg gttgaataca
     3541 aattggtaga gcatgctgtt ataagtggta tgccaattag gtgatttgac aagttgaaaa
     3601 aaggggcggt aaatgtaatt ttagcttatc tttgcttcac gaaaagggtt aacatttaca
     3661 taatcaacta atacgaagta gatatgggaa aaattaaagt agcaatcaac ggattcgggc
     3721 gaattggccg taacgtgttt aaactcgcac tggaacgtcc cgaacttgaa attgttggta
     3781 ttaacgattt gaccgacacc aaaaccctgg cctacctact aaagtacgac tctacacaag
     3841 ggcgctttga aggcaaggtt gagtttgacg ccgagaacct gattgtcaac gggaaaaagt
     3901 ataaaatatc agccgagcgc tctccactaa acattaagtg gatggaaaca cctgatgttg
     3961 ttgttgaatc aaccgggatt ttcaccaagc gtgagagcga aaagggtgga tacggcgacc
     4021 atattaaaaa tggcgccaaa aaggttgttt taacagtccc cgccaaggac gaaattgacc
     4081 gaatggtagt ggttggcgtg aatgaggata ctcttaaacc aaccgaccag tgcatttcca
     4141 acgccagctg caccaccaac tgcctggctc cagtggtgaa ggttttaaat gacagtttcg
     4201 gaattgaacg gggttacatg accaccattc actcctacac caacgaccag aggattcttg
     4261 atgcacctca cagcgacctt cgcagagccc gttcggcagc cgtttcccaa attccaacaa
     4321 ccaccggtgc tgccaaggct gttggcaagg ttatccctga gttgaaaggt aagcttgacg
     4381 gaatatccgt tcgcgtccca accccaaccg gttcgctggt tgactttgtg gccattttga
     4441 aaaaagaggt aaccaaggag gatattaacg cagccatgaa gaaagctgcc gaaggaccaa
     4501 tgaagggcgt tcttgagtac accgaggacc ccattgtttc agctgatatt attcataacc
     4561 cccactcctc catctttgat gctcaaagca ccatggtcaa cggcaacatg gtaaaggtgc
     4621 tttcttggta tgataacgaa tggggctact cagccagagt tgtagatttg attccaaagc
     4681 tattttagcc atacctagcg ataatgaagg gctacccgcc attgggtagc cttttttaat
     4741 tttgtaaagt acacctattt gattgcattt catgattgga gaataaattc aacatctgaa
     4801 ttcataagca aaattcagac taaaaaaaga gtacttttcg attttatacc ccacccaata
     4861 ttttacgctt gcaagtgaat ggtgcttcaa aatgtatagt cggaaagtag atttaaagtt
     4921 tcatagcaaa tgattacagt tgtagcaata atggccattg ggatactcgt tgggtatctc
     4981 ctgcgtcaga aaagaaagct gattggattt ctcgaaaggc ttacaacgtg ggccatatac
     5041 ctgctgcttt tcttactagg aatagcaatt ggaactaacg aggaaatcat aagaaacctg
     5101 ccaacgctgg gtggcagggc gttcataatc agcgttgcgg gtgttctggg cagcattcta
     5161 ctttcgtggg cgacctaccg cctgttcttc aaaaaggatg aggagaagca agaatgaggg
     5221 gtagtcttac catactggct ttttttgcag caggcatact gcttggtttt aaggacatgc
     5281 ttccccaatg gttgactgct accgacctga gcagctacgc gctctacctt ctgatgttcc
     5341 tggtgggaat aggaataggc ggcgacgaca gggcgctcaa agccatcaag ggattcggcc
     5401 tgcatatttt tttggttccg ctcaccacta ttgtgggaac gctaacgggg gtagccgctg
     5461 tttaccttct gctgggacag gaaccattta ccgacatgct tgccgttggt gccggattcg
     5521 gctactatag cctttcgagc atcatcatca gcgagataag gggtcccgag ctgggagtag
     5581 tagccctgct ggccaacgtg atgcgcgaaa tcataacgct gctcatggcg cccatatttg
     5641 cccgatactt tggcaagcta gcccccatat catcaggcgg ggcaaccagc atggatacca
     5701 cccttccaat cattacggcc tcatcgggca aggaatacgc catcgtatcc ctgttccacg
     5761 gaattgttct caccattctg gtcccctttc tggtaacgtt tatcctttcg ctgcaaagct
     5821 gaaattgaca ccaaccctac aaataaggaa gtatattttg gcttttgttg tttcctggaa
     5881 atacctagct attaagggaa caaatcaaaa acagcaatgg aattgaggct aaatgtttac
     5941 ggattacaga gattagggag atgcacgtag gttctgtcag tacaaatttc cctaataccc
     6001 tccgttttta ccgtggttaa attcccctct accataacat aaagggaaat aagcaaaaag
     6061 ctagatgccc ctactcgtaa cggagcgctt cgatgggatc aaggcgcgaa gccctaaccg
     6121 caggaatgta acccgacgct aaaccaacca aaaaggaaat ggccagccct ccaaaaatcc
     6181 atccccaggg aacaacaaag gaggttttgg caagtaccga caccaggttt cctaccagga
     6241 tgcccaggaa aacaccaagc aacccaccaa tttcgcatat cagaatagcc tcaaaaagga
     6301 actgctgctt gatggtgggt gattttgccc ctattgcctt gcgagtacca atctccctgg
     6361 ttctctcggc aaccgccaca agcataatgt tcatcaaacc aacggctgct ccaaccaggg
     6421 ttattatccc aataagggtt gctgcaatgg aaacaaactt gaggttatct atcagcaact
     6481 tggccagcgt gtcgcttttt tcaatgttga aatcggactc atcggtggcg gaaagcctgc
     6541 gaaccagcct aaaaatacct tcggcctcgc caacagctgc atccatcagc ttagggtcgt
     6601 ggggctgaac agttatgctg tagttcatgt tgggacgtga aaaatacgcc gcaacattgg
     6661 tgtagggcag gaaaactgtt ctatcgggac caccgccaaa accagcccct ttcgatttca
     6721 gaacccctat cacccggtac tttccgctgc ccacgctaat gaacttgtca attggatctt
     6781 catttttggg aaaaagctta cgggctacct cgttaccaat tatggcaaca tttcttccat
     6841 cgtttacctc ggtaaccgaa tagttccgac cctgcgtaac atccaggccc gacaccatca
     6901 taaagttttc gtcaacaccc ctgacgctta tgttggggtt ggttttgttg gataagtact
     6961 tgattgtgct agcccctgtt gcgtaaacag ctacgctgac attggctgga aaatcaaatt
     7021 tatccttgaa ctccctcgcc tgttgaaggc tgataaagga gtagttcttg gttcgatact
     7081 ggttaccgtt tacgtttaca tgcatccccc tgctctgaat ggtaaacgag tttgccccca
     7141 tgctggcaaa tccagtgctt atgctgttct caatcacctt aatggcagta attattccaa
     7201 ccagcgacat gatacccaac gcaattatca gtatggtaag cgcagaccgg agctttgtag
     7261 aagtaataga ccggattgaa attcgaaatg tttcgttgat tagggcggcg tacttcacaa
     7321 cacttaaatt aggttaaaca aatagtcagc acctcatttt ctcatgaaaa cataaaacaa
     7381 catcgtctta atgcgaagct ataatttgat gggtgtccca catcaaatcc ttttcttcta
     7441 aagcattgct gtttttcata tgctgcctgt tccgttgaaa aactttcaaa gttattaatt
     7501 tataccaatt ttcgtccgaa aagttaaggt caatacaaaa cccttgcagc aggatgaagc
     7561 ctaccctgca cagctcaaac attgcggtcc gagccaaaat aatactagct ttgcaacata
     7621 aagacgcagt gttaagatga aaatagagga aagactaaac gcgtttgctc gtttgggtga
     7681 taaatttacc aaaacgctat cgtgcaatga agagtcggaa ctgggtgctg ccattgaaaa
     7741 agcgcacgag caaaatccat ggtttacccc caataacgtt cgatttgctt tagctactat
     7801 tgccgaaagc tggcttaaac ccgatgtcct taacagctgg atagcgcgct accctgaagc
     7861 ctgcttcaac ccgcagcatc cagttaacgt tggtgtaatc atggccggga atatacctct
     7921 tgttgggttt catgatttcc tttgcgtgct ggcaaccggt aaccggatta cctgcaagtt
     7981 gtccgcaagg gatggcaacc tgatgaggtc tgtggcaaat accctgatcc agcttgagcc
     8041 gcgcttttca aaccttatag ccatcaccga cggcacacta agggattttg atgcagtaat
     8101 tgctacgggt agcaacaact ccgcccgata ttttgattac tactttggga agtacccatc
     8161 tattatcagg ggccaccgca acggaatagc cgtgctaaca ggaaaggaaa cccaaagcaa
     8221 tttaacaaag ctaggagagg atattttcac ctactttggc ctgggctgcc gaagcgtttc
     8281 caagctattg gttccatcgg ggtatagctt tactcccctg ctcgacagct tttgccagtg
     8341 gaaagaccta atcaaccacc accgctatgc caataactac gagtaccagc gtgcaatttt
     8401 caccataaac caaacgcccc attttgacac cgggtttctg ctggtaaccc cttcagagca
     8461 actcgactct ccggtggggg tactttacta ccaggagtat aataaacttg atgatgctct
     8521 gaagtacatt ggacagcacg ataaagtatt gcaatgcgtt gtaggattac ccgagattta
     8581 cccaaaagcc attcccttcg gatcggctca aaacccagat attgcggaat atgcagacga
     8641 gatcgacaca attgtatttc tttcacaact gcaggctctt taactttgaa tatttgcatt
     8701 tattcactaa aactgcttat tttgtagcaa agtataaagt atgatataca aattcagaat
     8761 gatatcgtcg gaggacaggg cattccttcg ggattttgag gttgatgcca atcaaacctt
     8821 cctaaccttt catgaggcaa ttcagggaaa cttgaatttt gacccatcgc agctggtatc
     8881 attctttctt gccgatgaac actggaataa aggcatggaa cttaccctaa tagacatgca
     8941 gaatgagggt gatatggctg ctattcccat ggattctgca accgttggcg agctgataaa
     9001 gaatcgcaag gaacgcctgc tttacgtttt cgacatatac accgatcgta gcctatttat
     9061 cgagctgctc gacatttacg agccaactcc cggagttacc taccctatct gcactgcagc
     9121 agtgggcgat cctccacttc aatttgcagg tagcgacatc gaaaatttca gccccgagtt
     9181 ggccgatgat gctggattcg aagatttttc caatgaattc gattccgacg aggctggtag
     9241 cgactccatt gacgattaac ccgcataaac tcggtataaa agcttcataa agcatagctg
     9301 aaacgagggc ggttttagcc gcttccatga aaaataatga gctataacgg caattttttg
     9361 gtaatcctgc ttggccccac aggagttggg aaaacagacc tgagcatttc cattgccaag
     9421 cactttgaag cgcccattat atcagctgat tcacgtcagt tttaccagga aatgaggatt
     9481 ggaactgctg cacccacccc cagccagctg gccggagcta accacttctt tattggcaac
     9541 aagtctatta ccgagcggta tagctgtgga atgttcgaag ttgatgcgct caacctactt
     9601 gaaaccgttt ttacatcaag cagaattgcc ctactggtag gaggttcggg gctttacatc
     9661 gatgctgtag ttaacggaat tgacgatttt cccactcctg accccgagct acggcaggcc
     9721 ttactcagcc agctaaaaaa tgaaggggta gccagcctgc gagcgcagct aaaaattttg
     9781 gatccggagt actatggaaa ggttgacctg aggaattccc agcgcgtgct aaaagccttg
     9841 gaggtttgcc tgcaaactgg tctcacctac acctcattcc ttacccatcg ggcaaagccc
     9901 agattcttca attcaatttt tgtggggctt aaccgtccac gggaggaact ctaccggcta
     9961 ataaaccaaa gggttgaatc catgatggct gaggggttgt tggacgaagc aaaaaaactt
    10021 taccctttta gagaactcaa tgcccttaac accgttggct accgcgagct attcagctac
    10081 ctcgacgggc aaacaagcct cgaggaggct attgaactta tcaaacgaaa tacccgacgc
    10141 tacgccaagc gtcagctaag ctggtggagc cgaaataaaa atattacctg gttccatccc
    10201 agccagctga ctgaaattac ggaattcatc gaaaaaacca ttgccagcta ttcagcaaaa
    10261 aattaaaaaa atatgatctc gaacgaaaaa ccaaactccg aaatttttat cgagctgttc
    10321 accgagattg agcaaaggct caaaacgatt tgcaacgacg agttcaccag caacttctcg
    10381 gagctgttag cgaaagcacg tggcaagagt ggtgtggtaa accgttatgc atctgattta
    10441 aaagagtttg cccagctacg caatgccatt gttcacaccc gccgccaaaa cttcataata
    10501 gccgaacccc acgatgatgt ggtgagcgag gttatccata tccataagct gctaagtagc
    10561 cccccaaaag tatcgtcggt tatgaggcat aatccattta ccgttttccc tgaaacacca
    10621 attagcgttg ttctaaacac ctttgctgaa aaaggattca tgcgctgccc tgtagtggag
    10681 cagggtagaa ttgtttgcct gatcaccgct aaaaccattt cgcgttggat cacctccatt
    10741 aagcaggata ggattgaagt gaataacacc ccggttagaa agctcgtccc gtttgccgac
    10801 aaatcagact atatggttat agctgaaaat gacgacattg ctactcttct cgacctcttc
    10861 aacgaatcca tcaggaaggg aagctacctt caggcagcgc tggtaaccca aagcggcgaa
    10921 cctcaaagta agctcgttgg gattgttacg cctagcgacc taccacttat catggaaaaa
    10981 ttaaagtaga accgggcagt agagtttcga gcgctttgat gaggagtttc ctcagaatca
    11041 cgctcgtaat aagaagttga atcactgaaa agatttactt tttttcttct atttttgcca
    11101 actagctaat catcctacct aaaatcttaa aaataaaaaa acatgagcga cgaaccggtt
    11161 gaaaaaatta aagggctccc taagaatgcc tacaccgagc taaaggatgg cgaggagtac
    11221 attccaataa tgccgcccca taaaactgtt cccgaggtta acctctggag cgtaacatgg
    11281 ggactggtaa tggccataat attctcggca gcagcggcct accttggact aaaaatcggg
    11341 caggtttttg aagcggccat tcccattgcc ataattgccg taggcctgtc aaccatggga
    11401 aaacgaaagg gtgctcttgg tgaaaacgtc atcatacaat ccattggagc cagctcgggg
    11461 gtaattgttg caggtgccat attcaccctt ccggcgcttt acatcctaaa cctcgaggcg
    11521 caattttacc agattttcct atcgtcgctt ttcggaggaa ttcttggcat ccttttcctg
    11581 ataccgtttc gaaagtactt tgtatccgac atgcacggaa aacttccgtt tccggaggca
    11641 acagccacta ccgaggtgct ggtatcgggc gaaaagggag gcaagcaggc gcgcttgctt
    11701 gtggttagcg gtctaatagg tggtatatac gattttataa ttgccacatt cggttggtgg
    11761 agcgaggtga ttacctccag ggtaatccca tggggcgaag ccttggccga caagtcaaaa
    11821 ctcgtattta agttgaatgt aggtgctgcc gttacgggga ttggatacat catcggactg
    11881 aagtacactg ctatcattgc agcaggctcg ttcgttggtt ggtttgttat tatccccatc
    11941 atcaactatt ttgcccctgg gttaaccgca gcagtaggaa gcaacataac cgcagtggtg
    12001 ggaagcatga gcgccgaaga tatttttacc aactacgttc gaccaatagg aataggcggc
    12061 attgccatgg ccggaatcat cggaatcatc aactcaagcg ctatcatacg taaagccctt
    12121 gggttggtgg tttcagaact ctttggaaag cataaatcca tgcccaaaga ggaactccgt
    12181 acacgcagag acctaccaat gtacattatt gcaggaggca tcctgctaac caccattctc
    12241 attcttattt tcttccactt tggcgtggtt cataaccttg ctcacgccct gatcggtctg
    12301 gccatagttc ttataatatc cttccttttc accaccgtag cggccaacgc aatagccatt
    12361 gttggaacca accctgtttc ggggatgacc ctcatgacgc tcatcatcac ctcgctggta
    12421 atggcttcag tagggctttc gggaacgtcg ggaatggtag ccgcactcat tataggaggc
    12481 gtggtttgca ccgccctgtc aatggcagga ggtttcatca ccgatttgaa aatcggctac
    12541 tggttgggtt catcaccttt taaacagcaa acatggaagt ttcttggtac tttcgtatcg
    12601 gccgccacgg ttggcggagt tatcatgctg ctgaataaaa cttacggttt tacaggcaaa
    12661 gatgcgctgg tggccccaca ggctaacgcc atggctgccg ttatcgaacc tatgatgagc
    12721 ggtaaacctg ctccctggct gctctacgct gccggagcat tcttctccct gatacttacc
    12781 atgattaagg ttcccgccct ccctttctcc cttggtatgt tcatcccgct acagcttaac
    12841 accccattac tggtaggtgg aataatttcc catattgttt ctacacgctc aaaggatgct
    12901 gctgttaaca acgctcgtaa ggacaggggc acgctggttg cctctggatt catcgccgga
    12961 ggcgcattaa tgggtgttat tagcgccatc ctgagatttt ttaatgtgaa ctatgtaaat
    13021 gcccaatggt tcgaatcgca ctcggcggaa ctgctcgggc tggtaatgtt cctgatactc
    13081 tgcggctacc tgatttggga gagccttagg gccaaacccg aaggggagta gcaactcatt
    13141 tctatactct cgatagcggc taccctgcca tcttgctggc tgtgtatccg ctatcttttt
    13201 gttatccagc atgttatatg gccaaagcaa ccaatatgca aagctgcttt tttaacaatt
    13261 ctatcggtca aaaaggaatg aaaaaaacat caaagttcct acctttacca tagaaataaa
    13321 gcagaaagat gattgagcag caagtagttg acaggataat tgccgcagca gacatactgg
    13381 aggtcctaca ggatttcgtt acccttcgta aacggggagt taactacctc ggactatgcc
    13441 catttcataa cgaaaaaaca ccttcgttta ccgtcagccc atcaaaggga atctacaagt
    13501 gctttggttg cggaaagggc ggtaatgtgg ttaacttcat catggagcat gagcatttgg
    13561 gctacgtgga tgcgctaaag ttcctggcca aaaggtacaa tatcgagatt gttgaaaagg
    13621 aggctactcc cgaagagctg gaacagcgaa acgagcgcga aagccttatg gtggttagcg
    13681 cttatgcaca aaagtacttc agcaacattt tacataagca ttccgatggc aagaatatag
    13741 gaatggccta ctttcgtgaa cggggattcc acgacaatat tatagagaaa tttcagctgg
    13801 gctatagcct cgaccagcga aatgccttta ccagaacagc cctgcatgat ggttacaagc
    13861 tggattacct ggtaaaaacc gggctgacca tagagggcaa ggatggtaac cctttcgaca
    13921 ggtttgccgg tagggtgatg tttccaatcc acagtctgag cggacgggtg attggttttg
    13981 gcggacgcgt tcttagaacc gacactaaaa cagctaagta ccttaatagc cctgaaagcg
    14041 aaatatacca taaaagcgag gtactctacg gaatctttca ggccaagaag gcaataaccc
    14101 aggagggtaa gtgtttcctg gtggaaggct acaccgatgt tatttcgctc caccaggcag
    14161 gtgtggagaa tgtagtggcc agttcaggta catcgcttac ccatgaccag attaagctca
    14221 tcaagcgctt caccagtaat gttaccattc tctacgatgg agatgcagct ggtattaagg
    14281 cttcattaag gggtatcgac atggtgctgg aggagggtct aaacgtaaag attgtgctgc
    14341 tccccgacgg cgaggacccc gacagctttg cccgttcgca tagcgcttcc gaagtgctca
    14401 agtttattga tgagcatgag accgatttca tccgcttcaa aacactactc ctactcgatg
    14461 atgctaaaaa tgatccgata aaaagggcaa acctgattgg cgatatagtg cagtccatca
    14521 gcgtgatacc cgaggttatc aagcgttcgg tttacattaa ggaatgctcc cggctcatgg
    14581 atatcgagga aaatgttctc tacaccgagg tgaacaggca gcgccgcaaa cgcttcgagc
    14641 agctggttca acgcgaatca agggaaccaa aggaaaagga aacaccacgt ttaccttcat
    14701 acgtatcagg tgttgtttgc gaggagcagg agaaggaaat tatcagcttc ctgctgaact
    14761 atggcagcaa aacgctctac aacagccaat cagaggaaaa taaggacgat gagattaaca
    14821 ttacagttgg ccagtatatt attgacgaga tactcaacga tgagctggag tttcaaaacc
    14881 tggcctacaa gatgattttt gacgagtatg cccagctgct ttcccagaac caggctgttg
    14941 acaaccgaca cttcatcaac cactataacc cacagataag ccagctggcg gttgacttga
    15001 tgtcgagcag gcacaagttg agccggattt gggaaaaaca taaggccgaa gttacagacg
    15061 atcccgattt cctgcttcat gccgtgccaa aggctattca ggtttacaag agcaaggtgc
    15121 ttaagattgc cattaaaaat ttaaccgatg agctgaccca cctatcagcc cagcaaaaag
    15181 atgaaacaga tgaaatactc aagcggctac tcgagctaaa caagcttatg aactccatgt
    15241 cgaaggggtt agacagggta gtgctttaga aacagcagaa aagtaaacag gaaacaagga
    15301 taaagagatt tcaaggtaaa ggattcctga ttc
//
LOCUS       DAOSHM010000058        14508 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_3428_length_14508_cov_19.632395, whole genome shotgun
            sequence.
ACCESSION   DAOSHM010000058 DAOSHM010000000
VERSION     DAOSHM010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 14508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14508
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3428_length_14508_cov_19.632395"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(101..1969)
                     /locus_tag="PK839_08555"
     CDS             complement(101..1969)
                     /locus_tag="PK839_08555"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG72824.1"
                     /translation="MKRILVGSTLVFALVLICPFIALSQEINVLPKQKQDDVDRYREL
                     VERYNQADNPQQAAFYLNKIAFIYWENGNVNEAIKYFLETVPLNEKIGNYTDVKAVYS
                     NVALIYADMDRLDLTLEYFNKSLDARRKLNDKGELAAGLIDVAYIHTALNQNEKAVQL
                     LDEALALSKEVNNPRLILNCLRLLSQNYDKMGNTAKANEYAGMFAVYEQQLSTQQLKT
                     EYETKVVKSEADAERERMQRTQQALLMSLRELQAKSTQDSLSYMLLQRQDSLKKAEEQ
                     ARQRQTEIDLLNTDRKLKEAQISEQKAQSRIQRIIIFFTLVFLVVVIVSTIFIYRNYR
                     DKKKANELLNLQNVEIQKQRDQIQKQNENISKSINYAQGIQKALLPPQENLQAIFPES
                     FIFFRPRDVVSGDYYWFRELSTSPGSSQKNGKVAVSAIDCTGHGVPGAFLSMIGYNLL
                     DDIVERGVHKPGAILRDLNNGIRRALRQDETDNRDGMDMALCVVDFNNKVVEFSGAKN
                     PLVYVANNEVFRLRGDKESIGGGLGYMEREFTTHSIKIEGPTWFYQFSDGFIDQFGGP
                     DGRKFMIKNFTDLLAGISILPSGQQRDILKSTFKEWLGSKYPQVDDILVVGFKVDL"
     gene            2384..3247
                     /locus_tag="PK839_08560"
     CDS             2384..3247
                     /locus_tag="PK839_08560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72825.1"
                     /translation="MRKQVVAAFCVAGLAMASEKLIAQDFEVAPVKLMFDAEPATSQT
                     KTISVKNHSSRRVSYTVAVADFLPSNTGEKKTLPPNSTKRSCANWLNINPSFFDLNPG
                     EDIQVQVTMLVPGEEYGSTWCMLYIQPTTEQTSWTADKQLSTGITVTGRIGVQVYQSP
                     KSNQFSSVKISNLQEITQPGDKLRRFSASVENLGDKVTMCKIFLLASNMVTAEERQFA
                     AQNIEVFPKMSRNIELELPNDLVAGTYALAAIVDYGPKFPLEGTQIIIEVKGENRSIN
                     PLPPDTTSLSK"
     gene            3260..4417
                     /locus_tag="PK839_08565"
     CDS             3260..4417
                     /locus_tag="PK839_08565"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HOG72826.1"
                     /translation="MPGSIKFILAVCLSFMLAPAAAQKTIVFVEKTGQNGLLKEKYQG
                     VVSDGDTIRQGPYVYYSDNGILIQEGYYNAGKPDSIWLTYFPSGARMSLFRYKNGVKD
                     GEFIFWNQEGSVYQKGTYKNNLLHGEVTTYHRNGNVDTRSVYRNGRLNGLTEVFAEDG
                     KIKLRSEYLNDTLNGVWQTYYESGELNYSGSKSKGKYDNVLLAYHKNGSLQRAARYHD
                     GVLDGEVVSYYENGKPQVTSHFSMGKQTGEFVEYYPNGYVRERGMYKENQKEGMWMSF
                     YPNGQLHGKGNFKQGSLIGEWIFFHDNGNLKQRGSYCNGKACGLWQYFNPQGNLILCG
                     VYVDDLQNDIWTTFYPNGSVAGRILFVNNKPAGIRWVYQQNGSMEKRGGQL"
     gene            complement(4506..6482)
                     /gene="gyrB"
                     /locus_tag="PK839_08570"
     CDS             complement(4506..6482)
                     /gene="gyrB"
                     /locus_tag="PK839_08570"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HOG72827.1"
                     /translation="MSSEGLKGEELISNGYSADSIQVLEGLEAVRMRPSMYIGDTSIR
                     GLHHLVYEVVDNSIDEALAGFCNRIDVTINEDGSITVVDNGRGIPVDYHEKEKKSALE
                     VVLTVLHAGGKFDKGSYKVSGGLHGVGVSCVNALSIKLVAEVMRDGKLYRQEFSMGKP
                     VTSLEVVGESPSTGTIITFMPDSQIFTQSISFNFEIIASRLRELAFLNKGIRLTLTDK
                     RELVENGEAHYRHEEFYSVEGLKEFIKFLDGNRESLTEKIIYIESDKGDVPVEVAMQY
                     NTSFSENVHSYVNDINTVEGGTHLTGFRRALTRTLKKYADDSGMLSKLKFDINGDDFR
                     EGLTAVISVKVAEPQFEGQTKTKLGNPEVSLAVDQAVSEALANYLEENPRDARNIVQK
                     VILAAQARHAARKARELVQRKTVLSGSGLPGKLADCSDKDPLKTELFLVEGDSAGGTA
                     KQGRDRSFQAILPLRGKILNVEKAMQHKIFENEEIRNIFTALGVSIGTEEDSKALNLS
                     GLRYNKVIIMTDADVDGSHISTLIMTFFFRYMTDLINEGHLYIATPPLYLVKKGKEDR
                     YCWNEEERLKAVQELGKGRDSSVHVQRYKGLGEMNAEQLWITTMCPDTRFLRQVTIES
                     AAEADRIFSMLMGDEVPPRREFIEAHAKYANIDA"
     gene            6806..7600
                     /locus_tag="PK839_08575"
     CDS             6806..7600
                     /locus_tag="PK839_08575"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011203420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HOG72828.1"
                     /translation="MTQYLELLEHILKNGVQKTDRTGTGTISVFGYQMRFDLSRGFPL
                     LTTKKLHLKSIIYELLWFLRGDTNIKYLNQNGVTIWDEWANREGELGPIYGYQWRSWP
                     ASDGRHIDQIAQVVGSITQNPDSRRHLVCSWNVGDIGKMALPPCHVLFQFYVAGGKLS
                     CQLYQRSADVFLGVPFNIASYALLTTMVARVTGLEPGEFIHTLGDAHIYLNHVEQVKL
                     QLTRDPRPLPTMHINPQVKSIFDFSYDDFSLENYNPHPHIKGNIAV"
     gene            7727..7894
                     /locus_tag="PK839_08580"
     CDS             7727..7894
                     /locus_tag="PK839_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOG72829.1"
                     /translation="MAYKISDDCTACGTCIDECPVEAISEGDIYKINPDICTDCGSCA
                     DVCPVEAIHPE"
     gene            8020..8880
                     /locus_tag="PK839_08585"
     CDS             8020..8880
                     /locus_tag="PK839_08585"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOG72830.1"
                     /translation="MQIKKRTAQPFRKPLMLAVLAMSATLSFGQPFGVLTYNIRLDTE
                     QDGVNRWDNRKESMVELVNRYSPAIFGVQEALYHQVCYLDSALKKYTYIGVGRDDGKK
                     AGEFSAIFFDNTRLRVIAHSTFWLSEHPDSVSLGWDAACRRVCTYGLFEDMLSHKKFW
                     VFNTHFDHVGKVARKNSAELIVNRINELNGDNLPVIAMGDFNSNPADEPVKILASTLA
                     DGLTCSQKPLNGPVGTFNGFNPEYLPDERIDYIFVLKVRVLTYSHLTDRRENGLLISD
                     HLPVLAEVEI"
     gene            complement(8979..10070)
                     /locus_tag="PK839_08590"
     CDS             complement(8979..10070)
                     /locus_tag="PK839_08590"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOG72831.1"
                     /translation="MSTPRQLNILVCPLGWGLGHASRVVPIVRLLLESGHRVVVAADA
                     NSLALVKSSCPYLETKFFPSYKVQFKRGSSQLLPMLWVAIRLPFINLREHRKLKKLVL
                     ENHIDLVISDNRYGLWLRGIMSVMVTHQLRVIPPRPFKFLTKASEILIRHWLKRFNHV
                     WIPDNEGAGSIAGVLSSPNGLTNIQYIGILSRFSGIAPASNFKTWEMVVMVSGPEPQR
                     ALLVELAANLAHRYSIGCLVVEGKPQNGSELRVVDGIWYAGHLPDEQLAFVLRSCRYL
                     LVRGGYSTIMDLVTLGISGLLIPTPGQTEQEYLAHYLSVKGLFRSVPQQCLPQLDLKE
                     AERRSIPLHSEYSGSQRLKSCVDGVVFRL"
     gene            complement(10074..10847)
                     /locus_tag="PK839_08595"
     CDS             complement(10074..10847)
                     /locus_tag="PK839_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOG72832.1"
                     /translation="MSTFLFDSIVFGPVRSRRLGASLGMNLLPPDRKVCSFNCVYCEC
                     GWTDSIDSDKLPSRGQVQAALEYKLKKMISGGEGLDVITFAGNGEPTLHPDFSGIIDD
                     TIELRNTYFPNADIAVLSNATMLHRPDVVAALKRVDKNILKLDSAFEATINALNQPLI
                     RFDLKNLVDRLMAFEGKLIIQSLFVRGSINGIPVDNTTPAEISAWLKLVDQIRPSEVM
                     IYTIARDTPANDLKKLSEEELNGIAQKVEMLGIATQVSA"
     gene            complement(11139..11690)
                     /locus_tag="PK839_08600"
     CDS             complement(11139..11690)
                     /locus_tag="PK839_08600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72833.1"
                     /translation="MFVSGSINLSTSMPKTTTGSVTTEGARYTYFRVAPAVGYFLNDK
                     LAVGAGVSYSLQKQKQNDVANLTSMFGITPFVRYYAVSLGDVNIFAQGNVGIEFGANK
                     TKNDGNTTDGPKYLNFGISVMPAISYSLGEKLAIEAFLGSVYYSFNREKDEIAGETHT
                     LKTNGFGLNFDMSSISLGVIYKF"
     gene            12191..14392
                     /locus_tag="PK839_08605"
     CDS             12191..14392
                     /locus_tag="PK839_08605"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOG72834.1"
                     /translation="MERKIYPVEGMSCASCAMTVEKTIKNQPGVEDAGVNFANSTVWV
                     TFDPKAVKPEDLRDSVRSAGYDLIVDAVDVVKVGEEAKQKYADELKLRFWMSAAFSVP
                     LLVLGMFFMDWKLTPWLSAIMAAPVVFWFGRQFFTNGWKQARHFRANMDTLVALSTSI
                     AYLFSLFNTIYPEYWTSRGLQGHIYYESASIIVAFILLGKWLEEKAKNQTTSSIKKIM
                     GLQPDMAWLVEGESLSEVPLSRIRKGDVVLVKPGSRVPVDGRVVDGQSVVDESTISGE
                     SIPVVKKVGDKAWAGTMNQRGSFKLIAEQVGSESVLGRIIATVESAQNSKAPVQHLVD
                     KVAGIFVPAVMLVALLTLITWLVLDPANGFSHGLISMVTVLAIACPCALGLATPTAII
                     VGVGRAAEKNILIRDAESLETAHQVNYIVLDKTGTLSEGVPSVDRIEWFTHETTYPGI
                     LKFIEQNSGHPLADAIVRHFETADSSGFVADSYESIAGKGVKTTVKGKKYLVGNPDFL
                     RQEGVMFTDTIESSLSEILNNAGSVVGFAENNLLLCLIRVSDRIKPSSREAIKRLKAR
                     NIEVAMLTGDNRANAEFMARELEIAHYAGQQLPSDKADFIKTLKKQGKVVAMVGDGVN
                     DSEALSVADVGIAMGKGSDIAIDVAQVTILNSDLNMLVTLLDISGKTIRTIRQNLFWA
                     FIYNIIGIPIAAGLLYPFYGITLDPMFAGMAMALSSVSVVTNSLRLRTARI"
BASE COUNT         3987 a         3370 c         3326 g         3825 t
ORIGIN      
        1 aacgtttttc tcctttacga gcactgcgga gaatattttt cccccgttgg ggctgaaatt
       61 tgaaagctga agagcttaaa ttgaaaaaat aatctacatg ctacaaatca accttgaacc
      121 caactaccag aatatcatca acctgaggat actttgaccc cagccattct ttaaacgtac
      181 tcttcagaat atcgcgctgc tgaccggagg gaaggatgga aatgcccgcc agcaggtcgg
      241 taaagttttt aatcataaac ttacgaccat caggaccccc aaactggtca atgaatccat
      301 ccgaaaattg gtagaaccag gttggcccct cgattttgat ggaatgggtg gtaaactccc
      361 tctccatgta gcccaaacca ccaccaatgg actccttgtc gccccgaagc ctgaacacct
      421 cgttgtttgc cacataaaca agcgggtttt ttgctcccga aaactcaact accttgttgt
      481 taaaatctac cacacacaag gccatgtcca taccatcgcg gttgtctgtt tcatcctgtc
      541 gcaaggcacg cctgataccg ttgttcaggt cacgaagaat agccccaggc ttgtgaaccc
      601 cacgctcaac aatatcgtcg agaaggttgt agccaatcat ggaaagaaaa gcacccggta
      661 caccgtgccc ggtgcagtca atagccgaaa cggctacttt cccatttttc tgtgaagagc
      721 cgggtgaggt ggatagctcc ctgaaccagt agtagtcgcc gctcaccaca tcgcggggac
      781 ggaaaaatat gaacgactcg gggaaaatcg cctgcaggtt ttcctgtggc ggtaacaggg
      841 ctttctggat tccctgcgca tagttgatgc ttttgctaat gttctcgttc tgcttctgaa
      901 tctgatcgcg ctgtttctga atttcaacat tctgcaggtt gagaagctcg ttggcctttt
      961 tcttgtcccg gtaattcctg tagataaaga ttgtggaaac aatcaccacg acaagaaaga
     1021 ccaatgtaaa aaatatgatg attcgctgga tgcgcgactg agctttctgc tcgctaatct
     1081 gagcttcttt aagcttgcgg tcggtgttca gcagatctat ctcggtttgg cgctggcgtg
     1141 cttgctcctc agctttcttc aggctgtcct gacgctgtaa aagcatatag ctaagtgaat
     1201 cctgggttga cttggcctga agctcacgca agctcatgag gagcgcctgc tgggttcgct
     1261 gcattctctc acgttcggca tcggcttcgg acttaaccac cttggtttca tactcggttt
     1321 taagctgttg ggtggatagc tgctgctcat acacggcaaa cattccggca tactcgtttg
     1381 ccttagcggt attccccatc ttatcgtagt tctgcgaaag cagcctcaaa cagttcagaa
     1441 ttaaccgggg gttgttaact tccttcgaaa gggccaaggc ttcatcaagc agctgcacgg
     1501 ccttttcgtt ttgattcaag gctgtgtgga tatatgcaac atctatcaaa cctgcagcta
     1561 gttcgccctt atcgttaagc tttcgccgag catccaggct cttgttgaag tactccagag
     1621 tcaggtcgag ccggtccatg tcggcgtata taagagcaac gttgctgtaa accgccttta
     1681 catcggtata gttgccaatt ttttcgttca ggggaaccgt ttcgagaaaa tactttatag
     1741 cctcgtttac atttccgttc tcccagtaaa taaaggctat cttattaagg taaaaagcag
     1801 cctgctgagg attatcggct tggttgtacc gctcaaccag ctccctatac ctgtcaacgt
     1861 catcctgctt ttgcttgggg agcacgttaa tttcctgcga aagcgctata aagggacata
     1921 tcaaaaccag tgcaaacacc agagttgacc ccaccaaaat ccttttcaat ttatacccag
     1981 ttgattttaa atcaaacgta caatattttt tcaatacgat acaaaataaa cctacttttt
     2041 ttataagaat ggaaattatg atgacaagca tgtgtatgta gtctctaaag gtaaaataga
     2101 gagctatgaa gctccacctt acaaggatta ccagccatgt tttaagcaag aggctagcta
     2161 caatcaccat aatgcaggga tacggttaaa tcgccatcga aacaacccga gcaagaaaaa
     2221 taaaataata tctcaacacc ttagtcattg attaatcatc atttacatat caaattttgc
     2281 ctgctatcaa ataaattcat ttacctttgc agaattgatt agtagttctt caaaaaagca
     2341 tgatatttaa taattttgtt agcttaagca ttgaccaaca accatgagaa aacaagttgt
     2401 agcagcgttt tgtgtagccg gattagcgat ggcatcagaa aagttgattg cacaggattt
     2461 tgaggtagcc ccggtaaaat tgatgtttga tgccgagcct gctaccagcc aaaccaaaac
     2521 aatcagcgtt aagaaccaca gcagcagaag ggtttcatac acagttgcag tggccgattt
     2581 tttgccatca aacacgggtg aaaaaaaaac actacctccc aactccacca agcgctcctg
     2641 cgccaactgg cttaacatca acccgtcgtt cttcgacctt aatccaggag aagatattca
     2701 agtacaggta accatgctgg ttcctggcga agagtatggc tcaacatggt gtatgctata
     2761 catccagcct actacagagc aaacatcatg gactgccgac aagcagctaa gcacgggtat
     2821 caccgtaacg ggtagaattg gtgtccaggt ttaccaatcg cctaagtcga accagttcag
     2881 ctcggtaaaa attagcaact tacaggagat aacccaacca ggcgataagc tgcgccgatt
     2941 ttctgcttca gtcgagaacc tgggcgacaa ggtaacgatg tgtaagattt ttctgctggc
     3001 ttcgaacatg gtaactgccg aagagagaca gtttgccgct cagaatattg aagtattccc
     3061 aaagatgagc cgtaacatag agctggagct tcccaacgac ctggtggccg gaacatacgc
     3121 cctggcagca attgttgatt acggcccaaa attccccctt gagggaactc aaattataat
     3181 tgaggtgaag ggtgaaaatc gaagtattaa cccgttaccc cccgacacca cgtcgctttc
     3241 aaaataaaaa atcaccgaat tgcctggcag cattaaattt attttggcag tttgcctttc
     3301 cttcatgctg gctccagcag cagcacagaa aactatagtg tttgttgaaa aaacagggca
     3361 aaatgggctg cttaaagaaa aataccaggg ggttgtaagt gatggtgata ccattcgtca
     3421 gggaccttac gtgtactata gcgataacgg catactaata caggagggtt actacaacgc
     3481 cggtaagcct gacagcatct ggttaacata ctttccgtcg ggagcaagga tgagcctttt
     3541 ccgatataaa aacggcgtta aggatggtga gtttatcttt tggaaccagg aaggaagcgt
     3601 ttaccagaag gggacttaca aaaacaacct acttcacgga gaggttacca cctaccatag
     3661 gaatggcaat gttgacaccc ggtcggtgta ccgcaatggc aggctaaacg gattaaccga
     3721 ggtatttgcc gaagatggaa aaataaagct tcgctcggag tacctaaacg ataccctaaa
     3781 cggagtgtgg cagacctact atgaatcggg agagctaaac tatagcggaa gcaaaagtaa
     3841 aggtaagtac gacaacgtgc tgctggctta ccataaaaat ggatcgcttc agcgcgctgc
     3901 acgataccac gatggggttc tggacggcga ggtagtttcg tactatgaaa acggtaagcc
     3961 tcaggtaacc tcgcacttct caatgggaaa gcaaacaggc gagtttgttg agtactaccc
     4021 caacggctat gttagggaaa ggggaatgta caaagagaac caaaaggagg gcatgtggat
     4081 gagcttttac cctaacgggc agcttcatgg aaagggaaat tttaaacagg gtagccttat
     4141 cggcgaatgg atattttttc atgataacgg aaacctcaag caacgtggaa gctactgcaa
     4201 tggtaaggct tgtggcttat ggcagtactt caaccctcaa ggaaatttaa ttttgtgcgg
     4261 agtttacgtc gatgatttac aaaatgacat ttggactacc ttttacccaa atggttcggt
     4321 agctggcaga atactttttg taaacaacaa acctgctgga ataagatggg tttaccagca
     4381 aaatggatca atggagaaga gaggcggaca gctttaggtt cttcaactca accccacaat
     4441 aacaaataag aggctgaatg tcagcctctt atttgttata taacgtttat actccaacag
     4501 gcattttagg catcaatgtt ggcgtacttg gcatgtgctt caataaactc gcgcctaggc
     4561 ggaacctcat cgcccatcag catggagaat atcctgtcgg cctcagctgc actttcgatg
     4621 gtaacctggc ggaggaatcg ggtatcggga cacatggtgg taatccaaag ctgttcggca
     4681 ttcatctcac caagcccttt gtagcgctga acgtgaacag agctatcacg tcccttcccg
     4741 agctcctgaa ctgcctttag cctctcctcc tcattccagc agtagcgatc ctccttacct
     4801 tttttaacca ggtaaagcgg gggagttgca atgtagaggt gtccctcgtt aataagatcg
     4861 gtcatgtagc ggaagaagaa agtcatgata agtgttgaaa tgtggcttcc gtccacgtcg
     4921 gcatcagtca taatgattac tttattgtaa cgaaggcccg acaggtttaa ggccttgcta
     4981 tcctcttctg tgccaatact tacaccgagt gcagtaaaaa tattccgaat ttcctcgttc
     5041 tcaaatattt tgtgctgcat ggccttttcg acgttcaaaa tttttcccct caagggcagg
     5101 atggcctgga agctcctgtc gcgtccctgc ttggcagtgc cgccagccga atcaccctct
     5161 accagaaaaa gttctgtttt caggggatcc ttatcggagc aatcggctag cttacctgga
     5221 agtcctgagc cggaaagcac ggtttttctt tgcaccagtt cccttgcctt gcgggcagca
     5281 tgtcgtgcct gtgcggcaag tatgaccttt tgaacaatgt tgcgggcgtc gcgggggttt
     5341 tcttccaggt agtttgccag cgcttcgctt actgcctgat caacagccag gctaacctcg
     5401 gggttgccaa gcttggtttt ggtttgaccc tcaaactggg gttcggccac cttaacggat
     5461 ataacagccg ttaatccctc ccggaagtca tcaccattga tgtcgaattt cagcttggaa
     5521 agcatccctg aatcatcggc atactttttc agcgtccggg taagcgctct tcgaaaacca
     5581 gttaggtgtg tgcccccctc aacggtattg atatcgttta cgtaggagtg gacattttcg
     5641 gaaaacgagg tgttgtactg catggccacc tcaaccggaa catccccttt atcggactcg
     5701 atgtaaatga ttttctcggt caggctctcc cggttaccat cgaggaactt gataaactcc
     5761 tttaacccct caaccgagta aaattcctcg tggcggtagt gggcttcgcc gttttcaacc
     5821 aattctcgct tgtcagtgag tgttaggcga atgcccttat tgaggaatgc caactcgcgt
     5881 aaacgcgagg caataatttc aaagttgaag ctaatggatt gggtgaatat ctgcgaatcg
     5941 ggcatgaagg taatgatggt gcctgtgctg ggtgactctc caaccacctc gagcgaagta
     6001 acaggctttc ccattgaaaa ttcctgacgg tatagctttc catcgcgcat aacctcggca
     6061 acaagcttta tggagagggc gttaacacaa gaaactccaa ccccatgcag ccctcctgat
     6121 actttatagc tgcccttgtc aaactttccg ccagcatgta gaacggttag caccacctca
     6181 agtgccgatt tcttctcctt ttcatggtag tcaaccggaa ttcccctccc attatccact
     6241 acggtgattg atccatcctc gttgatggta acatcaatcc tattgcagaa accggctagg
     6301 gcttcgtcaa tggaattatc aactacctcg taaacaaggt ggtgtaatcc cctgatgctg
     6361 gtgtcgccaa tgtacattga tggtctcatg cgaacggcct ccagcccctc aagaacctgt
     6421 atgctatcgg cgctataacc gttggaaatc aactcttctc ccttcaaacc ttcgctgctc
     6481 atactactta taaatatttg gaatcagttg cacactttag tattgtaatt aataagctaa
     6541 tttttaaaag ccatcaaaag tagtaaaaat tttggttaaa cagcacttca aaaattactt
     6601 tccaataatc atatagtgct attatacaga cacatacgta tttgttgata aaatatttca
     6661 aaaagtatgc ccgggttaca gcagctgttt gttgttctgt ttttaaaaga agaaaagctt
     6721 gagagaaaaa gctaacttcg cagcaaaacc tgaggtagcc catttggcat agttgaattt
     6781 tattacctca aatagaactt acaccatgac ccaatatctt gaactactag aacatatatt
     6841 gaagaacgga gtgcagaaga ccgacaggac gggcacagga acaatcagtg tttttgggta
     6901 ccagatgcgc tttgatctta gcagagggtt tcctctgctc accacaaaaa aactacacct
     6961 gaagtcgatc atttacgagc tgctgtggtt tttaagagga gataccaata ttaagtatct
     7021 aaaccaaaac ggcgtaacca tttgggacga gtgggccaac cgcgaagggg agttggggcc
     7081 aatatacggc taccagtggc gctcgtggcc agcttcagat ggccggcata ttgaccagat
     7141 agcacaagtt gtcggctcaa taacccaaaa tcccgattcg cgcaggcatt tggtttgctc
     7201 gtggaatgta ggcgatatag gtaaaatggc acttcctcct tgccatgtgc tattccaatt
     7261 ctacgttgct ggcggtaagc tctcctgcca gctttaccag cgcagcgccg atgtttttct
     7321 tggcgttccc ttcaacattg cttcgtatgc tctcctaacc actatggtag cccgtgttac
     7381 cggattagaa cccggtgaat ttatccatac gctgggtgat gcccacatct acctcaatca
     7441 tgttgaacag gttaagctgc agctgacgcg cgacccgcgc ccgttaccca ccatgcacat
     7501 taacccgcag gtgaaatcta ttttcgattt cagctatgat gactttagcc tggagaacta
     7561 caacccgcat ccccatatca aggggaatat cgccgtttag tgatactcga ctgtgaatct
     7621 cgaaatctct cctacaagaa ttaattaaaa tttcacatca tattttttgg taatacatta
     7681 aaaagctatt tttgtgatga attcagttta actaaaactt gcagaaatgg catacaaaat
     7741 ttccgacgat tgcaccgctt gtggtacttg cattgatgaa tgtcctgttg aggcaatatc
     7801 cgagggcgat atctataaga ttaaccccga tatttgcacc gactgtggtt catgtgccga
     7861 tgtttgcccg gtagaggcca ttcatcccga gtaattacag cagtaatttt atcaaaatcc
     7921 gcaaatcatg cggattttgt tttatatatg aataccccct gattggtagc atgacgaact
     7981 gaacaaacca tgctcctttg agctaaaacc gcttcaacaa tgcaaatcaa aaaaagaacc
     8041 gcacaacctt ttcgaaaacc tttaatgctg gcagtacttg caatgagcgc cacgctaagc
     8101 tttggtcaac cattcggcgt gctaacctac aacattcgcc tcgataccga gcaggatggg
     8161 gtaaatcgat gggataatag gaaagaatca atggtcgagc tagtcaaccg ttacagcccc
     8221 gccatatttg gagttcagga agcgctgtac catcaggtct gctaccttga tagtgcctta
     8281 aaaaaataca cctacattgg cgttggcaga gacgatggga agaaggccgg cgaattcagc
     8341 gctattttct tcgacaacac caggcttagg gtgattgccc actccacctt ctggctatcg
     8401 gagcaccccg atagcgtttc attaggctgg gacgcagcct gcagaagagt ttgcacatac
     8461 gggctttttg aggatatgct ttcccataaa aaattttggg ttttcaacac ccacttcgac
     8521 catgtgggta aggttgcccg taaaaattcc gcagagttga tagtcaacag gataaatgag
     8581 ctgaatggcg ataatttgcc ggtaatagca atgggtgact tcaacagcaa tccggctgac
     8641 gaaccggtga agatattagc ctcaacgctt gccgatgggt taacttgctc tcaaaagccg
     8701 ttgaatggac cggttggtac ctttaacggg tttaaccctg aatacctacc tgatgaaagg
     8761 attgactata tatttgttct gaaagtgcga gtgctcacct acagccatct taccgaccga
     8821 agggaaaatg gtcttttaat ctccgaccat cttcctgttc ttgctgaggt ggaaatatga
     8881 ataccttcaa tttaagattt tattgaattt actagagttg tcttagccta aatcatggca
     8941 ttgtttcggc ctgttatttt tttttggcaa aagtgattct agagtctgaa aacaacccca
     9001 tcaacgcagc tctttaacct ttggctacca gagtattctg aatgcagggg tatgctccgt
     9061 cgttctgctt ctttcaagtc gagttgaggc aggcattgct gcggaaccga ccggaataga
     9121 ccctttaccg acaggtagtg cgccaggtat tcctgctcgg tttggcctgg agtgggaata
     9181 agcaatcctg aaatgcctag ggtaacaaga tccattatgg tgctataacc acccctgacc
     9241 agcaggtacc tacagctacg taacacgaat gccagctgct cgtcgggtaa atgaccggca
     9301 taccatattc cgtcaaccac tctaagttca ctaccatttt gaggtttccc ctccaccacc
     9361 aggcaaccaa tggagtaacg gtgggccagg ttggctgcca gctcaaccag aagggcacgc
     9421 tggggctcgg gacccgaaac cataactacc atttcccagg ttttaaaatt ggatgctggc
     9481 gctatccctg aaaaacgtga tagtattccg atgtattgaa tgttggtcaa tccgttaggg
     9541 ctggacagca ctccggcaat actgccagca ccctcattat cgggaatcca aacatggttg
     9601 aagcgtttca accaatgtct tattagtatt tccgacgctt tagtaaggaa tttgaaaggt
     9661 ctgggcggaa ttaccctaag ctggtgggtt accatcaccg acataattcc acgcagccac
     9721 aacccgtagc ggttgtcgga tatcaccagg tcaatgtggt tttccaaaac cagctttttc
     9781 agctttctat gttcccttag gttgatgaag ggaagcctga tggcaaccca aagcataggc
     9841 agaagctggc tggagccccg cttaaattgt accttgtacg acggaaaaaa tttggtttct
     9901 aagtaagggc atgagctttt taccagagcc agcgaattcg catcggctgc aaccaccacc
     9961 cgatgtccgc tttccagcag taagcgaaca atagggacaa ccctgcttgc atggccgagc
    10021 ccccatccaa gagggcatac taaaatgttc agctgccgag gggtgctcat tatttaggct
    10081 gaaacctgcg ttgcaatacc caacatctcc accttttggg caatgccgtt gagctcctcc
    10141 tcagatagct ttttgaggtc attggccggg gtatcgcggg caatggtgta aatcatcacc
    10201 tcgctaggtc gaatctgatc aaccagcttg agccatgccg agatttcggc aggtgtggtg
    10261 ttgtcaaccg gtattccgtt gatgctgccc cttacgaata acgactggat tatcaatttc
    10321 ccttcaaaag ccatcaagcg gtcaacaaga ttttttaaat caaaacggat gaggggttgg
    10381 ttgagagcgt tgatggttgc ctcgaaggca gaatcgagtt ttaaaatgtt cttatccact
    10441 cttttaaggg ctgcaaccac atcgggacga tgcagcatgg tggcattaga gagtacggca
    10501 atgtcggcat tagggaagta tgtattcctc aactcaatag tatcgtctat aattccggaa
    10561 aaatcaggat gaagtgtagg ctcaccattg ccagcaaagg taattacatc aaggccttca
    10621 cccccgctaa tcattttctt tagcttatac tcaagggctg cctgaacctg cccccgtgaa
    10681 gggagcttat ccgaatcaat ggagtcggtc cagccacact cgcagtacac gcagttgaac
    10741 gagcaaactt ttcggtcggg cggaagcagg ttcattccca gcgaggcacc aagcctccgg
    10801 ctacgcaccg ggccaaacac aatgctatcg aacagaaagg ttgacatagc tgctgatttt
    10861 tcgcaaagat acccttttgg agcaagatgg tgtgcccgtg ttcaactttt ggaaattatg
    10921 aacaattcaa agcttgacat tctattaaat actaatgtgt tgggtatgct gcatacacga
    10981 tactaaagca cgaaccagct gattcatcaa cttctgcata acaatctata aaaattatcg
    11041 gcggcaaccc ccaactttaa atcgtttggg gttgccgcca gatgaataaa acctccagtg
    11101 ggattaactc ctaaaggtgg aaatcccctt aatgcttatt aaaacttgta aataacgcct
    11161 agcgatatgc tagacatgtc gaaattcaag ccaaaaccgt tagtttttaa agtgtgagtt
    11221 tctccggcaa tctcatcctt ttccctgtta aaagaatagt acacactacc caggaaggct
    11281 tcaatagcca gcttttcgcc caagctgtag gagatggcag gcataacgct aataccaaaa
    11341 tttaggtatt taggcccatc agtagtattc ccatcattct tcgttttgtt ggcgccaaac
    11401 tctattccga cattaccttg tgcaaaaata ttcacatcac ccagacttac agcgtagtac
    11461 ctgacaaacg gagtaattcc aaacatgctg gtaagattgg ccacatcgtt ttgcttttgc
    11521 ttttgaaggc tatagctaac acctgcacct actgccaact tgtcgtttaa aaaatacccg
    11581 actgcagggg caacacgaaa ataggtgtat cgtgctccct cggttgtaac cgacccggtt
    11641 gtagtttttg gcattgaggt tgaaaggttg atgctaccgc taacaaacat ttgcgcattt
    11701 actacgcctg cgatcgtggt taatgcaatt aatacaaaaa aactcctgat gattttcatt
    11761 acgattcata tttaatgtta ggcctactct taggtttttc ggcatacacc tataattaaa
    11821 ctcagcaatt gacgtattgt tcaatttcga tattatgacg gttatagggt ttaagccatc
    11881 cctaccatgt tatcgccgat agctactttt gcctagaaaa attatttcaa cttaagtaaa
    11941 tttgcaacag caaaaattgc gccagctgcc attaggctag aggctgaatc cccctttatg
    12001 tatcaagttt gctctgctga ttcctatgcc ccagagtgtt atgcgaattg cttcccacaa
    12061 gtagtatgca tttggtaagc cattagctgg tttgatggaa agaaaataca cggtttaaga
    12121 ataagttttt atatcatgca aaacgttaaa cacaaatgga tttgacctgt ttagtaagca
    12181 aaaaatggct gtggaaagaa aaatataccc ggtagaagga atgtcgtgcg ccagctgcgc
    12241 catgaccgtg gaaaaaacca taaagaacca accgggggtg gaggatgcgg gcgttaactt
    12301 tgccaacagc acggtttggg ttacttttga tcctaaggct gttaaacctg aagatttaag
    12361 ggattctgtt cgttcggctg gctacgatct catagttgat gccgttgacg tggtaaaagt
    12421 tggcgaggaa gccaagcaga aatacgccga tgagctaaaa ctgcgcttct ggatgtcagc
    12481 agcattctca gtgcctcttc tcgtgttggg aatgttcttt atggattgga agctaacccc
    12541 gtggctgtcg gccattatgg ctgctccggt ggtattttgg tttggcaggc aattctttac
    12601 taatgggtgg aagcaggccc ggcatttcag ggcaaacatg gataccctgg ttgcgctaag
    12661 cacttcaatt gcctacctct ttagtctttt taacactatt tacccggagt attggacaag
    12721 tcgaggcctt caggggcata tctactatga atcggcttct ataatagtag cctttatact
    12781 gctaggaaag tggcttgaag agaaagctaa gaatcaaacc acctcatcaa taaaaaaaat
    12841 catgggcttg caacccgata tggcctggtt ggttgaggga gagagcctga gcgaggtacc
    12901 gctatcccgt attaggaagg gcgatgtagt gctggtgaag cctggtagta gagtccctgt
    12961 tgatggaaga gttgtggatg gccaaagcgt ggttgatgag agcaccattt cgggcgagtc
    13021 aatacctgtt gtcaaaaaag tgggtgataa agcatgggcc ggaacaatga accaacgggg
    13081 cagctttaag ttgatagccg aacaggttgg gtcggaatcg gtgctgggta gaatcatcgc
    13141 tacagttgaa agcgcacaga acagcaaagc tccggtgcag catctggttg acaaggtggc
    13201 aggaatattt gttccagcgg taatgctggt tgccctactt acccttatta cctggcttgt
    13261 gctagatccg gctaacggct tctcacatgg gctaatctcc atggttaccg tgttggccat
    13321 agcttgtccg tgcgccttgg gtctggcaac gccaacagcc attattgttg gagttggacg
    13381 ggcagcagaa aaaaacatcc tgattcggga tgccgaaagc ctcgaaacag cccaccaggt
    13441 aaactacatt gttctcgata aaacagggac gctgagcgaa ggcgtgccca gcgtggatag
    13501 aatcgaatgg tttacccatg aaaccaccta tccgggtatt ttaaaattta ttgagcagaa
    13561 ttcgggtcat ccactggccg atgctattgt tcggcatttc gaaacagccg actcctcggg
    13621 atttgtggcc gatagctacg agtccattgc cggtaagggg gtaaagacaa cagtaaaggg
    13681 aaaaaagtat ttggttggca atccagactt tcttaggcag gaaggagtga tgtttaccga
    13741 tacaattgaa agcagccttt cggagatttt aaacaacgcc ggatcggttg tagggtttgc
    13801 cgaaaataac cttcttctgt gcctgattcg ggtatcggac aggataaagc cctcctccag
    13861 ggaagccata aaaagattaa aggccagaaa catagaggtt gccatgctca caggcgacaa
    13921 ccgtgcaaat gcggaattta tggcacggga actcgagatt gcccactatg cagggcagca
    13981 gcttccgtcg gataaggctg atttcatcaa aacccttaag aaacagggaa aagtggttgc
    14041 catggtgggt gacggcgtta acgatagcga ggcgctaagt gtagctgacg taggaatagc
    14101 catgggcaaa ggctctgaca ttgccattga cgtggctcag gttactatcc ttaactccga
    14161 tttaaacatg ctggtaacgc tgctcgatat ttcaggcaaa actatcagga caatccgcca
    14221 gaaccttttc tgggcattta tatacaacat cattggtatt ccaattgcag cgggacttct
    14281 ttacccattc tacggaataa ccctagaccc aatgtttgca ggtatggcca tggctctcag
    14341 ctcggtgtcg gttgtaacga acagccttcg gctgagaacc gccaggattt gaaaaaggac
    14401 aaagtgagtt tgtacggcta atgactgatt caatataaaa aggataaagg ttaaaaaacc
    14461 tgtccgccta ccggcggaac ttgtccggct cctgacggat tccagaac
//
LOCUS       DAOSHM010000059        14246 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_3530_length_14246_cov_22.685434, whole genome shotgun
            sequence.
ACCESSION   DAOSHM010000059 DAOSHM010000000
VERSION     DAOSHM010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 14246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14246
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3530_length_14246_cov_22.685434"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(77..1393)
                     /gene="ftsA"
                     /locus_tag="PK839_08610"
     CDS             complement(77..1393)
                     /gene="ftsA"
                     /locus_tag="PK839_08610"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HOG72835.1"
                     /translation="MAHSNDYVVAIDLGTTKVVTMVGKMNSNGKLQVVGLSTAESTGI
                     KRGMIQNIEETVKAIQKTVDDVKKQVGLEFHSAYVGIAGQHIRSIKNSNYLNFDTDDR
                     EISQDDIDRLVKDMNLTPVDAGETILHVLPLEFFIDKEPVDNNRPVGMMGRRLDANFH
                     IVIGKTSSAKHIEKCVNRVGLSVADLILEPLASAEAVLTDDEREAGVALIDIGGGTTD
                     LAIYYEGNIRHTAVIPFGGNVITQDIKEGCSILLKQADQLKEIYGIALSDIAPDNKII
                     TIPGISGREPKEISVKNLAGIIQARMEEILDFVLYEINLSGYAEKLSAGVVITGGGAL
                     LKHLSHLIKFKSGYDVRLGFPSEKLSSDSLEKYNHPQLSTALGLALIGLERQTATPVQ
                     DDAKQQNATEPSVDKEFSEVHEKKGSKFNAIKQMISGLFEETDSEM"
     gene            complement(1398..2204)
                     /locus_tag="PK839_08615"
     CDS             complement(1398..2204)
                     /locus_tag="PK839_08615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72836.1"
                     /translation="MNRRTRNILIILGWLLLGSYLVYSLLFTAERAKKVLCRGIRVEV
                     VDSAQYQFVKPGTIRKMILSSTISPIGKPLNSINTHKLEKNISELVAVRDVQVYKSMD
                     GILRITVKQRRPIVRIFNARNQSYYIDDEGLVMPVSSSYAAYVLIASGNIYEPFRVKP
                     NIDITRWNDSLTNGSKPLVCQIFDFARYINHDAFWRAQINQIYVINARNVELIPVVGP
                     HVIAMGSLEGYDRKLKKLKLFYENALPTEGWNKYSRINLMYSNQIVCTKR"
     gene            complement(2207..3586)
                     /gene="murC"
                     /locus_tag="PK839_08620"
     CDS             complement(2207..3586)
                     /gene="murC"
                     /locus_tag="PK839_08620"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HOG72837.1"
                     /translation="MSMEQYLGVYFLGIGGIGMSALARYFALKGRVVAGYDRTPSAIT
                     SQLAEMGIGIHFTDDAELIPSEFRNPATTLVVYTPAVPETLAELAYFRRGGFQLLKRA
                     QVLGMLSLGYQTLAVAGTHGKTTTSTLLAYLLGSSSVGCDAFLGGISKNFGSNLVTAD
                     KGRKLLVAEADEYDRSFLNLHPQLAVITSVDPDHLDIYGDYERVVEAFNQFAGQVKPN
                     GTLIAKSGLGIAPIPERGVTRLTYGLSSDADFYPVNLSLNEGCYGFSLETPNGTIDDL
                     KLGVPGLFNLENSIAAAAAAITCGISHDELRSGLLSFSGVSRRFDIRFKSSRTVYVDD
                     YAHHPREIEAMIESARQVYPNRKITGIFQPHLYSRTRDFAFDFARSLDKLDMAILLDI
                     YPAREQPIEGITSQTIADKMNNPNRLLLDKADLLEWLRKNDVDVLLTMGAGDIDRLAP
                     EIVKIFEAK"
     gene            complement(3668..4795)
                     /gene="murG"
                     /locus_tag="PK839_08625"
     CDS             complement(3668..4795)
                     /gene="murG"
                     /locus_tag="PK839_08625"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008649233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HOG72838.1"
                     /translation="MEKNQLKVIISGGGTGGHIFPAIAIANAVKELSPEAEILFVGAL
                     GRMEMERVPQAGYRIVGLPIIGIRRSLSPQNIMLVFKLIRSLRLSRKIIADFRPDVVV
                     GVGGYASGPILRAAQKRGIPTLIQEQNSFAGITNKMLAAGAHKICVAYDGMERYFPKE
                     KIIKTGNPVRQDIETLKVTREEAFRFFNLSPEQKVLLVLGGSLGAGTINQSLRHKLDA
                     IARHPEVAVIWQTGKFYYEAINQELAGYSGRNIRLFDFINRMDMAFAAADLVISRAGA
                     GTISELCLVGKPAILVPSPNVAEDHQTHNAEALVKRNAAVMVADSDAEPTLVETAFSL
                     ISNEEKLRELAQNILTMALPNSATLIAGEVLSLANANSKSL"
     gene            complement(4764..5933)
                     /locus_tag="PK839_08630"
     CDS             complement(4764..5933)
                     /locus_tag="PK839_08630"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HOG72839.1"
                     /translation="MGTFVDKYFKGDRAIWLVVILLSAFSLLVVYSSTGSLAFRYHGG
                     NTTYFLVKQLVFLVLALSIVFVTHLISYRVYYPLSSLLMLISIPLLLFTLVFGTRLNE
                     ASRWLEIPGLGITFQSSDVAKFALVMYMAKILSQKQKDIKDLKQGFLPLALPVILICG
                     LILPANFSTAALLGLTCWIMMYVGRVNLKYLLGFTGIGVALFAIFVVVALHFGDVGRV
                     HTWKNRIENFVSGDNPDGNYQVEQSKIAIANGGITGKGPGRSTQRNFLPHPYSDFIYA
                     IIIEEYGLFAGLFIISLYLILLYRSAVIVKKAKRTFPAFLAFGLALLLTMQAMVNMAV
                     AVNLIPVTGQPLPMISMGGTSLFFSSAAFGIILSVSRLQSKKELFDGEEPAESNN"
     gene            complement(5945..7279)
                     /gene="murD"
                     /locus_tag="PK839_08635"
     CDS             complement(5945..7279)
                     /gene="murD"
                     /locus_tag="PK839_08635"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HOG72840.1"
                     /translation="MKRIAILGGGESGAGAAVLAFKKGFDVFLSDSSTIKPRYREWLQ
                     RYSIPFEENGHTEALILNADEVIKSPGIPDKASIVARIREQGIPVISEIEFAGRYNTA
                     RTICITGSNGKTTTTSLIYHMMQKAGLNVGLAGNIGNSFAYQVAEDNFDYYVIELSSF
                     QLDGVYEFKPDIAILLNITPDHLDRYDYKMQNYVESKFRITRNLSEDDCFIFCSDDAV
                     TVEQLKRIILRAQQLPFSQRVKENQAAWLDDDNLLINYNNSDFSMSINDLSLKGKHNI
                     YNSMAAGIAGHVLNIRKDVIRQSLSDFQGVEHRLEPVLTIKGVRYINDSKATNVNSAW
                     YALESMDTPVVWIAGGVDKGNDYSELMDLVKLKVKAIVCLGVDNVKLHKAFGNVVPVM
                     VDVLSAEDAVRSAYELANPGDTVLLSPACASFDLFENYEDRGRKFKDAVRML"
     gene            complement(7427..8671)
                     /gene="mraY"
                     /locus_tag="PK839_08640"
     CDS             complement(7427..8671)
                     /gene="mraY"
                     /locus_tag="PK839_08640"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HOG72841.1"
                     /translation="MIYYLVQYIQKQFDFPGAGLFRYISFRSALAFILSLLFALFIGK
                     RIIRYLQRKQIGEEIRNLGLEGQLQKKGTPTMGGIIVIAATLISVLLFADLLNIYIII
                     MLVTTVWMGAVGFTDDYIKVFLKNKDGLSGKFKILGQVLLGLFVGLTMWLSPDIMIRD
                     HVRPNIQSEVVVTSEYSGSEPVSVSKGKKTTQTTIPFFKNNEFDYAYLNPFKGKWRDA
                     ITWTIFIVVVIFIITAVSNGTNLTDGLDGLATGVSAMNATTLGVFAYLSGNVIYSEYL
                     NIMYIPHISELVVFMSALIGALIGFLWYNSYPAQVFMGDTGSLTIGGIIAVYAIVVRK
                     ELLLPILCGIFLVEALSVLIQVSYFKVTRKIYGEGRRVFLMSPLHHHYQKKGYAEPKI
                     VTRFWIISMLLAVITVVTLKIR"
     gene            complement(8668..10134)
                     /locus_tag="PK839_08645"
     CDS             complement(8668..10134)
                     /locus_tag="PK839_08645"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015532166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HOG72842.1"
                     /translation="MAVGLTKILPKALVNEVVGNPDIEVEQLTFNSRVAGANSCFFAI
                     RGTQADGHRFIPQAVENGASAIVCEAMPVSFAPNVTYVRVSDSSEALGLMASAFYGNP
                     SSNLKLVGVTGTNGKTTTATLLYRIVGMLGFKAGLLSTVVNQVVDEQIPTTHTTPDPI
                     ELNRLLRRMVDAGCSYCFMEVSSHAVVQKRIAGLTFAGGIFTNITHDHLDYHKTFDEY
                     IRAKKMFFDQLPSESFALVNADDRNGMVMVQNTKAKVSTYALHSIADFRCRVVESHFD
                     GMLLNMDGLEVWTRLIGEFNAYNLLAIYASLLLLGFDKTDVLLQLSMVNSVSGRFEYV
                     SSSGGITAVVDYAHTPDALVNVIETIKKIKSSGQRLITVVGAGGNRDKTKRPVMARVA
                     VQNSDLVILTSDNPRFEEPEDIINDMKAGVDEEFTKKLVTIVDRHEAIRTACLLANKE
                     DIILVAGKGHENYQEVKGVKHHFDDKEVLAEIFNELHI"
     gene            complement(10147..12270)
                     /locus_tag="PK839_08650"
     CDS             complement(10147..12270)
                     /locus_tag="PK839_08650"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HOG72843.1"
                     /translation="MSIKKDIVTRITVVYILFALLGVGIFVKIIYIQFVEGSKLREKA
                     KAITYRDIIVEPNRGDILASDGRVLATSVPYYDIRMDLAASGLSDQVFFSNIDSLAIC
                     LSRFFGDRSKYAYRSELVNARKYAHNKRFYPIAPRRVNYMELQTIKTFPLFRLGENKG
                     GFIAVQANRRILPHSKMAARTIGGVNQTGVAVGIEGAFDYALKGKAGLKVHERIPGNS
                     WVEVKSMNQIDPEDGCDVVTTIDVALQDVAEAALRKHLGNHDADHGCAVLMEVSTGDI
                     KAIANLKRNEDGTYSEVYNYAIGESTEPGSTIKIATLINLLEDGFVELNDTVNTGNGK
                     LRMYDRIITDSKEGGHGKISVKEVFEVSSNVGVAKLVTKYYRGKESHYVDRLYSMKLN
                     QPLGIGIPGEASPQIKYPTDRYWSGMSLPMMSIGYELKLTPLQILTFYNAIANDGRMV
                     KPRLVKSLERHGMVVQTFSPEVINPSICSRSTLRKVNEVLEGVVESGTAQNLKSTPYK
                     IAGKTGTAQLAKGRRGYKIGGSVSYQASFVGYFPSDKPRYSCIVVVNAPSQNIYYGNL
                     VAGPIFKEISDKVYATTPDWFREIDNGGSLKELPESKSTRLVMLKEILKQLDVPYQIA
                     NSKAEWVSTKRDSNEIALNTLSFSSKAVPSVVGMGLRDAIFLLENTGLHVSFNGRGTV
                     RSQSIAPGTAIKRGSNINLELSMSN"
     gene            complement(12290..12661)
                     /locus_tag="PK839_08655"
     CDS             complement(12290..12661)
                     /locus_tag="PK839_08655"
                     /inference="COORDINATES: protein motif:HMM:NF039650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsL-like putative cell division protein"
                     /protein_id="HOG72844.1"
                     /translation="MSVSGETEEFVDIQPEKPREKKGYRFKDFLSGRILTHENVSSQL
                     PYILFLVLLAVIYIGNSYRNNQLLRNEQKIKNEVKNLRAESITTAAKYMSVSRQSEVV
                     KLVEIKGLGLEESTIPPKKID"
     gene            complement(12658..13557)
                     /gene="rsmH"
                     /locus_tag="PK839_08660"
     CDS             complement(12658..13557)
                     /gene="rsmH"
                     /locus_tag="PK839_08660"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009120313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HOG72845.1"
                     /translation="MTYHVPVLLAESIEGLNIRPDGTYVDLTYGGGGHSRHILERLGK
                     KGKLYAFDQDEDALTNASDDKRFTLIRGNFKYFRNFLRYYNVAKVDGILADLGVSSFH
                     LDTPERGFSFRFEGPLDMRMNKRASLTAKKVVNSYSIEELSLIFNQLGELPDGRRYAQ
                     AIYDGRKVKQLETIEELTQILKPLVPAKLQNKVLAKIFQALRIEVNHELDALKLMLVG
                     SAKCLGREGRLVVISYHSLEDRLVKNFLKFGNFEGEDRPDLMGNRVVPFEQVNRKVIV
                     PSFDEISANNRARSAKLRIGRRL"
     gene            complement(13554..14021)
                     /locus_tag="PK839_08665"
     CDS             complement(13554..14021)
                     /locus_tag="PK839_08665"
                     /inference="COORDINATES: protein motif:HMM:NF014439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HOG72846.1"
                     /translation="MSSFIGDYECKVDEKGRILFPASLRKQVKSALPDQFVVKKDIYE
                     DCLTLYTMEEWQRQNEIIRTNTNPYNREHNAFLREFFRGTAEITLDSSGRLLLPKRLL
                     DMIGVVRDVVLAGMDGKVEVWAKEKYEGIRLESVQFASLAEKILGSTTIRDEK"
BASE COUNT         3702 a         3588 c         3176 g         3780 t
ORIGIN      
        1 cggaacttgt ccgcctactg gcggattctc ccttctacag tctcgggatt cagcatttcg
       61 gttcctggtg cacggttcac atttcgctat cggtttcctc aaaaagtcct gaaatcatct
      121 gcttaattgc gttgaacttg ctgccctttt tttcatgcac ctctgagaac tccttgtcaa
      181 cagatggttc ggtggcattt tgttgtttgg catcatcctg cacaggagtg gcagtctgtc
      241 tttcgagtcc gattagcgcc aaacccaatg cggtggatag ctggggatgg ttgtattttt
      301 caagggagtc ggacgaaagt ttctcactag ggaaccccaa ccggacatcg taacccgatt
      361 taaacttaat gaggtgcgag aggtgtttca gcagcgctcc gcctccggta atcaccacgc
      421 cggcgctaag cttctctgca tagcccgaaa ggttaatttc gtagaggaca aaatccagaa
      481 tttcctccat gcgggcctga atgatgcctg ccaggttttt aaccgaaatt tccttgggct
      541 cccgcccgct aataccagga atggtaatga ttttgttatc aggtgcaatg tcggagaggg
      601 ctataccgta gatttccttt agctggtcgg cctgtttcag aagaatggag caaccctcct
      661 taatgtcctg ggtgataacg tttccaccaa aaggaataac agcagtatgc cttatgttcc
      721 cctcgtagta tattgccagg tcggtagtgc caccgccaat atcaataagg gcaacgccag
      781 cctcacgctc gtcgtcggtt aaaacggcct cggcagaggc aagcggctca agaataagat
      841 cggccacact caagcccacc cggtttacac acttctcaat gtgcttggcc gatgaagttt
      901 taccgattac aatgtggaag ttggcatcta accttcggcc catcatccca acaggccggt
      961 tattgtcaac gggctccttg tcaataaaaa actccagggg taaaacatgc agtatggttt
     1021 caccggcatc aacaggagtg agattcatat cctttacaag cctgtcaata tcgtcctggg
     1081 agatttccct gtcgtcggta tcaaagttca ggtagttgct gttcttgata cttcggatgt
     1141 gctgaccggc aatcccaaca taggctgaat ggaattcgag tccaacttgc tttttaacgt
     1201 cgtcaactgt tttctggatt gccttaacgg tttcctcgat attttgaatc atccctcgtt
     1261 taatgccagt tgattcggcg gtgctaaggc ccaccacctg cagcttcccg ttggaattca
     1321 tcttacctac catggttacc acctttgtgg ttcccaggtc aattgccact acatagtcgt
     1381 ttgaatgtgc catatcacta acgttttgtg caaactattt gattagagta cataaggttt
     1441 attctgctat acttgttcca accctcggtt ggcagtgcgt tctcgtagaa aagcttcaac
     1501 tttttcaact tacggtcgta gccctccagg ctccccatag caataacatg aggaccaaca
     1561 acgggaatca gctcaacgtt tcgggcattg ataacgtaga tctggttgat ttgagccctc
     1621 caaaaggcat cgtggttgat atacctggca aagtcaaaga tttggcatac caacggtttt
     1681 gaaccattgg taaggctatc gttccacctg gtaatgtcaa tgtttggttt aaccctgaaa
     1741 ggttcatata tatttccgct cgcaataaga acataggctg catagctact cgaaacgggc
     1801 attaccaagc cctcgtcatc aatatagtag ctttgatttc gtgcgttgaa tatgcgaaca
     1861 atgggtctgc gctgtttcac cgtaattctg aggatgccat ccatggattt atatacctgc
     1921 acatcacgaa cggcaaccag ctcggaaatg tttttttcaa gcttatgggt gttaatcgaa
     1981 ttcaacggtt taccgatggg ggaaatagtg gacgaaagga tcatttttct tattgtaccg
     2041 ggtttaacaa actggtactg ggcgctatca acaacttcaa ccctgatgcc cctgcaaaga
     2101 accttttttg cacgctcggc agtaaataaa aggctataaa cgaggtatga gcctaacaga
     2161 agccatccca agataataag tatgttgcgg gttcgacggt tcatatctat ttagcctcaa
     2221 atatttttac aatttcgggc gccaaccggt caatatcgcc agcacccatt gtcaaaagca
     2281 catccacatc atttttccta agccactcga gcaggtcggc tttgtccaga agcagcctgt
     2341 ttggattgtt cattttatca gcaatggtct gcgaggttat tccttcaata ggttgctccc
     2401 tggcggggta tatatcgagc aaaatagcca tgtcgagctt atcgagcgac cgggcaaaat
     2461 cgaaagcaaa atcccgggta cggctgtaga ggtggggctg aaaaatcccg gttattttac
     2521 ggtttgggta aacctgcctg gccgattcta tcatggcctc aatttcgcgg gggtgatggg
     2581 cgtaatcgtc aacatatacg gttctactgc ttttaaaccg aatatcgaac cgtcgtgata
     2641 cgccagaaaa tgagagcaga ccgcttcgta gctcatcatg gctaatacca caggtaattg
     2701 ctgctgccgc agcggcaata gaattttcga ggttgaacaa tcccggaact ccaagcttga
     2761 ggtcgtctat cgttccatta ggcgtttcga gcgaaaagcc gtaacaaccc tcgttaaggc
     2821 taaggttgac cgggtagaaa tcggcatctg acgacagccc ataggttaac cgggtaacgc
     2881 ccctttcagg gataggcgca attcccagac cggatttagc tatcaacgtt ccgttaggtt
     2941 taacctgacc ggcaaactgg ttgaacgcct ccactaccct ttcataatca ccgtaaatgt
     3001 cgagatgatc ggggtcaacc gatgtaataa cggccagctg ggggtgcagg ttcaaaaaac
     3061 tgcgatcgta ctcgtcggcc tcggccacaa gcagctttct acccttatcg gcggtaacca
     3121 ggttgctgcc gaaatttttt gagattccac ccaaaaaagc atcgcaacca accgatgagc
     3181 ttcccagcag gtaggcaagc agggttgagg tggtagtttt gccgtgggtt ccggcaacgg
     3241 ccaatgtttg ataccctaat gacagcattc caagcacctg ggcgcgtttt aaaagctgaa
     3301 atccacccct gcgaaagtag gctagctccg ctaaggtttc gggaacagca ggagtgtaaa
     3361 caaccaacgt ggttgcagga tttctaaatt cagagggtat cagctctgca tcgtcggtaa
     3421 aatgaatgcc aatgcccatc tcggccagtt gcgatgtgat ggcggaaggg gtacggtcgt
     3481 acccggccac aacccgaccc ttaagggcaa aatatcgtgc cagcgcactc attccgatac
     3541 cgcctatacc caaaaaataa acccccaaat actgctccat gctcatatct agcgccttac
     3601 accttaagtt ttgccctaat caatttattt aacacgctga aacactgtat ttaaacatct
     3661 ctttcaccta caaagattta ctgttagcat tagccagcga tagcacctca ccagcaatca
     3721 gtgttgccga gtttggcaac gccattgtta aaatattctg tgccagttca cgcaatttct
     3781 cctcgttgga gataagcgaa aaggccgttt cgacaagtgt aggttctgca tcgctatcgg
     3841 caaccattac ggcagcattc cgtttaacca gagcttcagc gttgtgggtt tgatggtctt
     3901 cggccacatt aggcgatgga accagaattg ccggtttgcc cacaaggcag agttccgata
     3961 tggttccggc tcctgccctt gaaataacca gatcagctgc tgcaaaggcc atatccatcc
     4021 ggtttataaa atcgaaaagc ctgatattcc taccggagta acctgccaac tcctggttga
     4081 tggcttcgta gtaaaacttc ccggtttgcc atattaccgc cacctcgggg tgacgggcaa
     4141 ttgcatcaag cttatgccgc aggctctgat taatggttcc ggcaccgagg cttcctccca
     4201 acacaaggag aactttctgc tcgggagaga ggttgaaaaa tctaaacgcc tcttcccttg
     4261 ttaccttaag agtttcaatg tcctgccgca ccggattgcc tgttttaata atcttttcct
     4321 tcgggaagta gcgttccatg ccgtcgtatg ccacgcaaat tttgtgagca ccagctgcca
     4381 gcatcttgtt ggtaatgccg gcgaaggaat tctgctcctg tatcagcgtt ggtatccctc
     4441 gtttttgagc tgccctgaga atgggcccgc tggcatagcc gcctacacca accaccacat
     4501 cgggtctaaa gtcggctata attttacgag acaagcgaag acttctaatc agcttaaaaa
     4561 ccagcataat attctggggt gaaaggcttc gcctaatacc gattatgggc aggccaacaa
     4621 tacggtatcc tgcctgcgga actctttcca tctccatacg gcccaacgct cctacaaaga
     4681 gaatttcggc ctcgggcgac agctctttca cggcgttggc aatggcaata gccgggaaaa
     4741 tatggccgcc ggttcccccg ccgctaatta ttactttcag ctggttcttc tccatcaaaa
     4801 agttcctttt tactttgtaa cctacttaca ctcaaaataa taccaaacgc agcgcttgaa
     4861 aaaaacagcg aggttcctcc catgctaatc attgggagcg gttgcccggt aacaggaata
     4921 aggtttaccg ccactgccat attcaccatg gcctgcatgg tgaggagaag cgccaaccca
     4981 aaagccagga aagccggaaa agtacgcttg gcctttttga ctatcaccgc cgagcggtat
     5041 agaagaatta agtatagact gatgatgaac agcccagcaa aaagtccata ctcctcaatg
     5101 atgatggcgt atataaaatc ggagtacgga tggggtagga aatttctctg ggttgacctg
     5161 cccggccctt taccggtgat accgccgttg gcaatggcaa ttttcgattg ctcaacctgg
     5221 tagttaccgt cagggttatc gccactaaca aaattttcga tacggttttt ccatgtatgc
     5281 accctaccca catctccaaa atgaagggct accacaacaa aaatggcaaa aagggcaaca
     5341 ccaattcctg taaacccgag aagatatttc agatttaccc tcccaacata catcataatc
     5401 cagcatgtaa gccccaaaag tgcagcggtg gaaaagttgg caggaagaat aagcccacaa
     5461 atcaggatta cgggaagtgc aagcggtaaa aatccctgct taaggtcctt tatatccttt
     5521 tgcttctgag aaagtatttt ggccatgtac atcaccaggg caaacttagc cacatccgac
     5581 gactggaatg ttatgcccag cccaggtatt tcgagccagc gggaggcttc attcaacctg
     5641 gttccaaata ccagcgtaaa gagcaacagg ggaatggaaa taagcataag aagcgacgac
     5701 agcgggtagt aaaccctata gctaatcaga tgggtaacaa atacaattga tagggccagc
     5761 accaaaaaaa caagctgctt taccaaaaag taggtggtgt tgccgccatg gtaacgaaag
     5821 gccagcgagc ccgttgagct gtaaactacc agcagggaga atgccgataa cagaattacc
     5881 accagccata ttgccctatc gcccttaaag tatttatcta caaaagtccc cattggtata
     5941 atgcttaaag cattctaact gcatccttga atttacgccc tctatcctca tagttttcaa
     6001 acaaatcgaa gctggcacag gcaggtgaaa gcagaaccgt gtcgcccgga ttggccagct
     6061 cgtaggcact tctaacggca tcttcagccg acagcacatc aaccataacc gggacaacat
     6121 tcccaaaagc cttatgcagc ttgacgttat caacaccaag gcaaacgatt gccttaacct
     6181 tcaactttac caagtccatt agctctgagt aatcgtttcc cttgtcaacc ccgccggcaa
     6241 tccataccac gggcgtatcc atactctcca gagcatacca agccgagtta acgtttgtgg
     6301 cctttgaatc gttgatgtac ctgactcctt taatagttaa aaccggctca aggcgatgct
     6361 ctaccccctg aaaatcggat agcgactgcc ggattacatc cttacggatg ttcagcacat
     6421 gcccggcaat gcctgcggcc atagagttgt agatgttatg cttaccctta agcgaaagat
     6481 cgttaattga catgctgaag tcgctattgt tatagttgat tagtaggtta tcatcgtcga
     6541 gccatgcagc ttggttttcc ttaacacgct gggaaaaggg cagctgctgg gctctgagga
     6601 taatgcgttt gagctgctca acggtaacgg catcgtcgga acaaaaaatg aagcaatcat
     6661 cctctgatag gtttcgggtg attctgaact tcgattccac atagttctgc atcttgtagt
     6721 cgtatcggtc gagatgatca ggggtgatgt tcagcagaat ggcaatgtcg ggtttgaact
     6781 cgtaaacgcc atcgagctgg aagctgctga gctcaataac gtagtagtcg aaattgtctt
     6841 cggccacctg gtaggcaaag ctgttgccta tgtttccagc caggcccaca tttagccccg
     6901 ccttctgcat catgtggtat atcaacgagg tagtggtggt tttgccattg ctgccggtta
     6961 tgcagatggt tcgggctgtg ttgtatcgtc cggcaaactc aatctccgaa ataacaggga
     7021 taccctgctc cctaattctt gcaacaattg aagccttatc gggaatgccg ggacttttaa
     7081 tcacctcgtc ggcgttaaga atgagcgcct ctgtgtggcc gttttcctca aaaggtatgc
     7141 tgtacctttg cagccactca cggtagcggg gctttatggt tgatgaatcc gatagaaaaa
     7201 catcaaagcc cttcttgaat gccagcacag ccgctccggc accgctctct ccacctccca
     7261 gaatagctat tcgcttcatt ttaaagaaat atttgtattc attcgtttcc ggctagctgc
     7321 agccatctgc cacggtagcc aattgtaggg tatcctccct acgttaacct ttttataccg
     7381 tatgatacca tttagcccca tatttgcacc agctaaacac atatagctac ctgattttta
     7441 aagttaccac ggtgataaca gctagcaaca tgcttataat ccaaaacctg gtaacaattt
     7501 taggttcggc ataacccttt ttctggtagt ggtggtggag aggcgacatg agaaataccc
     7561 ttcgtccctc cccgtaaatt ttccgggtaa ccttaaagta gctgacctgg atgagaactg
     7621 aaagcgcttc aaccagaaag attccacaga gtatgggcag caggagttcc ttacgaacaa
     7681 caatggcata aacggctata atgccaccta tggtaaggct cccggtatcg cccataaaca
     7741 cctgggcggg gtagctgttg taccagagaa atccaataag cgcccctatg agcgcgctca
     7801 tgaaaacaac caactcacta atgtgcggta tatacatgat gttgagatac tcagagtaaa
     7861 taacgttacc cgagaggtag gcaaaaaccc caagtgtggt ggcattcatt gccgaaacgc
     7921 cagtagccag cccgtcaagc ccatcggtga ggtttgtgcc atttgaaact gcagtaataa
     7981 tgaaaataac aactacaatg aagatagtcc aggtgatggc atcgcgccac ttccccttga
     8041 aagggtttag gtacgcgtag tcaaactcgt tgttcttaaa gaagggtatg gtggtttggg
     8101 ttgttttttt gcccttacta acgctaacgg gttccgaacc ggagtactcc gaggtaacca
     8161 ccacttcgct ttgaatattt ggtcttacat ggtcgcgaat cataatgtcg gggctgagcc
     8221 acatagtgag accaacaaat agccctagta gaacttgtcc taaaattttg aacttacccg
     8281 aaaggccatc cttgtttttc aggaatactt tgatatagtc gtcggtaaag ccaactgcac
     8341 ccatccaaac ggtggttacc agcattatga tgatgtaaat gttaaggaga tcggcaaaaa
     8401 gcagaaccga gatcagggtg gcagcaatga cgatgattcc ccccatggtg ggtgttccct
     8461 ttttctgcag ctgcccctca agcccaaggt tgcgtatctc ctcaccaatc tgctttcgtt
     8521 gcaggtacct gatgattcgc ttgccaatga aaagggcaaa aagaagggac aggataaaag
     8581 ccagggccga gcggaacgaa atgtagcgga aaagaccggc tccggggaaa tcgaattgct
     8641 tttgaatgta ctgtaccagg tagtaaatca tatatgcagc tcgttaaata tttcagctaa
     8701 aacttcctta tcgtcaaagt ggtgctttac ccctttaacc tcctgatagt tttcatggcc
     8761 tttgcctgca accagaatga tgtcttcctt gttggccagc aggcatgccg tcctgatggc
     8821 ttcatgtcta tctacaatgg taactagctt cttggtaaac tcctcatcaa caccggcctt
     8881 catatcatta ataatatcct cgggctcctc aaaacgggga ttatcggaag taagaatgac
     8941 caagtcgctg ttctgaacag ccactcttgc catgacaggt ctcttagtct tatcgcggtt
     9001 tccaccagcc ccgaccaccg ttataagccg ttgtccgcta ctcttaatct tttttatagt
     9061 ttcaattacg ttaaccagag catcgggggt gtgggcatag tccaccactg cggtgatacc
     9121 gccacttgag ctaacatact caaagcggcc gctaaccgaa ttaaccatgc ttagctggag
     9181 caatacatcg gttttgtcaa acccaagtag caaaagcgag gcataaatag ccagcaggtt
     9241 ataggcgtta aactcaccga taagccgggt ccaaacctcc agcccatcca tattcagcag
     9301 cattccatcg aagtggcttt caaccaccct gcagcggaaa tcggcaatgg agtgaagggc
     9361 gtaagtagag actttggctt tggtgttctg aaccatgacc attccattcc gatcgtcggc
     9421 attaaccagg gcaaatgatt cggaaggcag ctggtcgaag aacatttttt tagccctgat
     9481 gtactcgtca aatgttttat ggtagtcgag atgatcgtgg gtaatgttgg tgaatattcc
     9541 gccggcaaaa gtaaggccgg caatcctttt ctgcacaacg gcatgtgagc tcacctccat
     9601 gaagcagtag ctacaccccg catcaaccat acggcgtagt aacctgttca attcaattgg
     9661 gtcgggagtt gtatgcgttg tgggtatttg ctcgtctacc acttggttca ccactgttga
     9721 gagcaagcct gccttaaatc ccagcatccc aacaattcgg taaagtagag ttgccgtggt
     9781 ggttttacca ttggttccgg taacacctac cagcttaagg ttcgacgacg ggttaccgta
     9841 gaatgcagaa gccattagcc ccagggcttc cgaggaatcc gaaaccctaa cgtatgttac
     9901 atttggggca aagctaacgg gcattgcttc gcacacaata gctgaagccc cgttttcgac
     9961 tgcttgaggg atgaaccggt gcccatcagc ctgagttccc cttatggcaa aaaagcaaga
    10021 gttggccccg gcaaccctcg agttgaaggt tagctgttcc acctcgatat cggggtttcc
    10081 aactacttcg ttgacaagcg cttttggtaa tattttagtt aagcctactg ccatgatata
    10141 taaattctaa ttgctcatac taagttcaag gttgatgttg cttccccgct taatggctgt
    10201 tcccggggca atggattgcg aacgaacagt acccctcccg ttaaagctaa catgcaaccc
    10261 ggtgttttcg agaagaaaaa ttgcatccct aagccccatc ccaacaaccg acggaacagc
    10321 ttttgatgaa aaactaagcg tgttaagcgc aatctcgttg ctatcgcgtt tggttgaaac
    10381 ccattcagct ttactgttag caatttgata aggcacatcg agctgcttca atatttcctt
    10441 tagcattacc aatcgggttg atttagattc ggggagctcc tttaggcttc caccgttatc
    10501 aatctccctg aaccaatcgg gcgtggtagc atacaccttg tcggaaattt ccttaaatat
    10561 tggcccggca accaggtttc catagtagat attctgcgaa ggtgcgttaa ccaccactat
    10621 gcaggaatat ctaggcttgt ccgaaggaaa ataacccaca aacgatgcct gatagctaac
    10681 gctaccgcca attttgtagc cccttcgccc cttggcaagc tgtgctgttc ccgttttgcc
    10741 tgcaatttta tagggagtac ttttaagatt ctgggcagtg ccgctctcaa ctaccccttc
    10801 cagcacctcg ttcacctttc ggagggttga cctgctgcaa atggaggggt tgataacttc
    10861 gggcgagaag gtttgtacaa ccattccgtg tcgctctagc gacttaacca gccttggctt
    10921 aaccattctc ccgtcgttgg caatagcatt gtaaaaggta agaatttgaa gcggtgtaag
    10981 cttgagctcg taccctatgc tcatcattgg taacgacatc cccgaccagt acctgtcggt
    11041 ggggtacttg atctgtgggc tagcctcgcc cggaatgcca attcctaagg gttggttgag
    11101 cttcatggag tagagcctgt caacataatg gctttccttt cccctatagt acttggtaac
    11161 cagcttggcc actccaacgt ttgatgaaac ttcaaacacc tccttaaccg aaattttacc
    11221 atgccccccc tccttgctgt cggtaattat gcggtcgtac atccgcaact tgccgttgcc
    11281 ggtgttaacc gtatcgttca attcaacaaa gccatcttcc agcaggttaa tgagggtagc
    11341 tattttaatg gtggagccgg gctcagtact ttcgccaatg gcgtagttgt aaacctcaga
    11401 gtaggtacca tcctcgttgc gcttaagatt tgcaattgcc ttaatatcgc cggttgatac
    11461 ctccatgaga acagcgcaac catggtcggc atcgtgattt ccaaggtgtt tacgaagtgc
    11521 cgcttcagca acatcctgta gggcaacatc aatggtggta accacatcac aaccatcctc
    11581 cgggtctatc tggttcatac ttttcacctc aacccaacta ttcccaggaa tacgttcatg
    11641 caccttaagc cctgccttac ccttaagtgc atagtcgaat gctccctcaa tgcctacggc
    11701 aacaccagtc tggttgacac ctcctatggt gcgtgcagcc attttggagt gtggcagaat
    11761 tcgccggtta gcctgaactg cgataaaccc acctttattt tcacccagtc gaaacaacgg
    11821 aaatgttttt atagtttgca gctccatgta gttcaccctg cgcggggcaa ttgggtagaa
    11881 tcgcttgtta tgggcatact tgcgagcgtt aaccagctcc gaacgatatg cgtattttga
    11941 acgatcacca aaaaatcggg ataggcaaat agccagagaa tcaatatttg agaaaaatac
    12001 ctgatcggac aacccactgg ctgccaaatc catccgaata tcgtagtagg gaacagaagt
    12061 ggccagcact ctcccgtccg atgccagaat atcaccccgg ttaggttcca caataatatc
    12121 cctgtaggtt atggctttgg ccttttccct aagcttcgac ccctcaacaa attgtatgta
    12181 gatgatttta acaaaaatac caacgcccag cagtgcgaat agaatgtaaa ctactgtaat
    12241 tcgggttact atgtccttct taatcgacat acctgcaggc tacttttggc taatcaattt
    12301 ttttaggggg gatggtggac tcctcaagcc ccagcccctt aatttcaaca agttttacca
    12361 cctccgactg acggcttact gacatgtact tggccgcagt tgtaatggat tctgccctga
    12421 ggttttttac ttcattttta attttttgct cgtttcgcaa cagctggtta ttccggtagc
    12481 tattgcctat gtagattacg gcaaggagaa ccaaaaaaag tatgtagggc agctgtgacg
    12541 aaacattctc atgggtgaga atccgcccac ttaaaaagtc cttaaaccga taacccttct
    12601 tttccctggg tttttcaggt tgaatgtcaa caaattcttc agtttcgcca cttacactca
    12661 tagccttctt cctattctta acttggcgct acgcgctcta ttgttagcag aaatctcatc
    12721 gaacgaggga acaattacct tccggttcac ctgctcaaag gggacaaccc tgttacccat
    12781 taaatcgggt ctatcctctc cctcaaaatt gccaaacttg agaaagtttt ttaccaggcg
    12841 gtcctccagc gagtggtacg aaataaccac caaccgccct tctctcccga gacatttagc
    12901 agagccaacc aacattaatt taagcgcatc cagctcatgg tttacctcaa tccgcaaagc
    12961 ctgaaagatt ttggccaaaa ccttattttg aagtttagct ggaacaagcg gtttcaaaat
    13021 ttgtgtaagc tcttcgatgg tttccagctg ctttacctta cgtccatcgt atatggcctg
    13081 ggcataacgc ctaccatcag gtaactcacc aagctggtta aaaatcaggc ttaactcctc
    13141 gatgctatag ctgttcacca ctttttttgc cgtaagtgag gcccgcttgt tcatgcgcat
    13201 atccaatggc ccctcgaatc ggaacgaaaa tccgcgttcg ggagtatcca ggtgaaacga
    13261 tgaaactccc aaatcggcca gaataccgtc aactttagcg acattatagt accgtagaaa
    13321 gttcctaaag tacttgaagt ttcccctgat tagggtaaac cgtttatcat ctgaagcgtt
    13381 tgtaagggca tcctcatcct gatcgaaagc gtaaagcttt ccttttttcc caaggcgctc
    13441 aagtatgtgg cgggaatggc cacccccgcc ataagtgagg tcaacgtacg tgccatcggg
    13501 tctgatgtta agaccctcta tgctttcggc aagcagaacc ggtacatggt aggtcatttc
    13561 tcatccctta tggttgttga acctaaaatt ttttcggcca gactggcaaa ctgaacagac
    13621 tcgagccgta tgccttcgta cttttccttt gcccaaacct ccacctttcc gtccataccc
    13681 gccagcacga cgtcgcgtac cactccaatc atatcgagca gtcgctttgg tagcaatagc
    13741 ctaccacttg aatcgagcgt gatttcagct gttcccctga aaaactcccg aagaaaagcg
    13801 ttgtgctccc ggttgtaagg gttggtgttc gtccttatta tttcgttttg ccgctgccac
    13861 tcctccatgg tgtagagcgt aaggcaatct tcgtaaatat ccttcttcac aacaaactgg
    13921 tcgggcagcg cagatttgac ctgcttgcgt agcgatgcag gaaagagtat tctacccttt
    13981 tcgtcaacct tacactcgta gtctccgatg aatgaggaca tttccagctg gcatttaaat
    14041 ggtaaaaata tacaatactt taccactttt atccactttt acccactttt ttgcattttg
    14101 ttgataactt ttttttgaaa gcgacgtacc taggccaatc ggtgagatgt tagccgtcaa
    14161 cctttaagga taaacccatt agctgatgga cagtttacgg aatccgtcag gagccggaca
    14221 agttccgcca gtaggcggac aagttc
//
LOCUS       DAOSHM010000060        13877 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_3662_length_13877_cov_21.200333, whole genome shotgun
            sequence.
ACCESSION   DAOSHM010000060 DAOSHM010000000
VERSION     DAOSHM010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 13877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13877
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3662_length_13877_cov_21.200333"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1459
                     /locus_tag="PK839_08670"
     CDS             <1..1459
                     /locus_tag="PK839_08670"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOG72847.1"
                     /translation="MPSKSFIKSSFIYTVVGSLPLATSILLLPFYGNSSLLSTADFGL
                     LAIYIILSELAKILFTFSADSFLGFNYIHYNNSKSNLQRFIGTTSVFMLVYGVLMLLL
                     FCVLGGSAIKLLYPGKNISFYPFGFLSVLTGLLNGIFKAYSTLMIYREKPAPFLWSNI
                     IHFTLVLAVSITGLYLYPQTLLGPIWGRFVGAIATFLWSLTYFVSASRFRVDFNILKK
                     LLSYSAPLYLFGILYWIVSNIDRYFILGLLSEKDVAIFDFAVKLTLAIEFLQNGLSSA
                     IYPKIFQIWKKNDDVPHGNVEINRFFHVFALITVVGIPVLYLSVPLLVPLLVNNSELY
                     ASFNLLPLLFAGMVSRIWYYYLVAPIYYFKQTKVMPLVFGFAAIFQIASTYILIQLNG
                     IDGAVWANLLTKLFQVFLLYLFVRRFYTLRVNVKKLIVFPAVYMMLLISAEIFLKPVN
                     IYLLNLIHIVIIGVLAYSFFRNELKWENIRTALKK"
     gene            complement(1469..3739)
                     /locus_tag="PK839_08675"
     CDS             complement(1469..3739)
                     /locus_tag="PK839_08675"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOG72848.1"
                     /translation="MERAYSKPFVVRITILGFIAGLAVILLAFFIAAVTGGYSFNFSG
                     IVEMHRIMPVLYFLDIFPLLIALASFYISRYISRIRMESESSSEETIHRQRKLYHFVE
                     KLQEGDESVEYIPDEGDALGKAIVNLRNNISQSRKEEEIRRKEDEQRRWSAEGMARFG
                     EILRLNNDNVEELSFQIIRNLVNYVGANQAAFYLLEDDDEADVHFRMTACYAYERRKY
                     ADRIVPWGDGLVGACALEKETIFLKKVPDSYLNITSGLGKANPRCLVLIPLKLNDQVH
                     GVLEVASFNIFEKHQLEFMEKIAESVATTISTVKINLRTAKLLRESREQAEELALKEE
                     QMRQNMEELQATQEEAARQSEKFISFSNSVNHTMIRADFDVKGTLTYANTKFMQKLEY
                     SSTAEVEGKPLDIFLDRRDRVWFEEVWKKLSKGGKHFEGNMKLATKTGKELWILATFT
                     CMRNTMGDVDKILFLALDASGQRAKNLELEGQVNAFSRSVVKADISPTGDMIAVNDMY
                     IETLGLPYEVAMKKNIFETVSPVDRQNVEDAWDDIIHGNPFDGKLRVNTGNNDEKWLQ
                     VSLVPVNDTNGEIAKVLLIAYDITSEVHLAIEVQQKEETIRKQEELIQQKEVEQNKKL
                     RETREEITNQYRETEKIKVRNEKLLEELSEAIVTTDHDGLILFMNKAAENLFTVKHDE
                     VANQNVRILFSDTVKDNFVTTYLDPARKKIVGQRQMVTLNPRKGKEVNATMVVYEVKT
                     GREITYTAFFQPTTNS"
     gene            4451..4837
                     /locus_tag="PK839_08680"
     CDS             4451..4837
                     /locus_tag="PK839_08680"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOG72849.1"
                     /translation="MSRILIVDDIHVNRLLLKEILTKISVDCLEAKDGKEALEILQNE
                     KVDVVLMDIEMPVMNGVETTRYIRQNFPFPLKSIPIIALTAHNPADFFEDFHNTGFNY
                     LLTKPYSIERIQKAIDETLKQEKQQN"
     gene            5087..6307
                     /locus_tag="PK839_08685"
     CDS             5087..6307
                     /locus_tag="PK839_08685"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOG72850.1"
                     /translation="MTSRDLAQLIAAHSRLILPNLGAFLHKDAADETFSASKVTFSPF
                     LKYNDGKLEDYLSKTTGISKEESFKQVHALVSSINQQLESSGKYPIPGLGQLVRDNQG
                     AISFVADTSVNTDTIEANFSGKISNSPPKDDGKTGEESMITGDNKASSYNSTPANRAS
                     SIKINVEADTSNPDSTAKSAIEDSINKTDSEPEKQTQDKAENIGKLENEPVKGYFKMG
                     KRSSGKKKSKLLLPALFVITSLVVIFIIALVIRHIALSPSYNYSDEDVTPENDYTPGI
                     SNMTTPKPKDKIDRAYDSLVGQIKKEESNPSSPEPSIEDKIEQEVIENYKAKDGEPTG
                     KYYLIVGSYKNADNARNMASKLKDKGFKSSVVSRPDGISCVALGAYSSNGEAEKAKLA
                     YEKQFPGIWILTVQ"
     gene            6378..7112
                     /locus_tag="PK839_08690"
     CDS             6378..7112
                     /locus_tag="PK839_08690"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HOG72851.1"
                     /translation="MTSKSKPLSNSKKLCTYPLIGEVSYIRKSGLKRVSLRVNAYGDV
                     RVSMPPFISFKYAEEFMLSNGEWIIEKKGRSFLGKNLIDNDSIHTRYHTITLFEDSQS
                     TGIRVIQSNNQARIHYPLTMAKNDPAVREVLKKVVEHTYMKEAKDDLPLRTANLAAAH
                     GFSFRRVSISRATTRWGSCSPENNIRLSLHLMRLPDHLVDYVIIHELCHTVEKNHGIN
                     FWKLMNEHCNGHARQLAREIKEYHAY"
     gene            complement(7150..7431)
                     /locus_tag="PK839_08695"
     CDS             complement(7150..7431)
                     /locus_tag="PK839_08695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72852.1"
                     /translation="MSKIRDFKKEVKHMVKLFLNECYTQLSFSPEFNQENILDIISDV
                     IVLQRDTVSKLSRKNFAKGSRKNIDYQKVASEFYTQIVELTERLNSLDY"
     gene            complement(7617..8081)
                     /locus_tag="PK839_08700"
     CDS             complement(7617..8081)
                     /locus_tag="PK839_08700"
                     /inference="COORDINATES: protein motif:HMM:NF024304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOG72853.1"
                     /translation="MKSRSNLTPAEVNDIINRSLVCHVGMVDTNGMPYVLPFNFGYQN
                     DRLYIHSGPEGKKIGIWKSNPNVCVEFSTDYQMHHQNDSVACSYSMRYKSVLIYGMVK
                     EIDDIDKKKEVLEIFMLKYTSLQNFKYSTPALANVKVFEILPEKIEARAYGY"
     gene            complement(8299..11157)
                     /locus_tag="PK839_08705"
     CDS             complement(8299..11157)
                     /locus_tag="PK839_08705"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005388 - P-type calcium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HOG72854.1"
                     /translation="MVEIKYKGLSEEQVVESRAKFGENVLTPPVRKPWWRLLLEKFND
                     PIIRILTIAAVIAIAVGAVNGHYLEGVGIIVAIFLATFMSFINEYRAGKEFDILNQVN
                     EENPVKVIRNGGVTSIPKKDVVVGDIVIVDRGDEIPSDGVLLESVSLSVNESSLTGEP
                     IAQKSYDSSLDQGNNTYPINRVYKGTAVVEGHGIYETTSVGDSTEIGKTARESTLQVD
                     IQTPLTKQLNRLSDFIGSIAFLLAILIFVLLVVADVAKGTSGLSSNQQFILVSLVVAA
                     VVALMKFWLPSLNFLVKAFGAKRGIPDIFAKAKINSYLLSFLLGLAVVVVAIPIAFAK
                     GIDLADSANWFSVDFISKLLNYFMVSMTLIVVAVPEGLAMSVTLSLAYSMRKMTADNN
                     LVRKLQATETMGAVTVVCTDKTGTLTQNRMAVLESSFSAVDDISENSLNAELIKYSMA
                     VNSTAHLDFTEKSPAVIGNPTDAALLLWLNNQGINYQSIRNDFTIEQQLPFSSEKKYM
                     LTVGKAGSLNKRILLVKGAPEVVISMCEVDDILQAKLTGLLESYQQKSLRTIAFAYRF
                     LDHEVVNENEKPSNLNFIGVVGIADPVRLDAAEAVAQCHRAGVDVKMLTGDNIVTATS
                     IGKQVNILSGNAEANVISGPDFEALSEDEARRMANQLKIMYRARPSDKMRLVRLLQEN
                     GQVVAVTGDGTNDAPSLNRADVGLAMGSGTQVAKEASDIILLDDSFSSIVNAIRWGRT
                     LYGNIQKFLFFQLTINLLAVVIVLTGPFTGITLPLTVTQMLWVNLIMDTFAALALATE
                     QPHAGVLNKPPRSPKEFIVTPSIFKGILLASASFLIILLGMLIFFGRDGNVSSYELTL
                     FFNLFVMLQFWNLFNSRSLGDYSSALKGLFHNKGFITIAFVILLGQVVIIQWGGEIFR
                     TVPLSLSDWVKIFVASSLVLWIPEAYRFFKRISLKKVR"
     gene            11418..12134
                     /locus_tag="PK839_08710"
     CDS             11418..12134
                     /locus_tag="PK839_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOG72855.1"
                     /translation="MGRAFEYRKARKLKRWGNMAIVFTRLGKEITMVAKSGGADPDTN
                     PKLRLLIQNAKSENMPKDNIERAIRRATDKDTKEYKEVVYEGYGPYGVAILVETATDN
                     PTRTVANIRSYFNKHGGSLGTSGMLDFMFEHKCSFRIDGKDSISLDELELELIDYGVE
                     EVFVEDDSILIFADFAQFGPVQRYLEGKGLHILNFQFERIPTDTKKLTPEQQEEVEKL
                     VDKIEEDEDVTHVFHNMEIA"
     gene            complement(12184..13287)
                     /locus_tag="PK839_08715"
     CDS             complement(12184..13287)
                     /locus_tag="PK839_08715"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOG72856.1"
                     /translation="MDEKLIVIAIESYAKAVVLQSYLEANGIECFLRNENILQGAVSE
                     GVKVQIRESDAEKALRLLSEQKFSEEAKKREKPLRKILVPVDFSEPSQNAARFAVMLA
                     EKYNAEIKLLHVFNSPVVDMIPFADVSGIQIDFDINFNLLYKAAKEKLIKFHADLVEF
                     ARQMGFNSVSIGYTLQEGYAAYGIVEVSKRYKPGIIVMGTKGEGFKSTELVGSVASEV
                     SEETKVPLLAIPEKAVLKGVDEVKNVLYATNLDESDFGSIRKLIRILSPFNFNLHCVN
                     MTNRPDNVEIKALMSSLKNYLYQVNPELSVEYSIVECKDMVKSFIDYIYDKKINLVAL
                     TSVKRSLIYKLFNPSLSKKMLYQSNVPVLLFQA"
     gene            complement(13390..>13877)
                     /locus_tag="PK839_08720"
     CDS             complement(13390..>13877)
                     /locus_tag="PK839_08720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72857.1"
                     /translation="PTQSFDLTAEFGGYKLSVSIDYSDSSSEIYSLSKLQISFKQGNT
                     TLYALDLNLNVSMISETNTSVKLNATLIMNSLKFTATADFNTDDLDYETNPNVDLNEY
                     ISVKLYTTSDREIGHFSFVRNFETGELTVKFIYADGTEVNAEELMPQISAFIQSLLEE
                     E"
BASE COUNT         4187 a         3033 c         2693 g         3964 t
ORIGIN      
        1 gatgccctct aaatcattca tcaaatcttc gttcatctac acagtggtag gctcgcttcc
       61 actggctaca agcattctgc tgcttccatt ctacggaaac tcctcactac tttcaacagc
      121 tgacttcggg ttgcttgcca tatacatcat tcttagcgag ctggcaaaga ttctttttac
      181 tttttccgcc gatagctttc tgggattcaa ctacatacac tacaacaata gcaagagcaa
      241 cctgcaacgt tttattggaa ccacatccgt attcatgctg gtttacggcg tattaatgct
      301 attgctattt tgtgttttgg gcggctcagc cattaagctg ctctacccgg gcaaaaacat
      361 aagtttttat ccctttggat ttctttcagt gttgacgggt cttctcaacg gcatttttaa
      421 ggcatactca accctaatga tatatcgtga gaaacctgca ccttttttat ggtccaacat
      481 tattcacttc acccttgtgc tggcggtttc cattaccggc ctgtaccttt atccccaaac
      541 cctcctggga cctatctggg gacgcttcgt aggggcaatt gcaacatttc tttggtcgct
      601 aacatacttt gtatcggcca gccgatttag ggttgatttt aatattctta aaaaactact
      661 ttcctacagc gcaccgctct acctttttgg aattctttac tggattgtgt caaacattga
      721 ccggtacttc atcctaggcc tactttcgga aaaagatgtg gctattttcg actttgcagt
      781 taagctaacg ctggcaatag agtttttaca aaatgggcta tcgtcggcaa tatacccgaa
      841 gatttttcag atatggaaaa agaatgacga cgttccgcat ggaaacgtgg agatcaaccg
      901 attcttccat gtttttgccc taattacggt tgttgggatt ccagtactct acctctcggt
      961 tcccctgctg gttccattgc tggttaacaa cagcgaactt tacgcctcat tcaacctcct
     1021 acccctcctt tttgccggca tggtgagccg tatctggtac tactaccttg tggcgccaat
     1081 ttactacttt aagcaaacca aggtaatgcc actggttttt gggtttgccg caatttttca
     1141 aatagccagc acctacattc ttatccagct taacggtatt gacggtgcag tatgggcaaa
     1201 cctgctcaca aaactttttc aggtattcct tttatatctt tttgtaagga gattctacac
     1261 cctgagggta aatgttaaaa agctaattgt tttcccggca gtttacatga tgctactcat
     1321 ttcggcagaa atatttttaa agccggtaaa tatttacctg ctcaacctca ttcacattgt
     1381 aataattggg gtgttagcct attccttttt ccgtaatgag cttaaatggg aaaacatccg
     1441 gacagctttg aaaaaatagc ccaactctct aggagttggt tgtaggttga aagaatgctg
     1501 tgtaggtaat ttccctaccg gtttttactt cgtaaacaac cattgttgcg ttaacctctt
     1561 taccctttcg agggtttagt gttaccatct ggcgttgccc aactattttt ttccttgcag
     1621 ggtctaaata tgtcgtaaca aagttgtcct ttacagtatc ggaaaacaga attcgaacat
     1681 tctggtttgc tacctcatcg tgtttaacag taaataaatt ctcagcagcc ttattcataa
     1741 acagaattag cccatcgtgg tcggtggtta caatagcttc ggaaagctct tccagaagtt
     1801 tttcgttacg aactttaatt ttctccgttt ccctgtattg attagtaatt tcttcacgcg
     1861 tttcgcgtaa tttcttattt tgctctactt ccttttgttg gattaactct tcctgttttc
     1921 taatggtttc ctctttctgc tgaacttcaa tggcaaggtg cacttcactg gtaatgtcat
     1981 aagctatcag taaaaccttg gcaatttcac catttgtatc gttaaccggg acgagggata
     2041 cctgtagcca cttctcatca ttatttcccg tatttaccct tagctttcca tcaaatggat
     2101 taccatgaat aatatcatcc catgcatcct caacattttg gcggtcaacc ggtgaaacgg
     2161 tttcaaatat gttttttttc atggccacct cgtaaggtag tcccagcgtt tcgatgtaca
     2221 tatcgtttac tgctatcata tctccggttg ggctgatatc ggctttaact acagatcggc
     2281 taaaggcgtt aacctgacct tccagctcaa ggtttttcgc tctttggcca gaagcatcaa
     2341 gagctaaaaa gaggatttta tcaacatcac ccattgtatt tctcatgcag gtaaatgtgg
     2401 ccagaatcca tagctcctta ccggttttgg tagctagctt catgttaccc tcaaagtgtt
     2461 taccaccttt gctaagcttt ttccacacct cttcaaacca taccctatcc cgtctatcta
     2521 aaaaaatatc caaaggtttt ccttcaactt cggcagttga ggagtattcc agcttctgca
     2581 tgaatttggt gttggcgtag gttagggtcc ccttaacgtc gaaatcggcc cggatcatgg
     2641 tgtggttaac cgagttggag aagctgatga acttttccga ttggcgggca gcttcctcct
     2701 gggtggcttg caactcctcc atgttctgac gcatttgctc ctcctttagg gcaagttctt
     2761 cggcctgttc gcgtgattcc ctcagcaact tggccgttcg taggtttatc tttaccgtgg
     2821 aaatggtagt tgccacgctt tcggcaatct tttccataaa ctccagctgg tgcttctcga
     2881 atatgttgaa cgaagcaacc tccagcacgc catgaacctg atcgtttagc ttaaggggta
     2941 tgagaaccag acaacgtggg tttgccttac ctagtccgga tgtaatgttc aggtagctgt
     3001 ccggtacctt cttcaaaaaa atagtttcct tttcgagggc acatgcacca accaaaccat
     3061 cgccccaggg aacaattctg tcagcatact ttcgtcgctc gtaggcgtag caggcagtca
     3121 tacgaaaatg aacatcagcc tcatcgtcat cctcaagaag gtagaatgct gcctggtttg
     3181 caccaacata gtttaccagg ttcctgatta tttgaaatga tagctcctca acgttatcat
     3241 tatttaacct gagtatttcg ccgaaccggg ccattccctc agcgctccat cggcgctgct
     3301 catcctcttt tcgccgtatc tcctcctcct tacggctttg gctaatgttg ttgcgaagat
     3361 ttactattgc cttgcccagc gcatcaccct catccgggat atactcaacg ctttcgtctc
     3421 cctcctgaag tttttcaaca aaatggtata gcttccgctg cctatgaatc gtttcttccg
     3481 agctactttc ggactccatc ctgatacgtg agatgtagcg tgaaatgtag aatgatgcca
     3541 gagctataag caatgggaaa atatccaaaa aatataaaac cggcataatg cgatgcattt
     3601 caacaatgcc gctgaagttg aaagaatacc ctccagtaac cgctgcaata aaaaatgcaa
     3661 gcagaataac agctaaaccg gcaataaatc ctaaaatggt tattctgact acaaagggtt
     3721 ttgaatatgc tcgctccatg gcagtattat ttttgatttt aaaagtaggt attttttcaa
     3781 ttgaatggaa aaattcgatt ccaaatttag cgtgttacca gccaaaatcg aggccctatc
     3841 cgatacctat tcttcccaaa gtcagcccag ctataccgga taagtgcaaa tgaacaaaat
     3901 tcatagcaaa caaactaaaa tcaacagaac gtattaccag aactacctga tattttgaag
     3961 tatcaacaaa attgaaaaaa ataagcgcaa tacccgtaag cacagaatca actttaatta
     4021 ttgggtgtgc ctaaaaagta gtatttcttt aaccaaccat attcaggagg gaagccattg
     4081 gtttttccac agcacatacc atcgcacctg aaatttgcac tcaaaatcct gaaatctgcc
     4141 tacgaaacag cagcatttta gcctaaaata atactacaac acatataggt actgccacaa
     4201 ttagacctaa agcatccatg accagcagtc catttaagct accaaaacgc caaatatgtg
     4261 gggcttaaaa aaattgtttt tctctcactc tccgaaaggt catggcttgc ttccgcacca
     4321 taagatgagc acgcttataa ttcttccggt gattttttaa aaaaagttaa gctaaatagg
     4381 gaaaaattac gtatcataca gcattaccaa ccactaaaac attaaattgt attaagcaaa
     4441 acatacaaac atgagcagaa ttttaattgt tgacgacatc cacgttaatc ggctactact
     4501 aaaggaaata ctcacaaaaa tcagcgttga ctgccttgag gctaaagatg gaaaagaagc
     4561 cctggaaatt cttcaaaatg aaaaagttga tgtggttctt atggatattg aaatgccggt
     4621 aatgaatggc gtggagacaa cccggtacat tcgtcagaac tttccgttcc ccttaaaaag
     4681 cattcccata attgccctaa cagcccataa cccggctgat ttctttgaag attttcataa
     4741 caccggtttt aactacctgt taacaaaacc ctactccatt gaaaggatac aaaaggctat
     4801 agatgaaact ctaaagcagg aaaaacagca aaactgaatt ttcaaattcc aatattaaag
     4861 caaggcattc cttaacgttt aattattcag taaggcaagc ccttttccca gcaaatatag
     4921 gtaaaattga acagcacccc ggtttgcgaa taatatggat aaaccaactt attatagcct
     4981 attcgcaggt aataaccttc atttttttta aagagctcct tttactcctt actgtaaaat
     5041 atttttaact ttacagcgct atttcaatca agggaaaaag gtaaaaatga ccagcaggga
     5101 tttagctcag cttattgccg ctcactccag gctaattctt ccaaacttag gagcattcct
     5161 ccacaaggat gcagctgatg aaacgtttag cgccagtaaa gttaccttta gcccatttct
     5221 aaagtataat gatggaaagc tggaggatta ccttagtaaa acaacaggca taagcaagga
     5281 ggagtcgttc aaacaggttc atgcgctggt aagtagcatt aaccagcaac ttgagtcgtc
     5341 tgggaaatac cccattcccg gtttaggaca acttgtccgc gataaccaag gagctatttc
     5401 ctttgttgca gatacatcag taaacaccga caccatagaa gctaattttt ctggtaaaat
     5461 ttccaattcc cccccgaaag atgatggcaa aaccggtgaa gaaagcatga taaccgggga
     5521 caacaaggct tccagctaca attcgacacc tgcaaataga gcaagttcaa taaaaattaa
     5581 tgttgaagca gatacgagta acccagattc aacagcaaag agtgctatag aagatagtat
     5641 caataaaacg gatagcgaac cggaaaagca aacccaggac aaagctgaga atattggaaa
     5701 attggaaaat gaacctgtaa aaggatattt taaaatgggt aaaagaagta gcggtaagaa
     5761 gaagagcaaa cttttactac cagccctgtt tgtgataaca tcgctggtcg taatctttat
     5821 tatcgccctt gttattaggc atatagccct aagcccaagc tacaactact ccgatgaaga
     5881 tgtaacccca gaaaacgatt ataccccggg tatttctaac atgacaaccc caaaaccaaa
     5941 ggataaaatt gacagggcat acgattccct agtgggccaa atcaaaaagg aagagagtaa
     6001 cccttcaagc cctgagccta gcattgagga caaaattgag caggaagtta ttgaaaacta
     6061 caaggctaaa gatggcgagc caacaggtaa atactacctg atagttggca gctataaaaa
     6121 tgccgataat gctcggaata tggctagtaa gcttaaagat aaaggattta aaagttcggt
     6181 tgtttcaagg cctgacggta taagctgtgt tgccctagga gcatattcat caaacggtga
     6241 agcggaaaaa gccaagctag cctatgaaaa acaatttcct ggaatatgga ttttgaccgt
     6301 acagtagtca attttacaca tcgccggcta atttttgatg ttgaaccctc gtcaatttag
     6361 agacaaaata cttcacaatg accagtaaat ccaagccatt gtcgaatagt aaaaaactat
     6421 gtacctaccc attaattggc gaagtttcat acattcgtaa atcaggatta aaaagagttt
     6481 ctctaagagt taatgcctac ggcgatgtac gggtatctat gcccccattc atctcgttta
     6541 agtatgccga ggagtttatg ctctcaaacg gagaatggat tattgaaaaa aaaggcaggt
     6601 cctttttggg gaaaaatctt atagacaacg attcaatcca tacccgatac cacaccatca
     6661 cactatttga ggatagccag tcaaccggaa ttagggtaat tcaaagtaac aaccaagcca
     6721 gaattcacta tcctttaacc atggcaaaaa atgacccggc agttagagaa gtcctaaaaa
     6781 aagttgttga gcatacctat atgaaagagg caaaagatga cctgcccctc agaacagcca
     6841 atttagctgc cgcacacggt ttctcttttc gtagggtctc aataagtagg gctactacgc
     6901 ggtggggtag ctgctcgcct gaaaacaaca ttcgtctaag cctccacttg atgaggcttc
     6961 ctgaccattt agttgactac gtaataattc acgagctttg ccatacggtg gaaaaaaatc
     7021 atggcattaa cttctggaag ctgatgaatg agcattgtaa cggacacgcg cggcaacttg
     7081 ccagagagat aaaagaatac cacgcgtact aggtaatctt ttaatgaaag tggactagca
     7141 caaggatggt tagtaatcga gcgagtttaa cctttcggta agctctacta tttgggtgta
     7201 aaattcgctt gcaactttct gatagtcaat atttttccta cttccctttg caaaattttt
     7261 tctcgaaagc tttgaaacag tatcgcgctg cagtactatt acatcggaga tgatgtctaa
     7321 aatattttcc tggttaaact ccggtgagaa tgaaagctgg gtatagcatt cattcagaaa
     7381 tagcttaacc atgtgtttaa cttccttttt aaaatcacga atcttagaca tctcaccgta
     7441 aggttattaa cttcataata tttaactgaa taaatatata ctaaatttat atcatagtca
     7501 gctaaattga attattttat tcctattgtg cgtaaaatat taatgagcaa aataaaactt
     7561 tagaggaaaa caaattaaaa tagcttaaat atctacttta cagataagat aaaaggctag
     7621 tacccataag ctcttgcttc aattttttca ggcaatattt cgaaaacttt aacatttgca
     7681 agcgcaggag tgctatactt aaagttctgc agggaagtgt acttcagcat aaatatctcc
     7741 aaaacttcct ttttcttatc aatatcatca atttccttta ccataccgta gattaatacg
     7801 cttttgtaac gcatggagta tgagcatgca acactgtcat tttggtgatg catttggtag
     7861 tcggtgctaa actccacaca tacattcggg ttagatttcc aaatgccaat cttcttaccc
     7921 tcggggcctg aatggatgta aagcctatcg ttctgatagc caaaattgaa tggcagcaca
     7981 taaggcattc cgttggtatc aaccatgcca acatggcaca ccagcgagcg gtttatgata
     8041 tcattcactt cagccggggt taagttgctt ctcgatttca tgtctatcaa aaaaattagc
     8101 tatacaacgt tacaattatc tacatgattt acctgatata caaagcaaat aatggttaaa
     8161 aagtactgct tgtaaatgtt tcctaattat cctttgaagc tgttttaaag caaaaattta
     8221 taacgattga acattataca gcagtacatt ctaacacttt atcagaaaca ggttcagtac
     8281 aatacagtag ttcatagctt atcggacttt ttttaaagaa atccgcttaa aaaatctgta
     8341 cgcctcagga atccaaagca ctaaagagga ggccacaaat atttttaccc aatcggatag
     8401 gctaagcgga accgttctga aaatttctcc accccactga ataattacga cttgacctaa
     8461 aagtataaca aaagcaatgg taatgaatcc cttattatgg aacagtccct ttagagcaga
     8521 agagtaatcg ccaagcgacc tggagttgaa taggttccaa aattgaagca tcacaaagag
     8581 gttgaaaaag agggtaagct catagctgct aacgttgccg tcgcggccga agaagataag
     8641 catacctaac agaataatca gaaatgatgc agaagctagt agaatccctt taaaaattga
     8701 tggagttacg ataaattcct ttgggcttcg tggaggttta tttagtacgc ctgcatgggg
     8761 ttgctctgtg gccagggcaa gagcggcaaa ggtgtccata atgaggttca cccaaagcat
     8821 ttgggttact gttaaaggga gcgtaatccc ggtaaagggg ccggtgagca caattacaac
     8881 tgctaaaagg ttgattgtga gctggaagaa gaggaacttc tgaatattac cataaagggt
     8941 tctaccccac cgaatggcgt ttacaatgct ggaaaacgaa tcgtcaagca gaatgatatc
     9001 gcttgcctcc ttggcaactt gtgttcctga ccccatagcc aaccccacat cggctcggtt
     9061 aagcgagggc gcatcattag ttccatcacc tgtaacagcc actacctgcc cgttttcctg
     9121 tagcaacctg actagcctca ttttatccga aggacgtgcc ctgtacatta ttttaagctg
     9181 gtttgccatt ctgcgggctt catcttcgga aagtgcctca aaatcagggc ctgaaatgac
     9241 atttgcttcg gcattaccgg agagaatgtt tacttgcttc ccaattgatg ttgcggtaac
     9301 aatattatca cccgttagca tctttacatc aaccccggcc ctgtggcatt gggcaactgc
     9361 ttcagctgcg tcgaggcgaa caggatcggc aatacccaca accccaataa aattaagatt
     9421 ggatggcttt tcgttttcat tcacaacctc atggtctaga aacctataag cgaaagcgat
     9481 tgttctgagt gatttctgct ggtagctttc aagtaaaccc gtaagctttg cttgtaaaat
     9541 atcgtcaacc tcacacatgc ttattacaac ctcaggtgct ccctttacta gcaatattct
     9601 cttgtttaag ctgccggcct tcccaacggt gagcatgtat tttttttctg acgagaaagg
     9661 cagctgctgc tcaatggtaa aatcatttct aatgctctgg tagttaatac cttgattatt
     9721 aagccatagc aggagtgcag catcggttgg gttaccaata acagcgggag acttttcagt
     9781 aaaatcgaga tgagcagtag agtttaccgc cattgaatac ttaataagtt ctgcattaag
     9841 tgaattttcg gagatatcgt caacagcgga aaatgatgac tcaagaactg ccatacggtt
     9901 ctgggtaagc gtgccggttt tatcggtgca aacaactgtt acggcaccca tagtttcggt
     9961 ggcctgcagc ttccttacca gattgttgtc ggctgtcatc ttacgcatgc tgtaggctaa
    10021 actaagggta acgctcatgg caagcccctc aggaacagca actacaataa gagtcatgga
    10081 taccatgaaa taattcaaaa gcttactgat aaaatcaacc gaaaaccagt ttgcactgtc
    10141 tgccaggtct atgcctttgg caaatgcaat tggtatggca actaccacaa cagccaaccc
    10201 taaaagaaag gaaagcagat acgagttaat ctttgctttg gcaaatatat cagggattcc
    10261 ccttttggcc ccgaaggctt taactaagaa attaagggaa ggtagccaaa acttcatgag
    10321 cgctaccaca gctgcaacta caagcgatac aagaatgaat tgctggttag agctaagccc
    10381 gcttgtcccc ttagcaacat cagcaaccac caacagaaca aatatcagaa tggctaaaag
    10441 aaaggcaatt gagccaataa aatcgctaag ccggtttagc tgcttggtta ggggagtttg
    10501 aatatcaacc tgtagggttg attctctggc ggttttgcca atctcagtac tatcgccaac
    10561 tgatgttgtt tcgtaaattc catgcccttc aacaacagct gttcccttgt atacccggtt
    10621 aatcggatag gtgttatttc cttgatcaag tgaagaatca taacttttct gcgcaattgg
    10681 ttctcctgta agcgacgatt cgttcaccga aagggacacg gattcaagta aaacgccatc
    10741 gctgggtatc tcatcacccc tgtcaacaat aacaatatca cctactacta catctttctt
    10801 ggggatgctg gttactccgc cattccttat aaccttaacc gggttttcct cgtttacctg
    10861 gttcaaaata tcaaactcct tccctgccct atactcattg ataaacgaca taaatgtggc
    10921 cagaaaaatt gccacaataa tacctacacc ctcaaggtaa tgaccattaa cagccccaac
    10981 agcaatagct ataacagcgg caattgttag aatcctgata atgggatcgt taaatttttc
    11041 caaaagcaac cgccaccaag gtttacgcac tgggggagta agcacgtttt caccaaactt
    11101 agctctactc tcaactacct gctcctccga taagccttta tactttattt ccaccataat
    11161 tcaaaaattt ttggctaaaa ttagcatttc aatcaattct cataggttaa tattccttaa
    11221 aatttaaaaa taagcatttt tgttcaaaaa taattgaaca gccaatcccg aaatcaggat
    11281 gaaatatctt ggaaatcggg ttagagaaaa aattaaaaat taagaaaata ttttttgcct
    11341 aaaagtggag aattttcatc atgtaaacta aaaaatgtag gtttgcaaac tttttgatag
    11401 aaaacaaata gcaagtaatg ggaagagcat ttgagtaccg caaagcacgc aagctaaaac
    11461 ggtggggcaa catggccatt gtatttaccc ggttaggaaa ggagataaca atggtggcaa
    11521 aatcgggcgg cgcagatcct gacaccaacc ctaaactccg gttactgatt cagaacgcca
    11581 agtcggaaaa catgcccaag gataatattg aaagggccat taggcgtgca accgataagg
    11641 ataccaaaga atacaaggag gtcgtctatg agggctacgg accttatggg gttgccatcc
    11701 tagttgagac cgccacagac aatcctacac gaacggtagc gaatatccga agctacttca
    11761 acaagcatgg aggctcgctt ggaacttcag gtatgcttga cttcatgttt gagcataaat
    11821 gctcattcag aatagatggt aaagacagca tttcgctcga tgagctggag ctggagctta
    11881 ttgactacgg ggttgaggag gtgtttgttg aggacgatag tatccttata tttgccgact
    11941 ttgcacagtt tgggccggtt caacgttacc tggagggaaa agggctacat atcctaaact
    12001 ttcagtttga acgcattcca acagatacta agaagctaac tcccgaacaa caagaagaag
    12061 ttgagaaact ggtggataaa attgaggaag atgaagacgt tacccatgtt tttcataaca
    12121 tggaaattgc ttgacctgcc ctatttttct gaaaaaattc tacagctgca tttcgtatga
    12181 gtttcaggcc tggaaaagta aaacaggaac gttggactgg taaagcattt tcttggacaa
    12241 cgatgggtta aatagcttgt agataaggct tctcttcacg gatgttaatg ctaccaggtt
    12301 aattttctta tcataaatat agtcaataaa cgacttcacc atatccttac attcaacaat
    12361 agagtattca accgataatt caggatttac ctggtatagg taatttttaa gagacgacat
    12421 cagcgcctta atttcgacat tatcggggcg gtttgtcatg ttaacacagt gaaggttgaa
    12481 gttaaagggt gatagaatcc ttataagttt acgaattgaa ccaaaatcgc tttcgtccag
    12541 gttagtagca tacaggacat ttttaacctc atcaacaccc ttaaggacag ccttttcagg
    12601 aattgccaaa agaggtacct ttgtttcttc agaaacctcg gaggctacac taccgaccaa
    12661 ctcggtactt ttaaaacctt cgccctttgt acccatcaca atgatgccgg gtttgtacct
    12721 tttacttacc tctacaattc cataggctgc atatccctcc tgaagggtat atccaattga
    12781 aaccgaattg aaccccatct gtcgggcaaa ttcaaccaaa tcggcatgga atttaatgag
    12841 cttttccttg gcagctttgt aaagcaagtt gaagttaatg tcaaaatcta tctgaatacc
    12901 cgatacatcc gcaaaaggta tcatatcaac tacgggagag ttaaaaacat gcagcaattt
    12961 tatctcggca ttatactttt cggccagcat cacggcaaat cgggcagcat tttgcgaagg
    13021 ctcggagaaa tcgacaggta ccaaaatttt tcgtagcggt ttttccctct tttttgcctc
    13081 ctcgctaaac ttctgctcgg atagtagccg taaggctttt tcggcatcgg actcccggat
    13141 ctgaacctta accccttcgg aaacagctcc ctgcaatata ttctcgtttc ttaaaaagca
    13201 ctctatgccg ttagcttcaa ggtacgactg caacacaacg gctttagcgt aactttcaat
    13261 ggctatgact attagcttct cgtccatagg taaaaagttt tttgaaatat acactattaa
    13321 gccacaaaag tcaagaaaaa aagctacccc ctcccagggg cagcttctaa agaattcaac
    13381 atcaggatgc tactcttctt caagcaagct ctggataaag gctgaaattt gaggcattaa
    13441 ctcctcagcg ttaacctctg taccatctgc atagatgaat tttacggtaa gttcgccggt
    13501 ttcaaaattg cgtacaaaac tgaaatggcc aatttcccta tcggaggtag tataaagttt
    13561 tacgctgatg tactcattca ggtcaacatt tgggttggtc tcatagtcaa ggtcgtctgt
    13621 attaaaatcg gccgtagcag taaacttgag tgaattcata atcagtgttg cgtttaactt
    13681 gacgctggtg ttggtttcac taatcatgct tacattcagg ttcaagtcaa gggcgtaaag
    13741 ggttgtattc ccctgcttaa agctaatctg caacttggat agagagtata tttcggaact
    13801 gctatcggag taatcaatag aaacggataa cttgtaacca ccaaactcag cagttaaatc
    13861 gaaggattgg gttggaa
//
LOCUS       DAOSHM010000061        13547 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_3784_length_13547_cov_20.371035, whole genome shotgun
            sequence.
ACCESSION   DAOSHM010000061 DAOSHM010000000
VERSION     DAOSHM010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 13547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13547
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3784_length_13547_cov_20.371035"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            691..2211
                     /gene="rmuC"
                     /locus_tag="PK839_08725"
     CDS             691..2211
                     /gene="rmuC"
                     /locus_tag="PK839_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HOG72858.1"
                     /translation="MTGIVLVGILVVLAVVGISILLIRKPTVDIKPQLREVENLMVGF
                     GATLDRVEKSIKDEFQRNRAETNEISKTNREELTKSLDTFSSTFEKSVKELNDVLKAK
                     YDELRNQQADFNRQISDANKANREELANSLKSFETKFTENIRELNELLRQKFSDFSQQ
                     QTEVNRQLTENIKDVKQSVDNQLKSIREDNTHQLDEMRKTVDEKLQKTLNDRLSQSFE
                     TVGKQLQAVQVGLGEMKNLAADVGGLKRVLSNVKMRGGIGEIQLSMLLEQILAPDQYE
                     ANVRTKQNSADLVEFAIKLPGKDDSGKNVWLPIDAKFPKDIYEQLQDAYDAGDQNLIL
                     NAQKNLDSTIKKMAKDISDKYLDPPDTTDFGIMFLPFEGIYAEVVRKAALLEDLQRNY
                     KVIVTGPTTLAAILNSLQIGFKTLAIQKRSSEVWQVLGAVKREFENFGGLMEKAQKNI
                     QTGLNQLDDVMGKRTRAIQKKLKSVEALSEAEAKSILPEISESAIVDDEDDDLGEV"
     gene            2305..5193
                     /locus_tag="PK839_08730"
     CDS             2305..5193
                     /locus_tag="PK839_08730"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HOG72859.1"
                     /translation="MLKAKILMGSLLIAASLAGISQEVQMDNSRVKQLTLNNGLTVIL
                     DENHAQPKVFGLVMVKAGGKNDPADATGMAHYQEHMLFKGTQTLGTISWKEEKPYIDK
                     IFQLYDSLGKTSDEAKRKQIQQEINEASLKANSFAIPNEFSNLVNEIGGSMLNAGTGP
                     DYTVFYNMFPSSQIERWLDLYAHRFVEPVFRGFQAELEVVYEEKNLYNDQFFTKLLEE
                     FQKQFFKNHPYGQQTLIGSIEDLKNPSLTKMYDFFKTYYVPNNMALVLSGDFNSDEII
                     PVIEEKFGKWKEHELPESKIWTEKPFNGREFYQAKLSPVKMGLLGFRAPSAKDDGFLA
                     AEVATRILNNSYSTGLLDKLTLDGKLLAAQALQMPYLDHGAVIVVMLPKILGQSLEDA
                     ESLVNNELGKLKRGEFDDWMLEAVKLDLYRNHVTTMEKIDGRALYLGEAFAKGQSAQE
                     ALDYPNKIMRLTKDDVVAAANQIFGDSYLAFYSKMGFPKKEKIEKPDFKPLLANTEAK
                     SEYAKHFFNLPSKKPDFNPIVFDRDVETVEIGNGHKLMCVQNPANDIFSLTINFMIGE
                     NTMPLLRLASQGMGLSGAGSYNVTELKSEFARIGASYNITSNDYSVSIELQGVESELD
                     RALELVGMLVGDPRLEQSKIKTIVDGETTARKVERSQPDNVAQALLEYTLYGDSSNYL
                     NRLSSKQLKSLKAEELISTFKKATEYNTQIYYCGKTGASNVAAEVSKHVPLAANPKHD
                     TNSKDRLAKHYNENTIYLVDKPKARQSKVFVFINGQSFTPADAAVIDAFNDYFGGGFS
                     GLILQEIREYRSLAYSAGGKFKLPRQKGNPADFIGYVGTQSDKTLTAMETFNGLMRQM
                     PEKPERMGLIRSHLELLAQTDRPNFRELASRVDEWRDQGFEADPAFVKLPAYQLLKWQ
                     AIEQFYADRMKGKPAVYMIVGDKKQINLKELSSFGKVIELKEDKLFNK"
     gene            complement(5256..5329)
                     /locus_tag="PK839_08735"
     tRNA            complement(5256..5329)
                     /locus_tag="PK839_08735"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5293..5295),aa:Arg,seq:acg)
     gene            complement(5633..5998)
                     /gene="rplS"
                     /locus_tag="PK839_08740"
     CDS             complement(5633..5998)
                     /gene="rplS"
                     /locus_tag="PK839_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009162325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HOG72860.1"
                     /translation="MDLIKVAEESFASKKEHPSFKSGDTVVVHYKIVEGSKERIQNYR
                     GVVIQRRGVGATETFTVRKISGGVGVERIFPLNSPFIEKIEVAKEGKVRRARIYYLRN
                     LTGKKARIKEKKRKLDIGE"
     gene            complement(6369..8123)
                     /locus_tag="PK839_08745"
     CDS             complement(6369..8123)
                     /locus_tag="PK839_08745"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009138127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HOG72861.1"
                     /translation="MRKLAVQIFTLVSFAFLADTGWACTNFLVTRGASTDGSTFITYS
                     ADSHYLFGELYFRPAADYPAGTMMKIYEWDTGKYLGEIPQVGHTYSVVGNMNEHQVSI
                     GETTYGGRLELQDSTALIDYGSLMYIALQRSKSAREAIKVMAELVEQYGYYSEGESFS
                     VADKNEVWIFEMIGKGTNLKVDKKTKKSYNADKGAVWVAIRIPDGYISGHANQARITT
                     FPQENLKNSISSRNLDKIFDPGVEVVYSSDVIDFARTKGYYTGTNEDFSFSDTYAPLD
                     FGAARACEIRVWSFFKEVNQSMWDYFDYAKGHNLKHRMPLYIKPERKISVKDMMNFMR
                     DHLEGTELDMRKDPGAGPHAMPYRWRPMEWKYEGKEYVNERATATQQTGFSFVAQARN
                     WLPDPIGGIFWFSVDDVASTVYFPAYCGITAVPESYAEGKGDMLTYCDHCAFWTFNKV
                     SNFAYLRYDLMSQDIKKVQDELEGRFISNTQVVDATALKLYEQNQPEAIRFLTDYSVN
                     TGDYVVTRWEKLFQFLLLKFMDGNVKQVDENGQFIYNEYHQCPARVSNPEYPDWWKKA
                     VIDATGDKLLVPENKGGH"
     gene            complement(8223..9299)
                     /locus_tag="PK839_08750"
     CDS             complement(8223..9299)
                     /locus_tag="PK839_08750"
                     /inference="COORDINATES: protein motif:HMM:NF015647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HOG72862.1"
                     /translation="MVKLFNKNPLSVVGVMSGTSLDGLDICLAHFEKKSDSWAYTIEA
                     ANTVPYSAEVKIKLSDIEKLTAWEISKLDYDYGHFIGKEVFSFLKQINRHADVVASHG
                     HTAFHNPGMGYTFQIGKGAAIVAETGIPCISDFRAADICRNGQGAPLVPIGDKLLFTS
                     YDICLNLGGIANISFDNVDNERVACDVAPCNMLLNFLSLQLGMEYDAYGNAGAKGMVN
                     HDLLNRLNELEFYGLLGPKSLGREWFEQKVVPVFASCNASLQDKLRTAYEHIAIQISK
                     STAEISPGAMLVTGGGARNRFLVQLITEKVKHRVVVPENMLVDFKEALIFAFLAALFL
                     NGTPSALASSTGATADSISGCLYL"
     gene            complement(9299..10003)
                     /locus_tag="PK839_08755"
     CDS             complement(9299..10003)
                     /locus_tag="PK839_08755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72863.1"
                     /translation="MKRITAIMVVFLLAALGLADQAQAQRYRKKEISLISFKLYVDPD
                     YRAKLESYSNLFPEPQASSKVNKIDNLILYTSWDIIKRRLEGEVGMIILPIDAYGDKF
                     SYDMYGFPDISINKALSKGRSKYYIKLDLELVPEHSAKYTTQNHVDSARKIVQLKEDE
                     IKPKVVAKLTIYSDKGVIPVANCVGEATTKSIITLSSSFFDGVVNSSIDPSAETLYNL
                     IDKASQQLIISVYENK"
     gene            complement(10174..13398)
                     /locus_tag="PK839_08760"
     CDS             complement(10174..13398)
                     /locus_tag="PK839_08760"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOG72864.1"
                     /translation="MSRLVVYRASAGSGKTFRLAAEYIRMVVTNPYAYRIILAVTFTN
                     KATTEMKERILSELNQLAGGASTPMLEVIISESKLSETTIRQNAKSVLHAILHDYSMF
                     SVSTIDSFVQRVIQNLLWEVGFHSLGELMLEHDAILDRAVDRLLDSTNGDSELFTHLK
                     SMIFYQLDQGKSPDIKKSLVELGNLLYSEQYRLLTDEERAKMGDKSLLVNITTQATER
                     LNAIVQTICSMGKALMQEISSNGFTVDDFKNKSGGVYGIIEKAANFQLGLSYIEVGKR
                     AGDASRDNSVNQWMSMEFIKANPGKSSELQHLVQQSLQPGLARLVEYIEDNRSMFNTT
                     TALRSNLNRLYIVNEIRRYIKEIQAEDNVMVLADSGPLLREFIGENDAPFVYEKIGTR
                     YDSFMLDEFQDTSVIQWQNFKPLVGNSLAQDGFSMVVGDVKQAIYRWRNSDWRILASQ
                     LEEDFEVEPRNLSVNFRSLPGIVNFNNQFFGKAATWLKGWVNRLFEDNPPEDLLKVVE
                     KLYGGAAQESKFHEAIGGLVEVSCLQNIKAGRYDEFLKRKFSMLIPDILKRGYRPGDV
                     AILVRKHSEGKKIADMLLSLKNENPEWADSFNLVSQDALLLDSSHAIRLFISALKLIL
                     NTNDGVSRGVFCKESMVVAKISTSWEHTFVGSDIDDEIAWLTGLRNLPLSTIFEEVLL
                     RYNLQELKQELPYIALLNEQILNYSHGNLASVAGFLEWWDDRGHEVSLSLPDSADAIN
                     ILTFHKSKGLEFPVVIIPYASNSIFKNDKEKALWVSSATEPYCDYPKFLIGMADYLKD
                     SDFRDDYLAEKFQTMVDNLNLLYVAFTRPQNELYVMLSHQADEPKTVQDTGDIILSIV
                     KDMDAPEFTDDRFADISALAVYQFGLVAPIPKTSESKVENRPDVWLMEQYPVGKKIPS
                     LAGHLEASDFFKDEELTVTSGVEHGRIMHRLFSMIRGIGDVSMAVSRLVAEGLISLQQ
                     APALEQHVSEAIQNDAVADWFSEKWQELKVEASILTPTGQTYRPDRVMVSNGHAVVVD
                     FKFGEQQNAHKKQVAGYSELLSQMGYPNVTGYLWYFDSGSIVRV"
BASE COUNT         3834 a         3250 c         3037 g         3426 t
ORIGIN      
        1 ataaacgcta taatgtggtg tcttaccttt aaactcaaaa gtaatataaa atctcattta
       61 ataataaacc aaacacattt acttattaac aactctccag atgtgccgct ggctttagtt
      121 aaacttttgc ttacgtgggt ttcaactttt tgtggtcttt taaatcattg gcaatgaatg
      181 atttttgaat gcaccgcaaa tcggctaccg cttaaaccat cctggtggcg cagctgctac
      241 ctagacaggg caggatggcc gataatgaaa tgctaagatc cgcctaaaag tgagcggttt
      301 ttccagcagt caacaggatt gccacatgga aaggagtgtc catgcttcat gacattccaa
      361 atagccgcat gggtcgttat tattacacag caggtctatt cattattgtt tctatcgtct
      421 gattcaacct gttagcaggc tgaagcttgt ttgttaattt tcggcttaag ttaaaaccta
      481 agcctagcaa gggcataagc cgaacgatgg gcacccacct ctttcacatg cccggctgca
      541 aaattagctg ccacctaaaa agtaattcga tatttagaat acgcccaagg gcttattttt
      601 atacctttaa gccggcgata attgtggagg ttaccttaac aacctgctac gggtattcag
      661 gcatacatgt tgactttaaa aaattaagct atgacaggaa ttgttctggt tggaattctg
      721 gtggtgttgg ctgtggtggg catatccatt ctgctgatta ggaagcccac agtggatatt
      781 aagccccagc tgcgtgaggt tgaaaatttg atggtgggtt ttggcgccac cctcgaccgt
      841 gtagagaagt ccataaagga cgaatttcag cggaaccgcg cggaaaccaa cgaaatttca
      901 aaaaccaacc gggaggagct taccaaatcg ctcgatacct tcagcagcac ttttgaaaaa
      961 agcgttaagg agcttaacga cgtgcttaaa gccaagtatg acgaactacg gaaccagcag
     1021 gccgatttca acaggcagat ttccgatgcc aacaaggcca accgcgagga gctggcaaac
     1081 agccttaaat cgttcgagac aaaatttacc gagaacatcc gggagctgaa cgagctgctt
     1141 cgtcagaaat tcagcgactt ttcgcagcag cagaccgagg tgaaccgcca gctgacggaa
     1201 aacataaagg acgtgaagca atcggttgat aaccagctga aatccattcg tgaggacaac
     1261 acccaccagc tcgacgagat gcggaaaaca gttgatgaga agctgcagaa aacacttaac
     1321 gacaggctta gccagtcatt cgaaacggtt ggcaagcagc ttcaagccgt acaggtgggg
     1381 ctgggcgaaa tgaaaaacct tgctgccgat gtggggggac taaaacgggt gctaagcaac
     1441 gtgaaaatgc gtgggggaat cggtgaaatc cagctctcca tgctgctgga gcagattctt
     1501 gcacccgacc agtacgaagc caacgttaga accaagcaga acagcgcaga cctggtggaa
     1561 tttgccatta agctgcccgg aaaagacgac tccgggaaaa acgtatggct acctattgat
     1621 gccaagttcc ctaaggacat ctacgagcag cttcaggatg cctacgatgc cggcgaccag
     1681 aacctcattc tgaacgccca gaaaaatctg gacagcacca ttaaaaagat ggcaaaggat
     1741 atcagcgaca agtacctcga cccacccgat acaaccgatt ttggcatcat gttcctgcca
     1801 tttgaaggaa tctacgccga ggttgttcgc aaggccgccc tgctcgaaga ccttcaaaga
     1861 aactacaagg tgattgtaac cggacccacc accctggctg ccattctcaa cagcctgcag
     1921 attggcttca aaaccctggc cattcaaaag cgtagcagcg aggtttggca ggtgctaggc
     1981 gctgttaaga gggaatttga gaatttcggc gggctgatgg agaaagccca aaagaacatc
     2041 caaaccggtc tgaaccagct ggacgacgtg atgggcaagc gcacccgcgc catccagaaa
     2101 aaactgaaaa gtgtggaggc gctaagcgag gccgaagcca aaagcatact tcccgaaata
     2161 tccgaatcgg ctattgttga tgatgaggat gacgatttgg gtgaggtata gctaagctct
     2221 tatttaacct taattcgaaa aaaaccaaat aatccagggt gcaacattga acaacaacaa
     2281 tgcaacaacc taaaataaca agccatgcta aaagcaaaaa ttttgatggg aagcctgctg
     2341 attgcagctt ctctggctgg aatttcacag gaggttcaaa tggacaatag cagagtaaag
     2401 cagctcacgc tgaacaacgg tttaaccgta atacttgacg agaatcacgc tcaacccaag
     2461 gtttttggat tggttatggt aaaggcgggc ggtaagaatg accctgccga tgccaccgga
     2521 atggcacact accaggagca tatgctcttt aagggcaccc aaacccttgg caccatcagc
     2581 tggaaagagg aaaaacctta cattgataaa atttttcaac tctacgactc cctgggtaaa
     2641 acttccgatg aagctaagcg caagcaaatt cagcaggaaa taaacgaggc atcgctcaag
     2701 gcaaacagct tcgcaattcc caacgagttt agcaacctgg tgaacgaaat aggcggttcg
     2761 atgctcaatg ccggcaccgg ccccgactac accgttttct acaacatgtt cccttcaagc
     2821 cagattgaac gctggctcga cctttacgcc catcgttttg ttgaacctgt tttcaggggt
     2881 tttcaggcag agctggaggt ggtttacgag gaaaagaatc tatacaacga ccagtttttc
     2941 accaagctgc tggaggagtt ccaaaagcag ttctttaaga atcaccccta cggacagcaa
     3001 acgctcattg gctccattga ggatttgaag aacccctcgc tcaccaagat gtacgatttt
     3061 tttaaaacct actatgtccc caacaacatg gcacttgttc tctccggcga tttcaactcc
     3121 gacgagatta ttcccgttat cgaagaaaaa tttggcaaat ggaaggagca tgagctacca
     3181 gaaagtaaga tttggacaga aaaaccattt aacggaaggg agttctacca ggccaagctt
     3241 tccccggtga aaatggggct tctgggattc agagctccgt cggctaaaga cgatggtttc
     3301 ctggcagcag aggtggctac ccgcattctc aacaacagct actccaccgg attgctcgac
     3361 aagctaacgc tcgatggaaa gctgctggcc gctcaagctt tgcaaatgcc ctacctcgac
     3421 catggcgccg ttattgtggt tatgcttcca aagatacttg gccaaagcct tgaggatgct
     3481 gaatcactgg ttaataatga acttggtaaa cttaaacgag gcgaatttga cgactggatg
     3541 ctggaggctg ttaagctcga cctctaccgc aaccacgtta ccaccatgga gaaaattgat
     3601 gggcgtgcgc tctacctggg agaagctttt gccaaagggc aatccgctca ggaagcgctc
     3661 gactacccta ataaaataat gaggcttacc aaggatgatg tggttgctgc agctaaccaa
     3721 atcttcggag atagctatct ggccttctac tctaaaatgg gcttccccaa aaaggaaaaa
     3781 atagaaaaac cagactttaa acccctgttg gctaacaccg aagctaaaag cgagtacgct
     3841 aagcatttct tcaacctacc atccaaaaag cctgatttca accccattgt atttgatagg
     3901 gatgttgaaa cagttgaaat aggcaatggg cataagctga tgtgcgttca gaatcccgcc
     3961 aacgatattt tttccctaac aataaacttc atgatagggg aaaacaccat gcccctgctt
     4021 cgcctggcct cacagggaat gggccttagc ggcgccggca gctataacgt aaccgagttg
     4081 aaatcggagt ttgcccgaat tggcgcatcg tacaacatca cctcaaatga ttacagcgtg
     4141 tctattgaac tacagggagt ggaaagtgaa cttgaccgag cgctcgagct tgtaggaatg
     4201 ctggtaggcg acccccgcct tgagcagtcg aaaattaaaa caatagttga cggggagact
     4261 accgctcgaa aagttgaacg ttcacagccc gacaatgtag ctcaggcgct actggagtac
     4321 accctttatg gcgatagttc aaactaccta aaccgcctat cgtcgaagca gctaaagagc
     4381 ctgaaagccg aggagctgat ttcaaccttt aaaaaagcaa cggagtacaa cactcaaatt
     4441 tactactgcg gaaagacagg cgcatcaaat gtagccgcag aggtgagcaa gcatgtccca
     4501 ttggctgcca acccaaaaca cgataccaac tcaaaggaca ggctggccaa gcattacaat
     4561 gaaaacacca tctacctggt tgataagcca aaggccagac aaagcaaggt gtttgtattt
     4621 atcaacggcc aaagctttac acctgcagat gcagctgtaa tagatgcttt taacgactac
     4681 ttcggaggag ggttctctgg acttattctt caggaaataa gggaataccg ctcgttggcc
     4741 tactctgccg gaggaaaatt taagttgccc cgccaaaaag gaaatccagc tgattttata
     4801 ggctacgttg gaacacaatc cgataaaacc ctgacggcca tggaaacatt caacgggctg
     4861 atgcgccaga tgcccgaaaa gcccgaacgt atgggcctta taagaagcca cctcgaactt
     4921 ttggcgcaaa ccgaccgacc aaacttcagg gagctggctt caagggttga cgagtggcgt
     4981 gaccagggtt tcgaggccga ccctgccttt gttaagctac ctgcctacca gctgcttaaa
     5041 tggcaggcta tagagcaatt ctatgccgac aggatgaagg gtaagccggc tgtttacatg
     5101 attgttggcg acaaaaagca aatcaacctt aaggagctgt catcgtttgg aaaagtgatt
     5161 gaactcaagg aggataagct gttcaacaaa taactttggg agagattact gaaaagtaaa
     5221 aaaagaccaa tacgcgtatt ggtctttttt tgttgtgacc ccgaagggat tcaaaccccc
     5281 aaccttctga tccgtagtca gatgctctat tcagttaagc tacggggcct tgtgtttcaa
     5341 gtgcaaaatt attcaatatt tccataccat gcaaccattt cctgctgaaa aaaaataaaa
     5401 aaccgtcatt tcaaccagta aaatttactc cttaccaaag cgttaaccgg ctgatttaac
     5461 tacaaatcag gcaattatca aaacaataaa ggcaaccact tgcaaacgcc tgcctacaac
     5521 aaattgcgca ttaaacccaa actgatattc agaagggata tgggagctat taaattggtt
     5581 taagcatggg tggttaataa aaaatcccca acttaaaggg gattttatca gcttactctc
     5641 caatatcgag cttgcgtttt ttctccttaa tgcgtgcctt tttacccgtg aggttgcgca
     5701 ggtagtatat tcgggctctg cgaacttttc cctccttagc cacttcaatt ttttcgataa
     5761 atggggagtt aagcggaaaa atcctttcaa cacctacacc acctgaaatc tttcttacgg
     5821 taaaggtttc ggttgccccc acgcctcttc tctgaattac gacgcccctg taattctgaa
     5881 ttcgctcctt gctaccctca acaattttgt agtgaactac aacggtatca ccactcttaa
     5941 aggaaggatg ctccttttta gaggcaaatg actcttctgc aactttaatg aggtccattt
     6001 tagtttgatt tttaagtata actgggaaat gcaatattac cggcacccat gtcctggtaa
     6061 gagattacac ttcaaattgg gcgcaaattt agaaaattaa tattaataaa caggtaaatt
     6121 aaagccaata atttgatttt tttcatgaac catatgttga aacacccctg agtaggcaaa
     6181 ccgctagcgg ggatgataag gggagtaaac gcttgccctc aacgatttca accaggctca
     6241 tccgaaagat agccaacatc aaaaacatct aacgcgacct ttttgtcaac cggtaaaaat
     6301 gtgcatttga ggggaaagaa aatgcggagg aaaaattttc ctccgcatag ctaggtggct
     6361 caataatact agtgcccccc tttgttttca ggtacaagaa gtttatcgcc ggtagcatca
     6421 attactgctt tcttccacca gtcggggtat tcaggattgc ttacccgtgc tggacactgg
     6481 tggtactcgt tgtaaataaa ctgaccattc tcgtcaacct gcttcacatt cccatccatg
     6541 aacttcagca gcaggaactg gaaaagcttt tcccaacgcg taacaacgta gtcgccagta
     6601 ttaacggagt agtcggtaag gaacctgatt gcctcgggtt ggttttgctc gtagagctta
     6661 agagcggtag catctacaac ctgggtgttg ctaataaacc tgccctccag ctcgtcctgc
     6721 acctttttaa tatcctggct catcaggtcg tacctaaggt aggcgaagtt ggaaacctta
     6781 ttaaaagtcc agaaagcaca atggtcgcag taggtgagca tgtcgccctt accctcggcg
     6841 tacgattcgg gaacagctgt tataccgcag tatgccggga aataaacggt tgaggccaca
     6901 tcgtcaacac tgaaccagaa aataccgcca attgggtcgg gcagccagtt ccgagcctgt
     6961 gcaacaaacg agaagccagt ttgctgggtg gcggttgcgc gttcgttaac gtactcctta
     7021 ccctcgtatt tccactccat aggacgccag cgatatggca tggcatgagg gccagctcca
     7081 ggatccttcc gcatgtcgag ctccgttccc tccaggtggt cgcgcatgaa gttcatcatg
     7141 tccttaacgg aaatcttcct ttcgggcttg atgtagagcg gcatacgatg ttttaggtta
     7201 tgccctttgg catagtcgaa gtaatcccac atggactggt ttacctcctt aaaaaacgac
     7261 catacccgaa tctcgcaagc acgggcagca ccaaaatcga gcggagcata ggtatcggaa
     7321 aagctaaaat cctcatttgt gccggtatag tatcccttgg tacgggcgaa gtcaatgaca
     7381 tcagaagagt aaaccacctc aacacccgga tcgaaaatct tgtcaaggtt cctggagctt
     7441 atagaatttt tcagattttc ttgagggaag gtagtgatgc gggcctggtt ggcatgtccc
     7501 gagatgtagc cgtcgggaat acgaatggct acccaaacag ctcccttatc ggcattgtag
     7561 gatttctttg ttttcttgtc aaccttcagg ttagtcccct ttccaatcat ttcaaaaatc
     7621 catacctcat ttttatcagc cacagagaac gattccccct cgctgtagta cccgtactgc
     7681 tccaccagct ctgccataac cttgatggct tcacgagcgc tcttggaacg ttgcagggca
     7741 atgtacatca gcgatccata gtcgattaat gctgtgctgt cctgaagctc caggcgcccc
     7801 ccgtaggtgg tttcaccaat ggatacctga tgctcgttca tgtttcctac caccgagtag
     7861 gtgtggccaa cctggggaat ttcacccaaa tacttaccgg tgtcccactc gtatattttc
     7921 atcattgtgc ctgcagggta atcggccgca ggacggaagt aaagctcgcc aaacaggtag
     7981 tgggaatcgg cagagtaggt aataaaggtt gacccgtcgg ttgaggcacc cctggttacc
     8041 aaaaagttag tgcacgccca ccccgtatca gcaagaaaag caaaggatac cagagtaaaa
     8101 atttgaacag caagttttct catagcttgt cattttaaga attagcaaat tgcaaaccta
     8161 gcatttattt cgatttttct gcatgatgaa ggtttaatca accatattct tcaccatcag
     8221 aattataggt atagacatcc ggaaatggaa tcggcagtgg caccggttga tgaggcaagc
     8281 gccgaagggg taccgttaag gaaaagggct gcaagaaagg caaaaatcaa agcttcctta
     8341 aaatcaacca gcatgttttc tggaactacc acgcgatgtt tcactttttc tgtaatgagc
     8401 tgcacaagaa atctattcct ggcgccaccg ccggtaacca gcatagcacc tggactaatt
     8461 tctgctgttg atttagaaat ctgaatggca atatgctcgt aggctgtacg tagcttatcc
     8521 tgaagggatg cattgcagct tgcaaaaaca ggaactacct tttgctcaaa ccactcccta
     8581 ccgagtgatt tcggcccgag caacccgtaa aactccagct cattaagccg gttgagcagg
     8641 tcatggttaa ccatcccttt tgcaccagca ttgccataag catcatactc cattcccagc
     8701 tggagcgaaa ggaagttgag cagcatgttg catggtgcaa cgtcgcaggc caccctttcg
     8761 ttgtcaacat tgtcgaagct tatgttggct attcctccaa ggttcaggca gatgtcataa
     8821 ctggtgaaaa gcagcttatc accaatggga acaaggggtg caccctgtcc atttcgacaa
     8881 atgtcagcag cgcgaaaatc ggaaatgcac ggaatgcccg tttctgcaac tatagcagca
     8941 cccttgccaa tttgaaaggt atagcccatg ccaggattgt gaaaagcagt atgcccatgc
     9001 gaggcaacca catcggcatg gcggtttatt tgcttcagga aactaaaaac ctccttccca
     9061 ataaagtggc cataatcgta atcgagcttt gatatttccc acgctgtcag cttttcaata
     9121 tcacttagct tgatttttac ttcagcacta taagggacag tattggctgc ttcaatggtg
     9181 taggcccacg aatctgactt tttttcgaaa tgagctaggc aaatatccaa accatcaagc
     9241 gaggttccag acattacacc aactacgctc aacggatttt tgttgaagag ctttaccatc
     9301 acttgttctc gtaaacgctg atgatcagct gctgcgaggc cttatcaatc aggttgtaaa
     9361 gcgtttcagc agaaggatcg atgctgctgt tcaccacgcc gtcgaaaaac gagctgctaa
     9421 gggtaataat gctttttgtt gtagcctccc caacacaatt tgccaccggt ataactccct
     9481 tatcggagta tatggtaagc tttgcaacta cttttggttt aatctcatcc tctttaagct
     9541 gaacgatttt cctagcagaa tcaacgtgat tttgggtggt gtacttagca gaatgctccg
     9601 gaactaattc caaatctagc ttaatatagt actttgatct tccctttgaa agggctttat
     9661 ttatactaat atccggaaat ccgtacatat cgtaggaaaa cttatcacca taggcatcta
     9721 tgggaagtat aatcatacca acctcaccct cgagccttct ttttattata tcccacgaag
     9781 tgtaaagtat aaggttgtca atcttgttta ccttactgct agcctgaggc tcggggaaca
     9841 ggttgctata actttcaagt tttgcccgat agtcggggtc aacgtatagc ttgaatgaga
     9901 ttagcgaaat ttcctttttg cggtatcgct gcgcttgcgc ctggtcagcc aaacctagag
     9961 ctgccagtaa aaacacgacc attatagctg taattctttt catcccccaa tcaatttata
    10021 aattccggaa aaaactctac gagcaaagat agtcaatttt tcaaacgagc tccgggttca
    10081 ataaattgta cttttgctac aaatttcacg tttgtagcgg ggttctcaat tgcttcagca
    10141 taaaagtggc agtgagctgc cagcagttag cttttacacc cggactatgg aaccggaatc
    10201 gaagtaccaa aggtaacctg tcacattggg gtaacccatt tgcgacagga gttccgaata
    10261 ccctgccacc tgcttcttgt gggcattctg ctgctcgcca aatttaaagt caactaccac
    10321 ggcatggcca ttgctaacca tcacccggtc ggggcgatag gtttgcccgg ttggggtgag
    10381 gatggaagcc tcaactttca actcttgcca tttttctgaa aaccagtcag ctacagcatc
    10441 attctgaata gcttcgctga catgctgttc caacgcagga gcttgctgga gagaaatcag
    10501 gccttctgca acaagtcggc tgacggccat gctcacatca cctattcccc tgatcattga
    10561 aaacaggcgg tgcataattc tcccgtgctc tactcctgag gtaacggtta attcttcatc
    10621 cttaaaaaaa tcggatgcct caaggtgtcc tgccagggaa ggaatttttt ttccaacagg
    10681 gtactgctcc atcagccaaa catcggggcg attctctacc ttactctcgg atgtttttgg
    10741 tattggagca accagcccaa actggtaaac agctaatgct gaaatatcgg caaacctgtc
    10801 atcggtaaat tcaggggcat ccatatcttt aacaatggaa agaatgatgt ctccggtatc
    10861 ctgcaccgtt ttgggttcat cagcctggtg gctaagcatg acgtaaagtt cgttctgagg
    10921 tcttgtaaag gctacgtaga gcaggtttag gttatccacc atggtttgaa acttctcggc
    10981 aaggtagtcg tctctgaaat cgctatcctt aaggtagtcg gccatgccta tcaggaattt
    11041 cgggtaatcg cagtatggtt cagtagcaga cgacacccat aatgcttttt ccttgtcgtt
    11101 cttaaagatg ctattgctgg cgtaggggat aatcacaacc ggaaactcaa gccccttgga
    11161 cttatgaaaa gttaaaatgt tgattgcatc agccgagtcg ggaagcgaga gggaaacctc
    11221 gtgtccacgg tcatcccacc actccaggaa gccagcaact gaagccaagt tcccgtgaga
    11281 gtagttcaaa atctgctcgt tgagcagggc tatatagggc aactcctgct tcaactcctg
    11341 caagttatag cgaagtaaaa cctcctcaaa aatggttgaa agtggaagat ttctaaggcc
    11401 agtaagccag gcaatctcgt catcaatatc gcttccaacg aaggtatgct cccagctggt
    11461 tgaaatttta gctaccacca tgctctcctt gcagaaaacg ccccttgaca cgccatcgtt
    11521 ggtgtttaat ataagcttaa gggccgaaat aaagagccta atagcatgtg atgagtcgag
    11581 tagcagggca tcctggctta ccaggttaaa ggagtccgcc cattcaggat tttcgttttt
    11641 gagcgatagc aacatgtcgg caatcttttt gccctcgctg tgtttcctaa ccaggatggc
    11701 tacatctccg ggtctgtacc cacgtttcaa gatgtcgggt atcagcattg agaacttgcg
    11761 ttttagaaac tcatcatacc tacctgcttt aatattctgt aagcacgata cctccacaag
    11821 tccacctatg gcttcatgaa atttgctttc ctgtgctgca cctccataca gcttttcgac
    11881 tactttcaaa aggtcttcgg gaggattatc ctcaaagagc cgattaaccc aacccttcaa
    11941 ccatgttgca gctttaccaa agaattggtt gttaaagttc acaatcccgg gaagactgcg
    12001 aaagtttacg gacaaattgc ggggttccac ctcaaagtct tcctcaagct gcgaggccag
    12061 tattcgccaa tcgctgttac gccagcggta aattgcctgc ttcacatcgc caaccaccat
    12121 gctaaaacca tcctgagcca agctattgcc aaccagaggt ttaaagtttt gccactgaat
    12181 aaccgaggta tcctgaaact catcgagcat aaagctatcg tagcgggtac cgattttctc
    12241 ataaacaaac ggtgcgtcgt tctccccaat aaactcacga agcaatggtc cgctatcggc
    12301 tagcaccatg acgttatcct ctgcctgaat ctccttgatg taccttcgta tctcattgac
    12361 tatgtagagc ctattcagat tggaacgtag cgccgtggtt gtgttgaaca ttgagcggtt
    12421 gtcctcaatg tactcaacca gcctggccaa cccgggctgt aacgattgtt gaactaagtg
    12481 ctgcaactcc gatgatttgc ctggatttgc cttgatgaac tccatgctca tccattgatt
    12541 tacggagtta tcccgtgagg catctccggc ccttttacca acctcaatgt agctcaaccc
    12601 tagttgaaaa ttcgcagctt tttcaatgat tccataaacg ccgcccgact tattcttaaa
    12661 atcatcaaca gtaaagccgt tgctgctgat ctcctgcatc aaggccttgc ccatgctgca
    12721 aattgtttga actatggcat ttaacctctc cgtggcctga gtggtaatgt tgaccagcaa
    12781 cgatttatcg cccattttgg ctctttcctc gtcggtaagc aaccggtact gctcggagta
    12841 aagcaggttg cccagctcta ccaaactttt cttaatatca gggctttttc cctgatcgag
    12901 ctgatagaag atcatggatt ttaagtgagt aaatagctct gaatctccgt tagtgctgtc
    12961 gagcagcctg tcaacagccc tgtcgagaat agcatcatgc tcaagcatga gctcgcccaa
    13021 gctatgaaag cctacctccc agagcaggtt ttgaatgaca cgctgaacaa agctgtcgat
    13081 ggtgctaacg ctgaacatgg agtagtcgtg gaggatagca tgcagaaccg atttggcatt
    13141 ttggcgtatg gtggtttcgg acaatttaga ttctgaaata ataacctcga gcatcggggt
    13201 gctggcacca ccagccagct ggttcaactc cgaaagtatg cgctccttca tttcggtggt
    13261 tgccttgttg gtaaatgtta ccgctagtat aatcctgtag gcataagggt tagttaccac
    13321 catgcggatg tactcggcag ccagcctgaa agtctttcca gaaccagcgg atgccctata
    13381 aacaacaagc ctactcatag caacagctaa aaatcaatca actctaaaaa taggaaaaat
    13441 ctgaataaat ccacagggtg ggcagaatgt tgctggaatt ataagtgggt gttaaaggat
    13501 aaaggttaaa ggataaaaaa attcaagcag gattcaagaa cctgtcc
//
LOCUS       DAOSHM010000062        13139 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_3965_length_13139_cov_21.233491, whole genome shotgun
            sequence.
ACCESSION   DAOSHM010000062 DAOSHM010000000
VERSION     DAOSHM010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 13139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13139
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3965_length_13139_cov_21.233491"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            100..657
                     /locus_tag="PK839_08765"
     CDS             100..657
                     /locus_tag="PK839_08765"
                     /inference="COORDINATES: protein motif:HMM:NF014689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HOG72865.1"
                     /translation="MSVFTLILLALGLSFDTLAVSISSGIARKQIVFFEAFRLAAVLG
                     FFQAAMPILGWLGGISIKKYIEPVDHWVALALLVIIGVKMIIESFKDDGVKTFNPLEP
                     RVMVGMALATSIDALAVGVSFAITQVNLILAFFVFGSITFLVAMLGMLFGKKIGNHIG
                     HRMEIIGGLILIGIGVKIVLEHLNG"
     gene            complement(660..1355)
                     /locus_tag="PK839_08770"
     CDS             complement(660..1355)
                     /locus_tag="PK839_08770"
                     /inference="COORDINATES: protein motif:HMM:NF024587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HOG72866.1"
                     /translation="MSYTVLKYLVEKNRQPNNTQKEPGPVVTISRQMGCPGNRVTNLL
                     VQELNQRYCLKGESQWNGLAKEKIEQIAAERLGLPKEEIDYVFEAKRKGAMEEILHSM
                     STKYYKSDQRIQNTVKGVIRMEAEKGRVVILGRGGVAITRDIPRSLHIQVEAPFEWRV
                     LRVEEMYHFDNKSAQLYVNEIDKKREAMRVYFGGKGTDYTRFDLTFNAMTLSVKEIVD
                     IIIYTMEVRKLID"
     gene            complement(2024..2296)
                     /locus_tag="PK839_08775"
     CDS             complement(2024..2296)
                     /locus_tag="PK839_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET transporter"
                     /protein_id="HOG72867.1"
                     /translation="MVAQIIGLLAAILSTLNQFPQAYKVMKTKDTQSISLYMYCIVEV
                     AIALWLVYGIMLRDFPLILANSLSLIPITYIFVVKLLHVISGKESI"
     gene            complement(2314..3048)
                     /locus_tag="PK839_08780"
     CDS             complement(2314..3048)
                     /locus_tag="PK839_08780"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOG72868.1"
                     /translation="MDDELRLIPNLSIDCVIFGWDEGVLKVLLRKELIEYKDIVHIEW
                     KLPGNHVGMNERIEDTATRVLKEQAGVENIFMKQFMVFSDLDRLRRREMDYQWIKKYG
                     LGEDRVITVGFYSLVNLLNFDSSKLLNVAEWHNVDEVESLIFDHKEILGEALKKLKED
                     VTMHPIVFELLPKKFTLTQLQQIYEVILGTRFDKRNFRRKIANLKYVVSCNEKQYGVG
                     HKPPSLFRFDPKIYSKIHQKNLDATA"
     gene            3276..5585
                     /locus_tag="PK839_08785"
     CDS             3276..5585
                     /locus_tag="PK839_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HOG72869.1"
                     /translation="MYRIRLWPLGIAVIALIFGSCNVEKRSDAEQVAKAMLDRMTLEE
                     KVGQMAQITLDVVGKGDSLFNSYEPFELDTAELREAIVDYHVGSVLNSSNNRARSLAA
                     WNSIVSTIQNVAIAKTRLAIPVLYGIDAVHGANYTAEATLFPQQIGMAATWNDSLVQA
                     IGSITAYETRASGIPWVFSPVLDLGADPRFPRQYEGFGEDPHLCSVLGVALVNGYQGA
                     SVSSPLKVAACAKHYLGYSAPSSGKDRTPAQLPNITLKEYHLPPFKAAVEAGVKTFMV
                     NSGVINGLPVHASHYIITELLKEELGFEGVVVSDWNDIENLYRRDRVVATEKEAIGLA
                     VNAGIDMAMIPYRYQDFCNQLVELVREGSVEESRIDDAVMRILKLKAELGLFERPTTV
                     LGDYPKAGSEEFAKVSYQAAGESITLLKNSNGILPLSKDKRYKILVAGPNANSMRTLD
                     GGWSYSWQGEKVEEFAGRYNTIFEAIKSKVPQSEVSYIPGVSYPAYGRYFEEQYDKFE
                     ESAAAARKADVIILCLGENSYAEKPGDLNDLYLGSKQLNLAEELAKTGKPIILVLNEG
                     RPRVISPIEPMMAAVVQTYLPGSYGADALADILFGDINPSGRLPYTYPSYPNSTVVYY
                     HKPAEEQKPNIGAYSYESDYNPQYPFGYGLSYTNFEYSQLTVNRSELKQNDTLTISVN
                     VRNTGDRKGKDVVQLYTSDLVATITPDVKRLRKFAKIELAPGEAKTLTFKLVTSELAF
                     VDSNDRLVVEPGEFIVSVGNLSTKFHIVN"
     gene            5611..8586
                     /locus_tag="PK839_08790"
     CDS             5611..8586
                     /locus_tag="PK839_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018074694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOG72870.1"
                     /translation="MKYFLKIFRRNARILLLGLLCVCWIPSMAQTITVSGRVTDKEGA
                     PMAGVNIVVKGTTNGTNTDVDGKYSISTPANAILQFSFIGMKSVEEAVGGRSIIDVVL
                     ESESMDISEVVVIGYGTSRKKDITTAVSVVSTEDFDKRPVTNVAQAIQGKAAGVQVVL
                     PSGKPGGDAFIRVRGATSVLAGNDPLFVIDGVPTTSMQALNPQDIETVQVLKDASSAA
                     IYGARAANGVVIITTKHAKAGVASVSVNSYMGFSNLANSLDVLNTEQYRELMDEVKGP
                     GTVPTSQTNYTNWIDETFSTGQIQNYQVSVSNGTEKMKYYFSAGFSDEKGIVKPAENK
                     KYTIRTNINSDLFKWLKFGTNISFIHNWYNDVSDNANSGRAGTILSAINTPPFLHIWD
                     PNNPEQYEVNPFQNSWENPLAYASRINQSKTERVLANANTEITFLKDFKLKSNFGIDG
                     YIYQRDLYIDPVKTVYGRTENGIGEADKSNSLVWLIENVLSYEKTFGNHNLSVQAGQS
                     AQSSRWDQSYISAKDFPKNSAVTTINAANQITYAASSASEWAIASFFGRVSYNYMSKY
                     LFTANFRYDGSSKLSSDNRWDFFPSFSAAWRLSSEPFLKNVVFINDLKIRGGWGRNGN
                     QEGIGDYAWQALYGFSRQSPTSPLSGPGIYQMNLENPSLKWEVTYQSNVGLDLTVLDS
                     HLSFTGDVYWKNTDGLLVNVPLPSGSAAVDYITRNDGKMKNWGVELSLDAKDFRHELK
                     WSTNLFWSMNRNEITELQLSQIYNFAQLESNNEYVIRMSPGYSLGTFYGYVAKGVDPE
                     TGDMIYEDRNNNGVIDPDDRTVIGSAEPDFIYGLNQTLTYKGIGLTLFFQGSQGNDIF
                     NASRIDTEGMFDVKNQTTEVLRRWKRPGQITDIPRAVSNGSTFNVHNSTRFVEDGSYF
                     RLKALTLSYSLPSKWLSRVSIKNFTLYATATNLFTITGYNGFDPEVNYGGNNSVIQGV
                     DYGTYPQSRSYIFGLSFDF"
     gene            8599..10080
                     /locus_tag="PK839_08795"
     CDS             8599..10080
                     /locus_tag="PK839_08795"
                     /inference="COORDINATES: protein motif:HMM:NF025680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOG72871.1"
                     /translation="MKKLNGYLLAGVALLTFTVVSCEDFLDKDPISNYSNSTLGVDNN
                     TDTLKYTTAEQAEALLATCYSNFRGELFQLDYFVNGDMQSDDAYAGASGTEFSQFDNF
                     SIDATNKNVERDWKYLYKMVTDCNTVIVNVPKITDQALTDARKKEIVAEASFIRAWAY
                     FDLVRLYGDVPLVLDVVTTITADNVAEVYPLLYPKRDSRELVYDQIITDLNTALDGVP
                     VTAPNKGYVTKGAVNALLARVYATRQPIDYTKVNDYCDAVINGGYSLLSNYDYLWDFQ
                     HENSSEAIFEIQYDGNWNTGTGNWAVSMFLGTGWKKFNTPSNDLVAAFDAENDVIRKN
                     SSIKFEDVTGSWTDANWPTSTYPFIYKIRDEASTSNIILMRLADFILLKAEALNELGL
                     PGADTLVNQIRGRVNLPSVSPTTKEQMRLIIEKERRLELAFEGIRWFDLLRTGRAITV
                     MENVRGGNGELLYAGKLQPYKLLWPIPQEQRDLNSMLTQNPGY"
     gene            10101..11453
                     /locus_tag="PK839_08800"
     CDS             10101..11453
                     /locus_tag="PK839_08800"
                     /inference="COORDINATES: protein motif:HMM:NF027723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5016 domain-containing protein"
                     /protein_id="HOG72872.1"
                     /translation="MKILKYTCIVGLALVAFTACEDNETKTTSQPTISNVSISPTAGL
                     TYGDSIAVSASVSDEVTPLSTLDIQVIVADVEIYKKSIRTKGNSANVSAKFKLPFAAN
                     AEDNGEVTVKLQSINIDGFSTSQTETLHAARPILGDILYLVTSSDTIELAKSPENPFL
                     YLSPTGSYPNEVVGKIATSENLEAAEWVWVSSGSAIVTGSYTDSDIRLSDPSYQVEQL
                     SFNVMTFELKFIGELLPSVKFNTTTLLMGEDGRFHGEVEFTKGDVVTVEGISNLAAAY
                     NPDFFDYKDGVLTFKAPTGKYQVVYWAGTINYMWLRQDNAVYPDALWLTGAGIGMPTF
                     INDVTNIWWGWDDWGVTTRQSYFFCPKIGENKYQVTVYLMNTEGSWWLQTEFKLFPTK
                     SWDNEFSSMDWGYISSNLSPNTNGNFVHTPELTEGLYRITIDMSGGKGNYFLIAQKIN
                     "
     gene            11623..13089
                     /locus_tag="PK839_08805"
     CDS             11623..13089
                     /locus_tag="PK839_08805"
                     /inference="COORDINATES: protein motif:HMM:NF014147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 30 beta sandwich
                     domain-containing protein"
                     /protein_id="HOG72873.1"
                     /translation="MKNIHNSLFSFASGMLSACLIFFAILLPGCTDDSDSVDNPPDDT
                     TQLGDVRVWVTTCNQAYLFKEQSVSFSDDVAPFTIRVDTLNRYQQVEGFGAALTGSSA
                     YNLRRMTDSQRTSLLTELFDPVKGIGLSYLRLSIGSSDFSLSLYTYCDYPSIDSFALH
                     PTDLRDLIPVLKEILAINPAIKIMATPWTPPIWMKTSAAWSGGSLIPDFYDEYAEYFV
                     RYIRAMAGEGITIDAISVQNEPMWEFGTPGMKMTWQEQLTFIRDFLGPKFAAEDISTK
                     ILIWDHNWDMPDYPISILSDPAAYMYIAGSAFHGYGGEYSAMLSVHNQFPQKGLYFTE
                     ISGGGWATDFDDNLKWTIENVFFGTLSCFSKNVLLWNLVLDDNWGPFIQGGCSNCRGV
                     ITLNSDGSLTRFNEYYALAHFAKAIRPNAYMLGTTVVGDMSQLADFKYVAAQNPDGSK
                     VVVAMNFSSVGKKLAVRCGSNKFTFTVQPESLVTFMWR"
BASE COUNT         3444 a         3023 c         3075 g         3597 t
ORIGIN      
        1 atataatgtt gatataaaag caaaagcgct caaatcaaaa taatgggttt gctgtacggt
       61 attggttgtt atgtttaact ttgccgccaa aagcaattca tgtcagtatt cacccttatt
      121 ctcctggcct tgggcctttc gttcgatacc ctggctgttt ccatttccag tggaattgca
      181 cgtaagcaga tagtattttt tgaagctttc cggttggctg ctgtgctggg attcttccag
      241 gcagccatgc cgattttggg ttggcttggc ggaatatcca taaagaagta cattgaaccg
      301 gttgaccact gggtggcgct cgcccttttg gtcattatag gcgttaaaat gatcattgag
      361 agctttaagg atgatggggt aaagacattt aacccgctag aaccaagggt gatggtagga
      421 atggcgttag ccaccagcat agatgcgcta gccgttggag taagctttgc aataacgcag
      481 gtgaacctta ttctggcatt tttcgtcttt ggctccatta ccttcctggt agccatgctg
      541 ggaatgctgt ttgggaagaa aattggcaac cacattgggc accgtatgga gatcatagga
      601 gggctaatac ttatagggat aggggtaaag attgtgctcg agcacctgaa tggctagcgc
      661 taatcaatca gcttacgcac ctccatggtg tagatgatga tatcaacaat ttccttaacg
      721 gagagggtca tagcgttaaa cgttaggtcg aaccgggtgt agtcggttcc tttcccgcca
      781 aagtaaaccc gcatggcctc gcgctttttg tctatttcgt tcacgtagag ctgggctgac
      841 ttattgtcaa aatggtacat ttcctcaact ctaagcactc tccattcaaa gggtgcctca
      901 acctggatat gtaacgagcg gggaatatcg cgggtaatgg ccactccacc tcttcccagt
      961 atcaccactc gtcctttctc cgcctccatc cggattacac cttttaccgt gttttgaatc
     1021 cgctggtcgc tcttgtagta tttagttgac atggagtgga gtatttcctc catagctcct
     1081 tttcgtttgg cctcaaacac gtagtcgatt tcctcctttg gcaaacctaa tctttcagca
     1141 gcaatttgtt caattttctc tttcgccagc ccgttccact ggctttctcc tttaaggcag
     1201 tagcgctggt taagctcctg aaccagcagg ttggtaaccc tattgcccgg acaccccatc
     1261 tggcgcgaaa tggtaaccac cggccctggt tctttctggg tattgttggg ctgacgattc
     1321 ttttcaacca gatatttgag tacggtatag ctcatgggtt aatgtataaa atgatattta
     1381 acattggcta aatataactt ttttttccta ttaagtcaat aaattttcta aagaaactgt
     1441 catgtttttg taaacataac cacccacaag caaccgagcg ttttttgctg ttccggctaa
     1501 gccaaaacat gtgtgttcca cttgctcaac ccttttacct ataagttact ggctagggtt
     1561 cacacaacca gatatataaa tattttatgc ggttgaattg ctgtttaaag cctaattgat
     1621 ttctaatagc attttaccca tttttacttc ccctgtaaac ttcagctgat agttacacca
     1681 caaccggaat tttggtttca ggagccgcca tcatggcact gctgttggta gggttgagtg
     1741 aggattttgg ccaacatgga aacgcaatac aaccgcttgc ctttgaagat aatttgagaa
     1801 gaatttaatg cgaaatgaat gtgcgggtag ccaaaatccc ttttggcttg tgtggggtga
     1861 gccagcaagg ttatgaagca gcaggttgga tgtgtcccgc tgttcagaat cgcatccccc
     1921 actgtcattt tttagttgcc gcttgagccc taaaaaataa ggggaatacc ttgctgattg
     1981 tcataataac acatttaaaa tggaataatt aacaggaagc tgcctagatg ctctcctttc
     2041 ctgaaataac atggaggagc ttcaccacaa aaatgtacgt gataggaatc agggaaaggg
     2101 agttggccag tataagcggg aagtctctaa gcattattcc gtaaaccagc caaagcgcaa
     2161 ttgccacctc aactatgcag tacatataca gggagatgct ttgcgtgtcc ttggttttca
     2221 tcaccttgta ggcttgggga aactggttta gagttgagag gattgcggcc agcagtccga
     2281 taatttgggc aaccataatt atttatgttg aggttaagcg gtagcgtcga gatttttctg
     2341 gtgtattttg gagtaaattt ttgggtcaaa tctgaatagt gacggaggtt tatggcccac
     2401 gccgtactgt ttctcgttgc aggaaaccac gtacttgagg ttggcaattt tgcggcggaa
     2461 attccgctta tcgaaacgtg tacccagaat cacttcgtaa atctgttgaa gctgggtaag
     2521 ggtgaatttc ttgggaagca gctcaaatac tatggggtgc atggttacat cctccttgag
     2581 tttttttagg gcctcaccga gaatttcctt atggtcgaaa atcaagctct ccacctcgtc
     2641 aacattatgc cattcggcaa catttagaag tttagaagaa tcaaagttta gcaggtttac
     2701 cagcgagtaa aaccctacgg taattaccct atcctcccca agtccgtact ttttaatcca
     2761 ttggtagtcc atttcgcgcc ggcgtaagcg gtccaaatcg ctaaatacca tgaactgctt
     2821 cataaagatg ttctccacgc cggcttgctc cttgagtacc ctcgtggctg tatcttctat
     2881 ccgctcgttc atccccacgt ggttccctgg aagcttccac tcgatgtgaa caatatcctt
     2941 gtactcaatc agctcctttc ggagcaacac cttcagcaca ccctcatccc acccgaaaat
     3001 tacacaatca atcgaaaggt tgggtattag tcttaactca tcatccatac caacgtatat
     3061 ttggctgcaa gcttagctta aaaattacga aagccccacc agtttatctg ccaaatttaa
     3121 ttcaaaaaat agccgaagtt gttttttttg aaaaataagt gttaaaaata cacttttatt
     3181 tgattctaat aattacctgc ttatatttgt attttaaaat tgtcaaaaag acacttataa
     3241 agcacaaaag aattttgtta cctacactta agaatatgta tagaataaga ttgtggccat
     3301 tgggaattgc agtaattgca ttgatttttg gctcttgcaa cgttgaaaaa agaagcgatg
     3361 ctgaacaggt ggctaaggcc atgctggata ggatgactct tgaggaaaag gtgggacaga
     3421 tggcccagat tactcttgat gtggtaggaa agggtgatag cctatttaat agctatgagc
     3481 cttttgagct ggatacggct gaactacggg aagcgattgt tgactaccat gtgggttcag
     3541 tgctaaacag cagcaataat cgagctcgct cactggctgc gtggaacagc atagtttcta
     3601 ccattcagaa tgtagccata gctaaaactc ggctagctat accagtttta tatggaattg
     3661 atgcggttca tggcgctaac tatacggctg aggccactct ttttccacag cagataggca
     3721 tggcggcaac atggaacgat tcccttgttc aggcaattgg aagcattaca gcctacgaaa
     3781 cacgtgcaag tggaattcca tgggttttct ccccggttct tgatttagga gccgaccctc
     3841 gtttcccccg acagtatgag ggatttggcg aggatccgca cttgtgcagt gttttggggg
     3901 ttgcgctagt aaatggctac cagggagcaa gtgtatcaag tccgctgaaa gttgctgcct
     3961 gtgccaagca ctacctgggc tactcggcgc cctcatccgg caaggataga actccggctc
     4021 agctgccaaa cattacgcta aaggaatacc acctgcctcc atttaaagct gctgttgaag
     4081 cgggggttaa aacgtttatg gttaactccg gggtaattaa cggacttccg gttcatgcca
     4141 gccactacat tattaccgag ctactgaagg aggaacttgg ttttgaaggg gtagtggttt
     4201 ctgattggaa cgacattgaa aacctttacc gtcgcgatag ggtcgtggcc actgaaaaag
     4261 aggctatagg gctggctgta aatgccggaa ttgatatggc tatgattcct taccgttacc
     4321 aggatttttg taaccagctg gtggagctgg tgagggaggg gtcggtagag gaaagccgaa
     4381 ttgacgatgc tgtgatgcga atacttaagc ttaaggctga acttggattg tttgagcgtc
     4441 caactacagt gctgggcgat taccccaagg ctggctccga ggagtttgcc aaagtttcat
     4501 accaggcggc aggcgagtct attaccctgc tcaaaaatag caacggcatc ctgcccttaa
     4561 gcaaggataa aaggtataaa attcttgttg ccggccccaa cgcaaactcc atgagaaccc
     4621 tcgatggtgg atggtcatac tcgtggcagg gtgagaaggt agaagaattt gccggcaggt
     4681 acaacaccat ttttgaggcc attaaaagta aagtgcccca atcggaggtc agctatattc
     4741 ccggggtaag ctacccggct tacggacgat actttgagga acagtacgat aagttcgaag
     4801 agtctgctgc tgccgctcgt aaggccgatg ttataattct ttgccttggc gaaaacagct
     4861 atgctgaaaa acccggcgac cttaacgacc tgtacttggg gagcaagcag cttaatttgg
     4921 cagaagagtt ggccaaaacc ggtaagccca tcatacttgt tttaaacgaa ggacgaccca
     4981 gggttattag ccctattgaa cccatgatgg cagcggtagt tcaaacctac cttccgggta
     5041 gctatggtgc cgacgcactg gccgacatcc tttttggcga cattaaccct tccggcaggt
     5101 taccctacac ttaccccagc tacccaaact ctaccgttgt ttactaccat aaaccagccg
     5161 aggagcaaaa gccaaatatt ggagcctaca gctatgaatc ggactataac ccgcagtacc
     5221 catttggcta tggacttagc tacactaatt ttgaatacag ccagctgacg gttaaccgaa
     5281 gtgagcttaa gcaaaatgat accctaacca tttccgtaaa tgttcggaat actggagata
     5341 gaaagggaaa ggacgtggtt cagctgtaca cttccgactt agttgccacc attacccccg
     5401 atgtgaaacg ccttaggaag tttgccaaaa ttgaacttgc cccaggtgaa gctaaaactt
     5461 taacttttaa gctcgttacc agtgagcttg cttttgttga ctccaatgat aggcttgtag
     5521 ttgaacctgg agagtttata gtttcggttg gaaatctaag caccaaattc catatagtaa
     5581 actaatgttt caacccttaa atctaaagct atgaagtatt tcctgaaaat ttttagaaga
     5641 aatgcacgca tactcctact gggattgcta tgcgtgtgtt ggattccttc aatggcgcaa
     5701 actattacgg tttccggaag ggttaccgat aaggagggag cgccaatggc aggtgtaaat
     5761 attgttgtaa agggaaccac gaatgggaca aataccgacg tggacggaaa gtatagcata
     5821 agcacaccgg cgaatgctat tctacagttc tcctttattg gtatgaagag cgttgaggag
     5881 gctgttggcg gtcgttccat tattgatgta gttctcgaaa gcgaaagcat ggacatttct
     5941 gaggtggtgg ttataggata tggcaccagc cggaaaaagg acattaccac cgctgtttcg
     6001 gtggtttcga ctgaagattt tgacaagcga cccgtaacca acgtggcaca ggcaatacag
     6061 ggaaaggccg caggagttca ggttgtgctt ccttcgggta aacccggagg ggatgcattt
     6121 atcagggtta gaggtgcaac atcggtgctg gccggtaacg accccctgtt tgtaattgat
     6181 ggagttccta ctaccagcat gcaagcactt aacccgcagg atattgaaac ggttcaggtg
     6241 ctgaaggatg cctcttctgc cgcaatttat ggcgcacgtg ctgcaaatgg tgtggtaatt
     6301 attaccacca aacatgcaaa ggccggtgtt gcttcagttt cggttaatag ctacatgggc
     6361 ttctcaaacc tggctaactc actggatgtg ctcaacaccg agcagtaccg ggagttaatg
     6421 gacgaagtaa agggaccagg aactgttccg acctcccaga ctaactacac caattggatt
     6481 gatgaaacct tcagcaccgg acaaattcaa aactaccagg tttcggtttc caacggtacc
     6541 gagaagatga agtactactt ttcggctggt ttttccgatg aaaagggtat tgttaagccc
     6601 gctgaaaata aaaagtacac cattagaacc aacatcaaca gcgacctgtt caagtggctt
     6661 aaatttggaa ccaacatcag ctttattcat aactggtaca acgatgtaag cgataatgca
     6721 aactccggaa gggcaggtac catccttagc gccatcaaca ccccaccctt ccttcatatc
     6781 tgggatccga ataaccccga acagtacgag gttaacccat tccagaattc atgggaaaac
     6841 cctctggctt atgcttcgcg tatcaaccag tcgaaaactg agcgggtgct ggccaacgca
     6901 aataccgaga ttaccttctt aaaggatttt aagctgaaat caaatttcgg gattgatggg
     6961 tatatttacc agcgcgatct ctacatcgac cctgttaaaa ccgtttatgg acgtacggaa
     7021 aacggaattg gcgaggccga taagtcaaac tccttggttt ggttgataga gaacgtgcta
     7081 tcctacgaaa agacctttgg caaccacaac ctctctgttc aggcaggtca gtcggcccaa
     7141 tcgtcacgtt gggaccaatc ctacatatcc gctaaagact ttccaaagaa ttctgctgta
     7201 actaccatta atgctgctaa ccagattacc tatgctgcat caagcgcttc ggaatgggca
     7261 attgcatcat tttttggacg tgtatcctat aactacatga gtaaatacct ctttaccgct
     7321 aacttccgct acgacggttc ttccaagcta tcctccgata accggtggga cttcttccct
     7381 tcattctcgg ctgcatggag gttatcgtcc gagccatttt taaagaatgt cgtctttatt
     7441 aacgatttga aaattcgtgg tggttggggt aggaatggaa accaggaggg aattggcgat
     7501 tacgcatggc aggctcttta tggtttttcc cgtcaatccc ccaccagccc gctttcggga
     7561 cccggtattt accagatgaa cctggagaac ccttcgctta aatgggaggt tacttaccag
     7621 tccaacgttg ggctcgactt aaccgtgctg gactcgcatt tgagcttcac cggcgatgtg
     7681 tactggaaga ataccgatgg cctgctggtg aatgttccac tcccttcggg ctctgctgca
     7741 gttgattaca ttacccgtaa cgatgggaaa atgaaaaact ggggcgtgga gctgtcgctg
     7801 gatgccaagg acttcaggca tgagctgaaa tggagcacaa atcttttttg gtcgatgaac
     7861 cgcaacgaaa ttaccgaact tcagctttcg cagatctaca actttgccca gctcgaaagc
     7921 aacaacgagt acgttatccg gatgagcccg ggttactccc ttggaacatt ttacggatat
     7981 gtggcaaagg gagttgaccc cgaaaccggc gatatgattt atgaggacag gaataacaat
     8041 ggcgttattg accctgacga tcgaacggtt attggttcag ctgaacccga ctttatctac
     8101 ggcttaaatc aaaccttaac ctacaaggga ataggcttaa ccctgttttt ccaaggttca
     8161 caggggaatg atattttcaa cgcctcccgg attgacaccg agggcatgtt tgacgtgaaa
     8221 aatcaaacaa ccgaggtgct gcgcaggtgg aagcggccgg gtcaaatcac cgatataccc
     8281 cgcgctgtga gcaacggtag cactttcaat gtgcacaact ctacccgctt tgttgaggat
     8341 ggatcatact ttaggcttaa ggctcttacc ctgtcctact ccttaccgtc aaaatggctc
     8401 agcagggtta gcattaaaaa cttcaccctt tatgcaactg ctaccaacct gtttaccatt
     8461 acaggttaca acggattcga tcccgaggtt aactacggtg gaaacaacag cgtaattcag
     8521 ggtgttgact acggcaccta tccgcaatcg agatcataca tatttggttt atcgttcgac
     8581 ttttaaactc cgagagatat gaaaaaactt aatggatacc tgcttgccgg cgttgctctt
     8641 ttaacattca cggtggtttc gtgtgaggac tttctcgaca aggaccccat ttcgaactac
     8701 tccaacagca cgctgggcgt tgataataat actgacactc taaagtacac tactgccgaa
     8761 caggcagaag cgctgctggc cacctgctac tccaacttca ggggcgagct gtttcaactc
     8821 gactactttg tcaacggcga tatgcagtcc gacgatgcct acgccggtgc cagcggaacc
     8881 gagttttccc agtttgacaa cttcagcatc gacgctacca acaagaacgt ggagcgcgat
     8941 tggaagtacc tttacaagat ggtaaccgat tgcaatacgg taattgtaaa cgttcctaaa
     9001 attaccgacc aggcattaac cgatgcccgt aaaaaggaga ttgttgccga ggcatcgttt
     9061 attcgtgcat gggcatattt cgaccttgtt cgcctttacg gcgatgttcc cctggtgctc
     9121 gatgtggtta ccaccattac agccgataat gttgccgagg tttaccccct cctttaccct
     9181 aaaagggact ccagggagtt ggtttacgat cagattatta ccgatttgaa tacagcgctt
     9241 gatggtgtgc cggttactgc tcccaacaag ggctatgtaa ccaaaggtgc tgttaatgcc
     9301 ttgctggcaa gggtttatgc tacccgccag ccaattgact acacaaaggt gaacgactac
     9361 tgcgatgccg tgattaacgg tggctacagc ctgttaagca actacgacta cctgtgggat
     9421 tttcagcacg aaaactccag tgaagcaatt tttgaaatcc agtacgacgg taactggaac
     9481 accggtacag gcaactgggc tgttagcatg ttccttggca cgggatggaa gaaattcaac
     9541 accccatcca acgacctggt ggccgctttt gatgcagaaa atgacgttat ccgcaaaaac
     9601 tcttcaatta agtttgagga tgttacaggt agctggactg acgccaactg gcccacatcc
     9661 acttacccat ttatttataa aattagagac gaggcaagta cctccaacat aattctcatg
     9721 cggctggccg attttatcct gctaaaagct gaagccctta atgaacttgg tctccctggc
     9781 gccgatacgc tggttaacca gattaggggc agggttaacc ttccttcggt ttcgcctacc
     9841 acaaaggagc aaatgaggct gattattgag aaggaacgtc gcttggagtt ggcttttgag
     9901 gggattcgct ggttcgattt acttcgaaca ggacgggcca ttacggtgat ggaaaatgtt
     9961 cgtgggggca atggcgagtt gctgtatgcc gggaaactcc aaccatataa actcctttgg
    10021 cctattcctc aagaacagcg cgatttgaat agcatgttaa cgcaaaatcc tggttactaa
    10081 aatcatataa atttgatacc atgaagatct taaaatatac gtgcattgta ggtttagcgc
    10141 tggttgcatt tacagcctgc gaggacaatg aaacaaaaac tacctctcaa cctacaataa
    10201 gtaatgtttc catctcccca accgccgggt taacctatgg tgattccatt gccgtttctg
    10261 cttcggtttc cgatgaggta acgccactat caacccttga cattcaggtg attgtggctg
    10321 atgtggaaat ctataaaaag tcaattagaa ctaagggtaa cagtgctaat gtttccgcta
    10381 agttcaaact gccctttgct gccaatgccg aggataatgg tgaggttacc gtgaagctcc
    10441 agtctatcaa cattgatggc ttttctactt ctcaaaccga aacgctccat gcagcccgac
    10501 ctatacttgg cgatatcctt tacctggtaa cttcgagcga taccattgag ctggcaaaaa
    10561 gccctgaaaa cccgttcctc taccttagcc ctacggggtc gtaccccaac gaggttgttg
    10621 gtaaaattgc aacctccgaa aaccttgaag ctgccgagtg ggtttgggtt tcctcgggta
    10681 gtgcaattgt aaccggcagc tacaccgata gcgatatcag gcttagcgat ccatcctacc
    10741 aggttgagca gctttccttc aacgtcatga cttttgagct gaagtttatt ggcgagctgc
    10801 tgcccagcgt taagtttaac accaccacgc tgcttatggg tgaggatgga cgtttccatg
    10861 gcgaggtaga atttaccaag ggtgatgtgg ttaccgtaga gggaatttct aacctggcag
    10921 ctgcctacaa ccctgacttt ttcgactaca aggacggcgt tcttactttt aaagccccca
    10981 ccggaaagta ccaggttgtt tactgggctg gaaccatcaa ctacatgtgg ctgcggcagg
    11041 ataatgccgt ttaccccgat gccctttggc ttaccggtgc cggaattggt atgcccacct
    11101 ttattaacga tgtaacgaat atctggtggg gatgggatga ttggggcgta accacacgcc
    11161 aaagctactt cttttgtcca aaaattggcg agaataagta ccaagttact gtttacttga
    11221 tgaataccga ggggtcgtgg tggttgcaaa ccgagtttaa gctctttcct actaagagtt
    11281 gggataatga gttctcctca atggattggg ggtacatctc cagcaactta agtcccaata
    11341 ccaatgggaa ttttgttcac acccccgagc taaccgaagg gctgtacaga ataaccattg
    11401 acatgagcgg cggaaagggg aactatttcc tgattgccca gaagattaac tagggttggt
    11461 tttaggtttg cgtgcttatg ttgaagaatt aacctgtttg gattggcata gtcctacagg
    11521 ttaatttttt tccctccgtt tgtgttaaaa tcctttttaa aatagattgg gttcagctaa
    11581 cttttgcact tcaactttac aatgtccatc tcaattcacc cgatgaaaaa tatacataac
    11641 agcctatttt cttttgcttc cggaatgctg tcggcatgtt tgattttttt tgccatcctt
    11701 ctgcctggct gcacggatga ttctgacagt gtagataatc cgcccgacga caccacccaa
    11761 ttaggtgatg ttcgggtttg ggtaaccacc tgtaaccagg cttacctctt caaagagcag
    11821 tcggtttcct tttcagacga cgtggcgccc ttcaccatac gggttgatac ccttaaccga
    11881 taccagcagg ttgaaggttt tggtgctgca cttaccggtt cgtcagccta taaccttagg
    11941 cgtatgacag acagccaacg gacaagcctg cttacagagc ttttcgatcc ggtaaagggc
    12001 attggactta gctacctgcg ccttagcatt ggctcttccg atttttccct cagcctttac
    12061 acctactgcg actacccatc tattgattcg tttgccctgc atcctactga ccttcgcgat
    12121 ttgattcctg ttcttaagga aattcttgcc attaatcctg ctattaagat aatggctaca
    12181 ccatggacac ctcctatttg gatgaaaaca agtgctgcat ggagtggtgg cagtcttatt
    12241 cccgacttct acgacgagta cgccgagtat tttgtccgct acattagggc tatggctggg
    12301 gagggtatta ctattgatgc cattagcgtc cagaacgagc ccatgtggga gtttggtact
    12361 cccggcatga agatgacctg gcaggagcag ctcaccttta ttagagattt cttaggacct
    12421 aagtttgctg cagaggatat ttcgaccaaa attcttattt gggaccataa ctgggatatg
    12481 cccgactacc ccataagcat tctttccgat cctgctgcct acatgtacat tgccggttca
    12541 gcattccacg ggtatggggg cgagtactcc gccatgctaa gcgttcacaa ccagtttccc
    12601 caaaaggggc tatactttac cgaaatctcg ggcgggggat gggcaaccga ctttgacgat
    12661 aacctgaaat ggaccattga gaatgtattc tttggaaccc tttcgtgttt ttcgaagaat
    12721 gtgcttctat ggaatctggt gctcgacgac aattggggac ctttcattca gggcgggtgc
    12781 tccaactgcc gtggggttat cacccttaac tccgatggaa gtcttacccg gttcaacgag
    12841 tactacgctt tggcccattt tgcaaaggca ataagaccaa atgcctacat gctgggtaca
    12901 acagttgtag gtgatatgtc ccagctggca gacttcaagt atgttgctgc tcaaaatccc
    12961 gatggttcaa aggttgtagt tgccatgaat ttttccagcg tcgggaaaaa acttgctgta
    13021 aggtgtggta gcaataagtt caccttcacc gtccagcccg aatcgctggt tacattcatg
    13081 tggaggtaag gtaaaagata aaggataaag aacttgtccg cctactggcg gaacttgtc
//
LOCUS       DAOSHM010000063        12466 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_4250_length_12466_cov_11.305777, whole genome shotgun
            sequence.
ACCESSION   DAOSHM010000063 DAOSHM010000000
VERSION     DAOSHM010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 12466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12466
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4250_length_12466_cov_11.305777"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..968
                     /locus_tag="PK839_08810"
     CDS             <1..968
                     /locus_tag="PK839_08810"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HOG72874.1"
                     /translation="AGIPETSPAMTINKLCGSGLRAVSLAAQFIMLGDAEVILAGGTE
                     SMTNAPYLLSKARNGYRMGHGQIIDSMISDGLTDAFNQYHMGITAENIAEQWGISRRE
                     QDEFALNSQHKAERAQASGRFDDEIVPIEIPQRKGDPLIFKTDEFVRKGLTAEQLELL
                     KPAFKKDGTVTAGNASGINDGAAMLVVMSEKRAEELGITPLVKIVSYGNAALDPKIMG
                     YGPVPATRAALKKAKMEVSDIDLIEANEAFAAQSIAVIRDLKLNPEKVNVNGGAIALG
                     HPIGASGARILTTLIYEMKKRNAATGLATLCIGGGQGTALIIENM"
     gene            1139..1882
                     /gene="fabG"
                     /locus_tag="PK839_08815"
     CDS             1139..1882
                     /gene="fabG"
                     /locus_tag="PK839_08815"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HOG72875.1"
                     /translation="MKRLENKIAVITGGADGIGKATSLMFASEGATVVIWDLNEQKGN
                     EVVSQILAEGGKAEFLNVNTAQFQQVENATNIVIGKYTRIDILINNAGITRDATLKKM
                     TPQQWQEVIDVNLTGVFNCTKCIGQYMVEKGYGRIVNASSVVALYGNFGQANYAATKA
                     GLIGITKTLAKELGRKGITVNAVAPGFIATEMVKKMPAEVLKSMEEKVPVGRLGYPEE
                     IASAYLFLVSEEAAYINGAVLSVDGGITI"
     gene            1969..3537
                     /locus_tag="PK839_08820"
     CDS             1969..3537
                     /locus_tag="PK839_08820"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72876.1"
                     /translation="MATTAQVIGTWILIAIYASAILFFVFKGASRIKGITDYALGSFS
                     FSPVYVGLSLAAAMTSAATFVINPGLIANYGVSGVLSYAVFFPLATVTSLIVLTKSFR
                     RYGQSVQAVSIASWIGNRYKSQGFSIFIAFLSIILIAFIVLILVALTKVFASALNANE
                     ITTLAVVVIFVFGYMMFGGANSMVYTNTIQAGIMIIVALILILSGIPFFKDGFSGFFQ
                     SLASIDPMLVKPTNPTSPLFRDFYEIIFAQIVVGAAVVCQPHIITKSLMLKKESDVNK
                     FLVTSVVVEILFFAVVIAGLYARLVFPDLTLSGQALGNDSIIPSYVVKVFSNGVVSTL
                     IGLLIIMGLVSAGMSTLEGLIQSISTSITNDLIKPLWSSKKNISDKQYLSINKIAIVV
                     LAIASFIIARNQLLQPKLSVAILAQNGSYAYFSTLFVPILMGIFVKNVRKWIPFIAAI
                     VATAVHFGVFYFLPYLHTKFTVDFGWFNKYIEGDVRNPAIAASTAIVLSIAVGFGLHF
                     IWKLKPAKIDVANS"
     gene            3550..4860
                     /locus_tag="PK839_08825"
     CDS             3550..4860
                     /locus_tag="PK839_08825"
                     /inference="COORDINATES: protein motif:HMM:NF014594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HOG72877.1"
                     /translation="MDIESVYKSKLINAEQVADLIESDTDIIVAQCASEPQGCMGKFH
                     LAKDRVHDVKVFSVLTLKPYDFYMKPEMKGHFELCSWFHAPGSRQALKEGTGTVTYVP
                     NMLHRAATDRLKVRRPHMFVGTCTPPDEKGYVSLSLGVTYEKDIFDVAELKILEVNKH
                     LPRTFGDTHVHISEVNYFVEHHQIPPTLPSPQPDEEAMKIGEQVAKLVEDGATIQLGI
                     GEIPNAVALSLKGKKDLGVHTEMMVDSVMELYEQGVITNKKKALHKGKFICTFAMGSE
                     KLYRWLDNNPSVEFLKGSYVNDPCVIRKNSKMVSLNTCIMIDFTGQVASESIGIEQYS
                     GTGGQTDTAEGAVEGFDGLGKSIIACRSTAKRGQISTIVPVLPEGTGVTLHRSHTDYV
                     VTEWGHARLTGLTVRERTKVLISIAHPKFRDELTAKAKQMGYLY"
     gene            4880..5893
                     /locus_tag="PK839_08830"
     CDS             4880..5893
                     /locus_tag="PK839_08830"
                     /inference="COORDINATES: protein motif:HMM:NF020130.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HOG72878.1"
                     /translation="MIPVRIKSTGVYAPGLPIGNQELKNLAKIDFDTAKTESKIGIVQ
                     RHIAHLRGINETAADFATKAGVEAIANAGLAASDIGLFIVATDTPEYISPSTAIVVQG
                     RIQKGEVWGGAFDINAACASFSIAFDTASRILANNSLIRFALVIGVYNMPAFFRPGDA
                     FGYSIFADGAGAFVLERVEGTESSSGYIGGQQLTDGTQWNYIGIYSGAARKPITHQML
                     DSGEYGLQNLQPLPGDRNVRLWPMVVEKLLNGHSVKLEEVDHFVFTQINHSVIVKVME
                     VLKQPMTKTTCVMDRYGYTGSGCLPMAFHHAVSEGRVKRGNKVVFVASGAGLAVGSNL
                     FIY"
     gene            5906..6922
                     /locus_tag="PK839_08835"
     CDS             5906..6922
                     /locus_tag="PK839_08835"
                     /inference="COORDINATES: protein motif:HMM:NF005308.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase"
                     /protein_id="HOG72879.1"
                     /translation="MNNHVGIVGSGVYIPERRMTAREISEATSGVWSEKAVVEKLGIR
                     QIVIPGPNDGTQEMGVKAALDALKNTSTDPKSLDLILCMGEEWKEYPLTTSGIYIQEQ
                     IGATNAWAIDVQQRCGTTVASMKIAKDIMLSDPDISTVLIVGGYRNGDFVNFKDSSVS
                     FMYNLSAGAGALILKRNYGRNLLLGTHIITDGSLARDVGVEFGGTANPITHDNVHEAY
                     KSLRVFNEQHMKSRLNDVSMSNWMKCIDMAFKKSGVDRDKLGYLAGLHFKRSMYESMV
                     HELGLTMEQTIYLEEFGHMGQVDQILSLHLGLQLGKIKNGTIISMIAAGIGYAWGANV
                     IRWG"
     gene            7110..7616
                     /locus_tag="PK839_08840"
     CDS             7110..7616
                     /locus_tag="PK839_08840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72880.1"
                     /translation="MRKYLAIALLFSLALVACDKSDEEEKYDPAKVGIVGEWYSAGSD
                     VAVLLSTYFAVDSIYAKFNDNNTYTVESYSQGAKTTMTGTYVQAKSNVGSIWTITLNQ
                     TTPTALTSEGIFEITLTSGKYVMRYEVVQTSPNIGATPPTPAAGFGSSNGGALQTMNI
                     QTYRMLVR"
     gene            7691..9289
                     /locus_tag="PK839_08845"
     CDS             7691..9289
                     /locus_tag="PK839_08845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72881.1"
                     /translation="MKEARHLITACFIIAIYSASAQNPSNQYFRGEIPEKANKEFQFM
                     AFFINQGVTSNIYAQNDFLKGQVVGRLFGGNTTRTSDSLTSAYLEQRIIPFFIYQPKL
                     FNGKATLRASFEIDWTWGDVSYGTGGNFGSAVSGDQVNLQTQNIELELNPAKGWAINL
                     GLQRMFDSPYNPYKAAVDKMGVTGYRLAYWGTDAVGVSVRKDWDYSRAKVGMYKLYEN
                     YIQDDDDVTMYELMGERDVSRLWKVGASVNYVRDRASGAGGVSILGQGLNSLLASHNG
                     VYRFNFGNVPYKADIFWMGAFFGRNTEHWLDPWMVTGFVNFNIGTADTLLNQNVSLSK
                     WKKSTDIFGFAGNLRFSYRYGQTLNDNVTLDVIYSSGDGDGLTDGQYSGVITGNQWGA
                     PAAIFITSGSYILMPHGNVVNRFSPAIMDISNMGYGLTAATLNFSRGIIPHRLNAKVG
                     SAIAFSNIQPAGGGYYMGTEINGNVAYNFGPFMSLELHGAYLMLGDFYDSKQAAYGSP
                     VNGGLDKRPVNPWTVFMVFKWLLF"
     gene            9418..10368
                     /locus_tag="PK839_08850"
     CDS             9418..10368
                     /locus_tag="PK839_08850"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOG72882.1"
                     /translation="MKHVVTYCLVAVMVFTGCSTPYKSLKSISSMDEITYKFPVKKVM
                     LPKSGYEIAYNDEGSGSKTIIFIHGVGSYLRAFEKNVEVLRENYRCIAIDLPGYGKSS
                     KQPHSGMMSFYAGIINELVEQLNLGKVVIAGHSMGGQIAMTTALLYPDIVESLVLVDP
                     AGFERFHKGQKQWFRDVMTFEGVKYTTPEGIQNNLATNFYRVPKDAEFMITDRIQMRT
                     ASDFDAYCYAVVQSTIGMVDEPVIDYLKDISVPTLILFAETDNLIPNRYLNPGRTIDI
                     AKSGASQIPNSKLVMVPKCGHFMMFEKPDVFNANVLEFLK"
     gene            10382..10780
                     /locus_tag="PK839_08855"
     CDS             10382..10780
                     /locus_tag="PK839_08855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72883.1"
                     /translation="MEAVVIGEHCKVSYIEEVPCIMIQWFGLPPTEEFRMGCNTVLEM
                     MKKYSATKVLTDNSHVKVFSVTDQKWLNETWLPKAEALGYRVSATLIAEGDPFVTFAV
                     NNIMANRNPNKFKARYFTKYDEAIQWLKEC"
     gene            10863..12296
                     /locus_tag="PK839_08860"
     CDS             10863..12296
                     /locus_tag="PK839_08860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72884.1"
                     /translation="MKDKLATFQSLCDELYPHELDYLLSINQFQKGENVQVLQLVYYN
                     ATNPDNPIPFDKSLDKRTYSYIKSWILDNLQKIDVDVFYDWVSGVERIILNDSIQSAD
                     EKQLLKMMEVIKPTDYYFMKFFRVIQHYRDYLLVRNRPRNYELVTDFLARSKSSYLYS
                     QSLNNKMNEAAEKVIYRKIWVPEEFNVWEKLFSDIYYNETIDGYTRYRAVVRLTILYY
                     TQREFDKLKLVYQHLDQQFKTPVFYSKRILANYYANRAMMHSKLNELEEAEKFGMLSI
                     RHRNSDYLFYLVNVCDVLLRANKAPKALKLLVNSFPDLKTSGNFYYKIGFASLYIKTL
                     VANNMTKNAVDYGEIFLNGYKDEIFEYRWHLFFSSYIGALFRFEKYSKILALGKRYKL
                     CAQERQLLGTAQYLPLIQLYMLASEYMEGVTTKDKLRQAIMQHIQEVGQNRFRHRKVL
                     DTLKTLSLCIPDVISGIMVTNQIHEEI"
BASE COUNT         3649 a         2659 c         2820 g         3338 t
ORIGIN      
        1 aagcaggtat tcctgaaaca tcaccggcaa tgaccataaa caagctatgc ggatcgggtc
       61 tgcgggcagt ttctcttgcc gcccagttta tcatgctagg tgatgcagag gtaatactag
      121 ccgggggaac cgaaagtatg accaatgcgc cctaccttct gagcaaggcg cgcaacggtt
      181 atcgcatggg acatggtcag ataatagata gcatgattag cgacgggctt accgatgctt
      241 ttaaccagta ccacatgggc ataaccgccg agaatattgc ggagcaatgg ggaatcagcc
      301 gtcgagaaca ggacgaattt gctttgaaca gccagcacaa agctgagagg gctcaggcat
      361 caggaaggtt cgacgatgaa attgtgccta tcgaaattcc ccagcgaaaa ggagatcctt
      421 taatcttcaa aacagacgaa tttgtccgta aaggattaac cgccgagcag ctggaactgt
      481 taaaaccggc ctttaagaag gatggtacag ttacagctgg aaatgcttcg gggataaacg
      541 atggggcagc catgctggtt gtcatgtcgg agaaacgcgc agaagagttg ggcataacac
      601 cgctggtaaa aattgtgtcc tatggaaatg cagcgctaga ccctaagatc atgggctacg
      661 gacctgttcc tgcaacacgt gctgcgctga aaaaggctaa aatggaggtg tccgatattg
      721 accttattga agctaacgag gcgtttgcag cccaatcaat cgcggtaata cgcgacctta
      781 agcttaaccc tgaaaaggta aacgtaaacg gtggtgcaat tgctcttggt caccctatag
      841 gtgcttcggg agcacgaatt ctaaccacgc tgatatatga gatgaaaaag cgcaacgcag
      901 caactggtct ggcaaccctg tgtattggag gtggacaggg aacagcacta attattgaga
      961 atatgtaagc taattaagct aaatcttttg agacatccat gattatacta agcataaaca
     1021 caaataaaca aaacatgggg tttaccttca gcaagtttta tggactttat tcgttattga
     1081 gtgtttgaaa accaaaactt tattcgatgt caactaataa tgttaaaatt tattactaat
     1141 gaaacgattg gaaaacaaaa tcgccgttat tacgggtgga gccgatggta ttggcaaggc
     1201 tacatcgctg atgtttgcca gcgagggggc aactgttgtg atctgggatc tgaatgagca
     1261 aaaagggaac gaagtggtat cccaaatact ggccgaagga ggcaaggctg aatttttaaa
     1321 tgtaaatact gcccaatttc aacaggtcga aaacgctacc aacatcgtta ttgggaagta
     1381 cacaaggatt gatatcctta ttaataatgc cggaataacc cgtgatgcca ccctaaagaa
     1441 aatgactcca cagcaatggc aggaggtaat tgacgtaaac ctgaccggtg tttttaactg
     1501 cacaaaatgt ataggccagt atatggttga aaagggttat ggccgtatag tcaacgcctc
     1561 ttcagtggtt gcgctatatg gcaattttgg gcaagccaac tatgcagcaa ctaaggccgg
     1621 ccttatagga ataacaaaaa ctttagcaaa agagctaggc agaaaaggta tcactgttaa
     1681 tgcggttgca cccggattta ttgctaccga aatggttaaa aaaatgccag cagaagtgct
     1741 taaatcaatg gaagagaagg ttccggttgg taggttggga tatcccgagg aaatagcttc
     1801 tgcatacctt tttcttgtaa gcgaggaggc agcctatatc aatggtgccg tactgagtgt
     1861 agatggtgga ataacaattt aaacctgttt ttcacaatac cttaaaacca caaaggaatg
     1921 aaatctaccg ctaacccaat tttaaccaaa caataaaaaa ttaaagctat ggcaaccaca
     1981 gcacaggtaa tcggaacatg gatattgatt gcgatttacg cttcagcaat actattcttt
     2041 gttttcaaag gcgcctcccg cattaaggga ataaccgatt atgcattggg aagcttcagt
     2101 ttttctcctg tttacgtagg actgtcactt gctgccgcaa tgaccagcgc ggcaacattt
     2161 gtaataaatc ctggacttat tgccaactac ggagttagcg gtgtgctatc ctacgctgta
     2221 tttttcccat tggcaaccgt aacatcgtta attgttctaa ccaaaagttt ccgacgttat
     2281 ggccaatccg tgcaggctgt atccatcgca agttggatcg gtaaccgcta taagagccaa
     2341 ggattttcaa tattcatagc tttcctctca attattctga tagcttttat tgttctaatc
     2401 ctggtagcgc ttacaaaggt ttttgcaagt gcattaaatg ccaacgaaat tacaacatta
     2461 gctgtagtgg taatctttgt ttttgggtac atgatgtttg gcggggcaaa ttcaatggtt
     2521 tataccaaca ccatccaggc aggcatcatg attattgtag ccttaatact gatcctctcc
     2581 ggaattccct tctttaagga tggattttca ggttttttcc agagccttgc cagtattgat
     2641 cccatgttgg ttaaacctac taaccccact agccctctct ttcgtgactt ttatgagata
     2701 atttttgctc agattgtagt aggcgctgcg gttgtttgcc agccccacat catcaccaaa
     2761 tcactaatgc tgaaaaagga gtcagacgtt aacaagtttc tggtaacttc ggttgttgta
     2821 gagattcttt tttttgccgt tgttattgcc ggactatacg caagactggt atttccggac
     2881 cttacgctaa gcggacaagc acttggtaac gatagtataa ttcccagcta cgtagtaaaa
     2941 gttttttcaa atggcgttgt ctcaacactc atcggcttgc tcatcatcat ggggctggtt
     3001 tcggccggga tgtcaaccct ggaggggctc attcaatcaa taagcaccag cattaccaac
     3061 gacctgatca aacccctttg gagtagtaaa aaaaatatat cggataaaca gtacctttcc
     3121 ataaataaaa tagctatcgt ggttctagct atagcatcct ttattattgc tcgcaaccaa
     3181 cttcttcagc ctaagctaag cgttgccatt cttgcccaaa acggatcgta tgcttacttt
     3241 tcaaccctat ttgttcctat tttaatgggg atatttgtta aaaatgtcag aaagtggatt
     3301 ccgtttatag ctgccattgt cgctacggct gtccattttg gtgtttttta cttcttaccc
     3361 tatctgcata ccaagtttac tgttgacttt ggatggttca acaagtatat agagggtgat
     3421 gtaaggaatc cagcaattgc agcatcaacc gcaattgttc tatcaatagc tgtaggcttt
     3481 ggccttcatt tcatttggaa gttaaaacct gcaaaaatcg atgtagcaaa ctcctaaacg
     3541 cataattcaa tggatattga gtcagtttat aaaagtaagc taattaacgc cgagcaggta
     3601 gccgatctaa ttgagagcga taccgacata atagtagctc agtgcgcttc agaaccgcaa
     3661 gggtgtatgg ggaaattcca tttggcaaag gaccgagttc acgatgtgaa ggtgttttct
     3721 gtgttaaccc ttaagcccta tgatttctat atgaaaccgg agatgaaagg acattttgaa
     3781 ctttgcagct ggttccatgc ccctggttcg cgccaggcgc ttaaagaagg aacaggtacg
     3841 gttacctatg tacctaacat gctgcataga gcagcaaccg ataggcttaa ggtccgcagg
     3901 ccccacatgt ttgttggaac atgtacccca cccgacgaga agggctacgt ttcgctttcc
     3961 ctaggcgtaa catacgaaaa ggatatattt gatgtagcag aactcaaaat tctggaggta
     4021 aacaagcacc tgcccagaac ttttggcgat actcatgtac acataagcga ggtgaattac
     4081 tttgttgaac accaccaaat tcctccaacg ctcccctcgc cacaacccga cgaggaagcc
     4141 atgaaaattg gtgagcaagt agccaagctg gtggaagatg gggctaccat tcagctagga
     4201 ataggtgaaa tacccaatgc tgttgctctt tctcttaagg ggaaaaagga tttgggagta
     4261 cataccgaga tgatggttga ctccgtaatg gagctatacg agcagggcgt aataacgaac
     4321 aagaagaaag cgctgcataa aggtaaattc atctgtacat ttgccatggg ttcagagaag
     4381 ctatacaggt ggctcgacaa taatccctcg gttgagtttc tgaagggtag ctacgttaat
     4441 gatccctgcg taattcgcaa aaactcaaaa atggtttccc ttaacacctg cattatgatt
     4501 gattttacag gacaggtggc gagcgaatct attggtattg agcagtactc aggaacagga
     4561 ggtcaaaccg atactgctga gggtgctgtt gagggattcg atggcctggg caagtcaatc
     4621 attgcctgta ggtcaaccgc taaaaggggg caaatatcta ccattgtacc ggttttaccc
     4681 gagggtactg gggtaaccct tcaccgaagc catactgatt acgtggttac tgaatgggga
     4741 cacgcaaggc ttaccggact caccgtcagg gaacgcacca aagttctcat atccattgcc
     4801 catccaaaat tcagggatga gcttacagca aaggcaaaac aaatgggata cctttattaa
     4861 ttaataaata aaacataaca tgataccagt cagaattaaa agtacaggag tttatgcgcc
     4921 cggcttgcca atcggaaacc aggagctaaa gaacctggca aaaattgatt ttgatacggc
     4981 taagaccgag agcaaaattg gaattgtaca gcggcatatt gcccatctgc ggggtataaa
     5041 cgaaactgca gccgattttg ccacaaaagc aggagttgag gcgatagcca atgcaggttt
     5101 agccgctagc gatattggac tcttcattgt ggcaaccgat acgccagagt acatttcacc
     5161 ctcaaccgca attgtagttc agggaagaat tcaaaaggga gaagtatggg gtggagcttt
     5221 cgatataaac gctgcatgtg ccagcttttc aattgcattc gatactgcaa gtcgaatact
     5281 tgcaaacaat agcctgattc gctttgctct agtcataggg gtatataata tgcctgcatt
     5341 tttcaggccg ggcgatgctt ttgggtattc aatatttgcc gatggtgctg gtgcatttgt
     5401 cctggaaagg gttgagggaa cagaatcttc ctcgggttac atcggcgggc agcagctaac
     5461 cgatggaacg cagtggaact acatcggaat ttattcagga gcagcacgca aacctattac
     5521 ccatcagatg ctcgactccg gagagtacgg ccttcagaac ctccaaccac ttcctggcga
     5581 ccgcaatgta aggctgtggc ctatggtggt ggagaagcta ctaaacggcc actcggttaa
     5641 acttgaggag gttgaccatt ttgttttcac ccagatcaac cactccgtaa ttgttaaggt
     5701 aatggaggtg ttgaaacaac ccatgactaa aactacctgt gttatggata gatatggcta
     5761 cacgggttct ggttgcctcc caatggcttt tcatcatgca gtttccgaag gaagggttaa
     5821 acgaggtaac aaggttgtat ttgttgcctc tggtgcaggt ttagctgttg gaagcaacct
     5881 attcatctac taatcgctca agccaatgaa taatcacgta gggattgttg gaagcggagt
     5941 gtacatacca gaaagaagaa tgaccgctcg tgaaatatcg gaggcaacca gcggggtatg
     6001 gtctgagaaa gccgttgtgg aaaaattagg catcaggcaa atagtaatac ccggccctaa
     6061 tgatggcact caggaaatgg gggtaaaagc agcccttgac gctttaaaaa atacatcaac
     6121 tgacccaaaa tctctcgatc tgatactttg tatgggagaa gagtggaagg agtaccccct
     6181 tacaacctcc ggcatataca ttcaggaaca gattggagcc acgaatgcct gggctattga
     6241 cgtccagcag cgatgtggaa ccacggtggc ctcaatgaaa attgccaagg atataatgct
     6301 ctccgatcct gacataagca cagtcttaat agttgggggc taccgcaacg gcgactttgt
     6361 taacttcaaa gattcctcgg tttcctttat gtataacctt tcggctggtg ctggtgccct
     6421 tatcctcaaa cgaaactatg gtcgaaactt acttctgggg acccacatca taaccgatgg
     6481 atcgctggcg cgggatgttg gcgttgaatt tggaggaact gccaatccta tcacccatga
     6541 taatgttcat gaagcctaca aatcgcttcg cgtattcaac gaacagcaca tgaagagtag
     6601 gcttaacgat gtttccatga gcaactggat gaagtgcata gacatggctt ttaaaaaatc
     6661 aggagttgat agagataagc tgggatacct ggctgggctg catttcaaac gctccatgta
     6721 cgagtctatg gttcatgaat tagggctaac tatggagcaa acaatttacc ttgaagaatt
     6781 tggccacatg gggcaagttg accaaattct atcacttcac cttggcctgc agctcggtaa
     6841 aattaagaat gggactatta tttcgatgat tgcagcagga attgggtacg cctggggagc
     6901 caatgttata cgatggggat aataggtcat accattaaaa gagatttata ataatctgta
     6961 tttgtgatat ttaaattgcc tacaaatata ttttagtttt aatgtcaacc ctttatatgt
     7021 agtttttcat ttgactcaag ttcattttta ttaattaatt gctaacaaat aaacacaatt
     7081 tcggtaatgt taacttcaaa actaaaacta tgagaaagta tcttgcaatt gcactcctgt
     7141 tttcgcttgc tctggtagct tgcgataaaa gtgatgagga agaaaagtat gatcctgcaa
     7201 aagtgggtat tgttggcgaa tggtactctg caggtagcga tgttgctgta ttgctgagca
     7261 cgtactttgc tgttgactcc atttacgcca agtttaacga caacaacacc tacacggtgg
     7321 aatcgtacag ccagggcgct aaaacaacca tgacgggaac ctatgtacag gcaaaatcca
     7381 acgtgggttc aatttggacc attaccctca atcaaaccac acctaccgcg ctaaccagtg
     7441 agggaatttt cgaaatcacc cttacctcgg gtaagtatgt gatgcgctac gaggtggttc
     7501 aaacctctcc caatattggg gccacacctc caactccggc tgcaggcttt ggcagctcga
     7561 atggtggtgc gctgcaaacc atgaacattc aaacctatag aatgctggtt cgttaaaaaa
     7621 tcaaaccaga aaggtcagaa aagcaatgct gttttctgac ctttcttgat tagcctaaaa
     7681 tttagcggaa atgaaagaag caagacacct aataacggca tgttttatca ttgccattta
     7741 ttcagcctca gctcaaaatc cttcaaacca gtattttaga ggtgagatac ccgaaaaggc
     7801 aaataaagag tttcagttta tggccttttt tattaaccag ggcgtaacat cgaacatata
     7861 cgctcaaaat gatttcctaa aaggacaggt tgttgggcgt ttgttcgggg gaaacacaac
     7921 ccgcacctcc gattctttaa cctccgccta cttggaacaa cgcatcattc cattctttat
     7981 ctaccaacca aaactgttca atggcaaggc aactcttagg gcatcattcg agatagattg
     8041 gacatggggc gatgtaagct acggtacagg tggtaacttt ggttcggcgg tttcgggcga
     8101 ccaggtaaac cttcagacac aaaacattga gctggagcta aatcccgcaa agggatgggc
     8161 catcaaccta gggttgcagc gaatgttcga ttctccctac aacccctaca aggccgcagt
     8221 tgataaaatg ggagtaaccg gttaccggtt agcttactgg ggtaccgatg ccgttggtgt
     8281 gtcggttcgt aaggattggg actactcgcg ggcaaaagtt ggcatgtata agctatacga
     8341 aaactacatt caggatgacg acgacgtaac catgtacgag ctaatgggcg agcgggatgt
     8401 atccagattg tggaaggttg gagcctcggt gaactacgtt cgcgataggg catctggcgc
     8461 tggtggggtt tccatactag gacaaggact taacagcttg ctggcctccc ataatggcgt
     8521 ttacaggttt aactttggca atgtccccta caaggccgat attttctgga tgggtgcttt
     8581 tttcggacga aataccgagc actggcttga tccatggatg gtaactggtt ttgttaattt
     8641 caacatcgga accgccgata cccttctcaa ccagaatgta tccctttcga aatggaagaa
     8701 atcaaccgat atttttggct ttgcaggtaa tctcaggttt tcataccgct acggacaaac
     8761 ccttaacgat aacgttaccc ttgatgttat ttactcttcg ggcgacggcg atggcctaac
     8821 cgatggacag tactccggag tcattaccgg gaaccagtgg ggtgctcccg ctgccatttt
     8881 cattacttcc ggatcgtaca tcctgatgcc ccatggcaac gtggtaaacc gattttcacc
     8941 cgctataatg gatatttcaa atatgggata tggccttaca gcggctactc ttaacttttc
     9001 cagaggcatc ataccacaca ggcttaacgc taaggtaggt tctgctattg ccttcagcaa
     9061 cattcaacct gccggtgggg gttactacat gggaactgag attaatggga atgtagccta
     9121 caactttgga ccctttatga gcctggagct tcatggtgca tacctgatgc tgggcgattt
     9181 ctacgatagc aagcaagctg cttacggtag ccctgtaaat ggtggacttg ataaacgacc
     9241 agtaaaccca tggactgtat ttatggtttt taaatggctg ctattttgat agtagcatga
     9301 aaagagccat ctatggatta tgccaaaaca tcaagaagta gcaggtgtat ttccacctaa
     9361 aatggtaatc cgtctattta actttttgca tttcaacgag aaatcaattc taatcacatg
     9421 aaacatgttg ttacatactg tctagtggcc gttatggtat ttacaggctg cagcacaccc
     9481 tacaagtcgt taaaaagcat ttcgtcaatg gacgaaatca cctacaagtt tcctgttaaa
     9541 aaggttatgc taccaaaaag tgggtacgaa atagcttata acgatgaggg cagcggaagt
     9601 aaaactatca tttttatcca cggagtaggt agctacctga gagcattcga aaagaatgtt
     9661 gaggttctta gagaaaacta ccgctgcata gccattgacc tgccaggcta cggaaaatcg
     9721 agtaagcaac cccatagcgg tatgatgtcg ttctatgcgg gtataattaa tgagctagtt
     9781 gaacagctaa accttggcaa ggtggtgata gccggacact ccatgggagg tcaaatagct
     9841 atgactacag ccctcctata tcctgatata gttgaatcat tagtgctggt tgacccagca
     9901 ggttttgagc gttttcacaa aggtcaaaaa caatggttta gagatgtgat gacctttgaa
     9961 ggtgttaagt acacaacacc cgaaggcatt cagaacaacc tggcaaccaa tttttacagg
    10021 gttccaaagg atgccgaatt catgattacc gacagaattc agatgcgaac agcttccgat
    10081 tttgatgcct actgctacgc agttgtgcaa tccactattg gaatggttga cgaaccggtt
    10141 attgattacc taaaagacat ctccgttcca accctgatac tttttgctga aaccgataac
    10201 ctcattccca accggtatct taatccgggt cgtaccattg atattgcaaa atctggcgct
    10261 tcccagattc ctaacagtaa gctggttatg gttcctaaat gcggtcactt catgatgttt
    10321 gaaaaacccg acgtttttaa tgcgaatgtg ttggagttct tgaaataaaa tttagggtga
    10381 aatggaagcc gtggtgatag gtgaacattg caaggtaagc tacattgagg aagtcccatg
    10441 catcatgatt caatggtttg gtttgccccc aaccgaagaa tttcgaatgg gctgtaacac
    10501 cgtgttggag atgatgaaaa aatactctgc aaccaaggtc ttgaccgata actcccatgt
    10561 taaggtattt tcagttacgg atcagaaatg gctaaatgaa acatggttac ccaaagccga
    10621 agcgcttggc taccgcgtat ctgcaacatt aatcgcagag ggggaccctt ttgttacctt
    10681 tgctgtaaat aatatcatgg caaatcgcaa ccccaataaa tttaaagccc gctattttac
    10741 caagtatgac gaagccatac agtggctgaa agaatgttag caacccgatt tctcgttcca
    10801 gaaataaccg gctttgagcc ggtttttttt actaaattgc cctattaatt gtaccgaaaa
    10861 agatgaagga taagctggca acttttcaaa gtctctgtga tgagctttac ccccatgagc
    10921 tcgattacct gctctctatc aaccagttcc agaaagggga gaacgtccag gtactccaac
    10981 ttgtgtacta caacgccaca aacccggata atcctatacc attcgacaaa tcgttggata
    11041 aaagaaccta ttcctatatc aaatcatgga ttctggataa ccttcaaaaa attgacgtgg
    11101 atgtattcta cgattgggtc agcggtgtgg aaaggattat acttaacgac tccattcaaa
    11161 gcgccgatga aaaacagctg ctaaaaatga tggaggtaat taagcctacc gactactact
    11221 ttatgaagtt tttccgagtt atccagcatt atcgcgatta cctgctggta cgcaaccgcc
    11281 cgcgtaacta tgagctagta accgactttc tggcaaggag taagtcgagc tacctttatt
    11341 cccaatcgct caacaacaag atgaacgaag ctgccgaaaa ggtaatctat cggaaaatat
    11401 gggttcctga agaatttaac gtttgggaga aacttttctc cgacatctac tacaacgaaa
    11461 ctattgatgg ctacactcgc tacagagcag tggttaggct tactatacta tactacacac
    11521 aacgggaatt cgacaagctt aagctggttt accagcatct cgaccaacag tttaaaaccc
    11581 cggtgttcta ctccaagcga attctggcta actactatgc caacagggct atgatgcact
    11641 ccaagctcaa cgagctggag gaggctgaaa agtttggtat gctctccatt cgacaccgaa
    11701 acagcgatta ccttttttac ctggtaaatg tatgtgatgt gctgctaaga gccaataagg
    11761 cacctaaggc cttaaaactg ctggtgaact ctttccccga tctaaaaacc tcaggtaact
    11821 tctactataa aattggtttt gcctcattgt atattaaaac attggttgcc aacaacatga
    11881 ctaaaaatgc ggtggactac ggggaaattt ttctgaatgg ttataaagat gaaatttttg
    11941 aataccgctg gcacttgttt ttctcgtcct atattggtgc cctctttcgt tttgaaaagt
    12001 actcaaaaat tctggcctta ggcaagcgct ataagctatg cgcccaggaa cgtcagctgc
    12061 tgggtaccgc tcaatatctc cctctaattc aactttacat gctagcttcg gagtatatgg
    12121 agggagtaac tacaaaagat aagcttcggc aagcgataat gcagcatatt caggaggtgg
    12181 gacaaaatag atttcgccat agaaaagttc tcgacacgct caaaacgctc tcattgtgca
    12241 ttcccgatgt catttcaggc atcatggtaa ccaatcaaat tcatgaggag atttagctta
    12301 ccagcaaaat caacgtgtca accccagctc ccgacaattg cggtatattt aaactcactg
    12361 ggtactatgc ttaacttgta ataacatcat tttagctttg atactgtccc taaaagagtt
    12421 gaacattcag ctttggggaa gatgaattac atgtaaaact agtttt
//
LOCUS       DAOSHM010000064        12462 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_4254_length_12462_cov_21.099218, whole genome shotgun
            sequence.
ACCESSION   DAOSHM010000064 DAOSHM010000000
VERSION     DAOSHM010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 12462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12462
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4254_length_12462_cov_21.099218"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            557..2617
                     /locus_tag="PK839_08865"
     CDS             557..2617
                     /locus_tag="PK839_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005928401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOG72885.1"
                     /translation="MITRELINPQSIVVVGGSADVQKPGGKVLKNLIDHNFKGKLYVV
                     NPKADEVQGVRAYPDAEDLPPVELAILAIAAKFCPQAVETLAAKKGTKAFIILSAGFH
                     EESEEGARLEQQIVETINKAGGCLIGPNCIGVMNTNYAGVFTTPIPRFNTHGADFITG
                     SGATAVFIMESGISNGLQFNSVYSVGNSAQLGVEDILEYLDLTFDPNTSSRVKMLYIE
                     SIGQPQKLLKHASSLVRKGCRIAAVKAGSSSAGSRAASSHTGALASSDVAVDALFRKA
                     GIVRCYGREELTTVASIFMHPRLTGKNIAVITHAGGPAVMLTDALSNNGLEVPPISGS
                     KANELLSKLFPGSSVANPIDFLATGTAEQLGYIIDACENDFSHIDAMAVIFGSPGLFP
                     VYDVYDLLDKKMSECHKPIFPILTSVINVRDEINQFIAKGRVNFPDEVVFGNALTKVY
                     NTPDPAPEDPKLPIIDTSRIRRVIDGCSNGYLQPNQVQELLDAAGIPRAGEAIVKTAT
                     DATSAAMKMGFPVVMKVVGPIHKTDVGGVVLDVRDEQMVTREFNRMMGIKDTTAVLIQ
                     PMLKGLELFVGAKREGLFGHVVLCGLGGIFIEVLKDVKAGIAPIEANEAHSMIKHLQG
                     YGMIKGARGQEPVNENLFADIISRVSVLVGVAPEIFEMDINPLLGTKDKVVAVDARIR
                     IEKK"
     gene            2629..2781
                     /locus_tag="PK839_08870"
     CDS             2629..2781
                     /locus_tag="PK839_08870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72886.1"
                     /translation="MSIKGVFKKLSKNEKILLVIIILLLIMVATQWEKIWNEAIRAFG
                     RYFGSK"
     gene            complement(2958..3743)
                     /locus_tag="PK839_08875"
     CDS             complement(2958..3743)
                     /locus_tag="PK839_08875"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HOG72887.1"
                     /translation="MKKHLHIPLLLLMLASCSEDKSVFPTLTQDVCIGWRIDSAITAN
                     PAQFNVLERSKYAAIYSYTEEILQQLLESDGVRNAEAFSWQIRVINDKSVISAFVAPG
                     GYIYLYTGILMALDNEAQLAGLLAHQLAHIDCRHITKMLLRHYDLSTFESIAAGLEQA
                     ELDSIIHGLVNGSSTLKYSCSNEHEADSKAMEYLFDTGYDPYELINMYRKLLTCSVVA
                     QFFAIHPWDDGRIENLEAAWKNLGARHGEKYTERYLDFKKSLP"
     gene            complement(3801..5006)
                     /locus_tag="PK839_08880"
     CDS             complement(3801..5006)
                     /locus_tag="PK839_08880"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HOG72888.1"
                     /translation="MENESKSKLVIGILTGGGDVPGLNPAIRAITIRALREGYKVIGF
                     RRGWAGLIEVIRENDADNSKCYEVLTEERVNKAFRTGGTFLHTSRTNPSKVSKDSVPD
                     NLKSKYHKEVNDLTPEVLKNLAFLGIDYLIPIGGDDTLSYGVRLYQEGFKVIAIPKTM
                     DNDVPGTDYCIGFSTCITRTMSLTDMLKTSAGSHERILVMEVFGRYAGFTAMLPTMAG
                     VANRCVIPEYKFSIERLTELLISDRNLNSSKYSTVLVSEGATFEGMEQMVFQSAERDA
                     YGHAKLGGIGDVVSRMIKEISPKFNNGKSVNVITQNLGYLVRGGEPDAIDSIVPMAFG
                     NLALDLIIKGIHGRLVVLKNGRYDDVPIDVVISQKKVVNVSKYYNTERLRPFYKSFEM
                     QPLFIMTSD"
     gene            5316..5534
                     /locus_tag="PK839_08885"
                     /pseudo
     CDS             5316..5534
                     /locus_tag="PK839_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013617848.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
     gene            5890..7191
                     /locus_tag="PK839_08890"
     CDS             5890..7191
                     /locus_tag="PK839_08890"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOG72889.1"
                     /translation="MNNKRLQPYAVYSDGKGNIYEDRSLFAVGRSGHEFYPLYLDEMI
                     PLPEGSDLFELPGRKTVGYSRNGKLTARQSGLAAAAFIAPAHTQLSVAAWENKVNAPV
                     LPLFAYTAVGWYANRFYVPAIRIDLDTRQDCENFDQQVVVERGKALLEQHEGNRLVNH
                     LVENCAFTYLCPAARNMVMERWEAPIPVSPTCNARCIGCISKQDQQESPIPETQHRLT
                     FVPTVGEIAGYAVPHLENAPNAVVSFGQGCEGEPLMQWELIRDAIKLIRSKTQKGIVN
                     LNTNGSKPRAVEELFKAGLDSIRVSMNSAQPKWYESYFRPVGFSQNDAIESLTIASRY
                     NRWASINYFVMPGFTDTAEELTALKNLIEKTDLNMIQWRNLNIDPDWLFGKTNFTGNS
                     KPLGIRYVMDEIHRSYPSVYFGYFNPHYEVMHRWQQQTGNS"
     gene            complement(7341..7496)
                     /locus_tag="PK839_08895"
     CDS             complement(7341..7496)
                     /locus_tag="PK839_08895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72890.1"
                     /translation="MSSKDIRVIFRLHRKVWYAWCLSTFPGVELKINRVAINTAGSYQ
                     NPLYLKK"
     gene            7553..9049
                     /locus_tag="PK839_08900"
     CDS             7553..9049
                     /locus_tag="PK839_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HOG72891.1"
                     /translation="MGSIQLELALLILSTLFFLSILAGKVSSKYGVPALLLFLIVGML
                     SGSDGLGIQFENIHISQAIGTIALCVILFSGGLDTQFSEIKPVLAQGLILATLGVLLT
                     AIFTGLIIWWILDLTIPGANIGLLTSLLLAATMSSTDSASVFSILRSKGLNLKKNLRP
                     MLELESGGNDPMAYVLVITLIDIIKMNSEPNFWLASGMIVLQLIIGGGVGYLLGKLAI
                     ITINHIKLGNDSLYPILVFTFCIFIFSITYFLRGNGFLAVYVGGLVIGNAKFVHKRSS
                     LNFFDGLAWLFQLVMFLTLGLLVNPHELIPIILPALAVSIIIIFITRPISVFICLLPF
                     RKLSFKDKSFVSWVGLRGAVPIIFAIYPLVENVPHARIIFNIVFFCTLISLLVQGTSI
                     PLVAKWIGVIEKPERFSKLKPFNMDFADNLKTVSTEIEITDKMLLRGNQIMNLELPDN
                     TLVVMVKRGDNYFLPSGSTTLKEKDKLLIITDNQEALVETYRNLGIDG"
     gene            9245..10837
                     /locus_tag="PK839_08905"
     CDS             9245..10837
                     /locus_tag="PK839_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008155270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOG72892.1"
                     /translation="MKRIITLLFVLAGLMAHSHAQSDSVIQKIIQIGTAENQTMKHLD
                     VLCNQIGGRPVGSSAYEDAVVWAARQFQAWGMDVFLDEVDELPVGFNRGPWFGRLLGD
                     HSMTLHFATPSYTAGTKGVQRGHVVAEPKTQREFERMKGKLKGAWVLISGESNGWPID
                     YSVRANHRRDSIIASNNEIEKKNDEIQRENWMNRSANQPPKELIPLTEEPGLFYRQMV
                     EAGILGIIQSAPNPIRVLYDRKNINSMVWDSLPQVPDIKLDENQYKIIERMARERQYF
                     QLEFDIRNHFRPGPIPYHTVVGVIKGTEFPNEYVIMGGHLDAFDVATGAADNASGVAP
                     AMEAARLIMKAGGKPKRTILVCLWAGEEFGLLGSTSWVIKNSDKLQSISAMFNRDGGP
                     TVASNLSVPKAMLNDFSGICSPLNSINPSFPFTLKEATPRPKPKNPGGTDTAPFLMAG
                     VPAFQFGTEDVDGLDFSYQEIWHTERDTYEKCPADYMNHTSIVTAIVVYGVANLDHLL
                     SRENLFAPDDKPKVNGKAKGKK"
     gene            11012..12442
                     /locus_tag="PK839_08910"
     CDS             11012..12442
                     /locus_tag="PK839_08910"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOG72893.1"
                     /translation="MAVGCTLNAAAQEFDSVIIKQHVYTLASDSLMGRGFGTTGGKMA
                     ADYITAQLEAAGVMPWQGSYLHPFISSGIMVKTEGANIIGWVEGTDPLLKDEYIVIGA
                     HYDHLAYKITDDGKRIVFNGADDNASGVAAIIEIGRWLAGSRNQLKRSVILAAFDGEE
                     AGLVGSSFMVQHNQVPPKKVKVMFSLDMVGMLSRYGGIDLNGDKTLENGDVMLHNLAS
                     KHGLTVKKDGKNVVPNTDTAPFGNQGIPAIHIFTSTVSPYHKPEDDAEKLDYPGIAAI
                     SGFVADAAVQLSNAETLEPSRRFVAQQNTKLPKFGLRLGFGTSGQNYSNEFYMAKNIF
                     AFQAGFYAKQRLNRRFALQPEIIYQTMGSKHTDGTLRTHEVSIPVNIRLSLSPASSAS
                     TFISNDFYIFTGPYYSYRFGGGLGGKDMDFDNTFRREDYGIQGGFGFEIMNFQLQISR
                     FRSFRSIDRTTTIMPYGMMWTLGLRF"
BASE COUNT         3560 a         2840 c         2799 g         3263 t
ORIGIN      
        1 aatgagtaaa actataaagt tccaccgcgc tgccggtgtt tttccctcgc tgttaaaaat
       61 gaatgggatc ctgatggctt tccccttttg ctacatgcct aattgaagac atggcggggt
      121 gagagatggc tgtgcgtaaa aaccccattt caggctgtcg gaaaatgcct gccctgtagc
      181 aagcgtatcg ggagtttatg ctgaaacccg aaatttttta gcaaaatcct tgcatgttta
      241 aaagcataat tttatcttct gcaacaaaat aaaagtccgg gttttcatga acaaccctgt
      301 gaattgccat gcaatcagga agaaattaaa attaaaggta atatgagtaa acttggcagc
      361 tagtaaagtt ttgaccttaa aaagttgaac aatagtgaaa ttgactggct gaaatcgcaa
      421 accaataaaa tggcatagtt cgtagtccat ttttataggt tctcactttc aaattgaagc
      481 ttgctacatt gctttattag cattacgttt gcaaccggaa aaatatcctg aaaaaacagg
      541 tttaaaatac ttttttatga taaccagaga gctgattaac ccccagagta tagttgtagt
      601 gggtggttct gccgacgttc agaaacccgg tggtaaggtt ttgaaaaacc ttattgacca
      661 taattttaag ggtaagctat acgtagtaaa ccccaaggct gatgaggttc agggtgtcag
      721 ggcatatcct gacgccgaag atttaccacc tgttgaactt gccattctgg ccatcgcagc
      781 aaagttctgc ccacaggcag tagaaacgct ggctgcaaaa aaggggacaa aagcatttat
      841 catcctatca gccgggtttc atgaggaaag cgaggagggt gccaggctgg agcagcaaat
      901 agttgaaacg ataaacaaag ctggtggatg tttgattgga cccaactgca ttggggtaat
      961 gaataccaac tatgccggtg tttttactac tcctattcca cgctttaata cgcatggcgc
     1021 agatttcatt accggttcgg gagcaacggc cgtatttatc atggaatcag gaatttccaa
     1081 tggacttcag ttcaacagcg tttactcggt tggcaacagc gcccagctgg gagttgagga
     1141 cattctggag tacctggacc taaccttcga tccaaacacc agctccagag taaaaatgct
     1201 ttacattgaa agcataggcc aaccccaaaa acttcttaaa catgcctcat cgcttgtgag
     1261 aaagggttgc cggattgcag ctgttaaggc aggaagctca tcagcaggaa gccgggctgc
     1321 ttcaagccac accggagcac tcgccagcag cgatgtggct gttgatgcac tatttagaaa
     1381 agcgggtatt gttaggtgct acggacggga agagctcaca accgttgcct caatattcat
     1441 gcatccaagg ttaaccggaa agaatattgc agtaataacc catgcgggtg gcccggcggt
     1501 aatgcttacc gatgccctat cgaacaacgg gcttgaggtt ccccctatta gcggttcaaa
     1561 agctaacgaa ctactctcca agctttttcc cggttcatcg gtagccaacc caattgattt
     1621 cctggcaacc ggcacggccg agcagctggg atacataatt gatgcatgtg aaaacgactt
     1681 tagccatatt gacgcaatgg cagtaatctt tggtagtccc ggattattcc ctgtttacga
     1741 tgtttacgac ctcctggata aaaaaatgtc tgaatgccac aagcccattt tccccatcct
     1801 tacctcggta atcaacgtca gggatgagat taaccagttc atagcaaagg gcagagtaaa
     1861 cttccccgat gaagtagtgt ttggaaatgc ccttacaaag gtttacaaca caccggatcc
     1921 tgctcctgaa gaccccaagt tgccgataat agacaccagc aggattcgta gggtgattga
     1981 tggctgttcc aatggttacc tacaacccaa ccaggttcag gagctactcg atgctgccgg
     2041 gattccccga gctggcgaag ccattgtaaa aacagccaca gatgcaactt ctgcagcaat
     2101 gaaaatggga tttcctgtgg taatgaaggt tgtaggacct attcataaaa cagatgtggg
     2161 aggagttgtg ctggatgttc gcgatgagca aatggttacc agggagttta acaggatgat
     2221 gggaattaag gataccactg ctgtgctcat tcaacccatg ctcaagggtt tagaactgtt
     2281 tgtaggagcc aagcgcgagg gtttgtttgg ccacgtcgtt ctgtgcgggc ttggcggtat
     2341 ttttattgaa gtactaaagg acgtgaaggc cggcattgct ccaattgaag ccaacgaggc
     2401 tcattccatg attaaacacc tgcagggcta tggaatgatt aaaggggcta ggggccaaga
     2461 gcctgtaaac gaaaacttgt ttgccgacat catcagccgg gtttcggtgt tggtgggtgt
     2521 ggcgcccgaa atatttgaaa tggacatcaa ccccttgctc ggaacaaagg acaaagtggt
     2581 agctgttgat gcgcgaatca gaatagaaaa gaaatagcct agctggtaat gagcataaag
     2641 ggtgttttta aaaaactttc caaaaacgaa aaaatactac tcgtcatcat tatccttttg
     2701 ctgattatgg tagctaccca atgggaaaaa atctggaacg aggctatcag agcgtttggc
     2761 aggtactttg gcagcaagta aaccttattc ttttaaggaa tggaaaagcc tagttgtggg
     2821 ggcattttca catttacttt catcgggaaa atcgtgagtg gtgcgattac cccttttttc
     2881 aatacaagct gggttttaaa gtctatacca aatgagtttg atttgcccct ttagaagggg
     2941 ctaaaataca ggaatcatca aggtaaagat tttttaaaat ccaggtagcg ttctgtatat
     3001 ttttcgccat gcctggcacc cagatttttc cacgcagctt ccaagttttc tatcctccca
     3061 tcatcccatg ggtgaatagc aaagaattga gcaacaacag agcatgtaag tagcttcctg
     3121 tacatgttga taagctcata gggatcatag cctgtatcga acaggtattc catggcctta
     3181 ctatcggctt catgttcgtt gctgcagctg tattttaggg tagagctccc gttgaccaac
     3241 ccatggatta tactatcgag ttcagcttgt tcgagaccag cagcaatact ttcaaacgtt
     3301 gataaatcat aatgccttaa aagcatcttg gttatatgcc ggcaatcaat gtgggccagc
     3361 tggtgagcca gtagccctgc cagctgagct tcgttatcaa gagccattaa tatgcccgtg
     3421 tagaggtaga tgtaacctcc cggagcaaca aatgcactga taaccgattt atcatttatc
     3481 accctgattt gccagctaaa agcttcagca ttgcgtacgc cgtccgattc caaaagctgc
     3541 tgcaggattt cttcggtata gctgtaaatg gcagcatact tactacgctc cagcacgttg
     3601 aattgcgctg gattggcggt aatggcagag tcaattctcc aaccaataca aacatcctgc
     3661 gttagggtag gaaaaacgga cttatcctcc gagcaggaag caagcatcag cagcaataat
     3721 ggaatatgca aatgcttttt catcaggaca taatgaagcc aaacctaccg tagattaaaa
     3781 tatgcagctc aaagtggcgg ctagtcgctg gtcatgatga agagtggctg catttcaaag
     3841 ctcttataga aaggccttag cctttcggta ttgtagtact ttgaaacgtt cacaaccttt
     3901 ttctggctaa tcacaacgtc gataggcaca tcgtcgtagc ggccgttttt aagcacaacc
     3961 aggcgaccat gaatcccctt gatgatgaga tcgagggcaa ggtttccaaa ggccatggga
     4021 acaatagaat caatagcatc gggctctccc ccacgaacaa ggtatccgag attctgggtg
     4081 atcacgttta ccgacttccc gttattaaat ttaggagata tctccttaat catcctcgaa
     4141 acgacatctc caattccccc aagcttggca tggccgtatg catctctttc ggcgctttga
     4201 aaaaccattt gctccattcc ttcaaaggtt gccccttcgg agaccagcac agtggagtac
     4261 ttgcttgagt taagattgcg atcgctaata agcagttcgg tcagcctctc gatgctaaac
     4321 ttatactcag gtataacaca cctgttggcg actcccgcca tggttggcag catggcggta
     4381 aacccggcat agcgtccaaa aacctccatt accagaatac gctcatgaga gccggctgaa
     4441 gttttgagca tatcggtcag gctcatggtg cgggttatgc aggtgctaaa acctatgcag
     4501 taatcggtac caggcacatc gttatccatt gttttcggaa tggcaattac cttaaaaccc
     4561 tcctggtata gccgaactcc atagctaagg gtatcgtctc ctccaatagg gataaggtag
     4621 tcaatcccta aaaaggccag gtttttcaga acctcgggag taagatcatt tacctccttg
     4681 tggtacttgc ttttcaggtt atcaggaaca ctatccttgg aaaccttgct tgggtttgtc
     4741 cgcgaggtat gtagaaaagt tccccccgtt ctaaaagctt tgttaacccg ctcctcggtt
     4801 agcacttcat agcacttgct gttgtcggca tcattttcac ggataacctc tattaatcct
     4861 gcccagcccc tgcggaagcc tattacctta tatccttcac gtagcgccct gatagtgatg
     4921 gcacgaatgg cgggatttaa tcccgggacg tctcctcccc cagtgagaat tccaatgacc
     4981 agcttagatt ttgattcgtt ttccatgatt ttcaattggt ttatgacggg tcaattatca
     5041 aatacctagc aaatatatgt tttatatcat aaatttcatt ggtttaccaa tgttaaattt
     5101 tcaccaccca caacataaac ccatttccaa aataatcggg atggtagtat attcaactac
     5161 aaatgcttct ccacaagaaa aaattaccaa aacggttagc accggtcata cccatcccta
     5221 atgttaatta gccagatatt acggcataac ctactataga ttgaatatca ataacttata
     5281 aataattgag tatgtagggt gattattccc cgccctctca cacctccctt ttcagcctga
     5341 ttactttgcc tgttgagttc gatgcatcgc gtagggcgct ccagtggcta ggcttaaccg
     5401 gaattcagta cggtcaaaat gtagtttacg ccagtgatgc actaaaatgg gcagccctca
     5461 cgtatgttgt tgccgcctta ggctcgttgg caaccctttt cttctacctt gggtttctaa
     5521 acaataacga ctaggagaag caccaaggaa actcaactaa acatagttct aataaaaaag
     5581 gtcggcatat aaccggcctt ttttatttat gatagataat caggcttaca ttatggcata
     5641 aaattgatcc ttagccgttc cacaaagctc gcaatttgcc ttcacatcat ttttatcata
     5701 caataatcat aatgcattaa gcaacaagca caaacactct cagctacagc tgaacaaagt
     5761 atgtgttgaa agcttttgtt tgaggaagtt acttacctta aagcatggaa gcataaataa
     5821 atgatttttt aaccaaaatt ttacggcttg cgaaaagagg ggtattttta caggcaataa
     5881 ataaagaaaa tgaacaacaa gcgacttcaa ccctacgctg tttacagtga tgggaaggga
     5941 aatatttatg aagataggag cctatttgca gtaggacgct ctggccatga gttctaccct
     6001 ctctacctcg acgaaatgat acctcttcca gagggtagtg atttgtttga actcccaggt
     6061 cgtaaaacgg tcggatatag ccgtaacggc aagctaacag ccaggcaaag tggattggcc
     6121 gcggcagctt ttatagcgcc agcacacact cagctttctg tggccgcatg ggaaaacaag
     6181 gttaatgcac ctgtactacc gctatttgcc tatacggcag ttggctggta tgcaaacagg
     6241 ttttacgttc ccgccattcg gatcgacctc gatacccgtc aggattgtga aaacttcgac
     6301 cagcaagtag tggttgaaag gggtaaggct ttgcttgagc aacacgaggg aaaccggctg
     6361 gtgaatcacc tggtagaaaa ctgcgctttc acctatctct gtcctgctgc acgcaacatg
     6421 gtgatggaac gttgggaggc accaattccg gtatctccca cctgcaacgc ccggtgcatt
     6481 ggctgcatat caaagcagga ccaacaggaa tcgcctattc ccgaaacaca gcacaggctc
     6541 acctttgttc caaccgttgg cgaaattgcg ggctatgctg ttccccattt ggaaaatgcc
     6601 ccaaacgccg ttgtgagctt tggtcagggt tgcgaaggcg aaccgctgat gcagtgggag
     6661 ctaatacgcg atgcgattaa gctcataaga agcaaaaccc aaaaaggcat tgtgaacctc
     6721 aacaccaacg gcagcaaacc ccgggcagta gaagaacttt tcaaagcagg gctggatagc
     6781 atacgggtaa gcatgaactc agctcagccc aaatggtacg aaagctactt ccgtcctgtt
     6841 ggctttagcc agaatgatgc cattgaatcg ctaacaattg ccagccggta caaccgttgg
     6901 gcatccatca actactttgt aatgcctggc tttaccgata ccgcagaaga gctaaccgcc
     6961 ctgaaaaacc tgattgaaaa aacggatctg aacatgattc aatggcgcaa cttaaacatt
     7021 gaccccgact ggctgtttgg taagaccaac tttacaggca acagcaaacc ccttggaatt
     7081 cggtatgtaa tggatgaaat ccatcgtagc tacccctcgg tttacttcgg atacttcaac
     7141 ccccattacg aggtcatgca tcggtggcaa cagcaaactg gtaatagtta agaacaatgc
     7201 aggtaaactt ttaagttaat gggatgattg attttagtag tacttctgag tatgggtaat
     7261 ttttgtattg aagttttaag taattctatt tactagaagt aaaacatttg aaccacatca
     7321 ccggaaaggg tactaaaaat ctacttcttc aagtataacg ggttttggta tgaacctgca
     7381 gtatttatgg caaccctgtt aatcttcaac tcaactccgg ggaatgtgct caagcaccag
     7441 gcataccata ctttcctgtg cagcctgaaa ataaccctga tatctttgct gctcatctta
     7501 cactgtgtaa aagttgctca aaaatgctat ctttatgggt taaaataaca aaatgggaag
     7561 cattcaactt gaactggcat tacttattct atccacacta ttttttctca gtattcttgc
     7621 tggaaaggtt agctcaaaat acggagttcc agccctgctt ttatttttaa tagttgggat
     7681 gcttagcggc agcgacgggt tgggtattca atttgaaaat atccacatct ctcaagcaat
     7741 agggacaata gcgctatgcg tcattctatt ctcaggagga ttggacacgc aattttctga
     7801 aataaaaccg gttcttgccc agggattaat acttgccaca cttggagtac tgttaacagc
     7861 aatatttact gggttaatta tttggtggat actcgattta accatacccg gggcaaacat
     7921 tggactacta acctctctcc tgctagcagc aacaatgtca tcgactgact ccgcttctgt
     7981 tttctccatt ctgcgttcaa aagggctcaa ccttaagaaa aacctccggc ccatgctgga
     8041 gttggaaagt ggtgggaacg atcccatggc ttatgttctg gtaattaccc tgatagatat
     8101 aatcaaaatg aactccgagc caaatttttg gctggcttca ggtatgatag ttttgcagct
     8161 gattattggt ggcggcgtgg gatatttgct gggtaagctg gcaattatca caattaacca
     8221 tatcaaattg ggtaatgact cactataccc aattttagtt tttacctttt gcatatttat
     8281 cttttcgata acctatttcc ttcgtggcaa cgggttcctt gccgtttacg ttggggggct
     8341 ggtaattgga aacgccaaat tcgttcacaa gcgttcttct ctaaatttct tcgacgggtt
     8401 ggcatggcta ttccaacttg tcatgtttct taccctggga ttgctggtaa atccgcatga
     8461 gcttattcca atcattttac cagctttagc cgtaagcatc atcataattt tcatcacccg
     8521 gccaatttcc gtatttattt gcctgctgcc attccgaaaa ttgtccttca aggataaatc
     8581 atttgtgtcg tgggtaggac tccgaggagc cgttcccatt atttttgcca tatacccttt
     8641 agttgaaaat gtaccccatg cccgaattat attcaacatc gtattcttct gtactctgat
     8701 ttcgctactg gttcagggta catccattcc actagttgcc aagtggatag gcgttatcga
     8761 aaaaccagaa aggtttagca agctaaaacc attcaacatg gactttgccg ataacctgaa
     8821 aaccgtttca accgagattg aaattacgga caaaatgctc ctgcgcggaa atcagataat
     8881 gaaccttgaa cttcccgata acacgctggt tgtaatggtg aaacgaggag acaactattt
     8941 tcttccttca ggcagtacaa cattaaagga aaaagataag cttttgatta ttacggataa
     9001 tcaagaagcc cttgttgaaa cgtatagaaa tttgggtatt gatgggtaaa aaatcaccag
     9061 caacccgagc tcctaaaccc gtataatggc atagccaatc gcggtaaata gtgctacctg
     9121 attttccgaa tatctatcct ggagaagcag tggtaaatcg gcaattaagt aaataaatgg
     9181 aatagccgct cgttaaacta tcagggagca aaaaattcta atgtgaaaat aaaatgttgt
     9241 tgccatgaaa cgaataatta cattgctttt tgttttagca ggattgatgg ctcattccca
     9301 tgcccagagt gattcggtta tacagaaaat catccaaatt ggaactgctg aaaaccaaac
     9361 catgaagcac cttgacgtcc tatgcaacca aataggcgga agaccggttg ggtcttcagc
     9421 gtatgaggat gccgttgttt gggctgcccg acaatttcag gcatggggta tggatgtttt
     9481 cctcgacgaa gtagacgagc ttcctgtagg gtttaaccgc gggccctggt ttggcaggct
     9541 gctcggagac cactccatga cacttcattt cgctactcca tcctacactg cggggacaaa
     9601 gggtgttcaa cgcggacatg ttgtagctga acctaaaacc caacgtgagt ttgaaaggat
     9661 gaaagggaag ctaaaaggtg catgggtgct gatttcaggc gaaagcaacg gttggccgat
     9721 agactactcg gttagggcaa accataggcg cgatagcata attgccagca acaacgagat
     9781 tgagaagaaa aacgatgaga ttcagcggga aaactggatg aaccgtagcg caaaccaacc
     9841 acccaaagag ctgattcctc ttaccgaaga accgggttta ttttacaggc aaatggtaga
     9901 ggcaggaata ctcggaataa tccaatctgc tcctaatcca atcagggtgc tctacgacag
     9961 aaagaatatt aacagcatgg tttgggatag cttgccccaa gttccagaca tcaagctgga
    10021 tgaaaatcag tataaaatta ttgagcggat ggccagggaa cgccagtact tccagctgga
    10081 atttgatatc cgcaaccatt ttcgtccagg ccctatcccc taccacaccg ttgtcggggt
    10141 tattaaggga accgaattcc ctaacgagta tgttataatg ggcggtcatc tggatgcttt
    10201 tgatgtggcc accggcgcag ccgataacgc ttcaggggta gcccctgcca tggaagccgc
    10261 aaggctaatc atgaaagcag gaggtaagcc caaacgcacc atcctggttt gcctctgggc
    10321 aggcgaagag tttggcctgc tgggttcaac cagctgggta atcaaaaaca gcgataagct
    10381 tcagtccatt tcagccatgt tcaaccgtga tggcggacca accgtggcaa gcaacctttc
    10441 ggttccaaaa gccatgctca acgacttttc cggaatttgt agcccattga acagcattaa
    10501 tcctagcttc ccattcaccc tcaaggaagc aacacctcgc ccaaagccta aaaatccagg
    10561 aggaacagat accgctccat tcctgatggc cggagtgcct gccttccaat ttggaacgga
    10621 agatgttgat gggctcgact tcagctacca ggaaatttgg cacaccgaac gcgacaccta
    10681 cgaaaaatgc cctgccgact acatgaacca cacttcaatt gtaacagcaa tagttgtgta
    10741 tggcgtagca aatctcgacc acctgctatc acgtgaaaat ctttttgccc ctgacgataa
    10801 gccaaaggtc aacggcaagg caaaggggaa aaaatagact gcgcatagcc gaagcatgtt
    10861 gttaaaatta cattaatcac ctacatttac accaggatgt ggtgcaggta tgctgtaaca
    10921 aaaatgcacc attaccgtct taacatcaaa ctttttagct ctaaccctaa gctaccaaac
    10981 caagatgaaa tcaaaattga ttttagcctt aatggcagta ggctgcaccc ttaacgctgc
    11041 cgctcaggag tttgattcgg tgataattaa acagcatgtt tacacccttg cttccgattc
    11101 gttaatgggg aggggttttg gaacaaccgg aggaaaaatg gcagccgatt acatcacagc
    11161 gcagctcgag gctgccggag ttatgccctg gcaggggagc tacctgcatc ctttcatcag
    11221 ctcgggaata atggtaaaaa ccgaaggggc taatattatt ggctgggttg agggcacaga
    11281 cccgctgctt aaagatgagt acattgtgat tggagcccac tacgaccatc tggcctacaa
    11341 aataacagac gatggtaaaa gaatagtctt caacggagcc gatgacaacg cctcgggcgt
    11401 agcagctata attgaaattg gaagatggct tgccggaagc aggaaccagc tgaaacgatc
    11461 ggtaatttta gctgcctttg acggagagga ggccggattg gttggatcga gttttatggt
    11521 acagcataat caggtaccac ccaaaaaggt aaaggtcatg ttcagccttg atatggtagg
    11581 aatgctttcc cgatatggag gcatagacct gaatggggat aaaacgcttg agaatgggga
    11641 tgtcatgctc cacaacctgg cctcaaaaca tggtttaacg gtaaaaaagg acggtaagaa
    11701 tgtcgtgcca aacaccgaca cggcaccttt tggtaaccaa ggtattccgg ccatccacat
    11761 attcacctca accgtatcgc cctaccataa acccgaggat gatgccgaaa aattggacta
    11821 tcccggaatt gcagccatat caggttttgt agccgacgcc gctgtccagc tgtcgaacgc
    11881 agaaacgctt gaaccatcac gcaggtttgt agcccagcaa aataccaagc tgcccaagtt
    11941 tggcctgcgg cttggtttcg gtacctccgg tcagaactac agcaacgaat tctacatggc
    12001 aaaaaacatt tttgcctttc aagccggatt ttacgccaag caaaggctca atcgccgttt
    12061 tgcgctccaa cccgaaatta tctaccaaac aatgggcagc aagcataccg atggaaccct
    12121 acgtacccac gaggttagca ttcccgttaa catcagatta agcttaagcc ctgcaagttc
    12181 agccagcacc tttatctcaa atgatttcta cattttcacc gggccttact actcctaccg
    12241 gtttggaggc ggcctgggag gaaaggatat ggatttcgac aatacctttc gcagggagga
    12301 ctatggaatt cagggtggtt tcgggtttga aataatgaac tttcagcttc aaatttcacg
    12361 gttccgctcg ttcaggtcaa tcgacagaac aaccaccatc atgccctacg gcatgatgtg
    12421 gacgttggga ctgcggtttt gaaaaaccta cccacacagg cc
//
LOCUS       DAOSHM010000065        12132 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_4425_length_12132_cov_20.725263, whole genome shotgun
            sequence.
ACCESSION   DAOSHM010000065 DAOSHM010000000
VERSION     DAOSHM010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 12132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12132
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4425_length_12132_cov_20.725263"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..1245)
                     /locus_tag="PK839_08915"
     CDS             complement(4..1245)
                     /locus_tag="PK839_08915"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG72894.1"
                     /translation="MRTENYIARRFFKGTNGMSLSRPFIRITITSVALSIAVMIVAVA
                     VITGFKQEIKAKVAGFGSHIQITNYDSNQSLETLPLPSNLKCVEDLKAIKGVRHIQVF
                     GIKAGIIKTPSDMQGVALKGVSSDFDWSFFRQNLVEGSIISVSDSTPTNQTLISKTLA
                     NLLRLKVGDSYDVFFVQEPPRFRRFVVEGIYDTKMVEFDKLFVICDIGHIRRLNGWSS
                     NQVSGYEVLVDNIEKVDDIAIRVDDAVGYDFLDDGSRLKVQSLTERYSSIFDWLELQN
                     INAAVLIILMLLVAGINMISGLLIIILDKTGTIGLLKALGTNNESIRKIFLLQSAYIM
                     IRGLAIGTIAGIAICLLQKYLGIVKLDEEYYFLSVVPINMVWWHIVLLNLASFIVGLA
                     MMVIPSMVVSRISPDKTLKFD"
     gene            complement(1288..1920)
                     /locus_tag="PK839_08920"
     CDS             complement(1288..1920)
                     /locus_tag="PK839_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H family protein"
                     /protein_id="HOG72895.1"
                     /translation="MKSKNSYYVVWAGYNPGIYGSWEECQQQIKGFPNAKYKGFPTKA
                     GAEKAFSISYEKYMAMNPAQEAIFQIDENVPSPVYPSLAVDAAWNTATLEMEYRGVDA
                     KTGKEIFHQGPFPDGTNNVGEFLAIVHALALLNQKGSTIPIYTDSMNAIAWVRAKTAR
                     TKLEKTPRNQILFDLIQRAEKWLRTNQWSNPLLKWETHVWGEIPADFGRK"
     gene            complement(2358..3533)
                     /locus_tag="PK839_08925"
     CDS             complement(2358..3533)
                     /locus_tag="PK839_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010801978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HOG72896.1"
                     /translation="MKFFPTILLIFNFLAATSQDKTTVEAGSEQVPEYLPEILGRNVA
                     VVANHTSLVKGVHLVDTLLSSGVKVRRIFSPEHGFRGNFEAGIPVASSTDYQTGLPVV
                     SLYGGSKQPTKEQLRDIEVVLFDLQDVGVRFYTYLSTLHYMMEACAINNVRLIVLDRP
                     NPNGFYVDGPVLDTANRSFVGLHPVPLVYGMTVGEFALMINGEGWLSKSRKCNLSVIP
                     CKGYSHRITMPLPVKPSPNLPNHLAVLLYPSLGLFEGTAVSVGRGTDYPFQMFGYPAF
                     PLRNFWFLPVEKKGASTNPPYKGKKCYGIDLRDYPVEYFLGKKSINIEWLVYAYNTYP
                     TKSKFFNSFFRLLAGTDELQRQIEGGMSADEIRRSWSDDLAKYRLIRKKYLLYSDFD"
     gene            complement(3761..4960)
                     /locus_tag="PK839_08930"
     CDS             complement(3761..4960)
                     /locus_tag="PK839_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOG72897.1"
                     /translation="MPKISEKGLAMPASPIRKLVPFAEEAKRKGRKVYHLNIGQPDIP
                     TPQVALEAIRHHDLKVVEYSHSAGNESYRKGLASYYKGIGVDITHNDIIVTTGGSEAI
                     TIALMSCLNPGDEVIIPEPFYANYNSFALQAGVVVKPIVSSIQNDFALPPISEFEKLI
                     TPKTKGIIICNPNNPTGYLYSQQEIEQLATIVKKHDLYLFSDEVYREFCYDGNTHFSV
                     MRLTGLDNNIVLIDSVSKRYSMCGVRIGALISRNKEVIATAMKFAQARLSPPSFGQIA
                     AEAALNTPPEYFKEVYDEYINRRNFMVDALNKMEGVYCPKPKGAFYSVVKLPIDDSDK
                     FAQWLLESFEYKNQTVMVAPATGFYSTKGLGKNEVRIAYVLNTEDLRNAMECLEVALK
                     QYPGRKL"
     gene            complement(4986..5402)
                     /locus_tag="PK839_08935"
     CDS             complement(4986..5402)
                     /locus_tag="PK839_08935"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HOG72898.1"
                     /translation="MKRIFFAILLAAACSTMVNAQSTEKVDENAPEITFTSTEYDYGV
                     VAVNSDGTRKFEFTNTGKQPLILSNVSASCGCTVVEWAKDPVKPGEKGTITVKYNTAI
                     VGTFQKSIRVFSNAKTSPVMLVIKGEVKPNEPAPHQ"
     gene            complement(5989..8586)
                     /gene="mutS"
                     /locus_tag="PK839_08940"
     CDS             complement(5989..8586)
                     /gene="mutS"
                     /locus_tag="PK839_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOG72899.1"
                     /translation="MVETPLMKQYLSIKQKHPDAVLLFRVGDFYETFGEDAILASGVL
                     GITLTRRANGAASFVELAGFPHHALDTYLPKLVRAGYKVAICDQLEDPKLAKTIVKRG
                     ITELVTPGVAYNETILEHKENNFLGCIYLDKKLAGISFLDISTGEFLTSEGNMDYIDK
                     LLSNFKPKEVLVERSKLREFTEHFGDKYYTTRLDEWAFSEDAAREKLLNHFKTTSLKG
                     FGIDSLKLGVISAGAILYYLDITQHSQAEHISSISRIDEENYVWVDRFTSRNLELFTS
                     INEGAKTFIEVIDKTASPMGGRLLKRWVSLPLKNLNAINDRLNMVDYFFNNPDLRSTL
                     LDRIRGMGDTERIISKAAVGRITPREIVQLKNSLNLLADVKKACDESGEPTLLRIGEQ
                     LNLCTRIRERIEREIVADPPPLLGKGAVIANGVNSELDELRSILYSGKDYLLNLQQRE
                     AERTGIPSLKVSFNNVFGYYIEVRNTHKEKVPADWIRKQTLVSAERYITQELKEYEEK
                     ILGAEEKIQSLEQSIFLNLIHNLGEYIPSIQLNSNLVAQLDCLLSFSSCAHEYGYTKP
                     TVNQSDAISITQGRHPVIERMLPAGEEYVANDIYLDTTEQQIIIVTGPNMAGKSALLR
                     QTALIVLMAQIGCFVPAQAASIGLVDKIFTRVGASDNLSLGESTFMVEMQESASILNN
                     LSSRSLVLLDEIGRGTSTYDGISIAWAIVEYLHEHPNARAKTLFATHYHELNEMESTF
                     PRVKNYNVSIKELNDRVIFLRKLVRGGSEHSFGIHVARMAGMPPSVIKRAYEILAELE
                     KSQQNETLHKPVAEIGTRREGYQLSFFQLEDPVLKQIRDQIKTLDINNLTPLEALNKL
                     NEIKRLTGL"
     gene            complement(8743..10206)
                     /locus_tag="PK839_08945"
     CDS             complement(8743..10206)
                     /locus_tag="PK839_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008658217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HOG72900.1"
                     /translation="MSSTAIIILIVCYFTALFIISYFTSRRSDNQSFFIGNRRSPWYL
                     VAIAMIGTSISGVTFISVPGWVGTSQFSYLQMVLGYLLGYFVIANVLLPLYYRLQLTS
                     IYTYLDKRFGFYSYKTGAVLFLVSRIIGSAFRLFIVSSVLQLTVFDAWGIPFWVTVTA
                     TILLIWLYTRKGGVKTVLWTDTFQTITMLVAVILTIFFIAKELHLNFSGIVSTISHSE
                     LSRIWFFDDINNRFHFLKQFFGGAFITIVMTGLDQDQMQKNLSCRSYSDAKKNMYWYG
                     FAFLPVNLLFLSLGVLLYKFAEVKGISIPAQTDNLFPIIATQGYISPVVGILFVLGLI
                     SAAYSSADSALTALTTSFTIDILNTKDMDEATLQKVRKRVHLMISAVVVAVILIFNAF
                     NDRSVIDSIFTVAGYTYGPLLGLYAFGMFTRFQTNDKLVPLIALLSPIICLLLSTYSK
                     ELFGGYEFGFELVILNGLLTFTGLYFTRRKGVKIPKV"
     gene            10615..11241
                     /gene="recR"
                     /locus_tag="PK839_08950"
     CDS             10615..11241
                     /gene="recR"
                     /locus_tag="PK839_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013265871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HOG72901.1"
                     /translation="MNVQSYPSKLLETAVEEFSKFPGIGRRSALRLVLHLLRQDIEDV
                     DSFTRAITDLGHNVKYCRVCNNISDTDTCGICSSPHRDAGLICVVESIKEVMMIESTH
                     QFNGLYHVLGGVISPMEGIGPADLSIEKLVDRVNQGGVREVIMALSATTEGDTTNFYI
                     FKKLKDKPVLFSTLARGVAFGDELEFTDEVTLGRAILNRVKFDASIPD"
     gene            11336..12064
                     /locus_tag="PK839_08955"
     CDS             11336..12064
                     /locus_tag="PK839_08955"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG72902.1"
                     /translation="MGNRFTRILLLLLISSHSIAQTSFNELVSSANLNLKEKNYKAAA
                     DQFKKALEIQPDDTVALQGVIKCYTLSEDYKNAQRYADIALGKFPTYPEFHLLQGIIY
                     NLKGNHEEAISAFSHALELKPSNRTSVQILMNRASAEIRLDNFTDALADYNAAMELDP
                     RNTSIYTYRGLVNFRLGFYIDAVNDYTNAIDLEPNSALTYYNRGMTYLKLSEKQKACA
                     DFHMACKLGDMNACKMILTECGGR"
BASE COUNT         3085 a         2723 c         2978 g         3346 t
ORIGIN      
        1 atgctagtcg aacttaagcg ttttgtcggg gcttatacgc gaaaccacca tggagggtat
       61 aaccatcatg gcaagcccaa caataaatga agccagattg agtaaaacga tgtgccacca
      121 caccatgttg atgggcacca ccgacaggaa gtagtactcc tcgtcgagct taacaattcc
      181 taaatacttt tgtaacaaac aaatggcaat tccggcaatt gtgcctatag ccaaaccgcg
      241 aatcatgatg taggccgact gtaggaggaa aattttgcga atgctctcgt tgtttgttcc
      301 gagcgctttt agcaggccga tagttcccgt tttgtcgagg atgatgatga gaagacccga
      361 aatcatgttg atgccggcaa ccagaagcat gagtatgatg agcactgctg catttatgtt
      421 ttgcagttcc agccagtcaa atatgctgga gtagcgttcg gtcaggcttt gtacctttaa
      481 tcgtgaccca tcgtcgagaa aatcgtatcc cacggcatcg tcaacccgga tggctatgtc
      541 atccaccttt tcaatgttgt caaccagcac ctcgtaaccg cttacctggt tgctgctcca
      601 tccgttaaga cggcggatat gcccaatgtc gcagatgaca aatagcttgt cgaactccac
      661 cattttggta tcgtagattc cctctactac gaaacgtcgg aagcgtgggg gttcctgaac
      721 aaagaaaacg tcgtaggagt cgccaacctt tagccttaat aggttggcca gtgttttgga
      781 tatgagggtt tggttggtgg gggtggagtc gctaacgcta ataatgctgc cctccaccag
      841 gttttgacgg aaaaacgacc agtcaaaatc ggaggaaact cctttaagcg ccactccctg
      901 catatcggag ggtgttttaa taataccggc cttaatacca aaaacctgga tatgcctaac
      961 ccccttaatc gctttaaggt cctccacgca ctttagattc gatggtaacg ggagggtctc
     1021 aagcgactgg ttgctgtcgt agttggttat ctggatatgg ctaccaaatc cggctacttt
     1081 ggcctttatc tcctgcttga acccggttat tacggctact gctactatca tcactgcaat
     1141 gctaagggca acagaggtaa tggttatacg gataaaaggc ctggacaggc tcatgccgtt
     1201 tgtgccttta aagaatcgtc gggctatgta gttttcggtg cgcacaggtt gaaatttctt
     1261 tatggaataa gttgggtagg atttgtgcta ttttctgccg aaatcggcag gtatctcacc
     1321 ccacacatgg gtttcccatt tcagcagggg gttgctccac tggtttgttc ttagccactt
     1381 ttcggccctt tgtatcaagt cgaatagtat ctggttacgg ggagttttct ccagctttgt
     1441 tctggccgtc ttagcccgta cccaggcaat ggcgttcatg gaatcggtgt agattgggat
     1501 ggtgctgcct ttctggttca acagggccag cgcatggaca attgccagga actcccccac
     1561 gttgtttgtc ccgtccggga agggtccctg atggaatatt tcctttccgg ttttggcatc
     1621 cacgccccta tactccattt caagggttgc ggtattccat gccgcatcaa ccgccagcga
     1681 agggtaaaca gggcttggta cgttttcatc aatttggaat atggcctcct gtgccggatt
     1741 cattgccatg tacttctcgt agcttatgct aaaagccttc tctgcaccag ctttagtagg
     1801 aaaaccttta tattttgcat tcgggaaccc tttaatttgt tgctggcact cctcccagct
     1861 cccgtaaatg cccgggttgt aaccagccca caccacgtag tagctatttt tgcttttcat
     1921 agtgcataat ttttaggtta agattacaaa tacttgccca tacctttgtg ccaagcggcc
     1981 gagcggatgg aacacccata ttttggcctc gccgaatccg caggtaactg ccgcttgctc
     2041 atgcacgttt gtgcaagctg cattgcattg gtgtttcatt ggttttttag cttgatgctt
     2101 atgccatgaa acaaaccatt gggaaacaac atgataattt gaaaagtggc ttttatcaag
     2161 gttgacttat ttctttcggt tttacctttc aaaggtacat gatgatttct gattaatacg
     2221 ctgatgaata aaaactttta atgtatcacg gatatgaggg atgatacaga ataggacaga
     2281 gtttatcagt aaaattcggc aatatctata taagcagtat gctatattcc catattttaa
     2341 aagtttgatt gaaaaagcta gtcaaaatcg gagtagagca agtatttttt tctgatcaac
     2401 ctgtacttag ccaggtcgtc cgaccatgac ctgcggattt cgtcggcgct cataccgcct
     2461 tcaatctgcc tttgcagctc gtcggtgccg gcaagcagcc tgaagaagct gttaaaaaac
     2521 ttcgacttgg ttggataggt attgtaggcg taaacgagcc actcgatatt tattgatttt
     2581 ttaccaagaa agtactccac aggatagtct ctgaggtcga tgccgtaaca ctttttaccc
     2641 ttgtagggtg gattagtgct ggcccccttt ttctcaaccg gtaaaaacca gaagttcctg
     2701 agggggaaag ccgggtatcc aaacatttga aatgggtaat cggttcccct gcccacgctg
     2761 acggcagttc cttcaaaaag acctagcgat gggtatagca gcaccgccaa gtggttgggt
     2821 aaatttggtg atggttttac cggaaggggc atggttattc ggtggctgta tcctttgcag
     2881 gggataacac tcaggttgca tttacgactc ttggaaagcc atccctcgcc gtttatcatc
     2941 agggcaaact caccaacagt cattccataa accagcggaa caggatgaag ccctacaaac
     3001 gaacggttgg cggtatcaag cacagggcca tcaacataaa atccattggg attaggccgg
     3061 tcaagcacta ttaacctgac gttgttgatg gcacatgctt ccatcatgta gtgtagggtt
     3121 gataggtagg tgtagaacct cacgccaaca tcctgcaggt cgaagagcac cacctcaata
     3181 tcgcgaagtt gctccttggt gggctgcttg ctgccaccat agagcgaaac aacaggcagt
     3241 cctgtttggt agtcggtact gcttgcaaca ggtattcccg cttcaaagtt accacggaaa
     3301 ccgtgctccg ggctgaaaat tcgcctcacc tttacacctg atgacagcag ggtgtcaacc
     3361 aggtgcactc ctttcaccag cgaggtgtgg ttggccacca cagcaacgtt tcgccccaag
     3421 atttcgggaa ggtactccgg cacctgctcc gatccggctt caacggtagt tttgtcctgt
     3481 gaagttgccg ccaaaaagtt gaaaataagc aatatggttg gaaaaaactt cataggatgg
     3541 cgagttgttg ctgattctga tagtttaaat agtccggaat gcttagggta gcgtagatat
     3601 gcatttggat aggatacctt aaatgccaag tttaaaaccc ttgtgctaaa gtagaaatat
     3661 tttttgattc cgcctagtga agctgaagta catggtttat aaagcacaaa aagggtggtt
     3721 ccgcaggcca ccctttattt tgcacttatt tcaagttctg ctaaagctta cgaccgggat
     3781 actgcttaag ggctacctcg agacattcca tggcatttct caaatcctcg gtgttcagca
     3841 cgtaggcaat acgcacctcg tttttaccca atccttttgt ggagtaaaaa ccggttgcag
     3901 gagctaccat gacggtttgg ttcttatact caaaactttc gaggagccac tgggcaaact
     3961 tatcgctgtc gtcaattggc agcttcacaa cgctgtagaa ggctcctttt ggtttggggc
     4021 agtaaacccc ctccatcttg ttaagggcat ccaccatgaa gttacgacgg ttgatatact
     4081 cgtcatatac ctccttaaaa tactctggtg gcgtgttgag ggcagcttcg gcagcaattt
     4141 gcccgaagga tggcggtgaa agccgtgcct gtgcaaattt catggcggtt gcaatcactt
     4201 ccttgttacg tgaaattaag gcaccaatcc gaacgccaca catgctgtag cgtttggaaa
     4261 cggaatcaat taggacaatg ttgttatcaa gcccggtgag cctcatcacc gaaaaatggg
     4321 tattcccgtc gtagcaaaat tcacggtaca cctcatcgga gaataggtag aggtcgtgct
     4381 tcttaacaat ggttgccagc tgctcaattt cctgttggga gtaaaggtat ccggtggggt
     4441 tgttggggtt gcagataata atgcctttgg ttttaggtgt gatgagcttt tcaaactccg
     4501 aaattggggg aagcgcaaag tcgttttgta tggacgaaac aatgggttta accacaactc
     4561 ccgcctgtag ggcaaagctg ttgtagttgg cgtagaatgg ctcggggata atcacctcgt
     4621 cacccgggtt caggcaggac atcagcgcaa tggtaatggc ttctgatccc cctgttgtaa
     4681 cgatgatgtc gttgtgggtg atgtcaacgc caatcccctt atagtagctg gcaagcccct
     4741 tgcggtagct ttcgtttcct gccgagtggc tatactcaac cacttttagg tcgtgatgtc
     4801 gaatagcctc aagcgctacc tgaggagtag gaatatcggg ctgtccaata ttcaggtggt
     4861 aaaccttacg cccctttcgt tttgcctctt cggcaaaagg aaccagcttt cggattggag
     4921 aagctggcat agccagcccc ttttcggata tttttggcat gtctttatgt gatttggttt
     4981 gtaatctatt gatgtggtgc aggctcgttt ggtttaacct cacccttaat tactagcata
     5041 accggactgg ttttggcgtt ggagaaaacg cgtatgcttt tctggaatgt tccaactatg
     5101 gctgtgttgt acttaacggt aatggttccc ttttcgcctg gcttaacagg atccttggcc
     5161 cactccacaa ccgtgcagcc gcatgatgcc gaaacattac ttaaaatcaa gggttgtttc
     5221 ccggtgttcg taaactcgaa tttacgggtt ccatctgaat ttacggcaac taccccgtag
     5281 tcgtactccg ttgatgtaaa ggtaatttca ggagcatttt cgtccacctt ttcggtgctt
     5341 tgtgcattaa ccattgtgct gcaagctgcg gccaacaaaa tggcgaaaaa aattcttttc
     5401 atcgtaatct cgttttagca aaaagttaaa caatatattg taaggcacac tttttatcag
     5461 aggagcttta ggctgagttg aaattgcacg aagttggcag tgcatttcag gttgcaaagt
     5521 tagggttctt ttagctattt ggtttaaact actgttaata aatgtaattg tacaatccca
     5581 cagctgggcc aaggctacaa cttgataaag aagtgcactg ggttgggcaa agtttagcaa
     5641 agcgttcatc cgtcgagcta ttcttttagg ctgcgaccat ttttttagtt gttttatgtt
     5701 tggctgaggc tataaaaagt aataaacaat tcgtagtctg cggttggttg caccgccgtt
     5761 gggctatcga aagcgggatc agcctgctat tcttgaaatg acatacggtg gaaaacttac
     5821 aattagctat atttacaggg cagagcccga agagatagat acgaccgagg cacacagcct
     5881 aatccaaaca gggaatctgc tagatctact tttctgaaat caagggtagg tttctttggt
     5941 ttttatggga tgctcggatg ttagcagatg tagaatagca atcggtggct agaggccggt
     6001 aagccgctta atttcattca gcttgtttag cgcctccagc ggggtaaggt tgttgatgtc
     6061 cagcgtttta atctggtcgc gaatctgctt cagcacgggg tcctcgagct ggaagaagct
     6121 cagctggtaa ccctccctcc gggttccaat ttctgctacc ggtttatgta gagtttcatt
     6181 ctgttgcgat ttttcaagtt ctgcaagtat ttcgtaggcc cttttaatca cgctgggtgg
     6241 catgccggcc atacgcgcaa cgtggatgcc aaaactgtgc tcgcttccgc cgcgaacgag
     6301 cttgcgcaaa aagattaccc ggtcgttgag ttcctttatg gagacgttgt agttcttcac
     6361 gcgggggaag gtggactcca tttcgttcag ctcgtggtag tgggtggcaa aaagggtttt
     6421 agctctggcg ttgggatgct catgcaggta ttccacaata gcccatgcaa tggaaatgcc
     6481 atcgtatgtt gaggttcccc gacctatttc gtcaagaagc acaaggctac gggacgagag
     6541 gttgttgaga atactggcgc tttcctgcat ttcgaccata aatgttgact caccgagcga
     6601 gaggttgtcg gaggcaccaa cccgggtaaa aattttatca accagcccaa tgcttgcagc
     6661 ttgcgccgga acaaaacagc ctatctgggc catgagaaca atcagggcgg tttgcctgag
     6721 caaggctgat tttcctgcca tatttggtcc ggtaacaata ataatctgtt gctcggtagt
     6781 gtcgaggtag atgtcgttgg ccacgtactc ctcgcctgcc ggtagcatgc gttcaatcac
     6841 cggatgcctg ccctgggtta ttgagatggc gtcgctctgg ttaactgttg gtttagtgta
     6901 tccgtactca tgggcgcagc ttgaaaacga cagtaagcaa tcgagctggg caacaaggtt
     6961 ggagtttagc tgaatggagg ggatgtactc gcccaagttg tggataagat tcaggaaaat
     7021 tgactgctca agggattgga ttttctcctc tgcacccaga attttctcct cgtattcctt
     7081 aagctcctgg gtaatgtagc gctctgccga aacaagggtt tgcttacgta tccaatcggc
     7141 tggaaccttc tccttgtggg tattcctaac ctcgatgtag tagccaaata cgttgttaaa
     7201 gctaactttt agcgagggta tgccagttcg ttcagcctct cgttgctgta aattcaacaa
     7261 gtaatccttc ccggagtaaa ggatgctacg gagttcatcc agctcgctgt taacgccgtt
     7321 ggcaataaca gctcccttgc ccagaagcgg gggtggatcg gcaacaatct ccctttcaat
     7381 cctttcgcga attcgggtgc aaaggtttag ctgctcccca atgcgcaaaa gggtgggctc
     7441 gcccgattca tcgcaggcct tctttacgtc tgcaagtagg ttgagcgaat ttttcagctg
     7501 aactatctct cggggggtaa tacgaccaac ggcggctttt gatataattc gctcggtgtc
     7561 gcccataccc cttattctat ccagcagtgt acttcttaaa tcggggttat tgaagaagta
     7621 atccaccatg ttgagcctgt cattaatggc attcaaattc ttcaagggta gcgaaaccca
     7681 tcgttttaat aacctaccac ccattggcga tgcggtttta tcaataacct cgatgaatgt
     7741 tttagccccc tcgttaatgg aggtgaacag ctccagattt cttgaagtaa agcggtcaac
     7801 ccaaacgtag ttttcctcat caattctcga aatgcttgaa atatgctcag cttggctgtg
     7861 ttgcgtgatg tcaaggtagt agagtatggc tcccgccgag attactccca gttttaagct
     7921 atcaattccg aatcccttga gcgaggttgt tttaaagtgg ttaaggagtt tttcgcgagc
     7981 ggcatcctcg ctaaacgccc attcatcgag ccgggtggtg taatacttat cgccgaaatg
     8041 ttcggtaaac tcgcgcagtt tcgagcgttc aaccagcacc tctttgggtt taaagttgct
     8101 tagcagctta tcaatgtaat ccatgttgcc ctcggaggtt aggaattcac ctgttgatat
     8161 gtcgaggaac gagatgccag ccaacttctt atccaggtaa atgcaaccca gaaagttgtt
     8221 ttccttatgc tcaagtatgg tttcgttgta tgctacaccg ggcgttacca gctcggtgat
     8281 gccccttttc acaatggttt tggccagttt tgggtcttcc agctggtcgc aaatggcaac
     8341 cttatagcct gccctaacga gttttgggag gtaggtatcg agggcatggt gcggaaatcc
     8401 tgccagctcc acaaaagaag ctgctccgtt ggcccttcgg gtgagggtta taccgagaac
     8461 gcctgatgcc aggatggcat cctctccaaa ggtctcgtag aaatcgccca ccctaaagag
     8521 tagcactgca tcgggatgtt tctgcttaat gctaaggtac tgcttcatca ggggtgtttc
     8581 caccatgccc ttattcctgc cttcgtccaa ggtgtttgct tttgtttacc cacaaaatta
     8641 tcaaatcctc accaagtttc agcttaaaat gtaacatttt ggtaaaccgg attagcagtt
     8701 ttagcctgat taaatgagta ggggcaatgg gatgaaaaag ggctatacct tcggaatttt
     8761 tacacctttt cggcgtgtga agtaaagccc cgtaaaggtt aggaggccgt tcaggattac
     8821 cagctcaaat ccgaactcgt acccgccaaa aagttccttg gagtatgtgc ttagcaataa
     8881 acagatgatg ggcgacagca gggctattag cggaaccagc ttatcgtttg tttgaaaacg
     8941 ggtgaacatg ccaaaagcgt atagtccaag aagggggccg taggtgtaac cagctacggt
     9001 aaaaatggag tcaattacac ttcggtcatt aaatgcgttg aagataagaa ttacggctac
     9061 aacaacagca gatatcatta aatgaacccg tttccgcacc ttttgaagtg tagcctcatc
     9121 catgtccttt gtgttgagga tgtctatagt gaacgaggtg gtaagagcag taagggctga
     9181 gtcggcgctg gagtaggcag cagaaatcaa ccccagcaca aagagaattc caacaaccgg
     9241 tgaaatgtag ccttgcgtgg ctataatggg gaataggttg tcggtttggg cgggtatgct
     9301 tattcccttt acctctgcaa atttgtatag cagaactccc agcgaaagga aaaggagatt
     9361 aacggggagg aaggcaaacc cgtaccagta catgtttttc ttggcgtcgg agtagcttcg
     9421 gcagctcagg tttttctgca tttggtcctg gtcaaggccg gtcataacta ttgtgatgaa
     9481 cgctcctcca aaaaactgct taaggaagtg aaaccggttg ttgatatcgt cgaaaaacca
     9541 gattctggat agctcgctat ggcttatggt gcttactatc cctgaaaaat tcaggtgtag
     9601 ctctttggca atgaaaaaga tagtaaggat aaccgctaca agcatggtaa tagtttggaa
     9661 ggtatcggtc cagagcacgg ttttcacccc tcctttacgg gtgtagagcc agattaaaag
     9721 aatggtggcg gttacggtta cccaaaaggg aatgccccac gcgtcgaaaa ccgtaagttg
     9781 gagcacgctc gaaacgatga acagccggaa cgccgagcca attatcctgg ataccaggaa
     9841 aagaaccgct ccggttttgt aggagtaaaa cccaaagcgt ttgtcgaggt aggtgtatat
     9901 tgatgttagc tgaagccggt agtataatgg cagcagcacg ttggcaataa caaagtagcc
     9961 gaggaggtag cccagcacca tttgcaggta ggagaactgg ctggttccaa cccatccggg
    10021 aaccgaaata aaggtaactc ccgaaatgga tgttcctatc atggcgatag ccaccaggta
    10081 ccaaggcgag cgcctgttac caataaaaaa cgactggtta tcggaacgcc tggaggtgaa
    10141 gtaggaaata ataaagagtg ctgtgaagta gcagacaatc agaatgataa ttgcagttga
    10201 cgacataaac tcctggtttt aggcgctgca agtttaatca atttaagcca aaatatgcta
    10261 gggttcggga cagcggtttt tgattaggtg aatggggatt cgttttatac tgctcatccg
    10321 ccaagcataa attccaaaat tttgttttat gcttgggtaa ttctttcagg taatctcctg
    10381 cttgaaggga taaagctggt atttcatctt attttccttt aatacgggga ggaatttgcc
    10441 atttaattct aaattcatgt agatttgtgg aaactttaaa ttttatcgaa cccaactttc
    10501 cgattgtgag tggctgtgtc ccaagttgaa catgggaata gcctaaggga gtacgaagtg
    10561 ccaaaaaaat tgatggttgc aaggctgttg ataaaaatga ataaagaata gagtatgaat
    10621 gtccagtcat atccctcaaa gttgctggaa actgctgttg aagaattttc aaagttcccc
    10681 ggaataggca ggcgttcggc cctgaggttg gtgctccatc tgctacgtca ggacatagag
    10741 gatgttgata gcttcaccag ggccataacc gatctgggtc ataatgtgaa gtactgcagg
    10801 gtgtgcaaca atatatccga tacggatact tgcggcattt gctcatcgcc ccatcgcgat
    10861 gcggggctta tttgcgtggt ggagagcatt aaggaggtca tgatgattga gagcacccac
    10921 cagttcaacg ggttatacca tgttcttgga ggcgtgataa gccccatgga ggggattggc
    10981 cctgccgacc tgagcatcga aaagctggtt gaccgggtaa accagggagg agtaagagag
    11041 gttataatgg ctcttagcgc taccaccgag ggcgatacca ctaattttta catctttaaa
    11101 aagttgaagg acaagcctgt tttattctcc acgttggcac gaggggtggc ttttggtgac
    11161 gagctggagt ttaccgacga ggtaaccctg ggcagggcaa tcctcaacag ggtgaagttc
    11221 gatgcttcta tcccggatta gctagaggaa aaatttgttt tggcatcacg aattttggtg
    11281 taactttatg attggtaatt tggtaaaaca tatcatccag caaatccctt tagctatggg
    11341 aaatcgattt acacgcattc tcctgctact ccttatttct agccacagta ttgcccaaac
    11401 cagcttcaac gagctggtaa gctcggcaaa cctgaacttg aaggaaaaaa actataaggc
    11461 tgccgccgat caattcaaaa aggcccttga gattcaacct gacgataccg ttgccctaca
    11521 gggggtaatc aaatgctaca ctctttcgga agattacaag aatgcccagc ggtatgccga
    11581 tattgcactt ggcaagttcc ctacctatcc cgagtttcac ctgttgcagg gaattatata
    11641 caacctcaag ggcaaccatg aggaggctat ttctgccttt tcccacgcgc tggagcttaa
    11701 accctccaac cgaacgtccg tacagatcct gatgaacagg gcttctgctg aaatcaggct
    11761 ggacaatttt accgatgccc ttgccgacta caacgccgcc atggagctcg atccccgcaa
    11821 taccagcatc tatacctacc gggggttggt taactttagg cttggcttct acattgatgc
    11881 cgttaacgac tacaccaacg cgattgatct tgaaccaaac tctgccctta cctactacaa
    11941 caggggtatg acctacctga aactttccga aaagcagaaa gcctgcgccg atttccacat
    12001 ggcctgcaag ctgggcgaca tgaatgcctg caagatgatt ttaaccgagt gcgggggtag
    12061 gtagggctat ttcggaaaaa actagtttta catgtaattc atcttcccca aagctgaatg
    12121 ttcaactctt tt
//
LOCUS       DAOSHM010000066        12107 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_4440_length_12107_cov_20.000913, whole genome shotgun
            sequence.
ACCESSION   DAOSHM010000066 DAOSHM010000000
VERSION     DAOSHM010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 12107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12107
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4440_length_12107_cov_20.000913"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..881)
                     /gene="folD"
                     /locus_tag="PK839_08960"
     CDS             complement(<1..881)
                     /gene="folD"
                     /locus_tag="PK839_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HOG72903.1"
                     /translation="MELLDGKKISEEIRAEIASEVEKIKSQGGKIPYLVAMLVGHDGA
                     SETYVHNKELACAQVGFRSQVLRFEETISESGLLAEIQKLNANPEVDGFIVQMPLPKH
                     IDEQKIIEAIDPKKDVDGFHPVNVGRMVIGLPSYISATPDGIVELLRRYKVETAGKHC
                     VVLGRSNIVGRPVANLLLHKGYPGDCTVTICHSRTPNLKELTLQADILIVAMGKPEFV
                     TADMVKNGTVVVDVGITRIPSEKTKSGWKLVGDVKFDEVAPKCSFITPVPGGVGPLTI
                     ASLLKNTLKAARKEVYG"
     gene            complement(1017..2351)
                     /gene="ffh"
                     /locus_tag="PK839_08965"
     CDS             complement(1017..2351)
                     /gene="ffh"
                     /locus_tag="PK839_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018110035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HOG72904.1"
                     /translation="MFENLTEKLERSFKILKGEGKITEINVAETLKEVRKALLDADVN
                     YKVAKSFTDEVKRKALGMNVLNAIRPGQLMVKVVHDELAELMGGKAANINLNGNPAII
                     LIAGLQGSGKTTFSAKLASYLKTKKGKSPLLVAGDVYRPAAIEQLKVLGDQVGVPIFT
                     VPGSSNPVDIAKLSIKQAKTLGRDTIIIDTAGRLAVDEEMMNEVAAIKKVVSPQEILF
                     VVDSMTGQDAVNTAKEFNDRLNFDGVVLTKLDGDTRGGAALSIRAVVDKPIKFISTGE
                     KLDALDVFHPERMADRILGMGDIVSLVEKAQEQFDADKARELNKKILKNRFNFNDFLE
                     QIGQIKKMGNLKDLAGMIPGVGKAIKDVDLNDDAFKSTEAIIKSMTPAEREEPSLING
                     SRRKRIADGSGTSIVEVNRLIKQFEDMRKMMKMASGGGAKNLARMMSQQPRR"
     gene            complement(2470..4266)
                     /gene="argS"
                     /locus_tag="PK839_08970"
     CDS             complement(2470..4266)
                     /gene="argS"
                     /locus_tag="PK839_08970"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015547745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOG72905.1"
                     /translation="MSTEQYIQDIVAETVLKLYGVKPEVNQLQIQKTRKEFEGDYTLV
                     AFPLLKLTRKSPEETCRQIGEMLLQSYPEVSAINVIKGFLNITLSKTYWSGVINEIAS
                     TEDYGFAPTPTENANPTIVEFSSPNTNKPLHLGHIRNNLLGFSISRILEKSGKRVIKV
                     NLVNDRGIHICKSMVAWLKYGNGETPQTSGKKGDHLVGDYYVRFEKEYKLQVEQLTSS
                     GMDKDEALTKAPIIIEAQEMLRKWEAKDAETIRLWKMMNGWVYEGFEKTYQNLGITFD
                     KIYYESQTYLVGKKVVNEGLDRGIFFRKSDGSVWVDLTADGLDQKLLLRSDGTSVYIT
                     QDIGTAIERYNEFHFNEHIYVVGNEQDYHFQVLKLVMKKLGYTWYEALYHLSYGMVLL
                     PEGKMKSREGTVVDADDLISEMLQTAGDVSRELGKLDQMSDEEASETIRMVGLGALKY
                     FILKVDPKKNMTFNPGESIDFNGNTGPFIQYTHARIRSVLRKAGNVNDLLNQKVTPES
                     ISFQKELELIQLLHLFPDTIKIAAQTHNPAEIANYTYELAKQYNQFYQDLPILREPNV
                     EVKHFRLLLSQAVARVIKQAMWLLGIDVPEVM"
     gene            complement(4405..7686)
                     /gene="secA"
                     /locus_tag="PK839_08975"
     CDS             complement(4405..7686)
                     /gene="secA"
                     /locus_tag="PK839_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HOG72906.1"
                     /translation="MAGGSFLSKIFGTKADRDMRELMPMLNTIRETYKKYPSLSNDEL
                     RSESAKLKAKVHDYIAADEAEVERLKQMMESDEIGYDEKEKIYKDVEKLQQTIDDKIE
                     EILNIVLPDAFSIVKETARRFKENETIEVTATQFDKDLAAQKDFVTIQDDKAIYKNRW
                     MAGGNEIVWDMVHYDVQLIGGVVLHQGKIAEMATGEGKTLVATLPVFLNALAGKGVHV
                     VTVNDYLARRDSEWMGPIYEFHGFAVDCIDKHQPNSEARRKAYNADITFGTNNEFGFD
                     YLRDNMAIAPDDLVQRKHHFAIVDEVDSVLIDDARTPLIISGPVPKGDDQMFEEYKPK
                     VEKLVNAQRALITQLLADAKKLIAEGNTDEGGKLLLRAFKGMPKYKPLIKFLSEQGNK
                     ALLLKTENYYMQDNNRNMHLITDDLFFVIDEKLNSVELTDKGNDLITSSSEDSKFFIL
                     PDIGAEIAEIEKMPISHNEKLAQKDELLRDFSVKSERVHTVHQLLKAYTMFEKDVEYV
                     VMDNKVKIVDEQTGRILEGRRYSDGLHQAIEAKERVKVEAATQTFATITLQNYFRMYH
                     KLAGMTGTAETEAGEFWTIYKLDVVVIPTNQPVIRKDMEDLVYKTKREKYNAVIDEIV
                     KLTGENRPVLVGTTSVEVSELLSRMLKLKGIKHNVLNAKQHQREAEIVAQAGLPKTVT
                     IATNMAGRGTDIKLTPETRAAGGLAIIGTERHESRRVDRQLRGRSGRQGDPGTSQFYV
                     SLEDDLMRLFGSDRIARIMDRLGLKEGEVIQHPMISRSIERAQKKVEENNFGIRKRLL
                     EYDDVMNAQREVIYTRRRHALYGERLDIDISNMFWDVCEAMVSSFKGSESYEDFSLET
                     IKNFSLEAPVTEEEYENLKPDEIAERLYHQALESYDRKAASIAKQAYPVIKDVYEKQS
                     AIYENIVVPISDGQKTYQIVTNLKKAYESGGKELIRSFSKSIILYTIDEYWKEHLREM
                     DDLKQSVQNAAYEQKDPLLIYKLEAYELFRTMLENLNRDIVSILAKAFIPLRSANEVQ
                     EARQRSHTDMSRYQTSRTDNLQAGAPTQQPNREPVKVGIKVGRNDPCPCGSGKKYKNC
                     HGRDE"
     gene            complement(8101..9831)
                     /locus_tag="PK839_08980"
     CDS             complement(8101..9831)
                     /locus_tag="PK839_08980"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007217249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HOG72907.1"
                     /translation="MENFIVSARKYRPSTFKSVVGQTSITTTLKNAIQRKQLAQAYLF
                     CGPRGVGKTTCARIFAKTINCLNLTDENEACNECESCKSFSTNRSFNIHELDAASNNS
                     VEGIRKLIEQVRIPPQIGKYSVYIIDEVHMLSASAFNAFLKTLEEPPAHAIFIMATTE
                     KHKILPTILSRCQIFDFNRIRVEDTVKYLEYIAKTEGIDYEVEALNIIAQKADGAMRD
                     ALSIFDQVVSFSENHITYEKVIENLNVLDYQYYFRLTDNFLSGNMTDSLKIFDELLSK
                     GFDAQQFITGLSQHLRNLLVCKDSDTIQLLEVGAAIAEKYTKMASTCPIDFLVNALQL
                     SNQYDVSFRQSPNQRLHVELLLIRLCGLTQEKKKSESPANIEEGDVVEQKPQKQTKPA
                     QKVKHETYEPAAVNEPSTPIIPKTTSLKGALKKNTVESYTASDIDQPEIPSVAVNLPN
                     DSEYKAITQDMLGKAWSSFAESIKEEKPGQYSLLQLYGPELLSDGKAYIRFETQIQVD
                     MFREIKTDLLTFLKQSLKVNPPEILEEIAETDEKNKPRLFTADDKLKFLLQHNPALRK
                     MKQKLNLDLE"
     gene            complement(9958..11118)
                     /locus_tag="PK839_08985"
     CDS             complement(9958..11118)
                     /locus_tag="PK839_08985"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOG72908.1"
                     /translation="MKPFQMFVFITIALGVYLLVNLFIYYQTRVVFSIPRWGILLKII
                     FLALVLAYPVGRGLEAIFDGNTFTPLVRIGSMWLGAMLYLFMAFLVVWIVVVSTHTFG
                     SANPIHARQVGVALVYALVSILLIAGYTNAITPIVRKIEVRVDKPLRGGKLRVVAVSD
                     IHLGTVIGKRDLAKLVKMANAQNPDVVLLVGDIFDEDVTPVINGGMGTLLEQFKSKYG
                     VFAVTGNHEFYGGFEQKIKYLQQHGVKLLRDSTVLVDSSFYIIGRDDRQSSYMLKRER
                     KSVEELISEVDLRKLTILLDHQPYNLDDAARCGVDLQLSGHTHNGQLWPFNYITKAMY
                     EVSHGYYQKGGTHYYVSSGFGTWGPRLKIGNRSEVMVVEITGNPQNSTPEAS"
     gene            complement(11416..11826)
                     /gene="rnk"
                     /locus_tag="PK839_08990"
     CDS             complement(11416..11826)
                     /gene="rnk"
                     /locus_tag="PK839_08990"
                     /inference="COORDINATES: protein motif:HMM:NF004396.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HOG72909.1"
                     /translation="MSRITLNRLDYERIKRHLEVARQLKSITNTEADKLLGELESAQV
                     VNPESIPSDVVTMNSIVKISFLNTGKEIQFRIVYPHEANLKENKISIFSPIATALIGY
                     RVGDEIEWLVPAGPTKIRIEEIVYQPEASGNFTL"
BASE COUNT         2840 a         2836 c         2765 g         3666 t
ORIGIN      
        1 tatccgtaaa cctccttcct ggctgccttg agggtatttt tgagcaacga ggcaatggtg
       61 aggggaccaa caccaccggg taccggggta ataaagctgc atttaggcgc cacctcatcg
      121 aatttaacat cgcccaccag cttccatccc gatttggttt tttcgcttgg aattctggtt
      181 atccccacat caaccactac tgtaccattt ttaaccatgt cggctgtaac aaattcaggc
      241 tttcccatgg ctacaattag tatatcggcc tgcagggtaa gttcttttaa gttgggtgtt
      301 cggctgtggc agatagtaac cgtgcaatcg ccagggtagc ccttgtgaag cagcaggttg
      361 gctacagggc gacctacaat gttgctccta cccagcacca cacagtgttt tccggcagtt
      421 tcaaccttat accttcggag tagctccaca attccatcgg gtgttgccga aatgtaggaa
      481 ggaagcccaa taaccatccg acccacgttt accgggtgaa agccatcaac atcctttttg
      541 gggtcaatgg cctcaataat tttttgctca tcaatatgct tgggaagtgg catctgcact
      601 atgaagccat ccacctcggg gttggcattc agcttttgta tttcagccag tagcccgctc
      661 tcactaattg tctcctcaaa gcgtagcacc tgcgacctga atcctacctg ggcgcaggcc
      721 agctccttgt tgtgaacgta ggtctcgctt gccccatcat ggccaaccag catggctacc
      781 aggtagggga tttttccccc ttggcttttt atcttttcaa cctccgaggc gatttcggct
      841 cgaatttctt cagatatttt tttcccatct agtagttcca ttattcaaga ttcaagattc
      901 aagattcaag gtttaaggtt taaggtttaa ggcatccatc ttcctcccct taacttttca
      961 gggtttacga tttcgctttt atccttgtat tatttaatat ctcactctac ttttctctac
     1021 cttcttggct gttgtgacat catccgtgca agatttttcg caccacctcc ggatgccatc
     1081 ttcatcattt tacgcatatc ctcaaactgc ttaatcaacc ggtttacctc cacaatggag
     1141 gttccgctgc catcggcaat tcgcttgcgg cggctaccat taatcaacga cggttcttca
     1201 cgttcagccg gggtcatgga tttaataatg gcctcggtgc ttttaaaggc atcatcgttt
     1261 agatcaacat cttttatggc cttacccact ccgggtatca ttccggcaag gtccttcagg
     1321 tttcccattt tctttatctg gcctatctgt tcaagaaaat cgttaaagtt gaacctgttt
     1381 ttaagtatct tcttgttaag ttcccgggct ttatccgcat caaactgttc ctgcgctttt
     1441 tcaaccagcg aaacaatatc gcccattccc agtatgcggt cggccatacg ttcgggatga
     1501 aaaacatcga gggcatcgag cttctcgcct gtgctgataa acttaatggg cttgtcaaca
     1561 acggccctta tggatagagc tgcaccaccg cgggtatcgc catcgagctt ggtgagcacc
     1621 acgccgtcaa agttaagcct gtcgttgaat tcttttgctg tgttaaccgc atcctgaccg
     1681 gtcatggagt caactacgaa tagaatttcc tgaggcgaaa cgaccttttt gatggcggcc
     1741 acctcgttca tcatctcctc atcaactgcc agacgaccgg cagtatcaat tataatcgta
     1801 tctcttccca gcgttttggc ttgtttaata gagagtttag ctatgtcaac gggattactg
     1861 ctacctggaa cggtgaaaat tgggacgcca acctggtcgc caagcacctt aagctgctca
     1921 atagctgccg gtctgtaaac gtcaccggca accagcaacg gcgattttcc ctttttggtt
     1981 ttgaggtagc ttgccagctt ggcagagaaa gttgttttac cggagccttg aaggccggca
     2041 atcagaataa tcgctgggtt tccgttaagg ttgatattgg ctgccttacc acccattagc
     2101 tcagctagct cgtcatgaac tacttttacc attagctgtc caggcctgat ggcattaagc
     2161 acattcattc ccaaggcttt tcgctttacc tcatcggtaa aactcttcgc aactttatag
     2221 ttgacatcgg catcgagaag agctttacga acttccttaa gggtttcggc tacgtttatt
     2281 tcggttattt tcccctctcc cttgaggatt ttaaaggatc gttcgagctt ttcggttaaa
     2341 ttttcaaaca taaattgttt tactatttat tattctctgc aaagttatca aaaatgatgg
     2401 ctttttcaac ataaagccat tttggaagtg cagctaaaag gctatgttta gataaagctt
     2461 ctgttatatc tacataactt ccggcacatc aattcccaga agccacattg cttgtttaat
     2521 aacacgggct actgcttgcg aaagcagaag cctaaagtgt tttacctcga cgttgggttc
     2581 tcgcagtatg ggcaggtctt ggtaaaactg gttgtactgc tttgccagct cgtaggtata
     2641 gttggcaatt tctgctgggt tatgcgtttg agcagctatt ttaattgtat cggggaaaag
     2701 atgtaggagt tgaatgagtt ccagttcctt ttgaaatgaa atgctttcgg gtgttacttt
     2761 ttggttcagt aaatcattca cattgcccgc cttacgaaga acggagcgta tgcgtgcatg
     2821 ggtgtactgg atgaatgggc cggtgttgcc gttgaagtca atagactctc ccgggttgaa
     2881 ggtcatattt tttttggggt caaccttcag gatgaagtac ttaagtgctc ccaggccaac
     2941 cattctgatt gtttctgaag cctcctcatc gctcatctgg tctagcttgc caagttctcg
     3001 cgatacatca ccggcagttt gaagcatttc ggatatgagg tcgtcggcat caaccacggt
     3061 tccctcgcgc gatttcatct ttccttcggg cagcagcacc attccgtagg agaggtggta
     3121 tagagcctcg taccatgtgt agccgagttt tttcataacc agcttaagca cctgaaaatg
     3181 gtagtcctgc tcattgccta ccacgtaaat atgctcgtta aaatggaact cgttgtagcg
     3241 ctctatcgct gtgccaatat cctgggtgat gtaaactgaa gttccgtcgg agcgaagcag
     3301 tagcttttgg tcgaggccgt cggcggtgag atcgacccaa accgaaccat cgctcttcct
     3361 aaagaaaatt ccccggtcca acccctcgtt cactaccttt ttcccaacca ggtaggtttg
     3421 cgattcgtag tatattttgt cgaaggttat tcctagattt tggtaggttt tttcgaaacc
     3481 ttcataaacc caaccattca tcattttcca aagcctgatg gtttcggcat ctttagcttc
     3541 ccattttctg agcatttcct gagcttctat gattataggt gccttggtta atgcctcgtc
     3601 tttgtccatg ccgcttgacg tgagctgctc tacttgcagc ttatactcct tttcaaacct
     3661 gacgtagtag tcacctacca ggtgatcgcc ctttttacct gacgtttggg gtgtttcgcc
     3721 atttccgtac ttaagccaag ctaccatcga tttgcaaatg tggattcccc tatcgttcac
     3781 caggtttacc ttaataaccc ttttcccgct tttctccagt atcctcgaaa tggaaaaccc
     3841 cagaaggttg ttgcggatat gtcccaggtg aaggggctta ttggtgttgg gcgacgaaaa
     3901 ttctacaatg gtagggttag cattttctgt tggggtaggg gcaaatccat agtcttcggt
     3961 tgaggcaatc tcgttgatta cgcctgacca gtacgtttta gatagggtga tattcagaaa
     4021 cccctttatt acgttaattg ctgaaacctc tggatacgat tgaagtagca tttccccaat
     4081 ctggcggcag gtttcttcgg gcgattttcg cgtaagcttc aacagtggaa aggcgaccag
     4141 agtgtagtcg ccttcaaact ccttacgtgt tttttgaatt tggagctggt ttacttccgg
     4201 ttttactccg taaagtttta gaacggtttc cgctacaata tcctgaatgt actgctcggt
     4261 tgacattatt tggttttgtt tgaaatccat gcttcctgat gctgaagtaa taaaatggct
     4321 gtgtttgcaa aagccactgc tccacagcca gaattatacc cgatgttgat aggtagcttt
     4381 agcccgacag gcgagcgtaa aattctactc gtctcttccg tggcagtttt tatatttttt
     4441 cccgctaccg catgggcagg ggtcgtttct accaaccttg atgcctacct taacaggttc
     4501 cctgttgggc tgttgtgttg gtgcacctgc ctgaaggttg tccgttcgtg aggtttgata
     4561 cctactcatg tcggtgtggc tacgttgtct tgcctcctga acctcgtttg cactccgtaa
     4621 cgggataaag gccttggcaa gtatggacac aatgtcgcgg ttcaggtttt cgagcatggt
     4681 gcggaacagc tcgtaggctt caagtttata tatcaacagg ggatccttct gctcgtaggc
     4741 ggcattctgc acgctttgct ttaggtcgtc catttcacga agatgttcct tccagtactc
     4801 atcaatggtg tagaggatta tggattttga aaacgaccta atgagctcct tacctcccga
     4861 ttcgtaggcc tttttcaggt tggtgacaat ctggtaggtt ttttggccat cagaaattgg
     4921 caccacgatg ttctcgtata ttgccgattg tttttcgtaa acatccttaa tgacgggata
     4981 ggcctgcttt gcaatgctgg cagcttttct atcgtacgat tcaagcgctt gatggtatag
     5041 ccgttcggca atttcgtctg gtttaaggtt ttcgtattct tcttctgtaa caggagcttc
     5101 aagggagaaa ttcttgatgg tttccaggga gaagtcttcg tagctttcgc ttcccttgaa
     5161 tgaggaaacc attgcctcgc aaacatccca gaacatgttc gaaatatcaa tatccagcct
     5221 ttcgccgtaa agtgcatggc ggcgacgggt atagatcacc tcgcgctggg cattcataac
     5281 gtcgtcgtat tccagcaacc gcttacgaat accaaagttg ttctcctcaa cttttttctg
     5341 agcccgttca attgacctgg aaatcatggg gtgctggata acctcgcctt cctttagtcc
     5401 cagcctatcc attattctgg caattctgtc agatccaaaa agccgcatca ggtcatcttc
     5461 cagggatacg tagaactgtg aggttccggg gtcgccctga cggccagaac gcccacgtag
     5521 ctgacggtcc acgcgtctgg actcatgccg ctcggtacca atgattgcta gaccacctgc
     5581 agcacgggtt tcgggggtta gcttgatgtc ggtaccacga ccagccatat tggtggctat
     5641 ggtaaccgtt ttcggtagcc ctgcttgtgc cacaatttct gcttcacgct ggtgctgctt
     5701 tgcattcagc acgttgtgct taattccttt taacttcagc attcggctga gcagctccga
     5761 tacctcaacc gaggttgttc ccacaagtac cggcctgttc tcaccggtca gctttacaat
     5821 ttcgtcaata accgcattat acttttcgcg cttagtcttg tataccaaat cctccatatc
     5881 cttacggata acgggttggt tggtaggaat aaccacaaca tcgagcttgt agattgtcca
     5941 gaactcgccc gcttcggttt cagcggttcc ggtcatacct gccagcttat ggtacatcct
     6001 gaagtagttc tgcagggtaa tggtggcaaa ggtttgagtg gcagcttcaa ccttaaccct
     6061 ttcctttgct tcaattgcct ggtgcaatcc atcggagtac cgacgccctt cgagaatacg
     6121 acctgtttgc tcgtcaacaa tttttacctt gttgtccatc accacgtact ccacatcctt
     6181 ttcgaacatg gtgtaggcct tgagcagctg gtgaacggtg tgcacgcgtt cggactttac
     6241 ggagaaatcg cgtaggagct catccttttg tgctaacttc tcgttatggc tgatgggcat
     6301 tttttcaatt tcggcaattt cagcaccaat atcagggagg atgaaaaact tggaatcctc
     6361 cgatgagctg gttattaggt cgttgccctt atcagtaagc tcaacggagt taagcttttc
     6421 gtcaataaca aagaacaggt cgtcggtaat caggtgcatg ttccggttgt tatcctgcat
     6481 gtagtagttt tcggttttaa ggagcagcgc cttgtttccc tgttcgctaa ggaatttgat
     6541 gaggggttta tacttgggca tacccttaaa tgccctgagc agtaattttc caccctcatc
     6601 ggtgttgccc tcggcaatta actttttagc atcggctaaa agctgggtaa tcagggctcg
     6661 ctgagcattt accagctttt ctaccttggg cttgtactcc tcaaacatct ggtcgtcgcc
     6721 tttgggcact ggtcccgaaa tgatgagggg ggttcgtgcg tcgtcaatca gcaccgagtc
     6781 aacctcatcc acaatggcaa agtggtgctt gcgctgcact aaatcatcgg gggcaatggc
     6841 catgttgtcg cgcaggtagt cgaagccaaa ctcgttgttg gttccaaagg taatgtcggc
     6901 gttgtatgct ttccggcgtg cttcagagtt aggttgatgc ttatcgatgc agtcaaccgc
     6961 aaagccgtgg aactcgtaga ttggccccat ccactccgag tcgcggcgag ccaggtaatc
     7021 gttaacggtt acaacgtgaa ctcctttgcc ggccaaggca ttcaggaata cgggaagggt
     7081 tgccaccagg gttttccctt caccggttgc catttcggca attttgccct gatgcagaac
     7141 tactccgcca atcagctgta catcgtagtg aaccatgtcc caaactatct cattaccacc
     7201 ggccatccat cgattcttgt aaatagcctt atcatcctga atggtaacaa agtccttttg
     7261 ggcggctaag tccttatcga attgggtggc tgtaacctcg atggtttcat tttccttaaa
     7321 cctgcgcgcg gtttccttca caatggagaa ggcatccgga agcactatat ttaggatctc
     7381 ctcaatttta tcgtcaatgg tttgctgaag tttctcaaca tccttgtaaa tcttttcctt
     7441 ttcgtcatag ccaatctcgt ctgattccat catttgcttc agacgttcaa cttcggcttc
     7501 atcagcagca atgtaatcgt gcactttagc ctttagtttg gccgattcgc ttctgagctc
     7561 atcattcgat aacgaggggt atttcttgta ggtttcccgt attgtattga gcattggcat
     7621 aagctcccgc atgtcacggt cagcctttgt cccaaatatt tttgataaaa aacttccgcc
     7681 tgccatttta atttaattag tttgaagtta acagtattta aaaccagcaa atttaaccgt
     7741 tttttccgaa taattctgaa tgccggggtt tgattgcgtt aaaaaatact agattggaga
     7801 gccaagtgtt tttactataa tttttttgaa atgggaaagg gtttggccaa caatcaaaaa
     7861 tagtcacata gcctagcatt ctgcttagcg tcgagctcaa aattcgaaca gcttggttga
     7921 gaataaaaaa ttttttgtac agaagtgtca aggcgttccg gtttatttat tactgtagcc
     7981 caagcattaa agttgattgt tgcgcacctg gtttgatgat ggttgctagc gttaatcgtt
     8041 tgtgaactgc aatagccttt gattttccgc taaatattgc gcggtaatgt gctgtttggg
     8101 ctattcgagg tcaaggttta atttttgctt cattttccgg agcgcagggt tatgctgtag
     8161 caaaaattta agcttatcgt cggcggtaaa aagccgtggt ttattctttt cgtctgtttc
     8221 agcaatttcc tctaaaatct cgggtgggtt tacttttagg gattgtttca gaaaggtgag
     8281 caggtcggtt ttaatctccc tgaacatgtc aacctgaatt tgggtttcaa acctgatgta
     8341 agctttgcca tcgctgagaa gttctggacc gtaaagttga agcaggctat actgtcccgg
     8401 tttctcctcc ttaatcgact cggcaaagct actccatgcc ttgccgagca tatcctgggt
     8461 tatagcttta tactcggaat cgttgggcag gttaacagca acactcggaa tttccggttg
     8521 gtcaatatcg ctagcggtgt agctttctac tgtattcttt ttaagcgcac ctttaagcga
     8581 agtggttttg ggaattattg gggttgaagg ttcatttact gcagctggct cgtaggtctc
     8641 atgtttaact ttctgtgctg gttttgtctg tttttgtggt ttttgttcaa ccacatcgcc
     8701 ttcctctatg ttggctgggc tttcagattt ttttttttct tgagtaagcc cgcagagcct
     8761 aatcagcagc aactcaacgt gcagcctttg gttgggactt tgtcggaaag acacatcata
     8821 ctgatttgac agctgcagtg cattaacgag aaaatcgata gggcaagtgg aggccatttt
     8881 ggtgtatttc tcagctatgg cagcacccac ctccagtagc tgaatggtat cggaatcctt
     8941 gcacaccaac aaattcctta aatgctggct taatccggtg ataaattgct gggcatcgaa
     9001 tcccttcgat agtagctcgt cgaatatctt cagcgaatca gtcatgttcc cactcagaaa
     9061 gttatcggtc aacctgaagt agtactgata gtccagcacg ttgagatttt ctattacttt
     9121 ttcgtaggta atatggtttt cgctgaagct aactacctga tcgaagatgg agagggcatc
     9181 gcgcatggcg ccatcagctt tttgggcaat gatgttcaat gcttcaacct cataatcaat
     9241 gccttcagtt ttggcaatgt actcgaggta cttaacggtg tcctcaaccc ttatccggtt
     9301 gaagtcgaaa atttggcaac gcgacaggat ggtgggaaga attttatgct tctcggtggt
     9361 ggccatgatg aaaatggcat gggctggcgg ttcctcgagt gttttcagaa aggcgttgaa
     9421 agcgcttgcg gacagcatgt gcacctcatc aatgatgtaa acgctgtact tcccaatttg
     9481 gggaggtatg cgaacctgct caataagctt gcggataccc tctacggagt tattggaggc
     9541 agcgtcgagt tcgtggatgt taaatgacct gttggtgcta aacgacttgc aactttcgca
     9601 ctcgttgcat gcctcatttt catcggtaag attcaggcag ttgatggttt ttgcaaaaat
     9661 gcgggcacag gtagttttac caactccccg aggaccgcag aaaaggtaag cttgggccag
     9721 ctgtttgcgc tgaatggcgt tttttagggt tgtggttatt gacgtctgac caacgacact
     9781 tttaaatgtt gaagggcggt acttacgagc cgatacaata aagttctcca tgcgttactt
     9841 ccagattgat ggtgcggttt gccatcacaa agttaataca tacaccgaaa ctttcaagcc
     9901 aaaaattagc tgttggccaa tttggctgat aaccagcaaa gtatgcgcta agtcgatcta
     9961 gcttgcttcc ggagtagaat tctgcgggtt accggtgatt tcgacaacca ttacctcgga
    10021 gcgatttcca attttaagcc ttggacccca tgtaccgaaa cccgaagaaa catagtagtg
    10081 ggtgcctccc ttctggtagt atccatggct aacctcatac attgccttgg tgatgtagtt
    10141 gaaaggccac agctgtccgt tatgggtgtg ccccgatagc tgcaggtcaa caccacaccg
    10201 ggctgcatca tccaggttgt agggctggtg gtctaaaagt atggttagtt tccgaaggtc
    10261 aacttccgaa atcagctctt caaccgattt tctctccctt ttcagcatgt agctgctttg
    10321 ccggtcatct cgaccaatga tgtagaaaga gctgtcaacc agcacggttg aatcgcgtag
    10381 cagctttacg ccatgctgct gaaggtattt tatcttttgc tcgaatccgc cataaaactc
    10441 gtggttcccg gtaacggcga aaaccccgta cttagactta aattgctcca gcaaagttcc
    10501 catcccacca tttattacag gtgttacatc ctcatcgaat atgtcgccca ccagcagcac
    10561 cacgtcggga ttttgagcgt tagccatctt cacaagcttt gccagatcac gcttgccaat
    10621 tactgtcccc agatgaatat ctgaaacggc tactacgcgg agtttaccgc cacgcaatgg
    10681 cttgtcaacc ctaacttcaa tctttctaac tatgggggtt atggcgttgg tgtaaccagc
    10741 tattagcaga attgaaacca aagcgtaaac caaggccaca cctacctgcc gggcgtgaat
    10801 cggattagcc gatccaaagg tatgggttga aaccactact atccaaacaa caaggaaagc
    10861 catgaacagg tatagcatgg cacctagcca catggagcct atacgaacaa gcggggtaaa
    10921 tgtatttcca tcaaaaatgg cttctagccc tctccctacg gggtaggcta gcaccagggc
    10981 aagaaagata atttttagca aaatacccca tcggggtatg gagaaaacca cccgggtttg
    11041 atagtaaatg aaaaggttta ccagcaggta aactccaagg gcgatggtaa tgaatacgaa
    11101 catctgaaat ggcttcatcc gtttaaatta tttactgcaa gtgtgagcta atctactgtt
    11161 ctgcccgcga aaatacgttg cataaacata taattgcatt caatgttcac cgtattcata
    11221 aaacatgctg aatcatagct atttgtaatc aagtagattt atcggcaacc taaggcacag
    11281 taggcataaa caggctccag ggttccaccc ggagcgaaat tttacgggca gctgaatcat
    11341 acatagcaag ccctgcctga tactggaatg aatttgctta caggcaggga gtacaaagtt
    11401 tacattcaat ttcgcttaaa gggtaaagtt gcccgaggct tcaggttgat agactatctc
    11461 ctcaattcgt attttggtag gaccggcggg caccagccat tcaatctcat cgcctaccct
    11521 atagccaatt agcgctgtgg caatgggtga aaatatagag attttgttct cctttaagtt
    11581 agcttcgtgg gggtacacaa ttctaaattg aatctccttg ccggtgttca aaaagctgat
    11641 tttgacaatt gagttcatgg taacaacatc tgagggaatg ctttctgggt taactacttg
    11701 ggccgactcc agctccccaa gcagcttatc ggcttcggtg ttggtgattg atttgagttg
    11761 cctggcaact tcaagatgcc tcttaattcg ttcatagtca agtcggttca atgttatcct
    11821 gctcatggtt tcatgatttt agtataattt gaaacgtgat aaagataggc aatgtttacg
    11881 tgtaaatgaa atcttgaggg gaggtgtttt aggtggcacg ctagcttcag ctggatgatt
    11941 tttctgtacc attctgagct actttaccca taacctacat agggttagca ggttataatc
    12001 tggctttgca aaaacctgtt gttaaactag cgattccgcc ctgtgggctg gtgggcgagg
    12061 aaggttttga tcaggtgatc cgggcgggat tagctcgcct gcggctc
//
LOCUS       DAOSHM010000067        12018 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_4486_length_12018_cov_18.831898, whole genome shotgun
            sequence.
ACCESSION   DAOSHM010000067 DAOSHM010000000
VERSION     DAOSHM010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 12018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12018
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4486_length_12018_cov_18.831898"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(81..980)
                     /locus_tag="PK839_08995"
     CDS             complement(81..980)
                     /locus_tag="PK839_08995"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOG72910.1"
                     /translation="MDKRNGTETSAFGTKGRINHDSSKFYSSKLYSEINNNFIGDKAE
                     NQIPNEYENRIILGSAENMKELPNNSVHLMITSPPYNVSKEYDSDLSLSEYLQLLQNS
                     FKETYRVLVYGGRACINIANIGRKPYIPLSDYISRMMIDIGFNMRGEIIWNKASSSSP
                     STAWGSWQSAANPTLRDVHEYILIFSKGDYKREKGDKTDTISKEQFLEWTKSIWTMNA
                     ESAKKIGHPAPFPEELPYRLIQLFSFTDDVVLDPFIGSGTTAISALKSKRRYVGYEIS
                     EEYVNLAERRISPIKQQSVINFR"
     gene            complement(983..1756)
                     /locus_tag="PK839_09000"
     CDS             complement(983..1756)
                     /locus_tag="PK839_09000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72911.1"
                     /translation="MNTGYFAYIPKGTENEGHTLQSRNSLIGQFTEKWCKSILEPIAG
                     KFGLYAINGVVCEEIGLSRQSSADLALCTSNKIIQRPENIKLIFEIKMSIVSNYKYTE
                     PNRIEFISDYKHHKGNPSLLRSDSMLKAIGKSISIRVSGNSSCKIPIVTIGNSPISKN
                     YVDKIDFLKSSGVIQGFWSLNPNPCNSEFIKETPQKGFQTFKNQKMITESCKELLESD
                     MLYFSSMVSMPKLGEIIRLANKEKTDAAKAEKFLTLIRG"
     gene            2008..2430
                     /locus_tag="PK839_09005"
     CDS             2008..2430
                     /locus_tag="PK839_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOG72912.1"
                     /translation="MEKVAIQAEWLEGLAFETEVNGHKLVVDADENAGGSNRGPRPKP
                     LMLVALAGCTGMDVISILKKMRVDVKRFSVGVEAMQTDEHPRHYVGMNIIYEFWGKDL
                     PPESINKAINLSQEKYCGVSASYRKAMPITFEVKIHEE"
     gene            2444..2653
                     /locus_tag="PK839_09010"
     CDS             2444..2653
                     /locus_tag="PK839_09010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72913.1"
                     /translation="MNITGNTLIEDLVEQLPESVSFLREKGIICIVCGEAVWGTLAEV
                     AQQKGLSDEAIEATIEELNQLKKSK"
     gene            3015..4034
                     /gene="asnA"
                     /locus_tag="PK839_09015"
     CDS             3015..4034
                     /gene="asnA"
                     /locus_tag="PK839_09015"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005843676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HOG72914.1"
                     /translation="METPAKLYQNVLDARQTEDAIALIHDFFPGKLAAVLNLRRVTAP
                     LIVPSGMGINDDLNGVEQPVRFSAKSLGNREVEVVQSLAKWKRITLARLKVEPGKGIY
                     TNMNALRPDETLDATHSIYVDQWDWEKCIADAQRNLEYFRRTVEQIYSAMKDLESHIF
                     EHFPQLAPVLPDKIHFIHTSELEARFPNLSHRQREYESCRELGAVCIEGIGYPLSNGH
                     PADGRAPDYDDWSTLRGDGKRGLNGDILVWHPTLQNSLELSSMGIRVNSEALKLQLAM
                     QDCDNRRGLWYHRQLLEDKLPQSIGGGIGQSRLCMFLLRKSHIRDVQASVWPDECCAS
                     NNAMM"
     gene            4234..5019
                     /gene="truA"
                     /locus_tag="PK839_09020"
     CDS             4234..5019
                     /gene="truA"
                     /locus_tag="PK839_09020"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HOG72915.1"
                     /translation="MPRFKLTIEYDGSHYRGWQFQREMPTVMGKLMDAIKEVYKVHEY
                     ELYGSGRTDGGVHALAQIAHLDIESDLPPHMAKSRLNEELPSSINILNIEKVSPRFHA
                     RYSAIARSYVYHISKRRTAFGKDYTWWVKDELNVKDMADATTLFTGMKDFRSFGMAEK
                     DDSSTLVKLQTVKLFEVDDSILVHIVGSHFLWKMVRRVVGTLVEVGRGSITGDDIQRF
                     FSEYSEQPARFTAPPSGLYLERVYYPGDKIADEPEWLMNISHH"
     gene            complement(5183..6724)
                     /locus_tag="PK839_09025"
     CDS             complement(5183..6724)
                     /locus_tag="PK839_09025"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007561317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HOG72916.1"
                     /translation="MRSLEKLYSELEARNQAAEMAGGQKRIDKQHEGGKKTARERIVE
                     LLDPGTFTEIDKFVTHRTVEFGMADKHIPGDGIVSGYGKIDGRRVYVYAYDFTVFGGT
                     LSRANADKIIKLQQLALKMGVPVIGLNDSGGARIQEGVQSLAGYAEIFYNNVRSSGVI
                     PQITCIMGPCAGGAVYSPALTDFIVMVKDTSYMFVTGPDVIKAVTHEDVTKDELGGAV
                     THNSKSGVAHFIADNDEQALMLVRELMSFLPINNMEEPPIVPTTDDSTREDELLQTLI
                     PADPNKPYDIKDLILSVVDDRNFLEVQALFAKNIVVGFARMAGHTVGIVANQPAHLAG
                     VLDIDASVKAARFVRFCDSFNIPLVTFVDVPGFLPGVNQESNGIIKHGAKLLYAYSEA
                     TVPKIAVITRKAYGGAYCVMSSKQVGADVNFAYPMAEIAVMGPDGAVNILYRDRIDNE
                     EVRLQLIEEYRETFANPYRAAELGYVDEVIYPKQTRYKLIQALEMTRSKSQPTPPRKH
                     GNIAL"
     gene            complement(6805..7314)
                     /locus_tag="PK839_09030"
     CDS             complement(6805..7314)
                     /locus_tag="PK839_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008153136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HOG72917.1"
                     /translation="MGLEVKIGNRVADVKLVKREGQQVTIEVDGKLYEVDIAQVENGV
                     YSILHNGKSYNIEIIPGDGPKSYFVNTFYNSHEVEIIDAHSRYMMSRNLSSENAADRV
                     ISTPMPGRVVKIPVTEGEQVSKGATVIVISAMKMESEYKSPVDGTIRKIHVAEGDNIA
                     ANQPLIEIE"
     gene            complement(7325..8839)
                     /gene="accC"
                     /locus_tag="PK839_09035"
     CDS             complement(7325..8839)
                     /gene="accC"
                     /locus_tag="PK839_09035"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008144416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HOG72918.1"
                     /translation="MIKKVLIANRGEIAVRVIRTCREMGILTVAVYSDADRKALHVRY
                     ADEAYYIGPSPSRESYLNGDRIIEVALRSGANAVHPGYGFLSENADFARKCAEAGLIF
                     IGPDPWAMTQMGDKITARQTMIKAGVPVVPGTQQKVDDIQAVRRIVDEIGLPIIIKAS
                     AGGGGKGMRLVRQPSELVSALKMAQSEALSSFGDDTVYIEKYVEAPHHIEFQILADKF
                     GNTVHLFERECSIQRRHQKVVEETPSPFMTPELREKMGQAAIAAAKAVNYTGAGTIEF
                     LVDAERNFYFLEMNTRLQVEHPITERVTGVDLVKEQIRIASGMPLSFSQNQIHQHGHA
                     IECRVSAEDPFNNFMPSPGLVRHISEPNGLGIRTDGYVYEGYEIPIHYDPMISKLISW
                     GRTREEAVDRMKRALFEYKITGVKTSLRFLSRIMENKDFRNGVYNTHFVPDHFDELIG
                     GEATEKPIYEDMAVIAAYFDFTSKMERVAMNDFSIQHKPDSWKVYGINRNLMRI"
     gene            complement(9215..9943)
                     /locus_tag="PK839_09040"
     CDS             complement(9215..9943)
                     /locus_tag="PK839_09040"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2807 domain-containing protein"
                     /protein_id="HOG72919.1"
                     /translation="MFYKKFTYLNLLLLFILGCSTNEIIDPGDVETRRVEISKSYRSI
                     EVENTFEITLVQDSLSYALVTCGEKLQQDVSIMVKDSILFLKNNLNLHFLKGYDKVKV
                     ELHLNEIPITNIRKPSKIQTAGVFRTPTFYLVDWNSFTDCNVNLEVDNLILSISGSNF
                     GSYHVEGKAISASLFCEGTALFDLANMSIDSCCVSHDGLNDVFLHVNRYLSATFTSKG
                     NVYYSGEPICLVSGDGSGRLIKVN"
     gene            complement(9984..11120)
                     /locus_tag="PK839_09045"
     CDS             complement(9984..11120)
                     /locus_tag="PK839_09045"
                     /inference="COORDINATES: protein motif:HMM:NF020965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HOG72920.1"
                     /translation="MGWFGKGLRLKVAFSAMGLLAVITGADAQDAPIRDRLFFGVEPR
                     WGFVMPHHDYMAYFLESNVNSFQLNVGLNTTGYKDWHKSFNYPQLGGGFFYSGLGNDR
                     IYGKMLAAYVFIDRQFLPRKNRFNIGNRAAFGMSYLTKQFDLHSNPYNMVIGTPFNVF
                     FQYDLTLYYRLNSNLNLRWALGFSHASNGNLREPNKGFNLITSGFGLQYSISDDRLLV
                     TQAPGYHSDSTRTYVSIGVLGSAKSVSRENTNLYPVYGMSVEWLRRLSKTSLAGVELA
                     AYKDKAIKYTYQAKDDSTYRSSDSYAVTLNPAYIMQLGRTMFVFQPGIYLKRGYKPDG
                     YITNKVGMRFQLSQNLYASWAIKAHWAAKADFVEFGIRYRIIAD"
     gene            complement(11132..11746)
                     /locus_tag="PK839_09050"
     CDS             complement(11132..11746)
                     /locus_tag="PK839_09050"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HOG72921.1"
                     /translation="MKKPGKLCVILIISACPAVVFSQGFKAGFTLGIAATQVDGDTYG
                     GFDKAGPVAGVWVGRNLGNSWLGMMELRYAQKGSYAKTTVNEFNGYYRMRLHYFELPV
                     MGGYRFVNGFQTLGGISLGYLAKAEEMTELGPFPEEDISAFNQFELAMLCGVEYNYSE
                     HWAFGAFFSYSIFPIRPYKGNITYRMNRGQYNQVVELLVRYKIQ"
BASE COUNT         3339 a         2841 c         2606 g         3232 t
ORIGIN      
        1 aagcattcaa gtaaagctgg atactccact tcactcaaaa gccctgccaa cctttgctgg
       61 cagggttttt tccataaaac ctacctgaaa tttataaccg attgctgctt aataggggaa
      121 attctccttt cggccagatt cacatattct tccgatattt catacccaac atatctcctt
      181 ttcgatttta aggcgctaat agcggtagta ccactcccga taaatgggtc caaaacaaca
      241 tcatcggtga aagaaaatag ctggattaat ctatagggta attcttcagg aaatggggca
      301 gggtgtccta ttttctttgc gctttcagca ttcattgtcc aaattgactt tgtccactcc
      361 aggaactgct ccttggatat ggtgtccgtc ttgtcccctt tttctctttt gtagtctccc
      421 tttgaaaaaa ttaatatgta ttcgtgtaca tctcttaatg taggattggc ggcgctctgc
      481 caactccccc atgctgtaga ggggctagaa cttgaagctt tgttccatat tatttcgccg
      541 cgcatgttaa atcctatatc aatcatcatt ctcgaaatgt agtcagagag aggaatatat
      601 ggcttacgcc caatatttgc aatatttata catgctctac caccatatac cagcacccga
      661 taggtttcct taaatgaatt ttgcaaaagt tgtaagtatt cgcttaacga taaatcgcta
      721 tcgtattcct ttgaaacatt atagggaggc gaggttatca ttaaatgaac cgaattatta
      781 ggcagttcct tcatattttc agctgaaccc agtatgatcc ggttttcgta ttcgttgggg
      841 atttgatttt cagccttatc acctataaaa ttattattta tctcggagta tagcttcgag
      901 ctatagaact ttgaactgtc atgattaatt ctacccttag ttccaaaggc gctggtttct
      961 gtcccatttc ttttatccat agctatcccc ggattaatgt taaaaatttt tcggctttcg
     1021 cagcatctgt cttctctttg ttagccaatc taataatttc accaagtttt ggcatagaaa
     1081 ccattgatga aaaatagagc atatcgcttt ctaacaattc cttacaactt tctgtaatca
     1141 ttttctggtt tttgaatgtt tgaaagcctt tttgcggagt ttctttgata aattcggaat
     1201 tgcagggatt agggtttagc gaccaaaaac cttgaataac acctgaagat tttaaaaaat
     1261 caattttatc aacataattt ttgctaattg ggctattgcc aatagtaaca atgggaattt
     1321 tacacgagga atttcctgaa acccgaatgc taatagattt tccaatagct ttgagcatag
     1381 aatcggaacg gagcaatgaa ggattaccct tatgatgctt gtagtcgcta ataaattcaa
     1441 ttctgttcgg ctcagtatat ttatagtttg aaacgatact catcttaatt tcaaaaataa
     1501 gcttgatgtt ttcgggacgt tgaatgattt tattcgaagt gcaaagcgcc agatcggcag
     1561 atgattgcct cgataggcca atttcttcac atacaacgcc attaatggcg tacagtccaa
     1621 atttaccagc tatgggttca aggatagatt tacaccattt ctcggtaaac tgcccaatca
     1681 gcgaattcct actttgcagg gtgtgcccct cattctccgt acctttagga atatatgcaa
     1741 aataacctgt attcaacttg taaaatagct tttccggaga ggcaaaattc ttcagtgctt
     1801 cctcaaaaaa ttgaatttca atatcgttat tccacagaat cattcgcaac acatttttac
     1861 aaatttagca attatcaagc atatagaagt tgcttacatg atttctttta gcaccccgct
     1921 ggtgctttac cctttcatac ctgatctgta aaaatttttt tgaactttac cccaaaaatt
     1981 cggttcttac atatcaaaac taacactatg gagaaagtag ctattcaggc cgaatggttg
     2041 gaaggcttgg cctttgaaac cgaggtaaac ggccacaagc tggttgttga tgccgacgaa
     2101 aatgccgggg gaagcaaccg cggcccccgc cctaaaccgc ttatgctggt tgccctggcc
     2161 ggctgcacgg gaatggatgt aatttctatc cttaagaaaa tgagggttga cgtaaaacgg
     2221 ttcagcgttg gcgttgaggc catgcaaacc gatgagcacc ccaggcacta tgttgggatg
     2281 aacataatct acgagttctg gggaaaggat cttccgcccg aaagcatcaa caaggccatc
     2341 aacctgtcgc aggaaaagta ctgtggggtt agcgcctcat accgcaaggc tatgccaatt
     2401 actttcgagg taaagattca cgaagagtaa caggagaata aggatgaaca taaccggcaa
     2461 taccctgatt gaggacctgg tggagcagct acccgaatcg gtttcgttcc ttagggaaaa
     2521 aggaattatc tgcatagttt gcggagaggc tgtatgggga acacttgctg aggtagccca
     2581 acaaaaggga ctttccgacg aggctattga agcaactatc gaggagctaa accagctaaa
     2641 aaaatcaaag taaattttga gaatttaaag gaaaatccta ccttgccctt gaaaatttgg
     2701 atgaaatgat tcacatattc cgcaataatt gcaattacaa tttcaacaat aatggctggt
     2761 tgttgaaagg ggattgctat tgctgaaata aacattgatt acccgcaaaa ggctttccca
     2821 aaaagggaga gccttttttg attgcagtaa cccaactaga agtaacgcca tgcaaacccc
     2881 gctactaaac ggaaataata ataaccgccc acctccctcc gggtcaggat catgctgtat
     2941 ttgcccttcc cggaaaaacc gtgtgaaggg ttttcttttt atgcaagttc ctcaacctaa
     3001 aaaaaataaa ttacatggaa acaccagcta aattatacca aaacgttctg gatgcccgcc
     3061 agacagaaga tgcaatcgcc ctcatccacg atttctttcc cgggaaattg gcagcagtgc
     3121 taaacctacg gcgtgttacc gcaccgctga tagtaccctc gggtatgggt ataaacgacg
     3181 acctcaacgg ggttgaacaa cctgtcaggt tcagcgccaa atcgctgggc aacagggagg
     3241 ttgaagttgt gcagtcgctg gccaagtgga aaaggataac ccttgccaga cttaaagtag
     3301 agccaggaaa gggtatctac accaacatga atgcactccg acctgacgaa acgctcgatg
     3361 ccacacattc aatttacgtt gaccagtggg attgggaaaa atgtatagct gatgctcaac
     3421 gcaacctgga gtacttcaga agaacggttg aacaaattta ctccgccatg aaagatttgg
     3481 aatctcatat ctttgagcac tttccccaac tagcgccagt tttgcccgat aaaatacact
     3541 tcatccacac cagcgagctg gaggcaaggt ttccaaacct ttcgcaccga caacgcgagt
     3601 atgaaagctg cagggaactt ggagctgtat gtatcgaggg aattggctat cccctatcga
     3661 atggccatcc tgcagatggt cgggcacccg actacgacga ctggtcaacc ctccgaggtg
     3721 atggtaaaag aggcctcaac ggtgatatcc tggtttggca tcccaccctt caaaattcac
     3781 tggagctctc gtctatggga attagggtta acagcgaggc gcttaagctg cagctggcga
     3841 tgcaggattg cgataatcgt cgcgggcttt ggtaccaccg ccaactactc gaggacaagc
     3901 tgccacaaag cattggggga ggaataggcc aatcgcggct ttgcatgttt ttgctacgaa
     3961 aatcacacat acgggatgtt caggcctccg tttggcccga cgaatgctgt gctagtaaca
     4021 acgcgatgat gtagaaaccc catattacct taaaggtgtg aaccggtagg tagtggaata
     4081 ctactgaaaa tagcctcaaa tatattataa tcagcttgct gaaagccacc ctgcatggct
     4141 ggcagtaacc tattggcagc tgcacatggc tcacctttag caccctaaac ttctacagct
     4201 gggaataaag ctgtatattt gtaacaaaaa attatgcccc ggttcaagct gacaattgag
     4261 tatgatgggt cccattaccg tggatggcag tttcaacgcg agatgcctac ggttatgggt
     4321 aagctgatgg atgccattaa ggaggtttac aaggtacacg aatacgagct gtacggttcg
     4381 ggacggactg atggaggcgt tcacgctctg gctcagatag cacacctgga tattgaatct
     4441 gatcttccgc ctcacatggc aaagagcagg ctcaatgagg agctgccctc cagcatcaac
     4501 atacttaaca tcgaaaaggt tagcccccgt ttccacgccc gttacagcgc tattgcccgt
     4561 agctatgttt atcacatatc taaaagacgt actgcttttg gtaaggacta cacctggtgg
     4621 gtaaaggatg agctcaacgt aaaggatatg gccgatgcca caaccctttt cacggggatg
     4681 aaggacttcc gatcatttgg aatggcagaa aaggacgaca gctcaaccct ggttaagctg
     4741 caaacggtca aattatttga ggttgacgat tccatactgg tgcatatagt tggctcgcac
     4801 ttcctttgga aaatggttcg tcgagttgtg gggacactgg ttgaggttgg aaggggaagc
     4861 atcaccggtg atgatattca gcgatttttt tctgagtact ccgaacaacc tgcccggttc
     4921 accgcacctc catcgggact ctacctggaa agggtttact acccgggcga taaaattgcc
     4981 gatgagcccg aatggcttat gaatattagt caccactagc ctttactgtg ctgaaaaaaa
     5041 gtaaacgcct gacttaacaa gttcaggcga ttatttttta aaagagaagt ggctacgtta
     5101 ctcttgtaaa aagatgaacg ctcacatttc aacatttaaa gattcacata taaagccctt
     5161 attaagaatg ggataagcct tcctaaagag caatatttcc atgcttgcgt ggcggagtag
     5221 gctgactctt actgcgggtc atctccagtg cctgtatcag cttgtaccgg gtttgcttag
     5281 gatagataac ctcgtcaaca tatcccagct cagctgccct gtaggggttg gcaaatgttt
     5341 cacggtactc ctcaatcagc tgtaggcgaa cctcctcgtt atctatccgg tcacggtaga
     5401 gaatattaac agccccatcc ggccccataa cggcaatttc ggccatgggg tatgcaaagt
     5461 taacatcagc accaacctgc ttgctcgaca tcacgcagta agcaccacca taagccttac
     5521 gggtaataac ggctattttg ggaacagttg cctcggagta ggcatagagc aacttcgctc
     5581 catgcttgat aatgccgtta gactcctggt tcactcccgg tagaaatccg gggacatcca
     5641 caaaggtaac cagcggaatg ttgaaggaat cacagaagcg gacaaatcgg gcagctttaa
     5701 ccgaagcatc aatatcgaga acccccgcca ggtgggcagg ctggttggct acaatgccca
     5761 cggtgtgtcc ggccatccgg gcaaaaccca ccacaatatt ctttgcaaaa agagcctgaa
     5821 cctccaggaa gtttctatcg tccaccaccg acaggatcaa atcctttata tcgtagggtt
     5881 tattcgggtc ggcgggaatc agggtttgca gtagctcatc ctcgcgggta gaatcgtcgg
     5941 ttgttggcac aattggaggc tcctccatgt tatttatggg cagaaaactc atcagctccc
     6001 gcacaagcat tagggcctgc tcgtcgttgt cggcaataaa gtgggccacc ccgcttttgg
     6061 agttgtgggt aaccgcaccg cccagctcgt ccttggtaac atcctcgtgg gttacagctt
     6121 taatgacgtc ggggccggtt acaaacatgt agctggtgtc ctttaccatc acgatgaaat
     6181 cggttagagc gggtgagtaa acagctccgc ctgcacacgg ccccatgatg caggttattt
     6241 gaggaattac cccggagctc ctcacgttat tgtagaatat ttcagcatat cccgcaagac
     6301 tttgcacgcc ttcctgtatg cgggcgcctc cggaatcgtt gagcccaatg accggaacac
     6361 ccatttttaa ggccagctgc tggagcttga taatcttgtc ggcattggca cggctcagcg
     6421 ttcctccaaa taccgtgaag tcgtaggcat aaacgtaaac ccgtcgcccg tcgattttac
     6481 cgtatccgga tacgattcca tcaccgggta tatgcttatc ggccatgccg aattctactg
     6541 tccgatgggt tacaaacttg tctatttcgg tgaacgttcc cggatcaagc agctcaacaa
     6601 tacgctcgcg ggcggttttc tttccaccct cgtgctgctt atcaatacgt ttttgaccac
     6661 cggccatttc agccgcctgg ttacgagcct ccagctcgct gtaaagtttt tctaaagacc
     6721 tcataaaaag atggttaaat ttggcaggta gtagcttgac ctaccgcaaa atgaatcata
     6781 aagaataagt agcccttaat agagctactc aatctcgata aggggttgat ttgcagcgat
     6841 attatcaccc tcggcaacat gaatctttcg gatagttcca tccacggggc tcttatactc
     6901 gctctccatt ttcatggccg aaattacgat taccgttgct ccttttgaca cctgctcgcc
     6961 ttcggttaca gggattttaa ccaccctacc gggcattggt gttgaaataa ccctatctgc
     7021 tgcattttca gacgataggt ttcggctcat catgtaacgg ctgtgggcat ctataatctc
     7081 aacctcatgc gagttgtaga aggtattcac aaagtaactt ttaggaccat cgccagggat
     7141 aatttcaatg ttgtacgatt tgccgttgtg caaaatggag taaactccgt tttccacctg
     7201 ggcaatatcc acctcgtaga gtttcccgtc aacctcaatg gtaacctgct ggccttcccg
     7261 tttaacaagc tttacgtcgg caacccgatt gccaattttt acctcaagtc ccatcctctg
     7321 aaagttatat gcgcatcaag tttctattaa taccgtaaac cttccagctg tcgggcttat
     7381 gctgaatgga aaagtcgttc atggcaacgc gctccatctt ggaggtaaaa tcgaagtagg
     7441 cagcaataac ggccatatcc tcatatatgg gtttttctgt agcttcaccg ccaatcaact
     7501 cgtcaaaatg atcgggtaca aagtgggtgt tgtacacccc attgcggaaa tccttatttt
     7561 ccattattct ggaaaggaag cgaagcgagg tctttacacc ggtaatctta tactcaaaaa
     7621 gtgcccgctt catgcggtca accgcctcct cgcgagttct accccacgaa atcagcttgc
     7681 taatcatagg atcatagtga atggggatct cgtatccctc gtaaacataa ccatcggttc
     7741 taattcccag cccgttgggt tcacttatat gccgcactag tccgggtgat ggcatgaagt
     7801 tattgaaggg atcttcagca gaaacccgac attcaattgc atggccatgc tgatggattt
     7861 ggttctggct aaacgaaagc ggcatacccg atgcaattcg gatttgctcc tttaccaggt
     7921 caacccccgt aaccctttcg gtgatgggat gttcaacctg caaacgggta ttcatctcca
     7981 ggaagtaaaa gttgcgttcg gcatcaacca ggaactcaat agtccctgcg cccgtataat
     8041 tcacggcttt ggctgctgct atagcggcct gccccatttt ctcacgcagc tcaggagtca
     8101 taaaaggcga tggggtttcc tccacaacct tctggtgcct gcgctggatg gaacactccc
     8161 tttcgaacag atgaaccgta ttgccaaact tatcagctag tatttgaaac tcaatatggt
     8221 ggggtgcttc aacgtacttc tctatgtaaa ccgtatcgtc gccaaacgag gaaagcgcct
     8281 ccgattgtgc cattttcaga gccgacacca gctcggacgg ttggcgtaca agcctcattc
     8341 ctttaccacc tcctccggca gaagccttaa tgatgatggg cagcccaatt tcatcaacaa
     8401 tacgccgaac tgcctgaatg tcgtcaacct tttgctgggt tccgggaaca acgggaacgc
     8461 cggcttttat catggtttgg cgggcggtaa ttttatctcc catttgggtc atggcccagg
     8521 gatcggggcc aataaaaata agaccggctt ctgcgcactt acgtgcaaag tctgcattct
     8581 cggacaaaaa accatagccc ggatgaacgg cattggcgcc gctacgtagc gccacttcaa
     8641 taatcctgtc accattcagg tagctttctc ttgagggcga aggtcctatg tagtaggcct
     8701 cgtcggcgta gcgaacatgc aaggctttac ggtcggcatc ggagtaaacg gccacagtaa
     8761 gaatgcccat ttcccggcat gttcttataa cccggacggc aatttcgccc cggttagcaa
     8821 ttagcacttt tttaatcata agtcccagat ttgaatccgc tgcaaatgta aacactttta
     8881 tatgttatat aacatatttt attaattttt tatcgaaaaa aatgctataa tttttttaaa
     8941 attttaacgc aatcgtttta aatataagga aagatggcca ttgcagggat aaaaaaatta
     9001 taaacatggt gccaggatac gagaaaatca tcaatcaaag aaatttttct ccatataatg
     9061 ttatctattt aattactaac tgtttaaaat ataataattt accagaagca tcaaattatg
     9121 tttagttttt attgaattat aatgaggtag aaacagtcat gcttaaatta cctggggaaa
     9181 atcaaagcca tgagcaagaa catccaccaa tgtgctagtt taccttaatc agcctgccac
     9241 ttccatctcc tgacaccaaa caaattggct cgcccgaata gtacacgttt cctttcgacg
     9301 taaaggttgc gcttaaatac ctattaacat gtaggaaaac atcattcaac ccgtcatgcg
     9361 aaacacagca agagtctatg ctcatgttcg ccaggtcgaa cagggctgtt ccctcgcaga
     9421 aaagcgaggc gctaatggct ttcccctcca catggtagct gccaaagtta gaacccgaaa
     9481 tactcagtat gaggttgtcc acctcaaggt tgacgttgca atcggtaaaa ctattccagt
     9541 caacaaggta gaacgttggt gtacgaaaaa caccagccgt ttgaattttg ctgggttttc
     9601 gaatgttagt aataggaatt tcgttgaggt ggagttcaac tttaacctta tcgtatcctt
     9661 taaggaagtg caggttaaga ttattcttca ggaaaagaat gctatccttt accataatgc
     9721 tcacatcctg ctgaagtttt tcgccacagg ttaccagagc ataggaaagc gaatcctgaa
     9781 caagggttat ttcaaaggta ttctccactt caatggacct gtagcttttc gaaatttcaa
     9841 cacgtcgggt ttcaacatcg cccgggtcaa taatctcatt cgtactgcaa cccaaaatga
     9901 aaaggaggag cagatttaag taggtaaact ttttgtaaaa catgataatt aacctttata
     9961 ttaaacaatt gcctgcagaa tgattaatcg gcaatgattc tgtaccgaat tccaaactca
    10021 acaaaatcgg ctttggcagc ccaatgggct ttaattgccc acgaagcgta taggttttgg
    10081 gagagctgaa agcgcattcc aaccttgttg gtaatgtacc cgtcgggttt gtagcctcgt
    10141 ttcaggtaaa ttccgggctg gaaaacaaac atagttcttc ccagctgcat aatgtaggcg
    10201 ggattgagag taactgcata ggaatcggat gacctgtatg tgctatcatc cttagcttga
    10261 taggtgtact taattgcttt atccttatag gcagcgagct caactccagc cagagaggtt
    10321 ttgctcaaac gtcttagcca ttcaacggac atgccgtaaa cagggtagag gttggtattt
    10381 tcacgagaaa cagatttggc acttcccaga actccaatgc taacgtatgt tcgggttgaa
    10441 tcggaatggt agccgggggc ctgtgttact agtaacctat cgtctgatat ggagtactgc
    10501 agtccaaaac cgctggtaat caggttaaaa cccttgttgg gttccctcag gttgccattg
    10561 gaggcatggc tgaagcccag tgcccaacgt agattcaggt tggagtttag gcggtagtaa
    10621 agggttaaat cgtactgaaa gaaaacgttg aaaggagttc caataaccat gttgtagggg
    10681 ttggaatgca aatcaaactg cttggtgagg tagctcatcc caaatgcagc ccggttgcca
    10741 atattaaacc tatttttccg gggcaaaaac tgtcgatcga taaaaacgta ggctgcaagc
    10801 attttaccat agatacgatc gttgccaagc ccggagtaga agaacccgcc acccagctgc
    10861 gggtagttga aactcttatg ccagtcctta tatcccgtag tgtttagccc aacattcagc
    10921 tgaaagctgt tcacattgct ctcaaggaag taggccatgt agtcgtgatg gggcatcacg
    10981 aatccccatc gcggttcaac tccgaaaaaa agcctatccc taataggagc atcctgcgca
    11041 tctgctccag tgataacagc cagcaatccc atcgccgaaa atgcgacttt tagccttaaa
    11101 cctttaccaa accaacccat agcacaatga tttactgtat tttatatctt accaataatt
    11161 ctacgacctg gttgtactgc cccctgttca tcctgtaggt aatatttccc ttatagggac
    11221 gaatgggaaa gatagagtag ctgaagaatg ccccgaaagc ccaatgttcg ctatagttat
    11281 actccacacc gcacagcatg gccagttcaa actgattaaa tgccgagata tcctcctcgg
    11341 gaaaggggcc gagctcagtc atttcctccg cctttgctag gtatcccagg ctaattccac
    11401 caagcgtctg aaagccattc acaaagcgat acccccccat aacaggtagc tcaaagtagt
    11461 gaagcctcat ccggtagtaa ccattgaact cattaactgt tgttttggca tagcttccct
    11521 tttgagcata gcgtaattcc atcatgccca accagctgtt accaaggttt cggcccaccc
    11581 acactccggc caccgggcca gccttatcaa aaccgccgta ggtgtcgccg tcaacctggg
    11641 ttgcagctat accaagagta aaacctgcct taaacccttg tgaaaacacc acagctgggc
    11701 aagccgaaat aatcagtata acgcaaagtt ttcctggttt tttcaattct taataccttt
    11761 ttattaatac caccaatgca cgactaccca tagcagctgg aaaaaactga tttccaacag
    11821 gaggcataat aagacccagg ctttccgcca atgcaactcc tcctgaatga aatacataaa
    11881 aatttgaaaa ggaaaacaaa agtagtaaat cttccgcttt tagacgatag ctaagaaacc
    11941 aaatgttttc gtatgagggg attactcccc caccctctca caccacccgg cttccgcctg
    12001 cggcgggcac ggcggttc
//
LOCUS       DAOSHM010000068        11900 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_4531_length_11900_cov_12.191389, whole genome shotgun
            sequence.
ACCESSION   DAOSHM010000068 DAOSHM010000000
VERSION     DAOSHM010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 11900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11900
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4531_length_11900_cov_12.191389"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="PK839_09055"
     CDS             complement(<1..332)
                     /locus_tag="PK839_09055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72922.1"
                     /translation="MNFTRGALFAISSIIVSSSFTLAHGSSDTISVRKSVEAFRIETQ
                     LDINGHLDEPEWQLAGFAESFTQYEPYNGASPSQPTRVKILYDDDALYIGAWLFDSSP
                     DSIYKELG"
     gene            complement(498..1514)
                     /locus_tag="PK839_09060"
     CDS             complement(498..1514)
                     /locus_tag="PK839_09060"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOG72923.1"
                     /translation="MKSSQITIKDIARELGISPSTVSRALKDHPDISQETKRVVNELA
                     AKYNYKPNIIALSLRNQRSNVIGLVIPEIAHYFFSTVISGIEEVAHAHGYSVMVNQSG
                     EDYKREVAACDAFLNGIIDGLIVSVSKETENYEHFQRFEDEGIPLVFFDRIIEEISAD
                     RIIIDDFDGAYQATEHLIIQGRRKIVHFSGPQNRLIGQNRLNGYLKAMRDNGVVIDER
                     LIIHCDTFQSALIETQKLIDSGTKFDSIFTVNDFTAAGAMKILIRNGIKIPQDVSVVG
                     FGDDQTSLMVEPTLTTVNQPGFEMGKKAMEQLIRRITQTRQEPPITEILKTQLIVRES
                     SRLR"
     gene            1793..4747
                     /locus_tag="PK839_09065"
     CDS             1793..4747
                     /locus_tag="PK839_09065"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOG72924.1"
                     /translation="MIRFRQNLLVVAALLLGSLSLMAQEITVTGKVTDATDGTPMIGV
                     AVVVKGTTTGTITNVDGEYTIRCKGTDVLAFSFVGYNTLEIEVANRNIVNVQMKQETK
                     EIDEVIVIGYGVQKKSDRTGSVVNVKAEEMNRGSLTDPIQAMQGKVAGVNISKKGGDP
                     NSGFAVQIRGASGFASNTQPLYVIDGVPGADPTTISPDDIESFNVLKDASSGAIYGSR
                     ASNGVIIITTKKADKSKGAIIDYNGYVSADQTSKRMKFLSADDIRKYVTDNNIPDFAD
                     NGANTNWQDEIFKTGISQSHSIAVSNSTDNLSYRFSYNHTYNDGVVIQSNKQRDIARF
                     NLTQKALNDIVTIDASIAGTFEKNNYVNYGGGMGSSNIFYQMYQRNPVDPVYKDGKFF
                     EFQRDFNYNNPVAIAKLIQNERDAKNYFGNAKISVEPIKGLVGSVNFGYIRNDSESFY
                     FEPTNLYAGGTDGYGKRGYSNFSSQVLETTISYNIVLEDHSINLLGGYSFQEDNNSGL
                     SAQGREALSNYLKSNNLGHLNNVNVGDIGSWRNSNRLISFFARATYNYQSKYFATATI
                     RRDGSSKFGKNNEWGLFPSVSIAWDIKKEAFLENIDLFNQLKLRVGYGITGNQEISSF
                     LDIMYASPSGTAPNFETGEDAINFAISHNANPDLKWEENSELNIGLDYSILKNRIQGS
                     FEYYIKSTYDLLAPYAVPVPPNALPTTWANVGQIDNKGFEANITGYAIDNKYFKWQTI
                     VNLSTNHQKLVSLSNDQYSWSEADKKRLWLSGRGLVGAQNWTQYLFEGEEIGTFYIPK
                     YAGLSNDGKFLFYTAAGGVTRDVNLAQRYVAGYAQPRFTLGWSNLFTYKNFDFSIALR
                     GAFGNKVMNVTHMVFSNPQILPTLNALSEVLDEIDRGLTDSPKVSDYYLEDASYIKVD
                     NISIAYNFNPKSTKWVKNMRVYLTSNNLYTFTKYTGLDPEQNYVGLEYGLDMYDVYPK
                     TRTVTLGLDIKF"
     gene            4767..6257
                     /locus_tag="PK839_09070"
     CDS             4767..6257
                     /locus_tag="PK839_09070"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOG72925.1"
                     /translation="MKRYFILTVTALMALTSCTDIDDQLTDKIPFDQFPENDAQSALM
                     VVPVYRPMQDFLDWSGWWFCQEVTSDEMVCPTRHTDWDDGGKWRVLYQHTWDNKTEAV
                     AAMWGRFYNGIVEANRLIEVLNPENLPMIELTPAGAKNLAKLKIMRSFYYYLLIDNYG
                     DVPYVTSFKNAEKQPNKTPRADVFNNIVAEIEESIPYLDESMSKTSVSKGMAWSLLAK
                     LYLNAEVYTGTPQWVKAENACDSVLALGYSLETDPLAPFVTNNQNSLENIFTIPYDED
                     TYQGFNLHMRTLHYNSNLTFDMSVGPWNGFAVTEQHYNTYPQNDKRKDGYFLVGQQYT
                     STGQPIVDAVAGKPLVFNPYIPKLVMDASFTPEEIRMSGARVKKFEIKLGAKENLSND
                     FPIFRLADIMLMKAEAMVRQSKNGDDYINPIRRRAGLADLNNATLDDILAERGREMFW
                     EAHRRQDLIRFGKFNHAWWEKPASDVSRRIFPIPQWAIDSNPNLAK"
     gene            6287..7759
                     /locus_tag="PK839_09075"
     CDS             6287..7759
                     /locus_tag="PK839_09075"
                     /inference="COORDINATES: protein motif:HMM:NF027726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:2001070 - starch binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusF/SusE family outer membrane protein"
                     /protein_id="HOG72926.1"
                     /translation="MRTTKFRLIWLLAAAALAFTACDNDDDDDEQPPILVEDGLYIVG
                     EATAWTDYDSKGQMAGGINEVDQQPRSTMFEKYVALEAGNTFKIVEIAGKQVIEYGPD
                     NLVDVETNGENDQPNVTAKVGSYKTGGSYTVNESGLYHVVIDKELQKIAIIPVPYWGI
                     LGGSTPYGWDDAGTKLELDGSFDKQQMEFKITNLELRKGDFKFRYGSGWKFQLNDEGT
                     VKVNTNFGGAVNELVPGGANILLTVDEEGIYTVTMKWSAETGGLGMTASMLKTADVEP
                     LPEYPETLFMIGDGVGDWVWDNTDLPMVPVHSHPYMFWKIVWMNETGGFKFAPKKVWA
                     NDFGKVGDMSADSVYSIGSDNVPVLGNAGYYMVVVDLKENKISIEDVKVHLIGSTIGN
                     NWEAPVADGKFTVDNANEVVTITKTLSAGELRMHVWHKWFPIQTPQPVDWWQAEFIIL
                     NNKIEFRGTGGDQERVNLTTEGNYKIDLNFKTGAGSITPQ"
     gene            7817..10609
                     /locus_tag="PK839_09080"
     CDS             7817..10609
                     /locus_tag="PK839_09080"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOG72927.1"
                     /translation="MKKIVSFLGIGFIAFGLKAQPITVTPALPTDADAVTIVFDATKA
                     SRTDLVGYTGDVYAHTGVAINGRKWQYVIGSWGNNATQPKLTRTGTNTYTLSISPSIQ
                     EFYSVPADSAITQMCFVFRSADGTKQTEDIFYDVYEVGLSVSITAPTLLTPIYELNDN
                     VQITASANNSTSLTIFIDNVEMATTTETSLTYTYPATEYGKHWIKAVASNNDQSVADS
                     AYILVRSPLVTEELPAGMKPGVNVTSEQAATVVLYDPQALKNYAYLIGSFSDWLPDEQ
                     YYMKRTPDGKYFWVELNNLNPNQEYAFQFLIDGYLRIADPFTHKTLDPNDQYIPSSTY
                     PGLMSYPTDKTYGIASVFSTAANTYTWDASDFTPPAKNNLIIYEMLIRDFVGDSYIQT
                     AMDSLSYLKKLGVNAVELMPISEFEGNDSWGYNPSFYFAPDKAYGKANDYKAFIDEAH
                     RQGMSVIMDIVLNHSFGQSPLVQLYSNSNGVTLGVPTTDNPWYNQTCPHEPFCWGYDF
                     NHESPETRKFVDSVLTYWLTEYRVDGFRFDFSKGFTNTPNAGSAYDQARIDNLKRIAD
                     KVWSVNPNAYVILEHFCDNSEEKTLAEYRNAEGKGMLVWGNLTYNYGEATMGWLNNSN
                     FSAVAYTQRGWSVPNLVGYMESHDEERLMYKNLTYGNSSNPSHNVKQLPVALKRMELA
                     ANFFIPIPGPKMIWQFGELGYDVSIDYNGRLGRKPPHWEYYAQTERRNLYNIFANLNR
                     LKQMFDVFNTSSYEYMLSGAKKWIKLDGADMDVVVMGNFDVNGVSFAIDFPTTGKWYE
                     YYTGDSIELSATNTSFSMAPSEYRLYTSKKVNRDDIFIGVVENPDKTSESVRLWPNPS
                     NGEFQLSFTLPKAAEVQLELFNLMGQKVYHMRQQGILGTNRLEVDLPPEIVTGLYIFR
                     INSQFYNQTGKLSIVK"
     gene            10694..11803
                     /locus_tag="PK839_09085"
     CDS             10694..11803
                     /locus_tag="PK839_09085"
                     /inference="COORDINATES: protein motif:HMM:NF020965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HOG72928.1"
                     /translation="MASCKIQTIILLVGMMPLGASVFAQPDSTFGSNPRRSTFETCLI
                     LGKIIPHDDTLNQLVNRNIKATEIQYSFKKSGRVIPYHQGLSLVIADLGNHDHLGYAF
                     GVIPNVSITTNTKNRVMVNLRLGLGAAWVTKKFSFASNIENVAISTNLNIMASLNANL
                     QIRITEKSKIYLGLNALHLSNGGFKKPNYGLNMLGASGGFSIAFSNKRECKVPKKSSD
                     DLQIETWPQIKLYVGMGVKETGDAGGPKYFPVSLQIAAARRVAENVALGFGVDIAHDR
                     SSKFQIEQSNEVYNSPNDDFQVGASIYAEVPLRRFSVFARQGFYLYNPNPTLPVGYQN
                     IGFSYMVGHRSAIFISLKSHYDKADHIYAGLALSI"
BASE COUNT         3590 a         2510 c         2509 g         3291 t
ORIGIN      
        1 tgcccaagtt ccttgtaaat actatcgggc gatgaatcga aaagccatgc acctatgtag
       61 agcgcatcgt catcgtaaag tatctttacc ctggttggtt ggcttggcga tgcaccatta
      121 tatggctcgt actgggtaaa gctttccgca aaacctgcaa gctgccattc aggttcatcc
      181 aaatgaccat tgatgtctaa ctgcgtttcg atacgaaatg cttctaccga tttcctgaca
      241 gaaattgtat cgctacttcc atgtgcaagg gtaaaactcg atgaaactat gattgaggag
      301 attgcaaaaa gagcgccccg tgtaaaattc atacctgctc ttgttgttag ttagaatatt
      361 tacccttttg agtgtaatgg gcgaaagggg tttaatggtg aaataaaaaa actatgcact
      421 accagaaggt aaatgttctc tttttaaata aaagcaaagg aataggggac aacaatagga
      481 gttttgctaa ccctgtgcta ccgtaatctg gacgactcgc gtacaatgag ttgggtttta
      541 agaatttcgg taattggagg ctcctggcgg gtttgggtaa ttcttcgaat aagctgctcc
      601 atggcttttt tgcccatttc aaacccaggc tggttaacag tggtaagggt tggctctacc
      661 ataagggatg tttggtcatc gccaaaacca acaaccgaaa catcctgagg aattttaata
      721 ccattgcgga taagaatctt catagcacca gcagccgtaa aatcgtttac cgtaaaaatg
      781 gaatcgaact tagtcccact atcgattagc ttctgtgttt caatcaatgc agactggaat
      841 gtatcacaat ggataatcaa ccgctcatca attaccacgc cgttatccct catggctttg
      901 aggtagccgt taagcctgtt ctggccaatc agcctatttt gaggccccga aaagtgaact
      961 attttccttc gaccctggat aatgagatgc tcggtagcct ggtatgctcc gtcaaaatcg
     1021 tcaataatta ttcggtcggc tgaaatttcc tcaataatcc tatcaaaaaa aaccagggga
     1081 attccctcgt cctcaaatct ctgaaaatgc tcgtaattct cggtttcctt tgaaaccgaa
     1141 acaataagcc cgtcaataat tccgttcaaa aatgcatcgc aggcagcaac ctcacgcttg
     1201 taatcctcac ctgactggtt gaccataacg ctataaccat gggcatgagc aacttcttca
     1261 atgccgctta ttacggtgga gaaaaagtag tgggcaatct caggaataac caaaccaata
     1321 acgttgctac gctggttgcg caggctgagg gcaataatgt ttggcttata gttgtacttg
     1381 gcggccagct cattaaccac tcttttggtt tcctggctta tatcgggatg atcctttagg
     1441 gcacgcgaaa cggtagatgg gctgatgccc agctcgcgtg caatgtcctt aatggtgatc
     1501 tggcttgatt tcattttttt agcaattgaa ctgattgata tatagactgt taataaagca
     1561 tagttatgat tacatcctta acaaatatac tactaaaatt gagctgtaaa agctactcgc
     1621 ccaaggtaat atttttttta tcaaatacaa acgtttgcgg tatcgtttgc gtaaaaaaca
     1681 aagctaaaat caatgttttt atgagattta aatgttaaaa aaatgttaat tgtgaaagtt
     1741 ttcatgcaaa tgtttaacat tttaaattca actaaaaata atattaatca ccatgattag
     1801 atttagacag aatttattag ttgtagcagc attgctatta gggagtttat ctttaatggc
     1861 tcaagaaatt actgttactg gcaaggtgac tgatgctacc gatgggactc caatgattgg
     1921 agtagccgtt gttgtaaagg gtacaactac tggtacaatt actaatgttg atggagagta
     1981 caccattaga tgtaaaggta ccgatgttct tgcattttca tttgtgggat acaataccct
     2041 agaaattgaa gtagctaata ggaatattgt taatgtgcaa atgaaacagg aaacaaagga
     2101 aattgatgag gttattgtta ttggatatgg tgtacaaaag aaaagcgacc gcactggttc
     2161 ggttgtgaat gttaaagccg aagagatgaa taggggatcg cttaccgatc ccatacaagc
     2221 aatgcaaggg aaagttgctg gggtaaacat ttcgaaaaaa ggaggagacc ccaattctgg
     2281 atttgcagtt caaattcgtg gtgcttcagg ttttgcctct aatacccagc ctctttacgt
     2341 tatagatggg gttccaggtg ctgacccaac tacaatttct cctgatgata ttgaatcgtt
     2401 caacgtcctc aaggatgctt catcaggtgc tatttatggt tcaagggcat cgaatggggt
     2461 tattataatt accacaaaga aagccgataa gtctaaagga gctatcattg attacaatgg
     2521 ctatgtatcg gccgatcaaa ccagcaagcg tatgaaattc ctttcggccg atgacattcg
     2581 taaatatgtt accgataaca atatacctga ttttgctgac aatggtgcaa acaccaactg
     2641 gcaggatgaa atttttaaaa caggtatttc acaatcccat tcaatagccg tttcaaattc
     2701 taccgacaat ctttcatatc gcttctctta caatcatacc tacaacgatg gtgtagttat
     2761 tcaaagtaac aagcaacgtg atattgctcg attcaactta acccaaaagg ctttaaacga
     2821 tatcgttaca attgatgcat caattgctgg aactttcgaa aagaataact atgtgaacta
     2881 tggaggaggt atgggttcat ccaatatttt ctaccaaatg taccagcgca acccggtaga
     2941 tcccgtttat aaggatggta aattctttga gtttcagcga gactttaact acaataaccc
     3001 agttgctatt gctaaattga ttcaaaacga acgtgatgcc aaaaactact ttggtaacgc
     3061 taaaatctca gtggaaccta ttaaaggact ggttggtagc gttaactttg gctatataag
     3121 aaacgactcg gaatcgttct actttgagcc aacaaaccta tacgctgggg gaaccgatgg
     3181 ctatggaaaa agagggtatt caaatttcag ctcccaagtt ttagaaacaa ccatttcata
     3241 caacatcgtt cttgaagacc attcaatcaa tcttctcggt ggttactcat ttcaagagga
     3301 taataattct ggactttcag ctcaaggtcg cgaagccctt tccaactacc taaaatcaaa
     3361 caatcttgga catcttaaca acgtaaatgt tggcgatatt ggttcgtggc gtaattcaaa
     3421 ccgattaatt tcatttttcg ctcgcgccac ctataactat caatccaaat attttgccac
     3481 agcaactatt cgtcgcgatg ggtcttcaaa gtttggtaaa aataatgagt ggggtttatt
     3541 tccatcggtt tcaatagcat gggacatcaa gaaggaagca tttctggaaa atatcgacct
     3601 atttaatcag ttaaaactca gggtgggata cggtataaca ggtaaccagg aaatatcatc
     3661 gtttctggat ataatgtatg cttccccttc gggaactgcc cctaattttg aaactggcga
     3721 ggatgccatt aatttcgcta tatcacacaa tgcaaatccc gatttaaagt gggaagagaa
     3781 ttcagaactg aatattgggc tagattactc tatcctgaaa aatagaattc agggttcgtt
     3841 tgagtactat attaaatcga cctatgacct acttgcacca tacgctgttc cagtacctcc
     3901 caacgcgctt ccaaccactt gggcaaacgt aggtcaaatt gataataaag gttttgaagc
     3961 caatatcact ggatatgcca ttgataataa atattttaaa tggcaaacaa ttgttaacct
     4021 ctctactaat caccaaaaac tggttagcct ttcaaatgat cagtactctt ggtccgaagc
     4081 cgataaaaaa cgcctttggt taagtggtcg cgggttagtt ggcgctcaaa actggactca
     4141 ataccttttc gagggtgaag aaataggcac attttatata ccaaaatatg caggactgtc
     4201 aaatgacgga aagttcctat tctacactgc tgcagggggt gttacacgcg atgtaaacct
     4261 ggcacaacgt tatgtggcag gatacgctca gccacgattt acacttggct ggtcaaactt
     4321 atttacatac aaaaactttg atttcagcat tgcactgcga ggagctttcg gaaataaagt
     4381 tatgaatgtt acccatatgg ttttctccaa cccacaaata ttacctaccc tcaatgcgct
     4441 ttccgaagta ctcgatgaaa ttgatagggg tttaaccgat tcgcctaaag taagcgatta
     4501 ttaccttgaa gatgcctctt acattaaagt agataacatc tccattgcat acaactttaa
     4561 tcccaaatct actaagtggg ttaaaaatat gagggtttac cttacatcta acaaccttta
     4621 cacatttact aaatatactg ggctcgatcc tgaacaaaat tatgtaggtc ttgagtacgg
     4681 tttagacatg tatgatgttt atccaaaaac tcgaacggtc acacttggtt tagatattaa
     4741 gttttaacga ataaaaaaat attaaaatga aaagatattt tattttaacg gtaacagcat
     4801 taatggcttt gaccagttgt accgatatag atgaccaatt gacggataaa attccgtttg
     4861 accagtttcc tgaaaacgat gctcaatcgg cattaatggt ggttcctgtt taccgaccaa
     4921 tgcaagactt tctggactgg agtggatggt ggttttgcca ggaagtaacc tccgatgaaa
     4981 tggtttgccc tacccgacat accgactggg atgatggtgg taaatggcgt gttctatatc
     5041 agcatacatg ggataataaa accgaagctg ttgctgcaat gtggggaagg ttttataatg
     5101 ggatagttga agcaaaccga cttattgagg ttcttaaccc cgaaaatctt cccatgattg
     5161 agcttacccc agctggtgct aaaaacttag caaaacttaa gatcatgcgt tccttttatt
     5221 actacctgct gatagataat tatggcgatg ttccctatgt tacctcattc aaaaatgctg
     5281 aaaaacaacc taataaaact cctagggcag atgtatttaa taatatagtg gctgagattg
     5341 aagagtctat tccctacctt gacgaaagta tgtcaaaaac atcggtttcg aaagggatgg
     5401 catggtcact tttagctaaa ctttacctga atgctgaggt ttacacaggt actccccaat
     5461 gggttaaagc agaaaatgct tgcgatagtg ttcttgcgct tggctactca ttagaaacag
     5521 accctcttgc cccatttgta actaacaatc agaattcact agaaaatatc tttaccatac
     5581 cctatgatga ggatacatat caaggattta atttacacat gcgaacccta cactacaaca
     5641 gcaacttaac atttgatatg agcgtaggac catggaatgg ttttgcggtt accgagcagc
     5701 attataacac ttacccgcaa aacgataagc gtaaagatgg atatttttta gttggacaac
     5761 aatacacttc aacgggtcag cccattgttg atgccgttgc cggaaaacca ttagttttta
     5821 acccttatat acccaagtta gtaatggatg cctcttttac ccctgaagaa ataagaatgt
     5881 ccggtgcaag ggttaagaaa tttgaaatta agctgggtgc taaagaaaac ctgagcaacg
     5941 atttccccat attccgcctt gccgacataa tgctcatgaa agctgaggct atggttcgcc
     6001 agagcaaaaa tggggatgat tatattaacc ccattcgccg tagggcagga ctagccgatc
     6061 ttaacaatgc aacacttgac gatatacttg ctgaacgtgg tcgtgaaatg ttttgggaag
     6121 cacatcgcag gcaagacctt atccgctttg gcaagtttaa ccatgcatgg tgggaaaaac
     6181 cggcttccga tgtaagccgt aggatattcc ccattccaca atgggctatt gactcaaatc
     6241 caaatctcgc aaaataaacc aaatgtttaa ccttaaattt aaaactatga gaacaacaaa
     6301 atttagacta atttggctgt tagccgctgc tgcacttgct tttacggcat gtgacaatga
     6361 tgatgatgat gacgaacagc ctcctatttt ggtagaagat ggactctaca ttgttggtga
     6421 ggcaacagct tggactgact atgattcaaa aggacaaatg gcaggtggta tcaacgaagt
     6481 tgatcagcaa ccaaggtcta ctatgtttga aaaatacgta gcccttgaag ctggtaatac
     6541 ttttaagatt gttgagattg ctggaaaaca agtcattgag tatggtcccg ataatcttgt
     6601 cgatgttgaa actaatggag aaaatgatca acccaatgtt actgcgaaag ttggttctta
     6661 taaaacaggt ggtagctata ctgttaatga aagtggtttg tatcatgttg tgattgacaa
     6721 ggaactgcaa aaaatagcta ttatacccgt accttattgg ggcattttag gcggatcaac
     6781 tccgtatggt tgggacgatg ctggtactaa acttgaactt gatggtagtt tcgacaaaca
     6841 acaaatggag tttaaaatta ctaaccttga actccgtaaa ggcgatttca aattccgtta
     6901 tggaagtggt tggaaatttc agttaaatga cgaaggtaca gtaaaagtta acacaaactt
     6961 tggtggcgct gttaatgaac ttgttcctgg tggagctaac atccttttaa ctgttgatga
     7021 ggaaggtatt tatactgtaa ccatgaagtg gtcggctgaa actggtggtt taggtatgac
     7081 tgcaagcatg ttaaaaaccg ctgatgttga acctctacca gaataccctg aaaccctttt
     7141 catgattgga gatggtgtag gcgattgggt ttgggataac actgatctac caatggtacc
     7201 cgtacatagc catccttaca tgttttggaa aatagtatgg atgaatgaaa ccggtggatt
     7261 taagtttgct ccaaagaaag tgtgggctaa tgattttggc aaagttggag acatgagcgc
     7321 tgattcagta tatagcatag gaagcgataa tgttcccgtt cttggtaatg ctggatacta
     7381 catggtagtt gttgacttga aggaaaataa aatttctatt gaagatgtca aagttcactt
     7441 gattggtagt acaattggta ataattggga ggcacctgta gctgatggta aatttactgt
     7501 tgataatgcc aatgaggttg ttactataac caaaaccctt tcagcaggtg aacttagaat
     7561 gcatgtatgg cacaaatggt tccctataca aactccgcag ccagttgatt ggtggcaagc
     7621 agaattcatc attcttaaca acaagattga attccgtgga acaggtgggg atcaagaaag
     7681 ggtaaatctg acgacagaag gaaattataa aatagaccta aacttcaaaa ctggtgcagg
     7741 ttctattact cctcaataaa taatttaata taaaaagggc gcattttttg cgcccttttt
     7801 ttaataattc aataaaatga aaaaaatagt ttctttttta ggtattggtt ttattgcttt
     7861 tggcctcaag gcccaaccca ttaccgttac accggctctt cctaccgatg ccgatgcggt
     7921 aactattgtt tttgacgcca caaaggcaag tcgcaccgac ctggttggct atactggcga
     7981 tgtttatgct cacaccggtg ttgcgattaa cggaagaaaa tggcagtatg tcattggcag
     8041 ctggggtaac aacgccaccc aacccaagct aacccggacg ggaacaaata cctatactct
     8101 tagcatatca cccagcattc aggagttcta cagtgttccc gccgattcag caataaccca
     8161 gatgtgcttt gtgttccgta gtgccgatgg cacaaagcag accgaggata ttttttatga
     8221 tgtgtacgaa gtaggcctat cggttagcat aacggccccc accctactta cccccattta
     8281 cgagcttaac gacaacgttc aaattacagc ctctgccaat aattctacca gccttacaat
     8341 cttcattgac aacgtggaga tggccaccac taccgaaacc tctctcacct acacgtatcc
     8401 agccacagag tatggtaagc actggattaa agctgtagcc agcaataatg accaatcggt
     8461 tgccgattcg gcttacattc tggttcgttc accgctggtt acagaggaac tcccagctgg
     8521 tatgaagcct ggggttaacg ttaccagcga gcaagctgcc actgttgtgc tatacgatcc
     8581 acaggcgttg aaaaattacg cataccttat tggctccttt tccgactggc tccccgatga
     8641 gcagtactac atgaagcgaa cacccgacgg aaagtacttc tgggttgaac tgaataacct
     8701 caaccctaac caggagtatg cttttcaatt ccttatagat ggctacctgc gaattgccga
     8761 tccctttacc cacaaaaccc ttgaccccaa cgaccagtac atcccctcct ctacctaccc
     8821 tgggctgatg agctacccca ccgataaaac ctacggaatt gcttcggttt tcagcacagc
     8881 agctaatacc tatacctggg atgcttcaga ttttacccct cctgcaaaaa acaacctgat
     8941 tatctatgaa atgctcatca gagattttgt gggcgatagc tacattcaaa cggccatgga
     9001 ctcactcagc tacctgaaaa aactcggggt taacgccgtt gagctaatgc ccatcagcga
     9061 atttgagggc aacgacagct ggggctataa cccctcgttc tactttgccc cagataaggc
     9121 ctacggcaaa gcaaacgact acaaggcctt tattgacgaa gcccaccggc aaggcatgtc
     9181 agtaattatg gatattgtcc taaaccactc ctttggccaa tcgcctctgg ttcaacttta
     9241 ctccaactct aacggcgtta cacttggtgt acctacaacc gataacccgt ggtacaacca
     9301 aacctgccca cacgaacctt tctgctgggg ctacgatttt aaccatgaaa gtcccgaaac
     9361 acgaaaattc gttgactcgg ttctcaccta ctggttaacc gagtacaggg ttgacggatt
     9421 ccgatttgat ttctcaaagg gtttcaccaa cacgcctaac gcaggctcgg catacgacca
     9481 ggcccgaatt gacaacctga agcgtattgc cgacaaggta tggtcggtta accccaatgc
     9541 ttacgtcata ctcgagcatt tttgcgacaa ctccgaggag aaaacgctgg ccgagtaccg
     9601 gaacgctgaa ggtaagggaa tgcttgtgtg gggtaaccta acatacaact atggtgaggc
     9661 caccatgggc tggctcaata actccaactt ttcagcagtt gcttacaccc agaggggatg
     9721 gagtgttccc aacctggtgg gctacatgga aagccacgat gaggaaaggc tgatgtacaa
     9781 aaatttaacc tacggtaaca gcagcaaccc aagccataat gttaagcagc ttccggttgc
     9841 cctgaaacgt atggagctcg cagcaaactt ctttatcccc atccctggcc caaagatgat
     9901 ttggcagttc ggcgagttag gctatgatgt ctctattgac tataatggtc gtttaggccg
     9961 caagccccct cactgggagt actacgccca gactgaacga cgtaacctct acaacatttt
    10021 cgccaacctg aacaggctga agcagatgtt cgatgtattt aacaccagta gttacgagta
    10081 catgctctcc ggcgcaaaaa aatggattaa actggatggt gccgatatgg acgttgttgt
    10141 gatgggtaac ttcgatgtga atggggttag cttcgccatt gattttccaa caaccggaaa
    10201 atggtatgag tactacaccg gtgactccat tgaactttcc gcaacaaata cctctttcag
    10261 catggcaccc tcggagtaca ggctttacac ctccaaaaag gttaaccgcg atgacatatt
    10321 tattggtgtg gtggaaaacc cggataaaac ctcggagagt gttcgattgt ggcctaatcc
    10381 atcaaatgga gaatttcaac tctcattcac cctacccaaa gcggcagaag ttcagctgga
    10441 actattcaac ctgatgggtc aaaaggttta ccatatgcga cagcagggaa tcctggggac
    10501 caataggctg gaggttgatt taccgccaga aattgttaca ggcctataca ttttccgtat
    10561 caacagccaa ttctataacc aaacaggaaa actttcaatc gtcaaatagg attcttttta
    10621 ggtttaggtt tacgaggtgc aatgccaaac ccgtccgtta aaaatattcg gacgggttta
    10681 ctatttttga ggtatggcaa gctgcaaaat acaaaccatt attctacttg ttgggatgat
    10741 gccccttggg gcaagcgtat tcgcccagcc cgacagcact ttcggcagca acccaagaag
    10801 aagcactttt gaaacatgcc tcatacttgg gaaaatcatt cctcatgatg atacccttaa
    10861 ccagctggta aatagaaata taaaagcaac agagatacag tatagcttta aaaagtcagg
    10921 aagggtaatc ccctaccacc agggattgtc gctggttatt gccgaccttg gtaaccatga
    10981 tcacctgggc tacgcctttg gcgtaatacc caatgtttcc ataaccacga acacaaaaaa
    11041 tcgggtgatg gttaaccttc gtttgggcct gggtgctgca tgggtaacaa aaaagttcag
    11101 ctttgcatcg aatattgaaa acgttgccat atccacaaac cttaacatta tggccagcct
    11161 gaatgccaac ctgcaaatca ggataacaga aaaatcgaaa atttatctcg gactaaatgc
    11221 tcttcacctg tcaaatggcg ggttcaaaaa gccaaactac gggttgaaca tgcttggggc
    11281 aagcggtggt tttagcatag ctttcagtaa caagagggag tgtaaagtcc cgaaaaaaag
    11341 ttctgatgat ttgcaaatag aaacctggcc tcaaattaag ctttatgttg gcatgggagt
    11401 aaaggaaaca ggcgatgccg gtggacctaa gtactttcca gtttcgctac aaatagctgc
    11461 agcacgtaga gtagccgaaa atgttgccct cggttttggg gttgatatag cacacgaccg
    11521 ctcatcgaag tttcaaattg agcaatcgaa tgaagtttac aactcgccca acgatgactt
    11581 tcaagtgggg gcaagtattt atgcggaggt gcccctacgc aggttttcgg tttttgcccg
    11641 gcagggtttc tacctctata accccaaccc aacgctacca gtcgggtatc aaaatatagg
    11701 atttagctac atggttggcc atcgttcagc catttttata tcactaaaaa gccactatga
    11761 caaagccgac cacatctatg cagggttagc cttaagcatc tgaaaggtat ttgcccagcc
    11821 ggactagaca gcaggctatt ttttctcgaa taacaagggg aatgccagat tttcggcagt
    11881 gctgagtatt gctttaacag
//
LOCUS       DAOSHM010000069        11371 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_4818_length_11371_cov_22.499470, whole genome shotgun
            sequence.
ACCESSION   DAOSHM010000069 DAOSHM010000000
VERSION     DAOSHM010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 11371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11371
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4818_length_11371_cov_22.499470"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="PK839_09090"
     CDS             complement(<1..255)
                     /locus_tag="PK839_09090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72929.1"
                     /translation="MKRVELDFLFILLFIPVLLPAQVTTDDYMRAEKFLQANLGKHIY
                     NSWVEPSWQKDGCTFVYSVNTRKGKEFFRVDAQARTKVPAF"
     gene            complement(305..2668)
                     /locus_tag="PK839_09095"
     CDS             complement(305..2668)
                     /locus_tag="PK839_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HOG72930.1"
                     /translation="MNQRIKTLREQSLKAVNTLSAERALLITKYYSSQQVEGCPTPIL
                     RAQAFDYLMSHKSICINPGELIVGERGPAPKAAPTYPEVCLHSLDDLQLISTRPKVSF
                     RVDEETFRVYRDTIIPFWRGKTLRDMIFSSVSEEWRKAYDAGVFTEFQEQRAPGHTVA
                     GKKVFQKGMLDLIDDVDASLNKLTKDNNGCTKAKIEELEAMRIAANAIIKFANRYADE
                     LERLAKIEVDANRRVELGEMAAICRRVPAHAPQTFHEALQHYWFIHLGVITELNPWDS
                     FNPGRLDQHLYPFYLNDIKEGKLTHEGAVELLQAFWVKFNNHPSPPKMGVTAEESNTY
                     TDFTLVNLGGVKEDGSDAVNELTYTILDVIEEMRILQPSSMVQVSKKNPDRFVKRAVK
                     IVKTGFGQPSFFNSDAIVQELLSQGKSLTDARNGGASGCVETGAFGTEAYILTGYFNL
                     NKVLELTLNNGVDPLTGKEIGIRTGNAASFKTFNQLYEAFERQLGYFSDVKLEGNLVI
                     ERIWAEEMPVPFLSLLIDDCIANATDYNAGGARYNTSYVQGVGLGSITDNLSALSRLV
                     FDEEALSMDELLKAMNANFEGYDDLRYRLIYDMPKWGNNNDYADKLAVRVFNSFYKAI
                     TGRKTFRGGVFRINMLPTTSHVYFGSKIGAMPDGRLAHEPLSEGISPVQGADRNGPTA
                     VILSAAKIDQIKTGGTLLNQKFSPSFFTTDESLDKMVSLIRSYFKLDGHHIQFNVVSA
                     DTLRDAQKHPEKYQDLIVRVAGYSDYFNDLGEALQNEIIRRTEHEAV"
     gene            complement(2710..3513)
                     /locus_tag="PK839_09100"
     CDS             complement(2710..3513)
                     /locus_tag="PK839_09100"
                     /inference="COORDINATES: protein motif:HMM:TIGR02494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HOG72931.1"
                     /translation="MIATKGLVFDIRHFSVHDGPGIRTAVFLKGCPLRCMWCHNPESQ
                     SLLPENMKRSRKVGEKSFDFDEMVGREMTPNEVVAELQKDIPIYDESGGGATFSGGEP
                     MIQFDFLYQVLEECRRMEIKTAIDTCGHAPLEQFRAVEPLTNLFLYDLKLIDSTQHKE
                     FTGVDNSLILQNLEMLLSRKARIHIRIPLIPGITDTDENLLGIKDFLLSYSGAERIDL
                     LPYHDIARSKYVRLGRDNPFKLSGAYDSQKLSRIQDLFIDLNIPVTVGG"
     gene            complement(3942..4151)
                     /locus_tag="PK839_09105"
     CDS             complement(3942..4151)
                     /locus_tag="PK839_09105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72932.1"
                     /translation="MGLDIKLPIGLMFTIFGIILTVYGIFTNSDPQMYQKSFLVNINL
                     WSGIGMLIFGIFMLALTKKKKSEKK"
     gene            complement(4172..5902)
                     /locus_tag="PK839_09110"
     CDS             complement(4172..5902)
                     /locus_tag="PK839_09110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HOG72933.1"
                     /translation="MEMSFDLKWIDYLVMIIYFVFVLGIGWALKKFMKDANAFLEAGR
                     SMPAWVAGLAFISTNLGALEVMGMTGSGAKYGMLTTHFYWVGAIPAMVFLAMFMMPFY
                     YGSKARSVPEYLKLRYDEKTRGLNAILFALMTVLSSGISMYALAILTEKVLGWNFHVS
                     LLLSAAIVLGYTYLGGLSSAIYNEVLQFFIIVIGLLPLVFLSLHQVGGWEGLQSQLAP
                     VAASHGYEPDAYTTMWRHSGTAGGNPLGVEWYGILLGLGFVLSFGYWCTNFLIVQRAM
                     AAESPRAARNTPLIGAIPKMFIPFLIIVPGMVALALMNNPAAQFTIPTNEAGQPIYDY
                     TIISLLKYYLPSGALGLGITALLASFMSGMAGNISAFNTVWTYDIYQSYIRPATANKE
                     ADDKHYLKVGRLATIFGVLVSIAAAYLASMFGNIMDFLQTIFSMINAPLFAVIFLGMF
                     WKRSTGHAAFTGLLAGFLIALLHHGLTVPAGATTLVKGGWIATLHVYPVEMAQNFWTA
                     IFAFTTSALVTVVISLLTRRTKSNDELVGLVYSLTPRMKEDHVVWYKRSSTLAVVVLL
                     IATILTILFW"
     gene            complement(6562..8715)
                     /locus_tag="PK839_09115"
     CDS             complement(6562..8715)
                     /locus_tag="PK839_09115"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HOG72934.1"
                     /translation="MEIQEMAAQHVLLKSWSYQWKPLFRGYTDKTLYINVGNNEVMEK
                     PVPPEVKEKFIGGKGYDLRLLWDATKPTTKWNDPENEIVISSGPIGGITQYSGTGKSI
                     VCTISPQTDIVIDSNVGGFFGPFLKFSGFDAIEIQGKAKRDVIIYIDGITHKVELFEA
                     PAEPIDSHVLGEVLSEMFANDEADKKNIGIVSTGVAAEHSLFGMLNFTFYDPKRKCIR
                     LKQAGRGGIGTVFRDKKIKAIVCKIPGVKGNLNNVADLEAIQERGRRFNKEIRELDDK
                     QCQMRQKGTAHLVEIMNDYDLLPTHNFQYGSHKEAVKIHSEVWKSYFTQGVPDGCWIG
                     CNMACAKGVDKFKLKTGPYKGQEVIVDGPEYETAAGLGSNGGMFDPEYVIEANFYCDT
                     YGICTITWGTTLAFILECYQRGVLNKERTGGLDLTWGNTSDALELLHQLARGEGFGKI
                     AGLGVHKLKEYFIREFGADPQLMQDIGMENKGLEYSQYVSKESLAQQGGYAMTNKGPQ
                     HDEAWLIFMDMVNNQIPTFEAKAEALHYFPMFRTWFGLQGLCKLPWNDVEPANNAETD
                     EPAKVPEHVDNYVTIYNAVTGSKIDKHEIIRQSERVYNFQRIFNIRRGYGLRKNDAQP
                     YRAAGPVTREEYLSREERYDKQLKELVGVDPAKMTLEEKMAALRKYRENRYEQLLDAV
                     YKRRGWNKNGVPTIAHLKEIGMDLPELIEVVTPLQ"
     gene            complement(8894..9928)
                     /locus_tag="PK839_09120"
     CDS             complement(8894..9928)
                     /locus_tag="PK839_09120"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF008277.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HOG72935.1"
                     /translation="MNLRICPWGNIHGKEVYLYTATTRNGMKCTISNYGGLIKSIALI
                     SEDNKELEVAYGFDSIESYREADYHPGGIIGRYANRIAGSEFTIDGHNHQLSCNEGSN
                     QLHGGINGFDRKIWVPEIVEARDGVCTIQLFLHSPDGDEGFPGNLDVWLTYSITEDNQ
                     IAISYKAATDKPTHVNLTTHGYFNLSGFRKNVLDHLLHLSADHYLPVDECLIPTGELR
                     NVSHTPFDFRTTKSIGADINRCGGFYDHCFALNNPTLDKPAAVLRNPDNGVCLKLFTT
                     QPGLQLYTPINRPNVSNPAIPFPESGNWAVCLEPQHFPNTPNMPDFPTTLLMPNGEYS
                     QTTIISFEQE"
     gene            complement(9962..10279)
                     /locus_tag="PK839_09125"
     CDS             complement(9962..10279)
                     /locus_tag="PK839_09125"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HOG72936.1"
                     /translation="MSVESRKFWRWFLSKLKNKFFLTAVIFLLWMLIFDSSNWISMWE
                     TKRNIQRLENERDYYIQKIQDDRRKINELQTNNENLEKFAREQYLMKKPNEEIFIVDE
                     EDL"
     gene            complement(10283..10492)
                     /gene="thiS"
                     /locus_tag="PK839_09130"
     CDS             complement(10283..10492)
                     /gene="thiS"
                     /locus_tag="PK839_09130"
                     /inference="COORDINATES: protein motif:HMM:TIGR01683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HOG72937.1"
                     /translation="MDIILNGIPEHIDTTKVNITVDELLRFKKFSFKMLVVRINGELV
                     RKEQYSTATITNGDNVQVIHLISGG"
     gene            complement(10663..11271)
                     /gene="thiF"
                     /locus_tag="PK839_09135"
     CDS             complement(10663..11271)
                     /gene="thiF"
                     /locus_tag="PK839_09135"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS adenylyltransferase
                     ThiF"
                     /protein_id="HOG72938.1"
                     /translation="MPSAREVKEALRGYSVGIAGVGGLGSNVAMLLTRVGVGHLVIAD
                     YDTVTEDNLSRQYFFWHQVGWSKIRALRENLLAINPMVDVRGHELKVDEDSALGVFDG
                     VDVLVEAFDDARMKKVLIEGFQLNRPDVPIVAGSGIAGWGKSNDIRVVQYGNIYLCGD
                     GVSEVSDDLPPLSPRVSICASMQANVVVSILLGRFWGGEPQP"
BASE COUNT         2948 a         2771 c         2518 g         3134 t
ORIGIN      
        1 gaatgccggg acttttgtac gagcttgcgc atcaaccctg aaaaactcct tccctttccg
       61 ggtgttcacg ctatacacaa aagtgcagcc atccttctgc cacgacggtt ccacccagct
      121 gttgtagata tgttttccga ggtttgcctg aagaaacttc tcagcccgca tgtagtcgtc
      181 cgtggttacc tgtgccggta atagtaccgg aatgaatagc agtatgaata agaaatcaag
      241 ttctactctt ttcatgattt acagtgttta ttgattttgg catagcccat caacacgttt
      301 gccgttaaac ggcctcatgc tcggttcggc ggataatttc attctgaagg gcttcgccca
      361 aatcgttgaa gtagtcggag taaccggcaa cccttacaat tagatcctga tacttttcgg
      421 ggtgcttttg agcgtcgcgc agcgtatcgg cgctaaccac gttaaactga atgtggtgac
      481 cgtcgagctt aaagtagctc cgtatgagcg ataccatctt gtcgaggctt tcgtcggtag
      541 tgaaaaacga tggcgaaaac ttttggttaa gcagcgttcc tccggttttg atttggtcaa
      601 tcttagctgc cgaaagaata acagcagttg gtccgtttct gtctgctccc tgcacggggc
      661 taattccctc gctgagaggt tcatgagcca agcgtccatc gggcattgcc ccaattttgc
      721 tgccaaagta aacatggctg gtggtgggga gcatgtttat tcggaaaacc ccgcccctga
      781 aagttttccg tccggtaatg gccttgtaga agctgttgaa aaccctaacg gccaacttgt
      841 cggcataatc gttgttgttg ccccatttgg gcatatcgta aatcagtctg tagcgaaggt
      901 cgtcgtagcc ctcaaagttg gcattcatgg ccttaagcag ctcatccata gataaggctt
      961 cctcatcgaa caccagcctg ctcagagccg acaggttatc ggttatgcta cccagcccaa
     1021 ccccctgcac gtagctggtg ttataccttg ccccaccggc attatagtcg gttgcgttgg
     1081 caatacagtc gtctattagg agcgagagga atggaacagg catttcctct gcccaaatac
     1141 gctcaatgac aaggttcccc tcaagcttca catcggaaaa gtaaccgagc tgcctttcaa
     1201 aagcctcgta gagctgattg aaagttttga atgaagcggc attgccggtt cttattccga
     1261 tttccttacc tgttagcggg tcaacaccat tgttcagggt tagctcgagc accttgttca
     1321 ggttgaagta gcctgttaga atgtaggctt cggttccaaa ggctcctgtt tcaacacaac
     1381 cgcttgctcc gccgtttcga gcgtcggtaa gcgatttgcc ctggcttagc agttcctgca
     1441 caatggcatc ggagttaaaa aacgatggct gtccaaaacc ggtcttaact attttgactg
     1501 cacgcttcac aaacctgtcg gggtttttct tgctaacctg caccatgctg ctgggttgta
     1561 gtattcgcat ctcctcaata acatcaagga tggtgtaggt gagctcgtta accgcatcgg
     1621 agccgtcctc cttaacgcca ccaaggttaa ccagggtaaa atcggtgtag gtgttgcttt
     1681 cctcagctgt tacacccatc tttggcggtg aaggatggtt gttgaacttc acccagaagg
     1741 cctgcagcag ctcaacagca ccttcatggg taagcttgcc ctctttgata tcgttcaggt
     1801 agaaagggta gagatgctgg tcaaggcgtc cggggttgaa ggagtcccaa ggatttaact
     1861 cggtaattac accaagatga ataaaccagt agtgctgcag cgcctcgtga aatgtttgag
     1921 gggcatgggc aggcaccctt cggcaaatgg cggccatttc accaagctct actcgcctgt
     1981 tggcatcaac ttcgattttt gccaaccttt ccagctcatc ggcataacga tttgcaaatt
     2041 ttatgatagc attagcagca atccgcatgg cttccagttc ttctatcttt gcctttgtgc
     2101 agccattgtt atctttggta agcttgttta gtgaagcatc cacatcgtca attaaatcga
     2161 gcattccctt ttggaaaact tttttaccgg caacagtgtg gccgggagct cgctgttcct
     2221 gaaactcggt gaaaacgcct gcatcgtatg ccttacgcca ttcttcacta actgacgaaa
     2281 aaatcatatc gcgcagggtt tttcccctcc agaaagggat tatggtatca cggtaaaccc
     2341 tgaaagtttc ctcgtcaacc ctgaacgata cctttggacg ggtgcttatc agctgcaaat
     2401 cgtctagcga atgcaggcat acctcggggt aggtaggggc agccttaggt gccggtcccc
     2461 gctccccaac aataagctcg ccggggttaa tgcaaatgga cttatggctc atcaggtaat
     2521 caaatgcttg ggctcttaaa ataggggtgg ggcagccctc cacctgctgc gaagagtagt
     2581 attttgttat cagcaaagct cgttcggccg acagggtatt caccgcttta aggctttgct
     2641 cccttagtgt ctttattctt tggttcatac ttgtaacatt taactattta aaataagtca
     2701 atggacaaac tagcctccaa cagtaaccgg gatgtttaaa tcgatgaaca aatcctgtat
     2761 ccggcttagc ttttgcgaat cgtaggcgcc cgaaagctta aatgggttat ccctgccaag
     2821 cctaacgtac ttgctgcgcg caatgtcgtg gtagggtagc aggtcaatac gctccgcacc
     2881 gctataggag aggaggaagt ctttaatacc caacaggttt tcgtcggtat cggtaatgcc
     2941 aggaattagg ggaatacgaa tatgaattct ggccttcctg gataaaagca tttcgagatt
     3001 ttgtaaaatt agcgaattgt caactcctgt aaattcctta tgctgggttg aatcaataag
     3061 ctttaggtcg tagaggaaca ggttggtcaa cggttcaacc gctctgaatt gttccaatgg
     3121 agcatgtcca caggtatcaa tcgctgtttt aatttccatc ctgcggcact cttccaaaac
     3181 ctggtagaga aaatcgaact gaatcattgg ctcgcctccc gaaaaagtag ctcctccacc
     3241 agattcatca taaataggaa tatctttttg cagctcggca acaacctcat tgggggtcat
     3301 ctcgcgtccc accatttcgt cgaagtcaaa gctcttttca cccaccttac ggcttcgctt
     3361 catgttctcg ggtagtaggc tctggctctc ggggttgtgg caccacatgc agcggagcgg
     3421 acaaccctta aggaatacgg ctgttcttat gcccggaccg tcgtgtaccg aaaaatggcg
     3481 aatgtcgaac accaaccctt tagtagcgat catgtcaaaa tattatgtta gaattgtgat
     3541 actataaaga acaatacaga ggataacact atcctttagg gagaatgaat atgggattag
     3601 aaaattttca acaaatccag caggcaaaga ggcctttgcc attattatgg tagagggtgg
     3661 aggtattgga tttgttgtac atgataccgc aacttctaaa cctattttta caaaaatacg
     3721 ctatttcagg atacctgtca atgatatttg tcatttatta aacatttagc ttgaagcaat
     3781 ttaacccacc ggttaatggt tgccaataca aattttaggg cataagtaag ctaccttagt
     3841 aatgccaggc atgttatggt agcttattaa ggtaagaccc aaaaaaagaa gaccgctggt
     3901 gctagaccaa cagcctcctt tgaattagct aaagatgtcc gctacttttt ttcggatttt
     3961 ttctttttag tgagcgccag catgaaaatg ccaaaaatta gcattccaat gcccgaccat
     4021 aggttgatat ttaccagaaa ggatttttgg tacatctggg gatcagaatt cgtgaatatt
     4081 ccatacacgg taagaattat tccaaaaatg gtgaacatca gcccaatagg aagttttata
     4141 tctagtccca tgacacttat tctttaaatg attaccagaa caaaatagta agaatagttg
     4201 caatcagcaa aactacaacg gcaagagttg acgagcgttt gtaccatacc acgtgatcct
     4261 ccttcattct tggagtgagg gagtaaacta gtcctaccag ctcatcgttc gatttggttc
     4321 ttctggttag aagcgaaatt acaacggtaa ccagagcgct agtggtaaag gcaaaaatag
     4381 cagtccagaa attctgagcc atttctaccg gatagacgtg taatgtagct atccagccac
     4441 ctttaaccag ggttgttgca ccggcaggca cggttaatcc atgatgcagc agggctatca
     4501 gaaaacctgc aagcaacccc gtgaaggcag catggccagt tgacctcttc cagaacatac
     4561 ccaagaagat aaccgcaaag agcggagcgt taatcatgga gaagatggtt tgaagaaaat
     4621 ccatgatgtt tccaaacatg ctggccaggt aggctgctgc tatgcttacc agtaccccaa
     4681 aaatggtggc taaacgacca actttaaggt agtgcttgtc gtcagcttcc ttgttagcgg
     4741 tggctggacg gatatagctt tggtaaatat cgtatgtcca aaccgtgttg aaggcggaaa
     4801 tatttcctgc cattcccgac ataaatgaag ccagcagcgc tgtaattcca agtcctaatg
     4861 cccctgaggg taggtagtat ttcaacagcg agataatggt gtaatcgtaa attggctgac
     4921 ctgcctcgtt tgtaggaatg gtaaactgag cagcagggtt attcataagc gccagcgcaa
     4981 ccatgccggg aacgataatc aggaatggta taaacatttt agggatggca cctataaggg
     5041 gagtatttct tgctgccctg ggcgattcgg cagccatggc ccgttgaaca attaggaagt
     5101 tggtgcacca gtacccaaac gaaagcacaa aacccaaccc tagcagtatt ccataccact
     5161 cgaccccaag ggggtttccc ccggctgtcc ccgagtgcct ccacatggtg gtgtaggcat
     5221 caggctcata gccatgactc gctgctacag gtgccagctg ggattgtaaa ccctcccaac
     5281 ctcctacctg gtgcaagctt aaaaatacta gcggtagcag accaatcaca atgataaaaa
     5341 actgcagcac ctcgttgtag atggctgacg acaaacctcc cagataggtg tagcccagca
     5401 caatggctgc cgacaaaagc aggcttacat ggaaattcca ccccaacact ttttcggtta
     5461 aaattgccag cgcatacatg gatatccccg aggatagcac ggtcataagg gcaaacagta
     5521 tggcattcaa cccccttgtt ttttcatcgt agcgcagctt aaggtattcc ggaaccgaac
     5581 gcgcctttga accgtagtaa aaaggcatca tgaacatcgc cagaaatacc atggccggaa
     5641 tggccccaac ccagtaaaaa tgggtggtga gcattccata ctttgcgccg ctaccggtca
     5701 tccccataac ctccaatgcc ccgaggttgg tagatatgaa tgccaaaccg gctacccatg
     5761 ccggcataga gcgtccggcc tcaagaaagg cgttggcatc cttcataaac ttttttaatg
     5821 cccatcctat cccaaggaca aacacaaaat agattatcat taccagataa tctatccact
     5881 taagatcaaa actcatctcc atcgtttcag cagtttttgt tgaacgtcgt gttacgtact
     5941 atttatacct gattatataa gctattcatt tcttgtattt tccctcgggt aaaaaaacca
     6001 catccgatta actaaaaaac agaaagttaa gccaatgcag acggctatcg ctaaattttt
     6061 aaatcaaaat cattacgtca agtacaaatt aagctgtgtt tgcctgtcaa atttagagga
     6121 aatttaaaca aaatttaacc tgaagtataa ttgatgagct gccttggcaa ggcattttaa
     6181 acagcaatag ctgtgtaaac attacacgcc cattctctat catgatgaaa tcctgatatt
     6241 tggacacttc ccggagttga tgcaaggtaa tgccagcagc gcagcccatc cctcggggtg
     6301 tagcccttgc cagaaggtga aaatcctcat catagtcttc agtttttagc tgattaatgg
     6361 cttattaaac tcataagcag agatatgaca gattagctgg agtatggaac aacatttttt
     6421 caaaaattga tattctatct ggttgtattt taaagtgtta acagtagtat ggcaaacgta
     6481 cgggtaataa aaatggggtt aacccgtagg ctaaccccaa aaccaactca cgctaacaac
     6541 ttgtaatgtt tacactgttg actactgcaa gggagtaacc acctcaatca attcgggcag
     6601 atccatgcct atttccttaa gatgggctat agtgggaaca ccgtttttat tccatcccct
     6661 acgtttgtaa acggcatcca gtagttgttc gtagcgattt tcgcggtact ttctaagtgc
     6721 ggccattttt tcttcaagag tcatttttgc ggggtcaacg ccaaccagct ctttgagttg
     6781 cttgtcgtat cgctcctcac gactcaggta ctcttcccgg gtaaccggac cggcagccct
     6841 gtagggttgg gcgtcgtttt tacgaaggcc gtagccccga cggatgttga atatacgttg
     6901 aaagttgtac acccgttcgc tctgccttat tatctcatgc ttatcaattt tactcccggt
     6961 tacggcattg tagatggtaa catagttgtc aacatgctct ggaaccttgg caggttcatc
     7021 cgtttcggca ttattggcag gttcaacgtc attccacggg agcttgcata gcccctgcaa
     7081 tccaaaccag gttctgaaca tcgggaagta gtggagggcc tcggcctttg cctcaaatgt
     7141 ggggatttgg ttgttcacca tatccataaa aatcagccag gcttcatcgt gctggggtcc
     7201 cttatttgtc attgcgtagc cgccctgctg ggctagcgac tccttcgaaa catactggct
     7261 atactccaac cccttgttct ccataccaat atcctgcatc agctgaggat cggcgccaaa
     7321 ctccctgata aagtactcct ttagcttatg aactcccaag ccagcaattt tgccaaaacc
     7381 ctcaccgcgg gctagctggt gaagaagttc cagcgcatcg ctggtgtttc cccatgttaa
     7441 atcgagtcct cctgtacgct ccttattaag aacccctcgc tggtagcatt ccagaataaa
     7501 ggccagcgtg gtgccccagg taatagtgca aatgccatag gtgtcgcagt aaaagtttgc
     7561 ctcaatcacg tactccggat caaacattcc gccgtttgat cccaagccag ctgctgtttc
     7621 gtattcaggc ccgtcaacaa ttacctcctg tcctttatag ggtccggttt ttagcttgaa
     7681 cttatccacg cccttggcac atgccatgtt gcagccaatc cagcacccat cgggaacacc
     7741 ctgggtaaag tagctcttcc aaacctccga gtgaatttta acggcttcct tatgagaacc
     7801 gtactggaag ttatgggtgg gtagcaggtc gtagtcgttc atgatttcta ccaggtgggc
     7861 tgttcccttt tggcgcatct ggcactgctt atcgtccagc tcgcgtattt ccttgttgaa
     7921 ccgcctaccg cgttcctgaa ttgcctccaa atcggccaca ttattaagat ttccctttac
     7981 cccgggaatc ttacaaacta tagcctttat tttcttatcg cgaaataccg tgccaattcc
     8041 tccccttccg gcttgcttaa gcctgataca tttccgcttt gggtcgtaaa aggtaaagtt
     8101 cagcattccg aaaaggctat gttctgcagc aactccggtt gacacaatgc ctatgttctt
     8161 cttgtcggcc tcatcgttgg caaacatttc gcttagcacc tctcccaaca catggctgtc
     8221 tatgggttcg gcaggcgcct caaaaagttc aaccttatgg gttatcccat caatgtaaat
     8281 tatcacatca cgctttgctt taccctgaat ctcaatagca tcaaatcccg aaaatttaag
     8341 gaatgggcca aagaaacctc ccacgttgga gtcaatcaca atatcggttt ggggtgaaat
     8401 ggtgcataca atggattttc ccgttccgga gtactgggta attccaccaa ttggacccga
     8461 ggatataaca atttcgtttt cgggatcatt ccactttgtg gttggcttag tggcatccca
     8521 aagcaagcgc aggtcgtagc ctttaccacc aataaacttt tccttcacct ccgggggaac
     8581 aggtttttcc atcacctcat tgttgcccac gttgatgtaa agtgttttat cggtgtaacc
     8641 cctgaaaagg ggtttccact ggtaactcca gctcttcaga agcacatgct gtgcagccat
     8701 ttcctgaatt tccatactca tcgtttttag tcttgcttgc tactactaaa attcttcagg
     8761 taaaaataca ggcaaattta ccgataaaaa atgattattg tcaatatgaa attatttgca
     8821 tattgagcaa cgatgggact ttcaaaagtt ttacaaggta gaaaaccttc tggttgcaac
     8881 agctcaaaat ggactattcc tgttcaaaag aaattatggt tgtttgcgaa tactctccat
     8941 tgggcataag tagtgttgtt gggaaatcgg gcatattagg ggtgtttgga aaatgctggg
     9001 gctcaaggca gactgcccag tttccacttt cagggaatgg tatagccggg ttggatacat
     9061 ttggcctatt tatgggtgta tagagctgta ggcccggctg cgtggtaaag agcttaaggc
     9121 aaactccatt gtcggggttg cgaagcactg cggccggttt gtcaagcgtg gggttgttga
     9181 gcgcaaagca atgatcataa aagccaccgc agcggtttat gtcggctcca atggatttag
     9241 tcgtcctaaa atcaaagggg gtgtgcgaaa cattgcgaag ctcaccggta gggatgaggc
     9301 actcgtcaac gggtaagtaa tggtcggcac tcagatggag taaatggtca agaacgttct
     9361 ttctaaagcc acttaggtta aaatacccat gggttgtcag gttcacatgc gtgggcttat
     9421 cggtagctgc cttatagctt atggcaattt gattatcctc ggtgatgctg taggttagcc
     9481 aaacatcgag attgccgggg aacccttcat ctccgtcggg gctgtgcagg aatagctgaa
     9541 tggtgcaaac gccgtccctg gcctctacta tctcaggcac ccatatcttc cggtcgaatc
     9601 cgttaattcc gccatgaagc tgattggaac cctcgttgca cgaaagctgg tggttgtgcc
     9661 catctattgt aaattcacta cctgcaatcc tgttggcgta ccgtccaata attccgcccg
     9721 gatggtagtc ggcctccctg tacgattcaa tactgtcgaa cccgtaagcc acctctagct
     9781 ccttgttgtc ctcgcttatt agcgctatgg acttaattag cccgccatag ttcgaaatgg
     9841 tacacttcat gccattgcgc gtagtagcag tgtataaata cacctccttc ccatgaatat
     9901 tgccccatgg acaaatgcgt aaattcataa taaaaagtga ttaggtttga aagtttaagc
     9961 tctaaagatc ttcctcgtca acgataaaaa tttcctcgtt gggctttttc atcaggtact
    10021 gctcgcgggc aaatttttca aggttttcgt tgttggtttg cagctcgttt atttttctcc
    10081 tgtcgtcttg aattttctga atgtagtaat ccctttcatt ttccaacctt tgaatattcc
    10141 gctttgtctc ccacatcgaa atccagttgc tgctgtcgaa aatcagcatc cataatagaa
    10201 aaataacagc ggtaagaaaa aacttatttt taagttttga caaaaaccat cgccagaact
    10261 ttctgctttc taccgacata gcttaacctc cgcttatcaa atgtatcacc tgaacattat
    10321 cgccgttggt aatagttgcg gtggagtatt gttccttccg caccagctca ccgttaatgc
    10381 gcaccaccag cattttaaag ctgaattttt taaagcgaag cagctcatca acagtaatgt
    10441 taaccttggt agtgtcaata tgctcgggta tcccgtttaa aattatatcc atttcctttg
    10501 tttgggcgtg cccccttcgg ggtcgggctg cttcgggctt cggcaatcct cattttggcc
    10561 agggccaaaa tgaggcagtg ctgctacgca cccttcgcat ccctcacgca aaattagtaa
    10621 actttacatg caaataagca atttcgtttc ctgttaggta aactatggct gcggttcacc
    10681 cccccagaag cgtccgagca gaatgcttac caccacgtta gcctgcatgg atgcacaaat
    10741 gctgacccgc ggactcagcg ggggaagatc gtccgaaacc tccgataccc catcaccgca
    10801 caggtaaata tttccgtatt gaaccacccg aatatcgttc gactttcccc atcccgcaat
    10861 tccactgccc gccactatgg gcacatcagg cctgttaagc tgaaaacctt caatcagcac
    10921 tttcttcatc cgggcatcgt caaatgcctc aaccagcaca tcaacaccat caaacacgcc
    10981 aagcgcagag tcctcgtcca cctttaattc atgcccgcga acatctacca tcggattaat
    11041 ggcaagcagg ttttccctca aggcgcggat cttgctccag ccaacctggt gccaaaaaaa
    11101 gtactgtcgg cttaggttgt cttctgttac cgtgtcgtag tcggcaataa ccaaatgacc
    11161 aaccccaacg cgggtgagca gcatggccac attgcttccc aatccgccca caccggctat
    11221 tccaacagaa tagcctctta atgcttcttt tacctctctg gcagatggca ttagattttt
    11281 actttgagca aaattacgtc attttagatt tcattcacat cgggttggtt taatttatct
    11341 ggtgctgaac agttggcggt atgtgcagtt g
//
LOCUS       DAOSHM010000070        11366 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_4822_length_11366_cov_20.359561, whole genome shotgun
            sequence.
ACCESSION   DAOSHM010000070 DAOSHM010000000
VERSION     DAOSHM010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 11366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11366
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4822_length_11366_cov_20.359561"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1199)
                     /locus_tag="PK839_09140"
     CDS             complement(<1..1199)
                     /locus_tag="PK839_09140"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HOG72939.1"
                     /translation="MNIEEYLAGFSLSGSAGIKTQVLDLPVRGSTYPVFINEYWTSKQ
                     RQASSIHEISYRACFKPQLPHFFIERLTSEDETVYDPFLGRGTTIIEAALMGRNVAGN
                     DVNPLSLMLTRPRLFIPNLADVQQRLSSIPVNSSLRASSDLSMFFHPQTEAEIVSLRN
                     YLDTRKQQEKEDEVDQWIRMVATNRLTGHSVNFFSGYTLPPNQAISAEKQRMLNIKHG
                     KTPGYKNVKSIILKKSGDLLRNISEEETSRLRKAGEKAILLNTDARHANTLPDNSVSL
                     TITSPPFLNVVQYAADNWLRCWFNNINIQDVASRITMSKTIEEWENVMREVFVMLYSK
                     TKHKGYVAFEVGEVRNGKVKLENHVVPLGLEAGFDCVGILINQQKFTKTSNIWGVSNN
                     SRGTNTN"
     gene            complement(1386..2363)
                     /locus_tag="PK839_09145"
     CDS             complement(1386..2363)
                     /locus_tag="PK839_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HOG72940.1"
                     /translation="MDQNKPISSFIEKHYLHFNAASLVDAAKAYKKHLADGKKMMITL
                     GGAMSTAELGISLAEMIRQDKVQIITCTGANLEEDLMNLVAHNHYKRVPDYRDFTPQQ
                     EWDLLQSGLNRVTDTCIPEEEAFRRLQSHLFDIWKSALDKGERFFPYEYMYKLIRSGV
                     LKQYYEIDPKNSWMLAAAEKNLPIIVPGWEDSTMGNIFAAYCIKNELKPSIMKTGIEY
                     MMFLADWYTKNAGKEGIGFFQIGGGISGDFPICVVPMLTQDLEREDTPFWSYFCQISD
                     STTSYGSYSGAVPNEKITWGKLGINTPKFIIESDATIVAPLIFAYVLGW"
     gene            complement(2387..2830)
                     /gene="rpiB"
                     /locus_tag="PK839_09150"
     CDS             complement(2387..2830)
                     /gene="rpiB"
                     /locus_tag="PK839_09150"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HOG72941.1"
                     /translation="MEKEKLAIASDHAGYHLKEDLKQFLGELGYHVWDFGTHSEDSVD
                     YPDFAHPLAQAVENGEYRFGFLLCGSGNGVSITANKHQGIRAALCWIPEIASLARMHN
                     DANVCSIPARFVSAQEAKLIAKAFLDARFEGGRHSRRVEKIPLSK"
     gene            2960..4102
                     /gene="hemW"
                     /locus_tag="PK839_09155"
     CDS             2960..4102
                     /gene="hemW"
                     /locus_tag="PK839_09155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HOG72942.1"
                     /translation="MAGIYVHIPFCRRKCLYCDFYSVGERFITGDFDKLINHELLLRG
                     NFLNDKRISTVYLGGGTPSLLPIESVGRILEGIDTLSSLNGDAEITIEVNPDDVSLEL
                     FRSYKRLGVNRISIGVQSFNQAELSFLGRRHSVESSSVAVHNAQDAGFENISIDLIYG
                     IPGSTLQTWEHSLSVAENLNPQHLSCYHLTYEGNTPLTRRLRKGLFTALDEELSLGQF
                     ELLRSFAQSKGYLHYEVSNFAKEGFISRHNSSYWLGEEYLGLGPAAHSYNGAVRQWNP
                     YSLERWEDGIKNNSPNIEMESLSAVSKYNELLITRLRTIWGVNTDDVSRMFGESFASF
                     LNKSAQKYIDDGRMSRTKGNTLLISPSAYFVSDSIVENLMLIAEPD"
     gene            4199..7321
                     /locus_tag="PK839_09160"
     CDS             4199..7321
                     /locus_tag="PK839_09160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOG72943.1"
                     /translation="MKLVILLVFVIIRRNTFRFWIILTVAFIAVTALTIVPLVLAKKH
                     YAELIRREFDANIQEIHNRSTGYFQTIENGIANNLTLETYNDPLVSLKFTTDSYVRVQ
                     NVRREGKTVYSSPSDDNSEFYLFSESKVFPFEVATAVKRSDGFWGMKSFGFESYNVDY
                     LFRFDNLLSYLQGLTACNICMTLGDIQINPRTGSNSAAFRWNPYVLESGKWPAVVAQN
                     KQYFYVVNLAEVVDVSGNKAGMTFLAVDVSEIHRLFVSLVGLVSILGALSILLLGLFA
                     VKRYRFLKTAAKHEDNIKTNEEDLLRRASLVNLFDAMENGVVMINTDFEVVYINESYA
                     DFTGLQHSTTVGEKCYSALQGMYCHTAECPLSRILAGEPRVETTEFRFKSNGEKARFA
                     VATNPITDDSGKILGIIENFKDITEKLEVEQTLKQTEEQFEIFMNSFPLGVFIEDGIT
                     HEIIYQNAYLNDISNGACIKDFLPKEKSVKPVKSYRESTEEVMLVDSSGHWRYFLLHK
                     FKFIGVGNNLRIGGIMIDTTERREVERYRDVLSEAIDNSPVSIVILSPATEIEFVNPY
                     FLELTGYRIEEVYSKDINSLDLEYNSVKVLGKAVAAALGGRVWQGEVHLKHRNIGHSW
                     VSASFIPITDSTGSLQNIVGVMENITQHKEYEKEILLAKSKAEESDNLKSAFLSHLSH
                     EIRTPLNAIIGFSSLLSDSDLSLTERRNLSEIIYRNSNDLLRLIENLIEISEIETGTL
                     DIKKSQFSVNALMHSLYLQILEEDKKATNVRFSLRREVHHDDMVIYSDEAHIRQVLFH
                     LLVNSCKFTNHGFIEFGYSIVDEFTIMFYVLDSGVGIETDKQALIFNPFRQVDESSTR
                     QFGGMGLGLAISKHIVEKLGGKIWFNSTPGSGTNFYFTVPYIPVNSKFEVDNVGSRLQ
                     KFNWSSKTILVADDIDVNFVYLKAALKRTNAQVLWAKNGKEAVEIVRQGPESINLVLM
                     DLVMPDMDGFEATRLIKDFDSSIPVIGQTAYPNPGNAEAVNQYGLDSLLEKPIKINSL
                     LNELNKYLDN"
     gene            complement(7335..8696)
                     /locus_tag="PK839_09165"
     CDS             complement(7335..8696)
                     /locus_tag="PK839_09165"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOG72944.1"
                     /translation="MIPDKLNILLQTLTATPNMVGMAVLLGMLLVIGLLLYRNKQLKN
                     NNKELLATIRQLQTMNARQEEISATLTDKLNDTLQKLSELTECKTSFWVDLNHRMRTS
                     LNGLLGFSQALLMNNTTKKEKDAAAQQIQQLCNKIILLADEMVDLAKIEANQLEVLPA
                     SCNINKLISDILHQLTENREYMSRQVALKLALSLDDEQAIMVTDADKLKKTLLHILGK
                     LSKLAESDTLEIGYRVDSNASITFHVREVKQPFTDTSSNEIILRQSVRSNGKHFTRQQ
                     GEAEIGMIIANGYAKALGGELDFGSTPDAGNVVYLRLPLNISKKKQSNELFCSPENWS
                     GKTILVVEDDLMNFLYIEALLKRTNVRLIHAKNGEDAVEITLLHPEISLILMDLRLPF
                     MDGYRVVREIRKQNHDIPIVATTASSMANEEQRSLDAGCNAFITKPLDSDELYKTIRR
                     YIR"
     gene            complement(9059..9622)
                     /locus_tag="PK839_09170"
     CDS             complement(9059..9622)
                     /locus_tag="PK839_09170"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOG72945.1"
                     /translation="MDADLRRTIDTLKSGGIILYPTDTVWGIGCDATNPAAVDKIFRL
                     KQRDDSKSMIVLVDQPGRIPSYVDEVPEVAWDLVELADKPLTIIFQKAKNLAPNLIAP
                     DGSIGIRVVRHDFCQKLILQFGKPIVSTSANISGRPFPKNFSEVDEVIKSGVDFIVPQ
                     TYDHPVSDKPSGIIALGDGGLVRIIRE"
     gene            complement(9628..10689)
                     /locus_tag="PK839_09175"
     CDS             complement(9628..10689)
                     /locus_tag="PK839_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018108175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047355 - CDP-glycerol
                     glycerophosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HOG72946.1"
                     /translation="MLKAVLFCANPYAFGILKPLHDTLVELHHEVVWYIPERIAPKCP
                     FINEVKVFNSIKEVYVYKSDAIFVPGNEVPNYLKGVKVQVFHGLAGEKKGHFRIRGYF
                     DLYLTQGPYFTKRFTELSNHYGNFEVVETGWCKLDPLYRLASTIGNEKTTILQKHGKQ
                     KIVLYAPTFSPSLTSAPGAIDHIFNLACKLNIYLMVKFHDLMNSETVETYKTKAKIFD
                     NVEVVEDSNIIKYLSLADLMISDTSSAVYEFLLLDKPVVTICSNAETIQWQNILKPED
                     LITVVEQELECSQFAAQRKRVIEAYHPYSDGKSSARMVNAVENFIERNGIPDSRKLNL
                     YRRHKIKKLFGPKPQINKP"
     gene            complement(10713..>11366)
                     /locus_tag="PK839_09180"
     CDS             complement(10713..>11366)
                     /locus_tag="PK839_09180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72947.1"
                     /translation="SRLGNPLPKPLTVLQSGKTIMQQQLDNLTEYFNPDDINIVVGFK
                     KDLIMENFPDISYIYNQNFDVTNTSKSLLKALRKHRNESVLWLNGDVVFDSAILGEIK
                     THIENNNSFVCVNNSAVGEEEVKYNLDEFGFIKNISKKVKHALGEAVGINFISSNDIR
                     AFISRLEECENSDYFERGLELAIEKDKIKVLPHDVSSMRCMEIDFREDLENANRLFK"
BASE COUNT         2934 a         2456 c         2676 g         3300 t
ORIGIN      
        1 ctgttggtgt tagtgcccct tgagttgttg ctaacccccc aaatatttga tgttttagta
       61 aatttctgtt ggttgattag tattcccacg cagtcaaaac cggcttcgag gcctaagggg
      121 actacatggt tctccagctt taccttacca tttctaactt caccaacctc aaatgccacg
      181 tagcccttgt gtttagtttt ggagtaaagc atcacgaata cctcacgcat cacattttcc
      241 cactcctcaa tggttttaga catggtaatt cgggatgcaa catcctgaat attgatgttg
      301 ttaaaccagc atcgaagcca gttgtcggct gcatactgca ccacgttaag gaagggcggg
      361 gaggttatgg taaggctaac cgagttatcg ggcagggtgt tggcatggcg tgcatcggtg
      421 ttcaggagaa tggccttttc ccctgccttg cgcaggcggc tggtttcttc ctcggatata
      481 ttccgcagta aatcccccga ttttttcaga atgattgact taacgttctt gtagccagga
      541 gttttaccat gtttaatgtt cagcattctt tgtttttcgg cacttatggc ctggttgggc
      601 ggtagcgtgt agcccgaaaa gaagttgacc gaatgacccg taagcctgtt ggttgccacc
      661 attcgtatcc actggtccac ctcatcctcc ttttcttgtt gctttcttgt gtccaggtaa
      721 ttacgcaagc tcactatttc tgcttcggtc tgggggtgaa aaaacatact taagtcggaa
      781 cttgctctta aggaagaatt aacgggtatg ctggaaagtc gttgctgcac atcggcaagg
      841 tttgggataa acaacctggg tcgggtgagc ataaggctca acgggtttac atcgttgcct
      901 gcaacgttac ggcccatgag agcagcttca ataatggttg ttccccgccc cagaaatgga
      961 tcgtaaacag tttcgtcctc ggaggtcagg cgttcaatga aaaagtgcgg aagttggggt
     1021 ttaaaacaag cccggtagga gatttcgtgg atggagcttg cctgccgctg ttttgatgtc
     1081 caatactcgt tgataaatac agggtatgtt gagcccctaa ccggtaagtc taacacctgg
     1141 gtttttatgc cggcactgcc ggaaagggag aatcctgcca gatattcttc tatgttcaag
     1201 gtttagctac tttacaactg cacaaattta ggaaggaatg accacaagaa aaagcatggg
     1261 ggtgcatcgg gggaatattt gactttttgg cttgccaggg ttatgttcaa aagcaaggcc
     1321 ggagagttct ccccggccaa gccaacatct attgtattgc tgcgcaatgg agctgagttc
     1381 accttctacc aacccagcac gtaggcaaat attagggggg caacaatagt agcgtccgat
     1441 tcgatgatga attttggggt gttgatgccc agctttcccc aggtgatttt ttcatttgga
     1501 actgctccgg agtatgaccc gtagctggtg gtggaatcgc taatctggca gaagtagctc
     1561 cagaaaggtg tatcctctcg ttccaggtcc tgtgtaagca tgggaaccac gcaaattggg
     1621 aaatcacctg aaattcctcc gccaatttgg aagaagccaa tgccctcttt cccggcattt
     1681 ttggtgtacc aatcggccag gaacatcatg tactctatac cagttttcat gatgctgggt
     1741 ttaagctcgt ttttaataca gtaggcggca aaaatattgc ccatggtaga gtcctcccag
     1801 cctggaacaa taattggcag gtttttttcg gcagcagcaa gcatccatga gtttttggga
     1861 tcaatctcat agtactgctt cagcacaccg ctacgaatta gcttgtacat gtactcatag
     1921 gggaaaaaac gttccccctt gtcgagggcg ctcttccaaa tgtcgaagag gtgtgattgc
     1981 agcctcctga atgcctcctc ctcggggata caggtatctg taaccctgtt aaggccgctt
     2041 tgaagcaaat cccattcctg ttgtggagta aaatcgcggt aatcgggaac tcgtttgtag
     2101 tggttgtggg ccaccaggtt catcaagtct tcctcaaggt ttgccccggt acaggttata
     2161 atctgaacct tatcctgcct tatcatttcg gccaggctta taccaagctc ggcagtgctc
     2221 atggcgccgc caagggtgat catcatcttc ttgccatcgg caaggtgttt tttgtaggcc
     2281 ttagctgcgt caacaagtga agcagcgttg aaatgcaggt aatgcttctc gataaacgag
     2341 gagattggtt tgttctgatc cattttctta gaatattgtt gggttgttat ttggatagtg
     2401 gaattttttc aactctacgg gaatgccggc caccttcaaa ccgggcatcc agaaaggctt
     2461 ttgcaattag cttagcttcc tgagccgaaa caaatcgggc cggaattgag caaacgttgg
     2521 catcgttatg catacgggca aggctggcaa tttcagggat ccagcaaagt gcagccctaa
     2581 ttccctggtg cttgttggca gttatcgata cgccattccc gcttccgcaa agcaagaatc
     2641 caaaacgata ttcgccattc tcgaccgctt gtgccagcgg atgggcaaaa tcgggatagt
     2701 caacgctatc ctcgctgtgc gttccaaaat cccaaacatg atatcccaat tccccaagaa
     2761 actgctttaa atcctctttc aggtggtaac ctgcatggtc ggaggcgatg gctagctttt
     2821 ctttttccat gttgccgggt ataattacct tttctgccaa tagatgaaat ggtttgtgtt
     2881 cgtataacgt taactttgca aagataatgg ctttttggct tttatatctg tttttgtgtc
     2941 gtaatcattt ggtgacaagg tggctggtat atacgtgcat attcccttct gcaggcgcaa
     3001 gtgcctctac tgtgattttt actcggtggg ggaaaggttt attaccggtg attttgacaa
     3061 gcttatcaac catgaactgc tgctacgagg aaacttctta aacgacaagc gcatcagcac
     3121 tgtttacctt ggcgggggaa ctccctccct gcttcccatt gaaagtgtcg ggaggatact
     3181 tgaaggcatt gatactctta gcagcctgaa tggtgatgct gaaatcacca ttgaggtaaa
     3241 cccagatgac gtttcgttgg agctttttcg gagctataag aggctgggtg taaacaggat
     3301 aagtataggc gtgcagtcgt tcaaccaggc agaacttagc tttctggggc gacggcatag
     3361 cgttgaatcc tcctcggttg ctgtgcataa tgctcaggat gcaggttttg aaaacatcag
     3421 catagatttg atatacggca ttcccggctc aacgcttcaa acctgggagc attcactaag
     3481 tgtggcagaa aatttgaatc cccaacacct ttcgtgctac caccttacgt atgaagggaa
     3541 caccccgctt acccgtaggt taaggaaggg attgtttacc gcccttgatg aggagttgag
     3601 ccttgggcag ttcgaactcc ttcgtagttt tgcccaatca aagggctacc tccactacga
     3661 ggtctctaat tttgccaagg aaggatttat ttccaggcac aatagctcct actggcttgg
     3721 tgaggagtac ttaggccttg gaccggcagc ccattcctac aatggagctg taaggcagtg
     3781 gaatccttac tcgctagagc gctgggaaga cggaataaag aataattcgc caaatattga
     3841 aatggagagc cttagtgctg tttcaaagta taatgagctg ctcattacca ggttacgaac
     3901 catttggggt gtgaatacgg atgatgtttc ccgcatgttt ggagaaagtt tcgccagctt
     3961 tttaaacaag tctgcccaaa agtatataga tgacgggagg atgagccgaa ccaaaggcaa
     4021 tacactcctg ataagcccgt cggcctactt tgtaagtgat agcattgtgg agaatttaat
     4081 gttgatagcg gagccagatt agggcttgct ctacgcctgc aacccgaaga ggtggcgaac
     4141 aatcttttcg taaaagtatt aggcagtgat gtggctaatg aaataaatat tcatattttt
     4201 gaagctagta atattgctgg tttttgtgat tattcgtaga aatacattcc gattctggat
     4261 tatacttacg gttgccttta ttgctgtaac cgccctgaca attgtcccgc ttgttttggc
     4321 caaaaagcac tacgcagagc taatacgtag agagtttgac gccaatattc aggaaatcca
     4381 caaccgttct actggttact ttcaaaccat tgagaacggc attgccaata atttaacgct
     4441 tgaaacgtat aatgacccgc tggtatcgct aaaatttaca accgattcct atgttcgggt
     4501 acaaaatgtt aggcgtgaag gcaaaacagt gtatagtagc ccatcagatg acaattcgga
     4561 attctacctg ttttctgaaa gcaaagtatt tccatttgag gttgccacag cggttaagcg
     4621 ttccgatggt ttttggggga tgaagtcgtt cggcttcgag agctacaatg ttgactattt
     4681 attccgattc gacaaccttc tctcatacct gcaggggtta accgcatgca atatctgcat
     4741 gactttagga gatattcaaa tcaacccaag aaccggaagt aattcagctg catttagatg
     4801 gaatccttat gttctggagt ctggcaaatg gcctgctgtt gtagcgcaga ataaacaata
     4861 cttctacgtg gtcaatcttg ctgaagtagt tgatgtatca gggaacaaag ccggaatgac
     4921 atttttagca gttgatgtta gcgaaattca caggcttttc gtgtcgctgg ttggcttggt
     4981 gtcaatattg ggagcgttat ctatcctgct gttagggctg tttgcagtaa aacgataccg
     5041 ttttctaaaa accgcagcca aacacgagga caatatcaaa acaaatgaag aagatttact
     5101 ccgcagggca tcgttggtga accttttcga cgcaatggaa aatggggtgg taatgattaa
     5161 caccgatttt gaggttgttt acattaacga gagctatgct gattttacgg gtttacaaca
     5221 cagcacaacg gtaggggaaa aatgctacag tgcacttcag gggatgtact gtcatactgc
     5281 cgaatgcccg ttgtcgcgaa tactggccgg tgagccaagg gttgaaacaa ccgagttccg
     5341 gtttaagtca aatggcgaaa aggcacgatt tgctgtagcc actaatccaa ttaccgatga
     5401 tagcggcaaa attctaggga ttatcgagaa ttttaaggac atcaccgaaa agttggaggt
     5461 ggaacagacc ctaaagcaaa ccgaggagca gtttgaaatt ttcatgaact cgtttccctt
     5521 aggcgttttt attgaggatg gaattaccca tgaaatcata taccagaatg cctacctgaa
     5581 tgacatatca aatggggcat gcattaagga tttcttgcca aaggaaaaaa gtgtaaaacc
     5641 agttaaatcg tacagggaaa gtaccgagga ggttatgctg gttgattcat cggggcactg
     5701 gcggtatttc cttcttcata agtttaaatt tattggcgtg ggcaacaacc ttcgtattgg
     5761 ggggataatg attgatacaa ctgagaggcg agaggtggag aggtaccgcg atgtgctctc
     5821 ggaagcgatt gacaattcgc ctgtaagcat tgtaatcctt tcgccagcta ccgaaataga
     5881 gtttgtaaac ccatacttcc tggaactaac cggatatagg atagaggagg tttacagcaa
     5941 ggacatcaac agcctcgacc tggagtataa ctctgttaag gtgctcggca aagctgttgc
     6001 agctgcttta ggaggcaggg tatggcaggg cgaagttcac ctcaagcatc gaaatattgg
     6061 ccatagctgg gttagcgctt catttatccc cataaccgat tctacaggca gtctgcaaaa
     6121 tattgttggt gttatggaga atatcaccca gcacaaggag tacgaaaagg aaatactgct
     6181 ggcaaaaagt aaagccgagg aatccgataa ccttaaatcg gcttttcttt cccatctttc
     6241 acatgaaata agaacacccc tgaatgccat catcggtttt tcatccctgc tttccgacag
     6301 cgacctcagc ctaaccgaac gacgaaacct ttcggaaata atctaccgta actccaacga
     6361 tctgctcagg ttgattgaaa atctcattga aatttcagaa atcgaaaccg gaacgcttga
     6421 tattaaaaag tcccagttct cggttaatgc cctcatgcac tcgctgtacc tccagattct
     6481 ggaggaggat aaaaaagcca ccaacgttcg tttcagcctt cgtcgggagg tgcaccacga
     6541 cgatatggtt atatacagcg atgaggcgca tatacgacag gtcctttttc acctgctggt
     6601 caactcctgc aagttcacca accatggttt catcgagttc ggatactcca ttgtggatga
     6661 gtttaccatc atgttctacg tcctggatag cggcgttggg attgaaaccg ataaacaagc
     6721 tctcatcttc aacccctttc gccaggtaga tgagtcgagc acccggcaat tcggtggtat
     6781 gggcttaggc ctggccattt ccaagcatat tgttgaaaag ctgggcggta aaatatggtt
     6841 caacagcacc ccgggatcgg gaacaaactt ttacttcacc gtgccctaca ttccggttaa
     6901 ctccaagttt gaggtggaca acgtgggcag ccgcctgcaa aaattcaact ggagctctaa
     6961 aactattctg gtagccgacg atattgatgt taactttgtt tacctcaagg cagcgcttaa
     7021 aaggacaaac gcccaggttt tatgggcaaa aaacggtaag gaagccgtag aaatagtaag
     7081 acagggtccg gagagcataa acctggtgtt gatggatctc gtaatgcccg atatggatgg
     7141 gtttgaagcc acgcggctta tcaaggattt tgacagcagc attccggtaa tcggacaaac
     7201 ggcatacccc aacccgggga atgctgaggc ggtaaaccag tatggacttg attcgctgct
     7261 cgagaagcca atcaaaatca actccctgct caacgagctc aacaagtatc tggataacta
     7321 accctggttg taagctaccg gatgtacctc cttattgttt tatatagctc gtcggaatcg
     7381 agaggctttg tgatgaatgc gttgcatccg gcatctaagc tacgctgctc ttcattagcc
     7441 atgctgctgg ctgttgtggc aactatcgga atgtcatgat tttgttttct aatttcccgg
     7501 acaaccctat atccatccat aaatgggagt cgaagatcca tgagtataag gcttatttcc
     7561 ggatgaagta gggtaatttc aactgcatcc tccccgttct tagcatgtat caacctcacg
     7621 tttgtgcgtt tcagcagggc ttcgatgtac agaaaattca tgaggtcatc ctcaacaacc
     7681 agaatggttt taccactcca attctcgggg ctacaaaaca attcgtttga ctgtttcttt
     7741 tttgaaatat tcaggggtag tcgtaggtaa accacatttc ccgcatcagg cgttgagcca
     7801 aagtctaact ctccacccaa cgccttggcg taaccattgg caattatcat tcctatttcg
     7861 gcttcaccct gttgtcttgt gaaatgtttg ccattagacc ttactgattg tctaagaatt
     7921 atttcgtttg atgatgtgtc ggtaaagggc tgctttactt cccgaacatg gaaggtaatg
     7981 cttgcattgg aatcaactct gtagccaatt tcaagggtgt cggattcggc aagttttgat
     8041 aattttccca gaatgtgcag cagcgttttc ttgagcttat cggcatcggt aaccataatg
     8101 gcctgctcgt catcaagcga tagcgcaagt tttagggcta cctgacggct catgtactcc
     8161 ctattttctg taagttggtg taaaatatca cttattagct tattaatgtt gcagctggcg
     8221 gggagaactt cgagctggtt ggcttctatt ttggctaggt caaccatttc gtcggccaga
     8281 aggataattt tattgcaaag ctgctggatt tgttgagccg cagcatcctt ttccttcttg
     8341 gtggtgttat tcataagaag tgcttgcgaa aatccaagca gcccatttag ggatgtccgc
     8401 attctatggt tcaaatcgac ccaaaagctg gttttgcact ccgttagttc gctcaacttt
     8461 tggagggtat cattcagctt gtcggtaagg gttgcagaaa tttcttcctg cctggcattc
     8521 atggtttgaa gctgccttat cgtcgccagt aactccttgt tattattttt taattgtttg
     8581 ttgcggtaga gcagaaggcc tatcacgagc aacattccca acaaaacggc catgcccacc
     8641 atgttggggg ttgccgttaa ggtttgcaaa aggatgttta atttatccgg tatcattcag
     8701 gatacgccat ttgcaattaa aattgttgag aaatttgctg ttattttaag gccgaatagc
     8761 cgctaccatt caactttggg gttataagtc aacataaagt ttgactgcta gaggtattaa
     8821 caggggattg ctaaatgatt cggagcgaat ggatatggtt tttagggtgc tagctgtggg
     8881 caaaagtaac tttcgttcaa gtcattatca aaacgcccat ctggttttta ggcgaaacac
     8941 gtcaaaaatt tgatagaact tgatttagtg gagattaact tttcgttact cctgtcggga
     9001 tggcaagaag acctgatttt tggcttatat agttgattat ctgcccataa cggtgtagct
     9061 actccctgat aatccggaca agtccgccat cgcccagggc tataatacca gaaggtttgt
     9121 ccgatacagg atggtcgtag gtttgcggga caatgaaatc cactcccgat ttgatcacct
     9181 catcaacttc ggagaagttt ttggggaatg gccgtccgga tatgttggca gaggttgaca
     9241 caatgggctt tccgaactgc aggattagct tctggcagaa atcgtgcctt accaccctaa
     9301 tgccgatgct tccgtcgggg gcaatgaggt tgggcgccag attttttgcc ttttggaaga
     9361 tgatggtcag gggtttatcg gccagctcaa ccaggtccca ggctacttca ggtacctcgt
     9421 caacgtagga tggaattctg ccgggttggt caaccagcac aatcatgctt ttgctatcgt
     9481 cgcgctgttt caacctgaat attttgtcaa ccgctgcagg gtttgtagca tcgcagccaa
     9541 tcccccacac cgtatcggtt gggtagagga tgatgcctcc cgatttaagc gtgtctatag
     9601 ttctgcgtag gtcggcatcc attgaattca tggtttatta atttgtggtt ttgggccaaa
     9661 taactttttt atcttatgcc tgcggtataa attcaacttt ctggaatcag gaattccatt
     9721 tctttcaatg aaattttcta cggcgttaac cattcttgct gatgattttc catcagagta
     9781 gggatggtag gcttcaatta cccgcttacg ttgtgcggca aattggctgc actccagctc
     9841 ctgttccaca actgttatca gatcttccgg tttcagaata ttctgccatt gtatggtttc
     9901 agcgttggag caaatagtta ctaccggttt atcgagtagc aaaaactcgt acactgctga
     9961 tgacgtatcg ctaatcatta ggtcggcaag cgaaagatat ttaatgatat tgctgtcctc
    10021 tactacttct acattatcaa atatttttgc ttttgtcttg tatgtttcta ctgtttcaga
    10081 gttcattaaa tcatgaaatt ttaccattag gtagatattc agcttgcagg caaggttaaa
    10141 gatgtggtct atagctccgg gtgcggacgt aagtgaagga gaaaaggtgg gtgcataaag
    10201 cacaattttt tgcttcccat gtttttgaag aattgttgtt ttttcgttac cgattgttga
    10261 ggccagcctg taaagcggat caagcttaca ccatccggtt tcgaccactt caaaattgcc
    10321 atagtgattc gaaagctctg taaaccgttt tgtgaagtat ggtccctgag ttaggtaaag
    10381 gtcgaagtac ccgcggattc tgaaatgtcc ctttttttct ccagcaagcc catgaaaaac
    10441 ttgaacttta acacctttta gatagttagg gacctcatta cctggaacaa aaatagcatc
    10501 acttttatat acataaacct ctttaattga attgaaaacc tttacctcgt ttatgaaggg
    10561 acacttgggt gctatacgtt ccggtatgta ccaaacaact tcatgatgca gctcaaccag
    10621 cgtgtcgtga aggggtttta gtattccaaa agcatagggg ttggcacaaa aaagaacggc
    10681 tttcaacata tttaaatttt cgggttgtgg ggctacttaa ataaccgatt agcgttctcc
    10741 aaatcttcgc gaaaatcaat ttccatgcag cgcatggagg aaacatcgtg gggtaacact
    10801 ttgattttat ccttctctat tgccagttct aacccgcgtt caaagtagtc ggagttttca
    10861 cactcctcaa gccttgaaat aaatgccctt atatcgttgc tgctgataaa gttgatgcct
    10921 actgcctcgc caagggcatg ttttaccttt ttcgaaatat tcttaataaa tccgaattcg
    10981 tccaggttgt actttacttc ctcctcgcca acggcgctgt tgttcacaca aacaaacgag
    11041 ttgttattct caatatgggt tttaatttcg ccaagtatgg cagagtcaaa aaccacatcg
    11101 ccgtttagcc acagcaccga ctcgttgcga tgctttctta gtgctttcag aaggcttttg
    11161 gaagtattgg ttacgtcaaa gttctggttg taaatgtagg atatgtcggg gaagttttcc
    11221 attatgaggt cttttttaaa accaaccaca atgttgatgt cgtcggggtt gaaatattcc
    11281 gtaaggttat ccagctgctg ctgcatgatt gttttaccgc tttgcagaac tgtaagcggt
    11341 ttgggaagcg gattccccaa ccttga
//
LOCUS       DAOSHM010000071        11261 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_4874_length_11261_cov_19.868910, whole genome shotgun
            sequence.
ACCESSION   DAOSHM010000071 DAOSHM010000000
VERSION     DAOSHM010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 11261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11261
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4874_length_11261_cov_19.868910"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            612..875
                     /locus_tag="PK839_09185"
     CDS             612..875
                     /locus_tag="PK839_09185"
                     /inference="COORDINATES: protein motif:HMM:NF024590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOG72948.1"
                     /translation="MEKPVLIEMFYTLSCPNCRTLKRMLDEILPTFGNKFEFKRTMAN
                     SPVGMIRTMKLGIGAVPTLLINNEIVFRQVPTKEELINKLNQY"
     gene            894..1127
                     /locus_tag="PK839_09190"
     CDS             894..1127
                     /locus_tag="PK839_09190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72949.1"
                     /translation="MEAQKKLRCPLGIPGGILVALIGLVGIVLNIINFDWFELLQSIA
                     LLLLGLPFVRVTMMVHLGNDRLDALEDKVNGKK"
     gene            1150..1536
                     /locus_tag="PK839_09195"
     CDS             1150..1536
                     /locus_tag="PK839_09195"
                     /inference="COORDINATES: protein motif:HMM:NF024953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HOG72950.1"
                     /translation="MKKIIYWTSTVLISLMMLSSAWGYFTNPDMKGAFVHLGFPDYFR
                     IELGTAKFLGVLALLIPMIPARIKEFAYFGFTITFISAIIAHLSSGDPIMAVVFPLVA
                     QVLLTVSYIYFILISDKKMTMQEAES"
     gene            1624..2181
                     /locus_tag="PK839_09200"
     CDS             1624..2181
                     /locus_tag="PK839_09200"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HOG72951.1"
                     /translation="MIKVGIIIGSTRPGRNAEAVAKWVYEHARRRTDAEFELVDIKDF
                     NLPVLDEPVPASMHQYMKDHTKVWSDKISSFDAFIFVTPEYNHATTGALKNAIDYLYV
                     EWNNKAAGFVSYGSTGGVRAVEQLRLIMGELQVADVRTQVALSLFTDFENFSVFKPAA
                     LHEDNLKAMLDQLVAWGGALKTIRK"
     gene            2186..3010
                     /locus_tag="PK839_09205"
     CDS             2186..3010
                     /locus_tag="PK839_09205"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HOG72952.1"
                     /translation="MNGSDKLPVIFIPHGGGPWHIIPDAMGDPVGYGNLRGYLKELGN
                     SYRSRIRSILMVSAHWEETFPTIHFGESPNLYYDYYGFPEYTYHLSWPAPGNPNLALR
                     VEQLLNNKGFNTRREMIRGYDHGTFVPMMVAFPEAQLPVVQLSLVRGLDPSTHIAVGQ
                     ALEPLRSEGVLIIGSGMSYHNMQGFMSGSPEVESTSKKFDSWLAAAVEVEVKNPEQRI
                     QTLVKWAEAPGAFESHPRSEHLVPLFVVAGAAGSDAGKRSYNGKLMSATISGFTFG"
     gene            3081..3530
                     /locus_tag="PK839_09210"
     CDS             3081..3530
                     /locus_tag="PK839_09210"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOG72953.1"
                     /translation="MELESEVKGHFRNEYHKGLINLIYTVNLLSYEFLQSLKNHGVTE
                     QQYNVLRILRGFRSQAPVSLGFIKERMLDRNSDVSRIIDKLQRRGLVSRDVSSCDRRQ
                     KAIQITDKGLNLLSSMLDCEKKVDLLLNNLTPDELRQLNQLLDKIRG"
     gene            3842..6430
                     /gene="clpB"
                     /locus_tag="PK839_09215"
     CDS             3842..6430
                     /gene="clpB"
                     /locus_tag="PK839_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011966358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HOG72954.1"
                     /translation="MNLNNFTIKSQEAIQKAIEIAQGYNHQAVEPAHLLKAILSESEN
                     ITGYLLRKLGVNPATFDEVLDRMLDSMPKVSGGEQYLSSSTSKVLQRALEFSKSMGDQ
                     FVSVEHILLGLIVAGEQVGQMMKDAGMTEKEMKLAIEELRKGSKVNSQTAEDTYNALN
                     RFAINLNEMARSGKLDPVIGRDEEIRRVLQILSRRTKNNPILIGEPGVGKTAIAEGIA
                     HRIVSGDVPENLKTKQIFSLDMGALVAGAKYKGEFEERLKSVVNEVISANGEIILFID
                     EIHTLVGAGKGEGAMDAANILKPALARGELKAIGATTFEEYQKYFEKDRALERRFQSV
                     VVDEPLPADAISILRGLKERYENHHHVRIKDEAIIAAVELSHRYITSRYLPDKAIDLI
                     DEAASKLRLEMNSVPEDIDEIERRIKQLEIEREAIKREGDKAKLEDLSREIAELNEER
                     TKLRAKWQNEKSIIDRIQKNKQEIENLRLEAEEAERRGDYGRVAEIRYGRIKQAESEI
                     EQMKVELKGVQADFALIKEEVDAEDIAEVVSRWTGIPVSRMLQSEREKLLHLEEELHK
                     RVVGQDEAIAAVADAVRRSRAGLQDSKRPIGSFIFMGTTGVGKTELAKALAEFMFNDE
                     NLMTRIDMSEYQERHSVSRLVGAPPGYVGYEEGGQLTEAVRHKPYSVVLLDEIEKAHP
                     DVFNILLQLLDDGRLTDNKGRTVDFRNTIIIMTSNIGSHIIQENYASLTRSNANEVFE
                     RTRQQVFDLLKESIRPEFINRVDEIIMFTPLSHHEIKQIVMLQFNRIKDMLAQNGVKL
                     EISEAAVEWITNAGFDPVYGARPIKRVMQKYILNELSKQILAGSIDRDKTITVDYFGE
                     DVLVFR"
     gene            complement(6612..7880)
                     /locus_tag="PK839_09220"
     CDS             complement(6612..7880)
                     /locus_tag="PK839_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005805768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HOG72955.1"
                     /translation="MFALADCNNFYASCERVFDPSLNGKPVVVLSNNDGCIIARSNEA
                     KALGLKMGEPAFKIKDIIEKNRVAVFSSNYTLYGDMSQRVMNTLSGFTPEIEIYSIDE
                     AFLSLHGFTHFNLLEYGHKIRSVTTQNTGIPISIGIARTKTLAKVANHFAKKQPNYGG
                     VCLLGSEAERVEALKHFEIGEVWGIGRQYNKLLNRYGVSTAYDFTKLPTEWVRRHMSV
                     VGLRTQKELMGIPCLDLEHTAPPKKSICTSRSFGQMQTELGYLEEAVASFASSCAHKL
                     RKQQSVATLVMVFAHTNFFRDDLPQYSASKIATLPVATNSSMEIVHYALQALRSLFKP
                     GYRYKKAGVIVSGIQNSSGVQTALFDTVNRNKHSLAMAAMDIINDRFGPSTIKLAAQG
                     TGRKWRLRQEKLSPFYTTRWDQLITVNAER"
     gene            complement(7882..8322)
                     /gene="umuD"
                     /locus_tag="PK839_09225"
     CDS             complement(7882..8322)
                     /gene="umuD"
                     /locus_tag="PK839_09225"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HOG72956.1"
                     /translation="MKLVKSTQTIDFYTPDLNAELTLPYADGIRAGFPSPAQDYLDLA
                     FDLNRELVKNPSSTFYGRVRGNSMVDAGINDGDILVIDKSIPPADGRRAVCFIDGEFT
                     LKTLKVEKDILWLLPANPKYKPIKVTPESNFTVWGIVTYVIHKV"
     gene            complement(8332..8700)
                     /locus_tag="PK839_09230"
     CDS             complement(8332..8700)
                     /locus_tag="PK839_09230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72957.1"
                     /translation="MEIEKIVETIISNAKGLTNDELTKCRSEYDPIYLNDRITHHRKI
                     SNRQNITLFLLVIFAILAFVVVVFGAKVESGTMSWIRGVVTGYFVAIIFLMPKTFKNH
                     SSIASTLSLIKTLREEQKKM"
     gene            8989..9954
                     /locus_tag="PK839_09235"
     CDS             8989..9954
                     /locus_tag="PK839_09235"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HOG72958.1"
                     /translation="MKIAVAFRTDPDQRKYLVDELGGETELIFLNDLAEDKKMEVLSE
                     VEIILSWNPEKEGITNSKNLGSNLKFIQLMSAGYDHVNPDDFPSNVKIAANQGAYAEP
                     MAEHALAMILALSKRLPLYHQHLVKGEFNQLKSPTKSLRGSVLGIIGFGSIGKATARL
                     AKPFGVKVMALNTSGKTDEEVEYIGTLNDLEYVLRNADSLLISCALNEQTEGLINRQA
                     LELMKPDAVLVNVARGPIVVEKDLYEHLKGHPNFYAGIDAWWIEPFKYGRFELHYPFF
                     ELPNIIGSPHNSAMVENSMIIGTEKAVANLRRFINNEAVRGLINR"
     gene            9977..10711
                     /locus_tag="PK839_09240"
     CDS             9977..10711
                     /locus_tag="PK839_09240"
                     /inference="COORDINATES: protein motif:HMM:NF013488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HOG72959.1"
                     /translation="METHSTTSNNIKVEIWSDVVCPFCYIGKHRFESALEKFQHSNIV
                     EVEWKSFQLDPYAVTNSNLSVMDNLAREKGLSAAQVQESVGYIADMAKGVGLDFHFEK
                     AIVANSFKAHRILHLAKTHGKQNELEEKLFAAYFTQGMNIDDDDVLLNLATSVGLSGD
                     EVRKVLGSDAYSDEVKHDIAHARQLYVRGVPYFLFDGKYAVSGAQEVDFFLQTLNKAY
                     ADHKGQEVIEIPGNNPGVCGPDGCSI"
     gene            10731..11207
                     /locus_tag="PK839_09245"
     CDS             10731..11207
                     /locus_tag="PK839_09245"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HOG72960.1"
                     /translation="MDKPRSFEIKSQSKGIQLPDDVIPFDLKHVSDSFVSGRQQRYIE
                     IQYFRGSKPENFYARVLFRKEAQGAPDIVHGGAIAAVFDETMGAICLVLGQPAVTATL
                     TVDYRIPVPVEKEFLVEARLEKTERRKFFITARMLDEQQELFAESKAIYVKTKNDG"
BASE COUNT         3048 a         2385 c         2838 g         2990 t
ORIGIN      
        1 agcaaggggc gcagcccctt gtatgaagtg aacgattttt acaagccctg aaggggcgga
       61 ataggtttga aacgaagagc ctgaagagga tgtgttggcg atgcaagtag tggcttttcc
      121 aaagagctgt tcagaaccgt tacatcgcct tcacaaaatt gtggcaaaga gtcatctaag
      181 tgttctttct tgtcttgata caagaaagaa ccaaagaaaa tcatgtccga aaagccagac
      241 ccatttgcgg gtcaggcttt tatgccattt tactaatacc agcaatgttc aacgattccc
      301 ggctttacga gcactgcgga gtataccctt ctatggtcag agttattcag aataagctgg
      361 agtatgagga ggttagatga agcaagatga agctataggg ggtgaagata gccatttaac
      421 cgtcaggaac gacgagggaa agcttaacac tttctctctc gttcttgaaa aattagtttt
      481 ttttcgtttg ccccttaagc taacgcccta aaaagttgca tttgcaatca aaatctaagc
      541 aaaaaaatta atggatgttg caacaaataa ctacttggct tgttactatg acaaaaattt
      601 aaatgaaaac aatggaaaaa cccgttttaa ttgaaatgtt ttacacgctc tcttgtccca
      661 actgtcgcac actaaagcga atgctggatg aaattctccc aacttttggg aataaatttg
      721 agtttaagcg caccatggcc aactccccgg ttggtatgat tcgaaccatg aagcttggta
      781 taggtgctgt tccaacattg cttatcaaca acgagattgt ttttaggcag gttccaacaa
      841 aagaggagct tataaataaa ctaaatcaat actaacctta aattttaatt acaatggaag
      901 cacaaaaaaa acttcgctgc ccactaggta ttcccggcgg aatattagta gccctaatcg
      961 gattggtggg aattgttcta aacattatca atttcgactg gtttgagctg ctacagtcaa
     1021 tcgccttact gctactcggt ttaccttttg ttagggtaac catgatggtt catttgggca
     1081 acgataggct tgatgcactt gaggataaag taaatggtaa gaaataatca aggaagaaat
     1141 attatgaggg tgaagaaaat tatttactgg acaagcaccg tgctcatatc gttgatgatg
     1201 ctgtcgagtg catggggata ttttactaat ccggatatga agggagcgtt tgtccacctt
     1261 ggatttccgg actacttcag aatagagttg ggaacagcaa aatttctggg agttttagcc
     1321 ctgctgattc ctatgattcc tgctcgaatt aaggaatttg cctactttgg gtttaccatt
     1381 acctttattt cggccataat tgcgcatttg tcgagtggtg atccaattat ggcagttgtt
     1441 tttccgctgg ttgcccaggt actgcttacc gtatcctaca tctacttcat cctgatttct
     1501 gataaaaaga tgacaatgca ggaagccgag agctaagcaa ataatgatat gttgccctgg
     1561 ttatctgggc aatagtgaat acagaacggt ttaattttaa taacactaaa caatcttgaa
     1621 gtaatgataa aagttggaat tattattgga agcacaagac ctggcaggaa tgccgaggct
     1681 gttgccaagt gggtttacga acatgcccgc aggagaaccg acgctgagtt tgagctggtt
     1741 gatattaagg acttcaacct tcctgttctc gatgagcctg ttcctgcctc catgcaccag
     1801 tacatgaagg atcacactaa ggtgtggtca gataaaatca gctcgtttga cgcttttata
     1861 tttgtaaccc cggagtataa ccatgccaca accggagcgc taaagaatgc aattgactac
     1921 ctctacgttg agtggaataa taaggcggca ggttttgtca gctatggttc aaccggcggg
     1981 gttcgggctg ttgagcagct tcgtctcatc atgggtgaac ttcaggtggc cgacgtgcgt
     2041 acccaggtgg ccctgtcgct ttttaccgat tttgaaaact tcagcgtttt caaaccggct
     2101 gcacttcatg aggataacct caaagccatg cttgatcagc tggtggcttg gggcggtgct
     2161 cttaaaacca ttagaaagta ggattatgaa tggaagcgat aaacttccgg tgatattcat
     2221 tccacacggg ggaggtccat ggcatataat acccgatgcc atgggcgatc ccgttggcta
     2281 cggaaacctt agaggctacc ttaaagaatt ggggaattca tacaggagca ggattcgatc
     2341 cattctgatg gtttctgctc actgggaaga aacgtttccg accatacatt ttggcgaatc
     2401 gccaaatcta tactatgatt attacggttt cccagaatac acctaccacc ttagctggcc
     2461 tgcaccgggt aatcctaacc tggcactgag ggttgagcag cttcttaaca acaaggggtt
     2521 taacacccga cgagagatga tcaggggata cgaccatggt acatttgtac ccatgatggt
     2581 tgcatttcct gaagctcaac ttcctgttgt tcagctttcg ttggtccgtg ggcttgaccc
     2641 ttccacccac attgcagttg gacaagccct tgagccactc cgttctgaag gagtcctcat
     2701 catcggctcg ggcatgagct accacaacat gcagggcttc atgtcgggct ctccggaggt
     2761 agaatccacc tctaaaaagt ttgacagttg gctggccgca gccgttgaag ttgaagttaa
     2821 aaaccctgaa caacgaattc aaacccttgt aaaatgggct gaagcgcctg gcgcttttga
     2881 gagccatccc agaagtgagc acctggtacc actttttgtt gttgccggtg cagcgggtag
     2941 tgatgcaggt aaaagaagct acaacggaaa gctgatgagc gccaccattt cgggctttac
     3001 atttggttaa taatccttgg gcaagcataa gactaaattg tggcgtcgtt ttttttcaga
     3061 aacttaatcc tctttaaaac atggagctgg aaagcgaagt aaagggccat tttaggaacg
     3121 aataccataa gggacttata aatttgattt atacggtgaa cctgctgagc tacgagtttc
     3181 tccagtcgct taaaaaccat ggagttaccg agcagcagta caacgtgctt agaattttac
     3241 gtggctttag aagccaggca cccgtatcgc ttggatttat taaggaacga atgctcgata
     3301 ggaattctga tgttagccgg attattgaca aacttcaaag gcggggcctg gtatccagag
     3361 atgtcagcag ttgcgacaga cgccagaaag ccattcagat tactgataag ggtttaaact
     3421 tgcttagcag catgctcgac tgtgaaaaaa aggttgacct gttactaaat aatctcactc
     3481 ccgacgaatt gaggcagctt aatcagctcc tcgacaaaat taggggctga cctgccctaa
     3541 atcatacact tcccgactgt ttttcttatt ctgtatcaga aaactttttg aacgccatcc
     3601 attctggctg ttcattatgc tggaaccgtt taaaccgtaa aatcccgaag tgggcttacg
     3661 gttgtttttt attataatgt gcttgaatat agtgcattac gcaaaattag ggatattcct
     3721 gatttctccg cactgtcata atttcctatt tgcgctttgt gcttatgtca acatggcatt
     3781 atttgggtta tggtagatgt tttgctggct tatggtgata aatttaagga ggaactatag
     3841 tatgaacttg aataatttta ccataaaatc gcaggaggcc attcagaagg ccattgagat
     3901 tgcgcagggg tacaaccatc aagctgtaga acctgcgcac ttgctaaagg ctattttgag
     3961 tgagtccgaa aacattaccg ggtaccttct tcgaaagctg ggtgtaaacc ctgcaacatt
     4021 tgatgaggtg ctggaccgga tgcttgattc catgccaaag gtttcgggtg gcgagcagta
     4081 cctaagctcg tcaaccagca aggtgctgca aagggcactg gaatttagca agtccatggg
     4141 cgaccagttc gtgtccgtgg agcatatcct gttggggtta attgtggccg gtgaacaggt
     4201 tggacagatg atgaaggatg ccggaatgac cgaaaaggag atgaagctgg ctattgagga
     4261 gttgagaaaa ggatcaaagg tcaattcaca aacagccgag gacacctaca atgcgcttaa
     4321 cagatttgcc ataaacctca acgagatggc gcgaagcggg aagctcgatc cggtgattgg
     4381 tcgtgatgag gagataaggc gcgtgctgca gattctctcg cgtcgtacca agaacaatcc
     4441 tattctgatt ggtgaaccag gcgttgggaa aactgccatt gccgagggaa ttgcccaccg
     4501 tattgttagc ggcgacgtgc ccgaaaacct caagaccaag caaatttttt cgctcgacat
     4561 gggggcgctg gtggccggcg ccaaatacaa gggcgagttc gaggagcggc taaaatcggt
     4621 ggtaaacgag gtgatttcgg ctaatggaga aattatcctg tttattgatg agatacacac
     4681 cctggtaggt gctggtaaag gtgagggtgc tatggatgcc gccaatatcc ttaaaccggc
     4741 attggcaagg ggcgagttga aagctattgg agccaccact tttgaggagt accaaaagta
     4801 ttttgaaaag gacagggcgc ttgagcgtcg tttccagagc gtagtggttg acgaacccct
     4861 tcctgccgac gctatctcaa ttctgagagg gctaaaggag cggtacgaga atcatcacca
     4921 tgtgagaatt aaggacgagg ccattattgc agccgttgag ctgtcgcatc ggtacatcac
     4981 cagccgctat ttgcccgata aggccataga cctgatcgac gaggccgcct caaagctgag
     5041 gttggaaatg aattctgttc cggaggatat agatgagata gaacgtcgca ttaagcagtt
     5101 ggagattgaa cgtgaggcaa taaaacgtga gggcgacaag gccaaacttg aggacttatc
     5161 acgcgaaatt gccgagctaa acgaggaacg cacgaagcta agagccaagt ggcaaaacga
     5221 gaaaagcata attgacagga ttcagaaaaa caagcaggag attgagaacc ttcggctcga
     5281 ggctgaggag gctgaacgta ggggcgatta cggaagggtg gctgaaatcc gttatggcag
     5341 aataaagcag gccgaatcgg aaattgagca gatgaaggtg gagctgaagg gcgttcaggc
     5401 cgactttgcc ctgataaaag aggaggttga tgcggaggac attgccgagg tggtttcgcg
     5461 gtggacgggc attccggtaa gccgcatgct gcaaagtgag agggaaaaac tacttcacct
     5521 ggaggaggag ctgcataagc gagttgttgg tcaggatgag gcaattgcgg ctgttgccga
     5581 tgcagttagg cggagccgcg cagggctgca ggattccaag cgacctatcg gatcgttcat
     5641 cttcatggga actacaggcg ttggaaaaac cgagctggcc aaggctctgg ccgagttcat
     5701 gtttaatgac gaaaatttga tgacccgtat tgacatgagc gaataccagg aaaggcactc
     5761 cgtatcaagg ctggttggtg cacctcccgg atatgttggc tacgaggaag gtggacagct
     5821 taccgaggcg gttcggcata agccctactc ggtagtcttg cttgatgaaa ttgagaaggc
     5881 tcatcctgat gtattcaaca tactgcttca gctgctcgac gatggccggt tgactgataa
     5941 taagggacga actgttgact tcaggaacac catcatcatc atgacgtcaa acatcggttc
     6001 gcacatcatc caggaaaact acgcttcgct tacacgaagc aacgccaacg aggtgtttga
     6061 gcgaacccgt cagcaggtgt tcgacctgct aaaggagtcc attcgtccgg aattcatcaa
     6121 cagggttgac gagattatca tgttcacccc gcttagccat cacgagatta aacagatcgt
     6181 tatgctgcag tttaaccgta taaaagatat gctggctcag aacggtgtta aacttgagat
     6241 ctccgaggca gctgtggagt ggataaccaa tgccggtttc gacccggtgt acggcgctcg
     6301 tcctataaaa agggtgatgc agaagtacat ccttaacgag ctttctaagc aaattcttgc
     6361 tggcagcatt gacagggata agaccattac ggttgattac tttggtgagg atgtgctggt
     6421 gttcagatag tttttaggct agtttcatat ttagcaatac ctatagccgg aataaccccc
     6481 agggcaattc cggctttttg gtatggcaca tgcgttaagg gaaaatggcc tgttaccatt
     6541 tcctttccaa aatttgctgt tgagggtttt accgtgttcg tccttacgct tcatggtcag
     6601 gttctacttc ttcacctttc agcattaacc gtgataagct ggtcccagcg tgtggtgtag
     6661 aagggcgata gtttttcctg tcttaggcgc cattttcgtc cggttccttg ggctgcaagt
     6721 ttaattgtgc tggggccaaa acgatcgtta ataatatcca tggcagccat agctaggcta
     6781 tgcttattgc ggttgacggt gtcaaacagg gcggtttgca ccccgctact gttctggatt
     6841 ccactaacaa ttacccctgc ctttttatac cggtaccctg gtttgaatag gctacgaagc
     6901 gcctgcaggg cgtagtggac aatttccatg ctgctgttgg ttgctaccgg tagggtagct
     6961 attttgctgg cggagtactg gggtaaatca tcgcggaaaa agttggtgtg ggcaaaaacc
     7021 attaccagcg tggcaaccga ttgctgctta cgtagcttat gcgcgcagct gctggcaaat
     7081 gaggcaacgg cctcctcgag gtaacccagt tcggtttgca tctgaccgaa tgatcgtgag
     7141 gtgcaaatgg actttttggg aggggcagtg tgttcgagat ccaagcacgg aatacccatc
     7201 agctcctttt gggttcttag tccaacaacc gacatgtggc gccttaccca ttcggttggg
     7261 agtttggtaa agtcgtaggc tgtactaaca ccgtaccggt taagaagctt gttatactgc
     7321 cggccaatgc cccaaacctc cccaatttca aaatgcttca atgcttcaac ccgttcagcc
     7381 tcgcttccca gcaggcatac gccgccgtag ttaggctgct ttttggcaaa gtggttcgcc
     7441 acctttgcca gggtttttgt gcgggcaatg ccaatactta tcgggatacc ggtgttttga
     7501 gtggtaacgc tacgtatttt atggccatac tccaacaggt tgaaatgggt aaatccgtgc
     7561 aggctcagga aagcttcatc aatggagtaa atttcgattt ccggggtaaa gccggacagc
     7621 gtattcatca ccctttggct catatcgccg tagagcgtgt agtttgatga gaaaacggct
     7681 accctgtttt tctcaatgat atctttgatt ttaaaggcag gctcacccat ttttaatccc
     7741 agggcttttg cctcgttact tcgggcaatt atgcagccat cgttgttgga gagtaccact
     7801 acaggcttgc cgttaagcga agggtcgaaa accctttcgc atgaggcgta aaagttgttg
     7861 cagtcggcca gagcaaacat actaaacctt atggattaca taggttacaa taccccaaac
     7921 cgtgaagttg ctctcgggcg taaccttaat gggtttgtac ttagggttag ccggcagtaa
     7981 ccaaagtata tccttttcaa ccttaagggt tttgagggta aactcaccat caatgaagca
     8041 aaccgccctt cggccatcgg ctggcggaat agatttatca ataaccagaa tgtctccatc
     8101 gttgattcct gcatcaacca tcgagtttcc acgcaccctg ccgtaaaagg tggagcttgg
     8161 gtttttaacc agctcccgat tcaggtcaaa ggctaagtcg agataatcct gtgcgggcga
     8221 ggggaatcct gccctaatgc catctgcata cggtagcgtt agctcggcgt ttaagtcggg
     8281 tgtgtaaaaa tcaatggttt gtgttgattt taccagcttc atgggtttat gctacatctt
     8341 cttttgttcc tcacggagcg ttttgataag gcttagcgtg ctggcgattg aggagtggtt
     8401 tttgaaagtt ttgggcatca ggaatataat ggccacgaag tacccggtta caacacccct
     8461 aatccagctc attgttcccg attctacttt tgccccaaaa acaacaacca caaaggcaag
     8521 tatggcaaaa attaccagca ggaaaagggt gatattttgc cggttcgaaa ttttgcgatg
     8581 gtgggttatc ctgtcattca ggtagattgg gtcatattcc gatctacatt tagttagctc
     8641 atcatttgtt agacctttcg cattcgaaat tatggtctcc acaatctttt ctatttccat
     8701 agcagcttta gttttgatta acaaatttac tcctattttt ggtatgccga attatttctg
     8761 gcgaacaata acaaacctgt aatgtttaca agtcattggt agtagaaagg atggaactcc
     8821 atggaagctt cctgcaagga atttctttac gattatattg aaggcgttct aacctaagta
     8881 gtaatgctgt ttacaggtgt tattatgagc gtatgtaatg tataaatgtg attttcaagg
     8941 ccaagtttgt aaagcattaa ttgttagtat agtaaatatt ttgtaaggat gaaaattgca
     9001 gttgccttta gaactgatcc cgaccaaagg aagtacctgg ttgatgagct cggtggggaa
     9061 acggagctaa tttttctgaa tgacttagcg gaggataaaa aaatggaagt tctttcggaa
     9121 gtcgaaatta ttttatcgtg gaatcctgaa aaagagggaa tcactaatag taagaattta
     9181 ggtagcaacc tgaaatttat tcagctgatg tcggcgggct acgaccatgt aaaccctgac
     9241 gactttccct ccaacgttaa aattgctgcc aaccagggtg cctatgccga gccaatggct
     9301 gagcatgcat tggccatgat actggcatta agcaagcgct tgccgttgta ccatcagcac
     9361 ctggttaagg gtgagttcaa ccagctaaaa agtcctacta aatcgcttag gggctcggtg
     9421 ctggggatta tcggttttgg ctcaattggc aaggctaccg caaggttggc taaaccattt
     9481 ggtgtaaaag taatggcctt aaacacctcg ggaaaaaccg acgaggaggt ggagtacatt
     9541 ggaacgctga atgatttgga atacgtgctc aggaatgccg atagcctgct aatttcatgc
     9601 gcacttaacg agcaaaccga agggttgatt aaccgtcagg cacttgagct gatgaaaccc
     9661 gatgccgtgc tcgtaaatgt tgcccgcggc ccaatagttg ttgagaaaga cctatacgag
     9721 cacctgaaag gccatccaaa tttttacgca ggtatcgacg cctggtggat tgagcccttt
     9781 aagtatggta ggtttgagct gcactatccg ttttttgaac ttccaaatat tattggttca
     9841 ccccataact cggccatggt ggaaaattcc atgattattg gaaccgaaaa agccgtggcc
     9901 aacctccgga ggtttattaa caacgaggct gttagaggac ttatcaacag gtagagtatt
     9961 aaccataaat acaaaaatgg aaacacatag cactactagt aataacatca aggttgaaat
    10021 ttggagcgat gtggtttgcc ccttctgcta cattggcaag cataggtttg aaagcgccct
    10081 tgagaagttt cagcattcta acatcgtaga ggttgaatgg aagagcttcc agctcgaccc
    10141 gtatgcggtt actaactcta accttagcgt tatggacaac ctggcccggg agaagggtct
    10201 gtcggcagca caggttcagg agtcggttgg ctacatcgcc gatatggcga agggcgttgg
    10261 gctcgacttt cattttgaaa aagcgattgt tgctaactcc tttaaggcgc acagaattct
    10321 acatctggcc aaaacccacg gaaagcaaaa cgagctggag gaaaagctgt ttgcagccta
    10381 cttcacccaa ggtatgaata ttgatgacga cgatgttttg ctgaacctgg ccacatcggt
    10441 ggggctaagt ggagatgagg tgcgcaaggt gctgggtagc gatgcttact ccgatgaggt
    10501 gaagcacgat attgcccatg cacgccagct ttacgttagg ggcgtgccct actttttgtt
    10561 tgatgggaag tatgccgttt ctggtgctca ggaagtggat ttttttcttc aaacgctcaa
    10621 caaggcttat gccgaccata agggtcagga ggtgattgaa attccaggca acaaccccgg
    10681 ggtatgtggt cctgatgggt gcagtattta aataaattag gcagtgaacc atggacaaac
    10741 ctagaagttt tgaaatcaaa agccagtcaa agggaatcca gctgccggat gacgtgattc
    10801 cttttgattt gaaacacgtt tcggattcgt ttgtttcagg aaggcagcag cgctacatcg
    10861 aaatccagta ctttcggggt agcaaacccg aaaacttcta cgccagggta ctcttccgca
    10921 aggaagctca gggtgcgccc gacattgttc atggtggcgc catagccgca gtgttcgatg
    10981 aaaccatggg agccatatgc ctggtgctgg gccagccagc tgttaccgca accttgacgg
    11041 tagattaccg aatacccgta cccgttgaaa aggaattttt agttgaggca cggcttgaaa
    11101 agaccgaaag gcggaagttc tttattacag ccaggatgct cgatgagcaa caagaacttt
    11161 tcgccgaatc aaaggctatc tacgtgaaaa caaaaaacga cggatgaccc gtcaaaattt
    11221 tctgttgcat ttccgtcggt ttaagatagg ccgctataat c
//
LOCUS       DAOSHM010000072        10890 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_5072_length_10890_cov_20.151546, whole genome shotgun
            sequence.
ACCESSION   DAOSHM010000072 DAOSHM010000000
VERSION     DAOSHM010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 10890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10890
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5072_length_10890_cov_20.151546"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(27..1217)
                     /locus_tag="PK839_09250"
     CDS             complement(27..1217)
                     /locus_tag="PK839_09250"
                     /inference="COORDINATES: protein motif:HMM:NF013347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HOG72961.1"
                     /translation="MVEKFEMIAKTLQGLEGVLADELTELGAEDVKIENRAVSFRGTT
                     ETLYKANFWLRTAIRVLKPVYSFSARTIEEFYGNARKFDWTSLMDLSQKFAVDSVVNS
                     EVFPHSRYIALKFKDAVVDQFRDRYGKRPFVDTKNPQIQFHVHISGDSCTILLDSSGE
                     SLHRRGYRTAQDIAPINEVLAAGLIKLSGWNMDSTFIDPMCGSGTIVIEAALMAYGIA
                     PGVFRRQFGFENWLDFDEKLLQSIYNDDSMEREFTHPIIGRDISRQTVESALLNVKHA
                     GLQKKVDIEVGSIFDFQPPADSQGIVVTNPPYGERLRREQIETFYKQLGDCFKQKYEG
                     YNVWMISSNMDALKNFGLRPSKRYTLYNGALECKFQRYSMFAGSVKAKYQGKGERIKS
                     KDRE"
     gene            complement(1232..2809)
                     /locus_tag="PK839_09255"
     CDS             complement(1232..2809)
                     /locus_tag="PK839_09255"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOG72962.1"
                     /translation="MEALDEFKELGLSEKALAAIKAKGFEQPSPIQKITIPVLLKETT
                     DVIAQAQTGTGKTAAFGLPIIEMFEGQPAGIKALILVPTRELALQVCDEIISLAAKRI
                     EVVAVYGGQSISEQQRRIRRGVDVVVGTPGRILDLIARKDLNFQNLKIFVLDEADEML
                     NMGFIEDIEAIMEHTPKEKRTLLFSATMPDRIVKLAKKYMKSAVKLEVDKAQPTTGLT
                     DQIYFEVRDSDKFDALTRIIDIEPEFYGLIFCRTRNDVDSLTSKLIERGYAAEGLHGE
                     ITQSQREKTLLKFRRRLLNILVATDVAARGIDIIDLTHVINYNMPQDADSYIHRIGRT
                     GRAGKQGTAITFISHDEYRKLNYVQKATKSNIRKEILPAAEDIVAVKKEKIKDELHEI
                     IKQGSYNDYLGMAEDILETNSPEVALAALLRLAYKSQLDVSNYSEARTFRVDTKGTTR
                     LFIGLGRNDGMTPKKISSFIRQEVQIPDNKIDDIVVRDDFSFITVPFVDAEKILKAFA
                     NQETDGKPLITKAKPKK"
     gene            complement(2880..4475)
                     /locus_tag="PK839_09260"
     CDS             complement(2880..4475)
                     /locus_tag="PK839_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007565810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOG72963.1"
                     /translation="MTKKLLYLCAFALIMPFGALSQNSSSFGAYKFVRFIQYLSGDYV
                     DTINVNKLVDNAIVEILSQLDPHSIYISKEDVQAMNEPLEGNFEGIGVEFNILNDTLM
                     VVNPISGGPSERVGILAGDRIIAIDGKSITNINLKITDVHKLLRGPKGTKVTVLIHRK
                     SLSTPVEFTITRDRIPIYSIDASYMAEQGIGYIKINRFAATTEQEFVEAMNKLNQQKM
                     NDLIIDLRNNGGGLLNSAYDLANYFFDPGKLIVYTEGRNSPRTNYFSTSKNKVFRGRV
                     VVLIDEGSASASEILAGAFQDWDRGIIVGRRSFGKGLVQNQLPLPDGSMIRLTVARYF
                     TPTGRAIQRQYKFGDLKDYYSDMTQRYDRGELFNKDSIHFADSLTYKTLIRHKTVYGG
                     GGIMPDVFIPLDTNYYSDYYGKLVRSGVINQFSLTWVDTHRNELLKEYPKFDKFKKNF
                     QVTEKMISELVALAESSGIPSDEKGLAQSANELKIQLKGLVAQSIFSTSHYFEVTNET
                     NETYQKALEIFHSWNDYKHLVQE"
     gene            complement(4540..6069)
                     /gene="guaA"
                     /locus_tag="PK839_09265"
     CDS             complement(4540..6069)
                     /gene="guaA"
                     /locus_tag="PK839_09265"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009275897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HOG72964.1"
                     /translation="MYEKIVILDFGSQYTQLIARRIRELNVYCEIYPYNQIPVLDGTI
                     KGVILSGSPFSVNDNASPKPDLSGIRGKLPILGICYGAQLLAASSGGQVMPSKSREYG
                     RATLEVMDVSSSLFKDFPIQSQVWMSHGDTITALPRNFLITASTTDIQVGAFQVTNEQ
                     TYGIQFHPEVYHTTEGITLLRNFVVDICGCSQTWTPASFIEETTRNLRETLGSDKVVL
                     GLSGGVDSSVAALLLHRAIGKNLTCIFVDNGLLRKNEYQQVLDSFGSMGLNIVGVDAS
                     QQFYNELKGVTDPESKRKIIGRNFIEVFEREAQKIQGVSWLAQGTIYPDVIESVSVNG
                     PSAKIKSHHNVGGLPNQMNLKVVEPLRLLFKDEVRRVGKELGLSAAILNRHPFPGPGL
                     GIRVIGEVTPQRVKLLQEADNIYIKALRESELYDKIWQAGAILLPVQSVGVMGDERTY
                     ENVVALRAVTSTDGMTADWYPFPHNFLSRVSNEIINKVRGINRVVYDISSKPPATIEW
                     E"
     gene            complement(6173..7099)
                     /locus_tag="PK839_09270"
     CDS             complement(6173..7099)
                     /locus_tag="PK839_09270"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042779 - tRNA 3'-trailer cleavage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HOG72965.1"
                     /translation="MTFSVTILGSSSAIPTPTRFPSAHVLNVHERLFLVDCAEGTQMQ
                     LRRFHIRMNRINHIFITHLHGDHVLGLFGLISTFNLLGRKQPLHLFAPKELESVLNFN
                     VGVFTDKLSFPVIFHPVDPNMSQLVFEDRHVSVTSLPLRHKVPTVGYLFKETPSLPNI
                     RKDCIQQYSLGLADIVKIKNGADYIAPDGKIIPNAELTFHARLPRSFAYCSDTLYSER
                     LVGLIRGVDLLYHEATFLDADVDLARQTGHSTALQAATAAVGAHVGKLIIGHFSSRYK
                     NISQHLQEARNIFPETYAAEDGQVYAIPEKFG"
     gene            complement(7096..7461)
                     /locus_tag="PK839_09275"
     CDS             complement(7096..7461)
                     /locus_tag="PK839_09275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOG72966.1"
                     /translation="MEFKIEKFDKFTLVEVLEEKLDTNIAPGLKSELVLISGNGERNL
                     ILDLSTCRYCDSSGLSAILVANRLCKNAGGTFVLTGLTDAVERLITISQLDTVLNIAY
                     SMEEAVQIIENAEMESSKK"
     gene            complement(7494..9461)
                     /gene="rpsA"
                     /locus_tag="PK839_09280"
     CDS             complement(7494..9461)
                     /gene="rpsA"
                     /locus_tag="PK839_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HOG72967.1"
                     /translation="MAKEATELEKPTPVAGENVNTVNLENFDWDLYAREGAYTPEQRK
                     ELEDIYDKTLSTVAENEVVEGTVVAITKREVFVNVGYKSEGVLSISEFRYNPDLKVGD
                     KIEVYVESSEDKRGQLVLSHKNARVLRSWDRVNAALEKDEIIKGYIKCRTKGGMIVDV
                     FGIEAFLPGSQIDVKPIRDYDIYVGKTMEFKVVKINQEYKNVVVSHKALIEAELEQQK
                     KEIISKLEKGQVLEGTVKNITSYGVFIDLGGVDGLIHITDLSWGRVNHPEEVVQLDQK
                     LNVVILDFDDAKKRIALGLKQLTPHPWDSLDTNLKVGDVVKGKVVVIADYGAFVEIAP
                     GVEGLIHVSEMSWSQHLRSAQEFLKVGDIVEAQILTLDREERKMSLGMKQLKPDPWDK
                     IDEKYPLGSKHTAKVRNFTSFGIFVEIEEGVDGLIHISDLSWTKKIKHPAEFTSVGAE
                     IEVVVLEIDKENRRLSLGHKQLEENPWDVFETIFTVDSIHEGTVIDVFDKGAVVALPY
                     GVEGFVTPKHLVKEDGSSAKVDEKLPFRVLEFNKAAKRIILSHSRTYEEDKKAEEKAD
                     KKSKTSSTQKAVKKLNANIEKTTLGDISELAALKTELEENSKKEAKAKKETKPKKETK
                     TAKAAASEDENVKVDEPKADAEAKDEPKVDE"
     gene            9907..10857
                     /locus_tag="PK839_09285"
     CDS             9907..10857
                     /locus_tag="PK839_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329363.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HOG72968.1"
                     /translation="MSDVYPWGDNRRFNTYSGYFKREFGGRVQKLAIDAGFSCPNRDG
                     TVATGGCTYCNNEAFNPSYCHPTKSITQQIEEGIEFHKVRYRRAKRFLAYFQAYSNTH
                     APIEHLRMLYEEALAFPGVIGLVIGTRPDCVDEEKLNYIGQLAAKHYVALEYGVESCY
                     NRTLEAINRGHTFEQSVWAIEETHKRGIKVGAHIILGLPGETRNEMLAEASILSSLPI
                     HTVKFHQLQVIRGTVMAKRYELDPGAFQLFGMEEYLNFMVDVVERLNPNMVVERITGE
                     APPRFLATNGWGSYRTDQLMVMFEKLLEQRNTWQGRLYKG"
BASE COUNT         2867 a         2567 c         2440 g         3016 t
ORIGIN      
        1 tccgccggta ggcggacaag tttatcttat tctctatcct tactctttat cctttcccct
       61 ttaccctggt acttcgcctt tacactaccg gcaaacatgg agtagcgttg gaacttacat
      121 tcaagagcgc cgttatagag ggtgtagcgt tttgatggtc gcaaaccaaa atttttcagg
      181 gcatccatgt tcgagctgat catccaaacg ttgtacccct catatttctg tttaaagcag
      241 tcgcccagct gcttgtagaa agtttcgatt tgctcacgtc gaagcctttc gccgtaaggt
      301 ggattggtaa caacaatgcc ctgagaatcg gcaggaggct gaaagtcgaa gatggagcct
      361 acttcaatgt caactttttt ctgcaaacct gcatgcttaa cgttgagcaa cgccgattca
      421 acggtctggc gcgagatatc acgtccaata attggatgtg taaactcccg ctccatgcta
      481 tcgtcgttat agattgactg gagaagcttc tcatcaaaat cgagccagtt ttcaaaacca
      541 aactgccggc gaaaaacacc gggtgctatt ccatacgcca taagggctgc ctcaataaca
      601 atagttccag agccgcacat tgggtcaata aaggttgaat ccatattcca acccgaaagt
      661 ttgattagcc ctgctgccag cacctcgttg ataggggcaa tatcctgagc agttcggtat
      721 ccgcgacggt gcaacgattc ccctgaggaa tcgagcagga tggtgcagct atcgcccgaa
      781 atgtggacgt ggaactgtat ttggggattt ttggtatcaa caaacggacg ttttccgtag
      841 cgatcgcgga actgatcaac cacagcatct ttaaatttaa gggcaatgta acgtgaatgt
      901 gggaaaacct cagagttgac cacgctgtca actgcaaatt tttgcgataa gtccatcagt
      961 gaagtccagt cgaacttacg ggcatttccg tagaactcct caatggttcg ggcgctaaat
     1021 gagtaaacag gcttaagcac ccgaatggct gttcgtaacc agaagttagc cttgtaaagg
     1081 gtttccgttg ttccccggaa gctcacggcc ctattttcga tcttcacatc ctcagcacca
     1141 agttcggtga gttcatcggc aagaacaccc tccagcccct ggagggtttt ggcaatcatt
     1201 tcaaactttt ccaccataaa ggcctactcg cttatttttt tggtttggct tttgttataa
     1261 ggggtttacc atctgtttcc tgattggcaa aggccttcag aattttttcg gcatccacaa
     1321 agggcacggt aataaaggaa aaatcatcgc gaacaacaat gtcgtcaatc ttattatcgg
     1381 ggatttgaac ctcctgcctg atgaaggaag aaatcttttt tggggtcatg ccatcattac
     1441 gaccaagtcc aataaacaag cgggttgtgc ctttagtatc caccctaaaa gtacgagcct
     1501 cggagtagtt ggagacatcg agttgcgatt tgtaggctag gcgcaacagg gcagccaacg
     1561 ccacctcggg gctattagtt tcaaggatgt cttcggccat tccaaggtag tcgttatatg
     1621 agccctgctt gataatttcg tggagttcgt ccttaatttt ttccttttta acggcaacga
     1681 tatcctcggc agcaggtaga atttccttac ggatattcga tttggtggcc ttttgaacat
     1741 agttcaactt gcggtactca tcgtgagaaa taaaggtaat ggccgtgccc tgcttgccag
     1801 ctcttccggt tcgtccaatc ctgtgaatat aggagtcggc atcctgaggc atgttgtagt
     1861 tgataacgtg ggtaaggtcg ataatgtcaa ttcctctggc agccacatcg gttgccacca
     1921 ggatgttcag caaacgtctc ctgaatttta aaagggtttt ttcacgctgg ctttgggtga
     1981 tctcgccatg caacccctcg gcggcatagc cacgctcaat aagcttggag gtgagcgagt
     2041 caacatcgtt acgggtacgg cagaatatca ggccgtagaa ttcaggttcg atgtctatga
     2101 tgcgggttag cgcatcaaac ttatcggaat ctctaacctc gaagtaaatt tgatcggtaa
     2161 ggccagtagt tggctgtgcc ttgtccacct cgagcttaac agccgacttc atgtacttct
     2221 tggccagctt aacaattcta tcgggcatgg tggccgagaa aagcagggtg cgtttttcct
     2281 ttggcgtatg ctccatgatg gcttcaatat cttctataaa acccatgttg agcatttcgt
     2341 cggcctcgtc gagaacgaaa attttcaggt tctgaaagtt aaggtccttg cgggcaatta
     2401 aatcaagaat tctacccggg gttcccacaa ccacatcaac acctcttcta atcctgcgct
     2461 gctgctcaga gattgattgg ccgccgtaaa ctgcaaccac ctctatacgt ttggcagcca
     2521 gcgaaattat ttcgtcgcaa acctgaaggg ctaactcccg ggttggtacc aaaataaggg
     2581 cttttatgcc tgcaggctgg ccctcgaaca tttctataat gggtagtcca aatgccgctg
     2641 tttttcctgt tcctgtttga gcctgggcta taacatcggt tgtctccttt aaaagtaccg
     2701 gtatggtaat tttttgaatt ggcgaaggct gctcgaaacc cttagctttt attgctgcca
     2761 atgccttttc ggataacccg agctctttaa attcatcaag tgcttccatt aatgtaatga
     2821 taagttggac tcattcatag agtccagtta aattattagt aaaattaatt aaatttatgt
     2881 tattcctgta ctaaatgctt gtagtcgttc cagctatgaa atatttccaa tgctttttga
     2941 taagtttcat tggtttcatt ggttacctca aagtagtggc tggtagagaa aattgactgg
     3001 gcaaccagcc ccttgagctg tatcttaagt tcattggccg actgggctaa acctttctca
     3061 tcgctgggaa ttccgctact ctcggctaaa gccaccagct cggagatcat cttttcagtt
     3121 acctgaaaat tctttttaaa cttgtcgaac ttggggtatt cttttaaaag ctcattcctg
     3181 tgggtatcta cccaggttaa ggaaaattgg ttgataactc ccgaacgaac cagcttaccg
     3241 tagtagtcgg aatagtagtt ggtgtcgagc ggaataaaca cgtcgggcat gatgcctccg
     3301 ccaccataaa ccgttttatg cctgatgagg gttttatagg taagcgaatc ggcaaaatga
     3361 atgctatcct tgttgaagag ttcgcccctg tcgtaacgct gagtcatatc ggaatagtag
     3421 tccttcagat caccaaattt atactggcgt tgaatggctc taccggttgg ggtgaagtaa
     3481 cgtgcaacgg tgagtcgaat catggaacca tcgggcaagg gcagctgatt ttgcaccaac
     3541 cccttaccaa acgatcgcct tccaacaata attcccctat cccagtcctg aaatgctccg
     3601 gcaagaatct cgcttgccga ggccgatccc tcgtcaatga gcactaccac ccttcccctg
     3661 aataccttat ttttagaagt ggaaaaatag ttggttcttg ggctattacg accctcggtg
     3721 taaacaatca gcttgccggg gtcgaaaaag tagttggcca ggtcgtaagc agagttaagc
     3781 agtcctccac cgttgtttct caggtctatg atcaggtcgt tcattttttg ctggttcaac
     3841 ttgttcatag cctcaacaaa ctcctgctcg gtggtagcgg caaatcggtt aatcttgatg
     3901 tagccgatgc cctgctcggc catgtaggag gcatcgatgc tatagattgg aatacggtcg
     3961 cgggtgatgg tgaactccac aggtgtacta aggctcttac gatgaatgag cacggttacc
     4021 ttggttccct ttgggcctcg cagcaactta tgcacatcgg tgattttcag gttgatattg
     4081 gttatagact tgccatcaat ggcaattatt ctgtcgccgg ctagtatgcc aaccctctca
     4141 gatggtccgc ccgaaatggg attaaccacc ataagggtat cgttcagaat gttgaactcc
     4201 acaccaatcc cctcgaaatt tccctcaagg ggctcgttca tagcctgaac atcctctttt
     4261 gaaatataga tggagtgcgg atcgagctgt gagagtattt caacaatggc attgtcaacc
     4321 aacttgttaa cgttgatagt atcaacgtaa tcgcccgata ggtactgaat aaaccgaaca
     4381 aacttgtagg ctccaaagct tgaagagttc tgcgatagtg ccccaaaagg cattatcagg
     4441 gcaaaagcac atagatagag tagtttttta gtcatggtaa aggaaaattt ctacatgtag
     4501 taaaacccga aacaaatggt aaaagttcat agagtaagcc tactcccatt caattgtggc
     4561 aggaggcttg gagctgatat cgtaaaccac cctgtttatc cctctcacct tgttaatgat
     4621 ttcgttagat accctggaca gaaaattatg gggaaatggg taccaatcgg cggtcattcc
     4681 gtcggttgaa gtaacagctc taagggcaac cacattctcg taggtacgtt cgtcgcccat
     4741 aactccaacc gactgcaccg gaagtagtat ggcacctgcc tgccatatct tatcgtatag
     4801 ttccgactcc ctcagggcct tgatgtatat gttgtccgcc tcctgaagca gcttaactct
     4861 ttggggggta acctcgccta tcaccctaat gcccagtcct gggcccggga agggatgcct
     4921 gttaagtatt gcagccgaaa gccccaactc cttaccaacc ctacgaactt cgtccttgaa
     4981 aagaagtcta aggggctcaa caaccttaag gttcatctgg tttggcaacc cgcccacgtt
     5041 gtggtgcgat tttattttgg ccgaagggcc attaaccgag accgactcta ttacatcggg
     5101 gtagatagtt ccctgagcga gccagctgac cccttgaatt ttttgagctt cacgctcaaa
     5161 gacctcaata aagttacggc caataatctt acgtttggat tcgggatcgg taacaccttt
     5221 tagctcgttg tagaactgct ggctggcatc aactccaact atgttaagcc ccatgctgcc
     5281 aaaagagtcg agcacctgct ggtactcatt tttacgaaga agcccgttgt caacaaaaat
     5341 gcaggttaga tttttcccaa ttgccctgtg caaaagcagg gcagcaactg aagagtcaac
     5401 cccacccgat agtcctaata ctaccttatc gctgcccaga gtttcccgaa gatttctggt
     5461 agtttcctca ataaacgatg caggtgtcca tgtttggctg caaccgcaaa tgtccaccac
     5521 aaaatttcgg agtagggtaa ttccctctgt agtatggtaa acctcaggat ggaactgaat
     5581 accataggtt tgctcgtttg ttacctgaaa tgcgccaacc tgaatatccg tggtgctggc
     5641 agtaatcaaa aagttacggg ggagcgcggt gatagtatcg ccatgcgaca tccaaacctg
     5701 cgactgtatg gggaaatctt tgaataagct agatgaaaca tccattactt caagtgtagc
     5761 gcgtccatac tccctacttt tagaaggcat cacctggccc ccgctgctgg cggcaagtag
     5821 ctgggctcca tagcaaattc cgagaattgg caatttcccc ctaattccgc tcaaatcggg
     5881 tttaggagag gcgttatcat tcacggaaaa tgggcttccc gaaaggatta ctcctttaat
     5941 tgtgccatcc aacaccggga tttggttgta ggggtatatt tcacagtaaa catttaactc
     6001 tcttattcgg cgggcgatta gctgcgtgta ctgtgagccg aaatcgagta taacgatttt
     6061 ttcgtacatt gaaaaatgac tattttcaga atttgcaaag gtataaaata aaccttaaat
     6121 gaactaggaa tatggtagtt ggggttaaaa ttctgttgta acaagcatcc ggttagccga
     6181 atttttcggg aatggcatat acttggccat cttcggcagc ataggtttca gggaaaatat
     6241 ttcttgcctc ctgcaggtgt tgcgaaatgt tcttgtaacg ggatgaaaaa tgtccaatga
     6301 ttagcttccc aacgtgcgcc ccaacggcag ctgttgcagc ttgcaaggca gtggagtgtc
     6361 ccgtttgacg ggccagatct acatctgcat ccagaaaggt agcctcatgg taaagtaaat
     6421 ccacacctct aatcagccca accagtctct cgctgtagag ggtatcggaa cagtatgcaa
     6481 atgagcgggg caaccgggca tggaaggtca actccgcatt tggaatgatt ttgccatcag
     6541 gagcgatata gtcagcgccg tttttaattt ttacaatatc ggccaagccc agtgagtatt
     6601 gctgaatgca gtcctttcgt atgttgggta gcgagggggt ttccttaaaa agataaccca
     6661 cggtaggaac tttatgccta aggggtagcg aggtaacgct gacatgccta tcctcaaaaa
     6721 ccagctgcga catgttgggg tcaaccggat ggaaaataac agggaacgag agtttatcgg
     6781 taaaaacacc cacgttgaag tttagcacgc tttccagctc cttaggggca aacagatgca
     6841 ggggctgctt ccgacctaat aggttaaagg ttgaaatcag gccgaaaagg cccagaacgt
     6901 gatcgccatg aaggtgggtg atgaaaatat ggttaatacg attcatccgg atatgaaagc
     6961 ggcggagctg catctgtgtg ccctcggcgc aatcaactaa aaacaaccgc tcatgcacgt
     7021 tgagcacatg agcggatgga aatcgggttg gtgtagggat agccgagcta cttcccaaga
     7081 tggttacaga aaaggtcact tcttgctgct ctccatttct gcattctcta taatctgcac
     7141 ggcttcctcc atggagtagg caatgttgag caccgtatcg agttgggata tggtaattag
     7201 cctttcaacc gcatcagtca aaccggtgag cacaaaggtg ccacccgcat tcttgcaaag
     7261 cctgttagca acaagtatgg cacttaatcc tgatgaatcg cagtaacgac aggtactcag
     7321 gtcgagtatc agattacgct caccattccc cgatattaaa accaactctg attttaaccc
     7381 tggggcgatg ttggtgtcaa gtttttcctc caacacctca accagcgtaa acttatcaaa
     7441 tttctcaatt ttaaattcca tcgttttcag gattaaatgg ttatactttg ccgctactca
     7501 tcaactttgg gttcgtcctt tgcttcggca tcggccttgg gttcgtccac ttttacgttt
     7561 tcatcctcac tcgcagctgc tttagctgtc ttggtctcct ttttaggctt ggtttccttc
     7621 ttggcctttg cttccttttt gctgttttcc tcaagttcgg tctttaaagc tgccagctcg
     7681 gaaatatccc ctaaggtagt tttttctata ttagcattca actttttaac agctttttgt
     7741 gtggaagaag ttttagattt tttatccgcc ttttcctccg ctttcttatc ctcctcatag
     7801 gttcgactat gggagagaat tatgcgttta gctgccttgt tgaattcgag caccctgaag
     7861 ggaagcttct cgtcaacttt agctgaggag ccgtcttcct taaccagatg cttaggggta
     7921 acaaatcctt caaccccata gggtaaggcc acaacggcgc ccttgtcgaa tacatcgatg
     7981 acggtgccct catggatgga gtcaacggtg aaaatggttt cgaaaacatc ccatgggttt
     8041 tcctccagct gcttgtggcc caagctgagg cgacggtttt ccttgtcaat ttcaagaaca
     8101 actacctcta tttctgcacc aaccgaggta aactcggcag ggtgtttaat tttctttgtc
     8161 cagcttaggt cggagatatg gataagccca tcaacccctt cctcaatttc tacaaaaatc
     8221 ccaaaggagg taaaattcct aacctttgcg gtatgcttag aacccaaagg atacttttcg
     8281 tcaatcttat cccatgggtc gggtttaagc tgcttcattc ccagcgacat cttgcgctcc
     8341 tcacgatcga gggtgagtat ttgagcttca actatatcgc ccaccttgag aaactcctga
     8401 gcgctacgca ggtgctggct ccacgacatt tcggaaacat gaataaggcc ttcaacgccg
     8461 ggggcaattt caacaaaagc cccgtaatcg gcaataacca caaccttacc tttaaccaca
     8521 tcgccaacct tgaggttagt atcgagcgaa tcccaaggat ggggtgtaag ctgcttcagc
     8581 ccaagggcaa tacgcttttt ggcatcatca aaatcgagga ttaccacgtt aagtttttgg
     8641 tcgagctgaa caacctcctc ggggtggttg acccggcccc aggaaaggtc ggtaatatgg
     8701 atgagcccat caacgcctcc caaatcaatg aatacaccgt agctggtaat atttttaacg
     8761 gtgccctcaa gcacctgacc tttttcaagt ttggagatga tttccttctt ttgctgttcg
     8821 agttcggcct cgataagcgc cttatgcgac accacaacat ttttatactc ctggttgatt
     8881 ttaacaacct tgaactccat ggtttttccc acgtagatgt cgtagtcgcg tattggcttc
     8941 acatcaattt gagagccggg caggaatgcc tcaataccga aaacatccac tatcataccg
     9001 cccttagttc tgcattttat atatccctta attatctcat ccttctccag ggctgcatta
     9061 acacgatccc atgagcgtag aacacgggca tttttatgcg aaagaaccag ctgtccgcgt
     9121 ttgtcctctg aactttcaac ataaacttca atcttatcgc ctacctttaa atcgggatta
     9181 taacgaaact cgctgatact cagcactccc tccgacttgt agccgacatt aacaaatacc
     9241 tcacgcttgg taattgctac gactgttccc tcaactacct cattttcagc aactgttgat
     9301 agagttttgt cataaatatc ctccagctcc tttcgttgct cgggggtgta ggccccttcc
     9361 ctggcgtaca aatcccagtc aaaattttct aaattcacag tgttaacatt ctctcctgcc
     9421 acaggggtag gtttttctaa ttctgtagcc tctttggcca tttcttcctg atttgtcatt
     9481 taaacgattt acaattaatt agttagacat tatgtatggg aattaatcaa actgcaaaaa
     9541 tatataactt taacatttat aggaaaaaat accatttttt tctaaaaagt atctgcaata
     9601 tagctgaaat tcataaagat aaaaaattat tttaacagct aaaaaacaaa ttacctgcat
     9661 aggtagccag atattgccaa caaagaacca caaatcagaa ttttgaagtg ctcaacaaga
     9721 tcaagaagta aaagttatag ctacccaagc aaaaatgcaa taccaacttt tacgaatata
     9781 cagttcattg aaatcgctac ctttgcacat cataaacagc catctgagat agctgatttt
     9841 gctataggcc aatatcagaa aggctctacc agtacactta caccaaaaaa acaagcgtgc
     9901 acgtagatga gcgacgttta cccatggggc gataaccgcc gattcaacac ctactcgggt
     9961 tactttaaac gggagtttgg tggtagagtt caaaagttgg ccattgatgc cggtttttcg
    10021 tgtccgaacc gtgacggtac agttgcaacc ggtggatgca cctactgtaa taatgaggcg
    10081 tttaacccca gctactgcca ccccacaaaa tcaattaccc agcaaattga ggaagggatt
    10141 gagtttcata aggttcgcta tcgtcgggca aaaagatttc tggcatactt tcaagcctac
    10201 tccaataccc atgcaccaat agagcacctt cgcatgcttt acgaagaagc gctggccttt
    10261 cccggggtta tagggctggt tattggaact cgccccgatt gtgttgatga agaaaaactc
    10321 aactacatcg gacagcttgc agctaagcat tacgtagctc ttgagtacgg tgtggaatcg
    10381 tgctacaacc gaacgcttga ggcgattaac cgggggcata ccttcgagca atcggtttgg
    10441 gctatcgagg aaacgcataa gcggggaata aaggttggtg ctcatataat tcttgggcta
    10501 ccaggcgaga cgcgcaacga aatgctggct gaagccagca ttctatcctc ccttcctatt
    10561 cacaccgtga agttccatca acttcaggta atcaggggga cagtcatggc aaaacggtat
    10621 gaattagatc ctggggcatt ccaacttttt gggatggagg agtacctcaa ctttatggtt
    10681 gacgtggtag aaaggttaaa ccccaacatg gtggtggagc ggataacggg agaagctccg
    10741 ccccgattcc tggccactaa tggttggggt agctacagaa ccgatcagct tatggtcatg
    10801 ttcgagaagc tactcgaaca gaggaatacc tggcagggaa ggttgtataa aggataaagg
    10861 gtaaaggata aaaaaattca agattcaaaa
//
LOCUS       DAOSHM010000073        10798 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_5140_length_10798_cov_21.047100, whole genome shotgun
            sequence.
ACCESSION   DAOSHM010000073 DAOSHM010000000
VERSION     DAOSHM010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 10798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10798
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5140_length_10798_cov_21.047100"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..784)
                     /locus_tag="PK839_09290"
     CDS             complement(<1..784)
                     /locus_tag="PK839_09290"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOG72969.1"
                     /translation="MAEKLEYIIKNGTVVTSTNTQKVDILIKNGKIAALANEFNDDMF
                     QTINANGMYILPGGVDPHVHLHLPTPAGYSSDDFHSGSIAALYGGTTTIIDFVTPHRG
                     QPLTEALAARIEEANECLTDYSFHVSPVDWRRESEDEIKRCIDLGFPSFKIYMAYLDT
                     NGLHNTDIQRVMGVVGRNGGMVTAHCELGEEVDRLRDSFYRQGKTQPLYHQLSRPPQV
                     EVKAVERAIAMAQQANCPLYVVHLSAGSSLEPVHRARAAGQKV"
     gene            complement(777..1415)
                     /locus_tag="PK839_09295"
     CDS             complement(777..1415)
                     /locus_tag="PK839_09295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72970.1"
                     /translation="MNADILEWIGYTASVIIALSMALNSIVKFRWVNLTGALLFSIYG
                     FLIGALPVGILNGIIVVTDLYYLIRIYSQKDTFEILEIRPDNKYLLRFLDYHSKDIQK
                     YFPNFSYSPDENTISFFVLRNMVVAGVFVARKVADRTLEVQLDYVLPEYRDFKNGRFV
                     YSWLVQNFTEHGFARVWAKAETEKHRNYLLKVGFNITSDGHFYKTLNENQHG"
     gene            complement(1471..2745)
                     /locus_tag="PK839_09300"
     CDS             complement(1471..2745)
                     /locus_tag="PK839_09300"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOG72971.1"
                     /translation="MAILLKNATYIEWQTLEFRKTNILVKPGKVDFYDPANIPTGVDE
                     VIDCTGKLVTKSFVVGHHHAYSALSRGMNPPRKTPTNFYEILKYVWWTLDKCLSTEMV
                     EYSGLVTAMACAKAGATFAIDHHASPNAVSNSLSTLASAFEKVGVNHLLCYEISDRDG
                     ELIAQQGLEETDRYLDKHQGLVGLHASFTLGDKTLTSAAELMRRHQSGVHIHVAEGIY
                     DQEHCHQNYGQSVIERLNSFGLLSSSKSILVHCLHLSGNERDIIASSPCWVVQNTDSN
                     LNNNVGYFNSKGLGNNLMLGTDGMHSDMLQSMKSAYFVGQNFDPMSPMQAYSRLRNAH
                     RYLSSNNFSGDDESNLLVIDYNSPTPVTPDNFAAHLVFGINSSHIRDVISNGKLIVKN
                     HRLTLVDEEALLIESRKIAAYLWKRMGDVGAE"
     gene            complement(3148..5715)
                     /gene="xdh"
                     /locus_tag="PK839_09305"
     CDS             complement(3148..5715)
                     /gene="xdh"
                     /locus_tag="PK839_09305"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0046415 - urate metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent xanthine dehydrogenase"
                     /protein_id="HOG72972.1"
                     /translation="MITFTLNSKIVEFTGDPKSNLLVFLRNTMHLTAAKDGCSGQGVC
                     GACSVEVDGKIKMACRTKMGDIEGSVVFTLEGLDETFRKTLGRNFALRGAVQCGFCSP
                     GMIMRAKGLYNQNPKPSRNEIIRAITPNLCRCTGYVKIIDAIDASFKELAGELEPTVV
                     AEAKIGERYPKYQAEATALGERPFVDDLRIDGMLFGALKFSDHPRAEILSIDVAPAMS
                     IDGVVGIYTAKDIPGNHFSGLVFQDWPLMVGKGETTHYIGDVVASVVATTEETARKAV
                     EQIRVTYRVFSPVTDIHQAILPNSPNVHTERSNVLETCTIRFGEVKKAFEEAAYISSD
                     VYETQRIEHAFLETETALALPDGDGVKLYSQGQGVYIDRSQIAKFLDIPEEKVKVVQV
                     QNGGGFGGKEDLTVQGHAALCAYLSGMPIKIHLNREESLRMHPKRHPVWMKMSLACNS
                     KGKFTALKLDAIGDTGAYASVGTKVMERVVGHATGGYTIPAVDISAKTVYTNNIPSGA
                     MRGFGVPQVTFALESCIDDICRQAGFDRWQIRFDNALENGAKTATGQILSGVGLKKCL
                     LAVKDDFISARFAGIAAGLKNTGVGNGMVDDSEVLIKIISGKRVEIHHGWSEMGQGVH
                     TMAIQTLHQETGIDPSIVEVIVNTEAGVPTGMTTSSRATALVANALINASAALKADLA
                     EASLEQLAGRTYRGKFVCDWTSKPGAETENPVVHFAYGYAAQVAILDDEGRLRKLVAA
                     HDAGKIMNRMLFEGQIEGALHMGMGYALTEDFPMSEGYPTSYKFNDIGIIRANEMPEL
                     KVIGIEEADPVGPYGAKGIGEIGLVPTAAAIANALMAYDGIKRTKLPMRRSRIKDKE"
     gene            complement(5851..7089)
                     /gene="thrC"
                     /locus_tag="PK839_09310"
     CDS             complement(5851..7089)
                     /gene="thrC"
                     /locus_tag="PK839_09310"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HOG72973.1"
                     /translation="MNSAKYIYRCCCCGKEYGGSSDIYLCPSCSKLNQPENPPKGVLK
                     VIYDYDSICQNIAQLAGNGYIDLLPIRSLSSLPQLKVGKTPLYKYSRMEGKNLPFTLL
                     LKDDSQNPTFSFKDRASALVSAYALENGKNEIVTASTGNAGSSLAGICASQGQKAIIM
                     VPKNAPAAKLTQIVMYGASIVPVDGSYDQAFDLSVKSSETFGWYNRNTAFNPLTIEGK
                     KTVSFEIFEQLKGQLPDRIFVPVGDGVIISGVYKGFEDLLMLKLIDRMPTIVAVQAKG
                     SSNLVGNLFTDVFCSQTSQTLADSISVDIPRNFYMARQYIKTYNGEAVTVTDGEILEA
                     SAILARNTGLFAEPAASAALAGLISFFRQNKVEGNTSNMVLLTGSGLKDLKAVSPIVQ
                     FPKAIKPKIDALKELVDTWK"
     gene            complement(7124..8608)
                     /locus_tag="PK839_09315"
     CDS             complement(7124..8608)
                     /locus_tag="PK839_09315"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HOG72974.1"
                     /translation="MIKIVDCVSNAKALENAVGRFREKGIILPTIKQQQNPELVPSKI
                     QEQLRGIGLWEINPLNLFRITWKNEPKEKGGLFGGVNFIELPSELTGVKARIVMLIGK
                     WFPTGAHKVGAAYGCLAPQITTGGFDPTTQKAVWPSTGNYCRGGAFDSKLMGADSVAI
                     LPQEMSSERFTWLRDYVGSEVIATPGCESNVKEIYDKCWEIRRTKPNYIIFNQFDEFG
                     NSAWHYNVTGKAIEEVFSNLKGGQKNLAAFVSATGSAGTIAAGDYLRTIAPHVKVVAS
                     EALQCPTLYCNGFGGHRIEGIGDKHVPWIHNVKNTDVVTAIDDEDCMRLFRLFNEPEG
                     KSYLASLGIPIETVNMLNLLGISGISNLLSAIKTARYFEMTSDDIIVTIATDSAEMYL
                     SRLEELSNERGAYSQIQAIKDYEKCLLGVSTDWIKELTYTDRKAIHNLKYYTWVEQQG
                     KNVEDLNQLWYDRNIWQNLFNQVSRWDELIEEFNDRTGLLKSLR"
     gene            8875..9336
                     /locus_tag="PK839_09320"
     CDS             8875..9336
                     /locus_tag="PK839_09320"
                     /inference="COORDINATES: protein motif:HMM:NF013224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HOG72975.1"
                     /translation="MKNIDEIDGKLLNILQDNSRVTIRELSEKLHLSTTPIHERIKKL
                     EKSGIIKQYVTLLDPKILGKKLTVFISVSLSSHVKEVIDEFEREIKVMPEVMECYYVS
                     GTWDFFIKIQCTDMDDYHNFVINRFSRLKNITQFYSSFVMSESKVSYKHQL"
     gene            complement(9337..10437)
                     /locus_tag="PK839_09325"
     CDS             complement(9337..10437)
                     /locus_tag="PK839_09325"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOG72976.1"
                     /translation="MKILQVVTHFDFGGAENHVRELSNELAAQAHDVYLVGPSGKQIK
                     LLNQKVHFVQASQLSKLLVFQTFFLIYLIKKHQIEVIHAHQRLSILAACWAGFITGTP
                     VVATVHGRVRFDLRSSFSRTRTSKIIFVSHQVLKVSKYYSEINHKSVVIPNGINFQKV
                     IPKRIPYGMAYISRMDSHHKEVVDMLIGLMPDVTASFPQASLWIFGEGKQVEYLKKVA
                     SQLNQSLHREVVRICGYVENFNQQEILPELVLGVGRVAVESLANGLNVISVNCKRMGS
                     IVNLRNYSAYRLNNFVDINGIPPTRESLLSEINKFFQNRVQYKDESILIREKVIQEFN
                     IKTITLQTMNVYSEATQKQLQKNVTSSSSHTC"
BASE COUNT         2850 a         2433 c         2363 g         3152 t
ORIGIN      
        1 aaaccttctg tccggcagct cttgcacgat gaacaggctc aagagagctg cccgcagaca
       61 ggtgaacaac gtagagggga caatttgcct gttgtgccat ggcaatggca cgttcaacag
      121 ccttaacctc cacctgcggt ggacgtgaaa gttggtggta gagaggctga gttttgccct
      181 gccggtaaaa ggagtcacgc agcctgtcaa cctcttcgcc aagctcgcag tgagcggtaa
      241 ccattcctcc attccttccg actacgccca ttacccgttg aatatccgta ttgtgaaggc
      301 cgttggtatc aaggtaagcc atgtagattt taaaggatgg aaatcctaaa tcaatgcacc
      361 gcttaatttc atcctcactc tccctgcgcc agtcaaccgg actaacatga aaggagtaat
      421 ccgtgagaca ctcattagct tcttcaattc ttgcagctaa cgcctcagta aggggttgcc
      481 ccctatgcgg ggtaacaaaa tcaataatag tggttgtccc tccgtatagg gcagctatgc
      541 tacccgaatg aaaatcgtcc gatgagtagc cagcaggagt tggaagatgc agatggacgt
      601 gcgggtcaac acctcctgga agtatgtaca taccattggc attaatggtc tggaacatat
      661 cgtcattaaa ctcgttggcc aatgctgcaa ttttcccgtt tttaataaga atatcaactt
      721 tttgggtatt agtagaagta acaaccgttc catttttaat aatgtattcc aatttttcag
      781 ccatgttggt tctcgtttaa agttttgtaa aaatggccat cgctggtaat gttaaaaccc
      841 accttaagta ggtagtttct gtgcttttcg gtttcagcct tagcccaaac cctggcaaaa
      901 ccatgctcgg tgaagttttg cactagccac gagtaaacaa accttccatt tttaaaatcc
      961 ctatattcgg gcaaaacgta gtcaagctga acctccaggg ttctatcagc gaccttccgg
     1021 gcaacaaaca ccccagccac taccatgtta cgaagcacaa agaagctaat ggtgttctca
     1081 tcgggggagt aggaaaagtt gggaaagtat ttctgaatat cctttgagtg gtagtctaga
     1141 aatcgcagta ggtacttgtt gtcgggtctt atttcgagaa tctcgaatgt atccttttgg
     1201 gagtaaattc ttataaggta gtaaaggtcg gtaacaacaa taattccatt gagaataccc
     1261 actgggagtg cacctattag aaagccatat attgagaaaa gtaatgcacc ggtgaggttt
     1321 acccagcgga acttgacaat ggagttgagc gccattgaaa gggcaataat caccgaagcc
     1381 gtgtagccaa tccattccaa aatatcagca ttcataggag gtagaatttg tttggcaaag
     1441 gtaggcaatt aatacgtagt gtcagcaaat tcattcagct cctacatcgc ccatacgttt
     1501 ccataggtag gctgcaatct ttctggattc gattagtaag gcctcctcgt caaccagtgt
     1561 taatcggtgg tttttaacaa taagcttacc gtttgaaatt acatcccgta tgtgcgagga
     1621 gttaataccg aaaaccaggt gggcagcaaa attgtcaggg gtaaccgggg tgggggagtt
     1681 gtagtcaatt accaacaggt tcgactcgtc atcgccggaa aagttgttgg aggaaagata
     1741 cctatgagcg tttctcaacc ggctgtaggc ctgcatgggg ctcatggggt cgaagttttg
     1801 ccctacgaag taggccgatt tcatactctg cagcatgtcg ctgtgcattc cgtcggttcc
     1861 cagcatgagg ttattgccca ggccctttga gttgaagtag cctacattgt tgttcaggtt
     1921 gctatcggtg ttctgcacca cccagcatgg ggagctggca attatatctc gttcgttacc
     1981 tgagaggtgg aggcagtgaa ctagaataga cttggaggag cttagcaacc cgaagctatt
     2041 tagcctttcg attaccgatt ggccataatt ctggtggcaa tgctcctgat cgtatatacc
     2101 ttctgccaca tgaatatgaa ctccactttg atgcctgcgc atcagctctg cggcagaggt
     2161 aagtgtttta tcacccagtg taaacgaggc atgtagtccc accaatccct ggtgtttgtc
     2221 caggtagcgg tcggtctctt ccaatccctg ctgggcgatt agctctccat cacgatcgct
     2281 tatttcgtag cataacagat ggttgacgcc aaccttttca aatgcgctgg caagcgtact
     2341 aagcgaattg ctcacagcat taggtgaggc atggtggtca atggcaaatg ttgcgccagc
     2401 tttggcgcac gccatggctg taacaagtcc gctatactca accatttcgg ttgacaggca
     2461 tttatcgagg gtccaccaaa catactttaa aatttcgtaa aagttggtgg gcgtttttcg
     2521 gggtggattc atcccgcgcg atagggcgga gtatgcatgg tggtgcccca caacaaacga
     2581 cttggtgacc aactttcccg tacagtctat tacctcatca accccagtag gaatgtttgc
     2641 cggatcatag aaatcaactt tccctggctt taccaaaatg ttagttttcc tgaattcaag
     2701 cgtttgccac tcaatgtatg ttgcgttctt tagcagtatg gccatcgttt atgtaatttt
     2761 tatgtgtttc aaaacttata atccatgaga agctataaaa gcctgtcctg agcagctcgg
     2821 caggtgttat agtgagtagt tcgactggct cactataaca ccagccgaac tactcgcttt
     2881 aacacctgtt gtagggtaaa cccttttagc cgaaggtttc ttttaccctt tatctctttg
     2941 gctctattaa aaaaataatg ttgttatacc gattatcact ctatatgata agtttgttaa
     3001 acttttcttt gtgaactttg tggttaaata atactagaaa ccacaaagca caaaaagata
     3061 ttacagaacg caaagattac tattcatgat ttcgttatgg ttatcgggga aaaacatttt
     3121 ctttaaattg agccattttt ttattcttta ttctttatcc tttatcctgg accttctcat
     3181 gggtagcttt gtcctcttta tcccatcgta ggccattaat gcatttgcaa tagctgcagc
     3241 ggtgggtact aagccaattt caccaattcc ttttgcacca taagggccaa caggatcggc
     3301 ttcctcaatt cctatgacct ttaactcggg catttcgtta gctcttatga ttccaatatc
     3361 gttgaatttg tagctggtag gatagccttc gctcataggg aaatcttcgg tgagggcgta
     3421 gcccattccc atgtgcaggg caccttcaat ttgaccctca aagagcatac ggttcataat
     3481 tttaccggca tcgtgggcgg caactagttt tcttaaacgt ccctcatcat caaggattgc
     3541 cacttgggct gcatacccat aagcaaaatg aacaacgggg ttttcggttt cggcacccgg
     3601 tttactcgtc cagtcgcaga cgaatttgcc cctgtaggtt ctccctgcca gctgttcgag
     3661 tgaagcttca gctaaatcgg ctttaagagc tgcggaagcg ttgattaagg cattggcaac
     3721 aagtgcggta gcccttgagg aggttgtcat acccgttgga acaccggctt cggtgtttac
     3781 tattacttca acaatcgaag ggtctatacc ggtttcctga tgaagtgtct gaattgccat
     3841 ggtgtgaact ccctgtccca tttccgacca gccatggtga atttcaacac gcttacccga
     3901 aatgatcttt atgagtacct cgctatcgtc aaccataccg ttgcctacgc cggtattttt
     3961 aagtcctgcg gcaatgcctg caaaccttgc cgagatgaag tcatccttaa ctgctaaaag
     4021 gcactttttc aatcctacac cacttaaaat ctgtccagta gcggttttag caccattctc
     4081 cagagcattg tcaaagcgaa tttgccatct gtcgaaacct gcctgtcggc aaatatcatc
     4141 aatacagctc tccagggcaa aggttacctg gggtactcca aaacctcgca tagctcctga
     4201 tgggatgttg ttggtgtaaa cagttttggc cgatatgtca acagccggaa tggtgtatcc
     4261 ccctgtggcg tgccccacca cgcgttccat tacctttgtt cccaccgagg cgtaagctcc
     4321 ggtatcgcca atagcatcaa gtttaagcgc cgtgaatttt ccctttgagt tgcaagccaa
     4381 gctcattttc atccacacag gatgcctctt aggatgcatt ctcagcgatt cctccctatt
     4441 cagatgaatc ttaatcggca ttccggagag ataggcacac agggcggcat gcccctgcac
     4501 ggttaggtcc tccttacctc caaaccctcc tccattctga acctgaacaa cttttacctt
     4561 ttcctcggga atatcaagaa atttggcaat ttgacttctg tctatgtaaa ctccttgccc
     4621 ctggctatac aactttacac catcaccatc gggtaatgcc aacgctgttt cagtttccag
     4681 aaatgcatgc tctatgcgct gtgtttcata gacatctgat gaaatgtagg ctgcttcctc
     4741 gaatgctttt ttcacctcac caaaacgtat tgtgcaggtt tccagcacgt ttgacctttc
     4801 agtatgaaca ttcggactgt taggaagtat ggcttgatga atatcggtta ccgggctaaa
     4861 aaccctgtag gttaccctga tttgctctac agctttgcgt gctgtctcct cagttgtggc
     4921 tacaacactg gctactacat cgccaatgta atgtgttgtt tcacctttac caaccatcag
     4981 cggccaatcc tgaaatacca acccgctgaa gtggttaccc ggtatatcct ttgcagtgta
     5041 tattcctacc accccatcaa tgctcatagc aggggcaaca tcaatgctga gtatctcggc
     5101 acgtggatgg tcggagaatt tcaatgcgcc aaaaagcatt ccgtctatcc gtaagtcgtc
     5161 aacaaagggc ctctcgccca gagctgttgc ttcggcctgg tactttggat agcgttctcc
     5221 aatttttgct tcggcaacga cagtaggttc taactcgcct gcaagttctt taaatgaggc
     5281 gtctatagca tcaataattt taacgtatcc ggtacacctg caaaggttag gggttattgc
     5341 tcttataatc tcgttcctgg agggttttgg gttttggttg taaagcccct ttgccctcat
     5401 aatcatacca ggtgagcaga aaccacactg aacggcaccc ctgagggcaa aatttcgacc
     5461 caacgttttc ctaaatgttt catcgagccc ctcgagggtg aagacaaccg atccctcaat
     5521 gtctcccatt tttgttcggc aggccatttt aatcttaccg tcaacttcaa ctgaacatgc
     5581 tccacataca ccctgtccgg aacatccatc cttggctgca gtaaggtgca ttgtatttcg
     5641 aagaaaaacc agcaggttgc tttttgggtc tcctgtaaac tcaacaattt tggagtttag
     5701 cgtaaaagtt atcatagatt tttagaagat gattgaactc gaatagctgt cttatccagg
     5761 aacaagctgc aatttactaa tttgaaggga ttgcgtttgc ctattagctg gttgattttg
     5821 gttaaaatgc actattttgg cttaaactat tcatttccag gtatcaacaa gttccttaag
     5881 agcatcaatt tttggtttaa tggcttttgg aaattgaaca atgggcgaaa cggcttttag
     5941 gtctttcaga ccgctgccgg ttaacagaac catgtttgag gtattccctt caactttatt
     6001 ttgcctgaag aacgatatta ggcctgcaag tgctgccgaa gcagcaggtt cggcaaagag
     6061 tccggtattt cttgccagaa tggctgaggc ttcaagaatt tcaccatcag taaccgttac
     6121 agcttcgcca ttgtatgttt taatgtattg acgggccatg tagaagttac ggggaatatc
     6181 taccgaaatg gaatcagcca gggtttggct ggtttgcgaa caaaaaacat cggtgaaaag
     6241 attaccaaca agattgctac tacctttagc ctgaacggca acgatggtag gcattctgtc
     6301 aatcaacttc aacatcagaa gatcctcaaa tcccttgtaa acacctgaga tgataacgcc
     6361 atcgccaacg ggaacaaaaa ttctatcggg cagctggccc ttcagctgct caaagatttc
     6421 gaacgaaacg gtctttttcc cctcaatggt tagcgggtta aaagcggtat tcctgttgta
     6481 ccagccaaat gtttcggatg atttcacact caagtcgaaa gcttgatcgt aggatccatc
     6541 aacaggaaca attgatgcgc catacattac tatttgcgtc aacttggctg caggagcatt
     6601 tttggggacc ataataatgg ctttttgacc ctgagaggcg caaataccgg ctagcgatga
     6661 gcctgcattg ccggttgatg cggttactat ctcatttttc ccattttcga gggcataggc
     6721 cgaaaccaat gccgaagctc tgtccttgaa cgaaaatgta gggttttgtg agtcatcctt
     6781 cagaagcagg gtaaaaggaa gatttttccc ctccatacgg ctgtacttgt aaagaggggt
     6841 tttcccaacc tttagctgag gcaagctgga tagtgagcgt attggtaaaa gatcgatgta
     6901 tccgtttcct gctagctgcg ctatgttttg gcaaatggag tcgtagtcat agattacctt
     6961 aagtacgcct ttaggcgggt tttccggttg atttagctta ctgcacgatg ggcaaaggta
     7021 aatatctgac gagccgccat actcctttcc acagcaacag catcggtaaa tatactttgc
     7081 actgttcatc ggattatcta taagcctttt gtcgaatatg cggctacctg agggacttaa
     7141 gtagcccggt tctgtcgtta aattcctcaa tcagctcatc ccaccggcta acctggttga
     7201 aaaggttttg ccagatattc ctgtcgtacc agagctggtt gaggtcttca acattcttgc
     7261 cctgttgctc aacccatgtg tagtacttca ggttgtgaat agccttgcgg tcggtatagg
     7321 ttagctcttt aatccagtcg gttgatacgc caagcaggca tttctcgtaa tccttaattg
     7381 cctgaatctg ggagtaagca ccacgctcgt tggacagctc ttcaagtcgg gaaaggtaca
     7441 tttcggcaga atcggttgct atggtaacaa ttatatcgtc ggatgtcatc tcaaagtagc
     7501 gtgccgtttt tattgccgag agcaggttgc ttatgcccga aatgccaagc aggttaagca
     7561 tatttacagt ttcaatggga atacccaggg aggcaaggta tgacttacct tcgggctcgt
     7621 tgaaaagcct gaaaagtctc atgcaatcct cgtcatcaat ggccgttacc acgtcggtat
     7681 tcttcacatt atgtatccag ggaacatgct tgtcaccaat gccttcgatt cgatgccctc
     7741 caaaaccgtt gcagtaaagt gttgggcatt gcagggcttc ggaggctacg acttttacat
     7801 gcggcgcaat ggttctcagg tagtcgcctg cggcaatggt accagccgaa cctgttgccg
     7861 aaacgaaggc agcaaggttt ttttgcccac ctttaaggtt gctgaaaacc tcctcaatgg
     7921 cttttcctgt aacgttgtag tgccatgcag aattaccaaa ttcatcaaac tggttgaata
     7981 ttatatagtt gggtttagta cggcgaattt cccagcactt atcgtaaatt tcctttacgt
     8041 tcgactcgca cccgggcgtt gctatcacct ccgagccaac ataatcgcga agccatgtaa
     8101 accgttcgct gctcatctcc tgtggaagga tggctactga atcggcaccc atcagcttgg
     8161 agtcgaatgc tccaccacgg cagtagttgc ctgtggaagg ccaaactgct ttttgcgtgg
     8221 tagggtcaaa acccccggtt gttatttggg gggccaagca gccataggca gcacctacct
     8281 tatgtgcacc cgtggggaac catttaccga taagcataac tattcgagcc ttcacgccgg
     8341 taagctccga aggcagctca ataaagttaa ccccgccgaa gagcccaccc ttctctttcg
     8401 gttcgttttt ccaagtaatc ctgaaaagat tcaaggggtt tatttcccat aacccaatac
     8461 ctcttagctg ctcttgaatc tttgatggca ctagctcggg gttttgctgc tgcttgatcg
     8521 ttgggagaat gatgcccttt tctctgaaac ggcctacagc attttccaac gctttggcat
     8581 ttgaaacgca atcaactatt tttatcataa tatgtaaaaa aaatattttc ccaaaattag
     8641 tattggtata ataaatgact attaatattg ataaaaataa gaaataaagt taataaataa
     8701 ttaaatattg agaaaattga tatttttgta tggagatttt ggagaagtca taggcttttt
     8761 tgattatcga tcatgttagc cttaagctta aacgaagcca actttaattt gcagccaagt
     8821 gtgcctagta agtaatgttt actgaagata aatcatataa atttattgat tcccatgaag
     8881 aatattgatg aaatagacgg caagttgcta aacatactcc aggacaacag cagggtaacc
     8941 atcagggagc tatccgagaa gcttcatctt tctaccactc ctattcatga gcgaattaaa
     9001 aagcttgaaa aatcaggtat tatcaagcag tatgtaacac tgctcgatcc caaaattctt
     9061 gggaaaaagc taacagtatt catttcggtg tcgctttcaa gccatgtaaa ggaggttata
     9121 gacgagtttg agagggaaat taaagtgatg cccgaggtaa tggagtgcta ctacgtttca
     9181 ggaacttggg atttttttat caaaattcaa tgtaccgata tggacgacta ccataacttt
     9241 gtcataaacc gattttcgag gttaaagaat atcacgcaat tttacagctc gtttgtaatg
     9301 tccgagtcga aagtatcgta taagcaccag ctgtagctag catgtgtggc tactgctaga
     9361 tgtaacgttt ttttgcagtt gcttttgagt tgcctcggag taaacattca tagtttgaag
     9421 ggtaattgtt tttatgttaa attcctgaat taccttttct cttatgagga tggactcatc
     9481 cttatactga actcgatttt gaaagaattt attgatttct gacagaagcg attcccgggt
     9541 gggtggaata ccgtttatat caacaaaatt attcaaacgg tatgccgaat agttccgtag
     9601 gttaactata ctgcccattc ttttacagtt cactgaaatt acgttgaggc cgttagccag
     9661 cgattctaca gctactcttc caacccctag caccagctcg ggtaaaattt cctgctggtt
     9721 gaaattctca acgtacccgc atattcttac aacctctcta tgtaggctct ggtttagttg
     9781 ggatgctact ttttttaaat attcaacctg ctttccttca ccgaatatcc ataagcttgc
     9841 ctgagggaag ctggctgtaa catcgggcat taatccaata agcatgtcaa caacctcttt
     9901 gtgatggcta tccatccggc tgatgtatgc cattccataa ggaatacgct ttggtataac
     9961 tttttgaaag tttatcccat ttggaatcac aaccgatttg tggttgattt cagagtaata
    10021 ttttgatacc ttcagtactt gatggcttac aaaaataatt tttgaggttc tcgtgcgtga
    10081 aaacgatgag cgaaggtcga atctaactct gccatgaaca gtggctacta cgggtgttcc
    10141 tgttatgaat cctgcccaac aggcagctaa aatggataga cgctgatggg catgaattac
    10201 ctcaatctga tgttttttta taaggtaaat caaaaaaaag gtttggaata ccagcagctt
    10261 acttagctgg cttgcttgaa cgaagtgaac tttttggttt aaaagcttga tctgctttcc
    10321 tgatggtcca accaggtaaa cgtcgtgagc ctgagctgcc agctcattgc ttagctctcg
    10381 aacgtgattt tcggcacctc caaaatcaaa gtgggttaca acttgaagta ttttcaatgt
    10441 gtggcaaatg tgtcggcaaa tatagttttt ggggatgaaa aaaagttcag aagtaatgaa
    10501 aacgtaaacc gtatatttgt aaggccgtgg caaaatgtaa aaaactcccc acgggtagta
    10561 ccttcaatag ttagtccaac gctactcctt catttaaccc tagtaattat tctcaacagg
    10621 gcttcaaaat gaaaagggca gtttccttaa aaagcattta cacgaatgca ttatataccc
    10681 gggatatgca gaaaattttc aagggtggct tgttaacaaa ttgatgttaa gtatgttgaa
    10741 tcttgaatcc gtcaggagcc ggacaagttc cgccagtagg cggacaggtt cttgaatc
//
LOCUS       DAOSHM010000074        10721 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_5190_length_10721_cov_21.041815, whole genome shotgun
            sequence.
ACCESSION   DAOSHM010000074 DAOSHM010000000
VERSION     DAOSHM010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 10721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10721
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5190_length_10721_cov_21.041815"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..684
                     /locus_tag="PK839_09330"
     CDS             <1..684
                     /locus_tag="PK839_09330"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HOG72977.1"
                     /translation="RIQTSILNKMEKRILVAMANRMPSWITSDNLTVVGVIGALTTGV
                     GYILSSWNLNFLWLASFGLILNWFGDSLDGTLARVRNAQRPIYGFYIDHNIDGLTITI
                     MCIGAGLSPMIKFSTAMLILVGYLLLSIFTYINAYLKGEFRISYGNLGPTEFRLIVII
                     INTMFIYFPFKDMYLHIMKYEVGVYDAIGFGIAILLFFIFLISFFVFLRKYKQVDPPR
                     WVDNHNNSN"
     gene            688..1152
                     /locus_tag="PK839_09335"
     CDS             688..1152
                     /locus_tag="PK839_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HOG72978.1"
                     /translation="MMPKYFKFIASRLIGTIVDTLVLWLLTSFIFSSYVGAYIIAPTI
                     SFEFAVFSNFLFSYFWIWSKRVAVKNNRSFFSHFLSFNISSFLGFIVKMGFLLLFSKL
                     LKWSVVYCNFLALLISGVVNFGLSELVVFKKSPVVDSVDEKILVRRNKSGKF"
     gene            1164..2372
                     /locus_tag="PK839_09340"
     CDS             1164..2372
                     /locus_tag="PK839_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HOG72979.1"
                     /translation="MGKLQERYSQYTLPQQIKAEGIYPYFRAIESEQSTVVTMKGKQV
                     LMFGSNSYLGLANHPRIKEAAKKAIDKYGTSCSGSRFLNGTLDIHLELEEKLAKLVGK
                     ESAICYSTGFQVNLGVVSALCSRGDYLLLDCLDHASIIEGSRLSFSKILKFDHNDMSS
                     LEAKLKLCEPDRIKLIVVDGIFSMEGDIANLPVIVQLAEKYDASIMVDDAHSLGVIGD
                     GGRGTASHFNLTPKVDLIMGTFSKSFASLGGFIAGDESVINYLRHNSRSLIFSASMTP
                     ASVASVSTAIDIMLEEPERMKHLWDLTRYAHEAFKSHGIYIGKSETPIIPLYVRDDIK
                     TLQLTKMLLDEGIFINPVITPAVPKEDSMIRFSLMATHTFEQLDIAIDKIVGSFKKLG
                     LLTTSTVKNP"
     gene            2384..3181
                     /locus_tag="PK839_09345"
     CDS             2384..3181
                     /locus_tag="PK839_09345"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOG72980.1"
                     /translation="MKVVLITGVSSGFGRAIVLRLANKEFKVYGTVRRKVDPIPGVSY
                     LNMDVTSRESVEKAVGEVYGKEGRIDVLISNAGMGIAGPLEFTSIDDVQHQMDVNFLG
                     MVRVVQAVLPIMRNQKGGRIICISSIAGLIGLPFQGVYSASKFAIEGYCLALRTEVRP
                     HNVEVVLVNPGDFSTSFTRNRSVVDSSEAKEAYPNYIKTMKVVEQEENRGLSPEFLAK
                     KIEKIINRKKCPTRCVVATPVQRASVFIKRILPEGLFYRIIQKYYMS"
     gene            3220..4347
                     /locus_tag="PK839_09350"
     CDS             3220..4347
                     /locus_tag="PK839_09350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HOG72981.1"
                     /translation="MKVTIKEVTDKRLLKKFIKFPNKLYKGNRYYVPQFIASEFSTLS
                     KEKNPAFEFCEARYWLAYNEDNAIVGRIAGIINNRYNQKTATKYVRFGWLDFVEDADV
                     LRALFDTVENWAKANGAQYLHGPLGFSEFDASGILIEGFNELPTAYGKYNYPYYQSMV
                     EQLGFEKEVDWVEYNIKVPDSVPEKYSKMADIIEERNQLHRLKFSSKRELLAYTDEIF
                     QLLNREYSSIHGFAELTQGQIDDLKKQFVPLIQLKFISVVKNAQGEVVGFGICLPSLS
                     KAIQKSKGRMFPFGFIYLIKALKYNDTLDTLLIAIHGDYKNKGVNALIFNDIGKAIIE
                     SGITNIETTRELEDNFMVQNLWNKLEYRQHKRARCYIKRLS"
     gene            4384..4968
                     /locus_tag="PK839_09355"
     CDS             4384..4968
                     /locus_tag="PK839_09355"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOG72982.1"
                     /translation="MTDEVRDMIVESAATFFNRFGFKHTTMENIAESIHKAKGLIYYY
                     FKSKEELFKEVLWNDIRRIKAHLMATANSEDDTHITLKKFVHARLELLEGATSYHEVI
                     KAQIYHKSHVFLDDIKNDFEDFEKSQLSSIIRRGEQRGDFKVESIPSTVNGILTVLGS
                     LDFSLFLIKKRVELDTINDEISTLIVNSVRREIA"
     gene            complement(5092..7317)
                     /locus_tag="PK839_09360"
     CDS             complement(5092..7317)
                     /locus_tag="PK839_09360"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOG72983.1"
                     /translation="MTRRKYIINWLIVVFDIWLITNSFPIYATSTANPDDSRLQQFSF
                     NDTIARIDLRISEFEKNGNRAELAKALTEKAGIYFRVARYDDALMTANQALLVANEEG
                     REQLLEPIYHLLSLIHIENELVFEAAEYLYAGYQKSLDMKDTANIGWYLVNLSEVEEY
                     LGRVSNAMEINLTAIDFFKNTSDSLSLAKIYRAQGVAHAELKNFLTAEDYFKKAFRIF
                     NSCGDSLNMGLVLMDQASLNLKELNLYEAQSLIGQAMKLLEKQSQKFYLRSRCIYGEL
                     LVQRRSFTEAIEMLKETLMIQLSINDLYGYTATLSALGRAYLGSGNRQMAIDSYTRCA
                     KMADKANLINFKRDSYRNLASILGSEGNSSKAYYYLSSYVTLTDSLFNLQKISEANRL
                     ENQAMLRMKEKEIAQQKEQIILNSSQLRQESVKQKFLYIIIILSFGVTAAVVYSYRQK
                     KTANALLTEQNNRIEKQRDMLEFRSREIMDSLNYAQRIQRAILHSSLQPEDFFPDSFL
                     IFIPKEVVSGDFYWFKAVDNLKLFAIADCTGHGAPGAFMSIIGMFGLNQIITEYGQTH
                     PGDVLNQLNELFYHSFEQREGAEIFDGMDIGFCEYNTTTKELKFAGANIYLYILRKSS
                     LPAPSSITLHKTTDYTLYQVKCNRQSIGYMAEKIEFSTHSITLMADDTIYLFTDGFVD
                     QFGGPQGKKFRYNELRALLCEMATIPMSEQKSRLLEIFTEWKGGYVQVDDVTMLGVRV
                     T"
     gene            7531..8673
                     /locus_tag="PK839_09365"
     CDS             7531..8673
                     /locus_tag="PK839_09365"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOG72984.1"
                     /translation="MKAIPIYLSLLLALFSCGNRAESRGGSILEMNANYPPKYAEGFT
                     IFNDSTGTKIRVFNPWQGASNVSLEYKLVPRGDISKFSSNPGAIPVPLERVVCMSSSH
                     LAFIEALGMGQRIVGVSGAGFAFSSALRAMVDEGKIFEVGYDQGINYEQIARLNPDVI
                     LCYGIGSESMAYLKKFSEMGMRIMFIGDYLESDPLGKAEWIKVLGCLFNCYGKADSIF
                     SSIESSYLKTKKQVERCSSKPTIFMNMPFRGVWYFPGNDSYMVKLVHDAGGSYVFENL
                     EGSRSFPLGIEKAFEAGMGSDIWLNPGSASSLTDITSFDTRLAQLPPVVSGKVYNNNL
                     RMSRGGGNDFWESGTVHPELILHDLISIFHPEIANDSLLVYYQKLE"
     gene            8726..9793
                     /locus_tag="PK839_09370"
     CDS             8726..9793
                     /locus_tag="PK839_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014775995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOG72985.1"
                     /translation="MERSSKNYGKSKERKPTGKVGMMVVLLAVLVFLLVFIDLLLGSV
                     RIPMSDVLKFIFTGSAGSEVSQNILFNFRLPRVLAAVLSGSALAVSGLLMQTIFRNPL
                     ADPSVLGISAGASLGVALVVMGSSALGLSFLGNQFSMVLAALAGSMLVLMLVMAVSVR
                     VRNVIVILILGMMFGFAASAVTSILQYFSSEVSLKSFVIWTMGSLSSISGNQLWIFAL
                     LVFLGLVLAMLVARPLNVLGVGENYAQLLGYNVKAIRMVVFISTSLLAGSTTAFVGPV
                     VFVGIAVPHVVRMAVKTSNHHVLLPTTLLAGAAFMLLIDTIAQLPGAGIVLPVNTVAA
                     LMGIPVVIYVIFKGSHFHAAS"
     gene            9780..>10721
                     /locus_tag="PK839_09375"
     CDS             9780..>10721
                     /locus_tag="PK839_09375"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG72986.1"
                     /translation="MQPAEVIGIKNLVVGYGEGAMSDFTYPPINANAYKGEMVALVGR
                     NGAGKSTLLRTISRLQPMMNGEITVNGATLESIGRSEFSQLVSFIPAEPVRLPNTTVS
                     DFITLARYPYHGWFDGLSHTDTTMVGKALKAVNLFDYRNRFLDRLSDGERQRVMVAFA
                     IAQDTPVILMDEPTAFLDLPSKFEIVRLLREQTLLGKTVIISTHDLQTAFGFVDTIWL
                     MLREGIRIGSPEDLVLTGAIDSLMDGTLVKFDMSSGQFKYTNSKTLSAYVSGSKRSLV
                     KWTAHALERLGYHVSSIVLDTTSLQVEVMETEGKIQWC"
BASE COUNT         2799 a         2266 c         2583 g         3073 t
ORIGIN      
        1 agaatacaga cttccattct gaataagatg gaaaaaagga ttttggtggc gatggccaat
       61 cgaatgccca gctggataac ctctgataac ctcaccgtag tgggtgtgat cggcgcatta
      121 acgaccgggg taggatacat actttccagc tggaacctta attttttatg gcttgcctcg
      181 tttgggctta ttctaaattg gtttggcgat agccttgatg gaacattggc aagggtgaga
      241 aatgctcaaa ggccaatata cggattttat atagatcata acattgatgg gctcaccatt
      301 accattatgt gtattggcgc cggcctttcg cccatgataa agttttctac cgccatgcta
      361 atactggttg gttacctgct tctgtcaatt ttcacctata ttaatgccta cctaaaaggt
      421 gagtttagaa tatcatacgg caacctggga cccaccgaat tcaggcttat cgtaatcatt
      481 atcaacacaa tgtttatata ctttccattt aaagatatgt accttcacat tatgaagtat
      541 gaggttggag tttacgatgc cattgggttt ggaattgcca ttctgctttt ctttattttt
      601 cttatcagct tctttgtctt tttaaggaag tacaaacagg ttgaccctcc ccgttgggtc
      661 gataaccata acaattcgaa ttagcccatg atgcccaagt acttcaaatt tattgccagc
      721 cggctaattg ggacaattgt cgatacgcta gtgctgtggc tgctcacgag ctttatcttt
      781 tcctcctatg ttggagcata catcatagca cctacaatct cattcgaatt tgcggttttt
      841 tcaaattttt tattttcata cttttggata tggagtaagc gcgtagctgt aaaaaacaac
      901 agatcttttt ttagtcactt tttatcattc aacatatctt cttttctggg atttattgtt
      961 aaaatgggat ttttgctact atttagcaag ctgttgaagt ggagtgtggt ttactgtaat
     1021 tttttagcac tgctcatttc cggagttgtc aacttcggac tttcggagct ggttgtattt
     1081 aaaaagtccc ccgtagtgga ctcggtggac gaaaagatat tggttcgtag aaataaatct
     1141 ggtaagtttt aacttgaatg gatatgggaa aacttcaaga acgttactcg cagtacaccc
     1201 tgccccagca aataaaggct gagggaattt acccttactt tagggcgatt gaatccgagc
     1261 aaagcaccgt ggttaccatg aagggcaagc aggttttaat gtttggctct aacagctatc
     1321 tgggtttggc caaccatccc cggattaagg aggcggcaaa gaaggcgata gacaagtacg
     1381 gcaccagctg ttcgggttca agattcctga atggaacgct cgacattcac cttgagctgg
     1441 aggaaaagct ggctaagctg gttggtaagg aatcggcaat ctgctacagc acaggcttcc
     1501 aggttaatct gggagttgtt tcggctcttt gtagccgtgg tgactacctc ctgctcgatt
     1561 gcctcgacca tgcctctatt atagaaggaa gccggctgtc attttccaaa attctgaagt
     1621 tcgaccataa tgatatgagc tcgctggaag ccaagcttaa gctttgtgag cccgaccgga
     1681 ttaagcttat tgtggttgac ggaatattct ccatggaggg cgatatagcc aacttgcccg
     1741 tcattgttca gctagccgag aagtacgatg caagcataat ggttgacgat gcccattcgc
     1801 tgggtgtaat tggggatggg ggcaggggaa ccgcctcgca cttcaacctt acccctaagg
     1861 tggacttgat tatgggaact ttttcaaaat cctttgcctc actgggaggt tttatagccg
     1921 gagacgagtc ggttatcaac taccttaggc ataactcccg ctcgctgatt tttagcgcca
     1981 gcatgactcc tgcctcggtg gccagcgtta gcactgccat tgatataatg ctcgaagaac
     2041 cggaacgaat gaagcatctt tgggatttaa cccgttatgc ccatgaggct tttaagagtc
     2101 atggcattta cattggcaaa agcgaaaccc ccattattcc tctttacgtt cgcgatgata
     2161 ttaaaaccct tcagcttact aaaatgcttt tagatgaggg aatattcatc aaccctgtta
     2221 ttacacctgc tgttccgaag gaagactcca tgatacgctt ctcactcatg gctacccata
     2281 ccttcgaaca gctggacatt gccatagata aaattgtggg ttcatttaag aagctcgggt
     2341 tgcttaccac ctcaaccgtc aaaaatccct agcctacaag caaatgaagg ttgttctcat
     2401 aacgggtgtt tcctctggat ttggtcgcgc tattgtactg cggcttgcca ataaggaatt
     2461 taaggtttat ggcaccgtgc gccggaaggt cgatccaatc ccaggtgtta gctacctcaa
     2521 catggacgta acgagccggg aatcggttga aaaggctgtt ggggaggttt atggaaagga
     2581 ggggcgtata gacgtgctga ttagcaatgc gggaatgggt atagccggcc cacttgagtt
     2641 tacaagtatt gatgatgtgc aacatcaaat ggatgtgaac tttttgggaa tggtaagggt
     2701 agttcaggct gttctaccaa ttatgcgtaa tcaaaaaggt ggtagaataa tctgcattag
     2761 ctctattgcg ggtttaattg ggctaccttt tcagggcgtt tactcggcca gcaaatttgc
     2821 catagagggg tattgtcttg ccctgcgaac ggaggtaagg cctcacaacg ttgaggttgt
     2881 acttgtaaac ccgggcgatt tctccaccag ctttacccgc aacaggagcg ttgttgattc
     2941 atcagaggca aaggaagcct accccaatta tatcaaaaca atgaaggtag ttgagcagga
     3001 ggagaacaga gggctttcgc cggagtttct tgccaaaaaa attgagaaaa ttataaatag
     3061 gaagaaatgt cctacccgtt gcgttgtggc tacaccggtt cagagggcat ccgtatttat
     3121 caaaagaata ctacccgagg gtttgtttta ccgaattatt caaaagtact atatgtcctg
     3181 aaggtgcggc tattacttac ctactaattc ctgaattgaa tgaaagttac tattaaggag
     3241 gttacagaca agaggttgtt aaagaaattc attaagtttc ctaataagct ctataaaggc
     3301 aacaggtact acgtacccca attcattgcc agcgagttta gcacattatc aaaagaaaaa
     3361 aatccagctt ttgaattttg tgaagccagg tactggctgg cctataacga ggacaacgcc
     3421 attgtaggtc gtattgccgg aatcataaac aataggtata accaaaaaac ggcaacaaaa
     3481 tacgtacgat tcgggtggct cgattttgtt gaggatgccg atgtgcttag ggcgttattc
     3541 gatacggttg agaattgggc caaggcgaat ggagctcaat accttcatgg ccctctagga
     3601 ttctcagaat ttgatgcttc tggaattcta attgaggggt tcaacgagct gccaactgcc
     3661 tatggtaagt ataactaccc ttactaccag tccatggttg agcagctggg atttgagaag
     3721 gaggttgact gggtggagta caacatcaag gtgcccgact ccgttccaga aaagtactcc
     3781 aaaatggccg atatcattga agagcgtaac cagctacatc gcctcaaatt ttcgagtaag
     3841 agagagctgc ttgcctatac cgatgagata ttccaacttc ttaacaggga gtactccagc
     3901 atccacggct ttgccgagct aactcaaggt cagatagatg accttaagaa gcagtttgtc
     3961 ccgctgatcc agctaaaatt catttcggtg gtcaaaaacg cacaggggga ggtggtaggt
     4021 tttgggattt gtctcccatc cctatcaaaa gccattcaga aatcaaaagg acgaatgttc
     4081 cctttcgggt ttatctacct cataaaagca ctgaagtata acgacacact tgataccctg
     4141 ttaatagcca tacatggcga ttacaagaat aagggcgtta atgcgcttat attcaacgac
     4201 attggaaagg ctattattga aagcggcata acaaacattg aaaccacccg cgagctggag
     4261 gataacttca tggttcaaaa tttatggaac aagcttgagt accgtcagca caagagggca
     4321 agatgctaca taaaaaggct ttcataacag ctttttgtat aaccaataag gatttcaata
     4381 gctatgacag acgaggtaag ggatatgatt gtggaatcgg ctgccacctt ttttaaccga
     4441 tttggtttta aacataccac catggaaaac attgccgaaa gcattcataa ggctaaaggc
     4501 ttgatatact actatttcaa aagtaaggag gagctgttta aggaggtttt atggaatgat
     4561 atcaggcgca ttaaagcgca tctcatggca acagccaact ccgaggatga cactcacatt
     4621 acactaaaaa agtttgtaca tgcacgttta gagttgcttg agggggctac cagctatcat
     4681 gaggttatta aggcacaaat ttaccataaa agccacgttt ttttagatga tataaaaaat
     4741 gactttgagg attttgagaa aagtcagcta tcaagtatta ttcgtagggg agagcagcgc
     4801 ggcgacttta aagttgaaag catcccttca acagtcaatg gtattttgac agtcttaggc
     4861 agcctcgact tttcattgtt tcttataaaa aagcgtgtgg agttagatac cataaatgac
     4921 gaaatttcca ccttgattgt caacagcgtt agaagagaaa ttgcctaatg taaaaacaag
     4981 aaaaaagttg tgaacagctt tgagctgtgg gatgtgagac ttgatccgtg agaaaattta
     5041 atgttaaagc tataacttct ctccatccgc gagaatctcc gtactccttt tttaggttac
     5101 tctaacgccc agcatggtaa catcatccac ctgcacgtag ccacccttcc attcggtgaa
     5161 aatctccagt aacctgctct tttgttcgct catgggtatg gtagccattt cgcagagaag
     5221 tgcccggagc tcattgtacc tgaatttttt accctgaggg ccaccaaact ggtcaacaaa
     5281 gccatcggtg aacaggtaga tggtgtcatc ggccattaac gtaatgctat gggtggaaaa
     5341 ttctatcttt tcagccatgt aaccaatcga ctggcggttg cacttaacct ggtaaagggt
     5401 ataatcggta gttttatgaa gggtgattga cgaaggggcg ggcaagcttg atttgcggag
     5461 gatgtagagg taaatatttg ctccggcaaa ttttagctcc ttggtggttg tgttgtactc
     5521 acaaaaacca atgtccatgc cgtcaaagat ttcagcaccc tcacgctgtt caaacgagtg
     5581 gtaaaagagc tcgtttagct ggttgagcac gtctcccgga tgtgtttgcc cgtactcggt
     5641 aataatctgg ttgagcccaa acatcccaat aatactcata aatgcccctg gcgctccatg
     5701 tccagtacag tctgcaatgg caaacagctt caagttatca accgctttaa accagtaaaa
     5761 atcgccacta accacctcct ttggaataaa aatcaggaac gaatctggaa aaaaatcttc
     5821 gggttgaagc gatgaatgga gtatagcccg ctgaatacgc tgggcatagt tgaggctatc
     5881 cataatttcg cgtgacctga attccagcat atccctttgc ttttcaatcc tgttgttttg
     5941 ctcggttaac agggcatttg cagtcttttt ttgtcgatag ctgtaaacaa cggcagctgt
     6001 tactccaaac gaaaggataa tgatgatgta aagaaatttt tgcttcacgc tttcctgcct
     6061 tagctgcgag ctattcaaaa tgatttgctc cttttgctgg gcaatctcct tctccttcat
     6121 tctcagcatc gcttggttct ccaaccggtt tgcttcgcta atcttttgta ggttgaaaag
     6181 gctatcggtt agggttacgt agctcgatag gtagtagtat gccttactgc tgttcccctc
     6241 ggagccaagt atggacgcaa ggttgcggta tgagtctctt ttaaagttaa tgaggttggc
     6301 cttgtcggcc atcttagcgc agcgggtata gctgtcaata gccatttggc gattacccga
     6361 acccaggtat gctcttccga gtgccgatag ggtggcggtg tacccgtaaa ggtcgtttat
     6421 tgagagctga atcatgagag tctccttcag catctcaatg gcttcggtga agctccgtcg
     6481 ttgaacaagc agctcgccgt agatgcacct gctgcggagg tagaattttt gactttgctt
     6541 ttcaagcagc ttcattgcct gcccaataag gctttgagcc tcgtaaaggt ttagctcttt
     6601 gaggttgagg cttgcctgat ccatcagaac caaccccatg ttaagcgaat cgccacagct
     6661 gttgaatatt cgaaaggcct ttttaaagta atcctcggcg gttaggaagt ttttcagctc
     6721 ggcatgtgct actccttgag cacggtatat tttggccagg cttagcgagt cactggtgtt
     6781 tttaaagaaa tcaattgcag tcaggttaat ctccatagcg ttagaaaccc ggcccaggta
     6841 ctcctcaact tcacttaggt taaccaagta ccagccaata tttgcggtat ccttcatgtc
     6901 caggcttttc tggtagccag cataaagata ttcggctgct tcaaacacca gctcattttc
     6961 aatgtggata agggataaca gatggtatat tggttcaagg agttgttcac gtccttcctc
     7021 gttggccaca agcaaggcct ggttggccgt cattagtgca tcgtcatagc gggctactct
     7081 aaaatagatg cccgcctttt ccgttagtgc cttggcaagt tcggccctgt taccgttttt
     7141 ttcgaattcg cttatccgca aatcaatcct ggcgatagta tcattaaaag aaaactgttg
     7201 caatcggcta tcgtcagggt tggcggttga cgtagcataa atgggaaatg agttggtaat
     7261 caaccatata tcgaaaacaa ctataagcca atttattatg tatttccgcc tagtcatcct
     7321 aaatttgaaa tcaaattttc aatgtgtgaa ggtagcttct tatcaggaat attcttactc
     7381 ttacccagaa ttcttttttg tgccatttca acatgatggc agcgctttag cttatcattc
     7441 agcttttttg agctgctgcc tactttctgg atgtaatttt tcacaaccgg ttggtggata
     7501 tgggcatatt cagctacttt agcggaaaaa atgaaggcca tacccatata cctatccctg
     7561 ttgctcgccc tgttttcatg tggcaatcgt gcggagagca gaggtggctc aatcttggag
     7621 atgaacgcca attatccgcc aaagtatgcc gagggtttta ctattttcaa cgatagcacc
     7681 ggcactaaaa tacgggtttt caacccctgg caaggtgcca gcaatgttag cctggagtat
     7741 aagctggttc caagggggga tattagtaaa tttagctcca accctggtgc aattccggtt
     7801 ccgctggaac gtgttgtttg catgtcgtcc agccatttgg catttattga ggcgttggga
     7861 atgggacagc gaattgtggg agtttccggt gcggggtttg ctttcagctc cgcgctgagg
     7921 gcaatggttg atgagggaaa aatattcgag gttggctatg atcaggggat aaactacgag
     7981 cagattgccc ggttgaatcc agatgtaatt ctttgctacg gaattggttc cgaatcaatg
     8041 gcatacctta aaaagttcag cgaaatgggg atgcgcatta tgtttatagg cgactacctg
     8101 gagtccgatc cgcttggtaa ggctgaatgg attaaggttt taggctgtct ttttaattgc
     8161 tacggcaaag ccgattcgat ttttagtagc attgaaagta gctacctgaa aaccaaaaag
     8221 caggttgagc gttgcagctc aaaaccaacc atttttatga acatgccctt tcggggtgtg
     8281 tggtatttcc cgggtaatga cagctacatg gtgaagctgg tgcatgatgc cggaggcagc
     8341 tatgtttttg agaacctgga gggtagccga agcttcccac tgggaataga aaaagcattt
     8401 gaggccggaa tgggcagtga tatctggcta aacccgggtt cagcaagcag cttgacggat
     8461 atcacctcgt ttgatacgcg cttagcccag ctgccaccag tagtttctgg caaggtgtat
     8521 aacaataacc tccggatgag ccggggaggg ggtaacgact tttgggaatc gggaacggtt
     8581 catcccgagt tgatcctgca cgaccttatt tccattttcc accccgaaat tgcgaacgat
     8641 tcacttttgg tttactacca gaagttggag tagcttaaca actaacgaga ttaccattga
     8701 ttttggcgta agctgagtaa tagtgatgga gcggagcagt aaaaactacg gtaaaagtaa
     8761 ggaaagaaag cctacaggga aggtaggcat gatggtagtt ttgcttgctg tgctggtttt
     8821 tttactggtt tttattgatt tactgttggg gtcggttaga attcccatga gtgacgtgct
     8881 aaagtttata tttaccggaa gcgcaggcag cgaggtaagc caaaacatac tgtttaactt
     8941 caggttgccc agagttctgg cagctgtgct gtcgggttcg gctctggcgg taagcggctt
     9001 gctgatgcaa accatatttc gaaatccctt ggccgaccct tcggtgctgg gaattagtgc
     9061 tggtgccagc ttgggggttg ctttggtggt gatgggttca tcggcgttag ggctttcatt
     9121 tttgggaaat caatttagta tggtgctggc cgcattggca ggttcaatgc ttgtgctcat
     9181 gctggtaatg gccgtttcgg tgagggtgcg caacgttatt gttattctga ttttgggaat
     9241 gatgtttggc tttgctgcct cagcagtaac cagcatttta cagtatttta gcagcgaggt
     9301 atccctcaaa tcgtttgtta tttggactat gggcagccta agtagcatta gcggaaacca
     9361 gctatggatt ttcgccctgc tggttttcct tgggcttgtg ctggccatgc tggttgcccg
     9421 accattgaac gtgctgggcg ttggagaaaa ttacgcccag ctgctgggct ataacgttaa
     9481 agctatcaga atggtagttt ttatctcaac cagcctgctg gccggttcaa ccactgcatt
     9541 tgtcggtccg gttgtttttg ttggtattgc tgttcctcat gtggtgcgaa tggccgttaa
     9601 aacttccaac catcatgtgc ttttacccac cacgctatta gcaggtgctg catttatgct
     9661 cctgattgat actattgccc agctgcccgg cgccggaatt gtgcttccag tgaataccgt
     9721 ggcagcgtta atgggcattc ctgttgtaat ttacgtaatc tttaaaggta gccacttcca
     9781 tgcagccagc tgaggtaata ggaattaaaa acctggtagt tgggtatggt gagggggcta
     9841 tgtccgattt tacctatcca cctatcaatg ccaacgctta taagggagaa atggtggcgc
     9901 tggtgggacg gaacggagcg ggaaaaagca ccctgctgcg caccatttca cggcttcaac
     9961 ccatgatgaa tggcgaaatt accgtaaatg gcgctacttt ggaatcaatt ggacgctctg
    10021 aattttcgca gcttgttagc tttattccgg cagaacctgt tcggttacct aataccacgg
    10081 tctctgattt tattacactt gcccgttacc cctaccatgg ctggtttgat gggctcagcc
    10141 acaccgacac gactatggtg ggcaaagccc ttaaggccgt aaacctgttt gactaccgga
    10201 atcgcttcct cgataggctg agtgatggcg aaaggcagcg ggtgatggtt gcctttgcca
    10261 ttgcccagga tacacctgta atcctgatgg acgagccaac ggcattcctc gatttgccga
    10321 gcaagtttga aattgtaagg ttactccgcg agcaaaccct gctggggaaa acggtcatca
    10381 tctcaaccca cgatcttcaa actgcctttg gctttgttga caccatctgg cttatgcttc
    10441 gcgagggcat tagaattggt tcgcctgagg acttagtgct aaccggtgct atagacagcc
    10501 tgatggatgg aaccctggtt aagttcgaca tgagctccgg acagtttaag tacaccaact
    10561 ccaaaaccct tagcgcttac gtctcgggaa gtaaacgaag cttggtaaaa tggactgccc
    10621 atgcgttgga acgacttggc taccatgttt caagtatcgt tttagataca acttccctgc
    10681 aggttgaggt gatggaaacc gaagggaaaa ttcagtggtg t
//
LOCUS       DAOSHM010000075        10718 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_5193_length_10718_cov_6.403451, whole genome shotgun sequence.
ACCESSION   DAOSHM010000075 DAOSHM010000000
VERSION     DAOSHM010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 10718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10718
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5193_length_10718_cov_6.403451"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(819..4352)
                     /locus_tag="PK839_09380"
     CDS             complement(819..4352)
                     /locus_tag="PK839_09380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72987.1"
                     /translation="MASIGNTTLQVGSDDTPASSVLTLKSTMELGNLTIAGVNSPTLR
                     LKDRSLTLKGDLTIGSGCTFDGAGSFNLTSKRHIVNNGMANLNVDTLFMAGSSSPPSS
                     ANQQITGNVRARNLIVEPETSVSLQPSSELEVDANLHIISGQLVDGANRIVVKGNVTN
                     NSSHVSSNPVTGGIVFNGTSLQRIYGNGEFGRIEVDNPNGVQLENSIAMENNLALTSG
                     ILQLQSNKLTLGLNAAVEGSGFDSTKMIAVGGGDYIQGIEKAFPTVPSAVPSDPYDPD
                     DPAYTFSFTFPIGVDNGTDKKYTPVELHVASNATQGTVNLFPVNKKHITFDETQTDVL
                     QYYWIMNSSGLSNFTSLMRMHYINGDVVGDESAYIGAKLYDNFWAKFQESEVPPIEVV
                     YEDDDFITFITDGVNLITGEYTAGIESHIPNEVPLYYSKQNGEWTSSSTWEREDGGLV
                     PVGGPVGQRVHIRTSHTVSVSSNYRRAYRTTINGRLELDSTKNHILGYVSGTGTLSTE
                     ISSLPSGSYDTFFACGTGGTMEWAGGTYGLPSSITSYNNITITGTGTKTFPNYDITIC
                     GDLRIDSSATLKQPGVPSDYRYTTIKGNTYLLDSATWDMGNRTWVNLEGNFTRDDSTS
                     FLTSYTNQTFKVNGTTDQAFIGVFNSDNQFNTLHFDNTGNISFNDSSGIRNYLYMNNG
                     HIQNPAGKPFMVAKSDGDGFYAYSNGLFEGSVSINLNSNSPKQFLPVGKNGIKKFIYP
                     LDLPLAVGYWTGEYFNSSPSAHIPPMRHDSLVSPLQSVSQSEYWRISGPNGVSSRIKL
                     TLDGTSDIASGISDINNLRIVYWNGTKWEIAGTGATITGNASSGTISTTASFTFNGAE
                     QFFTLGAIESVVVPTAQITSSDAGLCEGSTYQLVIALTGNSPWFIDYNDGTTSYHVDS
                     ILSSPHTINITGMITGSYTYVIDTVRDKDSVLGYIYGPNPTVNVYANPTQYIVTSGGN
                     ICGPTTTTIELNGSDVGFTYQLYRDAVYFGTELAGTGSALTFTGVDQSGTYTVRAFND
                     LNSSCYEWMNGSPFVDYAETPQAEVTGLISPSQMCEGEPVEIEITLTGVPPFTFSVAD
                     NHGGSWPDITVDVEELSGSGPYTYNFTTPDQLPTWSGPALPKVYHYNVTAITDDAGCG
                     PGTVVGPGVDVDVYKIPETGPQYHIPNNYAY"
     gene            complement(4403..10156)
                     /locus_tag="PK839_09385"
     CDS             complement(4403..10156)
                     /locus_tag="PK839_09385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72988.1"
                     /translation="MNAPLSNNPQGINIRIVGLLGLLFFLSVEGMAQYAPAGIGTSSE
                     NVLWLRSDQGTSTTTNGEPVSGWNDISGNANHAAQGTVGNQPIYISSGINGLPTIRFD
                     GTNDHLTVADADNLDNTNGVSVFVIAQPVSPDALARGLVSKRVSAGNEEAFYLFTYTG
                     RNLYFNASTYRINGNDPVTDQPQIFSAIYDGSVANPRSKIFYNGEESGNGNGPVSIAN
                     MASDLHIGTLNPGYAQAFNGDISEIIVYRDNLNKAQRLIVEAYLANRYNIAITPTSYS
                     STTHTQDFIGIGHSGGDRYSQTRNVGSGLLLSERNGTLDETNEFVFAGHDGTPHGFNT
                     NDLPTIPGVNLDQRWDRIYYVERVQNGVIDAGSTDVRITFDFTEAGLSLSTDRVYYLL
                     YRSGTSGTFSSVPGGTGVASNGKVSINLSSSNFASGYYTLARSDQQVKTWYSLNDGNW
                     NDYETWSLKPNEPDNPSHEIPGIMDRAVIQNNKTITVTTDDTQCGILDVIDGIVDFGT
                     TTGSTFSTITGQVNGKIRLAADNFPSGDAGEFADASSGGTVEYYGTGYNLSTSRTFRN
                     MVVNLTNTTNKLYLLSNYTLNGSLTITRGEFYFGDNVSTTSRSLTVYGDFVVEANGKV
                     HTGSANARYQLNLYGDFTNRGNIQLTNRAAADYTAEATDGIVDANFLSATRNQSMLLY
                     GPTRFYRIAISKGLSSTYELYMWASSSAYFELFGYANEGHPDEKQLLVNNNSLGLAYG
                     TVRVGANITVPRLNGGGNYNVSENAILWVDGGTVTKPSGTAVVVYGKVKVSAGTFNAN
                     INSGITTRLNGVFESTGGTTNLGQFRTSIYGTQHIGGFIQSGGYVTVDQAYGSSTDYY
                     LFSLSYEGNIFTLTGGTLHIKGTNAKGSVFINSDPVNQNVSTNATLILESTSTSPFRI
                     TSRAPFPNVTMSRSGSGTRTLVLEGGIVGSNPGNQAELPALPLVTKGTLTINDNVVFD
                     AKGQDVTIGRSYSIGSGASYIAASNTTTFQGASQNYLIAINAAATTKYFHNLTINNNG
                     YTGTLSVSDITVGNNLTITEGSLATGAKTVTVRGNIINSDTIKSTSGGILITNRGIVN
                     LVTVTNGGSYTSVPTVTIAAPGGSGIQATAVAIFNGTPSGSNPLPVARIAITNTGSGY
                     TSAPTVSFSAGAATATASISTTHELGGNGNGVFGSLELNEPHPSEASEKVEVTYLSAN
                     QSITDTLAITDGVLDIKAKSLTLDGYMGSETLTDYSPTKMIRTSGNHSDLGMSRRITS
                     NATYIYPLGIYTKTGDGSRYTPISQAFTSVSDTGYVQLNNVDNELPTLAPSAQSALQY
                     YWRVRHSGFSTIPEVYNIFYFYNLPDGYRPGMPDPATSWGTGKIVEITRYYNLGPLSW
                     DNPAKDLSLEYSHQESGQTGSSPPELQEGEFTTGHNTRFAGTVQVFYLRQNGNWRTNA
                     TWSFTRGGAPADDYPKQGDIAVIRRFSVGYNGIVEITNAETCAKVIFDDENGFSSGCP
                     RVQFSNSAAYGSNFAVVEVAETHQGGTPLDGELHGAVIKYDLYDGYAGEFPSGDFGDF
                     NRYPNALVIYTKEGASSEVTLSSSATEYPQVWFDPPANRTFILPDTLVTFNGMVIVPY
                     GHTLRMNTGSSAGATFKRKLNIGHSHDFGTGYFDFSGNASANQTVTAETNINLYYNAQ
                     LRINAPAGGGRTHLLKLHGNMTMETGCSVNLGDGNPANTNVTLELTGNGTNSLTGAGT
                     ASLTLSRVTMNKGTSQAQSFSFSWPFSLTGPTSGVTTDKALQLQNGTLYLNSAGIDIN
                     LSTGGGDFYIPSTSGLVVKNGKVNVSGNDNGILLDGLLRVETGGTINMDGGAGVNNYI
                     EYSASGNATLEVIGGTLTVGSQIRRGTTNPSGILKYTQTGGTVVVGKNAAPVANRGTF
                     EVLNTGSRFIHTGGLLP"
BASE COUNT         2500 a         2493 c         2772 g         2953 t
ORIGIN      
        1 aaaccttctg tccggcagct cttgcacgat gaacaggctc aagagagctg cccgccgaca
       61 tttcgggcga gtcctgcgct cagggagagg tgaacgcaaa aacctttgag tgctaccgga
      121 taaaaaccta ccttgaggcc ggttacaagc cctacatcat tgctgcaatg atgcttacca
      181 atcgtttcgc taatccctcc ctgttgatga gggcattgct cgaacggagg caggaacggg
      241 tactgcagac aggcaaacgg tagtaaaaag ttttacacgg tattaccatt cacctgtttt
      301 tcatggatca agtggaaaag ttggggtaat aatttagatc ccaagaaaca cccatgttat
      361 gagacatatg accataaccc ttctactcaa aaggggatga ataagacatt agggtttagg
      421 ttagatgagc ccttcggggg cacaactgga gattagtcaa tttttaatgt tgatttgcaa
      481 ccagctggga ctcaaaaatg tgagtataat cgtttggttc aggatttgaa ggatgatagg
      541 tttaatagga ttccaaagag tttggatgag ttgacatgca ggatagttgc cctagacgcc
      601 ttgttgggag cagaaggtaa cacgattgac cgtgtcggag tcgcagataa ccactgactt
      661 tcagctcggg gatagggaca atgaacaagg tggtgggctt tagccctgaa tatgcgccag
      721 aatagtgttt tcccggcatg ggtatcacca gggattgact tttacagtgt tgttaaaccc
      781 cgctacttct ggcgtaggtt aagagtcgaa tacgttggct agtaggcata gttattgggt
      841 atgtggtact ggggtccggt ttccggtatc ttgtacacgt caacatctac cccgggtcct
      901 accacagttc ctggtccaca gccggcatca tcagtaattg ctgtaacatt atagtggtaa
      961 acctttggta gtgcgggacc tgaccaggtt ggaagctgat cgggtgtggt gaagttgtag
     1021 gtataaggac ctgatcctga aagttcttcg acatcaacag ttatatctgg ccagcttccg
     1081 ccatggttat ccgcaacgga aaacgtaaat ggaggcacac cggttagggt gatttctatt
     1141 tctaccggtt caccctcgca catttgggat gggctaatca ggcctgtaac ttcggcttgt
     1201 ggggtttccg cgtagtcaac gaatgggctc ccgttcatcc attcataaca tgagctgtta
     1261 aggtcgttaa aagcacgtac ggtataggtt cccgactggt caactcctgt aaaggttaac
     1321 gctgaaccgg ttcctgccag ctcggtacca aagtaaactg cgtccctgta gagctggtag
     1381 gtgaatccga catccgaacc gttaagctca atggttgtgg ttgttggtcc gcaaatgtta
     1441 cctccgctgg ttacaatgta ttgggttggg ttggcatata catttacggt tggattagga
     1501 ccgtatatat agcctaatac cgaatcctta tcgcgtacgg tgtctatgac gtaggtgtag
     1561 gaaccggtta tcatgcccgt aatgtttatg gtgtgagggg atgaaaggat actatctaca
     1621 tgatagcttg tggtaccatc attatagtca atgaaccatg gtgaattacc ggtaagcgcg
     1681 atgactagct ggtaggtact cccctcgcat agtcccgcat cggaggatgt tatctgggct
     1741 gtaggaacaa caaccgattc aatggcgccc agggtaaaga actgctctgc gccattgaaa
     1801 gtaaagcttg ctgtggttga tatggttccg ctactagcgt ttccggttat ggtggcgcct
     1861 gtaccggcaa tttcccattt tgttccattc cagtaaacaa tgcgtaggtt gttaatatcc
     1921 gatattcctg aagctatgtc ggatgttcca tccagggtaa gctttattct agaggaaacg
     1981 ccattgggcc cagagattct ccagtactcg ctttggctaa ccgactgcag gggcgagact
     2041 aggctatcgt gccgcattgg cggtatatgg gctgaggggc tgctattgaa gtactcacct
     2101 gtccagtaac caacagctaa tggtaggtct aaggggtaga tgaatttttt tatgccgttc
     2161 ttgccgacag gtaaaaattg tttgggcgaa ttcgaattca ggtttatgga aaccgacccc
     2221 tcaaatagtc cattactata ggcgtagaac ccgtctccat ccgatttagc taccataaat
     2281 ggcttaccgg cagggttctg gatatgaccg ttattcatat acaggtaatt tctaatgccc
     2341 gacgaatcat tgaaggaaat gtttccggta ttgtcaaagt ggagggtatt gaattggtta
     2401 tcgctgttaa aaactccgat aaatgcctgg tcggtagtcc cattaacttt gaaggtttgg
     2461 ttggtgtagc tggtgaggaa tgaggtggag tcgtctctgg taaagttgcc ttcaaggttc
     2521 acccaagttc tgttgcccat atcccatgtg gccgaatcga gtaaataggt attcccttta
     2581 atagtggtgt agcggtaatc cgaaggaacc cctggctgtt taagggtagc actgctgtca
     2641 atcctcaagt cgccgcaaat ggtgatatcg tagttgggga atgtttttgt tccggtaccc
     2701 gtaattgtaa tattgttgta gctggtaatg gagctgggaa gcccataagt gcctcctgcc
     2761 cattccattg ttccccctgt accgcaggca aaaaaagtat cgtagctgcc ggacggaagc
     2821 gaactgatct ccgtggagag cgttccagta cccgatacgt aacccaaaat gtggtttttt
     2881 gttgagtcta gctccagccg gccattgatg gttgtccggt atgccctgcg gtagttactc
     2941 gacacggaca cagtatgcga ggtgcggata tggacccttt gaccaaccgg cccgccaacc
     3001 ggaactaacc caccatcctc ccgctcccat gtgctggagc ttgtccactc gccgttttgc
     3061 tttgaatagt agaggggaac ttcgttggga atatgcgatt cgatgccggc agtgtactcg
     3121 cccgttataa ggttaacgcc atcggttata aatgttataa aatcgtcgtc ttcatacacc
     3181 acctcaattg gcggaacctc gctttcctga aactttgccc agaagttatc gtagagtttt
     3241 gccccgatgt atgcgctttc atcgcccacc acgtcaccat taatgtagtg catacgcatc
     3301 agcgaggtga agttgctcag cccgctgctg ttcattatcc agtagtactg gagtacatcc
     3361 gtttgggttt cgtcaaaagt gatatgcttc ttgttaaccg ggaaaaggtt aacggtgccc
     3421 tgggtagcat ttgaagccac gtggagctca acaggggtgt attttttatc ggttccgttg
     3481 tcaacaccaa ttggaaaagt aaagctaaac gtataagctg gatcatctgg gtcgtagggg
     3541 tctgagggaa ccgcagaggg aactgttgga aaggcctttt caataccctg tatgtagtcg
     3601 ccgcctccca cggcaatcat tttggtacta tcaaaccccg atccctcaac agctgcgttg
     3661 agtcctaatg tcaacttgtt actctgtagc tgcaggatgc cgctagtcag ggccaggttg
     3721 ttctccatag ctatgctgtt ctcgagttga acgccgttgg ggttgtccac ctcaatgcga
     3781 ccgaactctc cattgccata tatcctttgt agtgaggttc cattaaatac tattccaccc
     3841 gttacgggat ttgagctgac gtgcgaggag ttgttggtaa cgttgccctt taccacaatc
     3901 ctgttggccc catcaaccag ctggcctgaa attatgtgca ggttggcatc aacctccagc
     3961 tccgatgaag gctgcaggct aaccgatgtc tcgggttcaa caattagatt tctagcccta
     4021 acgttgccgg tgatctgctg gttggctgag gatggagggg agctgcttcc ggccatgaac
     4081 agcgtatcaa cattcaaatt tgccattccg ttgttaacga tatgcctttt ggaggttagg
     4141 ttgaacgatc cggctccatc aaaggtgcag cccgaaccga tggtaaggtc gcccttaagg
     4201 gttaacgagc gatcctttag tcggagtgtt ggtgagttga cgccggcaat tgtcaggttg
     4261 cccagctcca tcgttgactt taaggtcagc acgcttgagg ctggcgtgtc gtcgctaccc
     4321 acctgaaggg tggtatttcc aatcgatgcc accgtgggtt cgagcagaat ggtggcctca
     4381 gtggcgctgg tctgagggcg agctacggta agagtccccc ggtatgaatg aatcggctac
     4441 ccgtatttaa aacctcaaag gttccccggt ttgccacagg agccgcattt ttgccaacaa
     4501 ccaccgtacc gcccgtttgc gtgtacttca gtatgcctga tggattggtg gttccccttc
     4561 tgatctggga gcccaccgta agggttccac cgataacctc aagtgtggca ttgcccgatg
     4621 ccgagtactc aatgtagttg ttaacccctg caccaccatc catgttaatg gttccaccgg
     4681 tttcaacccg gagcagcccg tcgaggagaa tgccgttatc atttccgcta acatttacct
     4741 taccattttt cacaaccagc cccgaggtgg agggaatgta gaaatcgcct ccacctgtgg
     4801 aaaggttaat atctatacct gcgctattca aatatagggt tccattttgc agctgaaggg
     4861 ccttatccgt ggtaactcca ctggtagggc cggtgaggga gaatggccag ctgaatgaaa
     4921 acgactgagc ctggcttgtc cccttattca tggtcactct cgacagggta agacttgccg
     4981 ttcctgcacc ggttagcgag ttggtgccgt tgccggttag ctccagtgtt acattcgtat
     5041 ttgcagggtt accgtctccc aggttaacag agcaacccgt ttccatagtc atgttgccat
     5101 gcagcttcag gaggtgtgtt ctgcccccgc cggctggggc attaatacga agctgggcgt
     5161 tgtagtaaag gttgatgttg gtttcggccg ttacggtttg gttggcgctt gcattgcctg
     5221 aaaaatcaaa gtaacccgtg ccaaaatcat gagaatgtcc aatgtttaac tttcttttga
     5281 aggttgcccc cgcacttgag ccagtattca ttcgtagggt atgcccgtag ggcacgatta
     5341 ccataccgtt aaaggtaaca agagtatcgg gcaggataaa tgttctgttg gctggcgggt
     5401 caaaccaaac ctgggggtac tccgttgccg aagaggaaag ggttacttca gatgatgctc
     5461 cttctttggt gtatattaca agagcgtttg ggtaccggtt aaaatcgcca aagtctcccg
     5521 aggggaattc tccggcgtac ccatcgtaaa gatcatactt gataacggct ccatgaagtt
     5581 ccccatccag aggtgtgccg ccctggtggg tttctgcaac ctctacaacg gcaaaattgc
     5641 ttccgtaggc cgctgagttg gagaactgta ccctggggca tccgcttgaa aagccattct
     5701 catcgtcaaa tatcaccttt gcgcatgtct cggcatttgt aatttcaaca ataccgttgt
     5761 atccgacaga gaacctccga attaccgcta tatccccttg tttggggtaa tcatcagcag
     5821 gggcaccccc gcgggtaaac gaccatgttg cattagtcct ccagtttcca ttttgacgta
     5881 ggtagaaaac ctgaaccgtt ccggcaaaac gtgtattatg ccccgtggta aattcccctt
     5941 cctggagttc cggcggtgag cttccggttt gcccgctctc ctggtgcgag tactccaggg
     6001 aaagatcttt ggccgggtta tcccaactca ggggacccag gttgtagtat cgggtgattt
     6061 ccactatctt tcctgttccc catgaggtag cggggtctgg cattccgggt ctatagccat
     6121 cagggagatt atagaagtaa aaaatgttgt aaacttcagg tatggtggaa aatcccgaat
     6181 gccgaactcg ccagtagtac tgcagagccg attgtgctga gggcgccagg gtgggcagct
     6241 cgttatccac attgttcagc tgcacgtagc cggtatcaga aaccgaggtg aaggcctggc
     6301 taatgggggt ataacggctt ccatcgccag tttttgtgta tattccaaga ggatatatgt
     6361 aggttgcgtt tgaggttata cgcctcgaca ttcctaaatc ggagtggttg cccgaggtac
     6421 ggatcatttt ggtagggctg taatctgtaa gggtttcgct acccatatac ccgtcaagcg
     6481 tcaggctctt tgcctttatg tcgagtacgc catccgttat tgctagcgta tcggtaatag
     6541 attgatttgc agacaggtat gttacctcca ccttttcgga tgcttcgctt gggtggggtt
     6601 cgttcagctc caggctgcca aatacgccgt tgccgttgcc accaagctca tgggttgttg
     6661 agatggatgc cgtggcagtg gctgccccag cactgaagct caccgttggg gctgaggtgt
     6721 acccgctacc ggtgttggtt atggctattc tggccactgg gagcgggtta gatccagagg
     6781 gagttccgtt gaatatggcc acagcagtgg cctggattcc cgatccgccg ggagctgcaa
     6841 tggttaccgt tggaacggag gtgtagctgc ccccattagt aaccgtaacc agattcacaa
     6901 taccacggtt tgtgataaga atacctcctg atgtgctttt aatcgtgtcg gagttaatga
     6961 tattgccgcg cacagttact gttttagcgc cggttgcaag cgacccttcg gtaatggtca
     7021 aattgttacc aactgtgatg tcgcttaccg aaagggttcc ggtgtaacca ttgttgttta
     7081 ttgtgaggtt atggaagtac tttgtagttg cagccgcatt tatggcaata aggtagttct
     7141 gggatgcccc ctggaaggtt gtcgtgttac ttgccgcaat gtagctggct cccgaaccaa
     7201 tgctgtagct ccgcccaata gttacgtctt gtcccttggc atcgaatact acgttgtcgt
     7261 ttattgtcag agttcccttt gttaccaagg gcagagccgg cagctcagct tggttaccgg
     7321 gattgcttcc tacaattccc ccctcaagca ccaaggttct tgttcctgag ccggaacggc
     7381 tcatggttac atttggaaag ggagctcgtg aggtaatgcg gaaaggggaa gtgctcgtgg
     7441 attctaaaat cagggtggcg ttggtgctaa cattctggtt tacagggtcg gaattgataa
     7501 aaaccgaacc cttggcgttt gttcccttga tgtgcagcgt tccaccggtc agggtgaata
     7561 tgtttccctc ataggatagc gagaagaggt agtagtcggt ggatgatccg taggcctggt
     7621 caacggttac atagcctccg gactgaatga atccgccaat gtgctgggtt ccataaatgg
     7681 atgttctgaa ctggcccagg ttggtggtgc caccggtgga ctcaaacacg ccgtttaacc
     7741 tggtggtgat tccgctgttt atgttagcgt tgaacgttcc tgccgaaacc tttaccttgc
     7801 cataaactac cacagcggtt ccgcttggtt tcgtaactgt gccgccatca acccaaagga
     7861 tggcattttc cgaaacgttg tagttgcctc ctccattcaa acggggcacc gttatatttg
     7921 ccccaaccct tacggttccg taagccaggc ctaacgagtt gttgttaacc aacagctgct
     7981 tttcgtcggg gtgcccctcg ttggcatagc caaaaagctc gaagtaggct gagctggatg
     8041 cccacatgta gagttcgtag gtgctggaga gtcccttgct gattgctata cggtagaaac
     8101 gggttgggcc gtatagcaac atgctctggt tacgcgtggc gctcaggaag ttggcatcaa
     8161 caatcccgtc ggttgcctcg gctgtgtagt ctgccgcagc acggttggta agttgaatgt
     8221 taccacggtt ggtaaaatcg ccataaaggt taagctggta ccgcgcattt gcggaacccg
     8281 tatgaacttt cccattggcc tcaaccacaa aatccccgta aacggttagc gagcgggagg
     8341 ttgtggagac gttatcacca aaataaaact cacccctggt aatggtcaac gagccgttta
     8401 gggtgtagtt cgacagcaag tagagcttat tggtggtgtt ggtcaggttt acaaccatat
     8461 tccggaaggt tcgggaggtt gaaaggttgt atcctgtccc gtagtactcc acggttccgc
     8521 ccgaggaggc atcggcaaat tcaccggcat cgcccgatgg aaaattatcg gctgccagcc
     8581 ttatctttcc gtttacctgg ccggtaatgg ttgagaacgt actgccggtt gtggtaccaa
     8641 aatctacaat cccgtcaatc acatcaagaa ttccgcactg ggtatcatct gtagtaacag
     8701 taatggtttt attgttctgt ataacggctc tatccatgat tccaggaatt tcatgcgagg
     8761 ggttatcagg ctcgttgggt tttaggctcc atgtttcgta atcgttccag ttcccgtcgt
     8821 ttagcgagta ccatgttttt acctgctggt cgcttcttgc cagggtatag tatccgctgg
     8881 caaaattaga gctgctcaga ttgatagaaa cctttccgtt ggaggcaacc ccggttccac
     8941 cagggacaga ggagaaggtt ccggatgttc ccgatcggta gagcaggtag taaaccctgt
     9001 cggtcgaaag ggatagtccg gcctcggtaa agtcgaaggt aatccgtaca tcggtgcttc
     9061 cagcatcaat aactccattc tggacccgct caacgtagta aatcctatcc caacgctgat
     9121 caaggttaac accgggaatg gttggtaggt cgttggtatt gaagccatgc ggtgtgccat
     9181 cgtgtccggc aaaaacaaac tcattggttt catcgagcgt tccattgcgt tcggacagga
     9241 gcaagccgga gccaacattt ctggtttggc tgtacctatc gccacccgaa tgaccaatcc
     9301 caataaaatc ttgcgtgtgc gtggttgaag agtaggaggt tggcgttatg gcaatgttgt
     9361 aacgattagc caggtaagcc tctacaatta acctttgcgc cttattaagg ttgtctctgt
     9421 acacaataat ctccgaaata tcgccgttaa atgcctgagc atatccgggg ttaagggttc
     9481 caatgtgcag gtcggaggcc atatttgcaa tgctaaccgg cccgtttccg ttgccgcttt
     9541 cctcaccatt gtaaaagatt tttgaacgtg ggttagccac cgatccatcg taaatggccg
     9601 aaaatatctg gggttggtct gttacaggat cgttgccgtt gattcggtaa gttgaggcgt
     9661 taaaatatag gttcctaccg gtgtacgtga ataggtagaa ggcctcctca tttccggcgc
     9721 ttactctttt ggaaaccagt cctcttgcca aagcatccgg actaacgggt tgggcaataa
     9781 caaaaacgct taccccattg gtattgtcaa ggttgtcggc atcggcaacg gtcaaatggt
     9841 catttgttcc atcaaatcga attgtaggca agccgttaat tcccgaagaa atgtaaatgg
     9901 gctggttgcc cactgttccc tgagcggcat ggtttgcatt cccgctaata tcattccagc
     9961 cactcactgg ttctccgttt gtggtggttg aggttccctg atcggagcgc agccacagca
    10021 cattttcgct gcttgtgcct atgcctgccg gagcgtactg ggccattcct tcaactgaga
    10081 ggaaaaacag taagcctaaa aggcccacta tccttatgtt gatgccttgt ggattattgg
    10141 acagaggtgc attcatcttg tggctatttt atagctatca tacaaaattg caatatttac
    10201 gccaaattta ataaaaataa gtatatcagt tagttattct agaaaaggaa aaatagcaga
    10261 ccaattagtg tctcaaaacg ggcattattg tgagctgtta tgtaaaaata tacaaactaa
    10321 atgtgaaaag ttaaagggaa gaagattaaa gaattgactt tttgtctaat ttccgcaggt
    10381 gaaggatttt atcttcactt caaagcaact tcatccctgt ctttatccct tttttgtccc
    10441 agcgctttgg aaaattccct gcgttgcgat gcagctggtc agggttgcgc tttggaagat
    10501 ttgctacttg catcgccaat tcttcaccta ccgcgtcata ccccgggctt gcgcctgggg
    10561 ttaatcatgt ttcgcccctt ctgggcttaa aacacatcct gaaattgttc tgaatcttgg
    10621 atttttttcc ctgagcttgt cgaagtttga acctataacc tttatgtcta gtcctaaaat
    10681 atgtctacag gttaataaat aaaattaagc aacacttt
//
LOCUS       DAOSHM010000076        10444 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_5396_length_10444_cov_20.865531, whole genome shotgun
            sequence.
ACCESSION   DAOSHM010000076 DAOSHM010000000
VERSION     DAOSHM010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 10444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10444
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5396_length_10444_cov_20.865531"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="PK839_09390"
     CDS             <1..191
                     /locus_tag="PK839_09390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72989.1"
                     /translation="LTPEVAAYIERRLSHSMAARADFRMSEAGGHWLACPPLPCLTKE
                     LHLQLKSTFHKIVKVRKN"
     gene            328..1194
                     /gene="panC"
                     /locus_tag="PK839_09395"
     CDS             328..1194
                     /gene="panC"
                     /locus_tag="PK839_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HOG72990.1"
                     /translation="MKTIHTLKTCSEVEQSLWSTRENGKSIGFVPTMGALHGGHISLV
                     ERAASENDVVVVSIFVNPTQFNNPNDLVSYPRTPEKDLELLEKTGVDYVFMPSETEIY
                     PQPDTRTFNFGQLDKVMEGAHRPGHFNGVAQVVSRLFDIVKPNRAYFGQKDYQQLVII
                     RQLVRMLGLSIDIIACPIVREADGLAMSSRNLLLSPECRASAPLIHRTLVEACNQIEQ
                     MNVKDMIGWVEARVNQDKNLRVEYFEISNAETLEPVSSWTDAENIIGCIAVWAGSVRL
                     IDNIFLKHSTKG"
     gene            1197..1547
                     /locus_tag="PK839_09400"
     CDS             1197..1547
                     /locus_tag="PK839_09400"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HOG72991.1"
                     /translation="MLIEVVKSKIHRVTITEANLNYIGSITIDEDLMDAANLIENEKV
                     QVVNVNNGERLDTYIIKGERGSGQVCLNGPAARKCVTGDVVIIMSYATMDFEEAKHFS
                     PTVVFPDTATNKLV"
     gene            1565..2584
                     /locus_tag="PK839_09405"
     CDS             1565..2584
                     /locus_tag="PK839_09405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOG72992.1"
                     /translation="MKVFPSRRIVNFFIFFLLSILLLYLAFRKVDFSHVAQGFRNVSY
                     LWIFLSLLVGLASHALRAVRWSILVEPLGKKPSFLNMFSAVMVGYIANLALPRVGEVA
                     KCGSVSRTDGIRFESLVGTVVVERAMDLVMLLLATITVVALKFDFFGKFIISRIFHPL
                     RDRALQLNGYQLAAVLVVVVAFILFVRLILRSEIFGSKISLKIRSIWNGTVDGVKSIA
                     HTNKLGQFLVLSVVMWLCYWVMTWLLLYSTPITANLTVWDGLFIMVIGSYGMVVPVQG
                     GFGAYHIITAIALGIFGITYDDGLVFAIISHESQTFLVILGGILALIYVYIIQRPDKP
                     KPQSL"
     gene            2826..3302
                     /locus_tag="PK839_09410"
     CDS             2826..3302
                     /locus_tag="PK839_09410"
                     /inference="COORDINATES: protein motif:HMM:NF013621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HOG72993.1"
                     /translation="MNSEVIEKKILEDVELDRMLAYWKFRDRSVVALYGSFDVLHPGT
                     FELITYAAEHGHELVVGIKPDGEMKKEKGDKFPVFGQNKRALVVASNQLVSAVYICQN
                     GIEDFLRRVKPSLAVCCHHASSTDKDKLEVVKEWGGKYKIFTQSTTSVEDIITKLC"
     gene            3501..4898
                     /gene="radA"
                     /locus_tag="PK839_09415"
     CDS             3501..4898
                     /gene="radA"
                     /locus_tag="PK839_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HOG72994.1"
                     /translation="MAKTKSVYVCQNCGAESVKWLGRCPSCGEWNTYVEERITKETSS
                     RPGLVNVSRNAIPTPIREIDSDNELRVDTKIGELNRILGGGIVPGSVILLGGEPGIGK
                     STLALQLALGLKSQKVLYISGEESARQVKLRADRINHDSQDCLILNETLLENILTQTE
                     RVCPELVVIDSIQTLYTDTIESSPGSVSQVRETAAALLRYAKTSGTPVILIGHITKDG
                     SIAGPKVLEHIVDVVLQFEGDQNYLYRILRSAKNRFGSTSEIGIFEMQSGGLVEVTNP
                     SEILLSHREEMLSGIAVAATIDGARPFLIETQALVSTAVYGTPQRSTTGFDPRRLNML
                     LAVLERRAGFRLANKDVFLNIAGGLRVNDPAIDLAIISAILSSTFDVAIAGSTCFAAE
                     VGLSGEIRPVSRLDQRIAEAAKLGMSRAFVSKYNLKKSDVHPKGIEVIPLGRIEELVK
                     ALFGRSSGRQQQADS"
     gene            4925..6196
                     /locus_tag="PK839_09420"
     CDS             4925..6196
                     /locus_tag="PK839_09420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG72995.1"
                     /translation="MRIAIIGFGAAAIGIMERLKESQHEIHVFEKSADIYSSSISGIR
                     ADGKLFVSSEMGGDITIDTALQRKLVDYYINFTEKKNVETGTSFGSSRFYKVFYDRGF
                     LPVTSEFFHIGTDQLKEVLFNIYTHLSGVKNIHFHFNSTVSSIKIEKEGVLLNGTEHF
                     DMAVVTVGRSGHKLINRLIEDYPYLVKNNTKVDLGIRFELPNHIVEELNHEMYEFKVK
                     YKSRTGYMVRTFCNNPSGFVVTENYDDFTTVNGHAKLHEKSSNTNFAILASLILTEPF
                     NDPIGYGSNIAKLSNLLAGEGKVILQTYSHFKESKRTKNLYRVSPTLAADKFILGDIN
                     LAYPRRIIESIIDFIENLNTVIPGVANNDNLVYAPEIKFYSNRLNNLANPFIKFAGDC
                     SGATRSIIYATAHGYMMGEAILNNNFSTLES"
     gene            6196..7806
                     /locus_tag="PK839_09425"
     CDS             6196..7806
                     /locus_tag="PK839_09425"
                     /inference="COORDINATES: protein motif:HMM:NF008453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG72996.1"
                     /translation="MLKVDNLSVAFGQSMAVNGINFSLEGNQILGIVGESGSGKSVTS
                     LSIMGLLPKNARITNGSIWFEPPGCIPVNLVGINPEQHRQLRGKSIAMIFQEPHTCLN
                     PSMRCGVQLVEALELHAGLKGKPAVKQCLSLLSEMQLPDPETISRSYPHQLSGGQKQR
                     VMIAMALAGKPKLLIADEPTTALDVTVQKGILELLLSLRNRYGMGIIFISHDLSVVEQ
                     VADRVLVMRNGMMVEEGKAEDILQKPQHPYTRELIGFRREIYSPGNSSKTRNEGGGVV
                     FSVNSLTVRYEKSNRLSAGKAKNQIRAVSNVSFSIHEGETLGLVGESGSGKSTIGRTL
                     LRLIGNYSGQIAYKGKPLNGFTTQQLKEFRREVQLIFQDPYSSLNPRLTIGQALVEPV
                     VVHGLAGKNEAKSRVLELLDRVKLPADSFYRFPHEFSGGQRQRLVIARALTLQPKVLI
                     CDEIISALDIAIQVQILNLLEELKRDFNLTYLFISHDLGVVRHISNRVIVLKEGTIDE
                     EGPAEAIFAQPKSTYTKTLIAAMPQLLA"
     gene            complement(7859..10204)
                     /locus_tag="PK839_09430"
     CDS             complement(7859..10204)
                     /locus_tag="PK839_09430"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HOG72997.1"
                     /translation="MNWKNPEVIRKFKWWFWGIISFLLLFFLVIFILINLEFFGPMPT
                     FEELENPKSNIASEIISEDNVVLGNFYVQNRTFVGYDEISPNVIHALIATEDNRFYSH
                     SGIDAKGLGRVMVKTILFGKKTAGGGSTISQQLAKNLFPRDTSGRKNALVRTTKLVLS
                     KFKEWVTAVKLERNYTKEEILAMYLNVVEFGSNSYGIKSAAKTFFNTTPDSLHVEQAA
                     LLVGLVNAPTRYSPLRNPDKSLQRRNLVLQQMAKYGYIDRNQLDSLVAIPITLNYMEQ
                     DHNAGLATYFREMLRQFMTARKPERRFYYSQDMYSQDSLLWETNALFGWCSKNLKPDG
                     TPHNIYRDGLKIYTTINSKMQEYAEKSLTDHLKFELQPQLDREIKSRGGVLFSSQITR
                     EQANQIMYSAMRQTERYRLLRNAGASNESIIENFNTPVPMRVFSWRGERDTVLSPLDS
                     IKYYKKFLRSSFMAINPHDGHVKAYVGGPDFRHFKYDMVRQGKRQVGSTIKPFLYTLA
                     MQEGYSPCMEVPNVPQTFVVGDTTWNPKNSGSTKYDGQMVTLKWGLANSINNISAWVM
                     KQFTPQIVADMINQLGIKTHVDPVPSIFLGTIDFSLFEMVGAYGTFANKGIFIDPIFV
                     TRIEDRNGNVLATFNTVKTEAISEQTAYLMINLLEAVVNQGTGIRIRYKYQLPGKIAG
                     KTGTSQNHSDGWFMGVTPNLVAGVWTGAEDRAVHFDNISMGQGASMALPIFATFLQKV
                     YANPSLGVSPNDDWDKPILKQNVNLDCNKQFDNEINEIELY"
BASE COUNT         2810 a         2454 c         2542 g         2638 t
ORIGIN      
        1 tgctcacccc cgaggtagca gcctacattg agcggcggct tagccattcg atggctgccc
       61 gagcagactt cagaatgagc gaagctggag ggcattggct tgcatgtcca ccactaccat
      121 gtttaacaaa agagttgcat ttgcagctta aatctacctt ccataaaatt gttaaggtac
      181 gaaaaaattg aaaaaggaat attcttttct gttgatttcc aacaagttgt tttgcttctt
      241 ttaaaggaaa gatgtgccgg aatagtttgg agaagcaaaa aaaatggcaa actttgctgc
      301 cggaattcac caaagttgac caatcaaatg aaaacaatcc atacgctaaa gacctgttct
      361 gaagttgagc agagcctttg gagcaccagg gaaaatggca aatcaatcgg gtttgtacca
      421 accatggggg cgcttcatgg gggccacata tcgctggtgg aacgcgcagc ctccgaaaat
      481 gatgtggtgg tggtaagcat tttcgtcaac cccacgcagt tcaacaatcc aaacgacctg
      541 gtaagctacc cgcgaactcc agaaaaggat ttagaactac ttgagaaaac gggggttgat
      601 tacgtgttta tgccctccga gactgaaatc tacccccaac ccgatacccg cacgtttaac
      661 ttcggacaac tcgataaggt gatggagggc gctcacaggc ctggccactt caatggagtt
      721 gcacaggtgg ttagcaggct tttcgatatt gtgaagccaa accgggctta ctttgggcaa
      781 aaggattacc agcagctggt catcatcagg cagctggtta gaatgctggg actcagcatt
      841 gacattattg catgccccat agtccgtgaa gccgacggct tggccatgag cagtcgtaac
      901 cttcttctgt caccagaatg cagggctagc gcaccgttaa ttcacagaac ccttgttgag
      961 gcctgtaacc aaattgagca aatgaacgtt aaagatatga ttgggtgggt agaagcacgg
     1021 gtaaaccagg ataaaaacct tagggtggag tactttgaga tttcaaatgc cgaaacactg
     1081 gagcccgtaa gcagctggac tgacgctgag aacattattg gatgtattgc tgtttgggcg
     1141 ggtagcgtta ggctgataga taacattttt ctcaaacact caacaaaggg gtagctatgt
     1201 tgatagaagt agtaaaatcg aaaattcacc gggtaaccat aaccgaggca aacctgaact
     1261 atattgggag cataaccatt gatgaggact taatggatgc tgcaaacctg atcgaaaacg
     1321 aaaaggtaca ggtggtaaac gtgaacaacg gggagcggct cgacacgtat attattaaag
     1381 gcgaaagggg atcggggcag gtgtgcctga acggccctgc ggcccgcaag tgcgttaccg
     1441 gcgatgtggt tatcattatg tcgtacgcca ccatggattt tgaggaagcc aagcactttt
     1501 ctcctaccgt tgtgtttccc gatactgcaa ctaataagct tgtttaacac ttaacccgtt
     1561 cgccttgaaa gttttccctt cccgtcgaat cgttaacttt ttcatcttct ttctactctc
     1621 catattgctg ctctaccttg ccttccgcaa ggttgacttt tcccatgttg cacagggatt
     1681 tagaaacgta agctacttat ggattttcct ttcgctactg gttggattgg catcccatgc
     1741 gctacgggct gtccgttgga gcattcttgt tgaacctctt gggaaaaaac cttcattcct
     1801 gaacatgttt agcgccgtga tggtggggta tattgcaaac ctagcactcc cacgggtagg
     1861 ggaagtagca aaatgcggaa gtgttagcag aaccgatggt attcgctttg aatcgttggt
     1921 tggaacggtg gtggtggaac gcgcaatgga cctggtaatg ctgctgctag caacgattac
     1981 ggtggtagcg ttgaaatttg atttttttgg aaagtttatc atttcacgaa tttttcaccc
     2041 gttaagggat cgggcgctcc agctcaacgg ttaccagctg gctgcagttc tggttgttgt
     2101 ggttgccttc atacttttcg tcaggctaat tctacggagt gaaatttttg gcagtaaaat
     2161 cagcctcaaa attcgatcca tctggaacgg caccgttgat ggagttaaat caatagccca
     2221 caccaacaag ctgggccaat tccttgtact ttcggtagtc atgtggcttt gctactgggt
     2281 aatgacatgg ttgctgcttt actcaacccc cataactgca aatcttaccg tatgggacgg
     2341 tctattcata atggttatag gctcgtatgg catggtagtt ccagtacagg gaggatttgg
     2401 agcataccat atcattaccg ccattgccct tggtatcttt ggaattacct acgacgacgg
     2461 gttggtgttt gccattattt cacacgaatc acaaaccttt cttgttatac tgggcggcat
     2521 cctggcctta atctacgttt acataattca aaggccggac aaaccaaaac cccaatccct
     2581 gtaaagtcat tagtaggcaa gaagcttata gcaactcgat gccaggttga ctattagcat
     2641 tactttacct ttactggcga tgctaaactt ttttgaaaat ccaatttttt agagaatcct
     2701 gctccccctg accatagcag attttaatat acctaaaact aacgtcatca tgctaaaatt
     2761 tgctatgttt gtacaaatac aacattggct aagagtgaat catacacagc taaaaccata
     2821 cccagatgaa cagcgaagtg atagaaaaga aaatccttga ggatgtggag cttgaccgta
     2881 tgcttgccta ctggaagttt agagatagaa gcgtggtggc cctttacggg agttttgacg
     2941 tgcttcatcc cggaacattt gagcttatta cctatgctgc cgagcacggg catgagctgg
     3001 ttgttggcat taaacccgat ggggagatga aaaaggaaaa aggggataag ttccctgttt
     3061 tcgggcagaa caaaagagcg ttggtagtag cctccaacca gctggtttct gcagtttata
     3121 tttgccaaaa cggcattgaa gattttctcc gaagggtaaa accttcgctt gcagtatgtt
     3181 gccatcatgc atcctccacc gataaggata aacttgaagt agtaaaggag tggggtggta
     3241 agtacaaaat tttcacacaa agcaccacca gcgttgagga tatcatcacc aagctatgct
     3301 agattgtaaa cgtggcatag cacccattca tggtaagcca gcttttgcgt ggcggatggg
     3361 gaattggtat ttaagatgga tattggactt ataggtgcat gatatagcta tcattaaatc
     3421 tactttttag gcgctagctc ttcaacattg ttaacaatta attgcttgcg agataacgtg
     3481 cgctataagc taggcgagta atggcaaaga caaaaagtgt ttacgtttgt cagaactgcg
     3541 gggcagaatc ggtgaagtgg cttggccgct gcccctcatg tggagagtgg aatacctatg
     3601 tggaggaaag aattaccaag gaaacctcca gcaggccagg gctggtaaac gtatcacgga
     3661 acgctatccc tactcccatc agggaaattg attcggacaa cgagttacgg gttgacacca
     3721 aaattggaga gctaaaccgc atactgggcg ggggaattgt acccggttcg gttattctgc
     3781 tgggcgggga gcctggtatt ggaaaatcaa cactcgccct tcagctggca ctaggcctaa
     3841 aaagtcaaaa agtgctctac atatcggggg aggaaagcgc ccgccaggtt aagctacgcg
     3901 ccgaccgtat taatcacgac agccaggact gccttatact caacgaaacg ctgctggaaa
     3961 atatactcac ccaaaccgaa agggtatgcc ccgagttggt ggtaatagac tccattcaaa
     4021 cactctacac cgataccatt gaatcgtcgc caggcagtgt ttcgcaggtg cgtgaaacgg
     4081 cggctgccct gctcagatat gcaaaaacat cggggactcc ggttatcctt ataggccaca
     4141 tcaccaagga tgggagcatt gccggcccta aggtactgga gcatattgtg gatgtggtgc
     4201 tgcagtttga aggcgaccag aactaccttt accgcattct ccgctcggcc aaaaatcgtt
     4261 ttgggtcaac ctccgaaatt ggaatattcg aaatgcagag tggtggactt gttgaagtta
     4321 ccaacccatc ggaaattttg ctgtcgcacc gcgaggaaat gcttagcggc attgcggttg
     4381 cagctaccat cgacggggca aggcctttcc taattgaaac ccaggccctg gttagcacgg
     4441 cagtttacgg aaccccccaa cgttcaacca caggtttcga cccccgcagg ttgaacatgc
     4501 tgctggctgt gctggagcgc cgggcaggtt tcaggcttgc taacaaggat gtttttctga
     4561 acattgccgg tggacttagg gttaacgatc ctgccataga cctggccatc atatcggcca
     4621 ttctctcctc caccttcgat gtggcaattg ccggttcaac ctgtttcgcc gctgaggtag
     4681 gcctgtcagg cgagataagg ccggttagcc gtctcgacca gagaattgcc gaggctgcca
     4741 agctgggcat gagcagggct tttgtatcaa agtataacct taagaaatcc gatgtccacc
     4801 ccaagggaat agaggttatt cctttaggac gaattgaaga gctggttaag gctctttttg
     4861 gccgatcgtc aggccgtcag cagcaggctg atagctaaac atgttgaaat taaattttta
     4921 agccatgagg atagccatta tcgggtttgg tgctgctgcc atcggcatta tggaaaggct
     4981 gaaggagagc cagcacgaaa tccacgtgtt tgagaaaagc gccgatatct actcatctag
     5041 catttcgggc atcagggcag atggaaagct tttcgtctca tccgaaatgg gaggcgacat
     5101 aaccattgac accgcccttc agcgtaagct ggttgattac tacatcaact ttaccgagaa
     5161 aaaaaatgtt gaaaccggta cttcatttgg ctcgtcaaga ttctataaag tattctacga
     5221 caggggattt ttgcccgtta cctcggagtt tttccatatt ggcaccgacc agctcaagga
     5281 ggtgctattc aacatttaca cccatcttag cggggtaaag aatatccact tccacttcaa
     5341 cagcacggta agcagcatta aaattgaaaa ggagggcgta ctcctgaacg gaaccgaaca
     5401 cttcgacatg gcggttgtaa cggtgggccg aagcggtcac aagctcatta acaggctgat
     5461 tgaggattac ccctacctgg taaagaacaa caccaaggtt gatttgggca tacgttttga
     5521 gctgcccaac cacattgttg aggagctaaa tcacgagatg tacgagttta aggtgaagta
     5581 caagagccga acaggctaca tggtaagaac tttttgcaac aatccctcgg ggtttgtggt
     5641 aaccgaaaac tacgacgatt tcacaactgt aaatggccat gccaaactgc acgagaagag
     5701 cagcaacacc aacttcgcca ttctcgcctc gctcatccta accgaaccat tcaacgaccc
     5761 cataggctac ggatcgaaca tcgccaagct ctcgaacctg cttgccggcg aaggtaaggt
     5821 gatactgcaa acctacagcc attttaagga gtcgaaacgc acaaaaaacc tctacagggt
     5881 gtctcctacg ctggcagcag ataagtttat cctgggcgac atcaacctgg cctacccccg
     5941 acggataatt gagagcatta ttgacttcat agaaaatctc aatacggtca ttccgggagt
     6001 agccaacaac gataacctgg tgtatgcgcc cgaaattaaa ttttactcca accggcttaa
     6061 caacctggct aacccattta taaagtttgc cggtgactgc tcgggcgcta cccgatccat
     6121 tatttacgcc actgctcatg gatacatgat gggtgaggcc attctgaaca acaacttctc
     6181 aaccctcgaa tcttaatgct gaaggttgac aacctgagcg ttgcctttgg gcaaagcatg
     6241 gccgtgaacg gtatcaactt ctccctggag ggcaaccaga ttcttggaat tgttggggaa
     6301 tcgggttcgg gcaaatcggt tacctcgctc tccatcatgg ggctgctgcc caaaaatgcc
     6361 cgcattacaa acggaagcat ttggtttgag ccacccggct gcattcctgt aaacctggtt
     6421 ggaattaatc ccgaacagca ccgacagcta aggggcaagt cgattgccat gatatttcag
     6481 gagccccaca cctgcctcaa tccctccatg cgctgtgggg ttcagctagt ggaggccctt
     6541 gaacttcatg ccggtttgaa ggggaaaccg gccgtgaagc aatgcctttc gctgctctcg
     6601 gaaatgcagc tacccgatcc cgaaacaatt tctcgaagct acccccacca gcttagtgga
     6661 ggacaaaagc aaagggtgat gattgccatg gccctggcag gcaaacctaa actacttata
     6721 gccgacgagc caacaaccgc tctcgacgtt accgttcaaa agggaatcct tgaactttta
     6781 ctaagcctgc gcaaccggta tggcatgggt ataattttca tctcgcacga tttaagcgtg
     6841 gttgaacagg ttgccgacag ggttctggta atgagaaacg gcatgatggt tgaggaagga
     6901 aaagctgagg acatcctgca gaagccacaa catccctaca ccagggagct gattggtttc
     6961 cgtagagaaa tttactctcc cggaaattct tctaaaacga ggaatgaagg aggtggcgta
     7021 gtattctcgg ttaactcgct aactgttcga tacgaaaaga gtaacaggct atcggcaggt
     7081 aaggcgaaaa accagattag ggcggtaagc aatgtgagct tctccattca tgaaggcgaa
     7141 acattggggc tggtgggcga gtcggggtcg ggtaaaagca ccattggccg aacacttctt
     7201 aggctaattg gaaactactc cggccaaata gcttacaagg gtaaaccact taacggcttc
     7261 accacacagc agctaaagga atttcgccgg gaggttcagc tgatttttca ggatccctac
     7321 tcctcgttaa acccacgctt aacaattgga caagctcttg tagaacctgt tgtagtccat
     7381 gggctggctg gcaagaacga agcaaaatca cgcgtgctgg agttgctgga cagggtgaag
     7441 ctacctgctg atagcttcta ccgttttcca cacgaattct caggtggtca gagacaacgg
     7501 ctggtaattg cccgcgcgct tacccttcaa ccaaaggtgt tgatttgcga tgagattatt
     7561 tccgcccttg acattgccat acaggttcaa attctgaacc tcctcgaaga gctaaaacgg
     7621 gacttcaacc taacctacct tttcatctcg cacgatttgg gggtagttag gcatatcagc
     7681 aaccgggtga tagtcctaaa ggagggaaca attgacgagg aaggccctgc tgaggcgatt
     7741 tttgcacagc ccaaaagcac ctacacaaaa acgctcattg cggccatgcc gcagctacta
     7801 gcctgatgta acccgaaacg atcaagccca aacctgctac catgcacgaa gcaaaaggct
     7861 agtacagctc aatttcgtta atctcattat caaactgctt attgcagtcg aggttaacgt
     7921 tttgctttag gatgggttta tcccaatcat cgttgggcga aactccaagc gatggatttg
     7981 catatacctt ctgcaaaaaa gtagcaaaaa ttggcaaagc catgctggcg ccctgcccca
     8041 tggaaatatt gtcaaaatga acagccctgt cctcggcgcc ggtccaaacg ccagcaacca
     8101 ggttgggagt aacacccatg aaccatccgt cggagtgatt ttgggatgtc cccgttttcc
     8161 cggcgatttt tccgggcagc tggtacttgt agcgtatgcg aataccggtg ccctggttaa
     8221 caacagcttc gagcaggttg atcatcaggt aggctgtttg ctcgcttata gcctcggttt
     8281 tcaccgtgtt aaaggtagcc agcacatttc cattcctatc ctcgatgcgg gttacaaaaa
     8341 ttggatcgat aaaaatgccc ttattggcaa aagtgccgta ggcccccacc atctcgaaaa
     8401 ggctaaagtc aatggtgcca agaaaaattg aaggaaccgg atcaacgtga gttttaatac
     8461 ccagctggtt aatcatgtct gcaactattt gcggggtaaa ctgcttcata acccaggccg
     8521 aaatgttgtt gatggagttg gccaaacccc acttaagggt taccatctgc ccgtcgtact
     8581 tagtactgcc ggaatttttg gggttccatg ttgtatcgcc taccacaaaa gtttggggaa
     8641 catttggaac ctccatgcat ggggaatacc cctcctgcat ggcaagtgtg taaagaaaag
     8701 gcttaatggt ggagccaacc tgtcttttcc cctgcctaac catgtcgtac ttaaagtgtc
     8761 gaaaatcagg gccacccacg tatgccttca catgaccatc gtgggggtta atggccataa
     8821 aactcgaccg aagaaacttt ttgtagtact tgatggagtc gaggggtgat aaaaccgtat
     8881 cgcgctcacc ccgccatgag aaaaccctca tcggaacggg ggtgttgaag ttttcgataa
     8941 tactctcgtt tgaagctccg gcattacgaa gcagcctata acgctcggtc tgcctcatgg
     9001 ctgagtacat aatttggttg gcctgctcac gggtaatctg tgaggaaaac agaaccccac
     9061 ccctactttt tatttcacga tcgagctggg gttgcagctc aaacttcagg tggtcggtaa
     9121 gcgacttttc tgcgtactcc tgcattttgg agttgatggt ggtgtaaatt ttaaggccat
     9181 cacggtagat gttgtggggt gttccatcgg gcttaaggtt tttggagcac catccgaaaa
     9241 gggcattggt ttcccaaagt agggaatcct ggctgtacat atcctgagag tagtaaaatc
     9301 gcctttcggg tttacgggcg gtcataaact gcctgagcat ttccctgaag tacgtcgcca
     9361 aacccgcatt gtggtcctgc tccatgtagt tcaaggtgat tggaattgca accagggaat
     9421 cgagctggtt gcgatcaatg tacccatact ttgccatctg ctgcagcacc aggtttcgtc
     9481 gctgtaacga cttatcggga ttccgcaggg ggctataccg ggttggagca tttaccaatc
     9541 ccaccagcag ggctgcttgc tcaacatgaa gagaatcagg agtggtgttg aaaaatgttt
     9601 tagctgccga tttaatccca taggagttgc tgccaaactc aaccacgttc aggtacatgg
     9661 ccagaatttc ctccttggtg tagtttcgct ccagcttaac ggccgtaacc cactccttaa
     9721 acttcgacaa taccagcttg gtagttctta ccagcgcatt ttttcgcccc gaggtgtcgc
     9781 gggggaacaa gtttttggca agctgctggc taatagtgct tccccctccg gcggtctttt
     9841 tcccaaatag aatggtttta accatcaccc ttcccaaacc ctttgcatct attccgctat
     9901 gggagtagaa acggttatcc tcggttgcaa tcagcgcatg gattacatta ggtgatattt
     9961 cgtcgtagcc gacaaacgtc ctgttttgaa cgtagaaatt acccagcacc acgttatcct
    10021 cggatatgat ttccgaggca atgttgcttt tcggattctc cagctcctcg aatgtgggca
    10081 tgggaccaaa aaattcgagg ttgatgagta tgaaaatcac caggaaaaaa agtagcagga
    10141 atgagataat tccccaaaac caccatttaa actttcggat tacctccgga tttttccaat
    10201 tcatgggctt aaaaatttag catcacgcgc tccctataaa cataaacttt aaccattcgc
    10261 cccttaggtc aactttcctt tgtgccagca agtgaaaacg tgcaacgttc acctggtagt
    10321 aaaacgaaca aagttaaaaa ctattttgga catagctcac aaccaggcaa ccggtggtaa
    10381 ggattgatag gctgatttcg aaaacaagag tggagcatgt tttcggtttt atggaacaaa
    10441 gtat
//
LOCUS       DAOSHM010000077        10285 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_5526_length_10285_cov_19.496774, whole genome shotgun
            sequence.
ACCESSION   DAOSHM010000077 DAOSHM010000000
VERSION     DAOSHM010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 10285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10285
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5526_length_10285_cov_19.496774"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            176..7210
                     /locus_tag="PK839_09435"
     CDS             176..7210
                     /locus_tag="PK839_09435"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOG72998.1"
                     /translation="MNNLKRIFLAGLTLVGSAASGQIYFTEGFENGTRPSGWSEIYVS
                     GSQVWLYRNGGHSPNDPSWSIPASQEDITRNPSSAHSGTYNAIFFKQSSAHEQTKLVT
                     KAIDLSEGIKPELSFWLCQVPWTFGGSTNNDELRVYYKNTFGGAWTLLAEYTDPIFDW
                     TEYKINLPNPSSTYYIAFEGKINWGFGTCIDDIVIEEKGYQERYVSELTVSQADFSFI
                     PSGSPNEPILKVGVKVFGNMGSATLNTLTAKSLNTSDNDIEANGVKLYFTPTGTFSAD
                     NLLVEGKNFTSGQVTFNNINFNLPNGQSYLWLTYDVKQNAVHGNIMDARLDAGSIQVS
                     DSLYPKVNESPEGNRVIYETIYRQDFEGTHQWLLSGEFQVDVPQGKGGAYIGFPDPTT
                     AYSGSKVLGTDLTGLGATQGDYENYVTEATTYKAISPIIDALFFKDLKISYRRWLNIE
                     VWDRASVEVSKDNGTSWSSIWHNNNYFSDNLWLKTAHDVPPTISRSNQLKFNFKLGPT
                     DGATTYSGWNVDDFIVTGDYITKDVGVVEWVYPLSGCGHSASDSVTVKIANLGALASP
                     TPIPVQYSFNNGTTWTTNYYTGSIPAGDTVTFTFSTRVNLSTPGIKYVKARTRLNGDE
                     DASNDELSAQVYIVPTYLLPYTENFESNDGYWRSYGPSLWQWGSISKPAMSGGSKSWA
                     TSLSQNYGTALTGAPDTLFEDNFEIPDGWTLTGEFERSTIIMGWDTIPTFAYSGIYCL
                     GTDLSRQGAHKGMYEPNSSYEALSPAVDISSYANLQLAYYRWFKVMEGDTAKVMASNN
                     GLTWTTIWNSAGQEIADENWTEEIIDIPASLVNGSSLTIKFVLKANGDENIAEGINID
                     DFRIIGNHFSTGMAYLQSPCFNFTGISIPVMDMSIFNRTEAGVDGTTLYYSTDKGETW
                     TPVDNNDTLDDYWNFYTDSLVSSIGLDGWNGLGSGWENTRHLLPAALANNSSVIFRLG
                     FKADKTNNDFGGTAIDNFKLLQAPFDVGVESILSPSTTCDLSQNQSIQVRLKNFGIQD
                     MEAGDTIIVGIDIDHPMVPDSFTDTLVLSSTLAVNSHLDYTISKTFNMSVSGDYQVTA
                     FTKIENNPVFYHPEPNDTASTLITVQKPYVELGNDIYSYNPENIVLDATNPDPTVTYK
                     WYKSPDLTTVIGTNPTYAIADHDGGKYLVTLTNSIPCSSTDSVTVNRIIRDVGISTLV
                     SPTSSCELSDETPLSVYVKNYGSDTLMSSDDEIYLHYTFNGNPQVDTTWTITQNLLPG
                     DSLLFTFNQTFDMQAVGSYPLHVWANTTLDEDAANDGPNTTIDVWGYPTFSLYQTMGL
                     PKDSVSVTLTSYTLDAGPTWDGYLWHNDSSTLQTYDMIETGWGVVTVQDNHGCPATDS
                     VYVDLQFSDVSVDEVVSPLTSCEIPGTTYPHIKIRNSGTNPISAGTDITLKYRLNGDL
                     KESSILTLPSSWNANEVMDVEFSVGVDISAPGTYSLDFIATTASDDKPENDSIRHEIT
                     VFGTPSLELGDSVFTRNTSYNLDAGSGHESYSWSTGETTQQISVTQTNTYWVEVVDDG
                     ICSVRDTVVVTFLRHDYSITEIVNPVTSCSTPIPQPVSVKLTNTGNDTLKSGQSIILG
                     YQIDDEYFAYENHTLLSNLNPGQFLLYTFADPVDLSAPANLQVLAYSYFPDDANASND
                     TLLVSIEIMESPTIDLGEDRVDRTGSITLDGGEGADYSYLWQDNSTNRFLIVENTGDY
                     WVTTTAPNGCFDQDTVYLTMLKPDYRVSAILSPSSACSLSNAESIQVEIENLGTDTIL
                     VGQNIFVSYEVNSILQQTQSITMTSKFKPGDKVYHTFSKTYNMANPGSYIIKAYTSFS
                     GDLNPFNNATTAPITNYGPPQVNLGDDLAFCDGSSAILDAGPGMASYLWNTGAISQTI
                     NVSTPGEYSVEVTDSHGCTNSDNIHITVNGLPTVTHLPLDPVCLDAEAFTLTGGNPAG
                     GSYTGSGVSGGTTFTPSGAGVGTHTLTYLYTDGNGCSNSTNVGITVNALPVVNLGSNQ
                     SIEEPLTLDAGAGFAAYQWQDGSTSQTYTVEATGLYSVTVTDGNGCQAYDEVYITFLE
                     TLDVIVSDLISPTDKCYDEVDEPVTVELTNRGTKTFTTGEQLNVSYQVGSAQPVTETL
                     TFSGSFAQNDKLQFTFSNNLALSTGNHTFKCYTSIAGEDGEIISYNVMVYNLPNLNLG
                     ADTIRHSLPYVLQAGVEAQTYLWSTGATGTASITVSQWGKYWLTITDSHGCVASDTTV
                     IWWPIGVEELPGTNDKVYIFPNPADKKLNIRIDGENEATFTIDLLNHQGIRVNSAKLQ
                     VPSELSTTFDVEGLAPGIYFLHVYSSKNSTVFKVVVKR"
     gene            7566..>10285
                     /locus_tag="PK839_09440"
     CDS             7566..>10285
                     /locus_tag="PK839_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005862535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOG72999.1"
                     /translation="MYKIQTHPILEVPKTESITFLFNGNEVKGAKGFTIAAALHQAGY
                     PVHSHSLENRERSLECGIGKCGACEMLVDGQIKRICITPVDGVKEVSEIPKDYAPGVK
                     PADKLHSTRVYKTTVAIIGAGPAGLAARELLLQHGIENIVIDNNATIGGQFNMQTHQF
                     FFFEKEKKFGGMRGFEIAKTLAGDNAEGIFLNSTVWDILENKRIAVKNIRTDEIYFVE
                     ADHLVVATGAVPFMPTFENDDLPGVYTAAVVQKMMNQEFTLLGKNVLTVGAGNIGYLT
                     SYQLMQAGAKVKAIIEAMPNEGGFPVQANRVRRLGIPIMLGKTLVKAVPNESHTGIVG
                     AIIADAVNFKPVPGTEQLLEGIDAINICTGLVSDDQLIIKGREVFGRNCHGAGDAIRI
                     GEGTSAVLRGKQVAYEILQELDVRFNYDNFLDISKEYIDSQQHPVRVIDKPFIPDEER
                     RRSKPFVQIDCLYGFACNPCTFACPQGAITKSSTSAVPQIDYTKCTGCFDCVYQCPGL
                     AIFGYSYTKDWLFLPIEYEAQEGAEVFLVDNNGKKLGEGIIEKILRKPNKTNVARVKS
                     LSLHGDDLLNARGFIVKSSYPQPLDTKPVSHKSEEKIYVCHCDDVTLDEVLETIGDRK
                     FISADEIKHTTRLGMGACRGKRCIKRLKTTLAPLGIQIVGDATPRGPLSNQLTLGELY
                     PKDVHETYITGVNGKTVRVEKVSALVAGGGIGGSALFRYMAEAGLKPILLNFGRGSSW
                     RNIAGGRTAFSLPEIADIAKVNHEIFRELHGISSIDYRPINYVNFAHDDSTLKALEVS
                     MAWSDAYMVEPKDFSKEISPYMNPSLATYKAAQITRGCWQATPGKTVDLLRKIGIRNG
                     GTVKEDCELISVYRDGAGYVALVRDHDHSYIEFHADSFVNALGPSGEKFNR"
BASE COUNT         2810 a         2664 c         2376 g         2435 t
ORIGIN      
        1 ttcttgaatc ctgcttgatt ttttttatcc tttatccttt accctttaac ctttttggat
       61 attttatcgt aaaataatat taatttgcat tagtaaacta cctcttaccc taacttaata
      121 atcatctatt aagctgataa atagatacgc taaaaccata agaaaatata ccctaatgaa
      181 taacctaaaa agaatatttc tggcgggctt aactcttgta ggttcggctg ccagcggaca
      241 gatatatttt accgagggat ttgaaaacgg taccaggcct tctggttgga gcgagattta
      301 cgtttcggga agtcaggtat ggctgtaccg caatgggggt cacagcccta acgatcccag
      361 ctggagcatt ccagcctccc aggaggacat cacccgcaac ccttcttcgg cccactcggg
      421 tacctacaac gccatattct tcaaacaaag ttctgctcac gagcagacca agctggttac
      481 taaggcaata gacctaagcg aggggataaa accagaactc agcttctggc tttgccaagt
      541 tccttggact tttgggggta gcaccaacaa cgatgagctg agggtgtact acaagaatac
      601 gtttggtgga gcctggacat tgctggccga atacaccgat cccattttcg attggacaga
      661 gtataagatt aaccttccaa acccctcgtc aacctactac atagcctttg agggtaaaat
      721 caactggggc tttggaacct gtattgacga cattgttatt gaggaaaaag gctaccagga
      781 aaggtatgtg agcgagctta ctgttagcca ggccgatttt tcattcattc caagcggttc
      841 ccccaacgag cccatcttaa aggttggggt taaggttttt ggtaatatgg gtagcgcaac
      901 actcaacacc ctaacggcaa agtcgctaaa caccagcgat aacgatattg aggccaatgg
      961 agtaaaactc tacttcaccc caaccggaac atttagcgct gacaacttgc tggttgaggg
     1021 gaaaaatttt acctcaggcc aggttacctt caacaatatc aacttcaacc tacccaacgg
     1081 gcagtcgtac ctatggctaa cctacgatgt gaagcagaat gctgttcatg ggaatataat
     1141 ggatgccagg ctggacgccg gaagcataca ggttagcgac tcgctttacc caaaggtgaa
     1201 cgaatcgccc gaaggtaacc gggtgatata cgaaacaatt taccgtcagg actttgaagg
     1261 gacacaccag tggttgctga gcggagagtt tcaggtggat gttccccaag gaaagggagg
     1321 agcatatatt ggttttccag acccaaccac agcctacagc ggaagcaagg tgttgggaac
     1381 ggaccttacc ggtcttggcg ctactcaagg cgactatgag aactacgtta ccgaggcaac
     1441 aacctacaag gctatttcac caataattga tgccctgttc ttcaaggatt taaagatctc
     1501 ataccgccgc tggctgaaca tagaggtttg ggatagggca tcggttgagg tgtcgaaaga
     1561 taatggaacc agctggagca gcatctggca taataacaac tacttttccg ataacctttg
     1621 gctcaaaact gcccacgatg tacccccgac catatccagg tcgaaccagc tgaagttcaa
     1681 ctttaagctt ggccccaccg acggtgcaac tacctactcg ggttggaatg tggatgattt
     1741 catcgttaca ggcgactata ttaccaagga tgttggggtg gtggagtggg tttaccccct
     1801 ttcgggctgc ggtcattccg cctcggacag cgttacagtt aaaattgcca atcttggcgc
     1861 tttagcttcg ccaacaccaa tacccgtgca gtactccttc aacaacggta ctacatggac
     1921 aaccaactac tacaccggta gcattccggc gggcgatacc gttactttta ccttcagcac
     1981 ccgggttaat ctttctaccc ccgggattaa atacgtaaaa gcccggacca ggcttaacgg
     2041 cgatgaggat gcctccaacg atgaactttc tgctcaggtt tacattgtac ccacctatct
     2101 cctaccttac accgaaaact ttgagagcaa cgacggctac tggcgttcgt atggaccctc
     2161 cttatggcag tggggcagca tttccaaacc ggctatgagc ggcggttcaa aatcgtgggc
     2221 aaccagcctt tctcaaaact acgggacagc gctcaccggt gcacccgaca cgctatttga
     2281 ggacaacttc gaaatccccg acggctggac gctgacgggc gagttcgaac gcagcaccat
     2341 aatcatgggt tgggacacca ttccaacctt tgcttatagc ggcatatact gcctcggaac
     2401 cgaccttagc agacagggtg cccacaaggg catgtatgaa cccaattcca gctacgaggc
     2461 attgagcccg gctgttgaca tcagcagcta cgccaacctg cagctggcct actatcgttg
     2521 gtttaaagta atggagggcg atacggccaa agttatggcc agcaacaacg gccttacctg
     2581 gaccaccatc tggaacagcg ccggacagga aatagccgac gaaaattgga ccgaagaaat
     2641 tattgatata cccgcctcgc ttgttaatgg cagcagcctt accatcaaat tcgtcctcaa
     2701 agcaaatggc gatgagaata tagcggaggg gataaatatt gatgacttta gaattatcgg
     2761 caaccatttt tcaaccggaa tggcctacct gcaaagccca tgcttcaact ttaccggtat
     2821 ctccataccc gtgatggaca tgagtatttt taaccgcact gaagccggag ttgacggcac
     2881 cacactttac tactccaccg ataagggcga aacgtggact cctgttgaca acaacgatac
     2941 cctggatgat tactggaatt tctacaccga tagcctggtt tcatccatcg gccttgatgg
     3001 atggaacggg ttgggaagtg gttgggagaa cacccgccac ctgcttcctg ctgctctggc
     3061 taataatagt agcgtaattt tcaggttagg cttcaaagcc gataaaacca ataacgattt
     3121 tggaggaact gccattgata actttaaact actccaggca ccatttgatg tgggtgtaga
     3181 aagcatatta agcccttcca ccacctgcga tttaagccag aaccaatcca ttcaggttag
     3241 gttgaaaaac ttcggcattc aggacatgga ggctggcgat accataatcg tgggcattga
     3301 tattgaccat cccatggttc ccgactcctt caccgacacc ctggtgctga gctccacgct
     3361 ggctgtgaac agccacctgg actacaccat ttccaaaacc ttcaacatgt cggttagcgg
     3421 cgattaccag gttacagctt ttaccaagat tgaaaacaac ccagtattct accacccgga
     3481 gcccaacgat actgcttcta cattaattac cgtgcagaaa ccctatgttg agctgggcaa
     3541 tgatatttac tcctacaacc ccgaaaacat tgtgctggat gccaccaacc ccgaccccac
     3601 cgtaacctat aaatggtaca aatcgccgga cctgactaca gttattggta ccaatccaac
     3661 ctacgccatc gccgaccatg atggaggaaa ataccttgtt acgcttacaa attctattcc
     3721 ctgctcctca accgactcgg ttaccgttaa ccgtatcata cgcgatgtgg ggatttcaac
     3781 cctggttagc ccaactagct cttgtgagct ttccgatgaa acgcccctga gcgtttacgt
     3841 aaagaattac ggatccgata cgctcatgag tagcgacgac gaaatatacc ttcattacac
     3901 ttttaacggc aacccccagg tggataccac ctggaccatt acccaaaacc tgcttcccgg
     3961 cgattcgcta ctattcacct tcaaccaaac tttcgatatg caggctgttg gttcttaccc
     4021 gttacatgtt tgggcaaaca ccacactaga tgaggatgcc gctaacgatg ggcctaacac
     4081 caccatagac gtatggggct acccaacctt tagcctttac cagaccatgg gtctgccaaa
     4141 agattcggtc agcgtaaccc taacctccta cacccttgac gccggtccaa catgggatgg
     4201 ctacctgtgg cataacgaca gctcaaccct ccaaacctac gacatgatcg aaacgggatg
     4261 gggtgtagtg acggtgcagg acaaccacgg ctgccccgct accgattccg tttacgttga
     4321 cctgcaattc tccgatgttt cagtggatga ggtggtttca ccattaacct cctgcgaaat
     4381 tcccggtacc acctatcccc atataaaaat aaggaattcc ggcaccaacc cgataagcgc
     4441 aggaaccgac attacgctga agtacaggct caatggcgat ttgaaggaaa gcagcatttt
     4501 aacacttccc tcaagttgga atgcaaacga ggtaatggat gtggagttct cggttggcgt
     4561 tgacatatcg gctccgggaa cctactccct cgactttata gcaaccaccg cctccgacga
     4621 taaaccagaa aacgacagca taaggcatga gataacggtg ttcggaaccc catcactgga
     4681 gctaggcgat tctgttttta cccgaaacac cagctacaac cttgatgccg ggtcgggcca
     4741 tgaaagctat agctggtcta ccggcgaaac cacccagcaa atatccgtta cacagaccaa
     4801 cacctactgg gtagaggtgg ttgatgacgg aatatgctct gtaagagaca ccgtggtggt
     4861 taccttcctc cggcacgatt actccataac cgaaatagtt aaccccgtta catcgtgcag
     4921 tacgcctatt cctcaacctg tgtcggttaa gcttaccaac accggtaacg atacccttaa
     4981 atccggacag agcataattc ttggttatca gattgacgac gaatactttg catatgaaaa
     5041 ccatacactc ttgagcaacc ttaacccagg gcagttcctg ctctacacct ttgccgaccc
     5101 cgttgacctg tcggctcctg caaacctgca ggtgcttgcc tacagctact tccccgatga
     5161 cgccaatgcc tccaacgata cccttttggt ttctatcgag atcatggaat cgcctacaat
     5221 tgatttgggc gaagacaggg ttgaccgaac agggagcatt acgctcgacg gaggtgaggg
     5281 cgctgattac tcctacctgt ggcaggataa ctctaccaat aggttcctta ttgttgaaaa
     5341 taccggtgat tactgggtta ctaccactgc gcccaacggc tgcttcgatc aggataccgt
     5401 ttacctcacc atgctgaaac ccgactacag ggtttcggct atactttctc cctcgtccgc
     5461 ctgctccctg tccaacgctg aaagcattca ggttgaaatt gaaaatcttg gtacagacac
     5521 gatacttgtt gggcaaaaca tatttgtttc ctacgaggtg aatagcatcc tgcagcaaac
     5581 ccagagcata accatgactt ctaagtttaa gcccggcgac aaagtttacc acaccttttc
     5641 caagacttac aacatggcaa atccaggaag ttacatcatt aaggcctaca cctcattttc
     5701 gggcgacctt aaccccttta acaacgccac aactgcacct ataaccaact acgggccccc
     5761 acaggttaat ctgggagatg atttggcatt ttgcgatggc tcctcagcca tccttgatgc
     5821 cggacctggc atggccagct acctgtggaa taccggcgca atcagccaaa caatcaacgt
     5881 atctacacca ggtgagtact ccgttgaggt taccgatagc catgggtgta ccaattccga
     5941 taacattcac attacggtta acgggctacc aacggttacc cacctacctc ttgatccggt
     6001 ttgtcttgat gcagaagcct ttacccttac agggggtaac ccagccggag gcagctatac
     6061 gggtagcggg gttagcggcg gcacaacttt taccccatcc ggtgccggag tagggacaca
     6121 taccctgacc tacctctaca cggatggtaa tggttgctcc aactccacca acgtgggtat
     6181 cacggtgaat gcgctgcctg tggttaattt gggttcaaac cagtctattg aggaaccgct
     6241 taccctcgat gccggggcag gctttgctgc ctaccagtgg caggatgggt caaccagcca
     6301 aacctatacc gtggaggcaa ccgggctgta ctcggtaacc gttaccgatg gcaatggctg
     6361 ccaggcctac gacgaggtct atatcacctt cctagaaacc ctggacgtga ttgtttccga
     6421 cctaatctcc cccaccgata agtgctacga tgaggttgac gagccggtta ccgtggagct
     6481 caccaacagg ggaaccaaaa cctttaccac cggtgaacag ctaaatgtta gctaccaagt
     6541 tggctctgca cagcctgtta cagaaacact tacattctcg ggtagctttg cacagaacga
     6601 caagctgcag tttacctttt caaacaacct tgcgctaagt accggtaacc atacctttaa
     6661 atgctacacc tccattgccg gggaggatgg tgaaataata agctacaatg taatggttta
     6721 caacctaccc aacctcaacc ttggtgcaga taccatcagg cattctctcc catacgtgct
     6781 gcaagcaggg gttgaagcgc aaacctacct ctggagtacg ggagcaaccg gtaccgcctc
     6841 tattaccgtt tcgcaatggg gaaaatactg gctaacaata acagatagcc atggttgcgt
     6901 tgcatccgac accacagtaa tatggtggcc tattggcgtt gaggaactcc ctggaaccaa
     6961 tgataaggtt tacatatttc caaacccagc agacaaaaaa ctaaacatta gaattgacgg
     7021 ggagaatgaa gctaccttta ccattgacct cctaaatcat cagggcatca gggtcaactc
     7081 ggccaaattg caagtacctt cggagcttag cactaccttc gacgttgagg gattggctcc
     7141 ggggatttac ttcctgcatg tttactcatc taagaattcc actgtgttta aggttgttgt
     7201 gaaaagataa ccaacaaatt cggtaggcaa ggtgctaaac gctcaaccca gaatttcact
     7261 gaaatggggt tgggcgtttg gcagtattat ctggtcaata aacaggtcta ctgaaaatgg
     7321 gtattcccaa aagcgagcct acaacccttt ctttccatca ccttctacat tgaaataaaa
     7381 catgcaacca cttaatttta agattgaggt caatgagtaa acaacccctc tcataagttt
     7441 taccccacga attcacatat tttttataaa ccctgacgaa tatcattaac agccatactt
     7501 ggtaattcat acttttgtaa tcaaattaca agaaattcta tcatagttct tctaaatggc
     7561 aagttatgta taaaattcaa acacacccca tacttgaggt tccaaaaacg gaaagcatta
     7621 cttttctatt taacggtaat gaagtaaagg gggcaaaagg atttaccatt gcagcggcgc
     7681 ttcatcaggc aggatatccg gttcatagcc acagtctcga aaatcgggaa cgtagcctcg
     7741 aatgcggtat aggcaagtgt ggagcatgcg agatgctggt agatggtcaa attaaacgta
     7801 tttgcattac ccctgttgat ggggttaagg aggttagtga aatacccaag gactatgcac
     7861 cgggcgtaaa acctgctgat aagctgcaca gcaccagggt ttataaaacc accgtggcca
     7921 tcattggcgc cgggcctgcc ggcttggcgg cacgcgagct gctgcttcaa catggtattg
     7981 agaacattgt gattgataac aacgcaacaa ttggcggaca gttcaacatg caaacccacc
     8041 agttcttctt ctttgaaaag gagaagaagt ttggcggaat gcgaggcttc gagattgcca
     8101 aaaccctggc cggcgataac gccgagggta tttttctgaa cagcaccgta tgggacattc
     8161 tcgagaacaa gcgcattgcg gttaaaaata tccgaacgga tgaaatctac tttgttgagg
     8221 ccgaccacct ggttgtagcc acaggagcag ttccgtttat gcccactttt gagaatgacg
     8281 atttacccgg tgtttacacg gcagcggttg ttcagaaaat gatgaaccag gagtttaccc
     8341 tgttgggaaa aaatgtgctt acggttgggg ccggcaacat tggctacctt accagctacc
     8401 agctgatgca agctggtgct aaggtaaaag ccattattga ggccatgccc aacgaggggg
     8461 gatttccggt tcaggccaac cgtgtccgcc gtctcggaat acccattatg cttggaaaaa
     8521 cactggtgaa agcagtgccc aacgagagcc acacgggaat tgttggagcc attattgccg
     8581 atgctgtaaa tttcaagcct gttccaggca ccgagcagct gctcgaggga attgatgcca
     8641 ttaacatttg tacaggtctg gtttccgacg accagcttat catcaagggc agggaggtgt
     8701 tcggacgtaa ctgccatggc gccggcgatg ccattcgcat tggcgaggga acaagcgcag
     8761 ttcttagagg caagcaggta gcctacgaaa tactccagga gctcgatgta aggttcaact
     8821 acgacaactt cctcgacatc agcaaggagt atattgatag ccagcaacac cctgtaaggg
     8881 taattgataa acctttcatc cctgatgagg agcggagaag gagcaaaccc tttgttcaga
     8941 tagactgcct ctatgggttt gcatgcaacc catgcacatt tgcctgccca cagggagcca
     9001 taacgaaaag ttcaaccagc gctgttcccc aaattgacta caccaagtgc accggctgct
     9061 tcgattgcgt ttaccagtgc cccggcctgg caatattcgg ttacagctac accaaggatt
     9121 ggctgttcct gcccatagag tacgaggccc aggaaggcgc tgaggttttc ctggttgata
     9181 acaacggcaa gaaacttggc gagggtatca ttgaaaagat tctccgaaaa cccaacaaaa
     9241 ctaacgtggc cagggtgaaa tcgctttcac ttcacggtga cgacctactc aacgctcggg
     9301 gatttatagt gaagagcagc tatccccaac ccctcgatac caaaccggtt tcgcataagt
     9361 cagaggaaaa aatctacgtt tgccactgcg atgatgttac gcttgacgag gtgctggaaa
     9421 ctatcggcga ccgaaagttt atttcagccg atgaaattaa gcataccacc cgcctgggca
     9481 tgggcgcctg ccggggaaaa aggtgtatta aaaggctgaa gacaaccctg gctccactgg
     9541 gcattcagat tgtgggcgat gccacacccc gtgggccgct tagcaaccag ctcacacttg
     9601 gagagctcta cccaaaggac gttcacgaaa cctacatcac cggagtaaac ggaaaaacag
     9661 ttagggttga aaaggtttct gccctggttg ccggcggagg aataggagga agcgccttgt
     9721 tccgctacat ggccgaggcc gggttaaaac ccatcctgct taattttggt agaggctcca
     9781 gctggcgaaa cattgccggt ggaagaacag cattctccct tcctgaaatt gccgacattg
     9841 ccaaggtaaa ccatgaaata ttcagggagc tgcatggcat ttccagtatt gactaccgcc
     9901 ctataaacta cgtgaacttt gcccacgacg atagcacgct aaaagcactg gaggtctcca
     9961 tggcctggag cgatgcctac atggttgaac caaaggattt cagcaaggaa atttcaccct
    10021 acatgaaccc ttcgcttgcc acctacaaag ccgcacagat tacccgtggc tgctggcaag
    10081 ctacccctgg caaaacggtt gacctactcc gtaagattgg aatcaggaat ggaggaacgg
    10141 ttaaggagga ctgcgagctg attagcgttt accgcgatgg agcaggatac gtagctcttg
    10201 tgcgtgacca cgaccacagc tacattgagt tccatgccga tagctttgtg aatgcacttg
    10261 gcccgtcggg tgaaaaattc aaccg
//
LOCUS       DAOSHM010000078         9699 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_5974_length_9699_cov_19.829739, whole genome shotgun sequence.
ACCESSION   DAOSHM010000078 DAOSHM010000000
VERSION     DAOSHM010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 9699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9699
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5974_length_9699_cov_19.829739"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(49..122)
                     /locus_tag="PK839_09445"
     tRNA            complement(49..122)
                     /locus_tag="PK839_09445"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(86..88),aa:Met,seq:cat)
     gene            418..828
                     /locus_tag="PK839_09450"
     CDS             418..828
                     /locus_tag="PK839_09450"
                     /inference="COORDINATES: protein motif:HMM:NF039344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="START-like domain-containing protein"
                     /protein_id="HOG73000.1"
                     /translation="MKNDEGNGVYEVKLEYRVNTTPNILFQRLFTPYGLSEWFADDVN
                     LLGNTYTFIWNGSTRKAELVEKKENKYIKFRWIEAEGNSKTNYHFTFQLNRDDITNEL
                     SLLVTEEIEDLEERDEIISLWNYQINELKKAIGA"
     gene            915..2372
                     /locus_tag="PK839_09455"
     CDS             915..2372
                     /locus_tag="PK839_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOG73001.1"
                     /translation="MKTLHRFVLKSYLGPLVLTFFIVMFTLLMQFLWKYIDDLVGKGL
                     DGSVITELLLYASAGLVPMALPLATLLAALMAMGNLGENNELLAMKSAGISLPRILEP
                     LVVVIILISVGAFFFSNNVLPYTNLKMSTLLYSVKQQKPELIIKEGVFTNLIEGYSIK
                     VAEKDTKTNLMHRILIYDHSSDEGNNTVTYADSGYMKPTGDQKFLIATLYNGYSYKEV
                     KEKSGSRRNPSTNYPAQKQYFEKQVIVFELKGFDLQRTDESFFKDSYQVLNLSQLEKA
                     EDSLSTALNLQLESFSQNLTQNSIMRNPNWLKRTGSSPVSYKLDFDSLFSKLSPEQKQ
                     MAIARALDNARSTKIFVSNSKDEFKYRQKYISRHKIEWNRKFTLSFACFVFFFIGAPL
                     GAIIRKGGLGMPVVISVLFFIAYYIITITGEKFARELVWSPGLGMWVSSFILLPLGIF
                     LSYKATNDSSLMNADFYTEKLKKILRLGRKAKSNK"
     gene            2420..3625
                     /locus_tag="PK839_09460"
     CDS             2420..3625
                     /locus_tag="PK839_09460"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOG73002.1"
                     /translation="MRILVLAHKPPFPPVDGGTLATLNMCIGLVKAGNTVTVLTLSTP
                     KHPSSIERIPESLLSEINFEILSIPLTTTPFGYFYNILFTRRPYNIQRYFSKTFKRII
                     LKTLRSNEFDIVQLEGLYLYPYVKTIRKHFSGKIVLRAHNVEHHIWETLANEESNKLR
                     AWYFNLLARRIARIERHINSNIDALVAISDPDYKWFTTNGLSKPAVVCPAGYFPPQQP
                     LESTPPDKPSLCYIGALDWMPNIEALMWFIDWVWPRIQSEIPEVEFYVAGRNATSELA
                     ERLMMERNIVFYGQVADSATFLSAYPVMIAPLFSGSGIRVKIIEGMFLRRAIVATSMA
                     SRGIKAVNNEHLLIADAPDEFAQAVCRLLSSPETASYISKNAQEFAFSHFDATHLAKE
                     LTNFYQRLI"
     gene            3625..4812
                     /locus_tag="PK839_09465"
     CDS             3625..4812
                     /locus_tag="PK839_09465"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOG73003.1"
                     /translation="MATSIWIFWLLAFILLYTYAGYTFLLWITAKIYSWTRSTATNME
                     SSAEELPAVTMVVAAYNEKAVVSQKIANINQLNYPPEKVFQLWVTDGSTDGTEEVLKD
                     YPEVSIIHHTERLGKAEAINRAMKLVQTPVTVFSDANTIVEPNSLIELVKPLTDSKTG
                     GVAGEKRILNSKNGNPTSAGEGIYWRYESFIKSMESATGSTISAAGELFAIKTELFTP
                     IPPDTLLDDFEISSRIILGGNRVKYSRYAAASEYGSLNFSEEMKRKVRIAAGGFQFLF
                     RNLQLLNPFPHPGYTFKYFSHKVLRWAFAPACLFLLPFFNAIIITAESENPFYLLTLV
                     ILLSFFLLGLFGHLARNRKAIPHLMFLPYYAILMNISEIVGFFKFIQHKHNPKWDKSK
                     RET"
     gene            4885..6123
                     /locus_tag="PK839_09470"
     CDS             4885..6123
                     /locus_tag="PK839_09470"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019129438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HOG73004.1"
                     /translation="MIKEDNSSIKLNTIEEALEEIRKGKVIIVVDDEDRENEGDFIAA
                     AELITPEIINFMAKHGRGLICAPLPEKRCDELELDLMVDRNTAPHNTAFTVSVDLLGH
                     GCTTGISASDRAKTVKALVNPSTTPEDLGRPGHIFPLRAKDKGVLRRPGHTEAAVDLT
                     RLAGLTPGGVLVEIMNDDGTMARLPQLLEIAKKFDLKIISIKDLIAFLLHSETIIERG
                     FKVKLPTEFGYFDLIPFRQKSNGLEHVALIKGEWEADEPILVRVHSSCVTGDIFGSYR
                     CDCGPQLHRAMQMIEHEGKGVLVYLNQEGRGIGLFNKIHAYKLQEEGLDTVEANIELG
                     FQPDERDYGVGASILHEVGVSNMKLITNNPKKMRGLEGYGLKIVENIPLVIPSNPFNE
                     VYLKTKVEKMGHINLFGPKQ"
     gene            complement(6173..6751)
                     /locus_tag="PK839_09475"
     CDS             complement(6173..6751)
                     /locus_tag="PK839_09475"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HOG73005.1"
                     /translation="MPGVEAQLLMSPSIRKNVNGNSSSNGQTRQSAVLILFFPDEDET
                     RLVLIKRQTYEGLHSGQVSLPGGKWEEVDGSLEQTALREAFEEVGVNPERVELVGRLT
                     PLFIPVSNMMVQPVVGFTPEKPDFHPNLQEVDYIITVPVEQLFDQKNKSVRVISVNGS
                     PITAPYYNVNGEFVWGATAMILAELEAVASRK"
     gene            6975..8354
                     /locus_tag="PK839_09480"
     CDS             6975..8354
                     /locus_tag="PK839_09480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG73006.1"
                     /translation="MKKIILISTLALLVASTGCVSKKYLKRGIKYEEAGLWELAAESY
                     IQSLTSKRDNLDAIVGLKRTGQKVIDEKCYKSIAAYESDNLKEAVYDYLDAVDFSKRT
                     SSLGVELMVNSRATDYFKDAKPRYIETLYAQAQQQMDAEKFQQAEALLDEIKKLEPDY
                     GNVKEMLKVSKCEPLYRQGKQLMESGYYRKSYGNFNQIVTEFGSYKDAAMLMGDALQK
                     AIITIKVNNFTGGYCTKALTQRIQSSVVASVNTLNNPFVKIVDSDNTIQIIEEQQRSL
                     TQGSDIEVGKILAAKAILTGEVLECKQDAGRLNKSDKRGYLKEVTKVKNPVTGETENK
                     VEYKKVSYEVYSIQNRVTFSLKFQLTSTETGAVMVSDIARSQQADETTYAVFNGKPEK
                     LVPGYWENINKDSPKDFVSDNPTDVSALQKMLQARREISSVDQLTDKAIAALSKQVSE
                     RINRYNPEQ"
     gene            8360..>9699
                     /locus_tag="PK839_09485"
     CDS             8360..>9699
                     /locus_tag="PK839_09485"
                     /inference="COORDINATES: protein motif:HMM:NF014248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPP20 family lipoprotein"
                     /protein_id="HOG73007.1"
                     /translation="MVKNSYSTIALASIILLLAACSGTKKSVDTIPSPSWVQNRPTSS
                     IYYVGIGSAPKIGSPNDYQQAAKQNALADMASEISVKISSNSVISAFETQQNFYEDYS
                     ASIRAEAQKELEGYEMAEAWEDSQNYWVYYRLSKETYRKISDEKKAKAASLSLDYYDK
                     ALKSAENGDYRTCLLQVVRALETLKPYFAESVTASYQGREIFLGNELIQLLTETLNNL
                     LLNGPSEVMARMGQPIPAGSLTYSVTNRIGIPQKGMPLRASYSEKPMLMNKSITGSDG
                     KACFSVDRVSSSRNVEKIAVVLDFSAIVQEATTDFDLRKLLSRFQVPSVETSIRIDKP
                     TFSVVTREMLFDKPAQKNVLAEAIKRKVTDEGFPIAEKTSDADFAITIEASTAKTGES
                     GSYKQASLSALIYVTNAQGVEVYRRSLDPITGSHFDWEAAGLSTFDKAAKRLESS"
BASE COUNT         2857 a         2338 c         2107 g         2397 t
ORIGIN      
        1 aaaaaaacac ttacatcgtt ttatgatata agtgcttgat ttttagagta gcgagggcgg
       61 gaattgaacc cgcgacctca tgattatgaa tcatgcgctc taaccagctg agctacctcg
      121 ccctaaagca tttatcgctg attattctcc cttaatctca gcacacccat gcctggttcc
      181 aggaacgtat taactagtca tgaaaaaaca tgccaagacc cacatttgtg ccgcaaaagt
      241 agaaaataat tcaatacctc aaaaaaatgt tcggtaaata tttagcatca acccgcaact
      301 ttgcggttta gaaaatggtt atcaacacga attgttaata aataatatcc aggagcttag
      361 gcaaaatctt cctaaaaagt tatattgcaa gtctagttga agtaaacaat ggagaacatg
      421 aaaaacgatg agggaaatgg ggtatacgag gtgaagctgg agtacagggt taacaccacc
      481 ccaaacattc tattccaaag gctttttacc ccctacgggc tgtcggagtg gtttgccgat
      541 gatgttaacc tgcttggcaa cacctacacg ttcatctgga atggttcaac ccgaaaggcc
      601 gagttagtag aaaaaaagga aaacaagtac attaagtttc gttggattga ggccgagggg
      661 aactccaaaa caaactacca cttcaccttc cagctgaaca gggatgatat taccaacgag
      721 ctttcgctgt tggttacaga agaaattgaa gatttggagg aaagggatga aattatctcg
      781 ctttggaact accaaattaa cgagctaaaa aaggccattg gcgcataatg atagagttga
      841 attgcctccg gtagagctat ttctgggaaa atggttacct ttgcccttgt ttttaaccat
      901 atgagggcaa attcgtgaaa acactgcatc gttttgtttt gaaatcatac cttgggccac
      961 ttgtgctcac cttctttatt gtcatgttta ccctgctcat gcagtttctt tggaaataca
     1021 ttgacgattt ggtaggtaaa ggccttgatg gaagtgtgat taccgaacta ttgctctatg
     1081 cttcagcagg tttggtaccc atggcgctgc ccttggcaac gcttttagca gcattaatgg
     1141 ccatgggcaa cctgggcgaa aacaacgagc tgctggccat gaaatcggcg gggatatcac
     1201 tccctcgaat tttggaaccg ctagtagttg tcattatcct catcagcgta ggcgcttttt
     1261 tcttttctaa caacgtgctt ccctatacaa accttaaaat gtcaaccctt ctatactccg
     1321 ttaagcagca aaaaccggag ttgattataa aggaaggcgt tttcacaaac cttattgagg
     1381 gttacagcat caaggtggct gaaaaggaca ccaaaactaa ccttatgcac cgcatcctca
     1441 tatacgacca cagctccgac gagggcaaca acacggttac ctatgccgac tcaggctaca
     1501 tgaagcccac aggcgatcag aaatttttaa ttgccaccct ttacaacggg tattcctaca
     1561 aagaggttaa ggaaaaatcg ggctcacgaa gaaatcccag cactaactac cctgcccaaa
     1621 aacaatattt tgaaaaacag gtgatagttt ttgagctcaa gggatttgac cttcagcgca
     1681 ccgatgagag ctttttcaag gacagctacc aggtgctaaa cctttcacaa ctcgaaaagg
     1741 cagaggattc gctctccacc gccctaaacc tgcagcttga aagtttttcc cagaacctta
     1801 cccaaaacag catcatgcga aatcctaact ggcttaagag aaccggttct tcgccggtat
     1861 cctacaagct ggattttgat tcgctgttca gcaagctatc accggaacag aaacaaatgg
     1921 ccattgccag agctctcgac aatgcccgct ccaccaaaat attcgtatcc aactcaaagg
     1981 atgaattcaa gtacaggcaa aagtacatca gccggcacaa gattgaatgg aacaggaagt
     2041 tcacgctttc ttttgcctgc ttcgtattct ttttcattgg agctcccctg ggggcgataa
     2101 tcagaaaggg tggattgggt atgccggtgg taatttcggt tcttttcttt atcgcatact
     2161 acataataac cattaccgga gaaaagtttg cccgagagct ggtctggagt cccggcctgg
     2221 gcatgtgggt ttcatcattc atactgctac ccttgggaat ctttctatcc tacaaggcta
     2281 caaacgactc ttcgctaatg aatgccgatt tctacaccga aaaactcaaa aagatacttc
     2341 gacttggccg gaaggcaaaa agcaacaagt aaccacaggc aattaacatc tccctcatag
     2401 gagctatatt catacggcaa tgagaatact ggttctagcg cataaaccgc cattccctcc
     2461 agtggacggt ggaactctgg ccacccttaa catgtgcata ggacttgtga aggcaggaaa
     2521 cacggtaacc gtactcacgc tcagcacccc caagcacccc agctctattg agcgaatccc
     2581 ggaatctctt ctctccgaga ttaacttcga aatcctgagc attccgctga ccacaacccc
     2641 ttttgggtat ttttacaaca tcctgttcac ccgaagacca tacaatatcc aacgatactt
     2701 cagcaaaacg tttaaacgaa taattcttaa aacccttcgc agcaacgagt ttgatatagt
     2761 tcagctggag ggactctacc tctaccccta cgttaaaacc atccgaaagc actttagcgg
     2821 taaaattgtt ttgcgggccc acaatgtaga gcatcatatt tgggaaactt tggctaacga
     2881 ggaaagcaac aagctaaggg catggtactt caacctcctg gctcgacgta tagcccggat
     2941 tgaaagacat ataaacagca acattgatgc cctggttgcc attagcgatc ccgactacaa
     3001 gtggtttact actaatggct taagcaaacc cgctgtggtt tgtcctgccg ggtattttcc
     3061 gccacaacag ccactggagt caacccctcc cgataaacca tcactctgct acattggagc
     3121 gcttgactgg atgcccaaca tcgaagccct gatgtggttc atagactggg tttggccaag
     3181 gatacaatcc gaaattcccg aagttgaatt ttacgttgcc gggcgaaacg ccaccagcga
     3241 gttggccgag cggcttatga tggaaagaaa tattgtcttt tacggacagg ttgccgattc
     3301 ggccacattc ctcagcgcct atcccgttat gatagccccg ttgttctcgg gaagtggaat
     3361 acgggtgaaa attatcgagg gcatgttcct ccgaagagca attgtggcaa caagcatggc
     3421 ttcaaggggt ataaaggctg ttaacaacga acacctactc atagccgatg cccccgacga
     3481 atttgcccaa gctgtctgca ggctattaag ctcacccgaa actgcaagct atatctctaa
     3541 aaatgcacag gaatttgcct tcagccattt tgatgcaacc cacctggcca aggagcttac
     3601 taatttttac caacgcttga tttgatggct acgagcatct ggatattttg gcttctcgcc
     3661 ttcatcctac tttacaccta tgccgggtac acctttctac tctggattac cgcaaagata
     3721 tactcatgga ctcgttccac cgcaaccaac atggagagtt cagccgaaga gctgcctgcg
     3781 gtaaccatgg ttgtggctgc ctacaacgag aaagctgtgg tgtcgcagaa aattgccaac
     3841 atcaaccagc taaactaccc accggaaaaa gtcttccagc tatgggtaac cgatggttca
     3901 actgacggga cagaagaggt gctaaaagat tacccggaag ttagcatcat tcaccatacc
     3961 gaaaggttgg gtaaggctga ggctatcaac agggctatga agctggtgca aaccccggtt
     4021 accgtttttt cagacgccaa taccattgtt gaacccaact cgcttattga acttgtaaaa
     4081 ccgctcaccg actcaaaaac gggtggagtt gccggtgaga aaagaatact aaacagtaag
     4141 aatggtaatc caacctcagc cggtgaaggg atttactgga ggtacgaatc atttattaaa
     4201 agcatggagt cagcaaccgg atcaaccata agcgcggcag gtgagctctt tgccataaaa
     4261 accgaacttt ttaccccaat accccccgac acgctgcttg acgattttga gatttcttca
     4321 cgtattatcc tgggtggtaa ccgggtaaag tactcaagat atgcagctgc cagcgagtat
     4381 ggttcgctca actttagcga ggagatgaag cgtaaagtca gaattgctgc ggggggattt
     4441 caattccttt ttcgcaacct gcagcttctt aacccattcc cccaccccgg ttataccttt
     4501 aagtattttt cccataaggt gcttcgctgg gcgtttgctc cagcttgtct ttttctcctt
     4561 ccttttttca acgccatcat cattacagcc gaatcagaaa atccgtttta cctgctcacc
     4621 ctagtaatcc tgctctcctt ttttctgctg ggtctatttg gccatttggc acgaaacaga
     4681 aaggcaatac cccacctcat gttcctaccc tactatgcaa tcctaatgaa catttcagaa
     4741 attgttgggt tctttaagtt tattcaacat aagcacaacc ctaaatggga caaatcgaaa
     4801 agagaaacat gacaattgtc cggaatattt attgctccaa aaagctactt ttgcaaacga
     4861 gtttcgaaaa atacacatca agaaatgatc aaagaggaca atagctccat aaagctcaac
     4921 accattgaag aagcgctgga ggaaattcgt aagggtaaag ttatcatagt ggtggatgac
     4981 gaagacaggg agaacgaagg cgatttcatt gcggcagcag aacttattac tcccgaaatt
     5041 atcaacttca tggccaagca cgggaggggt ttaatttgtg ctcctctacc tgagaaacga
     5101 tgcgatgagc tggaactcga ccttatggtt gataggaaca ctgcccccca caacaccgct
     5161 ttcaccgttt ctgttgactt gcttggacat ggttgcacca cagggatatc ggcatccgat
     5221 agggcaaaaa ccgtcaaggc tcttgttaac cccagcacaa cccctgaaga tttgggacgc
     5281 ccaggccaca tatttcccct cagggctaag gacaaaggcg tacttagaag gccaggacat
     5341 accgaggcgg ccgttgacct cacccgtttg gcagggttaa caccgggagg agtgctggtg
     5401 gagattatga atgacgatgg aactatggcc agactcccgc agctgctcga gattgcaaaa
     5461 aaattcgacc taaaaataat ttccataaaa gaccttattg ccttcctgct ccattccgaa
     5521 accatcatcg agagaggttt caaggttaaa cttcctaccg agttcggcta cttcgacctt
     5581 attcccttcc gccagaaatc aaatggactt gaacatgttg ccctaatcaa gggggaatgg
     5641 gaggccgacg agcctatact ggtaagagtc cactcctcct gtgttactgg tgatatattt
     5701 ggctcctaca gatgcgactg tggaccacag ctacaccgcg ccatgcagat gattgaacat
     5761 gagggaaaag gagttttggt ttacctaaac caggagggca gaggaattgg cctgttcaac
     5821 aaaattcacg cctacaagct acaggaagaa gggctggaca ccgttgaagc aaatatcgag
     5881 ctgggctttc aacccgatga acgcgattat ggcgtaggag ccagtatact ccacgaggtt
     5941 ggtgttagca acatgaagct cattaccaat aatccgaaaa agatgagggg tctcgagggt
     6001 tacggtttaa aaattgttga aaatattccc ctggttatcc catcaaaccc cttcaacgag
     6061 gtgtacttaa aaaccaaggt ggagaaaatg ggccatatca acctttttgg acctaagcag
     6121 tagcctctcc aaattaatct accactacct ccaagccaaa agccccatag acctactttc
     6181 gggaagcaac cgcttcaagt tctgccagaa tcatggctgt tgcaccccac acaaactcgc
     6241 cattcacatt atagtaggga gctgttatgg gtgaaccatt aaccgatata accctcaccg
     6301 atttattttt ttggtcaaaa agctgctcca ccggtacggt tatgatgtag tcaacttcct
     6361 gtaggttggg atgaaaatcg ggtttctcgg gcgtaaaccc caccactggc tgcaccatca
     6421 tgttgctaac cggaatgaac aaaggcgtta gcctgccaac cagctccacc ctttcaggat
     6481 tcacacctac ctcctcaaat gcctcccgca gagcggtttg ttccagcgat ccgtcaactt
     6541 cctcccattt gccacccgga aggctcactt gtccgctgtg aagcccttcg taggtttgcc
     6601 ttttgatgag aaccaacctt gtctcgtctt cgtccggaaa gaataggatt agcactgcac
     6661 tttgacgggt ttgcccattt gagctgctat ttccattcac gtttttacga atggagggcg
     6721 acatcaacag ctgtgcctct acaccaggca aaccttggcg cattttaccc ttcaaactgc
     6781 ttataaaatg agaaaacgca catgcagaaa aactatacat agcctgctaa gctccttaaa
     6841 atatcattcc actacaacct atcaaaggta aaaaaattga atttatcatt acccaacatt
     6901 gcgggtatat catatttcct taactttacg ctagaaaggt ttttccatat tggttcatat
     6961 tagtgcagct aaatatgaaa aagataattt taatttcgac tctggccctg ttggttgcca
     7021 gcacagggtg cgtatctaaa aaatacctca agcgaggcat taagtatgag gaagctggac
     7081 tttgggagct agcagccgaa tcgtacatcc aatcgctaac ctcgaaacgt gacaacctgg
     7141 atgcaattgt tgggcttaaa cgaacaggtc agaaagtgat tgatgaaaag tgctacaaat
     7201 caatagccgc ttacgaatcg gacaatttga aggaagctgt atacgactac cttgatgcgg
     7261 tggattttag taaacgaaca tcctcgctag gcgttgagct aatggttaat agtagggcta
     7321 ccgattattt caaggatgcc aaacctagat atattgaaac gttgtatgct caagcccagc
     7381 agcagatgga tgctgaaaag tttcagcaag ctgaagccct gcttgatgag attaagaagc
     7441 tggaacccga ctatggaaac gtgaaggaaa tgctgaaggt atctaaatgt gagcccttat
     7501 accgacaggg gaaacagctg atggaaagtg gttactaccg aaaatcctac ggaaatttta
     7561 accagatagt aaccgaattt ggcagctaca aggatgccgc catgctgatg ggtgatgccc
     7621 tccaaaaagc cattataacc ataaaggtca acaattttac agggggttac tgcactaaag
     7681 ccctgaccca acgcatacaa agttcggtgg ttgcttcagt aaataccctt aacaacccct
     7741 tcgttaaaat tgttgactcc gataatacca tacaaatcat cgaggaacaa caacgtagcc
     7801 ttacacaagg ctcggacatt gaagttggta aaatactggc tgcaaaagca attctaacag
     7861 gcgaggtgct tgaatgtaag caggatgctg gccgtctgaa taaatccgac aagcgaggct
     7921 accttaaaga ggtaaccaag gtgaaaaatc ccgtaacagg tgaaacggag aacaaggtgg
     7981 agtataaaaa ggtttcctac gaggtttaca gcattcagaa ccgggtaact ttctcgctga
     8041 aatttcagct tacatcaacc gagaccggtg ccgtgatggt ttccgatatt gcccgaagcc
     8101 agcaagcaga tgaaaccacc tatgctgtat tcaacggcaa gcccgaaaag cttgtccccg
     8161 gatattggga aaatattaat aaggattcac caaaagattt tgtatccgac aacccgaccg
     8221 atgtttctgc acttcaaaaa atgctacagg cccgcaggga aatatcctca gttgaccagc
     8281 taaccgacaa ggccattgct gccctgtcca aacaggtttc cgaacgaata aaccggtata
     8341 atcccgaaca gtaaaaacta tggttaaaaa cagctactcg acgattgctc ttgcctccat
     8401 catattgttg ctggctgcat gtagcggaac aaaaaaatct gttgatacaa ttccgtcccc
     8461 atcgtgggta caaaatcgcc caacctcctc aatttactac gttggtatag gttctgcccc
     8521 taaaataggc agcccaaacg attaccaaca agctgccaaa caaaatgcac tggctgatat
     8581 ggcaagcgag atttcggtaa agatatcgtc taactccgtg ataagcgcct ttgaaaccca
     8641 gcaaaatttt tacgaggatt actcggcaag catacgtgcc gaagctcaaa aggaacttga
     8701 aggatatgag atggccgaag catgggagga ttcacaaaac tactgggttt actaccgcct
     8761 ttcgaaagaa acctaccgga aaatttcaga tgagaaaaag gcaaaagcag cttcgctctc
     8821 gctcgactac tatgataaag cattgaaaag tgctgaaaat ggtgattaca gaacctgcct
     8881 gctccaggta gttagggcac tggagactct caaaccatac ttcgccgaat cggttactgc
     8941 cagctaccag ggaagggaga tctttctggg aaatgagcta atccagctgc ttaccgaaac
     9001 cctgaataac ctgctgctta acggaccttc cgaggttatg gcacgaatgg gacaaccaat
     9061 tcctgccggc tcgttaacct acagcgttac caatcgtatt ggtattcccc aaaagggaat
     9121 gcctcttagg gcaagctact ccgaaaagcc aatgctgatg aacaagagta ttaccggtag
     9181 tgatggcaaa gcttgttttt cggtcgatcg tgtcagctcc tcacgcaatg tcgaaaaaat
     9241 agccgttgtg ctggacttta gcgcaatagt tcaggaggcc accaccgact ttgacttgcg
     9301 taagcttcta tcccgatttc aggttccttc ggttgaaacc tcaataagaa ttgataagcc
     9361 tacattttcg gtagtaacca gggaaatgct gttcgacaag cccgctcaaa agaacgttct
     9421 ggccgaagcc ataaagcgta aagtcaccga tgagggattc ccgatagccg aaaaaacatc
     9481 cgatgcagat tttgccatta ccattgaggc ctcaactgct aaaaccggag aatcgggcag
     9541 ttacaagcag gcaagccttt cagctttgat atacgttacc aacgcacagg gtgttgaggt
     9601 ttaccgccga agtttagacc ccataaccgg ttctcatttt gactgggagg ctgctggctt
     9661 aagcaccttc gacaaagcgg ccaaacggct tgagagctc
//
LOCUS       DAOSHM010000079         8224 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_7450_length_8224_cov_20.676949, whole genome shotgun sequence.
ACCESSION   DAOSHM010000079 DAOSHM010000000
VERSION     DAOSHM010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 8224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8224
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7450_length_8224_cov_20.676949"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            329..1282
                     /locus_tag="PK839_09490"
     CDS             329..1282
                     /locus_tag="PK839_09490"
                     /inference="COORDINATES: protein motif:HMM:NF024869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HOG73008.1"
                     /translation="MIWQFVLDKLKSGQSMYLIVVSETLGSSPGKVGFKMAVSSDGEI
                     SGSIGGGVMEFRMVELARKELAVGGKNTFLKRQVHSSEAEEDKSGMICSGEQTHIFIY
                     LSTLNLKICSDIVAAISSGKTGLLHIEPKRLEFKSAGVAELPAKHEFNHNGNSWYYSE
                     VIGIKDRAYIFGAGHISLPLTHILSLLDFWVEVYDNRKELVTFTSNNLANKKEVIDFD
                     NASKYVMEGNSTYCLIMTFGHKFDEMILRQLLPLKVGYIGMIGSKSKVKAIFDNLLEE
                     GYSPDQLAKVCSPVGLPIGSQTPAEIAVSIAAQIVQWRNGL"
     gene            complement(1301..1978)
                     /locus_tag="PK839_09495"
     CDS             complement(1301..1978)
                     /locus_tag="PK839_09495"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HOG73009.1"
                     /translation="MKKYLFILVGVAISVFASAQNTDSRDITPFDKLKVIGEVKVYLQ
                     RDSVEHISVKTYDVPTSDIHTEVKGKTLEVALKANIYKSVKADIYVYYRELRDISLSA
                     AASISLNDTLTGDKVVINANTSSEFDGGLNVETADIVVGQGSTVRVRGAVKNYEAKVN
                     TGGVLSALDLVSDSTFVTVSTGAIAKVNASRLIDAKVRTGGSLTYTGNPKQKSINTLM
                     GATIVEQ"
     gene            2266..>2738
                     /gene="msrB"
                     /locus_tag="PK839_09500"
     CDS             2266..>2738
                     /gene="msrB"
                     /locus_tag="PK839_09500"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HOG73010.1"
                     /translation="MVQRIIITIAFFFTIFSTCLGQSKQVEADNKGDQMNEVKQNLTG
                     LQRYVTQQKGTETPFTGEYWNHFEKGVYVCVCCGAELFESETKFHSSCGWPSFFGSKF
                     EENISFNEDSSHGMVRTEVVCKKCGAHLGHIFNDGPKPTGLRYCINSASLKFIPD"
     assembly_gap    2739..2838
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2890..3492
                     /locus_tag="PK839_09505"
     CDS             2890..3492
                     /locus_tag="PK839_09505"
                     /inference="COORDINATES: protein motif:HMM:NF014269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HOG73011.1"
                     /translation="MPISTKQIPIFPLEVFLLPGEELPIRIFEPRYQELVNDLDKTGE
                     SFGIPFVLNEKIMAYGSEVELSNIVARNSLGQMVVIVKGVRNFHLLQYSEAPTSKPYG
                     SGAIEYIDDDFRSTNPELIVLAKKLKLEISENLGILVKDEAISLASVAKGLMMTSEDK
                     YTFYSLRSKTKMENFLIKRLRFIEQIQKQEKLLANNFSLN"
     gene            complement(3479..4810)
                     /locus_tag="PK839_09510"
     CDS             complement(3479..4810)
                     /locus_tag="PK839_09510"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOG73012.1"
                     /translation="MHKRVKFIAAAVFILLGAIFLAFRYDKGVFSKGLTYSGLKYPTV
                     NTLKITNALTSSSEYEWLNNAFSSFIAKWELTGASVAIAKDGRLIYARGFGYASKEEN
                     IPMEPYNLLRVASVSKLITATAVMKLVEENKIQLDSKVFGPNGILNDNIFLNYKDKRV
                     EDITVKNLLNHSAGWSTRWGDHMFINEVVARELHKELPVSLEDIVVFALSKKLHFAPG
                     SHSSYSNLGYAILELVIEKITHTDYETFVQRSIFRPLSIYDARIAQNWDSLRYPNEAR
                     YYEVNEAERVMAFDGSKEGVLKCRGGDDVHTLGAAGGWVISPISLTRFLLAIDGSSSF
                     PDILSKESVAVMTQTSNDKFHPIGWRWVMPGGKLWRSGSLPGTTALAVSSSNGYTYVF
                     ISNTSPWKGARFPYIVDRFFDKLLPGIEGKLPGRNLFFPETSTMGGVASLN"
     gene            complement(4985..6031)
                     /locus_tag="PK839_09515"
     CDS             complement(4985..6031)
                     /locus_tag="PK839_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low specificity L-threonine aldolase"
                     /protein_id="HOG73013.1"
                     /translation="MINKHGFASDNNSGVHPAILQAIMEANQGHTLAYGEDMYTEKAK
                     ALFRELLGTDVEVFFVFNGTAANVLGLQAATQRHNAVICAETAHINVDECGAPERITG
                     CKLLTVKTPDGKLTPSLVSTQMHGFGFQHHSQPKVISISQASELGTVYTPEEVKALSD
                     FAHSHGLYLHMDGTRITNAAVSQSCSLKEISGDAGVDVLSFGGTKNGLMYGEAVVFFN
                     SSIAKDFMYTRKQDLQLASKMRFISAQFIAYLSNNQWATTARHSNQMAQLLADKLRRF
                     SAVKISQKVQVNEVLAIIPPEIIPELQKEYFFYIWDEATSEVRLMTSWDTTEEDIDRF
                     TEILEKLLKKFNML"
     gene            complement(6060..>8224)
                     /locus_tag="PK839_09520"
     CDS             complement(6060..>8224)
                     /locus_tag="PK839_09520"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HOG73014.1"
                     /translation="GAWLFDSSPDSIYKELGQRDNSDNLKSDAFSVYISTYNDGVNYL
                     QFTVSASGVQTDIKVTSDEEDRSWNAVWESEVSMDNRGWYVEMKIPYSALRFSKNDTT
                     VWGVNFLRLLKRYNEKCTWNFVDKSKSGFTTQSGVLVGIAGIKPPPRLSFMPYLSAYA
                     NRYPDKSGTDYQVTGGMDLKLGLSQSFTLDATLIPDFGQVRSDDRILNLSPFEVKYSE
                     ARQFFTEGTELFNKGGIFYSRRVGSTPVDYDKAYESLDTNEVVSDNPSSTKLINASKL
                     SGRTSGGLGIGVFNAVSRKTYAEITDTATGQHRKFLTQGLTNYNMLVFDQSLAHNSYA
                     SIVNTNVFRPDGDYTANVTATDFTIRDKKNKFAVAGVGAFSQIRNDSTSNGYKSSVTL
                     MKTSGNFQGDVWVNVESKHFNPNDLGYLQSPNELSSGVELEYIIFKPFWRLNQLQTSL
                     EYVNQMLYSPRNFVARSISGSIHFITRRYLFGQFYTEVVPQSRYDYYEPRTEGRKLKL
                     PRSIYFNWYGSPDYRKTLALDHQLEYWKADGYNQYGFSYTIAPRIRFSDKLLLVNSWS
                     QSFDYNAIGYFGGSGSNITMGKRDVVTLTNTLSIQFTFSSKSFINLRARHYIRHYKYD
                     SFYTLNLDGTLTPIASPEYSNKNYNLFNVDFYYQWIFAPGSELVFAWKNMEEEMGTIP
                     INSYFDNVDRVFGSPQYNSFSIKFLYYIDYQMIARLAR"
BASE COUNT         2311 a         1841 c         1771 g         2201 t
ORIGIN      
        1 ttctgcctct caaccagggc acctggcact cccatcctta aattcataaa gcatactccc
       61 cccggattat ccatttacta attcctaccc actacgttcc ttttctatac taccacacga
      121 gaggactaac ttcgttgagc tctccgccgg ctggtgggta ggctcgggcg gtagcaggga
      181 atgggctttt aactttaaag ccaatcaatc caactcttca gcatttagga caatgataaa
      241 aaataatgct tcgagtagaa atgaacttga aggctatgaa atttcaacaa atttcaccta
      301 cttagcaggt ctttaaaaaa cggaaaagat gatatggcaa ttcgtgctcg ataagctaaa
      361 gtcgggtcaa agcatgtacc ttattgtggt atccgaaacg ctgggtagta gccctggaaa
      421 ggtggggttt aaaatggccg tttcgtccga tggcgaaata tcaggctcaa taggaggcgg
      481 tgttatggag tttcgcatgg tagagctggc ccgtaaggag ctggctgtag gaggtaaaaa
      541 tacttttcta aaacggcagg tgcatagcag tgaggctgag gaggataaat cggggatgat
      601 ttgctcgggt gagcaaaccc atattttcat ttacctgtcc acgttaaatt tgaaaatctg
      661 cagcgatata gtggcagcca tatcctccgg caaaaccggc ttactccaca tagagcctaa
      721 gaggttagaa tttaagagtg ctggcgtagc agaattgccc gccaaacatg aattcaacca
      781 taacggtaac agctggtact actccgaagt tattggtatc aaagacaggg cttacatttt
      841 tggagccgga cacataagcc ttcccctaac ccatattctt tcgctcctcg atttttgggt
      901 tgaagtttac gataaccgta aggagctggt aacctttact tccaataacc ttgcaaataa
      961 aaaggaggta attgatttcg acaatgcctc aaaatatgta atggagggca atagcaccta
     1021 ctgcctcatc atgacttttg gacataagtt cgacgagatg attctcaggc agctacttcc
     1081 gcttaaggtt ggatacatcg gcatgattgg cagtaaaagc aaggttaaag ccatatttga
     1141 caacctgcta gaagaaggct attcgcctga ccagctggcc aaagtttgct cgccagttgg
     1201 gctaccaata ggtagtcaaa cacccgctga gatagctgtc agcattgccg cccagattgt
     1261 tcaatggcgg aacggactgt aaaatttttc tggaagaggc ctactgctcc actatggttg
     1321 cccccattaa tgtattaata ctcttttgtt tagggttacc tgtataggtc aggctgcctc
     1381 ctgttcgtac cttggcatca ataagccggg aggcgtttac cttggcaata gcgccagtgc
     1441 tcaccgtaac aaacgtgcta tcggaaacca ggtcgagagc cgacaaaact ccgccggtgt
     1501 taactttagc ctcatagttt tttacagcac cccttacacg aactgttgac ccttgcccca
     1561 caacaatatc ggctgtctca acattcaaac caccgtcaaa ctcagagctg gtgttggcat
     1621 ttatgacaac cttatcgcct gtaagagtat cattcaggct tatgctggca gcagctgaaa
     1681 gtgaaatatc ccttaattcc ctgtagtaca cgtaaatatc agccttaaca ctcttgtaaa
     1741 tgttggcctt gagagccacc tccaaagttt ttcccttaac ctctgtatgt atatccgagg
     1801 taggcacgtc gtatgtttta acgctgatgt gctctacgct atctcgttgc aggtaaacct
     1861 ttacctctcc tatcacctta agcttatcga aaggggttat gtccctgctg tcggtattct
     1921 gcgcactggc aaataccgaa attgccacgc caactagaat aaaaagatac ttcttcatag
     1981 gtattaagct tttatgaatt tagtccaaga aataatacat ggtaaaattg ccaatatttt
     2041 tcaatcgaaa cctacttaag atacagaagt ttgccgttat aacctttcac aacttctatg
     2101 caaatgtacc catgctaagt tgtaaaagtg caaactttaa cataaaataa ccaacatgca
     2161 gctattttca ccattacatt ttcgccttgc cgttttaaag taaatcacta gagtgaacaa
     2221 aacagaaaaa tattcgtttt cgtaataagt agtagccaca acatcatggt tcaacggatt
     2281 ataattacca tagctttttt tttcaccatt ttttcaacct gtttaggaca gtcgaaacag
     2341 gttgaagcag acaataaggg tgaccagatg aatgaagtaa aacaaaacct aaccgggttg
     2401 cagcgttacg ttacccaaca gaaaggcact gaaacccctt ttacaggtga gtactggaat
     2461 cattttgaaa agggagtata tgtttgcgtt tgctgcggtg ccgaactttt tgagtcggaa
     2521 accaagtttc actccagctg tgggtggccc agcttttttg gcagtaagtt tgaagaaaac
     2581 ataagcttca acgaggactc cagccatggc atggttagga ctgaggtggt atgtaagaag
     2641 tgtggtgccc atttaggaca catattcaac gatggcccaa agcccaccgg cttgcggtac
     2701 tgcattaact ccgcatcgct taaatttatt cccgataann nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnngc cgataattag gctaccagga atcaaaacct tagctaaaca
     2881 aataaccata tgccaatttc aacaaagcaa ataccaattt tccccctgga agttttcctg
     2941 ctacctggag aggaactccc aatcagaatt tttgaacccc gctaccagga gctggtaaat
     3001 gacctggaca agaccggtga gagttttgga atacccttcg ttttaaacga aaagataatg
     3061 gcctacggaa gcgaggtcga actctcaaac attgtggccc gaaacagcct agggcaaatg
     3121 gtcgtaatag ttaagggggt tagaaacttc cacttacttc agtatagcga ggcgcctaca
     3181 agtaaaccat acggcagcgg ggcaattgag tatattgatg atgatttccg ctccaccaac
     3241 ccggagctaa tcgtgctggc aaagaagctg aaacttgaaa ttagtgaaaa ccttggaatc
     3301 ctagttaaag atgaggcaat tagcctggcg agcgttgcaa aagggctgat gatgacctca
     3361 gaagataaat acacctttta ctcgcttcgc tcaaaaacaa aaatggaaaa ctttttgatt
     3421 aaaaggttga ggtttattga gcaaattcaa aagcaggaaa agctgttggc aaataatttc
     3481 agtttaaact agccacaccg cccattgtgc tggtttcggg aaaaaagaga tttctacccg
     3541 gaagttttcc ctcgatgccc ggcaacagct tgtcaaaaaa cctgtcaaca atatagggaa
     3601 atctagctcc cttccacggg ctggtattcg aaataaaaac ataggtgtaa ccgttgctac
     3661 tcgaaactgc cagtgcagtt gttccgggta aagaacccga acgccaaagt tttccgccgg
     3721 gcataaccca cctccatcca ataggatgaa acttatcgtt gcttgtttgg gtcataacgg
     3781 caactgactc tttcgataga atatcaggga agctgctgct cccatcaata gccagtagaa
     3841 atctggtaag gctgatgggt gaaataaccc atccaccggc ggccccaaga gtatgaacgt
     3901 catcgccacc cctacatttc agaactcctt cctttgaacc atcaaaagcc ataacgcgtt
     3961 ctgcctcgtt aacctcgtag tagcgagctt cgtttggata gcgtaagctg tcccagttct
     4021 gggcaatgcg ggcatcataa atacttaagg gacggaaaat gctacgctgt acaaaagtct
     4081 cgtaatccgt atgggtaatt ttttcaataa caagctccag aattgcgtag cccaggttgc
     4141 tatacgagct gtgggaaccg ggtgcaaaat gtaatttttt ggaaagggca aatactacga
     4201 tatcctccaa tgaaacgggc aactccttgt gcagctcacg ggcaaccacc tcgttgataa
     4261 acatgtgatc gccccaccgg gttgaccatc ccgcagaatg atttaataga tttttaacgg
     4321 taatgtcctc taccctttta tccttgtagt tcaaaaaaat gttgtcgtta agaattccat
     4381 tgggtccaaa aactttggaa tcgagctgaa tcttattctc ctcaacaagc ttcatcacag
     4441 cagtagccgt aattagcttt gaaacacttg ccactcttag aaggttgtat ggctccattg
     4501 ggatattctc ctcctttgag gcgtatccaa agccccgtgc ataaattagc cgtccatcct
     4561 tggctatagc cacgctggca ccggttaact cccatttggc aatgaagctg ctgaaagcgt
     4621 tgttcagcca ttcatactcg ctgctactgg ttaaagcatt ggtaatttta agggtattaa
     4681 ccgttgggta ttttaaacca gaataggtaa gacccttaga gaatacgccc ttatcataac
     4741 gaaacgccag gaaaattgct cccaaaagaa tgaatacagc tgcagcaata aattttaccc
     4801 ttttgtgcat tagctttcaa tgaagttacc cagcggcaaa agtagctatg attagagtaa
     4861 atgacaaaaa aatttacacc cactcacgaa gaccagataa ataagcatgg aaaattaacc
     4921 attcgcaaat ggcaaaacct tgaaaaaggc aattccctgg ttctaaccta tttgtagtgc
     4981 cgaactagag catgttgaat ttctttagaa gcttctcaag aatttcggta aacctatcaa
     5041 tatcctcctc ggtggtatcc catgaggtca tcaggcgaac ttccgaagta gcttcatccc
     5101 aaatgtagaa gaagtactcc ttttgcagtt ctggaataat ttcaggagga atgatggcaa
     5161 gcacttcgtt tacctgcact ttttgcgaga tcttaacggc gctaaacctc cggagcttat
     5221 cagccaacag ctgggccatt tggttggaat gcctggcagt agtagcccat tggttattgc
     5281 tcaggtaggc tatgaactgg gcggaaatga accgcatttt agaagcaagc tgtaaatcct
     5341 gctttcgggt gtacataaaa tccttagcaa tggaggagtt gaaaaacaca actgcctcgc
     5401 catacatcag tccatttttt gtaccgccaa acgaaagcac atcaacaccg gcatcccctg
     5461 aaatctcttt taaactgcag ctttgcgaaa cggcagcatt ggtaatccgg gttccatcca
     5521 tgtgcaggta aagcccatgg ctatgggcaa aatcggaaag tgccttcact tcttcgggtg
     5581 tataaaccgt tcccagctca ctggcctgag aaatagaaat tactttaggc tgagaatggt
     5641 gttgaaaccc gaagccatgc atctgggtgc ttaccaaact aggtgttagc tttccatcgg
     5701 gagtcttaac ggtgagcagc ttgcacccgg taattcgctc gggtgcacca cactcatcaa
     5761 cgttaatgtg ggctgtttcg gcgcaaatta ctgcattatg gcgttgagtt gcagcttgta
     5821 aacccaacac gtttgcagcg gtgccgttga aaacaaaaaa tacctcaaca tcggttccaa
     5881 gcaactccct aaagagcgct ttggcctttt cggtgtacat atcctcgcca taagccaggg
     5941 tatgcccctg gtttgcctcc ataatagcct gcagaatggc tggatgaaca cctgaattgt
     6001 tgtcgctggc aaatccatgc ttgtttatca tatatgtaaa cttaaaaggt tgttaaaaac
     6061 tatctggcta acctagcaat catctggtaa tcaatatagt ataaaaattt gattgagaag
     6121 ctgttgtact gtggacttcc aaaaaccctg tccacattgt caaaatagct attaatagga
     6181 attgtgccca tttcttcctc catgtttttc catgcaaaaa ccaactcgct tccgggagca
     6241 aatatccact ggtagtaaaa atcaacattg aaaaggttgt agtttttgtt agagtattca
     6301 gggctggcaa ttggtgtaag cgttccatcc aaatttaggg tgtagaagct atcatacttg
     6361 tagtggcgta tgtagtgcct tgccctaagg ttgataaaag attttgagct gaaggtaaac
     6421 tgaatgctaa gcgtgttggt aagggtaacc acatctcgtt tacccattgt aatgttgctg
     6481 ccgctacccc caaagtatcc aatggcatta tagtcgaacg attgcgacca tgagtttacc
     6541 agcagcagct tatcgctaaa gcgaatccgg ggagcaatgg tgtaggaaaa tccgtattgg
     6601 ttgtagccat cggctttcca gtactccagc tggtggtcga gtgcaagagt tttccggtaa
     6661 tctggcgaac cataccagtt aaagtatata ctacgcggca gcttgagctt tcggccctcg
     6721 gtcctaggct catagtagtc gtacctggat tggggaacaa cttcggtgta gaattgacca
     6781 aacaaatacc gtcgggtaat aaaatgaata ctacccgaaa tagacctggc cacaaaattt
     6841 ctgggtgagt atagcatctg gtttacgtat tccagggatg tttggagctg gtttaatcgc
     6901 caaaagggtt taaaaataat gtactccagc tccacaccgc tcgaaagctc gtttggactt
     6961 tgcaggtatc ccaaatcgtt ggggttaaag tgtttacttt caacgttcac ccaaacatct
     7021 ccctgaaagt taccgcttgt tttcatcagc gtaaccgacg atttatagcc atttgaagtc
     7081 gaatcatttc taatttggct aaaagcgcct actcccgcca ctgcaaattt atttttctta
     7141 tccctgatgg taaagtcggt tgcggtaaca ttagcagtgt agtcgccatc gggtctgaaa
     7201 acgttggtat tgactatact tgcgtaggag ttatgggcta gcgactgatc aaaaaccagc
     7261 atgttgtagt tggtaagccc ttgggttaaa aacttcctgt gctgcccggt agctgtgtcg
     7321 gtgatttcgg cgtatgtttt acgggatact gcattgaaaa cgccaatacc caatcctccg
     7381 gatgttcgcc cggaaagttt gctggcatta atcagcttgg tgctggatgg attatcgctt
     7441 acaacctcgt tggtatcgag gctttcgtaa gccttatcgt aatcaaccgg agtgctgcca
     7501 acccttcggg agtaaaaaat tcccccctta ttgaatagtt ctgtaccttc ggtgaagaac
     7561 tgcctggcct cgctgtactt tacctcaaag ggagaaaggt tgagtattct atcgtcggag
     7621 cgaacctgcc caaaatcggg tataagggta gcatcgagag taaaactctg gctaaggccc
     7681 aacttcaagt ccattcctcc ggtaacctgg taatcggtac cactcttgtc gggatacctg
     7741 ttggcgtaag ctgaaaggta gggcataaaa gacaacctgg ggggtggctt aattccggca
     7801 attccaacca gcactcccga ctgtgtggta aatcccgatt ttgatttatc aacaaagttc
     7861 caggtgcatt tctcattata cctttttaag agtcgcaaaa aattaacacc ccatacagtt
     7921 gtatcatttt ttgaaaaccg gagggctgag tagggaattt tcatctccac gtaccaaccc
     7981 cggttgtcca tgctaacttc gctttcccaa acggcattcc atgagcgatc ctcctcgtcg
     8041 gaggtaacct tgatgtcggt ttggacgccc gaagccgaaa cggtaaattg aaggtagttt
     8101 accccatcgt tataggtgct gatgtaaacg ctaaaggcat cggacttgag gttatcgcta
     8161 ttgtcccttt gcccaagttc cttgtaaata ctatcgggcg atgaatcgaa aagccatgca
     8221 ccta
//
LOCUS       DAOSHM010000080         8190 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_7497_length_8190_cov_4.215243, whole genome shotgun sequence.
ACCESSION   DAOSHM010000080 DAOSHM010000000
VERSION     DAOSHM010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 8190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8190
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7497_length_8190_cov_4.215243"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            110..583
                     /locus_tag="PK839_09525"
     CDS             110..583
                     /locus_tag="PK839_09525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG73015.1"
                     /translation="MKKNYRLDKSFGPTGVIAGIILLIVGIILTPFYVSGLFLILIGS
                     FVGLSSTSTIVDYENKRMKFSNNLFGIISVGKWVAIENDMKVGIIKSKKTWRSYSRGN
                     RTLDITNNDFRILLYDSENRQVMPIKKVDTMDIAKKEMKEIAENLGLSSNNSLVN"
     gene            1044..1904
                     /gene="hisG"
                     /locus_tag="PK839_09530"
     CDS             1044..1904
                     /gene="hisG"
                     /locus_tag="PK839_09530"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005789131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HOG73016.1"
                     /translation="MNTKITLAIQKNGRLNEGTIELLNRCGIKISNGTNHLKAFAEGF
                     PLEVLLLRDDDIPQYVADGVADTGILGYNEVLEKGKDIAVVCQLGFSRCRLSIAVPRD
                     VRYSGRQWLSGKRIATSYPKILKAFLNENAISADIHTISGSVEITPGIGMADAIFEIV
                     SSGGTLLSNNLKEVDVIMKSEAVLVANKNLNGQKNEFLERLEFRIEAVRKAQRNKYIL
                     LNAPKDKLNEIISLIPGIKSPTVLPLAAEGWCSLHSVVNEDDFWEVIGKLKAAGAEGI
                     LAVPIEKMVI"
     gene            1917..3194
                     /gene="hisD"
                     /locus_tag="PK839_09535"
     CDS             1917..3194
                     /gene="hisD"
                     /locus_tag="PK839_09535"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009120500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HOG73017.1"
                     /translation="MKTITDNLNISYSELLKRPAENNLEIKNKVQEIIEKVKVQGDAA
                     LKSLTKKFDGVDIDSFEIDKQTIANSQKQIDGNIKDAIELASRNIMKFHSAQKTKDVE
                     VTTMPEVSCKLVYRPLERVGLYVPGGTAPLISTVLMLGVPARVAGCEELIVCTPPPVN
                     PFVLYACYLVGAKVYTAGGAQAIAAMAFGTETIPRVDKIFGPGNSYVTEAKMQVAAMG
                     VPIDMPAGPSELLVIADDSAVPQFVAADLLSQAEHGFDSQVVLISTSKTIINKTLDEI
                     SQQLQNLPRKNIAVKTLENSYAIKVETIEKAIEISNIYAPEHLILAVACPEKFETSIK
                     NAGSIFIGNYTPESAGDYASGTNHTLPTSGFARNWSGVNLLSFTKTITYQTITPQGLQ
                     ELGNYIVELANAEKLTAHANAVKLRMEKISKQS"
     gene            3199..4260
                     /gene="hisC"
                     /locus_tag="PK839_09540"
     CDS             3199..4260
                     /gene="hisC"
                     /locus_tag="PK839_09540"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HOG73018.1"
                     /translation="MMTLKETLDMLRPNIAGLKPYSSARNEFPGSDAILLDANENPYN
                     PRYSMLAVNRYPDPFQRKAKEKIAGIKGVRPEQIFLGNGSDEAVDLIIRCFCEPSTDS
                     IAIFAPTYGMYAVCAAINNVGVKQFLLNNDFSININQFFNGLSPNIKVVFICSPNNPS
                     GNTQSLESIEQVVSRYRGIVVVDEAYIDFCPEKTSINLITRYPNIIVLQTFSKAWAMA
                     GARIGVAFANQLLIDTLSKVKFPYNIGLPSLRILDKAIEKSSSSKIYINKIIDEREKV
                     RLKLQQLPFVKMVYPSDSNFLLVKATDANAIYKHLLGNGIVVRNRSHEPLCENCLRIT
                     IGRPDENQKLMQTLITYQP"
     gene            4257..5339
                     /gene="hisB"
                     /locus_tag="PK839_09545"
     CDS             4257..5339
                     /gene="hisB"
                     /locus_tag="PK839_09545"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005846279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HOG73019.1"
                     /translation="MKPILFIDRDGTLIKEPSDFQLDSFDKLDFMPGVFTWLGQICRE
                     FDYYLVMVTNQDGLGTPSFPEENFSAPHQIMLKALEAEGIRFDEILIDRSFPDEKLPT
                     RKPGIGMIKHLLNGDFDTVNSFVIGDRETDEQFALNIGCRAIRFGSEAAIPDNLTAFS
                     WEAVYHHIKPKRIASFSRRTNETEVTVSINLDGVGKAKVNTGIGFFDHMLALLAKHSG
                     IDIEIDCNGDLHVDEHHTIEDVGITLGETLRKAIGNKVGISRYGFALPMDESKAMVLV
                     DYSGRPYLVFKGSFTCDFVGLMPTEMVKHFFHSLAYSALITLHIEFEGENNHHKIEAI
                     FKCFAHSLASAIKKEGANIPSTKGIL"
     gene            5351..5947
                     /gene="hisH"
                     /locus_tag="PK839_09550"
     CDS             5351..5947
                     /gene="hisH"
                     /locus_tag="PK839_09550"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HOG73020.1"
                     /translation="MRKISTVGIIQYGAGNQASIINALESLGVTSLPIVTPNDVGRAD
                     KLIIPGVGHAMKAMNQLKSSELDEAIIKTNMPLLGICLGMQLLGIKSDEGKAKCLSVC
                     DFETVKFNIPLKVPQMGWNKVKLRENRLFYGLPQEEWFYFVHSYYIPKSMYTIATCSY
                     GVGFTAAVQNKNFWGVQFHPEKSGKKGLQILKNFIELC"
     gene            5941..6657
                     /locus_tag="PK839_09555"
     CDS             5941..6657
                     /locus_tag="PK839_09555"
                     /inference="COORDINATES: protein motif:HMM:NF013168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]
                     imidazole-4-carboxamide isomerase"
                     /protein_id="HOG73021.1"
                     /translation="MLVVPAIDIINGKCVRLFQGNFSKVKEYGLNPVEQAKAFADMGF
                     QHLHLIDLDGAMSGSSRNFNILEQIVKSTSLKVDFGGGLRNVSSITDALSAGAWKVNI
                     GTLLLKNEELVNNHLPIEKIIGAVDCEYSIVKTNGWKKQTSITVDEFLTNLVGIGFSH
                     FTITDITRDGTQTAPAFGLYEELRQKFSTITLWASGGVSSLSDIFNLKKVKVDGAIVG
                     KAYYENSLNLKKILRCLQNE"
     gene            6639..7394
                     /gene="hisF"
                     /locus_tag="PK839_09560"
     CDS             6639..7394
                     /gene="hisF"
                     /locus_tag="PK839_09560"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HOG73022.1"
                     /translation="MLAKRIIPCLDIKNGRTVKGVNFENLADAGDPVMLAERYYQDSA
                     DELVLLDISATLEGRETFIDVVHSVAKAIAIPFTVGGGISSIGQAEKLIKAGADKITI
                     NTAAIQKPELISNLADYFGSQCVVVAIDTMLTEKGWEVVSHAGTKRTGIKALELAKTC
                     EILGAGEILLTSHTSDGTKEGFAIDITRKISQSIGIPLIASGGAGLPEHFAEVLTKGM
                     ADAALAAGVFHYGLLSILQVKEYLLSKNIIVRY"
     gene            7410..8069
                     /gene="hisIE"
                     /locus_tag="PK839_09565"
     CDS             7410..8069
                     /gene="hisIE"
                     /locus_tag="PK839_09565"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006797604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HOG73023.1"
                     /translation="MTNLNFDKLNGLLPAIIQDLSTGKVLMLGFMNREALEVTRKTGF
                     ATFYSRTRRQIWTKGETSGNFLKVLEIIPDCDGDTLLVKVSPQGPVCHTGSDTCFNEQ
                     NTSTPGFIEYLERIIENRKQASVEQSYTAKLFLAGAKRIAKKVGEEAVELALEAENGS
                     DERFLDEAADLIYHTQVLLSSRNLSFERVIETLKARHAKLKVSDKEVENFKRGEKQWG
                     K"
BASE COUNT         2719 a         1583 c         1750 g         2138 t
ORIGIN      
        1 tgataccatt aaaattggtg tttttatacg acgatgtagt attgatatgt aagttggctg
       61 ctattgttcg aagacacaga gttattgttc aataagtaaa gcctaagaaa tgaaaaaaaa
      121 ttatagacta gacaaatcat ttggtccaac tggagttatt gctggaatca ttttacttat
      181 tgttggaata atactaactc cattttatgt atctggattg tttttaatcc tgataggttc
      241 ttttgtaggt ctatcttcga cgagtacaat agtggattat gaaaacaaaa gaatgaaatt
      301 ctcaaataat ttatttggaa ttataagtgt tggcaaatgg gttgctattg aaaacgacat
      361 gaaagtaggt attataaaat caaagaaaac atggagaagc tacagtcgag gtaatagaac
      421 actagatatc actaataatg attttagaat acttctttac gactctgaaa atagacaagt
      481 aatgccgata aaaaaagtag atacaatgga tatagcgaaa aaagaaatga aagagattgc
      541 tgaaaatctt ggattatctt cgaataattc attagttaat tgattaataa tacattacaa
      601 cagcagccaa catgtggtta agctcactgg ggctgatttg ctgacaagaa ttttcattac
      661 ctttaactaa cctaaattca agtaattaat gcaacttttt aagggggtca ggaaagtaaa
      721 aaacaaactt tttcaagaca aagaagaaaa aagcagcttt acacactttt tggggacaat
      781 ataaagtgtt ttaaccctaa tatttaacct aatatttgga ttctaataaa atatttcttt
      841 tatttgcaaa atatcaatcc gtaaatgata gctactagct ataccacagt tcatcatcat
      901 caccattcac attcttggcg aatggcagat gaacattggg tataaccaat agttaatcca
      961 taaagagact actttttaaa gggtctgcca ttcgaaaggc agaccctttc tgctttttgg
     1021 aaatcaccaa aacaaataac gatatgaaca caaaaattac actggctatc caaaaaaatg
     1081 gccgactaaa cgaaggaaca atagagctgc taaaccgctg cggtataaaa atttcaaatg
     1141 gaaccaacca tctaaaagca tttgctgagg gatttccact ggaagtgcta ttactaagag
     1201 atgatgatat accacaatat gtagccgatg gagttgccga tacgggtatt cttggttaca
     1261 acgaggttct tgaaaaagga aaggacattg ctgttgtttg tcagcttggc ttttcccgat
     1321 gtcggctttc cattgcagtt ccacgcgatg ttaggtatag cggcaggcaa tggctatcgg
     1381 gaaagcgaat agcaacctcg tatccaaaaa tccttaaagc tttcctgaac gaaaatgcta
     1441 ttagtgccga tattcatacc ataagcggat cggtagagat aacgcccggg ataggaatgg
     1501 ctgatgctat attcgaaatt gtgagtagcg gaggaacatt gctaagtaat aaccttaaag
     1561 aggttgatgt gataatgaaa agcgaggctg tactcgtagc caataaaaac ctgaatgggc
     1621 aaaaaaacga gttccttgaa aggcttgaat tccgaatcga agcggttcgt aaagcgcaac
     1681 gcaacaagta cattctgttg aatgcaccta aggataagtt aaatgaaatc atcagcctga
     1741 taccgggcat aaagagcccc accgttttgc cattggccgc tgaaggttgg tgctcattgc
     1801 actcggtagt caacgaggat gacttctggg aggttattgg gaaacttaaa gcagcaggtg
     1861 ccgaaggcat tctagccgtg cctattgaaa aaatggtaat ctaaaataat acagctatga
     1921 aaacaataac tgataaccta aacattagct actccgaact attaaagcgg ccggcggaga
     1981 ataacttaga aattaaaaat aaagtacagg aaattattga aaaggtcaaa gttcaaggcg
     2041 atgctgcatt aaagtcgcta accaagaaat ttgatggcgt agatatagat agctttgaaa
     2101 ttgataagca gaccattgct aatagccaaa aacaaattga tgggaacata aaagacgcaa
     2161 tagaattagc ctcaagaaat attatgaagt tccactcagc acaaaaaaca aaagatgttg
     2221 aagttactac aatgcccgag gtaagctgca aactcgttta ccgaccgtta gaacgtgtag
     2281 gcctttacgt acccggtgga acagccccct tgatatcaac tgtacttatg ctaggcgttc
     2341 ctgcaagggt ggctggctgc gaggagctga ttgtatgcac tcccccaccg gtaaacccct
     2401 ttgttcttta tgcatgttac ctagtaggag caaaagttta cacagcaggt ggtgctcaag
     2461 ccattgctgc catggccttt ggaacggaaa ctattcctag ggttgataag atttttggtc
     2521 ccgggaacag ctatgtcacc gaagctaaaa tgcaggttgc ggcaatgggt gtccctatcg
     2581 atatgcccgc gggcccatcg gagttacttg taatagccga cgatagcgct gtaccgcaat
     2641 tcgttgctgc cgatctgctg tcgcaggcag aacacggttt cgatagccaa gtagtactca
     2701 ttagcacaag caaaactatt attaacaaga cccttgatga aatttcccaa cagctacaaa
     2761 atctcccacg aaaaaacata gcggtaaaaa cgcttgaaaa cagctatgcc attaaagtgg
     2821 agacaattga aaaagcaata gaaatcagca acatttatgc acctgaacat ttaattcttg
     2881 cggttgcctg tcctgaaaaa tttgaaacga gcataaagaa tgcaggatca atattcatag
     2941 gaaattatac acccgaatcg gcaggcgatt acgcaagcgg aaccaatcac acattaccta
     3001 caagcggatt tgccaggaac tggagtggag taaatttact gtcgttcaca aaaaccatta
     3061 cctatcaaac aattacaccc caaggattgc aagagctggg taactacata gtagaactcg
     3121 ccaatgctga aaagctgaca gcccatgcca atgctgtgaa gttaagaatg gaaaaaatca
     3181 gtaaacaaag ttaaagcgat gatgacacta aaagaaacat tagatatgct gcgtcctaat
     3241 atagctgggt taaaaccata ttcatcggcg cggaatgagt ttcccggaag cgatgctatt
     3301 ttgctcgatg ctaacgaaaa cccctacaac ccccgataca gtatgttggc tgttaaccgc
     3361 taccccgacc cttttcagcg taaagcaaaa gaaaaaatag caggtatcaa aggagttagg
     3421 cctgaacaaa tttttcttgg aaatggaagt gatgaagcag ttgacctcat tatccgttgc
     3481 ttctgcgaac catctactga cagcatagcc atatttgcac ccacctacgg aatgtatgcg
     3541 gtttgtgctg caattaataa tgttggggtt aaacagttcc tgctcaacaa tgattttagt
     3601 atcaacatta accaattctt taatggatta tcacccaata ttaaagtggt ttttatttgc
     3661 agcccaaaca acccctcggg caatacccaa tcacttgagt caattgaaca agttgtatca
     3721 cgatatagag gtattgtagt ggttgatgaa gcctatatag atttctgtcc tgaaaaaacc
     3781 tccattaatc ttataacccg atacccaaac attattgttc tgcaaacctt ttcaaaggca
     3841 tgggctatgg cgggagcgcg aattggtgtt gcctttgcca atcagctttt aattgacaca
     3901 ttaagcaaag tcaaatttcc ctataacatt gggcttccat cactaagaat tcttgataag
     3961 gcaattgaaa aatcttcatc gtccaagatc tacataaata aaataattga cgaaagggaa
     4021 aaggtaagat tgaaactgca acagctgcca tttgtaaaaa tggtttaccc ttcggattca
     4081 aactttttgc tggtaaaggc taccgatgcc aacgcaatct ataagcattt actaggcaat
     4141 ggaatagtgg ttcgcaaccg cagccatgaa cccttgtgcg aaaactgctt acgaataacc
     4201 atagggagac ccgatgaaaa tcaaaagctg atgcaaacct taataacata ccagccatga
     4261 aaccaatcct atttatcgac cgcgatggaa cgctcattaa agagccatct gattttcaac
     4321 ttgatagctt cgacaagtta gattttatgc cgggagtatt cacctggtta gggcaaatat
     4381 gcagggaatt tgattactat ctggttatgg taaccaacca agatgggctg ggaaccccaa
     4441 gttttccgga ggaaaacttt agtgccccgc accagatcat gctcaaagcc cttgaagccg
     4501 aaggcatccg atttgacgag atattaattg accgctcgtt tcctgacgag aaactaccta
     4561 ctcgcaaacc gggcattggc atgattaagc atctactaaa tggcgatttt gatactgtaa
     4621 actcctttgt aattggcgac agggaaaccg atgaacaatt tgctttaaac attggatgcc
     4681 gagctattcg atttggtagc gaggccgcca ttcctgataa tctaacagct tttagctggg
     4741 aagcggtata ccaccacata aaacccaaaa gaattgcttc gttctcccgt aggaccaatg
     4801 aaacagaagt cactgtaagt ataaaccttg atggcgttgg gaaagcaaag gtaaatacag
     4861 gcattggatt cttcgaccac atgcttgcgc tgcttgctaa acactcagga atagatattg
     4921 aaattgattg caacggcgat ttgcatgttg acgagcacca taccattgag gatgtgggta
     4981 ttactcttgg agaaacgtta cgaaaggcta taggcaataa agttggcata tcgcgatacg
     5041 ggtttgcctt acctatggac gaaagcaaag ccatggtgtt ggtagactat agtgggcggc
     5101 catacctggt attcaaaggc agttttacct gcgattttgt ggggctaatg ccaaccgaaa
     5161 tggttaagca cttttttcac tcgctcgcct actccgcttt aataacatta catattgaat
     5221 ttgaaggaga gaataaccat cacaaaattg aagccatatt taaatgcttt gcacacagct
     5281 tggcatcagc cataaaaaaa gaaggagcga atattccaag cacaaagggt attttataaa
     5341 accaaagtaa atgagaaaga tttcaacagt aggcatcatt cagtatggag caggtaatca
     5401 ggcctcaata attaatgcac tcgagagttt gggcgttaca agtttaccca tcgtaacccc
     5461 aaacgatgta ggaagagctg ataagctgat tattcctggt gtagggcatg caatgaaagc
     5521 tatgaatcaa cttaaaagtt ctgaacttga tgaggcaatt ataaaaacga atatgccttt
     5581 gctgggcatt tgcctgggta tgcaactact agggattaaa agcgatgaag gaaaggctaa
     5641 gtgcctatcg gtttgcgatt ttgaaacggt taagtttaac ataccgctaa aagttccaca
     5701 aatgggttgg aataaagtaa aactcagaga gaataggcta ttttacggct tgccacaaga
     5761 ggaatggttc tactttgttc atagctacta tatccctaaa agtatgtaca ccatcgccac
     5821 atgcagctat ggtgttggat ttacagccgc tgtgcaaaac aaaaacttct ggggagtgca
     5881 atttcaccct gaaaagagcg ggaaaaaagg attgcaaata cttaaaaatt tcattgagct
     5941 atgttagtgg tacctgcaat tgacataatt aatggaaaat gcgtcaggct tttccaaggc
     6001 aacttttcga aggttaagga atatggccta aaccctgttg agcaagcaaa agcttttgcc
     6061 gacatggggt tccaacacct acaccttatt gaccttgatg gcgctatgag cgggtcgtca
     6121 agaaacttca atattttgga gcaaatagta aaatccactt cattaaaggt tgattttgga
     6181 ggaggattaa gaaatgtttc gtccataacg gatgctctaa gtgcaggtgc atggaaggta
     6241 aatattggca cactgctttt aaaaaatgaa gaactagtca acaatcacct acctatcgaa
     6301 aaaattattg gagcggtgga ttgtgaatac tctatagtaa agactaatgg atggaaaaag
     6361 caaacttcta ttacggttga tgaattttta accaatctgg taggaattgg cttttctcac
     6421 ttcaccatta ccgatataac ccgcgacgga acacaaactg cacctgcttt tggtctttat
     6481 gaggaactac gacaaaagtt ttcaacaatt accctatggg caagcggagg ggtgagctcg
     6541 ctttccgata ttttcaacct gaaaaaggtt aaggttgatg gagcaatagt tggaaaagca
     6601 tactacgaaa acagcttgaa cctaaaaaaa attttaagat gcttgcaaaa cgaataattc
     6661 cctgccttga catcaagaat ggacgaaccg ttaagggtgt aaattttgaa aatttagccg
     6721 acgcggggga ccctgttatg cttgctgaaa ggtactacca agatagcgcc gatgaactag
     6781 ttttgctcga tatttcggca acccttgagg ggagagaaac tttcattgat gttgtgcaca
     6841 gtgtggctaa agccatagcc attcctttta ccgttggagg ggggattagt tcaataggac
     6901 aggccgaaaa gttaattaaa gcaggcgccg ataagataac gataaacacg gcagcaattc
     6961 aaaaacctga acttattagc aatcttgccg attactttgg ctcacagtgt gtggttgtag
     7021 caatcgatac aatgcttacc gagaaaggat gggaggtagt tagccacgcc ggaacaaaaa
     7081 gaacaggcat aaaagccctt gaactggcaa aaacctgtga aatacttgga gcaggcgaaa
     7141 ttctccttac ctcacatact tccgatggaa caaaagaagg atttgcaatt gacatcaccc
     7201 gaaaaatttc tcaaagtatt ggtattccac tcattgcgtc gggcggagca ggattaccgg
     7261 aacatttcgc cgaagtatta accaaaggta tggccgatgc tgcacttgct gcaggtgtgt
     7321 ttcactatgg cttgctttcc atcttgcaag tcaaagaata cttactaagt aaaaacataa
     7381 tcgttcgata ctaacaaaaa caaacagcta tgactaattt aaattttgac aagctcaacg
     7441 gcttgctacc cgcaattatt caggatttat caacaggcaa agtgctaatg cttggcttta
     7501 tgaaccgcga ggcattggaa gtaacacgca aaacgggttt tgccacattc tacagtcgaa
     7561 cacgccgaca aatctggact aaaggtgaaa catcaggaaa ctttttgaaa gtgctggaaa
     7621 taattcccga ttgcgatggc gataccctgc tggttaaggt ttcgccacaa ggacctgttt
     7681 gccacaccgg aagcgacact tgttttaacg agcaaaacac tagcacaccg gggtttattg
     7741 aatacctgga aaggataatc gagaaccgca aacaagcgag cgtagagcaa agctatacag
     7801 ccaaactatt tttagcagga gctaaacgca tagccaaaaa agtaggcgaa gaggcagttg
     7861 aacttgcact tgaggccgaa aatggctctg atgagcgatt tctcgacgaa gcggccgatt
     7921 tgatatacca cacgcaggtt ttgctctcga gccgtaatct tagtttcgaa agagttatcg
     7981 aaaccctcaa agcgcggcac gccaaattaa aggtaagcga taaagaagtg gaaaacttca
     8041 aacgtggcga aaaacaatgg ggcaagtaaa aataggttat ggcgttttat tttttaaaag
     8101 taaggattaa ttttgcgctt tgaaaaattg aagccggtct ggtagctcag ctggatagag
     8161 caacagcctt ctaagctgtg ggtcgcgggt
//
LOCUS       DAOSHM010000081         8133 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_7574_length_8133_cov_19.097425, whole genome shotgun sequence.
ACCESSION   DAOSHM010000081 DAOSHM010000000
VERSION     DAOSHM010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 8133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8133
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7574_length_8133_cov_19.097425"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..987
                     /gene="queG"
                     /locus_tag="PK839_09570"
     CDS             <1..987
                     /gene="queG"
                     /locus_tag="PK839_09570"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HOG73024.1"
                     /translation="YYHPYAIFEPIISVKGVLMAMIPLEIVRKLALDVGFSDCGVSPA
                     GKLGDAGNGLLSWLGKGYHGEMAYMERNLNLRLNPSALVPGAKSVISVILAYGHQEIP
                     LMAHPPRVSRYALMPDYHKVMKTMLFRLLNVLREEFGPVNGRPFVDSAPVLERAWAEN
                     SGMGWIGKNSNFIHPKFGSFVFIGELIVDAAVEDSSRQHKNRCGSCTRCMEACPTHAI
                     VEPGVVDARLCISYLTIEKRSPLTEKECGSLNGWCFGCDICQEVCPWNQKLLPITNEE
                     VSIIPELKQLNAGNMMGMTQTQFEEWFGITPLKRAGYEKLMQSVSCAVKASH"
     gene            complement(1023..2438)
                     /gene="ahcY"
                     /locus_tag="PK839_09575"
     CDS             complement(1023..2438)
                     /gene="ahcY"
                     /locus_tag="PK839_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HOG73025.1"
                     /translation="MEQHGNLQPYKVKDIGLAEWGRKEIEIAEKEMPGLMSLRQKYGS
                     QKPLNGARITGSLHMTIQTAVLIETLVELGAQVRWASCNIFSTQDHAAAAIAAAGIPV
                     FAWKGETLEDYWWCTAQALSFPNGKGPNMIVDDGGDATLLVHWGYKAENDRKFVERKA
                     QNHEEEVILNLIRETLQQEPNKWHNLAAELKGVSEETTTGVHRLYQMKEKNELLIPAI
                     NVNDSVTKSKFDNLYGCRESLADGIKRATDVMIAGKVVVVCGYGDVGKGCARSMRSYG
                     ARVIVTEIDPICALQAAMEGFEVKTVDDALAEGNIFVTATGNCDIITLEHMQQMKDQT
                     IVCNIGHFDNEIQMDRLNTQKGVRKVNIKPQVDQYYFPDGHSIFILAEGRLVNLGCAT
                     GHPSFVMSNSFTNQTLAQVELWTKHLAVDVYRLPKQLDEEVARLHLEQLGVKLTTLTP
                     KQADYIGVNPLGPYKSDHYRY"
     gene            complement(2462..3439)
                     /locus_tag="PK839_09580"
     CDS             complement(2462..3439)
                     /locus_tag="PK839_09580"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004162.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HOG73026.1"
                     /translation="MIIVQKPEQTLGKEVALTMGFFDGVHAGHRTLIERVLQVSGLRN
                     LGSAVLTFWPHPRLVLQKDPEKLRFLTTLTEKSKIVSKLGVDFLIIKEFTPDFYNMEA
                     EDFIRHLVKYYHVKHFVVGSKHRFGKGGRGDTALLHELSAELSYTLEVVETLQVDGQG
                     ISSTKIREALLSGNVETANQMLGYPYLITGSVESGKKVGREIGFPTANIRPNDPLKLI
                     PREGVYAVVVSIEGSIRFGMMNIGFRPTVDTMRKQTIEVNIFDFDDEIYFSGIEVAII
                     ARLRNEKRFPSIKELSEQLAIDRIDALNILQTLNPEVFQNLFLTLRPSK"
     gene            complement(3828..4547)
                     /locus_tag="PK839_09585"
     CDS             complement(3828..4547)
                     /locus_tag="PK839_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008660884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOG73027.1"
                     /translation="MDESKVIEISNANIFQEDNLILSDVNLSLAQGEYLYIIGRVGSG
                     KTSLIKTITAELPLKSGEATVCGFNLRTIKRRHIPKLRRKIGVVFQDFQLLSDRSVYD
                     NLLFVLRSTGWKDKQAIKQRITEVLEMVGLKFKDYKLPHQLSGGEQQRVVIARALLNN
                     PEIILADEPSGNLDMESSEGIMQILHSIAESGKSVVMATHNHQLIKKFPARTLKCEKG
                     KLLEIEQMGEIDFNSLMDMDG"
     gene            4783..5499
                     /locus_tag="PK839_09590"
     CDS             4783..5499
                     /locus_tag="PK839_09590"
                     /inference="COORDINATES: protein motif:HMM:NF015949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF364 domain-containing protein"
                     /protein_id="HOG73028.1"
                     /translation="MSTSDKNDILSVLYEAIPAGNKQGEDIFTFGSVYVAASTSEGNI
                     GVCATLGVTVPCFNPAYFNIDRVADRVALNALINARVNYSVLYDGAADIFDVVDFTRY
                     QSIVMIGYFRSLVDKLSSSGVETFIFDLNQHDVPVMPIKLQQEYLGRADCVIVSSTSI
                     SNGTFNGLIVNTPASTNVYMLGPSTPMCDVMFSYPQVKGLFGSVFEPHDQKTIQIIAA
                     GGGTRQFKTCMRKVYRLRHE"
     gene            5553..7133
                     /locus_tag="PK839_09595"
     CDS             5553..7133
                     /locus_tag="PK839_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008451335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HOG73029.1"
                     /translation="MEEEYISIKEASEWASEHLGKKVTTSNISYLIQYGRIAKIGDNG
                     AARISKKDLADYYNSFNGKRQISWKSELGDDLNWALSFDQYKEAETTKHVHRLHPYKG
                     KFIPQLVEYFIDSHTDSFKREVFFKKGDIILDPFSGSGTTMVECNELGMHAIGIDISE
                     FNSLIANCKVTKYNLPDVQIEINRITSVLKGFLQNSKTIEFEEKLLEALKDFNNLYFP
                     VPEFKLKLRKGEVDEQKYGKEKEVEFMPYYEKLVKDYGIILRQDKEDTFLDKWFSQHI
                     RNEIELVFNEIKKIQNRDTKKIISIILSRTMRSCRATTHADLATLLEPVTTTYYCAKH
                     GKICKPLFSILKWWQTYTKDTVKRLVEFDKLRTPTIQTCLTGDSRSIDITEELSKKNR
                     DFAHQVATQKISGIFSSPPYVGLIDYHEQHAYAYDLFGFKRRDELEIGPLYKGQGKDA
                     KDNYVSGISEVLNNAKRFMVDDFNVFLVANDKYNLYPQIAQNAGMKIVNTYKRPVLNR
                     TEKDKNPYSETIFHLKKI"
     gene            7133..7912
                     /locus_tag="PK839_09600"
     CDS             7133..7912
                     /locus_tag="PK839_09600"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF021067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TdeIII family type II restriction endonuclease"
                     /protein_id="HOG73030.1"
                     /translation="MAINNQKKEEIKNIIKESIRSKFKHYKPETLSMPFHYRLLGKDR
                     MALYSFIQSLNTTFGTSIFEPVAVALAQDKFTKVRAQYSVGNTISQGCQQAIQTIMNS
                     LSTGKAPCKPKELEAIRNAALAGNLVKIKTVKVDLFLEDRNGEQYLFDLKTAKPNISN
                     FKDFKRTLLEWAGIIYTSNSNADVNTLIAIPYNPYEPKPYERWTIKGMLDDKYELMVG
                     EEFWNFLGGDDAYNDLLDCFEIAGIELRPEIDGYFSNFTRQ"
BASE COUNT         2303 a         1716 c         1815 g         2299 t
ORIGIN      
        1 tattatcatc cttatgctat ctttgaaccc attatatcgg tcaaaggagt tttgatggcg
       61 atgatacctt tggaaatagt caggaaactg gctttagatg tgggttttag cgattgcggt
      121 gtatctcctg ccggaaagct tggcgatgcc ggcaatgggt tgctaagctg gctgggaaag
      181 gggtatcatg gcgaaatggc ctacatggag cgaaacctca accttcgtct taacccttcg
      241 gcattggtac cgggggcaaa atcggtaatc agcgtgattc ttgcctatgg tcatcaggaa
      301 atacccctta tggcccaccc accaagagtt tcccgctatg cacttatgcc cgattaccat
      361 aaggtaatga aaaccatgct atttagactt ctcaatgtct taagggagga gtttggccca
      421 gtaaacggaa ggccctttgt tgactctgca ccagtgctcg agcgtgcatg ggctgaaaac
      481 tctggcatgg gttggatagg caagaactcc aacttcatac atcccaaatt tggctcgttc
      541 gtgtttattg gtgagctcat tgttgatgca gcagttgaag attccagccg tcagcataaa
      601 aatcgatgcg gcagctgcac ccggtgcatg gaggcatgtc ccacacacgc cattgtagag
      661 cctggcgttg tagatgcccg actttgcatt tcgtacctta ccattgaaaa gaggagcccc
      721 cttacggaaa aggagtgtgg aagcctgaac ggctggtgtt ttggctgcga tatttgtcag
      781 gaggtttgcc cctggaacca aaaactactc cctataacga acgaagaagt ctcgattatc
      841 ccagagttga aacagcttaa cgcaggcaac atgatgggca tgacacaaac acagtttgaa
      901 gagtggtttg gcattacccc gttaaaaagg gctggatacg aaaagttaat gcagtcggtt
      961 agctgtgcgg tgaaagcttc acattaaaaa tgccggcagc tgccggcatt tcggtagcta
     1021 tcctagtaac ggtagtgatc ggatttgtat gggccgagag gatttacacc aatgtaatcg
     1081 gcctgtttgg gtgtaagcgt tgtgagctta accccgagct gctccaggtg taacctggca
     1141 acctcctcgt ccagctgctt gggaaggcgg taaacatcaa cagctaaatg ctttgtccat
     1201 agttcaactt gtgccaaagt ctggttggta aagctgttgc tcatcacaaa gctgggatga
     1261 cctgttgcgc agcccaggtt aaccagtcga ccctcggcaa gtatgaatat ggaatgacca
     1321 tcagggaagt agtactggtc aacctgaggc tttatgttga cttttctaac acccttttgg
     1381 gtatttaacc tatccatctg aatttcgtta tcgaagtggc caatgttgca cacaatagtc
     1441 tgatctttca tttgctgcat gtgctcaagg gtaataatat cgcagttgcc cgtagcagta
     1501 acaaagatgt taccctcggc tagtgcatcg tcaactgttt tcacctcaaa tccctccatt
     1561 gcggcttgca gggcgcatat tggatctatt tcggtaacaa ttaccctggc gccataggag
     1621 cgcatgctgc gggcgcatcc cttacccaca tcaccgtaac cgcaaaccac aactaccttt
     1681 ccggcaatca tcacgtcggt agctcgttta atgccgtcgg caagcgactc gcggcagccg
     1741 tataggttgt cgaacttgct cttggtaaca gaatcgttca cgttgatggc gggtatcagc
     1801 agctcgtttt tctccttcat ctggtaaagc cggtgaactc ccgttgtggt ttcctcggaa
     1861 actcctttta gctccgcggc aaggttgtgc catttgtttg gttcctgttg aagggtttcc
     1921 ctaatcaggt taagaataac ctcctcctca tgattttggg ctttacgctc cacgaatttt
     1981 ctatcgttct cggccttgta gccccagtgc accaaaagcg tagcatcgcc accatcatca
     2041 acaatcatgt taggaccctt accgttaggg aaagatagag cctgagcagt gcaccaccag
     2101 taatcttcga gggtttcgcc cttccatgcg aaaactggaa tacctgctgc ggctattgca
     2161 gcagcggcat gatcctgggt ggaaaatatg ttgcagctag cccagcgaac ctgagcgcca
     2221 agttcaacca aagtttcgat gagtactgct gtttgaatgg tcatgtgcag cgaaccggtt
     2281 attcttgccc cgttaagtgg cttttgggag ccatacttct gcctcagcga cattaaaccc
     2341 ggcatttctt tctctgcaat ttctatctcc ttccttcccc attcggccaa tccaatatcc
     2401 ttaaccttat agggttgcaa atttccatgc tgttccattt tgtgcttgta tttactttac
     2461 tctactttga ggggcgcaaa gttaggaata aattctgaaa aacttccgga tttagtgttt
     2521 gaagtatatt cagggcgtct attctatcaa tagccagctg ctcgctcaac tcttttatgg
     2581 agggaaaacg cttttcattc cttagccttg cgattatggc aacttctatt ccggaaaagt
     2641 atatttcgtc gtcaaaatca aagatgttta cctcgatggt ttgcttacgc atggtgtcaa
     2701 cggtaggtct gaacccgatg ttcatcatcc cgaacctgat ggagccctcg atgctgacca
     2761 ccacagcata tactccctcc ctggggatga gctttagggg atcgttgggg cgaatgttgg
     2821 ccgttggaaa gcctatttcc ctgccaactt ttttgcccga ttcaacagag ccggttatta
     2881 ggtagggata tcccagcatt tggttggccg tttcaacgtt gccagaaagc aaggcttccc
     2941 tgatttttgt tgagctaata ccttgaccat caacttgcaa cgtttcaact acttctaagg
     3001 tataggacaa ctctgcggaa agctcatgca gtaacgctgt atctcccctt ccaccttttc
     3061 caaatctatg tttggagccc accacgaaat gtttaacatg gtaatactta accaggtgcc
     3121 gaatgaagtc ctcagcctcc atgttgtaga aatcgggtgt aaattcttta atgataagaa
     3181 agtcaactcc cagctttgaa acaattttcg atttctcggt aagggtggta agaaatctta
     3241 gcttttcagg gtctttctgg agcaccagcc gggggtgtgg ccaaaaggta agaacggctg
     3301 aacctaaatt tctcaacccc gagacctgta acaccctttc tatgagggtt ctatgaccgg
     3361 catgaacgcc atcaaaaaag cccatggtta aggccacctc ctttccaagg gtttgttcgg
     3421 gcttttgaac gattatcatc gcaggtctgg ttaatacagg atgctaaggt gcaaatttat
     3481 gactttaatt acaactttac ctaagactgt attaattctg cattgaccaa cgttttaaaa
     3541 ttgttgacca gcgattattt ggatgtgggc aatggttaat ttgcaccaaa agaaaagcat
     3601 accttttggc aaggtgcaaa tatttttctg cttgtaaaga aattatcaca tgaaacatct
     3661 cttttagtga ggtgagtctt gtgaaggtta gagactgtgg ctcatttagt gttttgcctg
     3721 gaatccatca aaacttttta acccttactc atgaacaggt tgggttgatg ctaaaatatg
     3781 gtgaaaaaat cctcataaat ggagtaaaac cagcaaatag cgtagggtta tccatccatg
     3841 tccataaggc tgttgaagtc aatttctccc atctgctcga tttcgaggag ttttcccttt
     3901 tcacacttca aagtgcgggc aggaaacttt ttaatcagct ggtggttatg ggtggccatt
     3961 acaactgact ttccactttc ggcaatgcta tggagtatct gcataatccc ttcggacgat
     4021 tccatgtcca ggttacccga gggctcgtcg gccagaatta tttcggggtt gttgagaagc
     4081 gcacgggcaa tgactacccg ctgctgctca cccccagaaa gctgatgggg caacttgtag
     4141 tccttaaact ttagcccaac catttcaagc acctcggtta ttcgctgctt gatggcctgc
     4201 ttatccttcc agccggttga ccttagcaca aacagaaggt tgtcataaac gctccgatca
     4261 gatagcagct gaaaatcctg aaacacaacc ccaattttac ggcgaagctt ggggatatgc
     4321 ctgcgtttga ttgttcgaag gttgaatcca caaactgttg cctcgcccga tttaaggggg
     4381 agttcagccg ttatggtttt tattaggctg gtttttccgc tgccaaccct accgatgatg
     4441 tagagatatt cgccctgagc cagtgacagg ttaacatcgg aaagtatgag gttatcctcc
     4501 tggaagatgt tggcattgct aatttctatt accttgcttt cgtccatggt atgggttaag
     4561 cgcaaaattt gttgctaagg tataatattt atttaaacgc taggatacca catctaaggg
     4621 ttggctttgt attttttact gtttagatga aaggagtgcc atggataaag ttgtaggtag
     4681 tacttaacta cccctctacc tttggcagaa tgggggatgt gaatttttca gctacttttg
     4741 gcatttggta caaacagtta tttcggtaaa taaaaatatg gcatgagcac gagtgacaaa
     4801 aatgatatcc tgtctgttct ttatgaggct atacctgctg gcaacaagca gggagaagac
     4861 atttttacct ttggatcggt ttatgtggcg gcttcaacaa gcgaggggaa cattggtgta
     4921 tgcgccacgt tgggtgttac cgtaccctgc tttaaccctg cctattttaa tattgaccgg
     4981 gtagccgacc gggtagcgct gaacgcactg attaacgcca gggtaaacta ttcggtttta
     5041 tatgatggcg ctgcagatat cttcgatgtt gtagatttta caaggtacca aagcattgtg
     5101 atgattgggt atttcaggtc gctggttgac aagctcagca gcagcggggt cgaaacattt
     5161 attttcgacc tgaaccaaca cgatgtacct gtaatgccaa taaagctgca gcaggagtac
     5221 ctgggtaggg ccgattgcgt gatagtcagc tcaaccagca tttctaacgg aacttttaac
     5281 ggcctcatcg tcaatacgcc agcaagcacc aatgtttaca tgctggggcc ttcaacgccc
     5341 atgtgcgatg taatgttcag ctaccctcag gttaaggggc tttttgggtc ggtttttgag
     5401 ccccatgacc aaaaaaccat tcaaattatt gctgccggag gcggcactcg ccagttcaaa
     5461 acctgcatga ggaaggttta caggttgagg catgagtaag ccagccagat tattttacgg
     5521 aaataaatta aatttgccta aatgtttatg cgatggagga ggagtatatc tcaataaaag
     5581 aggctagtga atgggcgtca gaacacctag ggaaaaaagt taccacttca aacatttcct
     5641 atctaattca atatggtaga attgctaaaa ttggtgataa tggagcagcc aggatttcaa
     5701 aaaaggattt agctgattac tataactctt ttaatggtaa acgccaaatt tcatggaaaa
     5761 gtgagttagg cgatgattta aactgggcac tttcattcga tcagtataaa gaagccgaaa
     5821 caacaaagca cgtgcatagg ttacatccgt ataaagggaa atttataccc caacttgttg
     5881 agtactttat tgacagtcat acggatagct tcaaaaggga agtttttttt aaaaagggag
     5941 atattattct cgacccattt tctgggagcg gaaccacaat ggtagagtgt aatgagttgg
     6001 gaatgcatgc cattggtata gatatttctg aatttaattc acttatagct aactgtaagg
     6061 taaccaagta taatttgccc gatgttcaaa ttgaaataaa cagaattaca agtgttttaa
     6121 aggggtttct acaaaattcc aaaacaattg agtttgaaga aaaattactg gaagcactaa
     6181 aggattttaa caacctgtat tttccagttc cagaatttaa gttgaagtta agaaaaggag
     6241 aagtagatga gcaaaagtat ggaaaggaaa aggaagttga gtttatgcct tattacgaaa
     6301 agctagtaaa agattatgga attatattgc gacaggataa ggaggacact tttcttgata
     6361 agtggttttc ccagcacatt agaaatgaaa tagaacttgt ttttaatgaa attaaaaaaa
     6421 ttcaaaacag ggacacaaaa aaaataattt ccataatact tagcagaaca atgcgtagtt
     6481 gtagggccac aacacatgcc gatttggcaa cattgctgga gccagtaact acaacatact
     6541 actgtgcaaa gcacggtaaa atatgtaaac cacttttctc cattcttaaa tggtggcaaa
     6601 cctataccaa ggatactgtt aagcgtttgg tggagtttga caagctaagg accccaacca
     6661 ttcagacatg ccttacaggc gatagtaggt caatagatat tactgaggag ttgagcaaaa
     6721 agaataggga ctttgcccat caggttgcca ctcaaaaaat tagtggaatt ttttcatcac
     6781 ccccctacgt gggattaatt gattaccatg agcagcatgc ctatgcatac gatttgtttg
     6841 gttttaagcg tagagacgag ttggaaattg gtccattgta taaaggccaa ggcaaagatg
     6901 ccaaggataa ctacgttagt ggaatttcgg aagttctcaa caatgcaaaa cgattcatgg
     6961 ttgatgactt taacgttttc ctggttgcca atgacaagta taacctctac ccacaaattg
     7021 cacaaaacgc aggaatgaaa attgtaaaca cctataaacg tccagtttta aatagaactg
     7081 aaaaggataa gaacccatac tctgaaacaa tttttcactt aaaaaaaata taatggccat
     7141 caataatcag aaaaaagaag aaattaagaa tattattaaa gaaagcatcc gtagtaaatt
     7201 taagcactac aaacccgaaa ccctaagtat gccctttcat tacaggctat taggtaagga
     7261 tagaatggcg ctttactctt ttatccaatc actcaacaca actttcggaa cctcaatttt
     7321 tgaaccggtt gctgttgctt tagctcagga taaatttact aaggttcgtg cacaatactc
     7381 tgttggaaac actattagcc agggttgtca gcaggcaatc caaaccataa tgaatagcct
     7441 gtcaacgggg aaagccccat gcaaaccaaa ggagcttgaa gccataagga atgctgcttt
     7501 ggctggtaat cttgtaaaaa ttaagactgt aaaggttgac ttattccttg aggaccgaaa
     7561 cggagaacaa tacctattcg acctaaaaac tgctaagccg aatatcagta attttaaaga
     7621 ttttaaaaga actcttttgg aatgggctgg cattatctat actagcaata gcaatgcaga
     7681 tgtaaacacg ctaattgcaa ttccctacaa cccatacgag cctaaaccct acgagcgatg
     7741 gactattaag ggcatgctcg acgataagta cgagttaatg gtaggtgagg aattttggaa
     7801 ctttctcgga ggagatgacg cttataacga cctattagat tgctttgaaa ttgctggaat
     7861 tgaattaaga ccagagattg atgggtattt ttccaatttt accagacaat aacaatttgg
     7921 atgcgccatg cactcttcca cagtaattgc ttggttgaaa ctgaacccaa cttacgattt
     7981 tcaaagcaaa ttttaatact taaatttagg gatattctcg ggcatgctac ttaggtattt
     8041 cggataattg acccccttgc tccatgaaaa ggaatggaag ctcaaccagc atttctaacg
     8101 gaacttttaa cggcctcatc gtcaatacgc cag
//
LOCUS       DAOSHM010000082         8095 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_7627_length_8095_cov_23.155100, whole genome shotgun sequence.
ACCESSION   DAOSHM010000082 DAOSHM010000000
VERSION     DAOSHM010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 8095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8095
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7627_length_8095_cov_23.155100"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="PK839_09605"
     CDS             <1..192
                     /locus_tag="PK839_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010800532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOG73031.1"
                     /translation="KIFDQFYRVPSGNIHNVKGFGLGLSYVKKIVEAHGGRIWVESTP
                     GEGSLFSIFLPWNGPSEKK"
     gene            220..912
                     /locus_tag="PK839_09610"
     CDS             220..912
                     /locus_tag="PK839_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOG73032.1"
                     /translation="MEKRTIRVLLAEDDENLGFLLKEYLQAKEYDVDLYKDGEKAYRG
                     FQNNYYDICILDVMMPIKDGFTLAKEIRMANPAMPILFLTAKSLKEDVIEGFSIGADD
                     YMTKPFSIEELLMRIEAILRRTRKDSPDSEQSSFQIGRYLFDATKQTLQLDDEVRKLT
                     TKESELLKYLCINKNSLLDRNFALKTIWVDDSYFNARSMDVYITKLRKYLADDPTIEI
                     INVRGKGFKLIF"
     gene            1036..1416
                     /locus_tag="PK839_09615"
     CDS             1036..1416
                     /locus_tag="PK839_09615"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HOG73033.1"
                     /translation="MDKIIIEPTLETPRVVLDRDSSIIEFSGNSLPEDVNTFYAPILE
                     WIDNYVDNPNQQTEVSMNFDYYNTSSSKMILRILERFRDIHRKGYPVVVNWLYMEDDE
                     DMVEAGEDFADHLKIPFKFIPIQH"
     gene            1440..2912
                     /locus_tag="PK839_09620"
     CDS             1440..2912
                     /locus_tag="PK839_09620"
                     /inference="COORDINATES: protein motif:HMM:NF012925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycosylhydrolase"
                     /protein_id="HOG73034.1"
                     /translation="MATPSKLKVFSNRLFGRFKSTEEVESNRKALEVEYYEWVEFAQS
                     DDYKRFEELKSWEATKEYLTVKKEVEAIKYSESPEQREEQELKKLSRSHSIRNFLKIA
                     KSETPDFYQKMEHSQLVKELKELQQFIATPDYQAKKNQYRKDNSAEYQKELRLKELLS
                     NPDIKKYLKLEKENDLKEYFQTENSEQLKRYNELSEKVSTEDFKNRKAYLLSKNKFEQ
                     TDAYKNLQEFKQLESSEKIKWFFRLNRSNKFDDLKAWNLAFYDDFDSKNIDPHKWLTK
                     FFWGEALVGKPYSFTTDNQNYTEKGNIEIGNSTLKIITRKEASEGLAWDKKFGFTPKA
                     FAYTSGIINTGHSFRLKEGRIEAKVRMSSVQGISHAFYLVGNEMLPEVDVFLKNNAKP
                     SGISAAYFWPNGKSKVAKSVSSIGGLKLGENFYIFGVEWDSNNIVWTINGIPYKVEPN
                     RHPGISYYMVFTSAVKGSVDDSLLPATLELDWVKCWEKKE"
     gene            complement(2936..3691)
                     /locus_tag="PK839_09625"
     CDS             complement(2936..3691)
                     /locus_tag="PK839_09625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG73035.1"
                     /translation="MNSHSALRRLGYTSDQAAIMRRYISEQQGWIAHLENSRQIIIES
                     LLRQKAKTVRILGSGNLLDVPVDEILQHGSTLVLTDIIHPPQVVNRFYGASSVSFETV
                     DLTGGLVDFIAQRHARKLTFFELENFIRNITLPTFDEDLVVSLNLLSQLNDIPIAYLR
                     HKGKLSNEQLHELSEQIQQRHVDQLPVGRTLLVSDYEEEYYDEDGNLVGANPTVFVRI
                     PGCTIREWSWQFDTQMTYHEDFKTVLRVTARQW"
     gene            complement(3704..5491)
                     /gene="lepA"
                     /locus_tag="PK839_09630"
     CDS             complement(3704..5491)
                     /gene="lepA"
                     /locus_tag="PK839_09630"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005806468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HOG73036.1"
                     /translation="MEKIRNFCIIAHIDHGKSTLADRLLELTHTLSEREQQDQVLDSM
                     DLEREKGITIKSHAIQMVYPYGNETYTLNLIDTPGHVDFSYEVSRAIASCEGALLVVD
                     ATQGIQAQTISNLYLAINHDLEIIPVINKIDMDSAMVEDVKDQIVDLIGCKRDEILAV
                     SAKTGFGVDAVLEAIVNRIPPPKGDPEAPLQALVFDSMFNSFRGIIAYFKVVNGTIRT
                     GDRVKFVNTGFEYTADEVGILQLKLQPRNQISAGDVGYIISGIKESSEVKVGDTITHV
                     DSPCDKAIAGFEDVKPMVFAGLYPVDADEYEDLRSSLEKLKLNDASLTFEPESSLALG
                     FGFRCGFLGLLHMEIVQERLYREFDMDVITTVPNVSFRVHTTKGEIVEVHNPSGLPGP
                     TVIDYIEEPIINAQVITKSEYLGTVIKLCIDRRGVLKNQVFIASDRVELTFRLPLSEI
                     VFDFYDKLKSISRGYASFDYFLDGYQEAKLVKLDILLNGEMVDALSSLIHIDHAYDFG
                     RKMCEKLKDLIPRQQFDIAIQAAIGAKIIARETVKAVRKDVTAKCYGGDITRKRKLLE
                     KQKKGKKRMRQIGSVEVPQEAFLAVLKMD"
     gene            complement(5627..6424)
                     /gene="kdsB"
                     /locus_tag="PK839_09635"
     CDS             complement(5627..6424)
                     /gene="kdsB"
                     /locus_tag="PK839_09635"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015531050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HOG73037.1"
                     /translation="MKTLGLIPARYASTRFPGKPLAIIAGKPMVQWVYERASGVFDHC
                     YVATDDQRIVNVVQGFGGQSVITSEHHRSGTDRCAEAMEILQNSLNISFDVVVNVQGD
                     EPFIQPEQLQRITECFSDRTVQIATLVKAFGSNEDIFNSNAPKVVVSTEGNAIYFSRS
                     AIPFVRGKAQAEWQLAHRFFKHIGLYAYRPDILRQITRLPQSSLELAESLEQLRWIEN
                     GFTIRVLETDQETLAVDTPDDLNRVKEFAMKLLNQPDKRDTKQQPSA"
     gene            6944..7852
                     /locus_tag="PK839_09640"
     CDS             6944..7852
                     /locus_tag="PK839_09640"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOG73038.1"
                     /translation="MSHQNESVKSILLALLANLGIAITKTIAAVVTKSGAMMAESIHS
                     FADCANQGLLFWGLKASRKKPNPEHPLGYGKEIYFWSFIVALILFSMGGLFSVYEGIH
                     KVSAHEGLRNTNVAIIVLVVSMVLESISLYGCLKQIRKVRHGESLWKWYKTSRQSELV
                     VVLSEDIAALLGLTLALVAVILSIATGNPIYDAAGSIGIGILLVIVSCFLAVKVKGLL
                     IGQSVDDETSNKIRKIIESRPEVEKIFNLITIQLGPQVMVAVKAKMKATDNINQLISN
                     INACEEALTQEISTIQWVFFEPDLKD"
BASE COUNT         2396 a         1863 c         1791 g         2045 t
ORIGIN      
        1 aaaatattcg atcagtttta ccgcgtacct agtggtaaca ttcacaacgt gaagggcttt
       61 ggacttggac taagctatgt gaaaaaaatt gttgaggcac atgggggtcg aatttgggtt
      121 gaaagcaccc ctggcgaggg tagcctgttc tcaatatttt tgccatggaa tggcccttca
      181 gaaaaaaagt aaaaccataa accaaaatcc attacaatca tggaaaaaag aacaattcga
      241 gtgctgctgg ccgaagatga tgaaaacctg gggtttctgc ttaaagagta cctccaggcc
      301 aaggaatacg atgtagatct ttacaaggat ggcgaaaaag cctaccgggg ttttcagaat
      361 aactactacg atatctgcat acttgacgta atgatgccca ttaaggatgg atttacactg
      421 gccaaagaaa ttaggatggc taatcccgcc atgcccatcc ttttccttac ggccaaatcg
      481 ctcaaagaag atgtaattga aggattctca ataggtgccg acgactacat gaccaagccc
      541 ttcagcattg aagagctgct gatgcgtatc gaagccatac tccgccgcac ccgtaaggat
      601 tcgcccgaca gcgagcaaag ctccttccag attggccgct acctattcga cgctaccaag
      661 caaaccctac agctcgacga cgaggtacgc aagcttacca ccaaggagag cgagctgcta
      721 aaataccttt gcatcaacaa aaactcgctg ctcgaccgta attttgcgct gaaaaccatc
      781 tgggtggacg acagctactt taacgcccgc agcatggatg tgtacatcac aaaactccga
      841 aaatacctgg ccgatgatcc aaccatcgaa attatcaacg ttagaggaaa ggggttcaag
      901 cttatttttt agcctgggta ctgcaaccaa aaatctattt acgcatatat ttgtagcgta
      961 aaatgctttt ttggctgttt tttgcattgt aaaaaaaata ttcttacctt taggtagctg
     1021 aaattgaatg ccggtatgga taaaattatt attgaaccca cccttgaaac acctcgtgtt
     1081 gtgctggaca gagacagctc cataattgag ttttcaggaa attctctacc ggaggatgtt
     1141 aacacttttt acgctcctat tcttgaatgg atcgacaact acgttgacaa tcccaaccag
     1201 caaaccgagg tcagcatgaa ctttgactac tacaacacct cctcgtcaaa aatgatactc
     1261 cgaatactcg aaagatttcg cgacatccac cgtaaaggct atcccgtggt tgtcaactgg
     1321 ctttacatgg aggatgatga ggatatggtg gaagccggtg aggattttgc cgaccacctg
     1381 aaaatcccct tcaagtttat cccaattcag cactagcagc taaaccaaaa accgactcaa
     1441 tggctacccc gtcaaaatta aaagtattct ccaaccgcct ttttggcagg ttcaagagca
     1501 cagaagaggt tgaatcgaat cgcaaggcgc ttgaggtcga atactacgaa tgggttgagt
     1561 ttgcccaatc ggatgattat aaacgctttg aagagcttaa aagttgggaa gcaaccaagg
     1621 agtacctcac cgtaaaaaag gaggttgagg ccattaaata ctccgaatcg cctgaacaaa
     1681 gggaggagca ggagctaaaa aaactttccc gcagccactc catcagaaac ttcctcaaaa
     1741 tagccaaatc cgaaaccccc gatttttacc agaaaatgga gcattcccag ctggtaaagg
     1801 aactaaagga attgcagcaa ttcattgcaa ctcctgatta tcaagccaaa aaaaatcagt
     1861 atagaaaaga taactcagca gagtaccaga aggagcttag actcaaggag ttgctaagta
     1921 atcccgatat taaaaagtat ttaaaacttg aaaaggagaa tgatttaaaa gagtactttc
     1981 aaactgaaaa ttccgaacaa ctcaagcggt acaacgagct atccgaaaag gttagcaccg
     2041 aagattttaa aaaccgtaag gcctacctgc ttagtaagaa taagtttgag caaaccgatg
     2101 cctacaagaa ccttcaggaa tttaagcagc tggagtcgtc ggaaaaaata aaatggttct
     2161 tccggctgaa ccgcagcaat aaatttgatg acctgaaagc atggaaccta gctttttacg
     2221 atgattttga ctcaaaaaat attgaccccc acaagtggct cacgaagttc ttttggggcg
     2281 aagcccttgt tggcaaaccg tactctttta ctaccgacaa ccagaactac accgaaaagg
     2341 gaaatattga aattggcaac agcaccctaa agattattac ccgcaaggag gccagcgagg
     2401 ggctggcatg ggataaaaag tttggattta ctccaaaagc atttgcctat acctcgggta
     2461 taatcaacac cggccactct ttcaggctta aggaaggaag aattgaggca aaggttcgta
     2521 tgagcagtgt gcagggtatt agccacgcct tttacctggt gggcaacgag atgcttccag
     2581 aggtggatgt attcctgaaa aataacgcta agccgagtgg catttcagca gcttacttct
     2641 ggcctaacgg caagagcaaa gtagccaaat cggtatcctc cattggtggg ttaaagctag
     2701 gcgagaactt ctacatcttt ggtgttgaat gggattcgaa caacattgta tggaccatca
     2761 acggcatccc gtataaggtt gagcccaacc gccaccccgg tatctcctac tacatggtat
     2821 ttacttctgc agttaaagga agcgttgacg actccctgct tcccgccacc cttgaactcg
     2881 actgggtaaa gtgctgggag aaaaaggaat aacgttaaac tctctacgca aatagctacc
     2941 actgcctagc cgttacccga agcacggttt taaagtcctc atggtaggtc atctgggtat
     3001 caaactgcca gctccactcc ctgatggtgc aaccgggtat tctgacaaaa acagttggat
     3061 tagcgccaac caggtttcca tcttcatcgt agtactcctc ctcgtagtcg gaaaccagca
     3121 acgtgcgccc cactggaagc tggtcaacat gcctttgctg aatctgttcc gaaagctcgt
     3181 ggagctgctc gttactcagc ttccccttat gcctgagata ggctatagga atatcgttta
     3241 gctggctgag aaggttgagc gaaacaacca aatcctcatc aaacgtgggc agggtgatgt
     3301 tcctaataaa gttctcgagt tcaaaaaatg tcagctttcg ggcatggcgt tgggctataa
     3361 aatcgaccag gccaccggta aggtcaaccg tttcgaaaga tacggagcta gcaccgtaaa
     3421 acctattaac tacctggggt gggtggataa tatcggtaag aacgagcgtg ctgccatgtt
     3481 gtagaatctc atcaacagga acatcgagca ggttgccgct tccgagtatc ctcacagttt
     3541 tggctttttg ccgcagcaaa ctttcaatga ttatctgcct ggaattttcc agatgagcta
     3601 tccagccctg ctgctcacta atgtaacgcc gcatgatggc agcctggtcg gatgtatatc
     3661 caagccttct aagtgcagag tggctgttca ccgcaggaat agtttagtcc attttaagaa
     3721 cagccagaaa agcctcctgt ggaacctcaa cggacccaat ttggcgcatc cgttttttac
     3781 ccttcttttg cttttcaagc agcttacgct tgcgggtaat atcgcctccg tagcattttg
     3841 ccgtaacgtc cttccgaacg gccttaactg tttcgcgggc aataatcttg gctccaatgg
     3901 cagcttggat ggcaatgtcg aactgctggc gtggtatcaa gtccttgagt ttttcgcaca
     3961 ttttacggcc aaaatcgtag gcatggtcaa tgtgaataag tgatgatagc gcatcaacca
     4021 tctcgccgtt gagcaggatg tcgagcttaa ccagcttggc ttcctggtat ccatccagaa
     4081 aatagtcgaa ggaggcgtaa cctcgggaaa tggatttaag cttatcgtag aagtcgaaaa
     4141 caatctcgct gaggggcaac cggaatgtga gctccaccct atccgaggca atgaagactt
     4201 gattcttcag caccccacgc cggtcaatgc aaagtttaat gacggttcca agatactccg
     4261 acttggtaat aacctgagca tttataatcg gctcttcaat gtaatctata acggttggac
     4321 caggtaatcc gctgggatta tgcacctcaa ctatttctcc cttggtggtg tgaacacgaa
     4381 acgaaacgtt gggcacggta gtaataacat ccatgtcgaa ttcacggtac agcctctcct
     4441 gaacaatttc catgtgaagc agcccaagaa atccacaacg gaatccgaag cccagtgcaa
     4501 gcgatgactc cggctcaaag gtgagagagg cgtcgtttag cttcagcttt tcgagcgatg
     4561 agcgcaagtc ctcatactca tcggcatcaa ccgggtagag accagcaaaa accattggct
     4621 taacatcctc gaatcctgca attgccttgt cgcatggcga gtcaacatgg gtaatggtat
     4681 cgccaacctt cacctccgac gactccttga tacccgatat gatgtagccc acatcgcccg
     4741 cgctgatttg gtttcggggt tgaagcttca gctgaagaat tcccacctcg tcggcggtgt
     4801 attcaaagcc ggtatttacg aactttaccc tatcacccgt tcgaatggta ccgttaacca
     4861 ccttgaagta ggcaataatt cccctgaagg aattgaacat agagtcgaaa actaaggcct
     4921 gaaggggggc ctcaggatcg cccttgggag gaggaatacg gtttacaata gcctccagta
     4981 ccgcatcgac tccaaaacca gttttggcgc taaccgccaa aatttcatca cgcttacaac
     5041 ctatcaagtc aacaatttga tctttaacat cctccaccat agccgaatcc atatcaatct
     5101 tgttgatgac cggaataatc tccaggtcat ggttaatggc caggtacagg ttggagatgg
     5161 tttgggcctg aataccctgc gtggcatcaa caaccagcaa tgcgccctcg cacgaggcaa
     5221 tggcacgcga cacctcgtat gagaaatcca catgaccagg ggtatcaatc aggttgaggg
     5281 tgtatgtttc attcccgtaa ggataaacca tctgaatggc atggctttta atggttattc
     5341 ccttttccct ttcaaggtcc atgctatcca acacctgatc ctgctgctcg cgctccgaca
     5401 gggtgtgagt tagctcgagc aacctgtcgg ccagggtact ttttccatgg tcaatatggg
     5461 ctatgatgca aaagtttctt attttctcca tgcacattta ctgttgaagc gcaaaggtat
     5521 tcaaaaaaaa tggatatgcc tatgagccac cgctatgatg gcaacaaatc aaaaaataca
     5581 ttacctaatg cttctccagc aaaccaactt gcatggtggg atgcaatcag gccgatggct
     5641 gctgcttcgt atccctttta tccggctggt ttagcaattt catggcgaac tccttaaccc
     5701 ggtttaaatc atcgggagta tcaacggcga gcgtttcctg gtcggtttcc agcacacgaa
     5761 tggtaaatcc gttctcaatc catcgtagct gttcgagcga ttcggccaac tccagcgacg
     5821 attggggcaa gcgggttatt tgcctgagga tatcgggtcg gtaggcgtaa agcccaatat
     5881 gcttaaagaa cctatgggcc agctgccatt cggcctgagc cttccccctg acgaagggga
     5941 tagccgaacg gctgaagtat atggcattgc cctctgtgct aacaactacc ttaggagcgt
     6001 ttgaattaaa aatatcttca ttactaccaa aggccttaac cagcgtggca atttgaaccg
     6061 ttctgtcgct aaagcactct gtaattctct gcagctgctc cggttgaata aaaggctcgt
     6121 cgccctgaac attgacaact acatcaaacg aaatatttag gctattctgg agtatttcca
     6181 ttgcttcggc acacctgtcg gtcccgctgc gatggtgttc ggatgttata accgattgtc
     6241 cgccaaaacc ctgaacaaca ttaacaattc tttgatcatc ggtagccacg taacaatggt
     6301 caaaaacacc tgatgctctt tcgtaaaccc attgcaccat tggcttacct gcaataattg
     6361 ccagaggctt acccggaaac cgggtggagg catacctggc aggtattagt cctaaagttt
     6421 tcattcactc gggagtaaaa ttttacagaa caaataatgt aatgttatcc ggccgattaa
     6481 attaccgcaa cacattttaa atgtagcttt accattatgc ttacaatggc cctcgccggt
     6541 taaccagcga aaaggttcaa caaaaataag taaaaacaaa gcacatacat actgcaacag
     6601 ctcttatgct cagaaactcc tgttgaagca gtcacataat acaagtaata caagtataaa
     6661 tgtatacctg agttttagca ttaagtcaaa agaattgctc taccaaaact ggcagcatcg
     6721 gaataaaaac attcaatgcg tggctattcc ggagtaacct ggtaagtaag aaaaataatc
     6781 cgatatgccg aattataggg aatataagaa ctcctgtaaa cttttagaac atagatgtat
     6841 aggcaataaa tgtttcagcc aaaaagttta tcattctaaa aagtctaaaa aatagctcgg
     6901 gttactccat tttttgctaa ctatgcggca aaattcaggc aagatgtccc atcaaaacga
     6961 gtcggttaaa tctattctgc tagccctttt ggcgaacctg ggaattgcaa ttaccaaaac
     7021 gattgcggca gtagtaacca aatcgggtgc gatgatggcg gaatccattc actcattcgc
     7081 cgattgtgcc aaccaggggc tgctattctg gggtttaaag gcatccagaa aaaaacccaa
     7141 tcccgaacat cctttaggat acggaaagga aatatacttc tggtcgttta tcgttgccct
     7201 gatactgttc agcatgggag gactcttttc ggtttacgag ggcattcata aggttagcgc
     7261 acacgaaggg ctaaggaata ccaatgtggc tataatcgtt ctcgtggtaa gcatggtttt
     7321 ggaatcaatc tccctatacg gttgcttaaa gcaaatacgt aaggttagac atggagaatc
     7381 gttgtggaaa tggtataaaa ctagccgcca gagcgagctg gtagttgttc taagcgaaga
     7441 tattgcagcc cttctagggc ttaccctggc gctcgttgcg gtaatactat ctattgcaac
     7501 cggcaacccc atttatgatg cagcaggtag cattggtata ggcattttgc ttgtgatagt
     7561 ttcctgcttt ttggcagtta aggtaaaagg tttactgatt gggcaaagcg tggatgacga
     7621 aaccagcaat aaaatcagga aaataatcga atcaagacca gaggtagaaa agatattcaa
     7681 cctgatcacc attcaacttg gaccgcaggt aatggttgct gtaaaagcaa aaatgaaagc
     7741 tacagataac ataaatcagc tcattagcaa tattaatgca tgtgaagaag cgcttacgca
     7801 ggaaatctca accattcaat gggtattctt tgaacccgac ttaaaggatt aacacttgtg
     7861 ctcacaaaaa aaggtatgcg ttagtttcta taaccgggct tggtggggct gaacagctgg
     7921 ttaaatggta gtaaacgatg tgcgtaaaat caacagggaa caaaccatcc gtgcctaaga
     7981 ggacatctca tacacttaaa atttttcaca tttatttgta aatttcaatt gggcagtata
     8041 tttttgcaag gcaattcaac agtagagaag actattattt gaaagtcttg cggaa
//
LOCUS       DAOSHM010000083         7600 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_8343_length_7600_cov_19.807290, whole genome shotgun sequence.
ACCESSION   DAOSHM010000083 DAOSHM010000000
VERSION     DAOSHM010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 7600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7600
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8343_length_7600_cov_19.807290"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            418..1104
                     /locus_tag="PK839_09645"
     CDS             418..1104
                     /locus_tag="PK839_09645"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF001186.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HOG73039.1"
                     /translation="MQPKRFALVVAAGSGLRMGSDTPKQFMELDGLPVLMRTISVFLT
                     LRDIPEVVLVLPEAQITTWQRLCKKHHFETNLQIVPGGSTRFESVKNGLELLPADDSL
                     VAIHDGVRPLVTHEVVERCYAMAARGVCAIPVVNPVESVMIASGGKFTPFPRNNVLLV
                     QTPQTFRSATIKHCYNQPYSELFTDDASVAAACGVELHTVEGNRENIKITTPADLLTA
                     RVLLQAMKTD"
     gene            1129..2301
                     /locus_tag="PK839_09650"
     CDS             1129..2301
                     /locus_tag="PK839_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG73040.1"
                     /translation="MSFVLKEVDNRKTRKDFLNLPVELYRDYPSWIRPLNNDIESVFN
                     PKTNKQFRHGEAIRWVLYSDNKPVGRIAAFIDYEIARNNEQPTGGVGFFECINSPEAA
                     TQLFDTAKEWLQQRGMEAMDGPINFGDRDRWWGLLVEGDYPPNYCMDYHPPYYRELFE
                     GYGFKPYFHQFTYYRPVNPEGLDPIIREKAERIARNPAYHVTIISKRNLKKFAEDFRT
                     IYNNAWGRYTGVKKMSHIHAMALMKTLKPIIDPELMYFAYYNNEPIGFFIMVPDLNQV
                     VRHLNGQFGLVHKLKFLYLLKVKKVCTKAIGLIFGIVSQHQGKGVEGALVTEFANRAL
                     RPDFQYKELELNWIGDFNPTMRRVASQIGGKIRKIHITYRYMFDRTKEVVPPRRVS"
     gene            2418..4412
                     /locus_tag="PK839_09655"
     CDS             2418..4412
                     /locus_tag="PK839_09655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG73041.1"
                     /translation="MLKRFLGLLLLTLSGYMVAAQRDIEPRLVFSDTSRFNFTGEWQY
                     LSTDIFLLNGDRFSNLINELNFPNKGNGKIRRKSKLEEERLEYLFITAKLKNVKFFGE
                     NDITYPLYNFQISRDKDNRYQTLVSDNIDHVRIIDNLPLYSAHDYIDAEISVKAITNN
                     DRDQMLSLVATQLQNLSKIKTPTDAVMGIIGEFGSFIEANTRKKEYRFSSTIRLFEQK
                     NFDTRVHSLKLYVLTTSNSKPIEVNTALIQNFMDTVRHGQVGRQTLKELLAYHNYPVI
                     AVVNYKSLYRMEPIGGDEVTFASIETRKLRVENDYRQGLINAETYRQEKDFIAFLSVY
                     ANFKSYLDAYNLNYRTGNSDAVSISLFKVMQYYRQLVKTFEEMRFKYRGNNTFANIFR
                     REYESVLGFASLYLDDDHNLKATKELVNSLVKLDTIPMLAAADMEAYIASLRFSGAFK
                     PDLMRQTPEGQLITNHLASLEEQLYRSTYEDEVRKLNDVEASASTRNATEKLLAMIKN
                     TSCGLCRERAIAAINSFGQRMDEFTRKQKVAQRDSLVNSLQPWVFNTIEQLQLIKSNL
                     QVLYPLDSLPESAKYLSTKVAEAERDLGNFRDLAKVDVSQMELSLVANLVEKLKSYHQ
                     MVEENVKLVCGVRPELCVRALDVNKSQPADSLKAVDSCSI"
     gene            4564..5949
                     /gene="glmM"
                     /locus_tag="PK839_09660"
     CDS             4564..5949
                     /gene="glmM"
                     /locus_tag="PK839_09660"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005677088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HOG73042.1"
                     /translation="MALIKSISGIRGTIGGNPGDNLTPLDIVKFTSAYAVWLKQRNPT
                     KRCRVVVGRDARISGEMVNNLVVGSLMGCGLDVVNLGLATTPTVEMAVIHKKTDGGVI
                     LTASHNPRHWNALKLLNEKGEFLSDADGHHVLDLAEGDTLNFAAVDDLGLVEEDSSFI
                     DEHISQILKLRYVDVDAIAGANFTVAIDCVNSVGGLAIPKLLNALGVERVIELYTEPT
                     GNFPHNPEPLPEHLGEISAVVRERKANVGFVVDPDVDRLAIVNEDGSMFGEEYTLVAC
                     ADYVLSHNPGNTVSNLSSTAALREITRRWGKEYYTSAVGEVNVVERMKEVNAVIGGEG
                     NGGVILPELHYGRDALVGIALFLSHLARSGKKCSSLRAAYPSYFISKNKINLKPGVNP
                     DELLAKLLEQLKQYPVNTTDGLRVDFDDSWVHMRKSNTEPIIRIYAEAQNPEKADELA
                     KKMMEQIVKMF"
     gene            complement(6049..7524)
                     /gene="ftsZ"
                     /locus_tag="PK839_09665"
     CDS             complement(6049..7524)
                     /gene="ftsZ"
                     /locus_tag="PK839_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOG73043.1"
                     /translation="MGEELINFDLPATTESIIKVIGVGGGGGNAVNYMHRMGIKDVDF
                     VLCNTDAQALFNSPVKMKLQLGASLTEGRGAGNKPEIGRQAAIESSQQISDLLLRNTK
                     MVFITAGMGGGTGTGAAPVIAQIAKELGILTVAIVTIPFRNEGKKRIAQAIEGIENIR
                     QHVDSLLVVNNEKILEIYGNLKVTEAFSKADDVLATAAKGIAEIITVHGYINVDFADV
                     ETVMANSGVALMGSGRAAGENRAITAVKAALESPLLNNNDISGARNILLNVTYGQDEI
                     TMEEIGLIIEYVQSAAGDDADLIWGNGCDPQLGDDLKVTVIATGFSSSSIPELYIRQK
                     KVDVLNLDGDIFANRVEKTPLVGGSRLQEANPGIPKVKRNEPEDGRQRVIEFDLSSDE
                     IQVKTIGTPKASTPEIPLPVSKTTGKPASQPQKPDSQQGKGKYGDIDELENVPAYKRK
                     NMPLDPVDHSKPDSISRLTLNEEDGDIFLRPDNSFLHDNVD"
BASE COUNT         2005 a         1902 c         1830 g         1863 t
ORIGIN      
        1 tctattcaaa attacaaaag cttttcattt gataaaccaa acttatgaac ttttttacgt
       61 ttcctcaaat gagctgctaa ccttctgtaa acatttccag ctgctgcatg catcaatcta
      121 cgtgattctt aaactcctga gcccgggtgg ctcttggatg tagcccaccc ctgttcccgc
      181 ttcaaccaaa tgcccggagc aaccagcgca gggtggcagg gttgattttc gggtttccat
      241 cgtggccttt ctaagctcgc actatgctcc cttcagcatt ccggccggct ggtcggaaat
      301 gggctcaact cttcagccca gctggcttcg cacctgtagt cttttgaacc aaaaatctcc
      361 caggctggta tgatttagtc aaaaatggct aactttcgcc atcaaattgc cataaaaatg
      421 cagcccaagc gttttgccct ggttgttgcc gctggctccg gcttacgtat gggctccgac
      481 accccaaagc agttcatgga gctggatggg ttgccggtgc tgatgcgcac tattagcgtt
      541 ttcctcaccc tgagggatat acccgaggtt gtgctggtgc ttcccgaagc tcaaatcaca
      601 acatggcaaa ggctttgcaa aaagcaccat tttgaaacca acctgcaaat tgttccgggg
      661 ggtagcaccc gctttgagtc ggtaaaaaac ggtttggagc tgctgcccgc agacgactcg
      721 ctggtggcca ttcacgacgg cgttcgtccg ctggttacac atgaggtggt tgagcggtgc
      781 tacgccatgg ctgccagggg ggtgtgcgcc attccggttg tgaatcctgt tgaatcggta
      841 atgattgcaa gcggcggcaa gtttacccca ttcccccgca acaacgtttt gctggtgcaa
      901 acaccccaga cttttcgctc agcaaccata aagcattgct acaaccagcc ttacagcgag
      961 ttgtttaccg acgatgcctc ggttgctgct gcctgcgggg ttgagctcca taccgtggag
     1021 ggaaacaggg aaaatatcaa aataaccaca ccggccgatt tgcttaccgc ccgggtcctg
     1081 ctacaagcca tgaaaaccga ttaatagcca tagctaaccg attgtgccat gagttttgta
     1141 ttgaaagagg ttgacaaccg aaaaacccgt aaagattttt taaaccttcc ggtagagctt
     1201 taccgcgatt atcccagctg gatcaggccg ctcaacaacg atattgaaag cgttttcaac
     1261 ccaaaaacca acaagcagtt caggcatggc gaagccattc gatgggtgct atactccgat
     1321 aacaagcctg ttggacggat tgccgctttt attgactacg agatagcccg caacaacgag
     1381 cagccaactg gcggggttgg atttttcgag tgcatcaaca gccccgaagc tgccacacag
     1441 ctattcgaca ccgcaaagga gtggctgcag caaagaggaa tggaggccat ggacggccct
     1501 atcaactttg gcgatcgcga ccgttggtgg gggctgctgg ttgagggcga ctacccacca
     1561 aactactgca tggactacca tcctccctac taccgcgagc tttttgaggg ctacggtttt
     1621 aagccttact ttcaccagtt cacctactac cgtccggtga atcccgaagg gcttgaccca
     1681 attatacgcg aaaaggcgga acgcatagcc cgtaaccccg cctaccatgt taccatcatt
     1741 tccaaacgca accttaaaaa gtttgccgag gatttccgta caatatacaa taacgcctgg
     1801 ggaaggtaca ccggggtaaa aaaaatgtcg cacattcatg ccatggccct gatgaaaacc
     1861 ctgaaaccaa ttatagaccc cgagctgatg tactttgcct actacaacaa tgaacccatc
     1921 gggttcttta tcatggtgcc cgatttaaac caggtggtta ggcatttgaa cgggcagttt
     1981 ggtctggttc ataagctcaa atttttatac ctgctgaagg ttaagaaggt ttgcaccaag
     2041 gccattggcc tgatttttgg aattgtgtcc caacatcagg gcaagggtgt agaaggtgct
     2101 ctggtaacag agtttgccaa cagggcacta agacctgact ttcagtacaa ggagctggag
     2161 ctgaactgga ttggcgactt taaccctacc atgcgaaggg tagccagcca gataggaggg
     2221 aaaatacgta aaatccatat cacctaccgc tacatgttcg acagaaccaa ggaggtggtc
     2281 cctccccgaa gggtgagctg agggtggaca aaagaaacag gctggttgaa cgctccgatg
     2341 aaatgcgcta tgctaaatat ttaattttga agctagctat gatgaagcag aatgctgtta
     2401 taaacccacg tatgccgatg ttaaaacgat ttctcgggtt gcttctttta acattatcgg
     2461 gctatatggt tgctgcccag cgcgatatag aacctcggct agtattttcc gacacctcca
     2521 ggttcaactt tacaggcgaa tggcagtacc tgtcaaccga tatcttcctg ctcaacggcg
     2581 acaggtttag caacctaatc aacgagctca actttcctaa taagggaaat ggcaaaatac
     2641 gccgaaaatc gaagctcgag gaggaacgcc tggagtacct gttcatcacg gcgaagctga
     2701 aaaatgtgaa atttttcggg gagaacgaca tcacctaccc actctacaac ttccagatat
     2761 cgagagacaa ggacaaccgt taccaaaccc tggtgagcga caacatcgac catgtgcgga
     2821 taatagacaa cctacccctc tactccgcac acgattacat tgatgcggag attagcgtta
     2881 aagccattac caacaacgat cgcgaccaga tgctctcgct cgttgccacc cagctgcaaa
     2941 atctatccaa aataaaaacc cctaccgatg cggtgatggg cattatagga gaattcggca
     3001 gctttattga ggctaatacc aggaaaaagg agtataggtt tagctccacc atcaggctat
     3061 ttgagcagaa aaattttgat acccgcgttc attccttgaa gctatacgtt ttaaccactt
     3121 ccaacagcaa gcccattgaa gttaacacag ctcttataca aaactttatg gacaccgtaa
     3181 ggcatggcca ggttgggcgc caaacgctga aagagctact tgcataccac aactacccgg
     3241 taatagcggt ggttaactac aagtcgctct accgcatgga gccaatcggt ggcgatgagg
     3301 taacctttgc cagcatcgaa acgcggaagc tgagggttga aaacgactat cggcaggggc
     3361 tgataaacgc cgaaacctac cggcaggaga aggatttcat cgccttcctg tccgtttacg
     3421 ccaacttcaa gagttacctc gatgcctata accttaacta ccgaaccggt aacagcgacg
     3481 ccgtttccat aagccttttc aaggtgatgc agtactaccg gcagctggtt aagacctttg
     3541 aggagatgcg atttaagtac agggggaaca acacctttgc aaatatcttt agacgagagt
     3601 atgagtctgt gctgggattt gcctcgctat acctcgacga tgaccacaac ctgaaagcaa
     3661 ccaaggagct ggtgaactcc ctggtaaaac tcgacaccat cccaatgctt gctgctgccg
     3721 acatggaggc atacattgcc tcactccgct tctcgggggc attcaagcct gaccttatga
     3781 ggcaaacacc cgagggtcag ctgatcacca accaccttgc cagccttgaa gaacaactct
     3841 acaggagcac atacgaggat gaggttagaa agctgaatga tgttgaagcc agcgctagca
     3901 cccgtaacgc caccgagaaa cttttggcga tgattaagaa tacctcatgc gggctatgcc
     3961 gtgagcgggc cattgccgca atcaactcct ttggccaacg aatggatgag ttcaccagga
     4021 aacagaaggt tgcccagcgt gatagcctgg taaacagcct tcaaccctgg gttttcaaca
     4081 ccattgaaca gctacaactt ataaaaagca acctccaggt gctttaccct ctagattccc
     4141 ttcccgaaag tgcaaagtac ctcagcacaa aagttgctga ggctgaacgc gatttgggta
     4201 atttccgtga tttggctaaa gttgacgtat cgcagatgga gctgtcctta gtggctaacc
     4261 tggttgaaaa gctaaaaagc taccaccaga tggtagagga aaacgttaag ctggtttgcg
     4321 gggttagacc agaactctgt gttagggcat tagacgtcaa taaatcccaa ccagctgatt
     4381 cgctgaaagc ggttgattcg tgctccattt aagccgatgt tgtggaagtt tgaatgcctg
     4441 cagctggagt agctaaaaat ttaaacctcc tggtgcaaat attgaggagg aaaaacctaa
     4501 catttacaac cttgaggcaa ataattgata gaaacttata aaaattaact ggagaaacta
     4561 gacatggcgc tgattaaatc tatatcggga attcggggaa ccattggggg aaatccgggc
     4621 gacaacctaa cacccttgga tattgtaaag tttacgtcgg catacgccgt atggttaaag
     4681 caacgcaatc caaccaagcg ctgcagggtt gtagttggca gggatgcacg aatttcgggc
     4741 gaaatggtca acaacctggt tgttggttcg ctaatggggt gcggtttgga tgttgttaac
     4801 cttggcctgg ccactactcc tacggttgaa atggctgtaa tccataagaa aaccgatggc
     4861 ggagtaattc taacagccag ccataacccc cgccattgga acgccctaaa gctgctgaac
     4921 gaaaaaggtg aatttctctc cgatgccgac gggcatcatg tgcttgacct tgccgaaggc
     4981 gataccctta actttgcagc agttgacgat ctggggctgg ttgaggagga tagctctttc
     5041 attgatgagc atatcagcca aatactcaag ctgcgttatg tagatgttga cgccattgcc
     5101 ggtgctaatt ttacagtggc tatagactgt gtaaattcgg ttggaggact agccattcca
     5161 aagctgttga acgcattggg tgttgaaagg gtgattgaac tctacaccga gccaaccggt
     5221 aattttccac ataaccccga acctcttcca gagcatctgg gcgaaatttc agctgttgta
     5281 cgtgagcgca aggctaatgt aggctttgtg gttgaccccg atgttgaccg gttagccatt
     5341 gttaacgagg acggaagcat gtttggtgag gagtacaccc tggtggcctg tgccgattac
     5401 gtgctcagcc ataacccggg taataccgtt tccaacctgt cgagcaccgc agcgcttcgt
     5461 gaaataaccc gtagatgggg aaaggaatac tacacttcgg cagtgggcga ggtaaatgtg
     5521 gtggagcgaa tgaaagaggt gaatgccgta attggcgggg agggtaatgg tggagttatt
     5581 ctaccggagc tgcattatgg aagggatgcc ctggtgggta ttgccctttt cctgtcgcat
     5641 cttgcccgat cgggcaaaaa atgctcctcc ctaagagcgg cctatcccag ctacttcatc
     5701 tcaaaaaata aaattaacct gaagccaggc gtcaaccccg acgagttgct ggcaaagctg
     5761 ctggaacagc taaaacaata tcccgtaaac acaaccgatg ggctgagggt tgacttcgac
     5821 gactcatggg ttcacatgcg caagtcgaac accgaaccca tcatcaggat ttacgccgaa
     5881 gcccaaaacc cggaaaaagc cgacgagctg gctaaaaaaa tgatggagca aatcgtgaag
     5941 atgttttaac catgcaacca tcagtaacca ggtagcaggg ctttggttgg caaggcagct
     6001 aaccggcaac agcgctgagc caacccgatt caacattggc gctaatttct agtcaacatt
     6061 atcgtgcagg aacgagttgt caggccgaag gaatatatcg ccatcctcct cgttaagggt
     6121 taacctggat atgctatcgg gtttagagtg gtcaaccggg tcgagtggca tgttttttcg
     6181 cttgtatgca ggcacattct ccagctcatc aatatcaccg tatttccctt tgccctgctg
     6241 tgaatcgggt ttttgaggtt ggctggcggg ttttccggtt gttttagaca ccggaagcgg
     6301 aatttcagga gtggaggcct taggcgtgcc tatcgtttta acctgaatct catccgatga
     6361 gaggtcaaac tcaataaccc gctgacggcc atcctccggc tcgtttcttt ttaccttagg
     6421 aataccagga ttagcctcct gcaaccggct gccgccaacc aaaggagttt tttcaacccg
     6481 gttggcaaat atatcgccgt caaggttaag cacatcaact tttttctggc gtatgtagag
     6541 ttccggaatg ctgctggagc tgaaaccggt agcaattacg gtaaccttta ggtcgtcgcc
     6601 cagctgtggg tcgcagccat taccccatat caaatcggca tcgtctccag cggccgattg
     6661 aacgtactca ataataagcc caatctcctc catggtgatt tcatcctgtc cgtaggtaac
     6721 attcagcaat atattacggg cacctgaaat gtcgttgttg ttaagtagtg gcgattccaa
     6781 cgcagctttt accgctgtga tagccctgtt ttcaccggcg gcccttcccg aacccataag
     6841 cgctaccccg ctgttggcca taacggtttc aacatcggca aaatcaacgt tgatgtagcc
     6901 gtgaaccgta attatctcag ctattccctt ggcagctgtt gccagcacat cgtcggcttt
     6961 tgagaaagcc tcggtaacct taagattccc gtaaatttca agaatttttt cgttgtttac
     7021 caccagcagt gagtccacgt gctgacgtat gttttctata ccctcaatag cttgagctat
     7081 gcgctttttc ccctcatttc taaaaggaat tgtaacaata gccactgtta gaatacccag
     7141 ctccttcgct atctgtgcaa ttacgggtgc agcacctgtc ccggttccgc cacccatccc
     7201 tgcagtgata aaaaccattt tggtgttgcg taaaagcaaa tcggaaattt gctgggagct
     7261 ctcaatggcg gcctgcctgc caatttcggg tttgttgccg gctccgcgtc cctcggttaa
     7321 agaggcgccc agctgaagct tcatcttaac ggggctattg aaaagtgcct gggcatcggt
     7381 gttgcatagc acgaaatcaa catcctttat ccccatgcgg tgcatgtagt ttacagcatt
     7441 tccaccgccc cctcccacgc caataacctt aattatggat tcggttgttg caggtaaatc
     7501 gaagttaatc agctcttcgc ccatggctca actatttagt aatatttata attcaaaatt
     7561 tagtccggct tttgacggaa cttgtccgcc tactggcgga
//
LOCUS       DAOSHM010000084         7351 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_8748_length_7351_cov_18.209019, whole genome shotgun sequence.
ACCESSION   DAOSHM010000084 DAOSHM010000000
VERSION     DAOSHM010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 7351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7351
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8748_length_7351_cov_18.209019"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(290..1990)
                     /gene="ftcD"
                     /locus_tag="PK839_09670"
     CDS             complement(290..1990)
                     /gene="ftcD"
                     /locus_tag="PK839_09670"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015546043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HOG73044.1"
                     /translation="MAEKKLIECVPNFSEGRDSGLIKQITDVIEGVEGVKLIDVDPGK
                     ATNRTVVTFVGTPDEVVEAAFRAVAKAKELIDMSRHHGAHPRFGATDVCPLVPVANIT
                     MDEVVEYARALAKRIGDELGIPVYCYEYAALEEKRRNLASCRAGEYEGLPKKLQDPEW
                     KPDFGPAQFLPKTGAIAVGARNFLVAYNVNLNTTSTRRANAVAFDIREKGRPMRVGNP
                     VTGKIATDENGKEIWIPGTLKACKAIGWFIEEYGIAQVSINLTDITVTPVHVAFEEAS
                     KKAQERGLRVTGSEIVGVVPLSAMLEAGKYFLRKQQRSVGIPDREIIRIAVKSMGLDE
                     LYPFDPDKKIIEYILEEKGTRQLAGMTLESFSRETASESPAPGGGSISAAMGAFGAAL
                     AAMVANLSAHKPGWDDRWEEFSAWAEKGKYYMEQLLALVDEDTRAFNRIMEAYGLPKG
                     TDLEKAARSKAIQEATIQAIEVPFRVMNLCYESLEVIRAMARQGNPSSVSDAGVGALA
                     ARSAVLGAFLNVKINSSGLKDKALVKDYLDRGNQIASKTIAMEEEILSDIEKNFFASL
                     "
     gene            complement(2153..3409)
                     /gene="hutI"
                     /locus_tag="PK839_09675"
     CDS             complement(2153..3409)
                     /gene="hutI"
                     /locus_tag="PK839_09675"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014776402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HOG73045.1"
                     /translation="MQILLLNIKKLVQVEEKPVQWVAGQNMANLKCIDNAFLLMENGL
                     IKDFGSMRDVPSFAGLPSDVLIYNVNGRMVFPSFCDSHTHLVYAGSREVEYIDKIKGL
                     SYEEIARRGGGILNSAKLLSQTSEDELYEQALPRVKEIIRKGTGAVEIKSGYGLSTEN
                     ELKMLRVIRRLSEATPLTIKATFLGAHAVPAEYKGRQAEYVDQIINEMIPQVAAEDLA
                     DYIDVFCDRGFFTVEDTERILMAGMKYGLRPKIHANELDYSGGIQVGVKYNALSVDHL
                     EFTGDDEIQTLLGSETMPTLLPGAAFFLGMDNPPVRKMIDAGLPIALASDFNPGSSPS
                     GDMKFIMSLGCIRLKMLPEEVINATTINGAYAMGLSETHGSICRGKVANIFITKPIPT
                     LEYMPYAYTSDLVETVFLNGNITNVC"
     gene            3639..4562
                     /locus_tag="PK839_09680"
     CDS             3639..4562
                     /locus_tag="PK839_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002561313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOG73046.1"
                     /translation="MKRDIITIDREKCNGCGQCVGGCHEGALQLIDGKAVLVSELMCD
                     GLGACIGECPEGAITVERREADAYDEVTTLSQMVASGKNTTVAHLKHLKEYHQTDYLR
                     QAVEWLLSNRKNVSFSVDEVMQEVHNHSGSSCGCGNGGKHEPTQQSHHGKHVDGCPGS
                     AERSFGNNIRVEASKMVSGKSELTHWPVQLHLINPHSEHFKGSNLLLAADCVAYSQGS
                     FHSQHLAGKTLAIACPKLDSNKEEYVEKITALIDDAQVDTITVMKMEVPCCGGLLQLA
                     RLARDKARRSVPIKVITVGIQGDILDSVWEN"
     gene            4702..6348
                     /gene="hcp"
                     /locus_tag="PK839_09685"
                     /gene_synonym="priS"
     CDS             4702..6348
                     /gene="hcp"
                     /locus_tag="PK839_09685"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002561314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HOG73047.1"
                     /translation="MSMFCFQCQETAKGTGCTIKGVCGKSDDLANMMDLLMFVTKGIC
                     VYSTALREQKGYENPIVNKFVFDSLFTTITNANFDKQVISNRVSSALEIRETLRNEAS
                     LQGVRVDTSFEGCTWSGTPAEYEAKAALVGVLSEKNEDIRSLKELITYGVKGMAAYTE
                     HAYNLGHEDVQNYAFMQRALASITRNLSVDELVALTLETGKFGVNAMALLDKANTSSF
                     GNPEITKVNLGVRSNPGILISGHDLKDMEELLAQTEGTGVDVYTHSEMLPANYYPAFK
                     KYRHFVGNYGSSWWHQREDFETFNGPILFTTNCIVPPLNNATYKDRIFTTGAAGYPGS
                     KHVADRKPGQQKDFSEIIALAKTCKAPTQIETGEIIGGFAHAQVFALADKVVDAVKSG
                     AIRKFVVMAGCDGRMKDRTYYTEFAQKLPKDTVILTAGCAKYRYNKLALGDINGIPRV
                     LDAGQCNDSYSLAIIALKLKEIFQLNDINELPIAYNIAWYEQKAVIVLLALLYLGVKN
                     IHLGPSLPGFLSPNVTNVLVDNFGISGITTVDEDIKLLVG"
     gene            6446..6772
                     /locus_tag="PK839_09690"
     CDS             6446..6772
                     /locus_tag="PK839_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007657820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOG73048.1"
                     /translation="MNNEFEKEKKFNFLDEVDYSTGGVVSKNVIKKPTGNISLFAFEK
                     GEGLSEHTAPFDALLQVIDGEAKVTINGKPHDLKAGESIIMPANSPHAVFAAERFKMV
                     LTMIKE"
     gene            7044..7349
                     /locus_tag="PK839_09695"
     CDS             7044..7349
                     /locus_tag="PK839_09695"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOG73049.1"
                     /translation="MNIITPENAEKVPFALNGRKMYSSPTAEIVHLTLKPGETVDRHS
                     NPFDVVFYMLEGEATIEIDGVKAILKTDCCLEVKRDRPRGMSNNTGKDVRILVFKLF"
BASE COUNT         1998 a         1799 c         1712 g         1842 t
ORIGIN      
        1 ccatcctcgg aactggtaac cgttccggta atcctcactg tttgcgcctg cgccagtgtt
       61 acacctacaa gcagcaaacc tgcaagaaaa acacacagtt ttttcataaa gcaagtttta
      121 ggtttacatt aaattaaagt ttaatttgcg tggctaaggt attaaattat gtgcctgttt
      181 ccaacaaata tttttagttt taattaacaa acaccacgtt tttttttgaa aaattagcat
      241 ttaacactta taaccatctg gttacctgca agctaagcaa actgttaaat tacagggagg
      301 caaagaaatt tttttcaata tccgagagta tctcctcctc cattgcaatc gttttcgatg
      361 caatttggtt gcccctgtcc aggtaatcct taaccaacgc cttgtccttc agcccgctgg
      421 agttgatttt tacattcagg aatgctccca gcacagccga tcgggcagcc agcgccccta
      481 caccggcatc ggaaacggag cttggattgc cctgccgggc catagccctg attacctcca
      541 gcgattcgta gcaaaggttc atcaccctaa agggaacctc tatggcctga atggttgctt
      601 cctgaattgc cttgctgcgt gcggccttct cgaggtcggt tcccttggga agcccgtagg
      661 cctccattat ccggttaaag gctcgggtat cctcgtcaac tagcgctagc agctgctcca
      721 tgtagtattt tcccttttct gcccaagctg aaaattcctc ccaccggtcg tcccatccgg
      781 gtttgtgggc cgaaaggttt gccaccatgg cggccagcgc tgccccaaaa gcgcccatgg
      841 ccgccgaaat ggaaccaccg ccgggcgcgg gcgattcgga ggctgtttcg cggctaaaac
      901 tttcaagtgt cattcctgca agctgccttg tgcccttttc ctcgagaatg tactcgataa
      961 tctttttatc ggggtcgaac gggtagagct catcaagccc catggactta acggcaatcc
     1021 ggatgatttc cctgtcgggg atgcccaccg atcgctgctg cttacgaagg aagtacttcc
     1081 cggcctccag catggcgctc aggggcacaa cgcccacaat ctcggagccg gtaacccgta
     1141 atccacgttc ctgagccttt ttacttgcct cctcaaaagc cacatgcacc ggcgtaaccg
     1201 ttatgtcagt taggttgatg gatacctgtg caatgccgta ctcctcaatg aaccagccga
     1261 ttgccttgca cgcctttagc gttccgggaa tccatatctc tttcccattc tcatcggtgg
     1321 caattttacc ggtaacgggg ttcccaaccc gcatgggacg acccttctca cgaatatcga
     1381 atgccacggc attggccctt cgggttgagg tggtgttaag gttgacgttg taggccacca
     1441 ggaaattcct tgcaccaaca gcaatagcgc ctgttttcgg gagaaattgc gcagggccga
     1501 agtcgggttt ccactccgga tcctgcagct ttttaggaag tccctcatac tctcccgccc
     1561 tgcagctggc caggttgcga cgtttctcct caagcgcagc atactcatag cagtaaaccg
     1621 gaatgcccag ctcgtcgcca atccttttgg ccagcgcccg ggcatactcc accacctcat
     1681 ccatggtgat gttggccaca ggtactagcg ggcaaacatc ggtggcgcca aaccggggat
     1741 gtgcgccgtg gtgcctgctc atgtcaataa gctcctttgc tttggcaact gcccggaatg
     1801 ccgcttcaac cacctcatcg ggggttccaa caaaggttac aaccgtacgg ttggttgcct
     1861 tgccagggtc aacatctata agcttaacac cttcaacacc ttctattaca tcggttatct
     1921 gtttaattaa accactatcg cgcccctcag aaaaatttgg aacgcactca attagctttt
     1981 tctcggccat ttcagtatga ataaattaga tgtatgataa ataaaataaa ggataaagga
     2041 taaaggataa aggataaaaa acaaaactca aatcattcat ttttcttctc tttaacccca
     2101 agacagtctc aggacaaact tttaactttt tttcggttcg ttgttcacgg tttcagcaga
     2161 cattagtaat gttgccgttg agaaaaaccg tttcaaccag gtcggaggtg tacgcatagg
     2221 gcatgtactc aagagtaggt attggttttg taataaagat attggctacc ttgcccctgc
     2281 aaatgctccc atgagtctcg gaaagcccca tggcatatgc accgttgatg gttgtagcgt
     2341 tgattacctc ctcaggaagc atttttagcc taatgcaacc caacgacatg atgaacttca
     2401 tgtcgcccga aggcgaagaa ccggggttga aatcggatgc aagggcaatt ggaagccctg
     2461 catcaatcat tttacgcacg ggcgggttgt ccattccaag aaaaaatgct gcccctggaa
     2521 gcagggttgg catggtttcg gacccaagca gggtttgaat ttcatcatca cctgtaaatt
     2581 ccagatggtc aaccgacaag gcattgtact ttacgccaac ctgtatgcca ccagagtaat
     2641 cgagctcatt ggcatgaatt tttggtctca ggccgtactt catgccggcc atcaggatgc
     2701 gctcggtatc ctcgacggta aaaaatcccc tgtcgcagaa tacgtcaata tagtcggcca
     2761 ggtcctcggc tgctacctgg ggtatcatct cgttgataat ctggtcaaca tattcggctt
     2821 gccggccttt atactcagca ggaactgcgt gggctcctaa aaaagtggcc ttaatggtta
     2881 gcggtgttgc ttcactaagc ctgcggataa ccctcaacat tttcagctca ttttcggtgc
     2941 tcaggccgta gccgctttta atttcaacag ctcctgttcc cttgcggata atttctttaa
     3001 ccctaggcaa tgcctgctcg tacagctcgt cctcggaagt ttgggagagt agctttgcgg
     3061 agttaagaat tcccccgcca cgcctggcaa tttcctcgta cgatagccct tttatcttat
     3121 caatatactc aacttcgcgg ctgccggcat ataccagatg ggtgtgagag tcgcagaagg
     3181 agggaaaaac catccgccca tttacgttgt aaatcagcac atcgctgggt agcccggcaa
     3241 atgaaggaac atccctcata gacccaaaat ccttgattaa accattctcc atcagcagga
     3301 atgcgttatc aatacacttc aggttggcca tattttggcc cgcaacccac tgaacaggct
     3361 tttcctcgac ctgtaccaac ttttttatgt taagtagcag tatttgcatg gttttcagca
     3421 taaatctccg acgaaggtag caataaaatt gggaagaact aaatattatt taataaaagg
     3481 tatgcgttaa agccctgcac cagccagcat cctagtgttt ttgagctggt attaaaaatt
     3541 ttactcgttt tgtaacattt gttacataat taggaggggg tggtgcctta gctttgctcc
     3601 agaaaaaggt tgaaattttt tttcgtaaaa aagcaggcat gaaaagagat atcattacaa
     3661 ttgacaggga aaaatgtaac ggttgcggcc aatgtgtagg tggatgccac gagggtgccc
     3721 tgcagctgat tgacggcaag gcagttttag ttagcgagct gatgtgcgat gggctgggag
     3781 cctgcattgg tgaatgcccc gagggagcca tcaccgttga gcggcgcgag gccgatgcct
     3841 acgatgaggt aaccaccctc agccaaatgg tagccagcgg caaaaatacc acagttgccc
     3901 atctaaagca ccttaaagaa taccaccaaa ccgactattt acgacaagct gtagagtggc
     3961 tgctttcaaa tcgtaaaaat gtttccttta gtgttgatga ggtgatgcag gaggttcata
     4021 accactccgg cagcagctgt ggttgcggaa atggagggaa acatgaaccg acacaacagt
     4081 cccaccatgg taaacatgtg gatggatgcc ccggttcagc agaaaggagt tttggaaata
     4141 atatacgtgt tgaggcatca aaaatggtat ccggcaagtc ggagctaacc cattggccgg
     4201 ttcagctcca tctgataaat ccgcattcgg aacactttaa ggggtcgaac ctgctgctcg
     4261 cagccgattg tgtagcctac tcgcagggga gcttccacag ccagcatctt gccggaaaaa
     4321 cgctggccat tgcatgtcct aagcttgact ccaacaagga ggagtacgtt gagaagatta
     4381 ccgcgcttat tgacgatgcc caagtggaca ccatcacagt gatgaagatg gaggttccct
     4441 gctgcggcgg gttgctccag cttgcccggc ttgcccgcga taaggccagg cgtagtgtgc
     4501 ccataaaggt aataaccgtt ggcatacagg gtgatattct ggattccgtt tgggaaaact
     4561 aagatggaga aaaaactaga gtcaagaata aaatatttta aatttacctg actcgagggt
     4621 tttccccatc aacctattta taaacaagct gcacagctac tacttacaaa caatatctaa
     4681 ccaatttaaa aattttgaat catgagcatg ttttgttttc agtgtcagga gacagccaaa
     4741 ggaaccggct gtacaatcaa gggggtatgc ggtaagagcg atgaccttgc caacatgatg
     4801 gacctactaa tgtttgttac caaggggatt tgcgtttaca gcaccgcact ccgggaacaa
     4861 aagggttatg aaaaccctat cgtgaacaag tttgttttcg acagcctgtt caccaccatc
     4921 accaacgcta acttcgataa gcaggtgata tccaaccggg tatcaagtgc gttggaaatt
     4981 cgggaaacat tacgtaacga agcctccctt caaggagtaa gggttgacac ctcgtttgag
     5041 ggatgcacat ggagtggcac accggcagaa tacgaagcca aggccgccct ggtaggagtt
     5101 ctcagcgaaa aaaatgagga tatacgctcg ctcaaggagc tgattacata cggggtaaaa
     5161 ggaatggcag cctataccga acatgcctac aacctaggcc atgaggatgt gcaaaactac
     5221 gccttcatgc agcgggcttt ggcctccata accagaaatc tgagcgtaga tgagctagtg
     5281 gcgctcaccc tcgaaaccgg taagtttggc gtaaacgcca tggcgctgct cgacaaggcc
     5341 aacaccagca gcttcgggaa tcctgaaata accaaggtga atctgggcgt aaggagcaat
     5401 cccggtatcc tcatcagcgg gcatgatctt aaggatatgg aggagctgct agctcaaacc
     5461 gagggaacag gtgtagatgt ttacacccat agcgaaatgc ttccggccaa ctactaccct
     5521 gcgtttaaga agtacaggca ctttgttgga aactatggca gcagctggtg gcaccagcgt
     5581 gaggatttcg agaccttcaa cggacccatt ctatttacca ccaactgtat tgtgccccct
     5641 ttaaataatg caacctataa ggacagaata tttacaacgg gtgcagcagg ctaccctggt
     5701 agcaagcatg ttgccgatcg caaacccggg caacaaaagg atttcagcga aattattgct
     5761 ttggctaaaa cctgtaaggc ccctacccaa attgaaacag gcgagattat tggaggcttt
     5821 gcccacgccc aggtttttgc acttgccgat aaggtggtag atgcggttaa aagcggagca
     5881 ataagaaaat tcgtggtgat ggccggttgt gatggtcgca tgaaggaccg aacctactac
     5941 accgagtttg ctcaaaaact acccaaggac accgttattc tcaccgctgg atgcgccaag
     6001 taccgctata acaagctagc tttgggcgac atcaacggaa taccccgggt gctggatgcc
     6061 gggcagtgca acgactccta ctcgctggcc atcattgccc ttaagctgaa ggagattttc
     6121 cagctgaacg acatcaacga gctgccaatt gcctacaaca tagcatggta cgagcagaaa
     6181 gctgttattg tgctactcgc cctgctctac cttggggtaa aaaatatcca tttaggcccg
     6241 tcacttccgg gtttcctttc accgaacgta accaacgtgc tggttgacaa ctttggaata
     6301 agcggtataa ctactgttga cgaggatatt aagctacttg tagggtaatt cttaaaattt
     6361 caaaaaaaca ccatcctgtg tgcccgggta ttcaagaaac cgcaccagga tggttaattt
     6421 taaacccttc aaaaaaaata cagccatgaa caacgagttt gaaaaggaaa agaagttcaa
     6481 ctttctggat gaggttgatt acagcacggg aggcgttgtt agtaaaaacg tgattaaaaa
     6541 accgacaggt aatatttcgc tgtttgcatt cgagaaagga gaagggctta gcgagcatac
     6601 tgcaccattt gacgccctgc tgcaggttat tgacggcgaa gctaaagtta ccataaacgg
     6661 caagccacac gacctaaagg ccggagaatc tataatcatg cctgccaaca gcccccatgc
     6721 agtttttgca gctgaacgct tcaaaatggt ccttaccatg atcaaggagt aaaccatcat
     6781 gaagcctata aaaaattcac cagcctgaac acacgttcgg gttggtggat tctaaaaaca
     6841 ttgtaaggag caataatcaa ccctattagg ctacaccaat acacagtatt tcgaagggag
     6901 tatttatccg gttttccctc caaagttttt agcttaacct tttacatgct aaaaacaacc
     6961 atttgaacca ccagtaatcg gattattagt gatttaagat tcgtcatgca acggtttttt
     7021 ttacttttaa aatgatagca aacatgaata taattactcc cgaaaatgcg gaaaaagtcc
     7081 cttttgcact caacgggcgc aagatgtaca gcagccctac tgcagaaata gttcatctaa
     7141 cactaaaacc tggcgaaacg gttgataggc actccaaccc cttcgacgta gtgttctaca
     7201 tgctcgaggg cgaggctacc atagaaattg acggggtgaa agccattctt aaaaccgatt
     7261 gttgccttga ggtaaagcgt gataggcctc gtggaatgag caataataca ggaaaagatg
     7321 tacgaatact cgtttttaag ctgttctgag a
//
LOCUS       DAOSHM010000085         7217 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_8993_length_7217_cov_20.064507, whole genome shotgun sequence.
ACCESSION   DAOSHM010000085 DAOSHM010000000
VERSION     DAOSHM010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 7217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7217
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8993_length_7217_cov_20.064507"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(543..1196)
                     /locus_tag="PK839_09700"
     CDS             complement(543..1196)
                     /locus_tag="PK839_09700"
                     /inference="COORDINATES: protein motif:HMM:NF012900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HOG73050.1"
                     /translation="MTKPRVKICCIGSIEEAKIAIEHGASALGLVGKMPSGPGVIGDE
                     LINKIARAVPPPISTFLLTSETKPKDIISHYKKVYTSTIQIVDELEDRGYELIRNELP
                     NVKLVQVIHVIDDNSVKEAIEISKYVDAILLDSGNPNLPVKKLGGTGRTHNWELSLKI
                     RESIPIPIFLAGGLNKDNVRQAIETVHPFGIDLCSGVRTNGKLDKNKLREFFNAVNE"
     gene            complement(1345..2190)
                     /gene="lgt"
                     /locus_tag="PK839_09705"
     CDS             complement(1345..2190)
                     /gene="lgt"
                     /locus_tag="PK839_09705"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOG73051.1"
                     /translation="MIVNYIHWNIDPEIINIFDISIRYYGILFVGGLVLCMLILKGIF
                     KKEGIPLNKLEKLSIYGIIGIFAGARLAHCLFYEPSYYLSHPLEIILPIQPTVDGGYK
                     FTGYQGLASHGGALGLIIALIIYARKTKESIIKTIDLIAIVAGLGACFIRLANLMNSE
                     IIGIPTNVPWAFIFERVDSLPRHPAQLYEAIAYLLIFGLMLYLYKTKRENLKNGFFFG
                     LVITLIFVTRFFIEFIKEKQVPFEEQMKLDMGQLLSIPFIVLGTGFIIHGLVKTKKNR
                     TSAQR"
     gene            complement(3124..3199)
                     /locus_tag="PK839_09710"
     tRNA            complement(3124..3199)
                     /locus_tag="PK839_09710"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3162..3164),aa:His,seq:gtg)
     gene            3335..4636
                     /locus_tag="PK839_09715"
     CDS             3335..4636
                     /locus_tag="PK839_09715"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HOG73052.1"
                     /translation="MKLDNTRALQFYQIIRFLAVFTIGMAMVKLSLPQAQVGQYEVVL
                     FIASLLTTFWITGIIQALLPMTSSSVSRDSGSKRPEIFCALVVVVALTLVSALLLLVF
                     KNLIGQLAGVANFPYFKQTILYIMLSTPTLLIEYIYLIENQPRKMIAYGLIAYSLQVA
                     MVVLPLLVDVRVELCIFGLIGISAIRMVWLLVVVRRYAELKIDRKFMFDFIAKSTPLS
                     LKYLVSSSGLYIDQLIISAYYDSSTFAAYRFGAREIPLITILTVGLSNSMLVEFGVKE
                     HIGDTLERLRRESLKLMHILFPLSVVAIILSKPLFPVIFSSDYLQSANIFMLYALLVT
                     SRTIFPQTVTMGFQQNSFALLASTLEMVLNIALSLVFIQLFGIEGVALATVVVYLFEK
                     VLLAGYNGRRLGIHPAEYIPMKPYTAYCTLTIATYLLVRFV"
     gene            complement(4709..6436)
                     /gene="lysS"
                     /locus_tag="PK839_09720"
     CDS             complement(4709..6436)
                     /gene="lysS"
                     /locus_tag="PK839_09720"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HOG73053.1"
                     /translation="MALLDELSEQEVIRRQSLDELRKLGIDPYPAAMYPVTHTTGSIL
                     SNFENLCEQQAEVCIAGRIMARRIMGSASFLELQDEKGKIQVYVKRDDICPGEDKTFY
                     NVVFKKHLDIGDIIGVKGFIFRTQMGEESVHAREITLLSKSIRPLPIVKEKDGKVFDA
                     VTDAELRYRQRYVDLIVNPQVREVFVKRTKIVNTMRDFFNSKGYLEVETPILQPIPGG
                     AAARPFITHHNTLDMPLYLRIADELYLKRLIVGGFNGVYEFSRDFRNEGMDRTHNPEF
                     TVLEIYVAYKDYFWMMDFTEEMIERVALSLHGTTAITVGDSTINFKRPFRRLTMTDAI
                     KEYAGVDITGKTEEQLRSICKEQGIEVDETMGKGKLIDTLFGEKCEQHLVQPTFIMDY
                     PIEMSPLCKRHRSNPELTERFELIVGGKELCNAYSELNDPIDQLERFQEQLRLSEKGD
                     DEAMFIDMDFVRALEYGMPTCSGMGIGIDRLTMFMTNQPSIQDVILFPQMRPEKKQLK
                     DPVEAYSQRNIPAEWVPVLQKLGYSTVVSLTNVAPGKLFNDICGYNKKNKLGLQNPSM
                     DDVKRWVEQ"
     gene            6995..>7217
                     /locus_tag="PK839_09725"
     CDS             6995..>7217
                     /locus_tag="PK839_09725"
                     /inference="COORDINATES: protein motif:HMM:NF025652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4372 domain-containing protein"
                     /protein_id="HOG73054.1"
                     /translation="MGKDTAKNLASQPVFKQVMKILPREQFDIVVRQCGSDCCYKSFF
                     SWDQLVVMLFGIFSRCDSMGEVCDGMRALG"
BASE COUNT         1896 a         1603 c         1509 g         2209 t
ORIGIN      
        1 ttatttccat ttttggatgt ataggcgcca taaagttgcg tttaccattc aatgctatag
       61 taaaaaaatt acatttgaaa caaatatatg taatttttct gcaactcatc aaatgggatt
      121 ctaatttcct gtaaattttt ttggctcgca tgggtgtatg catctgtaac ttgaccactt
      181 ttatggcctg gtatcaaccg gataaaccgc agggggtcag cttcgagcag gtattcggaa
      241 cggctggccc catcgctgcc gtacggttgc cttcggcgag ctctccgcca gctggcggat
      301 cggttactgt cgtgtggcag cctcacccca aaccctctgc cactagactt tacacatcta
      361 ttttttaatt cacaaagcac aataccacca gtttacctct tttttctacc acctgtttcc
      421 ttctttttct atgcttcact accccacgag aggactcgcg cagtagcggg gcagagtaga
      481 atacctgatt taaccactta aaatccagaa atttatattg ctggtaacgc cttatttttt
      541 tgctattcat taaccgcatt aaaaaattct ctcagcttat ttttgtcaag ttttccgtta
      601 gtacgaacac cgctgcagag gtcaatgcca aacggatgaa ctgtttcaat tgcctgtctt
      661 acattatctt tatttaaccc acctgcaagg aaaattggaa ttggtattga ttccctgatt
      721 tttaggctta attcccaatt atgtgttctc cctgttccgc ctagcttttt tactggtaaa
      781 tttggatttc cgctgtccaa taaaattgca tcaacatact tggaaatttc aatggcttct
      841 tttacagaat tatcgtcaat aacatgaata acttgcacta gttttacatt cggtaactcg
      901 ttacgaatca gctcataacc tctatcttcc agctcatcta caatttgaat tgttgatgtg
      961 tacacttttt tgtaatgcga gataatgtct tttggcttag tttcgcttgt cagcaagaaa
     1021 gtagaaatcg gtggaggaac agcccttgca attttattta ttagctcatc accaattaca
     1081 cccggtccac ttggcatttt cccgactaag cccaaagcag atgcaccgtg ttcaattgcg
     1141 atttttgctt cttcaatgct acctatacag cagattttta ctctaggttt tgtcatggcg
     1201 tgccgtttaa gtcgtttttt taagggtgat gtagaacaaa tggtgtcatg attagttgcc
     1261 gaattcgaag caattccgga tcaagtaaca ccaaactttg ataagagcta taaccttggg
     1321 attggcgctg cacaggcaat agctttaccg ttgtgcgctg gtccgatttt tttttgtctt
     1381 taccaatcca tgaattataa atccagtacc taaaacaata aacggaatac tcaacaattg
     1441 tcccatatct aatttcatct gttcttcaaa aggaacttgc ttttccttta taaattcgat
     1501 aaagaaacgg gttacaaaaa tcaacgtgat taccaaccca aaaaagaatc cgtttttaag
     1561 gttttcccgt ttagttttat aaagatatag catcaatccg aatatcagta aataagcaat
     1621 tgcttcgtat agctgagccg gatgtcgtgg caaactatct actcgctcaa aaataaaagc
     1681 ccacggaaca tttgttggaa ttccaataat ctcagaattc atcaagttgg caagtcttat
     1741 aaagcatgct cctaaacctg caacaattgc tattaaatca atcgttttta taattgattc
     1801 ttttgtcttt ctggcataaa ttatcaaagc aataattaaa cccaatgctc ctccatgact
     1861 ggctaaccct tgataaccag tgaatttata tccgccatca acagtcggtt gaattggtag
     1921 aataatttct aatggatggc tcaaatagta tgatggctca taaaataagc aatgagcaag
     1981 tctagctccg gcaaaaattc ctattattcc gtaaattgaa agtttttcta atttattcaa
     2041 cgggattccc tcttttttaa aaataccttt taagattaac atgcaaagca ccaatcctcc
     2101 cacaaatagg atcccatagt atctaatcga aatatcaaaa atattgataa tctcagggtc
     2161 tatattccaa tgaatataat ttactatcat aatttttctt tgtcttgttg ggtctgctaa
     2221 tcacgtaacc aaggttcgaa gttaacaaaa gttgtgaaaa tcgttaacaa aattttttat
     2281 ttgagtaaaa ggcaacttgc ttacacctta tccttactcc ctttaaacac ttatttttgt
     2341 tagcggttgt tgttcttttt atcaacatat ttgaaatttt tatgtccatc ttgaacctca
     2401 atcagttcaa tgttttttat tcgtatttgt tcggtataat aaataaatct cgaaccatct
     2461 ccttttagaa tagaatcctg tacgaacatt tgctcaaaat agaaataaat tcttttgtta
     2521 tttataccag taatgcgaac ttaaattgtt gggctactct ttaattcaaa tggactatta
     2581 tctttatcta cacattttat gtagtaaaaa ggatatgttt tataagtaac aacatctccc
     2641 attgaacctc ttgcatctac ggtttttaat tcaaccgaat caatggcttt aaattgttca
     2701 ttaaaacttt tcaatcggga tataatatgt tttacagctc gaaattatca ttgtgaaaac
     2761 catgatgaaa atcacattaa agcccttatg tctcataatg cggtaaaatt tttattaata
     2821 catcgtttgt cgtaattaaa ccctattgat tcatacatct atcttgctgt atgtgtgttt
     2881 tttataataa ccactaactc actcgtatgt aataccattc attttgggtt taagaatgat
     2941 gcaggcatca catgcactca acaaataaat gattattttc cttacttttc gcctgccata
     3001 attctgtgca ggaccataag gacgcaaacg gtttatccat gcagcatggg ggggcgctgc
     3061 atttccgggc atggagtggg tgtggaatgg agcaaaaaaa aaccggagtt gcctccggtt
     3121 caggggtgga agatgggact tgaacccacg gcccccagat ccacaatctg gtgctctaac
     3181 cagctgagct acatccacca tgtgatttag gctgcaaagt tataaaattt cttcaacctg
     3241 aaataaaaaa atggggaaac tttgttgccc atgtcctggc tgcgagctgt ttttttaata
     3301 ctttagcatc ctgtagccat ttttgatacg taaggtgaag ctagacaaca caagagccct
     3361 tcagttctac cagattatca ggtttctggc tgttttcacc attggtatgg ccatggtgaa
     3421 gctatcgctt cctcaggcgc aggttgggca gtacgaggtg gtgctgttta ttgccagcct
     3481 ccttaccacc ttttggatta ccggaattat tcaagccctt ctccccatga cgtcttcatc
     3541 tgtcagcagg gattcgggtt ccaagcgtcc tgaaattttc tgcgctctgg tagtggttgt
     3601 ggcgttaacc cttgtgtcgg ctttgctgct gctggttttt aaaaacctga tcgggcagct
     3661 ggcaggggtg gccaacttcc cttacttcaa gcaaacaatc ctgtacatca tgctgagcac
     3721 cccaacgctc ctgattgagt acatctacct gattgaaaat cagcctcgta aaatgattgc
     3781 ctatgggctt atagcctact cgttgcaggt ggccatggtg gtgttgcccc tgctggttga
     3841 tgtcagggtt gagctttgca tcttcggttt gataggcatt tcggccatcc ggatggtgtg
     3901 gctgttggtt gttgttcgcc ggtatgcgga gctaaaaatt gaccggaagt tcatgtttga
     3961 ttttattgca aagagcacgc cgctatcgtt gaagtacctg gttagcagct cgggcctcta
     4021 cattgaccag cttattattt cagcctacta cgattcgtca acctttgcag cttaccggtt
     4081 tggcgccagg gaaattccgc taattaccat actcaccgtt gggctgagca actccatgct
     4141 ggtagagttt ggggtaaagg agcacattgg cgataccctg gagaggctca ggagggaatc
     4201 gctgaagctc atgcacatct tgttccccct gtcggtggtg gcgattatct taagcaagcc
     4261 cctattcccg gtgatattta gctccgacta cttgcaaagc gccaatattt tcatgcttta
     4321 tgccctgctg gttaccagca gaactatttt cccgcaaacg gttaccatgg gatttcagca
     4381 aaatagtttt gcgctgctgg cctctacgct cgagatggtg ctaaacatag cattaagcct
     4441 agtatttata caattatttg gaattgaagg ggttgcgctg gctacggttg tggtttacct
     4501 ttttgaaaag gtgctgcttg ccggatataa tgggcgtagg ttgggtattc atcctgcaga
     4561 gtacatacca atgaaaccct ataccgccta ctgtacactt accattgcca cctacctttt
     4621 ggtacggttt gtatagcggc ataacgctac actttcctgc catttaagaa gcaacctgcg
     4681 aatttcaccg ggctatgaat tcatacacct actgctcaac ccagcgttta acatcgtcca
     4741 tacttggatt ttgcagcccc agcttgtttt tcttgttata gccgcaaatg tcgttaaaca
     4801 gcttacccgg agcaacattg gttagcgata caacggtgct gtagcccaac ttctggagaa
     4861 caggcaccca ctcagccggt atgttccttt gggaataggc ctcaacaggg tctttcagct
     4921 gttttttttc agggcgcatc tgagggaata ggatcacatc ctgaatggag ggctggttgg
     4981 tcataaacat ggttaaccgg tcaatcccaa ttcccattcc cgagcaggta ggcataccat
     5041 attccagggc acgaacaaag tccatatcaa taaacatggc ctcgtcgtcg cccttttcgc
     5101 tgaggcggag ctgttcctga aatcgctcaa gctggtcaat ggggtcgttg agttcgctgt
     5161 aggcgttgca aagttccttg ccgcctacaa tcagctcaaa acgttcggtt agctcggggt
     5221 tgctacggtg gcgtttacac aatggcgaca tctcaattgg gtaatccatg ataaacgttg
     5281 gctgaaccag gtgctgctcg cacttttcac caaaaagggt gtcaatcagc ttgcctttgc
     5341 ccatggtttc gtccacctca ataccctgct ccttgcatat gcttcggagt tgttcctcgg
     5401 tttttcccgt gatatcaacg ccggcgtact ccttgatggc gtcggtcatg gtaagccggc
     5461 ggaagggtcg cttgaagttg atggtgctgt cgccaacggt tatggctgtt gttccatgca
     5521 gggataaggc aacccgctca atcatctcct cggtgaagtc catcatccaa aagtagtcct
     5581 tgtaggccac gtaaatttcc agcacggtga actcggggtt gtgggtgcgg tccatcccct
     5641 cgttgcgaaa atccctggag aactcgtaaa cgccgttaaa cccaccaaca atcaggcgtt
     5701 taaggtataa ttcgtcggca atgcggaggt aaagtggcat gtcgagtgta ttgtggtggg
     5761 ttataaacgg ccgggcagct gcacctcccg gaataggctg taatataggg gtttcaacct
     5821 caaggtatcc ctttgagtta aagaaatcgc gcatggtgtt aactattttg gttcgtttaa
     5881 caaacacctc gcgcacctga gggttaacga taaggtcaac atagcgctga cggtacctga
     5941 gttcagcgtc ggtaaccgca tcgaacacct tgccatcctt ctccttgaca atgggtagag
     6001 gcctgatgga ctttgaaagg agggtgattt cccttgcgtg aacagactcc tcacccatct
     6061 gggtacggaa aataaatccc tttaccccga tgatatcgcc aatatccaga tgtttcttga
     6121 atacgacgtt gtagaaggtt ttatcttctc ctgggcaaat atcgtcacgt ttaacgtaaa
     6181 cctgaatttt ccccttttcg tcctgaagtt caaggaacga agcgctgccc ataattctgc
     6241 gtgccatgat tcggcccgca atgcaaacct cagcttgttg ttcgcaaagg ttttcgaagt
     6301 tggaaagtat gctgccggtt gtgtgggtaa ccggatacat ggcagcaggg taggggtcta
     6361 ttcccagctt ccgcagctca tctagtgatt gcctacgaat tacctcctgc tcgctcagct
     6421 cgtcaagtaa agccatacta ataggtgatt aggtaaaaac cgtgcaaatt tacccgaatt
     6481 ttttataacc agaagaaaat gtaccgaatc aatgatcaac aagccggatt aaaacagtgc
     6541 taacggattt actcgttaca gcagtttcta ccacgtcagg gggtcaggac gcggcaatgc
     6601 tggatactta cgctttcggg taaaggatgg tggcaaaagc aaggcactcc ccaagcccat
     6661 gaggctacgg gaactagctg gttcaaagaa cttggtttat acagattatc attacacatt
     6721 acactacaaa gcattgcacc attaaaggaa accgccgtgt ccgccgcagg cggatgcacg
     6781 gtggtgtaag ggggcgagga gtaatccccc ctacctactc gatttaaaat cctatattcc
     6841 tttgttcctg aaaacctgga cttttattcg gttctaaacc taaaatttta gttttaagtc
     6901 tctactgacc ggttaaaatt tcagtgaaaa agggactact cccgcgaggt ttcgccccga
     6961 tgttgtaggc ttgttttgct aaaaaaactg caacatgggc aaagatacag caaagaattt
     7021 agccagtcag ccggttttta aacaggttat gaaaattctt ccgagagagc aatttgatat
     7081 tgtagtgagg cagtgcggca gcgactgctg ctacaagtcc tttttttcat gggatcagct
     7141 ggtggtgatg ctattcggga ttttttcccg ctgcgattcg atgggcgaag tatgtgatgg
     7201 gatgcgggct ttgggag
//
LOCUS       DAOSHM010000086         7208 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_9009_length_7208_cov_19.860618, whole genome shotgun sequence.
ACCESSION   DAOSHM010000086 DAOSHM010000000
VERSION     DAOSHM010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 7208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7208
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9009_length_7208_cov_19.860618"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            61..2301
                     /locus_tag="PK839_09730"
     CDS             61..2301
                     /locus_tag="PK839_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HOG73055.1"
                     /translation="MAVSNENLNGQKLNLEILIAMKFKPKSLFVVAVALVAISSTAIA
                     QKQAITLDDLFKKGTFRARGVYGLTSMNDGKSYSAYNSKGQVAKFEYSTGKEVEVLFD
                     PATVNVEDMNSFSGYQLSPDETIMLVETNVESIYRHSYTAVNYVYNLKSREIKPLSVN
                     GPQMNAAFSPDGRMVAFVRNNNIYMVNLTTAEEFQVTTDGLYNSIINGAADWVYEEEF
                     SLTTGLQWSPDSRKLAFYRFDESRVKMFNMTKYEGNLYPSNYTFKYPKAGEENSVVSI
                     HVFNLDSKSTIKMDVGPETDQYIARIKWSADPEKLCMVRLNRLQNKVDILLADASTGS
                     SSVLYTETNKYYIEEVTDAYPTFTPDGKYFIITSEKDGYRHIYLYGMNGSLVNQLTAG
                     INEVTELYGYSPLSKMVYYQAYDDSPLCKAVYAKKIDGKQPVKLSTNIGNNYAQFSSN
                     FEYCMIFHSNIQEPLTVSLINAKGKEVRVVEDNQKLRDRLKEFAIPSKEFYTFTTSEG
                     VELNGYMIKPVDFDSTRKYPVMMYQYSGPGSQTVTNSFRVGWDEYLATLGYLVVCVDG
                     RGTGGRGEAFRKMTYGQMGYYESIDQIEAAKYLQTLSWVDAGRIGIWGWSYGGYMSSL
                     CLFKGADIFKMAIAVAPVTNWRYYDSIYTERFMGLPQDNPLGYDSNSPINHADKLRGK
                     LLLVHGTADDNVHFQNSMELVERLVQSGKQFDMMIYPDKNHGIYGGNTTMHLYTLLSN
                     YVQENL"
     gene            2373..2456
                     /locus_tag="PK839_09735"
     tRNA            2373..2456
                     /locus_tag="PK839_09735"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2407..2409,aa:Leu,seq:cag)
     gene            2572..2658
                     /locus_tag="PK839_09740"
     tRNA            2572..2658
                     /locus_tag="PK839_09740"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2606..2608,aa:Leu,seq:gag)
     gene            2715..3449
                     /locus_tag="PK839_09745"
     CDS             2715..3449
                     /locus_tag="PK839_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010184560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOG73056.1"
                     /translation="MRKVKLTLLLVSAFTGSFAQSADSFTTPNGKSLRITFIGHGTLM
                     MEYENLVIHVDPWSKLASYDKLPKADIVLVTHHHADHLDSAALVKVCKVGTEIYWTQT
                     CSGSSSLKPKSTIMHNGDRLTTHGVEISAVPAYNLIHMTPGGTPFHVKGEGNGYILNF
                     DGFVVYVAGDTEDIPEMKGFGRVDIAFLPMNLPYTMTPEMVHNAATMLHPKILYPYHY
                     GDTDTNALLNIMKTMPEVDVRIRNLK"
     gene            complement(3793..6672)
                     /locus_tag="PK839_09750"
     CDS             complement(3793..6672)
                     /locus_tag="PK839_09750"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOG73057.1"
                     /translation="MEPFLKLVAKDLYNLLGSELSDITLVFPNRRASLFFARYLAEQL
                     SSPTWQPAFTTVNELMYKVAGLRPANNLWLNYKLYQSYTDITGINQPFDDFYFWGNVM
                     LSDFDQVDKYQVDPQKIFANIKDIKDIDRRFDEFDAEHVEAIKKFLNLIDDHGSSAIR
                     SRYSEIWSKLGVIYSQLRERLLQEGTAYEGLAYRMASEEMNKSQLLTLNERFALIGFN
                     ALNACEKTLFNHLKNFNNALFYWDYDKYYMQNQDYVHEAAFFMSDNLKSFPGRLDSSF
                     FDSLQGGKDKNVNLIASPSKVAQAKIVSNILNKLGYDDENLGINTAIVLPEEQLLLPV
                     ISALPTNLDEVNITMGYPINDTFAYSLADGLISLQANLKTAGTEVKFYNKDVLNLLSH
                     PYVVQLAKEESKSISATIVQQKSISVDGSYFKDSPLLSKIFSQASYLPTYLLDVIKTV
                     SGGLASLENDRPESIKLELEFLFAIYKALNGLDGIISQFDTTPSPRTFRSIFRRAMAE
                     QRVSFYGEPLSGIQLMGFLETRSLDFENVIILSLNDGVLPGPSNSPSFITPSIRKAYG
                     LPDYRHQNAIYAYYFYRLLQRASNIYMVYTNSSESMRTGEVSRYVLQLLMESNLKINR
                     IQVKFDLGITPDGPISVSKTDRVMAILERYLANGQSSKYLSPTAMVAYKNCKLRFFFS
                     HVAGFKEPEEMDEAIDERGVGIILHRAIELIYDGIKSSVTSTSVGAILKNDSLIEANI
                     RKAYAENFNVDLSKFDEVVIGRNALLLERIKWMVRQMLVVDMKRTPYSITRSEQSEMV
                     DIPIVVDGRNFVVKLGGRIDRVEQANGLTRIIDFKTGKFEPNKVTFSEIDQLFENKSN
                     TDGILQLFAYCMIYSMKYGIDAGSIQPNLWYVRKNTLPLISKSLKKGNSAKTEVTTFE
                     SLQAPFRQMLVALLEEIFNPSVDFTQTDNTDNCKICPYNAICNRV"
     gene            complement(7008..>7208)
                     /locus_tag="PK839_09755"
     CDS             complement(7008..>7208)
                     /locus_tag="PK839_09755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG73058.1"
                     /translation="GTTRPACGVAQTVPLGHSRATFHPRPICGSGFYAILLIQATFND
                     SCISEQRGECLSPESAEDKDKG"
BASE COUNT         1954 a         1582 c         1636 g         2036 t
ORIGIN      
        1 ttttatcctt taccctttat ctttatactc cgttatgttt atttaaacta tttggctaca
       61 ttggcagtca gtaatgaaaa ccttaacggg caaaaactaa acctagaaat actgattgcc
      121 atgaagttta aaccaaaatc actatttgtg gttgctgttg cacttgttgc catcagcagc
      181 acggctattg cacaaaaaca ggcaattacg cttgatgatt tgtttaaaaa aggcaccttt
      241 cgtgctcggg gagtttacgg attaacctcc atgaacgatg ggaagagcta cagcgcctat
      301 aattccaaag ggcaggttgc caaatttgag tattctaccg gtaaagaggt tgaagtgctc
      361 ttcgaccctg ctactgttaa tgttgaagac atgaactcat tttcgggtta ccagcttagt
      421 cccgatgaaa ccataatgct tgtcgaaacc aacgttgaga gtatttatag gcattcatac
      481 accgcagtta actatgtata taaccttaaa tcgagagaaa ttaaacccct ttcggtcaac
      541 ggtccacaaa tgaatgcggc cttctcgccc gatggccgga tggttgcttt tgtcagaaat
      601 aataacatat acatggttaa tcttaccact gccgaagagt ttcaggttac taccgatggt
      661 ttatacaaca gcataatcaa cggtgctgcc gattgggttt acgaggagga attttcgctt
      721 accaccgggc tacaatggtc gcccgactca aggaaactgg cattttaccg attcgacgag
      781 agtcgggtga agatgttcaa catgaccaag tacgagggca acctttaccc ttcaaactat
      841 acctttaaat atcctaaggc gggcgaggag aactcggtgg tttccattca tgttttcaac
      901 cttgattcta aatctaccat taaaatggat gttgggccag aaacagacca gtacattgcc
      961 cgaattaagt ggtcagctga tcctgaaaaa ttatgtatgg ttaggctcaa ccgtcttcaa
     1021 aataaagtag atattctttt ggccgatgca tcaaccggca gttcaagtgt gctttatacc
     1081 gaaacaaaca agtactacat tgaagaggta accgacgcct acccgacctt cacccccgat
     1141 ggtaagtatt ttattattac ctccgaaaag gatggttacc ggcacattta tctttacggc
     1201 atgaacggca gccttgtaaa ccagcttact gcgggcatta acgaggtaac agagctgtac
     1261 ggctacagcc ctttatcaaa aatggtttac taccaggcct acgacgattc gcctctttgt
     1321 aaggcagttt atgctaagaa aattgacggt aaacaacctg taaagctgtc aacaaatatt
     1381 ggcaacaact atgctcagtt cagcagcaat ttcgagtact gcatgatttt ccactccaac
     1441 atacaagaac cgcttactgt ttcactgatt aacgccaaag gcaaggaggt tagggtggta
     1501 gaggataacc agaagttaag ggataggctt aaggagtttg ccattccctc aaaggaattt
     1561 tatacattca caaccagcga gggcgttgag ctcaacggtt acatgattaa accggttgat
     1621 tttgactcca cccggaagta ccctgtaatg atgtaccagt acagcggtcc cggatctcaa
     1681 acagtaacca acagctttag agttggttgg gacgaatacc tggccactct tggatacctt
     1741 gttgtttgcg tagatggacg aggaaccggt ggaaggggag aagcgtttag gaagatgact
     1801 tacgggcaaa tgggttacta cgaatccatc gaccagattg aggctgcaaa gtacctgcaa
     1861 accctttcat gggttgatgc aggaagaatt ggaatttggg gttggagcta cggtggatat
     1921 atgtcgagcc tttgcctttt caagggagcc gatattttta aaatggccat tgctgttgct
     1981 ccggttacca actggcgtta ctacgattcc atctataccg aacgatttat ggggctaccc
     2041 caggacaatc ctctgggcta cgattccaac tcaccaatta accatgccga taagcttcga
     2101 gggaaacttt tgctggtgca tggcactgcc gacgacaacg ttcactttca gaacagcatg
     2161 gagctagtcg aacgacttgt gcaaagtgga aaacagttcg atatgatgat atatcctgat
     2221 aaaaatcatg gcatatacgg gggtaacacc accatgcacc tctacacgct cttgagcaac
     2281 tacgtacaag aaaatttata gtgttcctgc taaatttccg ggtgtcgtgt ttggatgaat
     2341 cagaaattgt ataattttgc atccgcattt tagcccagat ggcggaattg gtagacgcgc
     2401 tagtttcagg gactagtatc agcaatgatg tgcaggttcg agtcctgttc tgggcacaag
     2461 gttcacccca tggtgaacct ttattacgaa aacgccaaaa atatcgccct ggtgcggtag
     2521 aaatttgaaa aaataggcta tttttgcatt cgttaattcc cagttgttat tgcccaggtg
     2581 gtggaattgg tagacacacc agcttgaggg gctggcgccg tcaaaagcgt gcaagttcga
     2641 gtcttgtcct gggcaccaaa ccttgtaatt tttgcaaggt ttttttattt ttgggtaaat
     2701 caacatctaa taccatgaga aaagtaaaac ttaccctttt gctggtttcg gctttcaccg
     2761 gctcgtttgc ccaaagcgcc gactcgttca ccactcccaa cggcaagtcg cttcgaataa
     2821 ccttcatcgg tcatggaacc ctgatgatgg agtatgagaa cctcgtaatt catgttgacc
     2881 catggagcaa gctggctagc tacgacaagc ttcctaaggc cgacatagtg ctggttaccc
     2941 accatcatgc cgaccacctt gactcagccg cactggttaa ggtgtgcaaa gtaggtaccg
     3001 aaatttactg gacacaaacc tgctcgggga gcagtagcct taaacccaaa tccacgatta
     3061 tgcacaatgg tgacaggctt actacacatg gtgttgagat ttctgcggtt cccgcctaca
     3121 acctcattca catgacaccc ggcggaactc cttttcatgt aaagggcgaa gggaatgggt
     3181 atatactgaa tttcgacggg tttgtagttt atgtagcagg cgataccgag gatattcctg
     3241 aaatgaaggg cttcggaagg gttgatatcg cctttttacc catgaatctt ccttacacta
     3301 tgacccctga aatggtacac aacgcagcaa ccatgctaca tccaaagatt ctttatccct
     3361 accattatgg cgataccgat accaacgccc tgttaaacat catgaaaaca atgccggaag
     3421 ttgacgtccg tattaggaat ttgaaatgat ttaacaaaaa agttgtccgg attatactgt
     3481 caggacctgg gactttggtg gattcgatgc atggcgacag caacttttga actccggaca
     3541 acttggctgc tggtggcagc agtagtatat tgtttgtgtt tgggttactt ctatagtaga
     3601 aaaatcgtgc caccattgcc ggaacccaag ctgtttttta taacatactg catatctgca
     3661 atataatctt atccttccgc atttctagct gttgcctgct gccattttga ggaataaaat
     3721 ccccaaattg tagagttttg aggagtggtt gacctgttgc tttccacact catgcgtttt
     3781 ttcttgacaa aattataccc tgttgcagat ggcgttatac gggcatattt tgcagttatc
     3841 ggtgttgtcc gtttgggtaa aatcaacgga ggggttgaaa atttcttcca gcaaagccac
     3901 cagcatttgt ctaaatggtg cttgtaagga ttcgaaggtg gtcacttcag tcttagcgct
     3961 gtttcccttt tttaaagatt tggatataag gggaagagta ttttttcgca cataccaaag
     4021 gttgggttga atgctgccgg catcaatgcc gtacttcatg ctgtaaatca tacagtaagc
     4081 aaatagctgg agtatgccgt cggtgttgct cttgttttca aaaagctggt ctatttccga
     4141 aaaggttacc ttgttaggtt caaacttacc ggttttaaag tcaattattc gggttaggcc
     4201 atttgcctgt tcaaccctat caattcgtcc acctagcttc actacaaaat ttcttccatc
     4261 cacaacaata ggtatatcaa ccatttcgct ctgctcagac cgggtaatac tgtatggagt
     4321 tcgcttcatg tcaacaacca gcatttgtcg aaccatccat tttatccttt ccaatagcag
     4381 ggcgttgcgg cctatgacaa cctcatcaaa ctttgaaagg tcaacgttga aattttcagc
     4441 gtaagccttc ctgatatttg cttcaattaa actgtcgttc ttcaaaatgg cgccaacgct
     4501 cgtactggta acgcttgatt tgataccatc atagatgagc tcaatagctc tatgaagaat
     4561 tatccccacc cctcgctcgt ctatggcctc atccatttcc tccggttcct taaagccggc
     4621 aacatggctg aaaaagaaac gcagcttaca gtttttgtag gcaaccatcg ctgtgggcga
     4681 gaggtacttt gaactttgtc cattggctaa atacctttcc agaatggcca taaccctatc
     4741 tgttttgcta accgatattg gaccatcagg ggtaattccc aagtcgaatt ttacctgtat
     4801 tcggttgatt ttcaagttcg actccataag gagctgcagc acgtaacggc ttacctcgcc
     4861 cgtgcgcatg ctttcggagc tgttggtgta aaccatatag atgttgctgg cccgctgaag
     4921 caagcgatag aagtagtagg cataaattgc attctgatga cggtagtcgg gcaacccgta
     4981 agctttcctt atgctcggag tgatgaatga tggagagttg gaaggtccgg gcagcacccc
     5041 atcgttcagc gaaaggataa tcacattttc aaaatcgagg gagcgggttt ccaaaaagcc
     5101 catcagctgg atgccggaca acggttcacc gtagaatgag acccgttgct cggccatcgc
     5161 cctgcgaaat attgacctga aagtccttgg tgaaggagtt gtgtcgaatt gggaaataat
     5221 tccatccaac ccgttcagcg ccttataaat ggcaaacaga aattccagct ccagctttat
     5281 gctctccggc ctatcgtttt ccaacgatgc tagtccaccg gaaacagtct taataacatc
     5341 caggaggtag gtgggcaggt atgatgcttg agaaaagatt ttggatagta atggtgaatc
     5401 cttaaaataa ctcccgtcaa cactaattga tttttgctgc acaatggtgg ctgaaattga
     5461 ttttgattcc tcttttgcta gctgcaccac gtagggatgc gataataaat tcagaacatc
     5521 cttgttgtag aatttcactt cggtgccggc ggtttttaga ttcgcctgaa gcgaaataag
     5581 cccatcggcc agactatagg caaaagtatc gtttatggga tagcccatgg tgatattaac
     5641 ctcatccagg ttggtgggta gtgcagaaat tacgggcagc aggagttgct cctcaggcag
     5701 cacaatggca gtgttaatac caaggttttc gtcatcataa cccagcttat tcagaatgtt
     5761 tgaaacaatt tttgcctgtg ccacctttga gggagaggca ataaggttaa cgtttttatc
     5821 tttaccgcct tgcaagctat cgaaaaacga tgagtccagc ctgccaggaa aagatttcag
     5881 gttatcggac atgaaaaaag ctgcttcatg cacgtagtcc tgattctgca tgtagtattt
     5941 gtcgtaatcc cagtaaaaca gggcgttatt aaaatttttc aggtgattaa atagtgtttt
     6001 ttcacaagcg ttgagcgcgt tgaacccaat tagggcaaat cgttcgttta gggtgagcag
     6061 ttgggatttg ttcatttctt cagaggccat cctataggct aagccttcgt atgccgttcc
     6121 ctcttgcagc aatcgttcac gtagctggga gtatatcact ccaagcttcg accaaatttc
     6181 ggagtaccgt gatcgaatag ccgagctgcc gtggtcgtca atcaggttca aaaacttctt
     6241 aattgcttca acatgctcgg catcaaactc atcgaatcgg cgatctatat ccttgatatc
     6301 cttaatgttg gcaaatattt tttgggggtc aacctggtac ttgtccacct ggtcaaaatc
     6361 cgacagcatc acgttacccc aaaagtagaa atcatcaaaa ggttgattta ttccggtaat
     6421 atccgtgtag gattggtaaa gtttatagtt aagccacagg ttatttgcgg ggcgcaggcc
     6481 tgctactttg tacatcaact catttactgt agtaaaggca ggctgccatg tgggcgagct
     6541 tagctgttcg gccaggtacc tggcaaaaaa gagagatgcc cgtcggttcg ggaaaaccaa
     6601 cgtaatatcg gatagctcac tccccagtag gttgtaaaga tctttggcta caagttttag
     6661 aaacggctcc aatgatgggt agttttagtt tgacatgaag cattataggc tgttgtgctc
     6721 tattgactta gctataccag ttattaatcc aacatgctaa tgtagttacg attggtgata
     6781 taatttgcta gggttgaaaa agtttttttg aaaaaatttt ggtttatttt ccacaactcc
     6841 tgaattcttt tacccctgtc ttcagcccca actgggcgga atagctagca aggggcgtaa
     6901 gccctttgtc aacgaggcag catggtgtat taagccctga aagggcggaa taattttggc
     6961 gaatccgcca atgttccgcg ggtaggcgaa caagtttttc ttatacttta tcctttatcc
     7021 ttgtcttcag cactttctgg ggaaaggcat tctccgcgtt gctcggaaat gcaggaatca
     7081 ttaaacgttg cttgtattag taaaatggca taaaagcctg acccgcaaat gggtcggggg
     7141 tggaacgtag cgcgggaatg cccaagcggc actgtttgag ccacgccgca ggcggggcga
     7201 gttgtgcc
//
LOCUS       DAOSHM010000087         7146 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_9127_length_7146_cov_20.909886, whole genome shotgun sequence.
ACCESSION   DAOSHM010000087 DAOSHM010000000
VERSION     DAOSHM010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 7146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7146
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9127_length_7146_cov_20.909886"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            393..1103
                     /locus_tag="PK839_09760"
     CDS             393..1103
                     /locus_tag="PK839_09760"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOG73059.1"
                     /translation="MSYKCVLVDDEPLAVGVLEGFLRHIPDIEVVATFNEAIPLFDFI
                     QHHKVDIIFLDIEMPNLSGIDFLKCLKDPPFVIITSANRDYAVDGYELNVVDYILKPL
                     TMERLVRAVSRAAEALELKARNVTAAPDAEYIFLKEGKKFIRVKVDEIQYIESIKDYV
                     KVVAEGKTVVTRMNISAVEKLLDPAEFIRVHRSFIISKKHIDAYTATSIEIGSVEIPV
                     GRIYRDEAVRRLGYDFEV"
     gene            1294..4581
                     /locus_tag="PK839_09765"
     CDS             1294..4581
                     /locus_tag="PK839_09765"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG73060.1"
                     /translation="MSSRFTLHFNVTMSIYTRYILAVVLALIGYPKMPQAQERGLFLS
                     RYFPASEYNGSTQNWCAVEDNRGVMYFANGQGVLEYDGATWNLIRVDNESAIRSLAAD
                     SSGRIYVGAFGEMGYLYPNRSGQLRYMSLMSLLDKNHSRFSQIWNIYTFGDTAFFLSD
                     SCLYRYCNGKFSYWTSQEGCFYFAFKFNNHLYIQETGVGLQQFDADSLVLVEEGNLLA
                     SQRIHAILPFGNEMLVCTRSNGLFLFRNEGVKNRLTPVSHLSGRALAVDRYFKEYSFY
                     HGVAINDNLLALSSINGNLLMVDTTWLVREVIDYRTKGVKSPVNYLYLQKNNLLWLAL
                     DNGICVVEINSPFRYWTDDMGVNGVVTDVAELGGFLYVSTQVGNFFLDEKKEEDFMPD
                     MFKKVEGDFEQSWEYLYFKVSSNLFPQGHSRPADTLLLVATRTGVYRINGSRSSRISR
                     YEPVFCFHQSKANPAALLVGLSSGIAIVEYRDGFWVDKGFVPGIKENIEQIAEDLDGN
                     VWLKASFSGVYRLKNLFFGSSFKGSLVVEKYDTSSGLPDLKSLYMTSHADSLFFYSQN
                     GYYTFNERLRQFQAYNPANNRLFSAENVDTLAWRRVWNNIISGFYVAHTSDSIIWFNT
                     PKGLFRYTPSSYTRCFDAPATFIRKVVNLDSTLFYGTNFESSKVDSAAGKPAFLPSHN
                     PLVDLGTVLEYKRNSLIFNYASPYFEGDDPVQFCYQLVGYDKLWSAWTTSNRKEYTNL
                     PEGFYTFKVKAKNYMGIEGAPAEFRFRVKPPWYRTALAYFGYMIIAFLLIAGIIRAYT
                     YHLVLEKNKLEQLVAERTQEILQQKEEIQVQAEHLKSANERILLKNAELEARKNEIEN
                     QKNKLEESYTTMNTFFGVIAHDLRNPISILVNFMGILLSDFDSMDRKKVVAYIEELNK
                     LALNTYNLLENLLDWSKSQMGSISCNLKPVNLKSLVIQNVELVQGKIEQKKVNLRVMI
                     PEECIVSADENMLHTIVRNLISNALKYTYDNGEILIYAELRNSTCYLSVKDNGVGISS
                     ELKGKLFKADRDATTPGLHNEKGSGLGLILCKTFVEQMGGNILVESQPGLGSTFTVTL
                     KLEGIPG"
     gene            complement(4603..5010)
                     /locus_tag="PK839_09770"
     CDS             complement(4603..5010)
                     /locus_tag="PK839_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyketide cyclase"
                     /protein_id="HOG73061.1"
                     /translation="MTTYESKIVAISKRAEDIYGMLSDFRNFTPMVAQRVEEWQAEEQ
                     RCSFKYQGKGPVGLKIVEKEKNKTIKITGDDNLPMQFYLWIQLKEVAPYDTRMKITLK
                     AELNMMMKMLLGKKLDEGVNAMAEGIAAAFNTI"
     gene            5083..6081
                     /locus_tag="PK839_09775"
     CDS             5083..6081
                     /locus_tag="PK839_09775"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HOG73062.1"
                     /translation="MVKFILQQSLNPYFNIASEEYLLRSFSDDFIYIYRCNPSIVVGK
                     HQNTFAEVNIRLVERLSIPVVRRLSGGGTVFHDLGNINFCFISNGVPGQLVDFKRFTL
                     PVIDFLRSLGVNAYLGQKHDIRVGDRKISGNASYAFGNRVMHHGTLLFSSDLEFLAEA
                     IRVEPGLFTGSAIQSNRSRVANTVDFLSPKMDIEQFTNRMMEWFAWKVPNGLPYQLSD
                     ADTLAVDLLVKEKYSTWGWNYGFSPSFERICPLTTSLGAVTMRISVAHGHVESTAVDV
                     VPDERLAAWCKTFLARLQGKRYDIQSVADALVGAVEEYPGLEPGVTLDELLTCLIG"
     gene            complement(6087..6626)
                     /locus_tag="PK839_09780"
     CDS             complement(6087..6626)
                     /locus_tag="PK839_09780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG73063.1"
                     /translation="MRYILLAVFCLISSFAYPQGVAQDTIILVSNKVIVGKVQNVSSS
                     KITLIKKDSQVPEEITRKQIHKIKYSNGRVEQFNSMAFSMVEESNFQAVIITDKPEDV
                     DGLYAYGKIESASGRTSKSAKAAEKNARIRLQRKAAAMGAMYVLITKSETRGGYKEVP
                     THFYEGIAYGIEPPKEQPK"
     gene            complement(6782..7123)
                     /locus_tag="PK839_09785"
     CDS             complement(6782..7123)
                     /locus_tag="PK839_09785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG73064.1"
                     /translation="MKPLKVAQPLASWLVRIIFAFYLFLIYLKGINPVQLNSLNFYLS
                     LTMLVFSVLLVVGGFLKTQTLTVVSAVIVGLILLYKALAPAPTAINAEFAQQALLISI
                     AFLFVCKGNQP"
BASE COUNT         1920 a         1460 c         1679 g         2087 t
ORIGIN      
        1 tacaaggggc tgcgcccctt gctacttatt acgccccttc ggggcttaaa ccaccagaca
       61 agttccgcca gtaagcggac aagttccgcc ggtaggcgga caagttctgg aatttgaaat
      121 ccgccggtag gcggacaggt tctgaaatct gaaatttgaa atccgtcggt agccggacag
      181 ttaccgtaat tttaaattag ccaattccgc ccaatacttc attcaacatt tattttttca
      241 ttcttaaaca ggcttaagat tagtagctat cgtcccaagg gatacctcta cctaaactac
      301 ctttatgtat ttcattttta tgttgagttt tcttaacatt tttttaaatt gccacccaat
      361 tatttttcag aacttactat agaaagtgca ggatgagcta taagtgtgtt ttggttgacg
      421 atgagccgct ggccgttggt gtcctggaag gcttcttaag gcacattcct gacattgaag
      481 ttgtagccac ttttaacgag gcaataccac tttttgactt cattcagcat cacaaggttg
      541 acatcatttt tctcgatatt gaaatgccca acctttcggg aatagacttc ctaaaatgcc
      601 taaaggatcc tccttttgtc ataattacct ccgcgaaccg cgactatgcc gttgatggtt
      661 acgagcttaa cgttgttgac tacattctta aaccccttac catggagcga ttggtaaggg
      721 cggttagtag agctgctgag gccctggaat tgaaggctag gaatgtaacg gctgctccag
      781 atgcagagta tatcttttta aaggagggta aaaaattcat tcgggttaaa gttgatgaaa
      841 ttcaatacat tgaaagtatt aaggattatg tgaaagtggt agctgaagga aaaacagtag
      901 ttacacgaat gaatattagt gcggttgaaa agttgcttga tcctgccgag tttataaggg
      961 tgcatcggtc atttattatt tcaaagaaac atattgacgc ctacacagca acctctatcg
     1021 aaattggttc ggttgaaatt cctgttggaa ggatttatcg ggatgaagca gtcaggcggc
     1081 ttggctatga ttttgaagtt tagcaagctg ccaggcgatg gcttttgatg taccgcgctt
     1141 ggcattgcca tttgttggct ttagccctac accagagttc tttccgcaag ggaggggtgc
     1201 gccttggggt ttaaatacct agatttgggg agaggggaac ccataatgat gttgtcgggg
     1261 agcgaatccc tactatcaac ccaaaaaaat gttatgagta gccgttttac tttacacttt
     1321 aacgttacca tgagcattta cacccggtat attctggcag tcgttttggc actcattggt
     1381 tatcctaaaa tgccgcaagc gcaggagaga ggcctgtttt tatcgaggta ctttccagca
     1441 agcgagtaca atggctcaac gcagaattgg tgtgctgttg aggacaacag gggggtgatg
     1501 tattttgcaa atggtcaggg cgttctcgag tatgacggtg caacttggaa tctgattagg
     1561 gttgataacg aatctgcaat acggagcctg gcagccgatt cctccggcag gatatacgtg
     1621 ggagcctttg gagaaatggg ttatctttac cccaaccgga gtggccagct gcggtatatg
     1681 tcgctaatgt ccttgctgga taaaaatcat tcgaggttta gccagatttg gaatatctac
     1741 acttttgggg atacagcatt tttcctttcc gacagctgtc tgtacaggta ctgtaatggc
     1801 aagttcagct actggactag ccaggaaggt tgcttctatt ttgcttttaa attcaacaac
     1861 cacctttaca ttcaggaaac tggagttggg cttcagcaat ttgatgctga ttctcttgtg
     1921 cttgttgaag agggcaatct gcttgcatcg caacggatac acgctatttt accctttggg
     1981 aacgaaatgc tggtttgcac acggagtaat ggcttgtttc tctttagaaa tgaaggggta
     2041 aaaaatcggc tcaccccagt ctcgcaccta tcgggcagag ctttagcagt tgaccgttat
     2101 tttaaggagt actcattcta ccatggagtg gcaataaacg ataatctcct cgccttgagc
     2161 tccattaatg gcaatttact tatggttgac acaacatggc tggttaggga ggttattgat
     2221 tacaggacaa agggagtaaa gagccccgtt aactacctgt acctccaaaa aaacaacctg
     2281 ctctggttgg ctctcgacaa tggaatttgt gtggttgaga ttaattcgcc atttcgctac
     2341 tggactgatg acatgggggt taacggtgtg gttaccgatg tagctgagct tggcggtttt
     2401 ctgtatgtct caacccaagt tggaaatttt tttcttgatg aaaaaaagga agaagatttt
     2461 atgcccgaca tgttcaaaaa ggttgaaggt gattttgagc agtcgtggga atacctttac
     2521 tttaaggttt ctagcaattt attccctcag ggtcatagcc gcccagctga cacccttttg
     2581 cttgtggcaa cccgaacggg tgtatatcga attaatggca gccggtcgag cagaatatca
     2641 aggtatgaac cggttttctg ctttcatcaa agtaaagcaa atcctgctgc tctcctggtg
     2701 ggcctttcta gtgggatagc tattgttgag taccgtgatg gattttgggt agataaaggg
     2761 tttgtgcctg ggattaagga gaatattgag cagatagccg aggatttaga tggaaacgta
     2821 tggttgaagg ctagctttag cggagtttac aggcttaaaa accttttctt tggcagtagc
     2881 tttaagggta gcttggtggt tgaaaagtat gatacctcga gtggtttgcc cgacctgaaa
     2941 tccttataca tgactagcca tgcagattca ctctttttct actcccaaaa cgggtactac
     3001 acctttaacg agaggttgag gcaatttcaa gcgtacaatc cggctaacaa ccggctattt
     3061 tccgccgaga atgttgatac tctggcttgg agaagggtat ggaataatat catttctggc
     3121 ttctatgttg ctcacacctc cgattccatc atatggttta acaccccaaa ggggttattt
     3181 cggtacactc caagcagcta cacccgttgt tttgatgctc ctgctacttt tatacgaaag
     3241 gtagtaaacc tggattccac gctattctat ggaaccaact ttgaaagttc gaaagtggac
     3301 agtgctgccg gaaaaccagc ttttttacct agccataatc cccttgttga tctggggaca
     3361 gtgttggagt ataaacgtaa ctctctgata ttcaactacg catcaccata tttcgaggga
     3421 gacgaccccg tgcagttctg ctatcagcta gttggttacg ataagctctg gtcggcttgg
     3481 acaaccagta accgcaagga atataccaac ctaccagagg gcttctacac ttttaaagtt
     3541 aaggctaaaa attacatggg aattgaaggc gctcctgccg aatttcggtt tagagttaaa
     3601 cctccttggt accgaactgc cttggcatac tttggctata tgattatagc ttttctactc
     3661 attgccggaa tcattcgggc atatacatac catctggttc ttgaaaagaa taagctggag
     3721 cagctggttg ctgaaagaac ccaggaaatt ttgcagcaga aggaggaaat tcaggtgcag
     3781 gccgaacacc tcaagagtgc taacgaaaga attttgctaa agaatgctga gcttgaagcc
     3841 cggaagaatg aaattgaaaa tcagaaaaac aagctggagg aaagctacac tacaatgaat
     3901 accttttttg gtgtgattgc tcacgattta cggaacccta taagtatcct ggtaaacttc
     3961 atggggattt tactttccga ttttgacagc atggatagaa aaaaagttgt agcttatatc
     4021 gaggagttga acaagctggc tctcaacacg tacaacctgt tggagaacct gctcgattgg
     4081 tcgaaaagcc aaatgggcag catcagctgt aatcttaagc cagttaacct taaatcgctg
     4141 gtgattcaga atgttgagct ggtgcagggt aaaattgaac aaaaaaaggt taacctgaga
     4201 gtgatgattc ctgaagagtg catcgtttca gccgatgaaa atatgctgca cactattgtt
     4261 cggaacttga tatcgaatgc cttgaagtac acctacgaca acggggaaat attgatttac
     4321 gccgaactta gaaactccac atgctacctg agcgtgaagg ataatggggt tggtataagc
     4381 agcgagctta aagggaagct attcaaagcc gatagagatg caacaactcc gggtctccac
     4441 aacgaaaagg gttcggggct tgggctgata ctctgcaaaa cgtttgtaga acaaatgggc
     4501 ggaaacatcc tggtggagag ccaaccggga ctaggaagca cgtttaccgt tacattaaaa
     4561 ctagagggaa tacctggcta gccgctcctc aatactaccc atttaaatgg tattgaatgc
     4621 tgccgcaatt ccctcggcca tggcattaac gccctcatcg agcttcttac ccagcaacat
     4681 tttcatcatc atgttgagtt cagcctttag ggtaattttc attcgggtat cgtagggagc
     4741 aacctccttt agctgaatcc aaagataaaa ttgcatgggg aggttatcat caccggtaat
     4801 cttgatggtt ttattttttt ccttttccac aatctttagc cctacaggcc cttttccctg
     4861 gtatttaaag ctacatcttt gctcctcggc ctgccattcc tcaacccttt gggcaaccat
     4921 tggagtaaaa tttctaaagt ccgaaagcat tccgtaaatg tcctcagcgc gcttggagat
     4981 ggcaacaatt ttactttcgt atgtggtcat tttttgccta aatttgtcga tgcaaattaa
     5041 gtgaaaattt aagtattccg gttagccccc ggtgcttact ccatggttaa gtttattctt
     5101 cagcaaagcc taaatcccta ctttaacatt gcctcggagg aatacctgct gcgcagcttt
     5161 agcgacgatt ttatctacat ctaccgatgc aacccctcga tagttgttgg taagcaccaa
     5221 aatacgtttg ccgaggtcaa cattcggttg gtggagcggc tctccattcc tgtggttaga
     5281 aggctatcgg gtggaggaac cgtgtttcac gatttaggca acattaactt ttgctttatt
     5341 tcgaatgggg tccctgggca gctggtagat tttaaacgtt ttaccttacc ggttatcgat
     5401 tttcttcgta gcctcggggt taatgcctat ttgggccaga agcacgacat tcgggtgggc
     5461 gataggaaaa tatcgggcaa tgcaagctat gcttttggta atcgggtaat gcatcacgga
     5521 actctcctgt tttcatccga tttggagttt cttgccgaag ctattagggt tgaaccgggg
     5581 ctttttaccg gtagtgccat tcaatcgaac agaagcagag ttgccaatac ggttgatttt
     5641 ctttcgccga aaatggatat cgagcagttt actaacagga tgatggaatg gtttgcttgg
     5701 aaagtaccca atggtttacc ctaccagctg tcagatgccg ataccctggc ggtggatcta
     5761 ctggtgaaag agaagtatag tacctggggc tggaactatg gattttcgcc atcattcgag
     5821 cgtatttgtc ctctgacaac atctttgggg gctgttacaa tgcgtattag tgtagcccat
     5881 gggcatgttg agagcaccgc tgttgatgtt gttcctgatg agcggctggc agcctggtgt
     5941 aaaacctttt tggccaggct gcaagggaaa aggtatgaca ttcagtcagt agcagatgcc
     6001 cttgtgggag cagtagaaga gtacccaggc ttggagccag gtgtaaccct ggatgaactt
     6061 ttaacctgtt tgattggcta agtaaattac ttgggttgct cctttggagg ctcaatccca
     6121 tatgctatac cctcgtagaa atgagtggga acttctttat agcccccgcg tgtttcgctt
     6181 ttagtaatca gcacatacat ggcgcccatt gcggcagctt tacgctgaag tctaatgcgg
     6241 gcattttttt cggccgcttt tgccgattta ctcgtccgtc ccgatgccga ttcaatttta
     6301 ccgtaggcat ataagccatc aacgtcctcg ggtttgtcag tgataattac agcctgaaag
     6361 ttggattcct caaccataga aaaggccatg gagttgaact gctccacacg gccgttgcta
     6421 tacttgattt tgtgaatctg cttacgggtg atttcctccg gaacttgtga atcctttttg
     6481 attaaggtga tttttgaaga gcttacattt tgcaccttac caacaatcac cttattgctt
     6541 acaagtataa tggtatcctg agcaactccc tgggggtaag caaatgatga aatcaggcaa
     6601 aaaacagcaa gcaatatgta cctcatgaaa cttatcatgc taagaatttt acaaatgtag
     6661 tgccatttgc aaaaatacat ttataaataa tctaaaaatg aagtttgaat ttaccgatac
     6721 aacaggtaat tactgaaagt ttgattttac tttacaactt caacattttt aaatttttgg
     6781 gttagggttg attccccttg catacaaaca ggaatgcaat tgaaattagc aaggcctgtt
     6841 gggcaaattc ggcgtttatt gctgtgggag caggcgctag tgctttatat agaagtataa
     6901 gccctactat tacggctgaa actactgtaa gggtttgggt cttaaggaat ccacccacta
     6961 ccagcagaac agaaaacaca agcatggtaa gcgataggta aaaattcagg ctattcagct
     7021 gcaccggatt tattcctttt aggtaaatta ggaaaaggta gaaggcgaaa attatacgaa
     7081 ccagccagct tgctaggggt tgcgcaactt ttaacggttt catggtcgga tgatttaaat
     7141 gttaag
//
LOCUS       DAOSHM010000088         7061 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_9274_length_7061_cov_20.955752, whole genome shotgun sequence.
ACCESSION   DAOSHM010000088 DAOSHM010000000
VERSION     DAOSHM010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 7061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7061
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9274_length_7061_cov_20.955752"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(122..1069)
                     /locus_tag="PK839_09790"
     CDS             complement(122..1069)
                     /locus_tag="PK839_09790"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOG73065.1"
                     /translation="MKINFLNRQQVDEARWNACMDASVNGMIYGYSWYLDIVSPGWCA
                     LADEDYHYIFPLPRLKRNFNEIIIQPLYTQQLGLFSDSHLSPEILDEFICAIPSRYRY
                     IGINLNTLNRTERYADNTSARVTYRLDLFSSYENIQKKYCQNTRRNNLKAYALGCRVE
                     RSITPAEFLGFYSKYGVPRQRMRHYVKVRQLVESLMKRDRGELVGVYTPGSQLCAVSL
                     ICKSNGKLIYLLAASSPLGRQNRAMFALVDQVIRWYSETELVFDFEGSMIPSIARFYG
                     GFGATPCSYSSITINRLPLYARFLFIAENGIRTLARKVL"
     gene            complement(1059..2378)
                     /locus_tag="PK839_09795"
     CDS             complement(1059..2378)
                     /locus_tag="PK839_09795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOG73066.1"
                     /translation="MELLIYTKNNSSRLTYIARLIFNDILAIKFRITTSYEEAAAYHG
                     PLLAYNKEPVNGAIQIVPHGLLNEKGISPQNFEVLSWRNLPVFPVTSPNGDFPFDIFT
                     ASFYLVSRYEEQLPATLDEHKRYRSEQSIAVKNNFLELPVIDLWAFQLLDMLQSRFNG
                     VAGCSRKFSTMFTFDLDSAYAYRYKGFVRSALGITRSLLNLKWKELQSRARVLSGAIN
                     DPNDVYAEVARKLKGFDGTTWFIHAGSWGQHDKPISLLHPAMKKIVADLSKEFKLGIH
                     PSYKSFRKNDTLSKEINRLSGVVGMKITRSRQHYLRFRLPETYIELTKMGIHEEYSMG
                     YPDRIGFRAGTCTPFTFYDLASDETLNLKVYPFAVMDSTLIFTNNCSIGESIQTINGL
                     IDVVKSVKGLFISVWHVDYLSESYPGKGWWKVLDAVISRLKEAKGEN"
     gene            complement(2405..3688)
                     /locus_tag="PK839_09800"
     CDS             complement(2405..3688)
                     /locus_tag="PK839_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
                     /protein_id="HOG73067.1"
                     /translation="MDSITAVNRKALPPIFIIGALFFVFGFVTWLNGILIPYFKISCE
                     LTDFEATLVAFAFYISYTIMALPSSWILKRTGFHKGMTLGLLVMSVGTLLFVPAALSR
                     AYWMFLSGLFVMGAGLAILQTAANPYIAILGPRESAARRISIMGICNKLAGAVAPLIL
                     AYYILQDGDTFVQSLSGLDEVARMAALDSLAARVIGPYLVMTGVLVVLAIFVRYSPLP
                     EVEAEPEEVEADVAELAQKKNILQFPHLVVGAIALFFYVGVEVIAGDTIIRYGISQGI
                     AISTAKAFTSYTLFAMIVGYLLGIALMPKIISQRTALTISASLGLLFSLGVILSRGST
                     SIVFLALLGFANALIWPTIWPLAIHNLGRFLKTGSAILIMAIAGGAIIPLIWGKLSDL
                     YSQTPQVAYMVLIPSYLIILYYSVWGYKLRSWRRR"
     gene            complement(3794..5224)
                     /locus_tag="PK839_09805"
     CDS             complement(3794..5224)
                     /locus_tag="PK839_09805"
                     /inference="COORDINATES: protein motif:HMM:NF012432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HOG73068.1"
                     /translation="MRIETDYLGPMPIPDDALYGIHSQRAKENFPDITPFHKEWYQAM
                     GLVKQACYLTYVDFAKAVKNEPELSERLIDLIPDDILHAMISAAVEIQNGSYFEHFIV
                     PAFQGGAGTSINMNVNEIIANVALLKVGRKPGDYQLIDPIEHANIYQSTNDVVPTALR
                     VAATRLLTQLEEAINGTRQVVENLEKSYRNSLRIGYTQMQAAVPSSFGQLFSTYSEAL
                     SRDWWRVSKCFERIKQVNLGGGAIGTGIGIPRFFIMEATRHLQSLTRFPFARAENLND
                     ATANLDAFVEVHATLKAHAVNLEKMLSDIRLLASDVGRNSISIPKRQVGSSIMPGKVN
                     PVITEFTVSIAHRVYSNDMLISDLAGQGVLDLNAYIPTIGHALLDSLKLLVAANHSLS
                     NFLLDGLQVDVDKTEAQLLHNPSVTTALLPIIGFNKAAILANEMRQKDIDIFEANRVC
                     QLLPDETLREAVKPENLLRLGYSLKD"
     gene            complement(5372..6454)
                     /locus_tag="PK839_09810"
     CDS             complement(5372..6454)
                     /locus_tag="PK839_09810"
                     /inference="COORDINATES: protein motif:HMM:NF016952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminyl-peptide cyclotransferase"
                     /protein_id="HOG73069.1"
                     /translation="MKAIEFATILTILAGCTNGQKNQPRVVNSHPPIADLRLIRIEEP
                     QDGAIFPLGDNIHISIKQTGNVAPDSVVLSVNGARVERLGGLTYELSTGGLKMGTQSI
                     SLAAWKSGSRQIAGVSVKLKSNFKPEKLTYRVVRSYPHDGRAYTQGLFYHDGYLYEGT
                     GQEGESSLRKVELETGKVLRSINLGREYFGEGIALLKGMIYQLTWTSGIGFIYDFNTF
                     NNLGSFTYATQGWGLTTNGKELIMSNGSNIISFMEPSGFSEVSRIEVFDDVGPVKMLN
                     ELEYINGKIYANVYLTDRIVIINAETGAVTADVDLKGILSPSDKTGSEDVLNGIAYDF
                     QGNRLFVTGKYWPKLFEVKLVPVGKR"
     gene            complement(6552..6971)
                     /locus_tag="PK839_09815"
     CDS             complement(6552..6971)
                     /locus_tag="PK839_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007564004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG73070.1"
                     /translation="MFRIAVDFDGTIVENAYPAIGKPITFAFETLRELQKRGFAIILW
                     TVRSGKLLDEAVEFCRQNGVEFYAVNRNYPEESFDGEQPRKIEADVFIDDRNLGGFPG
                     WGKAFEMLCPEELSHELEKKALSRYKKKGFLNRLLGH"
BASE COUNT         1884 a         1598 c         1664 g         1915 t
ORIGIN      
        1 cggattctcc cttcggcagg gatggagaaa ctataatcta ctcacggttc acgaccttca
       61 tcttttcctg attcgccctt ttttcaactt gaatggtgtt atttttaatg ctgaaatagg
      121 actacagcac ctttcttgcc agggttctaa tgccattttc tgctataaaa aggaacctgg
      181 catatagggg caggcggtta attgtgatac tggagtagct gcaaggagta gcgccaaaac
      241 caccgtaaaa acgtgcaatt gaaggtatca tggaaccctc aaaatcgaat accagttcag
      301 tttctgagta ccacctgatt acctgatcaa ccagggcaaa cattgcccta ttttgcctgc
      361 ccaatggtga tgaggcagca aggaggtaga ttagcttgcc attgctttta cagatgagcg
      421 ataccgcaca tagctggctt ccgggggtgt aaactccgac caattcaccc ctatctcttt
      481 tcataaggct ctcaaccagc tgcctcacct taacgtagtg cctcatacgc tggcggggaa
      541 ctccgtattt tgagtagaat cctaggaatt cagctggtgt aatgcttctc tctactctac
      601 aacctaaggc gtaagctttc aggttgttcc tgcgggtatt ctggcagtac ttcttttgaa
      661 tgttttcgta ggacgagaac aggtcgagtc tgtaggtaac ccgtgcgctg gtattatcgg
      721 catacctttc cgttcggtta agcgtgttca ggttaatgcc gatgtaccta tacctgctgg
      781 gaatggcgca aataaactca tcgagtattt cgggtgacag gtggctatcc gaaaacagtc
      841 caagctgctg ggtgtagagc ggctggataa ttatttcgtt gaagttgcgc ttcaggcggg
      901 gcaacgggaa aatatagtgg tagtcttcat cagccaaagc gcaccagccg ggcgagacga
      961 tgtcgaggta ccagctgtag ccataaatca tcccgtttac cgaggcatcc atgcaggcat
     1021 tccagcgtgc ctcgtcaacc tgctgcctgt ttaaaaagtt aattttcacc tttagcttcc
     1081 tttaatcggc tgattactgc atccaaaacc ttccaccatc ctttgccggg gtaggattcg
     1141 gaaaggtaat caacatgcca aacgctgatg aacaaaccct ttaccgactt tacaacgtca
     1201 atcaacccat ttatagtctg tatggattca cctatggagc aattgtttgt aaaaattaag
     1261 gtggaatcca taaccgcaaa tggataaact ttgaggttta gggtttcatc gcttgccaaa
     1321 tcgtaaaagg taaacggggt gcatgtcccg gctctaaagc ctatcctatc gggatacccc
     1381 atagagtatt cctcatgaat acccattttt gtaagttcaa tgtaggtttc gggcagtcta
     1441 aatctcaggt agtgctgccg gctgcgggta attttcattc ctacaacccc cgacaacctg
     1501 ttgatttcct tgctgagcgt gtcatttttc ctgaatgatt tgtatgatgg atgaatgcca
     1561 agtttgaatt ccttcgacaa atctgcaaca atttttttca tggcaggatg taacaggctg
     1621 atgggcttgt cgtgctgtcc ccaacttcca gcatggataa accaggtggt accatcaaaa
     1681 ccttttagct tcctggcaac ttcggcgtac acatcatttg gatcgttaat ggctccgctt
     1741 agtactcttg cccggctttg cagctccttc catttgaggt ttagcagcga gcgggtgata
     1801 cccaaggcag acctaacgaa ccctttgtac cgataggcat aggcgctatc caaatcgaag
     1861 gtgaacattg tgctgaactt acgggaacaa cccgctacac cattaaacct tgattgcagc
     1921 atatcaagta gctgaaatgc ccacaaatca ataacgggga gctccaaaaa gttgttttta
     1981 acggcaatgc tctgctctga gcggtagcgt ttatgctcat ccaacgtagc cggcagctgc
     2041 tcctcataac ggctaaccag gtagaacgag gctgtaaaaa tatcaaatgg gaaatcgcca
     2101 ttaggtgagg ttacagggaa aacaggtaag tttctccatg atagaacttc aaaattttgg
     2161 ggtgatatcc ccttttcgtt gagcaatcca tgtggaacaa tttggatagc cccatttaca
     2221 ggctctttat tgtaggctaa cagtgggcca tggtaggccg cagcttcttc gtacgaggta
     2281 gtgatacgaa actttatggc cagaatatca ttgaagatta accgggcaat gtaggtcagg
     2341 cgcgaggaat tatttttggt atatataagt agttccacta caggtagtta caggtgatat
     2401 ggttttacct tctacgccat gatcgtagtt tataccccca aacggagtag tagaggatga
     2461 taaggtaaga tggaataagt accatgtagg caacttgagg ggtttgggaa tacaggtcgc
     2521 tgagtttacc ccagataagc gggatgatgg ctccgccggc aatggccatg ataagaattg
     2581 ccgagccggt tttaaggaat cggcccaggt tgtgaatggc aagcggccaa attgttggcc
     2641 aaatcagggc gttggcaaag ccaagcaggg ccagaaacac gattgatgta ctgccccttg
     2701 acaaaatcac cccaaggcta aaaagcaacc ccagcgatgc tgagatggta agggctgtcc
     2761 gctgggagat aatttttggc atgagtgcta tacccagcag gtagcctacg atcatggcga
     2821 atagagtgta ggaggtaaat gcttttgcgg ttgatattgc aataccctgc gaaattccgt
     2881 agcgaataat agtatcgccg gctattactt caacgcctac gtagaagaat agtgctatag
     2941 cgccaacaac cagatgggga aactgcagga tgtttttctt ttgggccaat tcggcaacgt
     3001 cggcttccac ttcttcgggt tcagcttcta cctcggggag aggggagtac cgaacgaata
     3061 tggccagaac aactagcact ccggtcataa ccaggtaggg gccaataacc cttgccgcaa
     3121 ggctatcaag tgctgccata cgggcaactt cgtcgagccc gctaagcgac tgcacaaatg
     3181 tgtcaccatc ctgaagaatg tagtaggcca ggataagtgg ggcaactgcg ccggccagct
     3241 tgttgcatat acccatgatg cttatccgtc gggctgcact ttcacgaggt cccagtatgg
     3301 ctatgtaggg atttgctgca gtttgcagaa tggccaaccc ggcgcccatt acaaaaagcc
     3361 cggaaaggaa catccagtaa gctctactta acgctgctgg aacaaaaagt aacgtaccta
     3421 ccgacattac cagcagccct aaggtcattc ccttatgaaa acccgttcgc ttgagaatcc
     3481 atgaggaggg aagggccatt atggtgtagg atatgtagaa ggcaaaagct accagcgtag
     3541 cctcaaaatc agtcagctca cacgaaattt tgaagtatgg gattagtatt ccgttaagcc
     3601 atgttacaaa tccaaaaaca aagaatagcg caccaatgat aaatatgggt ggtaaagcct
     3661 ttcggttaac agctgttatt gagtccatag aagttcggtt aggtttctca aaaatattaa
     3721 atggttgaaa ttttaagcaa tagccttcga aataatttag ggaattaatg cgattaataa
     3781 ccggaataac ggcctagtct ttaagtgagt aacccaaacg gagtaaattc tctggtttaa
     3841 ccgcttccct aagagtttcg tcgggtagaa gctggcaaac cctgttggct tcaaagatgt
     3901 caatgtcctt ctgcctcatt tcgttggcga gtatggcagc cttattgaag cctataatgg
     3961 gtaagagtgc agtagttacc gacgggttat gcagcagctg tgcctcggtt ttatcaacat
     4021 caacctgaag cccatctaac agaaagttgc ttagggagtg gtttgcggca accagcagct
     4081 taagggagtc gagaagtgca tgtcctatag tgggaatata ggcgttgagg tcaagaacac
     4141 cctgacctgc aagatctgaa atcagcatat cattcgaata aaccctatgg gcaatgctaa
     4201 cggtaaactc cgtgataacc ggattcacct taccgggcat gatggagctg ccaacctgac
     4261 gcttggggat tgatatgctg tttctgccaa catcggaggc aagtagccta atgtcggata
     4321 gcattttctc gaggttaacg gcatgggctt taagggtggc atgaacctca acaaaggcat
     4381 cgaggttagc ggtggcatcg tttaaatttt cggctcttgc aaagggaaaa cgggtgaggg
     4441 attgaagatg ccgggtagct tccatgataa aaaaccgcgg gatgcctata ccggttccaa
     4501 tggctcctcc gccaaggtta acctgcttta ttcgctcaaa acatttggac acccgccacc
     4561 agtcgcgtga aagcgcttcg ctgtatgtgc taaaaagctg gccaaatgat gaaggaactg
     4621 ctgcctgcat ttgggtgtat cctatgcgaa gcgagttgcg gtaacttttt tcaagatttt
     4681 cgacaacctg acgggtgccg tttattgctt cctcgagctg ggtgagcagc ctggttgctg
     4741 ctacccgtaa agcggttgga actacatcgt tggttgattg gtagatgttg gcatgttcaa
     4801 tgggatcgat aagctggtaa tcaccaggct ttctaccaac ttttagaagg gcaacgttgg
     4861 caataatctc gttcacattc atgtttatgc tggtgcctgc accaccctga aatgcgggaa
     4921 caatgaaatg ctcaaagtag ctgccgtttt gtatttctac ggctgcagaa atcattgcat
     4981 gtaggatatc atcggggata aggtcaataa gcctttcgga aagttccggt tcgttcttaa
     5041 cggctttggc aaaatcaaca taggttaagt aacaggcctg cttcacaagc cccattgcct
     5101 ggtaccattc cttatggaat ggcgtaatat cgggaaaatt ctcctttgcc cgctgcgagt
     5161 gtattccata tagggcatcg tcgggaattg gcattgggcc aaggtaatct gtttctatgc
     5221 gcatctacct gaatgttaga tttttttgat ggttacatgg attctgcctg taaaaaaaat
     5281 tagtacgcat aaaccatctt aaatgctgaa atcaaaaaaa ataatttaga tgaaataata
     5341 gtagctaaaa gaatgtggct taaagctgct gctagcgttt tccaaccgga acaagcttca
     5401 cctcgaaaag ttttggccag tacttacccg taacgaatag tcgatttcct tgaaaatcgt
     5461 aggcgattcc atttagcacg tcttcacttc cggttttgtc cgacgggctg agaatcccct
     5521 taagatcaac gtcggctgta actgcgcctg tttcggcgtt aataataact atcctatcgg
     5581 taaggtaaac gttggcgtaa atttttccat taatatactc cagctcgttg agcattttta
     5641 caggtcctac atcgtcgaaa acctcaattc tactcacttc cgaaaatccc gaaggttcca
     5701 tgaatgagat gatgtttgag ccgttgctca tgattagctc tttaccgttg gttgttaacc
     5761 cccatccctg ggtggcatag gtaaatgagc cgaggttatt aaacgtatta aaatcgtaaa
     5821 taaaaccaat accgctagtc catgtaagct ggtaaatcat gccctttagt agggctattc
     5881 cctcaccaaa gtactccctg ccaaggttga tacttcttaa caccttgccg gtttccagct
     5941 caaccttacg caagctcgat tctccttcct gtcctgtacc ctcgtaaaga tacccatcgt
     6001 ggtaaaatag accctgagtg taagccctgc cgtcgtgggg gtaagagcga accaccctgt
     6061 aggttagctt ctcgggttta aagttcgact tcagcttaac ggaaacacca gctatttggc
     6121 gtgagccgct cttccatgca gcgagtgata tgctttgtgt ccccatcttt aagccaccgg
     6181 ttgaaagttc ataggttaaa cctcccaacc gttcaaccct agcgccgttg accgatagca
     6241 ccaccgaatc gggggcaaca ttgccggttt gtttgatgga gatatggata ttatcgccca
     6301 ggggaaatat agcaccatcc tggggttctt caatccggat aagccggagg tctgctatgg
     6361 gcgggtggct gtttactacc cgtggctgat tcttttggcc attggtgcat ccagccaaaa
     6421 tagtgagaat agtggcaaat tcgatcgctt tcatgaactt atacatttta gctgtcggga
     6481 aattatgtcg ttatgaagtg atttgcttta ccgtatgcca aactgttagg tgttaatctg
     6541 gttaacttgg gttaatgtcc gagtaacctg tttagaaagc ctttcttttt gtatcggctt
     6601 aatgcttttt tttcaagctc atggcttaat tcttccgggc aaagcatctc aaaagcctta
     6661 ccccatccag gaaatccacc aaggtttcta tcatcaataa acacatcggc ctcaattttg
     6721 cggggctgct cgccgtcaaa cgattcttcg gggtagttac ggtttaccgc ataaaattcc
     6781 acaccgtttt gcctacaaaa ttccacggct tcatcgagga gtttacctga acgcactgtc
     6841 cagagtatga tggcaaagcc tcttttttgc aactcgcgta gagtttcaaa ggcaaaagta
     6901 atgggcttac caatagctgg gtaggcgttc tccacaattg ttccgtcaaa gtcaactgca
     6961 atcctgaaca tttcaattcg tttgacaggc caaatctacg ttaaatttag aaaagaagat
     7021 gcccggcact tagaaaatca aacctgttga agcgacagct a
//
LOCUS       DAOSHM010000089         7022 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_9338_length_7022_cov_19.934836, whole genome shotgun sequence.
ACCESSION   DAOSHM010000089 DAOSHM010000000
VERSION     DAOSHM010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 7022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7022
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9338_length_7022_cov_19.934836"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2867)
                     /locus_tag="PK839_09820"
     CDS             complement(<1..2867)
                     /locus_tag="PK839_09820"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOG73071.1"
                     /translation="MKRILSIFTAVILLSSAVFAQQKVIPLKGSKSAISVKQSTLQQL
                     RVSVSFSSLISNEVVTESGEKYVNLAIPDGFSTGLVGTPKLPAYKKLIQVPLGAKPIV
                     KVHSFSHVDIALGDYGINHPVIPVQPPVRKDQNLDSLKFIMKAEVYRKNEMIEPQLVK
                     VDMLGTMRGVQVARLEVNPVAYNPVTGMLRIYNDIDVEVDFEGASQAKFDEMLAKTSS
                     PYFETSYSFLINDLTKSSFDDHPDLTTYPVKMLIVSNRMFESTLVPFVEWKTRKGFNV
                     EVAYTDVIGNTAEAIKTFVHSSYTNATPENPAPTFLILVGDVAQVPASGVGSASYKQT
                     DLYYASVDGDYFPEMYYGRMSASTNVQLQNIIDKILYYEQYGFEDPTYLNSATLIAGA
                     DATWNPKVGQPTIKYGTKYYFNNAQGYSSIYEYGVSNDPSNPGAQQDCIGCYGSDQVA
                     VGFINYTAHCSETSWADPTLTSSTVEAMTNLNRYPLAVGNCCLSGDFATDECIGEKWI
                     RAAGKGAVTYIGSSPSTYWFEDFYWSVGAFPMVGENDGYVPTFEETTLGAYDAPHHGN
                     YVTTGGMVFAGNLAVTEVDAQGYPQQSSPHYYWEAYNILGDPSLVPYFTEASPNVVSH
                     TDSFPIGLSLYTVSALPGSYVAISKDGLLHGAALVDASGEVDVPVNPVLEGGDVDIVV
                     TRPQTIPYMARIPAAALDGPYITLDLYTIEDDNAKADFGEKFTIAVTLKNVGVDIANT
                     VSARLSGVDRYVTITDGDSVYFGDIPSGETGNTSTVNAAFELAVADSIPDRYVATFNL
                     DATDGTSTWPSKLRITVNSPKLSVKDFTLVGDDNSNGQLDPGENGQLRVLLSNQGHAD
                     AGDALVILSDDSPFLNINNPSKIVSLPLNSDLPVDFDVSASSGTFVGTEVNVTAAVSK
                     GGYTAEGNYHFGIGDKPEVRIGTGNLAAEGTYPFDSYYKANR"
     gene            3101..4291
                     /locus_tag="PK839_09825"
     CDS             3101..4291
                     /locus_tag="PK839_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlmU family protein"
                     /protein_id="HOG73072.1"
                     /translation="MAAGFILFDDDRREALLPFTFTRPVSEIRIGILTITQKWERYLG
                     SSTSFLTTGYLSKKYPPNITGERFCLINGGVCPDKALVNAIMSLEAGEVLVHENVVLA
                     GVMDKLPAETPSLSDFRVRIPYPNEVLQVKHLWDIFRLNGEALTRDFDMLTLNRKSAP
                     ISSSNRTLNPSRIFIEEGARVECSTLNAEAGPIYIASDAEVMEGALIRGPFSLGEHSA
                     VKMGAKIYGPTTIGPHCKVGGEINNSVIFGYSNKAHDGFLGNSVIGEWCNIGADTNNS
                     NLKNNYAEVKLWSYADNAFTSTGLQFCGLFMGDHSKCGINTMFNTGTVVGVCANIYGS
                     GFPRNFIPCFSWGGACGFKTHQPEKALETARLMMQRRNLELTQTDIDILKKIFDLTSQ
                     YRNF"
     gene            4395..6869
                     /gene="lon"
                     /locus_tag="PK839_09830"
     CDS             4395..6869
                     /gene="lon"
                     /locus_tag="PK839_09830"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005640969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HOG73073.1"
                     /translation="MERNSRNFYSKLLIDDSNESNVNFIPILADDGTPELKDADIPDV
                     LPIMALRDTVLFPGIVMPITVGREKSLKLIKSLPPRKRMIGVVAQVDSKIPDPTAEDL
                     WHIGTVAQVIKVLEMPDRNFSVILKGICRFEVKNYLSEEPFFMARVSTLADILPDESD
                     KDFAAIASSIRDITIRIINLTPTLPQEAAFAIKNLDDNQLLVNFVSANSDISPEEKQK
                     LLEKSMLKERATQLLQYLTREVQMLELKQDIQSKVKLDLEQQQREYFLQQQIKTLQEE
                     LGANPTEQEVNDLKERAKQKKWSKEIAEHFDRELSKLNRMNPMAAEYSVQLNYVELLL
                     DLPWNEFTQDNFDLAHAREILDEDHFGLEQVKERILEHLAVLKLKGDLKAPILCLYGP
                     PGVGKTSLGKSVARALGRKYVRISLGGMHDEAEIRGHRKTYIGAMPGRIIQSIKKVNS
                     SNPVIILDEIDKVGQDFHGDPASALLEALDPEQNFAFHDNFLEVEFDLSKVLFITTAN
                     NIATISPALRDRMEMIEVSGYCMEEKVQIAKRHLVPNQITEHGLNSSQLQLNEKTIEE
                     IIKNYTRESGVRNLNQRIAKVVRNVAKKVAFEETYNPRLTIKEVRSILGVPRYNSDSS
                     IGNELAGVVTGLAWTETGGEILFVETSLSKGKGNLTLTGNLGDVMKESAVLALEYIKA
                     HANLLGINPDIFERWNVHVHVPEGAIPKDGPSAGITMVTSLASAFTQRKVKSSIAMTG
                     EITLRGKVLPVGGIKEKILAAKRANIMDIILPFENKKDVLEINSDYIKGVKFHYVGNI
                     MEALTFALLPEKVTNPICLEEPPRNN"
BASE COUNT         2036 a         1648 c         1623 g         1715 t
ORIGIN      
        1 cggtttgcct tgtagtagct atcgaatgga taggttcctt ctgccgccaa gttgccagtg
       61 cctatcctca cctcgggctt atcgccaatg ccaaagtgat aattcccttc agcagtgtaa
      121 ccccccttag aaaccgcagc tgttacgtta acctcggttc caacaaacgt acctgaactg
      181 gctgatacgt cgaaatcaac cggtaaatcg gaattgagag gtaacgaaac gatttttgaa
      241 ggattgttga tatttaagaa aggagaatca tccgagagga ttaccagggc atcaccagca
      301 tcggcgtgac cctggttcga tagtaatacc ctaagttgcc cattttctcc cggatcgagc
      361 tggccgttgg agttgtcatc gcccactagg gtaaaatctt ttaccgacaa cttgggtgaa
      421 ttcaccgtaa tcctcagctt agatggccac gtgctggtcc cgtcggtagc atcaaggtta
      481 aaagttgcca catacctatc gggaatgctg tcggccacgg ctagctcaaa cgctgcgttt
      541 accgtcgaag tatttccggt ctcacccgaa gggatatcgc caaagtaaac cgagtcgcca
      601 tccgttatcg taacatacct atctacacct gaaagccttg ccgatacggt attggcgatg
      661 tcaacaccaa cattttttaa ggttacggca atcgtaaact tttcaccgaa atcagccttg
      721 gcattatcgt cctcaatggt gtataagtca agcgtgatgt agggcccgtc caaagcggca
      781 gcgggtatcc tcgccatgta gggaatggtt tgtggtcggg taaccacaat atccacatcg
      841 ccaccctcca gaacgggatt aaccggaaca tcaacctcac ctgaagcgtc aaccagggca
      901 gcgccatgta gcaaaccatc ctttgaaatg gcaacatagg aacccggaag tgcgctgaca
      961 gtgtaaagag acagtccaat aggaaagcta tcggtatgcg aaactacatt tgggctagct
     1021 tcggtaaagt aaggcacaag tgaaggatca cctagtatgt tgtatgcttc ccagtagtaa
     1081 tgcggactcg attgctgtgg atatccctgg gcatcaacct cggttacggc caggttaccg
     1141 gcaaacacca ttccaccggt agtaacgtaa ttgccatgat ggggcgcatc gtaggcgcca
     1201 agggtagttt cctcgaaggt aggaacatac ccatcatttt cgcccaccat gggaaatgca
     1261 ccaacagacc agtagaaatc ttcaaaccaa tatgttgagg gagaagaacc aatgtaggta
     1321 acagctcctt taccggcagc tctaatccat ttctcgccaa tacactcatc ggttgcaaag
     1381 tcgcctgata ggcagcagtt tccaacagcc agggggtacc tgtttagatt ggtcattgct
     1441 tcaacagttg aagatgtaag agttggatcg gcccatgagg tctcgctgca atgggcggtg
     1501 tagttaataa acccaacagc aacctgatcg gaaccatagc aaccgatgca atcctgttgg
     1561 gcacctggat tacttggatc gttagaaacg ccatactcgt aaatgcttga gtatccctga
     1621 gcattattga agtagtactt ggttccgtac tttatggttg gctgaccaac cttcggattc
     1681 caagtggcat cggcaccggc aatcagggtg gccgaattaa gataggttgg atcctcaaac
     1741 ccgtactgct cgtagtaaag tatcttatct ataatattct gaagctgaac atttgtacta
     1801 gccgacatgc gcccgtagta catctcggga aagtagtcgc catcaacgct tgcatagtaa
     1861 aggtcggttt gcttgtaaga ggcggaaccc acgcccgagg cgggaacctg ggccacgtcg
     1921 cccacaagga taagaaatgt tggtgcaggg ttttcgggag tagcgtttgt gtaggaacta
     1981 tggacgaagg ttttgattgc ttcggccgtg ttaccaatga catcggtata ggccacctca
     2041 acgttgaacc ctttcctcgt tttccactca acaaagggga caagggttga ttcaaacatc
     2101 ctgttcgaaa caattagcat cttaaccgga taggtggtca agtcgggatg atcgtcaaag
     2161 gaacttttgg taaggtcgtt gattagaaag ctgtaggatg tctcaaagta tggtgaagaa
     2221 gttttggcaa gcatttcatc aaacttagcc tggctagccc cctcaaagtc aacctcaaca
     2281 tcaatatcgt tgtatattct aagcataccg gttaccggat tataggccac agggttcact
     2341 tcaagccttg ccacctgaac tccacgcatg gttcccagca tatcaacctt aaccagctga
     2401 ggttctatca tctcgttctt acggtaaacc tcagccttca tgatgaattt taatgagtca
     2461 agattctgat ccttacgaac aggaggctga acgggaataa caggatggtt aattccgtaa
     2521 tcacctagcg caatatcaac atggctgaaa ctgtgaacct ttactattgg cttggcccct
     2581 agtggaactt gaatgagctt cttgtaggca ggcagtttgg gagtgccaac aagtccggtc
     2641 gaaaacccat ctggaatagc caggttaaca tacttttcgc ccgattccgt taccacttca
     2701 tttgatataa gcgagctgaa actaaccgaa acccttagct gctgaagagt gctctgctta
     2761 acgcttatgg ccgatttact accctttaag ggaataactt tttgctgggc aaaaacagca
     2821 gaactcaaga gaattacagc ggtaaaaatg gatagtatcc ttttcatcac aggaaaaata
     2881 ttgaatcaac cataacaaca aacattgtgc aagttaagct attatgttaa aaaatgccaa
     2941 tttagagctt tcatgtcctg cctaaaaaag ttttacaaat tagcaactag gggaatcaag
     3001 cagttcagcc aagaccggca gcttggtaaa attcatagta gccagaaaac ctctaacaag
     3061 cgaccgattt tttaactacg tttgtcttaa atttaaggtt atggcagctg gttttatcct
     3121 atttgatgat gatagaaggg aagcccttct ccccttcacg tttacccggc cggtttcaga
     3181 aattcgaatt ggcattctaa caattaccca aaaatgggag cgttaccttg gtagttcaac
     3241 atcatttctt accacaggct acctttcgaa aaagtatcca ccaaacatca ctggtgaaag
     3301 attttgcttg ataaacgggg gtgtttgccc ggataaagcg ctggttaatg ccataatgtc
     3361 attagaagca ggcgaagtgc tagttcatga aaatgttgtg ctagcaggtg taatggataa
     3421 actacctgcc gaaacaccat ccctgtcgga ttttcgtgtc agaattccct accccaacga
     3481 agtgcttcag gtgaaacatc tctgggatat tttccggcta aacggtgaag ccttgaccag
     3541 agacttcgac atgctaacat taaacagaaa atcggcacca atttcgtcaa gtaaccggac
     3601 tcttaaccca tccaggatat ttattgagga gggtgcaagg gttgaatgct caactctaaa
     3661 tgccgaagcc ggccccatat atatcgcctc ggatgccgag gttatggagg gagcccttat
     3721 caggggtcca ttttcgcttg gcgagcattc cgccgtgaag atgggtgcta aaatttacgg
     3781 ccccaccact attggaccgc actgtaaggt tggaggtgaa ataaacaaca gcgtaatttt
     3841 tggttactca aacaaggccc acgatggctt tctggggaac tcggtgattg gcgaatggtg
     3901 taacattggt gccgacacca acaactccaa cctgaaaaac aactacgcag aggttaaact
     3961 ctggagttat gcggataacg catttaccag caccggactt caattctgcg ggttgttcat
     4021 gggcgaccat tccaaatgtg gcatcaacac catgtttaac accggaacgg tggtgggtgt
     4081 ctgcgccaat atttatggca gcggatttcc ccgaaatttt attccctgct tctcatgggg
     4141 tggggcgtgt ggatttaaaa cgcatcaacc tgaaaaagct ttggaaactg cccgtttaat
     4201 gatgcagcgt cgaaatttgg agctaaccca aactgacatc gacattctaa agaaaatttt
     4261 tgacctgaca agccagtacc gaaactttta agtgcaacca tgtaaaactt tttggcatag
     4321 gtattgccca tatcaatact cgaaaaaata aatcgagata atgacatgtt gtcattaggc
     4381 attgaggtat cagtatggaa agaaatagca gaaatttcta tagcaagctt cttattgatg
     4441 actccaacga gtcaaatgtg aattttatac ccattctcgc cgacgatggt acacccgaac
     4501 tcaaggatgc tgatattccg gatgttttgc cgattatggc gcttcgcgat acggttctct
     4561 ttccgggtat tgttatgcct ataacggttg gcagggaaaa atcgctaaaa ctaataaaaa
     4621 gcctaccccc gcgtaaaagg atgattggag tggttgccca ggttgactcc aaaattcccg
     4681 acccaactgc agaagatttg tggcatattg gaaccgttgc ccaagttatt aaagtgctcg
     4741 aaatgcccga taggaatttt tcggtaatac taaagggaat ttgccgattt gaagttaaga
     4801 actacctcag cgaagaaccc ttttttatgg ccagggtaag cacgcttgca gacattctcc
     4861 cagatgaaag cgacaaggat tttgctgcaa ttgccagctc tattcgcgat ataaccatcc
     4921 ggatcattaa cctcaccccc accctacccc aagaggcagc ctttgccatt aaaaacctgg
     4981 acgacaatca gctacttgtc aactttgtaa gcgcaaacag cgacatcagc cctgaagaaa
     5041 agcaaaagct acttgaaaaa tccatgctaa aggagcgagc cacgcagctc ctccagtacc
     5101 ttacacgcga ggttcaaatg ctggagttaa aacaggacat acagtcgaaa gtaaagctcg
     5161 atcttgagca gcagcaacgg gagtacttcc tacagcagca gattaaaacc ctgcaggagg
     5221 aacttggggc caaccccaca gaacaggaag taaacgatct gaaagaaagg gcaaagcaga
     5281 aaaaatggag caaggagata gccgagcatt tcgaccggga actcagcaag ctaaaccgga
     5341 tgaaccccat ggctgctgag tactccgttc agctcaacta cgtggagctg ctcctcgatc
     5401 tgccatggaa cgagtttact caggataact ttgaccttgc gcacgccaga gaaattctgg
     5461 atgaggacca ttttggactc gaacaggtta aggagcgcat tcttgaacac ctggccgttt
     5521 tgaagctgaa gggcgacctt aaagccccaa tactctgcct gtatggccct ccgggagttg
     5581 gaaaaacatc tcttggaaaa tcggttgcac gagcactagg cagaaagtac gtgcgcattt
     5641 cacttggggg tatgcacgac gaggctgaaa ttagggggca tcgtaaaacc tacatcggag
     5701 ccatgccggg caggataata cagagcatta aaaaggttaa ctcatccaat ccggttatta
     5761 ttcttgatga gattgacaag gtgggacagg attttcatgg cgatcccgca tctgcccttt
     5821 tggaagcgct cgatccggag cagaacttcg catttcacga caacttcctt gaggtcgaat
     5881 tcgacctatc caaggtgctg ttcatcacaa ctgccaataa catagccacc ataagtcctg
     5941 ccctgcgtga ccgaatggaa atgattgagg ttagcggcta ctgcatggag gaaaaggttc
     6001 aaattgccaa acgccacctg gttcccaacc agataaccga gcatggtctc aactcctccc
     6061 agctgcagct aaacgaaaaa acaattgagg aaataataaa aaactacacc cgagaaagtg
     6121 gcgtacgcaa ccttaaccaa agaattgcca aggttgtgcg aaacgttgcc aaaaaggtgg
     6181 cgtttgaaga aacatacaat ccccgcttaa ccataaagga agtaaggtct attctgggtg
     6241 ttccgcggta caacagcgac tcatccattg gaaacgagtt ggccggagta gtaaccggac
     6301 ttgcctggac cgaaaccggt ggcgaaatcc tttttgtaga aacaagcctt agcaagggga
     6361 aaggaaatct tacccttacc ggaaatctgg gtgatgtgat gaaggagtcg gccgttctgg
     6421 cactagagta cataaaggct catgccaacc tgctgggaat aaaccctgat atttttgaaa
     6481 ggtggaatgt gcatgttcac gtacccgaag gtgccattcc caaggatgga ccatctgctg
     6541 gaataaccat ggttacctcc ctggcatcag ccttcaccca gcgtaaggtt aaaagcagca
     6601 tagccatgac cggtgagatt actcttcgtg gcaaggtgct gccagttgga ggcataaagg
     6661 aaaaaatcct ggctgccaaa agggctaata tcatggatat aatcctcccc ttcgaaaata
     6721 aaaaggacgt tctggagata aacagcgact acattaaagg ggttaagttc cactacgtag
     6781 gcaacataat ggaagcattg acatttgcac tgcttcccga aaaagttaca aaccctattt
     6841 gtctggagga gccccctcgt aataattaag cataaagaaa cctcttccca agcaacaatt
     6901 taaagccctt agaagacgga ataattttgg cgaatccacc aactctgcat cgggtttcac
     6961 caacagtcgg acaagtctac cttatccttt aaccttttct ccagccctaa cgggagaaaa
     7021 gg
//
LOCUS       DAOSHM010000090         6972 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_9426_length_6972_cov_20.381524, whole genome shotgun sequence.
ACCESSION   DAOSHM010000090 DAOSHM010000000
VERSION     DAOSHM010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 6972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6972
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9426_length_6972_cov_20.381524"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1511)
                     /locus_tag="PK839_09835"
     CDS             complement(<1..1511)
                     /locus_tag="PK839_09835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG73074.1"
                     /translation="MRKLGFILLFFCLLCSAGYSQTTYHHISNFGVYEFIDELASLKV
                     VELYSTVKPYSRAQIAEALKQADAKRSELNRRQQGELDFYLADFGKELGSFPKRKRFD
                     LLYRKDSLFSITLNPLLGVDLISNDSALAYHRWVGAEVHGHVAGWGYYASLRDYAENY
                     RLAMPTYLTRQQGGNYKYSGKGGEFSEMRGGVTYSWRWGNVGVVKDNIQWGSGYNGSN
                     ILGGRTPSFAMIKLNITPARWVELNYLHGWLVSEVIDSSRTYTSGDEVRKVYHPKYIA
                     ANLIAFKPFRGLSLSVGNSIIYSDINVQPAYLIPFLFYKSVDHHLNGMSNSVGQNSQM
                     FFDISSRNIRHLHLYATLFLDEISMKNMFNPDMHSNFYSLKTGVRISDFPFSNLIVTG
                     EYTRTNPLTYRHIINTTTYESNNFNLGHYLQDNADEIYVSLVFRPIRGLQVSMSYSVS
                     RKGPDYSTITTTRLGLPFMESVEWELREWAVGAKYEVINDGYIYLSGGVSDIT"
     gene            complement(1679..>2530)
                     /locus_tag="PK839_09840"
     CDS             complement(1679..>2530)
                     /locus_tag="PK839_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013760947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FumA C-terminus/TtdB family hydratase beta
                     subunit"
                     /protein_id="HOG73075.1"
                     /translation="VKGNEFGQAFRDLELEEKIFRAANSIGLGAQFGGKYLALDVRII
                     RLPRHGASCPVGIGVSCVADRNVKAKINRHGIWVEKLENNPGRFIPEQWRKPDEEGAV
                     GIDLNQPMETIRAELSKHPVGTRLSLSGKIVVARDIAHARLKERMDRGEGLPQYLKNH
                     PVYYAGPAKTPEGMPSGSFGPTTAGRMDSYVDAFQSQGASLVMIAKGNRSAAVTEACK
                     KYGGFYLGSIGGPAAILAHENIKKVELLEYPELGMEAVYMIEVSDFPAFILVDDKGND
                     FYKQVAR"
     assembly_gap    2531..2630
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2631..2962)
                     /locus_tag="PK839_09845"
     CDS             complement(<2631..2962)
                     /locus_tag="PK839_09845"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016278137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HOG73076.1"
                     /translation="MAVPEFYYQERFPLGEEETEYYLLTRDFVSLSQFEGKDILRVDP
                     QGLELLARTAMHDTSFMLRTGHVRQLAAILNDPEASKNDKFVALVLLRNAEIAAKGIL
                     PLCQDTGTA"
     gene            complement(2980..3840)
                     /locus_tag="PK839_09850"
     CDS             complement(2980..3840)
                     /locus_tag="PK839_09850"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HOG73077.1"
                     /translation="MKKKFVLIFLASVLMSGFLPAQNENELSISQLENEVHRLVNAHR
                     KSIGLGELAPNSYIRKEAYQHSLNMAEGKVPFSHKGFEERFERLSGFLDITSGAENVA
                     NGYNSAQDIVDGWLLSPGHKKNIEGDFNLTGIGIAQAPNGTYFYTQIFVKSQERPKIV
                     ASDFESELLGLINEHRKGLGLSALKSNDVVKAEAVNYSGQMAAGRAPIGPPGYDNPMK
                     ALLSKMNAVAMTEIIAYNYYTAKEVFNSWMASTRQRETIEGSFDLTGIGVVQSADGKV
                     FVTQIFLLSR"
     gene            complement(3858..4202)
                     /locus_tag="PK839_09855"
     CDS             complement(3858..4202)
                     /locus_tag="PK839_09855"
                     /inference="COORDINATES: protein motif:HMM:NF015893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
                     /protein_id="HOG73078.1"
                     /translation="MLKIYHNPRCAKSRKGLEYLSSRGIEFEVKNYMKEGLRADEVRE
                     IFLKSNIKPSQLARTQDDYYKMYLKGKNFSDDEWVQIYVDNPAILQRPIVVGRYKAVL
                     AIPPENMDVLFK"
     gene            4344..>4942
                     /locus_tag="PK839_09860"
     CDS             4344..>4942
                     /locus_tag="PK839_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008621956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG73079.1"
                     /translation="MIKPFKTLLFLLSVFIVFLGISFFFPSGKKNIAKITLYVPSTDS
                     LLGAITSKPAMAEQPEANKKPPIMLNAEVDSNKTVLPTPENGEFKKSMMKTDTLTRNT
                     SLAYSTNSTATDKIVPIEFTTEALGSFFAALKNGEAGSSQVRVLHFGDSQVEGDRISD
                     YLRSVLQEKFGGYGPGILPAYPQNYQPYGIRQSASKGWN"
     assembly_gap    4943..5042
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5043..5660
                     /locus_tag="PK839_09865"
     CDS             <5043..5660
                     /locus_tag="PK839_09865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG73080.1"
                     /translation="HILIYGLSLESERGIYVDNIPLRGSSGEIFTGFDHELARQLFGM
                     LNVKLVILQFGLNVAPGNLTSYDYYEERLYKQVMAIKSFKPDVSVLIIGISDMLRRVD
                     GRLETYPSIEKIRDAQRSAAFRSGAAFWDCYRAMGGKNSMLTWVNSNPPLGSMDYAHF
                     TYRGARVIAGKLTKSLLEAFDRYSFPESSAIPTEAIARPDSSQGG"
     gene            5747..>6972
                     /locus_tag="PK839_09870"
     CDS             5747..>6972
                     /locus_tag="PK839_09870"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007480131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HOG73081.1"
                     /translation="MGSWTNILSSIFSYTEGNPLLFTEMSFWVFFAVLLTGYSFIYNR
                     PLARSAYLLAFSLYFYYKSSGLFFFLLIFSTVCDYTIGLLIPRYSSKTARKLLVALSV
                     IINLAVLSYFKYAYFITETVNQLFGLNLEVVNYLSGWANGIFGSHFDTSTIILPAGIS
                     FYTFQTISYTVDVYRRKVAPVKNIIDFGFYVSFFPQLVAGPIVRAAEFIPQLYRKFSL
                     TYREFGHALFLIIAGLVKKMVISDYLSVNFVDRVFDNPLSFSGFENLMATYGYALQIY
                     CDFSGYTDIAIGVALLLGFRLPPNFNSPYKAVSIADFWHRWHISLSTWLRDYLYIPLG
                     GSRKGKVRTHLNLMVTMLLGGLWHGANLKFVVWGGIHGVSLVLHKLWTSHSPRVKRAD
                     SWWRRAVGAFFTFQLVS"
BASE COUNT         1707 a         1748 c         1531 g         1786 t
ORIGIN      
        1 tcggtaatat ccgaaacacc gcctgaaagg tagatatagc cgtcattaat cacctcatac
       61 tttgccccta ctgcccactc ccgtagctcc cattccacgc tttccataaa tggtaaccca
      121 agcctggtgg ttgttattgt gctgtagtcg ggccccttac ggctgacgga gtagctcatg
      181 gaaacttgaa ggcctctgat aggcctaaat accagcgata cgtaaatttc atcggcattg
      241 tcctgcaggt agtgccccag gttaaagttg ttgctttcgt aagttgttgt attaattata
      301 tgcctgtacg tcaacgggtt tgtgcgtgta tattcacctg ttacgatgag gttgctaaat
      361 ggaaaatccg aaattctgac accggttttc aggctgtaga agttactgtg catatcgggg
      421 ttgaacatgt tcttcatcga aatttcatcg aggaaaaggg tggcgtacag gtgcaggtgt
      481 cttatgtttc gcgacgaaat gtcaaagaac atctgggaat tttgtcccac cgagttgctc
      541 atcccgttca ggtggtgatc aacggacttg tagaacagaa aggggatgag gtacgcaggc
      601 tgaacgttaa tatcggagta gataatggag ttgccaaccg acaggcttag ccccctgaag
      661 ggtttaaagg caattaggtt agcggcaata tacttggggt ggtacacctt tcgcacctca
      721 tcaccagagg tgtaggtgcg gctggagtcg ataacctcgg aaacaagcca cccgtgcagg
      781 tagttcagct caacccaacg tgcaggagtg atgtttagct tgatcatggc aaaggaaggt
      841 gtccgcccgc cgagaatgtt ggaaccgttg tagcccgatc cccactgaat gttgtccttt
      901 acaacgccaa cgttacccca cctccagcta taggtaaccc ctcccctcat ctcgctgaac
      961 tccccgccct tccctgaata cttataattt ccaccctgct gccgggttaa ataggtcggc
     1021 atggccaacc ggtagttctc ggcatagtca cgtaagctgg cgtagtagcc ccatccggca
     1081 acatgcccat gaacttcagc tcccacccaa cgatggtaag caagtgcgct atcgttcgaa
     1141 ataaggtcaa cacccagcag cgggtttagg gttatgctga atagcgaatc ttttcggtaa
     1201 agaaggtcga aacgcttgcg tttgggaaat gagcctagct cctttccaaa atcggcaagg
     1261 taaaaatcaa gctcgccctg ctgacgcctg ttcagctccg accttttagc atcggcttgc
     1321 ttaagcgcct cggcaatttg cgcacgagag tatggcttca ccgtgctata aagctcaacc
     1381 accttaagcg atgccagctc atcaataaat tcgtacacac caaagttgga gatatgatgg
     1441 taggtggttt gggagtatcc cgctgagcac aggagacaaa agaatagcag gataaaacca
     1501 agctttctca tttccataaa tttgaattct attactaaat tgatagccac gcggatactg
     1561 cttatagcaa gacggtaagc cgcattcatg agtgacgaaa tttcccctcc aaatttcctt
     1621 aaacaccatg ctgatatttg gccgaatggt agcagaaatc caccaagcgc catcgaaatc
     1681 acctggccac ctgcttgtaa aaatcgttgc ccttgtcatc aaccagaatg aaagcaggaa
     1741 agtcggaaac ctcaatcatg taaactgcct ccattccaag ctcggggtac tccagcagct
     1801 ccaccttttt aatattctcg tgcgcaagaa tggcagcagg tccgcctatg cttcccaggt
     1861 aaaagcctcc atatttcttg caggcctcag taactgcggc gctcctgtta cccttggcaa
     1921 tcatcacgag gcttgctccc tgcgactgga atgcatcaac ataggagtcc atccggccgg
     1981 cagtggtggg gccgaaagag cccgatggca taccctctgg ggttttagcg ggaccggcgt
     2041 agtaaaccgg atgattctta aggtactgag gcagcccctc ccccctatcc atacgctcct
     2101 taagtcgggc atgggcaata tcgcgggcca caacaatttt gccgcttaac gaaagtcgtg
     2161 tgccaaccgg gtgcttcgat agctcggctc gaatagtctc cattggctga ttcaggtcta
     2221 taccaacagc gccttcctca tcaggtttac gccactgctc ggggataaac cttcccgggt
     2281 tgttttccaa cttttcaacc cagataccgt ggcggtttat cttggccttt acattcctat
     2341 cggccacgca tgataccccg attcctaccg gacaggatgc gccatggcgg ggaagcctga
     2401 taatcctaac atccagggca aggtacttgc ccccgaactg tgcaccaagt cctatactgt
     2461 tggctgcacg gaaaattttc tcctcaagct ccaaatccct gaaagcctgc ccaaactcat
     2521 tccccttaac nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gcagttcccg
     2641 tatcctgaca aaggggcagt atgcccttag cggctatttc ggcattccta agcagaacaa
     2701 gggcaacaaa cttgtcattc ttactagcct cggggtcgtt aagaatagct gccagctgcc
     2761 tgacgtgccc ggtgcgaagc atgaacgatg tgtcgtgcat ggctgttctg gccaacagct
     2821 ccaggccctg cgggtcaacc cttagaatat ccttaccctc aaattgcgaa agggaaacaa
     2881 aatccctggt tagaaggtag tactcggtct cctcctctcc caaaggaaac ctttcctggt
     2941 agtaaaattc aggaacagcc attttggttg aattttatgt taacgactaa gtagaaaaat
     3001 ctgcgtaaca aaaaccttcc catcggcaga ctgcacaact ccaatgccgg taaggtcgaa
     3061 gcttccctct atggtctctc gttgcctggt gctggccatc cagctgttaa acacctcctt
     3121 ggcagtgtag tagttatagg caattatttc ggtcatggca acagcattca tctttgaaag
     3181 caacgccttc atggggttat cgtaccccgg aggacctata ggcgctctac cggctgccat
     3241 ttgcccggag tagttcaccg cttcggcctt aaccacatcg ttgcttttca gtgcgctaag
     3301 ccctaatcct ttacgatgct catttatcag ccctaatagt tctgactcaa agtctgatgc
     3361 aacaatttta gggcgttcct gacttttgac aaatatctgg gtgtagaagt aggtgccgtt
     3421 gggcgcctgg gcaatcccaa ttccggttag gttaaaatca ccctcaatat tttttttatg
     3481 cccggggctt aacaaccagc catcgactat gtcctgagcc gagttgtagc cgttggcaac
     3541 attttcggcc ccggaagtaa tgtccaaaaa accgcttagc cgttcaaatc gctcttcaaa
     3601 acccttatgg ctaaagggaa ccttaccttc ggccatgttc aacgagtgct ggtaggcctc
     3661 ctttcgaata tagctattag gagccagctc tcccagccca atagattttc tatgcgcatt
     3721 taccaggcgg tgaacctcgt tctccagctg tgaaatgctc aactcatttt cgttctgagc
     3781 tggcaaaaat ccgctcataa ggacggaggc aagaaaaatc aagacaaatt tcttcttcat
     3841 caggacaaac tatttattta tttaaagagt acatccatat tttcaggagg aatagccagc
     3901 acggctttat acctgcccac cacgatgggt cgttggagaa tggcggggtt atcaacataa
     3961 atttgaaccc attcatcatc ggaaaagttt ttccccttca aatacatctt gtagtaatca
     4021 tcctgagttc gggctagctg gcttggttta atattcgact ttaggaatat ctctcgaacc
     4081 tcatctgccc ggagcccctc cttcatatag tttttaacct caaactctat ccccctgcta
     4141 ctcaaatact ctaaaccctt tctgctcttt gcacatcgag ggttatggta tattttcagc
     4201 ataaaatatt ttttttgaaa gttacaaaaa aatggcgatg gttttacccg ctataattct
     4261 tatatgtgaa attagcaatt ttacaaaatt accttacttt tatacgggta ttacagaaaa
     4321 aatgccacta aaagctacga taaatgatta agccttttaa aacccttttg tttctgctat
     4381 cggttttcat cgtgtttcta ggaatttcgt ttttcttccc aagcggaaag aaaaacattg
     4441 ccaaaatcac cctgtatgtt ccttccaccg acagcctgct tggggccatc acctcaaaac
     4501 cagcaatggc agaacaacca gaagcaaaca agaaaccacc cataatgctc aatgctgaag
     4561 tagattcaaa taaaaccgtg ctacccaccc ctgaaaatgg cgaattcaaa aaatcaatga
     4621 tgaaaaccga cacgttgaca aggaatacgt cgttggcata ctccacaaac agcactgcta
     4681 ccgacaaaat tgttcctatt gaatttacta ccgaggccct gggtagcttc ttcgccgctt
     4741 taaaaaatgg ggaggccggc agctcccagg ttagggttct acactttggc gactcacagg
     4801 ttgagggcga tcggatttct gactatttac gttcggtgtt acaggaaaag tttggtggtt
     4861 acggcccagg cattcttcct gcctacccac aaaactacca accttacgga atcaggcaat
     4921 cggcgtcaaa agggtggaat tannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5041 nncatatcct gatatatggt ctatcgcttg aatcggagcg gggtatttac gttgacaata
     5101 ttcccctaag gggaagctcg ggcgaaattt ttacgggatt cgaccatgag ctggccaggc
     5161 agctgttcgg tatgcttaac gtgaagctgg ttatcctgca attcggtttg aatgtagccc
     5221 cgggcaacct aaccagctac gactactacg aagagcgtct ctacaagcag gtaatggcca
     5281 tcaaatcgtt taaacccgat gtttccgttc tcatcatcgg catttcggat atgctgcgca
     5341 gggttgatgg ccggctcgaa acctatccca gcatcgagaa gatcagggat gctcaacgga
     5401 gtgcggcttt caggtcgggt gcggctttct gggattgcta ccgggcaatg ggagggaaaa
     5461 actccatgct cacatgggtc aattccaatc ctccgcttgg gtcaatggat tacgctcact
     5521 tcacctacag gggggccagg gtgattgccg ggaaacttac aaaatcgctg cttgaagcct
     5581 ttgataggta tagtttcccc gaaagctccg ctataccaac cgaagcaatt gccagacccg
     5641 acagtagcca gggtggttga gctgaataat ctgtcagcat tttgccataa gaaaaaaatc
     5701 accacgggtg gaagatatca aaaacgatta gacctaaaag aaaagaatgg gatcatggac
     5761 caacatactg tcaagcatct tcagctacac tgagggcaat cccctgctat ttaccgagat
     5821 gtcgttctgg gtgttttttg ccgtactgct tacgggttat tctttcatct acaaccggcc
     5881 gttggcccgc agcgcatacc tgcttgcgtt cagcctttac ttctactaca aatcgagtgg
     5941 attgtttttc ttcctgctca tcttctcaac agtatgcgac tacaccattg ggctactcat
     6001 tccccgctac agctcaaaaa ccgcccgtaa gctgctggtg gcgctaagcg ttatcatcaa
     6061 ccttgccgtg ctctcctact tcaagtatgc ctacttcatc accgaaacgg ttaaccagct
     6121 gtttggctta aaccttgaag tggtcaacta cctttcgggg tgggcaaacg gaatttttgg
     6181 ttcccacttc gacacctcaa ccatcatcct gccagccggc atttcgttct acacattcca
     6241 aaccatcagc tacacggttg acgtttaccg ccgcaaggtg gctcctgtta agaacatcat
     6301 tgactttggc ttttatgttt cgttctttcc ccagctggtg gccggcccca ttgtgagggc
     6361 agccgagttt atcccccagc tctacagaaa gtttagcctg acctacaggg agtttggtca
     6421 tgcgcttttc ctaattattg cagggttggt gaaaaaaatg gtcatatcgg actacctttc
     6481 ggtcaacttt gtggataggg ttttcgataa cccgcttagc ttctcgggat ttgaaaatct
     6541 gatggccacc tacggctatg ccctgcagat atactgcgat ttctcgggat acaccgatat
     6601 tgccatcggg gttgcccttt tactaggctt cagacttccg ccaaacttca actcgcccta
     6661 taaagcagtc agcattgccg acttctggca taggtggcac atctcgctgt ccacatggct
     6721 tcgcgactac ctctacattc cgcttggcgg tagccgtaag ggaaaggtca gaacacacct
     6781 caacctgatg gttaccatgc tgctgggtgg gttatggcat ggtgccaacc tgaagtttgt
     6841 ggtttggggc ggcatccacg gggttagcct tgttctgcat aagctttgga cttcacattc
     6901 gcccagagtc aaacgtgctg acagctggtg gagaagggcg gttggcgcat tttttacctt
     6961 ccagctggta tc
//
LOCUS       DAOSHM010000091         6711 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_9926_length_6711_cov_4.689303, whole genome shotgun sequence.
ACCESSION   DAOSHM010000091 DAOSHM010000000
VERSION     DAOSHM010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 6711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6711
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9926_length_6711_cov_4.689303"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            348..4658
                     /gene="cas9"
                     /locus_tag="PK839_09875"
     CDS             348..4658
                     /gene="cas9"
                     /locus_tag="PK839_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005791619.1"
                     /note="Cas9, originally named Csn1, is the large,
                     multifunctional signature protein of type II CRISPR/Cas
                     systems. It is well known even to general audiences
                     because its RNA-guided endonuclease activity has made it a
                     popular tool for custom editing of eukaryotic genomes;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR RNA-guided endonuclease Cas9"
                     /protein_id="HOG73082.1"
                     /translation="MKKILGLDLGTSSIGWALVNEAENEGEKSEIIKLGVRVNPLTVD
                     ELLNFEKGKSITTNADRTLKRSMRRNLQRYKLRRENLISILKDNNLITHESILTENGN
                     RTTFETYRLRAKAATEEITLEQFARVLLMINKKRGYKSSRKAKGSEEGTLIDGMEVAK
                     ILYNEHLTPGQYCLQQIKNGKKYLPDFYRSDLQQEFDKIFEKQMTFYPELLTKDLQRE
                     LRGKKRDAVWAICSKSFKEKGFELVGIKRQGNSAEQKLENFVWRDKGLSEKLDLEQLV
                     VVLQQINIQVNSSSGYLGSISDRSKELFFNQIAKSDGSTRAQTVGEYQMAILKNNPNA
                     SLRNMVFYRQDYLDEFNTIWEKQAEYHKELTEELKREIRDVVIFYQRPLKSQKSLISF
                     CEFESRKVEITVDGKKKIRTIGSRVIPRSSPLFQEFKIWQILNNIEVYGKGRKNRKQQ
                     EPSLLNISEDPLKNSGKRKLYQEEKELLAAELTIIKELKKADVLKLLFDNHKELDMNF
                     NSIQGNSTQAALFDAYKQIIELSGHEVDYEKKSANEIIKAVEEIFSGLGFNTDILYFN
                     SDKPLDEQPMYKLWHLLYSFEGDSSKTGNENLINKIKELYGFDRESAKILANVTFQDD
                     YGSLSARAIYKILPRMKQGEDYATACAYIYNKHSKNSLTKEELEKKVYKDKLEILPKN
                     SLRNPVVEKILNQMINVINSIIDNYGKPDEIRIELARELKKSAKEREELTKSIAETTK
                     LHEQYKSILSKPPFNLQYISHNDIVRYKLYEELKENGYKTLYSNTYISPTTVFSGDFD
                     VEHIIPQSRLFDDSFSNKTLELKSINIEKGNKTAYDFIKEKYGETGSENCLDVYLIRI
                     EDLRKKGVISKTKYNKLKMQEKDIPDGFINRDLANSQYIAKYAKTMLGELVKFVVSTT
                     GSITDRLREDWQLVDVMKELNWEKYKSLGLTEEITNGEGQRIRKIKDWTKRNDHRHHA
                     MDALTVAFTKRQYIQYLNNLNARRVSDYQGISNAEKEEHDNFSIAAEDVILNTRDVLG
                     IEKKYLYRDDKNKLRFKPPIPLDEFRAEAKHQLENTLISIKAKNKVTTRNINITKKKG
                     GVNKKEQLTPRGQLHNETVYGSHKEYVVKEETVSAKFNSEKIALVANPEYRKALAERL
                     QQFGNDPKKAFTGSNALSKKPIWLNELHTKQVPEKVKTVSFETIYTIRKEVSPDLKVD
                     KVVDKKIRQKLETRLKGFEGDAKKAFSSLEENPIWLNEEKGISIKRVTITGVNNVESL
                     HEKRDKYGNYVLDKNGNRQPVDFVSTSNNHHVAIYRDAEGNLQENVVSFFEAVNRRNL
                     GLPVIDKNYKSGDGWKFLFSMKQNEYFVFPNEKTGFNPNEIDLLNPDNYSLISPNLFR
                     VQKMATKDYTLRHHLETNVETNNQLKGITWIRTGLNGLLNIVKVRINHLGQIVSVGEY
                     "
     gene            5090..6026
                     /gene="cas1"
                     /locus_tag="PK839_09880"
                     /pseudo
     CDS             5090..6026
                     /gene="cas1"
                     /locus_tag="PK839_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004369788.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR-associated endonuclease Cas1"
     gene            6034..6372
                     /gene="cas2"
                     /locus_tag="PK839_09885"
     CDS             6034..6372
                     /gene="cas2"
                     /locus_tag="PK839_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004360001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HOG73083.1"
                     /translation="MTFERLNAYRIMWILVLYDLPTETRAQRKASARFRKNLLGSGFS
                     MFQFSAYIRHVASSESAEVQKRRVKSLMPEMGAIGILEITDKQFEKMEIYYGRKTQKT
                     PKGPVQLELF"
BASE COUNT         2362 a         1310 c         1386 g         1653 t
ORIGIN      
        1 gagcagcaga tcgggctggt ggtttacctc aggaaggcag atagggtgta aaaaagccgc
       61 caagggactg taacaaagca taatggaaat gaaaacggaa tgataggcgg cttgtcgttt
      121 tgtcaaatac tggaactaat acaatgaccc ctttaaaaac acacctgata aagtatagtg
      181 ctgaagggaa atgtatggag ctactgacgg taaatactaa aaaaggcgat gcttagcacc
      241 gccttaaatg ttttcacaac ggaataatcc ttattgtgat ttgcttacaa tttgattaca
      301 agtataaact atatttttta actttccaaa aattttaaca ctattttatg aaaaaaatat
      361 taggacttga tttaggcacc agttcaattg gttgggcctt agtaaatgag gcagagaatg
      421 aaggcgaaaa atccgagatt attaaacttg gagtacgcgt aaatccttta acagtagatg
      481 aactgctgaa ttttgagaaa gggaaaagca ttaccacaaa tgccgataga accttaaaaa
      541 gaagtatgcg ccgcaaccta caacggtata agctacggag ggaaaacttg ataagtattc
      601 taaaggataa caacctcatc acccatgaat ccattttaac ggaaaacggt aaccggacca
      661 cgttcgaaac atatcgcctt agggcaaaag cggcaacaga agaaataaca cttgagcaat
      721 ttgcccgggt cttactaatg atcaacaaga aacggggtta taaaagcagt agaaaggcca
      781 aaggaagtga agaaggaacc ttaattgacg gtatggaggt ggctaaaata ctttataatg
      841 agcatttgac cccaggccag tattgcctac aacaaattaa aaatgggaaa aagtatctgc
      901 cagattttta tcgttctgac ctgcagcagg aatttgataa gatttttgag aaacaaatga
      961 ctttttatcc tgaattgctg actaaagatt tacagaggga gcttcgtggc aaaaaacggg
     1021 atgccgtatg ggcaatctgt tcaaagtcat ttaaggaaaa gggattcgaa ctagttggca
     1081 ttaaacggca aggaaattca gctgagcaga aactcgaaaa ttttgtttgg agagacaaag
     1141 gattatccga aaagttggat ttagagcagc tggtggtcgt gctccagcaa attaacattc
     1201 aagttaatag ttctagtggt tacttgggtt ctattagcga tcgtagcaaa gagctattct
     1261 tcaaccaaat agctaaatca gatggtagta ccagggcaca gactgtcggt gagtatcaaa
     1321 tggcaatctt aaaaaataat cccaacgcaa gcttgcggaa tatggtgttt taccggcagg
     1381 attatttgga tgaatttaat acgatttggg aaaaacaggc agagtatcac aaagaactaa
     1441 cagaagaatt gaaacgggaa attcgtgatg tagtgatttt ttatcaacgc cccctaaaaa
     1501 gtcagaagag cttgattagc ttttgcgagt tcgaaagccg aaaagttgag ataacagttg
     1561 acggcaaaaa gaaaatcaga actatcggca gccgtgttat tccacgctca tcaccactat
     1621 tccaagaatt taaaatatgg cagattttaa ataatattga agtttatggc aaaggcagaa
     1681 agaataggaa acaacaagaa ccatctttac ttaacatttc cgaagaccct ttaaagaatt
     1741 caggtaaaag aaagttatat caggaagaaa aggaattgct tgcagcagaa cttaccataa
     1801 taaaggagtt gaaaaaggct gatgtgctta agttgttgtt cgataatcac aaagagttgg
     1861 atatgaactt caactccatt caaggaaatt caactcaagc agcacttttc gacgcctaca
     1921 agcaaataat tgaattaagt ggacacgaag tagattatga gaaaaaatct gccaatgaaa
     1981 ttataaaagc tgttgaagaa attttcagtg gtttagggtt taatacagac attttgtatt
     2041 tcaattccga caaacccctt gatgagcaac ccatgtacaa gctatggcat ttgctatact
     2101 catttgaggg agatagctca aaaactggaa atgaaaatct tattaacaaa ataaaagaac
     2161 tttacggctt tgacagggaa tctgccaaaa ttttggctaa cgttactttt caggacgatt
     2221 acggctcttt gtcggcaagg gcaatttaca aaatcttacc acgcatgaaa cagggtgagg
     2281 attatgcaac tgcatgtgct tatatttaca acaaacactc taagaattct ctaactaaag
     2341 aagagctgga aaaaaaagtt tataaggata aacttgaaat cctacctaaa aattcgctac
     2401 gtaacccggt ggtggagaaa attctcaacc aaatgataaa tgttatcaac tctattatag
     2461 acaattatgg caaacctgat gaaattcgta ttgagttggc ccgtgaattg aaaaagagcg
     2521 caaaggaaag agaagagtta accaaatcga ttgccgaaac caccaaattg catgagcaat
     2581 ataagagtat tttgtctaaa cctcctttca accttcagta tattagccac aacgatattg
     2641 ttcgttacaa actttatgag gagcttaaag aaaatgggta taaaaccctg tactccaaca
     2701 cgtatatatc tcccactact gtttttagcg gagattttga tgttgagcac attatccctc
     2761 aatcgcgtct tttcgacgat tcattttcaa acaaaacatt agaattaaag agcattaata
     2821 tagaaaaagg taataaaaca gcatacgact ttattaagga aaaatatgga gaaaccggca
     2881 gcgaaaactg ccttgatgtt tatcttatcc gcattgaaga cctgcgcaaa aaaggtgtca
     2941 tttcaaaaac taagtacaac aagcttaaaa tgcaggaaaa ggatataccc gatggtttta
     3001 tcaaccgaga tttagcaaac tcacagtata tagccaaata tgccaaaacg atgcttggtg
     3061 agcttgtgaa atttgtagta tcaactacag gctcaattac agacagatta cgcgaggatt
     3121 ggcagctggt tgatgttatg aaagagttga actgggaaaa atataaaagt cttggattga
     3181 cagaagaaat tacaaatgga gagggacagc gaatcagaaa aattaaggat tggaccaaac
     3241 gtaacgacca taggcaccac gcaatggatg cccttacggt tgcattcact aaacgacaat
     3301 acattcagta cctcaacaat ctgaatgctc gacgagtaag cgattatcaa gggatttcaa
     3361 acgctgaaaa agaagaacac gataactttt caattgctgc agaggatgtt attctgaaca
     3421 caagagatgt gctgggtata gaaaaaaaat acttataccg ggatgataaa aataaacttc
     3481 gttttaaacc tccaataccc cttgatgagt tcagggcaga agctaagcat cagcttgaaa
     3541 atacgctaat ttccataaaa gccaaaaata aagtaacaac aaggaatatc aatattacga
     3601 aaaaaaaggg gggggtcaat aaaaaggaac agctaactcc acgtggacag ctacacaacg
     3661 aaactgttta cggcagccat aaagaatatg tagtaaaaga agaaactgtt agcgcaaagt
     3721 tcaacagtga aaaaattgct ttggtagcca atccagaata tcgtaaagcc ctagctgaac
     3781 gcttgcagca attcggtaac gaccctaaaa aagcttttac tggctcaaac gctctctcta
     3841 aaaaaccgat ttggttgaac gaacttcaca ccaaacaagt tcccgagaag gtaaaaacag
     3901 taagcttcga aactatctat actattcgca aggaggtatc acccgatttg aaggtggata
     3961 aggtggttga taaaaaaatc cgacaaaagc ttgagactcg attgaaaggg tttgaaggag
     4021 atgcaaaaaa ggcattctcc agtttggaag aaaaccccat ttggcttaat gaggaaaaag
     4081 gaatttccat aaagcgtgtt accattaccg gagttaacaa tgttgaatcg ctacatgaga
     4141 aacgggataa atacggtaat tatgttttgg ataaaaatgg taaccgacag cctgttgatt
     4201 tcgtgagcac cagcaacaac catcatgttg ccatctaccg cgatgccgaa ggcaacctgc
     4261 aggaaaacgt tgtttcattc ttcgaagccg taaacaggcg caacctcggt ttacccgtga
     4321 tagataaaaa ttataagtct ggcgacgggt ggaaatttct cttcagcatg aagcaaaacg
     4381 agtactttgt ttttccaaac gaaaaaaccg gttttaatcc caatgaaatc gatttgctca
     4441 atcccgacaa ttactccttg attagcccga acctgtttag agtgcagaaa atggctacaa
     4501 aggattacac tttaagacat catttagaaa caaatgttga aaccaataat caattgaaag
     4561 ggattacatg gattagaact ggtttaaatg gtttgctaaa cattgttaaa gtaaggatta
     4621 atcatttggg gcaaatcgtt tcggtagggg agtattagtt cagaatgttg aatttacccc
     4681 gtgccatctc taccaaacac ccatgtagct gaaccacaac cgaacatttt tcatagctct
     4741 acaaagcaaa acgcccatga gcagagcatg tgggagtttg cttatagtat aactacatga
     4801 gctccttcca aataaaattc aacataaaaa ttgttaccaa cttatagaac aagtaacgta
     4861 taaaaaattg atggacacat gccggggcat agcctgcctg gttgaaagtt tcgcaagtaa
     4921 tttagaattt agagtatgga ggaagggaag gaaacgaaaa atttacgcct ttccctaaag
     4981 gtaaagcaca attcattcaa aataagctcg ttgggtacgg gttttgctgt acctcattgt
     5041 gagtataaat tttactcgag cacagctacc ctttttataa gtacagacta tgatcaaacg
     5101 cacgctttac tttggcaacc cttcattttt aagcctccgg aacaaccaac tattgctgcg
     5161 gactcctgaa attgagaagc acgaagaggt aaaaaggctg ctcccaaaca agggcgaaat
     5221 cacttttcat cgaagatctg ggatttgttg tgatagacca cccccagatt accattacgc
     5281 aatccctgtt ggaaatgctg gttgccaaca acgttgccct ggtaacatgc gataaatcgc
     5341 acctgcccac cggactgctt ctggcactgc actccaacac cctgcaaacc gaaaggtacc
     5401 atgagcagct ggacgcctcc gagccactta aaaaacaact atgggcacag ctcatacagc
     5461 aaaaaatagc caaccaggca gcttcgctta aagccagagg ctgccaaacc gatgccgact
     5521 ttttgcaaaa gcttagccaa agcgtaaaaa gcggcgacag cgataaccgc gaggccgttg
     5581 ctgcgtcggc ctactggcct aagctttttg ccggtatccc cggcttcacc cgctcacgta
     5641 ccggtattcc gccaaacaac ctgttgaact acggttacgc cattttaagg gccacggtag
     5701 cccgcagcat cgtttgcacc gggctgctgc caacgctagg cctgcaccac cacaaccgct
     5761 acaaccctta ctgcctggcc gacgacctaa tggaacccta ccggccattc gttgacgtgc
     5821 ttacctgtag cctgaccgaa aaattcggta tggtggagga gcttggcaaa gaggtaaaaa
     5881 cagagctgct ggccattcct gcaatggata taaaattaga aaaccaaacc agcccgttga
     5941 tgattgcaac gcagaaaaca tgccagtcgc tgcaaaagtg tttttcgggt gaacagcgaa
     6001 aactttcgct gccagtccta acttgaccat accatgacgt ttgaacgatt aaatgcgtac
     6061 cgcataatgt ggatactagt tctctacgat ctacccaccg aaacaagggc acagcgaaag
     6121 gcgtcagcac gcttcagaaa gaatttactt ggaagcggat ttagcatgtt tcagttttcg
     6181 gcctatatta ggcatgtggc aagcagcgaa tcggccgaag ttcaaaagcg cagggtaaag
     6241 agcctgatgc ccgagatggg cgccatcgga atactggaaa taaccgacaa acagttcgag
     6301 aaaatggaaa tatactatgg gcgaaaaacg caaaaaacgc caaaaggtcc cgtgcagttg
     6361 gaactattct aggttcataa atgttaggta taggaataaa aaaatcccga aattcgggat
     6421 ttcttagggt tctacgttag gaattttcaa tccggcaaat caaccaacca tgcgctgctg
     6481 gctctcttca gcaacatctg ttgaaaagaa ccctttaaaa atacaaaatt gtaagcaaat
     6541 cacaacttac cttttttcat acgtggaagc cgagaagttg aaaagaaccc tttaaaaata
     6601 caaaattgta agcaaatcac aacgaattga tggcactcct attaactact aaagttgaaa
     6661 agaacccttt aaaaatacaa aattgtaagc aaatcacaac ccccgttaac g
//
LOCUS       DAOSHM010000092         6632 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_10086_length_6632_cov_21.050783, whole genome shotgun
            sequence.
ACCESSION   DAOSHM010000092 DAOSHM010000000
VERSION     DAOSHM010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 6632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6632
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10086_length_6632_cov_21.050783"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(179..1567)
                     /locus_tag="PK839_09890"
     CDS             complement(179..1567)
                     /locus_tag="PK839_09890"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOG73084.1"
                     /translation="MAYRFARLLFAGLFFALPLSGYPQLTAPGRSWAGVTQYVNSPLH
                     QDSIFVFFSNVGSLRASHSTGNNASFKWYRYDPLAPSISERFVLFSTPSDGFFSQLDN
                     LAEGGYRVEVTDDADSTETFTAWLFTDNVTLTGIDVFSQCSFLQLTAISSPDQYAVEY
                     DRFVYYDLSRTSHTVNNKYGKDYFKVISWNASEPRVEVPDIQQLKLTLENPAPLWNSA
                     YTVTITNCFGRQLPVASTDVVTAIAAKSDMKIQVDANGVWTDWSSNERYEALLGLRLE
                     SLSVNVDSLYWQVVNEKIGKFANEYFVIWRDSSTVEQRKEAFPDKDLMAPGYYLVKHF
                     VINTTSGCRDSMVVNIEVDSSQIKADALPNVFSPNDDGVNDVFSFINPEENIKSIRTF
                     TIRILSRTGKQIYSYSGDPREWKGWDGRIEGSGAKAATGVYYFIIEARGWDDRTFKRG
                     PYKGFLHLYADR"
     gene            1989..5432
                     /gene="ileS"
                     /locus_tag="PK839_09895"
     CDS             1989..5432
                     /gene="ileS"
                     /locus_tag="PK839_09895"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HOG73085.1"
                     /translation="MSCSKFKEYKGLDLSQVNSEVLQAWDSNDTFHKSLETRSGHPRF
                     IFYEGPPSANGIPGIHHVMARTIKDVFCRYKTQKGFFVERKAGWDTHGLPVELGVEKM
                     LGITKEDIGTKISVEEYNQTCRREVMKYTDLWEDLTHKIGFWVDMKNPYITYDNRYIE
                     TLWYLLQQLFQKGLLYKGKTIQPYSPAAGTGLSTHELNQPGCYRDVKDTTVVAQFKLV
                     QNEQSSFLFNQTDSEIYILAWTTTPWTLPSNTALAVNPGIEYILVKTFNPYSGSPMTA
                     ILAKDRMDYYFSPANSNLSFDSFNAGDKNIPFRVIGSFKGSQLVGLRYEQLFPWVKPD
                     GDAFRIIAGDFVTTDEGTGVVHIAPTFGADDDRAAKASGIAPLILRDRFNSVEPMVDR
                     KGKFYKLEELSPDFVKSNVNIELYSEFAGRYVKNDYDPTLTDDDTTLDVDLAVSLKRD
                     GKAFRIEKHIHSYPHCWRTDKPVLYYPLDSWFIKVTALKDRMIELNNTINWKPASTGS
                     GRFGKWLENLLDWNLSRSRFWGTPLPIWVTEDRSEMICIGSVAQLKAEIERSVKVGFM
                     KSNPLDNFVENDNSAENYEKFDLHRPFVDTIVLVSASGKKMFREPDLIDVWFDSGAMP
                     YAQLHYPFENGERFNELFPADFVAEGVDQTRGWFYTLHALAALLFDSVAFKNIISNGL
                     VLDKNGNKMSKRLGNAVDPWQVIEEYGSDPLRWYMLTNSQPWDNLKFDVAGVDEVRRK
                     FFGTLYNTYSFFALYANVDGFRYAEEEVPVQNRPEIDRWILSLLNSLVREVENAYEAY
                     EPTRAGRAIQDFVTEQLSNWYVRLNRKRFWGGDYSTDKVAAYQTLYTCLETVAMLASP
                     MAPFYMDQLFTDLNAVTGRHTDQSVHLTDFPTYSNELIDKTLEEKMEIAQTVCSLVLG
                     LRRKVNIKVRQPLSKIMIPVLDERFRVQLDSVKDLILSEVNVKELEYLSDSSGILVKK
                     VKPNFKSLGPRFGKSMKSIATALSELSQADINTFERSGKLTLNIPEGSIDLLLSDVEV
                     LSEDIPGWLVASEGRITVALDITITEALRLEGIARELINRVQNIRKDSGFEVTDKIRI
                     TLKKHDKLNPAVEAHYDYIAGQTLAREIILTESELNGNAIVLDLDDDLITSICVEKIN
                     G"
     gene            5483..5860
                     /locus_tag="PK839_09900"
     CDS             5483..5860
                     /locus_tag="PK839_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HOG73086.1"
                     /translation="MGEKTRYSDEELAEFKELILQKLEKARNDYEILKSAITMSESND
                     TEDTSPTFKVLEEGASTLSKEEAGKLAQRQLKFIEHLEAALVRIENKTYGICRETGKL
                     ISKERLRAVPHATLSIDAKNNQR"
     gene            5946..6611
                     /locus_tag="PK839_09905"
     CDS             5946..6611
                     /locus_tag="PK839_09905"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005641523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HOG73087.1"
                     /translation="MRLTKQTTSALVVITVLLIDQLLKIWIKTHFAIGDEMRVFGNWF
                     ILHFTENPGMAFGMNFWGVWGKLILSIFRLAAIVLIGWYLNHVIKQGAKTGLVVGISL
                     VMAGAIGNMIDSAFYGLLFDSGTVYNHDMNGWVSYYGVSKLNFDGYAGFLRGCVVDML
                     YFPVINTVIPSWVPIWGGHQFQFFRPVFNIADSAITTGVIYLILFQRNNIIIKDKGKG
                     VKG"
BASE COUNT         1837 a         1523 c         1584 g         1688 t
ORIGIN      
        1 ttttccaaag agctgtacgg attctttgca tcgcaacggg aagaaataag tataaagcgt
       61 aaagggtaaa aaaaattcca aaacttgttt gcctgttgaa gaattagccc ttaaccaggc
      121 tttggaatcg aaaaacatct ccaattttaa aagggtaaca gggaaaaacc atgaaaattt
      181 acctgtcggc gtagaggtgt agaaaaccct tgtaggggcc acgcttaaag gtgcggtcgt
      241 cccagcccct agcctcaata atgaagtagt aaacccccgt ggcagccttg gctccgctcc
      301 cctcaatgcg tccgtcccag cccttccact cgcgcgggtc gccactgtag gagtatatct
      361 gcttgccggt tcggctaagt attctaatgg taaacgtccg gattgacttg atgttctcct
      421 cggggttaat gaaggaaaac acgtcgttta ccccgtcatc attgggtgaa aatacattgg
      481 gcagggcatc ggcttttatc tgcgaggagt caacctcgat gttcaccacc atggagtcgc
      541 ggcatccgga agtggtgttg atcacaaaat gctttaccag gtagtagcct ggcgccatca
      601 agtccttatc gggaaaagct tcctttctct gctccaccgt ggagctatcg cgccaaataa
      661 cgaaatactc gttggcaaac ttgccaattt tttcgttaac cacctgccag taaagggagt
      721 cgacgtttac cgaaagactc tccaggcgga gtcccaatag agcctcatac ctttcgttgc
      781 tgctccaatc ggtccacacg ccattggcat ccacctgaat tttcatgtcg gatttggcgg
      841 caatggccgt caccacatca gtagaagcaa ccggtagctg ccttccgaag cagtttgtaa
      901 tggttaccgt gtaagctgaa ttccaaaggg gggcaggatt ctcgagggta agcttaagct
      961 gctggatatc gggaacctcc acccttggtt cggaggcatt ccacgaaatc accttaaagt
     1021 agtcctttcc atacttattg tttaccgtat gagaggtacg cgaaaggtcg tagtacacaa
     1081 acctgtcgta ctcaacggca tactggtcgg ggctcgatat ggcagttagc tgcaggaagc
     1141 tgcactggct aaaaacgtca atgccggtta gtgttacatt atcagtaaaa agccatgctg
     1201 taaaagtctc agttgaatcg gcatcatcgg taacctcaac cctataacct ccctcggcta
     1261 agttgtcgag ctgagaaaaa aaaccatcgg agggagtgct gaagagcaca aatcgttcgc
     1321 ttatggaagg cgccagaggg tcataccggt accatttaaa tgaagcatta ttgcctgtag
     1381 aatgggacgc cctaagcgag ccaacatttg aaaaaaatac aaaaattgag tcctgatgca
     1441 atggtgagtt cacatactgc gtaacgcctg cccagctacg acctggagcg gtaagctgtg
     1501 ggtaaccgga caggggtaat gcaaaaaaca accccgcaaa aagtaacctt gcaaatcggt
     1561 aagccattgt gcaaacgtaa aattagctac aatattaacg aataaaaagt aaattagtaa
     1621 tcccagtgcc tgaatcgtta aatatgagcc ctatagtcga atcgtgaaaa ggtagagtag
     1681 aaggttaaca gctttatact tgcccggcac gtgtcagggg aacctgtctt aaacccgaca
     1741 agcatccgaa gccttcagta tcttttaagc cctatccttt atcacaaatt gaagcattat
     1801 gcgtattttt caaagctaca ccacaaattc ccaacccgtt actacaacgt ataagctttc
     1861 gattaatcat aatttcaata actttgtagg atttttaaaa gggctttttt gtttataact
     1921 aagtgttatt cagcaatata agttgatttc ataagtattt ttttgcacaa taagtaatta
     1981 gcaattagat gagttgttct aaatttaagg agtacaaggg tttagacctt tcacaagtga
     2041 atagtgaggt gctgcaggca tgggactcta acgatacctt tcacaagagt ttggaaacca
     2101 gaagtgggca tcctcgtttc atcttttacg aagggcctcc atcggcaaac ggaattcccg
     2161 gaattcacca cgttatggcc cgaaccatta aggatgtatt ttgccgctat aaaactcaaa
     2221 agggtttttt tgttgagcgt aaggcaggct gggacaccca tgggcttccc gttgagctgg
     2281 gggttgaaaa gatgctgggt attaccaagg aggatattgg aacaaagata tcggtggagg
     2341 agtataacca aacctgccgc agggaggtaa tgaagtacac cgacttgtgg gaggacctta
     2401 cccataaaat cggtttctgg gttgatatga aaaaccccta catcacctac gataaccgat
     2461 atatagaaac cctttggtac ctactccagc agctatttca gaaagggttg ctctacaagg
     2521 ggaaaaccat tcaaccctac tcgccggctg ccgggacagg gcttagcact catgagctga
     2581 accagcccgg atgctaccgc gacgtgaagg ataccactgt tgtagcccag tttaagctcg
     2641 tccaaaatga gcaaagcagc ttccttttca accaaaccga ttcggaaatt tacattctgg
     2701 cgtggactac tacaccctgg acactcccat caaacactgc tctggcggtt aaccccggta
     2761 tagaatacat actggttaaa acatttaacc cctatagcgg aagcccaatg acggccattc
     2821 tggctaaaga ccggatggac tactacttct caccagccaa ctcaaacttg tcgttcgata
     2881 gctttaacgc aggcgataag aatatacctt tccgggtgat aggcagcttc aagggcagcc
     2941 agctggtagg gttacgctat gaacagcttt ttccatgggt taaacccgat ggcgatgcct
     3001 tcaggataat agcaggtgat tttgttacca ccgacgaggg aacaggcgtt gttcatattg
     3061 cccccacttt tggtgccgac gacgaccgtg cagcaaaagc ttcgggaatt gccccattaa
     3121 ttcttcggga taggttcaat agcgttgagc caatggttga ccgcaaaggc aaattttaca
     3181 agctggagga gcttagcccc gattttgtca aatcaaatgt aaacatcgag ctctatagcg
     3241 aatttgccgg tcgttacgtt aaaaacgatt acgaccctac ccttaccgat gatgatacta
     3301 cgcttgatgt tgaccttgcc gtttcgctga agcgcgatgg aaaggctttc cgcatcgaaa
     3361 agcatattca tagctacccc cattgctggc gcaccgacaa accggtgctt tactacccac
     3421 ttgacagctg gtttattaag gtaactgcgt taaaggatag gatgatagag ctgaataaca
     3481 ctatcaactg gaagcctgcc agcacgggaa gcggacggtt tggtaaatgg ctcgagaacc
     3541 tgcttgattg gaacctttcg cgctcccgtt tctggggaac tccactccca atttgggtta
     3601 ccgaggatcg ttcggagatg atctgcattg gttccgtggc gcagctcaaa gccgagattg
     3661 aacgaagcgt taaggttgga ttcatgaaaa gtaacccgtt ggataatttc gttgaaaacg
     3721 acaatagcgc ggaaaactat gaaaagttcg atcttcacag gccatttgtc gataccattg
     3781 tgctggtttc ggctagcggc aaaaagatgt ttcgcgaacc cgatctcata gacgtttggt
     3841 tcgactccgg ggctatgccc tacgcccagc ttcactaccc gttcgaaaat ggcgaaaggt
     3901 tcaacgagct cttccctgcc gattttgttg ccgaaggggt tgatcaaacc cgtgggtggt
     3961 tctacaccct tcatgccctt gccgccctgc tgttcgattc ggttgcattc aaaaatatca
     4021 tctccaacgg gttggttctc gataagaatg gtaacaagat gagcaagcgc cttggaaatg
     4081 ccgttgaccc gtggcaggtt attgaggagt acggctccga cccgctgcgc tggtacatgc
     4141 tcaccaactc ccaaccctgg gacaacctga aatttgacgt ggctggtgtt gacgaggtaa
     4201 ggcgcaaatt ctttggcacc ctctacaaca cctactcatt ttttgccctt tacgctaatg
     4261 ttgacggatt tcgctacgcc gaggaggaag tccccgttca aaatcgtccc gaaattgacc
     4321 gctggatttt gtcgctccta aactcgctgg ttagggaggt tgagaatgct tacgaagctt
     4381 acgaacccac ccgtgcaggg cgcgcaatac aggattttgt taccgagcag ctgagcaact
     4441 ggtacgtaag gcttaaccga aagcgctttt ggggaggcga ttacagtacc gataaggtag
     4501 cagcttacca aacactctat acctgcctcg aaaccgtggc aatgcttgca tcaccaatgg
     4561 ctccatttta tatggaccag ctctttaccg atttaaacgc cgtaaccggg agacatacgg
     4621 atcaaagcgt tcacctgacc gacttcccca cgtactccaa tgagttgata gacaagaccc
     4681 tggaggaaaa aatggaaatt gcccagacag tctgctcgct ggttctgggc ctgcgtcgta
     4741 aggttaacat taaagtccgg cagccactga gcaaaatcat gatccctgtt cttgatgaaa
     4801 ggttccgcgt gcagcttgat tccgttaaag acctcattct tagcgaggtt aacgtaaagg
     4861 agcttgaata cctgtccgat tcgtcaggca tactggtgaa aaaggttaag cccaatttta
     4921 aatcgctagg cccgcgtttt ggtaaatcaa tgaaaagcat tgccactgcc ctgtcggagt
     4981 tgtcacaagc cgatatcaac acctttgagc gtagcggcaa gttaacctta aacattccag
     5041 aaggaagcat tgatctgctg ctcagcgatg tagaagtgct gtcggaggat atccctggct
     5101 ggctggtagc cagcgaaggc cgtataaccg tagctctcga tattaccata acagaggctt
     5161 tacggcttga gggcatagcc cgtgaactca tcaacagggt tcagaacata cgtaaggatt
     5221 cggggtttga ggtaaccgac aaaattcgca tcaccctgaa gaaacacgat aagttgaacc
     5281 ctgccgtgga agcccattac gattatattg ccggacaaac cctggccagg gagataattt
     5341 taaccgaaag tgaactaaat ggcaacgcca tcgttttaga ccttgacgat gacttgataa
     5401 cctcgatttg tgtggaaaaa atcaacgggt agcgatattt tttgaaacga tattttttat
     5461 aattttagca aattttacag ccatgggaga gaaaacaaga tattcggatg aggagctggc
     5521 ggaatttaag gagttgattc tgcaaaagct cgaaaaggcg cgcaacgact atgaaattct
     5581 aaaaagcgcg ataaccatga gcgaaagcaa cgatactgag gatacttcgc ccacttttaa
     5641 agtgctcgag gagggagctt caaccctttc gaaagaggaa gccggaaagt tggcccagcg
     5701 ccagctgaag tttattgaac atcttgaggc tgcgttggtt cgcatagaga ataaaaccta
     5761 cggtatctgc agggagacag gcaagctgat ctccaaggag cggctacgag ctgtgcccca
     5821 tgccacccta agcattgacg ccaaaaacaa ccagcggtag ctgattaatc gatacatcca
     5881 tgaaatgcgg ccttttggcc gcttattttt ttttagtagc tttgcgcaaa tttttacact
     5941 aacctatgcg gcttacaaaa cagaccacct cggcactggt agttattaca gttcttctta
     6001 ttgatcagct acttaaaatt tggattaaaa cccattttgc cattggcgac gagatgaggg
     6061 tatttggaaa ttggttcatc ctgcatttta ccgaaaatcc gggcatggca ttcggaatga
     6121 atttttgggg agtatggggc aagctgatct taagtatttt caggttggca gctattgttc
     6181 ttattggttg gtacctgaac catgtcatca aacagggagc caaaacaggc ttggtggttg
     6241 gcatatcgtt ggtaatggct ggtgccatag ggaacatgat cgacagcgct ttctacggcc
     6301 tactgttcga ttcgggaacc gtttacaacc acgatatgaa cggatgggtt tcctactacg
     6361 gcgtatccaa gctaaatttt gatggctatg caggtttttt aagggggtgt gtggttgata
     6421 tgctttactt tccagtgatt aatacggtta ttcccagctg ggtgccaatt tggggagggc
     6481 accaatttca attcttccgt cctgtattca atattgccga ttcggccatt accacagggg
     6541 ttatttacct gatcctgttc caaaggaaca acattattat aaaggacaaa ggaaaagggg
     6601 taaaggggta aaggttaata caaggttaaa gg
//
LOCUS       DAOSHM010000093         6614 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_10132_length_6614_cov_8.363470, whole genome shotgun sequence.
ACCESSION   DAOSHM010000093 DAOSHM010000000
VERSION     DAOSHM010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 6614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6614
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10132_length_6614_cov_8.363470"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..839)
                     /locus_tag="PK839_09910"
     CDS             complement(<1..839)
                     /locus_tag="PK839_09910"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOG73088.1"
                     /translation="MFKIKPFHLFSLLLSAVVSLIYSCTNDNNTSYLELKQVKIGEQV
                     IDIVNPQLNTGIETDTAIELTFSARVDSVSLRKNLNLKLLDSTIAYRLSYSDAGKTTI
                     KVIPIENILYNHDYYILISNSLRGMNGELFMGITIKFSTRSASIAVKSIDIEGNSYAL
                     NQIIRNVDFNPSFKIYFTKQLDLSSIGSSEFKINDVEGRGYTITVNTINDSLIEIQPH
                     QPLEHVLRYSLFIPSTIRGKFNEQFSGSVFQFYTMIDSTPKYPVIPDDDLLTLIQQQT
                     FRY"
     gene            complement(903..2141)
                     /locus_tag="PK839_09915"
     CDS             complement(903..2141)
                     /locus_tag="PK839_09915"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOG73089.1"
                     /translation="MRKCAKSILMIMSVVALGFFTACNNDDEEVAFELKSLMAGSVDL
                     NGATGAVNVPKDAVITATFSKDVDPQTLNITLKQTYDNAEFDVTVNVNAKVVTITPAA
                     LGEGTGYTIYFNTVKSTKGDVLPTFNRSFTVAGSFVPTGQIAYWNFENNANDQVGTWN
                     PSANGVVDVTYNDSRKTAAGKAATFNGTTSIIEIPNADALINTHDFTISFWVKTNSTG
                     ITHGHFVMGLGAFYGIQFEIFGGYDGAKFAISYNIADTATAAEDMWFPANAQDNTNGG
                     WQGCTYAKVITPENMQLLLKDAWLQVVYTYNSTEKEGILYYNGEKMKSFDFDLWPDGD
                     KKRGVVGLKYGGKEPDVVNELAFGFIQSRAGSMWDAEPWGGYDFPDANHFKGQLDDVR
                     FFHKALTAQEVDLMYKSEKP"
     gene            complement(2206..3699)
                     /locus_tag="PK839_09920"
     CDS             complement(2206..3699)
                     /locus_tag="PK839_09920"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOG73090.1"
                     /translation="MKTLAYLTKIAAAISLTILFASCEDFLDKPPQGALTQSNFPKSA
                     NDALLATNAVYNTLRNSNYNEGLFPILDIMSDDARKGSNPDDQAASIGLFEIFGHTET
                     EWVIGSWWNTLYLGVKRANVVTEKLPEIEMDETLKQRYIGEAKFLRALFYFDLVRAWG
                     DVPLYTTTQPDMSMGRSPAADVYNQILADLADAISVLPEKSEYSGNDIGRATKGAAKA
                     LLAKVYLFLHDYENAEKYALEVINSHQYDLEPLFSDAFSILNEHGIESVFEVGAVGQE
                     GIENGGNQYANVQGVRGTPNRGWGFNRPSLDLINSFEANDPRKDATVIFLGEVLDGVT
                     ILGDGKTPDETYDSQGNLIEIECYNQKVWTPGQTVPPSFGHNRRIIRFADVLLIAAEA
                     LNENNKSDIALPYLNRVRQRARQGNDAILPDITITDKDQLRDIILNERRHELALESQR
                     FWDLVRTGKATQVLGPLGYITGKHELLPIPQAELDLTGNKWPQNPLW"
     gene            complement(3712..>6614)
                     /locus_tag="PK839_09925"
     CDS             complement(3712..>6614)
                     /locus_tag="PK839_09925"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOG73091.1"
                     /translation="VSGRVTDAETGETLPGVNILILGKTKGTITNNDGSYTINMEEDE
                     NTLVFSFIGYRPDTITVTTSRRVDVQLKVEALFIEDIVVVGYGVQRKSDLTGSVVSVK
                     SEELTRIPTSSPMQALEGKVPGLQVASSSGAPGSGVVVRVRGVGTFNNSNPIYVVDGV
                     ILDNIDYLNTADIESMEILKDASATAIYGSRGANGVIIVTTKKSKLGTDKTTFNLSYE
                     NSYQKLINRIDLLSGREFATVVNVINPGTYNNIDAVPNTNWQDIIFDDGFSAPIHNIQ
                     FSASGATSKMQYYVGIGYYNQTGIINKSNFERLTVKLNNTYQLNKWLNIGNNVTIVPF
                     KQQNTNGNVVFVAYRAWPVLEPYQADGSYTALPGTGNPLADIEYTNSFSNGLKGTGNI
                     FGDATLTKGLVLRSSFGVDLGFNRSKSFSPTYFVSPQQQNSENDLYKGEGTQSSWIWE
                     NTLNYNHDFDRHHFDALAGFTMQDVTSENIGISGINVIRDAEDFWYINSNNINPNSVY
                     NGIDASYYFSMISYLFRVNYSYNSRYLLTATYRVDGSSKFTKDNRYSGFPSVALGWNV
                     HNESFLKNSMTINNLKLKISWGKIGNEKISYDKQYSLVGSGLDAVFGIGDFIIPGQTY
                     ETTGNPNLVWETTSQSNIGIELGLWDNKLTAELDYFSKTTSDILIALEIPGYIGNGSG
                     STITRNAAEVLNSGFEFNIRWNDHIGEFRYSVGLMGNTLHNEVKKISGSGATDDYLLG
                     GDGFTRSVVGKPVGAFYGYKVDGIFQNQAELDAYPHRSNAGIGDLRFVNTNGDNVLTD
                     ADRTYLGSPIPTLLMGLNLNASYKNFNLSIDVQSQFGSKIYNVKETVRPDLYNFEKHV
                     KDFWRGEGTSNSEPRPTSGGYNFLPSERFVQDGSFIRLRNVSVGYSIPKKFTDSINAG
                     NIYVYLSGTNLLTFTKYTGYTPEILGGPIDNALDYGTYPVSSIFSAGIRVTF"
BASE COUNT         1882 a         1433 c         1355 g         1944 t
ORIGIN      
        1 aagtagcgga aagtttgttg ctggataagc gtaagcaggt catcgtcggg aataaccggg
       61 tattttggag tgctgtcgat cattgtgtaa aactgaaaaa cactcccgct aaactgttca
      121 ttaaatttcc ccctaatagt tgaagggatg aaaagagagt atcttagaac atgctctagc
      181 ggttggtgtg gttgtatttc aataagactg tcgttgatcg tattaaccgt aatggtatag
      241 cccctcccct caacatcatt aattttgaat tcagaggagc caatactaga taaatcgagt
      301 tgttttgtaa agtatatttt aaaacttgga ttaaaatcga cgtttctaat aatctggttt
      361 aaagcatagc tgttaccttc aatgtcaata gattttacag ctatcgaagc gcttcttgtt
      421 gaaaatttta tggtaatacc cataaataat tcaccgttca tgccccttaa actgttcgat
      481 atcagtatat agtagtcatg attatacagt atgttttcta ttggaatgac tttaatagtt
      541 gttttacctg cgtcggaata agaaagtcta taagcaattg ttgaatctaa aagctttaga
      601 tttaaatttt ttctgagaga aacagaatct actctagcgc taaatgtcaa ctcaatagct
      661 gtatcggttt cgatgccagt attaagttga ggatttacta tatcaataac ctgctcacca
      721 atttttacct gcttgagttc cagatagctg gtattattat catttgtaca tgaatatatt
      781 aaagacacta ctgctgatag caatagacta aataggtgaa aaggtttaat tttaaacata
      841 tactctagga attaaaaaaa tcggagcaag catcccaaat agcttgctcc gattttgaaa
      901 gtttaaggtt tttctgattt atacatcagg tcaacctcct gggctgttaa tgccttgtgg
      961 aagaatctaa catcgtccag ctgaccctta aagtggttgg catcagggaa atcataacca
     1021 ccccaaggct cagcatccca catactgcca gcgcgggatt gtatgaaacc aaaagccaac
     1081 tcgtttacaa catcgggttc cttaccaccg tacttcagac ccacaacgcc tctcttttta
     1141 tccccatcgg gccacaggtc gaaatcgaaa cttttcatct tttcgccatt atagtaaagg
     1201 atgccctcct tttctgtgct attataggtg taaacaacct gaagccaggc gtctttcagc
     1261 aaaagttgca tattctcagg agtgataacc tttgcatacg tgcatccctg ccagccacca
     1321 ttggtattat cctgggcgtt ggcggggaac cacatatcct cagctgctgt agctgtatcg
     1381 gcaatattgt agctaatggc aaatttggcg ccatcgtaac cgccaaaaat ttcaaactgg
     1441 atgccgtaga aagcgccaag gcccattaca aagtggccat gtgtaattcc tgttgagttg
     1501 gttttaaccc agaagctaat ggtaaagtca tgggtgttaa tcagcgcgtc agcgttggga
     1561 atttcaatga tactggtggt accattaaag gtagcggctt tccctgctgc agttttcctg
     1621 gaatcgttgt aagtaacgtc aactacacca tttgcagaag gattccatgt tccaacctgg
     1681 tcattggcgt tgttttcaaa gttccagtag gcaatttggc ctgtgggaac aaatgaacct
     1741 gcaacagtaa aggatcggtt gaaagtgggg agcacatcac ccttggttga cttaaccgtg
     1801 ttgaagtaaa tggtgtaacc tgttccttcg cctaaagcag cgggtgtaat ggttacaacc
     1861 ttagcgttaa catttacagt aacatcaaac tcagcattgt cgtatgtttg cttgagagtg
     1921 atgttaaggg tttggggatc aacatccttt gaaaaggttg ctgtaataac agcatccttg
     1981 ggaacattta ctgctccagt tgcaccatta aggtcgacag agcctgccat cagcgacttc
     2041 agttcaaaag ccacctcttc gtcgtcattg ttgcacgcgg taaaaaatcc aagggcaaca
     2101 accgacataa tcatcaagat tgattttgca cactttctca tagctaaatt aatttaggtt
     2161 aataaatgaa tgtaaactga cactatatat gtttaaattt agaagttacc atagtggatt
     2221 ttgaggccat ttgttaccgg ttaggtcgag ttcagcctga ggaatgggga gcaactcatg
     2281 tttaccagta atataaccaa gaggaccaag cacctgtgtg gctttccctg ttcgcaccaa
     2341 atcccaaaac cgctggcttt ctagcgctaa ctcatgcctt cgctcgttga gaattatgtc
     2401 gcgaagttga tccttatcgg taatggttat atcgggaagg atggcatcgt tgccctgtct
     2461 tgccctttgc ctaaccctgt taaggtaggg aagagcgata tcggatttgt tattctcatt
     2521 cagagcctcg gcggcaatta acagtacatc ggcaaagcga ataattcttc tgttatgacc
     2581 aaacgatggt ggtacagtct gaccaggtgt ccaaaccttc tggttgtaac attcaatctc
     2641 aatcagattt ccctgactat cgtaggtttc atcaggtgtt tttccatcgc ccagaattgt
     2701 aacaccgtcc agaacctcgc ctagaaaaat aaccgttgca tcctttctgg gatcatttgc
     2761 ttcaaaggag ttgatgaggt caagggatgg gcggttaaac ccccagcccc tgtttggagt
     2821 tcccctaacg ccctgaacat tggcgtactg gttgccgcca ttctcaattc cctcctgacc
     2881 aacggcgcca acctcaaaaa ctgactctat tccatgttca ttaaggatgc taaaggcatc
     2941 gctaaatagt ggttcaaggt catattgatg cgagttgatt acttccaggg cgtatttttc
     3001 agcattttca tagtcatgaa ggaatagata aacctttgcc agcaaggcct tagctgctcc
     3061 tttggttgcc ctgccgatat catttccact atactcgctt ttttcaggaa gaacactaat
     3121 ggcatcggca agatcagcta aaatctgatt ataaacatcc gctgcaggtg atcttcccat
     3181 gctcatgtcg ggttgagtgg tggtgtagag cggaacatct ccccatgccc taacgaggtc
     3241 aaagtagaag agcgcccgta gaaactttgc ctctccaata tacctctgtt tcaaggtttc
     3301 atccatttca atttcgggta gtttttcagt aacaacattt gcccgcttca caccaaggta
     3361 gagcgtattc caccaactac caataaccca ttcggtttca gtatgcccaa aaatttcaaa
     3421 aaggcctata gaggcagctt ggtcatcggg attacttccc ttacgggcat catcggacat
     3481 gatatcaagg attggaaata aaccttcgtt gtagttagaa ttacggaggg tgttgtaaac
     3541 agcattagta gcaagcaatg catcgttagc agattttgga aagttacttt gggttaaagc
     3601 cccctggggt ggtttatcca aaaagtcttc gcatgaagca aatagaatgg ttaaactgat
     3661 agcagctgca atttttgtta ggtatgctag tgttttcatg gcttgcacaa tttaaaaggt
     3721 tactctaata ccggcagaaa agattgacga aacaggatat gtcccatagt ccaaagcgtt
     3781 atcaataggt cccccaagta tctccggtgt atatccggta tattttgtga aagtaagcag
     3841 gttagtgcca cttagatata cgtaaatatt acccgcgttt atcgaatcag taaacttctt
     3901 gggtatggaa tatcccactg aaacatttct aagccttata aatgaaccat cctgtacaaa
     3961 gcgttcggac ggcaggaagt tatatccacc cgaagtagga cgaggttcag agttgctggt
     4021 cccctcacct ctccaaaagt ccttaacgtg cttttcaaag ttgtaaaggt cgggacgtac
     4081 ggtttcttta acgttgtata ttttactacc aaactgagac tgaacatcaa tgctcaggtt
     4141 gaaattttta taggaggcat ttaagtttag ccccattaac agggttggaa tgggagatcc
     4201 caggtaggtt cgatcggcat cggtcagaac attatcgcca ttggtgttta caaatcgtaa
     4261 atcgcctatt ccggcgttac ttctgtgggg gtaggcatcc agttcagcct ggttctggaa
     4321 tatgccatca accttgtagc cgtaaaaggc cccaacaggc ttacccacaa cggatctggt
     4381 gaacccgtca ccaccaagca ggtaatcgtc agtagctccc gaaccactaa tctttttaac
     4441 ctcattatgc agggtatttc ccatcaaacc aacagagtac ctgaattccc ctatgtggtc
     4501 gttccaacga atgttaaact cgaatccgga attgagaacc tcagcagcat tccttgttat
     4561 tgttgatcca cttccgtttc ctatatagcc ggggatctcc agggctatca gaatatcact
     4621 ggtggtttta ctaaagtaat ccaactcagc agttagcttg ttgtcccaaa gtcctaattc
     4681 tattcctatg ttggattggc tggttgtctc ccaaacgaga tttggatttc cagttgtctc
     4741 ataggtttgg ccgggaataa taaaatcacc aattccaaac acggcatcta aacctgagcc
     4801 aacaagagaa tactgcttat cataggaaat cttctcattt ccaatctttc cccagctaat
     4861 tttaagcttt aaattattga ttgtcatact attcttcaag aatgattcgt tgtgtacgtt
     4921 ccagcccaat gctaccgaag ggaatccaga ataccggtta tccttggtaa actttgagga
     4981 accgtcaaca cggtaggtgg cagttagcag gtatcggcta ttgtaggagt agttaaccct
     5041 aaaaaggtat gaaatcatag aaaagtagta ggaggcatca atgccattgt aaactgaatt
     5101 tgggttgata ttgttggagt tgatgtacca aaaatcttct gcatccctaa taacattaat
     5161 accgctaatt ccaatatttt cggaagtaac atcctgcatt gtaaaaccag ccaacgcatc
     5221 gaagtgatgc ctatcgaaat catggttgta attcaaggtg ttctcccaaa tccaagagct
     5281 ctgagtccct tcacccttgt atagatcgtt ttctgaattc tgttgttgag gagatacaaa
     5341 ataagtggga gaaaaggatt tgcttcggtt aaagcccaaa tcaacaccaa agctagagcg
     5401 aagtactagt cctttagtta aagtagcatc tccaaaaata ttgcctgttc cctttaaccc
     5461 attgctgaag ctgttggtgt attcaatgtc ggccagtgga tttcctgtgc cgggaagtgc
     5521 agtataggaa ccatctgcct ggtatggttc taaaacaggc catgcccggt aagcaacaaa
     5581 taccacatta ccattggtgt tctgctgttt gaagggaaca atcgttacat tattcccaat
     5641 atttagccac ttgtttagct ggtatgtatt gttaagttta acagttagtc tttcaaaatt
     5701 cgacttattg attatcccag tctgattgta atagccaatg cccacatagt attgcatctt
     5761 ggatgttgcg ccagaagccg aaaactggat gttatgaatc ggagcgctaa acccgtcgtc
     5821 aaaaataata tcctgccagt tggtgttcgg aacagcatca atgttgttgt acgtgccagg
     5881 gtttattaca ttaacaacag tagcaaattc cctaccagat aacaaatcaa ttctatttat
     5941 caacttctgg tagctattct cataggagag gttaaaagta gttttatcag tgccaagctt
     6001 agatttcttt gtggtaacaa tgataacacc attggctccc ctggaaccgt aaatagccgt
     6061 tgccgaagca tccttaagga tttccattga ttctatatca gcagtattca ggtagtcgat
     6121 gttgtctaga ataacgccat ccaccacgta gattgggtta gagttattaa acgtacctac
     6181 gccacgcacc ctgacaacca cccctgaacc aggggcacct gaggaactgg caacttgaag
     6241 cccagggact tttccttcca aagcctgcat gggtgacgag gtaggaatac gtgtcaactc
     6301 ttctgattta acacttacaa cagaacccgt taaatcgctc ttacgctgaa caccataacc
     6361 tacaactaca atatcctcaa taaacaaagc ctctacctta agctgaacat caacccttct
     6421 actagttgtt acggtaatag tatcaggtct atagccaata aaggaaaata ccagagtatt
     6481 ttcgtcctct tccatgttaa tggtataaga accatcatta ttagtaatag tacccttggt
     6541 tttacccaaa attaaaatgt ttactccggg cagcgtttcg ccagtttcgg catcggtaac
     6601 ccttccgctc actg
//
LOCUS       DAOSHM010000094         6511 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_10355_length_6511_cov_19.918680, whole genome shotgun
            sequence.
ACCESSION   DAOSHM010000094 DAOSHM010000000
VERSION     DAOSHM010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 6511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6511
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10355_length_6511_cov_19.918680"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(65..1831)
                     /locus_tag="PK839_09930"
     CDS             complement(65..1831)
                     /locus_tag="PK839_09930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOG73092.1"
                     /translation="MRIFGANISLQVCTFLKEFLKETSSFENANIMLFDAFGINTFFT
                     SNDDFQGLKESVSLVAIGSVEEPDRAEYGDFQTNKNLSNKVVNYLVKRNISPEIIIEP
                     TCGNGNFIVASLSNFKAAKKVFGIEIYKPYVWETKFSILDFYLSNPDNHKPEITITHC
                     SVFDFDFKNISKQFPAEKILIIGNPPWVTNSKLGSLNSNNLPKKSNFKNQNGLDAITG
                     KGNFDIAEYITLMLLDTFQTHNGYLALLVKNSVIKNIVFDQKDKKYRIGEIEKYCIDS
                     KMEFNVSVEAALLYCQLNLTPSFECNEFDFYSFEKRLSFGWLNSKFVSNLADYNETKG
                     IDGLCPFEWRQGIKHDCAAIMELERINDHFVNNQSDEIKLDEDLVYGLLKSSDLKNSV
                     IMNSRKYTIVTQTKVGQETSYIQHLYPDTYSYLSQNISIFQARKSSIYNGNPLFSIFG
                     IGDYSFKPFKVAISGLYKTYHFSLVLPQNNKPMMLDDTCYFIGFDKIEYAVYSFILLN
                     SKTTEAFLKSITFPDAKRIFTKDILMRIDILSLSKVIPQNEIEKQIVFLNGKYNFDIN
                     LSQWDSFISEMTPKKEKQLKIF"
     gene            complement(1831..2505)
                     /locus_tag="PK839_09935"
     CDS             complement(1831..2505)
                     /locus_tag="PK839_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008622274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HOG73093.1"
                     /translation="MKEKLTIEKLKAEAKVFCLTESKIQNKSLFGVTDGKAVGTYIEH
                     KFQEQLNAKYKITIGSSANGIDLPSDDILTDIKVTSIKQPQSSCPFIDAKQKIFGLGY
                     NLLVFVYDKIDDFQSKTTRLNFVSCSFILKERTSDYTTTYRLREMVKDKANVEDIVAY
                     LNDKNIPADEITLNQLAKQILKTPPVQGYLTISNALQWRLQYQRVVTLEKAVQGISRI
                     IDKVVR"
     gene            complement(2621..2902)
                     /locus_tag="PK839_09940"
     CDS             complement(2621..2902)
                     /locus_tag="PK839_09940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG73094.1"
                     /translation="MSCKFNDGFVDCAGREVWMRLPNDGSPAAAREIDSADVHSFNRT
                     KVKPLEIVYIYPIGKFTDDIKCGQRPLHENDEVTDFAEQGACLLALNNR"
     gene            3595..3927
                     /locus_tag="PK839_09945"
     CDS             3595..3927
                     /locus_tag="PK839_09945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG73095.1"
                     /translation="MKKLLILLCFLGFVNLTLSAQNIYIRGHLQVIYIGPEGMDVICN
                     PPYDRDCVSIISEPGLPAKATAEVSDKGVTKIQPRVQVVGETLNSNGLPTKIYRIRKD
                     AVKEVVKN"
     gene            3977..5494
                     /locus_tag="PK839_09950"
     CDS             3977..5494
                     /locus_tag="PK839_09950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG73096.1"
                     /translation="MRNILTTFVFLCLSLPAISRVGGVEIFFAIKPSDCISCMAPIRD
                     ILAEIEKQELMEQVTFIVRDVPEKALPKYTKEVLKIPNSINVVSSEPLFSKYSFYNAS
                     AITVIDNSTVPIQRITFPLAYYFVNKTMFVERLSKSNKTIVALERVKLKESLGGIRML
                     SISNDSLLFINDRLFNTISLFSAGSGKLVGNFTLDSAWYAAPYSKDTFKLRQIWMNQH
                     VLERASYPQYYFKGVFNHRGRTIISAICYYPFTTGKDFFILPKNLFFMLDTLLMPISY
                     RWLYIDKETPIVNFYNAVFFQDDNLITTTFDVVNNRLNFNPDFLYYFKPEGDSLIFDK
                     SLHVEPPSFIKNGYSMNGFSLNYISEYMGNITVWFSYYPEFFDLRLEKWVSIDTGYVD
                     SKISTNTFEQGQPVNFQVLGVWAEANQYSMILRFGLQVYYMVQADKNIIQSALIYTLS
                     EGNQLQMVRKSKNEFVGTDYNSKTGDTEIIKFTINGVQTFISPSLNEACAFVVPM"
     gene            complement(5851..>6511)
                     /locus_tag="PK839_09955"
     CDS             complement(5851..>6511)
                     /locus_tag="PK839_09955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG73097.1"
                     /translation="RLNTDNHPYPVFLFTMSYLGQFGSNHLVWMLVGLAVVLLPVFFL
                     GKPLRGMFLAGFSGASAEMIIILMFQVLFGFLYAGIGLIVALFMAGLAVGAYVLPRFI
                     RLSVGSLTIAMAGYFALIPLIWMLRDVAAVWLLLLVISLFTLIPSVLVGYQYVLWTSA
                     VADRANPAAMSYSADLWGSTLGVVVVTLALIPLLGVVQTAAVLAALNLAGRLLIQPRN
                     R"
BASE COUNT         2002 a         1257 c         1216 g         2036 t
ORIGIN      
        1 ctctccgcca gctggcggat cggttcatcc ctgtttgtgc aagctgcctt acatgagtgt
       61 ttcatcagaa tatttttaac tgtttttctt tcttaggcgt catttcgcta ataaaactat
      121 cccattgcga caggttaata tcaaaattat acttgccgtt taggaaaact atttgctttt
      181 caatttcatt ttgtggaatt acctttgaaa gggataatat atctatacgc attaaaatat
      241 ctttggtaaa aattctttta gcatctggaa atgtaattga ttttaaaaac gcttctgttg
      301 ttttagaatt taaaagtata aacgaataaa ctgcatattc tattttgtcg aaaccaataa
      361 aataacaagt atcatctagc atcattggtt tgttattttg tggtaacact aatgaaaaat
      421 gatatgtttt atacaaaccc gaaattgcaa ctttgaatgg tttgaaagaa tagtcaccta
      481 taccaaatat tgaaaataat ggattgccat tataaatact agattttctt gcttgaaaaa
      541 tggaaatatt ctgcgataaa taagagtatg tatcaggata taaatgctga atatatgagg
      601 tttcttgccc aacttttgtt tgtgtaacaa tagtatattt tcttgaattc attattacag
      661 aattttttaa atcagaactt tttaaaagac cataaaccaa atcttcatcg agttttatct
      721 catcagattg gttattcaca aaatggtcgt ttattctttc taattccata atggcggcgc
      781 aatcgtgctt tatcccctgc ctccactcaa aagggcataa gccatcaatc cctttcgttt
      841 cattataatc ggcaagatta gatacgaact tagaattaag ccatccaaag cttaatcttt
      901 tttcaaaact atagaaatca aattcattac actcgaaact tggggttaaa ttaagctggc
      961 aataaagcaa agccgcttca actgaaacat taaattccat tttactatca atgcaatatt
     1021 tttctatttc acctattcta tactttttat ctttttggtc gaaaacaata ttttttatca
     1081 cggagttttt taccaatagc gccaaataac cgttgtgcgt ttgaaaggta tctaaaagca
     1141 ttaaagtaat atactcggca atatcaaaat tgcctttgcc tgtgattgca tctaacccgt
     1201 tttgattttt gaaatttgat ttttttggaa gattattcga attaaggcta cctaattttg
     1261 agtttgttac ccaaggcggg ttcccaatta ttaatatttt ttcggctgga aactgttttg
     1321 aaatattttt gaaatcaaaa tcgaaaacgc tacaatgtgt gattgtaatt tcaggtttgt
     1381 gattatcagg atttgagaga taaaaatcga gaatactgaa ttttgtttcc caaacgtaag
     1441 gtttataaat ttcgattcca aatacttttt tagctgcttt aaaattcgat aacgaggcaa
     1501 ctatgaaatt tccatttcca caagttggct caataataat ttcgggggat atatttcttt
     1561 ttactaaata gttcacaacc ttatttgata gatttttatt tgtttgaaaa tctccatact
     1621 ctgcacggtc tggctcttca actgaaccta tagcgacaag agaaacagat tctttgagtc
     1681 cttgaaaatc atcattactt gtaaaaaatg tattgatgcc gaaagcatca aacaacatta
     1741 tattggcatt ttcaaaagat gatgtttctt ttaaaaattc ttttaaaaac gtacaaactt
     1801 gcaatgaaat atttgcacca aatattctca ttacctgacc actttatcaa ttattctact
     1861 tataccttgc accgcttttt caagcgtaac aactctttga tattgaagtc gccattgtag
     1921 agcgtttgaa atagttaaat acccttgaac cggcggtgtt tttagaattt gtttagctag
     1981 ctgatttaga gttatttcat cggcaggaat atttttatcg tttaggtagg caacaatatc
     2041 ttctacatta gccttatcct taaccatttc acgcaaacga taagttgttg tataatcgga
     2101 agttcgttct tttaaaataa atgagcaact aacaaagtta agtctagtag ttttggattg
     2161 gaagtcatca attttgtcgt aaacaaatac aagtaggttg tatccaagtc caaaaatctt
     2221 ttgtttagca tctataaatg gacaagatga ttgaggttgt tttattgatg taaccttaat
     2281 gtccgttaat atgtcgtcag atggcaaatc aattccattg gctgaagaac ctatggttat
     2341 tttatatttt gcatttaatt gctcttgaaa cttatgttca atataagtcc caactgcttt
     2401 tccgtcagtt acgccaaaaa gtgatttatt ttgaattttc gattcagtta agcaaaaaac
     2461 tttggcttct gctttaagtt tttctattgt cagtttttct ttcatctgat ttccttacat
     2521 taagttacaa atttaatcaa tttttcagtc cgatttgtac gctgtcagtt ttctttgcat
     2581 gaggcataac aggcaggcag ctggttttgt catattaggt ttatctgttg ttcagtgcta
     2641 gtaggcaggc tccttgctcg gcgaagtctg taacttcgtc gttttcatgc agcggtctct
     2701 gaccgcattt gatatcatca gtaaactttc caatggggta aatatacacg atttcaagcg
     2761 gtttaacttt cgttcggtta aagctgtgta catcagccga atctatttcc ctcgctgccg
     2821 ctggacttcc gtcgtttggc aatctcatcc atacctcccg accagcgcag tcaacaaacc
     2881 catcattaaa tttacaactc ataatcccgc cgagttggct tttttaatag ctactaaagc
     2941 tcactctcct tttctctact gccacacgga aggactcgtg cggtagcaga aaaatcaaga
     3001 atttgcacaa gggttgagta cttgtttgga tttgtaggaa acagcatgaa cggttcgttt
     3061 atacgcacta ttggaatttc aagggctaaa gcaaaaatag ggatgatgaa cctgacgtac
     3121 aatattttcc gctgtactca attaaaaata gtggttgtaa tgggataggt atgaccggac
     3181 ataaaaaata atttagtatg taactgatag ttaattaaat aaaaaataag aagagtaata
     3241 tttgattttt tcaaaatcaa agaataaatt tgtttcgagc attagcacaa aaaaagtagt
     3301 ttttcgaggt gccctttagt aatgggagtt ttgaacgctc tggttgagag gtatagataa
     3361 tcttgccata cgactaaagt cgtgcggcag gaagggggaa aaatgccctc aatttcttag
     3421 tgtctatatt gtggccgtta gggtcaattt gacatagatg ctcggaaaaa ttgaaccact
     3481 gtttggctta gaaaaacata ccgatgtgac tacacgaaaa tttaaataaa gggtgaagaa
     3541 gttgacccga acacacctga aaccaaagta gggcaaaatt aaaaacaaat caaaatgaaa
     3601 aagttattaa ttttattatg ttttttgggt tttgttaacc tgactctttc tgctcaaaac
     3661 atctacattc gaggccacct tcaggttatc tatattgggc ctgagggaat ggatgttatt
     3721 tgcaaccctc cgtatgatag agattgcgta tcgattatat ctgagcctgg actaccagct
     3781 aaagctacag ctgaggtcag cgataaagga gttacaaaga ttcaacccag agttcaagtt
     3841 gtgggagaaa cactaaattc caacggttta ccaacaaaaa tatatcgcat acggaaagat
     3901 gccgtaaaag aggttgttaa aaactagatt gctaacttaa ggggtggtat ccccatccct
     3961 tattttatac caaaccatgc gaaacatact aacaacattt gtctttttat gcttatcgtt
     4021 accggcaatc agcagggttg gaggggttga aattttcttt gccattaaac cctcggactg
     4081 catatcgtgc atggctccca tcagggatat acttgccgaa attgagaaac aggagctcat
     4141 ggaacaggtt acctttatcg taagggatgt tcctgaaaag gcgcttccca agtacactaa
     4201 ggaggtttta aagataccca acagtataaa tgtggtttca tcggaaccac ttttcagcaa
     4261 gtactccttt tacaacgctt ctgcaattac ggtgattgat aactccactg tgcctataca
     4321 gcgaatcacg ttcccccttg cttactattt tgttaataaa accatgttcg ttgagcgatt
     4381 atccaaaagt aacaaaacga ttgtagcact ggaaagagta aaacttaaag agtcgctggg
     4441 tggaatcaga atgttgtcca tatcaaacga ttcgttgctt tttataaatg acaggctgtt
     4501 taacaccatt tcgctgtttt cagctggcag cggaaaacta gtcggaaatt ttacgctaga
     4561 cagcgcttgg tacgcggcac cctatagtaa ggataccttt aagctaagac agatatggat
     4621 gaatcagcat gtacttgaaa gggcatctta cccccagtac tattttaaag gagttttcaa
     4681 ccatagaggt agaactataa tttctgcaat atgctactac ccgttcacaa ctggtaaaga
     4741 cttttttata ctacccaaaa accttttttt tatgctggac acactattga tgcccataag
     4801 ctacaggtgg ctttacattg ataaggaaac cccgatagtt aatttttata atgctgtatt
     4861 ttttcaagat gataacctca tcaccaccac ctttgatgtt gtcaacaacc ggctaaactt
     4921 taaccccgat tttttatact attttaaacc cgaaggcgac tcgttgattt ttgataaatc
     4981 cctacatgta gagccaccaa gttttatcaa gaatggatat tctatgaatg gtttttcatt
     5041 gaactacata tctgagtata tgggcaatat taccgtttgg ttctcctact accctgagtt
     5101 cttcgaccta aggttggaga agtgggtatc aatagataca ggctacgttg attctaaaat
     5161 atcaactaac acctttgaac agggtcagcc tgttaatttt caagtgctgg gcgtatgggc
     5221 tgaagcaaac cagtacagca tgattctaag gtttggcctg caagtttact acatggttca
     5281 agctgataag aacataattc aaagcgctct aatctatacc cttagcgaag ggaatcaact
     5341 ccaaatggtt cgaaagagta agaatgagtt cgttggaact gattacaact caaaaacggg
     5401 agatactgag attatcaaat tcacaattaa tggggtgcaa acatttattt caccctcgct
     5461 caacgaagcc tgtgccttcg tcgtacctat gtagcgttct ctgacctcat aagatactat
     5521 cagtaagctt tccattgggg taaatataaa caatttcaag cggtttaact ttcgttcggt
     5581 taaatttgtg gacatcagcc cactctgtta attcgggctt tgccaggtaa atatagctaa
     5641 ttgtaagttt tccagcgtat gtcatttcaa aaatagcagg cgcagccagc tactgatagc
     5701 cctgcggccg cagaaccacc gccgaaagcg tttagattat ttcaacctgt aacatcaggc
     5761 gggctgaggc taggttagtg tttcccttca ctaatccagc ccaaccttca gggggttaac
     5821 ttacgttgcc ttaccgttta accgttggaa ttaccggtta cggggctgaa ttagtagacg
     5881 gccagccagg tttagggcgg ccaggactgc tgcggtttga acaacaccca gtagcggaat
     5941 tagcgctagt gtaaccacaa ccacaccaag ggttgacccc cacaggtcgg cagagtaact
     6001 cattgccgcg gggtttgccc tatcggcaac tgcactcgtc catagcacat actggtagcc
     6061 aacaaggact gatggaataa gggtgaagag ggaaataacc agcagcaaca gccacactgc
     6121 agcgacatca cgtagcatcc atatcagcgg aattagcgca aagtagcccg ccatggcaat
     6181 ggtcaggctt ccaaccgaaa gccttatgaa tctgggaaga acgtaggcgc caacagccaa
     6241 tccggccata aagagcgcca cgataagccc gatacccgca tacaaaaaac caaagagcac
     6301 ctgaaacatc aggatgatta tcatttcggc cgaggccccc gaaaacccgg caaggaacat
     6361 tccccttagc ggtttcccaa gaaaaaaaac aggtagcaaa actacagcta agcctaccag
     6421 catccacacc aaatggttgc tgccaaactg ccccaggtag ctcatggtga aaaggaaaac
     6481 cgggtaggga tggttgtcgg tgtttagcct g
//
LOCUS       DAOSHM010000095         6466 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_10460_length_6466_cov_21.880362, whole genome shotgun
            sequence.
ACCESSION   DAOSHM010000095 DAOSHM010000000
VERSION     DAOSHM010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 6466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6466
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10460_length_6466_cov_21.880362"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..998)
                     /locus_tag="PK839_09960"
     CDS             complement(<1..998)
                     /locus_tag="PK839_09960"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOG73098.1"
                     /translation="MPSIYRLLKLNRLVRSNRLKLLLIFMAQLLNRRYYSVRIDPVFA
                     CNLRCRMCYFSRSRKPNPEKFSPDELATIAGKLFGNALQVVVGCGAEPTMYAHFVDIV
                     KKASEYSVPHISLVTNGQLLTRDHLNELAESGLNELVVSVHGASQQTYEHLMVGASWE
                     KLHKLLNSVNEMRAVNRYPNFTLRVNYTVNPLNLHELETFFDAFGQYQINTLQIRPVM
                     NIGGEYEDGFSQSDIHTYSKIIAKVRNYCRERGITLLANTDDPTYQRSSKVSWVLNET
                     YKYVSPAMVVSSDFKWKEETLSAYLRRTGWYATMLKRIFWFQRAEKPDDLAQKYSAR"
     gene            complement(1347..2498)
                     /locus_tag="PK839_09965"
     CDS             complement(1347..2498)
                     /locus_tag="PK839_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HOG73099.1"
                     /translation="MADTTYIFLQQYWWFIVSLLGALLVFLLFVQGGQTLIYTLGKNN
                     TERTMLVNVLGRKWEFTFTTLVTFGGGFFASFPLFYSTSFGGAYWVWMAILFCFTLQA
                     ISYEFRTKPNNFLGQKTYEVFLFLNGLLGTILLGTAVATFFTGSQFSVNKLNLVSSGN
                     PVISSWETPWHGLEAALNFHNLSLGLAVFFLARTLAILYFMNSVNHDVILERSRKHLL
                     YNAIPFLVFFLTFVIWLMVRDGFAVNPSNGAVFMEKYKYLHNLLQMPAVLVLFLVGVV
                     LVLWGIGKSLLSKYSKGIWFAGFGTVFTVLALFLIAGYNNTAFYPSTFNIQHSLTIYN
                     SSSSKFTLTVMSIVSILIPFVVAYIWYAWKSLNVKKIDEDEIKTESHLY"
     gene            complement(2501..4063)
                     /locus_tag="PK839_09970"
     CDS             complement(2501..4063)
                     /locus_tag="PK839_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HOG73100.1"
                     /translation="MEQFDLSLVNWSRAQFALTAMYHWLFVPLTLGLSFIIAFMETLY
                     YKTGDEEWKRITKFWMTLFGINFAIGVATGLILEFEFGTNWSNYSWFVGDIFGAPLAI
                     EGIMAFFMEATFIAVMFFGWNKVSKKFHLVSTWLVALGSNLSALWILVANAWMQYPVG
                     MHFNPDTARNEMHNFWEVLLSPVAVNKFLHSITSGYVLSAIFVIGVCAWFMLRGRHQL
                     MVKRSVTIASVFGLVTSLFLLVTGDGSAYQVAQKQPMKLAVMEGLYEGKEGAPLTAIG
                     ILTPGKQYDDSKDPYIFKIQIPRLLSWLGYRSGDAFVPGVKDVIEGGYVYRDGNGNER
                     VAPSVEQKMAKGKIAIGALAEYKKAAEAGDSSAMAYHKKLLDENFEYFGYGYLNDPKS
                     VIPNIPLTFYSFHVMVALGFLFIIFFIVMLYLVLFRQFKVKRWLLYLSIVMIPLAFLA
                     GQAGWIVTEVGRQPWVIQDVLPTVAAISRIDTSAVQITFFLFLVIFTALLIAELRIMF
                     KQIKIGPKEGGK"
     gene            complement(4070..4285)
                     /locus_tag="PK839_09975"
     CDS             complement(4070..4285)
                     /locus_tag="PK839_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015547662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4492 domain-containing protein"
                     /protein_id="HOG73101.1"
                     /translation="MELPGKIFRFYYEGFRSMTVGKTLWVIILIKLFVMFAILKIFFF
                     PDILKKNFKTDTERSKYVIEQLTSNQK"
     gene            complement(4378..5361)
                     /locus_tag="PK839_09980"
     CDS             complement(4378..5361)
                     /locus_tag="PK839_09980"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF020459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GSCFA domain-containing protein"
                     /protein_id="HOG73102.1"
                     /translation="MEFRTEVAVDDFPFRLGYATPTLFCGSCFTENIGGIMRTLKMPV
                     LVNPFGVVYNPLSVAQVLQRSVAGKIYNADELQFRDGLWFSFDFHSRFASPNRNACIA
                     LLNEAVSSAKDFISTTQCIVVTFGTARVYKLKGSGEVVSNCHKVPAVEFENKLLAVDE
                     VVEIWSNLISMLLGLNPSLKLVLTVSPIRHWKDGAPGNQLSKSTLIVAVHRLVEMYSD
                     NVFYFPSYELMMDDLRDYRFYDDDMLHPSPKAIEYIWSKFSRVLIDDDSMKLAAQIQK
                     IIQAAGHRPFNTDTPEHKRFVDKILKSIQDIKLIHPSINFEREIAILKGEQ"
     gene            complement(5351..6217)
                     /locus_tag="PK839_09985"
     CDS             complement(5351..6217)
                     /locus_tag="PK839_09985"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HOG73103.1"
                     /translation="MKNSTALINVESVHQEWLKTNGYRLFTWYIQPSSPKAAVLLVHG
                     LGEHSMRYQTWAKQFADAGFAFYCWDHVGHGQSSGQRGCVPNYEHLLQEVDFIINKFN
                     TQIGSIPLVLYGHSMGGNIALNYAIRRPSKINLLVVTSPWLKLVSPLNPALKSLVKLL
                     DLCLPFLPMNSGIRPEQVSHEMKVVQEYATDPLIHRRITPRLYLAMERAAEYAMNHSE
                     QITIPTLLMHGSSDTLLSSMATSEIAAKMHHSTYVEWDELYHELHNETKSNEVFKTIV
                     NWLNVKIAQYGV"
BASE COUNT         1862 a         1465 c         1480 g         1659 t
ORIGIN      
        1 cgggcgctat acttttgggc cagatcatca ggtttttcag cacgctgaaa ccaaaaaatt
       61 ctcttaagca tggtggcata ccatccggtt cttcgcaggt aggccgaaag tgtctcttcc
      121 ttccacttaa aatcgctact aaccaccatt gccggagaaa cgtacttgta ggtttcgttc
      181 agaacccagc tgactttaga cgaccgctgg taggtgggat catcggtgtt ggccagtagg
      241 gtgatgcccc gctcccgaca ataattccgg actttggcaa taattttgct gtaggtgtga
      301 atgtcgcttt gggagaaccc atcttcatac tcgcctccga tgttcatcac cggacgaatc
      361 tgaagggtgt taatttggta ttgtccaaaa gcatcgaaaa aagtttccag ctcatgcagg
      421 tttaacgggt taactgtata gttaactcga agggtaaagt tggggtagcg atttaccgct
      481 ctcatctcgt taaccgagtt caaaagcttg tgtagctttt cccagctagc tccaaccata
      541 agatgctcgt aggtttgctg gctagcccca tgaaccgaca caaccagctc gttgaggccc
      601 gattcggcaa gctcgttcaa atggtcccgg gtaagtagtt ggccgttagt taccaagctg
      661 atgtggggca ctgagtactc actggccttt ttaacaatat ccacaaaatg tgcatacatc
      721 gttggctcgg caccacaacc caccacaacc tgcagcgcat tgccaaatag cttccctgca
      781 atagtagcca gctcatcggg tgaaaatttt tcgggattcg gttttcgaga ccggctaaaa
      841 tagcacatgc ggcacctcag gttgcaggca aaaaccggat caatacgaac cgagtagtac
      901 ctacggttca gcagctgtgc catgaagatt agcagaagct taaggcggtt acttctaacc
      961 agcctgttga gcttcagcaa cctgtatata ctaggcatta tccgaaggat gtttcctctc
     1021 tgtacgccta attgcccata gggacaacag gtaggttagg taaatcaaca ccggccagct
     1081 taacagccat aaaattccgg aaaggtattt catcggaaaa tatttattag gttaattgct
     1141 ataggttaca attttatctt ttcgtttcct cctacccaaa aagctcatca cccaacatat
     1201 ttcatccgta gttgaatctt cctggcttat aaaccacagc tgagtgctag agatgccacc
     1261 tgaatgtttt acttgccttg atattcaaag taaagatgaa cgggtagata ccatagaaac
     1321 atcattgttt aaagtaaagt ccaaagttag tagagatgcg attcggtctt aatttcgtcc
     1381 tcatcaattt ttttcacatt caagcttttc caggcgtacc agatgtaagc caccacaaag
     1441 ggaataagta tggaaacaat gctcatcacc gtaagggtga acttgctcga cgagctgttg
     1501 tagatggtaa gagaatgctg gatattgaaa gttgaagggt aaaaggcggt gttgttgtag
     1561 cctgcaatca ggaaaagagc caggacggtg aaaacggttc caaaaccggc aaaccaaata
     1621 ccttttgaat atttggaaag aagcgactta ccaattcccc aaagcaccag caccacgcca
     1681 acaaggaaaa gaaccagcac agctggcatt tgcaacaagt tatgcaggta cttgtatttc
     1741 tccatgaaaa cagcgccgtt tgaaggattt acagcaaatc catcccttac cataagccaa
     1801 attacaaagg tgaggaagaa taccagaaaa gggatggcgt tgtagagcag gtgttttcgg
     1861 gagcgttcaa ggatgacatc atggttaacg ctgttcatga agtataggat tgccagcgtt
     1921 cgggccagaa agaaaacagc cagccccaac gaaaggttat gaaagtttag agcagcctcg
     1981 agtccatgcc atggggtttc ccaggacgag attacggggt tgcccgagct gaccaggttg
     2041 agcttgttta ccgagaattg cgatccggta aagaaggtgg ccacggctgt gcccaaaagg
     2101 attgtaccca gcagcccgtt aaggaaaagg aacacttcgt atgttttctg ccccagaaag
     2161 ttgttgggtt tggtccggaa ttcataggaa atagcttgaa gggtgaagca gaatagaatg
     2221 gccatccaaa cccagtaggc acctccaaag ctggtagagt agaatagcgg aaacgatgca
     2281 aaaaaacctc caccaaaggt aaccagcgtg gtgaaagtaa actcccactt gcggcccagc
     2341 acattcacca gcatggttcg ctcggtattg ttcttaccaa gggtataaat caaggtttgt
     2401 ccaccctgaa caaatagcaa gaatactagt aaggcaccta aaagcgaaac gatgaaccac
     2461 cagtattgct gcaggaatat gtaggttgtg tcggccatgg ctattttcct ccctctttag
     2521 gtcctatttt aatttgcttg aacataattc gaagttcggc gatgagcagt gcggtaaaga
     2581 ttaccaggaa caggaaaaag gtaatctgaa cggcgctggt atcaatcctt gagattgctg
     2641 caaccgtggg gagcacatcc tgtatgaccc agggttggcg ccctacttcg gtaacaatcc
     2701 aaccggcctg accggcaaga aaagctagcg gaatcatcac aattgagagg tagagaagcc
     2761 accgcttaac cttgaattgc cgaaatagca ccaggtagag catgacaatg aagaaaataa
     2821 taaaaaggaa tcccagcgcc accataacat ggaagctgta gaaggtaagg ggaatattgg
     2881 gtattacgct tttgggatcg ttgaggtagc cgtagccgaa gtactcaaaa ttttcgtcaa
     2941 gcagcttctt atggtaagcc attgcagagg aatctccggc ttctgcggcc ttcttatatt
     3001 ctgctaaggc acctatggcg atttttccct tggccatttt ctgctcaacc gatggggcta
     3061 cccgttcatt cccattacca tccctgtaaa catatccacc ttcaataacg tcctttactc
     3121 cgggaacaaa ggcatcgccg gaacggtatc ccaaccatga aagaagccgt ggaatctgaa
     3181 ttttgaagat gtaggggtcc tttgaatcgt catactgctt gccgggggta agtatgccaa
     3241 tggcagtgag aggtgcaccc tctttgccct cgtagagccc ttccatcacc gccagcttca
     3301 tgggctgctt ctgggcaacc tggtatgccg atccatcacc tgttaccagt aaaaacaggg
     3361 atgttaccag tccaaacacc gaagcaatgg taacgcttcg tttaaccata agctggtgac
     3421 gacccctgag cataaaccat gcgcatacac caatcacaaa aattgcggat agcacatacc
     3481 ccgaggtgat ggagtgcaaa aatttgttaa ccgcaaccgg cgagagcagc acctcccaaa
     3541 agttgtgcat ctcgttacgg gcagtatcgg ggttaaagtg catgcccaca ggatactgca
     3601 tccaggcgtt ggccacaaga atccataagg ccgatagatt tgaacccagc gctaccagcc
     3661 acgtagatac cagatggaac ttcttgctaa ccttgttcca cccgaaaaac atcacagcaa
     3721 taaaagtggc ctccataaaa aaggccataa ttccctcaat agccagcgga gcaccaaaaa
     3781 tatcgcctac aaaccacgag tagttgctcc agttggtacc aaactcaaat tccagaataa
     3841 ggcctgttgc caccccaatg gcaaagttga tgccgaagag ggtcatccag aacttggtga
     3901 tccgtttcca ctcctcgtcg ccggttttgt agtatagcgt ttccatgaac gctatgataa
     3961 acgataagcc taaggtaagc ggtacaaata gccagtggta cattgccgtt agcgcaaact
     4021 gggctctgga ccagttgaca agtgataaat cgaattgctc catagtcagc tatttttggt
     4081 tactggttag ttgctctatt acgtacttgc tgcgttcagt atccgtttta aagttcttct
     4141 ttaagatatc gggaaagaaa aagatcttaa gaatggcaaa cattacgaag agtttaatca
     4201 gaataatgac ccacaatgtt ttccccacgg tcatactcct gaatccctcg tagtaaaatc
     4261 ggaaaatttt acccggtagc tccatggtca aaataagtta tgatttttca aaattaacaa
     4321 aaattaacac gaatggttgg ctgtcccgat tgaatttaac aaagttatct ggtagaacta
     4381 ttgctcgcct ttaagaatgg ctatttccct ttcaaaattg atggaaggat gaatgagctt
     4441 gatgtcctgg attgatttta aaattttgtc aacaaacctt ttatgttcgg gtgtatcggt
     4501 gttaaacggc cgatgtcccg ctgcctggat tattttttga atttgggctg ccaacttcat
     4561 ggaatcgtca tcaattagaa cccttgaaaa cttactccaa atgtactcaa tagcttttgg
     4621 tgaaggatga agcatatcgt cgtcgtaaaa cctgtagtcg cgcaaatcgt ccatcatcag
     4681 ctcatagcta gggaagtaaa aaacgttatc gctgtacatt tcaaccaacc tgtggactgc
     4741 aacaattagg gttgatttgc tcagctgatt accgggtgcc ccatccttcc agtgcctgat
     4801 tgggctaaca gttagcacta actttaggct ggggtttaac cccaaaagca tggaaatcag
     4861 gttgctccat atttccacaa cctcatcaac agccaagagc ttattctcaa actcaacagc
     4921 tggaacttta tggcagttgg ataccacctc gccactaccc tttaacttgt aaaccctggc
     4981 ggtgccgaat gtaacaacaa tgcactgggt tgtggaaata aaatctttgg ctgaactgac
     5041 cgcttcgttt agcagggcaa tacaggcatt gcggttagga gaggcaaatc gggaatggaa
     5101 atcaaaacta aaccacaagc catctcggaa ttgcaactcg tcagcattgt agattttacc
     5161 tgccactgag cgttgcaaaa cctgcgcaac ggataaggga ttgtaaacca caccaaaggg
     5221 gttaaccaga acgggcattt tcaacgttcg catgatgccg ccaatgtttt ccgtaaagca
     5281 tgaaccgcaa aaaagggttg gtgtggcata gcccagtcta aaaggaaaat catcaaccgc
     5341 tacctcagtt ctaaactcca tattgggcaa tttttacatt caaccaattt acaatcgttt
     5401 taaaaacttc attgctttta gtttcattgt gaagctcatg gtatagctcg tcccattcca
     5461 cataggtgga gtggtgcatt tttgcggcaa tctcgctagt ggccattgac gagagcagag
     5521 tgtcggaact accgtgcatc agaagtgtgg ggatggtaat ttgttcggaa tggttcatgg
     5581 catactcagc ggccctctcc atggcaaggt agagccgagg ggtaattcgc cgatgaataa
     5641 gcggatccgt ggcatactcc tgtaccacct tcatttcatg cgatacctgt tcgggtcgta
     5701 ttccagagtt catgggtaga aaaggtaagc ataaatctaa gagcttaacc agacttttta
     5761 acgcagggtt gagtggactt accagcttca accagggtga agtaacaacc agcaggttga
     5821 ttttggatgg tcttcttatt gcgtagttca gcgcaatgtt gccccccatg ctatggccgt
     5881 aaagcactaa gggaattgaa ccaatttgcg tgttgaattt atttatgatg aaatctactt
     5941 cttgcaggag gtgctcgtag tttggaacac atccgcgttg accgcttgat tgcccatgac
     6001 caacatgatc ccagcagtag aacgcgaaac ctgcatcagc gaattgtttt gcccaggtct
     6061 ggtagcgcat ggaatgctcc ccaagcccat gaacaagtag tactgcagcc ttagggctgg
     6121 atggttgaat ataccatgta aacagcctat aaccgttagt ttttaaccat tcctgatgaa
     6181 cagattccac gttgattagc gcagttgaat ttttcaaact gcaatatagc taaaaacgtg
     6241 aattgtttgc aggaagagtt agtaaagtta aagcagaaag ggaacaataa taaggaatgc
     6301 ttgtgttgaa gaatgagatg tatgctggca attttgagag gttgaaaacc agccttttac
     6361 ctgtcaaagg aactctccct gaacttgtcg aagggtggaa taagccagca ggatgagaag
     6421 tttattttat cctttatcat ttctcatctg aaatccgccg gtaggc
//
LOCUS       DAOSHM010000096         6424 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_10574_length_6424_cov_21.574030, whole genome shotgun
            sequence.
ACCESSION   DAOSHM010000096 DAOSHM010000000
VERSION     DAOSHM010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 6424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6424
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10574_length_6424_cov_21.574030"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            185..1456
                     /locus_tag="PK839_09990"
     CDS             185..1456
                     /locus_tag="PK839_09990"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOG73104.1"
                     /translation="MMKVLAGFFRKSLKYSAMVIALLISASAWAQEKDYITVNGVVKD
                     SKTKERLVFSGIYVPGTSIGTVANSEGQFTLKIPKTLNVQEFGISHLGYHNGIFPVSD
                     GNGRRENVYYLDPASVEIKGVVVRPKDPRTLILQALNRIEDNYSRVPNTLTGFYRETI
                     KQRRDYVSIAEAVTEIYKSPYKFTLESDRIKVIKGRKGDNVKKADTLIVKMQGGPNVS
                     LYLDIVRYPELILGLEEMKHYQYELTDMVSIDNRSNYVISFKPLAKLEYPLYYGKIYV
                     DVESLAITMAEFSMDISDVEKATQAFVRKKPAGLIFEPVSTSYLVTYKKLGDRYYLNY
                     MRSEIKFQADWKKKIFKTSYTLMSEMAITERDTVNAAKIAAKESFKPYAILTDKVNEY
                     FDEDYWGSYNTIEPDESIQSAIAKYNRRFKR"
     gene            1543..2088
                     /locus_tag="PK839_09995"
     CDS             1543..2088
                     /locus_tag="PK839_09995"
                     /inference="COORDINATES: protein motif:HMM:TIGR02985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HOG73105.1"
                     /translation="MSLNGLNRQSRIKDDSIGDFEVLFKTYYVPLCRFAFSIVRDSDQ
                     AEEIVQEFFYNYWKNRKNIGIKFSIRAYMYKAVKNSALRYLAHRKVESSYGVTLGSTS
                     GGFDNLDESSDELLKIVEATLQQLPERCRQAFILSRRDGLKYKEIAAKLSVSEKTVEA
                     DMTKALMALRQNLSRLGFNTH"
     gene            2137..3144
                     /locus_tag="PK839_10000"
     CDS             2137..3144
                     /locus_tag="PK839_10000"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HOG73106.1"
                     /translation="MINSTPNTQDYKKISRYLCDDMLFPERLIFLALVACKPRLRKNF
                     KAARMDMAMADKLISAGSYNPNVAWEKLHDRLENESLIPDEIRNTVTFYKGAYLKYAA
                     IAALAILLIGIPGYFLFSPSRIVVENGRDGSTMITSLPDGTSVYLAENSTLSYPKHFK
                     GKLRSVTLNGEAFFEVAPNPHKPFIVNTKAATVRVLGTTFNIKSTDSNNFELCVVEGK
                     VKVSSLGGDVLATAGERVYVRNSLLQKSISAGQPTVKHRMTRLQFQDERMEDIVGIIN
                     RTYGSNIELMGDNLRNRRISVTFENELSSIVSILSASFNLQIETEPDGTIVLSEKSVS
                     P"
     gene            3104..4699
                     /locus_tag="PK839_10005"
     CDS             3104..4699
                     /locus_tag="PK839_10005"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOG73107.1"
                     /translation="MAPLFSAKNQLARKANRLFRAIAYRVALLILFAGIGINGHADEA
                     DTLLTKPITISLPAENLYNALNHIGERAGCFFIYDSRDVRGSKRIKPVTWVNKPMYEL
                     LNLLVADSLVSYRVIDRHILIYRKSKDAHSGDSTRRESYLQVGGRLYDGETRQVITFA
                     TVTLNEIGLGTITNNDGAFTMRIPSSFIHASVVISHLGYQSQTIPIELLLGKKYTIYL
                     QPRYIPIQEVIIRNIDARGIVTAMVENRYKNYASNDVYLTGFYREGVSRNNQYLGYSE
                     AIFRIYKSAITESTSSDQVKLLKARKLEKQDRSDTLTVKLMAGIKGTLEMDIAKSLPD
                     FIDPEYMDSYTYSKSDIVAHDSRTAYAISFEQKKGVELPLYMGTLYVDVENLALLAAD
                     FELNPKYIEKAASSLIVRKNRSVKVKPQQVSYSVSYRELNGVYYVQHIRGDISVRISS
                     RYRLFTDSYNLFFEFVNMQIDTLNVQRFSRRDVVKPYTVFSDGDFAYDASFWGDMNFI
                     EPEEDVARAIARINPKIESVVGE"
     gene            complement(4817..5836)
                     /locus_tag="PK839_10010"
     CDS             complement(4817..5836)
                     /locus_tag="PK839_10010"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007210510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HOG73108.1"
                     /translation="MQNLDWKNLPFGYLKTDYNVRCRYSDGKWGNLEVSDSEYINIHM
                     SATALHYGQEGFEGMKAYRGKDGRIRIFRWEENAKRLQLTADGILMAPVPMELFHEAV
                     VKAVKLNEKYVPPYGTGASLYIRPLLFGSGAEIGVKPAKEYVFIVFVTPVGPYFKEGF
                     NPTKIAIIRDSDRAAPLGTGTFKIGGNYAASLRGTIKAHNAGYSSPMYLDACGKRYID
                     EIGAANFFAIKNNTYITPKSTSILNSITNMSVMQLAQDLGLKVERRPVAVEELETFEE
                     VGACGTAAVISPIGEINDIDGGKVYKFCKDGKAGPISTKLYQTLTGIQYGDIEDKHGW
                     VSFVE"
BASE COUNT         1847 a         1422 c         1458 g         1697 t
ORIGIN      
        1 cggacaagtt cttgaatctt gaccttaact aaacgacatt gaattaaaat ttatataata
       61 atccattttt tatagggtaa atgcctttcc gggtatcata tatgcaaagg attaatttta
      121 cttgattaat gtaagctgat cgaacaaaaa atcattgaag ttaaaaccct ataaaccagc
      181 aagaatgatg aaagtgctag caggattctt cagaaaaagt ctgaaataca gcgcaatggt
      241 tattgcactt cttatttcag caagcgcttg ggcacaggaa aaagactaca tcaccgttaa
      301 cggtgtggta aaggattcaa aaacgaagga gcgattggtg ttttccggaa tatatgttcc
      361 cggaaccagc atcggaacag tggccaactc cgaagggcag tttaccctga aaattcctaa
      421 aacgttgaat gtccaggaat ttggaatttc ccacctgggc taccataatg gtattttccc
      481 ggtttccgat gggaacgggc gacgtgaaaa tgtgtactac cttgatcccg cctcggttga
      541 gattaagggt gtagttgttc ggcccaaaga cccacggact ttaatcttgc aggccctcaa
      601 ccggatagag gacaactaca gcagggttcc caataccctg accggtttct atcgcgaaac
      661 cattaagcag cgtcgcgact atgtgtccat tgccgaagct gttaccgaaa tctacaaatc
      721 accctataag tttaccctgg aatcggatag gattaaggtt atcaaggggc gtaaaggcga
      781 taacgtgaaa aaggccgata cccttatcgt gaaaatgcag ggtggcccca atgtttccct
      841 ttatctggat atagtcaggt accccgagct tatactggga ttggaggaaa tgaagcacta
      901 ccaatacgag cttaccgata tggtcagcat cgacaaccgt tccaactatg ttatttcatt
      961 taagccactt gctaagctcg aatacccgct ttactacggt aagatatacg ttgacgtgga
     1021 gtcgctggcc atcaccatgg ccgagtttag catggatatt tcggatgttg aaaaggctac
     1081 ccaggcattt gtcaggaaaa aacctgccgg gctgatcttc gaacccgtca gcaccagcta
     1141 cctggttacc tacaaaaagt tgggcgacag gtactaccta aactacatgc gcagcgaaat
     1201 taaatttcaa gccgactgga aaaagaagat ttttaaaacc agctacaccc tcatgtccga
     1261 aatggccatt accgaacgtg acaccgttaa tgctgccaag attgctgcaa aggaatcgtt
     1321 taaaccttat gccattttaa ccgacaaggt aaacgagtac tttgacgagg actactgggg
     1381 ctcatataac accattgagc ctgatgaatc cattcaatcg gctattgcca agtacaaccg
     1441 taggtttaaa aggtaaccct atgggcatct tagcctacct attgtttctg cccgtagcca
     1501 attattttat acctttggac taataccgta gcctgattat agatgagcct gaacgggttg
     1561 aaccggcaaa gcaggattaa ggatgatagc attggtgatt ttgaggttct cttcaaaacc
     1621 tactatgtcc cgctttgccg gtttgccttt tcaatcgtca gagattccga tcaagccgaa
     1681 gaaattgttc aggaattttt ttacaactac tggaaaaacc gtaaaaatat cggcatcaag
     1741 ttttcaatca gggcatatat gtacaaagct gttaagaata gcgcattgcg atacctggca
     1801 catagaaagg tggagagcag ctatggtgtt accttaggta gtacatctgg aggttttgat
     1861 aatcttgatg agagctccga cgagctgtta aaaattgttg aggccacact tcaacagctg
     1921 cccgaaagat gccgtcaggc atttatactt agccgtaggg atgggcttaa atacaaggaa
     1981 attgcagcaa aactttcggt ttcggaaaaa acggttgagg ctgatatgac aaaagcgctg
     2041 atggctctca ggcagaacct gtcaagattg ggttttaata cccattaaag gaggcaaagg
     2101 ttttcaaaac aactattttt atttttatag ataacgatga tcaatagcac accaaatacc
     2161 caggattaca aaaaaatatc ccgctaccta tgcgacgaca tgctttttcc cgagaggttg
     2221 attttccttg ctctggtagc ctgcaagcct cggttaagga agaattttaa ggctgcaagg
     2281 atggacatgg cgatggcgga taagcttatt agtgcaggat catataaccc caacgtggcg
     2341 tgggaaaaat tgcacgatag gttggaaaac gaaagcctta tccctgatga aataaggaat
     2401 acagtgactt tctacaaggg agcatacttg aaatatgcag ccattgctgc tttggccata
     2461 ttactcattg gaatacccgg ctactttctt ttttcgccct caagaattgt ggttgaaaat
     2521 gggagagatg gaagcacaat gattaccagt cttcccgatg gaactagcgt ttacctggcc
     2581 gaaaactcca ccctgagcta ccccaagcac tttaaaggca aattacgaag cgttaccctt
     2641 aatggtgagg cattttttga ggttgctcca aacccccata aaccctttat tgtgaacacg
     2701 aaggctgcca ctgtcagggt tttgggaacc accttcaaca tcaaatcaac tgatagcaat
     2761 aattttgaac tttgtgtggt ggagggaaag gtaaaagtga gcagcttggg cggtgacgtg
     2821 ctggctacag ccggtgaacg ggtttatgtg aggaatagct tgcttcaaaa atccatctct
     2881 gcaggacagc caaccgtaaa gcatagaatg acacgacttc agtttcagga tgaaaggatg
     2941 gaggatattg taggaataat caatagaaca tacgggtcaa acattgagtt aatgggcgac
     3001 aatctgagaa ataggagaat ttctgttaca tttgaaaatg agcttagcag cattgtctcc
     3061 attctttcgg cctcctttaa cctgcaaata gaaacagaac ccgatggcac cattgttctc
     3121 agcgaaaaat cagttagccc gtaaggcaaa caggctgttc agggcaatcg catatcgagt
     3181 tgcgcttcta atcctttttg caggtatcgg gatcaacggc catgctgatg aagccgatac
     3241 gctgctgacc aagccaataa ccatatcgct tccggccgaa aatctttaca atgccctgaa
     3301 ccacattggc gaaagggcag gttgtttttt catttacgac agccgcgatg tgcggggaag
     3361 taagcgtatc aagccggtaa cctgggtaaa caaacctatg tatgagctgc tgaacctcct
     3421 ggttgccgat tcgctggtaa gctacagggt aattgacagg catattctga tttacaggaa
     3481 atcaaaagac gctcattccg gcgattcaac ccggcgcgaa agctacctgc aggtgggtgg
     3541 aaggctgtac gacggggaaa cccgccaggt aattacgttt gcaactgtta cgctaaacga
     3601 aattggactt ggaaccataa ccaacaacga tggggctttc accatgagaa ttccctcctc
     3661 gtttattcat gccagcgtgg ttatatcaca tttaggctac cagagccaga ccattcctat
     3721 tgagctgctg cttgggaaga aatataccat ttaccttcaa ccccgataca ttcccattca
     3781 ggaggtaatt atcaggaata ttgatgctcg tggaattgtg acggccatgg ttgaaaaccg
     3841 gtacaagaac tatgcctcaa acgatgttta ccttaccgga ttttacaggg aaggggttag
     3901 ccgcaataac cagtacctcg gctattccga ggcaatattt aggatttaca aatcggccat
     3961 taccgaaagc acatccagcg atcaggtcaa gctgctaaag gcgcgtaaac tcgaaaagca
     4021 agatagaagc gataccctga ccgttaagtt gatggctggc attaagggaa ctctcgaaat
     4081 ggacattgct aaaagcctac ccgattttat tgacccggag tacatggata gctacaccta
     4141 ctccaagtcg gacattgtag cacacgatag cagaacggcc tatgccatat catttgagca
     4201 aaagaaaggt gttgaactcc ctctttacat gggaaccctt tacgttgatg tggaaaacct
     4261 tgcgctcctg gctgccgact tcgagctaaa cccgaagtat attgaaaagg cagccagcag
     4321 ccttattgtt aggaaaaatc gctccgtaaa ggtaaaaccc cagcaggtta gctactctgt
     4381 gtcctacagg gagcttaacg gagtttacta cgtgcagcat atccggggtg atatttccgt
     4441 acgcattagc tcacgctaca ggctatttac cgacagctac aacctgtttt tcgaattcgt
     4501 gaacatgcag attgacaccc ttaacgttca gcgcttttca agacgagatg ttgtaaaacc
     4561 ctacaccgtt ttctccgatg gggattttgc ctacgatgcg agtttttggg gagatatgaa
     4621 cttcattgag cccgaggagg atgtggcccg ggcaattgcc cgaatcaacc ctaaaattga
     4681 gagtgtcgta ggggagtagc tagcctcttc ggtttgcaat tacctgcatg gtattttaac
     4741 cctataaagg tataacccga taaaaaaccc tcgggagaag acccccaagg gtttttgtgc
     4801 gttactttaa tactacttat tcaacaaagc taacccatcc atgcttatct tcaatatcac
     4861 cgtactgaat tcctgtaagg gtctggtata gcttggttga tattggccct gcctttccat
     4921 ctttacagaa tttataaacc tttccgccgt caatatcgtt tatttcgcca attggcgaaa
     4981 taactgcagc agtgccgcat gctccgacct cctcaaaagt ctcaagttcc tcaacggcaa
     5041 ccggacggcg ctcaaccttc aggcctaaat cctgtgccag ctgcattacg ctcatgttgg
     5101 taatggagtt gagtatagag gttgattttg gagtaatgta agtgttgttc ttgatggcga
     5161 agaagtttgc agcaccaatt tcatctatat acctctttcc gcaggcatca aggtacattg
     5221 gggagctgta gcctgcattg tgcgctttta tggtgccacg aaggctggct gcatagttac
     5281 caccaatttt aaatgtgccg gttcccagcg gagcggctct atcgctatca cgtataatgg
     5341 ctatttttgt tgggttaaag ccctccttga agtaggggcc tacgggcgta acgaatacaa
     5401 taaaaacgta ctcctttgcc ggtttaacgc caatttcggc acccgacccg aacagcagcg
     5461 gcctgatgta tagcgatgct cctgttccgt agggtggaac atacttctcg tttagcttaa
     5521 cggcttttac taccgcctcg tgaaaaagct ccatcggcac gggtgccatc agtatgccat
     5581 cagctgttag ctgaagccgc ttggcgtttt cctcccaacg gaaaatgcga atccgaccgt
     5641 ctttaccacg ataggccttc atgccctcaa agccttcctg cccgtagtgc agggcggtag
     5701 ccgacatgtg aatgttgata tactccgaat ccgaaacctc cagattaccc catttaccgt
     5761 cgctataacg acaccttacg ttgtaatcgg ttttcaaata accaaacgga aggtttttcc
     5821 aatcaagatt ttgcatgact gcagattttt agaattgtta ttaacaatgt tgcctcacaa
     5881 aattagtaga attatattat ttctcctaac atttagtgct attatgacag ctaaatgatt
     5941 taaaagttta ataaaatatt caatatgaat taaaaattgt aactacacgg tgatggtaac
     6001 agtgcatacg acattttgag ctgatgctag tttttatctg tatggcggag attgaccggg
     6061 ttggcaatac tcgtaaggat tttttaaaac gcctcgtagt ggcgatgatt tttttatacg
     6121 agcgtggcgc attttagtgt ttactcttga gaccaccatg gaggggacgg ccttcatggc
     6181 tagcccaaca atgaaggaag ccacgttgag caaaacaata ggctaccaca ccccatgcta
     6241 ccacacgaac cagttcgcca gccggcggag agcccaacga aatttgtcat cccctatggt
     6301 gttgagtagt gcgtaagttt tagtggagag aatgaaagaa cgctgtagca gctattaaaa
     6361 agcaaatccg gaaggactat gggttttaaa tttagtaagg agaatgttga gcgccctggt
     6421 tgag
//
LOCUS       DAOSHM010000097         6403 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_10617_length_6403_cov_19.229836, whole genome shotgun
            sequence.
ACCESSION   DAOSHM010000097 DAOSHM010000000
VERSION     DAOSHM010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 6403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6403
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10617_length_6403_cov_19.229836"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1178)
                     /locus_tag="PK839_10015"
     CDS             complement(<1..1178)
                     /locus_tag="PK839_10015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG73109.1"
                     /translation="MIKQIAAGLGVLSFAVVLYSCEADWEKDYKAVTLKAPATATVSV
                     TSIADSTALLNYSLSVTGRLYVAVVPGTDETPAPDAQAILKQTVGGAVFSKQIILNDA
                     GALSSSVIVSGLVQNTSYKVFALPVNDDGVLGEIVTTEAFSTSDHHAPVLDLDSGVSP
                     AITGNASIDLNFKPVLTFDEPVVLAETFSIKMGYRNAVTLEIVWVDVPKDSITISGSK
                     VTIAQPATAMNGQYVFLSIGNGSVKDRAGNSYEGVTSGIVGGYLEGIYWRIKFSPTAT
                     QQILPADSVTSDVSMKITLDYPIKMRFPTSGEGGYVQGNVVVRYYTSTTTLDVQVPQA
                     NVAVVQDTLVQITLPRTPVYGETVTLKMAEGAMRNAYGNPSKAIAFGDKEWFISYGYT
                     "
     gene            complement(1228..2094)
                     /locus_tag="PK839_10020"
     CDS             complement(1228..2094)
                     /locus_tag="PK839_10020"
                     /inference="COORDINATES: protein motif:HMM:NF015140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HOG73110.1"
                     /translation="MKRIRYILSLAVLAVLASCNPNEMPEFNDNDAFVAFDNASMSVE
                     ENAQTLSIPVTLASIKGISASVTYTLLDGGTAVEDSNFTFADANKTLTFDATNRTQYI
                     VVNIINKPGVFTGDLRFKLQLSDDGTIKPNAENVCTITITDLDHPLAAILGTWTATGT
                     SYFNGSESWDMELTKDPSDVTVVWFYPFVNGGSGYKIYGVVNDAKTEIRVPVGQEIAP
                     PGSYGFIELKGFYGPSGEEEIPDGGYITITIEPNKLSIKDWFGSYVWGDAGQTSGLGW
                     YNIFKADVVLTR"
     gene            complement(2111..3535)
                     /locus_tag="PK839_10025"
     CDS             complement(2111..3535)
                     /locus_tag="PK839_10025"
                     /inference="COORDINATES: protein motif:HMM:NF025680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOG73111.1"
                     /translation="MKKSFIILLLTSLVLAGCENFLDEPPRLEQSSDLTLSTYSGLNN
                     SIAGAYGPLASSVWYGAFYVLDAEMRAGNAAIPTNTDFTSGRMLEAKNLNYSPTNTSG
                     LWGYAYYVISAANNVIDNLEGKDVGGVTQQDLNNLKAEALFLRALSHFDLVRLYATSM
                     AKGPDEPGVPYIYHTDQSGTAMPARETVRTVYQNIINDLLEAETIIDPAYVGAGVDPK
                     ASISLPAIQALLSRAYLYNQEWQKAADYATKVISNPAYSLWTASQLPNVWNKEIAGPG
                     GEVIFEVYEKKSNSYDAWWEGPSHMTNPIGYADCATHSDLLALYTSTDVRKTLFRTDP
                     DGVSGSFWTTKYKGKGDGDALGTPDANNVIVLRLSEMYLNRAEALVNGATIPGATALS
                     DINAIVTKRGLAPYSSVGADVVFLERRLELAFEGHLWFDYIRTNRPSTRSDKPLAADD
                     YRWLMPIPQREMDVNDNLVQNPEY"
     gene            complement(3554..>6403)
                     /locus_tag="PK839_10030"
     CDS             complement(3554..>6403)
                     /locus_tag="PK839_10030"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOG73112.1"
                     /translation="VPGTTIGVSTSNDGKYEINVPANATTLSFSFIGFTTQDVAIAGR
                     SVIDISLKPETVEMEEVVVVAYGTVKREAKTGSVTTVSSENIAEIPVSSVDKALAGKL
                     AGVQVTASSGQPGADSQIRIRGTSSINAGSEPLWVVDGIPVMGGNQSYFTNTGNAMAS
                     LNPNDIESITVLKDAAAASVYGSRAANGVILVTTKSGKEGKAKFYARSKFGISQLAND
                     NDFRVMNGEELLSFQRQAIINAGKDPDDPNGSFYRPMSLLAGEQTNWMNLFTRLGNLQ
                     EYEINASGGTNKSNFYTSLSYHKNDGVFYGIDFQKFSARVNADFKLTKTLETGARMNV
                     AYTESNDVAMQKLYYVNPAFAGMTIQPWTPAYNADGSYNINIPENSNANPRATAEYDD
                     QWEKQYRVQGTYFLTWKPIEKLEIKTNNAAEGTFGEGRRYWSPEAGGGTTGTLQSSTL
                     QYVQLTTSNTVTYNNVFAADHNVRFLVGQEAMRRTYWSYYIYSPDVDPQIPYPTTSTP
                     LVDEGSYDYNARTLMSFFGILDYNYASKYYFQGSLRYDGSSLFGSDKQWGLFWSVGGS
                     WNINKEAFMENVSFLNILKLRASYGVNGNNNIAPYQAYGVYSPSVYNGITGMLPSTPP
                     NSKLSWETNETWNLGLDFGFFKGKLNGSIDAYSRKTKDMLLSKQVPQTSGFSSNFMNI
                     GSLLNEGVEFQLDGEILKTNNLVWNAGFNISYNRTELLNLGDVKEMNYSGDSRLKHTV
                     GKSMYTFYLKDYYGVNPSTGDALWRTSDGTLTKDYNKAAFVYAGSPEPKFIGGFNTSL
                     NWKGFNLSVFLEFKQGNKVLVIERRYINSDGNQMSMNQNVSALNYWKNPGDVGVNPKP
                     VAGNSTSSYVVGSTRWLEDGSYCRIKDITLSYSLPENIVKNLKLGSVMVYVSAVNPYT
                     FHDVDFWDPERGVTGMGAGIYPMTKSFTGGIELSF"
BASE COUNT         1825 a         1452 c         1323 g         1803 t
ORIGIN      
        1 cttgtgtatc catacgagat aaaccactcc ttatcgccaa aggcaatagc cttggagggg
       61 tttccgtaag cgttacgcat ggcaccctcg gccatcttca aggtaaccgt ttcgccgtaa
      121 acgggtgttc tgggaagggt tatctgcacc agggtatctt gcacaacggc tacattagcc
      181 tgtggaacct gcacatcaag ggtagttgtt gaagtatagt accttacaac cacattgcct
      241 tgcacgtaac caccctctcc tgaagttggg aatctcatct ttatggggta atcgagggtg
      301 atcttcatgc tcacatcgct ggtcacgcta tcggcgggta gaatttgctg ggtagcggtg
      361 ggagaaaact taatgcgcca gtagataccc tccaggtaac ctccaacaat accgctggta
      421 accccctcgt agctgttacc agctctatcc ttaacagagc cattcccaat ggataaaaaa
      481 acgtactgcc cgttcatggc ggttgccggc tgggcaatgg taactttact acccgaaatg
      541 gtgatgctgt ccttaggcac atcaacccaa acaatttcta aggtaaccgc attacggtaa
      601 cccattttga tgctaaaggt ttccgcaagc accacgggct cgtcaaaggt gagaacaggt
      661 ttaaagttaa ggtcaattga agcgttgccg gtaatggcag ggctaacacc gctatctaaa
      721 tctagaaccg gggcatgatg gtcgcttgtt gaaaaggctt ctgtggtaac tatttcacca
      781 agaacgccat catcgttcac cggtaaagca aataccttgt aggaggtatt ctgcaccagc
      841 cccgaaacaa taaccgagga gcttaatgca ccagcatcat taaggataat ctgttttgaa
      901 aaaacagcgc ctccaactgt ttgcttaagt atagcctgtg catcaggagc aggagtttca
      961 tcggtaccgg gaacaacggc aacataaagc ctaccggtaa cagagagcga gtagttaagc
     1021 aaggctgtgg agtcggctat agatgttacg gaaacggtgg cagtggctgg ggctttaagg
     1081 gttacggctt tgtaatcctt ctcccaatct gcctcacaac tgtacaggac tactgcaaat
     1141 gataatactc ctagtcccgc agctatttgt ttaatcatat ttaacttcat agttgataat
     1201 ttttttcatt aagttaatgg ttctgagcta ccttgttagc acaacatcag ctttgaaaat
     1261 gttgtaccaa cctagtcctg atgtttgacc ggcatctccc cacacataag agccaaacca
     1321 atccttaata gacagcttat tgggctcaat agttatggtg atgtagccac catcaggaat
     1381 ttcttcctct cccgatgggc cataaaatcc cttaagctca ataaaaccat agctgcctgg
     1441 aggtgctatt tcctgtccaa cgggtactct tatctcggtt ttggcatcgt ttaccacgcc
     1501 ataaatctta tacccagaac ctccattgac aaatgggtag aaccaaacaa ccgtaacatc
     1561 ggaaggatct tttgtaagct ccatatccca agattcagac ccgttaaagt agctagtccc
     1621 ggtagctgtc caagtgccta aaatagccgc tagtggatgg tccagatctg taatagttat
     1681 agtgcatacg ttttcggcat taggcttgat ggttccgtcg tcggaaagct gaagcttaaa
     1741 cctaaggtcg ccggtgaaca caccaggctt attaataatg ttaaccacaa tatactgtgt
     1801 acggttggtg gcatcaaatg ttaaggtttt gttggcatca gcaaaggtaa agttactgtc
     1861 ttcaactgct gttccaccat caagtaacgt atatgttacg gaggcagata tgcccttaat
     1921 agacgcaagg gtaacaggta tacttaacgt ttgggcattt tcttcaacag acatggatgc
     1981 attgtcaaaa gccacaaaag cgtcattgtc gttaaattcc ggcatttcat ttgggttaca
     2041 cgaagcaaga actgccagaa ctgcgagtga tagaatataa cgtatccttt tcatttttat
     2101 cacttattta ttagtattca gggttttgaa ctagattgtc attaacgtcc atctctcttt
     2161 gagggatagg catcaaccat ctgtaatcat cagcagccag aggtttatcg gaacgagtag
     2221 atggcctatt tgttctgata taatcaaacc ataagtggcc ctcaaaagcg agttctaacc
     2281 gacgttcaag aaaaacaaca tcggcaccta ctgatgaata tggcgctaat cctcttttag
     2341 taacaattgc atttatgtcg cttagtgcag ttgcaccagg gatagttgca ccgttaacca
     2401 acgcctcggc tctattaagg tacatttccg aaaggcgaag aacaataacg ttgttagcat
     2461 cgggcgttcc caaagcatca ccatcgccct tacctttata cttagtagtc caaaacgaac
     2521 ctgaaacgcc atccgggtct gttctaaaaa gggtttttct aacgtcagtt gaggtatata
     2581 gtgcaagtaa atcgctatgc gtagcacaat cggcatatcc aattgggttg gtcatatgcg
     2641 aaggaccttc ccaccatgca tcgtaagaat ttgatttctt ttcgtaaacc tcaaaaatta
     2701 cctcaccacc gggaccggca atttccttat tccaaacatt aggcaactga gatgcagtcc
     2761 acaatgaata tgcaggatta ctgataacct tagtagcata atcagctgct ttctgccact
     2821 cctggttgta tagataagct ctgctcagta atgcctgaat agcaggaagg ctaatgctag
     2881 ccttaggatc gacacccgcc ccaacgtatg cagggtcaat aatggtttcg gcctcaagca
     2941 ggtcattgat aatattttgg taaacagttc tgaccgtttc ccttgcgggc attgctgtgc
     3001 cggactgatc ggtgtggtat atatatggaa ctcccggttc atcgggcccc tttgccatgg
     3061 atgtggcgta aagtcgtacc aggtcaaaat gtgaaagcgc tctcaggaat aaagcttctg
     3121 ccttcaggtt gtttaggtct tgctgcgtta ctccaccaac atcttttccc tcaaggttgt
     3181 caataacatt atttgcagcg gagataacat agtaggcata tccccataaa cctgaagtgt
     3241 tggtgggact atagttaagg ttttttgctt caagcattct accgctagta aaatcggtat
     3301 tagtaggaat ggcagcattt cctgcacgca tttctgcgtc caaaacatag aaagccccgt
     3361 accatacaga tgaagctaac gggccatatg caccggcaat ggaattattc aaacctgaat
     3421 aggttgaaag cgttaggtca ctactttgct caagccttgg gggttcgtct aggaaatttt
     3481 cacaccccgc aaggaccaag gaagtcaaca gtaatatgat gaatgatttt ttcattgctt
     3541 taatgtttat aagttaaaat gaaagttcaa taccaccggt gaatgactta gtcataggat
     3601 atataccggc acccatacca gttacaccac gttcaggatc ccaaaaatca acatcgtgga
     3661 aagtgtatgg atttacagca cttacgtaaa ccatcacgct acccaacttt aaattcttaa
     3721 caatattttc gggaagggaa tatgaaagag ttatatcctt aatacggcaa tagctgccat
     3781 cctccagcca acgagtgctg ccaaccacat aagaactagt agagtttccg gcaacaggtt
     3841 ttggatttac acccacatcg ccgggatttt tccagtagtt tagtgcagaa acattctggt
     3901 tcatggacat ttggttgcca tctgaattta tataacgtct ttcgataacc aataccttat
     3961 taccctgctt gaattcaagg aatacgctca ggttaaatcc tttccagttc agcgaagtgt
     4021 taaatcctcc aataaactta ggctcagggc taccagcgta aacaaatgca gctttgttgt
     4081 aatcttttgt aagtgtgcca tcactggttc tccataatgc atctccggtt gaggggttaa
     4141 ctccatagta atctttgagg tagaatgtgt acatgctctt accaacagta tgcttcagcc
     4201 ttgagtctcc tgagtagttc atctctttaa catctcctaa attcaagagt tcggtacggt
     4261 tatacgagat attaaatccg gcattccata ctaagttatt tgtctttaaa atttcaccat
     4321 ccagctgaaa ctcaacacct tcgtttagca acgaaccaat attcataaaa tttgaagaga
     4381 acccggatgt ttgaggaacc tgtttgctaa gcagcatatc cttagttttc ctactgtacg
     4441 catcaatgct tccatttaac tttcctttga aaaagccaaa atcaagcccg aggttccagg
     4501 tttcgttggt ttcccatgat agcttgctat taggtggcgt agagggaagc ataccggtta
     4561 tgccgttata aacactgggt gaatatacac cataggcctg gtaaggtgca atgttattat
     4621 taccattaac accgtagctg gctctaagct taagaatatt caaaaaggaa acattctcca
     4681 tgaaagcttc cttgttaatg ttccatgaac cacctactga ccagaataaa ccccactgct
     4741 tatcggaacc aaaaagcgag ctgccatcgt atctgagcga tccctgaaag tagtactttg
     4801 aggcatagtt atagtcaagt attccaaaga atgacatcaa ggttcttgca ttgtaatcat
     4861 aactaccttc atcaactaat ggtgtagagg ttgtaggata gggtatttgt gggtcaacat
     4921 cgggtgagta tatgtaataa gaccagtaag tacgcctcat agcttcctgc ccaactagaa
     4981 atctaacatt atgatcggca gcaaaaacgt tgttataagt tacggtattt gaagtagtca
     5041 gctgaacata ttggagtgta gatgattgta atgttccggt agttcctcct ccggcttcgg
     5101 gcgaccaata cctacgcccc tcaccaaatg taccttcagc tgcattgtta gtcttgattt
     5161 ccaacttttc gataggcttc caggttaaaa aataggtacc ctgtacgcga tactgtttct
     5221 cccactgatc atcatactca gcagttgccc ggggatttgc attcgaattt tcaggaatat
     5281 taatgttgta agaaccgtca gcattatatg caggtgtcca tggctgaatt gtcatgccag
     5341 caaaagcggg attaacatag tataatttct gcatggcaac gtcattggat tcagtatagg
     5401 caacgttcat acgagcccct gtttccagtg ttttggtaag tttaaaatcg gcatttacac
     5461 gagccgaaaa cttctggaaa tcaattccat agaaaacgcc atcgttctta tggtacgaaa
     5521 gcgatgtgta aaagttgctc ttattggtac caccagaagc attaatttca tactcctgca
     5581 gattccctaa acgcgtgaaa agattcatcc agtttgtttg ttcaccagct aagaggctca
     5641 tggggcggta gaaacttcca ttggggtcat caggatcttt accagcattg attatagcct
     5701 gacgttgaaa gctaagcagc tcctcgccgt tcattaccct gaaatcatta tcattggcta
     5761 gttgagaaat tccaaactta gaacgtgcat aaaatttggc tttcccctct tttccacttt
     5821 tagttgtaac aagtattacc ccattagcag ctctagaacc atagacagaa gcggcagcag
     5881 catctttcaa tacggtaatt gattcaatgt cgttagggtt taaacttgcc atagcatttc
     5941 ctgtattggt gaaataggat tgattacctc ccatcacagg aattccatcg acaacccata
     6001 acggttcact accagcatta attgaagatg ttccacgaat acgaatttga gaatcagccc
     6061 ccggttgtcc ggatgaagca gtaacctgaa ctcctgccag cttaccagca agagccttgt
     6121 caaccgatga aacaggtatc tcagcaatat tctcactact tacagtggtt acagaacctg
     6181 ttttagcctc acgcttaaca gttccataag ccacaacaac aacctcttcc atctcaacgg
     6241 tttctggttt tagggaaata tcaatcaccg atcggccggc aattgcaaca tcctgtgttg
     6301 taaaaccaat aaagctaaat gaaagagtag tagcattagc tggaacgtta atctcgtact
     6361 taccatcatt acttgtgctc accccgatgg tagtaccggg aac
//
LOCUS       DAOSHM010000098         6380 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_10686_length_6380_cov_18.391621, whole genome shotgun
            sequence.
ACCESSION   DAOSHM010000098 DAOSHM010000000
VERSION     DAOSHM010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 6380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6380
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10686_length_6380_cov_18.391621"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(23..1273)
                     /locus_tag="PK839_10035"
     CDS             complement(23..1273)
                     /locus_tag="PK839_10035"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG73113.1"
                     /translation="MERKSSKLEELAMSGSPIQLAIRRLKASWIAVASFCLIGIVVLI
                     SILGYLITPDPTPYANNQHLELSSCKPGAEVTMLKVRRQQDIQHRSWFGRMLYGDESD
                     YREIPINSFYFEDASIVIEGYNPIPESEPVVARFSLAEVAYRIEPPAKPIIRNDSVLF
                     VQADGGATAMTVSQLQERVEKENFVTHTYILGTDRFGRDLLSRMIIGARVSLSVGFIA
                     VAISLLIGILLGSLAGFLRGWVDSAIMWFVNVVWSVPTLLMVIALTMVIGKGFWQIFV
                     AVGLSMWVDMARVVRGQVLSVRENGYVEAATVLGLSRFRVIFRHILPNIMGPVAIVCA
                     SNFATAILLEAGLSFLGIGVQPPMPSWGSMIRDHYGYIIMDKAYLAILPGFAIMMLVL
                     AFNLLGNSLRDALDPQTIRNNSPK"
     gene            complement(1240..2358)
                     /locus_tag="PK839_10040"
     CDS             complement(1240..2358)
                     /locus_tag="PK839_10040"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOG73114.1"
                     /translation="MLTIATIAITLLYVVLIVLLIVGLVQPSAGHNQAFGQLAQPVSV
                     VVAFRNEAPNLTRLLEALSRQTLDASKWELIMVDDGSTDGGDKIAQSFHNLPGLRLVK
                     LQKGITGKKQALLFGVELARFNLVAITDADCVPVATWLNDISRRANGYALLQGCVLVE
                     RNSNLLSWFEALDYASLMAVSAGSFGLKRPVVAASANLVFRKDMINVRPETLRVDVAS
                     GDDMFLLHEVKKDHSNNMLFSTNLAGSVLTRFDGGFVGAIRRRRRWASKAPAYTDIET
                     ILVGLIVLVFNLWLLVLPMICIFNLEAWNLLVGSLLVASLANFCLLFIYLKQTNQLKL
                     MLVFLPLQLIYPIYLCTVAIAGFAGGQNWKGRAVSWKN"
     assembly_gap    2434..2533
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2579..3472)
                     /locus_tag="PK839_10045"
     CDS             complement(2579..3472)
                     /locus_tag="PK839_10045"
                     /inference="COORDINATES: protein motif:HMM:NF024762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HOG73115.1"
                     /translation="MVDKSYIHSLITLLDDPDPEIFNAVSEKIITQGITVVPLLEGAW
                     GSSDSRIFQERLENLIQTIQFNTVANALTQWKDSESNNLLEGAVLVARLQFPDLSLQP
                     IELEIKKLQKDIWLEINSNLTALEKVRVINHVIFTIHKYEPNTSNFFLPGNHLINHVV
                     ETKRGGPIMLAILYAEIAQRLGLPISCVNLPKNFVLAYQDRYRVPAERKGKDPESILF
                     YINPFNNGSVFSRNEIELFLSRQQLEPKPEYFLPCANTTTISQLVFNLIYSLERNGNR
                     SKANEYKKLLQILEKDGEEQG"
     gene            complement(3483..4358)
                     /locus_tag="PK839_10050"
     CDS             complement(3483..4358)
                     /locus_tag="PK839_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HOG73116.1"
                     /translation="MRVIESSELILNQDGSVFHLHLKPEQLADTVILVGDPGRVETVS
                     SYFDRVELTVSNREFKTCTGTYKGKRISVVSTGIGTDNIDIVVNELDALANIDLASRT
                     VKANHTKLTLVRLGTSGALQPEIELGSSVLTEISIGFDGLLNFYANRNGICDLEMEQA
                     FLKHTQWNPLCAKPYFCHSSRKLVELFAGITVKGVTISAPGFYGPQGRVLRLPLADPT
                     LNDKIVDFRYKGYKITNFEMESSAINGLSLLLGHDSVTICTIIANRVVKDAKVDYQLA
                     MKNLIENTLNRLASI"
     gene            5519..6181
                     /locus_tag="PK839_10055"
     CDS             5519..6181
                     /locus_tag="PK839_10055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG73117.1"
                     /translation="MNSITKRCYIALLLILTASPAFCQSGDTVEAKQGVKVELGYMVI
                     SNSLNADDYHDFKYMHVIDNANAFIRGANLKFTFPTRWKYLDATAGALLIRCLDESGT
                     SAIDVSHINADAYNLNGGGVYLGISPKLKGKHFGLTSDLAVGVLSFKEYISIVNNVIA
                     PYVDEHNLKASYGLGAVSSVGFYVSFWRIGINPSLTCIFSGGASSSFTFWGLNIPITI
                     QF"
BASE COUNT         1693 a         1499 c         1457 g         1631 t
ORIGIN      
        1 tttatccttt atccttatta tttcatttgg ggctattgtt cctgatggtc tggggatcaa
       61 gggcatccct aaggctgttg cccaacaggt taaacgccag gaccagcatc attatggcaa
      121 aaccgggcag tatagccagg tatgctttat ccatgatgat gtagccgtag tgatcgcgaa
      181 tcattgagcc ccatgagggc atgggaggtt gaacaccaat tcccaaaaag ctaaggcctg
      241 cctccagcaa aattgcagta gcaaagttgc tagcacatac aattgccact ggccccataa
      301 tgtttgggag tatgtgcctg aaaattaccc taaacctgct taatcccaga acggttgctg
      361 cctcaacata gccattttct ctgaccgaca aaacctgccc cctcacaaca cgagccatat
      421 caacccacat gctaaggcct actgccacaa atatttgcca gaaaccctta ccaataacca
      481 ttgtcagggc gattaccatg agtaaagtag gaactgacca aaccacgtta acaaaccaca
      541 tgatggcgct atcaacccaa cccctcagaa atccggccag cgaacccagt agtattccaa
      601 taagcaggga tatggcaacg gcaataaatc caacggacag ggaaactctt gcaccaataa
      661 tcatcctgct cagcaggtcg cggccaaacc gatcggtgcc cagaatatag gtgtgggtaa
      721 caaaattctc cttctccacc ctttcctgca gctggcttac cgtcatagct gttgctcctc
      781 catcagcttg aacaaaaagg acagaatcgt tacggattat cggcttagca ggtggttcaa
      841 ttctatacgc cacctctgca agagaaaatc tggctacaac aggttccgat tccggaatgg
      901 gattataccc ctcaatcaca atgctggcat cttcaaagta aaagctatta atgggaattt
      961 ccctataatc ggactcatcg ccatagagca ttctcccaaa ccatgagcgg tgctgaatat
     1021 cctgttgtct tctaacttta agcatggtaa cctcagctcc tggtttgcag gatgacagct
     1081 ccaaatgctg gttgttggcg tagggtgttg gatcgggagt aatcaggtat cctaatatgg
     1141 aaatcagcac aacaatcccg atcaggcaaa atgaggcaac agcaatccag ctggctttca
     1201 accgtcggat ggctagctgg atgggtgagc cactcatggc tagttcttcc agcttactgc
     1261 tcttcctttc caattttgtc ctccggcaaa accagctatt gcgaccgtgc ataggtaaat
     1321 ggggtaaatc agctgtaaag gtaaaaaaac aagcatcagc ttcagctggt ttgtttgttt
     1381 caggtatatg aagagtaggc aaaagttagc cagtgaggca accaacaagc tgccaaccag
     1441 taggttccat gcctctagat taaagatgca aatcatcggt agaactagca accaaaggtt
     1501 aaaaaccaaa actatcagcc ccacaagaat ggtttcgata tcggtgtagg caggggcttt
     1561 ggatgcccaa cggcgacgac gtcgaattgc cccaacaaaa cctccgtcaa aacgggttag
     1621 tacgcttccg gcgagattag tgctgaatag catgttgttc gagtggtcct tcttaacctc
     1681 gtggagcagg aacatatcat cacccgatgc aacatcaaca cggagcgttt caggacgcac
     1741 gttaatcata tccttacgaa aaacgaggtt agcggaagct gcaactacag ggcgctttag
     1801 cccgaacgac cccgccgaaa cagccattag cgaggcgtaa tccaacgcct caaaccagga
     1861 gaggaggttt gaattccttt caactaaaac gcaaccctgt agcaaggcat acccattggc
     1921 ccttcgggaa atatcgttaa gccatgtagc aacaggaaca caatcggcat cagtgatagc
     1981 taccaggtta aacctagcca attcaacccc aaataacaag gcctgctttt tcccggtaat
     2041 gcccttctga agtttcacaa gccgtaaccc cggcaggtta tgaaatgatt gtgcaatttt
     2101 atcaccccca tctgttgacc catcatcaac catgattagc tcccacttgc ttgcatcaag
     2161 ggtttgacgg cttaacgcct caagcaatcg ggtaaggttt ggagcctcgt tccgaaacgc
     2221 cacaaccacg ctaaccggtt gagccagctg gccaaatgct tggttgtggc cagctgacgg
     2281 ttggactaac ccaacaatca gcagcacaat caggacaacg tagagcaggg tgatggcaat
     2341 tgttgcgata gtaagcatga agcaaaatgc cttgtactgg gttaaaatta gtgcaaataa
     2401 aatgagaaga agctaaatgc gtaaaaaata tgannnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnagccgga caagttccgc cagcaggcgg acaagtttat cttatccttt
     2581 acccctgttc ttccccatcc ttttcaagga tctgaagcaa ttttttgtat tcgttggcct
     2641 tgctgcgatt accgtttctc tccagagagt aaatcaggtt gaataccagc tgtgaaattg
     2701 ttgttgtgtt ggcacatggc agaaagtatt cgggtttagg ctccagctgc tgtcggctaa
     2761 ggaatagctc tatctcgtta cggctgaaaa ccgaaccgtt gttgaatggg ttgatgtaga
     2821 acaggatact ttcaggatcc ttaccttttc tttcagcggg cacgcggtac ctgtcctgat
     2881 aggcaagcac aaagttttta ggaaggttta cgcacgaaat gggcaaccct aaacgctggg
     2941 caatctcagc gtaaagaatg gcgagcataa taggaccacc cctcttggtt tctactacat
     3001 ggttgataag gtggttgccg gggaggaaga agttggatgt attaggttca tacttatgaa
     3061 tggtaaagat tacatggtta atcaccctga ccttttccag tgcggtgagg ttgctgttta
     3121 tttcgagcca gatatccttt tgaagcttct taatctccaa ttcaatgggc tggagcgata
     3181 aatcgggaaa ctgcagacgg gctaccagca ctgctccctc tagtagattg ttactttccg
     3241 aatctttcca ctgggtgaga gcgttggcaa ccgtgttaaa ctgaatggtc tgaatcagat
     3301 tctccagcct ttcctgaaat atcctgctgt ccgacgaccc ccatgcaccc tcaagcagcg
     3361 gaacaacggt aatgccctgc gtgatgattt tttccgacac ggcgttgaaa atctcggggt
     3421 cgggatcgtc aagcagggta atgagggagt gtatgtagct tttgtccacc acttgcagtg
     3481 tgttaaatgg atgcaagcct gttcagcgtg ttctctatca ggtttttcat ggccagttgg
     3541 tagtcaacct tggcatcctt tactacccgg ttggcaatga tggtgcagat ggtaacggaa
     3601 tcatggccaa ggagcagcga aagcccgttg attgccgagc tttccatttc aaagttggta
     3661 atcttatagc ccttatacct gaaatctaca attttatcgt tgagcgttgg atcggccaac
     3721 gggagtcgca gtactcgtcc ctgcgggccg tagaaaccag gcgccgaaat ggtaactcct
     3781 ttcacggtta taccggcaaa tagctcaacc agcttgcggg acgaatggca gaagtatggt
     3841 ttggcacaaa gcggattcca ctgggtatgc ttcaggaagg cctgctccat ttccaggtcg
     3901 catattccat tccggttggc atagaagttc aacagtccat cgaaaccaat gcttatctcg
     3961 gtaagcaccg agctgccgag ctctatctcg ggttgaagcg cccccgatgt tcccagcctc
     4021 accagggtga gcttggtgtg gttggccttg actgttcggc tggccaggtc aatattggcc
     4081 agggcatcca gctcgttcac cactatgtcg atgttgtcgg ttcctattcc tgttgaaacc
     4141 acgcttatcc gcttgccttt gtatgttccg gtgcaggttt tgaactcccg gttcgatacg
     4201 gtgagctcaa cccggtcaaa gtacgaggaa accgtttcaa ccctacccgg atcaccaacc
     4261 agaattacag tatctgccag ctgttcgggt ttaaggtgta ggtggaagac gctgccatcc
     4321 tggttcagta tcaactccga cgactcaatt actctcatag ctactagttt tgctgccaaa
     4381 aatactaaaa atcgttcatt ttatggtcta atagctacaa agacttaacg ttttttttac
     4441 atgtcatcca ttgcataaaa aattgaacta ttcctggcat gcacattcca ttttgtatca
     4501 aaaaatgcca gtatctatgt tatcaacacc taaatcctta ggacggttag cagcgtgttg
     4561 atagcctgtt tgcagcttgt ttataattct aatgtccttg tggtggtgcg gggttcattg
     4621 ggcaattcat tccccccgct actgcaaaag tcccctcgtg tggtggtata gagaaagaaa
     4681 gtagtgggca ggaattagca aaagaataat ccggctggac tatacggtat atacttaaag
     4741 atagacgtgg taagcgttgt tggcaggcag ggatgggttt gccacacgac aggagttgtg
     4801 cggcagcgat gccgaagcgg gtaaagctgg taaaagctgc atatcccttg taaattcaac
     4861 catacacaaa gccccgggta acccaagctc tactttaatg cccgaccaag ctcaacactg
     4921 tcctgcagca agcccctagc ttcgcccgaa tttccgactc ccgacttgtt gcaaatcgca
     4981 ttggaacgct gcaggtgcca ctgggaatag aaaagccgtt atcatcatta ttaacacgca
     5041 gacctctata gcggttagca gcgtgttgat agcctgtttg taacatgttg atagcttatt
     5101 cttcaggatg gcggagcagg agtaatggcc gttatcagca gccaaaattt cccccagcct
     5161 ccacccgggc tctttagcaa tagaaaaaaa cttgacacga gctggaatat gccgtacaat
     5221 tgcataaaaa atttgccggc aggttaggcc ggttaaaaat tataggaaat gagcttgagc
     5281 tgactaaaaa agtaatcaac ctttgggcta cgggagatgg cctgtcggtt gcggaaaaag
     5341 ggtaaggcgc ttcccctcat atatcaaaat attttctaat gaatgccttt attagtttat
     5401 ttcattgtga agaacagaaa aaaactcgtt acattttttt tcttccttca gtgtctatcg
     5461 ctaggttttt cgaatagttt caagctacta atgagtataa gccccaaaaa atgaatttat
     5521 gaatagcatc acgaaacgtt gctacatagc actgctgctc attctaacgg cttctccggc
     5581 gttttgccag agtggcgata ccgtggaggc gaagcagggg gtgaaggtag agctgggcta
     5641 catggttatt tccaatagcc ttaacgctga cgattaccat gatttcaaat acatgcatgt
     5701 tattgataat gcaaatgctt ttattagggg agcaaattta aaatttacct ttccaaccag
     5761 gtggaagtac ctggatgcta cggccggggc tctcctcatt agatgtttag atgaaagtgg
     5821 cactagtgca attgatgtaa gccatatcaa tgctgatgca tataatctta acgggggcgg
     5881 tgtttacctg ggcatcagcc ccaagctgaa ggggaagcac tttgggttaa cctccgacct
     5941 tgcagtgggg gtgctgtcgt ttaaggagta tattagcatt gtaaataatg ttatcgctcc
     6001 ctatgtggac gaacacaacc taaaggcctc ctacggtctg ggtgcggtta gctcggttgg
     6061 cttctacgtc agcttctgga gaataggcat caacccatcc ctaacctgta ttttctcggg
     6121 tggagccagc tccagcttta ccttctgggg cttgaacatt cccattacca tccaattcta
     6181 aaaatacgtc tgccttatgt cgtggagtag ccaggctgca aagtccggct actcttttta
     6241 cccatcgggt tgaagtaatc tgaacaagtg gagcagcaca caacagcagt tcgttatcaa
     6301 acgtccgcca taaaccgtca tcagccggac aagttcttat atcttgaatt ttatatccgt
     6361 caggagccgg acaagttccg
//
LOCUS       DAOSHM010000099         6122 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_11311_length_6122_cov_22.306082, whole genome shotgun
            sequence.
ACCESSION   DAOSHM010000099 DAOSHM010000000
VERSION     DAOSHM010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 6122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6122
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11311_length_6122_cov_22.306082"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1043)
                     /gene="queA"
                     /locus_tag="PK839_10060"
     CDS             complement(<1..1043)
                     /gene="queA"
                     /locus_tag="PK839_10060"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HOG73118.1"
                     /translation="MKLSQYKYNLPQDLIAKFPTENRDESRLMVLNRKNHKIEHRIFK
                     DVIDYIDENDVVVFNNTKVFPARLYGNKEKTGAEIEVFLLRELNREQRLWDVLVDPAR
                     KIRIGNKLYFGDDDILVAEVIDNTTSRGRTLRFLFDGPYEKFKETLYKLGETPLPKFI
                     RREVVPEDRERYQTVYAKHEGAVAAPTAGLHFSKQLLKRMEIKGINFAEITLHVGLGN
                     FRSVDVEDLTKHKMDSEQINIPEEAAEIINRAKQNHHRVCAVGTTVLRTLESSVTTSG
                     QLKPFSGWTNKFIFPPYEFSIPDMLITNFHLPQSTLMMMVSAFSGYDFLMEAYKLAIK
                     EKYRFGTYGDAML"
     gene            complement(1174..1887)
                     /gene="truB"
                     /locus_tag="PK839_10065"
     CDS             complement(1174..1887)
                     /gene="truB"
                     /locus_tag="PK839_10065"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HOG73119.1"
                     /translation="MDVNNFNATKEGTAILVDKPLGWTSFNVVGKIRTLIHRATGDRT
                     IKVGHAGTLDPLATGLLVICTGRATKQIDSLMNGKKEYLATLKLGETTPSFDLETPPD
                     GQFPTEHITPTLIAETLKNFMGIQHQTPPLFSAKYINGKRAYKYARKGIEVALEPVPI
                     EIFEMEMLSFEPPLLTLRICCSKGTYIRALARDIGVALGSGAHLVALRRTVSGDFRIS
                     EALSIQELEKIFSSPMQQT"
     gene            complement(2215..2484)
                     /locus_tag="PK839_10070"
     CDS             complement(2215..2484)
                     /locus_tag="PK839_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3098 domain-containing protein"
                     /protein_id="HOG73120.1"
                     /translation="MAKNANKPADSSQVEGKFALGKENYKLLLIGFGIIILGFILMAG
                     GGSKDPNVFDPRIFSFRRITLAPIVVLGGFVFEIYAIMKKPKHKS"
     gene            complement(2521..3399)
                     /locus_tag="PK839_10075"
     CDS             complement(2521..3399)
                     /locus_tag="PK839_10075"
                     /inference="COORDINATES: protein motif:HMM:NF037183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HOG73121.1"
                     /translation="MKNQTKKSAKGRLRNSYISSIISISLVLFTLGILGLLVISSQKL
                     SDYMKENIGFTIYLNDSVSNADANYLKKLLDASTYVRSSQYYSKEDAASLMEKELGED
                     FISYLGYNPLSAFIDVRLKADYANNDSIPKIESWLKQFQQVKEVDYQKSLVNLVNENV
                     SRLGLVVLAFGALMLFIALVLINNTIRLSVYSRRFLINTMKQVGATWGFIRKPFLLRS
                     ILHGLYASIIAIGLLSGLIYGIRNEVADILQIVEPYYIMGIFAAVTLVGIAINLTATL
                     LAVNKFLRLNADDLYY"
     gene            complement(3417..5831)
                     /locus_tag="PK839_10080"
     CDS             complement(3417..5831)
                     /locus_tag="PK839_10080"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HOG73122.1"
                     /translation="MNKILSKALPHVIAVLVFMAISLAYFYPQIQGKRLYQHDIAQWR
                     GSAKEIMDFKEKTGKETLWTNSMFGGMPAYLISANYSGNLMLKVNKAMNFLQRPASFI
                     FIAMLGFYILLLVFGLNPWLSLVGALGYGLSTYFFIIIGAGHNSKIQAIGYVAPMIAG
                     LVMACKGRFWGGMAIFGFFFGLNLASGHPQITYYSAFIMLALLVAFFINAQKTKTLKT
                     IYKAIGFLAIAGILAVGSNFSNLWFTYDYGKDSIRGKSELTDNQHNKTSGLDKDYVTQ
                     WSYGVAETFNMLIPNLMGGASSMDVGENSETFKFLRQNGVPFSQAQTIVKQLPTYWGP
                     QPSTSGPVYVGAIIFFLFILGMFLLRGPEKWALFAVTLLAILLAWGHHFNFLTNFFLN
                     YFPAYNKFRTVSMILVVAELTMPLIGFLGLKEIYEGKIAKPDFLRAFKWATGITAGIC
                     LFFLIFGRGIFNFSGAVDQQLGWPKELIDVLKRDRQHMLTNDSLRSLFFVLAGAGIVY
                     AFFIKKIKPSYFLLSLGLLITIDMWVVNKRYMNNDGFVNKRMVEAPFTPTDADKQILA
                     DKSPDFRVFNLTVSPFNDASTSFYHKSIGGYHGAKLRRYQDLIDYHLSKGNMAVLNML
                     NTKYFIQPGQNGPIALPNPDALGNVWFVDSIKLVNNPDEEIQALYNFNPKTTAIVDKK
                     FESLLADFKPDNSGAGNIELVEYAPNMLKYHSISNQPKLAVFSEIYYPKGWKASIDGK
                     PAPHFRANYVLRAMIVPAGEHRIEFTFDPPMWRVGKSIDLTSSLLLILIFVGWIGWSA
                     FSKKSI"
BASE COUNT         1627 a         1335 c         1442 g         1718 t
ORIGIN      
        1 atgagcatgg catcgccata ggttccaaag cggtacttct ccttaatggc cagcttgtag
       61 gcctccatca ggaagtcgta gcccgagaat gcagagacca tcatcatgag ggttgactgg
      121 ggcaggtgga agttggtgat gagcatgtcg gggatgctga actcgtaggg agggaaaatg
      181 aacttgttgg tccatccgct aaatggtttc agctgtccgc tggtggtaac ggaactttca
      241 agcgtgcgta gtactgtagt tccaaccgcg caaaccctgt ggtggttttg ctttgccctg
      301 tttataatct cggcggcttc ctcgggaatg ttgatctgtt cggagtccat tttgtgcttg
      361 gtgaggtctt ccacatcaac cgagcggaag ttgcccagcc ctacatgaag ggtgatttcg
      421 gcgaaattaa ttccctttat ctccatacgc ttgagcagct gcttgctgaa gtgcaggcct
      481 gcagtaggag cagctactgc tccctcatgc ttggcgtaaa cagtttgata gcgttccctg
      541 tcttcgggga caacctccct tcttataaac ttgggtaaag gtgtttcgcc tagcttatag
      601 agagtttcct tgaacttttc gtaggggccg tcgaagagga accgcagcgt tctccccctg
      661 ctggtggtgt tgtcaataac ctcagccacc aaaatatcat catcaccgaa gtacagctta
      721 tttccaatac ggatttttcg ggcaggatca accagtacat cccatagccg ctgctcgcgg
      781 ttcaactccc gcaggaggaa tacctcaatc tccgctccgg ttttttcctt attgccataa
      841 agccgtgccg ggaaaacctt ggtattgttg aacaccacca cgtcgttctc gtcaatgtaa
      901 tcaatcacat ccttaaaaat gcggtgctca attttatgat ttttccggtt aagcaccatt
      961 aaacgtgact cgtcgcgatt ttcggtagga aacttggcga tcaaatcctg tggcaggttg
     1021 tacttgtact gtgatagctt catattgttg gacggttaat tcttaggacg tttttccccg
     1081 aatctcatgc cttgagcagc tgctaaagct taaactttta ttgtagggga aaataattat
     1141 gcaacaaaca gtcccacgtt atacgtgcca tttttaagtt tgttgcatgg ggcttgaaaa
     1201 tattttttcc agctcctgaa tggacagggc ttcggatatt ctaaaatcac cgcttacagt
     1261 tcgccgtagg gccacaagat gcgccccgga gccaagcgca acaccaatgt cacgggccag
     1321 tgctctaata tatgttccct tgctacagca tattcttagg gttagcaatg ggggctcaaa
     1381 gcttaacatc tccatttcaa atatttcgat gggtacgggt tccagggcta cttcaattcc
     1441 cttacgggcg tacttatacg ctctcttccc atttatgtac tttgctgaaa acagaggagg
     1501 agtctggtgc tgaattccca taaagttctt tagggtttct gcaatcaggg ttggagtaat
     1561 atgttctgtc ggaaattgcc catcgggggg tgtttccaag tcaaaagaag gggtggtttc
     1621 tcccaatttt agcgttgcca agtactcctt tttcccgttc atcaggctgt caatttgctt
     1681 ggttgcccta cctgtgcaaa taaccagtag gccggtggcc aagggatcaa gggttccagc
     1741 atggcctacc ttgatggttc tatcaccggt agcccgatgg atgagcgtcc taattttacc
     1801 taccacgtta aatgacgtcc atccaagagg cttatcaacc agaatggctg ttccctcctt
     1861 ggttgcgtta aagttattta cgtccataaa ttgaaaatgc tggtggccaa taattgtaac
     1921 gatttatgct gtatcatttg aaggagagca aagaagagtc tattctaatt ggggaaagca
     1981 tttaatattt tgcatcatgc gatgttggcg gtgtaaatgg ccacaaaaaa gtagttttgc
     2041 aagatgaatt acccaagcag ccttaacgga cataagccgg caagaaactt gatgcgtacc
     2101 tttaccgtgg agttgtccag cgcaatacta cgcgtataaa gatgttagca ttgccatctt
     2161 tacaataccc aatagcgtaa aattggatag ggtggctatt cctgttgtcg gcgactaact
     2221 cttgtgtttt ggttttttca taatagcata aatttcaaac acaaagccgc ccagcacaac
     2281 aataggggct agtgttatac gcctgaagct aaagattcgc gggtcaaaaa cattggggtc
     2341 ctttgaaccg ccaccggcca tgagaataaa tcccagaatg atgatgccga atcctatcag
     2401 caacaacttg taattttcct ttccaagcgc gaacttcccc tcaacctgag agctatcggc
     2461 cggcttgttt gcatttttgg ccatatcagc gtagtattaa atagtttgaa atgttagctg
     2521 ttagtagtat aggtcatcgg cgtttagcct caggaacttg tttactgcta agagtgtagc
     2581 tgttaagttg atagctattc ccaccaatgt aaccgctgca aatataccca taatatagta
     2641 gggttcaaca atctgaagaa tatctgcaac ttcgtttctt attccgtaaa taagtccgga
     2701 aagcaggccg attgcaataa tagaggcata gaggccatgc agaatgcttc ggagaaggaa
     2761 tggcttacgg ataaaacccc aggtggcccc cacctgcttc atagtattta tgaggaacct
     2821 acgggagtaa accgaaagcc ggatggtgtt gttaataagc accagtgcga tgaaaagcat
     2881 cagggcgcca aaagccagca ctaccaaacc caaccggctt acattttcgt tgaccaggtt
     2941 gaccagcgat ttttggtagt caacctcctt gacttgctgg aactgcttca gccagctttc
     3001 aatttttgga atgctgtcgt tgttggcata gtcagccttc agcctgacgt cgataaaggc
     3061 agaaagcggg ttgtatccaa gataggaaat gaagtcctct cccagctcct tttccataag
     3121 gctggctgca tcttccttgc tgtagtactg cgaagatctg acgtaggttg aggcatcaag
     3181 aagttttttc aagtagtttg catcggcgtt actaacgctg tcgttaaggt aaatggtaaa
     3241 gccaatattc tccttcatgt agtcagagag tttttgggag ctaataacga gcaagcccag
     3301 tatgcccaag gtgaaaagaa ccagagaaat gctaatgata gacgagatgt aggaatttcg
     3361 caaccgcccc ttagccgatt ttttggtttg gttcttcatg ggtaggatgg cttcaattag
     3421 atagatttct tactaaatgc gctccaccct atccagccta cgaatattaa aatcaggagc
     3481 agggaggagg tcaggtcaat actttttcca accctccaca ttggggggtc gaaggtgaac
     3541 tcgatacggt gttccccagc cggtactatc attgcccgga gaacatagtt tgccctgaag
     3601 tgcggcgcag gttttccatc aatgctggcc ttccaccctt taggatagta gatttcagag
     3661 aaaactgcca attttggctg gttggatatt gagtggtatt tcagcatatt tggtgcgtat
     3721 tcaaccagct caatgtttcc tgcaccggag ttgtcgggtt taaaatcggc tagcagcgat
     3781 tcaaactttt tatcaacaat agcggtagtt ttagggttaa agttgtagag tgcctgaatt
     3841 tcctcgtcgg ggttgttaac aagtttgata gaatcgacaa accaaacatt tcctagtgca
     3901 tcggggttgg gtagggctat aggaccattc tgtccgggct gaatgaagta cttggtattc
     3961 agcatgttca aaacagccat gttacccttt gaaaggtggt agtcaataag gtcctggtag
     4021 cggcggagct tagctccatg gtagccgcct atagacttgt ggtagaagga tgtagaggca
     4081 tcgttgaaag ggcttaccgt aaggttgaac accctgaagt ccgggctttt gtcggctaaa
     4141 atctgcttat cggcatccgt gggggtaaaa ggggcttcta ccattcgttt attaacaaag
     4201 ccatcgttgt tcatgtagcg cttgttaacc acccacatat ctattgtaat gagcaggcct
     4261 aaggaaagca ggaagtagga aggttttatt tttttgataa aaaatgcgta aacaatgccc
     4321 gctccggcaa gaacaaaaaa tagcgaacgg agggagtcgt tagtcagcat gtgctgccgg
     4381 tctcttttta ggacatcaat aagttccttt ggccagccaa gctgctgatc aacagctccg
     4441 ctaaagttaa aaatgcccct tccaaatatc aagaaaaaca agcaaattcc tgcggtaata
     4501 ccagttgccc atttaaatgc cctgagaaag tctggtttag caatttttcc ctcataaatc
     4561 tcctttaagc ccagaaaacc tattaggggc atggttagct cggcaaccac cagaatcatc
     4621 gagactgtcc ggaatttgtt gtaggccgga aagtagttga gaaaaaaatt tgtaaggaag
     4681 ttgaagtggt gcccccaggc tagtagaatt gccagcaggg taactgcaaa caatgcccac
     4741 ttttcggggc ctctaagaag aaacatgcct agtatgaaga ggaaaaaaat gatggcgccc
     4801 acgtaaacag gtcccgaggt tgagggttgt ggtccccagt aggttggcag ctgcttcaca
     4861 attgtttgcg cctggctaaa gggaacccca ttttgacgca ggaacttgaa tgtttcggag
     4921 ttttcgccaa catccatgga gctggcgcct cccataaggt tggggattag catgttgaag
     4981 gtttccgcaa ctccgtagct ccactgggta acgtaatcct tgtcgagtcc cgaggttttg
     5041 ttgtgctggt tgtcggttag ctcagatttc ccccttatgg agtctttgcc gtaatcgtag
     5101 gtaaaccata ggttgctaaa gtttgatccc acagcaagaa ttcctgcaat tgcaagaaat
     5161 ccgatggcct tatagatagt tttcagggtt ttggtctttt gggcatttat gaagaacgca
     5221 acaagcaggg ccagcatgat aaatgcgctg tagtaggtaa tttgtggatg cccacttgcc
     5281 aggttaagcc caaagaaaaa gccaaatatt gccattccgc cccagaacct tcccttacag
     5341 gccataacca gtcctgcaat catgggtgca acatagccaa ttgcctgaat tttggagtta
     5401 tgccctgctc caattatgat gaaaaaatat gttgataggc cgtagcctag cgcaccaacc
     5461 aaactcagcc aaggatttaa tccgaaaaca agcagcagga tgtagaaacc aagcatagca
     5521 ataaaaatga agctggcagg acgttgtaga aaattcattg ccttgttaac cttcagcatc
     5581 aggttgcctg aatagttggc tgaaatcagg taggcgggca ttcctccaaa catgctgttg
     5641 gtccataggg tttccttccc cgttttttcc ttgaagtcca taatttcctt agccgatccc
     5701 ctccactggg caatatcgtg ctggtagagc cttttgccct gaatttgagg ataaaaatag
     5761 gcaagtgaaa ttgccataaa cactagcacg gcaataacat ggggtagggc tttgctcagg
     5821 atcttattca tctgcttaag tttatgttac tgacactaca gctggttgta aaagtaaatg
     5881 aacctagaaa acttatgttt ctacttgctt accataccga gctatattcc ctatgaatta
     5941 aaatgaattt tgaagcagtt caaaatttac ccatgggtag ctcaacagtt tagcgttcaa
     6001 atatacgcaa aaattacggt gatgggttaa aatcatattt gaaatggctg aacagtagtt
     6061 cttttaggta tgatgcttta ctaaaaattt tttcaatgtg ggctatttgg atttcctgaa
     6121 aa
//
LOCUS       DAOSHM010000100         6109 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_11347_length_6109_cov_3.700033, whole genome shotgun sequence.
ACCESSION   DAOSHM010000100 DAOSHM010000000
VERSION     DAOSHM010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 6109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6109
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11347_length_6109_cov_3.700033"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>6109)
                     /locus_tag="PK839_10085"
     CDS             complement(<1..>6109)
                     /locus_tag="PK839_10085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG73123.1"
                     /translation="YILFSGGGTVNVQGTISGGGITSTAGGGTGAPTAGTVNYNNSGN
                     QNVGAYTYYNLTISGSGNKSLTGNTTVNSTLTLNGGVLQLGGNNLTLATAASSNILGG
                     PFSSTCMVETNGTGYLQQRVPATPYTVPIGSNGTYAPVTVQSISGSTYLRFRTVYSTS
                     LGSQYLKRYWQLTGSAATTATITFGYDPTENPKDPTKIWYRNGGAWSQPTGTQSFDGI
                     NRKFTITGTTNISAATTEWTAGYPPKTFFSHQSGSWNDASTWTSDPGGTTYENIGTPT
                     DSSVVVILPDRTVSLASNVSNVQLEVNINEGGILDMATYSFSSGLKELDGGGTLKLAS
                     VNFPTATTNTFVNAGGGTTEYYNSANFTLPAAQTTYNHLRINAPGFTATQLSNITLNG
                     NLHVKQGTYRINDNSANRRQLIIHGDVTVDAGASITVGTGVTNTITDPTTAAESGTAP
                     YITYYDAHSHRVVIYGNLTNNGTVRFTNLSYPVYNAFPPTTLGPTTGFATVYFVGASS
                     NDLYCNGTTDFYNLVLDKGVDQTYSLTVYSTAYANFRLFGANNAGGYGGGANPNLCKA
                     LWIRNGTMVLQGSTIIPSLSEGNCDQGTGGPNSDFYVPANGALVLDGDNVVVLATADD
                     YREVNVAYGVSAPDNATMGVNAGAGCSSFSTLGLLRVNKGYISTRESGGFIQWSQSSG
                     QFEINGGRVDAKQYRTAGTAGGLASYTQTGGLFELRGRFWRTPSAYTSVDDLVNAPIS
                     TNRSSGGLQALVGTFNLNETTNVFNMSGGTIRIFDVCGDGTAANQQKAVEILSSKNNI
                     NVTGGTLEVMPTTGTGLADSPIFSITSNAEFGNFTVNRQSSVAVVTMVTGYPLTVLAN
                     YSLLSGEFNANSQNVTIGGNMFVANGTTYTSGNNWTTFNGSANQTITANTASALVFKK
                     FRMDKPAGKILNLAGAQTDFQVADSLMLVSGTLNDGGKTLSLVTSATSSTSYVFNSAL
                     HLGTGKIAIADDDPTVVDGSGSGIFKNLELNNSDALAAPVSLRANTTISGVLTFARDK
                     LLDISTYNLKLTSTASISGYSSSRYIKTAGNAGDGGITRTYSAGSATFVYPVGINGYT
                     PSTLTVNGTPTAWGDITVYPVEGEHPATTVKSRSLTYFWRVKSSGFTLGSATLTHRYD
                     YLQADVVAVGNVTEDGYVAARFNTATSTWTSGAANDVDETNNIIGEPGSGSFLEGVAF
                     IDGDYTAGDNNPTNPFGTPTTYYSRQTGLWGDVNSWSTTGHSGSPAGTVPGAGDIVII
                     GNGNTITSDQNRSCAILKIETGSTLDIYTYTGGNFGMVLNHPNGNGLFRVTTPKAPSN
                     AVPKFFTFPTGDFTEFNANDGTTEYYDIDGTVGALYILPSNVTSYGNLVLRAKGGDNL
                     VLPNNSSTTIHGNLTCTGDATTAWVAMCWNTNIWPYWTNDYNPTLEKTVYVHGNLNVD
                     AGTLIFFNDQQPQHLIVDGDVTVNNGAVLRIYSAYPFSTPVITNNTIKIGGSLINNGT
                     VSLREQSGGNYYYVNSTFFNTGKTYIKNTSGTPSTIFNNLTIDKGLSQVDSLVVSIGG
                     TLTTLTDSWLTLKNGTLVYRRSNPATDFTISAATPFTIPETAGLYIDYPNNSNNRNIL
                     IANAANSSSDLMLDGKLTVKAGNVYIGPTNGTTNNNNDIVYSGDGSSTLDIKGGNLRV
                     NGQIRRSTATTTGAIKYYQSGGNVVINGQGADATALTRAKLEVVNTGSVFSMSGGTIS
                     IVRGGGTSFGDLYLRPASGSVTGGEIIFTQVPSGWAVADAAQAYRLDASIPLNSLKVT
                     GKTTATARSATVTLMVNPLALNGSLTLSNNQSYFDANSGNNLNVSINGDLTNNGTYYP
                     RLNTTTFKGGTQSILGTSTTNFNNLVVNPVTSLTLSSSSINVDGNLTLLSGQLILGNY
                     TVNLKGNFTNNANYTDNGTGVRLIGATQQNISGNGTFSTLELNNSSGARLLSSITLNK
                     DLKLTNGILNINQYKLTLGVNSNITGTSFSATKMIEVDGAFSNVGILKYFPIYDSGPD
                     VTFTYPLGVSNKYTPAVFT"
BASE COUNT         1268 a         1450 c         1792 g         1599 t
ORIGIN      
        1 taggtgaata ctgcgggtgt gtacttgttg ctcactccaa gcgggtaggt aaaggttaca
       61 tccggcccgc tgtcatatat tgggaagtat ttaagaatgc ccacgttgct gaacgctccg
      121 tcaacctcaa tcatcttggt agcgctaaac gacgttccgg tgatgttgct gtttacgcca
      181 agggttagct tgtactggtt gatgttaagt atcccattgg tgagcttcag gtctttattc
      241 agggtgatgg agctgagtag ccttgctccg ctggagttgt taagctccag agtgctgaat
      301 gttccgtttc cgctgatgtt ctgctgtgtg gcgccaatca acctaacgcc ggtgccgttg
      361 tcggtgtagt tggcgttgtt ggtgaagttc cccttcaggt ttacggtgta gttgccgagt
      421 attagctggc ctgacaatag ggtaaggttt ccgtcaacgt taatgctact gctcgacagg
      481 gtaagcgagg taaccgggtt gacaacaagg ttgttgaagt tggtagtgga ggttccaagt
      541 atgctctgtg ttcccccttt aaaagtggta gtgtttaaac ggggataata ggtaccgttg
      601 ttggttaggt ctccatttat ggacacgttg aggttgttgc cggagttggc atcaaagtag
      661 ctttggttgt tcgaaagggt aagcgaaccg ttgagcgcca gcgggttaac cataagggtt
      721 acggttgcgc ttcgggcagt agcagttgtt ttacctgtta ccttaaggct gttcaaggga
      781 atagaggcat ccagcctgta ggcctgcgct gcatctgcca ctgcccaacc agaaggaacc
      841 tgtgtaaaga taatctcccc gccggttacg ctaccggagg cgggtcgcag gtagaggtcg
      901 ccaaatgatg tacctccgcc ccttacaatg cttattgttc cgccgctcat gctgaaaacg
      961 cttccggtgt tcaccacctc cagcttggca cgggtgaggg cggttgcatc ggcgccctgc
     1021 ccgttgatca ccacgttgcc accgctctgg tagtacttta tcgcaccggt ggtggtggca
     1081 gtgctacggc gtatctgccc gttcacccgc aaatttcctc ccttaatgtc gagggtggac
     1141 gatccatcac cggagtaaac aatatcgttg ttgttgttgg tagtgccgtt ggttggccca
     1201 atgtaaacgt tacccgcctt gacggtgagc ttaccgtcga gcatgaggtc gcttgagctg
     1261 ttggccgcgt tggcaatgag tatgttacgg ttgttggagt tgttggggta gtcaatgtaa
     1321 agcccggctg tttcgggtat ggtgaatggg gtagcggcag aaatggtgaa atcggtagcg
     1381 gggttgctcc tgcggtaaac cagcgttccg tttttaaggg taagccagct gtcggtaagt
     1441 gtggtaaggg taccgccaat gctaactacg agcgaatcga cctgggataa acccttgtca
     1501 atagttagat tgttgaaaat agttgatggc gttccactag tattctttat gtaggtttta
     1561 cctgtattaa aaaaggttga atttacatag taataattgc caccactttg ctcccttaac
     1621 gaaactgtgc cattgtttat cagtgaaccg ccaattttaa ttgtattgtt agtaataaca
     1681 ggtgttgaga agggataggc agaatatatc cttaaaaccg caccattgtt tacagtaaca
     1741 tcaccatcta caataagatg ttggggttgt tggtcgttga aaaatattaa tgttccggca
     1801 tcaacgttga ggtttccatg tacatataca gttttttcca gagtagggtt atagtcattt
     1861 gtccaatatg gccatatatt agtattccag cacatggcaa cccatgcagt tgtggcatct
     1921 cctgtacagg ttaaattacc atgaattgtt gtagatgagt tattgggtaa caccaaatta
     1981 tcgccaccct ttgcccgtaa tacaaggttt ccatatgaag ttacattaga aggcaaaata
     2041 taaagagctc caacagtacc gtcaatatca tagtattctg ttgttccatc gttagcatta
     2101 aattcggtga agtcgccggt tggaaatgtg aaaaatttag gaacggcatt cgatggtgct
     2161 ttaggcgttg ttactctaaa tagcccattt ccattgggat ggtttaacac cattccaaaa
     2221 tttccacctg tgtaggtgta aatatcgaga gtgcttcctg tttcaatctt tagtatagca
     2281 caacttctat tttggtctga agttatagta ttcccattac caataataac aatatccccg
     2341 gcgccgggta ctgtccctgc ggggctaccg gagtgaccgg tggtggacca gctgttcacg
     2401 tcgccccaca accccgtctg ccggctgtag taagtagttg gtgttccgaa ggggttggtg
     2461 gggttgttgt cgccggcggt gtagtcgccg tcaatgaacg caaccccctc caggaacgag
     2521 ccactacccg gttcgccaat aatgttgttg gtttcgtcaa catcgttggc agctccgctt
     2581 gtccacgtgc tggtggcggt gttgaagcgt gcggccacat agccatcctc ggtaacattg
     2641 cctacagcta ccacgtcggc ctgcaggtag tcgtaccggt gggtgagggt ggcgcttcca
     2701 agcgtgaatc ccgacgattt aaccctccag aagtaggtga gcgaacggct cttgactgtt
     2761 gtggccgggt gctcgccctc cacggggtaa acggtgatat cgccccatgc ggtaggcgtg
     2821 ccgttcacgg taagggttga gggggtgtag ccgttaatac caacgggata tacgaacgtt
     2881 gctgagccag cgctgtaggt gcgggtaatc ccgccatcac cggcattacc ggcagttttg
     2941 atgtagcggc tggagctgta ccccgaaatg gaggcggttg atgtcagctt caggttgtag
     3001 gtggagatgt cgagcagctt atcgcgggca aaggtgagca cccccgaaat ggtggtgttg
     3061 gccctgaggc tcaccggagc ggcaagcgca tcggagttgt tcagctccag gttcttgaat
     3121 atgccgcttc cgctgccgtc aaccaccgtg gggtcatcat cggcaatggc aattttccct
     3181 gttcccaggt gcagagcgct gttgaaaacg tacgatgtgc tgctggtagc agaggttacc
     3241 aggctaagcg tttttccgcc gtcgttcagg gttccggaca ccagcatcag cgagtcggcc
     3301 acctgaaagt cggtttgagc cccggccagg ttgagaattt tccctgcggg cttgtccatc
     3361 ctgaacttct tgaaaaccag ggctgaagcg gtgttagcgg ttatcgtctg gttggctgag
     3421 ccgttgaagg ttgtccagtt gttgcccgac gtgtaggtgg tgccattggc aacaaacatg
     3481 tttccgccaa tggtaacgtt ctggctgttg gcgttaaact cgcccgagag gagcgagtag
     3541 ttggccagca ccgtaagggg gtagccggtt accatggtaa ctactgcaac gctgctctga
     3601 cggttaacgg taaagttacc aaactcggca ttcgaggtga tgctgaagat gggcgagtcg
     3661 gcaagaccgg tgcctgtggt gggcataacc tccagggtgc caccggttac gttgatgttg
     3721 tttttcgacg acagtatttc aaccgctttt tgctggttgg cagcagtgcc atcgccgcaa
     3781 acatcaaaga ttctaattgt tccaccgctc atgttgaaca cgttggtggt ttcgttcagg
     3841 ttgaaagtgc caacaagtgc ttgcaggcca ccgctactcc ggttggttga aatgggggca
     3901 ttcaccaggt cgtccaccga ggtgtaggcc gagggcgttc tccaaaagcg acccctaagt
     3961 tcgaacagcc cgccggtttg ggtgtacgag gccagccctc ctgcggttcc cgcagtgcgg
     4021 tactgcttgg cgtcaaccct tccgccgttg atttcgaact gtcccgatga ttggctccac
     4081 tgtatgaatc ctccgctctc acgggttgat atatacccct tgttcaccct tagcagccct
     4141 aaggtggaga acgagctgca gccagcaccg gcatttactc ccattgtagc attgtcaggt
     4201 gcgctaactc cgtaggccac gttcacctcg cggtagtcgt cggcagtggc aagcaccacc
     4261 acgttgtcgc catccaaaac cagcgccccg ttggcaggaa cgtagaaatc ggagttaggc
     4321 cctcccgtgc cctggtcgca gttcccctcg ctcagcgagg gaataatggt gctgccctgc
     4381 agcaccatgg ttccgtttct tatccaaagc gccttacaca ggttggggtt tgcgcctccg
     4441 ccgtaacccc cggcattatt agccccaaat agcctgaagt tggcgtaggc ggtggagtaa
     4501 accgtaagcg agtaggtttg gtcaaccccc ttgtcgagca ctaggttgta gaagtcggtt
     4561 gtgccgttgc agtaaaggtc gttgctcgaa gcacccacaa agtaaacggt ggcaaaacct
     4621 gttgtgggcc ccagggtagt ggggggaaac gcgttgtaaa caggatagct gaggttggtg
     4681 aacctaaccg tgccgttgtt ggtgaggttt ccgtatatca ccacccggtg cgagtgggca
     4741 tcgtagtagg taatgtaggg tgccgttccc gattcggccg ctgtggttgg gtcggtaatg
     4801 gtgttggtaa cccctgtgcc aacggtaatg gaggcgcccg catcaacggt tacatcgccg
     4861 tgaatgatca gctgtcggcg gttggccgag ttgtcgttta tccggtaggt tccctgctta
     4921 acgtgcaggt tgccgttgag ggtgatgttg ctgagctggg tggctgtaaa acccggggca
     4981 ttaattctta ggtggttata ggtggtttgt gctgcgggga gggtgaagtt ggcggagttg
     5041 tagtactccg tggttccccc gccggcgttt acaaaggtat tggtggtagc tgtggggaag
     5101 ttaacggagg ccagcttaag ggtgccacct ccatcgagct ccttaagccc gcttgaaaag
     5161 gagtaggtag ccatatcgag aattcccccc tcgttgatgt tcacctccag ctgaacgttg
     5221 ctcacgttgg aggccaggga aaccgtgcgg tcgggcagga taaccacaac gctgctgtcg
     5281 gttggtgtgc caatgttctc ataggttgtt cctccggggt cgctggtcca ggtggaggca
     5341 tcgttccagc tgcccgactg gtgggagaag aaggtcttgg gcgggtagcc ggctgtccat
     5401 tcggtggtgg cagctgaaat attagtggtg cctgtaatgg tgaatttcct gttaataccg
     5461 tcgaacgatt gcgtgccggt aggctgcgac catgccccgc cattcctgta ccaaatctta
     5521 gtagggtcct tggggttttc ggttgggtcg tagccaaagg taattgtggc ggtggtagct
     5581 gccgaaccgg taagctgcca gtagcgtttc aggtactgcg agccaagcga ggtgctgtaa
     5641 accgtacgga aacgcaggta ggtggaaccg gaaatgcttt gaacggtaac gggtgcatag
     5701 gtgccgttgg agccgatggg aacggtgtag ggagttgcag gcacccgttg ctgcaggtag
     5761 ccggtgccgt tggtctccac catgcaggtg gagctgaacg gcccgccaag tatgttgctg
     5821 ctagccgcag tagccaaggt taggttgttg cctcccaact gcaaaacgcc tccgttcagc
     5881 gttagggtac tgtttacggt tgtgttgccc gtaagcgact tgttgccgct acccgagatg
     5941 gtaaggttgt agtaggtgta ggcccccacg ttctggttgc cgctgttgtt gtagttcacg
     6001 gtacctgcgg ttggggcacc ggtaccacca ccggctgtgc tggttattcc tcccccggaa
     6061 atggtgccct gaacgttaac cgttccgcca ccagaaaaaa gaatgtaat
//
LOCUS       DAOSHM010000101         5900 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_11898_length_5900_cov_21.837639, whole genome shotgun
            sequence.
ACCESSION   DAOSHM010000101 DAOSHM010000000
VERSION     DAOSHM010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 5900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5900
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11898_length_5900_cov_21.837639"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            357..2456
                     /locus_tag="PK839_10090"
     CDS             357..2456
                     /locus_tag="PK839_10090"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOG73124.1"
                     /translation="MATLEKIRNRAGVLVAVVIGLALLAFILGDLLSSGGSLFNRAQM
                     EVAKIGGTSIPFEMLQAKIDESENLQKMFSNQLSLDEQTVLRIREQVWQDLVRTYTME
                     DQYKKLGLSIHPDELFDMVQGRNIHPIIQQQFSDPNTGQLNKEFITMFLKNMDQDPRS
                     KTYWLFLEQEIQKDRLFNKYFNLITKGLYVTSVQAQRALEDRTKKVNFDYTLVAYSSI
                     PDSSISVEGKEIKNYYKSHLSEYKQTASRDIEYVVFPIQPSESDRQAAEEWINKMKDE
                     FLLADDPNQYVTLNSDSPLDSKYYKESELTDEMGKWAFSAKVGESYGPTFDGNSYKII
                     RLVNVKMLPDSVKARHILISPKAQNQEAVAKAKATADSLLNVIKRGGSWDDLASHFSD
                     DPGSKDKGGDLGWFPGGVMVKNFDEAAFNLPKNEVKVVETNYGFHILQVTDRGKESRK
                     VQLATLERKVVPSSTTSQRIYSEASQFASSNRTYEQFKTAADAKKLTRRVASNLLAND
                     QNIAGLESPREVIRWAFKAEEGDVSNVFELANMYVVASLTAARSEGVAPLKQVVADVR
                     TKVIRDKKAEKLAARVSDALKGASDIFSVAGKLNGKVEHAENVTFSSFTLPYAGFEPA
                     VIGVASVSSEGKLQGPVNGNAGVYVLAVTSIVTDQGDAGMEKLRLANTYQSRAYYEAF
                     EALKSKIGIVDKRYNFY"
     gene            2614..2988
                     /locus_tag="PK839_10095"
     CDS             2614..2988
                     /locus_tag="PK839_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOG73125.1"
                     /translation="MKVAVPTRNGVVDEHFGHCEAYTVFTIDSGNKIENSEILPSPQG
                     CGCHSNIAATLKEKGVEVMLAGNMGDGALNMLAGHGIQVFRGCSGDARRLVENFLSGN
                     VEDSGEGCHHHHEHEEGHTCNH"
     gene            complement(3152..4813)
                     /locus_tag="PK839_10100"
     CDS             complement(3152..4813)
                     /locus_tag="PK839_10100"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOG73126.1"
                     /translation="MIKYTFLRFVKKLKHEKYLLFSIFIVLCTLLLFINVGTYGVVES
                     SDARYAEISREMLESGDYIHPTLLNIQHYHKPPLTYQLTALGYQIFGVNELGARFFLQ
                     LSLLIQLLLVYALSNILFGNRGTALWAAIIYFSFPLTLISTRNLTTDSFLATFVLLSI
                     YSWVKYRKYGKFRYLYLTAISLALGFYTKGPVVLLVPLVFIIAYNRIEKSKNRFGVHH
                     LLSLVVFLGISLWWFVFLIRENGAFADYFLGRQTVERFGKDVFNRSEPFWYFVAFAPL
                     VGLPWLLILPYLMVQNRKSILKKGINSVLTLSFVIPLVFFSISTSKRILYILPFYSIL
                     AMLTANLLANLISSKEKVIDRSVTIFSMIVLLVATATMFIHTNYVIPKGVGIASILLI
                     VVILAIYRYLQGGRKFKPVLVSFLVSGFLLITSGLLMQKNELEFNISRPISNFIKSKN
                     LQDRNILIYNTMKPSIAFDMRKPVISLYDGSNTLAREVQFEENSAWKSYFYDLTDSID
                     TNRLKGLMERPSVLVSYKHPLPENRQWLAKRFSTRKDIGKWHIYY"
     gene            5189..5851
                     /locus_tag="PK839_10105"
     CDS             5189..5851
                     /locus_tag="PK839_10105"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HOG73127.1"
                     /translation="MTTLEKVLELKAKVKHLMNAEVADAMRREGIIYKVNYGVSIPEL
                     KALAKPYLGDHELALELFREEIRECKLLATMIDDPELITGEQLDEWANDFTNPEIVEQ
                     VCGNLFWRTGYALSRSIEWCLDDDELFQKAGLLIIARKASDPNVGVNLLQPYIGVIEN
                     MADTMTDTILSSMSFALREIAKRSPELCGMVLSAAQRMTQLDNELAAWLGNELIYELT
                     EA"
BASE COUNT         1700 a         1201 c         1401 g         1598 t
ORIGIN      
        1 ctacgttccg cccccgaccc atttgcgggt caggctttta tgccatttac ttataccagt
       61 aacgttcaac gattcccgcc tttccgtgca ccgctgagaa tacctttccc cagctggggc
      121 tgaagacatg gagaaagttg aagggtaaag gataaggtta acttttcccg gtttggtaaa
      181 aggcaaacga tttacgattc tatcctttat ccttgaacat cttgactcac tttctctctc
      241 ccttttagtt aacccccata taaaaaaatt gcttggtgct taaagcccta ttgctaaaat
      301 tgctatattc gtggcttgaa ttttagctga atttctataa tttaaatatc atttaaatgg
      361 ctacactcga aaaaattaga aaccgggcag gtgttcttgt tgcggtggtg attggtttag
      421 cattgctggc attcattttg ggcgacctgt taagttcagg cggatcgcta tttaaccgtg
      481 ctcaaatgga ggtcgctaaa attggtggca cttctatacc tttcgaaatg ctgcaggcaa
      541 agattgacga gagcgagaat cttcagaaga tgttttccaa ccagctatca ctcgatgagc
      601 aaacggtgct gcgtatcagg gagcaagttt ggcaggattt ggttagaaca tacaccatgg
      661 aagaccagta caaaaagctg gggctctcca ttcaccctga cgagcttttt gatatggttc
      721 agggaaggaa catccatcca ataatccagc agcagtttag cgatccaaat accggccagc
      781 tgaacaagga gtttatcacc atgtttctta aaaacatgga ccaggatccc cgctccaaaa
      841 catactggct ttttcttgag caggaaattc agaaggatag gcttttcaat aagtacttca
      901 accttattac taagggctta tatgttacct cggttcaagc ccaacgcgca ttggaggata
      961 gaactaaaaa ggtaaatttc gactataccc tcgtggccta ctcttctatt cccgattcct
     1021 ctatttccgt tgagggaaaa gaaataaaaa attactacaa atctcaccta agcgagtaca
     1081 agcagaccgc ctcaagggat atcgaatatg tagttttccc cattcaacca tccgaaagcg
     1141 ataggcaggc tgctgaggag tggattaata agatgaagga tgagtttcta ctcgctgacg
     1201 atccaaacca gtatgttacc ctcaactccg atagtccact cgactcgaag tactacaagg
     1261 aaagtgagct gactgatgag atgggaaaat gggctttttc tgccaaggta ggcgaaagct
     1321 acggccctac atttgacgga aattcctata aaattatccg gctggtgaat gtcaagatgc
     1381 tacctgactc ggtgaaggcg cgtcatatac tgattagccc taaggctcaa aatcaggagg
     1441 cagttgctaa agccaaggca acagcagata gcctgctgaa tgtaattaaa cgtggaggga
     1501 gctgggatga tcttgcctca catttttccg atgacccagg atctaaggat aagggtggcg
     1561 acctagggtg gtttcccggc ggtgtcatgg tgaaaaattt tgatgaggct gcattcaacc
     1621 tcccaaaaaa tgaggttaag gtagttgaaa ccaattacgg atttcatatt cttcaggtaa
     1681 ccgatagggg gaaggaatcc aggaaggtgc agctggctac tctggagcga aaagtagtac
     1741 caagtagcac caccagccag aggatataca gcgaagcttc gcaatttgcc tcatctaaca
     1801 gaacctacga gcagtttaaa accgctgctg atgccaaaaa acttacccga cgggttgcct
     1861 ccaacctgct ggccaacgac caaaatattg ccgggctgga atcaccccgt gaagttattc
     1921 gctgggcatt taaggctgaa gagggcgatg tttcaaacgt gtttgagttg gccaacatgt
     1981 atgtggttgc ctctcttacc gctgcccgta gtgagggagt tgcaccctta aagcaggttg
     2041 ttgccgatgt aagaaccaag gtaatcaggg ataagaaggc cgaaaagttg gcagcacgtg
     2101 tgagtgatgc tttaaagggc gccagcgata ttttttccgt tgcgggtaag cttaacggca
     2161 aggtagagca cgccgaaaat gttacattta gctcgtttac tttgccctat gcaggttttg
     2221 agccagctgt tattggagtg gcttcggtta gctccgaggg taagcttcag ggacctgtca
     2281 atggtaacgc cggggtttac gttttggctg ttacatctat agttaccgat cagggagatg
     2341 ccggaatgga aaagttaaga ttggccaaca cctaccagtc cagggcatac tacgaggctt
     2401 ttgaggcgtt gaaatcaaag attggcattg ttgataagcg ctacaacttc tactagacag
     2461 catcgaacct gaaagttgtt cactactgag gggaaataaa atttcccctt ttttgcatca
     2521 ctgttttttc gaactaaaag tttgcaaaat ggttataatt gaacaggcaa aacgaaaatt
     2581 atcggaatta ttaaccaaat aaaatgaaat gttatgaagg ttgctgtacc aacaagaaac
     2641 ggtgttgttg acgaacactt tggccattgt gaggcttaca ccgtattcac gattgactcg
     2701 ggtaataaga ttgaaaactc agaaattctt ccatcgcctc agggttgcgg atgccatagc
     2761 aatattgctg ccactctgaa ggaaaaaggt gtagaagtta tgctcgcagg gaacatgggc
     2821 gatggtgcat tgaacatgtt agctggtcat ggaatacagg tatttcgtgg ctgttcgggc
     2881 gatgctcgcc ggttggttga aaatttcctg agcggaaatg ttgaggattc aggcgagggg
     2941 tgtcatcatc accacgaaca tgaagagggt catacatgca atcattagca agtatattgg
     3001 ctattcggag ctacattaag tgaggctttt ttaccctggg ctatgaataa tcgttgagaa
     3061 acattaccag tggtaatttt ttgtggcgat aaatccggtt ctgcaccgaa accgccttta
     3121 taattttatt tgccttcacc ccctttcagg gttagtagta gatgtgccat tttcctatgt
     3181 cttttcttgt gctgaacctt ttcgccagcc attgcctgtt ttcaggtagg ggatgcttgt
     3241 aggaaaccag aactgacggc ctttccatca ggcctttaag ccgattggta tcaatgctgt
     3301 cagtgaggtc gtaaaagtag cttttccatg ccgaattttc ctcaaactgc acctcccggg
     3361 caagagtgtt cgaaccatca tatagcgaaa tcaccggctt tcgcatgtcg aaagcaatgg
     3421 agggtttcat ggtgttgtag atgagtatat tcctgtcctg tagatttttg cttttaataa
     3481 agttggagat tggcctggaa atgttgaatt ccaactcatt tttttgcatc agcaggccgc
     3541 ttgttataag aagaaacccg gaaaccaaaa acgagacgag cacaggtttg aattttctac
     3601 ccccctgtaa gtaacgataa attgccaaaa tgactacaat tagtagaatg ctggcaatgc
     3661 ctactccttt tggtataacg taattggtgt gtataaacat ggttgccgtt gcaacaagta
     3721 ggacaatcat tgagaatatc gttacgcttc ggtcgataac cttctctttt gagctaatta
     3781 gatttgccag caggttggcg gtgagcattg ccaaaatgct gtagaacggc agtatgtaca
     3841 gaattctttt tgaggtagat attgaaaaga acaccagtgg aattacgaag cttagcgtaa
     3901 gcacagaatt tatacccttc ttcaggatgc tttttctgtt ctgcaccatt aagtagggta
     3961 gaatgagtag ccatggaaga cctacaagcg gtgcaaaagc tacgaagtac cagaatggct
     4021 ccgaacggtt gaaaacatcc tttccaaacc gttccaccgt ttgtctgcct aaaaagtagt
     4081 cggcaaacgc tccgttctct ctaatcagga aaacaaacca ccatagcgaa atacccagaa
     4141 aaaccaccag ggaaagtaga tggtgaactc caaaccgatt tttacttttt tcaatgcggt
     4201 tgtaggctat aataaatacc aatggtacca gaagtaccac cggaccttta gtatagaatc
     4261 ccaaagccag gctaattgca gttaagtaga ggtaccgaaa tttgccgtat tttcgatact
     4321 taacccagga gtatattgac aacagaacga aagttgctaa aaacgaatcg gttgttagat
     4381 ttcgtgtaga tatgagagtt aatgggaagc tgaagtatat tatagctgcc cataatgcgg
     4441 taccacggtt gccaaagaga atgttggata gggcatacac taaaagcagc tgaataagga
     4501 gggagagctg caggaaaaaa cgggcgccaa gctcgtttac gccaaaaatt tggtaaccta
     4561 aagcggtaag ctggtaggtt aacgggggct tatggtagtg ttgaatattc aaaagggtgg
     4621 gatgaatgta gtcgcccgac tctagcatct cacgcgaaat ttcagcgtag cgggcatcgc
     4681 tactttcaac aaccccgtag gtaccaacgt tgatgaacag aagcaaggtg catagaacaa
     4741 taaaaatcga aaatagcagg tacttttcat gctttaactt tttaacaaac cgcaggaacg
     4801 tatatttaat cattgatagc aacgttaatc cggaatcagt gcgtccagac tttgcctaga
     4861 aactaacttc aaaatatcat aacactatgc ttatggtgaa ggttcaccca tgttattttt
     4921 ataagtgtaa aggagcatta caattttcta ttgatacact gttggacagt taactttgct
     4981 gccagctgga cagaatggca tggattatta ctgtgttaat aggtaattta agggctattt
     5041 gttaatattt tgatatttta cacctagaag taagcaatct tttttaattc cgtttgctgt
     5101 aggtaatggt tttgagtggg cagtaggggt tgagaactca ttatgggttt agtagaaaaa
     5161 atgctaattt tgaaattaaa atagctgtat gactacgctt gagaaagttc tggagttaaa
     5221 ggcgaaggtg aaacatctta tgaatgccga ggtagcagat gccatgaggc gcgagggtat
     5281 tatctacaag gtaaactacg gcgtatccat tcctgagctt aaagcgttgg caaagcccta
     5341 tttgggcgac cacgagctgg cgctggagct attcagggaa gagataaggg agtgcaaact
     5401 tctggcaacc atgatagatg acccggaact tattaccggc gagcagctcg atgagtgggc
     5461 aaacgatttt accaaccctg aaattgtgga gcaggtgtgc ggtaaccttt tctggagaac
     5521 cgggtatgcc ctatcgcgca gcattgagtg gtgtctggat gatgatgagc tgttccaaaa
     5581 ggccggattg ctgataattg cccgaaaagc ttccgatccc aacgttggcg taaacttgtt
     5641 gcaaccctac attggcgtta ttgaaaatat ggccgacacc atgaccgata ctattctttc
     5701 gtccatgtcg ttcgccctac gtgaaattgc caaacgctca cccgagcttt gcggtatggt
     5761 gctttcggca gcccagcgga tgactcagct ggataatgag cttgcagcat ggttaggtaa
     5821 tgaacttatc tacgagctca ccgaggccta gactattgga aaagacaagg ataaagcata
     5881 agataaactt gtccgcctcc
//
LOCUS       DAOSHM010000102         5854 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_12032_length_5854_cov_19.785825, whole genome shotgun
            sequence.
ACCESSION   DAOSHM010000102 DAOSHM010000000
VERSION     DAOSHM010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 5854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5854
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12032_length_5854_cov_19.785825"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(120..1040)
                     /locus_tag="PK839_10110"
     CDS             complement(120..1040)
                     /locus_tag="PK839_10110"
                     /inference="COORDINATES: protein motif:HMM:NF026253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HOG73128.1"
                     /translation="MKKILLLLLAAIITLTSVGQGTVKKGKASTGPYPRGWVLVEKGG
                     KYGFISAEGDEIIKTQYTRISPFDEYLSGWALVEKDGFLGFISNSGDEVVKPQYTKIY
                     PFDEMQRGWAMVEISGLFGFISSEGREVVKPKYSKIYHFGEMYKNLAMVEKDGLVGFI
                     NLDGSEVLKPQYQKVHPFGEYKKDWAMVERDGFFGFINGEGKEVVAPKYSKVSPFGEY
                     QKNWALVEKDRYLGFIDMDGREVIKPQYSQILPFGQLQKDWAMVERDGLWGFISADGR
                     EVVKPKYEKINLDDVSGIKKTNEVVKDENS"
     gene            1444..2823
                     /locus_tag="PK839_10115"
     CDS             1444..2823
                     /locus_tag="PK839_10115"
                     /inference="COORDINATES: protein motif:HMM:NF013395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HOG73129.1"
                     /translation="MKRIKILHLAMQVAIAALLLSVSACEKEYPTINSNEQTAVVQDD
                     VQKATATFAENFESGTKTSYTGASVTLATGSWYLNDALLGNSSSDRKNGTKSVRIRNT
                     GKLTMQFDKTTGAGTVQIKHAKYGSDGSSTWELYISTNSGSTWSKVGSTVTTSSTTLT
                     AASFTVNVSGNVRFEVRKISGGSYRINIDDFSITDYGSTPPPSSDPTRDNNMALGNPS
                     GAVTSTAYPNNYLMNKTQYSLSYNNSKLTCNWVSWHLSTAWLGSTPRQDDFRADATLP
                     SGWVQVGGSDYSGSGFDRGHMCPSADRTGSVTDNSATFLMTNMVPQAPNNNQITWANL
                     ENYCRTLVNAGNELYIISGPWGQGGTGSNGTKSTISSKNIVVPSYVWKIIVVLPVGSN
                     DVTRITSSTRVIAVWMPNTQTVSSQSWGYYRTTVDYLESKTGYNFLSNVSTTIQSAIE
                     SKVDNGPIN"
     gene            complement(2898..4172)
                     /gene="serS"
                     /locus_tag="PK839_10120"
     CDS             complement(2898..4172)
                     /gene="serS"
                     /locus_tag="PK839_10120"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015547591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOG73130.1"
                     /translation="MLTLKQIRENKEEVIRRLAVKHFDGAAIVGKIIELDDRRKAIQL
                     QLDTNLAEQNSLARQIGKLFAEGKSVEANDAKARTTNLKEQSKLLTQQLAGVEAEQRQ
                     LLLQIPNLPHDSVPKGVGADDNVIVRSGGEIPELPGDVLPHWDLAKKYDIIDFELGVK
                     ITGSGFPVYKGKGAKLQRALINFFLDCNTAAGYQEVEPPHMVNEDSAMGTGQLPDKDS
                     QMYHIGLDGFYMIPTAEVPLTNMFRDVILTSSMLPVKVTAYTPCFRREAGSYGKDVRG
                     LNRLHQFDKVEIVQVQHPDKSYQSLEEMVLHVEGILKKLELPYRIVKLCGGDMSFTSA
                     LTYDFEVFSAAQKKWLEVSSVSNFESFQANRMMLRFKDDGDKRTQLAHTLNGSSLALP
                     RIVAALLENNQTPNGIRIPAAIKNYCGFDLIS"
     gene            complement(4262..4522)
                     /gene="rpmA"
                     /locus_tag="PK839_10125"
     CDS             complement(4262..4522)
                     /gene="rpmA"
                     /locus_tag="PK839_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HOG73131.1"
                     /translation="MAHKKGVGSSRNGRESHSKRLGVKLFGGQVAKAGNIIVRQRGTK
                     HNPGQNVGMGRDHTLYALVDGEVSFRKKTDNRSYVSVIPKEA"
     gene            complement(4543..4854)
                     /gene="rplU"
                     /locus_tag="PK839_10130"
     CDS             complement(4543..4854)
                     /gene="rplU"
                     /locus_tag="PK839_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HOG73132.1"
                     /translation="MYAIVDIVGQQFKVEKDQKLFVNRMAGETGTEVKFDKVLLVDND
                     GAVTVGKPQVENASVTAKILRHVRGDKVIVFKKKRRTGYKKKNGHRQNYTEIQISEIV
                     A"
     gene            complement(4982..5761)
                     /locus_tag="PK839_10135"
     CDS             complement(4982..5761)
                     /locus_tag="PK839_10135"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HOG73133.1"
                     /translation="MQIFKSTLLVFATILLWIIALASCIVFFAGDVVVWFLTFWWDKR
                     LWLLHRYSILWAMFYLKINPFWRVRFEGKENIRKEQVYVIVSNHQSAFDIILLYRLYT
                     HFKWVAKKELAKVPFIGWNLLLNNHIFIERGSAVSAKKMMEQGMKHLRMGSSLLIFPE
                     GTRSADGTIHRFKDGAFILAQKSEKPILPVVIDGSGEIMQNPLKLKLAQTLTIKILPE
                     ISYESFREFSPNELARHVQQLMEKEHMAIAPERYHGSMVNS"
BASE COUNT         1647 a         1468 c         1141 g         1598 t
ORIGIN      
        1 ggattacaaa aagcgtgaag cagctttcgg aaaattttcg caaatgggca accaatatag
       61 ggtttgctga gaaagtcagc aaaccctata tatgtcaagg tctaaatacg aatcttcgct
      121 cagctatttt cgtcctttac aacctcgttg gtttttttga tgcccgaaac atcatcaagg
      181 ttgattttct cgtacttcgg cttcaccacc tcccggccat cggctgaaat gaaaccccaa
      241 agcccatctc gctcaaccat agcccaatcc ttttgtagct gaccaaacgg cagtatctgg
      301 ctatactggg gtttaatcac ctccctgcca tccatatcaa taaaacctaa gtagcggtcc
      361 ttctcaacaa gcgcccaatt cttttggtac tccccgaatg gagaaacttt actgtacttt
      421 ggagcaacaa cctctttccc ctccccattt ataaagccaa aaaatccatc gcgctccacc
      481 atggcccaat cctttttata ctcgccaaat ggatgcactt tttggtactg gggcttaagc
      541 acctcgcttc catctaaatt tataaaacct accaacccat ccttctccac catggcgagg
      601 ttcttataca tttccccaaa atggtaaatc ttgctgtact ttggtttcac gacttccctg
      661 ccttcgcttg atatgaaccc aaacaacccg ctgatttcga ccatagccca gccacgctgc
      721 atctcatcaa aaggataaat tttggtgtac tggggtttaa caacctcatc acccgagttg
      781 gagataaacc ctaaaaagcc atctttctct accagcgccc atcccgacag gtactcgtcg
      841 aagggagata ttctggtgta ctgggtttta ataatctcat ctccttcggc cgatataaac
      901 ccgtacttgc ctcccttttc aaccagcacc catccacggg ggtatggacc tgttgaagct
      961 ttaccctttt tcacggttcc ctgcccaacc gaggtaaggg tgataatggc agcaagcaaa
     1021 aggagtagaa tttttttcat atcaagaggt ttatgcgtaa atatgaatct cggtttccca
     1081 atacttaaca gatacaaacc tactgaaatt ttttatgata caccaaagag gtaagggtaa
     1141 ttctgcccca caaccttaaa aagtcatggc caaaagttaa tgcggctaat aaattttacc
     1201 gcattcccgg gatgactcgt taaaaaagtc aataattaaa cggagaaccc gtaattccaa
     1261 ctaaataggg catttataag acattggaat aaaggcatgt agaaaattca aacggctaaa
     1321 cctaattttc cagcatttat aacatattgt taacactatc caaataaaat aggtgcattt
     1381 ttgctactta agcaaacaaa actatttcat aattttattg caccaaccta aacctattta
     1441 accatgaaac gaattaaaat tttacatctc gccatgcaag ttgcaatagc tgcactgctc
     1501 ctgtcggtta gtgcctgcga gaaggagtat ccgaccatta attccaatga acaaacggct
     1561 gttgtgcagg atgacgttca gaaggctacc gctactttcg ccgaaaattt tgaaagtgga
     1621 acaaaaacct cctatacagg tgcatcggtt acccttgcta caggaagctg gtacctgaat
     1681 gacgccctgc taggaaattc atccagcgac aggaagaacg gaacaaaatc tgtaaggatt
     1741 aggaacaccg gcaagctcac catgcagttt gataaaacaa ctggagccgg aacggtgcag
     1801 attaaacatg ccaagtacgg cagcgatggc agcagcactt gggagcttta catctcaacc
     1861 aacagcggta gcacatggag taaggtgggc tcaacggtaa ccacctcatc taccactcta
     1921 actgctgctt cctttacagt taacgtatcg ggaaatgtac gtttcgaagt aagaaaaata
     1981 tccggcggtt cgtaccgaat aaacattgac gatttctcaa ttactgacta cggctccact
     2041 cctcctccat cgtccgatcc tacccgggat aacaacatgg ctttaggaaa cccgtcgggt
     2101 gcagtaacca gtaccgccta ccctaacaac tacctgatga acaaaacgca gtactcccta
     2161 tcatacaaca acagcaagct aacctgcaac tgggtgtcgt ggcatctgag cacggcatgg
     2221 ctgggtagta cccctcgtca ggatgatttc agggccgatg ccacccttcc ctcgggatgg
     2281 gttcaggtgg gaggaagcga ctacagcggc tccggctttg accgcggcca catgtgcccc
     2341 agcgctgacc gcacagggtc ggttaccgac aactccgcca ccttccttat gaccaacatg
     2401 gtaccccaag ccccaaataa caaccagatt acctgggcaa atttggaaaa ctactgccgt
     2461 acactggtta atgcaggtaa cgagctctac atcatatccg gcccctgggg tcagggtgga
     2521 acaggctcta acggcaccaa gtcaaccatc tcatctaaaa atatcgttgt tccctcatac
     2581 gtttggaaaa ttattgttgt gctccctgtt gggtcaaatg atgttacgcg tatcacctca
     2641 tcaacaaggg ttattgctgt ttggatgccc aacacccaaa ccgtaagcag ccagtcatgg
     2701 ggctactacc gcactacagt tgactacctt gaaagtaaaa caggatacaa tttcctttcg
     2761 aatgtatcaa ctacaattca aagcgccata gagtcaaaag ttgacaatgg cccgataaac
     2821 tagagaggag taattctccc ttttacaaat gaaaaagccc tgcaacccag ggcttttatc
     2881 atatttctaa acaaacctta gctaatcaaa tcaaacccac agtaattctt aattgcagcg
     2941 gggattctta ttccgttagg cgtttggtta ttttccagca gagctgcaac aattctgggt
     3001 agagccaacg agctgccatt gagggtgtgg gctagctggg ttcttttatc accatcatcc
     3061 ttaaagcgga gcatcatgcg atttgcctga aacgattcga agttggaaac cgagctcacc
     3121 tcaagccact ttttttgtgc ggctgaaaac acctcaaaat cataggttaa cgccgaagta
     3181 aagctcatat cacccccgca aagcttaaca atacgatatg gaagctccag ctttttaaga
     3241 attccctcaa catgcaaaac catttcctct agcgactggt aagatttgtc gggatgctgt
     3301 acctgaacaa tttctacctt gtcgaactgg tgcaggcggt taagccccct aacatctttt
     3361 ccataggagc cggcctcacg tctgaagcat ggagtgtagg ctgttacctt aaccggaagc
     3421 atggaagaag tcaggataac atccctgaac atattggtaa gcggtacctc cgcagtagga
     3481 atcatgtaga aaccatccag gccgatgtgg tacatctggc tatccttgtc gggtagctgg
     3541 ccagtgccca tggccgaatc ctcgttgacc atgtggggtg gttcaacttc ctggtagcct
     3601 gcagcggtgt tgcaatcaag aaaaaagttg ataagcgccc gctgtagctt tgcccctttc
     3661 cccttgtaaa ccgggaatcc gcttccggtg atttttacac ccagctcaaa gtcaattatg
     3721 tcgtattttt tggctaaatc ccaatgtgga agcacatctc ccggtaattc gggtatttca
     3781 cctcccgaac gaacaataac attatcgtcg gcaccaaccc cttttggtac agaatcgtgc
     3841 ggcaaattag gtatctgaag tagaagctgc ctctgttcgg cttccacccc ggccagctgc
     3901 tgcgttagta gctttgactg ctccttaagg ttggttgttc tagccttagc atcatttgct
     3961 tcaacagatt tgccctcggc aaaaagtttt ccgatctgac gtgcaaggct attttgctcg
     4021 gccaggttgg tgtcgagttg tagctgaatt gccttccgtc tatcatccag ctctataatt
     4081 ttcccgacga ttgcagctcc atcgaagtgc tttacagcca aacgacggat aacttcctcc
     4141 ttattctcac gaatctgctt cagtgtaagc atgacggcat attttaaaat aacaaaaaat
     4201 cctgcacaac acattcagca ttgagcagga gatcaaagtt aaaagtattt ttttacctac
     4261 ctcaagcttc tttggggata accgatacgt atgacctgtt gtcggttttt ttacggaagc
     4321 tcacctcgcc atctacaagg gcatataggg tatgatcgcg tcccatccca acgttttgac
     4381 cgggattgtg ctttgttcca cgctggcgaa caataatatt accagcctta gcaacctgcc
     4441 ccccaaaaag tttaacaccc aggcgtttac tatgcgattc cctgccattt ctcgaactac
     4501 caactccttt tttgtgtgcc atgattcgct acctttaaaa tgttaagcta cgatttctga
     4561 tatttgaatc tcggtgtaat tctgacgatg gccatttttc ttcttatagc ctgtgcgacg
     4621 ttttttcttg aaaacgataa ccttatcgcc tcttacatgg cgaagaattt tcgctgtaac
     4681 cgatgcgttt tctacctgag gctttccaac ggttacagcc ccatcgttgt caactagcag
     4741 taccttatca aattttacct cggtaccggt ttccccagcc atgcggttga cgaaaagttt
     4801 ctgatccttt tcaaccttaa actgctggcc tacaatatca acaattgcat acattatttt
     4861 cagaatgaaa tgattaacat attttgggct tgcaaaaata taaaagtttt actttacgac
     4921 aaatgttttg aaccattttt tttaatttga cttacctaca atttcaccaa agttaatatg
     4981 gctagctgtt aaccatactg ccatgatacc tttcgggagc aatagccatg tgctcttttt
     5041 ccattagctg ctggacgtgc cttgccagct cgttagggga aaattccctg aaactctcat
     5101 atgaaatctc cggtagaatt ttaatggtaa gggtttgcgc caactttagc ttcagggggt
     5161 tttgcattat ttcgccagaa ccatctatta ctacaggtaa aattggcttt tcagactttt
     5221 gggcaagaat aaaagccccg tccttaaacc gatgaatcgt accatcagca gaacgtgtcc
     5281 cttcagggaa aatcagcaag gagctgccca ttctcaagtg cttcattccc tgctccatca
     5341 tctttttagc gcttaccgca ctaccccgct caataaatat atggttgttg agtagcaagt
     5401 tccatccaat aaacggaact ttagccagct cctttttggc aacccactta aaatgggtgt
     5461 aaagcctgta gagcaaaatg atatcaaatg cgctttgatg gtttgatacg ataacgtaaa
     5521 cctgttcctt cctaatgttt tcctttccct caaaccttac cctccagaat gggttgattt
     5581 tcaggtaaaa catagcccat agaatggagt agcgatgtaa cagccacaaa cgtttgtccc
     5641 accaaaaagt tagaaaccat accactacat cacctgcaaa aaaaactatg caggaggcca
     5701 gtgctataat ccacaacaaa atggtggcaa atactagtaa tgtggatttg aatatttgca
     5761 ttgagatcac tagatttgaa aatattttgc aaaggtaacc ttaacattta aatcatccga
     5821 ccatgaaacc gttaaaagtt gcgcaacccc tagc
//
LOCUS       DAOSHM010000103         5799 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_12199_length_5799_cov_10.569116, whole genome shotgun
            sequence.
ACCESSION   DAOSHM010000103 DAOSHM010000000
VERSION     DAOSHM010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 5799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5799
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12199_length_5799_cov_10.569116"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(72..1235)
                     /locus_tag="PK839_10140"
     CDS             complement(72..1235)
                     /locus_tag="PK839_10140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG73134.1"
                     /translation="MVKKNILIVGDANSFLLHDIVSNICERNSNLVVDILNTNPSHLA
                     IDRNIFHKELLPCKLLLNIQKTTDKVNPYLSFFLMRFVIRRSLKQTGGKYDYIHLHFF
                     NKNFFYINISNFTNLTQNLIISYWGSDFYKRTDSQKQKMLPYINTASRIIFSNPQMMA
                     DFQAYFKGFEHKCMVQPIGLEILNYIDSLQESYSDIKGKAKNELCIPPEKLVVSIGYS
                     SHVEQHQVEIIEILAQRLSPAELESLFFIFPLTYGDTTYKDKVVDSARLHGLNFKAFT
                     STLLKQDVAKIRLLSDVFVHLRDTDQFSGSFQEYLYCRNIVITGKWLPYQFLADKGAY
                     FRRLDSIDDLPETLKQVVGNYENEYSKTTVNKEIIAGISHWKNIVDQYLTIYS"
     gene            complement(1275..2336)
                     /locus_tag="PK839_10145"
     CDS             complement(1275..2336)
                     /locus_tag="PK839_10145"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOG73135.1"
                     /translation="MATKQEKIEAIKIQQNASIVDALKIMDSTYRRLLLVFEGNRFIN
                     ILSIGDIQRAIIKNKPLNAPVSSILRKETLISTTLDSSEDISRKMLDQRIECMPIVDE
                     SQSLVDVIFWEDVFAEKEKPIKHSFNHPVVIMAGGEGTRLRPLTYVLPKALIPIGSES
                     MIERIINSFTGFGTSQFYISVNYKANMLRQFVDEKFGERFNISYLTEEKPLGTAGALY
                     SLKGVISGDFFVSNCDILIRNDYSEILDYHQSNHNDLTIVSALKHYSIPYGILETETD
                     GMLKRLTEKPEFTFQINSGMYILHSRVLNLIPTNKYFHMTHLIDSILTNSGRIGVFPV
                     SEKSWFDIGDWHNYLQNNI"
     gene            complement(2346..2933)
                     /locus_tag="PK839_10150"
     CDS             complement(2346..2933)
                     /locus_tag="PK839_10150"
                     /inference="COORDINATES: protein motif:HMM:TIGR03570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HOG73136.1"
                     /translation="MSQKEQIILVGGGGQCRASIDVIEQQGLYEIAAIVDIPSRVGQN
                     VLGYPISFTDDELPSLATNYKHFLITVGQIESPQPRLKLFRLLKQLNVSLPVIISPLA
                     YISPHSEIGEGTIIMHHVLVNAGAVIGKNCIINSKALVEHDTRVEDFCHISTGAILNG
                     EVTVGEQSFIGSNATLRQGVTIPVGSFIKANTLVK"
     gene            complement(2930..4099)
                     /locus_tag="PK839_10155"
     CDS             complement(2930..4099)
                     /locus_tag="PK839_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004289852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LegC family aminotransferase"
                     /protein_id="HOG73137.1"
                     /translation="MNQPKFDEIVDFIHSTYPGTGIIPLHSPVFKGKEKDYLLSCIDS
                     TFVSSVGQYVDKFEEVVATYTGSKKAVVTVNGTNALHLALLAAGVKHDDEVITQPVTF
                     IATANAIAYCNAHPVFIDVDRDTLGLSPEKLDSWLKLNTETRKNQNSNSYGLYNKHTG
                     RKISAVVPMHTFGHPCRIEDIVEICNQHNIPVVEDAAESLGSFYKGRHTGTFSKMGVL
                     SFNGNKIVTTGGGGMILTDDVELGKKLKHLTTQAKVPHPWEFYHDEVGYNYRMPNINA
                     ALGVAQMEQIEIFLKNKRETALRYKAYFESIGVNFFTEPVHSKSNYWLNAIVLSDRAE
                     RDSFLAYSNDKGIATRPMWTLMNKLPMFKNEQTDDLTNAQWLEDRVVNIPSSVRI"
     gene            complement(4086..4577)
                     /locus_tag="PK839_10160"
     CDS             complement(4086..4577)
                     /locus_tag="PK839_10160"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018710304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOG73138.1"
                     /translation="MKLLASIYHLLLKHHLKEKRREYLKKFDIDPTVRLPYLENTWFH
                     GNIHIGKNTYFNGGRIVTGPKSKVVIGEWCAIGHNVNIIAWTHDTEESTGPMNKRPTI
                     EKDIRIGNHVWVGTNVFIREGVTIGDNSIIGANSLVINDVEANSIVGGVPARLIKYKN
                     ESA"
     gene            complement(4699..5673)
                     /locus_tag="PK839_10165"
     CDS             complement(4699..5673)
                     /locus_tag="PK839_10165"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOG73139.1"
                     /translation="MQKVLVTGGAGYIGSVLVRQLLEHNYRVVVLDSLMYGGESLLDL
                     FNNNNFDFVKGDIRREEDIAMAMKGVDFVVHLAAIVGDPACAKQPELAKSTNLDGSKL
                     LYSIANKMGVKRFIFSSTCSNYGRMANPDIMVDEMSELAPVSLYAETKVGVEKFLLSQ
                     PKSNTCKPTVLRFSTVYGLSLRPRFDLTVNEFTKELALGRELVIFGEQFWRPYCHVVD
                     LARSVLIALEAPMEKVAFNVYNVGDSSENYQKKMIVEEIKKFIPDSKIKYIQKNEDPR
                     DYRVSFEKIKNELGFSITQRVPDGISQILKVIKQRILLNPDNAEFKNV"
BASE COUNT         1581 a         1187 c         1176 g         1855 t
ORIGIN      
        1 cgagcctacc actgtgtaga tgaacgaaga tttgatgaat gatttagagg gcatcctaaa
       61 tggctaacat atcaactata aatggtgagg tactggtcca ctatattttt ccagtggcta
      121 atcccagcaa taatctcttt attaacggta gttttgctat actcattttc ataatttcca
      181 accacctgtt ttagcgtttc aggcagatcg tcaatgctat cgagccttcg aaagtatgcg
      241 cccttatctg ccaggaattg gtaggggagc catttacccg tgataacaat attcctgcag
      301 tataaatact cctgaaagga gcccgaaaac tggtcagtat cccgtaggtg gacaaataca
      361 tcggaaagaa gtctgatttt ggcaacgtct tgctttagca acgtgctggt gaaagctttg
      421 aagttgagac catgcaacct ggcactatct actactttat ctttatacgt tgtatcacca
      481 taggtaaggg ggaatatgaa gaagagggat tccagctccg ccggacttaa cctttgtgcc
      541 aggatctcaa ttatttcgac ttgatgttgc tctacatggc tgctgtagcc aatgctaact
      601 acaagttttt cggggggaat gcacagctca tttttagctt tcccttttat atccgaatat
      661 gactcctgca ggctatctat ataattcaag atttctaacc cgataggctg caccatacac
      721 ttatgctcaa aacccttaaa gtaggcttgg aaatcggcca tcatttgtgg gtttgaaaaa
      781 attattcggg atgcagtgtt gatatagggt agcatttttt gcttctggct gtcagtcctt
      841 ttgtaaaaat cgctacccca gtaggagata atgaggtttt gtgtgagatt ggtaaagttt
      901 gaaatgttga tgtagaaaaa gtttttgttg aaaaaatgaa ggtgaatgta atcgtatttt
      961 ccccctgttt gttttaacga gcgcctaatg acgaacctca tcaggaaaaa ggatagatag
     1021 ggatttactt tatcagttgt tttttgaata ttaagtagca atttacatgg gagcagctct
     1081 ttgtggaaaa tattcctatc aatagccaga tggctagggt tagtatttaa aatatcaact
     1141 accaggttac tatttctttc gcaaatattg ctgacaatgt cgtgaagtag aaatgaattt
     1201 gcatctccaa caatcagaat gttttttttt accattttca atactattta gtcccaatgt
     1261 acttggcaaa tttcctaaat gttattttgc aggtagttgt gccaatcacc tatgtcaaac
     1321 catgattttt cagataccgg gaaaacgcct atacgaccac tgttggtcag gatactgtca
     1381 attaggtggg tcatgtggaa atacttgttg gtgggaataa ggtttaacac ccttgaatgg
     1441 aggatgtaca ttcccgagtt aatctggaag gtaaattctg gtttttcggt caatctttta
     1501 agcataccat ccgtttccgt ttccagtatg ccgtagggaa tagagtaatg ttttaaggct
     1561 gatacaatag ttaaatcgtt atggttggac tggtggtagt caagaatttc agagtaatcg
     1621 ttacggatta gtatgtcgca gttagataca aaaaaatctc cgctgattac acctttcagt
     1681 gagtaaagtg caccagccgt acctaaaggt ttttcttcgg ttaggtagga aatgttgaac
     1741 cgttcgccga atttttcgtc aacaaactgc cggagcatat tggctttata gtttacagag
     1801 atgtagaact ggctggttcc aaaacctgta aagctattta tgattctttc aatcatgctc
     1861 tcgcttccaa tagggataag cgctttgggg aggacatagg ttagtggcct taaacgtgtt
     1921 ccttctcctc cggccataat tactacagga tgattaaagc tatgcttgat aggcttttcc
     1981 ttttcagcaa aaacatcttc ccaaaaaata acatctacaa gggattgtga ctcatctaca
     2041 atgggcatgc actcaatacg ttgatccagc atttttctgc taatatcctc gctggagtcc
     2101 aaagtagtgg agattaaggt ctccttgcgt agaattgacg atacgggtgc attcaagggt
     2161 ttgtttttaa tgatagccct ttgaatatct ccaatactga gaatgttgat aaacctgtta
     2221 ccctcaaaaa ccagaagtag cctcctgtac gtggagtcca ttattttaag cgcatcaaca
     2281 atagaggcgt tttgttgaat tttaatggct tcgatttttt cctgtttggt tgccatctct
     2341 tcatgctatt ttaccagtgt gtttgcctta atgaagctgc caaccggtat ggttactcct
     2401 tgtcggaggg ttgcattact cccgataaaa ctttgctcgc caacagtaac ttcaccgttt
     2461 agtattgctc ctgttgatat atggcagaaa tcctcaactc ttgtatcatg ctcaactagt
     2521 gctttggagt tgattatgca gtttttacca ataacagccc ctgcatttac cagaacatgg
     2581 tgcatgataa ttgttccctc gccaatttcg gaatggggtg agatgtatgc taggggagag
     2641 atgattacgg gtaagcttac gttgagctgt tttaacaggc gaaaaagttt gagtctgggc
     2701 tggggcgact cgatttggcc aacagtaatc agaaagtgct tgtaattggt agccaagctg
     2761 gggagttcat catcggtaaa acttataggg taccctaaaa cattttgccc aacacgggat
     2821 gggatatcaa caatagcagc aatttcgtat aatccttgtt gctcaataac gtcaattgaa
     2881 gctctgcatt gaccgcctcc tccaaccagt attatttgtt ccttttggct catatcctta
     2941 cgctgcttgg aatgttaaca actctatcct ctagccactg ggcattggta agatcatcag
     3001 tctgctcgtt tttgaacatt ggcagcttgt tcataagcgt ccacattggt cgggtggcaa
     3061 tgcctttatc gtttgagtag gctagaaatg agtccctctc tgccctgtcc gacaacacta
     3121 tggcattaag ccagtagttt gatttagagt gaaccggttc ggtgaaaaag tttacgccaa
     3181 ttgactcgaa gtaggcctta tacctaagtg ccgtttccct tttattttta agaaagattt
     3241 caatttgctc catttgtgcc acacccaacg ctgcattaat gttgggcatt cggtagttgt
     3301 atccaacttc atcatgataa aactcccatg gatgtggaac ctttgcctgg gtggtcaggt
     3361 gcttaagttt tttacctagt tcaacatcat cagtaagaat cattccgccg cccccggtag
     3421 ttacaatctt gttgccgtta aaactcagaa cgcccatttt gctaaatgtt ccggtatgcc
     3481 tacccttgta gaagctaccc agactttcag cagcatcctc aacaacagga atattatgct
     3541 gattgcagat ttcaacaata tcttcaattc ggcagggatg gccaaatgtg tgcatgggaa
     3601 ctacagcaga tatttttctg ccagtatgct tattatataa tccatagcta ttgctattct
     3661 gattttttcg tgtttcagta ttcaatttta accaggaatc gagcttttcg ggcgacaacc
     3721 ctaaggtatc tctatccaca tcaataaaaa caggatgtgc gttgcagtag gcaatagcgt
     3781 tggctgtggc aataaaggta accggctggg ttataacttc atcatcatgc ttaaccccag
     3841 cggcaaggag tgctaagtgt aaggcgttgg ttccattaac cgtaacaact gctttcttag
     3901 atcccgtata ggtggcaaca acctcctcaa acttatcaac atactggcct acgcttgata
     3961 caaaggtaga atctatgcag gaaagcaagt aatccttttc tttgccttta aatacggggg
     4021 aatgtagcgg tataataccc gtaccgggat aagtgctatg aataaaatct acaatttcat
     4081 caaacttagg ctgattcatt tttatattta attagacgtg caggaactcc accaacaatc
     4141 gagttggcct caacatcatt aattactaaa ctattggccc caatgattga attatcacca
     4201 atggtgactc cttcgcgtat aaatacgtta gtgcctaccc acacgtggtt gccaattctg
     4261 atatctttct caatcgtagg ccttttgttc attggcccgg tactttcctc tgtatcatgt
     4321 gtccatgcaa tgatgttcac attatgccct atggcacacc attctccgat taccactttt
     4381 gatttaggtc cggttactat tcgcccgcca ttgaagtagg tgttttttcc aatgtgaatg
     4441 ttaccatgaa accaggtatt ttctaggtag ggtagcctga ctgttgggtc aatatcaaac
     4501 tttttcaggt actcccttcg tttttcttta aggtgatgct ttaatagtaa atggtaaata
     4561 cttgccagca gcttcatttg tagaaattct tttatgagtt ggaatacttg cgagtcatcg
     4621 tacaaaattc caacttaaac aattgttata gctacgttat tgcgtaaagt tcaaaattat
     4681 aatccttcac atttcaagtt atacgttttt aaactcggca ttatcagggt ttaaaagaat
     4741 tctctgtttt attactttca gaatctgcga aataccatcg ggtacacgtt gtgtaatact
     4801 aaaacccagt tcatttttta ttttttcaaa tgatacccgg taatcgcgag ggtcctcatt
     4861 tttctggata tacttaattt tggagtccgg aataaatttc ttaatttcct caacaatcat
     4921 ttttttctgg tagttttcgc ttgaatcacc aacattatat acgttgaacg caaccttttc
     4981 cattggggct tcgagtgcta tgagtactga tcgggccagg tccactacgt ggcaatatgg
     5041 cctccagaac tgctcgccga aaataaccag ctcccttccc agggccaact cctttgtaaa
     5101 ctcatttact gtcaggtcaa atcgtggccg taaggataac ccgtaaacag tggagaatcg
     5161 aagaacagtt ggtttacacg tgttggattt gggttgcgat agtaaaaact tttctactcc
     5221 aactttggtt tcggcgtata gtgaaactgg tgcaagttcc gacatctcgt caaccataat
     5281 atcggggttg gccattctac cgtaattact gcaggttgag gaaaaaataa aacgttttac
     5341 ccccatttta ttggcaattg agtagagtag cttgctccca tcgaggttag tgcttttggc
     5401 taattcgggt tgtttggcac aggcgggatc gccaactata gcagcaaggt gcacaacaaa
     5461 atctacccct ttcatagcca tggcaatatc ttcttcacgt cgaatatcgc cttttacaaa
     5521 atcaaaatta ttgttgttga ataaatcaag cagagattct cctccgtaca ttaagctatc
     5581 caaaacaacc accctataat tatgctccag aagttgacgc accaaaactg aaccgatgta
     5641 tcccgctcca cctgttacta gtactttttg cattgactaa ttttgttaat gatgtaataa
     5701 agttttgatt tttctttatg cacaggagaa gttaatgcct atgctatccg taaacctcct
     5761 tcctggctgc cttgagggta tttttgagca acgaggcaa
//
LOCUS       DAOSHM010000104         5701 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_12480_length_5701_cov_21.070138, whole genome shotgun
            sequence.
ACCESSION   DAOSHM010000104 DAOSHM010000000
VERSION     DAOSHM010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 5701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5701
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12480_length_5701_cov_21.070138"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(28..2487)
                     /gene="pheT"
                     /locus_tag="PK839_10170"
     CDS             complement(28..2487)
                     /gene="pheT"
                     /locus_tag="PK839_10170"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOG73140.1"
                     /translation="MKISYSWLKQYVDTDLAPERVSEVITSTGLEVDSMERIEAIRGG
                     LAGVVVGEVLTCDKHPDADKLSVTTVDVGEQKPLSIVCGAPNVAKGQKVLVATIGTTL
                     YFSGGEVTIKKAKLRGVSSEGMICAEDELGLGTSHEGIMVLPGDTPVGMPARELFKLD
                     DDYVYEIGLTPNRIDAASHYGVARDLAAYLAFHSNTDTKAVKPDVSGFSIDSKSLTIP
                     VEVVNTSACRRYSAITVSNVTVAPSPEWLQKRLKSIGINPINNIVDVTNYVLHEVGQP
                     LHAFDADAITGGRVVVQTLAEGTPFITLDGVERKLSADDLMICNGSEPMCIAGVFGGL
                     SSGITAKTTRVFIESACFNPVYVRRTARKHGLSTDASFRYERGTDPNITIWALKRAAM
                     LIREVAGGQISSDIVDVYPEPLDHFRVEFNVDSACRLIGMRIPNDQIERILTSLEISV
                     IEKNDYHWVLAVPTYRVDVTREADVVEEILRIFGYDNVEIPEKVSSTLNFSKKPDNFT
                     MQSIACGFLTGNGFNEIMCNSLTKKDYYEKLTTYPARSSVEILNPLSSDLNVMRQTLL
                     FGGMESIQRNTNYRRPNLKLFEFGNCYRYNADLADVTNPLDRYSESFRIAMWMTGLME
                     PEHWMDKHNSVNFYHLKAFVHALLARFGLDVDTLSHTEAPSDLFSYGLSYLSGSAPVA
                     SFGLISSSIADSFDIKAEVFFAEIEWSALLTKLGETKISFRELPKYPEVRRDLALLLD
                     NSVSYEQVRKLAFATERKLLRQVTLFDVYQGNNLPQGKKSYAVSFILQDETRTLTDEQ
                     IDATMKRLVDAYAKQLGAQLR"
     gene            3073..4431
                     /locus_tag="PK839_10175"
     CDS             3073..4431
                     /locus_tag="PK839_10175"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008150997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HOG73141.1"
                     /translation="MKILKFGGTSVGDAQRIRKVAQLLPEAEPCIVVLSAMAGTTNAL
                     VEIANHSSSGNVSMAIEQAKQLEARYMETSHELFNSPVFRNRGEKFTNDMFAELYRKL
                     GCPPTKKVALEIVALGEQLSTGLFHLYLTENRIRSTYLPALEFMRIDREREPDYFYIS
                     QSLERLIKANSFTRVFITEGFICRNAKGQTDNLGRGGSDYTAAIIGNVADSEEVQIWT
                     DIDGLRNNDPRHVVNTKPIRHLSYGEAAELAYFGAKILHPSTIQPCSEKNIPVVLKNT
                     LQPDDPGTLIDSVYIPKGIKAIAAKKGITAITIQSSRMLLAYGFLSHVFEVFNRYKTP
                     VDMIATSEVSISLTIDDCSNLQGIANELAEFSTVECTYDQTIVCIVGDFGANRKGYAA
                     RIFNALHEIPIRMISYGGSSNSISILVDSQSMTRTLQLLQEVVDTDVDVNLEQTANTI
                     SY"
     gene            4438..5598
                     /gene="lysA"
                     /locus_tag="PK839_10180"
     CDS             4438..5598
                     /gene="lysA"
                     /locus_tag="PK839_10180"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HOG73142.1"
                     /translation="MKTPYYQYDLQLLRQTLDAATREANKYGYTLHYAVKANANPVIL
                     NEISSYGLGADCVSGNEISAALSGGFNPKGIVFAGVGKTDEEIELAIARGIYCIHCES
                     VQELMTIQKIASAMQTTANVALRVNPNVDAHTHKHITTGTMANKFGLTVEEARRIIKN
                     FDRYSNLNLFGLHFHIGSQITEMNVFEELAQRVNELQHDFYANGKPAYLNLGGGLGIN
                     YQNPDSELIPPFMNYFDVFARNLNRNSWQSIHFELGRSLVGQCGKLVTRVLYTKKMGS
                     KNFAIVDAGMNALIRPALYGAYHRIVNTSTSSSKMERYEVVGPLCESTDILGTNVLMP
                     VTQRNDVLEVLSCGAYAEAMSSRINLRDIPDYCFTNQLQEKTITKTGAPLQG"
BASE COUNT         1622 a         1394 c         1256 g         1429 t
ORIGIN      
        1 tttatccttt atcctttttg ttaacggtta cctcagctgc gcacccagct gtttggcata
       61 ggcatcaact agccgcttca tggtagcatc aatctgttcg tcggtaagcg tcctagtctc
      121 gtcctgtagt ataaagctaa cggcataaga ttttttaccc tgtggcaggt tgttgccctg
      181 gtaaacatca aaaagtgtaa cctgccgcag cagcttacgc tcggtggcaa aagccaactt
      241 tcttacctgt tcgtagctaa cgctgttgtc gagaagcaag gccaaatcgc ggcgtacctc
      301 aggatacttt ggtaactcgc gaaagcttat ttttgtttca ccaagcttcg taagaagtgc
      361 actccactct atttcagcaa agaaaacttc tgccttaatg tcgaagctat cggcaatgga
      421 gctgcttata agcccaaaag aagccacagg ggcgcttccg cttaagtagc ttaagccata
      481 gctaaaaaga tcgctaggag cttcggtatg gctaagggta tcgacatcaa gcccaaaacg
      541 tgcaagcaga gcgtgaacaa aagcttttag atgataaaag ttaacggaat tgtgcttatc
      601 catccagtgt tcgggttcca ttaagccggt catccacatt gcaattctga acgattcgct
      661 gtaccggtcg agcggattgg taacatccgc caaatcggca ttataccgat agcaattccc
      721 aaattcaaac aacttaaggt tgggcctcct gtagttggtg ttccgctgaa tgctctccat
      781 tcccccaaat aggagggttt gccgcatcac gttcaagtcg ctgctcagcg ggtttagtat
      841 ttcaaccgag ctacgggcgg gataggtagt caatttttcg tagtaatcct ttttggttag
      901 tgagttacac ataatttcgt tgaaaccatt tccggtaagg aagccgcagg caatgctctg
      961 catggtaaag ttatcgggct ttttggagaa attcagagtg ctgctgacct tttcgggaat
     1021 ttccacatta tcgtacccga aaattctgag aatttcctcc acaacatcgg cctccctggt
     1081 aacatcaacc ctataggtag gcacggccaa cacccagtgg taatcgtttt tttcgatgac
     1141 tgaaatttca agagaggtca gaatcctttc aatctggtca ttgggtatcc tcatcccaat
     1201 aagccgacag gcactatcaa cgttgaactc taccctaaaa tgatctaaag gttcgggata
     1261 cacgtcaact atatcggatg aaatttggcc accagctacc tctctgatga gcatggcggc
     1321 acgctttagc gcccaaatgg taatgttggg gtctgttcca cgttcgtatc gaaacgaggc
     1381 atcggtgcta agtccgtgtt tgcgggctgt tcgccttacg taaaccgggt taaaacatgc
     1441 gctctcaata aacaccctgg tggttttagc cgtaattccc gaagacagcc caccaaacac
     1501 accggcaatg cacatgggtt cggagccatt gcaaatcatg aggtcgtcgg cagaaagttt
     1561 acgttcaacc ccatcgaggg tgatgaatgg ggttccctct gccagcgttt gtacaaccac
     1621 ccttcctccg gtaatggcat cggcatcgaa cgcatgcagc ggttgtccca cctcgtggag
     1681 cacatagttg gtaacatcaa caatgttgtt gatgggattt atgcctatgg actttagcct
     1741 tttctggagc cattcgggcg atggcgcaac agtaacatta cttactgtaa tggccgagta
     1801 gcgacggcag gccgatgtat tcaccacctc aacagggata gtcaaacttt tgctatcaat
     1861 gctaaaacca gacacgtctg gcttaacagc cttagtatcg gtattgctat gaaaagccag
     1921 gtatgcagcc aaatctcggg ctacaccata gtgcgatgca gcatcaatgc gatttggtgt
     1981 aagcccaatt tcgtaaacgt aatcatcgtc gagcttaaat agctcacgag ccggcatccc
     2041 aacaggggta tctccgggca aaaccattat cccttcgtgc gaagttccaa gtcccaattc
     2101 atcctcggca caaatcatcc cctcggagct aacaccacgt agcttcgcct ttttaatggt
     2161 aacctcacct ccgctaaagt aaagcgtcgt gccaatggtt gccaccagta ccttctgacc
     2221 ttttgccaca ttaggtgccc cgcaaactat gctaaggggc ttttgttctc caacatcaac
     2281 tgttgtaacg ctaagcttgt cggcatcggg atgcttatcg caagtcagca cctcccccac
     2341 cactactccg gcaaggccac ccctaatagc ttctatgcgc tccatgctat ctacctccaa
     2401 tccagtagag gtgattactt cagaaacacg ctccggtgcc agatcagtat caacgtactg
     2461 tttaagccag ctgtaggaaa ttttcactgt gatataatta gttaagcact tgaatttatg
     2521 tgatatacat tcagaattaa atcaacctcc ttaactacgg aggcatcagt accataccat
     2581 ttttctgtta gcaaaatggg ttgtcggcat ttgtcagcaa aaaaatggct acaccgaccc
     2641 accaataacg ttgcaaattt taccaaggaa gcatttgccg gcaaaagcat tataaaaaat
     2701 ccgccatatc ctgctaaccc tttgtggtag caaaagtaag cattattgaa aaactcccca
     2761 aaatgccatc ccattcatca gaaaaaaaat ctacagggaa aactttatta gaaattatat
     2821 acatttacgg cggaaaatga aaccgaattt tccacgtcac catgaataaa acgacccgca
     2881 atagcataaa aatgaacatg atgctccata aaggcatgat gcgtaaatct atgagcataa
     2941 aacgagtgcc gttgcaggtc ttactctcgc atgtaaagta cactttgtgg taatatacta
     3001 aaaactaagc gattaaaagg cctgcaaaca atgcaggcct ttttttattt tttaaatcct
     3061 aaaagcacag aaatgaaaat actcaaattt ggtggaacat cggttgggga tgcccaacga
     3121 ataaggaaag tagcgcagct gctacctgaa gcagaaccct gcatcgtggt gctatcggcc
     3181 atggcgggaa ccacgaatgc cttggttgag atagctaacc atagtagtag tggaaatgtt
     3241 agcatggcga tagaacaggc caaacagctc gaagcaagat acatggaaac atctcacgag
     3301 cttttcaatt cgccggtttt caggaataga ggagaaaagt ttactaatga tatgtttgcc
     3361 gagctttaca gaaagcttgg ttgtccgcct acaaaaaaag ttgcgcttga aatagttgcc
     3421 ctaggcgagc agctttccac gggtttgttc cacctctacc tcacagaaaa cagaatccgc
     3481 tcaacctacc ttcctgcttt agagtttatg aggattgaca gggagagaga acccgactac
     3541 ttctacattt cccagagcct ggaacgatta atcaaagcca attcctttac ccgggtattc
     3601 attaccgaag gatttatctg taggaatgcc aagggccaaa ccgataacct gggtagaggt
     3661 ggaagcgact ataccgccgc aataatagga aatgtagctg attcggaaga ggtgcagatt
     3721 tggacagaca ttgatgggct tcgcaacaat gacccccgcc acgtggttaa caccaaacca
     3781 attaggcacc tatcgtacgg agaagctgcc gagctggcct acttcggtgc gaaaatacta
     3841 cacccgtcaa ccatccaacc ctgcagcgaa aagaatatcc ccgtggtgct gaagaatacc
     3901 cttcagcccg acgaccccgg aacccttatc gactcggtct atataccaaa aggaattaag
     3961 gccatagctg ctaaaaaagg cataaccgcc attaccatcc aatcgtcgag aatgctgctg
     4021 gcatacgggt ttcttagcca tgtattcgag gtgttcaacc gttacaaaac tcctgttgac
     4081 atgattgcca cctctgaggt ttctatctcg ctaaccattg acgattgctc caacctacag
     4141 ggtattgcta acgagctggc agaattttca acggttgaat gtacctatga ccaaactata
     4201 gtatgcattg ttggcgattt tggcgccaac cgcaagggct atgcagcccg aatcttcaat
     4261 gcactgcacg aaattcccat caggatgata tcatacggag gaagtagcaa cagcatttcg
     4321 attctggttg attcccaatc aatgaccaga accctgcagc tgctgcagga ggttgttgat
     4381 acagatgtag atgttaacct tgaacagacg gcgaatacaa tttcatacta gcacaccatg
     4441 aagacacctt actaccagta cgacctgcag cttttaagac aaacactcga tgcagcaaca
     4501 agagaagcta acaaatatgg ctacacccta cactatgcgg tgaaggcaaa tgccaatccg
     4561 gtaatactca acgaaatatc atcgtacgga ctcggtgccg attgcgtgag cggtaatgag
     4621 atttccgcgg cactatcggg tgggtttaac cctaaaggca ttgtgtttgc cggtgtaggt
     4681 aaaaccgatg aggaaattga gctggccata gccaggggca tatactgcat acactgcgaa
     4741 tcggttcagg agctaatgac cattcaaaaa attgcaagcg ccatgcaaac aaccgccaac
     4801 gtggccctta gggtaaatcc caacgttgat gcccacaccc acaagcatat cactaccgga
     4861 accatggcca ataagtttgg acttacggtg gaagaggcca ggcgaattat aaaaaatttt
     4921 gaccgctaca gcaaccttaa cctctttggg cttcactttc atattggttc gcaaatcacc
     4981 gaaatgaacg tgtttgagga gttggcacaa agagtaaatg agcttcaaca cgacttctac
     5041 gccaatggta agcctgccta cctcaacctg ggcggagggt tgggaataaa ctaccagaat
     5101 cctgattcag agcttatccc tccatttatg aattactttg atgtttttgc acggaacctt
     5161 aacaggaact cctggcaaag cattcacttc gagctggggc gctcgttggt gggccagtgc
     5221 ggcaagcttg taaccagggt tctctacaca aaaaaaatgg gtagtaagaa ttttgccatt
     5281 gtggatgctg gaatgaacgc ccttatccgc cctgcgcttt acggcgccta ccacagaata
     5341 gtgaacacca gcaccagcag ctccaaaatg gaacgctacg aggtggttgg cccgttgtgt
     5401 gagtcaaccg atattcttgg aactaacgtt ttgatgcctg taacccagcg aaacgacgtg
     5461 ctggaggtac tttcgtgcgg cgcctatgcc gaggccatga gctcacgcat caaccttagg
     5521 gatattcccg attactgctt taccaaccag ctgcaggaaa aaacaattac aaaaacagga
     5581 gctcctctgc agggctgata tcaactcaaa ttagctaccg ttgttgccta tattctcaaa
     5641 acccatgtta gtagctattc tataaggtga atgttgctgt atgttcagta gaatgaaccg
     5701 a
//
LOCUS       DAOSHM010000105         5573 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_12904_length_5573_cov_22.212577, whole genome shotgun
            sequence.
ACCESSION   DAOSHM010000105 DAOSHM010000000
VERSION     DAOSHM010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 5573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5573
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12904_length_5573_cov_22.212577"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..654
                     /locus_tag="PK839_10185"
     CDS             <1..654
                     /locus_tag="PK839_10185"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOG73143.1"
                     /translation="AGSSLEPVHRARAAGQKVYAETCPQYLLLNSSCYTDDFWPAAKY
                     VISPPLRSPSDQEQLWKGIQDEDIQTVGTDHCPFSSSQKAYGINDFRKIPNGAGGIEH
                     RLALLYTYGVLEGNISINRMVDLFSTQPAKIFGLYPQKGEIAVGSDADLVIWNPAPTS
                     TIQASNHHSQADFNIYEGMKVKGEAKYVFKKGYLAIADGALQQNLPKGKLLRRNTRI"
     gene            947..2371
                     /locus_tag="PK839_10190"
     CDS             947..2371
                     /locus_tag="PK839_10190"
                     /inference="COORDINATES: protein motif:HMM:NF015557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HOG73144.1"
                     /translation="MNFKMLLKNVLSIKNGKPLAFLLMLLFLPVFVFASEGGSEAPSI
                     GGIRVEFILFGLTLIGVALFHKQTFWVAICGLTAIVLYKLLFISGYPFAEHFFGENPM
                     VDQILHKDLRQGEWGILVNLLGLLLGFAILAKIFEESGVPDILPRFLPSGWQGPFVLL
                     IFIFILSSFLDNIAAAMIGGTIALVVFKNKVHVGYIAAIVAASNAGGAGSVVGDTTTT
                     MMWIDGVSALNVLHAYVAAGVALIILAWFASHQQDHHQQIVKDAKTDVKIDWVRLGIV
                     VLILAGAILSNIYYDMPALGVWIAILIGAIFTKIPWQEVGASIKGTIFLLCLVTSASF
                     MPVETLPDASWMTAFILGFVSAVFDNIPLTKLCLDQGHYDWGMLAYAVGFGGSMIWFG
                     SSAGVAITNKFQEAKNVTLWLRKGWHVTLAYIVGFFTLYITMGWEPASNKQHQEPAIE
                     CKAPDCPVRAKAEFERTLHPDEVK"
     gene            complement(2666..3856)
                     /gene="ygeW"
                     /locus_tag="PK839_10195"
     CDS             complement(2666..3856)
                     /gene="ygeW"
                     /locus_tag="PK839_10195"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF005538.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HOG73145.1"
                     /translation="MDRVEIIERLKTNLHGRDFLLTWEKSEDELRMILGVADILKEMR
                     NRNISTRVFDSGLAISNFRDKSTRTRFSFASASNMLGLAVQDLDEEKSQISHGETVRE
                     TANMISFLSDFIGIRDDMYLGEGNKYMRQVGTALDDGFANGVLPVRPGIVNLQCDMDH
                     PTQVMADLLHLTHEFGSLEALRGKKLVMSWAYSPSYGKPLSVPQGVIALMTRFGLNVE
                     LAYPEGYDLIPEIVDVARNNAAKSGGSFTIKHSMAEAMRGADIVYPKSWAPFHIMEQR
                     TQLLKSGDKKLLNELEQTCLANNARHKDWEYNEEMRRLTSKSDALYLHCLPADISGES
                     CAQGEVSAETFERYRIKTYLEAGYKPYIIAAMMLANRFEDTAGLMKKLLDRKQERVTS
                     VLKL"
     gene            complement(4008..5195)
                     /locus_tag="PK839_10200"
     CDS             complement(4008..5195)
                     /locus_tag="PK839_10200"
                     /inference="COORDINATES: protein motif:HMM:TIGR03526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HOG73146.1"
                     /translation="MSEISLKVKELSEKYRDYTASNLSRLVRIKSLSTQEHDVQLELK
                     RQMEEAGFDEVRIDGLGNVVGRIGNGSRILAIDGHMDTVDIGYRNNWDFDPLGGEIRD
                     GFVHGRGSVDQKGGPASFVTSGRILKELGFDRDLTIYFVGSVMEEDCDGLCWKYIIEE
                     DGIKPDFVISTEPTNLNIYRGHRGRMEMHVQFYGVSAHGSAPERGKNAIYMASRAALE
                     IEKLNEHLKVDEFLGKGSVTISEIVSGSPSLCAVADYARIHLDRRLTWGETMETAVGE
                     VEEIVKNMNAKVEVLNYSEKAYTGLEYGMVKYYPTWKMPEDHLIVQTGVKAFSQLYGK
                     NPKVDKWTFSTNGIMTCGVYGIPTIGFGPGNEVLAHAPNEKVPVNDLVVASAFYALFA
                     YTI"
BASE COUNT         1446 a         1330 c         1226 g         1571 t
ORIGIN      
        1 gcgggcagct ctcttgagcc tgttcatcgt gcaagagctg ccggacagaa ggtttatgcc
       61 gaaacctgcc cgcagtacct tctgctcaac agctcctgct acaccgacga tttctggcca
      121 gctgcaaagt atgttattag cccgccgctc cgctcaccga gcgatcagga acagctttgg
      181 aaaggcattc aggatgaaga catccagacc gtgggaaccg accattgccc cttcagttct
      241 agtcaaaagg cctatggaat aaacgatttc cgaaaaatac ccaatggcgc agggggaatt
      301 gaacatcgtt tagctttgct ctatacgtat ggcgttttgg aggggaacat tagcatcaac
      361 cggatggttg acctcttttc aacccaaccg gcaaaaatat tcggacttta tccccaaaag
      421 ggcgaaatag ctgttggctc cgatgccgac ctggttattt ggaatccggc ccccacctca
      481 accattcagg caagcaacca tcattcgcag gcagacttca acatatatga aggcatgaag
      541 gtgaaaggtg aggcaaagta cgtttttaag aagggctacc tggctattgc cgatggtgca
      601 cttcaacaaa atctaccaaa aggaaagcta ttaaggagaa acacccggat ataaataagt
      661 ataaaaaatt gtattttggg ctaccgggta atacactaag gagaatacat tatttttttc
      721 catttcaacc acctctacgc cgaaggtgga gatattttgt cccagctgat tttgcttgtg
      781 tgaagaccat actgttagat attcccatgt attttgacct ctaaaatgcc ttaaaaaaac
      841 aacatttgct aaaattatct attaccaacc gcaaaagcta gattattttc attgatttgt
      901 taaatttgac gcattcctaa cctaaaatta actttaaact aaacttatga atttcaagat
      961 gttactaaag aatgtcttga gcataaagaa cggtaaaccg ttggcatttc tgctcatgct
     1021 gctgtttttg cctgtttttg ttttcgcctc cgagggagga agtgaggcac cttctattgg
     1081 tggaatccgg gttgaattta tactgtttgg cctaaccctt ataggagttg ccctttttca
     1141 taaacaaacc ttttgggtgg caatatgcgg attaacggca attgtgctct acaagctgct
     1201 atttatttcc gggtatcctt ttgccgagca ctttttcgga gaaaatccga tggttgacca
     1261 gatattgcac aaggacctgc gtcaggggga atggggtatt ctggttaacc tgctgggatt
     1321 gttgctggga tttgccatac tggcaaaaat ttttgaggaa tcgggcgtgc cggatatact
     1381 acccaggttt cttccaagtg gttggcaggg accttttgta ctgcttattt tcatcttcat
     1441 cctctcgtca ttcctcgata acattgccgc tgcaatgatt ggtggcacaa ttgccctggt
     1501 agttttcaag aataaagtac atgttggcta tatagcggca attgttgctg cttcaaatgc
     1561 aggaggtgca ggtagcgtgg ttggcgacac caccaccacc atgatgtgga ttgacggggt
     1621 ttcagcactt aatgtactac atgcctacgt agctgccggg gttgccctta taattctggc
     1681 atggtttgct tcacaccagc aggaccacca tcagcaaatt gttaaggatg caaagactga
     1741 tgtaaagatt gactgggttc gcctgggaat agtggtgctt attcttgccg gtgccattct
     1801 ctcgaacata tactacgata tgcctgccct tggggtgtgg attgccattc ttattggggc
     1861 aatttttacc aaaattccct ggcaggaggt tggagcatcc attaagggaa ccatttttct
     1921 attgtgcctg gtaacctccg cctcgttcat gccggttgag acccttcccg atgcttcatg
     1981 gatgaccgca tttattctgg ggtttgtttc ggctgttttt gacaacatcc cgctaacaaa
     2041 gctctgcctt gaccaggggc attacgattg gggcatgttg gcctacgccg tgggctttgg
     2101 aggttctatg atttggtttg gttcgtcggc tggcgtggct ataaccaata agttccagga
     2161 ggctaaaaat gtaacgctat ggttacgaaa aggctggcac gttaccctgg cctatattgt
     2221 cggtttcttt accctataca tcaccatggg atgggaacct gccagcaaca agcagcacca
     2281 ggaacctgca attgagtgta aggcacccga ctgtccggtt agggcaaaag ctgaatttga
     2341 gagaacattg caccccgatg aggttaaatg atctgtaact ccggaataga gaaaacactt
     2401 tgtgctttag ctattaaatt ttataagtga agaaggtgct accagctaga aattttagct
     2461 aaattaaaaa acagaggaat tgctgaaata aaagtcaacc cttttggttg attttcctaa
     2521 aagcgaaaaa acctgccccc cacatagatg gttccggcag gttttttcgc tatttcctta
     2581 gtaagctata tgggcagaaa aaattatggt ttgcccaacc cggctagaat tgttcttacc
     2641 ctggcgcaaa gctccaacta ccttcttaca gctttaaaac actggtaacc cgttcctgct
     2701 ttcggtctag taattttttc attaatcctg ctgtatcctc aaagcggtta gcaagcatca
     2761 tggctgcaat tatgtagggc ttatatcccg cctcaaggta ggtctttatg cggtagcgct
     2821 caaaagtttc agcacttacc tcaccttgtg cacacgattc accggaaatg tcggcgggca
     2881 ggcagtggag gtaaagggcg tcggacttac tggtcaacct acgcatctcc tcgttatact
     2941 cccagtcctt atgccgggca ttgttagcca ggcaggtttg ctccagctcg ttcagtaact
     3001 tcttatcgcc actttttagc aattgggttc gctgctccat gatgtgaaat ggagcccagc
     3061 tttttggata aactatatca gcgccgcgca tggcttcggc catggaatgt ttaatggtaa
     3121 acgatccgcc gcttttggca gcattattcc tagctacatc aacaatctcg ggtattaagt
     3181 cgtaaccttc cgggtatgcg agctccacat tcaagccgaa acgggtcata agggcaataa
     3241 ccccctgggg gaccgataga ggttttccgt agctagggga gtaagcccaa ctcatcacca
     3301 gctttttacc cctcaaggct tccagggagc cgaattcatg ggtaaggtga agcaaatcgg
     3361 ccataacctg ggttgggtgg tccatatcgc attgcaggtt gacaataccc ggcctcaccg
     3421 gtagcacgcc gttggcaaac ccatcgtcga gtgctgtgcc aacctgccgc atgtacttgt
     3481 taccttcgcc aaggtacata tcgtcgcgaa tgcctataaa atcggataga aacgaaatca
     3541 tgttggcagt ttcacgaacg gtttccccat gcgaaatctg cgatttctcc tcgtcaagat
     3601 cctgaacagc cagccccagc atgttggagg ccgatgcaaa agaaaatcgg gtacgggttg
     3661 acttgtccct aaaattggaa attgccaggc ccgagtcgaa tacccttgta gatatattac
     3721 ggtttctcat ctccttaagg atatcggcta ccccaagtat catccttaac tcgtcttcac
     3781 ttttctccca cgttaggagg aaatccctgc catgcaggtt ggtttttaac ctttcgatga
     3841 tttcaactct atccatcaac ataaattgtt taaataaaca gacaactttt taactcgatt
     3901 agcaatagct ataggcagct tccattaaaa ttcctctaaa caaaaataac agcagcatca
     3961 ctgccctaaa atggtaaaga gaaagtaaac ctaaaaattt caaccagcta gatggtgtag
     4021 gcaaataggg cgtaaaatgc tgaggcaacc accaaatcgt taacaggcac cttttcgttt
     4081 ggagcatgag ccagcacctc gttgcccggg ccaaatccta tggttggaat tccgtaaacg
     4141 ccacaggtca taataccgtt ggttgaaaat gtccacttgt caacctttgg gtttttgcca
     4201 taaagctggc tgaatgcttt aaccccggtt tgcacaatta aatgatcttc aggcattttc
     4261 caagttggat agtatttaac catgccgtac tccagaccgg tgtaggcctt ctcggagtag
     4321 ttcagcacct caaccttggc attcatgttt ttaacaatct cctccacctc gccaacagcc
     4381 gtttccatgg tttcacccca ggtcagcctg cggtcgagat gaattcgggc ataatcggct
     4441 accgcacaaa gcgacggact ccccgatacg atttcagaaa ttgtaacgct tcccttgccc
     4501 aaaaattcgt caaccttcag gtgttcgttg agcttctcaa tctccagagc agcccttgag
     4561 gccatgtaga tggcattttt gccacgctca ggagccgagc catgggctga caccccgtaa
     4621 aattggacgt gcatctccat tcttccccta tgccctctgt agatatttag gttagttggt
     4681 tcggtgctga ttacaaaatc gggtttaatc ccatcctcct cgatgatgta cttccagcat
     4741 agcccgtcgc aatcctcctc cattacgctg ccgacgaagt aaatggtgag atcgcggtcg
     4801 aagcccagtt ctttcagtat tcgtcctgaa gttacgaatg aggctggacc acctttctgg
     4861 tcaacagagc cccttccgtg cacaaaacca tcgcgaattt cacctcctaa cgggtcgaag
     4921 tcccagttgt tgcggtaacc aatgtcaacg gtatccatgt ggccatctat tgccaatatc
     4981 ctactaccgt tgcctattcg gccaactaca ttccctaacc catcaatcct tacctcatcg
     5041 aagccggctt cctccatttg tctctttagc tccagctgaa catcatgctc ctgggtgctt
     5101 agggatttta tcctgaccaa acgcgacaag ttactagccg tatagtcgcg gtatttctcg
     5161 gaaagttcct taaccttcaa gctaatctca ctcatgatga ttatcctatt ttggttcaaa
     5221 attaccggat atatcaatag ccacatcaag ttttagttgt aaattataca ttttattcaa
     5281 atttagtatt tttctcatag tattaactta cattataggt attttttcta tatataaagg
     5341 ctaactaaag gatggttaat atagctgtac catgaaaatg gttaaatatt aaaggaattc
     5401 agggcccgtc aaactctgac ggagcttgtg taactcttgg cggagaaaag ttaaatgtta
     5461 cgcggtcggg aaccgagccg ttttttggcg agttcgccga aggcaaaccg ctacttaaat
     5521 acgacgaagt tacgctttgc taaacttcgc cgagcggggg ttaacctttt ccc
//
LOCUS       DAOSHM010000106         5462 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_13223_length_5462_cov_4.947475, whole genome shotgun sequence.
ACCESSION   DAOSHM010000106 DAOSHM010000000
VERSION     DAOSHM010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 5462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5462
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13223_length_5462_cov_4.947475"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..882)
                     /gene="aroF"
                     /locus_tag="PK839_10205"
     CDS             complement(<1..882)
                     /gene="aroF"
                     /locus_tag="PK839_10205"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF006421.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HOG73147.1"
                     /translation="MLIQLNKNCSTSELNFILEAVKKMGYHPTPVKTFNRYYLVCMGN
                     AEVDIRIIGRLSGVSDVHIITDPNPLVSRQWRVANTEIDLDDGVVISRNNFYIAAGPC
                     AIEDENQVVKTVEFLKQHNIRLMRGGVFKPRSSPYAFRGLGIDALKFFSKHCHQNGIK
                     IVTEVMQVSQLEQMYPYVDIFQVGTRNMQNFNLLDALGKIDKPVLLKRGMSATLPEFL
                     SSAEYIFSSGNEKIILCERGIRTFEKEYRNTLDLNAIPYLKDKSHLPVFCDPSHGVGV
                     REYVEPMSLAAVMAGADG"
     gene            complement(893..1672)
                     /gene="trpA"
                     /locus_tag="PK839_10210"
     CDS             complement(893..1672)
                     /gene="trpA"
                     /locus_tag="PK839_10210"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011966081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HOG73148.1"
                     /translation="MSSLRELLQPGRKKILSIFITAGYPQLDSTNKLILALQDAGADL
                     VEVGIPYSDPLADGPVIQNTSSVALANGMSIAILFEQLQRIGRQVRIPLVLMGYLNPI
                     LQYGVEKFCLHASRVGISGLIIPDLPLEEYQASYQRTFQRYSLPFISLVCPSTSDERM
                     RLIDKLSDGFIYAVSTSSTTGKEAVFGSPQAEYLKHLKNLNLSNPILVGFGIHNAETF
                     EFATSFCDGAIIGSAFLKSIASCVDFEDVTSQFIRKVRVSS"
     gene            complement(1665..2873)
                     /gene="trpB"
                     /locus_tag="PK839_10215"
     CDS             complement(1665..2873)
                     /gene="trpB"
                     /locus_tag="PK839_10215"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018108589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HOG73149.1"
                     /translation="MTNHPDSRGYYGSYGGAYVPEMLYANIHELQQNYLNIIGSEDFR
                     KEFNELLTSYVGRPTPLYLARNLSLVKGARIYLKREDLCHTGAHKLNNVVGQMLIAKR
                     LGKNHIVAETGAGQHGVAAATMCALMKLKCTVFMGVKDIERQQPNVLRMKMLGATVIP
                     ATTGSQTLKDATNEAIRFWIDNPHNTHYLIGSTIGPHPYPDMVARFQSVISSEIRTQL
                     LEQEGVEKPSCVIACVGGGSNAAGAFYHFIQDKEVELFGVEAAGEGVNTFKTAATISK
                     GSEGIIHGCKTLLLQNDDGQILEAHSLSAGLDYPGVGPLHAYLNKTKRARYLAVTNEQ
                     ALNASFELAQAEGIIPALESAHALAALSMLSFKPNDIVVVNLSGRGDKDMETYSENYR
                     KHTPNSLTYE"
     gene            complement(2870..3490)
                     /locus_tag="PK839_10220"
     CDS             complement(2870..3490)
                     /locus_tag="PK839_10220"
                     /inference="COORDINATES: protein motif:HMM:NF012900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HOG73150.1"
                     /translation="MKLKICGITEKCDIPAIVQLNPDYLGFIFHPASPRDISKRVAQI
                     SFNLIPKHIGRVAVFVDEPTRTVISLVEKYGFTHAQLHGSENPQQCREIQRYVPVIKA
                     FMINDALPLNLEQYQDCCAFFLFDTRGRHAGGNGRQFNHSLLSSYYLHTPFFLSGGIA
                     PGDASYLNRLSYPQLHAVDINSRFEDETGSKNIKLLNTFVNKLSRK"
     gene            complement(3509..4318)
                     /gene="trpC"
                     /locus_tag="PK839_10225"
     CDS             complement(3509..4318)
                     /gene="trpC"
                     /locus_tag="PK839_10225"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF001377.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HOG73151.1"
                     /translation="MNRLDEIVACKKREVAEKKELYPMKLLERSIYFNTPPLSLKTYI
                     MRPDLNGIIAEFKRKSPSKGTINEFAKPEQVCLHYMQAGASALSVLTDREFFGGTSDD
                     LIAARKFNYCPILRKDFVIDEYQVVESKSIGADAILLIAEILTKKELVALSAIASSLG
                     LEVLHEVHSRKSIDKLPPQASIVGVNNRNLNTFHVSINHAASLISDLPGDVAKVVESG
                     ITSPEMLLSMKGVGFNGFLIGEKFMREANPGKACQAFINRLNTLTLNGIVA"
     gene            complement(4322..5317)
                     /gene="trpD"
                     /locus_tag="PK839_10230"
     CDS             complement(4322..5317)
                     /gene="trpD"
                     /locus_tag="PK839_10230"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005675660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HOG73152.1"
                     /translation="MELKSILNKLLESQRLSHLEARDLMHGIAGGAVNEAQMSAVLTA
                     YVMRSISLDELIGFREALLELSQEVKLLNGEVIDLCGTGGDGKNTFNISTASTFVVAG
                     ANVPVAKHGNYSASSITGSSNIMEYLGYSFSSNPDKLKREIEQTNVCFLHAPLFHPAL
                     KAIGPVRRQLGVKTFFNMLGPLINPARPKYQLSGVFNAEVGRMFSYLFNRCLTDYRVV
                     YSLDGYDEISLTSDVKVFSKMGDECFSPESIGFSRLSPSCAAGGATVEESAGIFLSVL
                     GNQADKGKISIIIANSALAISCFKGISITEAAHQAQESIESGRAMEQLKRLLAMQ"
BASE COUNT         1437 a         1306 c         1198 g         1521 t
ORIGIN      
        1 cccatccgcc cctgccatca ctgctgccaa cgacataggc tctacatatt ccctaacccc
       61 taccccatgc gatggatcgc agaaaaccgg aaggtggctt ttatccttta agtagggtat
      121 agcgttgaga tctagggtat tccggtattc cttctcgaaa gttcttatcc ccctttcaca
      181 aagaattatc ttttcgtttc cactcgaaaa gatatactcg gcagatgata ggaactcggg
      241 taaagtagcc gacatacccc gtttcagcaa aacaggcttg tcaattttcc caagcgcatc
      301 cagcaagtta aaattttgca tgttgcgcgt ccccacctga aaaatatcta cgtacgggta
      361 catctgctca agctgcgaaa cctgcataac ctctgttact atttttattc cattttgatg
      421 gcagtgcttt gaaaaaaact ttagggcatc aatccccagg ccgcgaaagg cgtatggaga
      481 actccttggt ttgaatactc cgccccgcat aagcctgatg ttatgctgct tgaggaattc
      541 tactgttttt acaacctgat tctcatcctc tatggcgcaa ggcccagctg ctatgtagaa
      601 gttgtttctt gaaattacaa ccccatcgtc taggtcaatc tctgtgttag ctactctcca
      661 ctgccttgaa accaaaggat tcggatcggt tatgatatga acatcactca ccccagaaag
      721 acggccaatt attctgatgt caacctcggc attgcccata cacaccaggt agtaccggtt
      781 gaatgtttta actggagtag gatggtagcc cattttttta actgcttcta gaatgaaatt
      841 taactcggag gtgctgcagt ttttattaag ctgaattagc atggcaataa atttagcttg
      901 atactctaac cttacgaata aattgcgatg ttacgtcttc gaaatcaaca catgaagcaa
      961 tgctttttaa aaaagcactt ccaataattg ctccatcgca aaaacttgtg gcaaattcaa
     1021 atgtttcggc attgtgtatt ccaaagccaa ccagtatggg attgctgaga tttaagtttt
     1081 tcaaatgctt cagatattca gcttgaggac tgccaaatac agcttctttt ccggttgttg
     1141 acgatgtgct aaccgcatag atgaagccat cggagagctt gtctatcagc ctcattcgtt
     1201 catctgaagt ggatggacaa acaagggaaa tgaacggcag cgaatagcgc tgaaaagttc
     1261 tctggtagct ggcttgatat tcctccaggg gcagatcggg aattataagt ccgctaatac
     1321 caaccctgct ggcgtgtagg cagaattttt ccactccgta ctgcaggatg gggttaaggt
     1381 atcccataag cactaatggg attctcacct gcctgccaat tctttgaagc tgctcaaaaa
     1441 gtatggcaat actcatgccg ttggcaagcg ctacgctgct ggtgttctga attacgggcc
     1501 catcggccaa cggatcggaa tagggtattc caacctcaac caaatcggca ccagcatcct
     1561 gcagggccaa aataagcttg ttggttgaat cgagctgggg gtaaccagcc gtaatgaaaa
     1621 tggatagaat tttttttctt cctggctgca acaattccct taagctactc ataggttaac
     1681 gaatttggag tatgttttct ataattctca ctgtaggttt ccatgtcttt atcgccgcga
     1741 cccgaaaggt taaccacaac gatatcgttt ggcttaaaac ttaacatcga caaggcagca
     1801 agggcatgtg ccgattccag agcaggtata atgccttcag cttgcgccaa ctcgaaagag
     1861 gcgtttaagg cctgttcgtt tgttactgca aggtatctgg cacgtttggt tttattaagg
     1921 taggcatgaa gaggcccaac ccccgggtaa tcaagtccgg ccgataagga gtgagcctcc
     1981 aatatttggc catcatcatt ttgaaggagt agggttttac agccgtgaat tataccctca
     2041 gaacctttcg aaatggttgc ggcggttttg aacgtattaa cgccctcgcc tgctgcctca
     2101 actccaaaca gctctacttc cttgtcctga ataaaatggt agaatgcgcc ggcggcattg
     2161 ctacccccgc caacgcaggc aataacacaa cttggttttt caacaccctc ctgttcaagc
     2221 agctgggttc taatctcact acttataacc gattgaaatc ttgccaccat atcgggatat
     2281 ggatggggtc caatggttga acctataagg taatgggtgt tgtgagggtt gtcaatccaa
     2341 aaccgaattg cctcatttgt agcatcctta agggtttgac ttccagttgt ggcaggaata
     2401 acagtggcac caagcatttt catcctcaaa acatttggtt gttgacgctc aatatccttt
     2461 acccccataa agacagtgca tttcagcttc atcaaagcgc acatggtggc agctgcaact
     2521 ccatgctgac cggctcctgt ttcggcaact atgtggtttt tgccaaggcg ttttgcaatc
     2581 agcatttgcc caaccacatt attcagcttg tgagcaccgg tatggcataa atcctcacgc
     2641 ttaagataaa ttcttgctcc tttcactagc gaaagattcc gggcaaggta aagtggtgtt
     2701 ggtctcccga catagctggt aagcaactcg ttgaactcct tcctaaaatc ttcggagccg
     2761 ataatgttaa ggtagttttg ctgcagctca tggatgttgg catacagcat ttcggggaca
     2821 tatgccccgc cgtagctacc gtaataacct ctggaatctg ggtggtttgt cattttctac
     2881 tcaatttatt aacaaatgtg ttcagtagct ttatattttt tgaaccggtt tcatcttcaa
     2941 atctgctgtt gatgtcaact gcatgtagct gcgggtagct aagcctattc aggtagctgg
     3001 catctccggg tgcaatgccc ccgctaagga aaaatggtgt atgcaaatag tagcttgaaa
     3061 gcaaactatg gttaaactgc cgaccgttac ccccagcatg ccttcccctt gtatcgaata
     3121 aaaaaaacgc acagcaatcc tggtactgct caagatttaa gggcaatgca tcgttaatca
     3181 taaatgcttt tattactgga acatatcttt gaatttccct acactgctgt ggattttcgc
     3241 ttccgtggag ctgagcatgg gtaaagccgt acttctcaac caggcttatc actgtccggg
     3301 tgggctcatc aacaaaaacg gcaaccctac ctatatgctt gggaataagg ttaaaggaaa
     3361 tttgagcaac ccgcttggaa atgtctcgtg gagaggctgg atgaaatata aaccccaggt
     3421 agtccgggtt tagctgtacg attgcaggaa tgtcgcactt ttcagtaatg ccacaaatct
     3481 tcagcttcat gccattgaat ctttaacatt atgccacaat tccgtttaaa gtcaaagtat
     3541 ttaacctgtt gataaatgcc tggcaagcct taccggggtt agcttccctc ataaattttt
     3601 cgccaatcaa aaagccgtta aaacccacac ccttcattga aagcagcatt tcgggcgagg
     3661 taatcccgct ctctaccact ttagctacat caccgggaag gtctgaaata agagaagctg
     3721 catggttaat gctcacatga aaggtgttaa ggttccggtt gttgacgcca acaattgatg
     3781 cctgcggggg aagcttatca atacttttgc gggaatgaac ttcatggaga acttcgagtc
     3841 caagcgatga ggctatggcc gaaagtgcaa ccagctcttt ctttgtcaaa atttcagcaa
     3901 ttagcaggat ggcatcggca ccgatacttt ttgattcgac tacctggtac tcgtcaataa
     3961 caaaatcctt acgaagaatt gggcagtagt taaattttcg agcggcaata aggtcgtcgg
     4021 aagtgccccc aaagaactcc ctatcggtca ggaccgaaag ggctgatgcc ccggcctgca
     4081 tgtagtgcag gcaaacctgt tcgggtttgg caaactcgtt gatggttcct tttgatggcg
     4141 attttcgctt aaactcggct attatcccgt tcaagtcagg gcgcatgatg taggttttta
     4201 gcgaaagggg tggagtgttg aagtaaatgc ttctttcgag caacttcatt ggatatagct
     4261 cctttttttc ggctacctca cgttttttac atgctacaat ttcgtctagt ctgttcatgg
     4321 ttcactgcat tgccagtaat cgttttaact gctccattgc tctacccgac tctattgatt
     4381 cctgtgcctg atgggctgcc tcggtaattg aaatcccctt aaaacagcta atggctagag
     4441 ccgagttggc aatgattatg ctaattttac ccttatcggc ttggtttccc agcaccgaaa
     4501 ggaatatgcc ggccgattcc tccacagtgg ctcctccagc agcacacgaa gggcttagcc
     4561 tgctaaaccc aatactttcg ggcgaaaaac actcatcgcc catcttagaa aacactttca
     4621 catcgctggt gagggatatt tcgtcgtacc cgtcgagact atacaccacc ctgtagtcgg
     4681 tcaggcatcg gttgaaaagg tagctgaaca tcctgccgac ctcggcatta aacactccgc
     4741 taagctggta tttaggacga gcggggttta ttaatggccc aagcatgtta aaaaaggttt
     4801 tcacgccaag ctgccgcctg acagggccaa tagccttaag cgcaggatga aacagggggg
     4861 catgcaggaa gcaaacattg gtttgctcaa tttcgcgctt cagcttatca gggttgctgc
     4921 taaaactata gccgaggtat tccattatat tggacgatcc ggttattgac gaagcgctat
     4981 agttgccatg cttggcaaca ggaacgttgg ctccggctac aacaaaagtt gatgcagtgg
     5041 aaatgttgaa agtgtttttc ccatcaccac cagtgccgca aaggtcaatt acctccccgt
     5101 taagtagctt tacctcctgc gaaagctcaa gcagcgcctc ccggaagcct ataagttcat
     5161 ccaggctaat gctccgcata acatacgccg taagcacagc tgacatttgc gcctcattca
     5221 ccgcgccacc ggcaatgcca tgcataaggt ctcttgcctc taaatggctc aatcgttgcg
     5281 actccagcaa cttgttaaga atacttttca actccataaa tctgatactt ttctgtttag
     5341 ctgcgcttta aaaagctgaa acaataattt tcagagcacc cttggctgcc tattttaaaa
     5401 ctgacctagc cagttgaaca ctacctgctt acccatgggg gtaagaatgg actcggggtg
     5461 aa
//
LOCUS       DAOSHM010000107         5417 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_13366_length_5417_cov_21.617120, whole genome shotgun
            sequence.
ACCESSION   DAOSHM010000107 DAOSHM010000000
VERSION     DAOSHM010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 5417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5417
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13366_length_5417_cov_21.617120"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(178..1707)
                     /gene="gltX"
                     /locus_tag="PK839_10235"
     CDS             complement(178..1707)
                     /gene="gltX"
                     /locus_tag="PK839_10235"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HOG73153.1"
                     /translation="MENRRVRVRFAPSPTGPLHIGGVRTALFNYFFARQHNGDFILRI
                     EDTDSQRFVPGAEEYINEALGWCGIKIDEGVKEGGPHAPYRQSERRQIYRQFADRLVA
                     SGNAYYAFDKPETLQKLRNEAENRGEAFTYNYANRNSMENSHTLGEDEVRNRIEGGDQ
                     WVIRYKMPADEEVVMHDIIRGEIRVNTSTLDDKVLFKSSDMLPTYHLANVTDDYLMEI
                     SHVIRGEEWLPSLPLHVLLYRALGWEDKMPQFAHLPLLLKPTGNGKLSKRDGDKMGFP
                     VFPLLWKAENGEVYRGYREDGYFPEAFVNLLALLGWNPGTERELFNMEELIQIFSLER
                     VNKSGARFDPEKAKWFNHQYLIRKGNDEVANLFMPILEQYGVQVQMDKLVRIVGLVKE
                     RVNFIHELWGQTSFFFERPLNYNSQTVAKFWNNDSSVMMNILVEKLSGISTWTALNIE
                     QSVKELIGEKGYGMGKVMNTIRLALVGESKGPGVADICEILGKEETLQRIRQAVLILG
                     K"
     gene            complement(1803..3158)
                     /locus_tag="PK839_10240"
     CDS             complement(1803..3158)
                     /locus_tag="PK839_10240"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOG73154.1"
                     /translation="MFSCISQDSVTRNQGHEVRDLADVLAEGKLVAVTDYNSASYYIY
                     KGEPMGYQFDMLRDFASFLGVKLELIAENDIQSSIEMLNSREVDLIANNLTITPERME
                     ELSFTLPHGETRQVIVQRKQPKPGEKGQTYIRSSQELAGKIVYVQRGSVSAKILKNIT
                     EKNGRSTVLVELPGYDAFQLIDMVAQGKIDYVVCDEVEAKVKVAVYSNLSCEIAVSNL
                     QPTAWAVRQSSPELLKRINLWLENYLSTSKYARIRSKYYENPLQARRMSNDYFYVYQG
                     KVSQWDEYFKHNSEKIGWDWRLLASMVYQESHFDHNAVSPRGAVGLMQFMPTTADYFG
                     IALNEMPIRQIEAGVGYLKWIEDRLSSFNIPKKEEVKFVLAAYNAGIGHVIDARNLAR
                     KYGRNPNVWDDNVEYFISNKAQFSGDPVVKYGSLKGKETTRYVEEILERYQHYQNIVE
                     K"
     gene            complement(3297..>5417)
                     /locus_tag="PK839_10245"
     CDS             complement(3297..>5417)
                     /locus_tag="PK839_10245"
                     /inference="COORDINATES: protein motif:HMM:NF025486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG73155.1"
                     /translation="FFYYFFRIFAPCMLNATFKYSTISTCFLLVNFCSLKAQIVDSTS
                     HNIDSNGIVIDSTANTDTSRLTKSNFSKFNFESVPTSKIDSTVVYPNIYDVLGVGGSK
                     YTALAWKIDKNTFDLSICPAVDTSLFLNHLILPGQSNLFAQTYLGNMGSPIQSDNFFD
                     RPIDKPFLFSKNYQLYQHDILSHKQFNVRSPHTRLFYSTGGKRREAEQILRVLHTQNV
                     NRYLNFGVEYDYFSTKGIYENQLTRNNMVSVFASYYKKRISAQATFGYTYIRNQENGG
                     LVDDKFIVDTVLEPSLVPFRLKNASSEVRQRSFAAVVGYDILTIKKKIKDEDGNDTLV
                     DRPLLTSKLVFDANRHTRVYSDSEGDSTYYSNFFISTAGTHDSVYMVTYTTTLLAELS
                     QLARFPGVPGLRFWVSNTTGSYYYYTPQDFIYNRSNDKLTTNHLGVGVFSKSPYLSYS
                     GALRFYVNGYRSNEKLLFGGITISPWKSTEMPFIKGSITFQDRQPDLFVRNYFSNHYA
                     WSNNFEREKWLTVSGVLGADRYKFEIGYNVANVVNYIYFDTTGRPAQASNVAITSAYV
                     QKVFKVRGLYMVGKVLWQANTNSEVLSLPTLSGFGSIYYEYPLVKNVLTGQLGISVFY
                     RTKFYADSYNPSTGQFINQREKKIGNYPFADVFANFKWKRANIFLKFEHVNQGLFSNE
                     YFTALHYPANRRIFKFGVSWMFYD"
BASE COUNT         1542 a         1236 c         1096 g         1543 t
ORIGIN      
        1 gggaggctgc tggcttaagc accttcgaca aagcggccaa acggcttgag agctcaacag
       61 tgccggaaat tattcaggta gttgttttgg gcaagccatc ctactagttg gcgcaacgtt
      121 taccggagta aacacagtaa tgagagaatt taccctttgg cttaaccccc taaagctcta
      181 cttacccaga attaaaacgg cctgcctaat tcgctgtagg gtttcctctt tgccaagaat
      241 ctcgcaaata tcggcaacgc cagggccctt tgattcgcca acaagcgcaa gccgaatggt
      301 attcataact ttacccatac catagccctt ttctccaatc agctccttta ccgattgctc
      361 aatgttgagc gctgtccatg ttgaaattcc ggatagcttt tctaccagga tattcatcat
      421 cacggaagaa tcattgttcc aaaacttggc aaccgtttgg ctattgtagt tcagtggcct
      481 ttcaaaaaag aagctggttt ggccccaaag ctcatgaata aaattgactc gctccttgac
      541 cagcccaaca attcggacaa gtttgtccat ttgaacctga acgccatact gctccaaaat
      601 aggcataaaa aggttggcaa cctcatcgtt gcccttgcgg attaagtact ggtggttgaa
      661 ccactttgcc ttctcgggat cgaatcgggc accggactta tttacccgct cgaggctgaa
      721 aatttgaatt agctcctcca tattgaagag ctccctttct gttcccggat tccatccaag
      781 cagggcaagc aggttgacaa atgcttcagg aaaatagcca tcctcacggt agcctctata
      841 cacctcacca ttttcggcct tccaaagtag aggaaacaca ggaaaaccca ttttgtctcc
      901 atcacgcttg cttagctttc catttccggt aggtttaagc agtaggggca aatgggcaaa
      961 ctgtggcatt ttgtcctccc atccaagggc gcggtataac aaaacgtgca ggggtagcga
     1021 tggaagccat tcctctcctc tgataacatg cgaaatttcc atcaggtagt catcggttac
     1081 attggccaga tggtaggttg gaagcatgtc gctactctta aacagcacct tatcgtcaag
     1141 cgtggaggtg ttaaccctaa tctcgcctcg aatgatgtcg tgcatgacaa cctcctcgtc
     1201 agcaggcatt ttatacctaa taacccactg gtcgccgcct tcaattcgat tgcgaacctc
     1261 atcctcgcct aaagtatgcg agttttccat ggagttgcgg ttggcataat tataggtaaa
     1321 agcttctccc ctgttttctg cctcattgcg gagtttctga agtgtttcgg gtttatcaaa
     1381 cgcatagtaa gcatttccac tagccaccag cctgtcggca aattgtctgt aaatttgcct
     1441 acgttcgctt tggcggtagg gagcatgagg cccaccctct ttaactcctt cgtcaatttt
     1501 aattccgcac cagccaagcg cctcgttgat atactcctcg gcacctggta caaaccgctg
     1561 agaatcagta tcctcaattc taaggataaa atcgccgtta tgctggcggg caaaaaagta
     1621 gttgaacagg gctgttctaa cccctccaat gtgcaacggt ccggttggac taggggcaaa
     1681 acgaacccgt actcttcggt tctccatttc aaattttttg tgaggtcaaa attatgaata
     1741 attagtgggc tataaacgtc aacccgaaaa acaagtacgg ctggctacaa aaaatctacc
     1801 atttactttt ccacaatgtt ttggtagtgc tggtaccttt cgaggatttc ttcaacatac
     1861 cgtgtagttt ccttaccctt aagacttcca tatttgacta cgggatcgcc agaaaattgg
     1921 gccttattgg atatgaagta ctcaacatta tcgtcccaaa cattagggtt acggccatac
     1981 tttctggcca agttacgggc atctataaca tgacctattc cggcgttata agcagccagc
     2041 acaaatttaa cctcttcttt tttagggata ttgaagctgc tcagcctgtc ctctatccat
     2101 ttaaggtacc caacaccagc ctctatctgc cttataggca tttcattaag ggctataccg
     2161 aaataatcgg cagttgtggg catgaattgc ataagaccta ctgcgcctct gggtgatacg
     2221 gcattatggt caaagtgcga ttcctgataa accatggagg ctagtaaccg ccaatcccaa
     2281 cctatctttt cactattatg tttaaagtat tcatcccatt gcgaaacctt cccctggtaa
     2341 acataaaaat agtcgttgct catgcgccgt gcctgaaggg gattttcata atattttgaa
     2401 cgtatccggg catacttcga ggttgacagg tagttctcca gccacaaatt aatccgcttg
     2461 aggagctcgg gtgatgattg acgaactgcc cacgctgtcg gttgaagatt actaactgca
     2521 atttcgcagc taaggttact gtaaacggca accttgactt tggcttcgac ttcgtcgcag
     2581 accacatagt caatcttacc ctgcgcaacc atatctatta gctgaaaagc atcatagcca
     2641 ggaagctcaa ccaggacagt actcctccca tttttttcgg taatattctt taatatttta
     2701 gctgataccg atccccgttg aacgtaaaca atttttcctg ccaactcttg agagctgcga
     2761 atataggttt gtcctttttc tccgggtttt ggctgcttcc tttgaactat aacctgacgg
     2821 gtttcgccat ggggtaaggt aaagcttaac tcctccatgc gttcgggggt tatggtaagg
     2881 ttgttggcaa ttaggtcaac ttcccggcta tttagcattt caatagagga ttgaatatcg
     2941 ttctcggcaa tgagctcgag ctttactccc aaaaatgagg caaaatcgcg tagcatgtcg
     3001 aactggtagc ccatgggctc tcccttgtaa atataataac ttgctgagtt ataatctgtt
     3061 actgccacca acttaccttc agccagcaca tcggccaaat ctctaacctc atggccttgg
     3121 ttcctggtaa cactatcctg gcttatgcag gaaaacatta ttagggaaaa caaaaataaa
     3181 ctccacttta tgcacttgta aacaaaagat gaccgcatag ctcgtccttt agggttaaac
     3241 ctgtaaaaaa gcaaaattag gttaatcaac tgataatcag agatggattt tgataactag
     3301 tcgtaaaaca tccacgaaac tccaaacttg aaaattcgac ggttggcggg atagtgaagc
     3361 gctgtaaagt attcgttact aaacaggccc tggttaacat gttcgaactt cagaaagatg
     3421 ttggctcgtt tccatttaaa atttgcaaac acatcggcaa aggggtagtt gccaattttc
     3481 ttctcccttt ggttgatgaa ctggccggtc gatgggttgt agctatcggc ataaaatttg
     3541 gttctataaa atacactaat accaagctgg ccagtcaaaa catttttcac cagcgggtac
     3601 tcgtagtaaa tcgatccaaa acctgaaaga gttggtaggc taagcacttc tgaattggta
     3661 ttcgcctgcc aaagcacctt cccaaccatg tacaaccctc ttactttgaa taccttttgc
     3721 acataagcag atgtaatggc aacattggaa gcctgagcag gtctgccagt agtgtcaaag
     3781 tagatatagt taacaacatt cgcaacatta tatccaattt caaacttgta ccggtcagca
     3841 ccgagcactc ctgaaactgt caaccacttc tccctttcaa aattattgct ccaggcataa
     3901 tgatttgaaa agtaattcct aacaaacaaa tcgggttgcc tgtcctgaaa agtaatactt
     3961 cccttgataa aaggcatttc ggttgatttc cacggtgaaa tagtgatacc cccgaatagt
     4021 aacttttcgt ttgaacgata accattaacg tagaacctta acgcacctga atacgacaag
     4081 tagggagatt tactaaacac tccaacgccc aaatggttgg tagtaagctt atcattactc
     4141 ctgttataaa taaaatcctg aggtgtatag taatagtagc tgccagttgt gtttgatacc
     4201 caaaatctta aaccaggaac tccaggaaaa cgggcaagtt ggctaagttc agccaataat
     4261 gttgtagtgt aggttaccat atacaccgag tcgtgtgttc ctgctgtact aataaagaaa
     4321 tttgagtaat aagtagagtc tccttcacta tcggaatata ccctggtgtg ccggttagca
     4381 tcaaacacca atttagacgt caacaatggc ctatcaacca gcgtgtcgtt cccatcttca
     4441 tctttaatct tcttcttgat agtcaggata tcataaccaa ctacagcagc aaaacttcgt
     4501 tgccttacct cgctggaagc atttttcaac ctgaaaggta cgagtgaggg ctctaaaaca
     4561 gtatctacaa taaatttatc atcaaccagc cctccgtttt cttggtttcg tatgtaggta
     4621 tatccaaagg ttgcctgagc tgaaattctt tttttgtaat agctggcaaa tacggaaacc
     4681 atattatttc gcgttagctg gttttcatat attcccttgg tagaaaagta gtcgtattcc
     4741 actccaaaat ttaaatacct gttgacattt tgggtatgaa gcactcttaa aatctgttcc
     4801 gcctcccgtc ttttaccgcc agtagaatag aaaaggcggg tatgagggct tcgaacgtta
     4861 aactgcttgt gcgatagaat gtcgtgctgg taaagctgat aattttttga aaatagaaag
     4921 ggtttgtcaa taggtctatc aaaaaaatta tcgctttgta taggcgagcc catatttccc
     4981 aggtaggttt gagcgaacaa gttgctttga ccaggcagaa tcaggtggtt taaaaaaagg
     5041 gaggtatcca cagccgggca tatcgacaag tcaaacgtgt ttttatcaat cttccatgcc
     5101 aatgcggtat atttactgcc gcctactccc agcacatcat aaatattagg gtaaacgact
     5161 gtcgaatcaa ttttagaagt tggtacactt tcaaagttaa atttactaaa gttactcttc
     5221 gtcaacctgg aagtatcggt attagcggta gaatcaatta caataccatt actatcgata
     5281 ttatgagaag tagagtcaac aatttgagcc tttaatgaac aaaagttgac taaaagaaag
     5341 caagttgaaa tggtggagta tttaaaggtg gcattaagca tacagggtgc aaatatacga
     5401 aaaaagtaat aaaaaaa
//
LOCUS       DAOSHM010000108         5374 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_13523_length_5374_cov_7.204926, whole genome shotgun sequence.
ACCESSION   DAOSHM010000108 DAOSHM010000000
VERSION     DAOSHM010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 5374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5374
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13523_length_5374_cov_7.204926"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="PK839_10250"
     CDS             complement(<1..203)
                     /locus_tag="PK839_10250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG73156.1"
                     /translation="MMKRVLFIEPIVAHYRRDTYRLIFSDSRFEFALLAGDSYLDIKP
                     EKVEKTIISTYRKFTLLGEEFYYL"
     gene            complement(209..>1468)
                     /locus_tag="PK839_10255"
     CDS             complement(209..>1468)
                     /locus_tag="PK839_10255"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HOG73157.1"
                     /translation="LAIFRKSEILQNASILVSGTIMAQAIPILLQPFFRRYFSPHDFG
                     VYSVYSSLIGILLVISSFRYEQAIVLPKRNRDASVLVTSSILFSFVFSFLLFIAIILF
                     RKGIGEALNLDDSDGFILYLIPIGVFLLSVFQVFNFWLIRQKGFMKISVNKLVRRIVE
                     GGSQSAAALTHYPMGLVLGDVAGQVGNAVTSAYQSVRCGYSPRHFSIVRSKYLLDSYS
                     DFPRYSLIPALCSTIGFLLPVVLINRYYLPEQAGFFDLAKLILSVPMALVATSISSVV
                     LQKVSESYRLGKSFVGELKPLIAFVFIASVVEVLLIVFAGKGIFRVFFGKVWETSGII
                     SKWLVWPFALNFITSSFTSIFVALNRIRWQSLWQVAYFLLIISLPLFRNFTFYGFIRV
                     YASLEILANLAMVTLLTIVVIKYEKKF"
     assembly_gap    1469..1568
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1569..2344)
                     /locus_tag="PK839_10260"
     CDS             complement(<1569..2344)
                     /locus_tag="PK839_10260"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG73158.1"
                     /translation="MTIVIVILLLACIVSFTLAAFIRRMAIRLRLMNESNARSLHSGS
                     IPIGGGLAIIAVWYIGTAVFFWLGLMDKSLFYALLCGLVLAGVSLLDDIIQVKPIIRL
                     IVHFGVATASLYLLGGLRKPVTPGIDILSIPAVINPLAVVGMVWFINLYNFMDGADGF
                     ASVEAISIALVLFAFTGSMELLLLVSAVLGFLYWNWPKAKIFMGDVGSTQLGFILVVL
                     GINYHNTLDFSIFNWLMISSPFWFDATLTLFRRWRNKEKL"
     gene            complement(2347..3225)
                     /locus_tag="PK839_10265"
     CDS             complement(2347..3225)
                     /locus_tag="PK839_10265"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOG73159.1"
                     /translation="MPRILIIGKNSFIGKNYREYSKNSSIDEVDAKQYKPEELNFEGY
                     DVVLHLAAIVHQSGNIGLEEYTRVNTHFPVALAEQAKMAGVGQFIFMSTSKVYGKYNP
                     AMGSWTEDSPCSPANYYGISKLRAELELLKLNDISFQVSIIRTPMVYGRGVKANMLSL
                     IKLVDGLPLLPFKRVNNRRSLTFAGNLAAYIDRIIELKTQGVFIAMDAYSPSTEELIV
                     SIAAALGKNTRLFSPGRLALNLGKHFFRGYYERLYLDEVLDNTKTLELLSFTPPYSLE
                     EGMADTISSYLQLKEK"
     gene            complement(3274..>4146)
                     /locus_tag="PK839_10270"
     CDS             complement(3274..>4146)
                     /locus_tag="PK839_10270"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HOG73160.1"
                     /translation="RVGGIMANNTYRAQFIYENIQIQNNVIHAAYLNGVKKLLFLGSS
                     CIYPKDSPQPIKEEYLLTSPLEYTNEPYAIAKIAGIKMCESYNLQYGTNFISAMPTNL
                     YGPGDSYDLAKSHVLPALIRKMYLGKCLSENRWEDLRHDLNKWPVDGVDGESSKNEIF
                     AVLGKYGISETSITFWGSGKPMREFLHSDDMADASVFLMENVDFDALKGHGEIRNTHI
                     NIGTGKDISITNLAGMVKEIAGYHGSIRWNTSMPDGTYRKMLDVGKLHSLGWHHSIEL
                     EKGIGQAYTDYANS"
     assembly_gap    4147..4246
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4247..5118)
                     /gene="gmd"
                     /locus_tag="PK839_10275"
     CDS             complement(<4247..5118)
                     /gene="gmd"
                     /locus_tag="PK839_10275"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005469633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOG73161.1"
                     /translation="MADKLKKALITGITGQDGSYLAELLLGKGYEVHGVIRRASTFNT
                     QRIDHLYSDRHNKDVSMFLHYGDMTDTSSLNRLLEKVAPDEIYNLAAQSHVKVSFEVP
                     EYTAEVDGIGTLRFLDAIKDVGLRTRFYQASTSELYGLVREVPQNETTPFYPRSPYGA
                     AKLYAYWITVNYREAYGIFASNGILFNHESPRRGETFVTRKITLAATRIKAGLQDKLY
                     LGNLDARRDWGYAPEFVEGMWRILQHDTPGDFVLATGETHSVREFVELAFKLIGMDVE
                     WKGVGINETGVDKNT"
     gene            complement(5111..>5374)
                     /locus_tag="PK839_10280"
     CDS             complement(5111..>5374)
                     /locus_tag="PK839_10280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG73162.1"
                     /translation="DYPHSPFLSVMLYSGIFGLIFYIWLLLGAVKYYWTYRRDYWPFG
                     LAFVVAFFFAFFSSNNPFEPAVLAVFTTIPYFAHYFYLVEKHG"
BASE COUNT         1575 a         1207 c         1047 g         1345 t
ORIGIN      
        1 aggtagtaga attcctcacc gagtagcgtg aacttcctat atgttgatat gatggttttt
       61 tcaacctttt caggtttaat atccaggtat gaatcacctg ccagcaatgc aaattcgaag
      121 cggctatcac taaagatcaa ccggtaggta tcccttcggt agtgggcaac aatcggctct
      181 atgaaaagca cacgcttcat catgtcattc aaaatttttt ctcgtattta ataacgacaa
      241 tagtcagcag cgtaaccatt gccagattgg caagtatttc caaactagca taaaccctaa
      301 taaagccgta aaacgtgaaa tttctgaata gcggaagtga aataatcagc aggaagtagg
      361 caacttgcca caagctttgc cacctgattc tgttaagcgc cacaaaaata gaggtaaatg
      421 atgaagtaat aaagttcagg gcaaaaggcc aaaccagcca tttcgaaata attcccgatg
      481 tttcccacac tttcccaaaa aacaccctaa aaatgccctt acccgcaaat acaatcagca
      541 gtacctcaac tactgaagct ataaaaacaa aagctatcag tggtttaagt tctcccacaa
      601 aggatttccc cagacgatag ctttccgaaa ctttttgtag cactacgctc gaaattgagg
      661 tggcaactag tgccattgga accgatagga taagtttagc cagatcaaaa aaacctgcct
      721 gttcgggcaa gtagtacctg ttaatcagaa ctaccggcaa taggaatcca atagtgctgc
      781 atagggcagg aataaggctg taacggggaa aatcggaata gctatcgagt aaatatttag
      841 atcgaactat gctgaaatgc cttggagaat acccacacct taccgattgg taggctgaag
      901 taactgcatt tccaacttga ccggcaacgt ctcctaaaac aagacccatg gggtaatgag
      961 taagggctgc tgctgactgt gagccaccct ccacaattct acgaaccagc ttattaaccg
     1021 aaatcttcat aaatcctttc tggcgaataa gccagaagtt aaacacctga aaaacactca
     1081 aaaggaatac cccaatagga attaggtaaa gaataaatcc atcagaatcg tctaggttaa
     1141 gcgcctctcc tatccccttt cgaaatagga taatagcaat gaacagcaga aagctaaaca
     1201 caaaactaaa aagaattgat gatgttacga gcactgaggc gtccctattt cttttaggta
     1261 gcacaatagc ttgttcgtaa cggaagctgg aaattacaag caaaattcca attaaactgc
     1321 tgtaaaccga gtaaacccca aaatcgtgag gtgagaagta gcgacgaaaa aatggttgca
     1381 gcagtatggg tatggcctga gccataatgg taccgcttac cagtattgaa gcattctgaa
     1441 ggatttcaga ttttctaaaa atagctaann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnct caacttttcc ttatttcgcc aacgtcgaaa aagtgtaagg gttgcatcaa
     1621 accagaatgg tgacgaaatc atcaaccagt taaaaattga gaaatcaagc gtgttgtgat
     1681 agtttatccc cagcacaacc agaataaaac ccagctgggt acttcccaca tcgcccataa
     1741 aaattttggc ttttggccaa ttccaataca aaaatcccag cacagccgaa actagaagta
     1801 aaagttccat gcttccagtg aaagcgaaaa gaactagggc gatagaaatt gcctcaaccg
     1861 aggcaaaccc gtcagcgcca tccatgaagt tgtacaggtt gataaaccag accattccca
     1921 caacggccag cggattgatt actgcgggaa tgctaagtat atcaataccg ggggttaccg
     1981 gtttgcgtaa acctcccagc aggtataaag aggcagtagc cacaccaaaa tgaactatca
     2041 gtctaattat gggctttacc tgaattatat catcaagcag gctcacgccg gcaagtacaa
     2101 gcccacaaag caaggcataa aaaaggcttt tatccataag gccaagccag aaaaaaactg
     2161 ccgttccaat ataccaaaca gcaataattg caagcccccc accaatagga atactgcctg
     2221 agtgtaagct tcgagcattc gactcgttca taagtcttaa ccgaatggcc atacgcctga
     2281 taaaagcagc cagtgtaaaa gaaacaatgc aggccagcaa taaaataaca attactatgg
     2341 tcataactat ttttctttaa gttgcagata agaacttatt gtatctgcca taccctcttc
     2401 aagggaataa gggggagtaa aggaaagcaa ttcgagtgtt ttggtgttgt ctagcacttc
     2461 atctaaatat aatcgttcgt aatatcccct aaaaaaatgt ttaccgaggt ttagtgctaa
     2521 tctccccgga gaaaaaagcc tggtattttt cccaagtgcg gcagcaattg atactatcaa
     2581 ctcctcagtg ctgggggaat aggcatccat tgcaatgaac acaccctgag ttttaagttc
     2641 tataatccga tcaatgtagg ccgccaaatt accggcaaag gtcaggcttc gccggttatt
     2701 aaccctttta aagggtaaaa gcggaagccc atccaccagc ttaatcaggc taagcatgtt
     2761 ggcctttaca cccctgccat aaaccatggg tgttcgaatt attgaaactt ggaaacttat
     2821 atcattcagc ttcaataatt ccagttcagc ccgaagtttg ctgatcccgt agtaattagc
     2881 tggactacag ggtgaatcct ctgtccaact tcccatagct gggttgtact tgccgtaaac
     2941 cttagaagta ctcataaaaa taaactgccc aacaccagcc attttggctt gctcagccag
     3001 ggcaactgga aagtgtgtat tcacccttgt gtactcctcc aacccaatgt ttcctgactg
     3061 gtgcactatt gctgctaggt ggagaaccac atcataccct tcgaaattca actcttcggg
     3121 tttgtactgt ttagcatcaa cctcatctat tgaggagttc ttgctgtact ccctataatt
     3181 ttttccaata aagctgttct taccaataat cagaattcgt ggcatagtgg gtaatgatta
     3241 atatatcctg agtaattggt tgcccattgt cgtttagcta ttggcataat ccgtataggc
     3301 ctgtccgatt cccttttcca gttcaataga atggtgccac cccagtgaat gcagcttacc
     3361 tacatccagc atcttacggt atgttccatc aggcatagag gtattccacc taatgcttcc
     3421 gtgataacca gctatctcct taaccatccc tgccaggttg gttatagata tatccttacc
     3481 cgttccaatg ttaatatggg tattccgtat ttcaccgtgg cctttcaggg catcaaaatc
     3541 aacgttttcc attaaaaaca ccgaggcatc ggccatatcg tcagagtgaa ggaattcgcg
     3601 catgggctta ccgctgcccc aaaaggtaat agaggtttcg gaaataccgt atttgcctag
     3661 cacagcaaat atctcatttt ttgacgattc tccatcaacc ccgtcaaccg gccacttgtt
     3721 aagatcgtgc ctcaggtctt cccagcggtt ctcggacaaa catttgccaa gatacatctt
     3781 gcgaataagc gccggaagca catggctttt ggccaaatca tagctatcgc cgggaccata
     3841 caggttggtt ggcatggctg atatgaaatt agttccgtat tgcaggttgt acgactcaca
     3901 catcttaatt ccggcaattt ttgcaatggc atagggctca tttgtatact ccaacggcga
     3961 ggtaagaaga tactcctcct ttatgggctg gggggaatcc ttaggataaa tgcacgagct
     4021 gccaagaaac agcaattttt taacgccgtt aagataggct gcgtgtatta cattattttg
     4081 aatctggata ttctcataaa tgaactgcgc tctataggtg ttattggcca ttatacctcc
     4141 aaccctnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngtat ttttatccac
     4261 gcctgtctca tttattccaa cccccttcca ttcaacgtcc attcctatca atttgaatgc
     4321 tagttcaaca aactccctaa ccgagtgggt ttcgccggtt gccagaacaa aatcaccggg
     4381 cgtatcgtgc tgcaggatgc gccacattcc ctccacaaat tctggtgcgt atccccaatc
     4441 gcggcgggca tcgaggttcc ctaggtatag tttatcctgt aagcctgcct ttatacgagt
     4501 agcggccaag gttattttcc gggtaacaaa agtttccccc ctacggggcg actcgtggtt
     4561 gaacagtata ccattgcttg caaaaatccc gtaggcctcc ctgtagttta ccgtaatcca
     4621 gtaagcataa agtttagcag caccataggg gctacgggga taaaacggag tagtctcatt
     4681 ctgaggtacc tccctcacca aaccgtatag ttcgctggtg gaagcctggt aaaagcgcgt
     4741 tctgaggcca acatccttaa tcgcatctag aaaacgcaac gtgccaattc catccacctc
     4801 ggcggtatat tccggcacct caaacgaaac cttcacgtgg ctttgcgctg caagattgta
     4861 aatctcgtct ggagcaacct tttcgagcag cctattcagg ctgctggtgt ccgtcatatc
     4921 accgtagtgt aggaacatgc tgacatcctt gttatgcctg tcgctgtata gatggtcaat
     4981 tcgttgggtg ttaaaagttg aggcgcggcg tataacgcca tgaacctcat atcctttacc
     5041 taaaagcaac tcggccagat acgatccatc ttgtccggtt attccggtta tcaatgcttt
     5101 ttttaatttg tcagccatgt ttttcaacca agtaaaagta atgtgcaaag tagggaatag
     5161 ttgtaaatac agccaataca gcaggttcga atggattatt tgagctgaaa aatgcaaaaa
     5221 agaaggctac aacaaatgcc agtccaaacg gccaataatc cctgcggtaa gtccagtagt
     5281 atttaacagc accaagtaaa agccaaatat agaaaataag accgaaaatt cctgagtaga
     5341 gcattacaga aagaaagggg ctgtgtgggt aatc
//
LOCUS       DAOSHM010000109         5261 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_13946_length_5261_cov_5.001537, whole genome shotgun sequence.
ACCESSION   DAOSHM010000109 DAOSHM010000000
VERSION     DAOSHM010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 5261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5261
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13946_length_5261_cov_5.001537"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(117..1520)
                     /locus_tag="PK839_10285"
     CDS             complement(117..1520)
                     /locus_tag="PK839_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008779674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HOG73163.1"
                     /translation="MRTVPLMIKSVEMQADTFTPIGIYLRLRDIFPQCLLLECSDYST
                     RSEAFSYICLQPFAGIEVGKSRTYTYFINQQREEHTLKDAEIIAFVDSFFKSFTLEGS
                     NIHPETAGFYGFTAYDAVNFFDSVNINTLDDSIPLLRYDFYQVILAFNHFNNTLTLVE
                     YTAPGMNAIGDRVLSLLANRNAPVFPFVAAAEEESNMSDEQFMAMVEQGKLHCARGNV
                     FQIVLARQFSRGFSGDDFNVYRALRSINPSPYLFYLDYLNYRIFGSSPEAHLKVNSGI
                     ATINPIAGTAVRTGNADMDKALAANLLQNPKENSEHCMLVDLARNDLSRYAHNVTVEK
                     YREIYTYSHVVHMVSNVRGTLSTSYSPYALMASTFPAGTLSGAPKYKAIQLISEIEPG
                     PRGIFGGAVGFIGLDGNLNHAIIIRSFLSKMGVLKYQAGAGVVNGSTPEGELAEVNSK
                     VTALRMAVDKASVNFSN"
     gene            2201..3049
                     /locus_tag="PK839_10290"
     CDS             2201..3049
                     /locus_tag="PK839_10290"
                     /inference="COORDINATES: protein motif:HMM:NF012998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HOG73164.1"
                     /translation="MYQTVAIQGIKGSFHHQAARECFGLDCTVTECLTFTDLVETVLS
                     GVAGYGIMAVENSLVGAILPNYGLIRESSLTIIGEVYLRIEQNLLALPGQSVDEIKEV
                     YSHPMALQQCGVFLSMLKDVRVVASDDTALSAKQVAEKGQKGVAAIASMDAAREYGLE
                     VVQSGIESYKDNFTRFVALTQNPNVACSHMANKASIAFALPPKPGSLQKALQVFSRYG
                     MNLTMLHSLPQVGQCWEYFFHADLVFRHIDDLNLGLQSLNRCTTFTKVMGIYHSESLN
                     NNSTTT"
     gene            3061..3978
                     /locus_tag="PK839_10295"
     CDS             3061..3978
                     /locus_tag="PK839_10295"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HOG73165.1"
                     /translation="MDLQKKCLAIVGTGQIGCSIGMAVKKHVALVTGTDLNQKHLQEA
                     VQLGLVDVALTLREAIAYASIIVVATPVDVAIPLLAQILDLIDSNTLVVDVGSTKLQV
                     CNALRQHINRRNYVAAHPMAGSSVQGPKGALPKLFDGAKTFICEPQLSSDYALDTAKE
                     LFHCLNSRVEFIDPDEHDRLVGLVSHLPQALAYSLCNVVKLFDAEGFDWKGAAATAFD
                     STTRLAGSSSNVWLPIMNQNRENLLKYITATVGELHKLQQYLESNNIPALETYIDIAN
                     QVRNQLEESKKTPTSNSNGKRSTSTATAA"
     gene            3941..5059
                     /locus_tag="PK839_10300"
     CDS             3941..5059
                     /locus_tag="PK839_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-deoxy-7-phosphoheptulonate
                     synthase/chorismate mutase type II"
                     /protein_id="HOG73166.1"
                     /translation="MEKEALQQQPLPDSWTISTVKPLIIAGPCSAESPEQLRCVAQEL
                     KRNSSVSYMRAGVWKPRTDPNSFQGYGAEALEWLRSVKQEVGMRFATEVGCSQHVLEA
                     LKYGVDLVWIGARTVANPFAMQEIADALKGVDIPVMVKNPLNPDIELWDGAICRLLNA
                     GIRKVGAIHRGFSLIKKSVFRNPPLWEIPLQLKQRYPNIPIICDPSHITGSRNLVPLV
                     AQRAKDLNFDGFMVEVHPYPDIALSDPAQQLTPQQFDNLLEMLLNNNTPGCSNSLDML
                     DELRAEIDVMDDLLVWILSGRMELAGKIAGIKKMKNLQILQAERWNQIQERLLAKTSE
                     SGLRPQFISSLYHTIHKESLSIQSSLMKDKGKNINHSH"
BASE COUNT         1523 a         1169 c         1195 g         1374 t
ORIGIN      
        1 tcccgaattc ccggacatag tgaagcaggt tgtaggtaaa cgaatcgtag ttgtcgagta
       61 gtagaattag cgccatagtc aaaattgatt aaattacttt tgggttttca cgcattttaa
      121 tttgaaaaat ttactgaagc cttatcaaca gccattctga gggcagtaac ttttgagtta
      181 acctcggcca gctcgccttc gggggtggag ccattaacaa cacctgcccc ggcttgatac
      241 ttaagaactc ccatcttgct aagaaaagaa cgaatgatga ttgcatggtt gaggttgcca
      301 tctaatccga taaaccccac agcaccacca aatattccac gcgggccggg ttcaatctcg
      361 ctaattaact gaatagcctt gtacttgggt gctccactta gcgtccctgc aggaaaggtt
      421 gaagccatca aagcgtaagg actgtagctg gtcgacaatg ttcccctgac atttgaaacc
      481 atatgaacca catgagagta ggtatatatt tccctgtact tttcaacagt aacgttgtgc
      541 gcatacctgc tcaggtcatt tcttgccagg tcaaccaaca tgcaatgctc cgaattctcc
      601 tttgggtttt gtagcaaatt ggcagcaagt gctttatcca tatctgcatt acctgtcctt
      661 actgccgtac cggcaatagg gttaatggtt gcaatgccgc tatttacctt taggtgagcc
      721 tctggtgacg agccaaaaat tctatagtta aggtagtcga ggtagaaaag gtagggggaa
      781 ggatttatgg agcgtagcgc cctgtataca ttaaaatcgt ctccactaaa acccctgctg
      841 aattgtctag ccagcactat ttgaaacacg ttcccacgtg cacaatgtaa ttttccttgc
      901 tcaaccatag ccataaactg ctcatcgctc atattcgact cttcctcagc ggcggccaca
      961 aatgggaaaa caggcgcatt cctgttggcc agaagcgaaa gcaccctgtc tcctatagca
     1021 ttcatccccg gtgcagtata ttcaacaagc gtcagggtgt tgttaaaatg gttgaaagct
     1081 agaataacct ggtagaaatc ataccttaac aagggaattg aatcgtcaag ggtattaata
     1141 ttgacgctgt caaaaaagtt gacggcatca taggccgtaa acccataaaa tccggctgtt
     1201 tccggatgga tgtttgaacc ctcaagagta aaacttttaa aaaaggagtc aacgaaagcg
     1261 ataatttctg catccttaag cgtatgttcc tcccgttgtt ggtttataaa gtaagtataa
     1321 gtgcggcttt tgccaacctc gatcccggca aatggctgca ggcatatgta ggaaaaggct
     1381 tcactcctgg tgctgtagtc tgaacactca agcagaaggc attgtggaaa aatatctcgc
     1441 agcctaaggt aaatgccgat tggcgtaaac gtatcggctt gcatttccac cgatttaatc
     1501 attaagggta ctgttcgcat agctattgaa atttgtaaaa tttgaaaaat aaaaaagccc
     1561 gctgtgttaa gcgggcctcc attaattctt cctactggtt tacattacgc actcaggggt
     1621 acaatggcct taacacagcg acataacttg ctgcaccacc accaacactg aatgctattt
     1681 atgattatca tatagtcata acttttacca ggtgcaatat taagtacttt tgctattcga
     1741 agcaattatt tttaaatttt ttttcaaaaa aaatacattt catagttttt tgacttatac
     1801 ttgcaaaaaa taggtgaaca tgaaaggttt ctgcacatat ctctatgccg gtttttactt
     1861 ctacttttac tgcaaggtag ggtctggttg gcgtatgtgc tgagctaaag cataacatcc
     1921 acgaaatcag accccgagta gcatcggggt tttttattag ccgtttaggt gaaaaagtaa
     1981 acggttgcta cataaagaaa ttagggcacg aaggactaag actaatttat agggaaatgg
     2041 ccgcaaataa ttctatattc tccttttact acttctacta cttcaaccac attaaccccg
     2101 gggccaggtg catattttag aaatctaacg cataacacaa aactgaaggc cccggaaacg
     2161 gggctttttt tatgaatgta tgatttaaaa attgaaagca atgtaccaaa cggtagcaat
     2221 tcagggcatt aagggatcgt tccatcacca ggctgcaagg gaatgctttg ggttagattg
     2281 tacagttact gagtgcctca catttacaga tcttgttgaa accgtattaa gcggagtggc
     2341 cggatacggc ataatggctg ttgagaattc tttggttgga gccatcctcc ctaactatgg
     2401 gctaatcaga gagtcgtcac tgaccataat tggtgaggtt tacctaagaa ttgaacaaaa
     2461 cctgctagcc ctacccggtc aatcggttga tgaaataaag gaggtgtatt cacatccaat
     2521 ggcattgcag cagtgcgggg tattcctttc catgttgaaa gatgtaagag tagtagcttc
     2581 tgacgacaca gcgctcagtg caaaacaggt tgccgaaaag gggcaaaaag gcgtggctgc
     2641 tattgccagc atggatgctg cgcgagaata tggcctcgag gtagttcagt cggggattga
     2701 atcctataag gataatttca cccgttttgt tgcgcttacc caaaatccga acgtggcatg
     2761 tagccatatg gccaacaagg ccagtatagc atttgccttg ccccctaagc cgggtagcct
     2821 acagaaggca cttcaggtat tctcacgata cggaatgaat ttaaccatgc tgcactccct
     2881 gccccaggtt gggcaatgct gggagtactt cttccatgcc gatttggtgt ttaggcatat
     2941 agacgaccta aaccttgggc tacagtctct taacagatgc accaccttta ccaaggttat
     3001 gggcatttac cactcggaaa gtttgaacaa caactctaca acaacttaaa attatccggt
     3061 atggacttac aaaagaaatg cctggcaatt gttggaacag gccaaatagg ctgctctatt
     3121 ggaatggcag ttaaaaaaca tgtcgcatta gtgacaggga ccgatttaaa ccagaagcac
     3181 ttgcaggaag cggtgcagct ggggttggtt gatgtggcgc ttacccttcg cgaagctata
     3241 gcctatgcaa gcattattgt cgtagcaact cctgtggatg ttgccattcc gctgctagca
     3301 caaattctcg atttgattga cagcaatacc ttagtagttg atgttggctc tacaaaattg
     3361 caagtttgca acgctctaag gcaacatata aatagacgaa actatgttgc ggcccaccca
     3421 atggccggtt catcggttca aggcccaaag ggtgcgctgc caaagctgtt tgatggtgca
     3481 aaaacattta tctgcgaacc ccagctatca tcggattatg cgctagatac ggcaaaagag
     3541 ctatttcatt gcctgaactc aagggtcgaa tttattgatc ccgatgagca cgacaggctg
     3601 gtaggcctgg tgtcgcatct gccgcaggcg ctagcctact cactatgcaa cgtggtgaaa
     3661 ttattcgatg ccgagggatt tgactggaaa ggagcagcag ccaccgcttt tgactcgacc
     3721 acacgcctgg ctgggagctc ttcgaacgta tggctgccta tcatgaatca aaatagggag
     3781 aacctgctga agtacatcac cgcaacggtt ggtgagctgc acaaacttca gcaatacctt
     3841 gagtcaaaca acatacctgc gttggaaacc tacatagaca tagctaacca ggtgaggaac
     3901 cagcttgaag aatcaaaaaa aacaccaaca agtaatagca atggaaaaag aagcacttca
     3961 acagcaaccg ctgcctgaca gctggactat tagtacggtc aagcccttga ttattgcagg
     4021 gccatgtagc gctgaatcac ccgagcagct ccgctgcgtt gcacaggagc tgaaaagaaa
     4081 cagttctgtt tcctacatgc gggcaggtgt atggaagccc cgtacagacc ccaattcctt
     4141 tcagggatat ggagccgaag ccttggaatg gcttcgctcg gttaagcagg aggtcggaat
     4201 gcgcttcgcc accgaggtgg gctgcagcca gcatgtcctc gaagccttaa aatacggcgt
     4261 ggatttggtt tggattggcg cccgaacggt tgccaaccca tttgccatgc aggaaattgc
     4321 tgatgcgcta aaaggtgtag atatccccgt aatggttaaa aacccgctaa atccggacat
     4381 agaactctgg gacggggcaa tctgcaggct ccttaatgcc ggcattcgca aggtaggagc
     4441 aattcatcgg ggtttctcct taataaagaa gtctgtattc agaaatcctc cattatggga
     4501 aattccatta cagctcaagc agcgctatcc aaacatacct ataatttgcg atccaagcca
     4561 catcaccggt agcagaaatc ttgttccgct ggttgcccaa agggctaaag atttgaactt
     4621 tgatggtttc atggttgagg ttcaccccta ccccgatata gcacttagcg accccgccca
     4681 gcagcttact ccccaacaat tcgacaattt gctggaaatg ctattaaaca ataacacacc
     4741 gggttgctca aactcgctgg acatgcttga tgaactaaga gccgaaattg acgttatgga
     4801 cgatttgctg gtctggattt tatcgggcag aatggagctg gccggaaaaa ttgcagggat
     4861 taagaaaatg aagaatttac aaattttgca agcagaacgt tggaaccaga ttcaggaaag
     4921 gttactggcc aaaacttcgg agtcgggcct caggccacaa tttatcagta gcctatatca
     4981 taccatccac aaggaatcgc ttagcattca aagtagcctg atgaaggata agggaaaaaa
     5041 catcaatcac tctcactaaa ctcaagtaaa tgttactacc ctttttgttt ggtttgaatt
     5101 gtgtagattt gcataaacaa gcagatggaa gctatagctg ttaaggataa aacaagcatt
     5161 tttgtggggg aaagtatgga caacctttca agctacattc cccgagaggg tgtctttatt
     5221 attaccgaca gcaacattga caggatttac ggcaacaggt t
//
LOCUS       DAOSHM010000110         5106 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_14535_length_5106_cov_7.111265, whole genome shotgun sequence.
ACCESSION   DAOSHM010000110 DAOSHM010000000
VERSION     DAOSHM010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 5106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5106
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14535_length_5106_cov_7.111265"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2071
                     /locus_tag="PK839_10305"
     CDS             <1..2071
                     /locus_tag="PK839_10305"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HOG73167.1"
                     /translation="GKEFFRVDAQARTKVPAFDQEKLALLLADSLKRDVKPYELPISK
                     CEPTGENEVVFWAGERQWVYRTDTNLLLPKSNEPQLSPTESLSPDGQWIGYINDGNLF
                     LKNRRLKTETQLTSDGTPENGYGCSLDWYFTRNDSKGEQNEYSIEVYWSMDSKKLIVP
                     RYDRRNVRRLQLMKYDDVEGFQSELISYERALAGDTVLTMTDFYIFDVESSKMVKVDL
                     PSNPAFLGTYIYTFEGCPKAYHVRYYRGYKTSELFEIDLETGRVRSVLAESYPKTFVD
                     IYTETLEVMYDRNEFIWRSEMDGWSHLYRYNLSTGQPVNQITRGSFYVYGLEDTDSKT
                     GTLWFTAGGMDSSRNPYQKYLYSINKNGKNLKLLTPEEANHDIRVSPDKKYFVDNFSS
                     PTRPNTTVVRRLADGKQLMEIEKSDITDLINMGWKPAEQFSLAADDAKTPLYGLIIKP
                     TNFDSTKKYPVIDATYTGPHTISTPQTFSSSVLNMNLSLAELGFVVVYIDGRGSAYRS
                     KAFHDVSYARLGFGLVDHVYAIKKLAQKCAYLDTNRVGIYGHSAGGYDATRGLLLFPE
                     FYKVAVSSAGDHDHRMEKEWWPELYQGFPVDTQYQNQSNITNAGKLKGHLLLATGDLD
                     NNVNPSATIKLAEELTKANKDFELLILPNEDHGGCFWNKYFIRKRWDFFVKHLLSQEP
                     PREYKIQ"
     gene            2164..3552
                     /locus_tag="PK839_10310"
     CDS             2164..3552
                     /locus_tag="PK839_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HOG73168.1"
                     /translation="MFSKQTYTNRRNQLRAQFKSGILLFIGNDESGMNYEDNTYRFRQ
                     DSSFLYYFGINRPGLYAIMDIESGHDYVYGDDLTIDSIVWMGSQPTIHEMAAGFGVEN
                     TGPLSSFYQKVKTYPLASVHYLPPYRPEHFLKLMDIFGISKQEASARASVPFITAVAE
                     QRNVKTDEEVEEIDHAVSITAEMHMAAMRYALPGMTEAQVAARIYEVALAAGGDISFP
                     IIATINGQTLHNHYHGNTISEGQLFLVDAGYETPKGYAGDMSSTFPVGRTFNERQKEI
                     YQITLAAHNGAIDKLKPGIHFRDVHLHAARIIFDGLKNMGFAKGNTDDAIANGAHALF
                     FPCGVGHLMGLDVHDMENLGEQYVGYCGAPKSTQFGLKSLRLARPLKPGFVVTIEPGI
                     YFIPELIDLWQQNGTNRDYINFDRVNEYRHFGGIRNEEDVLVTPTGHRVLGKPLAKTV
                     EEVEALRAEAFF"
     gene            3941..4435
                     /locus_tag="PK839_10315"
     CDS             3941..4435
                     /locus_tag="PK839_10315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG73169.1"
                     /translation="MTNKLLVAILFLCVFIQNSFSQNNDQINDLYLKIKKIEDRESDI
                     SKMKSRRGVSDRKYLIKSIIDSGEIISKHKIRYYRSGIKKERIKYYKKSKNEKILVLD
                     IIKINELKTYIKYCETDLNYKKEMIVVKEEVLIDNRYYRINTFDKYGVLTKTDNIFIS
                     SKDK"
BASE COUNT         1561 a         1104 c         1170 g         1271 t
ORIGIN      
        1 agggaaggag tttttcaggg ttgatgcgca agctcgtaca aaagtcccgg cattcgacca
       61 ggaaaagctg gcattgctac tggccgacag cctgaaaagg gatgtgaaac cctacgagct
      121 gcccatatca aagtgtgagc ctacgggtga aaacgaggtg gttttttggg ccggagaaag
      181 gcaatgggtt taccggacgg ataccaattt gctgcttcca aaaagcaacg aacctcaact
      241 ttccccaaca gaatcgctat cacccgatgg ccaatggata ggatacatta atgatggcaa
      301 tctttttttg aagaaccgcc ggttgaaaac cgaaacccag ctaaccagcg atggaacgcc
      361 cgagaatggt tacggatgca gcctggattg gtacttcacc cggaacgaca gcaagggaga
      421 gcaaaatgaa tactccattg aggtttactg gagtatggat tctaaaaagt taatcgttcc
      481 ccgttacgac aggcgtaatg ttcgcaggtt gcagctgatg aagtatgatg atgttgaggg
      541 gttccaatcc gagctgattt cctacgaaag ggctctggcg ggcgatacgg ttttaaccat
      601 gactgatttc tacatttttg atgttgaaag ttctaaaatg gttaaggttg acctgccttc
      661 aaaccccgca tttctgggaa cgtatatcta cacctttgag gggtgcccaa aagcttacca
      721 tgtaaggtac tacagggggt ataaaaccag cgagctattc gaaattgacc ttgaaacggg
      781 gagggtacgc tcggttttgg cagaaagcta ccctaaaacc tttgtggata tttacaccga
      841 aacgcttgag gtgatgtatg acaggaacga gtttatttgg cgctccgaaa tggatgggtg
      901 gagccattta taccggtaca acctttcaac aggacaacct gtaaaccaaa taaccagagg
      961 tagcttctac gtttatggtt tggaggatac cgacagtaaa accgggacgc tttggttcac
     1021 cgccggcgga atggatagta gcaggaatcc ctaccagaag tacctgtact ccatcaacaa
     1081 aaatggtaaa aaccttaagc tgcttacccc cgaggaggcc aaccacgaca ttcgcgtatc
     1141 acccgataag aagtacttcg ttgataactt ctcgtcgccc acgaggccaa acaccacggt
     1201 ggttcgaagg ttggctgatg ggaagcagct gatggaaatc gaaaaatccg atatcaccga
     1261 cctgataaac atggggtgga agcccgcaga acagttcagc ttggctgccg acgatgcgaa
     1321 aacaccgctt tacggactaa tcatcaagcc aacgaatttc gattcaacca aaaaataccc
     1381 cgttatcgac gcaacctata ccggtcctca caccatcagc actccccaaa cattctcgag
     1441 cagcgtgtta aacatgaacc tgtcgttggc agagctgggt tttgtagtgg tatatattga
     1501 cgggcgtgga agcgcatacc gctccaaggc atttcacgat gtgtcctacg cacggctagg
     1561 ctttggtttg gttgaccatg tttacgctat taaaaaactt gcgcaaaaat gtgcctacct
     1621 cgacaccaat agggtaggaa tatacggcca ttcggccgga ggctacgatg ccacacgagg
     1681 attgctccta tttccggaat tttacaaggt ggctgtttcg tcggctggcg atcacgacca
     1741 tcgtatggaa aaagagtggt ggccagagct ctaccagggt tttccggttg acacccagta
     1801 tcaaaaccag tcgaacatta ccaatgccgg caagctaaag gggcatttgc tgctggcaac
     1861 cggcgatttg gataacaacg taaacccttc tgcaaccatt aagcttgccg aggagctaac
     1921 caaagccaat aaggactttg agctgctaat ccttcctaat gaagaccacg gtggctgttt
     1981 ctggaacaag tactttatca ggaaaagatg ggatttcttt gtgaagcacc tacttagcca
     2041 agagccgcca agggagtata aaattcagta acctgttata catccacccc gggcggagca
     2101 ggagattaca aatttcgacc cagcctgtga gtgtaataat aaccaactaa aaattcaacc
     2161 agcatgttca gcaagcaaac ctataccaac cgtcgcaacc agctgcgggc acaatttaaa
     2221 tccggtatcc tacttttcat tggaaatgat gagagcggaa tgaactatga ggacaacacc
     2281 taccgttttc gtcaggatag ctcatttctt tactattttg gcattaacag gccaggcctt
     2341 tacgctataa tggatataga gagtgggcac gactatgttt atggcgacga tttaaccatc
     2401 gacagcattg tgtggatggg gtcgcaacct accattcacg aaatggctgc tggatttggc
     2461 gttgaaaata ccggcccttt atccagcttt taccagaagg taaaaaccta ccctttagct
     2521 tccgtccact accttccgcc ctaccgtccc gagcacttcc tgaagctgat ggatattttt
     2581 ggaatcagca aacaggaggc atcggcaagg gcaagcgttc catttattac agccgttgcc
     2641 gagcagcgaa acgttaaaac cgacgaagag gttgaggaaa ttgaccatgc cgtatccatt
     2701 acagccgaga tgcacatggc ggccatgcgc tatgcccttc cgggaatgac agaggctcag
     2761 gttgctgcca gaatttacga agtagccctt gcagctggcg gagatatcag ctttccgata
     2821 atagcaacta ttaacgggca aacgctgcat aaccactacc atggtaacac cataagtgaa
     2881 ggccagcttt tccttgttga tgccgggtat gaaactccta agggttatgc gggcgatatg
     2941 agcagcactt tccctgttgg aagaaccttc aacgagaggc aaaaggaaat ttaccaaata
     3001 acccttgccg ctcacaatgg agccattgac aagctaaaac caggtatcca tttcagagat
     3061 gtacacctac acgcagcccg tatcattttt gatggcctaa agaatatggg ctttgcaaag
     3121 ggtaacaccg atgatgccat tgcaaatgga gcacatgcac ttttctttcc atgtggggtt
     3181 ggccacctga tggggctcga tgttcacgat atggaaaacc tgggtgagca gtatgttggc
     3241 tactgtggtg ctcctaaaag cacgcaattc gggttaaaat cgttgaggct tgcacgccct
     3301 cttaaacccg ggtttgtggt aaccattgaa cccggcatct actttatccc tgagcttatt
     3361 gacctatggc agcaaaatgg gacaaacagg gactacatca acttcgacag ggtaaacgag
     3421 tataggcact tcggaggaat tcggaacgaa gaggacgtgc tggtcacccc caccggccac
     3481 agggtgctag gcaaaccctt ggccaagacg gttgaggaag tggaagccct cagggcagag
     3541 gcattttttt aaaaaggatt acccgaagcc ttgggggaca taacctcccc gagcaggcat
     3601 ataacccgca ggtacctggt aagggtaccg ctgtaaaatc tgtagggcca accgttccga
     3661 atacctgaac gcagctggcc acctgcggtt ttatccggtt gctacccggc cataaaagtg
     3721 gtcgggctac agatgtttaa acccaggcgt gccagaaaaa tttacaggaa attagaagcc
     3781 catttgatga gttgtaaaat aaattctaaa tttgtttata gaaatagaac gcaactttat
     3841 ggcgtctata catccaaata tggaaataag ggcgctatgg agtggcacct ataaacgagt
     3901 tatagaccag ttaaattgac ataacaaaaa ctattacaat atgacaaaca aacttttagt
     3961 tgctatttta tttctctgtg tttttattca aaattcattt tctcagaata acgatcagat
     4021 taacgacttg tatcttaaaa taaagaaaat tgaggacaga gaatctgata tttctaaaat
     4081 gaagagtaga agaggtgttt cagaccggaa atatttgatt aaatccataa ttgactcagg
     4141 agaaattatt tcaaagcaca aaataagata ctatcgtagc ggcataaaaa aagagagaat
     4201 taaatattat aaaaaatcaa aaaatgaaaa gattttagtt ttagatatta ttaaaataaa
     4261 tgaacttaaa acttacatta aatactgtga aacagactta aactacaaaa aagaaatgat
     4321 tgtagtaaaa gaagaagtat taatagataa caggtattac agaataaaca cttttgataa
     4381 atatggagtt ttgacaaaaa ctgacaatat atttatttca tcaaaagata aataaccggc
     4441 ctataacacg tggcaaataa tattggggct agaccatgca aatacggggt ttttattacc
     4501 tttaacgtgg tttttaacgg cggatagatg ttcggtttct aactcaccat catttcttgc
     4561 cacggaaacg ttgtgtgtaa tgcaattgac gatatgaaac gattactgtt ttaagtatta
     4621 tttttccaga caacatttct ttttttctcc gctaccgcac gcgtcctctc gtgtggtagt
     4681 ataaaaaaag tagtgggtag gaattagtaa aaggataatc cgaggttgcc acacgacagg
     4741 agtcgtgcgg cagcgatgag tagttggaag gtcgccgaag aatagaaccg acccaccacc
     4801 cggcggggag ttttgcgaag gcaaccgtgc ggctacaatg ggaaccgagc ttgcgagctc
     4861 aacgaagtta atttttagaa cccaccttaa tcaaaacatt cgtaaacgca actaattttt
     4921 cttaaatagt tattaactta acataatgat accatgtatt ttatctttca atatcaagca
     4981 taaacccaaa agtggtttac gcaagtttta aattttgcat ttacgaatgt tgtatattag
     5041 ttgcgtaaat aagacgttgt gcgtcagctt aaaagacaag accccgataa tggaaatacc
     5101 acctta
//
LOCUS       DAOSHM010000111         4882 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_15446_length_4882_cov_21.162420, whole genome shotgun
            sequence.
ACCESSION   DAOSHM010000111 DAOSHM010000000
VERSION     DAOSHM010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 4882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4882
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15446_length_4882_cov_21.162420"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            215..4822
                     /locus_tag="PK839_10320"
     CDS             215..4822
                     /locus_tag="PK839_10320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG73170.1"
                     /translation="MSRRPARKPRIAATLGLILLALLSLRINALHQDEGKFSGNPLLF
                     PTEVKTIIPDLGKDDEAYLKAFSIFWLSDSIPEDQKGNIIETANLILPILPESKAIVL
                     DYIKLAATFYRDSYANKNYAPWNGALKQLLLTENLDYNKLLAFFRFTRNTVDSSFLRQ
                     TISYTWKVSPHHSYNFEFSSNQLLARFTNVNLVCITGTDSIYIEGTSGYYNPENDMWV
                     GSKGKVTWVRSGYPKDAIFAMLSSYRFPLSKNSYTVDSVYFTNKDYFDRPARGTLTDK
                     LVKDYKPTTIDYPEFKSYDNWFKIKNLFERVDYEGGYIMRGSRLLGVGSGEQLAKVSI
                     SRNGKPFLKVEGNIMIFERSILTADRASILFRFEEDSIYHNGLYFSYNNNTRTVTILP
                     TERITSQSPFYSSYHKLSIWTDQLSWNIDGDKLIFGASRGSSIGNASFESNNFFNEVY
                     FDQLMGPSDRHPLLEVWNFSVRNKSKTFLATDLAQKLRKPVEEVKIEMMRMAKLGYVL
                     YDFETDEVQVTEKLKHTVMAKFQKVDYDVIRFSSTCPQSQPNAELDIASMNMKINGVN
                     NISVSDSQNVFINPYNQQVIMQKNRNFEFAGEVQAGLFRFYGSKYRFNYDEFKIDLLQ
                     VDSLKLDYQTRYRDGYGRKILQSVANTFHIIGGDIVIDKPSNKSGLQSNPDYPIFHST
                     QKSYVYYDDPSIFNGIYKRDSFYFEIQPFTYVNMDNFEQSDLDFKGILYSRNILAPIE
                     ENLVLRPDNSLGFIKSTPPEGLALYRGKGKVFDKIDLSNRGLTVKGQVGYITSTTRSD
                     DIFLFPDSMVTSAASFTMEKQVAGVEFPNVTGDLHHVKWLPQNDKLFVTKGEKPFKMY
                     DSLGIFSGDLLLQPLGLTGIGSMSIPNAALSASLFEFNADDFTTPVSSLTLYSPANQN
                     VALTTKDVRSKISFKSQTGEFSKTNQSLFATLDELGYEAYLDRFSWNMVTNNLAMQTL
                     MQQQDVKDGKFRVSNMVEGDSIPKGSLFYSVKSSEDSLYFFSAKSTYNTATAELNADS
                     VSYIVVADATIMPKDKKIRVNKPNRMLPLSEATIKANNLTAYHRFFDADVSIISRNSY
                     SGKGHYYYADENDSLQTIYFTQIKVDSARNTVATGTITEPDSFTLSPQFAFVGQVHLS
                     APQKLLYFEGGAKPSYQCTGTTGQWIKFEALIDPRRVLIPITEQPKNLNLHPLISGSV
                     ISEDSIKVYGGLLRNRKNYDDKPLVNASGYLSFNNNNRRFTIAPLHKLSNPDTSGSSV
                     SLQKDFCMVFSDGLVKLPVNLGQVKLNAAGSTTHKLEGNDLTLDLVLQLNFHFNQKSL
                     EAIALELNQSFALEKVDLSRKLYRKALYQLVDSSELHAAISQLDLFGSFSQIPKGYES
                     TITFSDLKMRWNPLTKSFVSKGKLGIGTIGNIQVNRFVNGYIEIFKRRSGDLMTLYLD
                     FGEDRYYVFTYTKSVMQVSSNNPEFVQPIEQQKSSETRLKVKPGEPGYRYLIGTKKAL
                     DQAQQRYNQLMSGDEDKVAPPEDEVVEEGRDKVKEKGEKRN"
BASE COUNT         1427 a         1202 c         1068 g         1185 t
ORIGIN      
        1 ttaagaaata accaacaagt catcgtccgt actacccttg acaaattcag aacttgcgct
       61 acctttgctt aaaaaggatg aaagggataa tgtcccaaac cgtgttgagt aatgtgtcca
      121 aagagcaggg agttaaacct ttagcagcct gtaaaatagg tttattacca ttcggaagct
      181 gaatggcagt actaacttaa tctcatcctc cgtcatgagc cgcagaccag ccagaaagcc
      241 tcgaatagca gctacacttg ggttgatatt gctggcgctg ctgtcgctaa gaataaacgc
      301 cctgcatcag gacgaaggga aattctcggg caacccgctc ctcttcccca ccgaggtaaa
      361 aaccataatt cccgatttgg gtaaggatga cgaggcctac ctgaaggctt tcagcatttt
      421 ctggctttcc gattccattc ccgaagacca gaagggaaat attatcgaaa cggccaacct
      481 aatactaccc attctacccg agagcaaggc tatcgttctg gattacatca agttggctgc
      541 tacattctac agggatagct atgctaacaa aaactatgca ccctggaatg gcgcgctaaa
      601 acagctgctg cttaccgaaa accttgacta caacaagttg ctggctttct tcaggtttac
      661 ccgaaacacc gttgacagct cgttcttaag gcaaaccata agctacacgt ggaaggtaag
      721 tccccatcat tcctacaatt ttgaatttag cagtaaccag ctgctagcca ggtttaccaa
      781 cgtcaaccta gtatgcatta caggtaccga tagcatatac attgaaggaa ccagtggtta
      841 ctacaatccc gaaaacgaca tgtgggttgg aagtaagggt aaagttacct gggtacgctc
      901 gggctacccg aaggatgcca tttttgcgat gctgtcgagc taccggtttc ccctttccaa
      961 aaattcctac acggtggact cggtttactt caccaacaag gactacttcg accgtccggc
     1021 acgcggcacc ctcaccgaca agctggtaaa ggattataaa ccgaccacaa ttgattaccc
     1081 cgagtttaaa tcgtacgata actggtttaa aattaaaaac ctgttcgagc gggttgacta
     1141 cgaaggaggt tacatcatga gggggtcgcg gctgcttggc gtgggttctg gtgagcagct
     1201 ggcaaaggta tcaatcagcc gaaacggcaa accgttcctg aaagtggaag ggaatattat
     1261 gatttttgaa cggtcaatcc tcaccgccga cagagccagc attcttttca ggttcgagga
     1321 ggactcaatt taccataacg gcctttactt cagctacaac aacaacaccc gaacggtaac
     1381 cattttaccc accgaacgca tcacctcgca aagccctttc tatagctctt accacaagct
     1441 gagcatttgg accgaccagc tttcatggaa tattgatggc gataagctga tttttggcgc
     1501 ctcgagggga tcaagcatag ggaatgccag ctttgaatcc aataattttt tcaatgaagt
     1561 ttacttcgac cagcttatgg gccccagcga ccgtcaccct ctactggagg tttggaactt
     1621 ttccgtcagg aacaagagca aaacctttct ggccaccgat ctggcccaaa agctgcgcaa
     1681 gccggtggag gaggttaaaa ttgaaatgat gcgaatggcc aagctggggt acgtgctcta
     1741 cgattttgaa accgatgagg ttcaggttac cgaaaaacta aagcatacgg tgatggcgaa
     1801 gtttcaaaag gttgattacg acgtgattcg cttctcatca acctgtcctc aaagccaacc
     1861 caacgccgag cttgatattg ccagtatgaa catgaagatt aacggcgtaa ataacatttc
     1921 ggtgagcgat tcgcaaaacg tattcatcaa cccatacaac cagcaagtca tcatgcagaa
     1981 aaaccgtaac tttgagttcg ccggagaagt gcaagccggc ttattcaggt tctacggctc
     2041 gaaataccgc ttcaactacg acgagtttaa gatcgacctc ctacaggttg actcgctgaa
     2101 acttgactac caaacccgtt accgagatgg ctatggccgt aaaatcctgc aaagtgtagc
     2161 caacaccttt cacatcattg gtggcgacat tgtaattgat aagcccagca acaagtccgg
     2221 actccagagt aatcctgact accccatttt tcatagtact caaaaatcat acgtttacta
     2281 tgacgacccc tcaatcttta acggcatata caagcgtgat tcgttctact tcgaaataca
     2341 acccttcacc tacgtgaata tggataattt tgagcagtct gacctggact ttaagggtat
     2401 tctgtattcc cgcaacatcc ttgcccccat tgaggaaaac ctggtgcttc gacccgataa
     2461 ctcgctgggt ttcattaagt caactccacc cgagggttta gcgctctacc gtggaaaagg
     2521 gaaagttttt gataaaattg acctgagcaa cagggggctt actgtaaaag gacaggttgg
     2581 ctacattacc tctaccaccc ggtcggacga tattttcctt ttccccgatt ccatggttac
     2641 cagtgctgcg tcattcacaa tggaaaagca ggttgctgga gttgagttcc caaatgttac
     2701 aggcgacctt caccatgtaa aatggctacc ccaaaatgat aagcttttcg ttacaaaggg
     2761 ggaaaaaccc tttaaaatgt acgattccct gggcatattt agcggcgact tgctactcca
     2821 accattaggc cttactggaa taggaagtat gagcattcca aacgcagctc tttcggccag
     2881 cctatttgag ttcaatgccg acgattttac cacgccggta tcgagcctaa ccctgtactc
     2941 accagctaac cagaatgttg cgcttactac aaaggatgta aggtcgaaaa tcagctttaa
     3001 aagtcaaaca ggagagttct ccaaaacaaa ccaaagcctt tttgccacgc ttgatgagtt
     3061 gggatacgag gcctaccttg accgttttag ctggaatatg gttaccaaca acctggctat
     3121 gcaaaccctg atgcaacagc aggatgtgaa ggacggtaaa tttagggttt ccaacatggt
     3181 tgaaggtgat tccatcccaa aggggtctct cttctactca gtaaaaagca gcgaagactc
     3241 gctttacttt ttttcggcaa aatcaacgta taatacagca acagctgaac ttaacgccga
     3301 tagtgttagc tatatcgtgg tagccgatgc caccattatg ccaaaggata aaaaaatcag
     3361 ggtgaacaag ccgaaccgca tgcttcccct ttccgaagcc accatcaagg ccaataacct
     3421 tacggcctac caccggttct tcgatgccga tgtatctatt atcagccgaa acagctatag
     3481 cggaaaaggt cactactact acgctgacga aaacgattcg cttcaaacta tttactttac
     3541 ccagataaaa gtggatagcg ctaggaacac agttgctacc ggaactataa ccgaaccaga
     3601 cagcttcacg cttagcccac agtttgcctt tgttggacag gtacacctct ccgcgcccca
     3661 aaagctactc tatttcgaag gaggagccaa accatcatac caatgcacgg gaacaacagg
     3721 ccagtggata aaatttgaag cgttgattga tcccaggaga gttcttatcc ccataaccga
     3781 acagcccaaa aacctcaacc tccatccgtt aatcagcggg tcggtaattt cggaggattc
     3841 gataaaagta tatggcggac ttctgcgtaa caggaaaaac tacgatgaca agccgctggt
     3901 taatgcttcg ggatacctga gctttaacaa caataaccgt agatttacca ttgctccgct
     3961 acacaagcta agcaatcccg atacatcggg tagtagcgtt agcctacaaa aggatttctg
     4021 catggttttt agcgatggcc tggttaagct acctgtcaac ctcggtcagg ttaagcttaa
     4081 tgctgcaggc agcactactc ataagctaga aggaaacgat ctaacccttg accttgtgct
     4141 acagctaaac ttccacttta atcagaaatc acttgaggct attgccctag agctaaacca
     4201 gtcgtttgcg ctcgaaaagg ttgatctttc aagaaaactc taccgaaaag cgctttacca
     4261 gctagttgat tcatccgagc tgcatgctgc catcagccag ctggatttat ttggaagttt
     4321 tagccagatt cccaagggat acgaaagtac aatcaccttt tcagacttaa agatgagatg
     4381 gaacccctta accaaatcgt ttgtttccaa gggaaagttg ggcatcggca caattggcaa
     4441 tattcaggtt aaccgttttg tgaatggtta catcgaaatc tttaaacgcc ggtcgggcga
     4501 cctgatgacc ctttacctgg actttggcga agataggtac tacgttttta cctatacaaa
     4561 aagcgtgatg caggtttcat cgaacaatcc cgagtttgtt cagcccattg aacagcaaaa
     4621 atcgagcgag accaggctaa aggttaaacc cggcgagcca gggtaccgtt acctcatagg
     4681 tacaaaaaaa gctctggacc aggcccaaca gcgttacaac cagctcatgt ccggtgatga
     4741 ggacaaggtg gcacctcccg aggatgaagt agttgaggaa ggaagggata aggtaaagga
     4801 gaaaggagaa aagagaaatt aaacttatcc gcctaccggc ggaacttgtc cggctcctga
     4861 cggaacttgt ccgcctactg gc
//
LOCUS       DAOSHM010000112         4870 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_15495_length_4870_cov_19.061475, whole genome shotgun
            sequence.
ACCESSION   DAOSHM010000112 DAOSHM010000000
VERSION     DAOSHM010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 4870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4870
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15495_length_4870_cov_19.061475"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..944
                     /gene="aroB"
                     /locus_tag="PK839_10325"
     CDS             <1..944
                     /gene="aroB"
                     /locus_tag="PK839_10325"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HOG73171.1"
                     /translation="GVFIITDSNIDRIYGNRLPPLPKFVANPGEQSKTLLRLTEIYRW
                     LLDSGADRNSFIVGIGGGVVCDITGFVASTYMRGVRFGFVATSLLAQVDASIGGKNGV
                     NLDGYKNIVGTFNQPQFVICDTSLLKTLPETELSNGFAEMVKHALIADAPQFTILESN
                     TNRLNTCDLELLDSLVASSVGVKASIVTADERESGERKKLNFGHTWGHAVEKVTGIPH
                     GQAVSIGMAFASQLSAEKGLLTPDECHRITSLLIALNLPVSIKANTNNIFEALQRDKK
                     RCSNAIDFILLEGIGKAVIKSLSLVEIKEFIERKYCY"
     gene            1020..1676
                     /locus_tag="PK839_10330"
     CDS             1020..1676
                     /locus_tag="PK839_10330"
                     /inference="COORDINATES: protein motif:HMM:NF013640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HOG73172.1"
                     /translation="MKILHPDICLSIGNMRYEDIFPYLKVAALAEIRLDLLNLTDEQI
                     RGLFTAHGNLIATYRCNDSAKMFQTLTNTLDSGAAFVDVDCLTENSIRDAIVAKAKGL
                     NRRVIISYHNFECTPNKEELERIIYSMKSQGADICKVACMANEPLDCSRVMALYEHIP
                     DLVAFCMGEAGKITRLAAPILGAPFTYASVAGASTAPGQLDYLKMEEYLNYFLPLNHD
                     "
     gene            1669..2604
                     /locus_tag="PK839_10335"
     CDS             1669..2604
                     /locus_tag="PK839_10335"
                     /inference="COORDINATES: protein motif:HMM:NF020090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOG73173.1"
                     /translation="MTRKVYAVFGNPVLHSCSPQLFNSVFSEIPVEAYYTRIRPANAA
                     NIPELIRHLNLAGANITTPFKTEVMTHLDWLSDEAEIIGGVNTIINNQGYLKGYNTDY
                     LGVSRSLQEAGLHLQGSRCLVLGAGPAACAAVYALINEGADVTVVNRTNHKAKEIANR
                     FGCSSADMDKLDSAILECDILVSALLPEANPLANIKVPEWITAIDANYRASTLSQHLA
                     LSGCRTITGKRWLLHQAAASFNLFLNFNPKIELMAKGLEAQPQREAARYQMIGEDTLR
                     SIHTEDFDMLVSVPDSSTMTFRTILDEEISKAFVG"
     gene            2573..3829
                     /gene="aroA"
                     /locus_tag="PK839_10340"
     CDS             2573..3829
                     /gene="aroA"
                     /locus_tag="PK839_10340"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HOG73174.1"
                     /translation="MRRLVKPSLVSGAVQAPPSKSVAQRAIAMATLANGVTEIYNPGN
                     SNDSKAAVEICRALGASIAEYPGKLVVTGGLQQPSQILNCGESGLGIRMFSAIASTFS
                     AGVTLTGTGTLLKRPMQPIADALVQLGAACSTSSGFLPITVKGPINGGVANIDGSQGS
                     QVLTGLLVASPLAKHDVTIRATKLKSVPYVHLTIQMMHDFGVEVDVGSDIDFHVRAKQ
                     RYAPCTYNVEGDWSGTAFMLVAGATAGEVEVKNLNPSSLQADRAIVDALVMAGATVDV
                     EKNGVYVKRNKLSHFEFDATNCPDLFPPLVALASHCDGESKILGVSRLRVKESDRAAT
                     LVEEFAKLGIDIKIKDDLMVVQGGKVKSAWVHSHGDHRIAMACAVAALSGEGNLTIEG
                     AESVSKSYPRFFDDLNSIMTNSSLNV"
     gene            3845..4828
                     /locus_tag="PK839_10345"
     CDS             3845..4828
                     /locus_tag="PK839_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HOG73175.1"
                     /translation="MNSFGRIFRVSLFGESHGRAIGVVVDGVKPGLPLSTDDFADDIR
                     RRGPGLPGTTPRTENDLPEMVSGWFNGYTTGAPLAIIFRNTNVRSDDYERIKDLPRPG
                     HADFVAYKKFSGYNDYRGGGHFSGRITLALVAAGVIAKKMIDPVEVHAKLVTVGGSTD
                     IENTINKAMSANDSVGGLVECRVSGIPIGWGEPFFDSVESVISHLAFAIPGVKGIEFG
                     SGFAAASMFGSAHNDAFIDASGATSSNNAGGILGGISNGNELQFRVAVKPTSSIAREQ
                     QTINMRTGMPEKLVVTGRHDTCIALRVPVVLEAVAAIALADLAAIQNKSKG"
BASE COUNT         1310 a         1137 c         1226 g         1197 t
ORIGIN      
        1 agggtgtctt tattattacc gacagcaaca ttgacaggat ttacggcaac aggttgccgc
       61 cactccctaa atttgtggca aatcccggcg agcagtccaa aacccttctc cgcttgaccg
      121 aaatttaccg gtggttgctc gactctgggg ccgacaggaa ctcgtttatc gtggggattg
      181 gcggcggggt tgtttgcgat attaccggtt ttgtggcctc tacctacatg cggggggtta
      241 ggtttggctt cgtggccacc agcctgctcg cccaggttga cgcaagcatt ggtggtaaga
      301 atggagtgaa tctcgatggc tataagaaca ttgtgggaac attcaaccag ccgcaattcg
      361 tgatttgtga tacctcgctt cttaaaacgt tacccgaaac cgagctgtcc aacggttttg
      421 ccgaaatggt aaaacatgcc ctgattgccg atgctcccca atttaccatc ttggaaagta
      481 acactaatag gcttaacacc tgtgatttag aattactcga ctcgttggtt gcctcatcgg
      541 ttggggttaa ggcatcaatt gtaaccgccg atgagagaga gagcggcgaa cgaaagaagc
      601 ttaactttgg ccacacatgg ggccatgcag ttgaaaaggt aacagggatc ccccacggac
      661 aagccgttag cataggaatg gcattcgcct cgcagctctc ggccgaaaaa gggttgctca
      721 caccagatga atgccatcga ataaccagct tgcttattgc gcttaatcta cctgtttcca
      781 taaaagccaa caccaacaat atttttgagg cattgcagag ggataaaaaa cgatgctcca
      841 acgctataga cttcatcctg cttgagggca ttggaaaggc cgttatcaaa agcctaagcc
      901 ttgttgagat taaggagttt atagaaagga agtattgtta ctgaaaagaa aagttaaaag
      961 ttgactattc ctgcttttta tacaagttgc gacagcatat tactgatgag aaaacggcta
     1021 tgaagatttt gcaccccgat atttgcttga gcataggaaa catgaggtat gaggatattt
     1081 tcccctacct gaaggtagct gcccttgccg aaattcggct cgacctcctg aatcttacag
     1141 atgaacagat acgagggctt tttactgcac atgggaacct gattgcaact tataggtgca
     1201 atgacagcgc taaaatgttt caaacactta ccaatacact cgattcaggt gcggcctttg
     1261 ttgatgttga ctgccttaca gaaaattcca tcagagatgc cattgttgcc aaggctaaag
     1321 ggctaaaccg aagggttatt atctcatacc acaactttga atgcacaccc aacaaggaag
     1381 aactcgaaag gataatttac agcatgaaaa gccagggtgc cgatatttgt aaggttgcat
     1441 gcatggccaa tgaaccgctt gattgctcaa gggtgatggc tttgtacgag cacataccgg
     1501 atttagtagc tttttgtatg ggtgaagcag gaaaaattac ccggctagcc gctccgattc
     1561 tgggcgcacc atttacctac gcttcggttg ccggcgcatc aactgctccc gggcaactcg
     1621 actacttgaa aatggaggaa tacctgaatt attttttacc gttaaaccat gactaggaag
     1681 gtgtatgccg tttttgggaa tcctgtgctc cacagctgca gcccccagct tttcaactcg
     1741 gttttcagcg aaatacccgt tgaagcctac tacacccgta tccgacctgc aaatgcagcc
     1801 aacattcccg agttgataag gcacttgaat ctggctggtg caaacatcac cacgcctttt
     1861 aaaaccgaag taatgacgca cctcgattgg ctttcggacg aagcagaaat cataggtggt
     1921 gtaaatacca taattaacaa ccagggttac ctgaaggggt acaacaccga ctacctgggt
     1981 gtgagtcgtt cgcttcaaga agcaggcctt catcttcagg gtagccgatg ccttgttttg
     2041 ggcgcagggc ctgctgcatg tgctgcagtt tatgccttga ttaatgaggg cgcagacgta
     2101 acggttgtta acagaacaaa ccacaaagca aaagaaatag caaatcggtt tggctgtagc
     2161 tcagctgata tggataaact ggacagcgcc attctcgaat gcgatattct ggtttcggcg
     2221 cttcttcccg aggcaaatcc acttgcaaac attaaggttc cggagtggat tacggcaatt
     2281 gatgccaact accgtgcctc aacgctatca caacacctgg cattaagcgg ctgcagaacg
     2341 attacgggta aacgctggct gctacaccag gcagcagcct cattcaacct ttttctaaat
     2401 ttcaacccga aaattgagct aatggcaaaa ggacttgagg cacaacccca aagggaagct
     2461 gctaggtacc aaatgatagg agaagacaca ctccgttcga ttcatacgga agatttcgac
     2521 atgctcgtat cggttcccga cagctcaacc atgacattca gaacaatttt agatgaggag
     2581 attagtaaag ccttcgttgg ttagcggagc tgtgcaggcc cccccctcga aaagcgttgc
     2641 ccaaagggca atagccatgg caactttagc caatggcgtt acggaaatat acaatcctgg
     2701 caacagtaac gattctaagg cagcagttga gatttgccgg gcgcttggcg ccagcatagc
     2761 tgaatacccc ggtaaactgg tggtaacggg cggattgcag caaccctccc aaatccttaa
     2821 ttgtggcgaa tccggtttgg gtattagaat gttttcggca attgcctcaa catttagtgc
     2881 cggcgttacc ttaaccggaa ccggaacgct cctaaaaagg cctatgcaac ccattgctga
     2941 tgcgctggtt caacttggag ctgcctgcag cacctcatcc ggatttctac ccataaccgt
     3001 gaaaggccca attaatggcg gagtagccaa catagatggc tcgcaaggct cgcaggtgct
     3061 caccgggcta ctcgtggctt caccactggc caaacacgat gttaccataa gggcaacgaa
     3121 actcaaaagt gttccctatg tgcacctcac cattcaaatg atgcacgatt ttggggttga
     3181 ggtagatgtt ggatcggata ttgattttca cgtaagggca aaacagcgtt atgctccctg
     3241 cacctacaac gttgagggtg attggagcgg aactgcattc atgcttgttg ccggtgctac
     3301 ggcaggcgaa gtagaggtga aaaatcttaa cccctcgtcg ctgcaggccg atagggcaat
     3361 tgttgatgcc cttgtgatgg ccggcgctac tgtggatgtt gaaaaaaatg gcgtttatgt
     3421 taaacgtaac aagctcagcc attttgaatt cgatgccacc aactgtcctg acctgtttcc
     3481 cccactggtt gcacttgcct cacactgcga tggcgaatct aaaattttag gcgtcagccg
     3541 attaagggta aaagaaagcg acagggctgc aaccctggtg gaggaatttg ccaagctggg
     3601 tattgacatc aagattaagg acgatttgat ggttgttcag ggaggaaagg tgaaatcagc
     3661 atgggttcac tcgcacggcg accatcgcat tgcaatggcc tgtgcagttg ctgcactttc
     3721 gggtgaagga aatctgacca tcgagggagc cgagtcagta tcgaaaagct acccccgttt
     3781 tttcgacgat ctgaacagca taatgactaa tagctcgctt aatgtttaac taacctgtaa
     3841 tccagtgaat tcatttggta ggatattcag agtatcgcta tttggcgaat cgcacggaag
     3901 ggcaattggc gtagtagttg atggggtaaa gccagggctg cccctatcaa ccgatgattt
     3961 tgctgacgac atacgtcggc gtgggccggg actaccaggg acaaccccca gaaccgagaa
     4021 tgatttaccc gaaatggtgt caggttggtt caacggctat accacaggag caccgctggc
     4081 aattattttc agaaatacca acgtacggtc agacgactac gaacgcatta aggacttacc
     4141 tcgcccgggt catgccgatt ttgttgccta caaaaaattt agtgggtaca atgactatcg
     4201 tggaggtggt catttctctg gtagaatcac tcttgcgctg gttgcggcag gcgttattgc
     4261 aaaaaaaatg attgacccgg tggaggttca tgccaaattg gtcacagttg gcggaagcac
     4321 cgatatcgaa aataccataa acaaggccat gtcggctaac gattcggttg gggggcttgt
     4381 agagtgcagg gtttccggca tacctatcgg ttggggcgag ccattctttg attcggtgga
     4441 atcggtaatc agccatttgg cattcgccat tccgggggtt aagggtattg aatttggctc
     4501 agggtttgca gccgcctcca tgtttggttc tgcccataac gatgcgttta ttgatgcctc
     4561 gggagcaacc tcatccaaca atgctggagg cattttaggc ggcataagca acggaaacga
     4621 gctccagttt agggtggcag ttaagcccac atcgagcata gccagagagc aacaaaccat
     4681 taacatgagg acagggatgc cagagaaatt agtggttaca ggacgccatg acacctgcat
     4741 agcactaagg gttccggttg tgctggaagc tgtagcagcc attgcccttg ccgacctggc
     4801 cgccattcaa aacaagagta aaggataata taaacttgtc cggctcctga cggattcaag
     4861 attctagaac
//
LOCUS       DAOSHM010000113         4839 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_15635_length_4839_cov_4.127299, whole genome shotgun sequence.
ACCESSION   DAOSHM010000113 DAOSHM010000000
VERSION     DAOSHM010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 4839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4839
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15635_length_4839_cov_4.127299"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="PK839_10350"
     CDS             complement(<1..209)
                     /locus_tag="PK839_10350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG73176.1"
                     /translation="MVKDGLIAKILVSYWQKYYLESASKNVKKNGEPRKKKSFRACPI
                     KYFTYLGKKFNPMFKASYSTSNINS"
     gene            190..1089
                     /locus_tag="PK839_10355"
     CDS             190..1089
                     /locus_tag="PK839_10355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG73177.1"
                     /translation="MRPSFTIIVLLFSLFINAGEAADYLREFSRTGMADYKKEQEIVA
                     GYTSENLTNEISPFYNDSVALVRQKAYYLTYKNAIVRNEGRGNAVNILLKGCFDNDNG
                     VVYQNVSFLKEFNRADFDTQALDLLQAKLTSQRIAHYKEFVLLAGFLGTGKNILYQKF
                     IDPGTNEKQKWYLSLALARMGNPENISYCMNKVRNLPINDNVVDYVVPDLVYIRQKES
                     IDFCISILNGEEKLCHSLNPDVSESIHCGFRIMDILSPIIEGMPVLDESLNLSNADYE
                     KHLAKVRQWFASNPNYIIKYSTF"
     gene            1116..>4839
                     /locus_tag="PK839_10360"
     CDS             1116..>4839
                     /locus_tag="PK839_10360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG73178.1"
                     /translation="MRTFFRLSFTLTLFVCFSFRGISQTSGSSANEILSQVASQQQKN
                     EDKINAAFTAMEAVERAGAYIESLADLFGNGEVTLPVGIKKGDYELIIQKILYNKDTQ
                     RSQIYATCAFKFKEDGQPVVFEGISDIEGQKGLGTNGSLQLVAPVLRKLGNKVAIIFN
                     NGTKANFGCEGIESFTAKLSLVITSDKIVPVNASGKPTSEPIVVSFEGTFQNFDSYSV
                     SFNFDRSFCLKGLQDIIFTLNGAILDQDDIETPALVKFPQGYFTSEADNIKLWRGIAI
                     TSASVSLPPFLKKNQQSADSIQTPADSISNPADSLENQNTFKDRVTLRLKDVIIDENG
                     FSGFAEGQNIILSEQLNRSNWGISLTDFRVSLLKNEVKGLGFGGDLNIPPLGKNSLLP
                     YSASYNSSTEEYDFKVNFSGEFDFPVFHSTLTVDETSSIEVMIRDSEFYPTLNASGDI
                     TINAPINEADTSKKFSAPGIHFENLSISREKPNLTIGAIGVTGANQSPKFAGFDLTIS
                     NIQTFDSPATGSGLSFQTGVNLSQMFGGEAGLQLYGDYDNWKFKRVNLNKVGVNFKSG
                     AYSIYGGVEFKNGDTLYGTGFRGDVELSLLDNKFNLDAVAVFGKKDEFRYFLTDVYFE
                     TSPESGIFVPPVLCFYGFGGGLYRRMRQSLNPVLHTEFGNSLSGISYIPDNTKGMGFM
                     ASTKLGLKGSPSAFNASVAFEMQFNNSGGLNFVQLRGDAAFMDDPKKWGTLAGNINSH
                     VQKLEEAGGTISLSATSDLKVPENKETGFLTASMNINYDIANKVFSADLNTYLNAGFI
                     KGVGANDRMGWASAYFSPGSWHTYIGTPSNRLGIEILGLARSDGYFMIGDNIPELPPP
                     PPKVLQNFSPQMQNRLNTRNTENLASGNGIAFGSSLGVNFNAELRPFYASLGVGMGAE
                     FMLKNYGENAYCAGSSTTLGIGGWYARGQAWAWVEADIGMKATVFGKTRKFSILDIST
                     SALLAGAGPNPIYFTGAVGGHFRVMGGLISGHCNFDFEIGEECKIMAGSPFGEDIIAQ
                     LTPAAGETDVNVFAAPQALFNIPLELEMEIEEDDGAKAWYKVTLEEYSVFYKDTKQKI
                     EGKAQLNSQRNIIMLDPTEPFESKKAMVVYAKVGFKRKLNGQWIDVKGSDGKPVFEEK
                     SAEFTSGERPKEILPEHVKFSYPIANQYNFYPEEYPSGYVLITENYSYLFTTDKPQGY
                     DQVLLVEDSDGTRNETDFTFTTYTTSDLGEGIKMEID"
BASE COUNT         1361 a          988 c         1127 g         1363 t
ORIGIN      
        1 aatgaattga tatttgaagt agaatagcta gctttgaaca tgggattgaa ttttttacct
       61 aaataagtga aatattttat cgggcaagcc ctgaaactct tttttttacg gggctctccg
      121 ttttttttaa cattctttga tgcggattcg aggtaatact tttgccaata gctcactaaa
      181 atttttgcga tgagaccatc tttcaccatt atcgtccttt tattctcttt atttataaat
      241 gccggcgaag ctgctgacta cttgagggaa ttttccagaa cgggcatggc cgattacaaa
      301 aaggagcaag aaattgttgc cggctatacc tcagaaaacc tgactaacga aatatcccca
      361 ttctacaacg attctgtagc cctggttcgt caaaaggcat attatttaac ctataaaaat
      421 gctattgtca gaaatgaggg taggggtaat gcggttaata tccttctaaa gggttgtttt
      481 gacaacgaca atggcgtggt atatcaaaat gttagcttcc ttaaagagtt caaccgggca
      541 gattttgaca ctcaagcgct ggatttacta caggcaaaac tcaccagcca gcggattgcc
      601 cactataagg agtttgtatt gctggccggt ttcctgggta ctggtaaaaa tattctctac
      661 caaaaattca ttgatcccgg caccaatgag aagcaaaaat ggtacctgtc acttgcccta
      721 gcccgaatgg gtaatccgga gaatatatca tactgcatga ataaggttag gaacctgccc
      781 ataaatgata atgtggttga ttatgtggta cctgacttag tgtatattag acaaaaagaa
      841 agtatcgatt tttgcatttc tattcttaat ggcgaggaaa aactctgcca ttcattgaac
      901 cctgatgttt cggaatccat tcattgcggg tttaggatta tggatattct tagccctatt
      961 attgagggta tgccggtttt ggatgaaagc ttaaatttat ccaacgctga ctatgaaaaa
     1021 cacctggcta aagttaggca atggtttgcc agcaatccca attatatcat aaaatatagc
     1081 actttttaat aagagtcaac ttaatatatt ctctaatgcg aacatttttc cgtttatcgt
     1141 ttactttaac tctgtttgtt tgtttttcgt ttaggggcat ttcccagact agcggttctt
     1201 ctgctaacga aattctctca caggtggcta gccagcagca gaaaaatgaa gacaaaatca
     1261 atgccgcttt caccgccatg gaggcagtgg aacgtgcggg tgcatatatc gaatccctgg
     1321 ctgatttatt tgggaatgga gaggttaccc tacccgttgg tataaaaaag ggggactatg
     1381 agcttatcat tcaaaaaata ctctacaata aggataccca acgatcgcag atttacgcca
     1441 cctgtgcatt caaattcaag gaggatggac agcccgttgt ttttgaaggg atatctgaca
     1501 ttgaggggca aaaagggctt ggaaccaatg gttcgcttca gctggttgca cccgttctta
     1561 gaaagcttgg aaataaagtt gcaattatat tcaacaatgg aacaaaggca aattttgggt
     1621 gcgagggtat cgaatcgttt acagcaaaac tatcgctggt gatcacatcc gataaaatag
     1681 taccggttaa cgctagcggt aaaccaacct ccgaacccat tgttgtttcc ttcgagggta
     1741 cttttcagaa ttttgattcc tactcggttt cctttaactt cgatcgttcc ttctgcctta
     1801 aaggactaca ggatattatt tttaccctta acggggccat actcgaccag gatgatattg
     1861 aaactcctgc attagttaag tttcctcaag gctatttcac atctgaggcc gataatataa
     1921 aactatggag aggtattgca attacaagtg catcagtttc attgccccca tttttaaaga
     1981 aaaatcaaca aagcgcggat agcattcaaa ctccagcaga ttctatttca aaccctgccg
     2041 attctttgga aaatcagaat acatttaagg atagagttac acttcgattg aaggatgtaa
     2101 ttattgatga gaatggattt tcaggatttg ccgaaggcca aaatattatt ctatcggaac
     2161 agctgaacag aagcaattgg ggtatttccc ttacagattt tagggtaagc ctgcttaaaa
     2221 acgaggttaa aggtttgggg tttgggggcg atttaaatat tccgccattg gggaaaaact
     2281 cgttgctgcc ctattcggct tcctacaact cttcgaccga ggagtatgat tttaaggtga
     2341 atttttcggg agagttcgat tttcctgttt ttcacagcac attaacggtg gatgagacct
     2401 catccatcga ggtgatgata agggattctg aattttatcc cacattaaat gcatccggag
     2461 atattaccat aaatgcaccg attaatgagg ccgatacctc caaaaaattt tcggctcctg
     2521 gtatccattt tgagaattta tcaattagcc gggaaaaacc taacctgact atcggggcaa
     2581 ttggggtaac aggagcaaat cagtccccaa aatttgcagg tttcgacctg acaatctcca
     2641 acattcaaac atttgatagt ccagctacgg gtagcggtct ctcctttcag acgggtgtaa
     2701 atttgtccca aatgtttggc ggcgaggctg gcttgcagct ttacggcgat tatgacaatt
     2761 ggaagtttaa gagggttaac ctcaacaagg tgggcgtcaa ttttaaatca ggtgcctata
     2821 gtatttacgg cggtgttgag tttaaaaatg gcgatacgct gtacggcact gggtttaggg
     2881 gcgatgtgga gctctcctta cttgataaca agttcaacct cgatgcagtt gctgtcttcg
     2941 ggaaaaagga tgaattccgc tacttcttaa ccgatgtgta ttttgaaacc tccccggagt
     3001 cgggcatttt tgttccccct gtactttgct tttacggctt tgggggtggg ctataccgcc
     3061 gcatgcggca atccctgaac cccgtactcc atactgaatt tgggaattcc ctctcaggta
     3121 taagctatat acccgataat acgaagggaa tgggatttat ggcttctaca aagttaggat
     3181 taaaaggatc accatcggca tttaatgcca gtgttgcctt tgaaatgcag ttcaacaaca
     3241 gcggggggct caatttcgtc cagcttaggg gcgatgcggc atttatggat gaccccaaaa
     3301 aatggggaac gcttgccggg aatataaata gccatgtgca gaagctggaa gaggccggtg
     3361 gcaccatatc gctttcggct acaagcgatt taaaagttcc cgaaaataaa gagactggtt
     3421 ttctcaccgc atcaatgaat attaactacg atatcgcgaa taaagtcttt tcagctgatt
     3481 taaacacata cctaaatgcc ggatttataa agggggttgg agcgaatgac agaatgggct
     3541 gggcctcggc atacttttca cccggcagct ggcacaccta tatcggaacc ccatccaata
     3601 ggcttggaat agagatactc ggattggcac gatccgatgg ctactttatg atcggtgaca
     3661 atatacctga acttccaccc cctcctccaa aagttttgca gaatttcagc ccgcaaatgc
     3721 agaataggct gaatacccgg aataccgaaa atttggcatc ggggaatgga attgctttcg
     3781 ggtcttcgct cggtgtcaac tttaatgcag aattacgtcc gttttacgcc tcgttaggtg
     3841 tgggtatggg agctgagttc atgctgaaaa actatgggga gaatgcatac tgcgccggta
     3901 gcagcactac tctgggtatt ggtgggtggt atgcccgagg gcaggcttgg gcctgggttg
     3961 aggccgatat tggaatgaag gcaacagtat tcggtaaaac ccgaaagttc agcatcctgg
     4021 atatctccac aagcgcactg cttgccgggg cgggtccaaa ccccatctat ttcacggggg
     4081 cagttggcgg gcattttcgg gttatgggcg gactgatttc cggccactgc aatttcgatt
     4141 ttgaaattgg cgaggaatgt aaaatcatgg caggtagccc gttcggtgag gatataattg
     4201 cacagctcac tcccgccgcg ggcgaaacgg acgtgaatgt gtttgcagca ccccaggcgc
     4261 tgtttaacat tcccctggag ctcgagatgg agatcgagga ggacgacggg gccaaggcct
     4321 ggtacaaggt tacccttgag gagtactccg ttttttataa ggatacaaag caaaagattg
     4381 aaggtaaagc ccagttgaac tctcaaagga atattataat gcttgatcca actgagcctt
     4441 ttgaaagcaa aaaggcaatg gtggtttatg caaaggttgg ctttaagcgt aagttgaacg
     4501 gacagtggat tgatgttaag ggtagcgatg gtaagcctgt gtttgaggag aaatccgcag
     4561 agtttacctc tggtgaacga cctaaagaaa tccttccgga acacgtaaag ttcagctacc
     4621 caattgctaa ccagtataac ttctacccgg aggagtaccc atcgggatac gtattgatta
     4681 ccgagaatta ctcgtacctc tttaccaccg ataaaccaca gggctacgat caggtgctac
     4741 ttgtcgagga ttcggatggg actaggaacg agactgattt tacctttaca acctacacca
     4801 cttccgattt aggtgagggc attaaaatgg agattgatt
//
LOCUS       DAOSHM010000114         4780 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_15918_length_4780_cov_17.897143, whole genome shotgun
            sequence.
ACCESSION   DAOSHM010000114 DAOSHM010000000
VERSION     DAOSHM010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 4780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4780
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15918_length_4780_cov_17.897143"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..454)
                     /locus_tag="PK839_10365"
     CDS             complement(<1..454)
                     /locus_tag="PK839_10365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG73179.1"
                     /translation="MKIYKVFGLVAAMAVTLVSCETDVDDPAGVRGVGVYPEMSNIGP
                     AVFDSKNLATTYVQFTVGIDDPAVNQVVIVGSYGNVSRVEIQSYTAFPAVVKITLSDV
                     VSKFGITLADVKLNDEFTFEVVTVKNGKRYYSNAAIVAPVVCAYDPAFV"
     gene            complement(484..1935)
                     /locus_tag="PK839_10370"
     CDS             complement(484..1935)
                     /locus_tag="PK839_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HOG73180.1"
                     /translation="MRKTFNILVSGLLVAATWSCSEDTMDDINKNVNDPTNVSSNLTI
                     TDVMTSSAFSVTGSDLAFYASVYIEHNVGIWNQSYNAEIRSGEPTSATTYNNSWDAIY
                     GNLYNLKTIIKKCSAGGSEEGNYYTLGIAQVLTAYNLAILTDLMGDVPWTEALQPGFI
                     YTPKLDKQEDIYQVVFTMLDDAIANFDKTSLFPSLGAQDFLYAGNIGKWKKFAYGLKA
                     RYTMRLSYRTPDYANVITYANQSFASAAEQCQFNYNGTTSQSPFYRFFTDRDYFGASQ
                     SLHNKLVARSDPRDAKFFKKYPGADALVFAPNGTPDQVQKVYGISALSIITAPTYLLS
                     YHEIEFLKAEAYVRLGGAANLALAQTALQNAIRAAFQKVNIGLTQASADSYFTNEVLP
                     RFTANPLSEVMNQKYIAFFEEEAVEAYNDYRRLKAMGDNVIQLDNPKNATQFPLRFTY
                     GSSDVTTNVNVREAYGDGQYVYTENVWWAGGSR"
     gene            complement(1961..>4780)
                     /locus_tag="PK839_10375"
     CDS             complement(1961..>4780)
                     /locus_tag="PK839_10375"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOG73181.1"
                     /translation="ALGYAVQGVASEELAKTGNANLLSAMQGKVAGIDIKPSSGMPGA
                     SSQFVVRGARSFTGNNTPLYVVDGMPISSTAPYSTGNSVTGADISNRAIDINPADIES
                     VEILKGQAAAALYGIRASNGVVIITTKSGKGQQVGKPIITFSHNSNFDVVSRTPDYQT
                     TYAQGSYGVYLPTASYSWGPKIVDLPKDPTYGGDNNGHPGLYRVPQMETAGLDPWVAP
                     HVYNSWKDYFRTGYSTTNSINVMQATEQGNFALGFTYADQTGIALNTGMTRYNAKASA
                     DRNLGKNFKVGFNANYSKNNIDKLSGANDGSLTGVLAAPASYNLKGIPTHYPGNPYRQ
                     IYFRGGSFDNPYWVPDNNTFNEKTDRFFGNGFVEFNPKLADGMGLNVRYQAGIDTYTT
                     HYQDIFGFGSHNNGKGYMSNYGVTDGTFNSLLTANFNWKISDEMDFTAMVGNEVNNGK
                     SKYYSERGNDFNFGGWNHINNANIVTASESKYSDRTVGFFGNLSLSWKSMIFLNGTGR
                     YDVVSSMPRNNRSFFYPSVSLGFVASELDFIKNVNWISFLKLRGSYAEVGQAGSYVEN
                     YYVKPGYGGGFWSGNAPIIYPLGGINAYIPSSVQYDPNLKPQNTKSYEVGAELKFFNN
                     RLGIDYTYSKQNVTDQIFAVPLAGSTGASELVMNAGKVHTNSHEVVMYVTPIRTTNFQ
                     WDINLNYSKIENVVDELAPGVESIFLGGFTTPQVRAGIGYTYPVIYGSSFVRDSKGNI
                     VVEDNPGAWNHGMPKIGAPGVIGEVSPDFILGGTNTFTYKSLSLSVVLEWKNGGEMYS
                     GSNGLLDLYGRSAVTEDRESTFIFKGVKPDGTPNDIVRGGSNDPDAVQDLYANVLTNI
                     DEYYIHENSFLKLREVALRYQLPKLYKSVKVSASVYARNILLWTALRNLDPESSQGNT
                     NMGGSFERFSLPQTTTYGFGLDFTF"
BASE COUNT         1436 a         1043 c          929 g         1372 t
ORIGIN      
        1 ttacaaaagc aggatcgtaa gcgcacacta cgggtgcaac aattgcagca tttgaatagt
       61 accttttacc attctttacg gttacaactt caaaggtaaa ctcatcgtta agcttaacat
      121 ctgccaatgt aataccaaat ttggatacca catcacttaa tgtaatctta acaacagcag
      181 ggaatgcagt ataagattga atttctaccc tgcttacatt tccataggaa cctacaataa
      241 caacctggtt aacagcagga tcgtctatgc caactgtaaa ctgaacatag gtagttgcca
      301 gattctttga atcgaatact gcgggtccaa tattggacat ttcaggataa acgcctactc
      361 cccttacacc agctggatcg tcaacatctg tttcgcacga aactaatgtt acagccattg
      421 cggctaccaa accaaatact ttgtatattt tcatatccta ttcgttttaa ttatccaaac
      481 gagctaacga ctgccgccag cccaccaaac attttcggta taaacatact gaccatctcc
      541 ataagcttca cgaacgttaa catttgttgt aacatcgctt gatccgtagg taaagcgtaa
      601 tggaaattga gtggcatttt tcggattatc gagctgaata acattatcgc ccatagcttt
      661 caggcgacga taatcgttgt aagcctcaac agcctcttct tcgaagaatg caatgtactt
      721 ctgattcatc acctctgaaa gcgggtttgc ggtaaatctt ggtaacacct cgttggtaaa
      781 atatgaatcg gcacttgctt gggttaaacc aatattaacc ttttgaaagg cagctctaat
      841 tgcgttctga agtgctgttt gagctagtgc caggttggca gcacctccca atcttacata
      901 ggcttcagcc ttaagaaatt ctatctcatg gtaactcaag agatatgtag gagcagtaat
      961 tatagaaagt gcggatatcc cgtaaacttt ttgaacctga tcgggtgttc cgttaggtgc
     1021 gaatactagg gcgtcagctc ccgggtattt tttaaagaac tttgcgtccc gtggatcaga
     1081 acgggctacc agcttgttat gtaaactttg acttgctcca aagtaatccc tatcggtaaa
     1141 aaagcggtag aaaggacttt gagaggtagt gccattatag ttgaactggc actgctctgc
     1201 agcagaagcg aacgactgat tagcataggt aattacatta gcatagtcag gggttctgta
     1261 ggaaagacgc atggtataac gggcctttag cccataagca aactttttcc attttccaat
     1321 attccctgca taaagaaaat cctgtgctcc taaagaaggg aataaagatg ttttgtcaaa
     1381 atttgcaatt gcatcatcaa gcatggtaaa aacaacctga taaatatctt cctgcttgtc
     1441 cagcttagga gtatagataa agccaggttg cagcgcttca gtccagggaa catcccccat
     1501 caaatcggta aggattgcca ggttataggc tgtaagaacc tgagcaatac caagggtata
     1561 gtagttgccc tcctcagaac ctcctgcaga acacttctta ataatagtct tcaaattgta
     1621 aaggtttcca tagatagcgt cccatgaatt gttataggtg gttgcactgg taggctcacc
     1681 tgagcgaatt tcagcattgt agctttgatt ccaaataccc acattgtgct cgatatacac
     1741 cgaagcatag aatgccagat cgctaccggt aacagaaaac gctgagctgg tcataacgtc
     1801 ggttatggtt aaattagagc tcacgttggt aggatcgtta acattcttat ttatatcatc
     1861 cattgtatct tctgaacaag accaagttgc agcaaccagg agtccagaga ctaggatatt
     1921 gaatgttttt ctcataacgt ctatgtattt aaaatttaag ttagaaagta aaatctaagc
     1981 caaatccata ggtagtagtt tggggcaagg agaatctttc aaacgaaccg cccatgttag
     2041 tattaccctg agatgactct ggatcaagat tgcgtagcgc agtccataaa aggatgttcc
     2101 ttgcataaac cgaagcgcta actttaacac tcttgtaaag ttttggaagc tggtagcgaa
     2161 gagctacttc acggagttta aggaatgaat tttcatgaat gtagtactca tcaatgttgg
     2221 taaggacatt ggcataaagg tcctgaacag catcaggatc atttgaaccg ccccttacta
     2281 tatcgttagg agttccgtca ggttttaccc ctttaaatat aaaggtggac tcacgatctt
     2341 cggtaacagc agatcttccg tagaggtcaa gtagtccatt tgaccccgag tacatttctc
     2401 cgccattttt ccattcaaga acaacactta gggaaaggga tttgtaggta aatgtatttg
     2461 ttccaccaag tataaaatca ggagatactt caccaattac accaggggct ccaattttag
     2521 gcattccatg gttccatgca cctggattat cctcaaccac aatgttaccc tttgaatccc
     2581 ttacaaatga ggaaccataa attacaggat atgtatagcc tatacctgcc ctaacctggg
     2641 gagtggtgaa tcctcccaaa aatatacttt caacaccggg agctaactcg tcaaccacgt
     2701 tttcaatctt cgaatagttc aggttgatat cccattggaa gttagttgtc ctgattggtg
     2761 taacatacat tacaacctca tgagagttag tatgaacctt accggcattc ataaccaact
     2821 cagaagctcc ggttgaccca gcaagaggaa ctgcaaatat ctgatcggtt acattttgct
     2881 ttgagtaggt ataatcaata cctaacctgt tattgaagaa cttcaactca gcacctacct
     2941 cataggattt ggtattctgc ggttttaagt taggatcgta ctgaacactg ctgggtatat
     3001 atgcatttat acctcctaat gggtaaatga taggagcatt cccagaccag aacccaccac
     3061 catatccagg tttaacatag taattctcaa cataagaacc ggcttgtcca acctcagcgt
     3121 aggatcctct taactttaag aatgaaatcc agttaacatt tttgataaaa tccaactcag
     3181 aagctacaaa acctaaggaa acagatgggt aaaagaacga acggttattc cttggcatag
     3241 atgaaaccac atcgtacctt ccggtaccat tcaagaaaat cattgatttc catgatagtg
     3301 acaggttacc aaaaaatcct acagttctat ctgaatactt agattcgcta gcagttacga
     3361 tattagcatt attgatatgg ttccaaccgc caaagttgaa atcattacca cgctcactat
     3421 aatacttgct tttaccatta ttaacctcat ttcctaccat tgcagtgaaa tccatttcat
     3481 cggatatttt ccagttaaaa ttggcagtca gcaaggagtt gaatgttcca tcagttacac
     3541 catagttcga catgtacccc ttaccattat tatgacttcc aaaaccaaaa atatcctgat
     3601 aatgagttgt ataggtatca atacctgctt gatatcgaac attcaacccc atcccatcag
     3661 ccaacttcgg gttaaattca acaaaaccat tcccgaaaaa gcggtcggtt ttctcattaa
     3721 aggtattgtt atcaggtacc cagtaagggt tgtcaaaact tccaccacgg aaatagattt
     3781 gcctataggg atttccgggg tagtgagtgg gaataccctt taagttgtaa cttgccggag
     3841 cagctaaaac gccagttaaa gaaccgtcat ttgcaccaga aagtttatca atattgtttt
     3901 tagagtagtt agcgttaaac ccaaccttaa agtttttgcc caaattacga tcggcagaag
     3961 ctttggcatt atacctagtc ataccggtgt tcagggcaat ccctgtttga tcagcatagg
     4021 taaatcctaa ggcaaaatta ccttgttctg tagcttgcat tacgttaata ctgttggttg
     4081 tggagtaacc tgttctaaaa taatctttcc agctgttata tacatgagga gctacccatg
     4141 gatcgagtcc tgccgtttcc atttgaggaa ccctgtacag gccgggatga ccattgttat
     4201 cgcccccata ggtaggatcc tttggaagat ctacaatttt tggaccccag gaataagatg
     4261 cagttggaag atatacgccg taacttccct gcgcataggt agtttgatag tcaggtgtcc
     4321 tggaaacaac gtcaaaatta ctattgtgcg aaaaagtgat tataggcttc ccaacctgct
     4381 gtccctttcc acttttagta gtaataatta ccaccccatt agaagctctg attccataaa
     4441 gtgcagcagc agcttgccct ttcaggattt caacgctttc aatatcagca gggttgatat
     4501 caattgccct attcgaaata tcggcacctg taacactatt tccggttgaa tagggtgctg
     4561 tggacgagat aggcattcca tcaaccacat acaaaggggt gttgtttcct gtaaatgatc
     4621 gagcacctct aactacaaat tgtgaggaag cacccggcat accacttgag ggcttaatat
     4681 caatacctgc aactttaccc tgcattgcag aaagtaggtt ggcatttcct gtttttgcaa
     4741 gttcctcgct ggcaactcct tgaacagcat aacccaatgc
//
LOCUS       DAOSHM010000115         4408 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_17889_length_4408_cov_18.017919, whole genome shotgun
            sequence.
ACCESSION   DAOSHM010000115 DAOSHM010000000
VERSION     DAOSHM010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 4408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4408
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17889_length_4408_cov_18.017919"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2883
                     /locus_tag="PK839_10380"
     CDS             <1..2883
                     /locus_tag="PK839_10380"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOG73182.1"
                     /translation="CSDLEVNLTSTSTGGSLSYLWDLGNGQSATTTNVTHTFTNRGSI
                     DSVYTVQLLTINPLGCRDSIEQSITVHPKVEAEFVFAQQSQCTPFYIDLTNTSLNGNT
                     FNWHTHYNGDTLTINKNPFAYQFDNLTLNDILTDSIVLVSSDNVTGCTDTTFRLLQIY
                     PRVVSQFNVDRLAGCNPLTVNFSNSSSGLSTYLWEFGDGATSVDNNPAPHVYEHPYKD
                     QSKLFTVGLSAMNAFGCKDFKDTVITVYPLVKADFQWNRFEGCTPLTINLNNSSTSPL
                     YLYRWNFDDGSPFTFAEQPLTHTYTNATTTPPDILHPTITLRTSYVNDTTCIDSLKLP
                     VNVFPHIYPEFSYDSTGCHPLDVTFTNQTVAYGGVGNASYLWDLGTGVSSFDQNTTFG
                     YTNTSQTDDSTFTIRLRATSIHGCEDSITHQVVVHPRPYAAMELVGEYVSCPPFNVEI
                     DNNSIGTNLTYIYDFGDGNDSTTTSGANMHHIFHNLSSETRPYQIDLRAVTEYGCDNS
                     VSQTIYVFPEVVASYVANPGYQACSPFDVQFQNSSLNAKFYRWDFADGITSSYHEPAH
                     SFLNFDENDKQFDVVLYASSEYSCFDTDTQHIIVYATPIANIAVEPPLQVFPNATFNI
                     YNQSSPAANSWSYSWSFDDNQYSSDKNPGTHTYLKWGPKENGFKYNVIMHVASPHCQD
                     ADTSVVYLFPAEPVANFSGDIDSSCSPLTVHFINTSQWADSYLWDFGDSTTSAEREPI
                     HTYTQPGYYTARLTASGDGGQHFYYRIFRVYENPKADFAVYPQRVMLPDATIHAYNLS
                     TNFSRSFWDLGDGYQTWERDPMHTYTNLGEFKISLWTYMDYGDDVCIDSISKFPAVWV
                     EGVGYVNFPNAFKPNPAGPNGGLYDAIDYKNEVFHPYHFGVVEYKLMIFSRWGEQLFT
                     SNDVNIGWDGYVNGKLAEQGVYMWRAIGKFTNGKTFDKKGSVTLLR"
     gene            3012..4016
                     /locus_tag="PK839_10385"
     CDS             3012..4016
                     /locus_tag="PK839_10385"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HOG73183.1"
                     /translation="MLRGFIRHTCKGIAIAFLVLGSMGRSFSQDQQFTQFYSSPLFLA
                     PSFTGSIVGHRVVTNYRNQWTSIPGAYNTFSISWDYNSPAFRSGFGIIAMRDQAGAGN
                     LGNTKVGALYSYDIIPTPDWHIRPGLGFYVQQLSIDFGKLIFSDQLTSTPMPPTSVTQ
                     PGKQAVWDIDVSTSVVAYSDFIWFGAAWDHMLRPVATFYDEDSRVPYKYSFFGGYRYV
                     TKGFLLSKVEESITFSFNFRMQDIYRQLDLGLYWFSEPFTFGVWWRGMPKGQTRSRID
                     ALAFMLGYRWESISVGYSYDFTISKLGLGSGGSHEISLIYLFKMNPKRHWKAMPCPTF
                     "
     gene            complement(4070..4195)
                     /locus_tag="PK839_10390"
     CDS             complement(4070..4195)
                     /locus_tag="PK839_10390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG73184.1"
                     /translation="MDSPQGRIGKPLHNLRTSSFTSGLKIDGYVLPKGLIHVGSE"
BASE COUNT         1118 a         1165 c          995 g         1130 t
ORIGIN      
        1 tgctccgacc tggaggtaaa cctcaccagc acctcaacgg gtggcagcct cagctaccta
       61 tgggatttgg gcaacggcca aagcgccact accactaatg ttacgcatac cttcaccaac
      121 cgggggagca tagacagcgt ttacaccgtt cagctgctta ccatcaaccc gctgggctgc
      181 cgcgatagca ttgagcagag catcaccgtc cacccaaagg tggaggcgga gtttgtgttt
      241 gcccagcagt cgcagtgcac cccattctac atcgacctca ccaacacctc gctcaacggg
      301 aacaccttca actggcatac ccattacaat ggcgacacgc tcaccatcaa caagaatccg
      361 tttgcctacc agttcgacaa cctcaccctg aacgacatac ttaccgacag catcgtgctg
      421 gtaagctccg acaacgtaac cggctgcacc gataccacct tccggttgct gcagatttac
      481 ccgcgggtgg tatcgcaatt caacgttgac cggctggccg ggtgtaatcc cttaacggtt
      541 aacttttcca atagttcttc ggggctgtca acctacctgt gggagtttgg cgacggggct
      601 acatcggtgg acaataaccc agccccacat gtgtacgagc atccatacaa ggatcagtca
      661 aaacttttta ccgttgggct ttctgcaatg aatgcctttg gatgcaagga ttttaaggat
      721 accgtcataa cagtttaccc gctggtaaag gccgattttc aatggaatag gttcgagggc
      781 tgcacaccgc tgaccatcaa cctcaacaac tcgtcaacct cacccttata cctttaccgt
      841 tggaatttcg acgatggatc gcctttcacc tttgcagagc aaccgcttac gcatacatat
      901 accaacgcaa ctaccacgcc gcccgacatc ctgcacccaa ccatcacgct gcggactagc
      961 tacgtgaacg atactacctg tattgactcg ctgaagctgc cggttaacgt gttccctcat
     1021 atctatccgg aattttcata cgattccacc gggtgccatc cgctggatgt tacctttacc
     1081 aaccaaacag ttgcttatgg aggggtgggc aatgcgtcct acctgtggga tttgggaaca
     1141 ggggtaagct catttgacca gaatactaca ttcggctata ccaacacaag tcaaactgat
     1201 gacagcactt tcaccattcg gctaagggca accagcatac atgggtgtga ggactcgatt
     1261 acccatcagg tggtagttca cccacgtccg tatgccgcca tggagctggt tggtgaatac
     1321 gtatcctgtc cgccctttaa cgtggagatt gataacaact ccattggcac caaccttacc
     1381 tatatctacg actttggaga tggcaatgac tccacaacaa caagcggggc caacatgcac
     1441 catatattcc ataacctctc aagtgaaacc cgaccttacc agattgacct cagggctgtt
     1501 accgagtatg gttgtgacaa ttctgtttcc caaaccatct acgttttccc cgaggtggtt
     1561 gccagctacg ttgccaatcc gggttaccag gcctgcagcc cgtttgatgt gcagtttcaa
     1621 aatagctcgc tcaacgctaa attttaccgt tgggactttg ccgatggcat tacctcttcg
     1681 taccatgaac ctgcccattc atttctgaat tttgatgaaa acgataagca attcgacgtg
     1741 gtgctctatg cttcttcaga atatagctgc ttcgataccg atacccagca tatcatagta
     1801 tatgcaacgc caattgccaa cattgccgta gaaccgcccc tacaggtttt ccccaacgcc
     1861 accttcaaca tttacaacca gagcagcccg gcagccaaca gctggagcta cagctggagt
     1921 tttgacgata accagtactc ctccgataaa aacccaggaa cccacaccta ccttaaatgg
     1981 ggaccaaagg aaaacgggtt caagtataat gtaatcatgc atgtggcgag cccacattgt
     2041 caggatgcgg ataccagcgt cgtttacctg ttccctgccg agccagttgc caacttctcg
     2101 ggtgatattg actcgtcctg ctcaccgctc accgttcatt ttataaatac ttcgcagtgg
     2161 gcagacagct acctatggga ttttggcgat agcacaacat ctgccgagcg cgagcccatc
     2221 cacacctaca cccaacccgg ttactacact gccaggctga ctgccagcgg cgatggcgga
     2281 cagcattttt actaccgaat tttcagggtt tacgaaaatc caaaagctga ttttgccgtt
     2341 tacccccaac gggtaatgct tcccgatgcc accattcatg cctataacct gtccaccaac
     2401 tttagccgca gcttctggga tttgggcgat ggctaccaaa cctgggagcg cgatccgatg
     2461 catacgtaca ccaacctggg agaatttaaa atttcgctat ggacatacat ggattatgga
     2521 gatgatgttt gcatagactc catttccaag ttccccgctg tttgggtgga aggggtaggc
     2581 tatgtaaact tccccaacgc ctttaaaccc aaccctgcag ggcctaatgg tggcctctac
     2641 gatgccattg actataaaaa cgaggttttc cacccctacc attttggagt ggtggagtat
     2701 aagctgatga ttttctcccg atggggcgag cagcttttca cctcaaacga tgtgaatata
     2761 gggtgggatg gatatgttaa tgggaagctg gccgagcagg gagtttacat gtggagggct
     2821 ataggtaagt ttacaaatgg caaaaccttt gataaaaagg gtagcgttac cctgctgagg
     2881 tagcagggtt cgaaaaaaac ctgcattatc agcccatatt ttatagagaa atagaatgaa
     2941 aagtagcttc aacttaagat agtttttaca aaggcttgga cgcatttaaa tttttgataa
     3001 gtcgttgctt gatgttaaga ggatttataa gacatacatg taagggtatt gctatagcat
     3061 ttttagtgct gggcagcatg ggtagatcct tctcccagga ccagcagttc acacaatttt
     3121 actccagccc attattcctt gccccctcct ttacgggcag cattgttggt catagagtag
     3181 taactaatta tagaaaccaa tggacatcta tacccggtgc gtacaatacc tttagcatct
     3241 cttgggacta caattctcct gctttccgaa gtggttttgg tattatagcc atgcgcgacc
     3301 aggccggagc gggaaacctg gggaacacca aggttggggc gctttactcc tacgacatca
     3361 ttccaacgcc cgactggcat atacgacctg gtttaggatt ttacgttcag cagctatcca
     3421 ttgatttcgg gaagctaatt ttcagcgatc agctgacctc cacacccatg ccacccacat
     3481 cggttacgca gccaggtaaa caggcagttt gggatattga tgtatccacc tcggtggtag
     3541 cctactccga ctttatctgg tttggtgcag cgtgggacca catgcttcgg ccagtggcga
     3601 cattttacga cgaggattca cgcgttccct ataaatattc cttttttggt ggataccgat
     3661 atgttaccaa ggggtttctg cttagcaagg tggaggagtc catcaccttc tcgttcaact
     3721 tccgaatgca ggatatatac cgccaactcg acctgggtct atactggttt tccgaaccgt
     3781 ttacatttgg tgtttggtgg cgcggcatgc caaagggcca aaccagaagc aggatcgatg
     3841 cattggcatt catgttggga tacaggtggg aatccatcag tgtaggctat agctacgatt
     3901 ttaccatttc caagctgggt ctaggctcgg gtggctcgca cgagatttcg cttatctacc
     3961 ttttcaagat gaatccgaaa aggcactgga aggccatgcc ttgcccaaca ttctaaaggg
     4021 agggaaatac aggtaaaagt taatgatgat aattttgact gtcacccgtt tattcagacc
     4081 caacatggat aagccccttg ggcagaacgt atccatcaat tttaagccct gaagtgaagg
     4141 agctggttcg caaattgtga aggggtttcc ctatcctgcc ctggggagag tccatcacag
     4201 actgccaatc ttttaaatct ttagccttgc tcttatgtat ttttattacc gttgcgatgc
     4261 aaaggtctaa aattatgctt ttgagcggcc gctacttaca tcgccgaaac ttccgtggtc
     4321 accaaaaaac ccaaggcttt gccctgggct aggctgtttc gcccctaggg gctcttgttt
     4381 ccacacactc caaactcttc gtttcaaa
//
LOCUS       DAOSHM010000116         4244 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_18836_length_4244_cov_8.064455, whole genome shotgun sequence.
ACCESSION   DAOSHM010000116 DAOSHM010000000
VERSION     DAOSHM010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 4244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4244
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18836_length_4244_cov_8.064455"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1351)
                     /locus_tag="PK839_10395"
     CDS             complement(<1..1351)
                     /locus_tag="PK839_10395"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG73185.1"
                     /translation="MEKNCLKKVMLVTILAIASSLPLSAQDDNQVNVDSMLNSAKSKL
                     YSSPNEAFELAQQVVELSVEQGNQLLLANGYKLLGAYYSDIKNDFDLAKLNYQKADSI
                     FRHNKGEEYAKGIGAIYHSYGVIDHRLGNYWEAIQYYYNALRVLDSINDNEIRPKTLN
                     NLSNLYAFLKDYSRAEKYSRECLRVSIENGNKQMISVVSVSLADALISQGKFEEVLSL
                     LLKSKEIATEQENLYILELVHLNLGSYYYLSENNYEKSIGEYLQALNYAKALGSDWEV
                     MRITTNVSEVYFLDNQLGKAMEYAEKSLGIAERMGTGDIKQRALSILAKVYASKGQYS
                     SAYENLLKSYIIRDSVFNDSKQMHVNYLESRYHSEMKEKEIIILKNTQREHELILKRR
                     KYQLIILITIILIVALVAAFIIVRNKYRQLLDAQKLHIQEQQIKQLEQEKQLVATQAI
                     "
     gene            1707..2282
                     /locus_tag="PK839_10400"
     CDS             1707..2282
                     /locus_tag="PK839_10400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG73186.1"
                     /translation="MKQLTTMKRITFLLVAMCFTTAMASAQNYFTKGAKVINATVGFG
                     NSLYTGSGYTGKVPPIAASFEVGILDNVFDEKSSIGVGGYLGYTSAKWESGGYGWRYS
                     DFIIGVRGSFHYQLIDKMDTYTGLLLGYDIVRATEIGDHTLWSGSSALSSGLAWSWYV
                     GGRYYFNDKFAGLVELGYGIAYFNIGLSVMF"
     gene            2335..4014
                     /locus_tag="PK839_10405"
     CDS             2335..4014
                     /locus_tag="PK839_10405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOG73187.1"
                     /translation="MKKLYLLLALLALLFVMPSCETEDETEVTVITGTISTPITWKAG
                     ETWVVEGSVYLEADLIIEPGTTVEFKKDAVVYVGYSAPASITALGTADKPIVFRGYEG
                     QTWKGIELYAHNSATKSAFTYCTFKQLGGDYALLIYGTKAKLDHCSFTECAKSAIHVL
                     GGGDFNGFTNNTLQDCGAFLMEIDADVAGSLGTTNTFTATGNKGVRISGSRNVTRAAT
                     WSSIGAPYVIANEQYIQVDADLTIAAGTTVLFEPHSMLEVGYSEYASLKAVGTAQAPI
                     TFTSASANPQPGDWYGIQFYSKNSSTTSELTFCTIEYAGTQSYPALFIGDTQVKLNDC
                     TVAKGQYYGVEVDGNAKFTQCMRNTIKEFGNHPMRVDVNAVYTIDASNTILSDGNFGV
                     YIPNYDLTGTVTWNKHEVPYFVEFIRFNNNAVLTIAPGTTVKFMAEGYFEVGYDAYGK
                     LIAEGTANEPITFTSAASSPSAGDWGGLQFYSQTSAGTKLNNCLVAYAGYPGYDTGAI
                     YINECGSAISLANTQIAHSNACDVYVYYSATPAMVNVTNHNGEQANVCVVN"
BASE COUNT         1213 a         1045 c          862 g         1124 t
ORIGIN      
        1 gaatggcctg cgtggcaacc agctgcttct cctgctctag ctgtttaatt tgttgttctt
       61 gaatatgaag tttttgtgca tctagcaact gtcgatactt atttctaaca ataataaacg
      121 cagcaactaa ggcgactatt aatattattg taatgaggat tattaattga tattttcttc
      181 ttttcaatat aagttcatgt tctcgttggg tattcttaag gattattatt tccttttctt
      241 tcatctcaga atggtaacgg gattctaaat aatttacgtg catttgttta ctgtcattaa
      301 atacactatc cctaataata taggacttta gtagattttc gtacgcacta gaatattgcc
      361 ctttagaagc ataaaccttc gccaaaatcg ataaagccct ttgctttata tctcctgtcc
      421 ccattctttc ggctattcca agagattttt cagcatattc cattgctttt ccaagttgat
      481 tgtctagaaa atagacttcc gaaacattgg tggttatgcg catcacttcc caatcactac
      541 ctaatgcctt tgcataattt agagcctgaa ggtactctcc tatagatttt tcatagttat
      601 tctcacttaa ataataataa ctacccaaat ttaaatgaac caactccaat atgtaaagat
      661 tttcctgttc ggtagcaatt tcctttgatt tgagcaacaa actaagaact tcttcaaatt
      721 ttccctgaga tatcaatgca tcagccaaac tcacgctaac aacagatatc atctgcttat
      781 taccgttttc tatactaact cttaaacact cccttgaata tttttctgcc ctactataat
      841 cctttaaaaa agcataaagg tttgacaggt tatttagggt tttaggtcga atttcattat
      901 cgttaatgct gtccaaaacc ctaagcgcat tataataata ctgaattgcc tcccagtaat
      961 tacctaagcg atgatcaata acaccataac tatgatagat tgcccctata ccctttgcgt
     1021 attcctcccc cttattgtgc ctgaaaatac tatcagcctt ttgataattt aactttgcta
     1081 aatcgaaatc atttttaata tcggaataat aggcacctaa taacttatat ccattagcca
     1141 ataacaattg atttccctgc tcaacagaca actcaactac ttgctgcgct agttcaaaag
     1201 cctcattggg agaagagtaa agttttgatt ttgccgagtt caacattgaa tcgacattaa
     1261 cctgattgtc atcttgagca cttaaaggta aagaactcgc tatagccaaa atggtaacaa
     1321 gcataacctt ttttaaacaa ttcttttcca tactcttatg ctactttatt attggtcttt
     1381 ttgaggcgaa ataaaaatta gccacgtcat aaaaaatcat catacgccag acaactctaa
     1441 tttaggtaaa agaaatatac cattaagaaa aacaggagca attctaaaat aagaataaag
     1501 tatttggctt atgcaaaaag ccttcataag aatcacacat atatctctcc atacctataa
     1561 ataacgttga ctatcccaat gcaaatttac atcttacatc gtcaaaaaaa tacccccaaa
     1621 agggggtagt gtaaaaaaac atagccacct acttttgcat gagaaaagaa actcacacaa
     1681 gagttctgaa acaaaaatta actactatga aacaattaac tactatgaaa cgcattacct
     1741 ttttgctagt tgcaatgtgc tttaccacag caatggcaag cgcgcagaat tactttacaa
     1801 agggagcaaa ggttattaat gccaccgttg gctttggtaa ctcgctctac acaggaagcg
     1861 gctacacagg caaagttcca cctattgcag catcgtttga agtgggtatt ctcgacaatg
     1921 tgttcgatga gaaaagctcc attggtgttg gcggatacct aggttacact tccgctaagt
     1981 gggaatcggg tggatacggc tggcgctact ccgactttat tattggtgta agaggctcat
     2041 ttcactacca gcttattgat aaaatggata catacacagg cctactactt ggctatgaca
     2101 ttgttagggc taccgaaatt ggcgaccaca cgctttggag tggcagttcg gccttgtcga
     2161 gcggtttagc gtggtcctgg tacgtgggtg gacgctacta ctttaacgac aaatttgctg
     2221 gccttgtaga gctaggttac ggtattgcct acttcaatat aggattatcg gtaatgttct
     2281 aagaatcatt cttttaaaac aaaaacgtta attccaactg ctaaaatcaa aaaaatgaaa
     2341 aagttgtact tactactagc actactggca ctactatttg taatgccatc gtgtgagaca
     2401 gaagacgaaa ccgaggtaac cgttataacc ggaaccatat cgacccccat tacatggaaa
     2461 gcaggggaga catgggtggt tgagggcagc gtttacctgg aggccgacct gattattgag
     2521 cccggtacca ccgtggagtt caagaaggac gccgtagtgt acgtgggcta ctccgcaccg
     2581 gccagcatca ccgcgctggg cactgccgat aagcccattg tgttcagggg ctacgagggc
     2641 caaacttgga agggcattga gctctacgcc cacaacagcg ccacaaaatc cgcctttacc
     2701 tactgcacct ttaagcagct gggcggggac tacgccctgc tcatctacgg gactaaggcc
     2761 aagctggacc actgctcctt taccgagtgc gccaaatcgg ccatccacgt gctgggcggt
     2821 ggcgacttca acgggttcac caacaacacc ctgcaggatt gcggagcctt ccttatggag
     2881 attgacgccg atgtggcggg ctctctgggc accaccaaca cctttaccgc tacggggaac
     2941 aagggcgtgc gcattagcgg gagccgcaac gtaacccgtg ccgctacgtg gagcagcatt
     3001 ggcgcgccct acgttattgc caacgagcag tacattcagg tggacgccga cctgaccatt
     3061 gccgctggaa ccaccgtgct ctttgagccc cacagcatgc tggaggtggg ctacagcgag
     3121 tacgcttcgc ttaaggccgt gggaacagcc caggcgccca tcacctttac ctcagcatcg
     3181 gccaacccac agccgggcga ctggtacggc atccagttct actccaagaa ctcgtccacc
     3241 acatcggagc tcaccttctg caccattgag tacgcgggaa cccagtcata ccctgccctg
     3301 ttcatcggcg acacccaggt taagctcaac gactgcaccg ttgccaaggg ccagtactac
     3361 ggcgtggagg tggacggcaa cgccaagttc acccagtgca tgcgcaacac cattaaggag
     3421 tttggcaacc accccatgcg cgtggacgtg aacgcggtgt acaccataga cgcatccaac
     3481 accattctct ccgatggtaa ctttggcgtg tacatcccca actacgacct taccggaacc
     3541 gtgacctgga acaagcatga ggtgccctac tttgtggagt tcatccgctt taacaacaac
     3601 gcggtgctaa ccattgcccc gggaaccacc gttaagttca tggccgaggg ctactttgag
     3661 gtgggctacg atgcctacgg caagctcatt gccgagggta ccgccaacga gcccataacc
     3721 tttacttcgg cggcgtccag cccttcggca ggcgactggg gcggactcca gttctacagc
     3781 cagaccagcg cgggaaccaa gctcaacaac tgcctggtgg cctacgctgg ctaccccggc
     3841 tacgacaccg gggccattta cataaacgag tgcggtagcg ccatatcact agccaacacc
     3901 cagattgcac acagcaatgc atgcgacgtg tacgtgtact actccgctac acctgctatg
     3961 gttaacgtga ccaaccacaa cggggagcag gctaatgtgt gcgtggtgaa ctaagaacga
     4021 acaaaaaaat gaaagagaag agggtgtcca aaaaataaag gacaccctct tttcgttccg
     4081 acacgtacct gagttaacct tcaaagactc aatccacaaa aaaataaact gcctttctag
     4141 atgcctctta ttgttagcct caagtgaata attacaatgc aacttatccc ctcgtaaggt
     4201 taactctctt aagatagaat acttgtcaag tttcacaagg ttgt
//
LOCUS       DAOSHM010000117         4203 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_19077_length_4203_cov_23.817985, whole genome shotgun
            sequence.
ACCESSION   DAOSHM010000117 DAOSHM010000000
VERSION     DAOSHM010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 4203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4203
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19077_length_4203_cov_23.817985"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1176
                     /locus_tag="PK839_10410"
     CDS             <1..1176
                     /locus_tag="PK839_10410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG73188.1"
                     /translation="SWGIMRFATDNETYIVPIFFSLLGSFFFLRGTERSGKGSIFWSG
                     FFSAFAILFHQVMIFWWIAIATGVIVRNRKREIFQFFCPALTVPAAYLIAVWLEYGSI
                     RVPVIVKFGLSDYYNGAAKLEFNGRVLLMWLLSLFRSFFQAHGYMFNLFRQSWTWVIA
                     GISAVMLTGYGLTNAIKNGKIQSSAFKRPVWIFALAALLHIFFALLSGGNAEFMAMLP
                     ALLAIVLSVIIDINPKALVITALGMLAWNITIGLIPLKYYTLDGSSMVVNSILANPQP
                     SKSAYLLHSEQRIKNELEYRQISQKPVLLGRARYNTQQLTDSLEVLLEEGFAVYTDDY
                     LRPQTVSREYIVEDHPASDDLWAPFRLIPCDSVVTLTGTYSLYRVLPPVASSLHPER"
     gene            complement(1151..2014)
                     /locus_tag="PK839_10415"
     CDS             complement(1151..2014)
                     /locus_tag="PK839_10415"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOG73189.1"
                     /translation="MKHIVVPIDFSKESLNGLRLAIIFANRFGSDIQMVHVQKFPSEY
                     TPGQQEEDRRNKEKKFMELVEEYSPKMLESANLTYILKKGKVYSEVVNQAQAFEDSAI
                     ICSTHGASGFEEFFIGSNAFRIISATDRPVITIRHGSMPRDIQKIVLPIDITTDTRQK
                     VPVTADIAKAFDAEIHVVAVASSQSDEIDLKLNAYSRQVTTYLKNREVRFQTSSLRGD
                     NITNITIEYALDVNADLISIMTEQNFSFSDFVLGSQAQQMLSKSPIPVLSVTPKEIFI
                     MGSFNVQGGGY"
     gene            complement(2273..3379)
                     /locus_tag="PK839_10420"
     CDS             complement(2273..3379)
                     /locus_tag="PK839_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04133 family radical SAM/SPASM protein"
                     /protein_id="HOG73190.1"
                     /translation="MTATAIPFRKKLALNLFKTYRRNAAKLHELNYVLWEATLRCNLS
                     CLHCGSDCKMDASVGDMPADDFLRVMDQVAEVANPNSTMVVFTGGEPLLRNDLEQVGL
                     SLYRKGFPWGIVTNGMLLSKQRIDSLVDSGLRSVTVSLDGLEASHNWLRGSGMSFSRA
                     LEAVRLLCAVPDVRFDVVTCVNRRNFGELNKLLELLLQVGVKEWRIFTIFPIGRASRI
                     PELQLQPEEFKQLFDFISWTRKENKIKLNYGCEGFLGSYEGEVRDSLFFCRAGINIAS
                     ILANGSISACPNLRDNFIQGNIYKDNFRNVWEQRFLTFRDRSWTRTGACADCDFYGYC
                     EGNGMHLRNEQNGELLFCHLQRILEGERILSGKA"
     gene            complement(3376..3816)
                     /locus_tag="PK839_10425"
     CDS             complement(3376..3816)
                     /locus_tag="PK839_10425"
                     /inference="COORDINATES: protein motif:HMM:TIGR04134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM-associated putative lipoprotein"
                     /protein_id="HOG73191.1"
                     /translation="MKKAKRIILVSYNVLITCLMAILGFLSSCRDTPADEYGSPHALF
                     KAKGNVSSAAAQQPIEGIKVTSQIDTVFTGIEGQFEINAGVDLPTGQSFNLKFQDSDG
                     AANGEFQELDTIVEFNNPVFTGGDGHWYQGETEKEFNVQLKGKE"
BASE COUNT         1095 a         1036 c          930 g         1142 t
ORIGIN      
        1 agttggggaa ttatgcgttt tgcaaccgat aatgagacct atatagttcc catatttttc
       61 tcgctgttgg gaagtttttt ctttctaagg ggaaccgaac gttcagggaa aggcagtatt
      121 ttctggtctg gttttttttc agcatttgcc attctgttcc accaggtaat gattttttgg
      181 tggattgcaa tagcaacggg agttatagtt cgaaatagaa aaagggaaat attccaattt
      241 ttctgccctg ctctaactgt gccagccgcc tacttaattg ccgtttggct ggagtatggc
      301 agcatcaggg ttcctgtgat agtgaaattt gggctatcag actactacaa tggagctgca
      361 aaacttgagt ttaacggcag ggtgctcttg atgtggttgt tgagcttgtt tcgctcgttt
      421 ttccaggctc atgggtacat gtttaacctg tttaggcaaa gttggacatg ggtgatagca
      481 ggaatttcgg ccgttatgct aaccggatac ggcctaacca acgctattaa gaatgggaaa
      541 atccaaagtt cagcatttaa acggccagtt tggatttttg cattggctgc tctactgcac
      601 attttctttg ctcttttatc cggtggtaat gccgaattca tggccatgct tcctgctcta
      661 ctagccattg tgctttcggt aataatcgat atcaacccaa aagccttggt aattacggct
      721 ctgggaatgt tagcatggaa tattaccatc ggactcattc ctctaaaata ctacacgctt
      781 gatggcagca gtatggtagt taacagcatc cttgcaaatc ctcaaccatc aaagtcggct
      841 tacctgctac atagcgagca gcggatcaaa aatgagctgg agtacaggca aatttcgcaa
      901 aaaccagttt tattgggcag agccaggtat aacacccaac agctaaccga tagtctggag
      961 gtattgctgg aggaaggttt tgctgtttac accgacgact acctgcgacc gcaaacagta
     1021 tcgcgtgagt acattgttga agatcatccg gcatctgatg atttgtgggc tccctttcgc
     1081 ctaataccct gcgattcagt tgttacgcta actgggacct actcgcttta ccgtgtttta
     1141 cctcctgttg ctagtagcct ccaccctgaa cgttgaagct acccatgatg aaaatttcct
     1201 tgggtgtaac gcttagcaca ggaatagggc ttttgctgag catctgctgg gcctgactac
     1261 ccagcacaaa atcgctgaag ctgaagttct gctcggtcat tatgctgata aggtcggcat
     1321 taacatcaag agcatactca atggtaatat tggtgatatt atcgcctcgt aggctggatg
     1381 tttgaaagcg gacctccctg tttttcaggt aggttgtaac ctgccgggag taggcgttga
     1441 gctttaggtc aatttcgtcc gactgggagg aggcaacggc aacaacatga atttcggcgt
     1501 caaaagcctt ggcaatatcg gccgttaccg gaactttctg ccgggtatcg gtggttatat
     1561 caattggaag cacgattttt tggatgtccc tgggcatgga gccatgcctg atggttataa
     1621 ccggcctgtc cgtagccgaa ataatacgga aagcgttgct cccaatgaag aactcttcaa
     1681 accccgaagc gccatgggtt gagcatatta tagccgaatc ctcaaaggcc tgtgcctgat
     1741 tgaccacctc gctgtaaact tttccctttt ttaggatgta agtgaggttg gcactttcga
     1801 gcattttggg tgagtactct tcaaccagct ccataaactt tttctcctta ttccgacggt
     1861 cctcctcctg ctgtccgggg gtgtattcgg aagggaactt ctgtacgtgc accatttgaa
     1921 tatcgctgcc aaaccggttg gcaaatatga tggccaaacg aagaccgtta agagattcct
     1981 tcgaaaaatc gatggggact acaatgtgct tcatggctta agggttcagg ttaaacgtaa
     2041 gcaaatctac gtcaaattat tccgttaacc aatgcgacag gtcaaattta ttttacatca
     2101 gggttggaat aaccataggc tcaggattac gcaaagttcc gtaaatcgcc cacaacctca
     2161 ttgctgccgc ctgctgcaaa tcgtttcgaa tagtagctgc aaaaaacctg cttgcctgaa
     2221 cccttcaccc taattaatgg aatagagatc attcacacta gaccccgcca agctaggcct
     2281 ttcctgaaag aatcctttct ccctccagta ttctctgcag atggcagaaa agtagctctc
     2341 cgttttgctc atttcgtagg tgcatgccgt tgccctcgca gtagccataa aaatcacaat
     2401 cggcacaagc acctgttcgg gtccagctcc tatcccggaa agttaaaaat cgttgctccc
     2461 acacattcct aaagttgtcc ttgtaaatat tcccctggat gaagttgtcc ctcaggttgg
     2521 gacaggctga aatagagcca ttggccagta tggaggcaat gttgatgcca gcgcggcaaa
     2581 aaaagagact atcgcgaact tcgccctcgt agctgcccag aaagccttca catccgtagt
     2641 tgagcttaat tttattctcc tttcgagtcc agctgatgaa atcgaaaagc tgcttgaact
     2701 cttcaggctg cagctgcagc tcggggatgc ggcttgccct tccaatcgga aaaatggtga
     2761 atatccgcca ctccttaact cccacttgaa gcagcagctc cagtagcttg ttcagctcac
     2821 caaagttccg ccggttaaca cacgttacca catcaaacct aacatcgggg acagcgcaga
     2881 gtaacctaac ggcttcaagc gcccgggaaa aactcatccc cgagcccctc agccaattgt
     2941 gcgaggcctc caacccgtca aggctaaccg tcacagacct aagccctgaa tcaactaagg
     3001 agtcgatccg ctgcttgctc agcaacatcc cgttggttac aattccccag ggaaaacctt
     3061 tccggtaaag gcttaacccc acctgttcca aatcatttcg taaaagaggc tcaccaccgg
     3121 taaacacaac catggtgctg ttgggattgg caacttcggc aacctggtcc attacacgca
     3181 agaaatcatc agcaggcata tcgcctacag aggcatccat tttacaatcg cttccgcagt
     3241 gaaggcaact caggttgcag cgtagggttg cctcccaaag aacgtagttt aactcgtgca
     3301 gctttgccgc attacgccgg taggtcttga aaaggttaag ggctagcttt ttcctaaaag
     3361 ggatagcggt agctgtcatt ctttcccctt cagctgaacg ttaaactcct tttcagtctc
     3421 accctggtac caatggccat cgccaccagt aaaaaccgga ttattgaact caacaatggt
     3481 atccagctcc tgaaactctc cgttcgccgc tccgtcgcta tcctggaact taaggttaaa
     3541 cgattgcccg gtaggtaaat ccactccggc gttaatttcg aactgccctt cgattccggt
     3601 gaacaccgta tctatctgcg acgtcacctt aataccctca atgggctgtt gggctgcagc
     3661 agacgaaaca tttccctttg ccttaaacag ggcatgtgga gaaccatact catcggccgg
     3721 ggtatccctg catgaggata aaaatcccaa aattgccatg aggcaggtaa tcagcacgtt
     3781 gtaggaaaca agaattatac gcttagcttt cttcatagct gacatattaa actacaattt
     3841 aagcaagtta aaaaaaaagg acaaaccagt aagtgccggg taaaatattg ataggaaata
     3901 aaaaaattgt caccaaaagt gctgtggaga agcaagatac cacacgaaat aatttatacc
     3961 tgagtgaata aaaatccctt agctacctta cggttgtcct cggcgttttc cgccggctgg
     4021 tggatcggtt cgtgacgtgt ggcaatccta tccctactct caacaacata ctacgcccat
     4081 ccttatgttt gtagctgata atcccgctga attatcttgt tactacccat tactttctcc
     4141 ctactaccac ttactttttt cttcttctac actaccacac gagaggacta acttcgttga
     4201 gct
//
LOCUS       DAOSHM010000118         4187 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_19190_length_4187_cov_5.972652, whole genome shotgun sequence.
ACCESSION   DAOSHM010000118 DAOSHM010000000
VERSION     DAOSHM010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 4187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4187
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19190_length_4187_cov_5.972652"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3400
                     /locus_tag="PK839_10430"
     CDS             <1..3400
                     /locus_tag="PK839_10430"
                     /inference="COORDINATES: protein motif:HMM:NF039936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG73192.1"
                     /translation="NSSNNLNVSLKGNFTNNGTYRPQLNTTTFNGGTQSILGTSTTSF
                     YNLVANPGTSLTMGSSSINVDGNLTLSSGQLLCGSYSVNLKGNFTNNADYTDNGTGVR
                     LVGATQQNISGSGTFSTLELNNTSGARLLSSITLNKDLKLTQGIFNINQYKLTLGVNS
                     NITGTSFSAAKMIEVDGAFSNVGILKYFPIYNGADVTFTYPLGVSSKYTPAVFTYSKN
                     DVVGYIRVNAINSYHPGVLDRNRVLQYYWEVENSAVSGVNGKMEFYYKDADVRGSEPD
                     YVAARTIGATSWSKAAPGPTTDNVDEGTNKITFTYSGANDLNGEYTAGEDTAFPNNIP
                     QFTSQKDGDWNDKNVWVQTGGDPYTLTGAPNGFIVTISAADTVTLNENSAFTYRMQIN
                     GTLKAVQPYYGHNLGTVSGSGTLYLDKGTLPAGRYTQFLSCATGGTLEYGGSTDYNIN
                     ADLYSSVPKIHFTGTGTRVLPSKDLTICHQLLINGPTLDNSVNNRKLTIQGTMERPSG
                     TFTAGTGANAIVEFAGSSAQTLGGTTGNFTGTNKFNHFTINNSSNLSIGANGQVEVGG
                     NLNLTSGRIITTSTNKLSIVNTAINCVNPAGGSASSYVDGPLTKKVNSGDGFKFPVGK
                     GSTVGNKITLLSSQSGTIDWTVEFFTPNSTFGSYAAPLTYVNSKEYWTVSAASGSQAK
                     VGLDWDPASDLTPLMTESGLSDMRVAYDNGGTWTELASSASGDGSNGTVSTSSRYTIP
                     ASGTEEFTIACINTIKPRARFSPSGPVCGTSGIPVTFIGASIPFNYILSYKKDEVAQT
                     PVTITSVPYTLPTSATGATYVLTGFSYNNPSSPTNGVVDPTPVTTYTVPTTANAGPDQ
                     SICGGTSATLAANTPTVGTGLWSILAGAGGTVVTPTVPTSTFNGTNGTSYTLQWTISN
                     GGCTSSDDVGIAFPLLPEQPAAFIHFDDSVCQGDLNVAYSVANNPTLTYTWGYSGSGA
                     TMVGSGNEISIDYSGTATSGNVSVYTTNGCGNSAPLTRAVTVNGRPTASIAVDAGFTP
                     ICDGDNTQMTITLSGGTSPYSFSITDGTNTENFTGATSPFSYIPATHPIWTGPGTNNT
                     YTYSIPTVTSANGCSYHGSNTADVTVYKVPETGPEYHIPNNFGF"
     gene            3685..4131
                     /locus_tag="PK839_10435"
     CDS             3685..4131
                     /locus_tag="PK839_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG73193.1"
                     /translation="MSSLPEKRIVDFISEHHVLTLATSFEEEPWCASCFYVYMPEDNC
                     FVFTSDFNTRHIQQASHNIYVAGNIVLETSVVGKVQGVQFQGIISQPQNEQYEKAKKA
                     YLKRFPVAMLMDTHIWVVDVTYIKMTDNRLGFGKKLIWSKDIALQK"
BASE COUNT         1102 a         1211 c          982 g          892 t
ORIGIN      
        1 caactccagc aacaacctca acgtgtcact aaagggtaat tttaccaaca acggtaccta
       61 ccgcccccaa ttaaatacca ccaccttcaa tggcggaacc cagagcatac tcggaacctc
      121 cactaccagc ttctacaacc ttgttgccaa cccgggtacc tcgcttacca tggggagtag
      181 tagcatcaac gttgacggga accttaccct ttcgtcaggc cagctgctct gcggtagcta
      241 ctccgtaaac ctgaagggaa actttaccaa caacgccgac tacaccgata acggcacagg
      301 tgtaaggctg gttggcgcta cccagcagaa catcagcgga agcggaacat tcagcacttt
      361 agagctgaat aacacgagtg gagcgcggct gctcagctcc atcaccctga ataaagacct
      421 gaagctcacc caggggattt tcaacatcaa ccagtacaag cttacccttg gggtaaacag
      481 caacataacc ggaacgtcgt ttagcgctgc caaaatgatt gaggttgacg gggcattcag
      541 caacgtgggc atacttaagt acttccccat ttataacggg gctgatgtaa cctttaccta
      601 cccgctgggc gtgagcagca agtacacacc tgctgtattc acctactcaa aaaacgatgt
      661 ggtaggctac atcagggtaa acgccatcaa cagctaccat cccggcgtgc tggacaggaa
      721 cagggtgctg cagtactact gggaggttga aaactcggct gtttcggggg taaatggaaa
      781 gatggaattc tactacaagg atgcagacgt taggggaagc gaacccgact atgtggctgc
      841 ccgtacaatt ggggcaacca gctggagcaa ggctgctccg ggccccacta ccgacaatgt
      901 ggatgaaggc acgaacaaaa taaccttcac ctactctggt gccaacgacc tgaacggcga
      961 gtacaccgca ggtgaggata cggcattccc caacaacatt ccacagttca cctcgcagaa
     1021 ggacggtgat tggaacgata agaatgtttg ggtgcaaacc ggcggcgatc catataccct
     1081 aaccggtgcg cccaacggct tcattgttac cattagcgca gccgacaccg ttaccctgaa
     1141 cgagaattcc gctttcacct acaggatgca gataaacggc accctaaagg cagttcaacc
     1201 ctactacggg cataacctgg gaacggttag cggctcagga acgctctacc ttgataaggg
     1261 caccctgcct gcaggccgct acacccagtt cctcagctgc gccacaggcg gtacgctgga
     1321 gtacggcggt agcacggact acaacattaa cgccgacctc tacagctcgg ttcctaaaat
     1381 tcactttacc ggaacgggta cccgggtact tcccagcaag gatctgacca tttgccacca
     1441 gctgcttatc aacggcccca cactcgataa cagcgtaaac aaccgcaagc ttaccataca
     1501 gggaaccatg gagcgccctt caggtacatt caccgccggc accggtgcaa atgccattgt
     1561 ggagtttgcc ggctcgtcgg ctcaaaccct tggcggaacc accggcaact tcaccggcac
     1621 aaacaagttc aaccacttca ccataaataa ctccagcaac ctctccattg gcgcaaacgg
     1681 ccaggtggag gttggcggta acctgaacct caccagcggc cggattatta ccacctccac
     1741 caacaagctc agcattgtaa atacagctat caactgtgta aaccccgcag gaggctcagc
     1801 atcatcgtat gttgacgggc cgttgactaa aaaggttaac tcaggcgacg gcttcaaatt
     1861 tccagtggga aagggcagca cggtgggcaa caagataacc ctcttgtcgt cgcagagcgg
     1921 aaccattgat tggacggttg aattcttcac ccccaactcc acctttggct cctacgcggc
     1981 gccgcttacc tacgtgaact ccaaggagta ctggacggta tcggctgctt cgggtagcca
     2041 ggccaaagtg gggctcgact gggatcccgc cagcgacctt accccgctga tgaccgaaag
     2101 cggcctgtcg gacatgaggg ttgcctacga caatggggga acatggacag agctagcatc
     2161 aagtgcaagt ggggatggca gcaacggcac cgtttctacc tcaagcaggt acaccattcc
     2221 cgcttcgggc acggaggaat tcaccattgc ctgcataaat accattaagc caagggccag
     2281 gtttagtcca tcgggaccgg tttgtggcac ttccggcatt ccggttacct ttattggggc
     2341 ctccataccc ttcaactaca ttcttagcta taaaaaggat gaggttgccc aaactccggt
     2401 aaccattaca tcggttccct ataccctgcc taccagtgca acgggggcaa cttacgttct
     2461 taccggtttc tcctacaaca acccctcgag cccgaccaac ggtgtggttg acccaacgcc
     2521 cgttacaacc tacaccgttc ccaccaccgc taatgccggt cccgaccagt caatttgcgg
     2581 tggaacatct gcaaccctgg cagccaatac cccaaccgta ggaacaggtc tttggagtat
     2641 cctagccggt gcgggaggaa cggtggttac cccaacagtt ccaaccagca cctttaatgg
     2701 aaccaacggc accagctaca ccctgcagtg gaccatctcc aacggcgggt gcacctcgtc
     2761 cgacgatgtt ggcatagcat tcccgctgct ccccgagcaa ccggctgctt tcattcattt
     2821 tgatgacagt gtttgccagg gtgatttgaa cgtggcctac tcggtggcca acaaccccac
     2881 ccttacctac acctggggct atagcggttc cggagccacc atggtgggct cgggcaacga
     2941 aatctccatt gactactcgg gtaccgccac ctcggggaat gttagcgttt acaccaccaa
     3001 cggttgcggc aatagcgccc cgctaaccag ggcagttacg gttaacggca ggccaacggc
     3061 atccattgct gtggatgcag gctttacccc catctgcgac ggcgataaca cgcagatgac
     3121 catcacttta tcggggggta cttcgcccta cagcttcagc atcaccgatg gcaccaacac
     3181 ggagaacttt accggcgcca catcaccgtt cagctatatc cccgcaacac acccgatttg
     3241 gacagggccg ggtaccaaca acacctacac ctactccata cccaccgtta cctcggccaa
     3301 tggctgctcg taccatggct ccaacaccgc cgacgtaacc gtttacaagg tcccggaaac
     3361 aggacccgag taccatattc ccaacaactt cggcttttaa cttcaatatt accgctaaaa
     3421 caaagactac cacagcatta ctcaatgtag tgttgtggca gtctcttttt aacagtgttg
     3481 gcggtagcca aaatcggttg tctaatacca agttgcgttc ttatatgaaa ataggtgaaa
     3541 agttcaaaaa atgatggtac tttgtgctct ctgtgcctta gttgtgtcct ttgtggttaa
     3601 ctataatatt gtacctatga atgcaacatg gcataaagcg taagtgaaac atttttatac
     3661 ttttgtacca gatataaaca cctaatgagc agccttcccg aaaagcgtat tgttgatttc
     3721 atcagcgagc accatgttct caccctggcc accagctttg aggaggaacc gtggtgcgcc
     3781 agctgctttt acgtctatat gcctgaggat aactgcttcg tcttcacctc cgactttaac
     3841 acccggcaca tccagcaggc tagccacaac atctatgttg ccgggaatat tgttttggaa
     3901 accagcgtgg tgggaaaggt tcagggagtt caattccagg gaatcatcag ccagccccaa
     3961 aacgagcagt acgaaaaagc taaaaaagcc tacctgaaac gctttcccgt ggcaatgtta
     4021 atggataccc acatctgggt ggtggatgtt acctatatta aaatgaccga caaccgcctt
     4081 gggttcggga aaaagctgat atggtcgaag gatattgcac ttcaaaagta aatttcttca
     4141 aggtgaaaat tcaatcccaa accctccaaa atcaggtaat gtaattt
//
LOCUS       DAOSHM010000119         4112 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_19702_length_4112_cov_16.154794, whole genome shotgun
            sequence.
ACCESSION   DAOSHM010000119 DAOSHM010000000
VERSION     DAOSHM010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 4112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4112
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19702_length_4112_cov_16.154794"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(12..1979)
                     /locus_tag="PK839_10440"
     CDS             complement(12..1979)
                     /locus_tag="PK839_10440"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HOG73194.1"
                     /translation="MKRFFLTLIISGIGTCYSQNISVESFRLLEKDLDARVNFPKRDQ
                     NGEVCAIIKVVTPETDFVWDSDALGIVSAERKTGEYWLYVPRGARFLTIKHDLLGILR
                     EYQYPIPIQSATVYEMKLITAEIEKIVKKPEIITQWLAIDSEPKGANIFIEEKLVGTT
                     PYTGQFPEGEYTYRIELPRYHPEAGKVTLKGKRETLTFTMRPRFGKISVTSSPESGMM
                     IYLNDENTGKTTPATLEGVSSGNQTLKLTSQWYQPQAKTVTVNDNQTTTVSFTMEPAF
                     ANITVKTTPPADILINGTKKANSTYSDRMLTGIYTLKAELDKHYPEERQLVVEAGKAQ
                     NISLDLKPKTGRIDITSTPFDANIKLNGKDYGTTPTTVRDLLVGSYTLTLEKPGYGTV
                     NKTVSITEGQTTEINETLPTGMEVTIASTPSGAQLWVNGASVGTTPFKTTLAFSSHSV
                     KLVNGKKEVNETITVTQGGKTRWEFDVAEFPSTVTFTDPRDQKTYKYVTIGKQVWMAE
                     NLAYKPSNGNYWAYDNDEGNVAKYGYLYDWQTAKNVCPTGWHLPSDAEWDQLTGFVGS
                     NKGTKLKAKSGWSSNGNGTDDYGFSALPGGYRYANGTFGYIGYEGYWWSSTEYGPDIV
                     GTRYMRYNYGTVSRSHLSKSWGFSVRCVRDL"
     gene            complement(2036..3826)
                     /locus_tag="PK839_10445"
     CDS             complement(2036..3826)
                     /locus_tag="PK839_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007479626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG73195.1"
                     /translation="MKQVHKNSSKGLFVTLVMVLISLNVSGQTLDQIKADRHTYIWGE
                     GNGTTLNSADQEALGMLSNQISVQVESRYDLLRQEVTGSGKQTFEEVFKGVINTYSSA
                     TLRNTERIVISNEPDAKVFRYIKRDEIDKIFSDRERKIVEFARNGEQAALSGRVADAL
                     RYYYWSLTLLRSHPNANTIGMKDEKGNDRLLISWLPMQINTVLADVRVTIAGTNRMDD
                     LTRYFLNIRYQNEPARNFDYSYWTGRDWTNIYSAKDGIGIAEFFGKSDINEIRIKAEY
                     VFEGETAIDNELRDVMAKLSVVPFRGSYFSVPVGKVEEPISLPETAGGVTMVQDTKPY
                     EEKMNSLLNSIRTQNYQAAQSLFTPEGYEMYKSLLQYGQARIVKEPEFKFIHFEKVVM
                     CRALTMSFHFKNNNRNFIEDVVFQFNEEKKISNISFGLEQKAINDIIGKDVWDERVRL
                     ILINFLENYKTAYALKRADYIESLFADDALIIVGSVLKVKATGDNPYLNNNIVKLNRY
                     TKEQFIRNLRHSFASNEFINIRFEDNTVRKSGKGGEVYGIEIKQDYFSSNYGDSGYLF
                     LMVDFNDANQPVIHVRTWHPEKNFSLSDFL"
BASE COUNT          971 a          934 c          948 g         1259 t
ORIGIN      
        1 agtcaaataa cctataaatc cctcacgcaa cgaacagaaa aaccccaact cttactgagg
       61 tggctcctgc tcacagtgcc gtaattgtag cgcatgtagc gggtcccgac gatatcggga
      121 ccgtactcgg tagaactcca ccagtaaccc tcgtatccaa tgtagccgaa agtaccattg
      181 gcgtaacggt agccccccgg aagagccgaa aagccgtagt cgtcggtgcc gtttccgttg
      241 gagctccacc cgcttttggc ttttagcttg gtgcccttat tgcttcccac aaaaccggtt
      301 agctggtccc actccgcatc gctgggcagg tgccagcccg tggggcaaac attttttgcg
      361 gtttgccagt cgtataaata accgtatttt gcaacattgc cttcgtcgtt atcatatgcc
      421 cagtaattac cattactggg tttatatgcc agattctcgg ccatccaaac ctgtttgcca
      481 atggttacgt acttataggt tttttggtcg cgggggtcag tgaatgtaac agttgaagga
      541 aattcagcca catcaaattc ccaccgtgtt tttccacctt gcgttacggt aatggtttcg
      601 tttacctcct ttttgccgtt taccagctta acgctatggc tgctaaaggc aagcgttgtt
      661 ttaaaaggtg ttgtaccaac agaagccccg tttacccata gctgtgcgcc gctgggggtg
      721 ctggctatgg ttacctccat gccggtgggg agggtttcgt ttatttcggt ggtttgcccc
      781 tcggtaatgc taactgtttt gtttaccgtg ccgtagccgg gcttttcaag cgtaagcgtg
      841 tagctaccta ccagtaaatc cctcacagtt gtaggggtgg tgccgtagtc cttgccgttg
      901 agcttaatgt ttgcatcaaa tggggtactg gtaatatcta tcctcccagt ttttggctta
      961 aggtcgaggg aaatgttctg cgctttccct gcttccacaa ccagctggcg ttcttcgggg
     1021 tagtgcttgt cgagttcggc tttaagggtg tatatgcccg ttagcatacg gtcggaatag
     1081 gtgctatttg cctttttggt gccattaata aggatatcgg cagggggagt ggttttcacg
     1141 gtaatattgg caaacgctgg ctccatggta aaggagacgg tggtggtttg gttatcgtta
     1201 accgtaacgg ttttggcctg cggctggtac cactggctgg taagcttaag ggtttggttt
     1261 ccgctgctta cgccctcaag ggttgcgggg gtggttttac ccgtgttctc atcgttcagg
     1321 tatatcatca tgccactctc ggggcttgag gttacactga ttttcccaaa ccgtgggcgc
     1381 atggtaaagg taagggtttc acgtttccct ttcagggtta ctttcccggc ctcggggtgg
     1441 tagcggggca gctcaatgcg gtaggtgtat tcgccctcgg gaaattggcc tgtgtagggc
     1501 gttgttccaa ccagcttctc ttctataaaa atatttgcac cctttggttc gctgtcaata
     1561 gccagccatt gggtaatgat ttcaggtttt ttcacgatct tttcaatctc agccgtaatg
     1621 agcttcattt cgtaaaccgt tgccgattgg attgggatag ggtactggta ttccctgagt
     1681 attcccagca ggtcgtgctt aattgttaaa aaccttgccc cacggggcac gtaaagccag
     1741 tactcacccg ttttgcgctc ggccgaaaca atacctaaag catcggaatc ccagacaaag
     1801 tctgtttcgg gtgttaccac cttaattatt gcgcacacct cgccgttctg gtcgcgtttg
     1861 gggaagttaa cccttgcatc taaatccttt tctagcaatc gaaatgattc tacggagata
     1921 ttctgcgagt aacaggtacc aattccagag attataaggg ttagaaaaaa acgtttcatt
     1981 gaaaaatatt ttcgttgtta ttcgtaatat gggagttgaa gttaaaagaa aaaatctaca
     2041 gaaagtcgga taagctaaag tttttttcgg gatgccaggt acggacgtgt ataactggct
     2101 ggttggcgtc gttaaaatca accattagga acaggtaccc cgagtcgccg tagttggacg
     2161 agaagtagtc ctgcttaatc tctattccat aaacctcacc gcccttgccc gattttcgaa
     2221 ccgtgttgtc ctcaaaccgg atgttaataa actcgttgct ggcaaaggag tgtctcaggt
     2281 tcctgatgaa ttgctccttg gtgtaacggt taagctttac aatattattg ttaaggtatg
     2341 ggttatcgcc ggtagcctta acctttagca cagaaccaac gatgataagg gcatcatcgg
     2401 caaaaaggct ctcgatgtaa tcggcccgct taagggcgta agccgtttta tagttttcca
     2461 gaaaattaat gagaattagc ctaacccttt cgtcccaaac gtccttccca ataatatcat
     2521 ttatggcctt ttgctctaat ccgaaggaga tatttgatat tttcttctcc tcattaaatt
     2581 gaaaaacaac atcctctata aagttgcggt tgttgttctt aaaatggaag ctcatggtga
     2641 gcgccctaca cataaccact ttttcaaaat ggataaactt aaattcgggc tcttttacta
     2701 tccttgcctg cccgtactgc agaagacttt tatacatctc gtaaccctca ggggtaaaaa
     2761 gtgattgcgc tgcctgatag ttttgggtac ggattgagtt gagcagggag ttcatctttt
     2821 cttcgtaggg tttcgtgtcc tgtaccattg taacacctcc agctgtttct ggcaggctaa
     2881 ttggctcttc aacttttcca acgggcacgc taaagtagct gccacggaag ggaaccacgc
     2941 ttaactttgc cattacatcc ctcagctcat tatcaatggc ggtttcgccc tcgaaaacat
     3001 attcggcctt aatccttatc tcgtttatat ctgattttcc aaaaaactct gcaatgccaa
     3061 taccatcctt tgcgctgtaa atatttgtcc agtccctgcc agtccagtag ctgtagtcga
     3121 aatttcgtgc aggttcgttt tggtagcgaa tatttaaaaa gtaacgggtt aaatcatcca
     3181 tcctgttggt tccggcaatt gttaccctaa catcagcgag aacagtgttt atctgcatgg
     3241 gcagccagga tattagtagc ctgtcattcc ctttctcatc cttcatgcca atagtattgg
     3301 cgttggggtg gctacgaagc agagttagcg accagtagta gtagcgcagg gcatcggcaa
     3361 ccctgccgct gagtgcagct tgttcaccgt tacgtgcaaa ttcaacaatt tttctttccc
     3421 tgtcggagaa tattttgtct atttcgtccc gcttgatata cctgaaaacc ttggcatcag
     3481 gttcgttgct tattacaata cgctccgtat tcctaagcgt tgctgatgag taggtgttta
     3541 tcaccccttt gaaaacttct tcaaaggttt gtttacccga acccgttact tcctgtcgca
     3601 gtaggtcgta tcggctctca acctgaacag aaatctgatt ggaaagcatt cccaaagctt
     3661 cctggtcggc gctgttgagg gttgttccat taccttcacc ccaaatatag gtgtggcggt
     3721 cagcctttat ttggtcgagg gtttggcctg aaacattaag agaaattaaa accatcacaa
     3781 gggttacgaa taaacctttc gaactattct tatgtacctg tttcattttc ctggttcgtt
     3841 ggtttaattt ttagaaagca ccacaatata aatatttttt tcagcatgtt caatcagcgt
     3901 tgtaaaaatg aagccttttt ttgcagcaaa gcggctgcct gtgtggtggg gctgcaaaat
     3961 gtggcttaat gtgggagggg aatttaaaat ttttactaat atgtgacggg caattaaaat
     4021 ggtaaaatat attattgttt ttcaaagagc taaattattc aatttcaaag cgtaataacc
     4081 ttttccgttt cgtcatacac ttgttgccaa cg
//
LOCUS       DAOSHM010000120         3841 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_21799_length_3841_cov_8.812203, whole genome shotgun sequence.
ACCESSION   DAOSHM010000120 DAOSHM010000000
VERSION     DAOSHM010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3841
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21799_length_3841_cov_8.812203"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     regulatory      121..227
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            647..1951
                     /locus_tag="PK839_10450"
     CDS             647..1951
                     /locus_tag="PK839_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HOG73196.1"
                     /translation="MERDYQFETLQVHAGHRPDRDTLSRAVPIYQTTSYQFCSSDHGA
                     NLFELKEPGYVYTRMGNPTTDVLEKRIAALENGKAAVATSSGHAAQFLALSNILNAGD
                     NFVSSPFLYGGSYNQFNVALRRLGVDVKFSKSNNTEDFKALVDERTKALYVETIGNPS
                     FCIPDFENFAKLADRHGIPLVVDNTFGGGGWLCRPIDHGANVVVESLTKWVGGHGTSM
                     GGAVIDAGNFNWGNGNFPQFSEPSEGYHGLVFWKEFGENSPGGNTAFAVRARVEGLRD
                     FGATISPFNSFLMLQGIETLSIRMERHCQNTLLLAQWLEQHPKVESVCYPGLASNHYH
                     RLALKYLKHGFGGVLSFTIRGNTEQTKRFVDSLKLVSHLANVGDTRTLIIQPSATTHR
                     QLSPSEQLAAGVLPNQLRVSVGIENICDIKDDFEQAFEKLNS"
     gene            1963..3000
                     /gene="metX"
                     /locus_tag="PK839_10455"
     CDS             1963..3000
                     /gene="metX"
                     /locus_tag="PK839_10455"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014776559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004414 - homoserine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HOG73197.1"
                     /translation="MYAAIEGNFRIDTLPLECGQILKQVNIRYHILGDIGRNADKVVW
                     ICHAFTANSNPAEWWPGIVGEGKYFDPDKTPVVCANILGSCYGSTGPKSVNPETNQPY
                     LRNFPLVTFRDVVRAHNLLRIHLGINRIWMLIGGSVGGYQAMEWECMFPGTTQHLVLI
                     ATAAISSPWVKAFSQSQRLALLADQSFVNLEVDGGRAGLVAARSIALLSYRGRSSYNI
                     TQQDTCASRLEGYRACTYQTYQGEKLANRFDAYSYYTLSLMLDSHNMGRSRAGACEAL
                     ASLRANTLVVGIDSDILFPVEEQIFLARHIPKARYVEIHSDFCHDGFLIENEQLSSAV
                     SSYLSCFGEVV"
     gene            3019..>3841
                     /locus_tag="PK839_10460"
     CDS             3019..>3841
                     /locus_tag="PK839_10460"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HOG73198.1"
                     /translation="MDTTIVRKTNKQAHKSYPITQQVIGLFGFGNVGKGLYHVLCHVP
                     REKAYIKTICVKDRSKKRDECANLITYNPDDILRDPDINLVVELIDSARDAFSIVKKA
                     MLMGKSVVSGNKAMLAHHLPELVQLQEKTGVALLYDASACGSIPVIRNLEEYYDNDLL
                     VSIRGILNGSTNFILSRMFIHSESYSDALCQAQSLGFAESNPDFDVMGFDALYKLIIL
                     ALHSFGTFIQPEQAFCYGIQNLTNFDIGYAREKGKKIKLVAHLRKFSESCFTLFVI"
BASE COUNT         1039 a          822 c          914 g         1066 t
ORIGIN      
        1 tttcgggcat caaaaaaacc aacgaggttg taaaggacga aaatagctga gcgaatattt
       61 gtatttggtt atcaacgtaa atatatttgc accatcttgt ttttacttct aaaagcatcg
      121 gaattataga gaagagctga gagaacaggc tcattgaagc tctagcaacc tgcatttaag
      181 ttgtaaggtg ctaaaacctg actcctgtgg aggagtaatt ataactctta acttaacgcc
      241 atgtgtgcca ttaaaatttt atcgtttcct ctctcttcgg actacttaaa tagttcgcgt
      301 tggctgatat gctctcctag caagttaacc ccagccatgc gaatgcgccg ctaagcgcgg
      361 taaaaaatcc aattttgatt ccctcaaagg gatatgcctg ttactatttg gaagtggtta
      421 caggcggtga tatggctacg tatcacccag tcggctttgt atggcttagc ctatatgctg
      481 tccggacaat tcaaaacaac atctgtctat tccttttttc ttgtttattc aagaatttag
      541 gagtgagctc acaaggctca tcgaaagcta ctaaacctag ctagacataa ggtttacgtg
      601 ttgagcttac aatactgtag aattattttt taaaaatcaa ctacttatgg aacgagatta
      661 tcaatttgaa accctgcaag tgcatgccgg tcatcgaccc gacagggata ctttgtctag
      721 ggccgtgccc atatatcaaa caacctcata ccagttttgc agctccgatc atggggctaa
      781 cctttttgag ctgaaagagc ccggttatgt ctatacccga atgggaaacc caacaaccga
      841 tgtgctggaa aagcgaattg ctgcacttga aaatgggaaa gctgctgttg ctacttcttc
      901 tggccatgct gctcagtttt tagcccttag taatatttta aatgctggcg ataacttcgt
      961 gtcgtctcca tttctatacg gaggcagcta caaccagttt aatgttgccc tacgcaggct
     1021 tggagttgat gtaaaattca gcaaatcaaa caatacggaa gattttaagg cgttagttga
     1081 tgagcgtacc aaggcgctgt atgttgaaac aattgggaat cctagctttt gtattcccga
     1141 cttcgaaaat tttgccaaac ttgcagacag gcatgggatt ccgctagttg ttgataatac
     1201 atttggtggg ggaggatggc tatgccggcc aatagaccat ggagcaaacg tagtggttga
     1261 gtcgttaacc aagtgggttg gaggccatgg aaccagcatg ggtggtgcgg tgattgatgc
     1321 aggcaatttc aactggggca acggcaactt tcctcaattt tcagagccct cggagggata
     1381 ccatggtttg gtcttttgga aggagtttgg cgagaatagc cccggcggaa acactgcatt
     1441 tgctgtgagg gcaagagtag aaggtttacg cgatttcgga gcaaccataa gcccttttaa
     1501 ctcctttcta atgcttcaag gtatagaaac tctttccatt cgcatggaaa ggcactgcca
     1561 aaatacccta ctgcttgcac agtggctgga acagcacccc aaagtggaaa gcgtctgcta
     1621 cccgggttta gcatcgaatc attaccatcg ccttgcctta aagtacctga agcacggatt
     1681 tggtggagta ttatctttta caattagggg gaatacggaa caaactaaaa ggtttgttga
     1741 tagtcttaag ctggttagcc acttggctaa tgttggcgac acccgaacac ttatcattca
     1801 gccatcggct acaacccaca ggcagctttc cccttcagaa cagctagctg caggtgtgct
     1861 gcctaaccag cttagggtgt cggttggaat cgaaaacatt tgtgatatta aagacgattt
     1921 cgaacaggct tttgagaagt taaacagcta ataatttttg caatgtacgc ggctattgaa
     1981 gggaacttta gaattgatac tctaccctta gagtgtggtc aaattttaaa gcaagtaaac
     2041 ataaggtatc acatactagg tgacattggt agaaatgccg acaaagttgt ttggatatgc
     2101 catgcattta cggccaacag caatccggcc gagtggtggc ctggaattgt gggcgaaggg
     2161 aaatacttcg accccgataa aacgcctgtg gtttgcgcca atattctagg ctcatgctac
     2221 ggatcaacgg gaccaaaatc ggttaacccg gagacaaacc aaccatacct tcgtaatttt
     2281 ccactggtta cctttcgcga tgttgtccga gcgcacaacc tgcttaggat tcacttgggg
     2341 attaatagga tttggatgtt gattggcggt tcggttggag gttatcaggc aatggagtgg
     2401 gagtgcatgt ttcccggcac aacgcagcac ttggtgctaa tagccacagc agcgatttcg
     2461 tcgccctggg ttaaagcgtt tagccagtcg cagcggcttg cccttttggc cgaccagtcg
     2521 tttgtcaacc ttgaggttga tggtggaaga gcagggttgg ttgctgcccg cagcatcgca
     2581 ttgcttagct ataggggtcg gagcagctac aacattaccc agcaggatac ctgcgcttcc
     2641 cggctcgaag ggtacagagc ctgtacctat caaacctacc agggcgaaaa gctggccaac
     2701 cgtttcgatg catactcata ctatacgttg agccttatgc tcgattccca taatatgggc
     2761 agaagccgag ctggagcttg cgaggcactc gcttctttac gggcaaatac gctggttgtg
     2821 ggcatagatt ccgatatcct tttcccggtt gaggagcaaa tttttcttgc caggcatatt
     2881 ccaaaagccc gttacgtaga aatacattct gatttttgtc atgatggttt tttaattgag
     2941 aatgagcagc tttccagtgc cgtaagcagc tatctgagct gttttggcga ggtggtctag
     3001 ctaaaaattt gagttgaaat ggatacaact atagttagga aaactaataa acaggcccat
     3061 aagagctatc ctataaccca acaggttatt ggcctatttg gatttgggaa tgtgggcaag
     3121 gggctatacc atgtgctttg ccacgttcca cgcgaaaaag cctacatcaa aacaatttgc
     3181 gtgaaggata ggagcaagaa gcgggatgaa tgtgccaacc tgattaccta taatcctgat
     3241 gatattctcc gcgatcccga tattaacctg gtggtagagc tgattgacag tgcccgggat
     3301 gctttcagta tagttaaaaa agccatgctg atgggaaaaa gtgttgtatc gggaaataag
     3361 gctatgcttg cacatcacct tcccgagctg gtgcagcttc aggaaaaaac aggagtagcc
     3421 ctgctttacg atgcttctgc atgcggtagc attcctgtca tacgcaatct tgaggagtac
     3481 tacgacaacg atttgcttgt ttccataagg ggcattttga atggatcaac caacttcatc
     3541 ttgtcaagga tgtttatcca ttctgagtca tactccgatg ccctttgcca agctcaatcg
     3601 ctcggatttg ccgaaagcaa ccccgacttc gatgtgatgg gttttgatgc gctctataag
     3661 cttatcatac tagctttaca tagctttgga acatttattc aaccggaaca ggccttttgt
     3721 tacggaatcc aaaatttaac caatttcgac attgggtatg cccgggaaaa gggcaaaaag
     3781 ataaagttgg ttgcccattt gcgaaaattt tccgaaagct gcttcacgct ttttgtaatc
     3841 c
//
LOCUS       DAOSHM010000121         3840 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_21805_length_3840_cov_23.289564, whole genome shotgun
            sequence.
ACCESSION   DAOSHM010000121 DAOSHM010000000
VERSION     DAOSHM010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3840
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21805_length_3840_cov_23.289564"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(147..890)
                     /locus_tag="PK839_10465"
     CDS             complement(147..890)
                     /locus_tag="PK839_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HOG73199.1"
                     /translation="MNIKLKIWRQKNAQSKGGFETYLLDGVSPEMSFLEMLDYLNSRL
                     IIEGKSPVAFDFDCREGICGSCGIYINGRPHGSDRKTTTCELRMRKFKDGETITLEPW
                     RAGPFPVIKDLMVDRSAFDKIIQAGGYITVNAGAAPEANSILVPKKNADTAFDTASCI
                     GCGACVAACKNASAMLFVAAKITHLAQFPQGRIEAPERVKRMVAKMEELGFGGCTNTR
                     ACEAECPKGISITAIAKLNRDYISAKMKD"
     gene            complement(924..2843)
                     /locus_tag="PK839_10470"
     CDS             complement(924..2843)
                     /locus_tag="PK839_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HOG73200.1"
                     /translation="MTKLDAKIPEGPLADKWTSHKAKLRLVNPANRKKIDIIVVGTGL
                     AGSSAASTLGELGYNVKVFCYQDSPRRAHSVAAQGGINAAKNYKNDNDSVYRLFYDMI
                     KGGDFRAREANVYRAAEVSNLVIDHYTALGVPFARDYGGHIDNRSFGGVQVSRTFYAK
                     GQSGQQCLIGAYSDLSRQISMGTVTMYPRREMMDLVLVDGKARGIVVRNLITGEIERH
                     SAHAVVLATGGYGTIYYLSTLALNSNGSAAWKAYKKGAYFANPSFVQIHPTSIPVLND
                     YQCKLTLMSESLRNDGRVWVPKQKGDTRKPNDIPEEDRDYYLERRYPAFGNLVPRDVA
                     SRAAKERCDAGYGVGPTGLSVYLDFRDAISRKGKKYIEDNYGNLFEMYEKISGESPYS
                     VPMRIYPAGHYTMGGLWVDYNLMTTVPGLFAIGESNFSDHGANRLGASSLMQTSGDGY
                     FILPYTISDYLADEIRTPKISINIPEFDEAENTVRERINKLLGINGTETSTSFHKRLG
                     KIMWDYCGMVRNEEGLKKALALIDELKDEFWHNLKLPDSSNEVNHELEKACRVADFLE
                     LSKLLVYDALNRRESCGAHFREESQTETGEALRDDEHFSYVAAWEFAGDSKEPILHKE
                     ELKFEYVKLQERSYK"
     gene            complement(2866..3537)
                     /locus_tag="PK839_10475"
     CDS             complement(2866..3537)
                     /locus_tag="PK839_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HOG73201.1"
                     /translation="MMGIFSSSIGKKLIMSITGIFLMIFLLVHLSVNLTLLVGDGETF
                     NHAAHFMGTNPLVKIIEPILAAGFILHIFFATYITLKNRSTRPVGYTQTSRNGVSTWP
                     SKNMYILGGLILVFLVIHLSNFFWKMRYGEMPTITYNGLVMDDAYSLVADLFIKYWWY
                     DLLYLAAAVLLGLHLSHGFWAAFQTVGWNGTKWLKRLEVIAYIYAVIIGIGFSVIPLY
                     FLIFK"
BASE COUNT         1077 a          909 c          791 g         1063 t
ORIGIN      
        1 tgagctaatc ccgcaaatat gaaagtttaa agcctgataa tttgtcttaa taattactga
       61 aattctgcgc aaaggtaatt ttttatcgca tttcatcaac ctattttttc ttaatagttt
      121 accataagcc caaaatacca ataaaatcaa tctttcattt tggctgaaat gtaatccctg
      181 tttagcttgg caatggcagt aatagagatt cctttggggc attctgcttc acatgcacga
      241 gtatttgtgc agccgccaaa gcccagctct tccatttttg caaccattcg ctttaccctt
      301 tcaggagcct caatcctacc ctgaggaaac tgggctaaat gagtaatctt agcagcaaca
      361 aacagcatgg ctgaagcatt cttacaggct gcaacacaag ccccgcatcc aatacatgag
      421 gcggtatcaa aagcagtatc tgcgttcttt ttgggaacta gtatgctgtt tgcctctggc
      481 gcagcaccag cgttaacggt tatatacccg ccggcctgaa taattttgtc gaatgctgac
      541 ctgtcaacca tcaggtcttt gataaccgga aaaggtcctg cacgccaagg ttcaagggtt
      601 attgtttcac catccttgaa ttttcgcata cgcaactcgc aagttgtggt ttttctgtcg
      661 ctcccgtggg gccttccgtt aatatatatt ccgcaagaac cacaaatgcc ctccctacag
      721 tcaaaatcga atgcaacagg gcttttaccc tctataatta agcggctatt caagtagtct
      781 aacatctcca aaaatgacat ttcaggagaa acaccgtcaa gcaggtacgt ttcaaaacct
      841 cccttgcttt gggcattttt ttgcctccat attttaagct tgatattcat ttgtcttaat
      901 agttaaaaca acgtaaatat ttactactta tagcttcgtt cctgaagttt aacgtactcg
      961 aatttcaact cctccttgtg gagaattggc tctttactgt cgcccgcgaa ctcccaagca
     1021 gcgacatacg aaaaatgctc atcatcgcgc aaggcctcgc ccgtttcggt ttgactttcc
     1081 tccctgaagt gtgcaccaca actttcccta cggttaagcg catcgtaaac tagcagtttt
     1141 gacaactcca gaaaatcggc caccctacat gccttttcaa gttcgtggtt gacctcattg
     1201 gaagaatcag gtagtttaag gttatgccaa aattcatctt ttagctcatc aattaatgct
     1261 aaggcttttt ttaaaccctc ctcattacga accattccac agtagtccca cataatctta
     1321 cccaggcgct tatggaacga agtggatgtt tcggttccat ttataccaag cagcttgtta
     1381 atgcgttcac gaacagtatt ttccgcctcg tcgaactcag gaatgttaat tgaaattttg
     1441 ggagtacgaa tttcatcggc aaggtagtcg gaaatggtat aaggaagaat aaagtacccg
     1501 tcgccagagg tctgcatcaa ggaacttgca ccgaggcggt ttgctccgtg atcggaaaag
     1561 ttggattcgc caatggcgaa caagccgggc acggttgtca tcaggttata atcaacccaa
     1621 agccccccca tggtgtagtg gcctgccggg taaattcgca tgggaacaga gtatggcgac
     1681 tctcctgaaa ttttttcata catttcgaag aggttaccat agttatcctc gatgtacttc
     1741 ttccctttcc tgctaatggc atcacgaaaa tcaaggtaaa cagacaaacc ggtaggacca
     1801 acaccatacc ccgcatcgca gcgctccttt gcggcacgcg aagctacatc cctaggaacc
     1861 agattgccaa aagccggata ccgacgttcc aggtagtaat ccctatcttc ctcaggaata
     1921 tcgttaggtt tgcgggtgtc gcctttttgc ttcggaaccc aaactctacc atcgttgcgg
     1981 agcgattccg acattaacgt tagcttgcac tggtagtcgt tcagcacagg aatgctggtt
     2041 ggatgaattt gaacaaaact ggggttagca aaatacgcac cctttttgta agctttccag
     2101 gcggcagaac catttgaatt tagcgctaat gttgataggt agtagattgt gccatatcca
     2161 ccggtggcca aaacaacggc atgagcggaa tggcgctcaa tttcgccggt gataagattg
     2221 cgaacaacaa tacctcgcgc cttgccatca actaaaacca aatccatcat ctcgcggcga
     2281 ggatacattg taactgtgcc catgctaatc tgccgcgata ggtcagagta agcgccaatc
     2341 aaacattgtt gaccactttg ccctttggcg tagaatgttc tggacacctg gacgccccca
     2401 aatgaacgat tatctatatg tccaccatag tcccttgcaa acggaactcc cagcgcagta
     2461 taatggtcaa ttaccaggtt actaacctct gcggcacggt aaacattggc ctcgcgggca
     2521 cggaaatcgc ctcccttaat catatcgtag aaaagacgat aaacgctatc gttatcgttc
     2581 ttgtaattct ttgcagcatt tataccaccc tgagcagcca cggagtgtgc tctacgtggg
     2641 ctatcctgat agcagaaaac tttaacgttg tagcccaact cgcccagagt tgaggctgcc
     2701 gacgagccag ccaacccagt tccaacaacg ataatatcaa tcttcttacg gtttgcgggg
     2761 ttcacaaggc gtaacttcgc cttatgactt gtccatttat cggccagtgg accttcaggt
     2821 atttttgcat cgagcttagt catatatagt atatgttagc tgtgattact tgaaaattaa
     2881 gaagtagaga ggaattacgc taaaacctat accgattata accgcataga tataggcaat
     2941 cacctctaac cgcttaagcc atttagttcc attccagcct acggtttgga aagcagccca
     3001 aaaaccgtgg ctaaggtgca accctaggag tactgctgcc gctaagtaaa gcaagtcgta
     3061 ccaccagtat tttataaaaa gatcggccac aagggagtaa gcatcatcca ttaccagacc
     3121 gttataggta attgtgggca tttcgccata tcgcatcttc cagaaaaagt tggaaaggtg
     3181 aataaccagg aatactaaaa tcaacccacc caaaatgtac atattttttg atggccatgt
     3241 ggaaactcca ttccttgaag tttgggtata accaaccgga cgggtgctcc tgttctttaa
     3301 tgtgatgtaa gtagcaaaga agatgtggag aataaaacct gcggccagaa ttggctcgat
     3361 aattttgact aaagggttgg tgcccataaa gtgggcagca tggttaaatg tttctccgtc
     3421 tcctacaagt agcgtcaggt ttacggagag gtgaacaagc aggaatatca tcaggaaaat
     3481 ccctgtaata ctcattatca actttttccc aatggacgag ctgaaaattc ccatcatggc
     3541 attaaagttt aattttggtg aacaagttag aaaaagttct taaataataa aatacatggc
     3601 cgtacgcaaa agcttataaa tttgaaattt cagataccac cgatttatcc ttcccaatgg
     3661 ccagcgctat agctttttga ctgcttccct gttgcatcac ggcctaaatg gtgtaccttt
     3721 gcgctacggt taagtgcttc atctttgtaa ctttttgagg gaagggagca aagtaaggca
     3781 tttactccgt caggacaagg ggggaaaaaa tgcttttttc tcctttgttt cttgaaaaag
//
LOCUS       DAOSHM010000122         3818 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_21997_length_3818_cov_20.588892, whole genome shotgun
            sequence.
ACCESSION   DAOSHM010000122 DAOSHM010000000
VERSION     DAOSHM010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3818
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21997_length_3818_cov_20.588892"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(103..1422)
                     /gene="rseP"
                     /locus_tag="PK839_10480"
     CDS             complement(103..1422)
                     /gene="rseP"
                     /locus_tag="PK839_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HOG73202.1"
                     /translation="MEILVKVGQFFLSLSILVILHEMGHFMFAKLFKTRVEKFYLFFN
                     PWFSLFKFKHGETEYGIGWLPLGGYVKISGMIDESLDKEQMKQPPQPYEFRSKPAWQR
                     FLIMIAGVMVNVVLAIVIYCATLYTWGEEYLPTRSLKYGVSCDSLAQSIGFQNGDKIL
                     SVSGREVENFQMVAHDILLNDDRTVEVLRGNDTIKITVGEEALASLVKNPLIFIPRIP
                     FIVDEVRPNEPAQAAGFLKGDKLLAVNNTPKQFFDEFRQAMQENKSKTVTVTVERGGQ
                     PVNLEVNVPESGIIGIVPVTDLTKFFEINRKDYTLLQSIPAGIARGTATIKSYLKQLK
                     LIFKPETKAYESLGGFISIGKIFPGTWDWQAFWNLTAFLSIILAIMNILPIPALDGGH
                     VLFLLYEMVSGRKPSDKFLEYAQIIGITLLLLLIVYANANDIVKLFR"
     gene            complement(1422..2579)
                     /locus_tag="PK839_10485"
     CDS             complement(1422..2579)
                     /locus_tag="PK839_10485"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005933297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HOG73203.1"
                     /translation="MKNVAILGSTGSIGTQALQVIESNPELFSVELLTAQNNWKLLVS
                     QALKYQPNAVVIGNDKYYVNVKSALEKTDIKIFAGKNSIADCVGSAQVDMVLVALVGF
                     AGLEPTISAIKAGKTIALANKETLVVAGELISELAMEHKASVLPVDSEHSAIFQSIVG
                     ERSPIEKIILTASGGPFRNFSLEQLGRVTPSDALQHPNWCMGNKITVDSASLMNKGLE
                     VIEARWLFNLKPSQIEVVVHPQSIVHSMVQFEDGSVKAQLGLPDMRIPIQYALTYPFR
                     VKSDFPRINFGNNLQLTFERPDTVRFPNLALAREALERGGNMPCVLNAANEVAVEGFL
                     EQSIGFMQMSEIIAEVMSKSSYIAYPSLDDYKMTDIETRLKTAELISQNLA"
     gene            complement(2592..3443)
                     /locus_tag="PK839_10490"
     CDS             complement(2592..3443)
                     /locus_tag="PK839_10490"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOG73204.1"
                     /translation="MKRKIKIFRNFRNKYRLSILNDETFEEVWQYRLSRMNVIIAVGG
                     FAIFLIIMVSVLIAFTPLREFIPGYPDGETRKGYVKNALRADSLERVLKQWENYYANI
                     NTILNGGVPLTIESKADTVKPSRTVEYARSKEDSLLRNQIEADQMYNLSFNSSGNTKT
                     NTFFFIPPVKGIVTNRYSPLQNHYGIDLVAAPNEVVLAVAKGTVILADWTIETGYTIA
                     VQHENNFISVYKHNSKLLKRQGDVVGAGDAIAIIGNSGELTTGPHLHFELWQNGNTLD
                     PSKFIHF"
     gene            3733..3805
                     /locus_tag="PK839_10495"
     tRNA            3733..3805
                     /locus_tag="PK839_10495"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3766..3768,aa:Met,seq:cat)
BASE COUNT         1090 a          888 c          794 g         1046 t
ORIGIN      
        1 cctccaaaca gcaggagaat agccaggatt attacaaccc actcccaacc cccaattagc
       61 gctaaaaccg atgctgtcat gttgatatgt atttagtgat ggttacctga aaagcttcac
      121 gatgtcgttt gcatttgcgt aaacaatcaa caggagtaaa agggttatgc caataatttg
      181 ggcatactca agaaacttat cgctaggttt acgcccgctt accatttcgt aaagtaaaaa
      241 caagacatgg cctccgtcta atgcaggaat cggcaaaatg ttcataatgg ccagaataat
      301 tgataaaaag gccgttaggt tccaaaaagc ctgccaatcc caggttcccg ggaaaatttt
      361 ccctattgag ataaaaccgc caagtgattc gtaggccttt gtctcaggct taaaaataag
      421 cttaagctgc ttaaggtagc ttttaattgt ggctgttccc ctggcaatac cggcaggaat
      481 tgattgcaga agagtgtaat ccttacggtt aatctcaaaa aatttcgtta aatcggttac
      541 aggaacaata ccaatgatac ccgattcagg aacattcacc tcaaggttga cgggctgtcc
      601 accacgttca accgtaacgg taacggtttt tgatttattc tcctgcatag cctgacggaa
      661 ctcgtcgaag aactgcttgg gggtgttatt tacagccagc agcttatcgc ccttcaaaaa
      721 tccggcagcc tgtgcaggtt catttggtct tacctcatca acaataaagg gaatacgtgg
      781 tatgaaaatc agggggttct ttaccaggct ggccagcgct tcctccccaa ctgtaatttt
      841 tatggtgtcg tttccacgta aaacctctac cgttcggtcg tcgttgagga gaatatcgtg
      901 ggcaaccatc tggaaatttt caacttctct cccgcttacc gaaagaattt tgtcgccgtt
      961 ctgaaagcca atgctttgag caagggaatc acagctgacg ccgtacttca ggcttcgtgt
     1021 tggaagatac tcctcgcccc aggtatagag cgtagcgcag taaatcacaa tggccagaac
     1081 tacgttcacc attactcctg cgatcattat caaaaaacgc tgccaggcgg gttttgaacg
     1141 gaactcgtag ggttgaggcg gctgcttcat ctgctccttg tctagcgact catcaatcat
     1201 ccccgagatc ttaacgtagc cacccaatgg tagccaacct attccatact ccgtttcgcc
     1261 gtgcttaaac ttaaaaagcg agaaccatgg gttaaaaaac aggtagaact tctccacacg
     1321 ggttttgaag agcttggcaa acataaaatg ccccatctcg tggagaatga ctaaaattga
     1381 aaggcttaaa aagaattgtc caactttaac cagtatttcc attatgcaag gttttgtgag
     1441 attagttctg ccgttttcaa gcgggtttca atatctgtca tcttgtaatc gtcaagcgat
     1501 ggatatgcaa tatagctaga ttttgacata acttcagcaa ttatttcgga catctgcatg
     1561 aaaccgatgc tctgctcaag gaaaccctcc acggccacct cgttagcagc atttaacaca
     1621 caaggcatat tacctccacg ttccaaagcc tccctggcca gcgccaaatt aggaaaacga
     1681 acagtatcgg gacgctcaaa agtcagctga aggttgttgc caaagttaat ccgggggaag
     1741 tccgatttaa cccgaaaagg ataggtaagg gcatactgta tagggattcg catatcgggc
     1801 aacccgagct gagctttcac cgagccatcc tcaaactgaa ccatggagtg gacaattgat
     1861 tgaggatgaa ctaccacttc aatctggctg ggttttaggt tgaagagcca cctggcctca
     1921 atcacctcaa gccccttatt catcagtgag gctgaatcaa cagtgatctt gttgcccatg
     1981 caccagtttg gatgctgcag cgcatcgctt ggagtaaccc tcccaagctg ctccagggag
     2041 aaattcctga atgggccacc tgaagcggtg agtataattt tctcgattgg gctacgttca
     2101 cccacaattg actgaaaaat agccgaatgc tccgaatcga ctggtaaaac tgatgcttta
     2161 tgctccatgg ccaattcaga aatcagctcc cctgccacca ccagagtttc cttattggct
     2221 agggcaattg ttttaccagc ttttatggct gaaattgtag gttccaaacc agcaaagcct
     2281 acaagagcca caagcaccat gtcaacttgt gccgacccaa cacaatctgc aatagagttt
     2341 ttccccgcaa aaatcttaat atccgtcttt tcgagagcgc tcttaacatt tacgtagtac
     2401 ttatcattac caattactac ggcattaggt tgatacttta gagcctgtga aacaagaagt
     2461 ttccagttat tttgagctgt aagcagttca acagaaaaaa gctccggatt agattcaata
     2521 acctgcaacg cctgggttcc aatggagcct gtagaaccaa gaatagcaac attcttcatc
     2581 ggttcagctc cttaaaaatg gataaacttt gaaggatcga gcgtgttacc attttgccac
     2641 agctcaaagt gtaagtgggg gccggtggtc aactcgcccg agttaccaat tatggcaatg
     2701 gcatcgcctg cacctaccac atcgccctgc cgcttaagca gcttgctgtt gtgcttgtaa
     2761 accgatataa agttattttc atgctgaaca gcaatagtat agccggtttc aatagtccaa
     2821 tcggccaaaa ttacagtccc cttggctacc gccagaacca cctcattagg agcagccact
     2881 aaatctatcc cgtaatggtt ctgcagagga ctgtaacggt tggtaacaat accctttact
     2941 gggggtatga aaaagaaagt atttgtttta gtgtttccgg aactgttaaa tgataggtta
     3001 tacatctgat cagcttcaat ttgattcctc agcaacgaat cctccttcga tctggcatat
     3061 tctactgttc gactaggttt aactgtatct gccttgcttt caatagtaag gggtaccccc
     3121 ccgttaagta tggtgttgat gttagcatag tagttttccc actgcttgag taccctttcg
     3181 agtgagtcgg ctctgagagc gttttttaca taacccttgc gggtttcccc gtcgggataa
     3241 ccgggaataa actcccttaa tggagtaaaa gcaatcaaaa cgcttaccat tataatcaaa
     3301 aagatggcaa atccccctac agcaataatc acattcatgc gcgaaagcct gtactgccaa
     3361 acctcttcaa atgtttcgtc gttaagtatg cttaaacggt acttattcct aaaattccgg
     3421 aatatcttga ttttcctttt catttgtcaa ttccattttt taatcatctg gaaaacccat
     3481 gtggcaggca tacaatcaag ctaaaagcca actcatgaat ttaatcctta tgctgaattg
     3541 aaatacccac aaagatagtg gttttataaa aaggtttgaa tggtgacgta aattgattta
     3601 aaggctaagc tgaatactga atgggtactg ctatggcata ataattttta atattttttt
     3661 ggaatgataa agtttgtgat taactttgca cccgcaaagg caagaattta gacttcattt
     3721 aaaatactat attgcggggt ggagcagttg gtagctcgtt gggctcataa cccaaaggtc
     3781 gtaggtccga gtcctacccc cgctactgaa aaccccgg
//
LOCUS       DAOSHM010000123         3777 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_22373_length_3777_cov_3.592961, whole genome shotgun sequence.
ACCESSION   DAOSHM010000123 DAOSHM010000000
VERSION     DAOSHM010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3777
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22373_length_3777_cov_3.592961"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(295..1374)
                     /locus_tag="PK839_10500"
     CDS             complement(295..1374)
                     /locus_tag="PK839_10500"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HOG73205.1"
                     /translation="MKAARLHELGKPLVVEEVSIPELNDDDVLVEVKATFVAPSMKDI
                     SHLGGNFIRPTLPAILGSDAVGVISKLGRRVKRLRVGQYVWVNSMLYYNTDEFALRGQ
                     EGLSDSMAFQGMFTFNPNNIHLMDEYQGGFAQFIKAPSVSIAALPDNFPLEYAVRLGY
                     MGTAYHALKRAGVHYGSTVLINGATGTVGTSAVLLSLAMGASKVVAVANKKDRLQRIK
                     QLNPAIIEVLSLQDGDVAGKIKELTNGKGVESFIDCLSYVDTTSTQQCLFAVKKGGTA
                     VCLGGATGSLTVPYGFLLGTEINLTGSIWFHTYEIDEIVSLASSGRLNLNSLETKKFP
                     LDMVNEAIELAGSRLGGLTTVMINI"
     gene            complement(1452..2045)
                     /locus_tag="PK839_10505"
     CDS             complement(1452..2045)
                     /locus_tag="PK839_10505"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HOG73206.1"
                     /translation="MKKLFLLLGLASALTFTVGAQTSAWVIDNMHSNFGFKISHMGIS
                     LVEGEFNEFSGTITTSREDFSDAKAEILVKAASINTDVQPRDQHLRSADFFEVEKYPD
                     ITFVSTKMERTYGNNFKLTGNLTMHGITKTIVLDVTYNGQAYEEQMKVQKAGFTVQGV
                     LNRDDFGISAFSFPLPSGAPMVGREVFLTIPLELIKQ"
     gene            complement(2100..2864)
                     /locus_tag="PK839_10510"
     CDS             complement(2100..2864)
                     /locus_tag="PK839_10510"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOG73207.1"
                     /translation="MSKLLENKTVLVTGGGSGIGRAAALGLAREGARVVVVDVNENGG
                     NETVRLIENAEGKAVFIKANISEEVEVMNLISKVVDLYGHLDGAFNNAAISGKLGKTT
                     IDYDSSDWDALMSVNLKGTWLCMKHEIIQMLKQGYGAIVNTSSDAGLIALPGLNPAYV
                     AAKHGIVGLTKIAAIEFADRNIRVNAIAPGMTHTGMTHDLIAGAPSEREVASYLPIKR
                     VADPAEIAEAAIWLLSGKASFVTGAILSVDGGSVAD"
     gene            complement(2947..>3777)
                     /locus_tag="PK839_10515"
     CDS             complement(2947..>3777)
                     /locus_tag="PK839_10515"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOG73208.1"
                     /translation="GFEEDFSNDKLFSKSGKWKKVVVVGGGPSGMKAAEIAAKKGHKV
                     VLFEKSSELGGRVKLESALPHKEELAGTSRYLKYALKKLNVDVRLNKIATKEDILAEN
                     PDITVVATGANLVNGEGTYSVEDVIGNKVKGENVLVMDFDANIEGTGAVEHLANQGKT
                     VHWVTPVFFNGQDNEVSTVLLPIYRRLGEKKVILHPMSVMINFDNGKATLLNPFFNTT
                     ETLEGIDAIVMAGTKAANNSLYNEIRSSLQNVYVIGDAAAPRNTGAAIADAMSLSVNF
                     "
BASE COUNT         1088 a          885 c          726 g         1078 t
ORIGIN      
        1 cagagaggta ggtataacat cgcatcaggt tttggcaaaa ttacaagaat tttggaagta
       61 gccaaaatta aattataaac atgatccttt agatgactaa aacatgctac atttctattt
      121 aacaactatc tacatacggc acctaaattc aaccaataaa tgctactttt tagaggacag
      181 taaaataata tttttcagga taaaggagaa aaaaattctt tttccccctt gtcctgatgg
      241 agtgtatttt ctactttacc ctgtccagaa acagggggcg aaagaataaa gcagctaaat
      301 gttaatcatg acagtggtta aaccacccag cctagatcca gccagctcaa tagcctcgtt
      361 aaccatatcg aggggaaact ttttggtttc taaagagtta aggtttagcc taccagagga
      421 tgccaaactt actatttcgt caatttcata ggtatgaaac cagattgaac cggtaaggtt
      481 aatttcggtt cctagtaaga aaccataggg aacggtgagg ctgcctgttg ctccacccaa
      541 gcaaactgct gtaccgccct ttttgactgc aaacagacat tgctgtgtag atgtagtatc
      601 aacgtatgaa aggcaatcaa taaaggattc tacacctttc ccattagtta actccttgat
      661 ctttcctgct acatcaccat cctgaagcga tagaacctca ataattgccg gatttagctg
      721 tttaatccgc tgaagtctat ccttcttatt ggcaacggca acaactttgg aagcgcccat
      781 agccaacgac agtaatactg cacttgttcc aacagttcca gtagccccat tgatgagtac
      841 tgtagagcca taatgaaccc cggctctttt aagagcatgg tacgctgtac ccatatagcc
      901 aagacgtacg gcatattcca gaggaaaatt atcaggtaga gcagcaatac ttaccgatgg
      961 agccttaata aattgtgcaa agcctccctg gtattcatcc atgagatgaa tattgttggg
     1021 gttaaaggtg aacattccct gaaatgccat ggaatcgctc aacccttcct gtccccttaa
     1081 agcaaactca tcggtattgt agtatagcat tgaattcacc caaacatact gtccaactct
     1141 cagcctcttt acccttctac caagctttga gataacaccc acagcatccg atccgagaat
     1201 agcagggagg gttggacgaa tgaaatttcc tcctaggtgt gaaatatcct tcatagaagg
     1261 agcaacaaag gtcgccttaa cttcaaccag aacgtcatcg tcattaagtt caggaatgga
     1321 cacttcttca accacaaggg gtttgcccag ctcatgcaaa cgtgcagctt tcatagcata
     1381 atttatagaa attcatatta agtgataaga atagtgaatg atataagggc aatcattaaa
     1441 gaattataac attactgttt gattagctca agtggaatag tgagaaatac ttcacgccca
     1501 accattgggg cgccagaagg taatggaaag ctaaatgctg aaatcccaaa atcgtcacgg
     1561 tttaacactc cttgaaccgt aaatccggct ttttgtacct tcatttgttc ctcataagcc
     1621 tgaccattgt atgtaacatc gagaacaatg gttttggtaa tcccgtgcat ggttagatta
     1681 ccagtgagct tgaaattatt accataggtg cgttccatct ttgtactcac aaacgtgata
     1741 tcgggatatt tttcaacttc gaagaaatct gctgacctta ggtgctggtc tctaggctga
     1801 acatcagtat tgatgcttgc agctttaacc agtatttcag cttttgcatc tgaaaaatcc
     1861 tcccttgagg tagtgatagt tcccgaaaat tcgttgaact cgccctcaac cagtgatata
     1921 cccatatggg atattttaaa gccaaaattg gagtgcatgt tatctattac ccatgcagaa
     1981 gtctgtgccc caacagtaaa agttagggct gaagccaaac caagtagcaa aaataatttt
     2041 ttcatagcaa atagttttaa ggttaatgat cagtaaggtt aaaattacat aagcgccatc
     2101 tagtcggcca ctgagccgcc atcaactgat agtatagctc ctgtgacaaa cgaggctttg
     2161 ccggaaagca accaaattgc ggcctcggca atttcggcag ggtcggcaac ccttttaatc
     2221 gggaggtatg aagctacttc cctttcgctg ggggctccgg caattaagtc atgagtcatt
     2281 ccggtatgtg tcatccctgg tgcaatggcg ttaactcgaa tattcctatc ggcgaactca
     2341 atggcggcaa tttttgttag cccaacaatg ccgtgcttag ctgcaacgta agcggggttg
     2401 agacccggta gggcaattaa tcccgcatcg gatgaggtat tcacaatagc accgtagccc
     2461 tgtttgagca tttgtatgat ctcatgtttc atacaaagcc atgttccttt aaggttaacg
     2521 ctcatcaggg catcccagtc ggacgagtca tagtcaattg tagttttccc caatttccct
     2581 gaaattgccg cattgttaaa agcaccatca aggtgtccgt acagatcaac caccttactg
     2641 atgaggttca tcacctcgac ctcttccgat atattggcct tgataaaaac ggccttgcct
     2701 tcagcattct ctatcaagcg aacggtttca tttccaccat tttcattaac atccacaaca
     2761 accacccttg caccttctct ggccaatccc aaagcagcag ctctacctat ccctgaaccc
     2821 ccgccggtta ccagcacggt tttgttttca agtagtttgc tcattatttt gtttttttgt
     2881 tttagtcaga acgaaaaatg ttaagggaaa cccctctaac ggaaaggggt ttcctatgat
     2941 tagaatttaa aaattaacgc ttaagctcat ggcatcggct attgccgccc ctgtgtttcg
     3001 cggagcagca gcatcgccga taacatatac attctgtagg ctgcttcgta tttcgttgta
     3061 aagcgaatta ttagctgcct ttgttccggc cattacaatg gcatcaatac cctcaagcgt
     3121 ttctgttgtg ttaaaaaatg ggttcaagag ggttgcctta ccgttatcaa aatttatcat
     3181 taccgacatg gggtgtagaa tcaccttttt ttcacccagc ctacgataaa ttggaagcag
     3241 gaccgtagaa acctcgttgt cctgtccgtt gaagaataca ggtgttaccc aatgaactgt
     3301 tttaccctgg ttggccaggt gttcaacagc acctgtaccc tcgatattgg catcaaaatc
     3361 cataacaaga acgttttcac cctttacctt gttgccaatg acatcctcca cagaataggt
     3421 tccctctcca tttaccaaat tagctccggt agcaacaaca gtaatatctg gattttcagc
     3481 aagtatatcc tctttggtag caattttgtt gagccttaca tcaacgttca acttcttcaa
     3541 cgcatattta agatatcgcg aagtaccagc caactcctct ttatgaggaa gggcgctttc
     3601 caactttacc ctaccgccca gctcgctgct tttctcgaaa aggacaacct tgtggccttt
     3661 ttttgctgca atttcagcgg ctttcattcc ggaaggcccc ccgcccacaa caacaacctt
     3721 tttccattta cctgatttac taaagagctt atcattagaa aaatcttcct caaaacc
//
LOCUS       DAOSHM010000124         3714 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_22905_length_3714_cov_20.870183, whole genome shotgun
            sequence.
ACCESSION   DAOSHM010000124 DAOSHM010000000
VERSION     DAOSHM010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3714
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22905_length_3714_cov_20.870183"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            51..3587
                     /locus_tag="PK839_10520"
     CDS             51..3587
                     /locus_tag="PK839_10520"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOG73209.1"
                     /translation="MRKRIALIAVYTLLFSAAGRGQYFLFDNEPPKIDLTFARHSLEA
                     EPGKTIFNVVKVANRSSQTQTFTLQLTAPAGWQIIGQDKFELTIPPLDSTLVPVRVAI
                     GQKVRGDIGYSVIANVTDSRGSSIKTEYSFIKIPREVDLRVKVLTRYEYFDQQTGHAP
                     IRVMVDNRGNREETVTINLEADSYLGIADARLQNYVQDVSIPPYTDSTLVFNVYLDRT
                     LETSREVFRVNIRAQTLDTTFRGTSWLRNLSSSYSSYIPESRKMLIAEVFGKGLFSNY
                     AKPTYSAIVQGNILLPKKREVNFFYNNYSTTPVENLYRYNRMWVGYKDGKLNVQLGDI
                     NKSMESSLYGRGGSVDVQLNRFKVEAVATQRVLNPLNNFGLKLDYSPSQSLGFEAGGT
                     YSSALNENFDSRLGLAGVRFTLAKKYRFSLQMAYNQLEFGRTPDTLQSKTALGATANF
                     TYSASQLRVSVRYKAGEKYFYSGYNGRSELMAQADYNFKNSSRLIYNAIDNRNSPGDY
                     RTYGLTYQPYTNFHRQLLTYGVNFTPRFYLFTGPGFELNSTDIFPVIRRGEDYFSTIV
                     YNYNAGLRFKGSRPGSSLTTQMFAGWVNVAHQPTHFQDPGFSIPDSKNKFDYQFFSIN
                     LRQPMWGAQAMYTNGPRSIYQQFSWYYSSRPTRLLRVMPYFDAFIYKDMLKLMVNVSY
                     TNDLVSKSSYSNITSQLYWYLPRDWQIRLLNVYTIQSRTTGTETVERFQNMYFEASVR
                     KEFGFQQPTVKYYDLKLVFFKDYNGNRIKNDNEPGIKNVLLSIQRTDTPEFPDFSSGE
                     LLSNQYGEVFFENIPAGIYDLSYNPVGTEAGTFSKADIESTIRFDKNTTLYIPFVEKN
                     KVFGKIILNRSKLSGLGKVDVSNVRITVTDSRGNTFSTLTDKNGEFMIFAPITDEYIV
                     SINNIFYEDFNLRQNNFRVQFNGYKQFEVNFVFDEKVRRINFSPSTQNLAEQGVLQVR
                     RTNLRGTIKDESSLKPLRARVNLVNTLNNSIVASIYSNPQTGDFNLSFLAGDNYLLEI
                     LADDYWYYSETLNLNQVTTFMNVSRDILLKPIAIGSKIELNIRFEANRADLGPESVAE
                     LNRLLRILRDNPNIKVEIQGHTDDLEAISNSQVGEERAKAVARYLVENGFSNLQVRGF
                     GNTVPVAPNDTEENRSLNRRVEVEVVERTTGQ"
BASE COUNT          963 a          981 c          915 g          855 t
ORIGIN      
        1 ctactttgtt gacctgattt tcaccctggt ggaaatccct taaacgattg atgcgaaaac
       61 gaattgccct catagccgtt tacacccttc ttttttccgc agctgggcgg gggcagtact
      121 ttttgttcga caacgagcca cccaaaattg accttacctt tgcccggcat agcctggaag
      181 cagaaccggg taaaacaata ttcaacgttg ttaaggtggc caaccgctcg tcgcagacgc
      241 aaacattcac ccttcagctg accgcacctg ccggttggca gataattggg caggacaagt
      301 ttgagctgac aattcctccg ctcgactcca cgctggttcc ggtgagggta gccattggtc
      361 aaaaggtgcg tggcgacatt ggttactccg taattgccaa cgttaccgat agccgcggca
      421 gctccattaa aaccgagtac agcttcataa agataccccg cgaggttgac ctgagggtga
      481 aggtgctcac ccgctacgag tacttcgacc agcagaccgg ccatgcccca atcagggtga
      541 tggttgacaa caggggcaac cgtgaggaaa cggtaaccat caacctggag gctgatagct
      601 acctgggcat tgccgatgcc cggctgcaga actacgttca ggatgtcagc attccgccct
      661 acaccgattc aacactggtg tttaacgttt acctcgaccg cacattggaa accagccggg
      721 aggttttcag ggttaacatc cgggcgcaga ccctcgatac cacgttcagg ggaacatcat
      781 ggctgcgcaa cctttcaagc agctactcca gctacattcc cgaatcgcgt aaaatgctca
      841 tcgccgaggt gtttgggaag ggactttttt ccaactacgc taagcccacc tactcggcca
      901 tagttcaggg taacatcctt ctgccgaaaa agcgggaggt taactttttc tacaacaact
      961 acagcacaac cccggtggag aacctctacc gctacaaccg tatgtgggtg ggttacaaag
     1021 atggaaagtt gaatgtgcag ctgggcgaca ttaacaagag catggagagc agcctctacg
     1081 ggcggggggg aagtgtagat gttcagctta acaggtttaa ggttgaggcg gttgccaccc
     1141 agcgggtgct aaacccgctg aacaactttg ggctgaagct ggactactct cccagccaga
     1201 gccttgggtt tgaggcgggc ggaacctaca gctccgcgct gaacgagaat ttcgactcca
     1261 ggctggggct ggcaggcgta agatttaccc ttgcaaaaaa gtaccggttt tcgctgcaaa
     1321 tggcctacaa ccagctggaa tttggaagaa cacccgatac ccttcagtcc aaaacagcgc
     1381 tgggggcaac tgcaaacttt acctactccg cctcgcagct gagggtgtcg gtgcgctaca
     1441 aggctggtga gaaatatttt tactcgggct acaatggtcg gtcggagctt atggcccagg
     1501 ccgattacaa ctttaaaaat agcagccgac ttatttacaa tgcaatagat aaccggaata
     1561 gccccggaga ctaccgcacc tatgggctaa cctaccagcc ctacaccaac tttcacaggc
     1621 agctgcttac ttatggggtt aactttactc cccgttttta cctgttcaca gggccgggtt
     1681 ttgagctcaa ctctaccgat atcttcccgg ttatcaggcg tggcgaggac tacttctcca
     1741 caattgtgta caactacaat gccggcttga ggttcaaggg atcccggccg ggcagctcgt
     1801 taaccaccca gatgtttgcc ggatgggtta atgttgcgca ccaacctacc cattttcagg
     1861 atccgggctt ttcaattccc gatagcaaga acaagttcga ctaccagttt ttctccatca
     1921 acctgcgcca gcccatgtgg ggtgcccagg ccatgtacac caacgggcca cgctccatct
     1981 accagcagtt tagctggtac tacagctccc gaccaacgcg tttgctccgg gtgatgccct
     2041 actttgacgc ctttatctat aaggacatgc tgaagctgat ggtgaacgtt tcctacacca
     2101 acgacctggt ttcaaaatcg agctactcca acatcacctc gcagctctac tggtaccttc
     2161 cgcgcgactg gcaaatcagg ctgctgaatg tttacaccat tcagtcgcgc accacgggaa
     2221 ccgaaacggt ggagcggttt caaaacatgt acttcgaggc aagcgtgcgc aaggagtttg
     2281 gtttccagca gcccaccgtt aagtactacg acctgaagct ggttttcttt aaggactaca
     2341 acggcaaccg gataaagaac gacaacgaac cggggataaa gaacgtgctg ctaagcatac
     2401 agcgtaccga taccccggag ttccccgatt tttcatccgg cgagctacta tccaaccagt
     2461 acggcgaggt gtttttcgag aatattcctg ccggaattta cgatctttca tataatcctg
     2521 tgggtaccga ggccggaaca ttttccaagg ccgatattga gtcaaccatt aggttcgata
     2581 agaatactac cctatacata ccctttgttg agaagaataa ggtttttggg aaaatcattc
     2641 tcaatcgcag caagctatca ggtcttggaa aggttgatgt ttcgaatgtg aggattactg
     2701 taaccgatag tcggggtaac accttctcaa cgttgactga caagaacggg gagttcatga
     2761 tttttgcccc aatcaccgat gagtacatcg ttagcatcaa caacatattc tatgaggatt
     2821 ttaacctgcg ccagaacaac ttcagggtgc agtttaacgg ctacaagcag tttgaggtga
     2881 actttgtatt cgacgagaag gttcgtagaa ttaacttcag cccatcaaca caaaacctgg
     2941 ccgagcaggg agtgcttcag gttcggagaa ccaacctcag aggaaccata aaggatgaat
     3001 ccagccttaa accccttagg gcacgagtta acctggttaa cacccttaac aactcaattg
     3061 tggcctcaat atactccaac cctcaaaccg gcgactttaa cctgtcgttc ctggctggag
     3121 ataactacct gctcgagatt ctcgccgacg actactggta ttactctgag acgcttaacc
     3181 ttaaccaggt taccaccttt atgaatgtta gccgcgacat actgctaaaa ccaattgcca
     3241 taggttcaaa gatagagctt aacatccggt ttgaagccaa cagggctgat cttggccccg
     3301 agtccgtggc tgagttaaac cggctgttga gaatactccg cgataacccg aacatcaagg
     3361 ttgagattca aggacatact gatgacctgg aggccattag caactcacag gtaggcgaag
     3421 agcgcgccaa ggcggttgcc cggtacctcg ttgagaatgg tttcagcaac ctgcaggttc
     3481 gggggttcgg caatacagta cccgtggctc ccaacgatac ggaagagaac aggtcgctta
     3541 accgaagggt tgaggttgag gtagtggaga gaaccaccgg gcagtaacca agcacttttt
     3601 cccggacaac caagaatatt cgttagctag attgggatac tagacggagc ccgccaagaa
     3661 ctcgttgatg gtaaatccgt cagctgacgg acaagttccg ccagtaggct gaca
//
LOCUS       DAOSHM010000125         3612 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_23813_length_3612_cov_8.078999, whole genome shotgun sequence.
ACCESSION   DAOSHM010000125 DAOSHM010000000
VERSION     DAOSHM010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3612
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23813_length_3612_cov_8.078999"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(200..1117)
                     /locus_tag="PK839_10525"
     CDS             complement(200..1117)
                     /locus_tag="PK839_10525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG73210.1"
                     /translation="MEPAKTILHIGYPKTATKWFQKKFYPKLNNIFFVKREEVFKWLA
                     FRNIFDYSPVNTLEHLAGIANRKRLVICDEIILGGLDIGFGSGEYVYMMANRLRETFC
                     DASIVIFIRNQHDALESAYSHYIKAGGTYSVKNYLGISKKFTRPFQNYHFFNSDLFQY
                     SSIIGLYSKLFGRENVHVYLYEDFQSNIEVFINQFCTDFDLLPDGELNFQPTNVRLPF
                     FSIRLMRLFNHFTAKNTIFKHYFFSLPRFYRILQSGVRRLAGILPFNRPFTFSSDIHS
                     WIDERFRMSNRQLSDWVDMDKLKDYGYPL"
     gene            complement(1121..2014)
                     /locus_tag="PK839_10530"
     CDS             complement(1121..2014)
                     /locus_tag="PK839_10530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG73211.1"
                     /translation="MPVIVHIGYPKTATTWFQQRFFPYIENYSYISYSRVNRIFLYSD
                     FFEFNSQKAREELLAESKNRNLLLSSEFLTTAINFGWQHGYYAAGVAQKIHKTFPEAK
                     IVIFIRRQQSLICSAYQQYLKNGGTYSFKRWLYSGDVFCPEHLYYDGLISYYDKLFGR
                     EMVKVYFYEDFKEDNIGFLTRLNREFSFHVDMNAISLKPENVGLRRRVVPFLFMVNHF
                     YKKPVGRKRYLVHVPGMTSVGKAIFKFLNPLPIFGRFLSEDKMLKPEDERKLYDFYSE
                     SNRRLANRVDIKKLQHHGYYL"
     gene            2391..>3612
                     /locus_tag="PK839_10535"
     CDS             2391..>3612
                     /locus_tag="PK839_10535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG73212.1"
                     /translation="MRKFTTMVLVLLAQISFAQNWSWVKQFNQSPNPGKPNELDIYDL
                     KFSNNGNLYVTGSYISNVVIGGVKYLGYPDASSTSSDIFIAKFDENGNVLWVKTVGSP
                     SGDLPEGITVDSNDNIYLSGRFSGTCDFGSSLTLVSSGGFDSFIAKYNSSGQIQWVIK
                     SAYGSANDRTTAITLAKDGFIYVTGLSKGNFSVGDGTTQDTYTNPDSKTDLFLASYTT
                     NGAYRWSRQLPGNDDNVIFRSITCDDQNNLYAGGALAGKLTIDGIDYTSAGSGDIILL
                     KAHPTDGSAIWVRKGGSTLDDQLNSIAVNDNFAYIIGYIQGTGTIDSTATKLSSQFST
                     KGGNDIFLAKYNLEGRLLWKKTLGSAGADVGYGLNTINNILVATGYFSNTLNFNLSSI
                     TSGGGMDAAFFVFDV"
BASE COUNT         1131 a          717 c          700 g         1064 t
ORIGIN      
        1 agctggccat taaggagaag taccgctttg gaacctatgg cgatgccatg ctcattattt
       61 aagcgtaaaa ccagggagta aatactatat caaacctgca gccatccccc gaacgcaaag
      121 aaaagagggt ggctgttttt ttatttactc caaacatcaa tttacatgga tattaggctg
      181 taacctgctg aaaaaatgcc tacagcggat atccataatc cttcagctta tccatatcca
      241 cccagtcgga aagttgtcgg ttgctcatcc tgaaccgctc atcaatccag ctgtgaatgt
      301 cgctgctgaa ggtaaaagga cgattaaatg gtaaaattcc agctaaacgc ctgacccctg
      361 attgtaatat tctataaaag cggggtaatg agaagaagta gtgtttaaat atggtatttt
      421 ttgcagtaaa atggttaaat agcctcataa gcctgattga aaaaaacgga agtctcacat
      481 tagtaggctg aaaatttaac tcaccgtcag gaagtaggtc aaaatcggtg cagaactggt
      541 ttataaaaac ttcgatgttc gactgaaagt cctcgtaaag gtaaacatga acattttcac
      601 gcccaaagag cttggagtat aaacctatga tactactgta ctgaaataaa tcggaattga
      661 agaaatgata gttttgaaat ggccttgtaa actttttgct tatcccaagg taatttttta
      721 ccgaatatgt acctccagct ttaatataat ggctatatgc cgattcaagg gcatcgtgct
      781 ggttgcgaat gaaaattacg atggaggcat cgcaaaaggt ttctctaagc cgattggcca
      841 tcatataaac atattcgccc gatccgaaac caatatccag tcccccaagg ataatctcat
      901 cacaaattac caggcgtttt ctgttagcta tgccagctaa atgctccagg gtattgaccg
      961 gagagtagtc aaaaatattc ctaaaagcca accatttgaa aacctcctcc cgttttacaa
     1021 aaaaaatatt atttaacttg gggtaaaatt tcttttgaaa ccacttggta gctgttttgg
     1081 gatatcctat gtgaagtatt gtttttgccg gttccattgc ctaaaggtaa taaccgtgat
     1141 gttgcaactt tttaatatcc acccgatttg ccaatcttct attcgactcg ctgtaaaaat
     1201 cgtatagttt cctttcatcc tcaggcttca gcattttgtc ctctgaaagg aaccttccaa
     1261 aaataggcaa ggggtttaaa aacttaaaaa tagctttccc taccgaagtc ataccgggca
     1321 catgaactaa gtaacgtttt cgccctactg gttttttata gaaatgattt accataaaaa
     1381 gaaatggaac tactcttcgt cgcaggccta cattttcagg ttttagagaa atggcattca
     1441 tatcaacatg aaagctgaac tctctgttca accgggttaa aaagccaata ttatcttcct
     1501 taaaatcttc ataaaaataa accttaacca tctccctccc aaaaagctta tcgtagtaac
     1561 ttatcaaccc gtcgtagtag agatgttcag gacagaaaac atcgccggaa taaagccatc
     1621 gcttaaaact gtaggttcct ccattcttta agtactgctg ataggcagaa caaatcaacg
     1681 attgctgacg tcgaataaat atgacaattt tggcttcggg aaaggtttta tgaatttttt
     1741 gagccacacc tgctgcatag taaccatgtt gccacccaaa gttgattgcc gttgttaaaa
     1801 actctgaact taaaagtaaa tttctatttt tactttcagc aagtaattct tctctggctt
     1861 tttgggaatt aaattcaaaa aaatcagaat acagaaagat cctattaacc ctactatagg
     1921 aaatgtagct atagttttca atgtatggga agaagcgctg ctgaaaccaa gttgttgcag
     1981 ttttgggata tccaatgtga actataacag gcatgatttt gaccagcata taatttgaat
     2041 aggtaaaaat acctcatttc tcatatcttt taaataaatt gcccgtaaat tagtagcaaa
     2101 tagtacaaaa ccgtacaaat ttcaaaataa aaacatctat ttgccaatac tttaatattg
     2161 tactacaagt aaataagtat atttggtgta atttttgttg attcatttaa catgttgaat
     2221 catagcgatt tggtgtgttg tgcaataata tagaatcata accctgttta tgctttttgg
     2281 gaggtaggta gttgaaaatt aggatatata cacaaaaacc cagttgtttt cgacccactg
     2341 cgtaccaagt taatacgttt atgtttttta aaacatttaa acactaagac atgagaaaat
     2401 ttacaacaat ggttctggtt ttgttagctc agatatcttt cgctcaaaac tggagctggg
     2461 tgaagcagtt caatcaatca cctaatccag gcaaacctaa cgaactggat atttacgatt
     2521 taaagttcag caataacggt aacctctatg taaccggttc gtacatatca aacgtggtta
     2581 ttggtggcgt aaagtacctg ggttatcctg atgcttcatc tacatcatcg gatattttta
     2641 ttgcaaaatt cgatgaaaat ggaaatgttc tatgggttaa aacggtaggt agcccttcgg
     2701 gtgatttgcc cgaagggatt accgttgata gtaacgacaa catttacctc tccggaagat
     2761 ttagcggaac atgtgatttt ggctctagtc taactttggt ttccagcgga gggtttgata
     2821 gttttattgc gaaatataac tcatcggggc aaattcaatg ggtaattaaa tcggcttatg
     2881 ggagtgcaaa cgatcgcaca actgccatta cgctggcaaa agatggtttt atctatgtaa
     2941 cgggcttatc taaaggaaac tttagtgttg gcgacggaac aacccaggat acctacacca
     3001 atcccgattc aaaaaccgac ctttttctgg ccagctatac taccaatggt gcataccgct
     3061 ggtccaggca actccccgga aacgatgata acgtcatttt tagatcaatt acctgcgatg
     3121 atcagaataa cctgtacgca ggaggagcgc ttgccggtaa acttaccatt gacgggattg
     3181 attacacctc tgcaggtagt ggcgatatta tcctgttgaa agcacaccca accgatggtt
     3241 ctgctatatg ggttcgtaaa ggtggcagca cattagacga ccagctgaac agtattgccg
     3301 ttaacgataa cttcgcctac atcattggct atatccaagg aacgggaacc atcgacagca
     3361 ccgctacaaa gctatcctct cagttttcaa ccaagggtgg taatgatatt ttcctggcta
     3421 agtataacct tgagggacga cttctttgga agaaaacatt gggtagcgcc ggagcagacg
     3481 tgggttacgg gctgaacact attaataata ttctggtagc aactggctac ttttcaaaca
     3541 ccctaaactt taacctgagc tccatcacct ccggaggtgg catggatgca gctttctttg
     3601 tatttgacgt gg
//
LOCUS       DAOSHM010000126         3518 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_24740_length_3518_cov_22.004909, whole genome shotgun
            sequence.
ACCESSION   DAOSHM010000126 DAOSHM010000000
VERSION     DAOSHM010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3518
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24740_length_3518_cov_22.004909"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3441
                     /locus_tag="PK839_10540"
     CDS             <1..3441
                     /locus_tag="PK839_10540"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOG73213.1"
                     /translation="MENQTNPEEFYRLLAISKERMKELAAINNLVSIIKENKPIPETL
                     YQLCLVLADAWQYPEYTAVRIRYGQYEFQTRNFRETPWSQKEEFESIDGIRGLLEVYY
                     TQKFPEADEGPFLKEERDLIKNLSNIIVGYINSVKGQEVLKDKAKPKRRGFEVEPQSK
                     SLLQRFINQQNVDRDIYHDLMPFKVHEILLISTLYDAYSIEKEDRLTDNILGEYTKLS
                     LSSVPRITGVSNLDEALDKLEERYFNMIIIMMGADTVTPLKMAQRIKGEYNHIPIYLL
                     VNNSVIVDEMEKNPVAITGIDRMFVWNGEPKIFFSMIKLLEDRVNIENDTRIALIRLI
                     LLVEDSPKYYSRYLPLLYGSVLDQTKRMIEDVSTDELYKVLRIRIRPKILLAENYEEA
                     MELFSRYKDFMLCVISDVKFFRKGKLDEHAGVSLVEQVRKELPNLPIILQSFDRSNEE
                     TALKLKVAFLDKNSESLMLDIKNFLSNYLGFGDFVFKDSEGNPITIASTMEEFEKALR
                     IIPDESLLYHAQKNHFSMWLAARGEIQVARIIHPSRIDDFSGPDEIREYLILSLRKYR
                     QEKRRGKIVTFDSDWMVDESNIVSLADGSFGGKGRGLSFINTLLYTFDVSQYTPNINL
                     HTPRTSIIGTNEFENFMANNSLYEVVFSAKSYDEIKRYFLKAELSNQLKIKLDRLLQI
                     YYRPLAIRSSGMLEDSIMQPFAGIFDTYMIPNAHADRSIRLQSLMNAIKLVYASVFSP
                     TARAYVKAINFKIEDEKMAVIVQEVVGNKFENYYYPHISGVAQSYNYYPFGHINPEDG
                     FANIAVGLGKHVVEGGKSYRFCPRYPTLINYTLEDLVKNSQTDFLAIDLSRPDFDLLS
                     GDEAALARLDMSYAEQHGNLKHCASVYSSNNNSLIAGIGQPGPRVINFPNILKYNYIP
                     LSQTIDVILDIVKEAFGSPCEIEFAVDLNKDANFKASFFLLQIKPLIGNYTEYKVNLD
                     ELDLNSVILLSNTGMGNGSISNLYDVVYIKRNVFDKSMTPTMALEVEAINEKLATEGK
                     NFILIGPGRWGTRDRWIGIPVTWPQISNAKVIVETSLDDFPLDASSGSHFFHNVISMN
                     VGYCSVPNNDSFARIAWSKLDELPAYNETKYFRHVRFPKPLQVKMDGTQRLIVVTHEP
                     "
BASE COUNT          983 a          775 c          828 g          932 t
ORIGIN      
        1 atggaaaatc aaactaatcc cgaggaattc taccgcctgc tcgccatatc gaaagagcgg
       61 atgaaggagt tggctgcaat taacaacttg gtttccataa taaaggagaa caagccaatt
      121 cctgaaacgc tttaccagct ttgtcttgtt ttagcagatg cttggcagta ccccgagtac
      181 acagcagtaa ggattaggta tgggcagtat gagtttcaaa cccgtaattt tagggaaacg
      241 ccttggagcc aaaaggagga gtttgaatct attgatggaa taaggggttt actggaggtt
      301 tactacaccc aaaaatttcc ggaagctgat gaaggccctt ttctgaagga agagcgcgac
      361 ctgattaaaa acctatccaa cataatagtt ggctatatta acagcgtaaa gggtcaggaa
      421 gttcttaaag ataaggctaa gccaaaacgt agaggttttg aggttgagcc ccaaagcaaa
      481 agcctgcttc agcgatttat caaccagcag aatgtagata gggatatcta ccacgatttg
      541 atgcccttta aggtgcatga gattttgctt atttccacgc tgtacgacgc ctacagcatt
      601 gagaaggagg accggctgac ggacaatatt ctaggagagt acacaaagtt gagcctttcc
      661 agcgtgcccc gaattacagg ggtttccaat cttgatgagg ctcttgataa gctggaggag
      721 cgctacttta acatgattat catcatgatg ggcgccgata cggttacccc cctaaagatg
      781 gcccagcgca ttaagggcga gtacaaccat attccaattt acctgctggt aaataatagc
      841 gtgattgttg atgagatgga gaaaaatcct gttgccatta ccggcatcga caggatgttt
      901 gtgtggaacg gggaaccaaa gatttttttc tccatgatca agctactcga ggatagggtc
      961 aatattgaga atgatacccg tattgcgctc ataaggttaa tcctgttggt tgaggactcc
     1021 ccaaaatact actcccgata cctgcctcta ctctatggta gtgtacttga ccaaaccaaa
     1081 aggatgatcg aggacgttag caccgacgag ctttacaagg tattacggat taggattcga
     1141 ccaaaaatac tcctggccga gaactacgag gaggccatgg agctgttctc acgttacaag
     1201 gatttcatgc tttgcgtgat ttccgatgtc aagttcttta ggaaggggaa gctggacgag
     1261 catgctgggg tgagcctggt tgagcaggtg cgtaaggagt tgcccaattt gcccattatt
     1321 ctgcagtcgt tcgacaggtc caatgaggaa accgcgctta agctcaaggt ggcatttctg
     1381 gacaagaatt cggagagcct gatgctcgac atcaagaatt tcctgagcaa ctacctggga
     1441 tttggcgatt ttgtgtttaa ggatagcgag ggcaacccca ttaccattgc ctccaccatg
     1501 gaggagtttg agaaggctct gaggataata cctgacgaga gccttttgta tcatgcccag
     1561 aagaatcact tttccatgtg gctggccgcc aggggtgaaa ttcaagttgc acgaatcata
     1621 catccttcaa gaatagacga tttctcgggt cccgatgaaa ttcgcgagta ccttattttg
     1681 tccctcagga agtacaggca ggaaaagcgt agagggaaga tagttacctt tgatagcgat
     1741 tggatggttg atgaaagcaa cattgttagc ctggccgatg gttctttcgg gggaaagggg
     1801 aggggattgt cgtttatcaa caccctgctc tacacgtttg atgtttcgca gtacacccca
     1861 aacatcaacc tgcacacccc aagaacatcc attatcggca caaacgagtt tgagaacttc
     1921 atggctaaca atagccttta cgaggtggtt ttcagcgcta aaagctacga tgagattaaa
     1981 cggtattttc tgaaggcgga gctatcaaat caactgaaaa tcaagttgga caggttgctg
     2041 cagatttact ataggccact tgccatccga tcttcaggta tgttggaaga ctccattatg
     2101 cagccttttg cgggtatttt tgacacctac atgattccta atgcccatgc cgaccgctca
     2161 atccgacttc aaagcctgat gaacgccata aagctggttt acgcctccgt tttctcaccc
     2221 acagcccgtg cctatgttaa ggccattaac tttaagattg aagatgaaaa gatggccgtt
     2281 attgttcagg aggtggtggg aaacaaattc gaaaactact actatcccca tatcagcggg
     2341 gttgcacaat catacaacta ctaccccttt gggcacatta accccgaaga tggatttgcc
     2401 aatatagccg tggggcttgg taagcatgtg gttgaaggag gaaaatccta tcggttttgc
     2461 ccccggtacc caaccctaat taactacacc ctggaggacc tggtaaaaaa ttctcaaacc
     2521 gattttctcg ccattgacct cagcaggcca gacttcgact tgctttcagg tgacgaggct
     2581 gcccttgcgc gtctggatat gagctatgcc gagcaacatg gtaatttaaa gcattgtgca
     2641 tcggtttata gctccaacaa taatagcttg attgccggaa tcgggcaacc gggtccgagg
     2701 gttatcaatt tccctaatat tctcaagtac aactatattc ctctttccca aacaattgat
     2761 gtaatcctcg acattgtaaa ggaagccttt ggctccccct gcgaaataga gtttgctgtt
     2821 gatctgaata aggatgccaa tttcaaggct tcattcttcc tgcttcaaat taaacctctt
     2881 attggcaact acaccgagta taaggtgaac ctggatgagc tcgatttgaa tagtgtcata
     2941 ctcctttcaa atacaggaat gggtaacggc tcaattagta acctttacga tgtggtgtac
     3001 atcaaacgaa atgtttttga taagtccatg accccgacaa tggctttaga ggttgaggct
     3061 attaacgaaa agctggctac tgagggaaaa aatttcattc tcattggccc cggaaggtgg
     3121 ggaacccgcg ataggtggat tggcattccc gttacttggc cacaaatttc caatgccaaa
     3181 gtaattgtcg agactagctt ggatgatttt cccctcgatg cctcatccgg ctcccacttt
     3241 tttcacaatg ttatttccat gaacgtggga tactgctctg ttcctaacaa cgatagcttc
     3301 gcacgaattg catggagtaa gctcgatgag ctacctgctt acaacgaaac taaatatttc
     3361 aggcatgtcc gctttcctaa acctctgcag gttaaaatgg atggcacgca acgtttgatt
     3421 gtggttactc atgaaccctg agtatgctgg agcgtctagc tggaagcttc tacagttaaa
     3481 gaaaaaaata attccagatt caggaacttg tccgccgg
//
LOCUS       DAOSHM010000127         3215 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_28169_length_3215_cov_8.730696, whole genome shotgun sequence.
ACCESSION   DAOSHM010000127 DAOSHM010000000
VERSION     DAOSHM010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3215
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28169_length_3215_cov_8.730696"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..116
                     /locus_tag="PK839_10545"
     CDS             <1..116
                     /locus_tag="PK839_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005645361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HOG73214.1"
                     /translation="KCEVNPSDTPDTLAQKVHVLEYQHYPRVIESLLTSLI"
     gene            complement(257..469)
                     /locus_tag="PK839_10550"
     CDS             complement(257..469)
                     /locus_tag="PK839_10550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG73215.1"
                     /translation="MRMLSVAYLVATSCCTVLYIPNSYNSPLLRGKEDARANFPAGTP
                     GFMSKMGYKRVRFYTCIGVSLPFTHL"
     gene            781..1305
                     /locus_tag="PK839_10555"
     CDS             781..1305
                     /locus_tag="PK839_10555"
                     /inference="COORDINATES: protein motif:HMM:NF024954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HOG73216.1"
                     /translation="MGRKSLQVQGYSSEEIRALFTKDERYTIGIRLYAIYQVSLGQAS
                     RKLESLYNTSFKQITNWVHRFEEFGLEGLKDKPKSGRKPRLAEEQLNELSSVLQNNRP
                     DEFGYNTATWNGPILKDYIEKHYHVAYQKAQVYNLLKKLGFTYQKGRAQYPEADEGKR
                     EEFKTTLKKTKRGT"
     gene            1316..1828
                     /locus_tag="PK839_10560"
     CDS             1316..1828
                     /locus_tag="PK839_10560"
                     /inference="COORDINATES: protein motif:HMM:NF024751.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HOG73217.1"
                     /translation="MLFEDENSLRNTATISYSWAVRGKQPRVVSKQNRRERQTTFGSI
                     NIRTGQMVINFSDKGNFKSFKKHLKKILHTFKSAPKIILVLDNVRYHHAKLLKKFLEE
                     NQRLEILYLPPYSPDLNPIERAWWFMRKSITHNRFMESLKLRKAKFWQMFSHFQVPNE
                     KILTICAIFY"
     gene            complement(1948..2559)
                     /locus_tag="PK839_10565"
     CDS             complement(1948..2559)
                     /locus_tag="PK839_10565"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOG73218.1"
                     /translation="MISVQIVDDHRILVDGMLRLINESGVAQVTGTAYCGKEALDMLR
                     LKEPQVLLLDINLPDANGVDLCATIKEKYPNVKVIALTSFGEYTIVRRMLEAGANGYV
                     LKNAMAEEVLDGIECVASGDTFLCDEVDLLMKKQSSSAIWLTPRERELLKLIVEGYTN
                     PEIADKIFLGVETVNSYRKNLLFKLNARNTAVLVKMAIEQKLI"
     gene            complement(2571..>3215)
                     /locus_tag="PK839_10570"
     CDS             complement(2571..>3215)
                     /locus_tag="PK839_10570"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HOG73219.1"
                     /translation="QQIKQLEQEKQLVATQAILEGEATERKRLARDLHDGLGGMLSVV
                     KMGLHDVKSGVALDSDDVLRFNKVLEMLDSSIRELRRVAHNMMPESLMRYGLKASLTD
                     FCGNIPNVQFHYFGNEQRLDSKLEMMIYRTAHELVNNALKHAQAQHINIQIVQGTDRV
                     SLIVQDDGVGFDPKVKSEGTGLDNIRCRVQLFNGTMDIFSKTGNGTEVNVEFKL"
BASE COUNT          998 a          729 c          668 g          820 t
ORIGIN      
        1 ctaaatgcga ggtaaaccca tccgatactc ccgataccct tgcccaaaaa gtgcatgtgc
       61 tggaatatca gcactaccca agggtaatcg agtcgctact aaccagcctt atttaagctg
      121 aattgcctat ttggggcatg taaccggcaa caagcagccg gggtatggct tatccagcag
      181 ctatttctgg ttatgcctgc caaaattaaa gtgaagacct accgggaatg taaacgggtt
      241 gtaatcccgg tcgcccttat aagtgcgtga atggcaggga tactcctatg caggtgtaga
      301 atcgaacccg cttgtagccc attttggaca taaagcctgg agttccagcc gggaagttgg
      361 cccgggcatc ttctttgccc ctgagtaaag gggagttata gctgttggga atatacaaaa
      421 cggtacaaca ggaagtggct accaggtagg caacggacaa catcctcata aagttttggg
      481 cagcattcct ctcatcggtt tttaaaatat ttaaatgtat ggctcaaact caacgtcagg
      541 ttgccttgca caaatcattc ttctccccac ataaacgcca accataggta ggtaccgccc
      601 accggtaaat tgaatgcctc tcctaatcca ccggaaacga aatagcacaa aaaaagatca
      661 gccatttgaa agttaaaaaa cgcccacacg ccaacctgtg gatcacattc atttatataa
      721 agtataattt gtaactacac attaaatgta gtaattttga aaaaactaaa ttactacatt
      781 atgggaagaa aatcattaca agttcagggc tatagttcag aagaaattag agctctgttt
      841 actaaggatg aaagatatac catcggcatc aggctgtacg cgatttatca ggtttcactt
      901 gggcaagcca gtcgcaaatt agaaagctta tacaatacca gttttaagca aatcaccaac
      961 tgggtacatc gatttgaaga atttggatta gaggggctga aggacaaacc caaaagcggc
     1021 cgtaagccaa ggttagctga agagcagttg aatgagctga gtagtgtgtt gcaaaataat
     1081 agacccgacg agtttggcta caatacagct acatggaatg ggcctatact aaaagattac
     1141 attgaaaaac actatcatgt agcataccaa aaagcgcagg tttacaactt gctgaagaag
     1201 cttggtttta cctatcaaaa gggcagagcc cagtatcccg aagcggatga ggggaaaaga
     1261 gaggaattta aaacgacatt aaaaaaaact aaaagaggaa cctgatagca ccgttattct
     1321 atttgaggat gagaatagct taagaaacac ggcaacgata tcatacagct gggcggttcg
     1381 tggaaagcaa cccagggtag tgtcaaagca aaacagaaga gagcgccaaa caacttttgg
     1441 cagcataaac ataaggacag ggcagatggt cattaacttt tcggataaag gcaacttcaa
     1501 gagcttcaaa aaacatttga agaaaatact gcacacattc aaaagtgctc caaagattat
     1561 tttggtgctt gacaacgtaa ggtatcacca tgcgaagctt ctaaaaaagt ttttggagga
     1621 aaaccaaagg ttggaaatcc tttatttgcc cccatattca ccagatctaa atccaattga
     1681 aagggcttgg tggttcatga gaaaatcgat aacccacaat aggtttatgg aatccctgaa
     1741 gttgagaaaa gcaaaatttt ggcagatgtt ttctcatttt caagttccaa acgaaaaaat
     1801 attaactata tgtgcaattt tttattagac tttatataaa atgcgagcta atgtgtgcgt
     1861 ggtgaactaa gaacgaacaa aaaaacgaga agccccattg tagcaactac aatggggctt
     1921 ctgcttggga aaggaaatga gtaataacta tatcaacttt tgctcaatgg ccattttaac
     1981 cagcaccgca gtatttcggg catttagctt aaaaagaagg ttcttacgat agctattcac
     2041 cgtttctacg ccaagaaaaa tcttgtcggc aatttcgggg ttggtatagc cctccacaat
     2101 aagctttagc agttcgcgtt cacgaggggt taaccaaatc gcgctgctcg actgcttttt
     2161 cattaaaagg tcaacctcat cgcaaagaaa ggtatcgcca ctggccacgc actcaatacc
     2221 atctaacacc tcctcggcca ttgcgttttt tagcacataa ccgtttgcac ccgcctcaag
     2281 catgcgtcgt actatagtgt actcgccaaa actagtaagt gcaataacct taacattagg
     2341 atatttctct ttgatggttg cgcaaagatc aactccattg gcatcgggaa ggttgatatc
     2401 cagcaaaaga acttgaggct cctttaggcg aagcatgtcc agagcctcct tgccacaata
     2461 ggctgtgccg gttacctgtg caacacccga ctcgttaatc agcctaagca ttccatcaac
     2521 cagaatacga tggtcgtcca ctatctgtac gcttatcatg ccaatatccc ctataattta
     2581 aactcaacgt ttacctctgt gccattacca gtctttgaaa aaatatccat agttccgtta
     2641 aacaattgca cccggcaacg gatattgtca agccctgtgc cctcggattt tacttttggg
     2701 tcgaacccaa ccccatcgtc ctgcacgatt aacgaaaccc tatctgttcc ctgaactatt
     2761 tggatattga tatgctgcgc ctgagcatgt tttaaagcgt tattcacaag ttcatgagcg
     2821 gtgcggtaaa tcatcatctc cagcttgcta tcaagccgtt gctcgtttcc aaagtaatgg
     2881 aactgcacgt tgggtatatt gccacagaaa tcggttagcg acgcctttaa accgtagcgc
     2941 atcagcgatt caggcatcat gttatgcgct acacgccgta actcgcgaat ggagctatcc
     3001 agcatttcga gcactttatt aaagcgcaac acatcgtcgc tatcaagggc aacaccactt
     3061 tttacatcgt gcaaacccat cttaacaacg gagagcatcc ctcctagccc atcgtgcaaa
     3121 tcgcgagcca agcgcttacg ctccgttgcc tcgccttcca gaatggcctg cgtggcaacc
     3181 agctgcttct cctgctctag ctgtttaatt tgttg
//
LOCUS       DAOSHM010000128         3057 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_30251_length_3057_cov_19.593271, whole genome shotgun
            sequence.
ACCESSION   DAOSHM010000128 DAOSHM010000000
VERSION     DAOSHM010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3057
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30251_length_3057_cov_19.593271"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            366..1397
                     /locus_tag="PK839_10575"
     CDS             366..1397
                     /locus_tag="PK839_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOG73220.1"
                     /translation="MFRISSLTSALFFSLAILAACVPAKEFQALQDKNAKCEEEREKL
                     STENEDLSSRSAELQAKNILLEAKVSTLASDSAICAVELSKTREQLATLNERYADLQS
                     IQDGVISGSKDETRKLLAQIQKNQEELQQKEDALKELERKLYQRKLGLDQVQANLDKM
                     SKDLEERNARLVELERMLNSKDSLMNALRKKVADALFGFEGKGLTVSIQNGKVYVSLD
                     EKLMFKSGRYEIDAKGAAAIKQLVPVLEQNPDINIMVEGHTDDVPYRGTGDLMDNWDL
                     SVKRATTIVRLLLNGSKIDPVRVTAAGRSHFLPVDRAKTPEARQKNRRTEIILTPKMD
                     EILKLLEAN"
     gene            1507..2604
                     /gene="ychF"
                     /locus_tag="PK839_10580"
     CDS             1507..2604
                     /gene="ychF"
                     /locus_tag="PK839_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007661265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HOG73221.1"
                     /translation="MGLQCGIIGITNVGKTTIFNCMSSTKAAASSFAFSASKSNISII
                     NVPDSRLYELEKFQPTERIVHTTVEIVDIPGLAKGSNKGEGVGNKFLADIRNCDALIH
                     VLRCFDDPNLPHVDGSVDPVRDIETVNFELQVRDLESIEKKLQRLEKAAKVGDKEAKQ
                     AAAVLGVYKNHLESFQNAATAPVDEQDKKYVEDLFLLSTKPVIYVCNVDDQSAVTGNH
                     YVDKVREFLKNEQTEILVIAGKLEAEIAELENEDDRLAFLSDVGLTEPGVNKLIRAAY
                     SLLNMETFFTVGPKEIRAWTIKKGMTAPQAAGVIHSDLERGFIRAEVMKYNDFITLGS
                     EQACKEKGKLFVEGKNYVVEDGDILHIRFNV"
     gene            complement(2876..>3057)
                     /locus_tag="PK839_10585"
     CDS             complement(2876..>3057)
                     /locus_tag="PK839_10585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG73222.1"
                     /translation="RIKKFKQDSKTCPPTGGFVCVCTLPLLMASPRFAATSNTPAIRY
                     HFIPTRRVQELASCR"
BASE COUNT          848 a          673 c          782 g          754 t
ORIGIN      
        1 aaggataatg tgaataaaaa cacggcaccc tatggcttca aatccatggt ataagttaga
       61 tttcccccgc taccctgcga gccccctcgt gaggtagata agaaataatg gtaaagtgtg
      121 gaaggaatag cctattggtg ctttacctgc tgttaaagtg tatttgaaag gtcgccgaat
      181 ggtagaaccg attcgcccgc cggcggagag cttggcaaag gcaaccatgc ggcagcaaag
      241 gcgtgtattt tttaattcac acaccgaacc aaacgaagtg aattgaggtg aaaaccttaa
      301 ttttataggt ccgtttacat gtataaaatt tatgtacatt tgctttcaaa ttaacccctt
      361 accagatgtt tagaatttca tcgttaacaa gtgctttatt ttttagcttg gctatcctag
      421 ctgcatgtgt gcctgccaag gaatttcaag cgctacagga taagaatgcc aagtgtgagg
      481 aggagcgcga aaagctgagt accgaaaacg aggatttaag ctcccgatcg gccgaacttc
      541 aggcaaagaa tattttgctg gaggcaaagg taagcacgct ggcctccgat agcgcaatat
      601 gcgctgttga gctgagcaaa acaagggagc agctggcaac ccttaacgag cgctacgccg
      661 acctgcagag tattcaggat ggggtaatct ccggaagtaa ggatgaaaca agaaagctgc
      721 tggctcaaat tcagaagaac caggaggagc ttcagcagaa ggaggatgcc ctgaaggagc
      781 tcgagcgaaa gctctaccag cgcaagcttg gtctcgacca ggttcaggca aatctcgaca
      841 agatgagcaa ggaccttgag gaaagaaacg cccgtctggt cgagctcgaa cgaatgctta
      901 acagcaagga ctccctaatg aacgctctta ggaaaaaggt tgccgatgcg ctttttgggt
      961 ttgaaggcaa ggggctaact gtttccattc aaaacggcaa ggtttacgtt tcgctggatg
     1021 agaagctgat gttcaaatcg gggcgttacg agatagatgc caagggggct gctgccataa
     1081 aacagctggt gccggtgctc gagcagaacc cggatatcaa cattatggtt gaaggtcata
     1141 ccgacgatgt tccctaccgg ggaacgggcg acctgatgga taactgggat ttgagcgtta
     1201 aacgcgctac aaccattgtt aggttgctgc tcaatggaag caaaattgat cctgttcgcg
     1261 taaccgctgc cggacgtagc cattttttac ctgttgatag ggctaaaact cctgaggcca
     1321 ggcagaaaaa ccgccgaact gaaatcatcc ttacaccaaa aatggatgaa atattaaagc
     1381 tactcgaggc caactagaca gaagttgcct acactccgtt caaccctgag gttataggag
     1441 gtttttcggc ctgtttttgc ataatcgggc aattgccata aatttgtacc cattaaatca
     1501 ccgtttatgg gtttacagtg tggaattatt gggataacca atgtggggaa aaccaccatt
     1561 ttcaactgca tgtcgagcac caaggcggct gccagcagtt ttgcctttag tgccagcaag
     1621 tcgaacataa gcatcattaa tgttcccgat agccggctct acgagctgga aaagtttcag
     1681 cccaccgagc gcattgttca cactacagtt gagattgttg atatacccgg gttagccaag
     1741 gggtccaaca agggcgaagg agttggcaac aagttcctgg ccgatattcg caactgtgat
     1801 gccctcatac atgttctacg ttgcttcgac gaccccaacc tgccccacgt tgatgggtcg
     1861 gttgacccag ttagggatat tgaaacggtg aactttgagc tgcaggtgcg cgatctggag
     1921 tcaattgaga agaagcttca gcgcctcgaa aaggctgcca aggttggcga taaggaggcc
     1981 aaacaggctg ctgccgtgct tggcgtgtac aagaaccacc tggaatcatt ccagaatgct
     2041 gctacggcgc ctgttgacga acaggacaaa aagtatgttg aagacctttt cttgctgtca
     2101 actaaacctg tcatatacgt ttgcaacgta gatgaccaat cggcagtaac aggtaaccac
     2161 tatgttgaca aggtgaggga attccttaaa aacgagcaaa ccgaaatact ggttatagcc
     2221 ggaaagctgg aagctgaaat tgccgaactc gagaatgagg atgatcggtt ggccttcctt
     2281 tccgatgtgg gacttaccga acccggggta aacaagctta tcagggccgc gtactccctg
     2341 ctcaacatgg agacattctt taccgttgga ccaaaggaaa ttcgtgcatg gaccatcaag
     2401 aagggaatga cggctcctca ggctgcaggg gtaattcatt ccgacctgga gcgcgggttc
     2461 attcgtgctg aggttatgaa gtataacgat ttcataaccc tcggttccga gcaggcctgc
     2521 aaggagaagg gtaaactttt tgttgagggc aagaactacg ttgtggagga tggcgacatt
     2581 ctgcatataa ggtttaacgt gtagcagccg gctacattgc tgagcaatat ttttcctgaa
     2641 tcttcttatt aatctggtgc taaaccatca tactttcaat gatgttttag attgtccctg
     2701 tttcctcgaa atgtagttta ttgccgcata aatcttccat tcgacaggta aattttctgt
     2761 tcacttttca aaacaattac ctcctaagct aaagtaaagg ttgaattaca ccatgctttt
     2821 gcgccggtct ttttggcctg gcccttattt gggatgtcgg acaaacacta ctacactacc
     2881 ggcaagatgc aagttcttgc acacgccggg ttgggatgaa gtggtagcga atggcagggg
     2941 tgttactggt ggctgcaaat ctaggagaag ccataagcaa gggcagcgtg cagacgcaca
     3001 caaatccgcc ggtaggcgga caagtttttg aatcctgctt gaattttttt atccttt
//
LOCUS       DAOSHM010000129         3055 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_30287_length_3055_cov_6.925667, whole genome shotgun sequence.
ACCESSION   DAOSHM010000129 DAOSHM010000000
VERSION     DAOSHM010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3055
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30287_length_3055_cov_6.925667"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..874)
                     /locus_tag="PK839_10590"
     CDS             complement(<1..874)
                     /locus_tag="PK839_10590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG73223.1"
                     /translation="MEPINTILHIGYPKTATKWFQNEFYPRVNGIFFIHRKDVFKWLA
                     FQNIFDYSPVETLEHFVSTTDGKRLVICDEIILGGLDIGFGSGEYVCMMANRLKETFG
                     EASIIIFIRNQHSALESVYGHYIKSGGTYSVKKYMGIYRKFTRPFHDYHLFNPDLFQY
                     NKIVELYARLFGSENVHVCLFEDFKANPEGFIDQFSRDFNLSTSTHLNFEPQNIKLST
                     FSVSLMRFSNHFTLKNTVFKHYFFSIPRLYRVLLSVTQMLDKSFLTLHPFTFDKDVHS
                     WIDERFRMGNRELAR"
     gene            complement(879..1772)
                     /locus_tag="PK839_10595"
     CDS             complement(879..1772)
                     /locus_tag="PK839_10595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG73224.1"
                     /translation="MPVIVHIGYPKTATTWLQKRYFPNIKNFNYISFLEVNKLFIDTD
                     FFTFNAQNTRMELLANSKDGNLLLSSEFITTAINAHWHHGYYSAGVAQKIHATFPDAT
                     IVVFIRRQQSLICSAYQQYLKNGGTYGFRRWLYSGDVFCSEHLYYDRMVEFYDNLFGE
                     KQVRVYFYEDFKEDSNGFLTSLNNDLGLVVDMNSISLKPENVGLRRMIIPLLFLVNHF
                     YKKPIGLKRYIIHVPGMTSVGKGIYKFINPLPLFGNFLSEDKMLKPKDKLKLHDFYSE
                     SNRRLANRVGLERLNHHGYYL"
     gene            2165..>3055
                     /locus_tag="PK839_10600"
     CDS             2165..>3055
                     /locus_tag="PK839_10600"
                     /inference="COORDINATES: protein motif:HMM:NF018449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HOG73225.1"
                     /translation="MRKFTTIVLVLLAQVSLAQNWSWVKQFGQSPNPDQTNELDIYDL
                     KFSSNGNIYVTGSYISNVVIGGVTYLGYPDALSIKTDIFLFKLNSDGNVLWVKTMGST
                     ENDLPESIVIDNNENVYLCGRFRSTCDFGGGHTLVSTGGFDSFIAKYNSSGQIQWAIK
                     SAYGGANDRTTAITIGQDSHIYAAGLSKGDFTVGDGTTGDSYTNPDSKNDLFLANYTT
                     NGVYRWSKQIPGNNDNNIFRSIACDEQNNLYVGGALAGTLTVDGIDYTSAGNGDIILM
                     KAHPTDGSAIWVRKGGSTADD"
BASE COUNT          938 a          591 c          637 g          889 t
ORIGIN      
        1 accgcgccag ctccctgttg cccatcctga accgctcgtc tatccagcta tggacatcct
       61 tgtcaaatgt aaaagggtga agggtcaaaa aggatttgtc aagcatttgt gtaaccgata
      121 acagaactcg gtaaaggcgg ggtattgaaa aaaaataatg tttaaacacg gtgtttttta
      181 aggtaaaatg attagaaaat ctcatgaggc taaccgaaaa ggttgaaagt ttaatatttt
      241 gcggctcaaa gttcaagtgg gtgcttgttg acaggttaaa atccctgcta aactgatcga
      301 taaatccttc ggggttagcc ttaaaatcct cgaataagca aacatgaaca ttctcactcc
      361 caaatagccg ggcatacagc tcaactattt tgttgtactg gaacaaatcg ggattgaaga
      421 gatggtaatc atggaatggc cttgtaaact ttctgtaaat tcccatgtat tttttaactg
      481 agtatgtgcc tcccgatttg atgtagtggc cataaaccga ttcgagtgca gagtgctggt
      541 tgcgaataaa gatgatgatg gaggcctcgc caaaggtttc cttcagccgg ttggccatca
      601 tgcaaacgta ctcacccgaa ccgaagccta tgtcgagtcc cccgaggatt atctcatcgc
      661 aaatcactag ccgttttcca tcagttgtgc taacaaaatg ttcgagggtt tcaacaggag
      721 agtaatcaaa aatattctga aaggctaacc atttaaaaac atccttccga tgaataaaaa
      781 aaataccgtt aaccctgggg taaaattcat tttgaaacca cttggtggct gttttggggt
      841 atccaatatg aagaatagtg tttattggtt ccatttgctt acaagtaata gccatggtgg
      901 tttaaccttt ccaatccaac cctgttggcc agtcttctgt tcgactcgct gtaaaaatcg
      961 tgtagcttca atttatcttt gggttttagc attttatctt ccgaaaggaa atttccaaat
     1021 agtggcaagg gattaataaa cttataaatt cctttcccta ccgaggtcat tcctggtaca
     1081 tgaattatgt atcgctttag ccctatgggt tttttataga aatgatttac cagaaaaagc
     1141 aatggtataa tcattcttcg taatcctaca ttttccggtt ttagtgaaat ggaattcatg
     1201 tcgaccacca agccaaggtc attgttcaag ctggttaaaa aaccattact atcctcctta
     1261 aaatcctcat aaaaataaac cctaacctgt ttttctccga aaagattatc gtagaactca
     1321 accatcctgt cgtaatagag gtgttccgaa caaaaaacat caccggagta aagccatcgc
     1381 ctgaacccgt aggttccacc attcttaagg tattgctggt aggcagagca aatcagggac
     1441 tgctgcctac gtataaaaac aacaatagta gcatcgggaa aagttgcatg gattttctgg
     1501 gctaccccgg cagagtagta cccatggtgc caatgtgcat taattgcagt tgtaataaat
     1561 tcggaactta gcagtagatt cccatcctta ctattagcca gcagctccat tctggtattt
     1621 tgggcattga aggtaaaaaa atctgtatcg ataaaaagtt tgttgacctc caaaaaggat
     1681 atgtagttaa aattcttgat attaggaaaa taacgcttct gcaaccatgt ggtggcagtt
     1741 tttgggtatc caatatgaac gataacaggc atgattttga ccagcatata atttgaatag
     1801 gtaaaaatac ctcatttctc atatctttta aataaattgc acgtaattga atggcaaata
     1861 gtccaaaacc gtacaaattt taaattaaga gttacgaagt ggttattgct acacattgta
     1921 ctacaaataa ataagtacat gtggtgtaat ttttgttaaa atttttggac tttttgaatc
     1981 ataagaattt tacatgtttt gcagcagttg tagaatcatg accctatgtg cggttgtttt
     2041 tgggtgagca attcaaaaac aggaggctta ccattaagca aacatcagga ttatttcctc
     2101 taacgatctt atacagttcc atgtaaaaca aagttaagat gttaaaatat taaaccacaa
     2161 agacatgaga aaatttacaa caattgttct ggttttgctg gcacaggttt ctttagctca
     2221 aaactggagc tgggtgaagc agtttggtca atcacctaat ccagaccaaa ctaacgaact
     2281 ggatatctat gatttaaagt tcagcagtaa cggtaatata tatgtaaccg gttcgtatat
     2341 atcaaacgta gttattggtg gagttacata cctaggctat cctgatgcat tgtccataaa
     2401 aactgatatc tttttgttta aactcaatag tgacggcaac gtgctatggg ttaaaaccat
     2461 ggggagcacg gaaaacgatc ttcccgagtc aattgttatt gacaataatg aaaatgttta
     2521 cctatgcggt aggttcagga gtacttgcga ttttggcggt ggtcacacat tggtttccac
     2581 cggagggttt gatagtttta ttgcgaaata taactcatcg gggcaaattc aatgggcaat
     2641 taaatcggct tatgggggtg caaacgatcg tactacagcc attacaattg ggcaggatag
     2701 ccacatctat gctgctgggt tgtccaaggg agattttacc gttggtgatg gaaccaccgg
     2761 ggacagctac accaatcccg attcaaaaaa tgacctcttc ctggctaact acactacaaa
     2821 cggggtttat cgctggtcga agcaaatacc cggaaacaat gacaataaca tttttaggtc
     2881 aattgcctgc gatgagcaga acaacctgta tgtgggggga gcgcttgctg gtacgcttac
     2941 cgttgacggg attgattaca cctccgctgg aaatggagac attatcctga tgaaggcaca
     3001 cccaaccgat ggctctgcca tttgggtccg taagggtggc agcacggccg acgac
//
LOCUS       DAOSHM010000130         3019 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_30777_length_3019_cov_25.499663, whole genome shotgun
            sequence.
ACCESSION   DAOSHM010000130 DAOSHM010000000
VERSION     DAOSHM010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3019
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30777_length_3019_cov_25.499663"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(271..1119)
                     /locus_tag="PK839_10605"
     CDS             complement(271..1119)
                     /locus_tag="PK839_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HOG73226.1"
                     /translation="MVDAIIEFFSYGFVRNAIIASALISILVGFIGTYVVSRRLVFLS
                     GGITHASFGGIGIAWYLGWNPLLGAAIFSIVSAVGIEYLTGEKRLREDSAIGMLWALG
                     MALGVFFVFLTPGYAPDLMSYLFGNILTISTSDNLMLLVLSLVTALFFTLFYYPILFL
                     SFDEEYVKTQHVKARVINFTLKILVALAIVLSIRAIGIILVISLLTIPANIANLFTKR
                     FGNIIWLSALICFGSTILGIILSYRLNVPSGATIVIALALIYLLTRFYFHLKGAIFRD
                     SLRKGL"
     gene            complement(1192..1977)
                     /locus_tag="PK839_10610"
     CDS             complement(1192..1977)
                     /locus_tag="PK839_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG73227.1"
                     /translation="MMSNEHSRIVEIRNVTAGYGRDVVLKNVSLDIYDDDFIGIIGPN
                     GGGKTTFLKLLIGQVKPFSGEVSYSTSGKQLPIGYLPQAGMLDKNFPITVMEVVLSGL
                     LSQKGLTGRYSKAQRQLANDYLRKTGIEALKHRPIGELSGGQLQRTLLCRALISEPKL
                     LILDEPNTYVDTNFEGELYEILKELNQRMAIIIVSHDLGTITSYVKTIACINRTLFYH
                     KSNQLSTELLAAYNCPIQIIAHGPVPHTVLLEHEHHADNNLTE"
     gene            complement(1967..2854)
                     /locus_tag="PK839_10615"
     CDS             complement(1967..2854)
                     /locus_tag="PK839_10615"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HOG73228.1"
                     /translation="MRRISGIMVFLVVVLGLMLPACTEKKHTTSKPIVMVSIQPIKYF
                     VEQIADTLVDVRVMVPPGSSPEMFEPTPGQMVDLSNADIYFAIGLLDFEKGLEPKLKD
                     QLKVRYVNLSGKADLIKEGTEEHDSAGGHHHGVDPHIWMSVEEVRKMSMQILQNLQQL
                     YPAHNDFFSNNYIAFGSKLDSLKEQVNRILGKSSNRAFLVYHPALGYYAREFGLRQIS
                     IEQEGKNPSLKGVVDVVDQARRLGVSAVLSQSQFDIRNAQVIADELNLRVVKVDPLAE
                     DWENSMLHIARAISGEHDE"
BASE COUNT          826 a          677 c          694 g          822 t
ORIGIN      
        1 ttactattgc atacttaatc caactttaca gaaacaaggt tacgtggcgc caacaaaaaa
       61 gctactatat aaaatgaaaa tatctattac ctaaaaattt agggttttgg gttaatgaat
      121 acattcaaag ctgtgcaata gccaatcatc aaagagcttg ataagcaaat gagggcgtgt
      181 agttttttta gaagaatggc atagaaaact ggccaggtta atctggttat ccgattagtt
      241 gtaggtgtga attatgtcgt agtaaggtgt ttacaagcct ttccttagcg aatccctaaa
      301 gatagctcct ttaaggtgaa agtagaatcg tgttaaaagg tagattagtg ccagggcgat
      361 tactattgtt gctcctgagg gaacgttcag cctgtatgaa agaattattc ccagaatagt
      421 tgagccgaag cagattaacg ccgatagcca gatgatgttc ccaaaacgct tggtaaacag
      481 gttagcaatg ttggcaggta tggttaatag cgaaatcaca aggataattc caatagccct
      541 gatggagaga acaatggcaa gggccacaag aattttaagc gtaaagttga tgaccctggc
      601 ttttacatgc tgggttttaa catactcctc gtcaaaggaa agaaaaagaa tggggtagta
      661 gaaaagggta aaaaacaagg cggtaacaag tgatagcacc agcagcatca ggttgtcgct
      721 ggtagaaatg gtgagaatat tgccgaaaag gtacgacatc aggtcggggg catagccggg
      781 ggttagaaaa acgaaaaaca caccaagcgc catgcccaac gcccagagca tcccaatagc
      841 cgaatcctcg cggagacgct tttcgccggt aaggtactca atacccacgg ccgaaacaat
      901 ggagaaaatg gcagcgccaa gcagcggatt ccatcccagg taccaggcaa ttcctattcc
      961 tccgaaggag gcatgagtaa ttcccccgct aaggaataca agcctgcgcg atacaacgta
     1021 tgttccaata aaaccaacca gtatggagat aagcgcagag gcgataattg cattccgcac
     1081 aaatccgtag ctaaagaatt ctatgattgc gtcaaccatt ttgtttttac ctaattgttg
     1141 attatccgca aagtcggcgg aagtttaatt gtggccttta gtttatgttt actattctgt
     1201 taagttgtta tcggcatggt gttcgtgctc cagcaggacg gtatggggca ccggtccgtg
     1261 tgcaattatc tgaatgggac agttgtaggc tgccagcagc tcagtgctta attggttcga
     1321 cttgtggtag aatagggttc ggttgatgca ggcaatggtt ttaacatagg aggtaatggt
     1381 tcctaaatcg tgcgaaacga ttatgatggc cattcgctgg ttcagctcct ttagtatttc
     1441 gtagagttcc ccctcaaaat tggtatcaac gtatgtgttg ggctcgtcga gaataagtag
     1501 ttttggctcg gaaattagtg ctcggcagag caaggttcgt tgaagttgtc cacccgatag
     1561 ctccccaatt ggtcggtgtt tcagcgcttc aattcctgtc tttcttaggt agtcattggc
     1621 cagctggcgc tgagccttgc tgtaacgacc ggtaagccct ttttgggata gaagtccgga
     1681 aagaactacc tccataacgg ttatgggaaa atttttatcg agcatacctg cctgtggcag
     1741 gtaaccaatt ggaagttgtt ttccactggt agagtagctt acctcaccgc tgaagggttt
     1801 tacttgccct atcagcagct tcaggaaagt tgtcttgccc ccgccattcg gcccaattat
     1861 cccaataaaa tcgtcgtcgt agatgtcgag gctgacattt ttcaacacca cgtcacgccc
     1921 atacccggcg gtaacattcc taatctctac tattcttgaa tgttcgttac tcatcatgct
     1981 cgcctgaaat tgcccttgct atatgcaaca tgctgttttc ccagtcttcc gctagcggat
     2041 caaccttaac aacccttagg ttaagctcat cggctatgac ctgggcattg cgaatatcga
     2101 actggctttg ggaaagaacg gccgatacgc ctaacctgcg agcctggtca acaacatcga
     2161 ccacaccctt aagcgatgga ttttttccct cctgctcaat gctaatttgc ctgaggccaa
     2221 actcccgtgc atagtaaccc agtgcaggat ggtaaaccaa aaatgcacga ttggaagatt
     2281 ttccaagtat cctgttaacc tgttccttca ggctatccag cttgctgcca aaagcaatgt
     2341 agttgttgct aaaaaagtcg ttatgggcag gataaagctg ctggaggttc tgtaggattt
     2401 gcatggacat cttccgcacc tcctcaaccg acatccagat atgggggtca accccatgat
     2461 ggtgtccgcc tgcagaatca tgctcttcgg ttccttcctt tatcaggtca gcttttcccg
     2521 ataggtttac ataccttact ttcagctggt cctttagttt aggttccaga cctttttcaa
     2581 aatcgagaag cccgatggca aagtatatgt cggcgttact taaatcgacc atttgtcccg
     2641 gggttggctc aaacatttcg gggctgctgc ccggaggaac cattacccta acatccacca
     2701 gcgtgtcggc aatttgctca acaaagtact taatgggttg aatgctgacc attacaatag
     2761 gcttgctggt ggtatgcttc ttttctgtgc aggccggtag cattaaccct aataccacga
     2821 ccaaaaaaac catgattcca ctaatccttc gcatttcttg ttaatttagc atctacactt
     2881 cgctaccctg cttccaggtt cggttgggct gaacggaccg gttgagacct accggattgt
     2941 tttgaagatg accagaaccc gtgtaaacat caaacaaacc caaattaagc aaatttgttt
     3001 accagacatc ttcttttta
//
LOCUS       DAOSHM010000131         3019 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_30782_length_3019_cov_4.251350, whole genome shotgun sequence.
ACCESSION   DAOSHM010000131 DAOSHM010000000
VERSION     DAOSHM010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3019
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30782_length_3019_cov_4.251350"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="PK839_10620"
     CDS             complement(<1..300)
                     /locus_tag="PK839_10620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG73229.1"
                     /translation="MFFSRKNRQRLFKILGFILVLGNSAGHAQDIESVIKAPMLTNNG
                     GLSFSQIGSYIPGDTLNRINQYSYYISGNMNFSLFSVVNVPMSFAYTNNQLNTEAT"
     gene            complement(307..2361)
                     /locus_tag="PK839_10625"
     CDS             complement(307..2361)
                     /locus_tag="PK839_10625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG73230.1"
                     /translation="MYKKFFVAFLVHLLFASGAVYSQKNGVELLCSPRKDSVLLRWAP
                     TNQKVWKVGNQYGYTVLRYTILRDKKIPKEVEVVQLNSEPLRPRPLTEWEPLADDKYV
                     GIAGECIFNDEYKGVSTDGNPFIVMKKYKNETHRFSFALYAADLSVMAARLSGLYLAD
                     KTAKPNEKYLYKVFIPVPDSIMQVDTASAFTGISEYQPLPKPFDFKAEWADKVVTLSW
                     NFKYLQHIYNSYIVERSEDKGNTYRPIREGGIVPVAEEGVTPELLYVSDTLQSNNLVY
                     YYRVRGISAFGELGPPSDSLFGKGTLPITVAPVIVENSVIDNNKVKLTWTYPEEMNSY
                     ISGFRVYSSSKPKGRKTRIYESKQATERSFVDSTPDITNYYLISVFNPTREKFSSNTT
                     YAELVDSFPPAPPQKLAGFVDSVGRVTVRWSRNTDKDLEGYRVYSSNNPNFEFLLETP
                     GVIPDTVFTDSISIKTLTRNIYYKVRAIDVRQNQSDFSEMLTLARPDVIPPVSPVIKS
                     IAEQDGSIVLSWANSSSDDLAYHRLYRREKSDSLFTLLAKLARTNDKFTTYTDKHVEN
                     GKEYIYYLKAEDSGGLMSKPTKTVSCKTPGVKESIVLKSSGQTGLVKLTWTVKSNKKV
                     ARVIIYRSVNGSPMQLYGNSTTDSYFDNKLSPEKTYEYCIKVIYTDDSSSEFSNTIKV
                     KM"
BASE COUNT          812 a          696 c          629 g          882 t
ORIGIN      
        1 cgtagcctcg gtgtttagct ggttattggt gtaggcaaac gacatgggaa cattcaccac
       61 gctgaacagg ctgaaattca tattccccga gatatagtag ctgtactgat ttatcctatt
      121 cagagtatcg cctgggatat agcttccaat ttggctgaac gatagccccc cgttgttggt
      181 taacattggt gctttaatta cactctcgat atcctgggca tgcccggccg agtttcccaa
      241 aacgagaata aatcctagaa ttttaaataa acgttgccta ttttttctac taaaaaacat
      301 caacttttac attttaactt taatggtatt gctaaactcc gatgaactat cgtcggtata
      361 aataacctta atgcagtatt cgtaagtttt ctcgggactt aacttattat cgaaatagct
      421 atcggttgta gaattaccgt agagttgcat tggtgaacca ttcacgctgc ggtagataat
      481 taccctggcc acctttttgt tggattttac agtccatgtt aactttacaa ggccggtttg
      541 cccgctactc ttaagcacta tgctctcctt aacaccggga gttttgcagc tcaccgtttt
      601 ggttggcttc gacattaagc ctccgctatc ctcggccttt aggtaataga tatactcctt
      661 gccattctca acatgcttat cggtgtaggt tgtaaactta tcgttggtac gggcgagttt
      721 agccaaaagg gtaaatagcg agtcgctctt ctccctgcgg tagagcctat ggtaggccaa
      781 atcgtcgctg gagctgttgg cccagcttag cacaatgctt ccatcctgct ccgcaatact
      841 tttaataaca ggcgataccg gaggaataac atcgggacga gcaagggtta gcatctcgga
      901 aaaatccgat tggttctgcc ttacatcaat agcacgtact ttgtagtaaa tattacgtgt
      961 gagtgttttg atgctgatgg aatcggtaaa aaccgtatcg ggaattacac ccggagtctc
     1021 cagtaaaaac tcaaagttag gattgttgga tgagtacacc ctgtaaccct ccaaatcctt
     1081 atcggtattg cggctccaac gaacagtaac acgaccaaca ctatctacaa agcctgccag
     1141 tttttgcggg ggagcaggag gaaaactatc taccagttcg gcgtatgtgg tgttgcttga
     1201 gaacttctcc ctggttgggt taaataccga tatcaggtag tagttggtaa tatcgggggt
     1261 ggaatccaca aacgaccttt cggttgcctg tttactctcg tatattctgg ttttacgacc
     1321 ttttggtttt gatgagctgt acaccctaaa gcccgatatg tagctgttca tctcctcggg
     1381 gtaagtccaa gttaacttaa ccttattatt atcgataacc gaattctcaa caattaccgg
     1441 ggcaacggta ataggcaatg tacccttacc aaagagcgaa tcagaaggag ggccaagctc
     1501 gccaaaagcg ctaatacccc ttaccctgta gtagtaaacc aaattgttac tctgtagtgt
     1561 gtcgcttacg tagagtagtt ctggcgttac accctcctcg gccacaggaa caatacctcc
     1621 ctcgcgaatt ggcctatagg tgtttccctt atcttcggag cgctccacaa tgtagctgtt
     1681 gtagatatgt tgcaggtact taaaattcca cgatagcgta acaactttat cggcccattc
     1741 agctttgaaa tcgaaaggtt ttggtagagg ttggtactcg gatatgcccg taaaggccga
     1801 tgcggtatcg acctgcataa ttgaatcggg tacgggaata aaaaccttgt agagatactt
     1861 ctcgttgggt tttgccgttt tatcggcaag gtacagcccc gaaagcctag ccgccataac
     1921 ggacaggtcg gcagcgtaaa gggcaaagct aaacctatgc gtctcgttct tgtatttttt
     1981 catgacaata aatgggtttc catctgtgct aacaccctta tactcgtcgt taaagatgca
     2041 ctcgccagca atgcccacat acttgtcgtc ggcaaggggt tcccactcgg ttagtggacg
     2101 tggtcttaag ggttcgctgt taagctgaac cacctcaacc tccttaggga tttttttatc
     2161 gcgcagaatg gtgtagcgca gcacagtgta accgtactgg ttaccaacct tccaaacctt
     2221 ttggtttgta ggtgcccagc gtagcagcac cgaatcctta cgtggactac aaagcagctc
     2281 caccccattc ttctgcgagt aaaccgcacc gctggcaaaa agcaaatgca ctaaaaaggc
     2341 tacaaaaaat tttttataca tatatcgcta tttaacctat ttttaaattt cagccattat
     2401 accccaacca taaaaaacgt ctctctatac ttttgctgcc acacaatact ttattggcac
     2461 agaggtaaat caattcgtct cacaacttct ccggtgtgtc aaaaccattt ctattccgct
     2521 aacgcaagaa ctcctacccc ttctggcaaa gtaagcaggt gttacatgtt caaagttatg
     2581 gggcatgcaa ccctggctaa cgaggtaaag gtagcattat ttttttagaa acttacctgg
     2641 caagctgaca tgattcaaac tttgtcagac gggcagctcg gctctagcag gaaaatttta
     2701 ggggtgttac agccaacgta gctatccccc agggtaaacc catgttaaag gaaaaaaaaa
     2761 tacggattct gcgtcatcaa taccctaaaa ttcgcaagtg aaaaaattgg tttaaaaaat
     2821 ttcgattttt aggtctattt cgaggaattt tattcgtttt tatcgacggt tacgtctctt
     2881 ttcccaaaaa attgtgaatc tgcaaccgcg ggcaatgctt acttctcatc ccacccgatt
     2941 cattaccaca agcaattttt cttctgtgtt taatgcacat aatcaactaa ttaagtttta
     3001 ctcattggca tagcttcct
//
LOCUS       DAOSHM010000132         2977 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_31407_length_2977_cov_8.160164, whole genome shotgun sequence.
ACCESSION   DAOSHM010000132 DAOSHM010000000
VERSION     DAOSHM010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2977
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31407_length_2977_cov_8.160164"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            201..2840
                     /locus_tag="PK839_10630"
     CDS             201..2840
                     /locus_tag="PK839_10630"
                     /inference="COORDINATES: protein motif:HMM:NF013779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C10 family peptidase"
                     /protein_id="HOG73231.1"
                     /translation="MKRLITSLLLLVQFLWLSASPVSINVAKIVATNFLCVQKGLPAD
                     TFQLKLAYTYQLEGRDAFYVFSHSKGGFVIVSADNEAKPIIGYSTTSPATEKIDNPVV
                     ASRFEWYAKQVEHAAKNRLGNKAIQNEWETILFGKTYKKASVGPLLTTTWNQMPYYNQ
                     LCPSNTPTGCVATAMSQIMKYHSWPETGNGWHKYTHTTYGVQYAHFEETTYDWAHMPD
                     VLTGTSSSTSKIAVATLCYHAGVAVNMDYDPEGSGAISNDVMYALSSYFKYDPSTINA
                     YSFDANQQTEWIAKVKTEIDANRPVYYSGSSDASGGHAWVCDGYNDENMLHINWGWGG
                     SYNGYFSADAMNPGTYNFSEYNSIITGIKPGINNQPILWVKQASGFSAPSRGIQYISA
                     VNNRIAWAVAYDGSGSSSNVKDFTLTIDGGSTWKAGTVNATGTTGMGAAMVCAISDKI
                     AWIPLYGSSGGGKIVKTTDGGNTWEHQSTATFTAPNGFPNVVYFWDENNGFCMGDPNN
                     GYFEIYTTINGGNQWTRVPSTSIPANTSEEYGTVGYFDVIGNTVWFSTTKGRIFKSDD
                     KGLTWTAYQTPLSGSFKITFKNESNGIICGLNGEVYEFYKTIDGGETWQQVTPSGDAF
                     TADILYMPGCDTLISAGVDYKNNLMGISYSVDEGTTFTCYANFYKNYQFTALGASPQG
                     AVWAGGFNSDQYNDGMWHKGTLLISGDFKVNKSTVAQNDSTVVFTDQSYGSPETWEWN
                     FGEGASPVTLTGVGPHTVKYTTTGDKTVTLTIQKGEDIQILVMENIVHVTWPDGVNTP
                     TDNQKIQIFPNPVRNNNHISINGFTSGMIDVFNSNGTLVWSSNGMTESNKIYVANLPS
                     GMYIIRIRENNGSITTRRLSVER"
BASE COUNT          961 a          574 c          619 g          823 t
ORIGIN      
        1 ctttcgtgtt ttagtatatg taccaagaag aaattcaaaa atcataccga aaaaatcaaa
       61 caataataca aataaatcaa aaaataaagg aaatttagat atagccattt agtctatttc
      121 taactttata aaaagttaat cgaaacttcc ttggtagatt taaacttcat tttctatctt
      181 taccaaaaaa ccaaatgcct atgaaacgat taattacttc attgcttcta cttgtgcaat
      241 ttttatggct ttctgcaagt ccagtaagca ttaacgttgc caaaatagtg gccacaaatt
      301 ttttatgcgt tcaaaaaggg ttacccgcag atacctttca gttgaaatta gcctatactt
      361 accagctgga aggaagagat gccttttatg ttttctctca cagcaaagga ggttttgtca
      421 tagtctctgc ggacaatgag gccaaaccaa ttattggcta ttcaacaaca agtccagcta
      481 cagagaaaat tgataaccca gtagttgcca gcagatttga atggtatgct aaacaggttg
      541 aacatgctgc taagaacagg cttgggaata aagctattca aaacgaatgg gaaacaattc
      601 tttttggcaa aacatataaa aaggcaagtg ttggcccact gcttacaacc acttggaatc
      661 aaatgccata ctacaatcag ctatgcccaa gtaacacacc taccggttgt gttgcaacgg
      721 caatgtcaca gattatgaaa taccatagct ggcccgaaac ggggaatggt tggcacaagt
      781 acacccatac aacttatgga gtccagtatg cccattttga agaaacaact tatgactggg
      841 ctcacatgcc tgatgttctt actggaacat cttcaagtac atcaaagatt gctgtggcaa
      901 cactgtgcta tcatgcagga gttgctgtaa acatggatta tgacccggag ggttcgggtg
      961 caattagcaa tgatgtaatg tatgcgcttt cctcctactt caagtacgac ccttcaacta
     1021 ttaatgcgta tagctttgat gcaaatcaac aaaccgaatg gattgcaaag gtaaaaaccg
     1081 agatagacgc caatcggcct gtttactact caggaagcag cgatgctagc gggggacacg
     1141 catgggtttg tgacggctac aacgatgaaa atatgcttca tattaactgg ggttggggtg
     1201 gaagctacaa tggttatttt agtgccgatg ctatgaaccc cggaacatat aattttagtg
     1261 agtataacag tataataact ggcattaagc caggcattaa taatcagcca atcctatggg
     1321 taaagcaagc cagtggtttt tcagcacctt ctcgtggtat ccagtatatt tccgctgtta
     1381 acaatagaat tgcatgggca gttgcttatg atggttcagg atcaagttca aacgttaagg
     1441 attttactct aactattgat ggtggaagta catggaaagc aggtacagtt aatgctacag
     1501 gcaccacggg aatgggagcg gctatggtat gtgctattag cgataaaata gcatggattc
     1561 ctctttacgg ttcatcaggt ggcggaaaaa ttgttaaaac cacagatggt ggtaatacct
     1621 gggaacacca gtcaacagcc acatttactg cacctaatgg attccctaat gtggtttact
     1681 tctgggatga aaacaacggt ttctgcatgg gagatcccaa taatggttat tttgagattt
     1741 ataccacaat taatggtgga aaccaatgga caagggtgcc atccacaagc atacctgcaa
     1801 atacatcaga agagtatggt accgttgggt attttgatgt aatagggaat acggtttggt
     1861 tctcaaccac taagggaaga attttcaaat cggatgataa gggattgaca tggactgcct
     1921 atcaaacacc tttatcggga tcattcaaga tcacttttaa aaatgagagt aacggcatta
     1981 tttgtggctt gaatggagag gtatatgaat tttacaaaac tattgatgga ggtgaaacct
     2041 ggcagcaagt caccccaagt ggagatgcct ttacagccga tatactgtac atgcctggat
     2101 gcgataccct aatctctgct ggcgtggact acaaaaataa tctgatggga atatcctaca
     2161 gcgttgatga gggaactacc tttacttgct atgccaactt ttacaaaaat taccaattta
     2221 ccgcccttgg cgcttcacct caaggagctg tttgggcagg aggattcaac tcagatcaat
     2281 ataatgatgg aatgtggcac aagggtacac tcttaatttc gggtgatttc aaagtgaaca
     2341 aatcaaccgt ggcccaaaac gattccaccg tagtattcac cgatcaatcc tacggttcac
     2401 ccgaaacatg ggaatggaac ttcggagaag gagcttcacc tgttacactt acgggtgttg
     2461 gacctcacac agtaaagtac accactactg gggataaaac agttacactc accattcaga
     2521 aaggggagga tatacaaatt ctagtaatgg aaaatattgt tcatgttact tggcccgacg
     2581 gagtaaatac acctactgat aatcaaaaaa tacaaatttt tccaaatccg gtaaggaaca
     2641 ataaccatat atctatcaat ggtttcacaa gcggcatgat tgatgttttt aattcaaacg
     2701 gaactttggt ttggagctca aacgggatga ctgaaagtaa taaaatttat gttgccaact
     2761 tgccatcagg aatgtacata attaggataa gggaaaacaa tggtagtatt accactagaa
     2821 ggttatctgt agaacgatag aactttcact tccgaaagat ttaaaaaaga gaggttttaa
     2881 aacctctctt ttttatagta tttcgttaga tttgagcatc acagcgcccc gttctaccat
     2941 ttcggcaatg gccttttcac catcatctgg attcagg
//
LOCUS       DAOSHM010000133         2920 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_32315_length_2920_cov_7.470506, whole genome shotgun sequence.
ACCESSION   DAOSHM010000133 DAOSHM010000000
VERSION     DAOSHM010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2920
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32315_length_2920_cov_7.470506"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..100)
                     /locus_tag="PK839_10635"
     CDS             complement(<1..100)
                     /locus_tag="PK839_10635"
                     /inference="COORDINATES: protein motif:HMM:NF020463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HOG73232.1"
                     /translation="MQPYFFPYIGYFSLIKHTDEFILLDTVQFIRHG"
     gene            complement(112..1218)
                     /locus_tag="PK839_10640"
     CDS             complement(112..1218)
                     /locus_tag="PK839_10640"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011966987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOG73233.1"
                     /translation="MDRPIYVTKPSLPDLQEFIPYLEQIWESGILTNNGPFHQQLEKE
                     LAEFLGVPYVSLFANGTLALVTALQALRITGEVITTPFSFVATTHSLWWNNIKPVFVD
                     IEPEFCNINPDKIEAAITSKTTAIMPVHVYGNPCRVERIKEIADTYGLRVIYDAAHTF
                     GARYNGQSICSYGDLSILSFHATKVFNTLEGGAIISHDEATKKRVDNLKNFGFVNETT
                     VVAPGINSKMNEVQAALGLVQLKHHPENIEKRKAVAEMYRQSLMGISGISCLSEPEKT
                     EPNYAYFPILVDEHLYGMSRDELYEKLRKNNIYGRRYFYPLISEFSMYKGIDSAEPAN
                     LPVARKMAERVICLPIYPELSKDIVKSIINIIRK"
     gene            complement(1274..2722)
                     /locus_tag="PK839_10645"
     CDS             complement(1274..2722)
                     /locus_tag="PK839_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007662959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOP flippase family protein"
                     /protein_id="HOG73234.1"
                     /translation="MTSLRQKTVSGLSWSFIDSISGQGIQFIVGIILARMLTPREFGL
                     IGMITIFIAVSESFINSGFSSALIRKKECTQADYSTVFFYNLTAGAIFFTILLFSAPA
                     ISGFFKEPELKPIIQVLGAVLIIDSATIIQRTILTKQINFKLQTRISVIAALGSGAMA
                     IGMAYSGFGVWSLVAQRLCKQAINSFLLWLWNKWKPIAVFSRQSFRELFGFGSKLLAS
                     GLIDTIYRNVYYLIIGKFFSAKELGYYTRADQFNALPSQSLTGVIGRVSYPILSSIQD
                     DANRLKASYQQLIRSTMFITFILMVGMAAVAEPMVVGLIGEKWKPSIIYLQMLCFTGM
                     LYPLHALNLNMLQVKGRSDLFLRLEVIKKILAVPVIVIGIFWGIRVMIAGMMLNSVIA
                     LYINSYWSGRMIGYPFRQQISDILPSFSLSLAMAISVYLLGLILTYAPILNLLIQVTA
                     GAAFILVFSEMAKFKDYLFIKEIVMERLHLKR"
BASE COUNT          794 a          640 c          640 g          846 t
ORIGIN      
        1 aaccatgacg aataaattgt actgtatcta ataaaataaa ttcatctgta tgtttaatca
       61 aactaaaata ccctatatac gggaaaaagt aaggctgcat tataccgagt ttcattttct
      121 aataatattt attattgatt taactatatc tttacttagt tcaggataaa tgggtaggca
      181 tataactctt tctgccattt ttctggccac aggtaaattg gcgggttctg ccgagtcaat
      241 gcccttgtac atggagaact cactgattag tggataaaag tatcggcgtc cgtatatatt
      301 atttttcctg agcttttcat acagctcgtc gcggctcatg ccgtataaat gctcatctac
      361 taaaattggg aaatatgcat agttgggttc cgttttttcg ggttcagaca ggcaggaaat
      421 accgctgata cccataaggc tttgcctgta catttctgct actgcctttc ttttttcaat
      481 gttctcaggg tgatgcttca gctgcaccaa tcctagagct gcctgtacct cattcatttt
      541 ggagttgatt cccggggcaa ccacggtagt ttcattcaca aacccgaagt tttttaagtt
      601 gtcaactcgc ttttttgtgg cttcgtcgtg gctaataata gccccaccct ctagcgtgtt
      661 gaatactttg gtggcgtgaa agcttaaaat ggaaagatca ccatagctgc atatactttg
      721 tccgttgtac cttgctccaa atgtatgggc tgcatcatat atcaccctta agccgtaggt
      781 atcggcaatt tcctttattc tttcaaccct gcagggatta ccgtaaacgt gaaccggcat
      841 aatggccgta gttttcgatg tgattgcggc ttcaatttta tcgggattga tgttgcaaaa
      901 ttccggctct atatccacaa aaactggttt tatgttgttc caccaaaggc tatgggtggt
      961 tgccacgaag ctaaagggtg tggtgataac ctcgcctgta attcgtagag cctgcagggc
     1021 tgttaccagc gcaagtgttc cgtttgcaaa cagcgaaaca tagggcacac ccaaaaattc
     1081 ggccagctct ttctccagct gctggtgaaa aggaccgttg ttggttagaa tcccactttc
     1141 ccatatttgc tcaaggtagg ggatgaactc ctgcaggtcg ggcagtgagg gcttggttac
     1201 gtatattgga cgatccatac ttctgctggt atatttttaa cgttacgcat gcttagatat
     1261 acgggttgaa ctattacctt ttcaaatgta acctttccat cactatttcc ttaatgaaaa
     1321 ggtagtcctt aaatttagcc atttcggaga atactagaat aaaagcggcg cctgcggtaa
     1381 cctggattag caggttgagt atgggcgcat aggttagaat taagcccagc aggtaaaccg
     1441 aaatggccat ggctaaagat aaggaaaaag atggaagaat atcggaaatt tgctgtctaa
     1501 atgggtaacc aatcattcta ccactccagt agctgttgat gtacagggca attacggagt
     1561 ttagcatcat tccggcaatc attaccctga taccccagaa aataccaata acaattacag
     1621 gaacggcgag aattttcttt atcacctcca accttaaaaa gaggtcggag cgccccttta
     1681 cctgaagcat gttcaaattc agggcatgta aagggtagag catgcccgtg aagcatagca
     1741 tttgcaggta gatgatggat ggcttccact tttcaccaat taagcccacc accatgggtt
     1801 ctgccacggc tgccatgcca accataagga taaaagttat gaacatggtg cttcggatga
     1861 gctgctggta gctggcttta agcctgtttg catcatcctg gattgacgaa agtattggat
     1921 agctcaccct tcctataact ccggtgaggc tttgggatgg tagcgcgttg aactggtcgg
     1981 ctcgggtgta gtagcccagc tccttggccg aaaagaactt tccaatgatc aggtagtaca
     2041 catttctgta aatggtatca attaacccgc ttgcgagcag cttgctaccg aaaccaaaca
     2101 gctccctgaa cgattgcctg ctgaaaaccg caataggctt ccatttattc catagccaga
     2161 gcaagaacga gttgattgcc tgcttgcaaa gcctttgcgc tacaaggctc cagactccaa
     2221 aacccgaata tgccatgcca attgccatgg cccccgatcc aagggcagct attacagaaa
     2281 tgcgtgtttg aagcttgaag ttgatctgtt ttgtgagaat tgtccgctgt ataatggttg
     2341 cagaatcaat aattagcact gctccaagca cttgtatgat gggttttagc tcgggctctt
     2401 taaaaaatcc gctgattgcc ggcgctgaaa aaagcagtat ggtgaaaaat attgcccctg
     2461 cggtaaggtt ataaaaaaat acggtagagt agtcggcctg tgtgcactcc ttcttccgta
     2521 taagagcgct actaaatcca ctatttataa atgattccga aacggcaata aatatggtta
     2581 tcatgcctat gagaccgaat tctcttgggg taagcattcg ggccagaatt attccgacaa
     2641 taaactgaat tccctgcccg gaaatagaat caatgaaact ccatgacaag cccgaaacag
     2701 ttttctgcct tagggaggtc atttactgaa cttaggcata ttttatgata ttatacgttg
     2761 attcatctac agcttcgccc tgtcagctac accgcctgta tgcgttacac taatatagga
     2821 gagcacggaa atggggcatg tagttctaga atagcctttt aatgtgctcc ttccatgttg
     2881 gcttgggctc atcctcatcg gagtagtagc cactaccccg
//
LOCUS       DAOSHM010000134         2911 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_32477_length_2911_cov_15.411415, whole genome shotgun
            sequence.
ACCESSION   DAOSHM010000134 DAOSHM010000000
VERSION     DAOSHM010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2911
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32477_length_2911_cov_15.411415"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..831
                     /locus_tag="PK839_10650"
     CDS             <1..831
                     /locus_tag="PK839_10650"
                     /inference="COORDINATES: protein motif:HMM:NF013292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HOG73235.1"
                     /translation="EQLATYQNELKNALDIGKRILSGGGSSLDAVEQVVRYLEDCPLF
                     NAGKGAVFTHDGHNELDAAIMDGSNLAAGAVAGVGDVKNPISLAKLVMLKSPHVLLVG
                     KGASEFARLHGATMVDSGYFYTKPRWDDLQRVLEHEKMGTVGCVARDVNGNLAAATST
                     GGMTNKRWGRVGDAPIIGAGTYANNATCAISATGHGEYFIRYTVAHDISALMEYKGLS
                     LNEAALMVVNSKLVKAGGSGGVIGVDSKGNICMVMNTSGMFRAFAKSDGQEGVAVFNK
                     "
     gene            1312..1824
                     /locus_tag="PK839_10655"
     CDS             1312..1824
                     /locus_tag="PK839_10655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG73236.1"
                     /translation="MALNIETKKATGSGLVKIFLLHFFIALLAASFFLFVILLVGREL
                     SWKDFFSYFIIFIPITVVLPLILGIAAASSYKRVEIILTPASSVNLAKLKEFFLKSNY
                     LPAAEESSKFTFKRSNTFRRALCVSSDMPKIQVKGQAVGITMLKRKSATLINQLRNDR
                     RYEVGSEKVE"
     gene            1821..>2911
                     /locus_tag="PK839_10660"
     CDS             1821..>2911
                     /locus_tag="PK839_10660"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOG73237.1"
                     /translation="MMSSGPFILLDSARCRQNIARMEKKARTNGLVFRPHFKTHQSHA
                     VGRWFREVGVNRITVSSAEMAGYFAKDGWNDITIAFPLSRFHIEAVNRLAEDIQLNIT
                     FSSIRNLLDALPLISQNVGVLIEVDVGHHRTGVNISDTRQIALMVNQVAENPKLAFRG
                     FIAHAGQSYSLTGEKNVLAIHNQAMGELVKLKSFWSQQYPEIVISYGDTPTCSLADDF
                     WGVNEIRPGNFVFYDIMQHLIGSCSLDDIALALICPVVDVYPEREEAVVHCGAVHLSK
                     DFVLFPESNRCYGLVCPYNGIRWGAPVEGAYVTSLSQEHGIISFGASGQPFPFHVGDL
                     VAILPVHSCLTLDTMGEMVTESGEKISTM"
BASE COUNT          783 a          563 c          701 g          864 t
ORIGIN      
        1 gaacagctcg caacctatca gaatgaatta aagaatgcgc tcgatatagg taaacgcatt
       61 ttgtctggcg gcggttcatc ccttgatgcg gttgagcaag tagtcaggta ccttgaggat
      121 tgtccgcttt ttaatgccgg taagggtgcc gtttttaccc atgatgggca taatgagctt
      181 gacgctgcaa taatggatgg tagcaacttg gctgctggcg ctgttgcggg tgttggcgat
      241 gtgaagaatc caatatcatt agccaagctt gttatgctca aatctcctca cgtattactt
      301 gttgggaagg gagcctcgga atttgcccgc ctgcatggtg caactatggt cgatagcggc
      361 tatttttata ctaaacccag gtgggatgac ctccaacgtg tgctggagca tgaaaaaatg
      421 ggaacagtgg ggtgcgttgc acgcgatgtt aatggaaatc ttgcggcagc aacttccaca
      481 ggaggaatga ccaacaagcg atggggaagg gtaggagatg ccccaatcat tggcgctgga
      541 acctatgcta ataatgcaac ctgcgcaatt tcagctaccg gacatggcga gtattttatt
      601 cgctacactg tagcccatga tatttctgcc ttaatggagt ataagggttt aagcctcaac
      661 gaagctgcgt taatggttgt taatagtaaa cttgttaaag ctggcggtag tggtggagta
      721 ataggtgttg atagtaaagg gaatatatgt atggtgatga atacttccgg tatgtttagg
      781 gcatttgcca agtcggatgg tcaagaaggt gtagctgtgt ttaataagta ggttaaatta
      841 taaagaataa cgattttctt gctttaaacc tatgctacat tttcgataag cgttaatatt
      901 actggtgata cttttttaac atccccaatt tcctgattac cgatgaggcg ataaaatttc
      961 ttgcaccaag ttgaaggcat caccaagcgt gggcaatggt aaaaatagtt agaatacctg
     1021 aaatgctaaa atttatacgg ttagtagttt ggaattttgc cttgccgatt gcttgcctcg
     1081 cattaattta gatcttagtg ttttttatga tgcctcttaa aaaagagatg gagagtaaat
     1141 tttggaggat ggatttttaa taattgataa ttgaatgaaa caccttttta ggatagcttg
     1201 tataaacaca catgaaaacg aattgggtgt tccatacgct tagctcagat gcctagaaga
     1261 tgtgggccaa aggattgtaa tcagtaacct ataaaatttt aaacagatat tatggcgctt
     1321 aatatagaaa ccaaaaaagc aacaggctca ggtcttgtta aaattttcct acttcacttt
     1381 tttattgcgt tgctagctgc ttcttttttc ctttttgtta ttcttttagt gggtagggag
     1441 cttagctgga aagatttttt tagctacttc ataatcttta ttccaattac tgttgtgctt
     1501 ccgttgattc tgggaattgc agctgcatct tcatacaaac gggtcgaaat catacttact
     1561 ccagcatcaa gcgtaaatct tgcaaagctg aaggaattct tcctcaagag taactacctg
     1621 ccagctgctg aggaaagttc aaagtttacc ttcaaacgct ctaatacttt taggagagcc
     1681 ttatgcgtta gtagcgatat gcctaaaatt caggtaaagg gacaggctgt tggcattact
     1741 atgcttaagc gaaaatcagc taccctgata aatcagctta gaaatgatag gcgctatgag
     1801 gttggttctg aaaaagttga atgatgagta gcggtccatt catacttctg gatagtgccc
     1861 gatgcaggca aaatattgcc cgcatggaaa aaaaggctag gactaatggt ctggtattta
     1921 ggccacattt taaaactcat cagagccacg ctgttggccg ctggtttcgc gaggtagggg
     1981 ttaacagaat taccgtttca tcggctgaaa tggccggata ttttgccaag gacggatgga
     2041 acgatattac catagcattt cctctatccc gtttccatat agaagctgta aatcgtcttg
     2101 ctgaggatat acagctgaat atcacctttt catcaattcg aaatttgctg gatgcgctac
     2161 ccctgattag ccaaaatgtg ggtgtactca tcgaggttga tgttggccat catcgtacgg
     2221 gtgttaatat ttcggatact cgacagatcg ctctaatggt gaatcaagtt gcggagaacc
     2281 ctaagctggc ttttcgcgga tttattgccc atgctggcca gtcctacagc ctaacaggtg
     2341 aaaaaaatgt tttagctatt cataaccagg ccatgggcga gctggttaag ctaaagtcgt
     2401 tctggagcca gcagtaccct gaaattgtca tatcatacgg cgacacaccc acctgttcgt
     2461 tggccgatga tttctggggt gtcaacgaaa ttcgccccgg taacttcgtt ttttacgata
     2521 ttatgcagca cttgattggc tcgtgcagcc tggatgacat tgccctggcc ttgatttgtc
     2581 cggtggttga tgtttatccc gaaagagagg aggcagttgt ccactgtgga gccgtacact
     2641 tgtcgaaaga tttcgttctt ttcccggaaa gcaacaggtg ctacggcctg gtttgtccct
     2701 ataatggtat aaggtgggga gcgccagtag agggagccta tgtaacctcc ctgagccagg
     2761 aacacgggat catctccttt ggtgcgtcgg gacaaccttt tccttttcat gtaggtgatt
     2821 tggttgccat tctacctgtc cacagctgcc taacgctcga taccatgggt gaaatggtta
     2881 ccgaaagcgg tgaaaaaatt tcaaccatga g
//
LOCUS       DAOSHM010000135         2892 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_32776_length_2892_cov_20.019034, whole genome shotgun
            sequence.
ACCESSION   DAOSHM010000135 DAOSHM010000000
VERSION     DAOSHM010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2892
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32776_length_2892_cov_20.019034"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1370)
                     /gene="rlmD"
                     /locus_tag="PK839_10665"
     CDS             complement(<1..1370)
                     /gene="rlmD"
                     /locus_tag="PK839_10665"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009292431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HOG73238.1"
                     /translation="MSKDKYPIIEKVRIEDIAAEGKSIARIDGRVLFVPYAVPGDLVD
                     VQLTRKRKNYMEGYVVRYHEYSPIRVEPFCRHFGTCGGCKWQNLPYAEQLKIKQRQVE
                     DQLRRIGRVNFPTVSPILGSERTTGYRNKLEFTFADRRWFSGDELAKADKLPDEPALG
                     YHIPGRFDFVFDVEECFLQPEPSNSVRLAVKNYAADHHIPFMNLRSHEGTLRNLIVRT
                     SSTGDVMVILSLRQLSSDVEQLLDFMVEKFPQVSSFNYVVNAKLNDTINDLEVVCYHG
                     NKYITEEMEGIKFRVGPKSFYQTNSEQACVLYGVVREFAGLTGDELVYDLYTGTGTIA
                     NFLARNARRVVGMEYISEAIADAIENSKLNGITNTSFFTGDIKDLLTDDFVIHEGNPD
                     VVVLDPPRAGVHPDVLKALLKISPLRVVYVSCNPATQARDIALLDSDYAIKRVQPVDM
                     FPHTQHV"
     gene            1436..2440
                     /locus_tag="PK839_10670"
     CDS             1436..2440
                     /locus_tag="PK839_10670"
                     /inference="COORDINATES: protein motif:HMM:NF016214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HOG73239.1"
                     /translation="MDSLTHIVLGAVVGEAAVGKKMGNKALVYGAIAGSLPDFDVFVA
                     RFFDPVDALFVHRGFSHSLFLLVILAPLAAYAVKHFSHNPRLTMLNWLALIGLPWLSH
                     ILVDIFNTYGTAILEPFSHARISFDSVPIVDIFILLLLAAAVVVILLAKRKNVLRKMA
                     AWASLSLTIAYFGTSAIIKTCVDGETRKSLMAKGATYTRIHSTPLPLTNLIWMVVVED
                     SSGYTISQKNLINGKTTHKAYLARNEGLLKGCENNNNMGKLLNFTRGFYTVEKSSNEH
                     LKIYDLRFSSLAGVYPQAFVFMFEVNCNDGKLLDHPAHPSRGIIPRNVKNLWNSLWQS
                     "
     gene            complement(2437..2850)
                     /locus_tag="PK839_10675"
     CDS             complement(2437..2850)
                     /locus_tag="PK839_10675"
                     /inference="COORDINATES: protein motif:HMM:NF023390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS/SEC14 domain-containing protein"
                     /protein_id="HOG73240.1"
                     /translation="MTPIKVFDSSYALVTFDIERKIIKVIWNGNHTVSEYKAAIGEAL
                     NFQRVSGFPVYTYLSDIRNQGIVNPESRKWFERVAIPQAVNGGLRRAAVVYDGSVFKN
                     YYLNLILLAANVYQLPLKFFGSMEEAYEWIESFHD"
BASE COUNT          796 a          699 c          621 g          776 t
ORIGIN      
        1 acatgttggg tatgtgggaa catgtcaacc ggttgtacgc gctttatggc ataatctgaa
       61 tcaagcaggg caatatccct ggcctgcgtt gcaggattac aactcacgta aactactcgt
      121 aggggagaaa tcttgagcag tgctttcagc acgtcgggat gaacaccagc cctgggagga
      181 tcgagcacca caacatctgg atttccctca tgtatcacaa aatcatccgt aagcaaatcc
      241 ttaatatcac cagtaaaaaa acttgtatta gtaattccgt ttagctttga gttctcaata
      301 gcatcggcaa ttgcctctga aatatactcc attccaacaa ccctacgggc gttacgggct
      361 agaaagttgg caattgttcc agttccggtg taaaggtcgt acaccagctc atcgccggta
      421 agtcctgcaa actcccttac aactccgtaa agcacacatg cctgttccga gtttgtctgg
      481 taaaacgatt ttgggccaac cctaaacttt atgccctcca tttcctcagt aatgtattta
      541 tttccatggt agcacactac ctccaaatcg tttatagtat cgttcaactt ggcgtttaca
      601 acgtagttga acgacgagac ttggggaaac ttttcaacca tgaagtcgag cagctgctct
      661 acgtcggagc taagctgcct caacgacagg ataaccatta catcaccagt tgacgaagta
      721 cggacaataa ggttacgaag tgtgccctcg tgcgaacgaa gattcatgaa cggaatatgg
      781 tggtcagcag cataattttt aacagcaagc ctaacggagt ttgatggttc gggttgcagg
      841 aaacactcct ccacgtcgaa cacaaagtcg aaacgaccgg gaatatggta tcccagcgca
      901 ggctcatcgg gtagcttgtc ggctttggcc agctcatcgc cactaaacca gcgtctatcg
      961 gcaaaggtaa attcgagctt gtttcggtaa ccggtagttc tttctgagcc tagaatgggc
     1021 gaaacggttg gaaaattgac ccttccaatt cgtcgaagct gatcctcaac ctgtctttgc
     1081 tttattttga gctgctcggc atagggcagg ttttgccact tgcacccgcc gcatgttcca
     1141 aaatgcctgc agaatggttc aacccgaatg ggcgaatact catggtaacg aaccacataa
     1201 ccctccatgt agtttttccg ctttcgggtt agctgaacgt caaccaaatc gcccggaaca
     1261 gcgtagggaa caaacaatac cctaccgtca atcctggcaa tagatttacc ttcggcagct
     1321 atatcctcaa ttctaacctt ttcgattatc gggtacttat ctttactcac tttatcgtgc
     1381 tatatttgaa ccaacatcaa cattggctgt tagggtgcaa aggtagcaaa ttgttatgga
     1441 ctcactaacc cacatcgttt tgggcgctgt tgttggagaa gcagcagtag gtaaaaagat
     1501 gggcaacaag gctttggttt acggggctat agccggaagt ttacccgact tcgacgtttt
     1561 tgtagccaga tttttcgacc ctgttgatgc cctgttcgtg catcgggggt ttagccactc
     1621 cctctttcta cttgtaatcc tcgccccatt agctgcctac gcagtaaaac atttctccca
     1681 taaccccagg ctaaccatgc tgaattggtt ggcgctgatt ggcctaccat ggctgtcgca
     1741 catcttagtt gatattttta acacctatgg aacagccatc ctcgaacctt ttagccatgc
     1801 cagaatttcg ttcgactcgg tacccatcgt ggatattttc atcctacttt tactggcagc
     1861 tgccgtggtg gtaattttac ttgcaaaaag gaaaaatgtt ctgcgtaaga tggctgcctg
     1921 ggcgtcactt tccctaacca tagcctattt tggcacttcg gctataatta aaacatgtgt
     1981 tgacggtgaa accaggaaaa gccttatggc caaaggtgca acctatacac ggattcatag
     2041 cacgcctctc ccactgacaa acctaatatg gatggtggtg gttgaggata gcagcggata
     2101 caccatcagc caaaaaaatc taattaacgg caaaactacc cacaaagctt acctggctcg
     2161 aaatgaaggt cttcttaagg gctgtgaaaa caacaataac atgggtaagc tgctgaattt
     2221 cacaaggggt ttctacactg tggaaaaatc aagcaatgaa catttaaaaa tttatgattt
     2281 acggttctct agtcttgcag gcgtttatcc ccaagcattc gtattcatgt ttgaagttaa
     2341 ctgcaatgat ggtaaattac tggatcatcc cgctcacccc agcaggggaa taatcccccg
     2401 taatgttaaa aatctatgga acagcctatg gcagagctaa tcgtgaaaac tttcaatcca
     2461 ctcataagcc tcctccatag agccgaagaa cttcaagggt agctggtaaa cgttagccgc
     2521 cagcagaatc agattaaggt agtagttctt gaacacgcta ccatcataaa caacagcggc
     2581 tctgcgtagc cctccattaa cggcctgggg aatggctacc ctttcaaacc atttacggct
     2641 ctcgggatta acaataccct gatttcggat atcggacaag taggtgtaaa ccgggaaccc
     2701 gcttaccctt tgaaaattaa gggcttcgcc aatggctgcc ttatactcgc taacggtatg
     2761 gttgccattc catataacct tgataatttt tcgttcgata tcgaaagtta caagagcata
     2821 ggaagaatcg aaaaccttaa ttggggtcat gttttttaag taagttacca ccctacagct
     2881 aaggagtcaa ct
//
LOCUS       DAOSHM010000136         2847 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_33529_length_2847_cov_20.015043, whole genome shotgun
            sequence.
ACCESSION   DAOSHM010000136 DAOSHM010000000
VERSION     DAOSHM010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2847
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33529_length_2847_cov_20.015043"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="PK839_10680"
     CDS             complement(<1..458)
                     /locus_tag="PK839_10680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG73241.1"
                     /translation="MPYHLLLIGMGIGVVYIMGSLLVYLGILSKNLHRKIWNYTLLAV
                     FLGCVVLGLLLAIQISYKLDWPFISSALKWHVNLGIAMSLVAAIHIFWHLRYFTGSSH
                     RIHKKISQNECSTLVTTHELGIMAFAAGLASISFQILILRQVTKVFQGNE"
     gene            complement(476..1942)
                     /gene="amrB"
                     /locus_tag="PK839_10685"
     CDS             complement(476..1942)
                     /gene="amrB"
                     /locus_tag="PK839_10685"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HOG73242.1"
                     /translation="MESSCKGQTGGSSRGVDRQPAVAGSFYPASREQLVKDLQGYFDA
                     AEVSLTSQPIALIVPHAGYVFSAPVAAAGFKQLDRERKFKHIFLIGSSHTMYFNGVSA
                     YTQGHFITPLGRVKVDDLAKELADRYDFINDDPMPHEREHGLEVQLPFLQYWLKHEFC
                     IVPLIIGGESQSTSTLLAEVLAPYLNEENLFVISSDFSHYPTYAEATKSDKSMADAIV
                     SNSSSAFMKAKLNSENSGADNLVTAMCGWTSGLTLLKMTEKIKGITYQPLIYMNSGDS
                     PYGEKDRVVGYWAIAAVRESNRSGDFSLTEEDKMELLKLARKTITDHLNGLKPTPPDL
                     QNLSANLKTNAGAFVTLRRDGQLRGCIGSFSTTTPLYQVVMAMAVAAATEDYRFPPVT
                     RNELGEVEIEISVLTPMKKISSIDEIELGRHGIYIRKGGHSGTFLPQVAAETGWSLEE
                     FLGHCARDKALIGWDGWRDAEIFTYEAIVFEEKDFKMN"
     gene            2316..2726
                     /locus_tag="PK839_10690"
     CDS             2316..2726
                     /locus_tag="PK839_10690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG73243.1"
                     /translation="MPAKKKEILTVEQGRPFMFIALSTTLNLNKLLWAASNHCGLRLV
                     RNAELEQLIGVSSFTDRTSKPGLVVSLFPNRSSNGLVVGQLPNVDYIVEINGNAAEAE
                     VKILSACLRGFQGIQAAITINPSKIKISEPFCPE"
BASE COUNT          813 a          667 c          637 g          730 t
ORIGIN      
        1 aactcgttac cctgaaaaac cttggtaacc tgtcgcagaa ttagtatctg aaatgaaatg
       61 gaggcgaggc ctgcggcaaa tgccataatg cccaactcgt gggttgtaac caaagtagag
      121 cattcatttt gactgatttt cttgtgtatg cgatggctgg atcccgtaaa gtaccgtaaa
      181 tgccagaata tatgaatggc tgctaccagg ctcatggcaa taccaaggtt aacatgccat
      241 ttcagggcag aggaaataaa tggccaatca agcttgtagc taatttggat ggccagcaac
      301 agacccagca ccacacaacc aaggaataca gccagaaggg tgtagttcca aattttacgg
      361 tgaaggtttt tggagagtat gcccaggtaa accagcaagc tgcccataat gtaaactacg
      421 ccaataccca tgccaatcag cagtaaatgg tagggcatat tcataaccgg cagatttaat
      481 tcattttaaa atccttttcc tcaaacacaa tggcctcgta ggtaaaaatc tcggcatccc
      541 tccatccgtc ccagccaata agagccttgt cgcgtgcaca atggccaagg aattcctcaa
      601 ggctccagcc tgtttcggcg gcaacttgtg ggagaaatgt tccggagtgt ccgcctttcc
      661 ggatgtatat gccatgcctg cccagctcta tctcgtcaat gctgctaatt tttttcattg
      721 gggtaagcac cgaaatttca atttcaactt caccaagctc attacgggtt acagggggaa
      781 aacggtaatc ctccgtagcg gcagcaacgg ccatggccat aaccacctgg tagagtggag
      841 tagtagtgga gaagctgcca atacaacccc taagctgtcc gtccctgcga agagtaacaa
      901 atgcaccggc attggtcttc aggttagcgc tcaggttctg caaatcggga ggtgttggct
      961 ttaagccgtt gaggtggtcg gtaatggttt tacgcgccag cttaagcagc tccattttgt
     1021 cctcttcggt caaagaaaaa tcaccagacc tattgctttc cctcacggca gcaatggccc
     1081 agtaacccac taccctatct ttttcgccgt atggcgaatc acccgaattc atgtagatga
     1141 ggggttggta ggtaatccct ttaatttttt cggtcatctt cagcagggtt agccccgaag
     1201 tccaaccaca catggcggta accagattgt cagcaccaga gttttcagag ttcagcttag
     1261 ctttcatgaa tgctgaagac gagttcgaaa caattgcatc agccatgctt ttgtcggatt
     1321 ttgtggcctc agcatatgtc gggtaatgcg aaaagtctga acttattaca aacaggtttt
     1381 cctcgtttag gtatggggca agaacttcgg cgagcagggt gctggtactt tgcgattcac
     1441 caccgatgat aagaggaaca atgcagaatt catgcttaag ccagtactgg agaaagggga
     1501 gctgcacctc cagcccatgc tcccgctcgt ggggcatggg atcgtcgttg ataaaatcgt
     1561 acctatcggc cagctccttt gccaggtcgt caaccttaac acgcccaagg ggggtgataa
     1621 aatgaccttg ggtatatgct gatacaccgt tgaagtacat ggtgtggctg gagccaatca
     1681 aaaaaatatg cttaaacttc ctttccctgt cgagctgctt aaaacctgca gctgcaacag
     1741 gcgcggagaa aacgtacccg gcatggggaa ctattaaagc tattggttga gatgtaaggc
     1801 taacctcagc cgcatcaaaa taaccctgca aatcctttac cagctgctcc ctggaggcag
     1861 ggtagaacga tccggcaact gcaggttgac ggtcaactcc cctactactt ccacccgttt
     1921 gccctttaca tgacgattcc attgctacta gtgttattag taaacttatc gttacaaaat
     1981 atcgtgattg aaagcccggc atgatagcac gaatatttac ctttcccatt caaagttatt
     2041 tcaaaataaa agaaaaatca agttcatatc actccagcca gctaccataa gacaactttg
     2101 ggaaaagttg ctaaaaccta ttcaaattcc cgatggttag aaggctaacg gttggtcggg
     2161 aatagggtaa gcgaggagtt gaaattcaga aatgctaaaa attaacccgt tcgcattcag
     2221 cttccaacac cgtccgattt cgttaagata aatttagggg ttcggtaaga ttgtttaaaa
     2281 acagggttat atttgtgcta gactagtaag ctaaaatgcc ggcaaaaaag aaggaaatac
     2341 ttactgttga gcagggcagg ccttttatgt ttattgcact gtccaccacg cttaacctaa
     2401 ataaactact ctgggctgct agcaaccatt gtggcttaag gttggttaga aatgccgagt
     2461 tagaacagct cataggtgta tcctcgttta ccgacagaac aagcaaacca ggcttagttg
     2521 tctctctttt tccaaaccga agcagcaatg gtcttgtagt tggccagcta ccaaatgttg
     2581 actatatagt tgaaataaac ggaaatgctg cagaagctga agtaaaaatc ctatccgcct
     2641 gtctcagggg ctttcagggc atacaggcag ccattaccat taacccatca aaaataaaaa
     2701 taagtgaacc cttttgtccg gagtaaacaa gttgctggtc tccaaattct cagcgctaac
     2761 ccacaagtcc aaggctacca ccaacaaaac ctttgttaaa ctgtgttaag gtaacaccct
     2821 gcaaaatcat tcccgttatc tttgttt
//
LOCUS       DAOSHM010000137         2818 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_34044_length_2818_cov_18.028592, whole genome shotgun
            sequence.
ACCESSION   DAOSHM010000137 DAOSHM010000000
VERSION     DAOSHM010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2818
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34044_length_2818_cov_18.028592"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            53..391
                     /gene="rbfA"
                     /locus_tag="PK839_10695"
     CDS             53..391
                     /gene="rbfA"
                     /locus_tag="PK839_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010535697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HOG73244.1"
                     /translation="MEGPRLQKVDRLVQKELGDIFQREGQARLRGKIITVTRVRVSPD
                     LSLAKVYISIFPSKEGAQTLELLNEHKKAIRGELGRRVRHQLRIVPELAFFIDDSLDY
                     IEHIDDLLKS"
     gene            405..1178
                     /locus_tag="PK839_10700"
     CDS             405..1178
                     /locus_tag="PK839_10700"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOG73245.1"
                     /translation="MMRQIISLFTRFVPRHRLQVFAAFVLRVVGIFYRGNRYEDPISG
                     KTYRKLLPYGRTVVRPNALAPHSMSLERHRLIWLYLRERTDFFTAPHSLLHIAPEYCF
                     LKPFRKLGNIEYVTADLISPWADVKLDVQAMPFPSSQFDVVICNHVLEHVQSDIKAMG
                     EIYRVLKPGGFAILQVPIDISREKTLEDPSINTPELREQYYLQRDHQRLYGRDYADRL
                     RSVGFRVDENHFVDELPLNLVERYALPVGEVLFICNKPD"
     gene            1267..2502
                     /locus_tag="PK839_10705"
     CDS             1267..2502
                     /locus_tag="PK839_10705"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOG73246.1"
                     /translation="MHLPIYIARRYLFAKKSHNVINIISIISLVGVATGVMALVVVLS
                     VFNGFDTLIERLYSNIDADIKILPREGKTFSITGEKFDKLAKMNGVAVFAEIFEENAL
                     FSYRGKQHIGIIKGVSRNYSELTGLDSMMKDGEFKLWMGSQPMAVVGQGVAYYLNANL
                     AQFDPLFVYIPKRGRASLTMESAFGKKAIMPSGIFSVEQDFDTQYVIAPVEFVRNLLG
                     YDSTMVTAIEVKVAGGVDAESIRRSMEEVLGSDFRVLNRYQQNESLYRTMKSEKFAIG
                     LILTLILVIASFNIVGSLSMLIIDKRKDVETLRSLGADNGLIKKIFMVEGMLISIGGT
                     IIGTLLGLLICWLQVHFKLITLEGRGNFIIDAYPVDIQLPDIGAIILVVLLIGYLAAR
                     FPVRMITRRILNEDIRSNE"
BASE COUNT          682 a          683 c          727 g          726 t
ORIGIN      
        1 ttgaagtttt acgatatcaa cttcatcatg tatctttgcc aaaaagagag ctatggaagg
       61 accaaggctt caaaaggttg accggctggt tcaaaaggag ctgggcgata ttttccagcg
      121 ggaggggcag gccaggctac ggggcaaaat aattactgta acccgtgtta gggttagccc
      181 cgacctgtcg ttggccaagg tgtacataag cattttcccc tccaaggagg gtgctcaaac
      241 tcttgagctg ctgaatgagc ataaaaaggc catacgtggt gaacttggta ggcgtgtgcg
      301 ccatcagctg cgcattgtgc cagagctggc attttttata gacgactcgc tcgactacat
      361 cgagcatatt gatgatctcc taaaatcata atagggccac cggtatgatg aggcaaatca
      421 tttcgctgtt tacccggttt gttccccgac atcgccttca ggtttttgct gcattcgtgc
      481 ttagggtggt gggtattttc taccgcggca accgctatga ggatcccatc tctgggaaaa
      541 cctaccgcaa gctgctgccc tacggtcgca ctgttgtcag gccgaatgcc ctggcgcccc
      601 actccatgtc gctcgagcgt caccggctta tttggctata cctgcgtgag cgcaccgatt
      661 ttttcacggc tccgcatagc ctgcttcaca ttgcaccgga gtactgcttc cttaaaccct
      721 tcagaaaact aggtaacatt gaatacgtga ccgccgatct gatctccccc tgggcagatg
      781 ttaagctcga cgtgcaggca atgccattcc ccagctccca gttcgacgtg gtaatctgca
      841 accatgtgct ggagcatgtg cagtccgaca ttaaggccat gggggagatt tacagggtgc
      901 ttaaacccgg aggttttgcc attcttcagg tgccaattga tatcagcagg gagaaaaccc
      961 ttgaagaccc ctccatcaac accccggagc tgcgcgagca gtactaccta cagcgcgacc
     1021 atcaaagact ttacggtagg gattatgcgg atcgcctgcg aagtgtgggc tttagggttg
     1081 acgaaaatca ctttgtggat gagttgccgt taaacctcgt tgaacgctat gccttgcctg
     1141 tgggcgaggt gcttttcatc tgcaataagc cggactagat aattaataat gtgaagagtt
     1201 ttgctccata tcaatcaaat tataaggtat atattacgcc tttagccaaa agcatttgct
     1261 aaggatatgc acctgcccat ttacattgcc cgtcgttacc tctttgcgaa gaaatcgcat
     1321 aatgtcatca acatcatctc catcatttcg ctggtagggg tggcaactgg ggtaatggcg
     1381 ctggtggttg tgctttcggt gttcaacggg ttcgacaccc ttatcgagcg gctttactcg
     1441 aatattgatg ccgatattaa gattcttccc cgtgagggta aaaccttcag cattaccggg
     1501 gaaaaattcg acaagctggc aaaaatgaat ggagtagccg tttttgctga gatttttgaa
     1561 gaaaatgccc tattcagcta ccgtggcaag cagcacatag gcattattaa aggcgtaagc
     1621 cggaactaca gcgagcttac cggactcgac tccatgatga aagacggcga gttcaagcta
     1681 tggatgggta gccagcccat ggctgtggtg gggcagggtg tggcctacta cctcaatgcc
     1741 aacctggcgc aattcgaccc cctttttgtt tacattccca aaagagggag ggcatccctt
     1801 actatggaat cggcttttgg gaagaaggcc atcatgccgt cgggaatttt ctccgttgag
     1861 caggattttg atacgcagta cgtcattgcg ccggttgagt ttgtgcgaaa cctgctgggt
     1921 tacgactcca ccatggttac tgctattgaa gtcaaagtgg ccggcggggt ggatgccgag
     1981 agcatcagga ggagcatgga ggaggtactg ggcagcgatt ttagggtgct gaaccgctac
     2041 cagcagaacg agtcgctcta ccggaccatg aagtcggaga agtttgccat agggcttatc
     2101 ctaaccctga tactggtgat tgcctcgttc aacatcgtgg ggtcgctctc catgcttatc
     2161 attgataaac gcaaggatgt tgaaaccctc cgcagcctgg gtgcagacaa cggccttatt
     2221 aaaaaaatat tcatggttga gggcatgctc atttccattg gcggaacaat tatcgggaca
     2281 cttttgggac tgctcatttg ctggcttcag gtgcatttca agttgattac ccttgaaggt
     2341 cgaggcaact tcatcattga tgcctacccg gttgatatcc agctgccgga cataggggca
     2401 attatcctgg tggttctcct gattggctac ctggcagccc gattccccgt gcggatgatc
     2461 acccgccgca tactgaatga ggatatccgt agtaacgagt aggctttggg cattttttgg
     2521 gcggtttaca tgcttgccag gcacagccct ctgaccacat ttcccttccc ggcttcagcc
     2581 tctccgccca tgtccacaaa accaaatgcc agggtgacga ccctagggct tagtatgggt
     2641 cgcagcattt agccaggaat tgcaccccag ggatagccaa aaccatgtgt aaaaagggta
     2701 attctgtgag tggcagctta ccaggttaat caaaaaaatc aggtaaaatt tgattttttc
     2761 aaaatcaaat ggtaaagttg tttaccagaa cagcggctat gagcagcttt tcacggtg
//
LOCUS       DAOSHM010000138         2765 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_34998_length_2765_cov_4.279336, whole genome shotgun sequence.
ACCESSION   DAOSHM010000138 DAOSHM010000000
VERSION     DAOSHM010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2765
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34998_length_2765_cov_4.279336"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1285..>2765)
                     /locus_tag="PK839_10710"
     CDS             complement(1285..>2765)
                     /locus_tag="PK839_10710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG73247.1"
                     /translation="LNTEATLPFNRFSIAPSYKWITLYAGYASMTFSPYTLVGHETFG
                     GGVELKFDNGFKFSAIYGRLQKEVRPDSAINSAAFRRMGGGFKVEYSHEKVDVALNIY
                     KAKDVNNNDFYTSTIDSTLTPKDNISGSLYLNMKMINNFQLNMEYAISAINADISRSD
                     SVDRSFKDNFLESNGDLSVHHALKAGISQTSAIGTVGATYERISPNYTTLSAYYFLND
                     FENITANYSTTIRKIVNIAIDAGYQHDNLSKQKVNANNRFIFSGNVSASITKRLSVGA
                     SYSNLKSYVHIRDIYNDLTNTNPYQNIDTLSFTQLNLTTSGNASYTLRSTDENRQNII
                     VSFTYQDASEQQNRTEKYSGSKIYNAVMSYQFSQIPQKFNASTSVNYNHNNLPNGFMG
                     VLSYNLSLQKTFIEKLKLAIIGTYSRSFNDSLNLANVVNIRFSAGYTIQKRHNLNLSI
                     AHVSSQNKLRDNTQYTANMSYSYMFDFNLRRKDKKLNFEGNF"
BASE COUNT          823 a          552 c          516 g          874 t
ORIGIN      
        1 tacgtctctt ttccccaaaa attgtgaatc tgcaaccgca gcaatgctta cttctcatcc
       61 cacccgattc ataaccacaa gcgatttttt cttctgtatt taaaacacag ataatcaact
      121 aattaagttt tactcaaggg catagcttcc cccttgttgc tcaatcggtt aatcaggttt
      181 actcaaatac caggtgatat tttttgtagt gaaaatttta atacataact cctgccttgt
      241 tttatccatt ttgaggggac aaccataaaa cggaaaatag attttttcag tctgaagttt
      301 ggcttgataa aatcgagttt acggcttata aattcaacaa gaaagtggta caggtagcgg
      361 caaattgcca tcatcaaaag aaacacggta ttttcgtgca aaaatgaaaa cggcatctat
      421 tcccatccca caaaaagtca ttgttcatct catcgaacag gcgttcgctt tcacccctgt
      481 cgttataaaa ctcaacgacc attatcaccc cccccacttg gtgatgtact ccgcacattc
      541 tccgccgcaa agcgtcagta aaaattacta acgaaaaagt tcactataag tgtattacgc
      601 cttaacatct agattaccaa gcatttgatc gattgttttg taaaccgaga cgttcccgac
      661 aagtgaatct gcaaaatttg ttccgccaaa tgaattgata tttgaagtag aatagctaac
      721 tttgaacatg gggttgaact tttcaactaa ataagtgaaa aatttttatc gggcaagccc
      781 tgaaactctt ttttttacgg ggccctccgt ttttttttta acattctttg atgcggattc
      841 gaggagtaat attaaatggc actttgtgaa ctttgtgtag tatcttggcg acctttgtgg
      901 taaaaaaatt aacgtaaata agtgactttt gttttactat tacatactta atccaatttt
      961 actgaaacaa ggttacgtag cgactttgtg ctctctatgc tttctttgtg gcctttgtgg
     1021 ttaaatataa ttttgtacct atgaatgcaa tctggtatta agtcggtaag gaaaataaaa
     1081 tttgaaccta atgaatcggt tattgtcgaa attaataaac caacaaaaaa actactggag
     1141 aaaatgaaaa tatctattac ctaaaaattt aggatttggg attaacaaac ctatcaggat
     1201 ttatacccac gaacttgcct acattaaaaa tgactcctat aggagaaaga tcaaaaattg
     1261 ccgttgaggt atagccaaaa aaacctagaa attcccctca aaatttagct tcttatcctt
     1321 tctcctcagg ttgaaatcga acatatagct gtaggacata tttgcggtgt actgggtgtt
     1381 atccctcaac ttattttggc tcgacacatg agcaatgctg aggtttaggt tgtgtcgttt
     1441 ttgaatggta taacctgccg aaaatcttat atttacaaca ttggccagat tcaatgagtc
     1501 gttaaacgaa cggctataag ttcctataat ggccaacttt aacttttcga taaaagtttt
     1561 ttgcagggaa agattatagc tgagcacacc cataaaacca ttgggcaggt tgttgtgatt
     1621 atagttgacg gaagttgagg cattgaactt ttgagggatt tggctgaact ggtacgacat
     1681 cacagcgttg taaattttac taccggaata cttttcggtt ctattctgct gctctgacgc
     1741 atcctggtag gtgaaagaaa cgataatatt ttgcctattt tcatctgtac tcctcagggt
     1801 gtaacttgca ttaccggacg ttgtcaggtt cagctgtgta aaactcaacg tatcgatgtt
     1861 ctggtaagga tttgtattgg tcaaatcatt atatatatcc cggatatgca cgtatgattt
     1921 taaattggag taggaggcac caacacttaa acgctttgtg attgaggccg aaacattgcc
     1981 agaaaagata aatcgattgt tggcattaac cttttgtttc gacaagttgt catgctgata
     2041 accggcatct atggcaatat tcacaatctt tctgattgtt gtggagtagt ttgccgttat
     2101 attctcaaaa tcgtttaaaa agtagtaagc actcagcgtg gtatagtttg ggctaatcct
     2161 ctcgtatgtc gcacccacag tgccaattgc tgatgtttgc gaaatacccg ctttaagtgc
     2221 atgatgaacc gacaggtcgc cattgctctc caggaagtta tccttaaaac tcctatcaac
     2281 gctatcgctc ctgctgatat cggcattaat tgcacttatg gcatactcca tattcaactg
     2341 aaagttgtta atcattttca tgtttaggta taagctaccc gaaatattat ccttaggtgt
     2401 taaagtgcta tcaatggtac tggtgtagaa atcgttgtta ttcacatcct ttgctttgta
     2461 gatatttagg gcaacatcga ctttctcatg gctatattcc accttaaaac caccccccat
     2521 tcgcctaaag gcggcggagt ttatggcgga atcgggtctc acctccttct gcaaccgacc
     2581 gtatatggcg ctaaacttaa aaccattatc gaacttgagc tctacaccac ccccaaacgt
     2641 ttcgtgtcca accaatgtgt aaggcgaaaa tgtcatactt gcgtagccag catacaatgt
     2701 aatccactta tacgaagggg caatggagaa acgattaaag ggtaacgtag cctcggtgtt
     2761 tagct
//
LOCUS       DAOSHM010000139         2701 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_36199_length_2701_cov_23.897581, whole genome shotgun
            sequence.
ACCESSION   DAOSHM010000139 DAOSHM010000000
VERSION     DAOSHM010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2701
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36199_length_2701_cov_23.897581"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            112..2682
                     /locus_tag="PK839_10715"
     CDS             112..2682
                     /locus_tag="PK839_10715"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG73248.1"
                     /translation="MNSRTKSNLILLLLLWTAVFFASMGHFTAYSQEKDSLIQLLSDA
                     KGKQKLEILFKLSELTSQTEEGIKFAEEALAVAKKMDRVSLIEAYENMAIAYSENFLD
                     EAALSYFKKAYQLSLEYGYIPGLLKACKGISTSYFYLDSINLSTKYSNELLAAAEKVD
                     SLNYEADAYFWLGRIKQAEGQTDSALMFIGKALKLREKLGNLKEVAQTYNGLGGLYFD
                     DAQYEKAIECYKKEIEIKEAIHDKPDYLALAYLNLGRAHIALSNFQLALEQYQKALRT
                     FESINNEKGIAVSNSGIGMIYENLSQSVMATEENEVNYTKALEYHKNALRLFRKMGQR
                     KEEGQTLQNIGNVFSRLATNRFVARFGEEWEDSLYKIKQDQILASFDSSLIYYQYALD
                     IFNQINDEREIVKVNTNLGSTYSWARDWPKATRYISQAVGICRRNGYQYELSAALYAQ
                     GESYYRQGNLAGAEASFLECVEISTRLGLKETLRYSYNRLSKLYEQKGDMAKALANLK
                     LSVRIKDEIFTEKSQKIITEMQTKYETEKKEQELKLMKNQDELQKSIIQRQKLMIIGA
                     IVGALLILMVALLMFKMFRDKQRANRILQEKNALITSQKKEITDSIQYASRIQSAVLP
                     TSSLLAEALPEHFVLFKPRDIVSGDFYWMTRKNEKVVLVAADCTGHGVPGAFMSMLGV
                     SFLYEIVNKENVLQPAEILNQLRIHIKTTLSQTGKQNEQKDGMDISLCVIDRPGNVLE
                     WAGAYNSLYQIRGGELTEYKADKMPVAVHVSDSNSFTNHQIEIQHGDTFYMFSDGYAD
                     QFGGTDGKKFMSKKFKELLVGIWNKPMDEQGKIINETHLQWKGEYDQVDDILVIGFRV
                     "
BASE COUNT          814 a          596 c          633 g          658 t
ORIGIN      
        1 aaacatcaaa caaacccaaa ttaagcaaat ttgtttacca gacatcttct ttttaaaagt
       61 tttgcatttt ggttaaactt ttgttaattt gccagttaaa gccttccccc gatgaacagc
      121 agaaccaaga gtaacctgat tctactactt ttactttgga cagcagtttt ctttgctagc
      181 atgggccatt ttactgcgta tagccaggaa aaggatagcc tgattcagct gctttcggac
      241 gctaaaggta agcaaaagct cgagattctc ttcaagcttt ctgagctaac ctcccaaacc
      301 gaggagggga taaaatttgc cgaggaagcg ctggctgttg ccaaaaaaat ggatagggtt
      361 tcgctaattg aagcctatga gaatatggcc attgcctact cggagaattt tcttgacgaa
      421 gcagcccttt cctacttcaa aaaagcctac cagctaagcc tcgaatatgg ctacatcccc
      481 ggcctgctaa aagcctgcaa gggtatttct acctcatact tttatctcga ttccattaac
      541 ctttctacta aatactcgaa tgaactttta gctgctgctg aaaaggttga cagcttgaac
      601 tatgaggcag atgcttactt ctggctgggt aggataaagc aggctgaagg gcaaaccgat
      661 tctgcgctta tgtttatcgg caaagcgcta aagttaaggg aaaagttggg caatttaaaa
      721 gaggtcgctc aaacctacaa cgggttgggc gggctttact ttgatgatgc gcagtatgaa
      781 aaggccatag aatgctataa aaaggagatt gaaatcaagg aggccattca cgataagccc
      841 gactacctgg cactggcata tttaaacctg ggacgtgcgc atattgcgtt aagcaacttt
      901 cagctggcac tggagcagta ccaaaaggca ctccgaactt tcgaaagcat caacaacgaa
      961 aagggcattg ccgtttccaa ttccggcatt ggcatgattt acgagaatct ctcgcaaagc
     1021 gtcatggcca ctgaggaaaa cgaagtgaac tacaccaagg cgctagagta ccataaaaat
     1081 gcgttgcgcc tgtttcgtaa aatggggcag cgaaaggagg aggggcaaac actccagaac
     1141 ataggcaatg ttttctcacg acttgcaacc aaccgttttg ttgcccggtt cggggaggaa
     1201 tgggaggatt cgctctacaa gattaagcaa gatcaaattc tcgcctcgtt cgatagctca
     1261 cttatttact accagtacgc acttgacatt ttcaatcaaa taaatgatga gcgggaaatt
     1321 gtaaaggtaa acaccaactt aggaagcact tacagctggg ccagggattg gccaaaagca
     1381 acccgataca tctcccaggc tgttggtatc tgccgtcgta atggctacca gtatgaactt
     1441 tcagctgcgc tttatgctca gggcgaaagc tactaccgcc agggcaatct ggccggggct
     1501 gaggcttcat ttctggagtg cgttgaaata tctaccaggt taggtctgaa agaaactctt
     1561 cgctacagct acaacaggct atcaaaactt tacgaacaaa agggagacat ggccaaggcg
     1621 ttggccaatc ttaaactttc ggttagaatt aaagacgaga ttttcacgga gaaaagccag
     1681 aaaatcataa ccgaaatgca aaccaagtac gaaactgaaa agaaggagca ggagttaaag
     1741 ctgatgaaaa accaggatga gctccaaaag tccatcattc aacgtcagaa gttgatgata
     1801 atcggcgcaa ttgtcggagc cctgctgatt cttatggttg ccctcctgat gtttaagatg
     1861 ttccgcgaca agcaaagggc caacaggata cttcaggaga agaatgcgtt gataaccagc
     1921 cagaaaaagg agattaccga tagcattcag tatgcaagcc gcatacaatc agccgttctt
     1981 cctacctctt cacttctggc cgaagccctg cctgagcatt ttgtactttt caagccaaga
     2041 gatattgtta gcggcgattt ctactggatg acccggaaaa atgaaaaggt tgttctggtt
     2101 gctgccgact gcaccggcca cggagtacca ggggctttta tgagtatgct tggggtttca
     2161 tttctctacg agatagttaa caaggagaat gtgctgcaac ctgcagaaat tctaaaccag
     2221 cttcgcatcc atatcaaaac aaccctttca caaaccggga agcagaacga gcaaaaagat
     2281 ggaatggaca tttccctttg tgtaatcgac cgcccgggca atgttttgga gtgggcaggt
     2341 gcctataatt ccctctacca aattcggggc ggagagctaa ccgagtacaa agccgacaag
     2401 atgcccgtgg ctgttcacgt aagcgatagc aactccttta ccaaccatca aatcgaaatt
     2461 cagcatggcg ataccttcta catgttttcc gatggctatg ctgaccagtt tggcggaaca
     2521 gatggtaaaa agtttatgtc gaaaaaattc aaggagctgc tggtaggaat ttggaataag
     2581 cccatggatg aacaaggcaa aattattaac gaaacccacc ttcagtggaa gggcgaatac
     2641 gatcaggttg acgacatcct ggtcataggg ttcagggttt gaaggtaacc ggtagagggc
     2701 a
//
LOCUS       DAOSHM010000140         2619 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_37902_length_2619_cov_18.859984, whole genome shotgun
            sequence.
ACCESSION   DAOSHM010000140 DAOSHM010000000
VERSION     DAOSHM010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2619
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37902_length_2619_cov_18.859984"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1930)
                     /locus_tag="PK839_10720"
     CDS             complement(<1..1930)
                     /locus_tag="PK839_10720"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOG73249.1"
                     /translation="MDLSVIIVSYNVKHFLEQCLLSVFNALDGIEAEVIVVDNASGDG
                     SCEMVRERFKPVKLIENTTNVGFSRANNQAIAIAKGEFILLLNPDTVLQEDTLVRCLS
                     FMRNHPDAGGLTVKMIDGKGKFLPESKRGFPSPVTAFFKIFGFASLFPRSKTFARYYL
                     GHLDKNSTHEIEVLPGAFMFLRAKTLREVGLLDEQFFMYGEDIDLSYRILKGGYKNYY
                     YPECQIIHYKGESTKKGSLNYVLVFYRAMILFTKKHVNSRGAGLLVGIINLAIYFRAF
                     LSLLHRLAKRLWLPLLDLALIIAGVALIVPWWEQQRFQMQHVYPREYAGFLLSLYVAV
                     WMLSLWLGGAYDKPRKVHAPSRSIAVGTVAILVIYSVLPAGMRFSRAIILITGAWSLA
                     TTQLVRYLMVWISPSSFATSLAAKRIAVLGNSNEVERVSKILNLTGISYKLVVSLTPD
                     KLTGDNGYLNHERLAELVRVNGVNEIIFCTQGISMGAIVDAMLYLSPQGVSYKIATRE
                     GSSIIGSNSIEAQGELYTLDFKALGSPSVKRAKRLFDLFSAAAVIITWPVNAFFIRKP
                     FRVMVEALSVLVGLKTWVGYGADSPEALSALPKIKPSVFPYIPVKYPEKNSTRELYLY
                     YARNYSVSLDLQILIRNLY"
BASE COUNT          763 a          615 c          592 g          649 t
ORIGIN      
        1 ggtataaatt tctaatcaaa atttgtaggt caagcgaaac gctgtagtta cgggcatagt
       61 agagatatag ctcccgggtg gagttttttt cagggtattt taccggaatg taggggaaaa
      121 ctgatggttt tattttaggt agggctgaca acgcttcagg tgagtctgca ccgtagccaa
      181 cccatgtctt taggccaacc agcacgctga gtgcttcgac catcaccctg aagggttttc
      241 gaatgaaaaa agcattcacc ggccaggtga tgataaccgc agcagcgctg aagaggtcga
      301 acagcctttt ggcacgctta accgatgggg atcccagcgc cttaaaatcg agggtgtata
      361 gctcgccctg ggcctcaatg gagttgctgc caataatgga gctgccctct cgggtggcaa
      421 ttttatagct cactccctgt gggctcaggt agagcatggc atctactata gcacccatgg
      481 aaataccctg cgtgcagaat atgatttcgt ttactccgtt taccctaact aattcggcca
      541 gccgttcatg gtttaggtat ccgttatccc ctgtaagctt atcgggggtt aaagagacca
      601 ccagcttata cgaaataccc gtcaaattca gaattttact aaccctttca acctcattag
      661 aattacccag cacggcgatc ctttttgcag ccaggctggt tgcaaagcta ctgggtgaaa
      721 tccaaaccat caggtagcgg acaagctggg tggtagcaag cgaccatgcg ccggtaatga
      781 gaataatagc ccgtgaaaag cgcatcccgg caggtagcac ggagtatatc accagaatgg
      841 ccacggtacc cacggcgatg cttcgggatg gggcgtgaac ttttcggggt ttatcgtagg
      901 cgccaccaag ccataacgac agcatccaaa cggcaacgta aagtgacagc agaaaacctg
      961 catactccct ggggtaaaca tgctgcatct ggaagcgctg ctgctcccac cagggaacaa
     1021 tcagagcaac tccggcaatt atcaacgcca gatcgagcag gggtagccac aaccgttttg
     1081 ccaacctgtg cagcagcgaa agaaaagctc tgaagtagat ggccaggttg ataatcccca
     1141 ccagcaaccc ggcaccccga gagttaacgt gctttttggt gaacagaatc attgccctgt
     1201 agaaaaccag cacgtagttg aggcttccct tcttggtact ttcgcccttg tagtgaatta
     1261 tctggcactc ggggtagtag tagttcttat atcctccctt tagaatcctg tatgacaggt
     1321 cgatatcctc gccgtacata aaaaattgct catcgagcag cccaacctct cggagtgttt
     1381 tagcccgtag gaacatgaac gcaccaggaa gtacctcaat ctcatgggtg gaatttttat
     1441 cgaggtgacc caggtagtac cgggcaaaag ttttcgaccg ggggaaaagc gaggcaaacc
     1501 caaatatctt gaaaaaggct gttacaggcg atggaaatcc ccgttttgac tcggggagga
     1561 attttccttt cccatctatc atcttaacgg ttaaccctcc cgcatcggga tggttccgca
     1621 tgaagctgag gcaccggaca agggtatcct cctgcagcac ggtatcgggg ttgagcagca
     1681 aaatgaactc cccttttgct atggcgatgg cctggttgtt ggcacgcgaa aatccaacgt
     1741 tggtggtatt ctcaatgagc tttaccggtt taaaacgctc acgaaccatt tcgcacgagc
     1801 catcgcccga ggcattatca accacaatca cttcggcttc gataccatca agggcattaa
     1861 aaacgctaag caggcactgc tccaggaagt gcttcacatt gtagctgact atgattaccg
     1921 agaggtccat aaatttatcc taataccgga ttaaccacct ttcaaacagc agtagtaaga
     1981 tggctgtaga tacaagcagc taaaaattca cggaataaaa gtaagatatt ttggctaacc
     2041 aatggcagta cctcgccaat gccctgtgta gccataattt accaaacatg tttcgcattc
     2101 aacaaaaccg cccaggagct acagggttgc aaatcaacac cagctaaaga taatttatac
     2161 ttcaatatca gcaatcaaac cctacaaaca ccctgcagga ttgtccattt accgaaccta
     2221 atgaaaatgt atgtagaacg ttcaactgtg aaatgttccg cagtaaccag tttgctttga
     2281 tcgaagattc tctatacaaa ataaacacta acacaaaaag taaaattatg atgcccctta
     2341 taaatagcgt acaactatat agaaaacttt tgaaatttca acatttgagc gtgctaacag
     2401 aatgtgtttg attaatggtt aagtatgcaa gggaaaaaag aatggtcata cgatacgcaa
     2461 ttatgtacat aaacttgact atctgtaatt taccaatttt acatttatat ttctatcata
     2521 agtcgcacca caaaagtttc cgttgggttg gtcttatggg gctgagctgt tcttacttcg
     2581 taaaaataaa acaaacaaag aagatgagta gtaataatg
//
LOCUS       DAOSHM010000141         2613 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_38067_length_2613_cov_12.898358, whole genome shotgun
            sequence.
ACCESSION   DAOSHM010000141 DAOSHM010000000
VERSION     DAOSHM010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2613
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38067_length_2613_cov_12.898358"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..623)
                     /locus_tag="PK839_10725"
     CDS             complement(<1..623)
                     /locus_tag="PK839_10725"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HOG73250.1"
                     /translation="MMDNEQLPKGYKNTEVGVIPEDWEVKAIGKYTDVTSGGTPSTLI
                     REYWDGPIRWMNSGELNLKIVYEVENRITELGLNESATKLVPKNCVLIGLAGQGKTRG
                     TVAINKVELCTNQSIAAILPSDEVFYEYLYYNLDSRYFELRGLSTGDGGRGGLNLTII
                     KNLLIPLPPTKAEQTAIATALSDADALINSLEKLIAKKRNIKQGAMQ"
     gene            complement(620..1483)
                     /locus_tag="PK839_10730"
     CDS             complement(620..1483)
                     /locus_tag="PK839_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007897916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HOG73251.1"
                     /translation="MEIVKQQHIESKIVMLRDVQVILDKDLASFYEVKPIRLREQVKR
                     NPNRFPTDFVFQLTENEVDSLVSQNAIPSKQSLGGYLPYVFTEQGVAAVAGVLKSEKA
                     AEVSITIARAFVAMRKFLFQNASVFQRLDRIELKQIQTDEKFERIFKALESGQATPDM
                     GIFFDGQVFDAYTFAVNLIKSATQSIVLIDNYIDESVLTLLSKRNIGVSASIYTKEIN
                     KAFQLDVKKHNAQYPSIEIKTFANSHDRFLIIDHKELYHIGASLKDLGKKWFAFSRMD
                     SLVNELLKRIK"
     gene            complement(1474..>2613)
                     /locus_tag="PK839_10735"
     CDS             complement(1474..>2613)
                     /locus_tag="PK839_10735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit M"
                     /protein_id="HOG73252.1"
                     /translation="EEISDPKNDYNLNIPRYIDSQETEDIQDIEAHLLGGIPNADIEA
                     LNEYWEVYPSLKAELFNASERENYSDLKIEKDQIKQTIFNHPEFTTFSKEMDEVFEAW
                     KTKNTQILKALVKGFKPKELVFRISEDILATYTGHALMNKYDVYQHLMNYWNDIMQDD
                     CYYLSVEGWKAELTITKQTKSVTEWDCDLVPKSLVIDRYFGVEAQYIATLEAEKETIA
                     AEISELEEEHNTEEGLFADVPDKVNKGNVQKRLKAIDTPKLKTENAEEIKVLKAYLKL
                     IDDQTAINKKIKEALAELDKKVIERYKTLTEDEIKQLVVDDKWMSVLERDVKTEMERI
                     SQRLTQRIKELAERYETTLPQQNAEVASLESKVTTHLQKIGFVWK"
BASE COUNT          646 a          543 c          488 g          936 t
ORIGIN      
        1 tgctgcattg ccccttgttt gatgttgcgt tttttggcaa tgagtttttc gaggctgttt
       61 atcagggcat cggcatcgct tagggcggtg gctatggcgg tttgttcggc tttggtgggg
      121 gggaggggga ttaataggtt cttaataata gttagattca atccacctct accgccgtct
      181 ccggttgata aacctcttaa ttcaaaatat cttgaatcta gattgtaata taagtactca
      241 tagaagactt catctgatgg tagaatcgca gctattgatt gatttgtgca taattcaact
      301 ttattaattg caacagttcc tcgtgtttta ccttgtccag ctaatccaat tagaacacaa
      361 tttttaggca ctaatttagt ggctgattca tttaacccta attcagtaat ccgattttca
      421 acttcataaa caatttttaa gtttaattca cctgagttca tccacctaat aggtccatcc
      481 cagtattctc gtataagtgt gcttggagtt ccaccagaag taacatcggt atattttcca
      541 atagctttaa cctcccaatc ctcaggaata acgcccacct cagtattttt atagcccttt
      601 ggcaattgtt cattatccat catttaatcc tttttaaaag ttcattaacc aaagaatcca
      661 ttcgcgaaaa ggcaaaccat tttttgccca aatctttgag cgaggcgcct atatggtaga
      721 gttccttgtg gtcgataatt aaaaacctgt cgtggctatt ggcgaatgtc ttaatctcaa
      781 tgcttggata ctgtgcattg tgctttttta catccaattg aaatgcttta ttaatctctt
      841 ttgtataaat gcttgcagat actcctatat ttcgcttgct caacagcgta agaaccgatt
      901 catcaatgta attatcaata agaacaatgc tttgggttgc tgattttatc aagttgacag
      961 caaaggtata ggcatcaaac acctgcccgt cgaagaaaat tcccatatca ggcgtggctt
     1021 gtccgctttc cagtgctttg aatattcttt caaatttctc atccgtttga atttgtttca
     1081 attcaatgcg gtcaagtcgt tgaaatacag aagcattttg aaataaaaac ttacgcatgg
     1141 ccacaaaagc ccttgcaatg gtaatgctca cctctgctgc tttttcgctt ttaagcacac
     1201 cggcaacagc agctaccccc tgttcggtaa atacatacgg aagatatccg cccaggcttt
     1261 gtttagaagg tatcgcattt tgcgatacca aactatcaac ctcgttttcc gttaattgaa
     1321 atacaaaatc tgtaggaaat cgatttgggt ttcttttaac ttgctccctt agccttatag
     1381 gttttacttc ataaaatgaa gctaaatctt tgtccaatat tacctgcaca tcacgtagca
     1441 tcactatttt tgactcaata tgctgctgct taactatttc catacaaacc ctattttttg
     1501 aaggtgagta gttactttgc tttcaaggct ggcaacttcg gcgttttgtt gtggcaaggt
     1561 tgtttcgtag cgttcggcaa gttcttttat acgttgggta aggcgttggc taatgcgttc
     1621 catttcagtc tttacatcac gctccaaaac gctcatccat ttgtcgtcta ccaccagttg
     1681 ctttatttcg tcctcggtaa gggtcttata gcgttcaatt acctttttat cgagttcggc
     1741 aagggcttct tttatttttt tgttaatagc ggtttgatca tcaatcagtt ttaaatatgc
     1801 tttcaatact ttaatttctt cggcattttc agttttcaat tttggtgtat caattgcttt
     1861 caatcgcttt tgcacattcc ctttgtttac cttatcggga acatcggcga ataacccttc
     1921 ttcggtatta tgttcttctt ccagttcgga aatttcagct gcaatggttt ctttttcggc
     1981 ttcgagagtc gcaatgtatt gcgcctctac gccgaaatag cggtcaatta ccaacgattt
     2041 tggcaccagg tcgcaatccc attcggttac cgatttggtt tgcttggtaa tggtcagttc
     2101 tgctttccag ccttcaaccg aaaggtagta gcaatcgtcc tgcatgatgt cgttccagta
     2161 gttcatcagg tgctggtaca catcgtattt gttcatcagg gcatggcctg tgtaggttgc
     2221 cagaatatct tccgaaattc taaaaacaag ttcttttggt ttaaagcctt ttacgagggc
     2281 tttcagaatc tgggtatttt tggttttcca tgcttcaaac acttcgtcca tttctttaga
     2341 gaaagtggta aattcgggat ggttgaaaat ggtttgtttt atctgatctt tttcaatttt
     2401 taaatcgctg taattttcac gctcacttgc attgaaaagt tccgctttca gtgaagggta
     2461 cacttcccaa tattcgttta acgcttcaat gtcggcattt ggtattccac ccaataaatg
     2521 agcttcaata tcctgaatat cttcggtttc ctgactgtca atatatcggg gaatgttcag
     2581 gttgtaatcg ttttttgggt cgcttatttc ttc
//
LOCUS       DAOSHM010000142         2576 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_38854_length_2576_cov_4.202697, whole genome shotgun sequence.
ACCESSION   DAOSHM010000142 DAOSHM010000000
VERSION     DAOSHM010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38854_length_2576_cov_4.202697"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(259..1173)
                     /gene="cysK"
                     /locus_tag="PK839_10740"
     CDS             complement(259..1173)
                     /gene="cysK"
                     /locus_tag="PK839_10740"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HOG73253.1"
                     /translation="MKFETAIESFGNTPHIRLSRLFPTHQVWIKDERRNPSGSIKDRA
                     AYAMVEEAERSGRLARGGVIVEPSSGNTGIGLAMIATVKGYRLILTMPETMSAERRKL
                     LKAFGAELVLTPASEGMQGAIEKAKAILSQIPDSWMPMQFDNHANADIHFRTTAAEIL
                     ADFPEGVDFLVAGVGTAGHATGCAARLKEIFPGLNLAVVEPESSPVISGGSAGDHFLQ
                     GIGAGFIPPNINLALIDRIVRVANDEAFAMVRRLAREEGMLVGISTGANLVAVEKIIA
                     GVEKSISILTFAYDSAERYLSIDDLWQE"
     gene            complement(1422..2198)
                     /locus_tag="PK839_10745"
     CDS             complement(1422..2198)
                     /locus_tag="PK839_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HOG73254.1"
                     /translation="MNTRYLKKKADLLVDCSLASLLPEHYPQHAARKHALRRPRVQIS
                     QAVALEGCFPSSRACEADQNFIETKFLTLRTTLLADAQFIFENDPAATSIDEVIVAYP
                     GFYAIACYRIAHSIYLLGARNVSRLITEIAHSRTGIDIHPGAEIGNEFFIDHGTGIVI
                     GETTVIGDRVKLFQGVTLGALSVSKPMRGMKRHPTVESDVVIYAGSTILGGETRIGHH
                     SVIGGNVWLVHSVPPNSLVYHESKVTVREREQLGFADGYS"
     gene            complement(2463..>2576)
                     /locus_tag="PK839_10750"
     CDS             complement(2463..>2576)
                     /locus_tag="PK839_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010956342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG73255.1"
                     /translation="LALVGESKGPGVADICEIIGKEETLQRIRQLVLVLGR"
BASE COUNT          693 a          634 c          508 g          741 t
ORIGIN      
        1 gggaggctgc tggcttaagc accttcgaca aagcggccaa acggcttgag agctctatcg
       61 tccccgaaat aattgaagta gtagttctgg gcagatcgtc ctactagcca attccataag
      121 gtttacttac aatccgattt gcttacctac ttttaccaat ggattaaatc atttaacagc
      181 ccgatcaatg attgtctgca tgcaataatc caaagagtat gcatgaaaaa cacagcatga
      241 acatgcggtt tacatccgct attcctgcca taggtcatct atactcagat acctctctgc
      301 actgtcgtag gcgaacgtta gtatgctaat agatttttca actcctgcaa taattttctc
      361 cactgcaacc agatttgccc cggttgaaat gccaaccagc atgccctctt ctcgcgctag
      421 tcggcgcacc atggcaaagg cttcatcatt cgctacccta acaatcctat caataagcgc
      481 taaattgatg ttgggtggta taaacccagc gccaattccc tgcagaaaat gatcgcccgc
      541 actcccaccc gaaataacgg gtgaggattc gggttcaacc accgccaaat tcaaaccagg
      601 aaaaatttcc tttagtcgag cggcacaacc agtagcatgg ccagctgttc caactccagc
      661 taccaaaaaa tcaacccctt cgggaaaatc ggctaatatt tctgcagcag ttgttcggaa
      721 gtgaatatct gcatttgcat ggttatcaaa ctgcatgggc atccatgaat caggtatttg
      781 actgagtatt gctttggcct tttctatagc cccctgcatt ccttccgaag caggggtaag
      841 cactaattca gcaccgaagg ctttcagtag ctttctgcgc tcggcagaca tggtttcggg
      901 catggtcagt attagcctgt atccttttac tgttgctatc atggccaggc caatgccggt
      961 atttccagat gaaggctcaa ctattacacc tcctctagcc aacctgccgc ttctttctgc
     1021 ttcttcgacc atcgcataag ccgctctgtc cttgatgctt ccgctagggt tacgcctttc
     1081 atcctttatc caaacctgat gggttggaaa taaccttgac aaccttatgt gcggggtatt
     1141 tccaaatgac tctatagctg tttcaaactt cattggtatt aattttactt gtttagttta
     1201 caaatatttg ctttgcagaa agactcccac ctcgccatgt ttaaaatgat acagggaact
     1261 tggagtagag cagtctgcac tttcaaagct gtagggcgtg tctattctcc atatcgtatc
     1321 gtttcacacc agctctacaa aaattaattc actgtaacct aagatttgag tacgccgtaa
     1381 ttcaactcaa attcctatag tctatggcag tcatgctgta ttcatgagta accatcggca
     1441 aaaccaagtt gctctctctc cctcactgtt accttgcttt cgtgatacac aagcgaattt
     1501 gggggtacgc tatgaaccaa ccacacatta ccgccaataa cgctatggtg tccaatcctg
     1561 gtttcgcctc ccagaatagt actgccggcg taaataacca catcgctctc gactgttgga
     1621 tgccgtttca tgcccctcat gggtttactt acgctcagcg ctccaagcgt aacaccttga
     1681 aatagcttta ccctatctcc aataacagta gtttcgccaa taacgatgcc tgttccatga
     1741 tcaataaaaa attcattccc aatttcggca cccggatgaa tgtcaatacc tgttctggag
     1801 tgggcaattt ctgtaattaa tcgggaaaca tttctggcac ccaaaaggta tattgaatga
     1861 gctattcggt agcatgcaat ggcataaaag cctgggtaag caacaatgac ctcgtcaatg
     1921 cttgtagctg ccgggtcatt ttcgaaaata aattgcgcat cagctagcag ggttgtccgc
     1981 agagttagaa attttgtttc gataaaattt tgatctgctt cacatgcccg tgagctggga
     2041 aaacatcctt caagggctac tgcttgggaa atttgaactc gtggtcgccg taaagcatgc
     2101 ttgcgagctg catgctgagg gtagtgctcc ggcagcaggc ttgccagtga gcaatctacc
     2161 aaaaggtctg ctttcttttt tagatacctt gtattcataa ctttattata aagtaaaaaa
     2221 accttccgtt tatgccggaa ggttcacata gtgctaaaat agctgctttg ctaataagta
     2281 cagtatggct ccctcgcggc tgttttccgt aagctacaac aaagccaaag ggtagcattt
     2341 tgtatcatta atggactgtg gtttattgaa atctattctc tattgaaaat cacactttag
     2401 ccaaagttga ggcgttcttt actgattttc taaaatacat gaaaccacaa atatattaaa
     2461 ttttatctcc caagaacgag taccagctgc ctaattcgct gtagggtttc ctctttgcca
     2521 ataatctcgc aaatatcggc aacgccaggg ccctttgatt cgccaacaag cgcaag
//
LOCUS       DAOSHM010000143         2470 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_41375_length_2470_cov_4.376812, whole genome shotgun sequence.
ACCESSION   DAOSHM010000143 DAOSHM010000000
VERSION     DAOSHM010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2470
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41375_length_2470_cov_4.376812"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="PK839_10755"
     CDS             <1..210
                     /locus_tag="PK839_10755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG73256.1"
                     /translation="NKSREIRNMLKMGHFNPEFSTQKMKESNPFNQMVLVDGLFTHID
                     TLPEDLQQMVREVRARGEDIEFFTN"
     gene            complement(925..1644)
                     /locus_tag="PK839_10760"
     CDS             complement(925..1644)
                     /locus_tag="PK839_10760"
                     /inference="COORDINATES: protein motif:HMM:NF024729.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HOG73257.1"
                     /translation="MIRDEIKGSRFHSEGYLKIARKLARKGHRIAKHRVNGLMRENSL
                     LSANRPVREGRKRKHDGKIATDAPNLMWATDGKKFFAGKDGWCWFFGVIEHFNDEILS
                     WHTAKKGNRFAAMEPVRAAVKQQFGSIEKGICSGMELKLRSDHGTQYDSADFMNEMSF
                     LGLGMSKAYVRSPECNGIIERFHRTLNEQVFSVSTFSCLEEANGAIAAFIENYNHHWV
                     LHRLKCHSPVEYRKMHETSAA"
     gene            complement(1815..2096)
                     /locus_tag="PK839_10765"
     CDS             complement(1815..2096)
                     /locus_tag="PK839_10765"
                     /inference="COORDINATES: protein motif:HMM:NF018350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOG73258.1"
                     /translation="MKKNNYWTSKRKAEVVLQLLRGEDIETLSRKYEITVSDLSQWRD
                     AFLSAGENGFKRAPEGSKLQKAERLIGQLQMELELTKKKNELMRRRSGS"
     gene            complement(2141..>2470)
                     /locus_tag="PK839_10770"
     CDS             complement(2141..>2470)
                     /locus_tag="PK839_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005803688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HOG73259.1"
                     /translation="FSNPVKHADTLSKIAIEYSRSDEEFAKAFKNKYSDPMPPSWMML
                     EVSSFGTLSSLYNYLHPSWDKREIAKHFGLADKVFSSWLHSIVYLRNICAHHARLWNR
                     EMQIQLY"
BASE COUNT          638 a          599 c          516 g          717 t
ORIGIN      
        1 aacaaatctc gagaaattag gaatatgctt aagatggggc attttaaccc tgagttttct
       61 acacaaaaaa tgaaagaatc aaatccattt aatcaaatgg ttttggttga tggccttttt
      121 acgcatattg atactttacc tgaagattta cagcaaatgg tcagagaagt gagagctaga
      181 ggagaagata ttgaattttt cacaaactag atataaaaac tgccatacgc cagaatacaa
      241 agcccagttc ggctcaggct ctgcctgagt tggattatcc ccacaggttg cacctgtaat
      301 gcaaactcct ttcccctcaa aagagttagc cacaacaaag aaacctgagt atatttttag
      361 taaggtttaa ttatgttgca tatataagtt tatggatagg tttactagcc aaattgtgaa
      421 aaatcccttg tggtacgtga caattcacca atccataatg ctaaaaggtt catgggaaag
      481 aatctgcgat ttttccaaag ggcttttaat aatttgggtt caaacagcgt agtattcaaa
      541 ccagcattta aacctaaact aaatgaatat tagccattta tgattatatt taaagaaaca
      601 atcctgaaaa ttctttaatt ctgggcataa tgattcagac attttgcata catttgcagc
      661 aacatgctta ccccgcttcc cgtaagaaca gcgcgctcag ggtaagcttt ttatttatac
      721 acatattgtt ttaaccctaa ataaccaagc atttttttta caattttaat atttattttt
      781 accagcggac aaacgaccga tggtgactgt tataccgaag gttcagggtg cgttagcaga
      841 ttgcttgcgc acccttttat ttttttcggg catcgagccc tcaaaaatta ggacaaacga
      901 cctcaggttc ctgttacccg atggctatgc tgcgctggtc tcatgcattt tacggtactc
      961 aacaggggaa tggcacttaa gccgatgcag cacccagtgg tgattgtagt tttcaatgaa
     1021 cgccgcaatg gccccattgg cctcctcaag gcaggagaac gtgcttactg agaaaacctg
     1081 ctcatttaaa gtcctgtgaa agcgctcgat gatcccgttg cactccgggc ttctcacgta
     1141 ggcttttgac attccgagcc ccaggaagct catctcattc atgaagtccg ctgagtcgta
     1201 ctgcgtaccg tggtcagacc tgagcttgag ctccatgccg gagcagatgc ccttttcaat
     1261 gctcccaaac tgctgcttta cggccgctcg aacgggctcc atcgccgcaa accgattccc
     1321 cttcttggcc gtatgccagc ttaatatctc gtcattaaaa tgctcaataa ccccaaagaa
     1381 ccagcaccag ccatcctttc ctgcaaagaa cttttttcca tccgtggccc acatcaggtt
     1441 tggcgcatca gtggctattt ttccatcgtg cttacgtttc cgtccttccc gaacaggccg
     1501 gttggcgctc agaaggctgt tctctcgcat tagcccgttc actcggtgct tggctattct
     1561 atgccctttc ctggccagct ttcgagcgat tttcaggtag ccctcgctgt gaaacctgct
     1621 cccctttatc tcatccctaa tcaactcaag cgcttggtca tccccaatag caggcctcgg
     1681 gccgggtcgc cgctttgctt cgccactagt cctagcgtac caagcgctgg agctgtagcc
     1741 aaccaccttg agtatgagcc tcagggggta gggcttttgg gttagggagc aaacctcggc
     1801 ggaaagcacc tcgactagct cccgcttcgt cttcgcatca gctcgttctt tttttttgtg
     1861 agctctagct ccatctgcag ctggcctata agccgctcag ccttttgcag ctttgagccc
     1921 tcaggggctc gcttaaaccc gttctcgcca gcgctcagaa aggcatcgcg ccattggctt
     1981 aggtcggata cagtaatttc gtacttacgg ctaagggtct caatgtcctc gcctcgcaat
     2041 agctgaagaa ctacttcagc ctttcgcttt gatgtccagt aattgttctt tttcataccg
     2101 tcagaaattt agcaatttgt ctgctcggtt ttaattgggg ctaatatagt tgtatttgca
     2161 tttcgcgatt ccacaaacgg gcatggtggg cacaaatatt tcgtaaatac actatgctgt
     2221 gcaaccacga tgaaaaaact ttatcggcta aaccaaaatg tttggcaatt tcccgtttat
     2281 cccaggaagg gtgcaaataa ttgtataggc tcgacaatgt accaaatgat gatacttcta
     2341 acatcatcca gctgggtggc atcgggtcgg aatacttgtt tttgaatgcc tttgcaaatt
     2401 cttcatcgct acgggaatac tctatggcta tctttgaaag agtgtcggcg tgtttaacag
     2461 gattagaaaa
//
LOCUS       DAOSHM010000144         2346 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_44663_length_2346_cov_20.026189, whole genome shotgun
            sequence.
ACCESSION   DAOSHM010000144 DAOSHM010000000
VERSION     DAOSHM010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44663_length_2346_cov_20.026189"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="PK839_10775"
     CDS             complement(<1..511)
                     /locus_tag="PK839_10775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG73260.1"
                     /translation="MRVRSCLFTALLVTLIGVGARAQAPNGISYQAVLRDADGKALAN
                     QAATLRLSLTSSDGTTVYFSETHSVQTDALGMMKVVLGGGQVETGSFAGVPWSSGGPI
                     YLKVEVKLDGQPSYTPMGSEQLQSVPYALHAANGQPMEWLGSLATPPENPTLNQAYYN
                     SVDKKSYVWD"
     gene            complement(558..1061)
                     /locus_tag="PK839_10780"
     CDS             complement(558..1061)
                     /locus_tag="PK839_10780"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOG73261.1"
                     /translation="MKHIFTTLILTLSMLAGYSQEVAMQVLATAGGYYENTGAGLSLS
                     WTLGEPAYTTLTSSEVILTQGFQQGNLFGTNLEEIPTDVTAIRVYPNPVTSTVNIAVT
                     LPNAEGRATVEVYDIAGRMVKSTSAVMVGHEPYRLDVSNLPSGIYLLRVATESPAAVR
                     VVKLVKE"
     gene            1219..1926
                     /locus_tag="PK839_10785"
     CDS             1219..1926
                     /locus_tag="PK839_10785"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HOG73262.1"
                     /translation="MMNDVIRLLNEQHSQSTPLEVLKWFLQEYQGRIALSSSLGAEDQ
                     VLTDMIVKIDPATRIFTLDTGRLFYETYELMEKTCLRYGISICVYFPEAGRVEKMVTE
                     KGINLFYESVENRKECCHVRKVEPLRRAFEGLDVWICGLRRSQSITRADGQLVEWDED
                     NGLIKLNPLIGWTEDEVWSYIRENHVPYNVLHDKGFPSIGCQPCTRAVMPGEDIRAGR
                     WWWENQDTRECGLHVKK"
     gene            complement(1955..2025)
                     /locus_tag="PK839_10790"
     tRNA            complement(1955..2025)
                     /locus_tag="PK839_10790"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1991..1993),aa:Cys,seq:gca)
     gene            complement(2082..>2346)
                     /gene="folB"
                     /locus_tag="PK839_10795"
     CDS             complement(2082..>2346)
                     /gene="folB"
                     /locus_tag="PK839_10795"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HOG73263.1"
                     /translation="VDVDTTNAQHTDRLDDTVNYQHIYNLVSVEMQVKSKLLEHVAER
                     IAAAIHASFPQVRSLKVKVSKMNPPLGGSLERVSVTLDRDFPL"
BASE COUNT          562 a          577 c          611 g          596 t
ORIGIN      
        1 catcccagac gtaggatttt ttatcaaccg agttgtagta ggcctggttt agcgtagggt
       61 tctccggtgg tgtagccaac gaacccagcc actccatcgg ttgcccgttg gccgcatgta
      121 gcgcgtaggg caccgactgc agctgctcgc tccccatcgg ggtgtaggag ggttgcccat
      181 cgagctttac ctctacttta aggtaaattg gcccgcccga cgaccaggga accccggcga
      241 agcttcccgt ctccacctgt ccgcctccaa gcacaacctt catcatcccc agcgcatcgg
      301 tctgcacgct gtgggtctcg ctaaagtaaa ccgtggtgcc atcgctgctt gtcaggctta
      361 gcctgagcgt agcagcctgg ttggccaacg cttttccgtc tgcatcccgc agcacggcct
      421 ggtagctgat gccgttaggc gcctgggctc ttgccccaac acctatcaga gtcaccagca
      481 atgctgtaaa aaggcaactc ctcactctca tggctattta tttttaaaag ttttttattc
      541 catttgccac atagctgcta ctccttcacc agcttcacca cccgcacggc agcgggagac
      601 tccgtggcca ccctcagcag gtagattccg gacgggaggt tggatacatc tagcctgtag
      661 ggttcatgtc cgaccatcac tgctgatgtg ctctttacca tgcggccagc aatgtcgtaa
      721 acttcaaccg tggctcgtcc ctcggcgttg ggcagcgtaa cggcaatgtt caccgtgctg
      781 gtcacagggt tggggtaaac ccgtatagct gtaacatccg tcggtatttc ctccaggtta
      841 gttccaaaga ggtttccctg ctgaaacccc tgggtaagga ttacctccga agaggttagc
      901 gtggtgtagg ccggctcgcc cagcgtccag cttagcgaca gacctgcacc cgtattctca
      961 tagtatccac ctgccgtggc cagcacctgc atggctacct cctgggagta cccggccagc
     1021 atggatagcg ttaaaatcag cgttgtgaag atgtgtttca tcctttttca ctttaaattg
     1081 tttagttgtt tgttgacttg taattagttt ttacacaagt taatacattt ttctaaatga
     1141 aagtcaagtt atttacataa aaatgatgag tatttttgac gagtggtata ttttgttaac
     1201 tatttcaatt tcagagtaat gatgaatgat gtaattcgat tgctaaacga gcagcatagc
     1261 caatcaaccc ctctggaagt gctgaaatgg tttcttcagg agtatcaggg acggatagcc
     1321 ctctcatcaa gccttggggc agaggatcag gtgcttaccg acatgatagt gaagattgac
     1381 cccgctacga ggatatttac ccttgacacg gggaggcttt tctacgagac ctacgagctg
     1441 atggagaaaa cctgcctaag gtatggaatt tcaatctgcg tttacttccc cgaggctggc
     1501 agggtagaga aaatggtaac ggagaaggga atcaacctgt tttacgaaag tgttgaaaac
     1561 cgtaaggagt gctgccatgt aaggaaggtt gaaccgttga gaagagcctt tgaggggctt
     1621 gatgtttgga tttgcgggtt aaggcgtagc cagtccatca cgagagctga tgggcagctg
     1681 gtggaatggg acgaggataa cggactaatt aagttaaacc cgctgattgg ctggactgaa
     1741 gatgaggtgt ggagttacat ccgagaaaac catgttccct acaacgtgct gcacgacaag
     1801 ggttttccga gcatagggtg ccagccatgt accagggcag taatgcccgg agaggatatc
     1861 agggcaggac gctggtggtg ggagaatcag gacacacggg agtgcgggct gcacgtaaag
     1921 aaataaaaaa gcccctgttg ggggcttata gatgaggtac cgagcggatt cgaaccgctg
     1981 taaatggttt tgcagaccag cgcctaaccc ctcggccacg gtaccatctt gctggtggca
     2041 aaaatataaa taaattacat tccggcaaaa ttcaacgctt cctacaacgg aaagtccctg
     2101 tcaagcgtaa cgctaaccct ttcaaggcta cctccaagcg gggggttcat ctttgaaacc
     2161 ttaaccttta agcttcttac ctgtgggaaa gaggcatgta tggctgctgc aattctttcg
     2221 gctacatgtt cgagcagctt tgactttacc tgcatttcga cgcttacaag gttgtaaata
     2281 tgctggtagt taaccgtgtc gtccaaccta tcggtatgct gggcattagt ggtatcaaca
     2341 tcaacg
//
LOCUS       DAOSHM010000145         2338 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_44947_length_2338_cov_5.223390, whole genome shotgun sequence.
ACCESSION   DAOSHM010000145 DAOSHM010000000
VERSION     DAOSHM010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44947_length_2338_cov_5.223390"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..542
                     /locus_tag="PK839_10800"
     CDS             <1..542
                     /locus_tag="PK839_10800"
                     /inference="COORDINATES: protein motif:HMM:NF013292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HOG73264.1"
                     /translation="VLLVGKGASEFARLHGATMVDSSYFYTKPRWDDLQRVLEHEKMG
                     TVGCVALDVNGNLAAATSTGGMTNKRWGRVGDAPIIGAGTYANNATCAISATGHGEFF
                     IRYTVAHDISALMEYKGLSLNDAASLVVSNKLVKVGGSGGVIGVDGQGNICMVMNTSG
                     MFRAFAKSDGQEGVAVFNK"
     gene            1018..1530
                     /locus_tag="PK839_10805"
     CDS             1018..1530
                     /locus_tag="PK839_10805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG73265.1"
                     /translation="MAINVETKKAAGSGLVKVFLLHFFIALLAAVFFLFVILLAAKEL
                     SWKVFFSYFITFIPITVVLPLILGIAAASSYKRVEIILTPASSVNLGKLKDFFLKNSY
                     LPAAEENSGFTFKRSHTLKRALCLNSDMPTIQVNDKAVSIVMLKRKSFTLVNLLSNDK
                     RYEVGSENAE"
     gene            1530..2132
                     /locus_tag="PK839_10810"
     CDS             1530..2132
                     /locus_tag="PK839_10810"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOG73266.1"
                     /translation="MISSPFLLLDSGRCKQNITRMAGKAQSSNLIFRPHFKTHQSHAV
                     GRWFREAGVSRITVSSAEMARYFARDGWKDITIAFPLSRFHVEAVNRLAEDIQLNITF
                     SSIQNLLDALPLITGNVGVFIEVDVGNNRSGVHVSNTRELALMVNQVAENPKLTFCGF
                     ITHAGQSYHVTGEKNVMAIHNQAMGELVKLKSFWSQQFRT"
BASE COUNT          644 a          445 c          547 g          702 t
ORIGIN      
        1 acgtattact tgttgggaag ggagcctcgg aatttgcccg cctgcatggt gcaacgatgg
       61 ttgatagcag ctatttttat actaaaccta ggtgggatga ccttcaacgt gtgctggagc
      121 atgaaaaaat gggaacagtg gggtgcgttg cacttgatgt aaatggaaat cttgctgcag
      181 caacttcaac aggaggaatg accaacaagc gatggggaag ggtaggagat gctccaatta
      241 ttggggctgg aacttatgct aataatgcaa cctgtgctat ttcggctacc ggccatggag
      301 aattttttat acgctatacc gtagcccatg atatttcagc attgatggag tataaggggc
      361 tgagtttgaa cgatgctgca tcactggttg ttagcaataa acttgttaaa gttggcggta
      421 gtggtggagt aataggtgtt gatggtcaag ggaatatatg tatggtgatg aacacttccg
      481 gcatgtttag ggcatttgcc aagtcggatg gtcaggaagg agtagctgtg tttaataagt
      541 aggttatatt ttgaagaaga acgattttct tactttagac ctaagctaca tgttcgataa
      601 ccgtttaaat tactattgat atttttttaa catcctctat ttcctggtta ctgaggaggc
      661 gataaaattc tttgcaacaa gttgaaggcg ctattaagcg tgggctatgg taagatttgt
      721 ttgaaaagtc gaaatgctaa aatttatccg gttaatgaat tggaattttt accttgccga
      781 ctgcttgcca ggtattaacc tacgtttaag tgttttttgt gatgcctccc aaatgaagga
      841 tggagaatat actttagaga atgtctcttt aacaattgat aattgaatga aatccccagg
      901 tggggctgct tgtataaaca cgcatgaaaa caaactgggt gttccatccg ctcaactccg
      961 atgcccaaag aatatgggcc aaagtattgt agtcagatac ctaaaaatac agatgttatg
     1021 gcgattaatg tggaaacaaa aaaagcagct ggttcaggtc ttgttaaagt tttcctactt
     1081 cactttttta ttgcgctgct agctgctgtt tttttccttt ttgttattct tttagcggct
     1141 aaagagctaa gctggaaagt tttttttagc tacttcatca cctttattcc tattacggtt
     1201 gtactcccgt taattcttgg aatagcagca gcctcttcat ataaacgggt tgaaatcatt
     1261 ctaactccgg cttcaagtgt aaatcttggt aaactaaagg attttttcct caagaatagc
     1321 tacctgcccg cagctgaaga aaattcaggg tttaccttta aacgctcgca tactcttaag
     1381 agagccctat gccttaatag cgatatgccg acaattcaag taaacgacaa ggcagttagc
     1441 attgtcatgc ttaaacgaaa atcgtttacc ctggtaaatc tgctaagtaa tgataagcgc
     1501 tatgaagttg gttcagaaaa tgcagaataa tgattagcag tccattcctt ttgctggata
     1561 gcggccgatg caagcaaaat atcacccgca tggcaggcaa ggctcagagt agcaatctga
     1621 tatttcggcc ccattttaaa acccatcaga gccacgccgt tggccgttgg tttcgcgagg
     1681 caggggttag cagaattacc gtttcatcgg ctgaaatggc ccgatatttt gccagggacg
     1741 gatggaaaga tattaccata gcttttcctc tatcccgttt ccatgttgaa gctgtaaatc
     1801 gtcttgctga ggatatacag ctgaatatca ccttctcatc aattcaaaat cttctggatg
     1861 ccctaccgct gattaccgga aatgttggag tattcattga ggttgatgtt ggtaataatc
     1921 gttcgggtgt ccacgtttca aatacgaggg aattagcctt aatggtgaat caagttgccg
     1981 aaaacccaaa gctaacgttt tgtggtttta tcacccatgc cggccagtcg taccatgtaa
     2041 ccggagagaa aaatgtcatg gctattcaca accaggcaat gggcgagctg gtgaagctaa
     2101 aatcgttctg gagccaacaa ttccgaacat aatcatatca tacggcgaca cgcccacctg
     2161 ttcgctggcc gatgatttct ggggtgtcaa tgaaattcgt cccggaaact tcgtttttta
     2221 cgatgttatg cagtatttga ttggctcatg tagcctggat gacattgctc tggccttaat
     2281 ttgcccggtg gttgatgttt atccagaaag agaggaggca gttgtccact gtggagcc
//
LOCUS       DAOSHM010000146         2283 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_46491_length_2283_cov_3.399013, whole genome shotgun sequence.
ACCESSION   DAOSHM010000146 DAOSHM010000000
VERSION     DAOSHM010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46491_length_2283_cov_3.399013"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..811)
                     /locus_tag="PK839_10815"
     CDS             complement(<1..811)
                     /locus_tag="PK839_10815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG73267.1"
                     /translation="MRKLLVLVFLLFALINSGLAQTGYPIFVTPTLTPPYSLKLSEYS
                     KFGSQRMVVNIAVNDLNISNLPVKLHIKLETVGVTIETPVTINTTPIYLDGGAVTILF
                     GDDLTDYFNINNLIFKGYSKEAYRATGQLPEGFYRVTVEVLHYQTNRLISNSGSVSAW
                     MAMGKPPVLKLPGNNQVMGEFPGMPLTFSWLPCNLGNPVSAGSVQYKFEMWELRVNGV
                     SPYVVASTVPVFYEETTQNTLLSVYPASLPMETGMQYAWRVTASDMNGLVPF"
     gene            complement(913..>2283)
                     /locus_tag="PK839_10820"
     CDS             complement(913..>2283)
                     /locus_tag="PK839_10820"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOG73268.1"
                     /translation="YGNPIGSQDSVGQLVQNGYYTLNVRDKNGCPQKLQQLINPSTNP
                     KVTRVGTTDVVCYGESNGTATIQDYQYGSPSAPLTFKWSNGNTGLSASGYPSGIHHVT
                     VTDTNSCSTAKFFEVVQPDLLRIAIVNSKMPKCFGYSDAFIEALGVGGVGGYSYLWST
                     GDSLSLLGNIGKGEYGLLLTDANGCEVYGVYPVGEPEELEVDLGDDILMCPGNTRVLD
                     GQEFATHKWSTSQGEVSKERYFTVSKPDSYFLEVTDSIGCLAHDTVAVAIGNSALVAD
                     FLVSSGVVLGDTLILYELSNMPLDSMSWDYNNSAFLELDSEMALDYILYLKTLSKGLY
                     NIDLYAYSGGCVSVATKQVEIVEDLGENPDDDGLGNDPLIREFTVNPNPNTGDFTVTV
                     KLREVADINLVLFSVASGLKVNEQFEYQLQDYLVDYNLVNLNSGVYLLIVKAGNERRQ
                     VKIVIQ"
BASE COUNT          571 a          588 c          540 g          584 t
ORIGIN      
        1 cgaagggcac aaggccgttc atgtccgagg cggtgacccg ccaggcgtat tgcattcccg
       61 tctccatggg cagggaggca gggtaaaccg acaggagcgt gttctgcgtt gtctcctcgt
      121 agaacacggg aacggtcgag gccaccacgt aggggcttac gccattgaca cgcagctccc
      181 acatctcaaa cttatactgt acactacccg ccgaaactgg atttcccaag ttgcagggta
      241 gccacgagaa tgtaaggggc attcccggaa actcgcccat cacctggttg ttcccgggca
      301 gctttagcac ggggggcttg cccatggcca tccaggcact cacgcttccg gagttcgata
      361 tcaacctgtt ggtctggtag tgcagcacct ccacggtaac ccggtagaac ccctcgggta
      421 gctgcccggt tgcgcggtat gcttccttgc tgtagccctt aaatataagg ttgttgatat
      481 taaaatagtc ggttaaatcg tcgccaaaaa gaatggtaac tgcaccacca tctaaatata
      541 ttggggttgt attaatggtt actggagttt caatggtaac acctacagtc tccagtttta
      601 tatgcaactt aacgggaagg tttgagatat tcaaatcgtt caccgcaata ttaaccacca
      661 tgcgttgtga gccaaacttg ctgtactcgc tcaactttaa ggagtaaggg ggggttaaag
      721 ttggggttac gaatattggg taaccagtct gagctaatcc gctattaata agtgcaaaga
      781 gtaaaaagac taaaactaat aatttacgca tgctcctttt tattgtgtag ttgcattata
      841 acaacctgtt aatactttat ttaacttcta acacccctgt cagggtttaa aaccctgaca
      901 ggggtttatt acctattgga ttacaatctt aacctgccta cgctcgttgc ccgctttaac
      961 aattagcagg taaacccctg agttcaggtt taccaggttg taatcgacta agtaatcctg
     1021 caattggtac tcgaactgct cgttcacctt cagccccgat gccacggaga atagcacaag
     1081 gttgatatcg gcaacctcac gtagcttcac cgtaacggta aagtcgcccg tgtttgggtt
     1141 tgggttaacc gtaaactccc tgataagggg gtcgttaccc aggccatcat cgtcggggtt
     1201 ttcacccaaa tcttcaacta tttccacctg tttggtggca acggaaacac agcctcccga
     1261 gtaggcgtag aggtcgatgt tgtataggcc cttgctcagg gttttgaggt agaggatgta
     1321 gtccaacgcc atttcgctat ctagctccag gaatgccgag ttgttgtagt cccagctcat
     1381 gctatcgagc ggcatgttgg acagctcgta cagtatcagg gtatcgccca ggacaacccc
     1441 tgacgaaacc agaaaatcgg ccaccagggc gctattgcct atggcaacag caactgtatc
     1501 gtgcgccaaa catccaatac tatcggtaac ctcaaggaaa tagctatcgg gttttgaaac
     1561 tgtaaagtag cgctctttgg acacctcacc ctgcgatgtg ctccatttgt gagtagcaaa
     1621 ttcctgccca tcgagtacac gggtattccc agggcacatc aggatgtcgt cgcccagatc
     1681 cacctccaac tcttcgggtt cacccaccgg gtaaacacca tacacctcgc agccattggc
     1741 atcggttaat agtaatccat actcgccttt cccgatatta cccagcaagc taaggctatc
     1801 gccggttgac cataggtagc tgtaaccacc aacaccacca acgcctaatg cctctataaa
     1861 ggcatcgctg tagccaaagc actttggcat tttactattg acaatggcaa tcctcagcaa
     1921 atcgggctga accacctcga agaacttagc ggtggagcag ctattggtat cggtaaccgt
     1981 aacgtggtga attccagatg gatagccgct agccgacaga ccggtattcc cattggacca
     2041 tttaaatgtt agtggagcgc tcggactgcc gtactggtaa tcctgtattg tagcagtacc
     2101 gttgctttcg ccatagcaaa caacatcggt agtgccaacc ctggttactt ttgggttggt
     2161 tgatggattt atcagctgtt gcaacttttg cgggcagcca ttcttatcgc gcacatttag
     2221 cgtgtagtag ccattctgca ccagctgtcc aacggaatcc tgactgccaa tgggatttcc
     2281 gta
//
LOCUS       DAOSHM010000147         2272 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_46788_length_2272_cov_12.332431, whole genome shotgun
            sequence.
ACCESSION   DAOSHM010000147 DAOSHM010000000
VERSION     DAOSHM010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46788_length_2272_cov_12.332431"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(303..>2272)
                     /locus_tag="PK839_10825"
     CDS             complement(303..>2272)
                     /locus_tag="PK839_10825"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOG73269.1"
                     /translation="FVFLFIQVVIGIAQTPTPLLWPIKNAAPGERIIGKPGDTIAGQL
                     NYDELYIGATDSDVVVAPIDGIITDVGYWHSFPLNFSKLKGKVFPRSDTIRDDNGDKA
                     YRNSWAAIIKEQNPQLNMDIANYISYSIGIKVGTDEMYYISGLRPVRYFKTGEAVKRG
                     DVIGTVGYCDTAFNVPHIQISRSIKTKSEDPMGVFGIPSTFKKYKPKINYITEKHPAS
                     NLVEAFSIMRTGLEQAHPGLYDYTPKGKMDSLFMVTQQKINKPMTSEEFRRLITPIVT
                     AIRDVHTAIYPEAYSNKAATTPEVSIGVVNGVLQFIQPCKGKQINTGTPIVAINGQPS
                     GKIIQFLRTQYYRKDGYNELPVESELLSNFWSMYFKAYTPSASTPLKLTLENGQVFSF
                     KYLPINATTINDDALSMSIQMIKPNIGYIDINSFNLNQVEEDSILHFIQSLEKDKLNN
                     LIIDVRNNKGGNLDVLYRLTSYFISKSYRRNCYREVKSNTTYPAFRFTDNYSADDVLF
                     GDFHSITSKNAFRKEDTLMVVPSSTHYSGRLYILVDEFSVSAASEFAAVFAEQGIGTI
                     IGRETGTCYYQMNAEKFAYFHLGNTGLELMVPLIKIVAREALHPRIPYGHGVIPDIEV
                     PLTLDEITLQKDIILERALENIVVQKSVSKR"
BASE COUNT          685 a          462 c          448 g          677 t
ORIGIN      
        1 accaggcaaa tcccgaatga atagattagt ctgaggcaca caacctcaac taaactccgc
       61 taccgcacga accgaccgta gggagctcaa cgaagttagt cctctcgtgt ggtagtatag
      121 aaaaggaaag taatgaatat gaattagtaa aaagataatc cagcgggatt atgcgttata
      181 aacttaagga ctggagtagt aagcgttgtt gttgagaggt agggatgaga ttgcctcacg
      241 actgtagtcg tgaggcattg gaggaaatta caactatggc tcatccataa aaaagaagaa
      301 ttttatctct tagatacact tttctgcact acaatatttt ccaaagcacg ctctaaaata
      361 atatcctttt gaagcgtaat ctcatctagt gtcagaggaa cctctatgtc tggaataact
      421 ccatggccat agggtattct agggtgaaga gcctcacgag caacaatctt aattagcgga
      481 accataagtt ctaagccagt attgcctaaa tgaaaatagg caaacttctc tgcattcatc
      541 tgatagtaac aagtacccgt ttccctacca ataatagttc caatgccttg ttctgcaaat
      601 accgctgcaa attccgaggc ggcggatacc gaaaactcgt caacaaggat atataacctg
      661 ccactatagt gagtgctcga tggcacaacc attaaggtat cttctttcct aaaggcgttt
      721 ttactagtaa tggaatgaaa gtcaccaaaa agaacatcat ctgcactgta gttatctgta
      781 aagcgaaatg caggataggt ggtattactt tttacctccc tgtagcaatt tctgcgatat
      841 gacttgctaa taaagtaaga tgttaaccgg taaagcacat ccaggttacc tcccttatta
      901 tttctaacat cgataattag attatttaac ttgtctttct ccaaggactg aatgaaatgc
      961 aaaatagagt cctcctcgac ctgattcaaa ttgaacgaat tgatatcgat gtaacctata
     1021 ttaggtttta tcatctgaat cgacatgctt aaagcatcat cattaattgt tgttgcattg
     1081 attggaagat atttaaaact aaacacctga ccgttttcaa gggtaagttt aagaggagtt
     1141 gatgcggagg gagtatatgc cttgaaatac atgctccaga aattggataa aagttcggac
     1201 tctactggca gctcgttata gccatcctta cggtagtatt gtgttcgtaa aaattgaatt
     1261 atcttaccag atggttgccc atttattgca acaattggcg tacctgtatt aatttgtttt
     1321 cccttacaag gttgaataaa ctgcaataca ccgtttacaa cacctattga tacttcggga
     1381 gtagtggctg ccttgttgga ataagcctcg ggataaatgg ccgtatgtac atctcggatg
     1441 gcggttacaa tgggagtaat cagccttcgg aactcctccg atgtcattgg tttatttatc
     1501 ttttgctggg taaccataaa taaactatcc atttttccct tcggggtgta atcatatagc
     1561 cccggatgcg cctgctccaa ccctgtgcgc attatactga atgcctcaac caggttgctt
     1621 gcagggtgtt tctcggtaat gtaattaatc ttaggcttat actttttgaa tgttgatggt
     1681 atcccaaaaa cacccatggg atcctccgat ttggttttaa tactcctaga aatctgaata
     1741 tgcggaacgt tgaaagctgt atcgcaatat ccaacagtgc caataacatc accacgcttt
     1801 acggcttccc ctgttttgaa ataacgcact ggccgaagcc ctgaaatata gtacatctca
     1861 tcggttccga cttttattcc gattgaataa gaaatgtagt tggcaatatc catgtttaac
     1921 tgggggtttt gctctttgat aatagcagcc caagaatttc ggtaagcttt atccccatta
     1981 tcatcacgaa tcgtatcgga tcttggaaat actttacctt taagtttcga aaaattcaac
     2041 ggaaatgaat gccagtaacc aacatctgta ataatcccat ctattggggc aactactaca
     2101 tcgctatccg ttgccccaat gtatagctca tcataattta attgacctgc aattgtatcg
     2161 ccaggttttc cgatgattcg ctcaccagga gcagcatttt tgatgggcca gagaagtgga
     2221 gtgggtgttt gtgcaattcc tattactact tgaataaata gaaaaacaaa aa
//
LOCUS       DAOSHM010000148         2265 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_46988_length_2265_cov_20.476923, whole genome shotgun
            sequence.
ACCESSION   DAOSHM010000148 DAOSHM010000000
VERSION     DAOSHM010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46988_length_2265_cov_20.476923"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1479
                     /locus_tag="PK839_10830"
     CDS             <1..1479
                     /locus_tag="PK839_10830"
                     /inference="COORDINATES: protein motif:HMM:NF013779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C10 family peptidase"
                     /protein_id="HOG73270.1"
                     /translation="YKPEGWAIVAADDRIPAIIAYSLKGSFDTVKIKEVPFYFWFQHY
                     ANQIKEVVEGKETKRHASWDNHPFEAQKSTSPVEPLIGVEWNQSSGWNAYCPADSEGP
                     GGHAYAGCVAVAMGQALSVYQHPEVGYGQNYYVDDTYGSLGAVFGDTQYKWDSIMGLD
                     QPNKYVALLLYHLGVSVDMNYGADGSGAFSSMVPGALKTFFDYTNSTTILKRSNFTSA
                     QWDSILVSELANGRPIYYSGDPGDGTAGHAFNIDGVDDQNRYHLNWGWSGAYNGYYLI
                     TSLTPGSNNFSSNQEAVVNIMPRNHNPQDILLSNNSIMEGLPAYSLVGKVNVLDETPG
                     DSHTLEVIAPPTIDGEPGFVPFAILGDSLLTTEILDFQTRNRYEIIIKATDKAGHSLE
                     KSFFINVIKNTGVSDLTLSGEMIITPTNDGNLKVWLNNSAIGRATMNLYDINGKLIFS
                     TTISKELQVLDTTIPLGNTLIKGLYIAVLNFGNEKISYKFVK"
     gene            1526..2194
                     /locus_tag="PK839_10835"
     CDS             1526..2194
                     /locus_tag="PK839_10835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG73271.1"
                     /translation="MVKSIVQIFFLMGMALVGISQECTTMWPYIYPEFKEGTLLMVDG
                     SKLTAQFNVHVQEGRLHYLVKGIIKEARSGDILLANIGDDIYMNVDGSVMKVVGSEER
                     GFVATLILADFDKLLNSTQGAYGASLNSSAKMKLSSIEVGGKTIVDHMLLKQRKEEGE
                     TLPLMYEYFIVTRGKVFPATKKSIMSQLDDKESAEFKKFLKVNKIKWNDPQSLLKLLE
                     FFNR"
BASE COUNT          668 a          411 c          521 g          665 t
ORIGIN      
        1 tacaaaccgg agggttgggc aattgttgca gccgacgata ggattccggc cataattgca
       61 tactcgctta agggtagctt tgacaccgtt aaaattaagg aagttccatt ttacttctgg
      121 tttcagcatt acgccaacca gataaaggag gttgtggagg gaaaagaaac caaaagacat
      181 gcttcatggg ataaccaccc ctttgaggca caaaaaagca cttcacccgt tgagccgctt
      241 ataggggttg agtggaacca gagttcaggg tggaacgcat actgtccggc tgatagcgag
      301 ggacctggtg gtcatgccta cgcgggttgc gtggctgtag ccatgggtca ggcattgtcg
      361 gtttaccagc accctgaagt gggatatgga cagaactact atgtagatga tacctacggg
      421 agccttggtg ctgtttttgg cgatacgcag tataagtggg actccatcat gggtttagat
      481 caaccaaata agtatgtggc tcttcttctc taccatcttg gggtatcggt tgatatgaac
      541 tatggcgctg atggctcagg agccttttcc agcatggtac ctggcgcttt aaaaacgttt
      601 ttcgactaca ccaacagcac taccatatta aaacgcagca actttacatc cgcccaatgg
      661 gattctatcc tagtaagcga gcttgcgaat ggacgaccta tttactactc cggcgatccc
      721 ggcgatggca cagctggtca tgcatttaat attgatggag ttgatgacca gaatcggtac
      781 catttgaact ggggctggag cggtgcatac aacggttact acctgattac ctccttaact
      841 ccgggttcaa ataattttag cagcaaccag gaggctgtag tgaatattat gccacgcaat
      901 cataaccctc aggatatatt actatccaac aacagcatta tggagggatt acctgcatat
      961 agtctggttg gtaaagttaa tgtgctggat gaaacacctg gcgattctca taccctggag
     1021 gtaattgccc ctcctactat tgatggcgag cctgggtttg tcccctttgc tattttagga
     1081 gatagcttat tgacaactga aattttagat tttcaaacga gaaaccgcta cgaaattatt
     1141 attaaggcta ccgacaaggc tggtcattcg ttggaaaaat cattttttat taacgtgatt
     1201 aaaaatacag gtgttagcga cctaacttta agtggcgaaa tgattattac acctacaaat
     1261 gatggcaact taaaagtgtg gcttaacaac tcagctatag gtagggcaac catgaattta
     1321 tacgacatta acggtaaact catattttct actacgatta gtaaggaatt acaagtgctg
     1381 gataccacca ttccactagg taacactcta ataaagggac tttatatcgc agttttgaat
     1441 tttggtaatg aaaaaatttc ctataaattt gtaaagtagg ctaagggtca aggtatgaaa
     1501 tttaaaattt taaactcgtt acagcatggt taagagtatt gtacaaatat tttttttgat
     1561 gggtatggca ttggtaggta tttcgcagga atgtacaacc atgtggccat acatctatcc
     1621 tgaatttaaa gagggaactt tacttatggt tgatggtagt aagctaacag cccagttcaa
     1681 cgtgcacgtt caggagggtc gtttacacta tctggttaaa gggattataa aagaagctag
     1741 gagcggggat attttgctgg ccaatattgg agatgatatt tacatgaatg ttgacggcag
     1801 tgttatgaag gtggttggga gcgaggaacg gggatttgtg gcaaccttaa tacttgctga
     1861 ttttgataag cttttaaatt ctacccaggg tgcatatggt gcttccttaa actcttctgc
     1921 taaaatgaag ctttcttcca tagaggtggg cggtaaaact attgttgatc acatgctgct
     1981 aaagcagaga aaagaggaag gtgaaacctt gccattaatg tatgaatact ttattgttac
     2041 aagggggaaa gtttttcctg ctacaaaaaa aagcattatg tcgcaactgg acgacaaaga
     2101 atcggctgag tttaagaaat ttttgaaggt taacaaaatt aaatggaatg atccacaaag
     2161 tcttttaaaa ctattagaat tttttaaccg ttaacaattt aatattaagc atttaagggt
     2221 taagggtttc tttaaccctt ttttattttg tgactttttg aatgc
//
LOCUS       DAOSHM010000149         2222 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_48356_length_2222_cov_13.931703, whole genome shotgun
            sequence.
ACCESSION   DAOSHM010000149 DAOSHM010000000
VERSION     DAOSHM010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48356_length_2222_cov_13.931703"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(408..671)
                     /gene="cas2"
                     /locus_tag="PK839_10840"
     CDS             complement(408..671)
                     /gene="cas2"
                     /locus_tag="PK839_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018964772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HOG73272.1"
                     /translation="MYVVLVYDVGEKRVGKMLKLCRKYLNWIQNSVFEGEITDVKLNE
                     LKWKAIQLLDEETDSIIIFKSRQEKWLDKEIVGRERQSTSNFI"
     gene            complement(707..1711)
                     /gene="cas1b"
                     /locus_tag="PK839_10845"
     CDS             complement(707..1711)
                     /gene="cas1b"
                     /locus_tag="PK839_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004373514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated endonuclease Cas1b"
                     /protein_id="HOG73273.1"
                     /translation="MKKNYYLFNPGRMSRKDNTLKFTPVDESGSELAPRYIPVEQVDS
                     IYAFGSLDANSALFNFLGRMNIAVHFFDYYENYTGSFMPRDELLAGRMLIAQTQHYLD
                     MQKRMAIARQFIDGASFNMLKNLKYYTTRGKDLTPHIEAIETLRSGIANALTVEELMG
                     VEGNIRQDYYQTFDTILNDYEMGPRTKRPPTNMVNALISFGNMMCYSECLRAIHQTQL
                     NPTISYLHQPGERRYSLALDIAEIFKPILVDRVIFKVLNKREIQENDFEEKLNYVVLK
                     ERGKKRFIEEYEQRMQETIKHRTLNRNVSYKHLVKLECYKLQKHLLDIEMYKPLKMYW
                     "
     gene            complement(1821..>2222)
                     /gene="cas4"
                     /locus_tag="PK839_10850"
     CDS             complement(1821..>2222)
                     /gene="cas4"
                     /locus_tag="PK839_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018358700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HOG73274.1"
                     /translation="GKLIHETSYPQRSERFQEIEIEGIRLDYYDPVHRVVHEIKKSDK
                     RENAHIEQLKYYLYILEKNGIVGVTGILEYPKLHHTQPVELTQNDRETLEQHLTTIHN
                     IIEQDTCPPPLPITKCTSCSYYDFCWSGEEE"
BASE COUNT          478 a          492 c          525 g          727 t
ORIGIN      
        1 tcgtttgatt tccaacgttt tgttttcaat tccactatgg ttcggttaaa aggttgaagc
       61 tcgatattgg tttacaaata ttaaagaaat ttcaattcca ctatggttcg gttaaaaggc
      121 aaaaaccgtt gaggaagcgc tggtctggta gtaaatttca attccactat ggttcggtta
      181 aaagccgttg cggtggggaa aacgatcccc ctgtttctgg actcttgctg ttgtaaagct
      241 actacttttt tcccattttt gttgtcgtac cccaatagta ctttttccct ggctgttcga
      301 caatcgcttt taagctgcta taattcagct agtagcgaat atttgagcat ttttttgcct
      361 ttctcgactg ccattttttt tattgcagct gggtgttcga caactggcta gatgaagtta
      421 ctggtgctct gccgctccct gcctacaatt tccttatcaa gccatttctc ctgcctgctc
      481 ttaaaaatta taatgctgtc ggtttcctcg tcaagtagct gtatggcctt ccacttcagc
      541 tcgttaagct tcacatcggt tatttcacct tcgaacacgg agttttgaat ccagtttaga
      601 tacttacggc atagcttcag cattttgcct acgcgctttt ccccaacgtc atacaccaga
      661 acaacataca tggtttggtt ttttgtttta aaccagcagg ttttacctac cagtacattt
      721 tcaggggttt gtacatttca atgtcgagca ggtgcttctg cagcttgtag cattccagct
      781 taaccaggtg cttgtagctg acgttcctat tcagggtacg gtgctttatg gtttcctgca
      841 tgcgctgctc gtactcctcg ataaaccttt ttttaccccg ctccttcaac actacgtagt
      901 ttagcttctc ctcaaagtca ttttcttgta tctccctttt gttcaacact ttaaaaatta
      961 ccctgtccac caggatgggc ttaaagattt ctgcaatatc gagtgccagg ctgtaccgcc
     1021 gctctccggg ctggtgtagg tagctgatgg tggggttgag ctgggtttgg tgaatggctc
     1081 gcagacattc cgagtagcac atcatgttac caaacgagat aagcgcattc accatgtttg
     1141 tgggggggcg tttagttcgt gggcccatct cgtagtcgtt gagaatggta tcgaaggttt
     1201 ggtagtaatc ctgccggatg ttcccctcaa cgcccataag ctcttcaacg gttagggcgt
     1261 tggctatgcc cgatctcagc gtttcaatgg cttcgatatg gggtgtaagg tcttttccgc
     1321 gggtggtgta gtactttagg ttcttaagca tgttgaagga tgcgccatct ataaattgac
     1381 gggctatggc catacgcttc tgcatgtcga ggtagtgctg ggtttgtgca atgagcatgc
     1441 gaccggccag cagctcgtcg cggggcatga acgagccggt gtagttctcg tagtagtcga
     1501 aaaagtgtac ggcaatgttc atcctgccaa gaaagttgaa aagggcggag ttggcatcga
     1561 gcgagccgaa tgcgtatata gagtcaacct gctccacggg tatgtacctt ggggccagct
     1621 cgctgcccga ctcgtcaacg ggtgtgaatt tcagggtgtt gtccttgcgg gacatccgcc
     1681 cggggttgaa gaggtagtag ttttttttca caacttgcct aggttggttt tgcctttgct
     1741 gatgttgaaa ttattgttgt attacgccaa ttattaattt acaaactagt tttcttaaaa
     1801 gctaattgct gcctttccag ctactcctcc tccccactcc agcaaaaatc atagtatgag
     1861 cagctggtgc attttgtaat aggcaaaggg ggagggcagg tatcctgctc tataatgtta
     1921 tgtatggtgg taaggtgctg ctccagggtt tccctatcat tttgagtaag ttcaaccggt
     1981 tgggtgtggt gaagtttcgg gtattcaagt attcccgtta cgcctacaat tccattcttt
     2041 tctaaaatgt aaaggtagta cttgagctgt tcgatgtggg cattttccct tttatccgat
     2101 ttttttattt cgtgcacgac tctgtgcaca gggtcgtagt agtctagcct tataccttca
     2161 atttcaattt cctgaaatcg ctcggaacgc tgcgggtacg atgtttcgtg aataagcttg
     2221 cc
//
LOCUS       DAOSHM010000150         2071 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_53686_length_2071_cov_19.786706, whole genome shotgun
            sequence.
ACCESSION   DAOSHM010000150 DAOSHM010000000
VERSION     DAOSHM010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53686_length_2071_cov_19.786706"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(85..414)
                     /gene="trxA"
                     /locus_tag="PK839_10855"
                     /pseudo
     CDS             complement(85..414)
                     /gene="trxA"
                     /locus_tag="PK839_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008139937.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
     gene            660..>2071
                     /locus_tag="PK839_10860"
     CDS             660..>2071
                     /locus_tag="PK839_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOG73275.1"
                     /translation="MEEMQQYLKHKVFSVVSRVAEVEEVRVYVIGGYVRDIFLNRTSK
                     DIDIVVVGSGIDFAQRVARLMPGARMAYFKNFGTAQLKVDDLEVEFVGARKESYRLDS
                     RKPIVEDGTLDDDLQRRDFTINTLALSLNKDTFGQLVDPFNGLKDIKNRIIRTPLDPL
                     TTFSDDPLRIMRAIRFATQLNFSIEPETLNAMLSMHERLEIVSMERKVDEVNKLMMAQ
                     RPSVGFLLMEQTGILEQLFPELAALKGVETINGISHKDNFLHTLQVLDNVAAKSDNLW
                     LRWAALLHDVAKPKTKKYIEGQGWTFYGHDYMGSRMVSGIFRRLKLPMNERMRYVEKL
                     VQMHLRPINLSQEEVTDSAVRRLLFEAGDDIDDLMTLCEADITSKNDSTVRRHLANFR
                     LVREKLKIIEEKDAIRNFQPPISGNIIMETFGIEPCHEVGVIKEAIKNAILDGKIGNN
                     FNEAYRLMLEKGMELGLKPR"
BASE COUNT          559 a          492 c          522 g          498 t
ORIGIN      
        1 gaaaccccac cagggctggt atagctacca gctctaatgg ggtatttgcc tgtaatgcca
       61 gaactagctg caggaagctc tcccctactc cactcccaaa acgtccttaa aggccttttt
      121 aaaggcatcc tttggtaggg cacccatggc catctggggt tggtcgttga ggggtataaa
      181 cagcagcgag ggtatgcttc gtatgccgaa aacccctgcc aactcctgct cggcctccgt
      241 gtcaaccttg taaatgttga gcttgccgtc atactccttc gagagctcct ccagaatggg
      301 ggcaaccatc ttacagggac cacaccagtc ggcatagaaa tcaacaatgg ccggcctgct
      361 gccctcatac ttccactccg tgtttttctc gtagtcaaaa actttctgct taaacgtctc
      421 cgctgttaaa tgttcaatca tggttatgca atttagtaac ccacaaatgt agttattttc
      481 atatttatat acgacattga ttaaacgaaa ttttgccggg ttggttcaac ataacaccaa
      541 aaaaaattca ttcaacccgc cagtcctatc tgtttgaacc aacatgcaag gcttcagcat
      601 tatttagccg ttgaaacagg ttgtcagaat ttcaattagt agtaattttg ccttctaaca
      661 tggaggaaat gcagcagtat cttaaacata aggttttttc ggttgtttcg cgtgtggctg
      721 aagttgagga ggtaagggtt tacgtcatcg gcggatatgt gcgcgatatt tttcttaacc
      781 gcacatcgaa ggatatcgac attgtggtgg tgggtagcgg gatagatttt gcccaaaggg
      841 tagcgcgcct gatgcccgga gccagaatgg cctacttcaa aaactttggc accgcccagc
      901 taaaggttga cgacctggag gtggagtttg tgggtgcccg taaggagtcg tacaggctcg
      961 attcgcgcaa gcccattgtg gaggatggca ctctcgacga cgacctgcag cgcagggact
     1021 ttaccatcaa cacattggcc ttaagcctca acaaggatac ctttggccag ctggttgacc
     1081 cattcaacgg gcttaaggat attaagaacc gaattatccg cacaccactc gatccgctta
     1141 ccaccttttc ggacgatccg ctaagaatta tgcgcgccat acgatttgcc acccagctga
     1201 actttagcat agagcccgaa acgctaaacg ccatgctgag catgcacgaa cgcctcgaaa
     1261 ttgtctcaat ggagcgaaaa gttgacgagg tgaacaagct gatgatggcc caacggccat
     1321 cggtcggatt cctgctaatg gagcaaaccg gaatacttga acagctgttc ccggagctgg
     1381 ctgcgcttaa aggggttgaa accatcaacg ggatatcgca taaggataac ttcctgcata
     1441 ccctgcaggt gctggacaat gtagctgcca aatcggataa cctttggcta cgctgggctg
     1501 cgctgctgca cgatgtagca aaacctaaaa cgaagaagta catcgaagga cagggttgga
     1561 cattttacgg tcacgattac atgggtagcc ggatggtttc gggaattttc agaaggctga
     1621 agctaccgat gaacgagagg atgaggtatg tggagaagct ggtgcagatg cacctgcgcc
     1681 ctatcaacct gtcacaggag gaggtaaccg attcggcagt ccgtcggttg ctatttgagg
     1741 caggcgacga cattgacgac ctgatgaccc tatgcgaggc cgacatcacc tcaaaaaacg
     1801 acagtaccgt tcgacgccat ctggccaact ttagactggt tcgtgaaaag ttgaaaataa
     1861 tagaggaaaa ggatgccatt cggaattttc agcctccaat cagcggcaat atcatcatgg
     1921 aaacctttgg cattgagcca tgccatgagg ttggggtaat taaggaggcc attaaaaatg
     1981 ccattcttga tgggaaaata gggaataact tcaacgaagc ctaccggttg atgctggaaa
     2041 aggggatgga gctggggcta aagccacgaa a
//
LOCUS       DAOSHM010000151         2049 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_54574_length_2049_cov_7.612337, whole genome shotgun sequence.
ACCESSION   DAOSHM010000151 DAOSHM010000000
VERSION     DAOSHM010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54574_length_2049_cov_7.612337"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            198..>2049
                     /locus_tag="PK839_10865"
     CDS             198..>2049
                     /locus_tag="PK839_10865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG73276.1"
                     /translation="MSKVRENLLVVVLVLFIARMGLPQYLKYILFPAVGLYGVWALWQ
                     FVEDRRWQGFRLRNTLIFTPLLVSMLIYFIMLVFTPNPQINLLRDAFNVLVFFSFVIA
                     LYLISYTPSGYQKVLNQVALYTIIISSLFAFLGVLKLVLQLYGITFEFLEVEMLGYPL
                     GTSLSVDNNSFAILCLLGLVLAIPYTTRKLKIRYSLLLQLGLTLIVVSAFFSTSRRGL
                     IIALLCLLICGLTWLVSIPFRSERLKNLRVNTSFFLLLSIMVIGNFYWFVNHMSPIER
                     YRFLYSHHFEKFEAIHFINRMAVQEQLISNGNTEYSDVEWKLWGTEFDPRYPYTGWAE
                     NNFKLVGEIKGKGAELVPEGAEAALVDSSVQGSTWGGHAYYYSILFEAKGEAGSWYMA
                     SVYCYVSPDFSGDNVEIGVEHAISSTTEKAIYDLQASGSWQKLEVTFQADTAAYKVGL
                     FMRVNNALNIKRLKGFMEFAYPELYKIQTHREELLASKVENKVDQQQDMQNVNPMEEE
                     VAQNPEEEQSGWFSSAARVLRNFLSIADSSGQFRHSMSNDFFSGPRVDRWRYALYIYR
                     YEYSPVQKIFGGGFGYTRKFTDMFRDKWRVTEYDYPHSPFLSVMLYSGIFG"
BASE COUNT          538 a          390 c          480 g          641 t
ORIGIN      
        1 ataatttttt tgaacccgca atatttggtg ttttggcatt tatcccattt tattacaaat
       61 acttatctgt tgtgaaggat taacaagttt aaataggtgt taggtgcgct ttatgcttct
      121 aatagtctat atataaggcg aataacatag aaaattagat gaatgtagat ggcggaatag
      181 tagtaacaat ttaattcatg tcgaaagtaa gggaaaatct attggtcgtg gtgctggtgc
      241 tttttatagc caggatggga ttaccgcaat atctgaagta catccttttc ccagcagttg
      301 gtctgtatgg cgtttgggcg ctatggcaat tcgttgagga cagaagatgg cagggtttta
      361 ggctaagaaa caccctgatt tttaccccac ttttagtttc catgctgata tactttatta
      421 tgctggtgtt tactcctaat ccgcaaatca accttttacg ggatgctttc aacgtgttgg
      481 tatttttttc ttttgttata gcgctatacc taattagcta caccccatcg gggtatcaaa
      541 aggttttgaa tcaagtagca ctctatacca ttatcatatc ttcccttttc gccttcctgg
      601 gagttttgaa gctggttttg cagctctacg gaataacatt tgagtttcta gaggttgaaa
      661 tgctgggtta tcctttagga acatcgctaa gtgttgataa caactccttt gcaatacttt
      721 gcttactggg actggttctc gctataccat atactaccag aaaactcaaa attcgatata
      781 gcctattact ccagctgggg ttaacattga ttgttgtaag tgcatttttt tctacctctc
      841 gaagaggact gattatcgca ttgctctgct tgctgatttg cggcttgacg tggttggtgt
      901 ccatcccatt taggagtgag aggttgaaaa accttagggt taacacatcc ttttttttgc
      961 ttctatccat aatggtaatt ggcaactttt actggtttgt caatcatatg tcccctattg
     1021 agagatacag gtttctatac agtcaccatt ttgaaaaatt cgaggcaata cattttatta
     1081 ataggatggc cgttcaggag cagctcatct cgaatggtaa cacggagtac tccgatgttg
     1141 agtggaagct ttggggaacc gaattcgacc ctcgttaccc ctatacaggc tgggcggaga
     1201 ataactttaa gctggtgggt gaaattaagg gcaaaggggc agaactggtt ccagagggtg
     1261 ccgaggctgc tttggttgac agcagcgtac agggttcaac ttggggtgga catgcctatt
     1321 actactccat acttttcgaa gctaagggcg aagcaggctc ctggtatatg gcatcggttt
     1381 actgttatgt ttctccagac tttagtggtg ataatgtgga aattggagtg gaacatgcca
     1441 tatcctcaac caccgaaaaa gctatttatg acttgcaggc cagtggtagc tggcagaagc
     1501 tggaggtaac ttttcaggcc gatactgccg catataaggt tggcttgttt atgagagtaa
     1561 acaatgcgct taatatcaag aggctgaagg gattcatgga atttgcctat cctgaactat
     1621 ataaaattca gacccatcgg gaagaactac tagcctccaa agttgaaaat aaggtagatc
     1681 agcagcaaga tatgcaaaat gtcaacccta tggaggaaga ggttgcccaa aaccccgagg
     1741 aggagcagag cggttggttt agtagtgctg caagggtgct acgcaatttt ttgagcatag
     1801 ctgattcgtc gggacagttt aggcactcca tgagcaatga tttcttttct ggtcctcgag
     1861 ttgataggtg gaggtacgcc ttgtacatct acaggtatga gtactcaccc gttcagaaaa
     1921 tttttggggg tggatttggc tacacccgaa agtttactga catgtttcgc gacaagtggc
     1981 gggttaccga atacgattac ccacacagcc cctttctttc tgtaatgctc tactcaggaa
     2041 ttttcggtc
//
LOCUS       DAOSHM010000152         2041 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_54895_length_2041_cov_11.919436, whole genome shotgun
            sequence.
ACCESSION   DAOSHM010000152 DAOSHM010000000
VERSION     DAOSHM010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54895_length_2041_cov_11.919436"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            264..1904
                     /locus_tag="PK839_10870"
     CDS             264..1904
                     /locus_tag="PK839_10870"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOG73277.1"
                     /translation="MTQISAVIIAYNEEDYIERCIKSVQGVADEVLVVDSFSTDKTGD
                     ICRQLGVRFIRHPFEGYREQKNYAMQLATYDYILSLDADEALSEELRQSILNVKENWG
                     ADGYYFNRLNNYCGQWIHHTNWYPDRKLRLFDRKKGSWGGVNPHDRVVMQKGSKVHYL
                     KGDMLHWVHSSYEAHIEKANKFSTIAAHEYFKLGKKSSIFGIFYHSSWCFFKAYVIRL
                     GFLDGYNGFVVSSLTAYTRFLKYVKLRHLNLQHKSESLSVERIAKKEFVPLTSQPASG
                     KPIATIIITTYNQPEWLKKVLWGFSMQTFSGFEILIADDGSDEKTKAVIEEFSRTGKF
                     RLKHVWHPDMGFRKCTIINKAITQASSDYLIFTDGDCIPRNDFVETHVKYARRGYFLS
                     GGYFKLTQPVSVTITHDDITMQKPFKASWLLHQGQPFTYKLLKFTRSRFLQHLLNTIT
                     TARATWNGHNVSGWKADIVAVNGYNENMQYGGLDRELGERLVNYGLKGKQIRYSAACI
                     HLDHPRPYKTQLTLDQNKQIRKDVRRNKITWTSNGIIK"
BASE COUNT          610 a          463 c          431 g          537 t
ORIGIN      
        1 atacctcagg cccaagcctc aacattctca tcaagttgat gagcatggtg tccatagtta
       61 tggcgggggt tacagtggcc ttcagcattt tttaggaata gccatgaatc ctttaaaagc
      121 ccagcatatc cagctgggct tttttctctc taccccctgc atcccctcta acctgatggc
      181 aattaaattc gttaaaccag caaccgtgtt attgaaaaaa tcttactttt acacgccatt
      241 tgtcagaatt atttcaaaat aacatgaccc aaatttcagc cgtaatcata gcttacaacg
      301 aggaggatta cattgaacgt tgcataaaat ccgttcaggg cgttgccgat gaagtgctag
      361 ttgttgactc attctcaacc gataaaaccg gcgatatttg ccggcagctg ggagttaggt
      421 ttatccggca cccctttgag ggttaccgcg agcagaaaaa ctatgctatg cagcttgcaa
      481 cctatgacta cattctgtct ctcgatgccg atgaggccct ttcggaagag cttaggcaat
      541 ccatactaaa tgtaaaggag aactggggtg ctgatggcta ctactttaac cggttaaata
      601 actactgtgg acaatggatt caccatacaa actggtaccc cgacaggaag cttagacttt
      661 ttgacaggaa aaagggcagc tggggtggag taaacccaca tgacagggta gttatgcaaa
      721 agggaagtaa ggtgcattat cttaagggtg atatgctaca ctgggtgcat agcagctacg
      781 aggcacacat cgaaaaggcc aacaagttta gcaccattgc agcacatgaa tacttcaagt
      841 tgggaaagaa aagcagcatt ttcggtattt tctaccacag ttcatggtgc ttttttaaag
      901 cttacgttat ccggcttgga tttctcgatg ggtataatgg ttttgtggta agctcgctga
      961 cagcctacac gcgtttttta aaatacgtaa aattacgcca tttgaacctt caacataaat
     1021 ccgagagttt gagcgtagaa agaattgcaa aaaaagaatt cgtacccctc acttcgcaac
     1081 cagcatccgg gaaacccatt gccaccatca ttattaccac ctataaccag ccggaatggc
     1141 taaaaaaggt actctggggt tttagcatgc aaaccttctc cggatttgaa attctcatag
     1201 ccgatgacgg ctctgatgaa aaaaccaaag cggttattga ggaattttca agaaccggga
     1261 agttccggct aaagcatgtt tggcatcctg atatgggctt ccgtaaatgc acaattatta
     1321 acaaggctat tacccaggca tcgtccgatt acttaatttt tacggacggg gattgcattc
     1381 ctcgaaacga ttttgtggag acccatgtaa aatatgcccg tcggggttac tttctaagtg
     1441 gcggctactt taaacttacc cagcctgtct ccgtgaccat cacccatgat gatattacca
     1501 tgcagaaacc attcaaggca tcttggctgc ttcaccaggg gcaacccttt acctacaagc
     1561 tacttaagtt cacccgcagc aggtttctgc aacacctcct gaataccata actactgccc
     1621 gtgccacatg gaatgggcat aatgttagcg gctggaaagc agatattgtt gcagtaaacg
     1681 gctataatga gaacatgcag tacgggggct tagaccgtga acttggcgaa aggcttgtca
     1741 attacgggct aaaaggaaag caaattaggt acagcgctgc ctgtattcac ctggaccatc
     1801 cacgtccgta taaaacacaa cttaccctag accaaaataa acaaatccgt aaggatgtaa
     1861 ggagaaataa aattacctgg acatccaacg gtattatcaa gtaactctat acatccatat
     1921 ttatgccaag accagttgcc tccctgatta tttcaaccta caagaacgta gaatttctta
     1981 atgctgtcat aaaatcggtg ttcaggcaaa ccgacagacg agttgaaatt attatttctg
     2041 a
//
LOCUS       DAOSHM010000153         1963 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_58270_length_1963_cov_20.935535, whole genome shotgun
            sequence.
ACCESSION   DAOSHM010000153 DAOSHM010000000
VERSION     DAOSHM010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1963
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58270_length_1963_cov_20.935535"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(178..1593)
                     /locus_tag="PK839_10875"
     CDS             complement(178..1593)
                     /locus_tag="PK839_10875"
                     /inference="COORDINATES: protein motif:HMM:NF021596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HOG73278.1"
                     /translation="MVRKIFFIPAGLILLFTSVVVLNSCAGKSTQGEDLSFAELSPAQ
                     RDEFINDLKSRTFRYFWEVVDTNTWQNDDRFPTYNFTSIAATGFALPSYIIGVENNFI
                     SRQQASERVLNTLEWLWSSKQGAEPDATGYKGFYYHFLSYGTGRRYDKVELSTIDTGL
                     LLAGILTCQTYFDGADKTEVRIRELADSIYLRVDWQWAMNGAANMSMGWTPEQGFIQS
                     TWNGYNEAMILLIMAIGSPTHPIDSTAWDSWCSTYKWDSFYGYEHVPFGPLFGHQYSH
                     QFIDFRGIQDSYMRSKGIDYFENSVRATLSNRAYCMENPLKFEGYSANMWGLTACDGP
                     AMAKIRANGRVVQFYDYRARSACSYEIIDDGTIAPTAAGGSIPFAPEECLSALYTMKT
                     MLGSNVYREYGFVDAFNLTVKNGSQGWFDRDYLGIDQGPILIQLENYQSGLIWNLMKK
                     NRYIVSGLKKAGFRNGWLEQS"
BASE COUNT          537 a          454 c          399 g          573 t
ORIGIN      
        1 tttactccgg gcagcgtttc gccagtttcg gcatcggtaa cccttccgct cactgttttt
       61 tgtgcaaatg cttcgggcaa gcatagcaca agtagtaagt ttaacagcag aaacttctta
      121 aaatgaactt ttctctccat ttgcataggt ttaaaagttt aggtttgata tcttggttta
      181 cgattgctct aaccaaccat ttctgaaacc ggccttcttt agacctgaaa ctatatacct
      241 gttctttttc atcaggttcc aaatcagccc gctctggtag ttttccagct gaataaggat
      301 tggtccctgg tctattccca ggtaatctct gtcgaaccaa ccctggctgc catttttcac
      361 agtcaggtta aacgcatcaa caaagccgta ctcccggtaa acattgctac ccagcatagt
      421 cttcatggta taaagagcac tcaggcattc ctcgggggca aatgggattg accctcctgc
      481 tgctgtagga gcaattgttc catcgtcgat aatctcgtag ctgcaagcgc ttcttgcccg
      541 gtagtcgtaa aactgcacga ccctgccatt agccctgatt ttggccattg cagggccatc
      601 acacgccgta agcccccaca tgttggccga atacccctca aattttagtg gattttccat
      661 gcagtaggcc ctgttgctca gggtagccct taccgagttc tcgaagtagt caattccctt
      721 tgaacgcatg tagctatcct gtattcccct gaagtcgata aattgatggg agtactgatg
      781 gccaaagagt ggcccaaagg gcacatgctc atacccataa aaactatccc atttgtaggt
      841 gctgcaccag ctgtcccatg cggtggagtc tatgggatgg gttggggagc cgatagccat
      901 gatgagtaga atcatggcct cattataccc gttccaggtg ctctgtatga atccttgctc
      961 gggagtccag cccatggaca tgttggctgc accattcata gcccattgcc agtcgacgcg
     1021 caggtaaatg gaatcggcaa gctcccgtat tctaacttcg gttttgtcgg ccccatcgaa
     1081 gtacgtctgg caggtaagta ttccggccag gagcaagcca gtatcaatag tcgaaagctc
     1141 aaccttatca tatctcctgc cggtcccgta cgaaaggaag tggtagtaaa agcctttata
     1201 tccggttgcg tcgggctcag ctccctgctt tgaactccaa agccactcca gggtgttgag
     1261 cactcgttcc gaagcttgct gacgggaaat aaaattgttt tctacaccta ttatataaga
     1321 gggaagggca aaacccgttg cagcaatgct ggtaaaattg taggttggaa agcggtcgtc
     1381 attttgccag gtattcgtgt caaccacctc ccaaaagtag cgaaatgtcc tactttttaa
     1441 gtcattaata aattcatccc tttgagcagg acttagctca gcaaaagaaa gatcttcccc
     1501 ttgagttgat ttaccagcgc atgaatttaa aacaacaaca gaggtaaata acagtattaa
     1561 gcctgctgga ataaaaaaaa tttttctaac catagttgaa gcaaaaaatt tacaaatcat
     1621 tatatccttt aacttgattt agctcaaagt taacactcga taaaacgttt gtccagaatt
     1681 ccgcctcatc atggctacgg ttataacaaa agggaatttt ttaagcatgc ttaacagtac
     1741 gtcattttta atatttaaac tttattatac tgtttttctg atactttaga attatgattt
     1801 atagaaatat ttttagccga aaacgatttc ggcgaaacca tttaattaca cctataaaat
     1861 attaacatat tgattttgtg gtttttaatt cattatgata aaatttagac gatttataac
     1921 ctaaccatag ggtatgacta agtcagtgct acccatgccg cta
//
LOCUS       DAOSHM010000154         1748 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_69505_length_1748_cov_17.296515, whole genome shotgun
            sequence.
ACCESSION   DAOSHM010000154 DAOSHM010000000
VERSION     DAOSHM010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1748
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69505_length_1748_cov_17.296515"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            118..1275
                     /gene="mqnC"
                     /locus_tag="PK839_10880"
     CDS             118..1275
                     /gene="mqnC"
                     /locus_tag="PK839_10880"
                     /EC_number="1.21.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic dehypoxanthinyl futalosine synthase"
                     /protein_id="HOG73279.1"
                     /translation="MDLESLYRKALALEPLSMEEGSYLYRHAPTHDLIYLGNELRQLH
                     VPSKKVTWQIDRNVNITNVCISGCKFCNFHTGLHSNNAYITTFPEYRQKVEELERYGG
                     DQLLLQGGLHPKLGLEFYVNLFTQLKSAFPNIRLHALGPPEIAHIAKIDGLSNSEVLQ
                     KLVESGLDSLPGAGAEILVDRVRKRISPGKPDSQSWLDVMAEAHKMGLVTSATMMIGH
                     VETIEERVEHLIKLRDLQNRKPTNAPGFVNFIVWTFQGKGTMLEKEGITPHVTDEEYI
                     RTIAISRIMLNNIKNIQASWLTVGIEVAQVCLHAGANDLGSIMIEENVVSSAGAKYRT
                     DAYGIQKAIADAGFEPQLRNQRYEFVDLPKCEYSRIYSLNELKNHPVNPAG"
     gene            1553..>1748
                     /locus_tag="PK839_10885"
     CDS             1553..>1748
                     /locus_tag="PK839_10885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG73280.1"
                     /translation="MKKILLAVLLGFPIMVAAQNNEAGKQEKVKTGWNLGGLPVVAYD
                     TDLGLEYGGLVNLFYYGDGSL"
BASE COUNT          489 a          434 c          401 g          424 t
ORIGIN      
        1 ccagcataga aatttaccaa agttgtcgct aaccaatcta cctataaagt aggccatagc
       61 tcaacctcaa cccataaaaa ttaaatagct aattttacaa aattttacga gacacaaatg
      121 gatctggaat ctctttaccg taaggctctt gcgcttgaac ccctatcgat ggaagagggt
      181 tcatacctat accgtcatgc cccaacccac gacctgatat accttggcaa cgagcttcgc
      241 cagcttcacg tcccatcgaa aaaggtaacc tggcaaattg accggaacgt taacattacc
      301 aacgtctgca tttcgggttg taagttttgc aacttccaca ccggtctgca ttccaacaat
      361 gcctacatta ccacttttcc cgagtacagg cagaaagtgg aggaactgga gcgctacgga
      421 ggcgatcagc tcctgcttca gggcggccta catcctaagt tgggtctaga attctatgtt
      481 aatcttttca cccagcttaa aagcgctttc cccaacatca ggctgcacgc acttgggcca
      541 ccagagatag cgcatatagc aaaaatagat gggctatcca acagtgaagt tctgcaaaaa
      601 ctagtggaat cggggttgga cagcctgcct ggtgcaggag ctgagattct ggttgaccgg
      661 gtaaggaaaa gaatttcgcc cggcaaaccc gatagccagt cgtggctcga cgttatggcc
      721 gaagcacata aaatggggct ggttacatcc gccaccatga tgattggaca cgttgaaacc
      781 atcgaggagc gggttgagca cctcatcaag cttagagatc ttcaaaaccg taaaccaacc
      841 aatgcccccg ggtttgttaa ctttatcgtg tggacattcc aaggcaaggg caccatgctc
      901 gaaaaggagg gcatcacccc gcacgttacc gacgaggagt acatccgcac cattgccatt
      961 agccgcatca tgcttaacaa tattaaaaat attcaagctt cctggctaac tgttgggatt
     1021 gaggtagcac aggtctgcct tcacgccggg gcaaatgatc tcgggtccat aatgattgag
     1081 gaaaatgttg tttcgtcagc cggagcaaaa taccgcaccg atgcctacgg catacagaag
     1141 gctattgccg atgcaggatt tgaaccccag cttcgtaacc aaaggtatga gtttgttgac
     1201 cttcccaaat gcgagtatag ccgaatctac agcctaaacg agcttaaaaa tcacccggtg
     1261 aatccggcag gttaggccaa gcaaaatggc attactacaa cacctggctt gattgcccat
     1321 accatttagc ctatattcct ggcatttaac tttctacgcc aataaaagat tgaagcgggc
     1381 aggtttatcc cctgcccttt ctgcttcaaa tgcgttcgcc gtaacaacat gcggtatatt
     1441 tgtgctgcta ttcaactctt ttgagtaggc attatgaaca aaattgcccc attgagatga
     1501 accggtatcg acattttaca gtaacttggt gcaacgataa acacgttacg ggatgaaaaa
     1561 aattttgctg gctgtcctgc tgggctttcc gattatggtt gctgctcaaa ataatgaagc
     1621 cggtaagcag gaaaaagtaa aaaccgggtg gaaccttggc gggttgcccg tggttgccta
     1681 cgacaccgac ttaggcctgg agtacggagg gctggttaac ctgttctact acggcgatgg
     1741 ctcgcttt
//
LOCUS       DAOSHM010000155         1745 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_69687_length_1745_cov_16.856213, whole genome shotgun
            sequence.
ACCESSION   DAOSHM010000155 DAOSHM010000000
VERSION     DAOSHM010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1745
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69687_length_1745_cov_16.856213"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(189..1568)
                     /locus_tag="PK839_10890"
     CDS             complement(189..1568)
                     /locus_tag="PK839_10890"
                     /inference="COORDINATES: protein motif:HMM:NF014147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 30 beta sandwich
                     domain-containing protein"
                     /protein_id="HOG73281.1"
                     /translation="MKLHSNFAILLVATAMMACNSPKDRVEVWVSKADSTILLEQQPG
                     INFSNNQIGEVIEVDTNQMCQMINGVGAALTWSSAYVFKNYLTPETRLQLFRELFTPE
                     GIGINVVRLTIGSSDFSRGNHSYSETPDTSLSNFTLAMDMDEVVPMMKEILAVNPNIT
                     IIASPWSPPAWMKSSNSMVGGNLLKQHYGAYANYLVKYIKAMQEQGIPIYGITVQNEP
                     EFGTATYPCMLMSAQEQNEFIRYYLGPRMAQNGLTTRIILFDHNCDSPQYPISILNDS
                     ITRKYVDGSGFHLYKGEIEALCTVHEAHPDKNIYFTEQSGGGWAPNFGDNLLWYSETL
                     MVKAMRCWSKTVLLWNLALDENDGPKNNGCSNCWGVVQVPSNGAVKRNAEYYALGHFG
                     KFVLPNATRLVSTMQKVSNVAFLNPDGSKVLIAVNTSGQPVAFTVKCGGRQFIYQMGG
                     REVVTFRWK"
BASE COUNT          441 a          416 c          414 g          474 t
ORIGIN      
        1 tttcaggata agctcacttg cccggcaaca agcgtagccg ccggatattg cctgcattcc
       61 tacactgccg cacgaaaacc ccatcgtgcg gtttactttt tatcgttaaa atactcgcgt
      121 aaacactgac attctagctg gggtttgggc gagtattcag gcatggccac acactgcacc
      181 tgataaacct acttccagcg gaacgtaacc acctcacgtc cacccatctg gtagataaac
      241 tgcctgccgc cgcacttcac ggtaaaggca acgggttgtc cggatgtatt caccgcaatc
      301 agcacctttg aaccatcggg gttcaggaaa gccacgttgg ataccttttg catggtggag
      361 accagccgtg tggcgttggg tagaacaaac tttccgaaat ggcccagggc atagtactcg
      421 gcattcctct taacggctcc gttggagggc acctgaacaa ccccccagca gttggagcaa
      481 ccgttgttct tggggccatc attttcatca agggcaagat tccatagcag aacggttttg
      541 ctccagcacc gcatagcttt caccataagg gtttcggagt accagagcag gttgtcgccg
      601 aagtttggcg cccatccccc acccgactgt tcggtgaagt agatattctt atcgggatgg
      661 gcctcgtgaa ctgtgcaaag ggcttctatt tcgcctttgt agaggtgaaa acccgaacca
      721 tccacatact tccgagtaat ggaatcgttc agaatgctaa tgggatactg cggggagtcg
      781 cagttatggt caaatagtat tattcgggtt gtcaggccat tctgtgccat ccggggtccc
      841 aggtagtagc gaataaactc attctgctcc tgagcgctca taagcatgca ggggtaggtg
      901 gccgtgccga actccggctc attttgaaca gttattccgt aaatgggaat tccttgctcc
      961 tgcatggctt tgatgtactt taccaggtag ttggcataag caccatagtg ctgcttaagt
     1021 agattccctc ctaccatgct gttggatgat ttcatccatg ccggtgggct ccacggcgag
     1081 gcaataatgg tgatgttggg gtttacagct aatatttcct tcatcatggg aacaacctcg
     1141 tccatatcca tggccaatgt aaagtttgaa agagatgtat cgggcgtttc gctgtagctg
     1201 tggtttcctc gtgaaaaatc agaggagcca atagttagcc taaccacgtt gatgccaata
     1261 ccctcagggg taaacagctc gcgaaaaagc tgcaggcggg tttcaggagt gaggtaattc
     1321 ttaaaaacgt aggccgatga ccaggtaaga gccgcaccta cgccattaat catctggcac
     1381 atttgatttg tatcaacctc aataacttca ccaatctggt tgttggaaaa attaatgccg
     1441 ggctgctgtt ctagtagtat ggtagaatcg gcttttgaaa cccagacttc aaccctatct
     1501 ttcggggagt tacaagccat catggcggtt gcaaccagca agatggcaaa attcgaatgc
     1561 aacttcatag tgattatttt tttgtaagtt taactaaaac cctaaatttt taggtaatag
     1621 atattttcat tttctccagc agttttttgt tggtttatta attccggcaa aagctaatcc
     1681 gttaggtgca aattttattc tctttaccga cttaatacca gggtgcattc aaagatgcaa
     1741 aatta
//
LOCUS       DAOSHM010000156         1716 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_71439_length_1716_cov_6.638170, whole genome shotgun sequence.
ACCESSION   DAOSHM010000156 DAOSHM010000000
VERSION     DAOSHM010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1716
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71439_length_1716_cov_6.638170"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            102..>1716
                     /locus_tag="PK839_10895"
     CDS             102..>1716
                     /locus_tag="PK839_10895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG73282.1"
                     /translation="MKKFPGVNAEIVPKGALGARIDKNAKAYTGKDYAYYLSKLFDGE
                     LAENKRCISSVYCFVSEDFNGSSVRLITRNDARGLRDWFYDLGKKGTWQKLQNSFYGD
                     SGRFSTSIYLVNENSQSFDSLQGHVIFAYPQLKEIEFNPKDPLTWASNQFVEVSELHG
                     FNATKIPQGTKALKPIKADIKYREKDSTWYYGAGLFRYHFQDTTRVVPSIYVYVSTDF
                     NGDEVYLNAGGRIYGFTKCNYNLELKGTWQKLYLTYSMFEGESWVDYGYRKKVTSPID
                     TVKGYVLFANPDQMFLKFDSKKPLTWAGTNFTSITTLTGKGSEQIPMGSIGLKVDKNS
                     QSKSYKGLVYTTNSIIYLKLVNSLQRLKTSIYVYASPDFNGQNLRLAGSTKNIGGYWT
                     SNYNMDNKGTWQKLTINNWGEAGEEYYSTTYFELPNAKDFKNLKGYVIFAHPDFKLLY
                     FDPKNPESYTSSAFIRDYPLVGNNSDIVPQGVAGARFNRTTEGKVWRDFYHSTTLFCG
                     VQAEPGDSVFASVYCFVSPDFNGNDVRLEL"
BASE COUNT          541 a          294 c          378 g          503 t
ORIGIN      
        1 agggcaatgc acaatatacc gatgtgagtg ataaaatctg gcaaacaaca tttgaccccc
       61 gatacccata cacgggttgg gcccgtggta actacgagtt ggtgaagaag tttccggggg
      121 ttaatgctga gattgtaccc aagggtgcgc ttggtgcgag gattgataag aacgctaagg
      181 cttacacagg taaagattac gcatattacc tatccaaact atttgatggg gaactggcgg
      241 agaataaaag atgcatttcg tcggtgtact gcttcgtttc ggaagacttc aatggaagca
      301 gcgtgagatt gattacccga aatgatgcca gggggttgcg cgactggttc tacgatttgg
      361 gtaaaaaggg aacctggcag aagctgcaga attcctttta tggcgattcg ggcaggtttt
      421 cgacatcgat ttacttggtg aatgagaatt cccagtcgtt tgatagcctt cagggccatg
      481 ttatttttgc ttatcctcaa ctcaaagaaa ttgaatttaa ccccaaagat cctcttacat
      541 gggcaagcaa ccagtttgtt gaagtaagtg aattacatgg ctttaatgca actaaaattc
      601 ctcagggaac taaagcccta aaaccaataa aggctgatat taaatatcgg gaaaaagata
      661 gtacatggta ttatggggcg gggttattcc gttatcactt tcaggatacg acaagagtag
      721 tgccctcaat atacgtttat gtatcaaccg atttcaatgg cgatgaggtt taccttaatg
      781 caggaggacg tatttatggt tttacaaaat gcaactataa tctcgagcta aagggtactt
      841 ggcaaaagtt gtatttaact tatagtatgt ttgagggtga aagttgggta gattatggat
      901 ataggaaaaa agtaacatct cctattgaca cagtaaaagg atatgtttta tttgctaatc
      961 ctgaccaaat gtttttaaaa tttgattcaa aaaaacctct aacatgggca ggtacaaatt
     1021 ttacttcaat caccacttta acaggtaaag gaagtgaaca aatacctatg ggtagtatag
     1081 gtttaaaagt tgataaaaat tcgcagtcaa aaagttataa gggtttagtt tacacaacta
     1141 actctattat atatctaaag cttgttaatt cattacagcg tttaaaaaca tctatttatg
     1201 tatatgcttc tcctgatttt aatggtcaaa acttacggct agctggtagt actaaaaata
     1261 ttggtggtta ttggacatcg aactacaaca tggataataa aggtacatgg caaaagttaa
     1321 ccattaataa ctggggtgaa gcaggagagg aatactactc aacaacctac tttgaattgc
     1381 ccaatgctaa agattttaaa aacctaaaag gttatgtaat atttgcccac ccagatttca
     1441 agctactata ctttgaccct aagaatccag aaagttatac atcaagtgct tttattcggg
     1501 attatccatt ggtgggcaat aactccgata ttgttccaca gggagtagcc ggggctaggt
     1561 tcaatagaac aactgagggg aaagtctgga gagattttta tcattctact acattatttt
     1621 gtggtgtaca ggcagaacct ggcgattcgg tatttgcttc agtttactgc ttcgtttcac
     1681 ccgatttcaa cggtaacgat gtgcggctgg agctgc
//
LOCUS       DAOSHM010000157         1689 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_73154_length_1689_cov_22.577723, whole genome shotgun
            sequence.
ACCESSION   DAOSHM010000157 DAOSHM010000000
VERSION     DAOSHM010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1689
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73154_length_1689_cov_22.577723"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(134..1522)
                     /gene="lpdA"
                     /locus_tag="PK839_10900"
     CDS             complement(134..1522)
                     /gene="lpdA"
                     /locus_tag="PK839_10900"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HOG73283.1"
                     /translation="MNFDLIVIGSGPGGYVAAIRASQLGMKVAVVERENLGGICLNWG
                     CIPTKALLKSAQVYEYASHAADYGVKTTDVKPDFETMVARSRGVAETMSKGIQFLFKK
                     NEVTVINGFGKLKDNHTVSVTAPDGSTSDYTSSHIILATGARSRELPNLKQDGVRVIG
                     YRQALNMKRQPASMVVVGSGAIGSEFAYFYNAIGTKITLVEFLPNIVPVEDEEVSKAL
                     ERAFKKSGITVKTEATVESVDTSGELCRVSIQTKKGVEVVEAEVVLSAVGITPNIEGI
                     GLEELGISVEKGRVKVDEFYRTNVEGVYAIGDIIPGPALAHVASAEGITCVEKIAGLN
                     PEPINYNNIPGCTYTNPEISSVGMTEKAAVEAGYDVKVGKFPFTASGKATAAGQRDGF
                     VKLIFDARYGELLGAHLVGGNVTEMVAELVVARKLEITGHELIKSIHPHPTMSEAIME
                     AAAAAYGEAIHI"
BASE COUNT          385 a          462 c          391 g          451 t
ORIGIN      
        1 agggttagga aatttaaagc ccaagtgctt atggggcaga taccattatt tgccataaaa
       61 gcccatcccc aacggttact ggaatcgttg gggataggct gattaatcct gaagttgact
      121 agcggcgttc cggttatatg tggatggcct cgccgtaggc agcagcagcg gcctccatga
      181 tggcctcgct catggtggga tgcgggtgta tgctcttgat aagctcatgc ccggtgattt
      241 cgagtttccg ggcaacaacc agctccgcta ccatctcggt aacgtttccc ccaaccagat
      301 gggcgccaag cagctcgccg tatctggcat caaaaatcag cttaacgaac ccatcgcgtt
      361 gtccggctgc ggttgccttg cccgaggcgg taaatggaaa cttacctact tttacatcat
      421 acccggcctc cactgcagcc ttctcggtca tgcctaccga tgaaatctcg gggttggtgt
      481 aggtgcagcc gggaatatta ttgtagttaa tgggctcggg gtttagaccg gcaatttttt
      541 ccacgcaggt tatcccctca gccgatgcca catgggcaag cgcaggacct ggaattatat
      601 ccccaatggc atagacaccc tcaacgttgg tgcggtagaa ctcgtccacc tttacccgtc
      661 ccttctctac ggaaataccc agctcctcaa ggcctatccc ctcaatgtta ggggtaatgc
      721 ccacagccga aagcaccacc tctgcctcaa ccacctctac tccctttttt gtctgaatgc
      781 ttaccctgca tagctcgccg gaggtatcaa ccgactcaac ggtagcctct gttttgacgg
      841 ttatccccga tttcttgaat gctctttcca gcgccttgct tacctcctcg tcctcaaccg
      901 gaacaatgtt tggcaggaac tcaaccaggg taattttagt gccaatggcg ttgtagaagt
      961 aggcgaactc gctgccaatt gcgccggagc cgacaaccac catggaggca ggttgcctct
     1021 tcatgttcaa tgcctgccgg tagccaatca ccctaacgcc atcctgctta aggttgggca
     1081 gctcgcgcga acgggctcct gttgccagaa tgatgtggct tgacgtgtag tcggaggttg
     1141 aaccatcggg agcggttacg cttaccgtat ggttgtcctt tagcttccca aacccgttga
     1201 ttacggttac ctcatttttc ttaaaaagaa actgtattcc cttgctcatg gtttcggcaa
     1261 cgcccctgct ccgcgccacc attgtttcaa aatcgggttt tacatcggtt gtcttaaccc
     1321 cgtaatcggc agcatgtgag gcatactcat aaacctgcgc acttttcagt agtgcctttg
     1381 ttggaatgca accccagttg aggcaaattc ctcccaggtt ttcacgctcc acaacggcaa
     1441 ccttcatccc cagctggctg gcccgaatgg cagcaacgta gccgcccggt ccgcttccta
     1501 ttactatcag gtcgaaattc atatccggta atttttagtt tttcaaaagt agtataacaa
     1561 acggtgatgg gaaaatgtta ttggacataa catgttaatc ccaaaccctc caaaatcagg
     1621 taatatattt tcaaaaaaag cggaataatc gggtaaatag tttgtttatt cgattttttt
     1681 gttgtttct
//
LOCUS       DAOSHM010000158         1673 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_74272_length_1673_cov_21.771323, whole genome shotgun
            sequence.
ACCESSION   DAOSHM010000158 DAOSHM010000000
VERSION     DAOSHM010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1673
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74272_length_1673_cov_21.771323"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            144..1598
                     /locus_tag="PK839_10905"
     CDS             144..1598
                     /locus_tag="PK839_10905"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005866098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HOG73284.1"
                     /translation="MKGQKILIPIASAIIGGLVAVGISEGIYRNNRVNVATERDNNAT
                     VQLTRVFSGADSAYTDFTWAAEKTVDAVVHVTTSYTQTLDYSFGNPLFDFFFGPNGFD
                     QRRQVQSSGSGVILTNDGFIVTNNHVVENAEDITVVLNDKRKFSAKVVGADPNTDIAL
                     LKIDAKDLPTIPFGNSDDIKVGEWVLAIGNPFNLNSTVTAGIVSAKARNIQILSNREF
                     AIESFIQTDAAVNPGNSGGALVNLRGELIGINTAIASRTGTFAGYAFAVPSSIVKKVV
                     TDLIEFGEVRRAVMGVRIQELTDELAKEKGIKDIKGVYVADVEKNGAADKAGIKAGDV
                     ILSINNVDVNSTAQLQEQISRYRPDQQVDVLVNRDNTKKHYTVTLSNLKSEMKFSKSE
                     DAFSSLGATFAEVPAKLKRDLGIDYGLQVVELADGKLKDGGIRQGFIITRVNRTPIRS
                     EDDLKRVLSISSGGVLIEGVYPNGTVGYYAIGVD"
BASE COUNT          468 a          330 c          422 g          453 t
ORIGIN      
        1 ccctgttcat tttgtcagaa aattttcata aaaatatgaa aaactgacat acaggaggtt
       61 aacaagtgtt aaggaaaagg ttagggcggc ttagggtata ttttttgatt gctactttta
      121 caagaactta aaacgttaaa gctatgaaag gacaaaaaat tttaatacct atagcttcag
      181 ccattattgg tggtttggta gcagtgggca tttcggaggg catctatcga aataaccggg
      241 ttaatgttgc cactgagcgg gacaataatg ctacagtgca gctcacccgg gtgtttagtg
      301 gagccgattc agcctacaca gattttacgt gggcagccga aaaaacggtt gatgctgtgg
      361 tgcatgtaac cacatcgtac acccaaacgc tcgactactc tttcgggaat cccctgttcg
      421 acttcttttt tgggccaaac ggattcgatc aacgacgtca agtgcagagt tccgggtcgg
      481 gtgtaattct tacaaacgac ggttttatcg taaccaataa ccatgtggtg gaaaatgccg
      541 aggatattac cgtagtttta aacgataagc gtaaattttc tgcaaaggtt gttggagccg
      601 accccaacac cgacattgcc cttcttaaaa ttgatgccaa ggaccttccc accattcctt
      661 ttggtaattc cgacgacatt aaggtagggg agtgggtttt ggcaattggc aaccctttca
      721 accttaactc aacggttact gcaggtattg taagtgctaa agctcgtaac attcaaatac
      781 ttagcaacag ggagtttgcc attgaatcgt tcattcaaac cgacgcagct gttaacccgg
      841 gcaatagtgg tggtgcgctg gttaacctaa ggggtgagct cattggcatt aataccgcca
      901 ttgcttcccg tactggcaca tttgcagggt atgcctttgc tgttccctca tcaatagtta
      961 aaaaggtggt taccgactta atagagtttg gtgaggtgcg gcgtgctgtt atgggtgttc
     1021 gaatccagga gctaaccgat gagctggcta aggaaaaagg cattaaggat ataaaaggag
     1081 tgtatgttgc cgatgttgaa aagaatggtg ctgccgataa agcaggtatc aaagcgggcg
     1141 atgttatcct ttcaatcaac aatgttgatg taaacagcac tgcccagctt caggagcaaa
     1201 ttagccgcta ccggcctgac caacaggtgg atgttctggt aaaccgtgat aatactaaga
     1261 aacattacac cgttaccctt agcaatctga aaagcgagat gaagttttca aagtctgaag
     1321 atgccttcag cagtcttggt gcaacctttg ccgaggtacc agccaagctg aaaagggatt
     1381 tgggcataga ctatggcctt caggttgttg agctagccga tggcaagctt aaggatgggg
     1441 gcattaggca aggatttata attacccgtg ttaaccgaac accgataagg agtgaagatg
     1501 atttgaagcg ggtgcttagc atttcgtccg ggggtgtgct gattgagggt gtttacccca
     1561 acggaactgt tggttactac gcaattggtg tagattagaa ccaggaaaat atcttatagg
     1621 ttatataagg ccgcttatca agcggcctta ttggtatggt gtgtcgggca aac
//
LOCUS       DAOSHM010000159         1656 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_75482_length_1656_cov_10.870081, whole genome shotgun
            sequence.
ACCESSION   DAOSHM010000159 DAOSHM010000000
VERSION     DAOSHM010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1656
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75482_length_1656_cov_10.870081"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(330..1457)
                     /locus_tag="PK839_10910"
     CDS             complement(330..1457)
                     /locus_tag="PK839_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018966072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOG73285.1"
                     /translation="MNPKIWLSSPHMSGREMRYINEAFDTNWVSPLGPNVTGFEQELA
                     SYLKVNHCAALVSGTAALHMALINLGVAYGDEVVCQSFTFSASANPIVYQGATPVFVD
                     SEPETWNMDPNLLEVAIKDRIAKGKKPKAIIAVHLYGMPAKIDEILSVAQRYEIPVVE
                     DAAEAIGSTYRGKNCGTFGVMGALSFNGNKIITTSGGGALVSENEGYIKNTRFLSTQA
                     RDVAPHYQHSKIGYNYRMSNIVAGIGRGQLEVLNLRVQQRRANNQFYRNLLGSLEGIT
                     FLTESNGTFSNYWLTAMLIDPKIGISREDVRLGLEADNIESRPLWKPMHLQPVFSGAP
                     AYTSGFSEKAFEQGLCLPSGSNLTDVEKERIASQLKRIFRL"
BASE COUNT          429 a          425 c          337 g          465 t
ORIGIN      
        1 cgttaaccac caccttttct tttatcattt ttggtcatat ttttttagaa ggctaccctg
       61 ttggcagtga aaaacttcac tcgggttttt acccgccatt tccaatcatg gagggatgcg
      121 cttcaaaatg tgtagatgac cctgcaggtt agcggtaccc tcctcctaag cacctcagcc
      181 tctaccctga acctatggcg gcttaaaact ttctccattt tgcccacatg gtttccacca
      241 aacttagaca ctactttcaa cataaccagt cacactacta catgggacaa ctcccctttt
      301 gaaaaagggg gccgagggga ttactccctc tatagcctaa atatcctttt taactggctg
      361 gcaattcttt ccttttccac atcggtaaga tttgaacccg agggcaggca tagcccctgt
      421 tcaaaagctt tttccgaaaa tccactggta tatgccggcg cacctgaaaa aacaggctgt
      481 aggtgcatgg gcttccacaa cggacgtgac tcaatattat cggcttccaa gcctaaacga
      541 acatcctccc tactgatacc aattttagga tcaatgagca ttgctgtaag ccaataattt
      601 gaaaaggttc cgtttgactc agtaaggaag gttattccct ccaggctgcc gagcaggttt
      661 ctgtaaaact gattattagc ccttcgctgc tgaaccctta agtttagcac ctcgagctgg
      721 ccgcggccaa tgcctgcaac tatgttgctc atcctatagt tgtagccgat tttggagtgc
      781 tggtagtgtg gcgcaacatc tcgggcctgg gttgaaagga aacgggtatt tttaatgtac
      841 ccctcgtttt ctgataccaa cgctccaccg cctgaagtcg taataatttt attaccattg
      901 aacgacagcg cacccattac accaaaagtt ccacagtttt tccctctgta ggtactgcca
      961 atggcctcag cagcatcctc aactacagga atttcgtagc gctgtgccac agaaagtatc
     1021 tcgtcaattt tagcgggcat accatagagg tgaacagcaa ttattgcttt gggctttttc
     1081 ccctttgcaa tcctgtcctt aatggcaacc tcaagcaggt tggggtccat gttccatgtt
     1141 tcgggttcac tgtcaacaaa aacaggtgtt gccccttggt aaacaatggg gttggctgag
     1201 gccgaaaagg tgaacgactg gcacacaacc tcatcgccgt aggcaacccc aaggtttata
     1261 agagccatgt gaagtgctgc tgtaccagaa accagcgcag cgcaatgatt cactttaagg
     1321 taggaggcta actcctgctc aaatcccgta acattaggac ctaagggcga gacccagttg
     1381 gtatcaaatg cctcgttgat gtaccgcatc tcccgcccgc tcatgtgggg ggaggaaagc
     1441 caaattttag gattcataag ctgaatatca ttatatctaa ttattcttcc aggatttttt
     1501 attataacag cattgtccgg aacattttta gccataactg ccccagcacc tacaagacac
     1561 ctctttccaa tttatatttc cggaacaata ccggtctctg tatatattaa acaatggttt
     1621 tcaatatcca atctgcgtcc atccgcgtga aatctt
//
LOCUS       DAOSHM010000160         1618 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_78206_length_1618_cov_13.062060, whole genome shotgun
            sequence.
ACCESSION   DAOSHM010000160 DAOSHM010000000
VERSION     DAOSHM010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78206_length_1618_cov_13.062060"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            226..1503
                     /locus_tag="PK839_10915"
     CDS             226..1503
                     /locus_tag="PK839_10915"
                     /inference="COORDINATES: protein motif:HMM:NF033592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HOG73286.1"
                     /translation="MTQLLDDEEYHCRSKNGFPGVFSRDRKLTFKRLIVFIARGIRSG
                     VQRELDSFYREVVGSEFNIRAITKGAFTQARAKLNHGAFIELNESVNHTFYSEAPYLV
                     WHNMRLLSVDGTRLVLPKHKSVAAEFGEHGFGPNADSERSLALGSLLYDCLNLLTLDA
                     QLAPYASSERELLYKHLDKVGRGDLLLLDRGYPSIALLFLLTAKGVEFCVRMKEDWWL
                     SVKDFKESGEKQRIVSYTLPKKDKELLKGYPDVQGKAIRVRLVRVDLGGGGTEVLCTS
                     LLDAEKYTCDDIAELYHFRWNVEEGYKLYKARAELENFSGKTALAVRQDFFAKVFTMS
                     LCAVLAFPIEERVKKESARTGGKHSQKVNRTNAISMLRNISVALLLKQLVEPAIKAFD
                     LLVEKTTEIVRPGRKFERKKRTKKLYYMNYKQL"
BASE COUNT          459 a          361 c          445 g          353 t
ORIGIN      
        1 agctgttctt acttcgtaaa aataaaacaa acaaagaaga tgagtagtaa taatgcaatg
       61 tcgtttagtt aaggtcaaaa gggcggggaa tattaatatt ttagcaaaaa aggttcattg
      121 aaaaaatggg gtttcaggat aaaccattat ctttaggggt ataatatagg ggcctctggt
      181 aaagcctccg aaaacaagga ttttcgtgaa aacacagtta caaacataac ccaactactt
      241 gatgatgaag agtatcactg ccgttcaaaa aacggctttc caggggtatt cagccgtgac
      301 cggaagctga ccttcaagag gttaatcgtg tttattgcca ggggaattcg gagcggggtt
      361 cagcgggagc tggattcctt ctacagggaa gtcgttggct ccgagttcaa cattcgggca
      421 ataaccaagg gagccttcac ccaggcgcgg gcaaaattga accatggggc cttcatcgag
      481 ctaaacgaga gcgtcaacca cactttttac agcgaggcgc cctacctggt atggcataac
      541 atgcgcctac tgtcggtcga tggcacccgc ctggtgctac ccaagcacaa gagcgtagcc
      601 gccgagttcg gcgagcacgg ttttgggccc aacgcggaca gcgaacgctc cctggccctg
      661 ggctcactcc tgtacgattg cctaaacctg ctgaccctgg atgcgcaact tgcgccctat
      721 gccagcagcg agcgggagtt gctgtacaag cacctggata aggtcggcag gggggacctg
      781 ctgcttctcg acagggggta tccgagcatt gcccttttgt tcttgctgac ggccaaggga
      841 gttgagttct gcgtaaggat gaaggaagac tggtggctca gtgtaaaaga ttttaaggaa
      901 agcggggaaa agcagcgaat cgtgtcatac accctaccca aaaaggacaa ggagctgctg
      961 aagggctacc cggatgtaca aggtaaggca atacgcgtcc ggttagtccg ggtggatttg
     1021 gggggtggcg gaacagaagt gctgtgcacc tccctgctgg atgccgaaaa gtacacctgc
     1081 gatgatattg ccgagctgta ccatttccga tggaatgtgg aggaggggta caagctgtac
     1141 aaggcgcggg ctgagctgga gaacttctct gggaaaacgg cgctagcggt gaggcaagat
     1201 tttttcgcca aggtatttac gatgagtttg tgcgccgtgc tggctttccc catagaagaa
     1261 agggtaaaaa aggaatcggc acgcacgggt ggcaagcaca gccaaaaggt aaaccgcacc
     1321 aatgccatat ccatgctcag gaacataagc gtggccttgc tattaaagca gctggtggaa
     1381 cctgccatta aagcttttga cttgctcgtt gagaaaacaa cggaaatagt gaggccaggt
     1441 agaaaatttg agcgcaagaa acgaacgaag aagctctact atatgaatta caagcaatta
     1501 tagcttaact aaacgacatt gattcaaaat tcaagatttt aaaacttgta agcctactgg
     1561 cgaaacctgt ccgcctcccg gcggaacttg tccggctcaa aaggacttac aattaaac
//
LOCUS       DAOSHM010000161         1450 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_92561_length_1450_cov_17.513978, whole genome shotgun
            sequence.
ACCESSION   DAOSHM010000161 DAOSHM010000000
VERSION     DAOSHM010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1450
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92561_length_1450_cov_17.513978"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="PK839_10920"
     CDS             complement(<1..199)
                     /locus_tag="PK839_10920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG73287.1"
                     /translation="MNIPADQYQISKNTVFKWKNRNGQDDRSSRPHTIHYYLSALEKE
                     IINSARRSSWLPLEEITEVAQQ"
     gene            421..>1450
                     /locus_tag="PK839_10925"
     CDS             421..>1450
                     /locus_tag="PK839_10925"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HOG73288.1"
                     /translation="MKLRVGNLLLAMMLMALPSSPCTIIAVGKKASADGSNIISHTDA
                     GPDSRIFVVPAKSFKPGTMAPVYWGIQDADRPLNDDGIIVGYIPQVEKTYKYFQSAYS
                     HVNEYQLGIAESTTDQRRELFCNRDNGKQIMTIEQAQIFALQRYKTAREAVRFIGSLM
                     TRYGFLPSSGDGSETLVIADTEEIWVLEVFGVGPGWTPGSGKPGAIWAAQRLPEDEAT
                     IVPNWSIIKEIDASDTLNFMVSDNYMQEAIDRGWYNPASGKPFIWQEAYAPLPAEYAT
                     GRFWLFYQTFAPNLKEWPNRSAEGNPYKGITPYYQIVEPISLYPFSTKPEKKISVQDV
                     IAFQRSTFE"
BASE COUNT          375 a          338 c          354 g          383 t
ORIGIN      
        1 cctgctgtgc cacttccgta atttcttcca acggtagcca tgaacttctg cgggcggagt
       61 taataatttc cttttccaga gcggaaaggt agtaatgaat ggtgtgaggt cgggaacttc
      121 tgtcgtcttg tccattgcgg tttttccact taaagaccgt gtttttggaa atctgatact
      181 gatctgccgg tatgttcatt ggcagattac ttttttgcag ctcgcaacga atgtgtacat
      241 ttgtcgttgc gttgaaatga tacccttgca taggttgtaa atttttatgg ttaataattt
      301 acaaagcaag gtgtttttcc cgagttaagt tttcccgccc tgcagggcgg gaaaacttaa
      361 ccttcacatc caacaacgtg gtgaaacttt acagttacta acccaaaatt tacgattaaa
      421 atgaaattaa gagtaggaaa cttgctcctg gcaatgatgc ttatggcatt gccatcgagt
      481 ccctgcacca taattgcagt gggtaaaaag gcctcggccg atggttcaaa catcatctcg
      541 cacaccgatg cgggacccga ctcccggatt tttgtagttc cggccaaatc atttaagcct
      601 ggaacaatgg ctcctgttta ctggggaatt caggatgccg acaggcctct aaacgacgat
      661 ggaataattg tggggtacat tccgcaggtt gaaaaaactt acaaatactt tcaatcggcc
      721 tactcacatg ttaacgagta ccagcttggg atagccgaaa gcactaccga ccagcgcagg
      781 gagctgttct gcaaccggga taacggtaag cagataatga ctattgagca ggcccagata
      841 tttgcccttc agcgctacaa aaccgcacgg gaggcggtgc gattcattgg cagcctgatg
      901 actcggtacg ggtttcttcc atctagtgga gatggatccg aaaccttggt aattgcagat
      961 accgaggaga tttgggtgct tgaggttttt ggcgttggcc ctgggtggac gcccggcagc
     1021 ggaaagcctg gtgctatctg ggcagcccaa aggcttcccg aagatgaggc caccattgtt
     1081 cctaactgga gcattatcaa ggaaattgat gccagcgaca cgctaaactt catggtttcg
     1141 gacaactaca tgcaggaggc tattgatagg ggttggtata acccagcttc aggcaagcca
     1201 ttcatctggc aggaagccta tgcaccccta cctgctgagt acgcaaccgg gagattttgg
     1261 cttttctacc aaacatttgc cccaaacctt aaggaatggc ccaaccgtag tgctgagggt
     1321 aatccctaca agggaataac cccttactac cagattgttg aaccgatttc gctttacccg
     1381 ttctcgacta aaccggagaa aaaaatctcc gttcaggatg ttatagcatt tcagcggtca
     1441 accttcgagg
//
LOCUS       DAOSHM010000162         1271 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_113380_length_1271_cov_17.237664, whole genome shotgun
            sequence.
ACCESSION   DAOSHM010000162 DAOSHM010000000
VERSION     DAOSHM010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1271
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113380_length_1271_cov_17.237664"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..689)
                     /locus_tag="PK839_10930"
     CDS             complement(<1..689)
                     /locus_tag="PK839_10930"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOG73289.1"
                     /translation="MAKRLTRREFIRNGLLAAGGLYLTRFTSLRATAAGNQKPWKWSR
                     EAMFYTLTPRGARCEICPNECTIRPGESGDCRNRENTNGKLYSIAYGNPCSVHVDPIE
                     KKPLYHFLPGSQSFSIATAGCNFACMNCQNWEISQTSPRKTRNIELFPEQVVEQCLLS
                     GSKTIAYTYSEPTSFYEYMFDTAVIAHRRGVRNVLVSNGYMNDKPIRTLAPYLDAANI
                     DLKSFSNDIYL"
BASE COUNT          314 a          301 c          307 g          349 t
ORIGIN      
        1 ttcaggtaaa tgtcgttgct gaacgatttc aggtcgatgt tggcagcgtc gaggtagggt
       61 gcaagcgtgc gtatgggctt gtcgttcatg tatccattgg aaaccagcac gtttctaacc
      121 cctcgccggt gtgcaatcac agcggtgtcg aacatatact cgtaaaatga ggtcggctcg
      181 gagtaagtgt aggcaatggt cttcgatccg gatagcaggc attgctccac cacctgctcg
      241 gggaagagtt caatattccg ggtttttcga gggctggttt gtgaaatttc ccagttctgg
      301 cagttcatac aggcaaagtt gcatccggca gtagcgattg aaaaggactg cgaaccgggc
      361 aaaaagtggt aaagaggctt tttttcgatt gggtccacat gaaccgagca ggggttgcca
      421 taggcaatgg agtaaagctt gccgttggtg ttctccctgt tgcgacagtc gcccgattcc
      481 ccgggacgaa tagtgcactc gttagggcat atttcacacc tcgcaccccg tggggttagc
      541 gtgtaaaaca tagcctccct gctccatttc catggctttt ggttgccggc agccgttgcc
      601 cttagacttg taaaacgggt aagatataaa cctcccgctg ccagcagccc gttcctgata
      661 aactcacgtc gtgttaacct tttagccacg ctacctagct atacgtacac aaaatgttac
      721 ccaaaactta cctcataacg gtatcattag ccctacctga ggtgggcaat aaagttaacc
      781 attaccatac atttaagcaa atttgggggg gtaacccctg ccagacaacg gcagcaaaga
      841 accatataca gagaagctgg tcggtaccga taataggcgt agcttccaaa attcgtcagg
      901 taaactccct tgctgccgct ggacttccgt cgtgtgggag caagagggtc tgttatgcgc
      961 tttctcataa tggctgaggc catatacagt aaattattgg gtggttacct ctcttcccaa
     1021 atgcacctaa tttagatttg acagaacgta caaattatca atgaaaccct tatggtatat
     1081 gggcttagtt atgtctttgt tcattgtttt catccgtata aaatctaata ttattaattg
     1141 acaataactt acgatttcgc cttaagcact cgttaacgtt cggatggaac accaatattt
     1201 tattggcttc gccgagctct ccgccagctg gcggatcggt tcatccctgt ttgtgcaagc
     1261 tgccttacat g
//
LOCUS       DAOSHM010000163         1244 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_117324_length_1244_cov_10.120269, whole genome shotgun
            sequence.
ACCESSION   DAOSHM010000163 DAOSHM010000000
VERSION     DAOSHM010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1244
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117324_length_1244_cov_10.120269"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..778
                     /locus_tag="PK839_10935"
     CDS             <1..778
                     /locus_tag="PK839_10935"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOG73290.1"
                     /translation="LLKIRTFGRNSLLNFLSRYSEPQPGIHILNGHFLSLDGKSSPQM
                     FEELLSGMEDRGVRFINFEEAVKRITANIFPVDECLVAFSWDDGFEECFTRIKPVLDR
                     RSLKAAFFINPGFIDGDEAYRYHFTHEVVMVDKPPMSWEQLRILSSEGHVVGAHTLDH
                     LSLDSDNTELLHRQIAGSKIRIEDKINARVEHFAFPFGRLKLLSPLGLQLALSNFKYV
                     YSQSNYRHYFSFDGKVINRRHFECDWPLEHVIYFLKSKKF"
     gene            775..>1244
                     /locus_tag="PK839_10940"
     CDS             775..>1244
                     /locus_tag="PK839_10940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG73291.1"
                     /translation="MRAKVDVLVVGDFPPRTFTGISMVNALVKGTLEAKGKIVVAIDE
                     SAWRLKGLGRIFHYFSRYVDVARPLVSCRVEILYTNIPLSRFGLLRLILLCFFARIIS
                     WKTTFYGHIHRGDIRSFFTSSHVNRFLLKLSLSFFNRIIVLSKQFADEVKAINS"
BASE COUNT          313 a          260 c          291 g          380 t
ORIGIN      
        1 acttcttaaa ataagaacct ttggacgaaa ttcactcctg aacttcttgt cgcgttactc
       61 agagccccaa cctggcatcc atattctcaa cggtcacttt ttaagccttg atggcaaatc
      121 ctcgcctcaa atgttcgagg agctactttc gggcatggag gataggggtg tgaggttcat
      181 taactttgag gaggcggtca aaaggattac agccaatatt tttccagtgg atgaatgcct
      241 ggtggccttc tcctgggatg atggctttga ggagtgcttt acccgaatta agccggtttt
      301 agataggcgc agccttaaag ccgctttttt tattaacccg ggtttcattg acggcgatga
      361 agcctaccgc taccacttta cccatgaggt ggttatggta gataagccac ccatgagctg
      421 ggagcagctt agaatactaa gttcagaggg ccatgtggtt ggcgcccaca cgctcgacca
      481 tctatcgctt gattctgata ataccgagtt gctccatagg cagatagctg gctcaaaaat
      541 taggatagag gataaaatca atgctagggt tgagcatttt gccttcccct tcggacggct
      601 gaagctgcta agccccttag gacttcagct tgcactctcc aattttaagt atgtttacag
      661 ccagagcaac taccgtcatt acttctcttt cgacggaaag gttattaacc gtcgacactt
      721 cgagtgcgat tggcctttgg agcatgttat ttactttcta aaatctaaaa agttttgagg
      781 gcaaaggttg acgtgcttgt ggtgggagat tttccaccca gaacttttac cggcattagc
      841 atggttaatg ccttagtgaa gggaacgttg gaggctaaag ggaaaatagt tgttgctatt
      901 gacgaatcgg catggaggtt aaaagggtta ggtcgcatct ttcattactt ttcaaggtat
      961 gttgatgttg cccggccgct ggttagctgt cgggtcgaaa tcctttacac taacattccc
     1021 ctatcaaggt ttggattact ccgattgatt ttgctttgct tttttgctcg cataatttca
     1081 tggaaaacga ctttttacgg gcatatccac aggggggata tacgttcatt ttttacatca
     1141 tcccacgtaa acaggtttct tttgaagcta tcattgagtt tttttaacag gattattgtt
     1201 cttagtaagc aatttgccga tgaagttaaa gcaatcaaca gcaa
//
LOCUS       DAOSHM010000164         1176 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_127916_length_1176_cov_12.149866, whole genome shotgun
            sequence.
ACCESSION   DAOSHM010000164 DAOSHM010000000
VERSION     DAOSHM010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1176
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127916_length_1176_cov_12.149866"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..971)
                     /locus_tag="PK839_10945"
     CDS             complement(<1..971)
                     /locus_tag="PK839_10945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG73292.1"
                     /translation="MSKFRENFLTVVLLLFAVRLGLPQYIKFALYPAVAFFGVWALVN
                     FVRSKGWRKVKLVQLGIFTPWILLLLYYAISLVFTPDKQVGLLKDAFNILVLAAFIVS
                     VFLAGYTKGELVKSLQRFGLLTVVFGALFALAGIVKLVLQLYGVRLGFLVPEGFGYPM
                     GTSLNIDDNFFTLLCLLGVVFVLPHAFSQLSKTNRVWLQVAVYLLVVNIFLATSRRGL
                     IIGSLFLTGAVLLWVVSWMHKTERLSCFRRNTTLLILFIGLTFGGYYYFVFRVSALER
                     NQWLANSRFNKGEFNTYLNLLTLNGQSIFKGNAQYTDVSDKIWQTTF"
BASE COUNT          398 a          278 c          225 g          275 t
ORIGIN      
        1 tcaaatgttg tttgccagat tttatcactc acatcggtat attgtgcatt gcccttgaaa
       61 atggactgcc cgttgagtgt taacaggttg aggtaggtat taaactcccc cttgttgaac
      121 cttgagtttg ccagccattg attcctttcc aaagcgctaa cacgaaaaac aaaataatag
      181 tatcctccaa atgtcagacc gataaaaagt atgagcaacg tggtattccg gcgaaagcag
      241 ctaagtcgtt cggttttgtg catccacgaa acaacccata gcaaaacagc acctgtaagg
      301 aagagtgagc caataataag ccctctgcgg gatgtggcta aaaatatgtt taccaccagc
      361 aggtaaacgg caacctgtaa ccaaacccga ttagttttac tcaactggga gaaggcatgg
      421 ggtaaaacaa aaacaactcc caaaaggcag agcagggtga aaaagttatc atcaatattt
      481 agggaggttc ccattggata gccaaaaccc tcgggtacaa ggaaccccaa cctaaccccg
      541 taaagctgca acaccagctt tacaataccg gcaagggcaa ataacgcgcc gaaaacaacc
      601 gttagtagtc caaaacgttg gagtgatttt acaagttcgc cctttgtgta cccagccaaa
      661 aaaaccgata cgataaaagc ggcaagcacc aggatattga aggcatcctt taggaggcct
      721 acctgcttat ccggagtaaa aaccagcgaa atggcatagt acagcagcag caaaatccat
      781 ggtgtaaaaa tgcccagctg caccaacttg acttttcgcc accccttaga acgtacaaaa
      841 ttaaccagcg cccaaacacc aaaaaacgca actgccgggt atagggcaaa ttttatgtac
      901 tggggtaaac ccagccttac tgcaaataaa agcaggacaa ccgtaagaaa attttctcgg
      961 aatttcgaca tgattttaac cattcgggta catgtaaaat aaattcagct tagcctatag
     1021 ctgattcaac aatcaaaata cccacatcat tgctcaatcg tcataaagta catccggaaa
     1081 tcagcaatag cctaacaccc agatagccat accttttaaa acgttaacca ccaccttttc
     1141 ttttatcatt tttggtcata tttttttaga aggcta
//
LOCUS       DAOSHM010000165         1116 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_138686_length_1116_cov_13.173421, whole genome shotgun
            sequence.
ACCESSION   DAOSHM010000165 DAOSHM010000000
VERSION     DAOSHM010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138686_length_1116_cov_13.173421"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..907)
                     /locus_tag="PK839_10950"
     CDS             complement(<1..907)
                     /locus_tag="PK839_10950"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOG73293.1"
                     /translation="MEKIIYGIQQVGLGVSNLEEAWKWYINLLGFDVKIFDDDTVAEL
                     MLPYTGGEPQQRHAILALNLQGGGGLEIWQYKERTPLPPKFPVKLGDLGIFAAKIKSP
                     DIEAAFNFLKSKGATLGDLVNSPEGKPHFYISDPFGNTLEVYEDKSTFLNEGRSTSGI
                     GGAIVGVSQMERSVKFYTKVLGYDRVVYNKSGKFDDFACLPAGNENFHRVLLTHSDTR
                     KGSFSELFGQSFIELVQPLDRVPVKILQDRFWGDLGFIHLCFDVKNMDQFERECNELG
                     FPFTVNSNIKHSDKGSFDMGEAAGHF"
     gene            complement(920..>1116)
                     /locus_tag="PK839_10955"
     CDS             complement(920..>1116)
                     /locus_tag="PK839_10955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="short-chain dehydrogenase"
                     /protein_id="HOG73294.1"
                     /translation="NEYGKMDPGQSNGLAPQKCAKRYIRAVKRRKPEILIGKSELLMV
                     HIKRLLPSLFYKMVSRVNPV"
BASE COUNT          279 a          291 c          249 g          297 t
ORIGIN      
        1 tgaaatggcc tgcagcctcg cccatatcaa aactaccctt atcgctatgc ttgatgttgg
       61 agttgacagt gaagggaaaa cccagctcgt tacactcccg ttcgaactgg tccatattct
      121 ttacatcaaa gcaaaggtga atgaagccaa ggtcgcccca aaaacggtcc tgcagaattt
      181 tcacgggaac tctatccagc ggttgtacaa gttcgatgaa cgactggccg aatagctcgc
      241 taaagctacc cttgcgggta tctgaatggg taagtagcac ccggtggaaa ttctcgtttc
      301 cggcaggaag gcaggcaaag tcatcgaact tgcctgattt gttgtaaaca acccggtcgt
      361 agcccagcac tttcgtgtaa aattttaccg acctctccat ctggcttacc ccaacaatgg
      421 ctccgccaat cccggaagtg cttcttccct catttaaaaa ggttgatttg tcctcgtaaa
      481 cctccagggt attgccaaag gggtcggata tgtagaaatg tggcttcccc tcaggagaat
      541 taaccaaatc gccaagtgtt gcgcctttgg atttcagaaa gttaaaggca gcctcgatat
      601 cgggactttt aattttagca gcaaaaattc ccaggtcgcc aagttttacc gggaactttg
      661 gaggaagagg agtccgctcc ttgtactgcc aaatctccaa gccaccgcct ccctggaggt
      721 tcagggcaag aattgcgtgc cgttgctggg gttcaccacc ggtgtaggga agcattagct
      781 cggctaccgt gtcgtcgtca aaaattttaa cgtcaaatcc caacaagttg atgtaccatt
      841 tccatgcttc ttccaggttg ctcaccccca ggccaacctg ctgaatgccg taaataattt
      901 tctccatgaa ttttaacttt tacacagggt tcaccctgct taccatttta tagaatagcg
      961 aggggagtaa acgtttaatg tgaaccatca gcagctcgct ttttccaatc aaaatctccg
     1021 gtttacgccg ctttacagca cggatatacc ttttggcaca cttttgcggt gccaggccgt
     1081 tgctctgccc ggggtccatt tttccatact cgttac
//
LOCUS       DAOSHM010000166         1106 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_140598_length_1106_cov_9.110371, whole genome shotgun
            sequence.
ACCESSION   DAOSHM010000166 DAOSHM010000000
VERSION     DAOSHM010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140598_length_1106_cov_9.110371"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..774
                     /locus_tag="PK839_10960"
     CDS             <1..774
                     /locus_tag="PK839_10960"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOG73295.1"
                     /translation="EEDCIERCIKSVSGVADEVVVVDSYSTDRTGEICRQLGVRFIQH
                     PFEGYREQKNYALQLATYDYILSLDADEALSEELRQSILNVKENWGADGYYFNRLNNY
                     CGQWIHHSNWYPDRKLRLFDRRKGCWGGINPHDRVVMQKGSRVHYLKGDLLHWVLNSY
                     EAHIDKANKFSTIAAHEYFKLGRKSSMPKIILNSTWRFFKAYVLRLGFMDGFNGFVIS
                     SFSAYTSFLKYVKLHQLNRSEKEVKSKPSAKNRNNKIAS"
BASE COUNT          339 a          230 c          245 g          292 t
ORIGIN      
        1 gaggaggatt gcattgaacg ctgtataaaa tcagtttcgg gggtggccga tgaggttgta
       61 gtggttgact cctactccac cgaccggacg ggtgaaattt gccggcagct gggagttagg
      121 tttatccagc acccctttga gggctaccgc gagcagaaga actacgccct gcagcttgca
      181 acctatgact acattctgtc tctcgatgcc gatgaggccc tttcggaaga gcttaggcaa
      241 tccatactaa atgtaaagga gaactggggt gctgatggct actactttaa ccggttaaat
      301 aactactgtg gacaatggat tcaccattca aactggtacc ccgacaggaa gctcaggctt
      361 tttgaccgga ggaaaggctg ctggggtggc ataaaccctc atgaccgggt agttatgcaa
      421 aagggaagca gggtgcatta tcttaagggc gatttgctcc attgggtact caacagctac
      481 gaggcacata tagacaaggc caacaagttt agcaccattg cagcacatga atacttcaag
      541 ctgggaagga aaagcagcat gcccaaaatc attctcaaca gcacctggcg attttttaag
      601 gcgtacgtcc taaggttagg ttttatggat ggttttaacg gttttgtaat aagttcattt
      661 tcagcatata ccagcttttt aaaatacgta aagttgcatc agcttaacag aagcgaaaaa
      721 gaggttaaat caaagccatc agcaaaaaac aggaataata aaattgcctc ttaaccttaa
      781 aacctgctag tctactggac ttatatttcc ctgcatattt tttcgtactt gcagaaatag
      841 ctacgtttgc tttccaaaga aatatcgctc aatgctaaaa taaccaattt ttcgaaacgc
      901 cattaggttc tttaaggttt gattgtttat attaggcaac ctacctaaaa tgacctaaac
      961 gaaaaccgaa tattttattg acaaattgta taaccgatac taaacatttc taagaatcat
     1021 gaaaatagta atactggcag ccggcatagg ctcaaggttg gggaatccgc ttcccaaacc
     1081 gcttacagtt ctgcaaagcg gtaaaa
//
LOCUS       DAOSHM010000167         1035 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_156137_length_1035_cov_19.164286, whole genome shotgun
            sequence.
ACCESSION   DAOSHM010000167 DAOSHM010000000
VERSION     DAOSHM010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_156137_length_1035_cov_19.164286"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            308..895
                     /locus_tag="PK839_10965"
     CDS             308..895
                     /locus_tag="PK839_10965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG73296.1"
                     /translation="MRKLLFTLALAVLSQVSFGQAVNDQAIIPVSVTLNSILRLTVVS
                     GGNIDFVVNTIDQYTSGINFGGGDARWQTNFTVSSSQDFDVTLAAEDGNLMGQDDPTN
                     TMLLDNIGFDLTGSGGGPANWALPGDVLPLTNGPQSIVTGLAGASAGDATQNNFTIQW
                     RLGTSEDAMNGSSLLAQSLTPDRYITNVFLELVPK"
BASE COUNT          224 a          265 c          244 g          302 t
ORIGIN      
        1 tacaaggggc tgcgcccctt gctacttatt gcgccccttc ggggcttaaa acaccggaca
       61 agttccgtcg ttagccaggc aattttttat cctttaccct ttatccttta tccttgcctt
      121 tatccttgtc ctttcttctc cttgtttcaa aacttaattt gccctgttca tcgcctgatt
      181 ggttgtttct atcaactaat ccttttgaat tgttgcattt ttcgttatct ttaccatacc
      241 tttacgttga aaataacaaa atacctttac tatagggtat ttatctacta acttaaacat
      301 gaaagctatg agaaaactac tcttcaccct ggctttagca gtgcttagcc aggttagctt
      361 tggccaggca gttaacgacc aggccattat ccccgtttcg gttaccctca actccattct
      421 gaggctaacc gtggtttcgg gtggcaacat cgactttgtg gtgaacacca ttgaccagta
      481 caccagtggc attaactttg ggggtggcga tgcccgctgg cagaccaact tcaccgtttc
      541 ctcctcgcag gactttgatg ttaccctggc ggcagaggat ggaaacctga tggggcagga
      601 tgacccgact aataccatgc tgctggacaa cattgggttt gacctgactg gatcgggagg
      661 tggccctgca aactgggcat tacctggaga tgtgcttcca cttaccaacg gtcctcagag
      721 tattgttaca gggctagctg gtgcttcagc tggcgatgcc actcagaaca acttcaccat
      781 tcagtggagg ctgggaacca gtgaggatgc aatgaacggc tcatccctgt tagcgcagag
      841 cttaaccccc gaccgctaca tcaccaacgt gttcctggag ctggtgccta aatagccggt
      901 ttccacatgg gccaatggcc ctggtataat ccgtacggtt tggccgccgg gctgtttata
      961 tgcggcccgg agtgctgtgt atggagggtg gtttgtaact tttactgcta tggttgtcaa
     1021 ctcaacgggt atgga
//
LOCUS       DAOSHM010000168         1022 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_1727
            NODE_159250_length_1022_cov_18.617373, whole genome shotgun
            sequence.
ACCESSION   DAOSHM010000168 DAOSHM010000000
VERSION     DAOSHM010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863256
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:30:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,156
            Genes (coding)                    :: 2,149
            CDSs (with protein)               :: 2,149
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 2 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1022
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_159250_length_1022_cov_18.617373"
                     /isolate="AMR_MDS_1727"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1022
                     /locus_tag="PK839_10970"
     CDS             <1..>1022
                     /locus_tag="PK839_10970"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HOG73297.1"
                     /translation="LTDTLRAQPDLVYEYFIAEIGKQSPIDFDYNPHVRRYIDVYTTE
                     RREQVSQMLGLAELYFPLFDEYLDKYHLPLELKYLAAVESALNPLAVSKTGAAGIWQF
                     KINTARMFDLKVSSYVDERMDPDKSTEAACRYLEYLYRTFNSWHLAIAAYNVGPEPIR
                     KAIARNHGETNFWKLYDDLPESAQNYVPAFIAAAYVMQHYGDHGIFPARPTIAFAQTD
                     TVHVAFAVNFSTLSSALNIPVELLRYLNPMYRIDYIPKDSQQQVLRLPSDKAVLFTAN
                     KESILFNSPKTTHQQSITSGSASGSSKKSKMLIYTVKDGDSINKIAINYYCTLDLIRQ
                     WNPGLDT"
BASE COUNT          280 a          261 c          209 g          272 t
ORIGIN      
        1 ctgaccgaca cgctgcgggc tcaacccgac ctggtttacg agtactttat tgccgaaatt
       61 gggaagcaat cgcctattga ttttgactac aatcctcatg tgaggaggta cattgacgtt
      121 tacactactg aacgcaggga acaggtgtcg cagatgctgg ggcttgctga gctatacttt
      181 cccctgttcg atgagtacct cgataagtac cacctgccct tagaactaaa ataccttgct
      241 gctgtcgaat cggctcttaa tcccctggcc gtttctaaaa ccggtgctgc aggtatttgg
      301 cagttcaaaa tcaatactgc gcgaatgttc gatttaaagg tttcatccta cgttgatgag
      361 cggatggatc cggataaatc aaccgaagca gcatgccgtt acctggagta cctttacagg
      421 acatttaata gctggcattt agccattgca gcctataatg ttggaccaga acctataaga
      481 aaagctattg cacgaaatca tggtgaaacc aacttttgga agctttacga tgatctgcct
      541 gaatcagctc aaaactatgt tcctgccttt attgctgccg catacgtaat gcagcattat
      601 ggcgaccacg gcatttttcc tgctcgccct acaattgcct ttgcccaaac tgataccgtt
      661 catgtagcct ttgccgtcaa cttctccaca ctatcttcgg cgcttaacat cccagtggaa
      721 cttctccggt acctgaaccc catgtaccgg atcgactata tccccaaaga cagccaacag
      781 caggttttac ggctgccatc cgataaagct gttttattta cagctaataa ggaatccata
      841 cttttcaact cacccaaaac aacccatcag cagtccataa cttccgggtc agcaagtgga
      901 agttcaaaaa aatcgaaaat gctaatctat acggttaagg atggggatag tataaacaag
      961 attgccatca actactactg cacgcttgac ctgattaggc aatggaatcc tggccttgac
     1021 ac
//
